PMID-sentid Pub_year Sent_text comp_official_name comp_offset protein_name organism prot_offset 2689861-1 1989 The enzymes of the proline utilization pathway (the products of the PUT1 and PUT2 genes) in Saccharomyces cerevisiae are coordinately regulated by proline and the PUT3 transcriptional activator. Proline 19-26 proline dehydrogenase Saccharomyces cerevisiae S288C 68-72 34442729-3 2021 Here, we discovered that proline oxidation, catalyzed by the proline oxidase Put1, a mitochondrial flavin-dependent enzyme converting proline into 1-pyrroline-5-carboxylate, controls the chronological lifespan of the yeast Saccharomyces cerevisiae. Proline 25-32 proline dehydrogenase Saccharomyces cerevisiae S288C 77-81 34442729-3 2021 Here, we discovered that proline oxidation, catalyzed by the proline oxidase Put1, a mitochondrial flavin-dependent enzyme converting proline into 1-pyrroline-5-carboxylate, controls the chronological lifespan of the yeast Saccharomyces cerevisiae. 4,6-dinitro-o-cresol 99-105 proline dehydrogenase Saccharomyces cerevisiae S288C 77-81 34442729-3 2021 Here, we discovered that proline oxidation, catalyzed by the proline oxidase Put1, a mitochondrial flavin-dependent enzyme converting proline into 1-pyrroline-5-carboxylate, controls the chronological lifespan of the yeast Saccharomyces cerevisiae. Proline 134-141 proline dehydrogenase Saccharomyces cerevisiae S288C 77-81 34442729-3 2021 Here, we discovered that proline oxidation, catalyzed by the proline oxidase Put1, a mitochondrial flavin-dependent enzyme converting proline into 1-pyrroline-5-carboxylate, controls the chronological lifespan of the yeast Saccharomyces cerevisiae. delta-1-pyrroline-5-carboxylate 148-173 proline dehydrogenase Saccharomyces cerevisiae S288C 77-81 34442729-8 2021 More importantly, the oxidation of proline by Put1 helped maintain the mitochondrial membrane potential and ATP production through the aging period. Proline 35-42 proline dehydrogenase Saccharomyces cerevisiae S288C 46-50 34442729-8 2021 More importantly, the oxidation of proline by Put1 helped maintain the mitochondrial membrane potential and ATP production through the aging period. Adenosine Triphosphate 108-111 proline dehydrogenase Saccharomyces cerevisiae S288C 46-50 2689861-1 1989 The enzymes of the proline utilization pathway (the products of the PUT1 and PUT2 genes) in Saccharomyces cerevisiae are coordinately regulated by proline and the PUT3 transcriptional activator. Proline 147-154 proline dehydrogenase Saccharomyces cerevisiae S288C 68-72 23894476-9 2013 In addition, when Saccharomyces cerevisiae null mutants for this gene (PUT1) were complemented with the TcPRODH gene, diminished free intracellular proline levels and an enhanced sensitivity to oxidative stress in comparison to the null mutant were observed, supporting the hypothesis that free proline accumulation constitutes a defense against oxidative imbalance. Proline 148-155 proline dehydrogenase Saccharomyces cerevisiae S288C 71-75 3121434-1 1987 A mutation has been identified that prevents Saccharomyces cerevisiae cells from growing on proline as the sole source of nitrogen, causes noninducible expression of the PUT1 and PUT2 genes, and is completely recessive. Proline 92-99 proline dehydrogenase Saccharomyces cerevisiae S288C 170-174 3121434-1 1987 A mutation has been identified that prevents Saccharomyces cerevisiae cells from growing on proline as the sole source of nitrogen, causes noninducible expression of the PUT1 and PUT2 genes, and is completely recessive. Nitrogen 122-130 proline dehydrogenase Saccharomyces cerevisiae S288C 170-174 31646149-4 2019 Deletion of the proline oxidase gene PUT1 and expression of the gamma-glutamate kinase mutant gene PRO1-I150T that is less sensitive to feedback inhibition accumulated proline and extended the replicative lifespan of yeast cells. isoleucyl-prolyl-proline 16-23 proline dehydrogenase Saccharomyces cerevisiae S288C 37-41 31646149-7 2019 Overexpression of the stress-responsive transcription activator gene MSN2 reduced intracellular proline levels by inducing the expression of PUT1, resulting in a short lifespan. isoleucyl-prolyl-proline 96-103 proline dehydrogenase Saccharomyces cerevisiae S288C 141-145 29607284-5 2018 When tested for deletion of the S. cerevisiae proline oxidase gene PUT1, the hphEC6 cassette was shown to successfully act as a selection marker on hygromycin B-containing medium. Hygromycin B 148-160 proline dehydrogenase Saccharomyces cerevisiae S288C 67-71 26965428-7 2016 Moreover, mutant with simultaneous deletion of NTH1 and PUT1 exhibits the highest relative dough-leavening ability after freezing compared to mutants with single-gene deletion perhaps due to elevated levels of both trehalose and proline. Trehalose 215-224 proline dehydrogenase Saccharomyces cerevisiae S288C 56-60 26965428-7 2016 Moreover, mutant with simultaneous deletion of NTH1 and PUT1 exhibits the highest relative dough-leavening ability after freezing compared to mutants with single-gene deletion perhaps due to elevated levels of both trehalose and proline. Proline 229-236 proline dehydrogenase Saccharomyces cerevisiae S288C 56-60 23824663-2 2014 Proline-fueled mitochondrial metabolism involves the oxidative conversion of L-Proline to L-Glutamate in two enzymatic steps by means of Put1p and Put2p that help Saccharomyces cerevisiae to respond to changes in the nutritional environment by initiating the breakdown of L-Proline as a source for nitrogen, carbon, and energy. Proline 0-7 proline dehydrogenase Saccharomyces cerevisiae S288C 137-142 23824663-2 2014 Proline-fueled mitochondrial metabolism involves the oxidative conversion of L-Proline to L-Glutamate in two enzymatic steps by means of Put1p and Put2p that help Saccharomyces cerevisiae to respond to changes in the nutritional environment by initiating the breakdown of L-Proline as a source for nitrogen, carbon, and energy. Proline 77-86 proline dehydrogenase Saccharomyces cerevisiae S288C 137-142 23824663-2 2014 Proline-fueled mitochondrial metabolism involves the oxidative conversion of L-Proline to L-Glutamate in two enzymatic steps by means of Put1p and Put2p that help Saccharomyces cerevisiae to respond to changes in the nutritional environment by initiating the breakdown of L-Proline as a source for nitrogen, carbon, and energy. Glutamic Acid 90-101 proline dehydrogenase Saccharomyces cerevisiae S288C 137-142 23824663-2 2014 Proline-fueled mitochondrial metabolism involves the oxidative conversion of L-Proline to L-Glutamate in two enzymatic steps by means of Put1p and Put2p that help Saccharomyces cerevisiae to respond to changes in the nutritional environment by initiating the breakdown of L-Proline as a source for nitrogen, carbon, and energy. Proline 272-281 proline dehydrogenase Saccharomyces cerevisiae S288C 137-142 23824663-2 2014 Proline-fueled mitochondrial metabolism involves the oxidative conversion of L-Proline to L-Glutamate in two enzymatic steps by means of Put1p and Put2p that help Saccharomyces cerevisiae to respond to changes in the nutritional environment by initiating the breakdown of L-Proline as a source for nitrogen, carbon, and energy. Nitrogen 298-306 proline dehydrogenase Saccharomyces cerevisiae S288C 137-142 23824663-2 2014 Proline-fueled mitochondrial metabolism involves the oxidative conversion of L-Proline to L-Glutamate in two enzymatic steps by means of Put1p and Put2p that help Saccharomyces cerevisiae to respond to changes in the nutritional environment by initiating the breakdown of L-Proline as a source for nitrogen, carbon, and energy. Carbon 308-314 proline dehydrogenase Saccharomyces cerevisiae S288C 137-142 24039956-3 2013 Additionally, the C. parasitica Prodh and P5Cdh genes were able to complement the Saccharomyces cerevisiae put1 and put2 null mutants, respectively, to allow these proline auxotrophic yeast mutants to grow on media with proline as the sole source of nitrogen. Proline 164-171 proline dehydrogenase Saccharomyces cerevisiae S288C 107-111 24039956-3 2013 Additionally, the C. parasitica Prodh and P5Cdh genes were able to complement the Saccharomyces cerevisiae put1 and put2 null mutants, respectively, to allow these proline auxotrophic yeast mutants to grow on media with proline as the sole source of nitrogen. Proline 220-227 proline dehydrogenase Saccharomyces cerevisiae S288C 107-111 24039956-3 2013 Additionally, the C. parasitica Prodh and P5Cdh genes were able to complement the Saccharomyces cerevisiae put1 and put2 null mutants, respectively, to allow these proline auxotrophic yeast mutants to grow on media with proline as the sole source of nitrogen. Nitrogen 250-258 proline dehydrogenase Saccharomyces cerevisiae S288C 107-111 2689862-1 1989 The PUT1 and PUT2 genes encoding the enzymes of the proline utilization pathway of Saccharomyces cerevisiae are induced by proline and activated by the product of the PUT3 gene. Proline 52-59 proline dehydrogenase Saccharomyces cerevisiae S288C 4-8 2689862-1 1989 The PUT1 and PUT2 genes encoding the enzymes of the proline utilization pathway of Saccharomyces cerevisiae are induced by proline and activated by the product of the PUT3 gene. Proline 123-130 proline dehydrogenase Saccharomyces cerevisiae S288C 4-8 3125423-0 1987 Proline utilization in Saccharomyces cerevisiae: sequence, regulation, and mitochondrial localization of the PUT1 gene product. Proline 0-7 proline dehydrogenase Saccharomyces cerevisiae S288C 109-113 3125423-5 1987 PUT1 is inducible by proline, responds only slightly to carbon catabolite repression, and is not regulated by the cytochrome activator proteins HAP1 and HAP2. Proline 21-28 proline dehydrogenase Saccharomyces cerevisiae S288C 0-4 3125423-5 1987 PUT1 is inducible by proline, responds only slightly to carbon catabolite repression, and is not regulated by the cytochrome activator proteins HAP1 and HAP2. carbon catabolite 56-73 proline dehydrogenase Saccharomyces cerevisiae S288C 0-4 3125423-6 1987 The PUT1 gene is under oxygen regulation; expression in anaerobically grown cells is 10-fold lower than in aerobically grown cells. Oxygen 23-29 proline dehydrogenase Saccharomyces cerevisiae S288C 4-8 3125423-9 1987 Studies on PUT1 promoter deletions define a region between positions -458 and -293 from the translation initiation site that is important for full expression of the PUT1 gene and required for oxygen regulation. Oxygen 192-198 proline dehydrogenase Saccharomyces cerevisiae S288C 11-15 3121434-2 1987 In the put3-75 mutant, the basal level of expression (ammonia as nitrogen source) of PUT1-lacZ and PUT2-lacZ gene fusions as measured by beta-galactosidase activity is reduced 4- and 7-fold, respectively, compared with the wild-type strain. Ammonia 54-61 proline dehydrogenase Saccharomyces cerevisiae S288C 85-89 3121434-2 1987 In the put3-75 mutant, the basal level of expression (ammonia as nitrogen source) of PUT1-lacZ and PUT2-lacZ gene fusions as measured by beta-galactosidase activity is reduced 4- and 7-fold, respectively, compared with the wild-type strain. Nitrogen 65-73 proline dehydrogenase Saccharomyces cerevisiae S288C 85-89 3537723-0 1986 Proline utilization in Saccharomyces cerevisiae: analysis of the cloned PUT1 gene. Proline 0-7 proline dehydrogenase Saccharomyces cerevisiae S288C 72-76 3537723-8 1986 Approximately 50-fold more PUT1-specific mRNA was detected in induced (proline-grown) cells than in uninduced (ammonia-grown) cells. Proline 71-78 proline dehydrogenase Saccharomyces cerevisiae S288C 27-31 3537723-8 1986 Approximately 50-fold more PUT1-specific mRNA was detected in induced (proline-grown) cells than in uninduced (ammonia-grown) cells. Ammonia 111-118 proline dehydrogenase Saccharomyces cerevisiae S288C 27-31 29242833-1 2017 The zinc cluster transcription factor Put3 was initially characterized in Saccharomyces cerevisiae as the transcriptional activator of PUT1 and PUT2, two genes acting early in the proline assimilation pathway. Proline 180-187 proline dehydrogenase Saccharomyces cerevisiae S288C 135-139 26833688-5 2016 When cultured in YPD medium, there was a significant accumulation of proline in the put1 mutant strain, which is deficient in proline oxidase, in the stationary phase. ypd medium 17-27 proline dehydrogenase Saccharomyces cerevisiae S288C 84-88 26833688-5 2016 When cultured in YPD medium, there was a significant accumulation of proline in the put1 mutant strain, which is deficient in proline oxidase, in the stationary phase. Proline 69-76 proline dehydrogenase Saccharomyces cerevisiae S288C 84-88 26833688-6 2016 Expression of the mutant PRO1 gene, which encodes the gamma-glutamyl kinase variant (Asp154Asn or Ile150Thr) with desensitization to feedback inhibition by proline in the put1 mutant strain, showed a prominent increase in proline content as compared with that of the wild-type strain. Proline 156-163 proline dehydrogenase Saccharomyces cerevisiae S288C 171-175 26833688-6 2016 Expression of the mutant PRO1 gene, which encodes the gamma-glutamyl kinase variant (Asp154Asn or Ile150Thr) with desensitization to feedback inhibition by proline in the put1 mutant strain, showed a prominent increase in proline content as compared with that of the wild-type strain. Proline 222-229 proline dehydrogenase Saccharomyces cerevisiae S288C 171-175 24334254-8 2014 This is clearly illustrated by the differences in copy numbers not only in gene PUT1, the main player in the assimilation of proline as a nitrogen source, but also in CAR2, involved in arginine catabolism. Proline 125-132 proline dehydrogenase Saccharomyces cerevisiae S288C 80-84 24334254-8 2014 This is clearly illustrated by the differences in copy numbers not only in gene PUT1, the main player in the assimilation of proline as a nitrogen source, but also in CAR2, involved in arginine catabolism. Nitrogen 138-146 proline dehydrogenase Saccharomyces cerevisiae S288C 80-84 24334254-8 2014 This is clearly illustrated by the differences in copy numbers not only in gene PUT1, the main player in the assimilation of proline as a nitrogen source, but also in CAR2, involved in arginine catabolism. Arginine 185-193 proline dehydrogenase Saccharomyces cerevisiae S288C 80-84 24334254-9 2014 Strains isolated from fermentations where proline is more abundant contain a higher number of PUT1 copies and are more efficient in assimilating this amino acid as a nitrogen source. Proline 42-49 proline dehydrogenase Saccharomyces cerevisiae S288C 94-98 23894476-9 2013 In addition, when Saccharomyces cerevisiae null mutants for this gene (PUT1) were complemented with the TcPRODH gene, diminished free intracellular proline levels and an enhanced sensitivity to oxidative stress in comparison to the null mutant were observed, supporting the hypothesis that free proline accumulation constitutes a defense against oxidative imbalance. Proline 295-302 proline dehydrogenase Saccharomyces cerevisiae S288C 71-75 22462683-4 2012 Recently, we revealed a novel antioxidative mechanism in a laboratory yeast strain that is involved in stress-induced nitric oxide (NO) synthesis from proline via proline oxidase Put1 and N-acetyltransferase Mpr1. Nitric Oxide 118-130 proline dehydrogenase Saccharomyces cerevisiae S288C 179-183 22462683-4 2012 Recently, we revealed a novel antioxidative mechanism in a laboratory yeast strain that is involved in stress-induced nitric oxide (NO) synthesis from proline via proline oxidase Put1 and N-acetyltransferase Mpr1. Proline 151-158 proline dehydrogenase Saccharomyces cerevisiae S288C 179-183 22462683-12 2012 CONCLUSIONS: In this work, we clarified the importance of Put1- and Mpr1-mediated NO generation from proline to the baking-associated stress tolerance in industrial baker"s yeast. Proline 101-108 proline dehydrogenase Saccharomyces cerevisiae S288C 58-62 20550582-7 2010 Under oxidative stress conditions, the transcription of PUT1 encoding the proline oxidase Put1 and MPR1 was strongly induced, and consequently, the arginine content was significantly increased. Arginine 148-156 proline dehydrogenase Saccharomyces cerevisiae S288C 56-60 20550582-7 2010 Under oxidative stress conditions, the transcription of PUT1 encoding the proline oxidase Put1 and MPR1 was strongly induced, and consequently, the arginine content was significantly increased. Arginine 148-156 proline dehydrogenase Saccharomyces cerevisiae S288C 90-94 20550582-10 2010 We propose an antioxidative mechanism that is involved in stress-induced arginine synthesis requiring Mpr1 and Put1. Arginine 73-81 proline dehydrogenase Saccharomyces cerevisiae S288C 111-115 20450881-1 2010 In Saccharomyces cerevisiae, the PUT1 and PUT2 genes are required for the conversion of proline to glutamate. Proline 88-95 proline dehydrogenase Saccharomyces cerevisiae S288C 33-37 20450881-1 2010 In Saccharomyces cerevisiae, the PUT1 and PUT2 genes are required for the conversion of proline to glutamate. Glutamic Acid 99-108 proline dehydrogenase Saccharomyces cerevisiae S288C 33-37 20450881-3 2010 Put1p was expressed and purified from Escherichia coli and shown to have a UV-visible absorption spectrum that is typical of a bound flavin cofactor. 4,6-dinitro-o-cresol 133-139 proline dehydrogenase Saccharomyces cerevisiae S288C 0-5 20450881-4 2010 A K(m) value of 36 mM proline and a k(cat)=27 s(-1) were determined for Put1p using an artificial electron acceptor. Proline 22-29 proline dehydrogenase Saccharomyces cerevisiae S288C 72-77 20450881-5 2010 Put1p also exhibited high activity using ubiquinone-1 (CoQ(1)) as an electron acceptor with a k(cat)=9.6 s(-1) and a K(m) of 33 microM for CoQ(1). Ubiquinone Q1 41-53 proline dehydrogenase Saccharomyces cerevisiae S288C 0-5 19574222-2 2009 The transcriptional activator Put3p allows yeast cells to utilize proline as a nitrogen source through expression of the PUT1 and PUT2 genes. Proline 66-73 proline dehydrogenase Saccharomyces cerevisiae S288C 121-125 19574222-8 2009 Here, we find that the mutation of Put3p at amino acid Tyr-788 modulates the proline-independent activation of PUT1 through Gat1p. Tyrosine 55-58 proline dehydrogenase Saccharomyces cerevisiae S288C 111-115 19574222-8 2009 Here, we find that the mutation of Put3p at amino acid Tyr-788 modulates the proline-independent activation of PUT1 through Gat1p. Proline 77-84 proline dehydrogenase Saccharomyces cerevisiae S288C 111-115 19574222-2 2009 The transcriptional activator Put3p allows yeast cells to utilize proline as a nitrogen source through expression of the PUT1 and PUT2 genes. Nitrogen 79-87 proline dehydrogenase Saccharomyces cerevisiae S288C 121-125 18940788-4 2008 Frequently, this overexpression is mediated by the transcription factor Put3, which also regulates the proline catabolic genes PUT1 and PUT2. Proline 103-110 proline dehydrogenase Saccharomyces cerevisiae S288C 127-131 18940788-5 2008 The increased expression of MCH5 may increase the concentrations of FAD, which is the coenzyme required for the activity of proline oxidase, encoded by PUT1. Flavin-Adenine Dinucleotide 68-71 proline dehydrogenase Saccharomyces cerevisiae S288C 152-156 16233323-1 2002 In Saccharomyces cerevisiae, the PUT1-encoded proline oxidase and the PUT2-encoded delta1-pyrroline-5-carboxylate dehydrogenase are required to convert proline to glutamate. Proline 46-53 proline dehydrogenase Saccharomyces cerevisiae S288C 33-37 18802692-6 2008 Based on the fact that proline has stress-protective activity, S. cerevisiae cells that accumulate proline were constructed by disrupting the PUT1 gene involved in the degradation pathway and by expressing the mutant PRO1 gene encoding the feedback inhibition-less sensitive gamma-glutamate kinase to enhance the biosynthetic activity. Proline 23-30 proline dehydrogenase Saccharomyces cerevisiae S288C 142-146 18802692-6 2008 Based on the fact that proline has stress-protective activity, S. cerevisiae cells that accumulate proline were constructed by disrupting the PUT1 gene involved in the degradation pathway and by expressing the mutant PRO1 gene encoding the feedback inhibition-less sensitive gamma-glutamate kinase to enhance the biosynthetic activity. Proline 99-106 proline dehydrogenase Saccharomyces cerevisiae S288C 142-146 16751508-2 2006 We altered intracellular proline levels by overexpressing the proline dehydrogenase gene (PUT1) of S. cerevisiae. Proline 25-32 proline dehydrogenase Saccharomyces cerevisiae S288C 90-94 16751508-3 2006 Put1p performs the first enzymatic step of proline degradation in S. cerevisiae. Proline 43-50 proline dehydrogenase Saccharomyces cerevisiae S288C 0-5 16751508-4 2006 Overexpression of Put1p results in low proline levels and hypersensitivity to oxidants, such as hydrogen peroxide and paraquat. Proline 39-46 proline dehydrogenase Saccharomyces cerevisiae S288C 18-23 16751508-4 2006 Overexpression of Put1p results in low proline levels and hypersensitivity to oxidants, such as hydrogen peroxide and paraquat. Hydrogen Peroxide 96-113 proline dehydrogenase Saccharomyces cerevisiae S288C 18-23 16751508-4 2006 Overexpression of Put1p results in low proline levels and hypersensitivity to oxidants, such as hydrogen peroxide and paraquat. Paraquat 118-126 proline dehydrogenase Saccharomyces cerevisiae S288C 18-23 16751508-5 2006 A put1-disrupted yeast mutant deficient in Put1p activity has increased protection from oxidative stress and increased proline levels. Proline 119-126 proline dehydrogenase Saccharomyces cerevisiae S288C 2-6 16751508-5 2006 A put1-disrupted yeast mutant deficient in Put1p activity has increased protection from oxidative stress and increased proline levels. Proline 119-126 proline dehydrogenase Saccharomyces cerevisiae S288C 43-48 16751508-6 2006 Following a conditional life/death screen in yeast, we identified a tomato (Lycopersicon esculentum) gene encoding a QM-like protein (tQM) and found that stable expression of tQM in the Put1p-overexpressing strain conferred protection against oxidative damage from H2O2, paraquat, and heat. Hydrogen Peroxide 265-269 proline dehydrogenase Saccharomyces cerevisiae S288C 186-191 16751508-8 2006 A yeast two-hybrid system assay was used to show that tQM physically interacts with Put1p in yeast, suggesting that tQM is directly involved in modulating proline levels. Proline 155-162 proline dehydrogenase Saccharomyces cerevisiae S288C 84-89 12796300-8 2003 Our findings show that perceived nitrogen deprivation triggered by rapamycin treatment and steady-state growth in nitrogen-derepressing conditions are associated with hyperphosphorylation of Put3p and increased PUT1 expression. Nitrogen 33-41 proline dehydrogenase Saccharomyces cerevisiae S288C 211-215 12796300-8 2003 Our findings show that perceived nitrogen deprivation triggered by rapamycin treatment and steady-state growth in nitrogen-derepressing conditions are associated with hyperphosphorylation of Put3p and increased PUT1 expression. Nitrogen 114-122 proline dehydrogenase Saccharomyces cerevisiae S288C 211-215 16233323-1 2002 In Saccharomyces cerevisiae, the PUT1-encoded proline oxidase and the PUT2-encoded delta1-pyrroline-5-carboxylate dehydrogenase are required to convert proline to glutamate. Glutamic Acid 163-172 proline dehydrogenase Saccharomyces cerevisiae S288C 33-37 16233323-2 2002 We recently showed that a put1 disruptant accumulated higher levels of proline intracellularly and conferred higher resistance to freezing stress. Proline 71-78 proline dehydrogenase Saccharomyces cerevisiae S288C 26-30 16233323-6 2002 In contrast, the survival rate of the wild-type and the put1-disruptant strains was found to increase after freezing in proportion to their arginine contents. Arginine 140-148 proline dehydrogenase Saccharomyces cerevisiae S288C 56-60 11190469-5 2000 The Pho4p protein that activates transcription of the PH05 gene, a structural gene of acid phosphatase, seems to participate in the negative regulation of the PUT1 and PUT2 genes encoding enzymes of proline catabolism, proline oxidase and delta-pyrroline-5-carboxylate dehydrogenase. Proline 199-206 proline dehydrogenase Saccharomyces cerevisiae S288C 159-163 11401696-1 2001 In Saccharomyces cerevisiae, the ability to use proline as a nitrogen source requires the Put3p transcriptional regulator, which turns on the expression of the proline utilization genes, PUT1 and PUT2, in the presence of the inducer proline and in the absence of preferred nitrogen sources. Proline 48-55 proline dehydrogenase Saccharomyces cerevisiae S288C 187-191 11401696-1 2001 In Saccharomyces cerevisiae, the ability to use proline as a nitrogen source requires the Put3p transcriptional regulator, which turns on the expression of the proline utilization genes, PUT1 and PUT2, in the presence of the inducer proline and in the absence of preferred nitrogen sources. Nitrogen 61-69 proline dehydrogenase Saccharomyces cerevisiae S288C 187-191 11401696-1 2001 In Saccharomyces cerevisiae, the ability to use proline as a nitrogen source requires the Put3p transcriptional regulator, which turns on the expression of the proline utilization genes, PUT1 and PUT2, in the presence of the inducer proline and in the absence of preferred nitrogen sources. Proline 160-167 proline dehydrogenase Saccharomyces cerevisiae S288C 187-191 11401696-1 2001 In Saccharomyces cerevisiae, the ability to use proline as a nitrogen source requires the Put3p transcriptional regulator, which turns on the expression of the proline utilization genes, PUT1 and PUT2, in the presence of the inducer proline and in the absence of preferred nitrogen sources. Proline 160-167 proline dehydrogenase Saccharomyces cerevisiae S288C 187-191 11401696-1 2001 In Saccharomyces cerevisiae, the ability to use proline as a nitrogen source requires the Put3p transcriptional regulator, which turns on the expression of the proline utilization genes, PUT1 and PUT2, in the presence of the inducer proline and in the absence of preferred nitrogen sources. Nitrogen 273-281 proline dehydrogenase Saccharomyces cerevisiae S288C 187-191 9003320-8 1996 The results demonstrated that the removal of free proline during the recovery from salinity or dehydration stress involves an induction of the PDH gene while the activity of P5CS declines. Proline 50-57 proline dehydrogenase Saccharomyces cerevisiae S288C 143-146 9003320-9 1996 The reciprocal regulation of P5CS and PDH genes appears to be a key mechanism in the control of the levels of proline during and after osmotic stress. Proline 110-117 proline dehydrogenase Saccharomyces cerevisiae S288C 38-41 9003320-10 1996 The PDH gene was also significantly induced by exogenously applied proline. Proline 67-74 proline dehydrogenase Saccharomyces cerevisiae S288C 4-7 9003320-11 1996 The induction of PDH by proline, however, was inhibited by salt stress. Proline 24-31 proline dehydrogenase Saccharomyces cerevisiae S288C 17-20 9003320-11 1996 The induction of PDH by proline, however, was inhibited by salt stress. Salts 59-63 proline dehydrogenase Saccharomyces cerevisiae S288C 17-20 8776899-4 1996 Sequence analysis of an Arabidopsis cDNA clone, ERD5 (for early responsive to dehydration stress), isolated from plants dehydrated for 1 hr, revealed that it encodes a protein with identity to products of the yeast PUT1 (for proline utilization) gene (23.6% over 364 amino acids) and the Drosophila sluggish-A gene (34.5% over 255 amino acids). Proline 225-232 proline dehydrogenase Saccharomyces cerevisiae S288C 215-219 8776899-10 1996 Fusion genes for ERD5 and PUT1 complemented a put1 mutant of yeast, allowing put1 to grow with proline as the source of nitrogen. Proline 95-102 proline dehydrogenase Saccharomyces cerevisiae S288C 26-30 8776899-10 1996 Fusion genes for ERD5 and PUT1 complemented a put1 mutant of yeast, allowing put1 to grow with proline as the source of nitrogen. Proline 95-102 proline dehydrogenase Saccharomyces cerevisiae S288C 46-50 8776899-10 1996 Fusion genes for ERD5 and PUT1 complemented a put1 mutant of yeast, allowing put1 to grow with proline as the source of nitrogen. Proline 95-102 proline dehydrogenase Saccharomyces cerevisiae S288C 77-81 8776899-10 1996 Fusion genes for ERD5 and PUT1 complemented a put1 mutant of yeast, allowing put1 to grow with proline as the source of nitrogen. Nitrogen 120-128 proline dehydrogenase Saccharomyces cerevisiae S288C 26-30 8776899-10 1996 Fusion genes for ERD5 and PUT1 complemented a put1 mutant of yeast, allowing put1 to grow with proline as the source of nitrogen. Nitrogen 120-128 proline dehydrogenase Saccharomyces cerevisiae S288C 46-50 8776899-10 1996 Fusion genes for ERD5 and PUT1 complemented a put1 mutant of yeast, allowing put1 to grow with proline as the source of nitrogen. Nitrogen 120-128 proline dehydrogenase Saccharomyces cerevisiae S288C 77-81 8572700-5 1996 Within the proline dehydrogenation domain, several areas of high identity were observed between B. japonicum, E. coli, S. typhimurium, Saccharomyces cerevisiae put1, and Drosophila melanogaster slgA. Proline 11-18 proline dehydrogenase Saccharomyces cerevisiae S288C 160-164 10689174-2 2000 When cultured in liquid minimal medium, the proline-nonutilizing mutant containing the put1 mutation (proline oxidase-deficient) produced more intracellular proline, and increased the cell survival rate as compared to the wild-type strain after freezing and desiccation. Proline 44-51 proline dehydrogenase Saccharomyces cerevisiae S288C 87-91 10689174-2 2000 When cultured in liquid minimal medium, the proline-nonutilizing mutant containing the put1 mutation (proline oxidase-deficient) produced more intracellular proline, and increased the cell survival rate as compared to the wild-type strain after freezing and desiccation. Proline 102-109 proline dehydrogenase Saccharomyces cerevisiae S288C 87-91 10689174-4 2000 PUT1-disrupted mutants in minimal medium supplemented with external proline at 0.1% accumulated higher proline levels than those of the control strains (17-22-fold). Proline 68-75 proline dehydrogenase Saccharomyces cerevisiae S288C 0-4 10689174-4 2000 PUT1-disrupted mutants in minimal medium supplemented with external proline at 0.1% accumulated higher proline levels than those of the control strains (17-22-fold). Proline 103-110 proline dehydrogenase Saccharomyces cerevisiae S288C 0-4 7891726-6 1995 Comparison of PUT gene expression in cells grown in repressing or derepressing nitrogen sources, in the absence of the inducer proline, indicated that both PUT1 and PUT2 are regulated by nitrogen repression, although the effect on PUT2 is comparatively small. Nitrogen 187-195 proline dehydrogenase Saccharomyces cerevisiae S288C 156-160 7891726-7 1995 Recessive mutations in URE2 elevated expression of the PUT1 and PUT2 genes 5- to 10-fold when cells were grown on a nitrogen-repressing medium. Nitrogen 116-124 proline dehydrogenase Saccharomyces cerevisiae S288C 55-59 8416910-1 1993 We demonstrate that expression of the UGA1, CAN1, GAP1, PUT1, PUT2, PUT4, and DAL4 genes is sensitive to nitrogen catabolite repression. Nitrogen 105-113 proline dehydrogenase Saccharomyces cerevisiae S288C 56-60