PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 9186492-4 1997 Mixing of preformed beta (1-40) amyloid fibrils with ThT in a stopped-flow spectrophotometer, monitoring fluorescence emission at > 475 nm while exciting at 450 nm, distinguished multiple kinetic phases of roughly equivalent amplitude with tau"s in the ranges of 0.007, 0.05, 0.75, and 10-20 s. The fastest reaction appears to reflect a bimolecular dye binding event while the remaining reactions are rate-limited by protein tertiary or quaternary conformational changes. thioflavin T 53-56 microtubule associated protein tau Homo sapiens 240-243