Title : Galloflavin prevents the binding of lactate dehydrogenase A to single stranded DNA and inhibits RNA synthesis in cultured cells.

Pub. Date : 2013 Jan 11

PMID : 23237800






3 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 Binding is prevented by NADH, suggesting that the coenzyme site is involved in the interaction LDH-A/ssDNA. NAD lactate dehydrogenase A Homo sapiens
2 We recently identified an inhibitor of LDH-A enzymatic activity (Galloflavin, GF) which occupies the NADH site. NAD lactate dehydrogenase A Homo sapiens
3 Our results suggest that: (i) inhibitors which bind the NADH site can exert their antiproliferative activity not only by blocking aerobic glycolysis but also by causing an inhibition of RNA synthesis independent from the effect on glycolysis; (ii) GF can be a useful tool to study the biological role of LDH-A binding to ssDNA. NAD lactate dehydrogenase A Homo sapiens