PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 15197535-2 2004 Insulin secretion was determined by a radioimmunoassay (RIA), cytosolic free Ca2+ concentration ([Ca2+]c) with the fluorescence dye fura-2 and whole-cell membrane currents with the patch-clamp technique. Fura-2 132-138 insulin Homo sapiens 0-7