Pub. Date : 2012 Jun 1
PMID : 22397410
9 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Golgi-SNARE GS28 potentiates cisplatin-induced apoptosis by forming GS28-MDM2-p53 complexes and by preventing the ubiquitination and degradation of p53. | Cisplatin | golgi SNAP receptor complex member 1 | Homo sapiens |
| 2 | Golgi-SNARE GS28 potentiates cisplatin-induced apoptosis by forming GS28-MDM2-p53 complexes and by preventing the ubiquitination and degradation of p53. | Cisplatin | golgi SNAP receptor complex member 1 | Homo sapiens |
| 3 | In the present study, we observed that the Golgi-SNARE (soluble N-ethylmaleimide-sensitive fusion protein-attachment protein receptor) GS28 forms a complex with p53 in HEK (human embryonic kidney)-293 cells. | Ethylmaleimide | golgi SNAP receptor complex member 1 | Homo sapiens |
| 4 | Given that p53 represents a tumour suppressor that affects the sensitivity of cancer cells to various chemotherapeutic drugs, we examined whether GS28 may influence the level of sensitivity to the DNA-damaging drug cisplatin. | Cisplatin | golgi SNAP receptor complex member 1 | Homo sapiens |
| 5 | Indeed, knockdown of GS28 using short-hairpin RNA (shGS28) induced resistance to cisplatin in HEK-293 cells. | Cisplatin | golgi SNAP receptor complex member 1 | Homo sapiens |
| 6 | On the other hand, overexpression of GS28 sensitized HEK-293 cells to cisplatin, whereas no sensitization effect was noted for the mitotic spindle-damaging drugs vincristine and taxol. | Cisplatin | golgi SNAP receptor complex member 1 | Homo sapiens |
| 7 | Conversely, GS28 overexpression induced the accumulation of p53 and Bax as well as the pro-apoptotic phosphorylation of p53 on Ser(46). | Serine | golgi SNAP receptor complex member 1 | Homo sapiens |
| 8 | The modulatory effects of GS28 on cisplatin sensitivity and p53 stability were absent in lung cancer H1299 cells which are p53-null. | Cisplatin | golgi SNAP receptor complex member 1 | Homo sapiens |
| 9 | As expected, ectopic expression of p53 in H1299 cells restored the modulatory effects of GS28 on sensitivity to cisplatin. | Cisplatin | golgi SNAP receptor complex member 1 | Homo sapiens |