PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 16656864-1 1968 With respect to cofactor requirements, NADH, and FMNH(2) were equally effective as electron donors for nitrate reductase obtained from leaves of maize, marrow, and spinach, when the cofactors were supplied in optimal concentrations. NAD 39-43 nitrate reductase [NADH] 1 Zea mays 103-120 16656864-5 1968 Unity of NADH to FMNH(2) activities were obtained during: A) purification procedures (4 step, 30-fold); B) induction of nitrate reductase in corn seedlings with nitrate; and C) inactivation of nitrate reductase in intact or excised corn seedlings. NAD 9-13 nitrate reductase [NADH] 1 Zea mays 120-137 16656864-5 1968 Unity of NADH to FMNH(2) activities were obtained during: A) purification procedures (4 step, 30-fold); B) induction of nitrate reductase in corn seedlings with nitrate; and C) inactivation of nitrate reductase in intact or excised corn seedlings. NAD 9-13 nitrate reductase [NADH] 1 Zea mays 193-210 16656864-6 1968 The NADH- and FMNH(2)-dependent activities were not additive.A half-life for nitrate reductase of approximately 4 hours was estimated from the inactivation studies with excised corn seedlings. NAD 4-8 nitrate reductase [NADH] 1 Zea mays 77-94 16656864-8 1968 This was verified by reactivation with exogenous cysteine.Based on these current findings, and previous work, it is concluded that nitrate reductase is a single moiety with the ability to utilize either NADH or FMNH(2) as cofactor. NAD 203-207 nitrate reductase [NADH] 1 Zea mays 131-148 16656864-9 1968 However the high concentration of FMNH(2) required for optimal activity suggests that in vivo NADH is the electron donor and that nitrate reductase in higher plants should be designated NADH:nitrate reductase (E.C. NAD 94-98 nitrate reductase [NADH] 1 Zea mays 191-208 16656864-9 1968 However the high concentration of FMNH(2) required for optimal activity suggests that in vivo NADH is the electron donor and that nitrate reductase in higher plants should be designated NADH:nitrate reductase (E.C. NAD 186-190 nitrate reductase [NADH] 1 Zea mays 130-147 16656864-9 1968 However the high concentration of FMNH(2) required for optimal activity suggests that in vivo NADH is the electron donor and that nitrate reductase in higher plants should be designated NADH:nitrate reductase (E.C. NAD 186-190 nitrate reductase [NADH] 1 Zea mays 191-208