Title : Functional reconstitution and characterization of the Arabidopsis Mg(2+) transporter AtMRS2-10 in proteoliposomes.

Pub. Date : 2012 Sep

PMID : 22560897






10 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 Some members of the AtMRS2 family, including AtMRS2-10, may complement bacterial mutants or yeast mutants that lack Mg(2+) transport capabilities. magnesium ion magnesium transporter 1 Arabidopsis thaliana
2 AtMRS2-10, which contains an N-terminal His-tag, was expressed in Escherichia coli and solubilized with sarcosyl. Histidine magnesium transporter 1 Arabidopsis thaliana
3 Direct measurement of Mg(2+) uptake into proteoliposomes revealed that reconstituted AtMRS2-10 transported Mg(2+) without any accessory proteins. magnesium ion magnesium transporter 1 Arabidopsis thaliana
4 Direct measurement of Mg(2+) uptake into proteoliposomes revealed that reconstituted AtMRS2-10 transported Mg(2+) without any accessory proteins. magnesium ion magnesium transporter 1 Arabidopsis thaliana
5 The AtMRS2-10-mediated Mg(2+) influx was blocked by Co(III)hexamine, and was independent of the external pH from 5 to 9. magnesium ion magnesium transporter 1 Arabidopsis thaliana
6 The AtMRS2-10-mediated Mg(2+) influx was blocked by Co(III)hexamine, and was independent of the external pH from 5 to 9. co(iii)hexamine magnesium transporter 1 Arabidopsis thaliana
7 The activity of AtMRS2-10 was inhibited by Co(2+) and Ni(2+); however, it was not inhibited by Ca(2+), Fe(2+), or Fe(3+). Cobalt(2+) magnesium transporter 1 Arabidopsis thaliana
8 The activity of AtMRS2-10 was inhibited by Co(2+) and Ni(2+); however, it was not inhibited by Ca(2+), Fe(2+), or Fe(3+). Nickel(2+) magnesium transporter 1 Arabidopsis thaliana
9 While these results indicate that AtMRS2-10 has similar properties to the bacterial CorA proteins, unlike bacterial CorA proteins, AtMRS2-10 was potently inhibited by Al(3+). Aluminum magnesium transporter 1 Arabidopsis thaliana
10 While these results indicate that AtMRS2-10 has similar properties to the bacterial CorA proteins, unlike bacterial CorA proteins, AtMRS2-10 was potently inhibited by Al(3+). Aluminum magnesium transporter 1 Arabidopsis thaliana