PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 26464590-7 2015 Expression level analysis indicated that PTEN reached its peak level at 36( )C for 8 h with 1.5625mM IPTG, while solubility analysis revealed the optimal induction temperature was at 20( )C, the optimal IPTG concentration was 0.1microM and the optimal induction time was up to 8 h. Taken together, we provide an optimal induction condition for expressing soluble fusion protein of PTEN in E. coli, facilitating further analysis of PTEN"s biological function in vitro. Isopropyl Thiogalactoside 101-105 phosphatase and tensin homolog Homo sapiens 41-45 26464590-7 2015 Expression level analysis indicated that PTEN reached its peak level at 36( )C for 8 h with 1.5625mM IPTG, while solubility analysis revealed the optimal induction temperature was at 20( )C, the optimal IPTG concentration was 0.1microM and the optimal induction time was up to 8 h. Taken together, we provide an optimal induction condition for expressing soluble fusion protein of PTEN in E. coli, facilitating further analysis of PTEN"s biological function in vitro. Isopropyl Thiogalactoside 203-207 phosphatase and tensin homolog Homo sapiens 41-45 26464590-7 2015 Expression level analysis indicated that PTEN reached its peak level at 36( )C for 8 h with 1.5625mM IPTG, while solubility analysis revealed the optimal induction temperature was at 20( )C, the optimal IPTG concentration was 0.1microM and the optimal induction time was up to 8 h. Taken together, we provide an optimal induction condition for expressing soluble fusion protein of PTEN in E. coli, facilitating further analysis of PTEN"s biological function in vitro. Isopropyl Thiogalactoside 203-207 phosphatase and tensin homolog Homo sapiens 381-385 26464590-7 2015 Expression level analysis indicated that PTEN reached its peak level at 36( )C for 8 h with 1.5625mM IPTG, while solubility analysis revealed the optimal induction temperature was at 20( )C, the optimal IPTG concentration was 0.1microM and the optimal induction time was up to 8 h. Taken together, we provide an optimal induction condition for expressing soluble fusion protein of PTEN in E. coli, facilitating further analysis of PTEN"s biological function in vitro. Isopropyl Thiogalactoside 203-207 phosphatase and tensin homolog Homo sapiens 381-385