Title : Detection of heme oxygenase activity by measurement of CO.

Pub. Date : 2001 May

PMID : 23045068






1 Functional Relationships(s)
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1 Detection of heme oxygenase activity by measurement of CO. Heme oxygenase (HO) is the first and rate-limiting step in degradation of heme, and in the presence of NADPH-cytochrome P-450 reductase it produces equimolar amounts of biliverdin and CO. CO produced in a closed system can be quantified as described in this unit by gas chromatography as a measure of HO activity in tissue slices, tissue homogenates, and tissue fractions. Biliverdine cytochrome p450 oxidoreductase Homo sapiens