Title : Nicotine Upregulates the Level of Mcl-1 through STAT3 in H1299 Cells.

Pub. Date : 2020

PMID : 31956373






6 Functional Relationships(s)
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1 Nicotine Upregulates the Level of Mcl-1 through STAT3 in H1299 Cells. Nicotine signal transducer and activator of transcription 3 Homo sapiens
2 Therefore, we examined the STAT3 cascade in nicotine regulation of Mcl-1 transcription in human lung cancer cells. Nicotine signal transducer and activator of transcription 3 Homo sapiens
3 Methods: The effects of nicotine on the expression of STAT3 and Mcl-1 were determined using western blot. Nicotine signal transducer and activator of transcription 3 Homo sapiens
4 Results: STAT3 was constitutively activated (i.e., tyrosine-phosphorylated, serine-phosphorylated and nuclear translocation), meanwhile the expression and transcriptional activity of Mcl-1 were up-regulated in lung cancer cells following treatment with nicotine. Nicotine signal transducer and activator of transcription 3 Homo sapiens
5 Deleted mutagenesis of a putative STAT3 consensus binding sequence decreased Mcl-1 promoter activity and eliminated the increase of Mcl-1 promoter activity induced by nicotine. Nicotine signal transducer and activator of transcription 3 Homo sapiens
6 Conclusions: We have demonstrated that nicotine induces up-regulation of Mcl-1 through STAT3, which process may be independent on JAKs and not only dependent on the phosphorylation of Y705. Nicotine signal transducer and activator of transcription 3 Homo sapiens