PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 20044597-0 2009 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is pyruvylated during 3-bromopyruvate mediated cancer cell death. bromopyruvate 71-86 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 0-40 20044597-0 2009 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is pyruvylated during 3-bromopyruvate mediated cancer cell death. bromopyruvate 71-86 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 42-47 20044597-4 2009 METHODS AND RESULTS: In the current study, using radiolabeled ((14)C) 3BrPA in multiple cancer cell lines, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified as the primary intracellular target of 3BrPA, based on two-dimensional (2D) gel electrophoretic autoradiography, mass spectrometry and immunoprecipitation. bromopyruvate 70-75 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 107-147 20044597-4 2009 METHODS AND RESULTS: In the current study, using radiolabeled ((14)C) 3BrPA in multiple cancer cell lines, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified as the primary intracellular target of 3BrPA, based on two-dimensional (2D) gel electrophoretic autoradiography, mass spectrometry and immunoprecipitation. bromopyruvate 70-75 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 149-154 20044597-4 2009 METHODS AND RESULTS: In the current study, using radiolabeled ((14)C) 3BrPA in multiple cancer cell lines, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified as the primary intracellular target of 3BrPA, based on two-dimensional (2D) gel electrophoretic autoradiography, mass spectrometry and immunoprecipitation. bromopyruvate 210-215 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 107-147 20044597-4 2009 METHODS AND RESULTS: In the current study, using radiolabeled ((14)C) 3BrPA in multiple cancer cell lines, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified as the primary intracellular target of 3BrPA, based on two-dimensional (2D) gel electrophoretic autoradiography, mass spectrometry and immunoprecipitation. bromopyruvate 210-215 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 149-154 20044597-5 2009 Furthermore, in vitro enzyme kinetic studies established that 3BrPA has marked affinity to GAPDH. bromopyruvate 62-67 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 91-96 20044597-7 2009 CONCLUSION: GAPDH pyruvylation by 3BrPA affects its enzymatic function and is the primary intracellular target in 3BrPA mediated cancer cell death. bromopyruvate 34-39 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 12-17 20044597-7 2009 CONCLUSION: GAPDH pyruvylation by 3BrPA affects its enzymatic function and is the primary intracellular target in 3BrPA mediated cancer cell death. bromopyruvate 114-119 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 12-17