Title : Fluorescent detection of PARP activity in unfixed tissue.

Pub. Date : 2021

PMID : 33481867






2 Functional Relationships(s)
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1 A previously published two-step PARP activity assay uses biotinylated NAD+ and streptavidin labelling for this purpose. NAD poly(ADP-ribose) polymerase 1 Homo sapiens
2 Here, we used the fluorescent NAD+ analogues epsilon-NAD+ and 6-Fluo-10-NAD+ to assess PARP activity directly on unfixed tissue sections obtained from wild-type and retinal degeneration-1 (rd1) mutant retina. NAD poly(ADP-ribose) polymerase 1 Homo sapiens