PMID-sentid Pub_year Sent_text comp_official_name comp_offset protein_name organism prot_offset 14523559-7 2003 SIRT2, SIRT4 and SIRT7 were upregulated, whereas SIRT1, SIRT5 and SIRT6 were downregulated by trichostatin A (TSA) and n-butyrate. trichostatin A 94-108 sirtuin 5 Mus musculus 56-61 14523559-7 2003 SIRT2, SIRT4 and SIRT7 were upregulated, whereas SIRT1, SIRT5 and SIRT6 were downregulated by trichostatin A (TSA) and n-butyrate. trichostatin A 110-113 sirtuin 5 Mus musculus 56-61 14523559-7 2003 SIRT2, SIRT4 and SIRT7 were upregulated, whereas SIRT1, SIRT5 and SIRT6 were downregulated by trichostatin A (TSA) and n-butyrate. Butyrates 119-129 sirtuin 5 Mus musculus 56-61 34245764-4 2021 Furthermore, to define the role of SIRT5 in the carcinogenesis of PDAC, we generated autochthonous mouse models with conditional Sirt5 knockout. pdac 66-70 sirtuin 5 Mus musculus 35-40 34807744-0 2021 Sirtuin 5-Mediated Lysine Desuccinylation Protects Mitochondrial Metabolism Following Subarachnoid Hemorrhage in Mice. Lysine 19-25 sirtuin 5 Mus musculus 0-9 34807744-14 2021 Administration of resveratrol resulted in activation of Sirt5. Resveratrol 18-29 sirtuin 5 Mus musculus 56-61 34807744-16 2021 CONCLUSIONS: Protein lysine succinylation is a biochemical hallmark of metabolic crisis after SAH, and disruption of lysine succinylation through activation of Sirt5 might be a promising therapeutic strategy for the treatment of SAH. Lysine 21-27 sirtuin 5 Mus musculus 160-165 34807744-16 2021 CONCLUSIONS: Protein lysine succinylation is a biochemical hallmark of metabolic crisis after SAH, and disruption of lysine succinylation through activation of Sirt5 might be a promising therapeutic strategy for the treatment of SAH. Lysine 117-123 sirtuin 5 Mus musculus 160-165 34245764-9 2021 Mechanistically, SIRT5 loss enhanced glutamine and glutathione metabolism via acetylation-mediated activation of GOT1. Glutamine 37-46 sirtuin 5 Mus musculus 17-22 34245764-9 2021 Mechanistically, SIRT5 loss enhanced glutamine and glutathione metabolism via acetylation-mediated activation of GOT1. Glutathione 51-62 sirtuin 5 Mus musculus 17-22 34603599-0 2021 SIRT5-Related Desuccinylation Modification Contributes to Quercetin-Induced Protection against Heart Failure and High-Glucose-Prompted Cardiomyocytes Injured through Regulation of Mitochondrial Quality Surveillance. Quercetin 58-67 sirtuin 5 Mus musculus 0-5 34644302-2 2021 TFP is known to be heavily modified by acetyllysine and succinyllysine post-translational modifications (PTMs), many of which are targeted for reversal by the mitochondrial sirtuin deacylases SIRT3 and SIRT5. N-epsilon-Acetyl-L-lysine 39-51 sirtuin 5 Mus musculus 202-207 34644302-2 2021 TFP is known to be heavily modified by acetyllysine and succinyllysine post-translational modifications (PTMs), many of which are targeted for reversal by the mitochondrial sirtuin deacylases SIRT3 and SIRT5. Succinyllysine 56-70 sirtuin 5 Mus musculus 202-207 34644302-4 2021 Here, we mapped the known SIRT3/SIRT5-targeted lysine residues onto the recently solved TFP crystal structure which revealed that many of the target sites are involved in substrate channeling within the TFPalpha subunit. Lysine 47-53 sirtuin 5 Mus musculus 32-37 34642466-0 2022 Repression of p53 function by SIRT5-mediated desuccinylation at Lysine 120 in response to DNA damage. Lysine 64-70 sirtuin 5 Mus musculus 30-35 34603599-11 2021 However, quercetin promoted the desuccinylation of IDH2 by increasing SIRT5 expression. Quercetin 9-18 sirtuin 5 Mus musculus 70-75 34603599-12 2021 Moreover, treatment with si-SIRT5 abolished the protective effect of quercetin on cell viability. Quercetin 69-78 sirtuin 5 Mus musculus 28-33 34603599-13 2021 Hence, quercetin may promote the desuccinylation of IDH2 through SIRT5, maintain mitochondrial homeostasis, protect mouse cardiomyocytes under inflammatory conditions, and improve myocardial fibrosis, thereby reducing the incidence of heart failure. Quercetin 7-16 sirtuin 5 Mus musculus 65-70 34368135-10 2021 The results of mass spectrometry showed increased levels of proteins regulating tricarboxylic acid cycle, oxidative phosphorylation, and fatty acid beta-oxidation pathways in the liver mitochondria of liver SIRT5 OE mice. Tricarboxylic Acids 80-98 sirtuin 5 Mus musculus 207-212 34330933-5 2021 SIRT5 expression was increased in murine PTECs after IRI in vivo and in human PTECs (hPTECs) exposed to an oxygen/nutrient deprivation (OND) model of IRI in vitro. Oxygen 107-113 sirtuin 5 Mus musculus 0-5 34368135-10 2021 The results of mass spectrometry showed increased levels of proteins regulating tricarboxylic acid cycle, oxidative phosphorylation, and fatty acid beta-oxidation pathways in the liver mitochondria of liver SIRT5 OE mice. Fatty Acids 137-147 sirtuin 5 Mus musculus 207-212 34066961-5 2021 We treated 3T3-L1 preadipocytes and mouse primary preadipocyte cultures with the SIRT5 inhibitor MC3482 and investigated the effects of this compound on adipose differentiation and function. MC3482 97-103 sirtuin 5 Mus musculus 81-86 35220131-14 2022 The label-free quantitative proteomic analysis predicted the crucial participation of NAD-dependent protein deacylase sirtuin-5 (SIRT5). NAD 86-89 sirtuin 5 Mus musculus 118-127 35157847-0 2022 Deglutarylation of glutaryl-CoA dehydrogenase by deacylating enzyme SIRT5 promotes lysine oxidation in mice. Lysine 83-89 sirtuin 5 Mus musculus 68-73 35157847-9 2022 Together, these data support a feedback loop model within the lysine/tryptophan oxidation pathway in which glutaryl-CoA is produced, in turn inhibiting GCDH function via glutaryl modification of GCDH lysine residues, and can be relieved by SIRT5 deacylation activity. glutaryl-coenzyme A 107-119 sirtuin 5 Mus musculus 240-245 35613279-6 2022 SIRT5 was engineered to resist acylation-driven inhibition via lysine to arginine mutagenesis. Lysine 63-69 sirtuin 5 Mus musculus 0-5 35613279-6 2022 SIRT5 was engineered to resist acylation-driven inhibition via lysine to arginine mutagenesis. Arginine 73-81 sirtuin 5 Mus musculus 0-5 35220131-14 2022 The label-free quantitative proteomic analysis predicted the crucial participation of NAD-dependent protein deacylase sirtuin-5 (SIRT5). NAD 86-89 sirtuin 5 Mus musculus 129-134 35220131-16 2022 The TSG-supplied inhibition on ROS formation and mitochondrial dysfunction in hepatocytes was disappeared after the application of SIRT5 siRNA. 2,3,5,4'-tetrahydroxystilbene 2-O-glucopyranoside 4-7 sirtuin 5 Mus musculus 131-136 35220131-16 2022 The TSG-supplied inhibition on ROS formation and mitochondrial dysfunction in hepatocytes was disappeared after the application of SIRT5 siRNA. Reactive Oxygen Species 31-34 sirtuin 5 Mus musculus 131-136 35220131-18 2022 Additionally, the TSG-provided inhibition on cellular lipids accumulation was also disappeared in hepatocytes transfected with SIRT5 siRNA. 2,3,5,4'-tetrahydroxystilbene 2-O-glucopyranoside 18-21 sirtuin 5 Mus musculus 127-132 32997407-12 2020 Sirt5-/- ADMSCs exhibited a higher proliferation rate, delayed senescence, and reduced ROS accumulation. ros 87-90 sirtuin 5 Mus musculus 0-5 33836263-7 2021 Moreover, MTX-induced hepatorenal damage were worsened in mice by Sirt5 siRNA or miR-145-5p agomir, which were also alleviated by dioscin. Methotrexate 10-13 sirtuin 5 Mus musculus 66-71 33836263-7 2021 Moreover, MTX-induced hepatorenal damage were worsened in mice by Sirt5 siRNA or miR-145-5p agomir, which were also alleviated by dioscin. dioscin 130-137 sirtuin 5 Mus musculus 66-71 33479498-2 2021 SIRT5 removes negatively charged malonyl, succinyl, and glutaryl groups from lysine residues and thereby regulates multiple enzymes involved in cellular metabolism and other biological processes. Lysine 77-83 sirtuin 5 Mus musculus 0-5 33567897-10 2021 Sirt5 deficiency altered 13 distinct metabolites under basal conditions and 18 metabolites under GluhiInshi treatment. gluhiinshi 97-107 sirtuin 5 Mus musculus 0-5 33567897-11 2021 Pathway analysis identified a wide range of Sirt5-dependent altered metabolic pathways that include amino acid metabolism, TCA cycle, and glycolysis. Trichloroacetic Acid 123-126 sirtuin 5 Mus musculus 44-49 33110171-0 2020 Impaired mitochondrial medium-chain fatty acid oxidation drives periportal macrovesicular steatosis in sirtuin-5 knockout mice. Fatty Acids 36-46 sirtuin 5 Mus musculus 103-112 33110171-3 2020 Coconut oil, but not C8/C10 feeding, induced periportal macrovesicular steatosis in Sirt5KO mice. Coconut Oil 0-11 sirtuin 5 Mus musculus 84-91 33110171-4 2020 14C-C12 degradation was significantly reduced in Sirt5KO liver. 14c-c12 0-7 sirtuin 5 Mus musculus 49-56 33110171-6 2020 Endoplasmic reticulum omega-oxidation, a minor fatty acid degradation pathway known to be stimulated by C12 accumulation, was increased in Sirt5KO liver. Fatty Acids 47-57 sirtuin 5 Mus musculus 139-144 33110171-6 2020 Endoplasmic reticulum omega-oxidation, a minor fatty acid degradation pathway known to be stimulated by C12 accumulation, was increased in Sirt5KO liver. (Z)-14-methylpentadec-2-enoic acid 104-107 sirtuin 5 Mus musculus 139-144 33110171-8 2020 Sirt5KO liver exhibited normal LCAD activity but reduced mitochondrial acyl-CoA synthetase activity with C12. (Z)-14-methylpentadec-2-enoic acid 105-108 sirtuin 5 Mus musculus 0-5 32997407-13 2020 Furthermore, elevated protein succinylation levels were observed in Sirt5-/- ADMSCs, leading to the reduced activity of tricarboxylic acid cycle-related enzymes and attenuated mitochondrial respiration. Tricarboxylic Acids 120-138 sirtuin 5 Mus musculus 68-73 32997407-14 2020 Glucose uptake, glycolysis, and pentose phosphate pathway were elevated in Sirt5-/- ADMSCs. Glucose 0-7 sirtuin 5 Mus musculus 75-80 32997407-14 2020 Glucose uptake, glycolysis, and pentose phosphate pathway were elevated in Sirt5-/- ADMSCs. Pentosephosphates 32-49 sirtuin 5 Mus musculus 75-80 32301534-0 2020 SIRT5 impairs aggregation and activation of the signaling adaptor MAVS through catalyzing lysine desuccinylation. Lysine 90-96 sirtuin 5 Mus musculus 0-5 32301534-5 2020 SIRT5-catalyzed desuccinylation of MAVS at Lysine 7 diminishes the formation of MAVS aggregation after viral infection, resulting in the inhibition of MAVS activation and leading to the impairment of type I IFN production and antiviral gene expression. Lysine 43-49 sirtuin 5 Mus musculus 0-5 31010955-4 2019 Furthermore, we find out that knockdown of SIRT5 reduces intracellular alpha-ketoglutarate concentration, which leads to elevated H3K9me2 and H3K9me3 levels at promoter regions of Ppargamma and Prdm16 loci. Ketoglutaric Acids 71-90 sirtuin 5 Mus musculus 43-48 31575700-0 2019 Sirtuin 5 Regulates Proximal Tubule Fatty Acid Oxidation to Protect against AKI. Fatty Acids 36-46 sirtuin 5 Mus musculus 0-9 31575700-4 2019 Sirtuin 5 (Sirt5), which localizes to both mitochondria and peroxisomes, reverses post-translational lysine acylation on several enzymes involved in fatty acid oxidation. Lysine 101-107 sirtuin 5 Mus musculus 0-9 31575700-4 2019 Sirtuin 5 (Sirt5), which localizes to both mitochondria and peroxisomes, reverses post-translational lysine acylation on several enzymes involved in fatty acid oxidation. Fatty Acids 149-159 sirtuin 5 Mus musculus 0-9 31575700-9 2019 At baseline, Sirt5 knockout (Sirt5-/- ) mice had modestly decreased mitochondrial function but significantly increased fatty acid oxidation, which was localized to the peroxisome. Fatty Acids 119-129 sirtuin 5 Mus musculus 13-18 31575700-9 2019 At baseline, Sirt5 knockout (Sirt5-/- ) mice had modestly decreased mitochondrial function but significantly increased fatty acid oxidation, which was localized to the peroxisome. Fatty Acids 119-129 sirtuin 5 Mus musculus 29-34 31575700-10 2019 Although no overt kidney phenotype was observed in Sirt5-/- mice, Sirt5-/- mice had significantly improved kidney function and less tissue damage compared with controls after either ischemia-induced or cisplatin-induced AKI. Cisplatin 202-211 sirtuin 5 Mus musculus 66-71 31575700-12 2019 CONCLUSIONS: Our findings indicate that Sirt5 regulates the balance of mitochondrial versus peroxisomal fatty acid oxidation in proximal tubular epithelial cells to protect against injury in AKI. Fatty Acids 104-114 sirtuin 5 Mus musculus 40-45 32413611-7 2020 In addition, allicin can significantly increase the succinylation levels of UCP1 in BAT by inhibiting sirt5, whereas excess allicin induces autophagy/mitophagy and mitochondrial dysfunction. allicin 13-20 sirtuin 5 Mus musculus 102-107 30471144-1 2019 Sirt5 is known to functionally regulate mitochondrial proteins by altering posttranslational modifications, including lysine desuccinylation. Lysine 118-124 sirtuin 5 Mus musculus 0-5 30644754-3 2019 To identify regulatory mechanisms attributed to alcohol-induced protein post-translational modifications, we employed a model of alcohol consumption within the context of wild type (WT), sirtuin 3 knockout (SIRT3 KO), and sirtuin 5 knockout (SIRT5 KO) mice to manipulate hepatic mitochondrial protein acylation. Alcohols 48-55 sirtuin 5 Mus musculus 222-231 30644754-3 2019 To identify regulatory mechanisms attributed to alcohol-induced protein post-translational modifications, we employed a model of alcohol consumption within the context of wild type (WT), sirtuin 3 knockout (SIRT3 KO), and sirtuin 5 knockout (SIRT5 KO) mice to manipulate hepatic mitochondrial protein acylation. Alcohols 48-55 sirtuin 5 Mus musculus 242-247 30759120-0 2019 SIRT5 deficiency suppresses mitochondrial ATP production and promotes AMPK activation in response to energy stress. Adenosine Triphosphate 42-45 sirtuin 5 Mus musculus 0-5 30759120-1 2019 Sirtuin 5 (SIRT5) is a member of the NAD+-dependent sirtuin family of protein deacylase that catalyzes removal of post-translational modifications, such as succinylation, malonylation, and glutarylation on lysine residues. NAD 37-40 sirtuin 5 Mus musculus 0-9 30759120-1 2019 Sirtuin 5 (SIRT5) is a member of the NAD+-dependent sirtuin family of protein deacylase that catalyzes removal of post-translational modifications, such as succinylation, malonylation, and glutarylation on lysine residues. NAD 37-40 sirtuin 5 Mus musculus 11-16 30759120-1 2019 Sirtuin 5 (SIRT5) is a member of the NAD+-dependent sirtuin family of protein deacylase that catalyzes removal of post-translational modifications, such as succinylation, malonylation, and glutarylation on lysine residues. Lysine 206-212 sirtuin 5 Mus musculus 0-9 30759120-1 2019 Sirtuin 5 (SIRT5) is a member of the NAD+-dependent sirtuin family of protein deacylase that catalyzes removal of post-translational modifications, such as succinylation, malonylation, and glutarylation on lysine residues. Lysine 206-212 sirtuin 5 Mus musculus 11-16 30759120-2 2019 In light of the SIRT5"s roles in regulating mitochondrion function, we show here that SIRT5 deficiency leads to suppression of mitochondrial NADH oxidation and inhibition of ATP synthase activity. NAD 141-145 sirtuin 5 Mus musculus 16-21 30759120-2 2019 In light of the SIRT5"s roles in regulating mitochondrion function, we show here that SIRT5 deficiency leads to suppression of mitochondrial NADH oxidation and inhibition of ATP synthase activity. NAD 141-145 sirtuin 5 Mus musculus 86-91 30759120-3 2019 As a result, SIRT5 deficiency decreases mitochondrial ATP production, increases AMP/ATP ratio, and subsequently activates AMP-activated protein kinase (AMPK) in cultured cells and mouse hearts under energy stress conditions. Adenosine Triphosphate 54-57 sirtuin 5 Mus musculus 13-18 30759120-3 2019 As a result, SIRT5 deficiency decreases mitochondrial ATP production, increases AMP/ATP ratio, and subsequently activates AMP-activated protein kinase (AMPK) in cultured cells and mouse hearts under energy stress conditions. Adenosine Monophosphate 80-83 sirtuin 5 Mus musculus 13-18 30759120-3 2019 As a result, SIRT5 deficiency decreases mitochondrial ATP production, increases AMP/ATP ratio, and subsequently activates AMP-activated protein kinase (AMPK) in cultured cells and mouse hearts under energy stress conditions. Adenosine Triphosphate 84-87 sirtuin 5 Mus musculus 13-18 30759120-4 2019 Moreover, Sirt5 knockout attenuates transverse aortic constriction (TAC)-induced cardiac hypertrophy and cardiac dysfunction in mice, which is associated with decreased ATP level, increased AMP/ATP ratio and enhanced AMPK activation. Adenosine Triphosphate 169-172 sirtuin 5 Mus musculus 10-15 30759120-4 2019 Moreover, Sirt5 knockout attenuates transverse aortic constriction (TAC)-induced cardiac hypertrophy and cardiac dysfunction in mice, which is associated with decreased ATP level, increased AMP/ATP ratio and enhanced AMPK activation. Adenosine Monophosphate 190-193 sirtuin 5 Mus musculus 10-15 30759120-4 2019 Moreover, Sirt5 knockout attenuates transverse aortic constriction (TAC)-induced cardiac hypertrophy and cardiac dysfunction in mice, which is associated with decreased ATP level, increased AMP/ATP ratio and enhanced AMPK activation. Adenosine Triphosphate 194-197 sirtuin 5 Mus musculus 10-15 29440987-6 2018 Pathway and enrichment analysis of non-targeted primary metabolite profiles from Sirt5-/- cortex revealed alterations in several pathways including purine metabolism (urea, adenosine, adenine, xanthine), nitrogen metabolism (glutamic acid, glycine), and malate-aspartate shuttle (malic acid, glutamic acid). Glutamic Acid 292-305 sirtuin 5 Mus musculus 81-86 30098330-6 2018 SIRT5 overexpression significantly alleviated apoptosis under the High-PA-G condition, accompanied by suppressed Caspase 3 activity and reduced malondialdehyde levels. Protactinium 71-73 sirtuin 5 Mus musculus 0-5 30098330-6 2018 SIRT5 overexpression significantly alleviated apoptosis under the High-PA-G condition, accompanied by suppressed Caspase 3 activity and reduced malondialdehyde levels. Malondialdehyde 144-159 sirtuin 5 Mus musculus 0-5 30098330-7 2018 SIRT5 overexpression also improved beta cell secretory capacity in response to glucose under the High-PA-G condition, suggesting its protective role in beta cell function. Glucose 79-86 sirtuin 5 Mus musculus 0-5 30098330-7 2018 SIRT5 overexpression also improved beta cell secretory capacity in response to glucose under the High-PA-G condition, suggesting its protective role in beta cell function. Protactinium 102-104 sirtuin 5 Mus musculus 0-5 30098330-8 2018 Furthermore, SIRT5 overexpression reversed the decreasing trend of anti-apoptotic factors BCL-2 and BCL-XL expression under High-PA-G condition. Protactinium 129-131 sirtuin 5 Mus musculus 13-18 30017194-3 2018 Sirt5, a sirtuin isoform located in multiple subcellular sites, is involved in regulating a diverse range of cellular and metabolic processes through the removal of a range of acyl-lysine modifications on target proteins. acyl-lysine 176-187 sirtuin 5 Mus musculus 0-5 30017194-4 2018 Loss of Sirt5 leads to hyper-malonylation and hyper-succinylation of both mitochondrial and extra-mitochondrial proteins, influencing oxidative phosphorylation, the TCA cycle and glycolysis. Trichloroacetic Acid 165-168 sirtuin 5 Mus musculus 8-13 29622432-7 2018 Immunohistochemical analysis revealed decreased BBB leakage and degradation of the tight junction protein occludin in SIRT5-/- mice exposed to tMCAO as compared to WT. tmcao 143-148 sirtuin 5 Mus musculus 118-123 29622432-10 2018 CONCLUSION: SIRT5 is upregulated in PBMCs of AIS patients and in the MCA of WT mice exposed to tMCAO; SIRT5 mediates I/R-induced brain damage by increasing BBB permeability through degradation of occludin. tmcao 95-100 sirtuin 5 Mus musculus 102-107 29440987-10 2018 Finally, we found that purine metabolism is a common metabolic pathway regulated by SIRT5, PKCepsilon and ischemic preconditioning. purine 23-29 sirtuin 5 Mus musculus 84-89 30515162-1 2018 Sirtuin 5 (SIRT5) is a member of the family of NAD+-dependent lysine/histone deacetylases. NAD 47-50 sirtuin 5 Mus musculus 0-9 30515162-1 2018 Sirtuin 5 (SIRT5) is a member of the family of NAD+-dependent lysine/histone deacetylases. NAD 47-50 sirtuin 5 Mus musculus 11-16 30515162-2 2018 SIRT5 resides mainly in the mitochondria where it catalyzes deacetylation, demalonylation, desuccinylation, and deglutarylation of lysine to regulate metabolic and oxidative stress response pathways. Lysine 131-137 sirtuin 5 Mus musculus 0-5 30279144-4 2018 METHODS: The hepatic SIRT5-overexpressing ob/ob mouse model (ob/ob-SIRT5 OE) was established by CRISPR/Cas9 gene editing tool Protein malonylation and succinylation lysine sites were identified by immunoprecipitation coupled lipid chromatography - tandem mass spectrometry (LC-MS/MS) methods. Lysine 165-171 sirtuin 5 Mus musculus 21-26 30279144-5 2018 FINDINGS: The ob/ob-SIRT5 OE mice showed decreased malonylation and succinylation, improved cellular glycolysis, suppressed gluconeogenesis, enhanced fatty acid oxidation, and attenuated hepatic steatosis. Fatty Acids 150-160 sirtuin 5 Mus musculus 20-25 29769314-4 2018 To determine whether the survival phenotype we reported was due to a cardiac-intrinsic or cardiac-extrinsic effect of SIRT5, we developed a tamoxifen-inducible, heart-specific SIRT5 knockout (SIRT5KO) mouse model. Tamoxifen 140-149 sirtuin 5 Mus musculus 176-181 29440987-6 2018 Pathway and enrichment analysis of non-targeted primary metabolite profiles from Sirt5-/- cortex revealed alterations in several pathways including purine metabolism (urea, adenosine, adenine, xanthine), nitrogen metabolism (glutamic acid, glycine), and malate-aspartate shuttle (malic acid, glutamic acid). purine 148-154 sirtuin 5 Mus musculus 81-86 29440987-6 2018 Pathway and enrichment analysis of non-targeted primary metabolite profiles from Sirt5-/- cortex revealed alterations in several pathways including purine metabolism (urea, adenosine, adenine, xanthine), nitrogen metabolism (glutamic acid, glycine), and malate-aspartate shuttle (malic acid, glutamic acid). Urea 167-171 sirtuin 5 Mus musculus 81-86 29440987-6 2018 Pathway and enrichment analysis of non-targeted primary metabolite profiles from Sirt5-/- cortex revealed alterations in several pathways including purine metabolism (urea, adenosine, adenine, xanthine), nitrogen metabolism (glutamic acid, glycine), and malate-aspartate shuttle (malic acid, glutamic acid). Adenosine 173-182 sirtuin 5 Mus musculus 81-86 29440987-8 2018 Metabolite changes in SIRT5-/- coincided with alterations in expression of amino acid (SLC7A5, SLC7A7) and glutamate (EAAT2) transport proteins as well as key enzymes in purine (PRPS1, PPAT), fatty acid (ACADS, HADHB), glutamine-glutamate (GAD1, GLUD1), and malate-aspartate shuttle (MDH1) metabolic pathways. Glutamic Acid 107-116 sirtuin 5 Mus musculus 22-27 29440987-8 2018 Metabolite changes in SIRT5-/- coincided with alterations in expression of amino acid (SLC7A5, SLC7A7) and glutamate (EAAT2) transport proteins as well as key enzymes in purine (PRPS1, PPAT), fatty acid (ACADS, HADHB), glutamine-glutamate (GAD1, GLUD1), and malate-aspartate shuttle (MDH1) metabolic pathways. purine 170-176 sirtuin 5 Mus musculus 22-27 29440987-6 2018 Pathway and enrichment analysis of non-targeted primary metabolite profiles from Sirt5-/- cortex revealed alterations in several pathways including purine metabolism (urea, adenosine, adenine, xanthine), nitrogen metabolism (glutamic acid, glycine), and malate-aspartate shuttle (malic acid, glutamic acid). Adenine 184-191 sirtuin 5 Mus musculus 81-86 29440987-8 2018 Metabolite changes in SIRT5-/- coincided with alterations in expression of amino acid (SLC7A5, SLC7A7) and glutamate (EAAT2) transport proteins as well as key enzymes in purine (PRPS1, PPAT), fatty acid (ACADS, HADHB), glutamine-glutamate (GAD1, GLUD1), and malate-aspartate shuttle (MDH1) metabolic pathways. Fatty Acids 192-202 sirtuin 5 Mus musculus 22-27 29440987-8 2018 Metabolite changes in SIRT5-/- coincided with alterations in expression of amino acid (SLC7A5, SLC7A7) and glutamate (EAAT2) transport proteins as well as key enzymes in purine (PRPS1, PPAT), fatty acid (ACADS, HADHB), glutamine-glutamate (GAD1, GLUD1), and malate-aspartate shuttle (MDH1) metabolic pathways. glutamine-glutamate 219-238 sirtuin 5 Mus musculus 22-27 29440987-6 2018 Pathway and enrichment analysis of non-targeted primary metabolite profiles from Sirt5-/- cortex revealed alterations in several pathways including purine metabolism (urea, adenosine, adenine, xanthine), nitrogen metabolism (glutamic acid, glycine), and malate-aspartate shuttle (malic acid, glutamic acid). Xanthine 193-201 sirtuin 5 Mus musculus 81-86 29440987-6 2018 Pathway and enrichment analysis of non-targeted primary metabolite profiles from Sirt5-/- cortex revealed alterations in several pathways including purine metabolism (urea, adenosine, adenine, xanthine), nitrogen metabolism (glutamic acid, glycine), and malate-aspartate shuttle (malic acid, glutamic acid). Nitrogen 204-212 sirtuin 5 Mus musculus 81-86 29440987-6 2018 Pathway and enrichment analysis of non-targeted primary metabolite profiles from Sirt5-/- cortex revealed alterations in several pathways including purine metabolism (urea, adenosine, adenine, xanthine), nitrogen metabolism (glutamic acid, glycine), and malate-aspartate shuttle (malic acid, glutamic acid). Glutamic Acid 225-238 sirtuin 5 Mus musculus 81-86 29440987-6 2018 Pathway and enrichment analysis of non-targeted primary metabolite profiles from Sirt5-/- cortex revealed alterations in several pathways including purine metabolism (urea, adenosine, adenine, xanthine), nitrogen metabolism (glutamic acid, glycine), and malate-aspartate shuttle (malic acid, glutamic acid). Glycine 240-247 sirtuin 5 Mus musculus 81-86 29440987-6 2018 Pathway and enrichment analysis of non-targeted primary metabolite profiles from Sirt5-/- cortex revealed alterations in several pathways including purine metabolism (urea, adenosine, adenine, xanthine), nitrogen metabolism (glutamic acid, glycine), and malate-aspartate shuttle (malic acid, glutamic acid). malate-aspartate 254-270 sirtuin 5 Mus musculus 81-86 29440987-8 2018 Metabolite changes in SIRT5-/- coincided with alterations in expression of amino acid (SLC7A5, SLC7A7) and glutamate (EAAT2) transport proteins as well as key enzymes in purine (PRPS1, PPAT), fatty acid (ACADS, HADHB), glutamine-glutamate (GAD1, GLUD1), and malate-aspartate shuttle (MDH1) metabolic pathways. malic acid 258-264 sirtuin 5 Mus musculus 22-27 29440987-8 2018 Metabolite changes in SIRT5-/- coincided with alterations in expression of amino acid (SLC7A5, SLC7A7) and glutamate (EAAT2) transport proteins as well as key enzymes in purine (PRPS1, PPAT), fatty acid (ACADS, HADHB), glutamine-glutamate (GAD1, GLUD1), and malate-aspartate shuttle (MDH1) metabolic pathways. Aspartic Acid 265-274 sirtuin 5 Mus musculus 22-27 29440987-6 2018 Pathway and enrichment analysis of non-targeted primary metabolite profiles from Sirt5-/- cortex revealed alterations in several pathways including purine metabolism (urea, adenosine, adenine, xanthine), nitrogen metabolism (glutamic acid, glycine), and malate-aspartate shuttle (malic acid, glutamic acid). malic acid 280-290 sirtuin 5 Mus musculus 81-86 27435822-1 2016 Sirtuin 5 (SIRT5) is a mitochondrial-localized NAD(+)-dependent lysine desuccinylase and a major regulator of the mitochondrial succinylome. NAD 47-53 sirtuin 5 Mus musculus 0-9 27681422-7 2016 We further demonstrate that the NAD(+)-dependent mitochondrial deacylases SIRT3 and SIRT5 play important roles in retinal homeostasis and that NAD(+) deficiency causes SIRT3 dysfunction. NAD 32-38 sirtuin 5 Mus musculus 84-89 27435822-1 2016 Sirtuin 5 (SIRT5) is a mitochondrial-localized NAD(+)-dependent lysine desuccinylase and a major regulator of the mitochondrial succinylome. NAD 47-53 sirtuin 5 Mus musculus 11-16 27435822-5 2016 Assessments of mitochondrial bioenergetics in the cortex of wild type (WT) and SIRT5-/- mice revealed that SIRT5 regulates oxygen consumption in the presence of complex I, complex II, and complex IV substrates. Oxygen 123-129 sirtuin 5 Mus musculus 107-112 27435822-7 2016 PKCepsilon-mediated decreases in cell death following oxygen-glucose deprivation were abolished in cortical cultures harvested from SIRT5-/- mice. oxygen-glucose 54-68 sirtuin 5 Mus musculus 132-137 26388266-2 2015 Sirtuin5 (Sirt5) is a mitochondrial NAD(+)-dependent deacylase that catalyzes the removal of succinyl groups from proteins. NAD 36-42 sirtuin 5 Mus musculus 0-8 27051063-2 2016 One such PTM is lysine succinylation, which is regulated by sirtuin 5 (SIRT5). Lysine 16-22 sirtuin 5 Mus musculus 60-69 27051063-2 2016 One such PTM is lysine succinylation, which is regulated by sirtuin 5 (SIRT5). Lysine 16-22 sirtuin 5 Mus musculus 71-76 26001219-7 2016 Use of a pan-sirtuin inhibitor and shRNA-mediated protein knockdown led us to uncover a role for the NAD(+)-dependent family of sirtuins, and in particular for SIRT2 and SIRT5, in the regulation of the necroptotic cell death program. NAD 101-107 sirtuin 5 Mus musculus 170-175 27052737-0 2016 PGC-1alpha Promotes Ureagenesis in Mouse Periportal Hepatocytes through SIRT3 and SIRT5 in Response to Glucagon. Glucagon 103-111 sirtuin 5 Mus musculus 82-87 27052737-5 2016 Glucagon significantly stimulated ureagenesis, expression of SIRT3, SIRT5 and the activities of CPS1 and OCT but did not stimulate PGC-1alpha silencing hepatocytes in mice periportal hepatocytes. Glucagon 0-8 sirtuin 5 Mus musculus 68-73 26388266-14 2015 We further demonstrate that I/R injury in Sirt5(-/-) heart is restored to WT levels by pretreatment with dimethyl malonate, a competitive inhibitor of succinate dehydrogenase (SDH), implicating alteration in SDH activity as causative of the injury. methyl malonate 105-122 sirtuin 5 Mus musculus 42-47 26388266-2 2015 Sirtuin5 (Sirt5) is a mitochondrial NAD(+)-dependent deacylase that catalyzes the removal of succinyl groups from proteins. NAD 36-42 sirtuin 5 Mus musculus 10-15 26388266-8 2015 The 46 unique proteins in Sirt5(-/-) heart participate in metabolic processes such as fatty acid beta-oxidation (Eci2) and branched chain amino acid catabolism, and include respiratory chain proteins (Ndufa7, 12, 13, Dhsa). Fatty Acids 86-96 sirtuin 5 Mus musculus 26-31 26388266-8 2015 The 46 unique proteins in Sirt5(-/-) heart participate in metabolic processes such as fatty acid beta-oxidation (Eci2) and branched chain amino acid catabolism, and include respiratory chain proteins (Ndufa7, 12, 13, Dhsa). chain amino acid 132-148 sirtuin 5 Mus musculus 26-31 25541039-5 2015 SIRT5, which localizes in the mitochondrial matrix, is nicotinamide adenine dinucleotide (NAD(+))-dependent histone deacetylase. NAD 90-96 sirtuin 5 Mus musculus 0-5 25541039-0 2015 Protective role of SIRT5 against motor deficit and dopaminergic degeneration in MPTP-induced mice model of Parkinson"s disease. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine 80-84 sirtuin 5 Mus musculus 19-24 26073543-3 2015 Using affinity enrichment and label free quantitative proteomics, we characterized the SIRT5-regulated lysine malonylome in wild-type (WT) and Sirt5(-/-) mice. Lysine 103-109 sirtuin 5 Mus musculus 87-92 26073543-9 2015 These data demonstrate that SIRT5 is a global regulator of lysine malonylation and provide a mechanism for regulation of energetic flux through glycolysis. Lysine 59-65 sirtuin 5 Mus musculus 28-33 25541039-7 2015 Here, we investigated the role of SIRT5 in the pathogenesis of PD mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine 82-126 sirtuin 5 Mus musculus 34-39 25541039-5 2015 SIRT5, which localizes in the mitochondrial matrix, is nicotinamide adenine dinucleotide (NAD(+))-dependent histone deacetylase. NAD 55-88 sirtuin 5 Mus musculus 0-5 25541039-7 2015 Here, we investigated the role of SIRT5 in the pathogenesis of PD mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine 128-132 sirtuin 5 Mus musculus 34-39 25541039-8 2015 We present evidence that SIRT5 deficiency, by itself, does not affect motor and non-motor functions; however, lack of SIRT5 exacerbates MPTP-induced motor deficits. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine 136-140 sirtuin 5 Mus musculus 118-123 25541039-9 2015 Consistently, MPTP-exposed SIRT5 knockout mice exhibited more severe nigrostriatal dopaminergic degeneration than that observed in wild-type controls. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine 14-18 sirtuin 5 Mus musculus 27-32 25541039-10 2015 Furthermore, deletion of SIRT5 leads to a larger decrease, relative to control, in the expression level of manganese superoxide dismutase (SOD2), a mitochondria-specific antioxidant enzyme, after MPTP induction. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine 196-200 sirtuin 5 Mus musculus 25-30 25541039-11 2015 These findings indicate that SIRT5 ameliorates MPTP-induced nigrostriatal dopaminergic degeneration via preserving mitochondrial antioxidant capacity. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine 47-51 sirtuin 5 Mus musculus 29-34 24687991-8 2014 The antidiabetes drug metformin (1 mM), an established AMPK activator, reduced the mouse SIRT5 protein level by 44% in cultured hepatocytes and by 31% in liver in vivo (300 mg/kg, 7 d). Metformin 22-31 sirtuin 5 Mus musculus 89-94 24703693-0 2014 Lysine glutarylation is a protein posttranslational modification regulated by SIRT5. Lysine 0-6 sirtuin 5 Mus musculus 78-83 24703693-5 2014 We validated carbamoyl phosphate synthase 1 (CPS1), the rate-limiting enzyme in urea cycle, as a glutarylated protein and demonstrated that CPS1 is targeted by SIRT5 for deglutarylation. Urea 80-84 sirtuin 5 Mus musculus 160-165 23673559-0 2013 Cloning, chromosomal characterization and FISH mapping of the NAD(+)-dependent histone deacetylase gene sirtuin 5 in the mouse. NAD 62-68 sirtuin 5 Mus musculus 104-113 23673559-1 2013 Sirtuin 5 (SIRT5) is a nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase, belonging to the silent information regulator 2 (Sir2) family of sirtuin histone deacetylases (sirtuins). NAD 58-61 sirtuin 5 Mus musculus 11-16 23673559-1 2013 Sirtuin 5 (SIRT5) is a nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase, belonging to the silent information regulator 2 (Sir2) family of sirtuin histone deacetylases (sirtuins). NAD 23-56 sirtuin 5 Mus musculus 0-9 23673559-1 2013 Sirtuin 5 (SIRT5) is a nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase, belonging to the silent information regulator 2 (Sir2) family of sirtuin histone deacetylases (sirtuins). NAD 23-56 sirtuin 5 Mus musculus 11-16 23673559-1 2013 Sirtuin 5 (SIRT5) is a nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase, belonging to the silent information regulator 2 (Sir2) family of sirtuin histone deacetylases (sirtuins). NAD 58-61 sirtuin 5 Mus musculus 0-9 20097174-4 2010 We identified carbamoyl phosphate synthetase 1 (CPS1), a key enzyme of the urea cycle that catalyzes condensation of ammonia with bicarbonate to form carbamoyl phosphate, as a target of SIRT5 by two-dimensional electrophoresis comparing mitochondrial proteins in livers of SIRT5 Tg and wild-type mice. Urea 75-79 sirtuin 5 Mus musculus 186-191 22992439-8 2012 Results indicated that the expression of sirt1, sirt5 and sirt6 was significant downregulated by nicotinamide treatment. Niacinamide 97-109 sirtuin 5 Mus musculus 48-53 20097174-4 2010 We identified carbamoyl phosphate synthetase 1 (CPS1), a key enzyme of the urea cycle that catalyzes condensation of ammonia with bicarbonate to form carbamoyl phosphate, as a target of SIRT5 by two-dimensional electrophoresis comparing mitochondrial proteins in livers of SIRT5 Tg and wild-type mice. Urea 75-79 sirtuin 5 Mus musculus 273-278 19410549-0 2009 SIRT5 Deacetylates carbamoyl phosphate synthetase 1 and regulates the urea cycle. Urea 70-74 sirtuin 5 Mus musculus 0-5 19410549-3 2009 Here, we show that SIRT5 localizes in the mitochondrial matrix and interacts with carbamoyl phosphate synthetase 1 (CPS1), an enzyme, catalyzing the initial step of the urea cycle for ammonia detoxification and disposal. Urea 169-173 sirtuin 5 Mus musculus 19-24 19410549-3 2009 Here, we show that SIRT5 localizes in the mitochondrial matrix and interacts with carbamoyl phosphate synthetase 1 (CPS1), an enzyme, catalyzing the initial step of the urea cycle for ammonia detoxification and disposal. Ammonia 184-191 sirtuin 5 Mus musculus 19-24 19410549-5 2009 During fasting, NAD in liver mitochondria increases, thereby triggering SIRT5 deacetylation of CPS1 and adaptation to the increase in amino acid catabolism. NAD 16-19 sirtuin 5 Mus musculus 72-77 19410549-6 2009 Indeed, SIRT5 KO mice fail to upregulate CPS1 activity and show elevated blood ammonia during fasting. Ammonia 79-86 sirtuin 5 Mus musculus 8-13 19410549-8 2009 These findings demonstrate SIRT5 plays a pivotal role in ammonia detoxification and disposal by activating CPS1. Ammonia 57-64 sirtuin 5 Mus musculus 27-32 20097174-4 2010 We identified carbamoyl phosphate synthetase 1 (CPS1), a key enzyme of the urea cycle that catalyzes condensation of ammonia with bicarbonate to form carbamoyl phosphate, as a target of SIRT5 by two-dimensional electrophoresis comparing mitochondrial proteins in livers of SIRT5 Tg and wild-type mice. Ammonia 117-124 sirtuin 5 Mus musculus 186-191 20097174-4 2010 We identified carbamoyl phosphate synthetase 1 (CPS1), a key enzyme of the urea cycle that catalyzes condensation of ammonia with bicarbonate to form carbamoyl phosphate, as a target of SIRT5 by two-dimensional electrophoresis comparing mitochondrial proteins in livers of SIRT5 Tg and wild-type mice. Ammonia 117-124 sirtuin 5 Mus musculus 273-278 20097174-4 2010 We identified carbamoyl phosphate synthetase 1 (CPS1), a key enzyme of the urea cycle that catalyzes condensation of ammonia with bicarbonate to form carbamoyl phosphate, as a target of SIRT5 by two-dimensional electrophoresis comparing mitochondrial proteins in livers of SIRT5 Tg and wild-type mice. Bicarbonates 130-141 sirtuin 5 Mus musculus 186-191 20097174-4 2010 We identified carbamoyl phosphate synthetase 1 (CPS1), a key enzyme of the urea cycle that catalyzes condensation of ammonia with bicarbonate to form carbamoyl phosphate, as a target of SIRT5 by two-dimensional electrophoresis comparing mitochondrial proteins in livers of SIRT5 Tg and wild-type mice. Bicarbonates 130-141 sirtuin 5 Mus musculus 273-278 20097174-4 2010 We identified carbamoyl phosphate synthetase 1 (CPS1), a key enzyme of the urea cycle that catalyzes condensation of ammonia with bicarbonate to form carbamoyl phosphate, as a target of SIRT5 by two-dimensional electrophoresis comparing mitochondrial proteins in livers of SIRT5 Tg and wild-type mice. Carbamyl Phosphate 14-33 sirtuin 5 Mus musculus 186-191 20097174-4 2010 We identified carbamoyl phosphate synthetase 1 (CPS1), a key enzyme of the urea cycle that catalyzes condensation of ammonia with bicarbonate to form carbamoyl phosphate, as a target of SIRT5 by two-dimensional electrophoresis comparing mitochondrial proteins in livers of SIRT5 Tg and wild-type mice. Carbamyl Phosphate 14-33 sirtuin 5 Mus musculus 273-278 20097174-6 2010 In addition, urea production was upregulated in hepatocytes of SIRT5 Tg mice. Urea 13-17 sirtuin 5 Mus musculus 63-68 20097174-8 2010 Because ammonia generated during fasting is toxic, SIRT5 protein might play a protective role by converting ammonia to non-toxic urea through deacetylation and activation of CPS1. Ammonia 8-15 sirtuin 5 Mus musculus 51-56 20097174-8 2010 Because ammonia generated during fasting is toxic, SIRT5 protein might play a protective role by converting ammonia to non-toxic urea through deacetylation and activation of CPS1. Ammonia 108-115 sirtuin 5 Mus musculus 51-56 20097174-8 2010 Because ammonia generated during fasting is toxic, SIRT5 protein might play a protective role by converting ammonia to non-toxic urea through deacetylation and activation of CPS1. Urea 129-133 sirtuin 5 Mus musculus 51-56