Title : Tumor necrosis factor alpha 'primes' the platelet-activating factor-induced superoxide production by human neutrophils: possible involvement of G proteins.

Pub. Date : 1990

PMID : 1966810






7 Functional Relationships(s)
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1 Tumor necrosis factor alpha "primes" the platelet-activating factor-induced superoxide production by human neutrophils: possible involvement of G proteins. Superoxides PCNA clamp associated factor Homo sapiens
2 We were interested to ascertain whether superoxide generation by human PMN could be amplified by PAF following initial stimulation with tumor necrosis factor (TNF) and the effect of cholera and pertussis toxin on this process. Superoxides PCNA clamp associated factor Homo sapiens
3 PAF alone (0.1 pM-0.1 nM) failed to evoke any superoxide production; however, when PAF was added for 10 min to cells previously incubated for 50 min with 10 ng/ml TNF, superoxide production was significantly enhanced relative to that induced by TNF alone. Superoxides PCNA clamp associated factor Homo sapiens
4 The PAF antagonists also decreased by 25% the superoxide production elicited solely by TNF, indicating that TNF-induced superoxide generation is partially mediated by a mechanism involving endogenous PAF. Superoxides PCNA clamp associated factor Homo sapiens
5 The PAF antagonists also decreased by 25% the superoxide production elicited solely by TNF, indicating that TNF-induced superoxide generation is partially mediated by a mechanism involving endogenous PAF. Superoxides PCNA clamp associated factor Homo sapiens
6 The PAF antagonists also decreased by 25% the superoxide production elicited solely by TNF, indicating that TNF-induced superoxide generation is partially mediated by a mechanism involving endogenous PAF. Superoxides PCNA clamp associated factor Homo sapiens
7 Pretreatment of the PMN with pertussis or cholera toxin reduced the amplification of superoxide production induced by PAF in TNF-stimulated PMN, implicating pertussis and cholera toxin-sensitive G-proteins in the amplification process. Superoxides PCNA clamp associated factor Homo sapiens