Title : Stimulation of Akt poly-ubiquitination and proteasomal degradation in P388D1 cells by 7-ketocholesterol and 25-hydroxycholesterol.

Pub. Date : 2009 Jul 1

PMID : 19464253






5 Functional Relationships(s)
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1 Stimulation of Akt poly-ubiquitination and proteasomal degradation in P388D1 cells by 7-ketocholesterol and 25-hydroxycholesterol. 25-hydroxycholesterol thymoma viral proto-oncogene 1 Mus musculus
2 Previously we observed enhanced degradation of Akt in P388D1 moncocyte/macrophages following treatment with 25-hydroxycholesterol (25-OH) or 7-ketocholesterol (7-KC). 25-hydroxycholesterol thymoma viral proto-oncogene 1 Mus musculus
3 Previously we observed enhanced degradation of Akt in P388D1 moncocyte/macrophages following treatment with 25-hydroxycholesterol (25-OH) or 7-ketocholesterol (7-KC). 25-hydroxycholesterol thymoma viral proto-oncogene 1 Mus musculus
4 We show that treatment with 25-OH or 7-KC results in the accumulation of poly-ubiquitinated Akt, an effect that is enhanced by co-treatment with the proteasome inhibitor MG-132. 25-hydroxycholesterol thymoma viral proto-oncogene 1 Mus musculus
5 Modification of Akt by the addition of a Gly-Ala repeat (GAr), a domain known to block ubiquitin-dependent targeting of proteins to the proteasome, resulted in a chimeric protein that is resistant to turn-over induced by 25-OH or 7-KC and provides protection from apoptosis induced by these oxysterols. 25-hydroxycholesterol thymoma viral proto-oncogene 1 Mus musculus