Title : Cholesterol dependent downregulation of mouse and human apical sodium dependent bile acid transporter (ASBT) gene expression: molecular mechanism and physiological consequences.

Pub. Date : 2006 Sep

PMID : 16484503






10 Functional Relationships(s)
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1 RESULTS: In mice, cholesterol enriched diet triggered a downregulation of ASBT expression (mRNA and protein), a fall in ileal bile acid uptake, and a rise in the faecal excretion of bile acids. Cholesterol solute carrier family 10, member 2 Mus musculus
2 Synthesised from cholesterol in the liver, bile acids are actively reclaimed in the ileum by the apical sodium dependent bile acid transporter (ASBT). Cholesterol solute carrier family 10, member 2 Mus musculus
3 Synthesised from cholesterol in the liver, bile acids are actively reclaimed in the ileum by the apical sodium dependent bile acid transporter (ASBT). Cholesterol solute carrier family 10, member 2 Mus musculus
4 Synthesised from cholesterol in the liver, bile acids are actively reclaimed in the ileum by the apical sodium dependent bile acid transporter (ASBT). Bile Acids and Salts solute carrier family 10, member 2 Mus musculus
5 Synthesised from cholesterol in the liver, bile acids are actively reclaimed in the ileum by the apical sodium dependent bile acid transporter (ASBT). Bile Acids and Salts solute carrier family 10, member 2 Mus musculus
6 Although the expression level of ASBT affects body cholesterol balance, the impact of cholesterol on ASBT gene expression remains unclear. Cholesterol solute carrier family 10, member 2 Mus musculus
7 Although the expression level of ASBT affects body cholesterol balance, the impact of cholesterol on ASBT gene expression remains unclear. Cholesterol solute carrier family 10, member 2 Mus musculus
8 In this study, the effect of cholesterol on ASBT expression and ileal bile acid uptake was explored in vivo and in vitro. Cholesterol solute carrier family 10, member 2 Mus musculus
9 METHODS: ASBT gene expression was assessed by real time quantitative polymerase chain reaction and northern or western blotting, or both, in mice subjected to a 2% cholesterol diet for two weeks, in mouse ileal explants, or in human enterocyte-like Caco-2 cells cultured in sterol enriched or depleted media. Cholesterol solute carrier family 10, member 2 Mus musculus
10 METHODS: ASBT gene expression was assessed by real time quantitative polymerase chain reaction and northern or western blotting, or both, in mice subjected to a 2% cholesterol diet for two weeks, in mouse ileal explants, or in human enterocyte-like Caco-2 cells cultured in sterol enriched or depleted media. Sterols solute carrier family 10, member 2 Mus musculus