Title : 25-hydroxylation of C27-steroids and vitamin D3 by a constitutive cytochrome P-450 from rat liver microsomes.

Pub. Date : 1983 Jun 10

PMID : 6304056






5 Functional Relationships(s)
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1 25-hydroxylation of C27-steroids and vitamin D3 by a constitutive cytochrome P-450 from rat liver microsomes. Cholecalciferol cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus
2 A constitutive cytochrome P-450 catalyzing 25-hydroxylation of C27-steroids and vitamin D3 was purified from rat liver microsomes. Cholecalciferol cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus
3 The purified cytochrome P-450 catalyzed 25-hydroxylation of 5 beta-cholestane-3 alpha, 7 alpha-diol, 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha-triol, and 1 alpha-hydroxyvitamin D3 up to 50 times more efficiently, and 25-hydroxylation of vitamin D3 about 150 times more efficiently than the microsomes. Cholecalciferol cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus
4 The 25-hydroxylation of 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha-triol and vitamin D3 with the purified cytochrome P-450 was not stimulated by addition of phospholipid or cytochrome b5 to the reconstituted system. Cholecalciferol cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus
5 The possibility that 25-hydroxylation of C27-steroids and vitamin D3 is catalyzed by the same species of cytochrome P-450 is discussed. Cholecalciferol cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus