Pub. Date : 1987 Feb 5
PMID : 3805049
6 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | cDNA clones for the P-450(M-1) mRNA, which exhibits a male-specific expression in rat livers, were isolated by using synthetic oligonucleotides as the probes. | Oligonucleotides | cytochrome P450, subfamily 2, polypeptide 11 | Rattus norvegicus |
| 2 | Sequence analysis of the cDNAs showed that P-450(M-1) mRNA contains 1,853 nucleotides in addition to a poly(A) chain, and a single open reading frame of 1,500 nucleotides encodes a polypeptide of 500 amino acids with a Mr = 57,187. | Poly A | cytochrome P450, subfamily 2, polypeptide 11 | Rattus norvegicus |
| 3 | The predicted NH2-terminal sequence of 30 amino acids agrees well with that of the purified protein determined by Edman degradation, and the predicted primary structure included all the partial sequences of six internal peptides of P-450(M-1) obtained by the proteolytic digestion and a conserved amino acid sequence containing a putative heme-binding cysteine, proximate to the COOH terminus of the molecules. | Heme | cytochrome P450, subfamily 2, polypeptide 11 | Rattus norvegicus |
| 4 | The predicted NH2-terminal sequence of 30 amino acids agrees well with that of the purified protein determined by Edman degradation, and the predicted primary structure included all the partial sequences of six internal peptides of P-450(M-1) obtained by the proteolytic digestion and a conserved amino acid sequence containing a putative heme-binding cysteine, proximate to the COOH terminus of the molecules. | Cysteine | cytochrome P450, subfamily 2, polypeptide 11 | Rattus norvegicus |
| 5 | Consequently, P-450(M-1) could be structurally classified into the phenobarbital-inducible type of P-450 gene family. | Phenobarbital | cytochrome P450, subfamily 2, polypeptide 11 | Rattus norvegicus |
| 6 | RNA blot analysis using a synthetic oligonucleotide specific for P-450(M-1) revealed that P-450(M-1) mRNA was expressed exclusively in the livers of mature male rats in a sex-specific manner, but not in other tissues so far examined. | Oligonucleotides | cytochrome P450, subfamily 2, polypeptide 11 | Rattus norvegicus |