PMID-sentid Pub_year Sent_text compound_name comp_offset prot_official_name organism prot_offset 33373321-4 2020 We found that miR-223 was downregulated in PDGF-BB-treated HASMCs in a dose- and time-dependent manner, while nuclear factor of activated T cells 5 (NFAT5) was upregulated. hasmcs 59-65 microRNA 223 Homo sapiens 14-21 31468685-2 2019 This study determined the expression of lncRNA TNK2 antisense RNA 1 (TNK2-AS1) in oxidized low-density lipoprotein (ox-LDL)-stimulated human aortic smooth muscle cells (HASMCs) and examined the mechanistic role of TNK2-AS1 in the proliferation and migration of HASMCs. hasmcs 169-175 TNK2 antisense RNA 1 Homo sapiens 47-67 33437796-12 2020 In vitro experiments indicated that ICOS overexpression reduces phagocytosis and proliferation by HASMCs, and may therefore produce an anti-atherosclerotic effect. hasmcs 98-104 inducible T-cell co-stimulator Rattus norvegicus 36-40 31448474-8 2019 Furthermore, LY294002, a specific inhibitor of PI3K/Akt pathway, attenuated the activation of NK-kappaB and osteogenic differentiation of HASMCs. hasmcs 138-144 AKT serine/threonine kinase 1 Homo sapiens 52-55 31468685-2 2019 This study determined the expression of lncRNA TNK2 antisense RNA 1 (TNK2-AS1) in oxidized low-density lipoprotein (ox-LDL)-stimulated human aortic smooth muscle cells (HASMCs) and examined the mechanistic role of TNK2-AS1 in the proliferation and migration of HASMCs. hasmcs 169-175 TNK2 antisense RNA 1 Homo sapiens 69-77 31468685-3 2019 Our results demonstrated that ox-LDL promoted HASMC proliferation and migration, and the enhanced proliferation and migration in ox-LDL-treated HASMCs were accompanied by the up-regulation of TNK2-AS1. hasmcs 144-150 TNK2 antisense RNA 1 Homo sapiens 192-200 31468685-4 2019 In vitro functional studies showed that TNK2-AS1 knockdown suppressed cell proliferation and migration of ox-LDL-stimulated HASMCs, while TNK2-AS1 overexpression enhanced HASMC proliferation and migration. hasmcs 124-130 TNK2 antisense RNA 1 Homo sapiens 40-48 31468685-4 2019 In vitro functional studies showed that TNK2-AS1 knockdown suppressed cell proliferation and migration of ox-LDL-stimulated HASMCs, while TNK2-AS1 overexpression enhanced HASMC proliferation and migration. hasmcs 124-130 tyrosine kinase non receptor 2 Homo sapiens 40-44 31468685-4 2019 In vitro functional studies showed that TNK2-AS1 knockdown suppressed cell proliferation and migration of ox-LDL-stimulated HASMCs, while TNK2-AS1 overexpression enhanced HASMC proliferation and migration. hasmcs 124-130 prostaglandin D2 receptor Homo sapiens 45-48 31468685-6 2019 Moreover, miR-150-5p could target the 3" untranslated regions of vascular endothelial growth factor A (VEGFA) and fibroblast growth factor 1 (FGF1) to regulate FGF1 and VEGFA expression in HASMCs, and the inhibitory effects of miR-150-5p overexpression in ox-LDL-stimulated HASMCs were attenuated by enforced expression of VEGFA and FGF1. hasmcs 189-195 vascular endothelial growth factor A Homo sapiens 65-101 31468685-6 2019 Moreover, miR-150-5p could target the 3" untranslated regions of vascular endothelial growth factor A (VEGFA) and fibroblast growth factor 1 (FGF1) to regulate FGF1 and VEGFA expression in HASMCs, and the inhibitory effects of miR-150-5p overexpression in ox-LDL-stimulated HASMCs were attenuated by enforced expression of VEGFA and FGF1. hasmcs 189-195 vascular endothelial growth factor A Homo sapiens 103-108 31468685-6 2019 Moreover, miR-150-5p could target the 3" untranslated regions of vascular endothelial growth factor A (VEGFA) and fibroblast growth factor 1 (FGF1) to regulate FGF1 and VEGFA expression in HASMCs, and the inhibitory effects of miR-150-5p overexpression in ox-LDL-stimulated HASMCs were attenuated by enforced expression of VEGFA and FGF1. hasmcs 189-195 fibroblast growth factor 1 Homo sapiens 114-140 31468685-6 2019 Moreover, miR-150-5p could target the 3" untranslated regions of vascular endothelial growth factor A (VEGFA) and fibroblast growth factor 1 (FGF1) to regulate FGF1 and VEGFA expression in HASMCs, and the inhibitory effects of miR-150-5p overexpression in ox-LDL-stimulated HASMCs were attenuated by enforced expression of VEGFA and FGF1. hasmcs 189-195 fibroblast growth factor 1 Homo sapiens 142-146 31468685-6 2019 Moreover, miR-150-5p could target the 3" untranslated regions of vascular endothelial growth factor A (VEGFA) and fibroblast growth factor 1 (FGF1) to regulate FGF1 and VEGFA expression in HASMCs, and the inhibitory effects of miR-150-5p overexpression in ox-LDL-stimulated HASMCs were attenuated by enforced expression of VEGFA and FGF1. hasmcs 189-195 fibroblast growth factor 1 Homo sapiens 160-164 31468685-6 2019 Moreover, miR-150-5p could target the 3" untranslated regions of vascular endothelial growth factor A (VEGFA) and fibroblast growth factor 1 (FGF1) to regulate FGF1 and VEGFA expression in HASMCs, and the inhibitory effects of miR-150-5p overexpression in ox-LDL-stimulated HASMCs were attenuated by enforced expression of VEGFA and FGF1. hasmcs 189-195 vascular endothelial growth factor A Homo sapiens 169-174 31468685-6 2019 Moreover, miR-150-5p could target the 3" untranslated regions of vascular endothelial growth factor A (VEGFA) and fibroblast growth factor 1 (FGF1) to regulate FGF1 and VEGFA expression in HASMCs, and the inhibitory effects of miR-150-5p overexpression in ox-LDL-stimulated HASMCs were attenuated by enforced expression of VEGFA and FGF1. hasmcs 189-195 vascular endothelial growth factor A Homo sapiens 169-174 31468685-6 2019 Moreover, miR-150-5p could target the 3" untranslated regions of vascular endothelial growth factor A (VEGFA) and fibroblast growth factor 1 (FGF1) to regulate FGF1 and VEGFA expression in HASMCs, and the inhibitory effects of miR-150-5p overexpression in ox-LDL-stimulated HASMCs were attenuated by enforced expression of VEGFA and FGF1. hasmcs 189-195 fibroblast growth factor 1 Homo sapiens 160-164 31468685-6 2019 Moreover, miR-150-5p could target the 3" untranslated regions of vascular endothelial growth factor A (VEGFA) and fibroblast growth factor 1 (FGF1) to regulate FGF1 and VEGFA expression in HASMCs, and the inhibitory effects of miR-150-5p overexpression in ox-LDL-stimulated HASMCs were attenuated by enforced expression of VEGFA and FGF1. hasmcs 274-280 vascular endothelial growth factor A Homo sapiens 65-101 31468685-6 2019 Moreover, miR-150-5p could target the 3" untranslated regions of vascular endothelial growth factor A (VEGFA) and fibroblast growth factor 1 (FGF1) to regulate FGF1 and VEGFA expression in HASMCs, and the inhibitory effects of miR-150-5p overexpression in ox-LDL-stimulated HASMCs were attenuated by enforced expression of VEGFA and FGF1. hasmcs 274-280 vascular endothelial growth factor A Homo sapiens 103-108 31468685-6 2019 Moreover, miR-150-5p could target the 3" untranslated regions of vascular endothelial growth factor A (VEGFA) and fibroblast growth factor 1 (FGF1) to regulate FGF1 and VEGFA expression in HASMCs, and the inhibitory effects of miR-150-5p overexpression in ox-LDL-stimulated HASMCs were attenuated by enforced expression of VEGFA and FGF1. hasmcs 274-280 fibroblast growth factor 1 Homo sapiens 114-140 31468685-6 2019 Moreover, miR-150-5p could target the 3" untranslated regions of vascular endothelial growth factor A (VEGFA) and fibroblast growth factor 1 (FGF1) to regulate FGF1 and VEGFA expression in HASMCs, and the inhibitory effects of miR-150-5p overexpression in ox-LDL-stimulated HASMCs were attenuated by enforced expression of VEGFA and FGF1. hasmcs 274-280 fibroblast growth factor 1 Homo sapiens 142-146 31468685-6 2019 Moreover, miR-150-5p could target the 3" untranslated regions of vascular endothelial growth factor A (VEGFA) and fibroblast growth factor 1 (FGF1) to regulate FGF1 and VEGFA expression in HASMCs, and the inhibitory effects of miR-150-5p overexpression in ox-LDL-stimulated HASMCs were attenuated by enforced expression of VEGFA and FGF1. hasmcs 274-280 fibroblast growth factor 1 Homo sapiens 160-164 31468685-6 2019 Moreover, miR-150-5p could target the 3" untranslated regions of vascular endothelial growth factor A (VEGFA) and fibroblast growth factor 1 (FGF1) to regulate FGF1 and VEGFA expression in HASMCs, and the inhibitory effects of miR-150-5p overexpression in ox-LDL-stimulated HASMCs were attenuated by enforced expression of VEGFA and FGF1. hasmcs 274-280 vascular endothelial growth factor A Homo sapiens 169-174 31468685-6 2019 Moreover, miR-150-5p could target the 3" untranslated regions of vascular endothelial growth factor A (VEGFA) and fibroblast growth factor 1 (FGF1) to regulate FGF1 and VEGFA expression in HASMCs, and the inhibitory effects of miR-150-5p overexpression in ox-LDL-stimulated HASMCs were attenuated by enforced expression of VEGFA and FGF1. hasmcs 274-280 vascular endothelial growth factor A Homo sapiens 169-174 31468685-6 2019 Moreover, miR-150-5p could target the 3" untranslated regions of vascular endothelial growth factor A (VEGFA) and fibroblast growth factor 1 (FGF1) to regulate FGF1 and VEGFA expression in HASMCs, and the inhibitory effects of miR-150-5p overexpression in ox-LDL-stimulated HASMCs were attenuated by enforced expression of VEGFA and FGF1. hasmcs 274-280 fibroblast growth factor 1 Homo sapiens 160-164 31468685-7 2019 Enforced expression of VEGFA and FGF1 also partially restored the suppressed cell proliferation and migration induced by TNK2-AS1 knockdown in ox-LDL-stimulated HASMCs, while the enhanced effects of TNK2-AS1 overexpression on HASMC proliferation and migration were attenuated by the knockdown of VEGFA and FGF1. hasmcs 161-167 vascular endothelial growth factor A Homo sapiens 23-28 31468685-7 2019 Enforced expression of VEGFA and FGF1 also partially restored the suppressed cell proliferation and migration induced by TNK2-AS1 knockdown in ox-LDL-stimulated HASMCs, while the enhanced effects of TNK2-AS1 overexpression on HASMC proliferation and migration were attenuated by the knockdown of VEGFA and FGF1. hasmcs 161-167 fibroblast growth factor 1 Homo sapiens 33-37 31468685-7 2019 Enforced expression of VEGFA and FGF1 also partially restored the suppressed cell proliferation and migration induced by TNK2-AS1 knockdown in ox-LDL-stimulated HASMCs, while the enhanced effects of TNK2-AS1 overexpression on HASMC proliferation and migration were attenuated by the knockdown of VEGFA and FGF1. hasmcs 161-167 TNK2 antisense RNA 1 Homo sapiens 121-129 31468685-8 2019 Collectively, our findings showed that TNK2-AS1 exerted its action in ox-LDL-stimulated HASMCs via regulating VEGFA and FGF1 expression by acting as a ceRNA for miR-150-5p. hasmcs 88-94 TNK2 antisense RNA 1 Homo sapiens 39-47 31468685-8 2019 Collectively, our findings showed that TNK2-AS1 exerted its action in ox-LDL-stimulated HASMCs via regulating VEGFA and FGF1 expression by acting as a ceRNA for miR-150-5p. hasmcs 88-94 vascular endothelial growth factor A Homo sapiens 110-115 31468685-8 2019 Collectively, our findings showed that TNK2-AS1 exerted its action in ox-LDL-stimulated HASMCs via regulating VEGFA and FGF1 expression by acting as a ceRNA for miR-150-5p. hasmcs 88-94 fibroblast growth factor 1 Homo sapiens 120-124 31351049-5 2019 The results showed that TRIM28 was highly expressed in human atherosclerotic tissues, as well in cultured HASMCs stimulated by platelet-derived growth factor subunit B homodimer (PDGF-BB). hasmcs 106-112 tripartite motif containing 28 Homo sapiens 24-30 31351049-6 2019 Knockdown of TRIM28 by transfection with siRNA targeting TRIM28 (si-TRIM28) significantly suppressed the PDGF-BB-induced cell proliferation and migration of HASMCs. hasmcs 157-163 tripartite motif containing 28 Homo sapiens 13-19 31351049-6 2019 Knockdown of TRIM28 by transfection with siRNA targeting TRIM28 (si-TRIM28) significantly suppressed the PDGF-BB-induced cell proliferation and migration of HASMCs. hasmcs 157-163 tripartite motif containing 28 Homo sapiens 57-63 31351049-6 2019 Knockdown of TRIM28 by transfection with siRNA targeting TRIM28 (si-TRIM28) significantly suppressed the PDGF-BB-induced cell proliferation and migration of HASMCs. hasmcs 157-163 tripartite motif containing 28 Homo sapiens 65-74 31351049-9 2019 Furthermore, knockdown of TRIM28 blocked PDGF-BB-induced NF-kappaB activation in HASMCs. hasmcs 81-87 tripartite motif containing 28 Homo sapiens 26-32 31351049-10 2019 Collectively, knockdown of TRIM28 prevented PDGF-BB-induced phenotypic switching of HASMCs, which might be mediated by the regulation of NF-kappaB signaling pathway. hasmcs 84-90 tripartite motif containing 28 Homo sapiens 27-33 31253783-8 2019 Pioglitazone significantly rescues excessive intimal hyperplasia in Nlrc5-/- mice and attenuates the increased proliferation and dedifferentiation in NLRC5-deficient HASMCs. hasmcs 166-172 NLR family, CARD domain containing 5 Mus musculus 150-155 31235554-7 2019 PTENP1 silencing exhibited the opposite effects and mitigated H2O2-induced apoptosis of HASMCs. hasmcs 88-94 phosphatase and tensin homolog pseudogene 1 Homo sapiens 0-6 30144450-6 2018 Ca levels were significantly increased in HASMCs cultured in IFN-gamma-treated medium, compared with non-IFN-gamma-treated medium in the presence of Pi (0.9-2.4 mM). hasmcs 42-48 interferon gamma Homo sapiens 61-70 31210846-7 2019 In addition, overexpression of BOP1 in human aortic smooth muscle cells (HASMCs) inhibited apoptosis and accumulation of p53 under hypoxic conditions, while knockdown of BOP1 decreased the protein synthesis rate and motility of HASMCs. hasmcs 73-79 BOP1 ribosomal biogenesis factor Homo sapiens 31-35 31210846-7 2019 In addition, overexpression of BOP1 in human aortic smooth muscle cells (HASMCs) inhibited apoptosis and accumulation of p53 under hypoxic conditions, while knockdown of BOP1 decreased the protein synthesis rate and motility of HASMCs. hasmcs 73-79 tumor protein p53 Homo sapiens 121-124 31210846-7 2019 In addition, overexpression of BOP1 in human aortic smooth muscle cells (HASMCs) inhibited apoptosis and accumulation of p53 under hypoxic conditions, while knockdown of BOP1 decreased the protein synthesis rate and motility of HASMCs. hasmcs 228-234 BOP1 ribosomal biogenesis factor Homo sapiens 31-35 30816458-7 2019 Autophagy of HASMCs was inhibited following Rab7 knockdown. hasmcs 13-19 RAB7B, member RAS oncogene family Homo sapiens 44-48 30816458-8 2019 Inhibition of autophagy with 3-methyladenine or Rab7 knockdown suppressed the phenotypic conversion of contractile to synthetic HASMCs. hasmcs 128-134 RAB7B, member RAS oncogene family Homo sapiens 48-52 30651873-10 2019 Hyperphosphatemia and hyperglycemia had combined effects in promoting calcification, phenotypic transition and Pit-1 expression in cultured HASMCs. hasmcs 140-146 POU class 1 homeobox 1 Homo sapiens 111-116 30387209-14 2018 These results imply that PGC-1alpha/ERR-alpha played important physiological roles in inhibiting the proliferation of HASMCs by modulating Mfn-2 gene expression. hasmcs 118-124 PPARG coactivator 1 alpha Homo sapiens 25-35 30387209-14 2018 These results imply that PGC-1alpha/ERR-alpha played important physiological roles in inhibiting the proliferation of HASMCs by modulating Mfn-2 gene expression. hasmcs 118-124 estrogen related receptor alpha Homo sapiens 36-45 30387209-14 2018 These results imply that PGC-1alpha/ERR-alpha played important physiological roles in inhibiting the proliferation of HASMCs by modulating Mfn-2 gene expression. hasmcs 118-124 mitofusin 2 Homo sapiens 139-144 30064908-11 2018 These results revealed that LPC was converted into LPA by ATX to induce the proliferation and migration in HASMCs through LPA1/Gi/o/MAP Kinase signaling pathway. hasmcs 107-113 ectonucleotide pyrophosphatase/phosphodiesterase 2 Homo sapiens 58-61 30064908-11 2018 These results revealed that LPC was converted into LPA by ATX to induce the proliferation and migration in HASMCs through LPA1/Gi/o/MAP Kinase signaling pathway. hasmcs 107-113 lysophosphatidic acid receptor 1 Homo sapiens 122-131 30258282-8 2018 Plasma from AD patients further increased Bax mRNA levels but decreased Bcl2 and alpha-SMA mRNA levels in Ang II-treated HASMCs. hasmcs 121-127 BCL2 apoptosis regulator Homo sapiens 72-76 30258282-8 2018 Plasma from AD patients further increased Bax mRNA levels but decreased Bcl2 and alpha-SMA mRNA levels in Ang II-treated HASMCs. hasmcs 121-127 angiotensinogen Homo sapiens 106-112 29143982-3 2018 Compared to HASMCs from non-asthmatic patients, those from asthmatic patients showed elevated expression levels of both Fn14 and TWEAK. hasmcs 12-18 TNF receptor superfamily member 12A Homo sapiens 120-124 29143982-3 2018 Compared to HASMCs from non-asthmatic patients, those from asthmatic patients showed elevated expression levels of both Fn14 and TWEAK. hasmcs 12-18 TNF superfamily member 12 Homo sapiens 129-134 29143982-4 2018 Additionally, similar to the response triggered by platelet-derived growth factor-BB, stimulation with recombinant TWEAK strongly induced cell proliferation and migration in HASMCs. hasmcs 174-180 TNF superfamily member 12 Homo sapiens 115-120 29143982-5 2018 However, depletion of Fn14 remarkably abrogated the enhancement of TWEAK on the cell proliferation and migration of HASMCs. hasmcs 116-122 TNF receptor superfamily member 12A Homo sapiens 22-26 29143982-5 2018 However, depletion of Fn14 remarkably abrogated the enhancement of TWEAK on the cell proliferation and migration of HASMCs. hasmcs 116-122 TNF superfamily member 12 Homo sapiens 67-72 29195902-10 2018 Dickkopf-1 protein, the Wnt/beta-catenin signaling inhibitor, suppressed anti-miR-29b-enhanced HASMCs calcification. hasmcs 95-101 dickkopf WNT signaling pathway inhibitor 1 Homo sapiens 0-10 29195902-10 2018 Dickkopf-1 protein, the Wnt/beta-catenin signaling inhibitor, suppressed anti-miR-29b-enhanced HASMCs calcification. hasmcs 95-101 catenin beta 1 Homo sapiens 28-40 29195902-10 2018 Dickkopf-1 protein, the Wnt/beta-catenin signaling inhibitor, suppressed anti-miR-29b-enhanced HASMCs calcification. hasmcs 95-101 microRNA 29b-1 Homo sapiens 78-85 29330517-7 2018 HASMCs demonstrated calcification induced by iron and TNF-alpha. hasmcs 0-6 tumor necrosis factor Homo sapiens 54-63 29330517-8 2018 Calcification of HASMCs was synergistically enhanced by stimulation with both iron and TNF-alpha. hasmcs 17-23 tumor necrosis factor Homo sapiens 87-96 29330517-10 2018 Stimulation of HASMCs by IL-24 instead of iron induced calcification. hasmcs 15-21 interleukin 24 Homo sapiens 25-30 28816402-6 2017 Blocked the activation of ERK1/2 could decrease S100A12 induced the apoptosis and inhibited cell proliferation of HASMCs. hasmcs 114-120 mitogen-activated protein kinase 3 Homo sapiens 26-32 29403548-4 2018 In addition, miR-31 proliferation was detected by Cell Counting Kit-8 and EdU assays; proliferation was significantly promoted in platelet-derived growth factor (PDGF)-BB-induced human ASMCs (HASMCs) (P<0.001). hasmcs 192-198 microRNA 31 Homo sapiens 13-19 29403548-5 2018 miR-31 migration was detected by transwell and wound closure assays, and was revealed to be promoted in PDGF-BB-induced HASMCs (P<0.001). hasmcs 120-126 microRNA 31 Homo sapiens 0-6 29403548-6 2018 Lastly, HASMCs were transfected with miR-31 mimics and inhibitors, and negative controls. hasmcs 8-14 microRNA 31 Homo sapiens 37-43 29403548-8 2018 These findings suggest that miR-31 is able to promote the proliferation and migration of HASMCs, at least in part, by targeting MFN2. hasmcs 89-95 microRNA 31 Homo sapiens 28-34 29403548-8 2018 These findings suggest that miR-31 is able to promote the proliferation and migration of HASMCs, at least in part, by targeting MFN2. hasmcs 89-95 mitofusin 2 Homo sapiens 128-132 29071730-10 2018 In hASMCs, acute treatment with BK triggered subcellular translocation of ARHGEF1 and RhoA and enhanced auto-phosphorylation of SrcFK and phosphorylation of MYPT1 and MLC20 , but induced de-phosphorylation of cofilin. hasmcs 3-9 kininogen 1 Homo sapiens 32-34 29071730-12 2018 In hASMCs, an ARHGEF1 small interfering RNA suppressed the effects of BK and TGF-beta on RhoA-GTP content, RhoA translocation and MYPT1 and MLC20 phosphorylation, but minimally influenced the effects of TGF-beta on cofilin expression and phosphorylation. hasmcs 3-9 Rho guanine nucleotide exchange factor 1 Homo sapiens 14-21 29071730-12 2018 In hASMCs, an ARHGEF1 small interfering RNA suppressed the effects of BK and TGF-beta on RhoA-GTP content, RhoA translocation and MYPT1 and MLC20 phosphorylation, but minimally influenced the effects of TGF-beta on cofilin expression and phosphorylation. hasmcs 3-9 kininogen 1 Homo sapiens 70-72 29635231-7 2018 OPG was produced and secreted by HASMCs and (to a considerably lesser degree) HAECs under basal conditions. hasmcs 33-39 TNF receptor superfamily member 11b Homo sapiens 0-3 29635231-9 2018 Cyclic strain significantly upregulated OPG production in HASMCs. hasmcs 58-64 TNF receptor superfamily member 11b Homo sapiens 40-43 29635231-11 2018 Exposing HASMCs to exogenous RANKL inhibited basal OPG production and completely abrogated the strain-mediated upregulation of OPG. hasmcs 9-15 TNF superfamily member 11 Homo sapiens 29-34 29635231-11 2018 Exposing HASMCs to exogenous RANKL inhibited basal OPG production and completely abrogated the strain-mediated upregulation of OPG. hasmcs 9-15 TNF receptor superfamily member 11b Homo sapiens 51-54 29635231-11 2018 Exposing HASMCs to exogenous RANKL inhibited basal OPG production and completely abrogated the strain-mediated upregulation of OPG. hasmcs 9-15 TNF receptor superfamily member 11b Homo sapiens 127-130 29635231-12 2018 These data suggest that HASMCs are a significant source of OPG within the vasculature but that RANKL, once present, downregulates this production and appears capable of preventing the "protective" upregulation of OPG seen with HASMCs exposed to physiological levels of cyclic strain. hasmcs 227-233 TNF superfamily member 11 Homo sapiens 95-100 29635231-12 2018 These data suggest that HASMCs are a significant source of OPG within the vasculature but that RANKL, once present, downregulates this production and appears capable of preventing the "protective" upregulation of OPG seen with HASMCs exposed to physiological levels of cyclic strain. hasmcs 227-233 TNF receptor superfamily member 11b Homo sapiens 213-216 28816402-6 2017 Blocked the activation of ERK1/2 could decrease S100A12 induced the apoptosis and inhibited cell proliferation of HASMCs. hasmcs 114-120 S100 calcium binding protein A12 Homo sapiens 48-55 28816402-7 2017 In conclusion, these results indicated that S100A12 could increase the expression of MMP-2, MMP-9, and vascular cell adhesion molecule 1 (VCAM-1) in HASMCs via activation of ERK1/2 signal pathway, which leads to injury of HASMCs. hasmcs 149-155 S100 calcium binding protein A12 Homo sapiens 44-51 28816402-7 2017 In conclusion, these results indicated that S100A12 could increase the expression of MMP-2, MMP-9, and vascular cell adhesion molecule 1 (VCAM-1) in HASMCs via activation of ERK1/2 signal pathway, which leads to injury of HASMCs. hasmcs 149-155 matrix metallopeptidase 2 Homo sapiens 85-90 28816402-7 2017 In conclusion, these results indicated that S100A12 could increase the expression of MMP-2, MMP-9, and vascular cell adhesion molecule 1 (VCAM-1) in HASMCs via activation of ERK1/2 signal pathway, which leads to injury of HASMCs. hasmcs 149-155 matrix metallopeptidase 9 Homo sapiens 92-97 28816402-7 2017 In conclusion, these results indicated that S100A12 could increase the expression of MMP-2, MMP-9, and vascular cell adhesion molecule 1 (VCAM-1) in HASMCs via activation of ERK1/2 signal pathway, which leads to injury of HASMCs. hasmcs 149-155 vascular cell adhesion molecule 1 Homo sapiens 103-136 28816402-7 2017 In conclusion, these results indicated that S100A12 could increase the expression of MMP-2, MMP-9, and vascular cell adhesion molecule 1 (VCAM-1) in HASMCs via activation of ERK1/2 signal pathway, which leads to injury of HASMCs. hasmcs 149-155 vascular cell adhesion molecule 1 Homo sapiens 138-144 28816402-7 2017 In conclusion, these results indicated that S100A12 could increase the expression of MMP-2, MMP-9, and vascular cell adhesion molecule 1 (VCAM-1) in HASMCs via activation of ERK1/2 signal pathway, which leads to injury of HASMCs. hasmcs 149-155 mitogen-activated protein kinase 3 Homo sapiens 174-180 28551013-5 2017 Pretreatment of HASMCs with nomilin stimulated extrinsic caspase-8, intrinsic caspase-9, and apoptotic caspase-3 and resulted in significant inhibition of TNF-alpha-induced proliferation. hasmcs 16-22 caspase 8 Homo sapiens 57-66 29145460-9 2017 Importantly, several RANKL actions (e.g. increased BMP-2 release from mono-cultured HAECs or increased ALP/Sox9 levels in co-cultured HASMCs) could be strongly blocked by co-incubation with TRAIL. hasmcs 134-140 TNF superfamily member 11 Homo sapiens 21-26 29145460-9 2017 Importantly, several RANKL actions (e.g. increased BMP-2 release from mono-cultured HAECs or increased ALP/Sox9 levels in co-cultured HASMCs) could be strongly blocked by co-incubation with TRAIL. hasmcs 134-140 ATHS Homo sapiens 103-106 29145460-9 2017 Importantly, several RANKL actions (e.g. increased BMP-2 release from mono-cultured HAECs or increased ALP/Sox9 levels in co-cultured HASMCs) could be strongly blocked by co-incubation with TRAIL. hasmcs 134-140 SRY-box transcription factor 9 Homo sapiens 107-111 29145460-9 2017 Importantly, several RANKL actions (e.g. increased BMP-2 release from mono-cultured HAECs or increased ALP/Sox9 levels in co-cultured HASMCs) could be strongly blocked by co-incubation with TRAIL. hasmcs 134-140 TNF superfamily member 10 Homo sapiens 190-195 28551013-5 2017 Pretreatment of HASMCs with nomilin stimulated extrinsic caspase-8, intrinsic caspase-9, and apoptotic caspase-3 and resulted in significant inhibition of TNF-alpha-induced proliferation. hasmcs 16-22 caspase 9 Homo sapiens 78-87 28551013-5 2017 Pretreatment of HASMCs with nomilin stimulated extrinsic caspase-8, intrinsic caspase-9, and apoptotic caspase-3 and resulted in significant inhibition of TNF-alpha-induced proliferation. hasmcs 16-22 caspase 3 Homo sapiens 103-112 28551013-5 2017 Pretreatment of HASMCs with nomilin stimulated extrinsic caspase-8, intrinsic caspase-9, and apoptotic caspase-3 and resulted in significant inhibition of TNF-alpha-induced proliferation. hasmcs 16-22 tumor necrosis factor Homo sapiens 155-164 28551013-7 2017 Furthermore, nomilin significantly decreased the phosphorylation of IkappaBalpha, an inhibitor of NF-kappaB and subsequently, reduced the downstream inflammatory signaling in TNF-alpha treated HASMCs. hasmcs 193-199 NFKB inhibitor alpha Homo sapiens 68-80 28551013-7 2017 Furthermore, nomilin significantly decreased the phosphorylation of IkappaBalpha, an inhibitor of NF-kappaB and subsequently, reduced the downstream inflammatory signaling in TNF-alpha treated HASMCs. hasmcs 193-199 tumor necrosis factor Homo sapiens 175-184 28551013-8 2017 Our findings indicate that the anti-proliferative activity of nomilin on TNF-alpha-induced HASMCs results from apoptosis through a mitochondrial-dependent pathway and suppression of inflammatory signaling mediated through NF-kappaB. hasmcs 91-97 tumor necrosis factor Homo sapiens 73-82 27540365-5 2016 Using fluorometric calcium imaging, specific agonists for OR2AG1 and OR1D2 were identified to trigger transient Ca(2+) increases in HASMCs via a cAMP-dependent signal transduction cascade. hasmcs 132-138 olfactory receptor family 2 subfamily AG member 1 Homo sapiens 58-64 28698179-6 2017 In the cytoplasm of HASMCs (human aortic smooth muscle cells), the NRG-1-ICD participated in filamentous actin formation by interacting with alpha-SMA (smooth muscle alpha-actin). hasmcs 20-26 neuregulin 1 Homo sapiens 67-72 28292711-4 2017 Ang II significantly up-regulated MMP-9 expression and cell migration of HASMCs, which was inhibited by transfection with siRNA of p47phox, Nox2, Nox4, p65, angiotensin II type 1 receptor (AT1R) and pretreatment with the inhibitors of NADPH oxidase, ROS, and NF-kappaB. hasmcs 73-79 angiogenin Homo sapiens 0-3 28292711-4 2017 Ang II significantly up-regulated MMP-9 expression and cell migration of HASMCs, which was inhibited by transfection with siRNA of p47phox, Nox2, Nox4, p65, angiotensin II type 1 receptor (AT1R) and pretreatment with the inhibitors of NADPH oxidase, ROS, and NF-kappaB. hasmcs 73-79 matrix metallopeptidase 9 Homo sapiens 34-39 28292711-4 2017 Ang II significantly up-regulated MMP-9 expression and cell migration of HASMCs, which was inhibited by transfection with siRNA of p47phox, Nox2, Nox4, p65, angiotensin II type 1 receptor (AT1R) and pretreatment with the inhibitors of NADPH oxidase, ROS, and NF-kappaB. hasmcs 73-79 neutrophil cytosolic factor 1 Homo sapiens 131-138 28292711-4 2017 Ang II significantly up-regulated MMP-9 expression and cell migration of HASMCs, which was inhibited by transfection with siRNA of p47phox, Nox2, Nox4, p65, angiotensin II type 1 receptor (AT1R) and pretreatment with the inhibitors of NADPH oxidase, ROS, and NF-kappaB. hasmcs 73-79 cytochrome b-245 beta chain Homo sapiens 140-144 28292711-4 2017 Ang II significantly up-regulated MMP-9 expression and cell migration of HASMCs, which was inhibited by transfection with siRNA of p47phox, Nox2, Nox4, p65, angiotensin II type 1 receptor (AT1R) and pretreatment with the inhibitors of NADPH oxidase, ROS, and NF-kappaB. hasmcs 73-79 NADPH oxidase 4 Homo sapiens 146-150 28292711-4 2017 Ang II significantly up-regulated MMP-9 expression and cell migration of HASMCs, which was inhibited by transfection with siRNA of p47phox, Nox2, Nox4, p65, angiotensin II type 1 receptor (AT1R) and pretreatment with the inhibitors of NADPH oxidase, ROS, and NF-kappaB. hasmcs 73-79 RELA proto-oncogene, NF-kB subunit Homo sapiens 152-155 28292711-4 2017 Ang II significantly up-regulated MMP-9 expression and cell migration of HASMCs, which was inhibited by transfection with siRNA of p47phox, Nox2, Nox4, p65, angiotensin II type 1 receptor (AT1R) and pretreatment with the inhibitors of NADPH oxidase, ROS, and NF-kappaB. hasmcs 73-79 angiotensin II receptor type 1 Homo sapiens 157-187 28292711-4 2017 Ang II significantly up-regulated MMP-9 expression and cell migration of HASMCs, which was inhibited by transfection with siRNA of p47phox, Nox2, Nox4, p65, angiotensin II type 1 receptor (AT1R) and pretreatment with the inhibitors of NADPH oxidase, ROS, and NF-kappaB. hasmcs 73-79 angiotensin II receptor type 1 Homo sapiens 189-193 28716056-8 2017 Cell cycle of ISL treated HASMCs arrested mainly in G1/S phase and accompanied with elevated expression of p27 and decreased expression of CyclinD1 and CyclinE. hasmcs 26-32 zinc ribbon domain containing 2 Homo sapiens 107-110 28716056-8 2017 Cell cycle of ISL treated HASMCs arrested mainly in G1/S phase and accompanied with elevated expression of p27 and decreased expression of CyclinD1 and CyclinE. hasmcs 26-32 cyclin D1 Homo sapiens 139-147 28716056-9 2017 In addition, ISL could down-regulated the expression of p-PI3K and p-AKT, alleviated oxidative stress and enhanced the SOD activity in HASMCs. hasmcs 135-141 superoxide dismutase 1 Homo sapiens 119-122 28716056-10 2017 Furthermore, H2O2 treatment partly improved cell viability and up regulated p-PI3K and p-AKT in HASMCs. hasmcs 96-102 AKT serine/threonine kinase 1 Homo sapiens 89-92 28716056-11 2017 CONCLUSIONS: Therefore, we concluded that ISL inhibited the proliferation of HASMCs via attenuating oxidative stress and suppressing PI3K/AKT signaling pathway. hasmcs 77-83 AKT serine/threonine kinase 1 Homo sapiens 138-141 28672917-8 2017 Overexpression of P16 inhibited CSE-induced cell proliferation through inducing cell cycle arrest in G1 phase (P<0.001), and led to decreased levels of CDK4 (P<0.01), CDK6 (P<0.01) and p-Rb (P<0.001) in HASMCs. hasmcs 215-221 cyclin dependent kinase inhibitor 2A Homo sapiens 18-21 28415624-5 2017 Mechanically, hypoxia promoted the expression of HIF-1a by PI3K-AKT pathway in HASMCs; HIF-1a further suppressed the expressions of AEG-1, a-SMA and SM22a, and promoted osteopontin (OPN) expression. hasmcs 79-85 hypoxia inducible factor 1 subunit alpha Homo sapiens 49-55 28415624-5 2017 Mechanically, hypoxia promoted the expression of HIF-1a by PI3K-AKT pathway in HASMCs; HIF-1a further suppressed the expressions of AEG-1, a-SMA and SM22a, and promoted osteopontin (OPN) expression. hasmcs 79-85 AKT serine/threonine kinase 1 Homo sapiens 64-67 28173642-5 2017 In primary human aortic SMCs (HASMCs), UII (50 nM) induced proliferation was significantly inhibited by KR-36996 at 1, 10, and 100 nM which showed greater potency (IC50: 3.5 nM) than GSK-1440115 (IC50: 82.3 nM). hasmcs 30-36 urotensin 2 Homo sapiens 39-42 28062540-8 2017 In vitro, knockdown of T-cadherin from human aortic smooth muscle cells (HASMCs) with synthetic phenotype significantly reduced adiponectin accumulation on HASMCs and negated the inhibitory effect of adiponectin on proinflammatory change. hasmcs 73-79 cadherin 13 Homo sapiens 23-33 28062540-8 2017 In vitro, knockdown of T-cadherin from human aortic smooth muscle cells (HASMCs) with synthetic phenotype significantly reduced adiponectin accumulation on HASMCs and negated the inhibitory effect of adiponectin on proinflammatory change. hasmcs 73-79 adiponectin, C1Q and collagen domain containing Homo sapiens 128-139 28062540-8 2017 In vitro, knockdown of T-cadherin from human aortic smooth muscle cells (HASMCs) with synthetic phenotype significantly reduced adiponectin accumulation on HASMCs and negated the inhibitory effect of adiponectin on proinflammatory change. hasmcs 73-79 adiponectin, C1Q and collagen domain containing Homo sapiens 200-211 28062540-8 2017 In vitro, knockdown of T-cadherin from human aortic smooth muscle cells (HASMCs) with synthetic phenotype significantly reduced adiponectin accumulation on HASMCs and negated the inhibitory effect of adiponectin on proinflammatory change. hasmcs 156-162 cadherin 13 Homo sapiens 23-33 28062540-8 2017 In vitro, knockdown of T-cadherin from human aortic smooth muscle cells (HASMCs) with synthetic phenotype significantly reduced adiponectin accumulation on HASMCs and negated the inhibitory effect of adiponectin on proinflammatory change. hasmcs 156-162 adiponectin, C1Q and collagen domain containing Homo sapiens 128-139 27766038-6 2016 The expression of DNA methyltransferase (DNMT) 1 and 3a in HASMCs treated with IS was significantly increased and specific inhibition of DNA methyltransferase 1 by 5-aza-2"-deoxycytidine(5Aza-2dc) caused demethylation of the Klotho gene and increased Klotho expression. hasmcs 59-65 DNA methyltransferase 1 Homo sapiens 18-54 27766038-6 2016 The expression of DNA methyltransferase (DNMT) 1 and 3a in HASMCs treated with IS was significantly increased and specific inhibition of DNA methyltransferase 1 by 5-aza-2"-deoxycytidine(5Aza-2dc) caused demethylation of the Klotho gene and increased Klotho expression. hasmcs 59-65 DNA methyltransferase 1 Homo sapiens 137-160 27766038-6 2016 The expression of DNA methyltransferase (DNMT) 1 and 3a in HASMCs treated with IS was significantly increased and specific inhibition of DNA methyltransferase 1 by 5-aza-2"-deoxycytidine(5Aza-2dc) caused demethylation of the Klotho gene and increased Klotho expression. hasmcs 59-65 klotho Homo sapiens 225-231 27766038-6 2016 The expression of DNA methyltransferase (DNMT) 1 and 3a in HASMCs treated with IS was significantly increased and specific inhibition of DNA methyltransferase 1 by 5-aza-2"-deoxycytidine(5Aza-2dc) caused demethylation of the Klotho gene and increased Klotho expression. hasmcs 59-65 klotho Homo sapiens 251-257 27353340-12 2016 Furthermore, downregulation of KLF5 significantly alleviated the effects of Oct4 on phenotype transition of HASMCs. hasmcs 108-114 Kruppel like factor 5 Homo sapiens 31-35 27353340-12 2016 Furthermore, downregulation of KLF5 significantly alleviated the effects of Oct4 on phenotype transition of HASMCs. hasmcs 108-114 POU class 5 homeobox 1 Homo sapiens 76-80 27353340-14 2016 The increased Oct4 induced phenotype transition of HASMCs from the contractile type to the synthetic type by directly upregulating KLF5. hasmcs 51-57 POU class 5 homeobox 1 Homo sapiens 14-18 27353340-14 2016 The increased Oct4 induced phenotype transition of HASMCs from the contractile type to the synthetic type by directly upregulating KLF5. hasmcs 51-57 Kruppel like factor 5 Homo sapiens 131-135 27540365-5 2016 Using fluorometric calcium imaging, specific agonists for OR2AG1 and OR1D2 were identified to trigger transient Ca(2+) increases in HASMCs via a cAMP-dependent signal transduction cascade. hasmcs 132-138 olfactory receptor family 1 subfamily D member 2 Homo sapiens 69-74 27540365-6 2016 Furthermore, the activation of OR2AG1 via amyl butyrate inhibited the histamine-induced contraction of HASMCs, whereas the stimulation of OR1D2 with bourgeonal led to an increase in cell contractility. hasmcs 103-109 olfactory receptor family 2 subfamily AG member 1 Homo sapiens 31-37 27035566-7 2016 Furthermore, siRNA-SCARA5 significantly inhibited the phosphorylation of PDGF receptor (PDGFR) beta, AKT and extracellular signal-regulated kinase 1/2 in PDGF-BB-stimulated HASMCs. hasmcs 173-179 scavenger receptor class A member 5 Homo sapiens 19-25 27035566-7 2016 Furthermore, siRNA-SCARA5 significantly inhibited the phosphorylation of PDGF receptor (PDGFR) beta, AKT and extracellular signal-regulated kinase 1/2 in PDGF-BB-stimulated HASMCs. hasmcs 173-179 platelet derived growth factor receptor beta Homo sapiens 73-99 27035566-7 2016 Furthermore, siRNA-SCARA5 significantly inhibited the phosphorylation of PDGF receptor (PDGFR) beta, AKT and extracellular signal-regulated kinase 1/2 in PDGF-BB-stimulated HASMCs. hasmcs 173-179 AKT serine/threonine kinase 1 Homo sapiens 101-104 27035566-7 2016 Furthermore, siRNA-SCARA5 significantly inhibited the phosphorylation of PDGF receptor (PDGFR) beta, AKT and extracellular signal-regulated kinase 1/2 in PDGF-BB-stimulated HASMCs. hasmcs 173-179 mitogen-activated protein kinase 3 Homo sapiens 109-150 27120457-9 2016 Lower NF-kappaB nuclear import rates were observed when JNK or TRAF6 silenced HASMCs were stimulated with CD40L compared to HASMCs with active JNK or TRAF6. hasmcs 78-84 mitogen-activated protein kinase 8 Homo sapiens 56-59 27120457-9 2016 Lower NF-kappaB nuclear import rates were observed when JNK or TRAF6 silenced HASMCs were stimulated with CD40L compared to HASMCs with active JNK or TRAF6. hasmcs 78-84 TNF receptor associated factor 6 Homo sapiens 63-68 27120457-9 2016 Lower NF-kappaB nuclear import rates were observed when JNK or TRAF6 silenced HASMCs were stimulated with CD40L compared to HASMCs with active JNK or TRAF6. hasmcs 78-84 CD40 ligand Homo sapiens 106-111 26783147-5 2016 The results showed that matrine inhibited the expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) in TNF-alpha-stimulated HASMCs. hasmcs 174-180 vascular cell adhesion molecule 1 Homo sapiens 60-93 26828753-9 2016 Fetuin-A enhanced macrophage foam cell formation associated with scavenger receptors (CD36 and SR-A) and acyl-CoA:cholesterol acyltransferase-1 up-regulation and ATP-binding cassette transporter A1 down-regulation, and increased cell proliferation and collagen-1 and -3 expression via PI3K/AKT/c-Src/NF-kappaB/ERK1/2 pathways in HASMCs. hasmcs 329-335 alpha 2-HS glycoprotein Homo sapiens 0-8 26783147-5 2016 The results showed that matrine inhibited the expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) in TNF-alpha-stimulated HASMCs. hasmcs 174-180 vascular cell adhesion molecule 1 Homo sapiens 95-101 26783147-5 2016 The results showed that matrine inhibited the expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) in TNF-alpha-stimulated HASMCs. hasmcs 174-180 intercellular adhesion molecule 1 Homo sapiens 107-140 26783147-5 2016 The results showed that matrine inhibited the expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) in TNF-alpha-stimulated HASMCs. hasmcs 174-180 intercellular adhesion molecule 1 Homo sapiens 142-148 26783147-5 2016 The results showed that matrine inhibited the expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) in TNF-alpha-stimulated HASMCs. hasmcs 174-180 tumor necrosis factor Homo sapiens 153-162 26783147-7 2016 Furthermore, matrine inhibited the production of intracellular reactive oxygen species (ROS) in TNF-alpha-stimulated HASMCs. hasmcs 117-123 tumor necrosis factor Homo sapiens 96-105 26783147-8 2016 In conclusion, the results of the present study demonstrated that matrine inhibited the expression of VCAM-1 and ICAM-1 in TNF-alpha-stimulated HASMCs via the suppression of ROS production as well as NF-kappaB and MAPK pathway activation. hasmcs 144-150 vascular cell adhesion molecule 1 Homo sapiens 102-108 26783147-8 2016 In conclusion, the results of the present study demonstrated that matrine inhibited the expression of VCAM-1 and ICAM-1 in TNF-alpha-stimulated HASMCs via the suppression of ROS production as well as NF-kappaB and MAPK pathway activation. hasmcs 144-150 intercellular adhesion molecule 1 Homo sapiens 113-119 26783147-8 2016 In conclusion, the results of the present study demonstrated that matrine inhibited the expression of VCAM-1 and ICAM-1 in TNF-alpha-stimulated HASMCs via the suppression of ROS production as well as NF-kappaB and MAPK pathway activation. hasmcs 144-150 tumor necrosis factor Homo sapiens 123-132 26608913-3 2016 We hypothesized that HLMC and human ASM cell (HASMC) responsiveness to beta2-AR agonists would be attenuated when HLMCs are in contact with HASMCs. hasmcs 140-146 adrenoceptor beta 2 Homo sapiens 71-79 26581047-3 2016 We determined whether apelin plays a role in phosphate-induced mineralization of human aortic smooth muscle cells (HASMCs) and in adenine-induced CKD rats with aortic calcification. hasmcs 115-121 apelin Homo sapiens 22-28 26581047-4 2016 METHODS AND RESULTS: In vitro, apelin-13 was found to inhibit calcium deposition in HASMCs (Pi(+) Apelin(+) group vs Pi(+) Apelin(-) group: 50.1 +- 6.21 ug/mg vs 146.67 +- 10.02 ug/mg protein, p = 0.012) and to suppress the induction of the osteoblastic transformation genes BMP-2, osteoprotegerin (OPG) and Cbfa1. hasmcs 84-90 apelin Rattus norvegicus 31-37 26608913-10 2016 beta2-AR phosphorylation at Tyr(350) occurred within 5 min in both HLMCs and HASMCs when the cells were cocultured, and was inhibited by neutralizing SCF or CADM1. hasmcs 77-83 adrenoceptor beta 2 Homo sapiens 0-8 26608913-10 2016 beta2-AR phosphorylation at Tyr(350) occurred within 5 min in both HLMCs and HASMCs when the cells were cocultured, and was inhibited by neutralizing SCF or CADM1. hasmcs 77-83 KIT ligand Homo sapiens 150-153 26608913-11 2016 HLMC interactions with HASMCs via CADM1 and Kit inhibit the potentially beneficial effects of beta2-AR agonists on these cells via phosphorylation of the beta2-AR. hasmcs 23-29 cell adhesion molecule 1 Homo sapiens 34-39 26608913-11 2016 HLMC interactions with HASMCs via CADM1 and Kit inhibit the potentially beneficial effects of beta2-AR agonists on these cells via phosphorylation of the beta2-AR. hasmcs 23-29 adrenoceptor beta 2 Homo sapiens 94-102 26608913-11 2016 HLMC interactions with HASMCs via CADM1 and Kit inhibit the potentially beneficial effects of beta2-AR agonists on these cells via phosphorylation of the beta2-AR. hasmcs 23-29 adrenoceptor beta 2 Homo sapiens 154-162 26385608-2 2016 In this study, we examined the potencies of other vitamin D3 and D2 analogs to stimulate the ectodomain shedding of TNFR1 in HASMCs. hasmcs 125-131 TNF receptor superfamily member 1A Homo sapiens 116-121 26502995-5 2015 Furthermore, the activation of the extracellular signal-regulated protein kinase (ERK) signaling pathway was evaluated in HASMCs. hasmcs 122-128 mitogen-activated protein kinase 1 Homo sapiens 35-80 27352232-10 2016 Ang-(1-7) suppressed phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) and NF-x03BA;B in HASMCs. hasmcs 109-115 mitogen activated protein kinase 3 Rattus norvegicus 40-81 26487003-10 2015 We conclude that 2-ME inhibits the pathway RhoA ROCK1 myosin phosphatase targeting subunit myosin light chain, and this likely contributes to the reduced cytokinesis in 2-ME treated HASMCs. hasmcs 188-194 ras homolog family member A Homo sapiens 43-47 26487003-10 2015 We conclude that 2-ME inhibits the pathway RhoA ROCK1 myosin phosphatase targeting subunit myosin light chain, and this likely contributes to the reduced cytokinesis in 2-ME treated HASMCs. hasmcs 188-194 Rho associated coiled-coil containing protein kinase 1 Homo sapiens 50-55 26577834-7 2015 (i) In cultured HASMCs, the resting pHi was 7.19 +- 0.04 and 7.13 +- 0.02 for HEPES- and CO2 /HCO3--buffered solution, respectively. hasmcs 16-22 glucose-6-phosphate isomerase Homo sapiens 36-39 26502995-5 2015 Furthermore, the activation of the extracellular signal-regulated protein kinase (ERK) signaling pathway was evaluated in HASMCs. hasmcs 122-128 mitogen-activated protein kinase 1 Homo sapiens 82-85 26077572-7 2015 Angiotensin II inhibits TMEM16A expression via Kruppel-like factor 5 (KLF5) in cultured HASMCs. hasmcs 88-94 angiotensinogen Rattus norvegicus 0-14 26077572-7 2015 Angiotensin II inhibits TMEM16A expression via Kruppel-like factor 5 (KLF5) in cultured HASMCs. hasmcs 88-94 anoctamin 1, calcium activated chloride channel Mus musculus 24-31 26077572-7 2015 Angiotensin II inhibits TMEM16A expression via Kruppel-like factor 5 (KLF5) in cultured HASMCs. hasmcs 88-94 Kruppel-like factor 5 Mus musculus 47-68 26077572-7 2015 Angiotensin II inhibits TMEM16A expression via Kruppel-like factor 5 (KLF5) in cultured HASMCs. hasmcs 88-94 Kruppel-like factor 5 Mus musculus 70-74 25872580-6 2015 We also demonstrated that the exogenous overexpression of miR-155 significantly enhanced cell proliferation by inhibiting apoptosis in human aortic SMCs (HASMCs), and it also promoted the migratory ability of the cells. hasmcs 154-160 microRNA 155 Homo sapiens 58-65 26174316-5 2015 METHODS: We investigated the effect of Illicium v. on cytotoxicity, NF-kappaB activity, and adhesion molecule expression in TNF-alpha--stimulated HASMCs (Human Aortic smooth muscle cells). hasmcs 146-152 tumor necrosis factor Mus musculus 124-133 26174316-8 2015 RESULTS: In TNF-alpha-stimulated HASMCs, Illicium v. treatment decreased NF-kappaB transcriptional activity, and NF-kappaB protein levels were reduced in a dose-dependent manner over a range of 10-100 mug/mL Illicium v. Also, Illicium v. attenuated the expression of adhesion molecules that are responsible for inflammation in these cells. hasmcs 33-39 tumor necrosis factor Mus musculus 12-21 26198965-2 2015 METHODS: HASMCs were treated with 10-100 nmol/L anti-miR-145, and the cell proliferation and apoptosis were investigated using a CCK-8 assay and flow cytometry, respectively. hasmcs 9-15 microRNA 145 Homo sapiens 53-60 26198965-4 2015 RESULTS: Treatment with 10 and 50 nmol/L anti-miR-145 significantly promoted the proliferation and osteopontin synthesis in HASMCs (P<0.05 or <0.01), and 50 nmol/L anti-miR-145 obviously inhibited the cell apoptosis (P<0.01). hasmcs 124-130 microRNA 145 Homo sapiens 46-53 26198965-4 2015 RESULTS: Treatment with 10 and 50 nmol/L anti-miR-145 significantly promoted the proliferation and osteopontin synthesis in HASMCs (P<0.05 or <0.01), and 50 nmol/L anti-miR-145 obviously inhibited the cell apoptosis (P<0.01). hasmcs 124-130 secreted phosphoprotein 1 Homo sapiens 99-110 25923882-5 2015 MTT was used to test the effect of sTNFR1-IgGFc to antagonism TNF-alpha-induced proliferates of HASMCs. hasmcs 96-102 tumor necrosis factor Mus musculus 62-71 26023787-11 2015 Furthermore, endosomes from asthmatic HASMCs are associated with significant increase in PI3Kgamma and reduced PP2A activity that inhibits beta2AR resensitization. hasmcs 38-44 phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma Homo sapiens 89-98 26023787-11 2015 Furthermore, endosomes from asthmatic HASMCs are associated with significant increase in PI3Kgamma and reduced PP2A activity that inhibits beta2AR resensitization. hasmcs 38-44 protein phosphatase 2 phosphatase activator Homo sapiens 111-115 26023787-11 2015 Furthermore, endosomes from asthmatic HASMCs are associated with significant increase in PI3Kgamma and reduced PP2A activity that inhibits beta2AR resensitization. hasmcs 38-44 adrenoceptor beta 2 Homo sapiens 139-146 25180620-3 2015 We hypothesized that COX-2 expression and PGE2 secretion might be elevated in asthmatic hASMCs in response to proinflammatory signals in part due to altered histone acetylation and/or microRNA expression. hasmcs 88-94 prostaglandin-endoperoxide synthase 2 Homo sapiens 21-26 24682435-2 2015 Real-time PCR, western blots and immunofluorescence were used to determine the expression of GABABR1 and GABABR2 in cultured HASMCs. hasmcs 125-131 gamma-aminobutyric acid type B receptor subunit 1 Homo sapiens 93-100 25716870-8 2015 Four major acetophenones were purified from the ethyl acetate fraction, and two components, p-hydroxyacetophenone and cynandione A, potently inhibited the expression of ICAM-1 and VCAM-1 in the stimulated HASMCs. hasmcs 205-211 intercellular adhesion molecule 1 Mus musculus 169-175 25716870-8 2015 Four major acetophenones were purified from the ethyl acetate fraction, and two components, p-hydroxyacetophenone and cynandione A, potently inhibited the expression of ICAM-1 and VCAM-1 in the stimulated HASMCs. hasmcs 205-211 vascular cell adhesion molecule 1 Mus musculus 180-186 24682435-2 2015 Real-time PCR, western blots and immunofluorescence were used to determine the expression of GABABR1 and GABABR2 in cultured HASMCs. hasmcs 125-131 gamma-aminobutyric acid type B receptor subunit 2 Homo sapiens 105-112 26488172-7 2015 RESULTS: Lv-GFP-sPLA2-IIA-transduced HASMCs remained fluorescent during 72 h of the study period with infection ratio of around 80%. hasmcs 37-43 phospholipase A2 group IIA Homo sapiens 16-21 25651915-5 2015 RESULTS: Our results indicate that SSeo treatment has an inhibitory effect on activation as well as expression of MMP-9 induced by TNF-alpha in HASMCs in a dose-dependent manner without significant cytotoxicity. hasmcs 144-150 matrix metallopeptidase 9 Homo sapiens 114-119 25651915-5 2015 RESULTS: Our results indicate that SSeo treatment has an inhibitory effect on activation as well as expression of MMP-9 induced by TNF-alpha in HASMCs in a dose-dependent manner without significant cytotoxicity. hasmcs 144-150 tumor necrosis factor Homo sapiens 131-140 25529449-9 2015 Statins suppressed migration and proliferation of HASMCs, and inhibited lipopolysaccharide-induced expression of monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-alpha (TNF-alpha) in HASMCs. hasmcs 203-209 C-C motif chemokine ligand 2 Homo sapiens 113-147 25529449-9 2015 Statins suppressed migration and proliferation of HASMCs, and inhibited lipopolysaccharide-induced expression of monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-alpha (TNF-alpha) in HASMCs. hasmcs 203-209 C-C motif chemokine ligand 2 Homo sapiens 149-154 25529449-9 2015 Statins suppressed migration and proliferation of HASMCs, and inhibited lipopolysaccharide-induced expression of monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-alpha (TNF-alpha) in HASMCs. hasmcs 203-209 tumor necrosis factor Homo sapiens 160-187 25529449-9 2015 Statins suppressed migration and proliferation of HASMCs, and inhibited lipopolysaccharide-induced expression of monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-alpha (TNF-alpha) in HASMCs. hasmcs 203-209 tumor necrosis factor Homo sapiens 189-198 24626840-3 2014 To elucidate the role of ZIPK in HG-treated HASMCs, we overexpressed ZIPK by lentivirus infection and knocked down ZIPK by gene deletion using ZIPK shRNA. hasmcs 44-50 death associated protein kinase 3 Homo sapiens 25-29 24626840-7 2014 In conclusion, the results suggested that ZIPK plays a role in HG-treated HASMCs, indicating ZIPK is a potential therapeutic target for the treatment of diabetic vascular complications. hasmcs 74-80 death associated protein kinase 3 Homo sapiens 42-46 24626840-7 2014 In conclusion, the results suggested that ZIPK plays a role in HG-treated HASMCs, indicating ZIPK is a potential therapeutic target for the treatment of diabetic vascular complications. hasmcs 74-80 death associated protein kinase 3 Homo sapiens 93-97 23981542-10 2014 Resveratrol was superior to dexamethasone in reducing CCL-2, IL-6 and IL-8 in LTA-exposed HASMCs of patients with COPD. hasmcs 90-96 C-X-C motif chemokine ligand 8 Homo sapiens 70-74 24880525-10 2014 These results suggest that EGCG inhibits the proliferation of HASMCs in vitro largely via Mfn-2-mediated suppression of the Ras-Raf-ERK/MAPK signaling pathway. hasmcs 62-68 mitofusin 2 Homo sapiens 90-95 24880525-10 2014 These results suggest that EGCG inhibits the proliferation of HASMCs in vitro largely via Mfn-2-mediated suppression of the Ras-Raf-ERK/MAPK signaling pathway. hasmcs 62-68 zinc fingers and homeoboxes 2 Homo sapiens 128-131 24880525-10 2014 These results suggest that EGCG inhibits the proliferation of HASMCs in vitro largely via Mfn-2-mediated suppression of the Ras-Raf-ERK/MAPK signaling pathway. hasmcs 62-68 mitogen-activated protein kinase 1 Homo sapiens 132-135 24363205-9 2014 PDGF-BB-induced migration, F-actin reduction by HASMCs, and ex vivo aortic sprouting were all inhibited by treatment with a commercial FAK inhibitor, PF-228. hasmcs 48-54 protein tyrosine kinase 2 Rattus norvegicus 135-138 23981542-16 2014 Resveratrol and corticosteroids suppress cytokine/chemokine expression through activation of SIRT1 or interaction with class I/II HDACs, respectively, in HASMCs. hasmcs 154-160 sirtuin 1 Homo sapiens 93-98 23932461-9 2014 Sema3E increased plexinD1 expression in HASMCs from asthmatic patients. hasmcs 40-46 semaphorin 3E Homo sapiens 0-6 23932461-9 2014 Sema3E increased plexinD1 expression in HASMCs from asthmatic patients. hasmcs 40-46 plexin D1 Homo sapiens 17-25 25386047-6 2014 The HO-1 inducer CoPPIX decreased IL-1beta-induced cell proliferation, whereas the HO-1 enzyme inhibitor ZnPPIX significantly reversed EGCG-caused growth inhibition in IL-1beta-treated HASMCs. hasmcs 185-191 heme oxygenase 1 Homo sapiens 83-87 25012146-4 2014 Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to measure the expression of miR-205 in HASMCs. hasmcs 120-126 microRNA 205 Homo sapiens 109-116 25012146-5 2014 RESULTS: The expression of endogenous miR-205 was decreased in HASMCs during beta-glycerophosphate-induced calcification. hasmcs 63-69 microRNA 205 Homo sapiens 38-45 24723958-3 2014 The lipopolysaccharide- (LPS-) induced IL-1 beta expression was significantly reduced when HASMCs were pretreated with EORP by Western blot and immunofluorescent staining. hasmcs 91-97 interleukin 1 beta Mus musculus 39-48 24723958-6 2014 In addition, EORP reduced the phosphorylation and nuclear translocation of nuclear factor- (NF-) kappa B p65 in LPS-treated HASMCs. hasmcs 124-130 nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 Mus musculus 75-104 24723958-6 2014 In addition, EORP reduced the phosphorylation and nuclear translocation of nuclear factor- (NF-) kappa B p65 in LPS-treated HASMCs. hasmcs 124-130 v-rel reticuloendotheliosis viral oncogene homolog A (avian) Mus musculus 105-108 25386047-6 2014 The HO-1 inducer CoPPIX decreased IL-1beta-induced cell proliferation, whereas the HO-1 enzyme inhibitor ZnPPIX significantly reversed EGCG-caused growth inhibition in IL-1beta-treated HASMCs. hasmcs 185-191 interleukin 1 beta Homo sapiens 168-176 24349900-7 2013 Expression of HIF- 1alpha protein and the HIF-1-downstream genes were low under 20% O2 conditions and increased in response to PGE1 treatment in both HUVECs and HASMCs in a dose- and time-dependent manner under 20% O2 conditions as comparable to exposure to 1% O2 conditions. hasmcs 161-167 hypoxia inducible factor 1 subunit alpha Homo sapiens 14-25 24441913-7 2014 Moreover, we examined the effects of the suppression of DNMT1 and/or alkaline phosphatase(ALP) on the mineralization of HASMCs. hasmcs 120-126 ATHS Homo sapiens 90-93 24349900-7 2013 Expression of HIF- 1alpha protein and the HIF-1-downstream genes were low under 20% O2 conditions and increased in response to PGE1 treatment in both HUVECs and HASMCs in a dose- and time-dependent manner under 20% O2 conditions as comparable to exposure to 1% O2 conditions. hasmcs 161-167 hypoxia inducible factor 1 subunit alpha Homo sapiens 42-47 23662399-4 2013 CONCLUSION: TLR4 on passively sensitized HASMCs activated can induce the excessive proliferation of HASMCs and a large number of synthesis and secretion of TGF-beta1, resulting in changing airway micro-environment, which involved in airway remodeling in asthma. hasmcs 41-47 toll like receptor 4 Homo sapiens 12-16 23416929-7 2013 RESULTS: AGEs (100 mug/mL) significantly enhanced Pi-induced calcification and the levels of osteocalcin and Cbfalpha1 in HASMCs. hasmcs 122-128 bone gamma-carboxyglutamate protein Homo sapiens 93-104 23416929-7 2013 RESULTS: AGEs (100 mug/mL) significantly enhanced Pi-induced calcification and the levels of osteocalcin and Cbfalpha1 in HASMCs. hasmcs 122-128 RUNX family transcription factor 2 Homo sapiens 109-118 23175425-6 2013 The results of the present study demonstrated that AQEE attenuated intercellular adhesion molecule 1, E-selectin and COX-2 expression in TNF-alpha-stimulated HASMCs and inhibited THP-1 cell adhesion to activated HASMCs. hasmcs 158-164 tumor necrosis factor Homo sapiens 137-146 23662399-4 2013 CONCLUSION: TLR4 on passively sensitized HASMCs activated can induce the excessive proliferation of HASMCs and a large number of synthesis and secretion of TGF-beta1, resulting in changing airway micro-environment, which involved in airway remodeling in asthma. hasmcs 41-47 transforming growth factor beta 1 Homo sapiens 156-165 22884153-9 2012 Antiproliferative effects of telmisartan were observed when HASMCs were treated with the PPAR-gamma antagonist GW9662 but antiproliferative effects of the PPAR-gamma activator pioglitazone were not observed. hasmcs 60-66 peroxisome proliferator activated receptor gamma Homo sapiens 89-99 22842919-6 2012 Ang II caused the contraction of HASMCs; this effect was reversed by Ang-(1-7). hasmcs 33-39 angiotensinogen Homo sapiens 0-6 22842919-8 2012 Furthermore, Y-27632, an inhibitor of ROCK2, attenuated the Ang II-induced contraction of HASMCs by blocking the RhoA/ROCK2 signaling pathway which is involved in this contraction, and thus may be a major regulator involved in the basal maintenance of contractility in HASMCs. hasmcs 90-96 Rho associated coiled-coil containing protein kinase 2 Homo sapiens 38-43 22842919-8 2012 Furthermore, Y-27632, an inhibitor of ROCK2, attenuated the Ang II-induced contraction of HASMCs by blocking the RhoA/ROCK2 signaling pathway which is involved in this contraction, and thus may be a major regulator involved in the basal maintenance of contractility in HASMCs. hasmcs 90-96 angiotensinogen Homo sapiens 60-66 22842919-8 2012 Furthermore, Y-27632, an inhibitor of ROCK2, attenuated the Ang II-induced contraction of HASMCs by blocking the RhoA/ROCK2 signaling pathway which is involved in this contraction, and thus may be a major regulator involved in the basal maintenance of contractility in HASMCs. hasmcs 90-96 ras homolog family member A Homo sapiens 113-117 22842919-8 2012 Furthermore, Y-27632, an inhibitor of ROCK2, attenuated the Ang II-induced contraction of HASMCs by blocking the RhoA/ROCK2 signaling pathway which is involved in this contraction, and thus may be a major regulator involved in the basal maintenance of contractility in HASMCs. hasmcs 90-96 Rho associated coiled-coil containing protein kinase 2 Homo sapiens 118-123 22021110-2 2012 We previously demonstrated that a glucan-containing extract of Reishi polysaccharides (EORP) has the potent anti-inflammatory action of reducing ICAM-1 expression in lipopolysaccharide (LPS)-treated human aortic smooth muscle cells (HASMCs) and LPS-treated mice. hasmcs 233-239 intercellular adhesion molecule 1 Homo sapiens 145-151 22021110-9 2012 Our data show that EORP interferes with the mitogenic activation of JNK, preventing entry of HASMCs into the cell cycle in vitro and reducing cell proliferation in the neointima and decreasing the neointimal area in vivo. hasmcs 93-99 mitogen-activated protein kinase 8 Mus musculus 68-71 22555846-5 2012 IS promoted protein expression of p53, p21, and senescence-associated beta-galactosidase (SA-beta-gal) activity in HASMCs, and N-acetylcysteine and pifithrin-alpha,p-nitro, a p53 inhibitor, blocked these effects. hasmcs 115-121 Wistar clone pR53P1 p53 pseudogene Rattus norvegicus 34-37 22142686-4 2012 Also, in vitro assays of proliferation, migration and expression of matrix metalloproteinase-2 (MMP-2) in human aortic smooth muscle cells (HASMCs) were carried out using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, transwell boyden chamber method and gelatin zymography, respectively. hasmcs 140-146 matrix metallopeptidase 2 Homo sapiens 68-94 22142686-4 2012 Also, in vitro assays of proliferation, migration and expression of matrix metalloproteinase-2 (MMP-2) in human aortic smooth muscle cells (HASMCs) were carried out using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, transwell boyden chamber method and gelatin zymography, respectively. hasmcs 140-146 matrix metallopeptidase 2 Homo sapiens 96-101 22074662-7 2012 Our data show that PEDF increases PPARgamma activation, preventing entry of HASMCs into the cell cycle in vitro and reducing the neointimal area and cell proliferation in the neointima in vivo. hasmcs 76-82 serpin family F member 1 Homo sapiens 19-23 22085644-5 2012 Here we show that small interfering (si)RNA-mediated knockdown of the desmin gene in HASMCs, recombinant HASMCs (reHASMCs), up-regulates Ankrd1 expression. hasmcs 85-91 desmin Homo sapiens 70-76 22085644-5 2012 Here we show that small interfering (si)RNA-mediated knockdown of the desmin gene in HASMCs, recombinant HASMCs (reHASMCs), up-regulates Ankrd1 expression. hasmcs 85-91 ankyrin repeat domain 1 Homo sapiens 137-143 22085644-5 2012 Here we show that small interfering (si)RNA-mediated knockdown of the desmin gene in HASMCs, recombinant HASMCs (reHASMCs), up-regulates Ankrd1 expression. hasmcs 105-111 desmin Homo sapiens 70-76 22085644-5 2012 Here we show that small interfering (si)RNA-mediated knockdown of the desmin gene in HASMCs, recombinant HASMCs (reHASMCs), up-regulates Ankrd1 expression. hasmcs 105-111 ankyrin repeat domain 1 Homo sapiens 137-143 22085644-6 2012 Moreover, loss of desmin in HASMCs increases the phosphorylation of Akt, inhibitor of kappaB kinase (IKK)-alpha, and inhibitor of kappaB (IkappaB)-alpha proteins, leading to NF-kappaB activation. hasmcs 28-34 desmin Homo sapiens 18-24 22085644-6 2012 Moreover, loss of desmin in HASMCs increases the phosphorylation of Akt, inhibitor of kappaB kinase (IKK)-alpha, and inhibitor of kappaB (IkappaB)-alpha proteins, leading to NF-kappaB activation. hasmcs 28-34 AKT serine/threonine kinase 1 Homo sapiens 68-71 22085644-6 2012 Moreover, loss of desmin in HASMCs increases the phosphorylation of Akt, inhibitor of kappaB kinase (IKK)-alpha, and inhibitor of kappaB (IkappaB)-alpha proteins, leading to NF-kappaB activation. hasmcs 28-34 NFKB inhibitor alpha Homo sapiens 117-152 22085644-6 2012 Moreover, loss of desmin in HASMCs increases the phosphorylation of Akt, inhibitor of kappaB kinase (IKK)-alpha, and inhibitor of kappaB (IkappaB)-alpha proteins, leading to NF-kappaB activation. hasmcs 28-34 nuclear factor kappa B subunit 1 Homo sapiens 174-183 23082189-3 2012 METHODS AND RESULTS: RT-PCR revealed that elevated stretch (16% elongation, 1 Hz) increased miR-21 expression in cultured HASMCs, and moderate stretch (10% elongation, 1 Hz) decreased the expression. hasmcs 122-128 microRNA 21 Homo sapiens 92-98 23038096-5 2012 Azelnidipine, a dihydropyridine subclass of CCBs, significantly decreased alkaline phosphatase (ALP) activity of Msx2-overexpressed HASMCs, whereas verapamil and diltiazem had no effect. hasmcs 132-138 alkaline phosphatase, placental Homo sapiens 74-94 23038096-5 2012 Azelnidipine, a dihydropyridine subclass of CCBs, significantly decreased alkaline phosphatase (ALP) activity of Msx2-overexpressed HASMCs, whereas verapamil and diltiazem had no effect. hasmcs 132-138 alkaline phosphatase, placental Homo sapiens 96-99 23038096-5 2012 Azelnidipine, a dihydropyridine subclass of CCBs, significantly decreased alkaline phosphatase (ALP) activity of Msx2-overexpressed HASMCs, whereas verapamil and diltiazem had no effect. hasmcs 132-138 msh homeobox 2 Homo sapiens 113-117 23038096-6 2012 Furthermore, azelnidipine, but not verapamil and diltiazem, significantly decreased matrix mineralization of Msx2-overexpressing HASMCs. hasmcs 129-135 msh homeobox 2 Homo sapiens 109-113 22060290-4 2011 METHODS: Zymography, reverse transcription polymerase chain reaction and immunoblot analyses were used for analysis of MMP expression of TNF-alpha-stimulated HASMCs. hasmcs 158-164 tumor necrosis factor Homo sapiens 137-146 22060290-7 2011 KEY FINDINGS: The supplement of HASMCs with Cholestin extract significantly suppresses enzymatic activities of MMP-2 and MMP-9 in TNF-alpha-stimulated HASMCs. hasmcs 32-38 matrix metallopeptidase 2 Homo sapiens 111-116 22060290-7 2011 KEY FINDINGS: The supplement of HASMCs with Cholestin extract significantly suppresses enzymatic activities of MMP-2 and MMP-9 in TNF-alpha-stimulated HASMCs. hasmcs 32-38 matrix metallopeptidase 9 Homo sapiens 121-126 22060290-7 2011 KEY FINDINGS: The supplement of HASMCs with Cholestin extract significantly suppresses enzymatic activities of MMP-2 and MMP-9 in TNF-alpha-stimulated HASMCs. hasmcs 32-38 tumor necrosis factor Homo sapiens 130-139 22060290-7 2011 KEY FINDINGS: The supplement of HASMCs with Cholestin extract significantly suppresses enzymatic activities of MMP-2 and MMP-9 in TNF-alpha-stimulated HASMCs. hasmcs 151-157 matrix metallopeptidase 2 Homo sapiens 111-116 22060290-7 2011 KEY FINDINGS: The supplement of HASMCs with Cholestin extract significantly suppresses enzymatic activities of MMP-2 and MMP-9 in TNF-alpha-stimulated HASMCs. hasmcs 151-157 matrix metallopeptidase 9 Homo sapiens 121-126 22060290-7 2011 KEY FINDINGS: The supplement of HASMCs with Cholestin extract significantly suppresses enzymatic activities of MMP-2 and MMP-9 in TNF-alpha-stimulated HASMCs. hasmcs 151-157 tumor necrosis factor Homo sapiens 130-139 22060290-10 2011 Furthermore, Cholestin also attenuates intracellular ROS generation in TNF-alpha-treated HASMCs. hasmcs 89-95 tumor necrosis factor Homo sapiens 71-80 21659522-6 2011 Transient transfection of HASMCs with human CSE (hCSE) promoter/luciferase reporter revealed that the region between -226 to +140 base pair contains the core promoter for the hCSE gene. hasmcs 26-32 cystathionine gamma-lyase Homo sapiens 44-47 21659522-6 2011 Transient transfection of HASMCs with human CSE (hCSE) promoter/luciferase reporter revealed that the region between -226 to +140 base pair contains the core promoter for the hCSE gene. hasmcs 26-32 choreoathetosis/spasticity, episodic (paroxysmal choreoathetosis/spasticity) Homo sapiens 49-53 21659522-6 2011 Transient transfection of HASMCs with human CSE (hCSE) promoter/luciferase reporter revealed that the region between -226 to +140 base pair contains the core promoter for the hCSE gene. hasmcs 26-32 choreoathetosis/spasticity, episodic (paroxysmal choreoathetosis/spasticity) Homo sapiens 175-179 21659522-8 2011 Incubation of HASMCs with Sp1 binding inhibitor mithramycin inhibited CSE mRNA expression in a dose-dependent manner. hasmcs 14-20 cystathionine gamma-lyase Homo sapiens 70-73 21276846-8 2011 From these results, it was found that the gelatinolytic activity was regulated (1) by enzymatic inhibition of both MMP-9 and MMP-2, as well as (2) by the decreased production of MMP-9 via ERK pathways in EFA treated HASMCs. hasmcs 216-222 matrix metallopeptidase 9 Homo sapiens 115-120 21690049-5 2011 CONCLUSION: IL-22R1 in HASMCs might be involved in the pathogenesis of asthma, and the therapeutic effect of dexamethasone on asthma is mediated, at least partially, by IL-22R1. hasmcs 23-29 interleukin 22 receptor subunit alpha 1 Homo sapiens 12-19 21690049-6 2011 The effects of IFN-gamma, IL-4, and TGF-beta on asthma may also be attributed to their actions on HASMCs. hasmcs 98-104 interferon gamma Homo sapiens 15-24 21690049-6 2011 The effects of IFN-gamma, IL-4, and TGF-beta on asthma may also be attributed to their actions on HASMCs. hasmcs 98-104 interleukin 4 Homo sapiens 26-30 21690049-6 2011 The effects of IFN-gamma, IL-4, and TGF-beta on asthma may also be attributed to their actions on HASMCs. hasmcs 98-104 transforming growth factor beta 1 Homo sapiens 36-44 21276846-8 2011 From these results, it was found that the gelatinolytic activity was regulated (1) by enzymatic inhibition of both MMP-9 and MMP-2, as well as (2) by the decreased production of MMP-9 via ERK pathways in EFA treated HASMCs. hasmcs 216-222 matrix metallopeptidase 9 Homo sapiens 178-183 21276846-8 2011 From these results, it was found that the gelatinolytic activity was regulated (1) by enzymatic inhibition of both MMP-9 and MMP-2, as well as (2) by the decreased production of MMP-9 via ERK pathways in EFA treated HASMCs. hasmcs 216-222 mitogen-activated protein kinase 1 Homo sapiens 188-191 21781516-11 2011 (4) 1,25-(OH)(2)D(3) significantly inhibited the MMP-9 and ADAM33 mRNA levels in passively sensitized HASMCs by (52.2 +- 2.5)% and (67.8 +- 3.2)%, respectively (P < 0.01). hasmcs 102-108 matrix metallopeptidase 9 Homo sapiens 49-54 21781516-11 2011 (4) 1,25-(OH)(2)D(3) significantly inhibited the MMP-9 and ADAM33 mRNA levels in passively sensitized HASMCs by (52.2 +- 2.5)% and (67.8 +- 3.2)%, respectively (P < 0.01). hasmcs 102-108 ADAM metallopeptidase domain 33 Homo sapiens 59-65 21266537-6 2011 Either mRNA or protein level of DNA methyltransferase 3b (DNMT3b) showed a dose-dependent down-regulation in oxLDL-mediated HASMCs. hasmcs 124-130 DNA methyltransferase 3 beta Homo sapiens 32-56 21471203-5 2011 Notably, overexpression of the Notch1 intracellular domain (N1-ICD) markedly enhanced BMP2-mediated induction of ALP activity and mineralization of HASMCs. hasmcs 148-154 notch receptor 1 Homo sapiens 31-37 21471203-5 2011 Notably, overexpression of the Notch1 intracellular domain (N1-ICD) markedly enhanced BMP2-mediated induction of ALP activity and mineralization of HASMCs. hasmcs 148-154 bone morphogenetic protein 2 Homo sapiens 86-90 21266537-6 2011 Either mRNA or protein level of DNA methyltransferase 3b (DNMT3b) showed a dose-dependent down-regulation in oxLDL-mediated HASMCs. hasmcs 124-130 DNA methyltransferase 3 beta Homo sapiens 58-64 21512281-12 2011 The ALP activity induced in RAGE-overexpressing HASMCs by human serum was positively correlated with the serum creatinine level, but not with phosphate and hemoglobin A1c levels. hasmcs 48-54 alkaline phosphatase, placental Homo sapiens 4-7 20725790-11 2011 Transfection of HASMCs with TLR4 siRNA-1 resulted in down-regulation of LPS-induced expression of MMP-9. hasmcs 16-22 toll like receptor 4 Homo sapiens 28-32 20725790-11 2011 Transfection of HASMCs with TLR4 siRNA-1 resulted in down-regulation of LPS-induced expression of MMP-9. hasmcs 16-22 matrix metallopeptidase 9 Homo sapiens 98-103 21897042-5 2011 Further studies revealed that the GSPB2 pretreatment markedly attenuated the degradation of IkappaB-alpha and nuclear translocation of NF-kappaB by modulating ubiquitination of IkappaB-alpha in AGE-exposed HASMCs. hasmcs 206-212 nuclear factor kappa B subunit 1 Homo sapiens 135-144 21897042-5 2011 Further studies revealed that the GSPB2 pretreatment markedly attenuated the degradation of IkappaB-alpha and nuclear translocation of NF-kappaB by modulating ubiquitination of IkappaB-alpha in AGE-exposed HASMCs. hasmcs 206-212 NFKB inhibitor alpha Homo sapiens 177-190 21512281-12 2011 The ALP activity induced in RAGE-overexpressing HASMCs by human serum was positively correlated with the serum creatinine level, but not with phosphate and hemoglobin A1c levels. hasmcs 48-54 advanced glycosylation end-product specific receptor Homo sapiens 28-32 20938207-7 2011 Immunoblotting confirmed HIF-1alpha, Ets-1, VEGF and MMP-2 are up-regulated in HASMC exposed to hypoxia (p < 0.05), while transcription for MMP-1, MT-MMP-1, MMP-9, MMP-2 and MMP-7 (p < 0.05) increased in hypoxic HASMCs. hasmcs 218-224 matrix metallopeptidase 2 Homo sapiens 53-58 21829524-5 2011 Adiponectin significantly increased P4Halpha1 mRNA and protein levels in IL-6-stimulated HASMCs in a dose- and time-dependent manner. hasmcs 89-95 adiponectin, C1Q and collagen domain containing Homo sapiens 0-11 21829524-5 2011 Adiponectin significantly increased P4Halpha1 mRNA and protein levels in IL-6-stimulated HASMCs in a dose- and time-dependent manner. hasmcs 89-95 interleukin 6 Homo sapiens 73-77 21829524-6 2011 As well, ERK1/2 and Sp1 played a crucial role in the effect of adiponectin upregulating P4Halpha1 expression in IL-6-stimulated HASMCs. hasmcs 128-134 mitogen-activated protein kinase 3 Homo sapiens 9-15 21829524-6 2011 As well, ERK1/2 and Sp1 played a crucial role in the effect of adiponectin upregulating P4Halpha1 expression in IL-6-stimulated HASMCs. hasmcs 128-134 adiponectin, C1Q and collagen domain containing Homo sapiens 63-74 21829524-6 2011 As well, ERK1/2 and Sp1 played a crucial role in the effect of adiponectin upregulating P4Halpha1 expression in IL-6-stimulated HASMCs. hasmcs 128-134 interleukin 6 Homo sapiens 112-116 20618305-9 2010 ERK1/2 is activated by HSP and DSP in HASMCs and inhibition of ERK1/2 abrogated the smoke extracts-induced HASMC proliferation, while blockage of nicotinic receptors had no effects, suggesting that the toxic effects of the smoke extracts occur via activation of intracellular ERK1/2 signalling, but not nicotinic receptors. hasmcs 38-44 mitogen activated protein kinase 3 Rattus norvegicus 0-6 21057728-4 2010 Serum-starved HASMCs were treated with DSPs for up to 48 h. DSPs promoted cell proliferation in a concentration-dependent manner from 0.05 to 0.2 mul/ml. hasmcs 14-20 DSPS Homo sapiens 60-64 20930172-2 2010 METHODS AND RESULTS: The addition of nonlipidated tissue factor and Ca(2+) to HASMCs maintained in reptilase-treated platelet-poor plasma resulted in the robust formation of thrombin after a lag phase of approximately 6 minutes. hasmcs 78-84 coagulation factor II, thrombin Homo sapiens 174-182 20930172-6 2010 PAR-3 AP or PAR-4 AP enhanced tissue factor-induced factor Xa production and phosphatidylserine exposure on the surface of HASMCs. hasmcs 123-129 coagulation factor II thrombin receptor like 2 Homo sapiens 0-5 20930172-6 2010 PAR-3 AP or PAR-4 AP enhanced tissue factor-induced factor Xa production and phosphatidylserine exposure on the surface of HASMCs. hasmcs 123-129 nuclear receptor subfamily 1 group I member 2 Homo sapiens 0-3 20801891-6 2010 Cytokine release from HASMCs exposed to tumor necrosis factor alpha (TNFalpha), dexamethasone, and/or resveratrol was measured via enzyme-linked immunosorbent assay and compared between nonsmokers (NS), smokers without COPD (S), and smokers with COPD (all n = 10). hasmcs 22-28 tumor necrosis factor Homo sapiens 40-67 20801891-6 2010 Cytokine release from HASMCs exposed to tumor necrosis factor alpha (TNFalpha), dexamethasone, and/or resveratrol was measured via enzyme-linked immunosorbent assay and compared between nonsmokers (NS), smokers without COPD (S), and smokers with COPD (all n = 10). hasmcs 22-28 tumor necrosis factor Homo sapiens 69-77 21057728-9 2010 DSPs increased the expression of intercellular adhesion molecule 1 and the release of interleukin-6 in HASMCs, both of which were inhibited by ERK1/2 or NF-kappaB pathway inhibitors. hasmcs 103-109 DSPS Homo sapiens 0-4 21057728-9 2010 DSPs increased the expression of intercellular adhesion molecule 1 and the release of interleukin-6 in HASMCs, both of which were inhibited by ERK1/2 or NF-kappaB pathway inhibitors. hasmcs 103-109 interleukin 6 Homo sapiens 86-99 21057728-9 2010 DSPs increased the expression of intercellular adhesion molecule 1 and the release of interleukin-6 in HASMCs, both of which were inhibited by ERK1/2 or NF-kappaB pathway inhibitors. hasmcs 103-109 mitogen-activated protein kinase 3 Homo sapiens 143-149 21057728-9 2010 DSPs increased the expression of intercellular adhesion molecule 1 and the release of interleukin-6 in HASMCs, both of which were inhibited by ERK1/2 or NF-kappaB pathway inhibitors. hasmcs 103-109 nuclear factor kappa B subunit 1 Homo sapiens 153-162 20697944-1 2010 OBJECTIVE: To investigate the relationship between the proliferation of sensitized human airway smooth muscle cells (HASMCs) and the expression of extracellular signal regulated kinase (ERK) and the effect of Shenmai Injection (SMI) on HASMCs. hasmcs 117-123 mitogen-activated protein kinase 1 Homo sapiens 147-184 20687608-4 2010 Immunoprecipitation and real-time polymerase chain reaction demonstrated that EORP markedly reduced the interaction of human antigen R protein (HuR) with the 3"-UTR of ICAM-1 mRNA in LPS-stimulated HASMCs. hasmcs 198-204 ELAV (embryonic lethal, abnormal vision)-like 1 (Hu antigen R) Mus musculus 119-142 20687608-4 2010 Immunoprecipitation and real-time polymerase chain reaction demonstrated that EORP markedly reduced the interaction of human antigen R protein (HuR) with the 3"-UTR of ICAM-1 mRNA in LPS-stimulated HASMCs. hasmcs 198-204 ELAV (embryonic lethal, abnormal vision)-like 1 (Hu antigen R) Mus musculus 144-147 20687608-4 2010 Immunoprecipitation and real-time polymerase chain reaction demonstrated that EORP markedly reduced the interaction of human antigen R protein (HuR) with the 3"-UTR of ICAM-1 mRNA in LPS-stimulated HASMCs. hasmcs 198-204 intercellular adhesion molecule 1 Mus musculus 168-174 20511342-3 2010 Here, we show that mechanical stretch of HASMCs augmented TGF-beta1 expression through a de novo RNA synthesis mechanism. hasmcs 41-47 transforming growth factor beta 1 Homo sapiens 58-67 20511342-9 2010 Either treatment of HASMCs with the inhibitors of RhoA, ROCK1/2, PTK, PI3K, MEK1/2, or AP-1 or transfection of HASMCs with AP-1 decoy oligonucleotide attenuated stretch-induced TGF-beta1 expression through repressing the DNA binding activity of AP-1. hasmcs 20-26 protein tyrosine kinase 2 beta Homo sapiens 65-68 20697944-1 2010 OBJECTIVE: To investigate the relationship between the proliferation of sensitized human airway smooth muscle cells (HASMCs) and the expression of extracellular signal regulated kinase (ERK) and the effect of Shenmai Injection (SMI) on HASMCs. hasmcs 117-123 mitogen-activated protein kinase 1 Homo sapiens 186-189 20697944-6 2010 At the same time, the expression of p-ERK1/2 in passively sensitized HASMCs was significantly increased compared with the control group (all P<0.05). hasmcs 69-75 mitogen-activated protein kinase 3 Homo sapiens 38-44 20697944-11 2010 The expression of ERK can be inhibited by SMI in a dose-dependent manner, thus preventing the proliferation of HASMCs. hasmcs 111-117 mitogen-activated protein kinase 1 Homo sapiens 18-21 20979800-5 2010 Then, HASMCs were transfected with ERK sense, antisense and mismatched oligodeoxynucleotides (ODN). hasmcs 6-12 mitogen-activated protein kinase 1 Homo sapiens 35-38 20670427-6 2010 METHOD: Expression of oxytocin receptor in cultured HASMCs was performed by real time PCR and flow cytomery assays. hasmcs 52-58 oxytocin receptor Homo sapiens 22-39 20670427-10 2010 Additionally, oxytocin increases cytosolic calcium levels in fura-2-loaded HASMCs that were enhanced in cells treated for 24 hr with IL-13. hasmcs 75-81 interleukin 13 Homo sapiens 133-138 20670427-14 2010 CONCLUSION: Taken together, we show that cytokines modulate the expression of functional oxytocin receptors in HASMCs suggesting a potential role for inflammation-induced changes in oxytocin receptor signaling in the regulation of airway hyper-responsiveness in asthma. hasmcs 111-117 oxytocin receptor Homo sapiens 89-106 20979800-12 2010 The expression of ERK mRNA (0.43 +- 0.06) and the activation ratio of ERK (63 +- 6)% in HASMCs from group AODNs were significantly decreased compared to those of chronic asthma group, which were (0.89 +- 0.09), (87 +- 8)%, respectively (F = 78.043, 87.288, respectively, P < 0.05). hasmcs 88-94 mitogen-activated protein kinase 1 Homo sapiens 18-21 20979800-12 2010 The expression of ERK mRNA (0.43 +- 0.06) and the activation ratio of ERK (63 +- 6)% in HASMCs from group AODNs were significantly decreased compared to those of chronic asthma group, which were (0.89 +- 0.09), (87 +- 8)%, respectively (F = 78.043, 87.288, respectively, P < 0.05). hasmcs 88-94 mitogen-activated protein kinase 1 Homo sapiens 70-73 20979800-13 2010 ERK antisense ODN inhibited the proliferation of HASMCs and induced the apoptosis of HASMCs, but the sense and the mismatched ones did not have these effects. hasmcs 49-55 mitogen-activated protein kinase 1 Homo sapiens 0-3 20979800-13 2010 ERK antisense ODN inhibited the proliferation of HASMCs and induced the apoptosis of HASMCs, but the sense and the mismatched ones did not have these effects. hasmcs 85-91 mitogen-activated protein kinase 1 Homo sapiens 0-3 20979800-14 2010 CONCLUSIONS: ERK antisense ODN inhibited the proliferation and increased the apoptosis in cultured HASMCs passively sensitized with 10% serum from asthmatic patients. hasmcs 99-105 mitogen-activated protein kinase 1 Homo sapiens 13-16 20979800-15 2010 The result suggests that ERK signaling pathway may contribute to the proliferation and apoptosis of HASMCs in asthmatic patients. hasmcs 100-106 mitogen-activated protein kinase 1 Homo sapiens 25-28 19447587-4 2010 METHODS AND RESULTS: The Matrigel migration assay showed that curcumin (10 and 20 micromol/l) effectively inhibited TNF-alpha-induced migration of HASMCs as compared with the control group. hasmcs 147-153 tumor necrosis factor Homo sapiens 116-125 20426528-10 2010 The activation of PKC alpha with phorbol myristate acetate (PMA), a PKC activator, up-regulated cyclin D1 expression and increased the proliferation of passively sensitized HASMCs. hasmcs 173-179 protein kinase C alpha Homo sapiens 18-27 20426528-10 2010 The activation of PKC alpha with phorbol myristate acetate (PMA), a PKC activator, up-regulated cyclin D1 expression and increased the proliferation of passively sensitized HASMCs. hasmcs 173-179 protein kinase C alpha Homo sapiens 18-21 20426528-13 2010 These results demonstrate that PKC alpha promotes the proliferation of HASMCs sensitized with atopic asthmatic serum via up-regulation of cyclin D1 expression. hasmcs 71-77 protein kinase C alpha Homo sapiens 31-40 20426528-13 2010 These results demonstrate that PKC alpha promotes the proliferation of HASMCs sensitized with atopic asthmatic serum via up-regulation of cyclin D1 expression. hasmcs 71-77 cyclin D1 Homo sapiens 138-147 20044439-10 2010 The present study profiles the regulatory relationship between LPA and multiple cytokines in vascular SMCs for the first time, provides the first evidence that LPA upregulates IL-6 in vascular SMCs, and reveals the regulatory mechanism of LPA-induced IL-6 production in HASMCs. hasmcs 270-276 interleukin 6 Homo sapiens 251-255 20067268-5 2010 The results show that genistein significantly reduced cell proliferation in TNF-alpha-induced HASMCs. hasmcs 94-100 tumor necrosis factor Homo sapiens 76-85 20067268-7 2010 In addition, genistein increased nucleosomal DNA fragmentation, increased the expression levels of Bax and c-Myc, and decreased the expression levels of Bcl-2 and Bcl-xL in TNF-alpha-induced HASMCs. hasmcs 191-197 MYC proto-oncogene, bHLH transcription factor Homo sapiens 107-112 20067268-7 2010 In addition, genistein increased nucleosomal DNA fragmentation, increased the expression levels of Bax and c-Myc, and decreased the expression levels of Bcl-2 and Bcl-xL in TNF-alpha-induced HASMCs. hasmcs 191-197 BCL2 apoptosis regulator Homo sapiens 153-158 20067268-7 2010 In addition, genistein increased nucleosomal DNA fragmentation, increased the expression levels of Bax and c-Myc, and decreased the expression levels of Bcl-2 and Bcl-xL in TNF-alpha-induced HASMCs. hasmcs 191-197 BCL2 like 1 Homo sapiens 163-169 20067268-7 2010 In addition, genistein increased nucleosomal DNA fragmentation, increased the expression levels of Bax and c-Myc, and decreased the expression levels of Bcl-2 and Bcl-xL in TNF-alpha-induced HASMCs. hasmcs 191-197 tumor necrosis factor Homo sapiens 173-182 20067268-8 2010 Taken together, these findings indicate that genistein regulates the activation of apoptosis-related molecules in TNF-alpha-induced HASMCs, leading to the suppression of proliferation and induction of apoptosis. hasmcs 132-138 tumor necrosis factor Homo sapiens 114-123 19447587-8 2010 CONCLUSION: These results indicate that curcumin has anti-inflammatory properties and may prevent the migration of HASMCs by suppressing MMP-9 expression through down-regulation of NF-kappaB. hasmcs 115-121 matrix metallopeptidase 9 Homo sapiens 137-142 19666008-7 2009 Taken together, we demonstrated for the first time that Ang II activates ERK5 via the AT1/PKC/PKD pathway and revealed a critical role of ERK5 in Ang II-induced HASMCs hypertrophy. hasmcs 161-167 angiogenin Homo sapiens 56-59 19666008-7 2009 Taken together, we demonstrated for the first time that Ang II activates ERK5 via the AT1/PKC/PKD pathway and revealed a critical role of ERK5 in Ang II-induced HASMCs hypertrophy. hasmcs 161-167 angiotensin II receptor type 1 Homo sapiens 86-89 19666008-7 2009 Taken together, we demonstrated for the first time that Ang II activates ERK5 via the AT1/PKC/PKD pathway and revealed a critical role of ERK5 in Ang II-induced HASMCs hypertrophy. hasmcs 161-167 protein kinase D1 Homo sapiens 94-97 19666008-7 2009 Taken together, we demonstrated for the first time that Ang II activates ERK5 via the AT1/PKC/PKD pathway and revealed a critical role of ERK5 in Ang II-induced HASMCs hypertrophy. hasmcs 161-167 mitogen-activated protein kinase 7 Homo sapiens 138-142 19666008-7 2009 Taken together, we demonstrated for the first time that Ang II activates ERK5 via the AT1/PKC/PKD pathway and revealed a critical role of ERK5 in Ang II-induced HASMCs hypertrophy. hasmcs 161-167 angiogenin Homo sapiens 146-149 19726333-7 2009 RT-PCR and Western blotting showed that infection with the recombinant adenovirus resulted in PTEN overexpression in the HASMCs, causing also increased ratio of G(0)/G(1) cells and proliferation inhibition of the ASMCs. hasmcs 121-127 phosphatase and tensin homolog Homo sapiens 94-98 19407244-5 2009 A knockdown experiment with a small interfering RNA confirmed that Msx2 mediated N1-ICD-induced osteogenic conversion of HASMCs. hasmcs 121-127 msh homeobox 2 Homo sapiens 67-71 19164326-11 2009 OAT3 was expressed in HASMCs. hasmcs 22-28 solute carrier family 22 member 8 Homo sapiens 0-4 19726333-10 2009 PTEN overexpression can efficiently inhibit the proliferation of HASMCs possibly through the PI3K/PKB/AKt and P21 pathways. hasmcs 65-71 phosphatase and tensin homolog Homo sapiens 0-4 19726333-10 2009 PTEN overexpression can efficiently inhibit the proliferation of HASMCs possibly through the PI3K/PKB/AKt and P21 pathways. hasmcs 65-71 AKT serine/threonine kinase 1 Homo sapiens 102-105 19726333-10 2009 PTEN overexpression can efficiently inhibit the proliferation of HASMCs possibly through the PI3K/PKB/AKt and P21 pathways. hasmcs 65-71 H3 histone pseudogene 16 Homo sapiens 110-113 18978189-4 2009 In contrast to native aorta, cultured hASMCs strongly expressed SCN9A encoding Na(V)1.7, as determined by quantitative RT-PCR. hasmcs 38-44 sodium voltage-gated channel alpha subunit 9 Homo sapiens 64-69 19011954-7 2009 Transfecting HASMCs with small interfering RNA (siRNA) to silence ERK5 inhibited Ang II-induced cell hypertrophy. hasmcs 13-19 mitogen-activated protein kinase 7 Homo sapiens 66-70 19011954-7 2009 Transfecting HASMCs with small interfering RNA (siRNA) to silence ERK5 inhibited Ang II-induced cell hypertrophy. hasmcs 13-19 angiotensinogen Homo sapiens 81-87 17872375-9 2007 In contrast, in HASMCs, E(2), and raloxifene attenuated the serum-induced recruitment of coactivator complexes and histone acetylation at both the IGF-I and COX-2 gene promoters. hasmcs 16-22 insulin like growth factor 1 Homo sapiens 147-152 19142017-5 2009 RESULTS: Inhibitors of caspases 3, 8 and 9 were efficiently blocked by rhein-induced apoptosis in TNF-alpha-treated HASMCs. hasmcs 116-122 caspase 3 Homo sapiens 23-42 19142017-5 2009 RESULTS: Inhibitors of caspases 3, 8 and 9 were efficiently blocked by rhein-induced apoptosis in TNF-alpha-treated HASMCs. hasmcs 116-122 tumor necrosis factor Homo sapiens 98-107 18679784-6 2008 Pulsatile atmospheric pressure increased the amount of phosphorylated extracellular signal-regulated kinase (ERK) by approximately 54% in HASMCs. hasmcs 138-144 mitogen-activated protein kinase 1 Homo sapiens 70-107 18679784-6 2008 Pulsatile atmospheric pressure increased the amount of phosphorylated extracellular signal-regulated kinase (ERK) by approximately 54% in HASMCs. hasmcs 138-144 mitogen-activated protein kinase 1 Homo sapiens 109-112 17979138-6 2008 Moreover, tanshinone IIA inhibited the migration of TNF-alpha-induced HASMCs. hasmcs 70-76 tumor necrosis factor Homo sapiens 52-61 17635014-6 2008 Direct incubation of biomaterial-pretreated monocytes or neutrophils with "more secretory" HASMCs further increased HASMC ICAM-1 and tissue factor expression. hasmcs 91-97 intercellular adhesion molecule 1 Homo sapiens 122-128 17635014-6 2008 Direct incubation of biomaterial-pretreated monocytes or neutrophils with "more secretory" HASMCs further increased HASMC ICAM-1 and tissue factor expression. hasmcs 91-97 coagulation factor III, tissue factor Homo sapiens 133-146 17635014-7 2008 Direct incubation of biomaterial-pretreated monocytes or neutrophils with forced contractile HASMCs upregulated ICAM-1, VCAM-1, and tissue factor expression above the presence of serum-containing media alone. hasmcs 93-99 intercellular adhesion molecule 1 Homo sapiens 112-118 17635014-7 2008 Direct incubation of biomaterial-pretreated monocytes or neutrophils with forced contractile HASMCs upregulated ICAM-1, VCAM-1, and tissue factor expression above the presence of serum-containing media alone. hasmcs 93-99 vascular cell adhesion molecule 1 Homo sapiens 120-126 17635014-7 2008 Direct incubation of biomaterial-pretreated monocytes or neutrophils with forced contractile HASMCs upregulated ICAM-1, VCAM-1, and tissue factor expression above the presence of serum-containing media alone. hasmcs 93-99 coagulation factor III, tissue factor Homo sapiens 132-145 17872375-9 2007 In contrast, in HASMCs, E(2), and raloxifene attenuated the serum-induced recruitment of coactivator complexes and histone acetylation at both the IGF-I and COX-2 gene promoters. hasmcs 16-22 mitochondrially encoded cytochrome c oxidase II Homo sapiens 157-162 17145119-2 2007 In this study, we examined the effects of doxycycline and minocycline on vascular endothelial growth factor (VEGF)-induced human aortic smooth muscle cell (HASMCs) migration, and explored the mechanisms in which doxycycline or minocycline inhibit HASMC migration. hasmcs 156-162 vascular endothelial growth factor A Homo sapiens 73-107 17206382-4 2007 HASMCs expressed measurable levels/activities of GSH, GR, GPx, and GST. hasmcs 0-6 glutathione-disulfide reductase Homo sapiens 54-56 17206382-4 2007 HASMCs expressed measurable levels/activities of GSH, GR, GPx, and GST. hasmcs 0-6 glutathione S-transferase kappa 1 Homo sapiens 67-70 17206382-5 2007 Incubation of HASMCs with low micromolar concentrations of D3T resulted in a marked elevation in total cellular GSH content and GR activity. hasmcs 14-20 glutathione-disulfide reductase Homo sapiens 128-130 17206382-12 2007 Taken together, this study demonstrates that both GSH/GCL and GR in normal HASMCs are inducible by D3T, and that upregulation of GSH biosynthesis appears to be the predominant mechanism underlying D3T-mediated cytoprotection against ROS/RNS-elicited injury to human vascular smooth muscle cells. hasmcs 75-81 glutamate-cysteine ligase catalytic subunit Homo sapiens 54-57 17206382-12 2007 Taken together, this study demonstrates that both GSH/GCL and GR in normal HASMCs are inducible by D3T, and that upregulation of GSH biosynthesis appears to be the predominant mechanism underlying D3T-mediated cytoprotection against ROS/RNS-elicited injury to human vascular smooth muscle cells. hasmcs 75-81 glutathione-disulfide reductase Homo sapiens 62-64 17603281-8 2007 Our findings suggest that curcumin has an ability to induce HO-1 expression, presumably through Nrf2-dependent ARE activation, in rat VSMCs and HASMCs, and provide evidence that the antiproliferative effect of curcumin is considerably linked to its ability to induce HO-1 expression. hasmcs 144-150 heme oxygenase 1 Rattus norvegicus 60-64 17325258-4 2007 Compared with normoglycemia, exposure of HASMCs to hyperglycemia but not mannitol significantly increased sphingosine kinase 1 (SK1) activity but not SK2 activity. hasmcs 41-47 sphingosine kinase 1 Homo sapiens 106-126 17325258-4 2007 Compared with normoglycemia, exposure of HASMCs to hyperglycemia but not mannitol significantly increased sphingosine kinase 1 (SK1) activity but not SK2 activity. hasmcs 41-47 sphingosine kinase 1 Homo sapiens 128-131 17325258-10 2007 We conclude that hyperglycemia stimulates SK1 activity via PKC- and oxidative stress-dependent pathways, leading to decreased apoptosis in HASMCs. hasmcs 139-145 sphingosine kinase 1 Homo sapiens 42-45 17145119-2 2007 In this study, we examined the effects of doxycycline and minocycline on vascular endothelial growth factor (VEGF)-induced human aortic smooth muscle cell (HASMCs) migration, and explored the mechanisms in which doxycycline or minocycline inhibit HASMC migration. hasmcs 156-162 vascular endothelial growth factor A Homo sapiens 109-113 17901563-11 2007 The spin-trapping experiments also showed that induction of NQO1 by D3T in HASMCs augmented the O2(.-) scavenging ability. hasmcs 75-81 NAD(P)H quinone dehydrogenase 1 Rattus norvegicus 60-64 16990552-5 2006 METHODS AND RESULTS: Stimulation of HASMCs with LPS significantly increased the cytosolic HuR level in vitro. hasmcs 36-42 ELAV like RNA binding protein 1 Homo sapiens 90-93 16990552-11 2006 CONCLUSIONS: Activation of NADPH oxidase and the MAPK-signaling pathway contribute to HuR-mediated stabilization of TLR4 mRNA induced by LPS in HASMCs. hasmcs 144-150 ELAV like RNA binding protein 1 Homo sapiens 86-89 16990552-11 2006 CONCLUSIONS: Activation of NADPH oxidase and the MAPK-signaling pathway contribute to HuR-mediated stabilization of TLR4 mRNA induced by LPS in HASMCs. hasmcs 144-150 toll like receptor 4 Homo sapiens 116-120 16518841-8 2006 The inactivation of ERK and PI3K/PKB pathways and modulation of cell-cycle proteins by ginsenoside Rg1 may be of importance in inhibition of HASMCs proliferation. hasmcs 141-147 protein phosphatase 1 regulatory subunit 3A Homo sapiens 99-102 16840713-14 2006 CONCLUSIONS: HO-1 and Akt exert codependent cytoprotective effects against OS-induced apoptosis in HASMCs. hasmcs 99-105 heme oxygenase 1 Homo sapiens 13-17 16840713-14 2006 CONCLUSIONS: HO-1 and Akt exert codependent cytoprotective effects against OS-induced apoptosis in HASMCs. hasmcs 99-105 AKT serine/threonine kinase 1 Homo sapiens 22-25 16574942-6 2006 TNF-alpha treatment also increased both the peak and plateau intracellular Ca(2+) concentration responses in HASMCs elicited by acetylcholine and bradykinin. hasmcs 109-115 tumor necrosis factor Homo sapiens 0-9 16574942-6 2006 TNF-alpha treatment also increased both the peak and plateau intracellular Ca(2+) concentration responses in HASMCs elicited by acetylcholine and bradykinin. hasmcs 109-115 kininogen 1 Homo sapiens 146-156 16809572-7 2006 Proinflammatory cytokines (interleukin-1beta and interferon-gamma) upregulate Fn14 expression in hASMCs. hasmcs 97-103 interleukin 1 beta Homo sapiens 27-44 16809572-7 2006 Proinflammatory cytokines (interleukin-1beta and interferon-gamma) upregulate Fn14 expression in hASMCs. hasmcs 97-103 interferon gamma Homo sapiens 49-65 16809572-7 2006 Proinflammatory cytokines (interleukin-1beta and interferon-gamma) upregulate Fn14 expression in hASMCs. hasmcs 97-103 TNF receptor superfamily member 12A Homo sapiens 78-82 16713603-5 2006 We found that SalB, as well as NADPH oxidase inhibitors, DPI or apocynin, and antioxidant NAC, inhibited TNF-alpha-induced MMP-2 mRNA, protein expression, and gelatinolytic activity in HASMCs in a concentration-dependent manner. hasmcs 185-191 tumor necrosis factor Homo sapiens 105-114 16713603-7 2006 SalB also significantly inhibited angiotensin II or H2O2-induced MMP-2 mRNA and protein expression and gelatinolytic activity in HASMCs. hasmcs 129-135 angiotensinogen Homo sapiens 34-48 16440326-5 2006 In contrast, HASMCs with or without LPS treatment showed constitutive expression of COX-1 mRNA and protein. hasmcs 13-19 cytochrome c oxidase I, mitochondrial Mus musculus 84-89 16440326-6 2006 The activation of COX-2 protein synthesis in LPS-stimulated HASMCs was shown to involve the activation of the extracellular-signal-regulated kinase 1/2 (ERK1/2), c-Jun NH(2)-terminal kinase (JNK), and p38 mitogen-activated protein kinase pathway. hasmcs 60-66 cytochrome c oxidase II, mitochondrial Mus musculus 18-23 16440326-6 2006 The activation of COX-2 protein synthesis in LPS-stimulated HASMCs was shown to involve the activation of the extracellular-signal-regulated kinase 1/2 (ERK1/2), c-Jun NH(2)-terminal kinase (JNK), and p38 mitogen-activated protein kinase pathway. hasmcs 60-66 mitogen-activated protein kinase 3 Mus musculus 110-151 16440326-6 2006 The activation of COX-2 protein synthesis in LPS-stimulated HASMCs was shown to involve the activation of the extracellular-signal-regulated kinase 1/2 (ERK1/2), c-Jun NH(2)-terminal kinase (JNK), and p38 mitogen-activated protein kinase pathway. hasmcs 60-66 mitogen-activated protein kinase 3 Mus musculus 153-159 16440326-6 2006 The activation of COX-2 protein synthesis in LPS-stimulated HASMCs was shown to involve the activation of the extracellular-signal-regulated kinase 1/2 (ERK1/2), c-Jun NH(2)-terminal kinase (JNK), and p38 mitogen-activated protein kinase pathway. hasmcs 60-66 mitogen-activated protein kinase 8 Mus musculus 162-189 16440326-6 2006 The activation of COX-2 protein synthesis in LPS-stimulated HASMCs was shown to involve the activation of the extracellular-signal-regulated kinase 1/2 (ERK1/2), c-Jun NH(2)-terminal kinase (JNK), and p38 mitogen-activated protein kinase pathway. hasmcs 60-66 mitogen-activated protein kinase 8 Mus musculus 191-194 16440326-6 2006 The activation of COX-2 protein synthesis in LPS-stimulated HASMCs was shown to involve the activation of the extracellular-signal-regulated kinase 1/2 (ERK1/2), c-Jun NH(2)-terminal kinase (JNK), and p38 mitogen-activated protein kinase pathway. hasmcs 60-66 mitogen-activated protein kinase 14 Mus musculus 201-204 16440326-7 2006 Incubation of HASMCs with Sal B before LPS stimulation resulted in pronounced downregulation of COX-2 expression. hasmcs 14-20 cytochrome c oxidase II, mitochondrial Mus musculus 96-101 16458614-5 2006 These results suggest that notoginsenoside R1 inhibits TNF-alpha-induced PAI-1 overexpression in HASMCs by suppressing ERK and PKB signaling pathways. hasmcs 97-103 tumor necrosis factor Homo sapiens 55-64 16361361-5 2006 HASMCs were pretreated in some experiments with apyrase, which degrades ATP, and inhibitors of ERK1/2, JNK, and p38 MAPK. hasmcs 0-6 mitogen-activated protein kinase 3 Homo sapiens 95-101 16361361-5 2006 HASMCs were pretreated in some experiments with apyrase, which degrades ATP, and inhibitors of ERK1/2, JNK, and p38 MAPK. hasmcs 0-6 mitogen-activated protein kinase 8 Homo sapiens 103-106 16632126-8 2006 These results suggest that notoginsenoside R1 inhibits TNF-alpha-induced ERK activation and subsequent fibronectin overexpression and migration in HASMCs by suppressing NADPH oxidase-mediated ROS generation and directly scavenging ROS. hasmcs 147-153 tumor necrosis factor Homo sapiens 55-64 16632126-8 2006 These results suggest that notoginsenoside R1 inhibits TNF-alpha-induced ERK activation and subsequent fibronectin overexpression and migration in HASMCs by suppressing NADPH oxidase-mediated ROS generation and directly scavenging ROS. hasmcs 147-153 fibronectin 1 Homo sapiens 103-114 16458614-5 2006 These results suggest that notoginsenoside R1 inhibits TNF-alpha-induced PAI-1 overexpression in HASMCs by suppressing ERK and PKB signaling pathways. hasmcs 97-103 serpin family E member 1 Homo sapiens 73-78 15983226-9 2005 Treatment of HASMCs with metformin decreased leptin-induced ROS production and activation of PKC, ERK1/2, and NF-kappaB. hasmcs 13-19 mitogen-activated protein kinase 3 Homo sapiens 98-104 15983226-9 2005 Treatment of HASMCs with metformin decreased leptin-induced ROS production and activation of PKC, ERK1/2, and NF-kappaB. hasmcs 13-19 nuclear factor kappa B subunit 1 Homo sapiens 110-119 16201268-9 2005 The proliferation of HASMCs of group A2 and B2 stimulated with CSE and group A3 and B3 stimulated with CSE and PMA were also significantly enhanced when group A1, A2 and A3 and group B1, B2 and B3 compared to each other (P<0.05, P<0.01, respectively). hasmcs 21-27 glycoprotein hormone subunit alpha 2 Homo sapiens 163-196 15983226-5 2005 Incubation of HASMCs with leptin enhanced the proliferation and MMP-2 expression in these cells and increased the generation of intracellular reactive oxygen species (ROS). hasmcs 14-20 matrix metallopeptidase 2 Homo sapiens 64-69 15990776-6 2005 METHODS: Cultured HASMCs were stimulated with IL-1beta, TNF-alpha, and IFN-gamma and treated with (R)-enantiomers and (S)-enantiomers of albuterol and formoterol, with and without propranolol and ICI-118,551, and in combination with dexamethasone. hasmcs 18-24 interleukin 1 beta Homo sapiens 46-54 15990776-6 2005 METHODS: Cultured HASMCs were stimulated with IL-1beta, TNF-alpha, and IFN-gamma and treated with (R)-enantiomers and (S)-enantiomers of albuterol and formoterol, with and without propranolol and ICI-118,551, and in combination with dexamethasone. hasmcs 18-24 tumor necrosis factor Homo sapiens 56-65 15990776-6 2005 METHODS: Cultured HASMCs were stimulated with IL-1beta, TNF-alpha, and IFN-gamma and treated with (R)-enantiomers and (S)-enantiomers of albuterol and formoterol, with and without propranolol and ICI-118,551, and in combination with dexamethasone. hasmcs 18-24 interferon gamma Homo sapiens 71-80 15695555-5 2005 Although RT-PCR demonstrated the presence of equilibrative nucleoside transporter-1 (ENT-1) and ENT-2 mRNA, functional studies revealed that adenosine transport in HASMCs was predominantly mediated by ENT-1 and inhibited by nitrobenzylmercaptopurine riboside (NBMPR, IC(50) = 0.69 +/- 0.05 nM). hasmcs 164-170 solute carrier family 29 member 1 (Augustine blood group) Homo sapiens 201-206 15695555-11 2005 We conclude that d-glucose upregulates the protein and message expression and functional activity of ENT-1 in HASMCs, possibly via MAPK/ERK-dependent pathways. hasmcs 110-116 solute carrier family 29 member 1 (Augustine blood group) Homo sapiens 101-106 15695555-11 2005 We conclude that d-glucose upregulates the protein and message expression and functional activity of ENT-1 in HASMCs, possibly via MAPK/ERK-dependent pathways. hasmcs 110-116 mitogen-activated protein kinase 1 Homo sapiens 136-139 14733769-5 2004 The proliferation of HASMCs was examined by cell cycle analysis, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazoliumbromide (MTT) colorimetric assay and proliferating cell nuclear antigen (PCNA) immunofluorescence staining. hasmcs 21-27 proliferating cell nuclear antigen Homo sapiens 154-188 15730731-3 2004 NF-kappaB specific inhibitor pyrrolidine dithiocarbamate (PDTC) and PKC agonist phorbol 12-myristate 13-acetate (PMA) were used to intervene HASMCs exposed to asthmatic serum and non-asthmatic control serum. hasmcs 141-147 nuclear factor kappa B subunit 1 Homo sapiens 0-9 15730731-12 2004 CONCLUSIONS: NF-kappaB may contribute to the proliferation of HASMCs passively sensitized with human asthmatic serum, which involves the PKC/NF-kappaB signal pathway. hasmcs 62-68 nuclear factor kappa B subunit 1 Homo sapiens 13-22 15730731-12 2004 CONCLUSIONS: NF-kappaB may contribute to the proliferation of HASMCs passively sensitized with human asthmatic serum, which involves the PKC/NF-kappaB signal pathway. hasmcs 62-68 nuclear factor kappa B subunit 1 Homo sapiens 141-150 15545519-8 2004 Transient phosphorylation of the p42/44 mitogen-activated protein kinase (ERK 1/2) occurred after addition of resistin to HASMCs. hasmcs 122-128 mitogen-activated protein kinase 3 Homo sapiens 74-81 15545519-9 2004 U0126, a specific inhibitor of ERK phosphorylation, significantly inhibited ERK 1/2 phosphorylation and reduced resistin-simulated proliferation of HASMCs. hasmcs 148-154 mitogen-activated protein kinase 1 Homo sapiens 31-34 15371330-6 2004 Suppression of extracellular signal-regulated kinase (ERK) activity, but not of p38 activity, inhibited the H2S-induced apoptosis of HASMCs. hasmcs 133-139 mitogen-activated protein kinase 1 Homo sapiens 15-52 15371330-6 2004 Suppression of extracellular signal-regulated kinase (ERK) activity, but not of p38 activity, inhibited the H2S-induced apoptosis of HASMCs. hasmcs 133-139 mitogen-activated protein kinase 1 Homo sapiens 54-57 15371330-7 2004 The activation of ERK by H2S in HASMCs was accompanied by increased caspase-3 activity. hasmcs 32-38 mitogen-activated protein kinase 1 Homo sapiens 18-21 15371330-7 2004 The activation of ERK by H2S in HASMCs was accompanied by increased caspase-3 activity. hasmcs 32-38 caspase 3 Homo sapiens 68-77 15371330-11 2004 Of the three investigated MAPKs, only ERK played an active role in mediating H2S-induced apoptosis of HASMCs by activating caspase-3. hasmcs 102-108 mitogen-activated protein kinase 1 Homo sapiens 38-41 15371330-11 2004 Of the three investigated MAPKs, only ERK played an active role in mediating H2S-induced apoptosis of HASMCs by activating caspase-3. hasmcs 102-108 caspase 3 Homo sapiens 123-132 15256478-8 2004 Both ERK1/2 and PI3K/AKt pathways were activated during VEGF-induced HASMCs migration. hasmcs 69-75 mitogen-activated protein kinase 3 Homo sapiens 5-11 15256478-8 2004 Both ERK1/2 and PI3K/AKt pathways were activated during VEGF-induced HASMCs migration. hasmcs 69-75 AKT serine/threonine kinase 1 Homo sapiens 21-24 15256478-8 2004 Both ERK1/2 and PI3K/AKt pathways were activated during VEGF-induced HASMCs migration. hasmcs 69-75 vascular endothelial growth factor A Homo sapiens 56-60 15126566-1 2004 We investigated the mechanisms regulating estrogen receptor (ER) expression in human aortic smooth muscle cells (HASMCs) and the mechanisms by which estradiol inhibits HASMC growth. hasmcs 113-119 estrogen receptor 1 Homo sapiens 61-63 14733769-5 2004 The proliferation of HASMCs was examined by cell cycle analysis, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazoliumbromide (MTT) colorimetric assay and proliferating cell nuclear antigen (PCNA) immunofluorescence staining. hasmcs 21-27 proliferating cell nuclear antigen Homo sapiens 190-194 14733769-6 2004 The effect of PKC agonist phorbol 12-myristate 13-acetate (PMA) and PKC inhibitor Ro-31-8220 on the proliferation of HASMCs exposed to human asthmatic serum and non-asthmatic control serum was also examined by the same methods. hasmcs 117-123 proline rich transmembrane protein 2 Homo sapiens 14-17 14733769-6 2004 The effect of PKC agonist phorbol 12-myristate 13-acetate (PMA) and PKC inhibitor Ro-31-8220 on the proliferation of HASMCs exposed to human asthmatic serum and non-asthmatic control serum was also examined by the same methods. hasmcs 117-123 proline rich transmembrane protein 2 Homo sapiens 68-71 14733769-8 2004 RESULTS: The percentage of S phase, absorbance (value A) and the positive percentage of PCNA protein expression in HASMCs passively sensitized with asthmatic serum were (16.30 +/- 2.68)%, 0.430 +/- 0.060 and (63.4 +/- 7.4)% respectively, which were significantly increased compared with HASMCs treated with control serum [(10.01 +/- 1.38)%, 0.328 +/- 0.034 and (37.2 +/- 4.8)%, respectively] (P < 0.05). hasmcs 115-121 proliferating cell nuclear antigen Homo sapiens 88-92 14733769-9 2004 After HASMCs were passively sensitized with asthmatic serum, they were treated with PMA, the percentage of S phase, value A and the positive percentage of PCNA protein expression were (20.33 +/- 3.39)%, 0.542 +/- 0.065 and (76.0 +/- 8.7)% respectively, which were significantly increased compared with asthmatic serum sensitized HASMCs without PMA(P < 0.05). hasmcs 6-12 proliferating cell nuclear antigen Homo sapiens 155-159 14733769-11 2004 The relative ratio of value A of PKC-alpha mRNA and the positive percentage of PKC-alpha protein expression in passively sensitized HASMCs were 1.23 +/- 0.10 and (61.1 +/- 9.4)% respectively, which were significantly increased compared with HASMCs treated with control serum [1.05 +/- 0.09 and (34.9 +/- 6.7)%, respectively] (P < 0.05). hasmcs 132-138 protein kinase C alpha Homo sapiens 33-42 14733769-11 2004 The relative ratio of value A of PKC-alpha mRNA and the positive percentage of PKC-alpha protein expression in passively sensitized HASMCs were 1.23 +/- 0.10 and (61.1 +/- 9.4)% respectively, which were significantly increased compared with HASMCs treated with control serum [1.05 +/- 0.09 and (34.9 +/- 6.7)%, respectively] (P < 0.05). hasmcs 132-138 protein kinase C alpha Homo sapiens 79-88 12527559-5 2003 Antisense oligodeoxynucleotides specific for p21 attenuate BMP-2-induced inhibition of proliferation when transfected into HASMCs, demonstrating that BMP-2 inhibits PDGF-stimulated proliferation of HASMCs through induction of p21. hasmcs 123-129 cyclin dependent kinase inhibitor 1A Homo sapiens 45-48 12527559-5 2003 Antisense oligodeoxynucleotides specific for p21 attenuate BMP-2-induced inhibition of proliferation when transfected into HASMCs, demonstrating that BMP-2 inhibits PDGF-stimulated proliferation of HASMCs through induction of p21. hasmcs 123-129 bone morphogenetic protein 2 Homo sapiens 59-64 12527559-5 2003 Antisense oligodeoxynucleotides specific for p21 attenuate BMP-2-induced inhibition of proliferation when transfected into HASMCs, demonstrating that BMP-2 inhibits PDGF-stimulated proliferation of HASMCs through induction of p21. hasmcs 123-129 bone morphogenetic protein 2 Homo sapiens 150-155 12527559-5 2003 Antisense oligodeoxynucleotides specific for p21 attenuate BMP-2-induced inhibition of proliferation when transfected into HASMCs, demonstrating that BMP-2 inhibits PDGF-stimulated proliferation of HASMCs through induction of p21. hasmcs 198-204 cyclin dependent kinase inhibitor 1A Homo sapiens 45-48 12527559-5 2003 Antisense oligodeoxynucleotides specific for p21 attenuate BMP-2-induced inhibition of proliferation when transfected into HASMCs, demonstrating that BMP-2 inhibits PDGF-stimulated proliferation of HASMCs through induction of p21. hasmcs 198-204 bone morphogenetic protein 2 Homo sapiens 59-64 12527559-5 2003 Antisense oligodeoxynucleotides specific for p21 attenuate BMP-2-induced inhibition of proliferation when transfected into HASMCs, demonstrating that BMP-2 inhibits PDGF-stimulated proliferation of HASMCs through induction of p21. hasmcs 198-204 bone morphogenetic protein 2 Homo sapiens 150-155 12534349-6 2003 In hASMCs, we showed that ERK activation depended on both pertussis-sensitive and -insensitive G alpha-proteins. hasmcs 3-9 mitogen-activated protein kinase 1 Homo sapiens 26-29 12410800-6 2002 SAA1 promoter transcription in cultured HASMCs was upregulated not by proinflammatory cytokines, but rather by glucocorticoids. hasmcs 40-46 serum amyloid A1 Homo sapiens 0-4 12577317-6 2003 In HASMCs, oxLDL induced IL-beta and IL-1ra expression and release in a dose- and time-dependent manner. hasmcs 3-9 interleukin-1 receptor antagonist protein Oryctolagus cuniculus 37-43 12577317-8 2003 Confocal microscopy showed colocalization of IL-beta and IL-1ra expression in oxLDL-stimulated HASMCs. hasmcs 95-101 interleukin-1 receptor antagonist protein Oryctolagus cuniculus 57-63 12577317-12 2003 This is the first demonstration of IL-1 beta and IL-1ra expression and secretion of oxLDL-treated HASMCs and their expression in the rabbit neointima, suggesting that the smooth muscle cells of the intima are an important source of these factors. hasmcs 98-104 interleukin-1 receptor antagonist protein Oryctolagus cuniculus 49-55 12410800-10 2002 The functional significance of the C/EBP site as indicated by the immunohistochemical result was that in HASMCs anti-C/EBPbeta reactivity was shifted from the cytoplasm to the nuclei. hasmcs 105-111 CCAAT enhancer binding protein alpha Homo sapiens 35-40 12410800-10 2002 The functional significance of the C/EBP site as indicated by the immunohistochemical result was that in HASMCs anti-C/EBPbeta reactivity was shifted from the cytoplasm to the nuclei. hasmcs 105-111 CCAAT enhancer binding protein beta Homo sapiens 117-126 12070119-7 2002 Adiponectin specifically bound to (125)I-PDGF-BB and significantly inhibited the association of (125)I-PDGF-BB with HASMCs, but no effects were observed on the binding of (125)I-PDGF-AA or (125)I-heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF) to HASMCs. hasmcs 116-122 adiponectin, C1Q and collagen domain containing Homo sapiens 0-11 12070119-7 2002 Adiponectin specifically bound to (125)I-PDGF-BB and significantly inhibited the association of (125)I-PDGF-BB with HASMCs, but no effects were observed on the binding of (125)I-PDGF-AA or (125)I-heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF) to HASMCs. hasmcs 273-279 adiponectin, C1Q and collagen domain containing Homo sapiens 0-11 11788700-10 2002 The expression of p44/42 MAPK was reduced for a treatment with 400 micromol/L flufenamic acid (controls, 427 BLU +/- 0.305 vs treatment group, 190 BLU +/- 106; P <.05) CONCLUSION: Flufenamic acid inhibits the proliferation and migration of haSMCs. hasmcs 243-249 interferon induced protein 44 Homo sapiens 18-21 11590167-5 2001 In this study, we demonstrate that the PPAR gamma natural ligand (15-deoxyprostaglandin J(2)) and a synthetic ligand (GW7845) significantly inhibit TGF-beta-induced CTGF production in a dose-dependent manner in HASMCs. hasmcs 211-217 peroxisome proliferator activated receptor gamma Homo sapiens 39-49 11590167-5 2001 In this study, we demonstrate that the PPAR gamma natural ligand (15-deoxyprostaglandin J(2)) and a synthetic ligand (GW7845) significantly inhibit TGF-beta-induced CTGF production in a dose-dependent manner in HASMCs. hasmcs 211-217 transforming growth factor beta 1 Homo sapiens 148-156 11590167-5 2001 In this study, we demonstrate that the PPAR gamma natural ligand (15-deoxyprostaglandin J(2)) and a synthetic ligand (GW7845) significantly inhibit TGF-beta-induced CTGF production in a dose-dependent manner in HASMCs. hasmcs 211-217 cellular communication network factor 2 Homo sapiens 165-169 11479257-5 2001 7E3 and eptifibatide inhibited alpha(v)beta(3)-mediated attachment of HASMCs to thrombospondin (TSP) and prothrombin but had no effect on alpha(v)beta(5)- or beta(1)-mediated HASMC attachment to vitronectin-, collagen-, or fibronectin-coated or noncoated tissue culture plates. hasmcs 70-76 thrombospondin 1 Homo sapiens 96-99 11479257-5 2001 7E3 and eptifibatide inhibited alpha(v)beta(3)-mediated attachment of HASMCs to thrombospondin (TSP) and prothrombin but had no effect on alpha(v)beta(5)- or beta(1)-mediated HASMC attachment to vitronectin-, collagen-, or fibronectin-coated or noncoated tissue culture plates. hasmcs 70-76 coagulation factor II, thrombin Homo sapiens 105-116 11479257-5 2001 7E3 and eptifibatide inhibited alpha(v)beta(3)-mediated attachment of HASMCs to thrombospondin (TSP) and prothrombin but had no effect on alpha(v)beta(5)- or beta(1)-mediated HASMC attachment to vitronectin-, collagen-, or fibronectin-coated or noncoated tissue culture plates. hasmcs 70-76 fibronectin 1 Homo sapiens 223-234 11479257-9 2001 In cell proliferation assays, eptifibatide inhibited alpha(v)beta(3)-mediated responses to soluble TSP by HASMCs and beta(3) integrin-expressing HEK cells. hasmcs 106-112 thrombospondin 1 Homo sapiens 99-102 10430178-5 1999 Conversely, IL-4 was a potent VCAM-1 inducer in HASMCs (ED50 approximately 100 pg/ml) but did not induce ICAM-1 expression. hasmcs 48-54 interleukin 4 Homo sapiens 12-16 11398146-6 2001 Finally, treatment of HASMCs with gliclazide resulted in a marked decrease in oxidatively modified LDL-induced monocyte chemoattractant protein (MCP)-1 and human heat shock protein 70 (hsp 70) expression, both at the gene and protein levels. hasmcs 22-28 C-C motif chemokine ligand 2 Homo sapiens 111-151 11398146-6 2001 Finally, treatment of HASMCs with gliclazide resulted in a marked decrease in oxidatively modified LDL-induced monocyte chemoattractant protein (MCP)-1 and human heat shock protein 70 (hsp 70) expression, both at the gene and protein levels. hasmcs 22-28 heat shock protein family A (Hsp70) member 4 Homo sapiens 162-183 11398146-6 2001 Finally, treatment of HASMCs with gliclazide resulted in a marked decrease in oxidatively modified LDL-induced monocyte chemoattractant protein (MCP)-1 and human heat shock protein 70 (hsp 70) expression, both at the gene and protein levels. hasmcs 22-28 heat shock protein family A (Hsp70) member 4 Homo sapiens 185-191 10590238-7 1999 OSM stimulated the release of IL-6 by hASMCs in a dose-dependent way; after a 48-hour exposure, values were 8.5+/-0.7, 29.7+/-3.5, 50.9+/-4.4, and 73.8+/-7.6x10(3) U/mL (n=6) at OSM concentrations of 0, 1, 10, and 100 ng/mL, respectively. hasmcs 38-44 interleukin 6 Homo sapiens 30-34 10564175-7 1999 These results provide evidence for a novel antiproliferative effect of NO and ANP in HASMCs mediated through cGMP-dependent and cGMP-independent mechanisms. hasmcs 85-91 natriuretic peptide A Homo sapiens 78-81 10430178-5 1999 Conversely, IL-4 was a potent VCAM-1 inducer in HASMCs (ED50 approximately 100 pg/ml) but did not induce ICAM-1 expression. hasmcs 48-54 vascular cell adhesion molecule 1 Homo sapiens 30-36 10213907-7 1999 In cellular migration studies, incubation with BMP-2 produced efficacious (</=610-fold), concentration- and time-dependent chemotaxis of HASMCs (EC50 = 0.8 microM) with little or no effect on HUVEC chemotaxis. hasmcs 140-146 bone morphogenetic protein 2 Homo sapiens 47-52 9048649-10 1997 Induction of E-selectin mRNA by lipopolysaccharide or TNF-alpha in cycloheximide-treated HASMCs was inhibited by the antioxidant pyrrolidinedithiocarbamate and the serine protease inhibitor N alpha-L-tosyl-L-phenylalanine chloromethyl ketone, suggesting that a nuclear factor-kappa B-like mechanism may play an important role in E-selectin gene expression in HASMCs. hasmcs 359-365 selectin E Homo sapiens 13-23 9361246-6 1997 Infected HASMCs uniformly increased their expression of MHC class I antigen by 55% +/- 21% above constitutive levels (assessed by flow cytometry (n = 5, p < 0.0001). hasmcs 9-15 MHC class I antigen Homo sapiens 56-75 9048649-8 1997 Furthermore, E-selectin was detected on the cell surface of HASMCs after washing out of cycloheximide. hasmcs 60-66 selectin E Homo sapiens 13-23 9048649-10 1997 Induction of E-selectin mRNA by lipopolysaccharide or TNF-alpha in cycloheximide-treated HASMCs was inhibited by the antioxidant pyrrolidinedithiocarbamate and the serine protease inhibitor N alpha-L-tosyl-L-phenylalanine chloromethyl ketone, suggesting that a nuclear factor-kappa B-like mechanism may play an important role in E-selectin gene expression in HASMCs. hasmcs 89-95 selectin E Homo sapiens 13-23 9048649-10 1997 Induction of E-selectin mRNA by lipopolysaccharide or TNF-alpha in cycloheximide-treated HASMCs was inhibited by the antioxidant pyrrolidinedithiocarbamate and the serine protease inhibitor N alpha-L-tosyl-L-phenylalanine chloromethyl ketone, suggesting that a nuclear factor-kappa B-like mechanism may play an important role in E-selectin gene expression in HASMCs. hasmcs 89-95 tumor necrosis factor Homo sapiens 54-63 9048649-10 1997 Induction of E-selectin mRNA by lipopolysaccharide or TNF-alpha in cycloheximide-treated HASMCs was inhibited by the antioxidant pyrrolidinedithiocarbamate and the serine protease inhibitor N alpha-L-tosyl-L-phenylalanine chloromethyl ketone, suggesting that a nuclear factor-kappa B-like mechanism may play an important role in E-selectin gene expression in HASMCs. hasmcs 89-95 selectin E Homo sapiens 329-339 9048649-10 1997 Induction of E-selectin mRNA by lipopolysaccharide or TNF-alpha in cycloheximide-treated HASMCs was inhibited by the antioxidant pyrrolidinedithiocarbamate and the serine protease inhibitor N alpha-L-tosyl-L-phenylalanine chloromethyl ketone, suggesting that a nuclear factor-kappa B-like mechanism may play an important role in E-selectin gene expression in HASMCs. hasmcs 359-365 tumor necrosis factor Homo sapiens 54-63 9048649-12 1997 Except for this repressor, HASMCs and endothelial cells may share similar regulatory mechanisms for controlling E-selectin expression. hasmcs 27-33 selectin E Homo sapiens 112-122 10608012-11 1995 These results suggest that (1) HASMCs can digest naturally produced ECM; (2) plasminogen-dependent mechanisms requiring cell contact are important in the initiation of this phenomenon; and (3) thrombin in the vicinity of clots may modulate the fibrinolytic and proteolytic properties of SMC through t-PA after vascular injury. hasmcs 31-37 plasminogen activator, tissue type Homo sapiens 299-303 8751615-11 1996 DISCUSSION: VEGF at 10 ng/ml combined with heparin at 50 units/ml exhibited maximal stimulation of HAECs with inhibition of HASMCs. hasmcs 124-130 vascular endothelial growth factor A Homo sapiens 12-16 8230860-4 1993 The addition of AII to HASMCs induced a rapid, transient increase in intracellular free Ca2+ concentration followed by a lower, sustained phase. hasmcs 23-29 angiotensinogen Homo sapiens 16-19 10608012-8 1995 Baseline secretion of tissue-type plasminogen activator (t-PA) into the media by HASMCs averaged 3.9 ng/105 cells/24 hr and baseline secretion of type-1 plasminogen activator inhibitor (PAI-1) averaged 1300 ng/105 cells/24 hr. hasmcs 81-87 plasminogen activator, tissue type Homo sapiens 22-55 10608012-8 1995 Baseline secretion of tissue-type plasminogen activator (t-PA) into the media by HASMCs averaged 3.9 ng/105 cells/24 hr and baseline secretion of type-1 plasminogen activator inhibitor (PAI-1) averaged 1300 ng/105 cells/24 hr. hasmcs 81-87 plasminogen activator, tissue type Homo sapiens 57-61 10608012-11 1995 These results suggest that (1) HASMCs can digest naturally produced ECM; (2) plasminogen-dependent mechanisms requiring cell contact are important in the initiation of this phenomenon; and (3) thrombin in the vicinity of clots may modulate the fibrinolytic and proteolytic properties of SMC through t-PA after vascular injury. hasmcs 31-37 coagulation factor II, thrombin Homo sapiens 193-201 8107324-6 1993 We also studied the effect of ET-1 on the cytosolic [Ca2+]i in HASMCs loaded with fura-2/AM. hasmcs 63-69 endothelin 1 Homo sapiens 30-34 8230860-6 1993 These results revealed AII receptors in HASMCs to be of the type 1 receptor subtype, which induce Ca2+ mobilization mainly from intracellular Ca2+ stores. hasmcs 40-46 angiotensinogen Homo sapiens 23-26