Title : Spectroscopic and MD simulation studies on unfolding processes of mitochondrial carbonic anhydrase VA induced by urea.

Pub. Date : 2016 Sep

PMID : 26421381






3 Functional Relationships(s)
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1 Spectroscopic and MD simulation studies on unfolding processes of mitochondrial carbonic anhydrase VA induced by urea. Urea carbonic anhydrase 5A Homo sapiens
2 To understand the biophysical properties of CAVA, we carried out a reversible urea-induced isothermal denaturation at pH 7.0 and 25 C. Spectroscopic probes, [theta]222 (mean residue ellipticity at 222 nm), F344 (Trp-fluorescence emission intensity at 344 nm) and Deltaepsilon280 (difference absorption at 280 nm) were used to monitor the effect of urea on the structure and stability of CAVA. Urea carbonic anhydrase 5A Homo sapiens
3 We further performed 40 ns molecular dynamics simulation of CAVA to see the dynamics at different urea concentrations. Urea carbonic anhydrase 5A Homo sapiens