Pub. Date : 2016 Sep
PMID : 26421381
3 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
1 | Spectroscopic and MD simulation studies on unfolding processes of mitochondrial carbonic anhydrase VA induced by urea. | Urea | carbonic anhydrase 5A | Homo sapiens |
2 | To understand the biophysical properties of CAVA, we carried out a reversible urea-induced isothermal denaturation at pH 7.0 and 25 C. Spectroscopic probes, [theta]222 (mean residue ellipticity at 222 nm), F344 (Trp-fluorescence emission intensity at 344 nm) and Deltaepsilon280 (difference absorption at 280 nm) were used to monitor the effect of urea on the structure and stability of CAVA. | Urea | carbonic anhydrase 5A | Homo sapiens |
3 | We further performed 40 ns molecular dynamics simulation of CAVA to see the dynamics at different urea concentrations. | Urea | carbonic anhydrase 5A | Homo sapiens |