Title : The measurement of cyclic nucleotide phosphodiesterase 4 activities via the quantification of inorganic phosphate with malachite green.

Pub. Date : 2009 Mar 16

PMID : 19231363






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1 A spectrometric method was investigated to measure the activities of recombinant human cyclic nucleotide phosphodiesterase 4 (PDE4), based on the use of malachite green (MLG) to quantify phosphate released from adenosine-5"-monophosphate (AMP) by the action of calf intestinal alkaline phosphatase (CIAP). adenosine-5"-monophosphate phosphodiesterase 4A Homo sapiens