Title : Improvement of ethanol yield from glycerol via conversion of pyruvate to ethanol in metabolically engineered Saccharomyces cerevisiae.

Pub. Date : 2012 Feb

PMID : 22161213






5 Functional Relationships(s)
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1 The overexpression of an alcohol dehydrogenase (adh1) and a pyruvate decarboxylase (pdc1) caused an increase in growth rate and glycerol consumption under fermentative conditions, which led to a slight increase of the final ethanol yield. Glycerol indolepyruvate decarboxylase 1 Saccharomyces cerevisiae S288C
2 The overexpression of an alcohol dehydrogenase (adh1) and a pyruvate decarboxylase (pdc1) caused an increase in growth rate and glycerol consumption under fermentative conditions, which led to a slight increase of the final ethanol yield. Ethanol indolepyruvate decarboxylase 1 Saccharomyces cerevisiae S288C
3 The overall expression of the adh1 and pdc1 genes in the modified strains, combined with the lack of the fps1 and gpd2 genes, resulted in a 1.4-fold increase (about 5.4 g/L ethanol produced) in fps1Deltagpd2Delta (pGcyaDak, pGupCas) (about 4.0 g/L ethanol produced). Ethanol indolepyruvate decarboxylase 1 Saccharomyces cerevisiae S288C
4 The overall expression of the adh1 and pdc1 genes in the modified strains, combined with the lack of the fps1 and gpd2 genes, resulted in a 1.4-fold increase (about 5.4 g/L ethanol produced) in fps1Deltagpd2Delta (pGcyaDak, pGupCas) (about 4.0 g/L ethanol produced). pgupcas indolepyruvate decarboxylase 1 Saccharomyces cerevisiae S288C
5 The overall expression of the adh1 and pdc1 genes in the modified strains, combined with the lack of the fps1 and gpd2 genes, resulted in a 1.4-fold increase (about 5.4 g/L ethanol produced) in fps1Deltagpd2Delta (pGcyaDak, pGupCas) (about 4.0 g/L ethanol produced). Ethanol indolepyruvate decarboxylase 1 Saccharomyces cerevisiae S288C