Pub. Date : 2008 Apr 2
PMID : 18331839
3 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | We show that thrombin can be used for the formation of N-terminal Cys, providing another choice of reagents for expressed protein ligation. | Cysteine | coagulation factor II, thrombin | Homo sapiens |
| 2 | Proteins with N-terminal Cys can be obtained by the convenient modification of vectors with the putative thrombin cleavage site LVPRG to LVPRC. | Cysteine | coagulation factor II, thrombin | Homo sapiens |
| 3 | The use of thrombin protease to generate N-terminal Cys overcomes some of the limitations of existing methods, making it generally useful for expressed protein ligation and other biotechnological applications. | Cysteine | coagulation factor II, thrombin | Homo sapiens |