PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 12551917-4 2003 SH2-Bbeta is tyrosyl-phosphorylated in response to GH and interferon-gamma, stimulators of JAK2, as well as in response to PDGF and nerve growth factor. cyclo(tyrosyl-tyrosyl) 13-20 growth hormone 1 Homo sapiens 51-53 16705159-6 2006 When Ser523 in JAK2 was mutated, JAK2 kinase activity as well as GH-dependent tyrosyl phosphorylation of JAK2 and Stat5 was enhanced, suggesting that phosphorylation of Ser523 inhibits JAK2 kinase activity. cyclo(tyrosyl-tyrosyl) 78-85 growth hormone 1 Homo sapiens 65-67 3349036-1 1988 Because many growth factor receptors are ligand-activated tyrosine protein kinases, the possibility that growth hormone (GH), a hormone implicated in human growth, promotes tyrosyl phosphorylation of its receptor was investigated. cyclo(tyrosyl-tyrosyl) 173-180 growth hormone 1 Homo sapiens 105-119 3349036-1 1988 Because many growth factor receptors are ligand-activated tyrosine protein kinases, the possibility that growth hormone (GH), a hormone implicated in human growth, promotes tyrosyl phosphorylation of its receptor was investigated. cyclo(tyrosyl-tyrosyl) 173-180 growth hormone 1 Homo sapiens 121-123 26421979-2 2016 We showed that GH binding to its membrane-bound receptor enhances binding of JAK2 to the GHR, activates JAK2, and stimulates tyrosyl phosphorylation of both JAK2 and GHR. cyclo(tyrosyl-tyrosyl) 125-132 growth hormone 1 Homo sapiens 15-17 11751854-10 2002 In contrast to SH2-B beta, APS decreased tyrosyl phosphorylation of GH-stimulated JAK2 as well as Stat5B, a substrate of JAK2. cyclo(tyrosyl-tyrosyl) 41-48 growth hormone 1 Homo sapiens 68-70 11751854-13 2002 Consistent with these data, in 3T3-F442A adipocytes, endogenous APS was tyrosyl-phosphorylated in response to GH and interferon-gamma. cyclo(tyrosyl-tyrosyl) 72-79 growth hormone 1 Homo sapiens 110-112 10377387-7 1999 More importantly, SH2-Bbeta enhanced GH-induced tyrosyl phosphorylation of endogenous JAK2 and ligand-induced tyrosyl phosphorylation of Stat5B by endogenous JAK2. cyclo(tyrosyl-tyrosyl) 48-55 growth hormone 1 Homo sapiens 37-39 11460315-4 2001 Inhibition of tyrosyl phosphorylation of GH receptor-associated tyrosine kinase by tyrphostin-23 or staurosporine blocked GH-stimulated monocyte migration down to random levels. cyclo(tyrosyl-tyrosyl) 14-21 growth hormone 1 Homo sapiens 41-43 10644756-5 2000 PDGF substantially reduces tyrosyl phosphorylation of JAK2 induced by GH but not interferon-gamma or leukemia inhibitory factor. cyclo(tyrosyl-tyrosyl) 27-34 growth hormone 1 Homo sapiens 70-72 10644756-7 2000 The inhibitory effect of PDGF on GH-induced tyrosyl phosphorylation of JAK2 and GHR is abolished by depletion of 4beta-phorbol 12-myristate 13-acetate (PMA)-sensitive PKCs with chronic PMA treatment and is severely inhibited by GF109203X, an inhibitor of PKCs. cyclo(tyrosyl-tyrosyl) 44-51 growth hormone 1 Homo sapiens 33-35 10644756-9 2000 LPA, a known activator of PKC, also inhibits GH-induced tyrosyl phosphorylation of JAK2 and GHR and reduces the number of GHR. cyclo(tyrosyl-tyrosyl) 56-63 growth hormone 1 Homo sapiens 45-47 11036942-4 2000 In the intervening six years, a variety of signaling molecules have been identified that are tyrosyl phosphorylated in response to GH, presumably by the activated JAK2. cyclo(tyrosyl-tyrosyl) 93-100 growth hormone 1 Homo sapiens 131-133 11036942-9 2000 GH-induced binding of SH2-B to JAK2 via this site potently activates JAK2, leading to enhanced tyrosyl phosphorylation of Stat proteins and other cellular proteins. cyclo(tyrosyl-tyrosyl) 95-102 growth hormone 1 Homo sapiens 0-2 10842184-2 2000 We demonstrated previously that growth hormone (GH) induces tyrosyl phosphorylation of SIRPalpha and association of SIRPalpha with SHP-2. cyclo(tyrosyl-tyrosyl) 60-67 growth hormone 1 Homo sapiens 32-46 10842184-2 2000 We demonstrated previously that growth hormone (GH) induces tyrosyl phosphorylation of SIRPalpha and association of SIRPalpha with SHP-2. cyclo(tyrosyl-tyrosyl) 60-67 growth hormone 1 Homo sapiens 48-50 10842184-3 2000 The GH-activated tyrosine kinase JAK2 associates with and tyrosyl-phosphorylates SIRPalpha1. cyclo(tyrosyl-tyrosyl) 58-65 growth hormone 1 Homo sapiens 4-6 10842184-6 2000 GH-dependent tyrosyl phosphorylation of JAK2 is reduced when wild-type SIRPalpha1 compared with SIRPalpha1 lacking the four cytoplasmic tyrosines (SIRP 4YF) is expressed in cells, suggesting that SIRPalpha1 negatively regulates GHR/JAK2 signaling. cyclo(tyrosyl-tyrosyl) 13-20 growth hormone 1 Homo sapiens 0-2 10377387-2 1999 SH2-Bbeta was previously identified as a JAK2-interacting protein that is tyrosyl phosphorylated in response to GH and other cytokines that activate JAK2. cyclo(tyrosyl-tyrosyl) 74-81 growth hormone 1 Homo sapiens 112-114 10377387-7 1999 More importantly, SH2-Bbeta enhanced GH-induced tyrosyl phosphorylation of endogenous JAK2 and ligand-induced tyrosyl phosphorylation of Stat5B by endogenous JAK2. cyclo(tyrosyl-tyrosyl) 110-117 growth hormone 1 Homo sapiens 37-39 9507023-3 1998 In 3T3-F442A fibroblasts, GH rapidly stimulates tyrosyl phosphorylation of both SIRPalpha and SHP-2 and enhances association of SHP-2 with SIRPalpha. cyclo(tyrosyl-tyrosyl) 48-55 growth hormone 1 Homo sapiens 26-28 9507023-0 1998 Growth hormone regulation of SIRP and SHP-2 tyrosyl phosphorylation and association. cyclo(tyrosyl-tyrosyl) 44-51 growth hormone 1 Homo sapiens 0-14 9507023-4 1998 Consistent with JAK2 binding and phosphorylating SIRPalpha in response to GH, co-expression of SIRPalpha and JAK2 in COS cells results in tyrosyl phosphorylation of SIRPalpha and JAK2 association with SIRPalpha. cyclo(tyrosyl-tyrosyl) 138-145 growth hormone 1 Homo sapiens 74-76 7588209-5 1995 Dexamethasone (100 nM) for 24 h decreases by 50-75% GH-induced tyrosyl phosphorylation of mitogen-activated protein kinases ERK1 and ERK2, the transcription factor Stat3/APRF, the GH receptor-associated tyrosine kinase JAK2, and the GH receptor. cyclo(tyrosyl-tyrosyl) 63-70 growth hormone 1 Homo sapiens 52-54 8910607-1 1996 In this report, we demonstrate that insulin receptor substrate-2 (IRS-2) is tyrosyl-phosphorylated following stimulation of 3T3-F442A fibroblasts with growth hormone (GH), leukemia inhibitory factor and interferon-gamma. cyclo(tyrosyl-tyrosyl) 76-83 growth hormone 1 Homo sapiens 151-165 8910607-1 1996 In this report, we demonstrate that insulin receptor substrate-2 (IRS-2) is tyrosyl-phosphorylated following stimulation of 3T3-F442A fibroblasts with growth hormone (GH), leukemia inhibitory factor and interferon-gamma. cyclo(tyrosyl-tyrosyl) 76-83 growth hormone 1 Homo sapiens 167-169 8910607-4 1996 Characterization of the mechanism of signaling utilized by GH indicated that tyrosine residues in GH receptor are not necessary for tyrosyl phosphorylation of IRS-2; however, the regions of GH receptor necessary for IRS-2 tyrosyl phosphorylation are the same as those required for JAK2 association and tyrosyl phosphorylation. cyclo(tyrosyl-tyrosyl) 222-229 growth hormone 1 Homo sapiens 59-61 8910607-4 1996 Characterization of the mechanism of signaling utilized by GH indicated that tyrosine residues in GH receptor are not necessary for tyrosyl phosphorylation of IRS-2; however, the regions of GH receptor necessary for IRS-2 tyrosyl phosphorylation are the same as those required for JAK2 association and tyrosyl phosphorylation. cyclo(tyrosyl-tyrosyl) 222-229 growth hormone 1 Homo sapiens 59-61 8805024-1 1996 Demonstration that the tyrosine kinase JAK2 is activated in response to growth hormone (GH) has established tyrosyl phosphorylation as a signalling mechanism for GH. cyclo(tyrosyl-tyrosyl) 108-115 growth hormone 1 Homo sapiens 72-86 8805024-1 1996 Demonstration that the tyrosine kinase JAK2 is activated in response to growth hormone (GH) has established tyrosyl phosphorylation as a signalling mechanism for GH. cyclo(tyrosyl-tyrosyl) 108-115 growth hormone 1 Homo sapiens 88-90 8805024-1 1996 Demonstration that the tyrosine kinase JAK2 is activated in response to growth hormone (GH) has established tyrosyl phosphorylation as a signalling mechanism for GH. cyclo(tyrosyl-tyrosyl) 108-115 growth hormone 1 Homo sapiens 162-164 8615823-7 1996 In addition, several tyrosyl-phosphorylated proteins were specifically adsorbed from lysates of cells exposed to GH by a glutathione S-transferase fusion protein containing the 85 kDa regulatory subunit of PI 3-kinase. cyclo(tyrosyl-tyrosyl) 21-28 growth hormone 1 Homo sapiens 113-115 8815791-3 1996 The model presented is one in which GH binding to two GHRs causes dimerization of GHR, activation of the GHR-associated JAK2 tyrosine kinase, and tyrosyl phosphorylation of both JAK2 and GHR. cyclo(tyrosyl-tyrosyl) 146-153 growth hormone 1 Homo sapiens 36-38 7782332-0 1995 Growth hormone, interferon-gamma, and leukemia inhibitory factor promoted tyrosyl phosphorylation of insulin receptor substrate-1. cyclo(tyrosyl-tyrosyl) 74-81 growth hormone 1 Homo sapiens 0-14 1537311-1 1992 We have shown previously that GH receptors (GHRs) become phosphorylated on tyrosyl residues when GH-responsive cells are exposed to GH. cyclo(tyrosyl-tyrosyl) 75-82 growth hormone 1 Homo sapiens 30-32 7535773-0 1995 Growth hormone-promoted tyrosyl phosphorylation of SHC proteins and SHC association with Grb2. cyclo(tyrosyl-tyrosyl) 24-31 growth hormone 1 Homo sapiens 0-14 7535773-4 1995 Human GH was shown to promote the rapid tyrosyl phosphorylation of 66-, 52-, and 46-kDa SHC proteins in 3T3-F442A fibroblasts. cyclo(tyrosyl-tyrosyl) 40-47 growth hormone 1 Homo sapiens 6-8 7535773-12 1995 These findings suggest that GH stimulates: 1) the association of SHC proteins with JAK2.GHR complexes via the SHC-SH2 domain, 2) tyrosyl phosphorylation of SHC proteins, and 3) subsequent Grb2 association with SHC proteins. cyclo(tyrosyl-tyrosyl) 129-136 growth hormone 1 Homo sapiens 28-30 7517045-2 1994 Studies showing that GH rapidly stimulates tyrosyl phosphorylation of cellular proteins, and that tyrosine kinase activity co-purifies with GH-GH receptor complexes, led us to hypothesize that activation by GH of a receptor-associated tyrosine kinase is an important early, and perhaps, initiating step in signal transduction by GH. cyclo(tyrosyl-tyrosyl) 43-50 growth hormone 1 Homo sapiens 21-23 7508925-3 1994 We report that in 3T3-F442A fibroblasts, GH stimulates tyrosyl phosphorylation of a protein recognized by antibody to p91, a component of DNA-binding complexes that are activated by tyrosyl phosphorylation in response to interferons alpha and gamma. cyclo(tyrosyl-tyrosyl) 55-62 growth hormone 1 Homo sapiens 41-43 7508925-3 1994 We report that in 3T3-F442A fibroblasts, GH stimulates tyrosyl phosphorylation of a protein recognized by antibody to p91, a component of DNA-binding complexes that are activated by tyrosyl phosphorylation in response to interferons alpha and gamma. cyclo(tyrosyl-tyrosyl) 182-189 growth hormone 1 Homo sapiens 41-43 8385106-4 1993 Over a physiologically relevant range of concentrations, GH was found to promote, in 3T3-F442A fibroblasts, rapid changes in the level of tyrosyl phosphorylation of more than 13 proteins. cyclo(tyrosyl-tyrosyl) 138-145 growth hormone 1 Homo sapiens 57-59 8385106-5 1993 At the highest GH concentration employed (500 ng/ml), increased tyrosyl phosphorylation of two proteins, pp121 and pp97, was clearly visible at 1 min, the earliest time tested. cyclo(tyrosyl-tyrosyl) 64-71 growth hormone 1 Homo sapiens 15-17 8385106-6 1993 Increased tyrosyl phosphorylation of a number of other proteins (pp250, pps160-180, pps140-160, pp130, pp90, pp75, pp45, pp42, pp39, and pp36) and decreased tyrosyl phosphorylation of a 140-kDa protein were apparent after 5-10 min of incubation with GH. cyclo(tyrosyl-tyrosyl) 10-17 growth hormone 1 Homo sapiens 250-252 8385106-8 1993 When added to anti-GH antibody immunoprecipitates from GH-treated cells, they inhibited incorporation of 32P from [gamma-32P]ATP into tyrosyl residues in GH receptor complexes. cyclo(tyrosyl-tyrosyl) 134-141 growth hormone 1 Homo sapiens 19-21 8385106-8 1993 When added to anti-GH antibody immunoprecipitates from GH-treated cells, they inhibited incorporation of 32P from [gamma-32P]ATP into tyrosyl residues in GH receptor complexes. cyclo(tyrosyl-tyrosyl) 134-141 growth hormone 1 Homo sapiens 55-57 8385106-9 1993 When added to cells, all three inhibitors blocked all GH-dependent increases in tyrosyl phosphorylation of cellular proteins. cyclo(tyrosyl-tyrosyl) 80-87 growth hormone 1 Homo sapiens 54-56 1537311-9 1992 The increased tyrosyl phosphorylation of GHR detected after incubation of cells with GH is consistent either with GH increasing tyrosine kinase activity associated with the GHR or with GH inducing a conformational change in GHR that renders it susceptible to tyrosyl phosphorylation. cyclo(tyrosyl-tyrosyl) 14-21 growth hormone 1 Homo sapiens 41-43 1537311-9 1992 The increased tyrosyl phosphorylation of GHR detected after incubation of cells with GH is consistent either with GH increasing tyrosine kinase activity associated with the GHR or with GH inducing a conformational change in GHR that renders it susceptible to tyrosyl phosphorylation. cyclo(tyrosyl-tyrosyl) 14-21 growth hormone 1 Homo sapiens 85-87 1537311-9 1992 The increased tyrosyl phosphorylation of GHR detected after incubation of cells with GH is consistent either with GH increasing tyrosine kinase activity associated with the GHR or with GH inducing a conformational change in GHR that renders it susceptible to tyrosyl phosphorylation. cyclo(tyrosyl-tyrosyl) 14-21 growth hormone 1 Homo sapiens 85-87 1537311-9 1992 The increased tyrosyl phosphorylation of GHR detected after incubation of cells with GH is consistent either with GH increasing tyrosine kinase activity associated with the GHR or with GH inducing a conformational change in GHR that renders it susceptible to tyrosyl phosphorylation. cyclo(tyrosyl-tyrosyl) 259-266 growth hormone 1 Homo sapiens 41-43 1537311-9 1992 The increased tyrosyl phosphorylation of GHR detected after incubation of cells with GH is consistent either with GH increasing tyrosine kinase activity associated with the GHR or with GH inducing a conformational change in GHR that renders it susceptible to tyrosyl phosphorylation. cyclo(tyrosyl-tyrosyl) 259-266 growth hormone 1 Homo sapiens 85-87 1537311-9 1992 The increased tyrosyl phosphorylation of GHR detected after incubation of cells with GH is consistent either with GH increasing tyrosine kinase activity associated with the GHR or with GH inducing a conformational change in GHR that renders it susceptible to tyrosyl phosphorylation. cyclo(tyrosyl-tyrosyl) 259-266 growth hormone 1 Homo sapiens 85-87 7876144-5 1995 GH promotes in 3T3-F442A fibroblasts the tyrosyl phosphorylation of a protein immunoprecipitated by antibodies to Stat3. cyclo(tyrosyl-tyrosyl) 41-48 growth hormone 1 Homo sapiens 0-2 7876144-9 1995 The ability of GH to activate both Stat1 and Stat3 (i.e. increase their tyrosyl phosphorylation and ability to bind to DNA) suggests that gene regulation by GH involves multiple Stat proteins. cyclo(tyrosyl-tyrosyl) 72-79 growth hormone 1 Homo sapiens 15-17 7876144-9 1995 The ability of GH to activate both Stat1 and Stat3 (i.e. increase their tyrosyl phosphorylation and ability to bind to DNA) suggests that gene regulation by GH involves multiple Stat proteins. cyclo(tyrosyl-tyrosyl) 72-79 growth hormone 1 Homo sapiens 157-159 8343952-3 1993 Immunological approaches were used to establish GH-dependent complex formation between JAK2 and GHR, activation of JAK2 tyrosine kinase activity, and tyrosyl phosphorylation of both JAK2 and GHR. cyclo(tyrosyl-tyrosyl) 150-157 growth hormone 1 Homo sapiens 48-50 8385106-12 1993 In contrast, genistein and hydroxy-(2-naphthyl)-methylphosphonic acid, tyrosine kinase inhibitors lacking specificity for the GH receptor-associated kinase, decreased GH-dependent tyrosyl phosphorylation of only a subset of GH-responsive bands and partially blocked GH-dependent activation of MAP kinase. cyclo(tyrosyl-tyrosyl) 180-187 growth hormone 1 Homo sapiens 126-128 8385106-12 1993 In contrast, genistein and hydroxy-(2-naphthyl)-methylphosphonic acid, tyrosine kinase inhibitors lacking specificity for the GH receptor-associated kinase, decreased GH-dependent tyrosyl phosphorylation of only a subset of GH-responsive bands and partially blocked GH-dependent activation of MAP kinase. cyclo(tyrosyl-tyrosyl) 180-187 growth hormone 1 Homo sapiens 167-169 8385106-12 1993 In contrast, genistein and hydroxy-(2-naphthyl)-methylphosphonic acid, tyrosine kinase inhibitors lacking specificity for the GH receptor-associated kinase, decreased GH-dependent tyrosyl phosphorylation of only a subset of GH-responsive bands and partially blocked GH-dependent activation of MAP kinase. cyclo(tyrosyl-tyrosyl) 180-187 growth hormone 1 Homo sapiens 167-169 8385106-13 1993 These data show that increased tyrosyl phosphorylation of specific cellular proteins is a very rapid response to the binding of GH by the cell and most likely involves multiple tyrosine kinases. cyclo(tyrosyl-tyrosyl) 31-38 growth hormone 1 Homo sapiens 128-130 8385106-14 1993 Furthermore, inhibition of the GH receptor-associated tyrosine kinase blocks at least two actions of GH, the stimulation of tyrosyl phosphorylation of multiple proteins and MAP kinase activation. cyclo(tyrosyl-tyrosyl) 124-131 growth hormone 1 Homo sapiens 31-33 1537311-1 1992 We have shown previously that GH receptors (GHRs) become phosphorylated on tyrosyl residues when GH-responsive cells are exposed to GH. cyclo(tyrosyl-tyrosyl) 75-82 growth hormone 1 Homo sapiens 44-46 1537311-2 1992 In this work we investigate the molecular mechanism by which GH binding stimulates tyrosyl phosphorylation of GHR. cyclo(tyrosyl-tyrosyl) 83-90 growth hormone 1 Homo sapiens 61-63 1537311-5 1992 The rate of in vitro tyrosyl phosphorylation of GH-GHR complexes was not substantially reduced when the GHR preparation was diluted 1:10 or 1:100 before phosphorylation, consistent with a tight association between kinase and substrate (GHR). cyclo(tyrosyl-tyrosyl) 21-28 growth hormone 1 Homo sapiens 48-50 1537311-6 1992 When cells were labeled metabolically with 35S, and GHRs containing phosphorylated tyrosyl residues were isolated by immunoadsorption to and elution from phosphotyrosyl-binding antibody, the ability to immunoprecipitate 35S-labeled GHR with GHR antibodies was only evident when the cells had been incubated with GH. cyclo(tyrosyl-tyrosyl) 83-90 growth hormone 1 Homo sapiens 52-54 1537311-7 1992 This indicates that in vivo, GHRs are phosphorylated on tyrosyl residues only when GH is bound. cyclo(tyrosyl-tyrosyl) 56-63 growth hormone 1 Homo sapiens 29-31 1874195-1 1991 GH stimulates tyrosyl phosphorylation of GH receptors in 3T3-F442A fibroblasts, and highly purified GH receptor preparations exhibit tyrosine kinase activity. cyclo(tyrosyl-tyrosyl) 14-21 growth hormone 1 Homo sapiens 41-43 1874195-1 1991 GH stimulates tyrosyl phosphorylation of GH receptors in 3T3-F442A fibroblasts, and highly purified GH receptor preparations exhibit tyrosine kinase activity. cyclo(tyrosyl-tyrosyl) 14-21 growth hormone 1 Homo sapiens 0-2