Title : Non-enzymatic glycation of antithrombin III in vitro.

Pub. Date : 1988 Mar 17

PMID : 3349100






3 Functional Relationships(s)
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1 AT-III, isolated from normal human plasma by means of heparin affinity and ion-exchange chromatography, was incubated with 0-0.5 M glucose in neutral phosphate buffer at 37 degrees C. The extent of non-enzymatic glycation could be monitored by uptake of radioactivity as well as by binding to a phenylboronate affinity resin, which effectively retards AT-III containing ketoamine-linked glucose. Glucose serpin family C member 1 Homo sapiens
2 Following incubation with [14C]glucose, structural analyses of cyanogen-bromide-cleaved peptides of enzymatically glycated AT-III showed that the [14C]glucose adducts were distributed over many sites on the molecule. Glucose serpin family C member 1 Homo sapiens
3 Following incubation with [14C]glucose, structural analyses of cyanogen-bromide-cleaved peptides of enzymatically glycated AT-III showed that the [14C]glucose adducts were distributed over many sites on the molecule. Glucose serpin family C member 1 Homo sapiens