PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 3085533-2 1986 The method is based on (1) the high-affinity binding (Kp = 1.4 +/- 2 nM) of tPA to a solid-phase fibrin network constructed by thrombin proteolysis of fibrinogen covalently coupled to polyglutaraldehyde-activated polyvinyl chloride microtiter plates, and (2) the subsequent development of PA activity by the fibrin-tPA complex and its measurement with a coupled assay using a chromogenic substrate highly selective for plasmin. Protactinium 77-79 coagulation factor II, thrombin Homo sapiens 127-135