Title : Thyroid hormones modulate thyrotropin-releasing hormone biosynthesis in tissues outside the hypothalamic-pituitary axis of male rats.

Pub. Date : 1989 Jul

PMID : 2500333






15 Functional Relationships(s)
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1 Within secretory granules, TRH-Gly is converted to TRH through alpha-amidation of the C-terminal proline residue, using Gly as the NH2 donor. Glycine thyrotropin releasing hormone Rattus norvegicus
2 Using specific RIA, we measured the TRH-Gly immunoreactivity (TRH-Gly-IR) and TRH-IR concentrations in tissues from the reproductive and gastrointestinal systems, adrenals, and other internal organs in euthyroid, hypothyroid, and T4-treated 250-g Sprague-Dawley male rats. Glycine thyrotropin releasing hormone Rattus norvegicus
3 Using specific RIA, we measured the TRH-Gly immunoreactivity (TRH-Gly-IR) and TRH-IR concentrations in tissues from the reproductive and gastrointestinal systems, adrenals, and other internal organs in euthyroid, hypothyroid, and T4-treated 250-g Sprague-Dawley male rats. Glycine thyrotropin releasing hormone Rattus norvegicus
4 Using specific RIA, we measured the TRH-Gly immunoreactivity (TRH-Gly-IR) and TRH-IR concentrations in tissues from the reproductive and gastrointestinal systems, adrenals, and other internal organs in euthyroid, hypothyroid, and T4-treated 250-g Sprague-Dawley male rats. Glycine thyrotropin releasing hormone Rattus norvegicus
5 TRH-Gly-IR concentrations were more than 2-fold higher than TRH-IR concentrations within the adrenal, pancreas, bowel, and stomach at the time of death. Glycine thyrotropin releasing hormone Rattus norvegicus
6 Untreated hypothyroidism and exogenous TRH significantly increased adrenal TRH-Gly-IR levels. Glycine thyrotropin releasing hormone Rattus norvegicus
7 Untreated hypothyroidism and exogenous TRH significantly increased adrenal TRH-Gly-IR levels. Glycine thyrotropin releasing hormone Rattus norvegicus
8 Pancreatic TRH-Gly levels increased about 2-fold in hypothyroid rats. Glycine thyrotropin releasing hormone Rattus norvegicus
9 Incubation at 60 C significantly increased TRH-Gly-IR levels in the pancreas, adrenal, bowel, stomach, and epididymis by 14-, 3-, 6-, 6-, and 6-fold, respectively. Glycine thyrotropin releasing hormone Rattus norvegicus
10 Also after 60 C incubation increases in the TRH-Gly-IR/TRH-IR ratio of 2.7-, 4-, and 1.7-fold were observed in the pancreas, epididymis, and bowel, respectively. Glycine thyrotropin releasing hormone Rattus norvegicus
11 Pooled tissue extracts were fractionated by cation exchange and reverse phase HPLC for characterization of TRH-Gly-IR. Glycine thyrotropin releasing hormone Rattus norvegicus
12 Both chromatographic methods revealed a major peak of TRH-Gly-IR coeluting with synthetic TRH-Gly. Glycine thyrotropin releasing hormone Rattus norvegicus
13 Incubation at 60 C caused 13.5-, 4.1-, 1.5-, and 5-fold increments in the TRH-Gly-IR for adrenal, pancreas, prostate, and thyroid, respectively, compared to the immediately extracted control aliquots. Glycine thyrotropin releasing hormone Rattus norvegicus
14 Cation exchange and reverse phase HPLC also revealed production of higher mol wt TRH precursor peptides after incubation at 60 C for 4 or 20 h. Only the TRH-Gly-IR peak coeluting with pGlu-His-Pro-Gly was converted into TRH by rat brain alpha-amidating enzyme. Glycine thyrotropin releasing hormone Rattus norvegicus
15 Cation exchange and reverse phase HPLC also revealed production of higher mol wt TRH precursor peptides after incubation at 60 C for 4 or 20 h. Only the TRH-Gly-IR peak coeluting with pGlu-His-Pro-Gly was converted into TRH by rat brain alpha-amidating enzyme. Glycine thyrotropin releasing hormone Rattus norvegicus