Pub. Date : 1999 Jan 15
PMID : 9878443
3 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Here we report the results of an experiment in which we have hypermutated the bacterial enzyme TEM-1 beta-lactamase and selected small pools (<1.5x10(5)) of clones for enzymatic activity against the beta-lactam antibiotic cefotaxime. | Cefotaxime | CD248 molecule | Homo sapiens |
| 2 | The experiment resulted in the isolation of a number of TEM-1 mutants with greatly improved activity against cefotaxime. | Cefotaxime | CD248 molecule | Homo sapiens |
| 3 | Among these, clone 3D.5 (E104K:M182T:G238S) exhibited a minimum inhibitory concentration for cefotaxime 20,000-fold higher than wild-type TEM-1 and a catalytic efficiency (kcat/Km) 2383 times higher than the wild-type enzyme. | Cefotaxime | CD248 molecule | Homo sapiens |