PMID-sentid Pub_year Sent_text compound_name comp_offset prot_official_name organism prot_offset 11085540-5 2000 Unexpectedly, this decrease in cell number reflected bFGF-induced apoptosis and necrosis, as demonstrated by electron microscopy, binding of annexin V, and staining with acridine orange. Acridine Orange 170-185 fibroblast growth factor 2 Mus musculus 53-57 11509277-10 2001 The END cTnI level for the AO-group was 0.91+/-0.5 ng/l; for all other patients, this was 0.37+/-0.3 ng/l (P<0.001). Acridine Orange 27-29 troponin I3, cardiac type Homo sapiens 8-12 11295227-5 2001 Immunocytochemistry and vital staining with acridine orange revealed that GCD-10 was localized at the perinuclear area of cultured microglia and COS 1 cells infected with a GCD-10-expressing adenoviral vector. Acridine Orange 44-59 lysosomal protein transmembrane 5 Rattus norvegicus 74-80 11239247-4 2001 Cell cycle changes in cells overexpressing PKCalpha or PKCgamma were measured using acridine orange staining and flow cytometry. Acridine Orange 84-99 protein kinase C alpha type Oryctolagus cuniculus 43-51 11223953-6 2001 The early stage 12 is characterized by an asynchronous nurse cell nuclear chromatin condensation, while at late stage 12 the actin cables become very thick, as adjacent ones overlap one another and traverse the disorganized apoptotic nurse cell nuclei that already have fragmented DNA, as is demonstrated by acridine orange staining and TUNEL assay. Acridine Orange 308-323 Actin 79B Drosophila melanogaster 125-130 11162237-6 2001 To further support the role of lysosomal membrane damage in Abeta-mediated cell death, we demonstrate that photodisruption of acridine orange (AO)-loaded lysosomes with intense blue light induces a relatively rapid synchronous lysosomal membrane damage and neuronal death similar to that observed as a result of Abeta exposure. Acridine Orange 126-141 amyloid beta precursor protein Homo sapiens 60-65 11162237-6 2001 To further support the role of lysosomal membrane damage in Abeta-mediated cell death, we demonstrate that photodisruption of acridine orange (AO)-loaded lysosomes with intense blue light induces a relatively rapid synchronous lysosomal membrane damage and neuronal death similar to that observed as a result of Abeta exposure. Acridine Orange 126-141 amyloid beta precursor protein Homo sapiens 312-317 11162237-6 2001 To further support the role of lysosomal membrane damage in Abeta-mediated cell death, we demonstrate that photodisruption of acridine orange (AO)-loaded lysosomes with intense blue light induces a relatively rapid synchronous lysosomal membrane damage and neuronal death similar to that observed as a result of Abeta exposure. Acridine Orange 143-145 amyloid beta precursor protein Homo sapiens 60-65 11162237-6 2001 To further support the role of lysosomal membrane damage in Abeta-mediated cell death, we demonstrate that photodisruption of acridine orange (AO)-loaded lysosomes with intense blue light induces a relatively rapid synchronous lysosomal membrane damage and neuronal death similar to that observed as a result of Abeta exposure. Acridine Orange 143-145 amyloid beta precursor protein Homo sapiens 312-317 11150636-4 2000 We tested whether antisense phosphorothiolated oligonucleotides (AO) directed at the Abeta region of the APP gene given with or without antibody directed at Abeta could reverse the elevated protein levels of APP and the behavioral impairments seen in SAMP8 mice, a strain which spontaneously overexpresses APP. Acridine Orange 65-67 amyloid beta (A4) precursor protein Mus musculus 85-90 11313959-8 2001 Further studies using acridine orange and ethidium bromide to measure apoptosis revealed that mdr1b caused apoptosis and this was enhanced by p53, however the increased apoptosis required a functional p53 transactivation domain. Acridine Orange 22-37 ATP-binding cassette, sub-family B (MDR/TAP), member 1B Mus musculus 94-99 11069115-9 2000 Simultaneous observations of phogrin-EGFP and acridine orange indicated that the decrease in EGFP fluorescence occurred at the time of the acridine orange release, and that the lateral movement of EGFP-expressing vesicles occurred after this. Acridine Orange 139-154 protein tyrosine phosphatase, receptor type N2 Rattus norvegicus 29-36 9749934-0 1998 Intrahepatic flow disturbance as detected by in vivo acridine orange staining in endothelin-1-treated and cirrhotic rats. Acridine Orange 53-68 endothelin 1 Rattus norvegicus 81-93 10952539-4 2000 In addition, the adsorption of acridine orange on silanol groups on a glass surface could be monitored directly by TIR-TLS, and the adsorption isotherm agreed well with Langmuir"s model. Acridine Orange 31-46 FUS RNA binding protein Homo sapiens 119-122 10515125-1 1999 When cultured NIT-1 cells were subjected to a low level of oxidative stress (30 microM hydrogen peroxide for 15 min at 37 degrees C) several of their lysosomes ruptured, as demonstrated by intravital staining with the lysosomotropic weak base acridine orange. Acridine Orange 243-258 nitrilase 1 Mus musculus 14-19 10100720-4 1999 Our results show a strong correlation between percentage of PARP cleavage and percentage of acridine orange-positive cells in colon cancer cell lines treated with 0.1 microM TPT for 24 and 48 h, confirming that PARP cleavage is a useful marker for programmed cell death in colon cancer cell lines. Acridine Orange 92-107 poly(ADP-ribose) polymerase 1 Homo sapiens 60-64 10100720-4 1999 Our results show a strong correlation between percentage of PARP cleavage and percentage of acridine orange-positive cells in colon cancer cell lines treated with 0.1 microM TPT for 24 and 48 h, confirming that PARP cleavage is a useful marker for programmed cell death in colon cancer cell lines. Acridine Orange 92-107 poly(ADP-ribose) polymerase 1 Homo sapiens 211-215 18967427-0 1998 Investigation of the interaction between acridine orange and bovine serum albumin. Acridine Orange 41-56 albumin Homo sapiens 68-81 18967427-1 1998 The results from the measurement of the fluorescence spectrum showing the binding characteristics of acridine orange (AO) and bovine serum albumin (BSA) are reported. Acridine Orange 101-116 albumin Homo sapiens 133-146 18967427-1 1998 The results from the measurement of the fluorescence spectrum showing the binding characteristics of acridine orange (AO) and bovine serum albumin (BSA) are reported. Acridine Orange 118-120 albumin Homo sapiens 133-146 11017877-7 2000 Acridine Orange staining revealed an increase in lysosomal content with replicative age, which correlated with the increase in (beta)-galactosidase. Acridine Orange 0-15 galactosidase beta 1 Homo sapiens 128-147 10804293-2 2000 In this study, we undertook to clarify the effect of photodynamic therapy (PDT) with acridine orange (AO) on the MDR mouse osteosarcoma (MOS / ADR1) cell line, by comparing the outcome with the effect on a chemosensitive osteosarcoma (MOS) cell line. Acridine Orange 85-100 Moloney sarcoma oncogene Mus musculus 137-140 10804293-2 2000 In this study, we undertook to clarify the effect of photodynamic therapy (PDT) with acridine orange (AO) on the MDR mouse osteosarcoma (MOS / ADR1) cell line, by comparing the outcome with the effect on a chemosensitive osteosarcoma (MOS) cell line. Acridine Orange 85-100 Moloney sarcoma oncogene Mus musculus 235-238 10804293-2 2000 In this study, we undertook to clarify the effect of photodynamic therapy (PDT) with acridine orange (AO) on the MDR mouse osteosarcoma (MOS / ADR1) cell line, by comparing the outcome with the effect on a chemosensitive osteosarcoma (MOS) cell line. Acridine Orange 102-104 Moloney sarcoma oncogene Mus musculus 137-140 10804293-2 2000 In this study, we undertook to clarify the effect of photodynamic therapy (PDT) with acridine orange (AO) on the MDR mouse osteosarcoma (MOS / ADR1) cell line, by comparing the outcome with the effect on a chemosensitive osteosarcoma (MOS) cell line. Acridine Orange 102-104 Moloney sarcoma oncogene Mus musculus 235-238 10804293-6 2000 Even after flash exposure to AO at concentrations above 1.0 microg/ml plus 1-min illumination, the viability of MOS/ADR1 cells decreased to a viability ratio of less than 1/ 1000 within 72 h. Based on these results, we concluded that AO with photo-excitation has a strong cytocidal effect, not only on chemosensitive mouse osteosarcoma cells, but also on MDR mouse osteosarcoma cells. Acridine Orange 29-31 Moloney sarcoma oncogene Mus musculus 112-115 9295025-4 1997 In vitro acridine orange staining indicated that most of the rapidly accumulating memory phenotype CD4+ T cells of 4-month-old male BXSB mice are G1 arrested. Acridine Orange 9-24 CD4 antigen Mus musculus 99-102 9292533-5 1997 Moreover, inhibition of MDM2 using antisense ODNs also triggered MM cell apoptosis as evidenced by acridine orange-ethidium bromide staining. Acridine Orange 99-114 MDM2 proto-oncogene Homo sapiens 24-28 9614205-1 1998 To investigate alpha1B-adrenoceptor function, we developed a phosphorothioate antisense oligodeoxynucleotide (AO) to inhibit the expression of the alpha1B-adrenoceptor subtype in DDT1 MF2 cells. Acridine Orange 110-112 alpha-1B adrenergic receptor Mesocricetus auratus 15-35 9614205-1 1998 To investigate alpha1B-adrenoceptor function, we developed a phosphorothioate antisense oligodeoxynucleotide (AO) to inhibit the expression of the alpha1B-adrenoceptor subtype in DDT1 MF2 cells. Acridine Orange 110-112 alpha-1B adrenergic receptor Mesocricetus auratus 147-167 9614205-6 1998 In time course experiments, AO (10 microM) reduced alpha1B-adrenoceptor density by 28, 64, and 68% versus controls after 24, 48, and 72 hr of exposure, respectively. Acridine Orange 28-30 alpha-1B adrenergic receptor Mesocricetus auratus 51-71 9614205-7 1998 alpha1B-Adrenoceptor mRNA concentration (measured by RT-PCR) was reduced by 25% in cells treated for 48 hr with 10 microM AO versus controls. Acridine Orange 122-124 alpha-1B adrenergic receptor Mesocricetus auratus 0-20 9415270-8 1997 In contrast, after the spasm the plasma soluble ICAM-1 level was (P < .05) higher in CS than AO and the CS-AO differences of soluble ICAM-1 (P < .05) and VCAM-1 (P < .05) increased as compared with the baseline, respectively. Acridine Orange 96-98 intercellular adhesion molecule 1 Homo sapiens 48-54 9201618-3 1997 Simultaneous exposure of endothelial cells to VEGF resulted in a dose dependent inhibition of apoptosis when evaluated by: (1) direct counting of cells with morphologic features of apoptosis after acridine orange staining; (2) analysis of DNA fragmentation by (a) agarose gel electrophoresis and (b) fluorescence activated cell sorting (FACS); and (3) viability assays dependent upon mitochondrial function. Acridine Orange 197-212 vascular endothelial growth factor A Homo sapiens 46-50 9171939-7 1997 Reverse transcriptase polymerase chain reaction analysis showed that AO induced a 2-fold increase in the levels of c-fos and p53 transcripts in comparison to RO which had no significant effect. Acridine Orange 69-71 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 115-120 9171939-7 1997 Reverse transcriptase polymerase chain reaction analysis showed that AO induced a 2-fold increase in the levels of c-fos and p53 transcripts in comparison to RO which had no significant effect. Acridine Orange 69-71 tumor protein p53 Homo sapiens 125-128 9056256-6 1997 When erythrocytes were pretreated with endo-beta-galactosidase to remove poly-N-acetyllactosaminyl saccharide chains from cell surface prior to acridine orange treatment, the cells did not become susceptible to anti-band 3 binding. Acridine Orange 144-159 galactosidase beta 1 Homo sapiens 44-62 27406671-6 1996 Genistein, sphingosine and acridine orange have been shown to prevent insulin-stimulated ferricyanide reduction, implicating tyrosine phosphorylation as an important signal for activation of the enzyme by insulin. Acridine Orange 27-42 insulin Homo sapiens 70-77 27406671-6 1996 Genistein, sphingosine and acridine orange have been shown to prevent insulin-stimulated ferricyanide reduction, implicating tyrosine phosphorylation as an important signal for activation of the enzyme by insulin. Acridine Orange 27-42 insulin Homo sapiens 205-212 7490476-3 1995 After IFN-gamma treatment in the presence of anti-Fas monoclonal antibody, apoptosis was induced, as detected by the formation of nucleosome-sized fragments of DNA and morphologically by apoptotic cells with round homogeneous nuclear beads detected by acridine orange staining. Acridine Orange 252-267 interferon gamma Homo sapiens 6-15 8809131-7 1996 In this study we demonstrate that acridine orange stimulation of an IgG3 anti-C. neoformans-producing hybridoma can be used to recover the entire set of isotype switch variants: IgGl, IgG2b, IgG2a, IgE and IgA. Acridine Orange 34-49 immunoglobulin heavy constant gamma 3 (G3m marker) Homo sapiens 68-72 8809131-7 1996 In this study we demonstrate that acridine orange stimulation of an IgG3 anti-C. neoformans-producing hybridoma can be used to recover the entire set of isotype switch variants: IgGl, IgG2b, IgG2a, IgE and IgA. Acridine Orange 34-49 immunoglobulin heavy constant epsilon Homo sapiens 198-201 7662729-1 1995 The platelet release reaction induced by thrombin was studied by means of the kinetic fluorometric method with usage of the fluorescent probe, acridine orange, and the platelet aggregation was measured with Born"s turbidometric method. Acridine Orange 143-158 coagulation factor II, thrombin Homo sapiens 41-49 7807995-2 1994 The results demonstrated that an augmented cell death occurred with TF-1 cells when pre-incubated for 24 h with IL-3 followed by treatment with VP-16 (10 micrograms/ml) for 1 h. The increased cell death could not be ascribed to an increased number of apoptotic cells as measured with the acridine orange method. Acridine Orange 288-303 interleukin 3 Homo sapiens 112-116 7807995-2 1994 The results demonstrated that an augmented cell death occurred with TF-1 cells when pre-incubated for 24 h with IL-3 followed by treatment with VP-16 (10 micrograms/ml) for 1 h. The increased cell death could not be ascribed to an increased number of apoptotic cells as measured with the acridine orange method. Acridine Orange 288-303 host cell factor C1 Homo sapiens 144-149 7930604-7 1994 Fluorescent microscopic observation of OE4 stained with acridine orange indicated that CMA raised the pH of the lytic granules. Acridine Orange 56-71 EBF family member 4 Homo sapiens 39-42 7913684-1 1994 This study explores properties of P-glycoprotein dependent membrane transport in rat liver with the use of acridine orange as the substrate. Acridine Orange 107-122 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 34-48 7913684-5 1994 Binding of acridine orange to liver P-glycoprotein was analyzed by photoaffinity labeling with azidopine, a substrate of P-glycoprotein dependent transport in multi-drug resistant tumor cells. Acridine Orange 11-26 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 36-50 7913684-5 1994 Binding of acridine orange to liver P-glycoprotein was analyzed by photoaffinity labeling with azidopine, a substrate of P-glycoprotein dependent transport in multi-drug resistant tumor cells. Acridine Orange 11-26 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 121-135 7913684-6 1994 Labeling of the immunoprecipitated P-glycoprotein was inhibited by acridine orange, verapamil, and by cyclosporin A. Acridine Orange 67-82 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 35-49 7913684-7 1994 The results show that biliary secretion of acridine orange is highly analogous to P-glycoprotein mediated membrane drug transport in tumor cells that exhibit multi-drug resistance. Acridine Orange 43-58 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 82-96 8110987-4 1993 Acetylcholinesterase (AChE) was inhibited by CPZ, 3,7,8-trihydroxy-CPZ, acridine orange partially saturated desipramine, imipramine, trans-clopenthixol and tetrahydrocannabidiolic at 10(-4) to 10(-5). Acridine Orange 72-87 acetylcholinesterase Rattus norvegicus 0-20 7909288-8 1994 A statistical correlation was found between overall PCNA positivity and RNA content as determined by acridine orange analysis. Acridine Orange 101-116 proliferating cell nuclear antigen Homo sapiens 52-56 8110987-4 1993 Acetylcholinesterase (AChE) was inhibited by CPZ, 3,7,8-trihydroxy-CPZ, acridine orange partially saturated desipramine, imipramine, trans-clopenthixol and tetrahydrocannabidiolic at 10(-4) to 10(-5). Acridine Orange 72-87 acetylcholinesterase Rattus norvegicus 22-26 8110987-5 1993 A metalloproteinase, MMP-7-ase, was inhibited by tetrahydrocannabidiolic acid, 3,7,8-trihydroxy-CPZ, acridine orange but other tissue proteinases, ATN-ase and cathepsin B, were less sensitive to these compounds. Acridine Orange 101-116 cathepsin B Rattus norvegicus 159-170 1567389-2 1992 [Ca2+]i was increased by gastrin (100 nM) in approximately 30% of the parietal cells, which were identified by using either the fluorescent probe acridine orange or a parietal cell-specific monoclonal antibody. Acridine Orange 146-161 gastrin Rattus norvegicus 25-32 9874748-8 1992 Acridine Orange partitions into acidic compartments and might be expected to be concentrated in goblet cavities, as these are the compartments toward which the V-ATPase pumps protons. Acridine Orange 0-15 Vacuolar H[+]-ATPase SFD subunit Drosophila melanogaster 160-168 1533571-5 1992 Acridine orange analysis of sorted CD44hi/lo fractions revealed that the diploid (G1) population of the CD44hi T cells displayed a higher mean RNA content than the CD44lo T cells. Acridine Orange 0-15 CD44 molecule (Indian blood group) Homo sapiens 35-39 1533571-5 1992 Acridine orange analysis of sorted CD44hi/lo fractions revealed that the diploid (G1) population of the CD44hi T cells displayed a higher mean RNA content than the CD44lo T cells. Acridine Orange 0-15 CD44 molecule (Indian blood group) Homo sapiens 104-108 1533571-5 1992 Acridine orange analysis of sorted CD44hi/lo fractions revealed that the diploid (G1) population of the CD44hi T cells displayed a higher mean RNA content than the CD44lo T cells. Acridine Orange 0-15 CD44 molecule (Indian blood group) Homo sapiens 104-108 1832051-6 1991 Acridine orange analysis established that TGF-beta inhibits the LPS-induced B cell transition from G0 to G1A phase of the cell cycle. Acridine Orange 0-15 transforming growth factor, beta 1 Mus musculus 42-50 2004666-8 1991 The differential rise in nuclear Ca2+ was eliminated by acridine orange binding to nucleic acids. Acridine Orange 56-71 carbonic anhydrase 2 Homo sapiens 33-36 1827137-3 1991 Inasmuch as processing of antigens requires their passage through acidic compartments, we undertook the present study to examine the ability of fLC and cLC to take up acridine orange, and to identify proton-translocating ATPases in these cells. Acridine Orange 167-182 Charcot-Leyden crystal galectin Homo sapiens 152-155 1827137-4 1991 Using flow cytometry and fluorescence microscopy, acridine orange was observed to accumulate in acidic compartments in both fLC and cLC. Acridine Orange 50-65 Charcot-Leyden crystal galectin Homo sapiens 132-135 1957650-6 1991 Acridine orange fluorochrome distinguished vimentin/desmin-reactive myofibres that were regenerating from those of developmental myopathies because the RNA fluorescence was strong in regenerating myofibres and in fetal myotubes, but was absent from myofibres in developmental disorders of muscle. Acridine Orange 0-15 vimentin Homo sapiens 43-51 1957650-6 1991 Acridine orange fluorochrome distinguished vimentin/desmin-reactive myofibres that were regenerating from those of developmental myopathies because the RNA fluorescence was strong in regenerating myofibres and in fetal myotubes, but was absent from myofibres in developmental disorders of muscle. Acridine Orange 0-15 desmin Homo sapiens 52-58 2195545-1 1990 Ethidium bromide, acridine orange, 4"-(9-acridinylamino)methanesulfon-o-anisidide (o-AMSA), and m-AMSA induce the rapid binding of RecA protein to double-stranded (ds) DNA. Acridine Orange 18-33 RAD51 recombinase Homo sapiens 131-135 2195545-4 1990 Ethidium bromide, acridine orange, and quinacrine inhibit RecA protein binding to single-stranded DNA. Acridine Orange 18-33 RAD51 recombinase Homo sapiens 58-62 34580807-5 2021 Western blot analyses of ERK1/2 and autophagic markers (LC3, SQSTM1), and cellular staining with acridine orange showed that ERK1/2 and autophagic activities both decreased with time in culture. Acridine Orange 97-112 mitogen-activated protein kinase 3 Homo sapiens 125-131 34347236-3 2021 Effect of lgals3a-e3i3-MO on apoptosis of zebrafish was detected by acridine orange staining. Acridine Orange 68-83 lectin, galactoside binding soluble 3a Danio rerio 10-17 34347236-7 2021 The results of acridine orange staining showed that compared with the control-MO group, lgals3a-e3i3-MO promoted cardiomyocyte apoptosis in zebrafish. Acridine Orange 15-30 lectin, galactoside binding soluble 3a Danio rerio 88-95 34481879-1 2021 This study evaluated the potential of antitumor activity of snake venom from Vipera ammodytes and L-amino acid oxidase from Crotalus adamanteus on different colorectal cancer cell lines through determination of cytotoxic activity by MTT assay, pro-apoptotic activity by acridine orange/ethidium bromide staining, and concentrations of redox status parameters (superoxide, reduced glutathione, lipid peroxidation) by colorimetric methods. Acridine Orange 270-285 interleukin 4 induced 1 Homo sapiens 98-118 34523169-4 2021 AO exposure significantly increases the expressions of beige-specific genes (Cidea, Cited1, Ppargc1alpha, Prdm16, Tbx1, Tmem26, and Ucp1) and brown-fat signature proteins (UCP1, PRDM16, and PGC-1alpha), and suppresses the expressions of lipogenic marker proteins while enhancing the protein levels of lipolysis in white adipocytes. Acridine Orange 0-2 cell death inducing DFFA like effector a Homo sapiens 77-82 34523169-4 2021 AO exposure significantly increases the expressions of beige-specific genes (Cidea, Cited1, Ppargc1alpha, Prdm16, Tbx1, Tmem26, and Ucp1) and brown-fat signature proteins (UCP1, PRDM16, and PGC-1alpha), and suppresses the expressions of lipogenic marker proteins while enhancing the protein levels of lipolysis in white adipocytes. Acridine Orange 0-2 Cbp/p300 interacting transactivator with Glu/Asp rich carboxy-terminal domain 1 Homo sapiens 84-90 34523169-4 2021 AO exposure significantly increases the expressions of beige-specific genes (Cidea, Cited1, Ppargc1alpha, Prdm16, Tbx1, Tmem26, and Ucp1) and brown-fat signature proteins (UCP1, PRDM16, and PGC-1alpha), and suppresses the expressions of lipogenic marker proteins while enhancing the protein levels of lipolysis in white adipocytes. Acridine Orange 0-2 PPARG coactivator 1 alpha Homo sapiens 92-104 34523169-4 2021 AO exposure significantly increases the expressions of beige-specific genes (Cidea, Cited1, Ppargc1alpha, Prdm16, Tbx1, Tmem26, and Ucp1) and brown-fat signature proteins (UCP1, PRDM16, and PGC-1alpha), and suppresses the expressions of lipogenic marker proteins while enhancing the protein levels of lipolysis in white adipocytes. Acridine Orange 0-2 PR/SET domain 16 Homo sapiens 106-112 34523169-4 2021 AO exposure significantly increases the expressions of beige-specific genes (Cidea, Cited1, Ppargc1alpha, Prdm16, Tbx1, Tmem26, and Ucp1) and brown-fat signature proteins (UCP1, PRDM16, and PGC-1alpha), and suppresses the expressions of lipogenic marker proteins while enhancing the protein levels of lipolysis in white adipocytes. Acridine Orange 0-2 T-box transcription factor 1 Homo sapiens 114-118 34523169-4 2021 AO exposure significantly increases the expressions of beige-specific genes (Cidea, Cited1, Ppargc1alpha, Prdm16, Tbx1, Tmem26, and Ucp1) and brown-fat signature proteins (UCP1, PRDM16, and PGC-1alpha), and suppresses the expressions of lipogenic marker proteins while enhancing the protein levels of lipolysis in white adipocytes. Acridine Orange 0-2 transmembrane protein 26 Homo sapiens 120-126 34523169-4 2021 AO exposure significantly increases the expressions of beige-specific genes (Cidea, Cited1, Ppargc1alpha, Prdm16, Tbx1, Tmem26, and Ucp1) and brown-fat signature proteins (UCP1, PRDM16, and PGC-1alpha), and suppresses the expressions of lipogenic marker proteins while enhancing the protein levels of lipolysis in white adipocytes. Acridine Orange 0-2 uncoupling protein 1 Homo sapiens 132-136 34523169-4 2021 AO exposure significantly increases the expressions of beige-specific genes (Cidea, Cited1, Ppargc1alpha, Prdm16, Tbx1, Tmem26, and Ucp1) and brown-fat signature proteins (UCP1, PRDM16, and PGC-1alpha), and suppresses the expressions of lipogenic marker proteins while enhancing the protein levels of lipolysis in white adipocytes. Acridine Orange 0-2 uncoupling protein 1 Homo sapiens 172-176 34523169-4 2021 AO exposure significantly increases the expressions of beige-specific genes (Cidea, Cited1, Ppargc1alpha, Prdm16, Tbx1, Tmem26, and Ucp1) and brown-fat signature proteins (UCP1, PRDM16, and PGC-1alpha), and suppresses the expressions of lipogenic marker proteins while enhancing the protein levels of lipolysis in white adipocytes. Acridine Orange 0-2 PR/SET domain 16 Homo sapiens 178-184 34523169-4 2021 AO exposure significantly increases the expressions of beige-specific genes (Cidea, Cited1, Ppargc1alpha, Prdm16, Tbx1, Tmem26, and Ucp1) and brown-fat signature proteins (UCP1, PRDM16, and PGC-1alpha), and suppresses the expressions of lipogenic marker proteins while enhancing the protein levels of lipolysis in white adipocytes. Acridine Orange 0-2 PPARG coactivator 1 alpha Homo sapiens 190-200 26628983-6 2015 Acridine orange staining and MDC staining showed that Interferon-lambda1 triggered the accumulation of acidic vesicular and autolysosomes in osteosarcoma cell. Acridine Orange 0-15 interferon lambda 1 Homo sapiens 54-72 34314307-4 2021 METHODS: The cytotoxic and apoptotic effects of indoximod alone or IFN-gamma or TNF-alpha induction to mimic an inflammatory environment were evaluated by WST-1, Annexin V, cell cycle analysis, and acridine orange (AO)/ethidium bromide (EtBr) staining. Acridine Orange 198-213 tumor necrosis factor Homo sapiens 80-89 34314307-4 2021 METHODS: The cytotoxic and apoptotic effects of indoximod alone or IFN-gamma or TNF-alpha induction to mimic an inflammatory environment were evaluated by WST-1, Annexin V, cell cycle analysis, and acridine orange (AO)/ethidium bromide (EtBr) staining. Acridine Orange 215-217 tumor necrosis factor Homo sapiens 80-89 34439193-7 2021 In this work, we used AS1411-AuNPs as a carrier for an acridine orange derivative (C8) or Imiquimod (IQ). Acridine Orange 55-70 homeobox C8 Homo sapiens 82-86 2525477-7 1989 Analysis of the cell-cycle progression of SAC and BSF-MP6 plus IL 2 and IL 4-stimulated cells by acridine orange staining and fluorescence-activated cell sorter (FACS) analysis demonstrated an arrest of a minor cell population in G0 and a block of the transition of G1 cells to S phase. Acridine Orange 97-112 interleukin 2 Homo sapiens 63-67 34994761-4 2022 The results showed that AO supplementation avoided the elevation of blood pressure induced by the HS diet in SS rats, increased the renal antioxidant enzyme activities (catalase, superoxide dismutase, glutathione reductase, and glutathione S-transferase), reduced the H2O2 and MDA levels, and restored the normal antioxidant status of the serum and kidneys. Acridine Orange 24-26 catalase Rattus norvegicus 169-177 34994761-4 2022 The results showed that AO supplementation avoided the elevation of blood pressure induced by the HS diet in SS rats, increased the renal antioxidant enzyme activities (catalase, superoxide dismutase, glutathione reductase, and glutathione S-transferase), reduced the H2O2 and MDA levels, and restored the normal antioxidant status of the serum and kidneys. Acridine Orange 24-26 glutathione-disulfide reductase Rattus norvegicus 201-222 34994761-4 2022 The results showed that AO supplementation avoided the elevation of blood pressure induced by the HS diet in SS rats, increased the renal antioxidant enzyme activities (catalase, superoxide dismutase, glutathione reductase, and glutathione S-transferase), reduced the H2O2 and MDA levels, and restored the normal antioxidant status of the serum and kidneys. Acridine Orange 24-26 hematopoietic prostaglandin D synthase Rattus norvegicus 228-253 34095664-0 2021 Natural Molybdenite- and Tyrosinase-Based Amperometric Catechol Biosensor Using Acridine Orange as a Glue, Anchor, and Stabilizer for the Adsorbed Tyrosinase. Acridine Orange 80-95 tyrosinase Homo sapiens 147-157 34095664-2 2021 The developed TYR/AO/MLN-GCE-based amperometric TYR biosensor exhibited excellent performance for highly sensitive determination of catechol (linear range, 0.1-80 muM; sensitivity, 0.0315 muA/muM; LOD, 0.029 muM; response time, <4 s) with good reproducibility and good operational and storage stabilities. Acridine Orange 18-20 tyrosinase Homo sapiens 14-17 34095664-2 2021 The developed TYR/AO/MLN-GCE-based amperometric TYR biosensor exhibited excellent performance for highly sensitive determination of catechol (linear range, 0.1-80 muM; sensitivity, 0.0315 muA/muM; LOD, 0.029 muM; response time, <4 s) with good reproducibility and good operational and storage stabilities. Acridine Orange 18-20 tyrosinase Homo sapiens 48-51 35415679-5 2022 Additionally, the exposure of AGS and SGC7901 to FIP-nha induced autophagy as indicated by western blot analysis, GFP-LC3 and mCherry-GFP-LC3 transfection and acridine orange staining. Acridine Orange 159-174 upstream transcription factor 2, c-fos interacting Homo sapiens 49-52 35277054-6 2022 The activation of adiponectin and FXR expression in hepatic tissue may be a major mechanistic signaling cascade supporting the promising role of AO in NAFLD pharmacotherapy. Acridine Orange 145-147 adiponectin, C1Q and collagen domain containing Mus musculus 18-29 2525477-7 1989 Analysis of the cell-cycle progression of SAC and BSF-MP6 plus IL 2 and IL 4-stimulated cells by acridine orange staining and fluorescence-activated cell sorter (FACS) analysis demonstrated an arrest of a minor cell population in G0 and a block of the transition of G1 cells to S phase. Acridine Orange 97-112 interleukin 4 Homo sapiens 72-76 2646154-0 1989 Acridine orange, an inhibitor of protein kinase C, abolishes insulin and growth hormone stimulation of lipogenesis in rat adipocytes. Acridine Orange 0-15 gonadotropin releasing hormone receptor Rattus norvegicus 73-87 2565619-8 1989 Irradiation of acridine orange-loaded lysosomes (light intensity at the sample position approximately 320 mW/cm2) produces an impairment of the membrane which leads to a rapid release of enzyme (N-acetyl-beta-glucosaminidase activity) into the medium, accompanied by a loss of activity in the lysosome-containing pellet and a partial photodamage of the enzyme. Acridine Orange 15-30 O-GlcNAcase Homo sapiens 195-224 2995342-2 1985 Microsomes were enriched in the enzyme marker gamma-glutamyl transferase and contained an ATP-dependent proton pump, as evidenced by ATP-dependent, 3,3",4",5-tetrachlorosalicylanilide-reversible quenching of acridine orange fluorescence. Acridine Orange 208-223 gamma-glutamyltransferase 1 Rattus norvegicus 46-72 3450479-9 1987 The mechanism of the cytotoxic action of TNF was examined using a microspectrophotometric assay for the lysosomotropic probe, acridine orange. Acridine Orange 126-141 tumor necrosis factor Homo sapiens 41-44 3734584-6 1986 The pHi was estimated by using the pH probes carbon 14-labeled 5,5"-dimethyloxazolidinedione and acridine orange. Acridine Orange 97-112 glucose-6-phosphate isomerase Rattus norvegicus 4-7 2873034-9 1986 In the presence of valinomycin and an outward-directed K gradient, there was increased quenching of acridine orange, indicating that the H+-ATPase is electrogenic. Acridine Orange 100-115 dynein axonemal heavy chain 8 Homo sapiens 137-146 3937590-4 1985 Because increased fluorescence of acridine orange is a sign of greater acidity, these results suggest that (1) PTH stimulates the acidity of osteoclasts, (2) carbonic anhydrase activity is necessary for maximum acidity, and (3) carbonic anhydrase is activated by PTH. Acridine Orange 34-49 parathyroid hormone Homo sapiens 111-114 3937590-4 1985 Because increased fluorescence of acridine orange is a sign of greater acidity, these results suggest that (1) PTH stimulates the acidity of osteoclasts, (2) carbonic anhydrase activity is necessary for maximum acidity, and (3) carbonic anhydrase is activated by PTH. Acridine Orange 34-49 parathyroid hormone Homo sapiens 263-266 3258596-15 1988 Acridine orange and acridine yellow G also inhibited thrombin-induced 40-kDa phosphorylation in human platelets and phorbol dibutyrate binding to platelets. Acridine Orange 0-15 coagulation factor II, thrombin Homo sapiens 53-61 3671676-2 1987 The changes were similar to those caused by PHA that is: the increase in acridine-orange binding to DNA during the first 45-90 min, its fall to the control level in 3-4 h and the subsequent increase. Acridine Orange 73-88 lamin B receptor Homo sapiens 44-47 3765107-6 1986 NR, AO and NH4+ accumulation in cells resulted in inhibition of the activity of the following lysosomal hydrolases: cathepsins B and D, acid lipase, N-acetyl-beta,D-glucosaminidase, beta-galactosidase, acid phosphatase and galactosyltransferase, the latter being a marker of Golgi apparatus. Acridine Orange 4-6 galactosidase beta 1 Homo sapiens 182-200 2419244-2 1985 Flow cytometric cell cycle analyses of acridine orange-stained cells showed that CS-A (0.5 micrograms/ml) inhibits the G0-G1 activation process of a substantial proportion of PHA- and Con A-stimulated lymphocytes. Acridine Orange 39-54 heat shock protein 9 Mus musculus 81-85 6481632-5 1984 Acridine Orange, an optical probe of H+ accumulation, was taken up preferentially by these parietal cells, exhibiting orange fluorescence within the cells on the third day of culture, in response to stimulation with gastrin, histamine and carbachol. Acridine Orange 0-15 gastrin Rattus norvegicus 216-223 2858486-5 1985 In the presence of ATP, the ATPase was capable of acidification as assessed by quenching of acridine orange. Acridine Orange 92-107 dynein axonemal heavy chain 8 Homo sapiens 28-34 3874738-4 1985 The decrease found in C1q binding material in the patient"s serum was paralleled by a decrease in serum immune complexes containing acridine orange binding material, possibly representing ds-DNA. Acridine Orange 132-147 complement C1q A chain Homo sapiens 22-25 6212085-0 1982 [Acridine orange inhibition of the ATPase activity of myosin and its fragments]. Acridine Orange 1-16 myosin heavy chain 14 Homo sapiens 54-60 6234306-9 1984 Measurements with acridine orange and oxonol V indicate that the reconstituted ATPase retains its transport activity, generating both delta pH and delta psi during the hydrolysis of MgATP. Acridine Orange 18-33 dynein axonemal heavy chain 8 Homo sapiens 79-85 6421932-8 1984 By using the metachromatic fluorescence stain acridine orange, we found that AdR blocked the increased synthesis of RNA that characterizes the entry into the G1 phase of the cell cycle from the G0, resting state. Acridine Orange 46-61 aldo-keto reductase family 1 member B Homo sapiens 77-80 6282137-1 1982 The weak base acridine orange (AO) has been shown to be accumulated by the insulin-containing secretory granules of cultured beta-cells in response to high glucose. Acridine Orange 14-29 insulin Homo sapiens 75-82 6282137-1 1982 The weak base acridine orange (AO) has been shown to be accumulated by the insulin-containing secretory granules of cultured beta-cells in response to high glucose. Acridine Orange 31-33 insulin Homo sapiens 75-82 6212085-1 1982 The cation dye acridine orange (AO) was shown to inhibit ATPase activity of myosin, DTNB-myosin and heavy meromyosin and not to influence that of EDTA-S-1 at low ionic force. Acridine Orange 15-30 myosin heavy chain 14 Homo sapiens 76-82 6212085-1 1982 The cation dye acridine orange (AO) was shown to inhibit ATPase activity of myosin, DTNB-myosin and heavy meromyosin and not to influence that of EDTA-S-1 at low ionic force. Acridine Orange 32-34 myosin heavy chain 14 Homo sapiens 76-82 6212085-3 1982 The allosteric influence of AO on myosin ATPase activity is discussed and dependence of this effect on charge distribution on the surface of the protein molecule is considered. Acridine Orange 28-30 myosin heavy chain 14 Homo sapiens 34-40 330821-9 1977 Treatment with visible light and acridine orange (photodynamic treatment) cause no significant degradation of DNA in the wild-type strain, a highly significant increase in the extent of DNA degradation in a polA mutant, and a decrease in the extent of degradation in the recA-type mutant. Acridine Orange 33-48 DNA polymerase I Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 207-211 330821-9 1977 Treatment with visible light and acridine orange (photodynamic treatment) cause no significant degradation of DNA in the wild-type strain, a highly significant increase in the extent of DNA degradation in a polA mutant, and a decrease in the extent of degradation in the recA-type mutant. Acridine Orange 33-48 recombinase RecA Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 271-275 138286-0 1976 On the mechanism of the acridine orange sensitized photodynamic inactivation of lysozyme. Acridine Orange 24-39 lysozyme Homo sapiens 80-88 326672-7 1977 The A25(R) phenotype was unstable and, as expected for plasmid-coded properties, acridine orange induced segregation of this phenotype. Acridine Orange 81-96 immunoglobulin kappa variable 1-22 (pseudogene) Homo sapiens 4-7 138286-3 1976 The photodynamic deactivation of lysozyme in presence of acridine orange is caused by a reaction between singlet oxygen formed via the dye triplet state and the protein. Acridine Orange 57-72 lysozyme Homo sapiens 33-41 6039958-0 1967 Photosensitivity of MS2 coliphage and its infective RNA after treatment with acridine orange. Acridine Orange 77-92 MS2 Homo sapiens 20-23 59662-2 1976 Stained with acridine-orange und 9-aminoacridine chloride-1-hydrate mycoplasmas appear as yellow (green) fluorescing homogeneous spots or discs under the fluorescence microscope, the exact appearance depending on the concentration of the fluorochrome and the absorption by nucleic acids. Acridine Orange 13-28 collagen type XIV alpha 1 chain Homo sapiens 29-32 5828-0 1976 On the mechanism of the acridine orange sensitized photodynamic inactivation of lysozyme. Acridine Orange 24-39 lysozyme Homo sapiens 80-88 5828-3 1976 The kinetics of the photodynamic desactivation of lysozyme in presence of acridine orange as the sensitizer have been investigated in detail varying oxygen, protein, dye concentration, ionic strength and pH value. Acridine Orange 74-89 lysozyme Homo sapiens 50-58 1166522-1 1975 Lymphocytes of human peripheral blood treated with PHA in short-living cultures can be divided into two subpopulations of cells according to their capacity to bind acridine orange. Acridine Orange 164-179 lamin B receptor Homo sapiens 51-54 1145760-3 1975 Additional extraction of f2 and f3 by 0.05 N HC1 (partial extraction) and by 0.25 N HC1 (more complete extraction) resulted in increased binding of acridine orange (1.9 and 2.5 of that of the control, respectively).9222 Acridine Orange 148-163 CYCS pseudogene 39 Homo sapiens 45-48 1145760-3 1975 Additional extraction of f2 and f3 by 0.05 N HC1 (partial extraction) and by 0.25 N HC1 (more complete extraction) resulted in increased binding of acridine orange (1.9 and 2.5 of that of the control, respectively).9222 Acridine Orange 148-163 CYCS pseudogene 39 Homo sapiens 84-87 5450756-2 1970 Change in the orientation of acridine orange in glycerinated fibers under the effects of ATP]. Acridine Orange 29-44 ATPase phospholipid transporting 8A2 Homo sapiens 89-93 33846805-3 2021 The present study including experiments of western blotting, acridine orange staining and transmission electron microscopy revealed that PRIMA-1met induced autophagy in CRC cells independently of p53 status. Acridine Orange 61-76 proline rich membrane anchor 1 Homo sapiens 137-144 33747176-8 2021 The results indicated that both sh-PRDX3 and miR-383 mimics promoted apoptosis and increased the level of mitochondrial ROS, whilst acridine orange staining revealed that sh-PRDX3 promoted autophagy in U87 cells compared with that in the control cells. Acridine Orange 132-147 peroxiredoxin 3 Homo sapiens 174-179 33929476-5 2021 Fluorescence staining at 12 mug mL-1 using Acridine Orange showed clear separability between the fluorescent intensities of the malaria parasites and that of the red blood cells (RBCs) and background. Acridine Orange 43-58 L1 cell adhesion molecule Mus musculus 32-36 33627540-9 2021 Acridine orange staining showed that ARG induced autophagy in PC-3M cells. Acridine Orange 0-15 ABL proto-oncogene 2, non-receptor tyrosine kinase Homo sapiens 37-40 33135957-12 2021 CRG protocol was validated in DAPI, acridine orange and Giemsa stain, and in 3 other laboratories. Acridine Orange 36-51 chromodomain helicase DNA binding protein 7 Homo sapiens 0-3 33886060-9 2021 Apoptotic effects of CB2 agonist were revealed with the increase of apoptotic cells in Acridine orange/Ethidium bromide staining, clear DNA fragmentation, pro-apoptotic genes and proteins upregulation (Caspase-3 and p53), and significant downregulation of anti-apoptotic Bcl-2. Acridine Orange 87-102 cannabinoid receptor 2 Homo sapiens 21-24 33906007-9 2021 This trend may be explained by enhanced acidic vesicular organelles, detected by acridine orange staining, determining a reduction of intracellular pH, that promotes new synthesis of TfR-1 degraded in a recycling pathway. Acridine Orange 81-96 transferrin receptor Homo sapiens 183-188 33135957-18 2021 PBMCs harvested with CRG protocol stained well in DAPI, acridine orange and Giemsa, and showed high scorable BN frequency in all laboratories. Acridine Orange 56-71 chromodomain helicase DNA binding protein 7 Homo sapiens 21-24 31223032-7 2019 In HCE-T cells, the amount of DNA fragments was statistically significantly increased in acridine orange-, ethidium bromide-, hydrogen peroxide-, 4-NQO- and MMS-treated cells but not in paraquat- or acrylamide-treated cells. Acridine Orange 89-104 RNA guanylyltransferase and 5'-phosphatase Homo sapiens 3-6 33277876-16 2020 The AO/EB staining assay showed that CHAF1A knockdown promotes apoptosis which is associated with downregulation of Bcl-2 and upregulation of Bax. Acridine Orange 4-6 chromatin assembly factor 1 subunit A Homo sapiens 37-43 32860827-5 2021 The additional stability of the rG4 structure was provided by the acridine orange derivative ligand C8, which stabilizes the pre-miR-92b rG4 structure, as denoted by an increase in more than 30 C of its melting temperature. Acridine Orange 66-81 G4 protein Rattus norvegicus 32-35 32314457-8 2020 Mechanistically, transient inhibition of FUCA1 promoted the formation of large acidic vacuoles, as revealed by staining with acridine orange, increased the ratio of LC3-B/LC3-A, and modified the expression of Beclin-1 and Atg12, which are autophagic markers. Acridine Orange 125-140 alpha-L-fucosidase 1 Homo sapiens 41-46 33456457-2 2020 In this study, we aimed to explore the brain functional correlates of the observation of human gait in PD patients with (FOG+) and without (FOG-) FOG and to investigate a possible relationship between AO-induced brain activation and gait performance. Acridine Orange 201-203 zinc finger protein, FOG family member 1 Homo sapiens 121-124 33456457-2 2020 In this study, we aimed to explore the brain functional correlates of the observation of human gait in PD patients with (FOG+) and without (FOG-) FOG and to investigate a possible relationship between AO-induced brain activation and gait performance. Acridine Orange 201-203 zinc finger protein, FOG family member 1 Homo sapiens 140-143 33456457-2 2020 In this study, we aimed to explore the brain functional correlates of the observation of human gait in PD patients with (FOG+) and without (FOG-) FOG and to investigate a possible relationship between AO-induced brain activation and gait performance. Acridine Orange 201-203 zinc finger protein, FOG family member 1 Homo sapiens 140-143 33016107-3 2020 The function of CAVIN2 was tested by Cell Counting Kit-8, colony formation, Transwell, flow cytometric analysis, acridine orange/ethidium bromide, chemosensitivity assay and xenograft assay. Acridine Orange 113-128 caveolae associated protein 2 Homo sapiens 16-22 32799888-8 2020 Acridine orange (AO) staining, as well as LysoSensor Green DND-189, cathepsin-B (CTSB), and cathepsin-D (CTSD) activities, showed that TMEM175 deficiency inhibited the hydrolytic function of lysosomes by affecting lysosomal pH. Acridine Orange 0-15 transmembrane protein 175 Rattus norvegicus 135-142 32339349-5 2020 To exhibit the morphology of the cells, the BM-MSCs were stained by Acridine Orange (AO) solution. Acridine Orange 68-83 hemoglobin subunit mu Homo sapiens 44-46 32726125-10 2020 Elevated miR-375 or reduced ATG2B decreased cell proliferation, acridine orange-positive cells and LC3-II/LC3-I ratio, and increased apoptosis rate in U2OS/DDP and MG63/DDP cells, as accompanied with lower IC50 value for DDP. Acridine Orange 64-79 microRNA 375 Homo sapiens 9-16 32726125-10 2020 Elevated miR-375 or reduced ATG2B decreased cell proliferation, acridine orange-positive cells and LC3-II/LC3-I ratio, and increased apoptosis rate in U2OS/DDP and MG63/DDP cells, as accompanied with lower IC50 value for DDP. Acridine Orange 64-79 autophagy related 2B Homo sapiens 28-33 32119860-7 2020 We demonstrated, that treatment of RBCs with both clustering agents caused an elevation in the percent of cells positively labeled by Annexin-V (RBCPS), and that this value was not dependent on the presence of calcium in the buffer: RBCs treated with AO in the presence of either EDTA, EGTA or calcium exhibited similar percentage of RBCPS. Acridine Orange 251-253 annexin A5 Homo sapiens 134-143 32337914-0 2020 Changes in the Level of DNA Fragmentation in Sperm Cells detected by Acridine Orange Test in Men with Sub/infertility Treated with Nutritional Supplement PAPA. Acridine Orange 69-84 pappalysin 1 Homo sapiens 154-158 32337914-4 2020 AIM: To study the effect of PAPA nutritional supplement on the levels of DNA fragmentation of sperm cells tested with acridine orange test (single stranded DNA against double stranded DNA) in men with sub/infertility. Acridine Orange 118-133 pappalysin 1 Homo sapiens 28-32 30987839-4 2019 To overcome these limitations, we developed a novel anti-vWF aptamer, called DTRI-031, that selectively binds and inhibits vWF-mediated platelet adhesion and arterial thrombosis while enabling rapid reversal of this antiplatelet activity by an antidote oligonucleotide (AO). Acridine Orange 270-272 Von Willebrand factor Mus musculus 57-60 31786875-8 2019 The AO/EB staining showed that Ginsenoside (Rg1) inhibits the viability of cancer cells via induction of apoptotic cell death which was correlated with increase in Bax and decrease in Bcl-2 levels. Acridine Orange 4-6 protein phosphatase 1 regulatory subunit 3A Homo sapiens 44-47 31301466-4 2019 Herein, we have studied two quadruplex-forming DNA sequences derived from the nucleolin-targeted aptamer AS1411 as supramolecular carriers for the cancer-selective delivery of acridine orange derivatives (C3, C5 and C8) in cervical cancer cells. Acridine Orange 176-191 nucleolin Mus musculus 78-87 30987839-4 2019 To overcome these limitations, we developed a novel anti-vWF aptamer, called DTRI-031, that selectively binds and inhibits vWF-mediated platelet adhesion and arterial thrombosis while enabling rapid reversal of this antiplatelet activity by an antidote oligonucleotide (AO). Acridine Orange 270-272 Von Willebrand factor Mus musculus 123-126 31135122-13 2019 Acridine orange, Monodansylcadaverine staining and western blotting analysis showed that PKM2 downregulation markedly increased autophagic cell death. Acridine Orange 0-15 pyruvate kinase M1/2 Homo sapiens 89-93 30179785-7 2019 The results showed that AO could be completely removed after 90 min electrolysis under the optimal condition: initial AO concentration was 30 mg L-1, current density was 50 mA cm-2, and the initial pH value was 5.0. Acridine Orange 24-26 L1 cell adhesion molecule Homo sapiens 145-148 30179785-7 2019 The results showed that AO could be completely removed after 90 min electrolysis under the optimal condition: initial AO concentration was 30 mg L-1, current density was 50 mA cm-2, and the initial pH value was 5.0. Acridine Orange 118-120 L1 cell adhesion molecule Homo sapiens 145-148 30165239-5 2019 AO is being used to enhance the ability of OCT, fluorescence imaging, and reflectance imaging. Acridine Orange 0-2 plexin A2 Homo sapiens 43-46 30538458-7 2018 Lysosomal membrane integrity, evaluated by acridine orange (AO) staining, was impaired by 40 microM SMA-tDodSNO (P<0.05 vs control) and when combined with SMA-Dox, this effect was significantly potentiated (P<0.001 vs SMA-Dox). Acridine Orange 43-58 immunoglobulin mu binding protein 2 Mus musculus 100-103 30538458-7 2018 Lysosomal membrane integrity, evaluated by acridine orange (AO) staining, was impaired by 40 microM SMA-tDodSNO (P<0.05 vs control) and when combined with SMA-Dox, this effect was significantly potentiated (P<0.001 vs SMA-Dox). Acridine Orange 60-62 immunoglobulin mu binding protein 2 Mus musculus 100-103 29864936-8 2018 An increased population of apoptotic cells was detected upon NEAT1 depletion by acridine orange/ethidium bromide staining compared to control cells. Acridine Orange 80-95 nuclear paraspeckle assembly transcript 1 Homo sapiens 61-66 29395359-4 2018 Acridine orange staining images showed that U1 snRNA overexpression not only activated autophagy pathway, but also led to the autophagic-lysosomal system dysfunction in cells. Acridine Orange 0-15 RNA, U1 small nuclear 1 Homo sapiens 44-52 29916529-6 2018 The upregulation of WIF-1-induced autophagy in NSCLC cells was detected by transmission electron microscopy, acridine orange staining, punctate GFP-LC3 and immunoblotting-based LC3 flux assay. Acridine Orange 109-124 WNT inhibitory factor 1 Homo sapiens 20-25 28634860-4 2018 Acridine orange (AO) and caspase 3 staining showed an increased cell death in Hsp70 and Hsc70 mutant eyes. Acridine Orange 0-15 Heat shock protein cognate 4 Drosophila melanogaster 78-83 29388695-10 2018 Acridine orange staining showed RANKL elevated autophagy activity in these cells. Acridine Orange 0-15 tumor necrosis factor (ligand) superfamily, member 11 Mus musculus 32-37 28634860-4 2018 Acridine orange (AO) and caspase 3 staining showed an increased cell death in Hsp70 and Hsc70 mutant eyes. Acridine Orange 0-15 Heat shock protein cognate 1 Drosophila melanogaster 88-93 28634860-4 2018 Acridine orange (AO) and caspase 3 staining showed an increased cell death in Hsp70 and Hsc70 mutant eyes. Acridine Orange 17-19 Heat shock protein cognate 4 Drosophila melanogaster 78-83 28634860-4 2018 Acridine orange (AO) and caspase 3 staining showed an increased cell death in Hsp70 and Hsc70 mutant eyes. Acridine Orange 17-19 Heat shock protein cognate 1 Drosophila melanogaster 88-93 29303982-5 2018 In this study, the cytotoxic effects of the curcumin analog DK1 was investigated in both U-2OS and MG-63 osteosarcoma cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell death was microscopically examined via acridine orange/propidium iodide (AO/PI) double staining. Acridine Orange 256-271 immunoglobulin heavy diversity 5-12 Homo sapiens 60-63 28731187-7 2017 Acridine orange/ethidium bromide staining indicated significant SHP-induced apoptosis. Acridine Orange 0-15 nuclear receptor subfamily 0 group B member 2 Homo sapiens 64-67 29129745-0 2018 Fluorescent light-up acridine orange derivatives bind and stabilize KRAS-22RT G-quadruplex. Acridine Orange 21-36 KRAS proto-oncogene, GTPase Homo sapiens 68-72 29138833-10 2018 Acridine orange (AO) staining and expression of LC3B II/I and p62 confirmed the inhibition of autophagy by TGF-beta1, and the recovery of TGF-beta1-mediated inhibition of autophagy by miR-16 mimics. Acridine Orange 0-15 transforming growth factor beta 1 Homo sapiens 138-147 29138833-10 2018 Acridine orange (AO) staining and expression of LC3B II/I and p62 confirmed the inhibition of autophagy by TGF-beta1, and the recovery of TGF-beta1-mediated inhibition of autophagy by miR-16 mimics. Acridine Orange 17-19 transforming growth factor beta 1 Homo sapiens 138-147 29675159-1 2018 The density functional theory method is used to elucidate the elementary steps of Ni(ii)-catalyzed C(sp2)-H iodination with I2 and substrates bearing N,N"-bidentate directing centers, amide-oxazoline (AO) and 8-aminoquinoline (AQ). Acridine Orange 201-203 Sp2 transcription factor Homo sapiens 99-104 29167462-2 2017 Previous studies show that the pore in KCNQ1 channels opens when the VSD activates to both intermediate and fully activated states, resulting in the intermediate open (IO) and activated open (AO) states, respectively. Acridine Orange 192-194 potassium voltage-gated channel subfamily Q member 1 Homo sapiens 39-44 29167462-4 2017 Here we show that inactivation of KCNQ1 channels derives from the different mechanisms of the VSD-pore coupling that lead to the IO and AO states, respectively. Acridine Orange 136-138 potassium voltage-gated channel subfamily Q member 1 Homo sapiens 34-39 28916337-8 2017 Acridine orange staining indicated that Ang II induced accumulation of acidic vacuoles. Acridine Orange 0-15 angiotensinogen Homo sapiens 40-46 28724188-3 2017 The mechanism of cytotoxicity of 3a involved caspase-2-dependent apoptotic pathway with characteristic apoptotic morphological alterations as observed in acridine orange/ethidium bromide and Hoechst staining. Acridine Orange 154-169 caspase 2 Homo sapiens 45-54 29061014-6 2017 The apoptosis of EpCAM-positive tumor cells was detected by acridine orange/ethidium bromide (AO/EB) double staining. Acridine Orange 60-75 epithelial cell adhesion molecule Homo sapiens 17-22 28694750-5 2017 Based on cytotoxicity data C1, C2 and C3 were selected for further in vitro mechanistic studies, namely apoptotic studies (Acridine orange/Ethidium bromide (AO/EB) and Annexin V), cell cycle analysis using propidium iodide (PI) stain and in vivo anticancer efficacy in 1,2-dimethyl hydrazine (DMH) induced colorectal carcinoma in Wistar rats. Acridine Orange 123-138 complement C2 Rattus norvegicus 27-40 28693266-5 2017 Using the Cell Counting Kit-8 assay, western blot analysis and acridine orange/ethidium bromide staining, downregulation of GluA2 was revealed to significantly inhibit the proliferation and significantly promote the apoptosis of A549 cells. Acridine Orange 63-78 glutamate ionotropic receptor AMPA type subunit 2 Homo sapiens 124-129 28553099-13 2017 The crude odds ratio of the COPD-Q for AO was 5.8. Acridine Orange 39-41 COPD Homo sapiens 28-32 29142391-5 2017 Morphological changes of ACS treated SCC-25 cells was evaluated by acridine orange/ethidium bromide (AO/EB) dual staining. Acridine Orange 67-82 serpin family B member 3 Homo sapiens 37-40 28648844-8 2017 Within the germline of etr-1(RNAi) hermaphrodite animals, we observe a decrease in average oocyte size and an increase in the number of germline apoptotic cell corpses as evident by an increased number of CED-1::GFP and acridine orange positive apoptotic germ cells. Acridine Orange 220-235 RRM domain-containing protein Caenorhabditis elegans 23-28 28218663-6 2017 Immunofluorescence of p62 and acridine orange staining revealed that AGEs significantly increased the expression of p62 and the accumulation of autophagic vacuoles, respectively. Acridine Orange 30-45 nucleoporin 62 Homo sapiens 116-119 27459118-8 2016 Acridine orange staining was absent in INS-1 (hypoxoside) and MIA PaCa-2 (hyperoside) treated cells, whereas LC3B expression was not significantly increased. Acridine Orange 0-15 forkhead box M1 Homo sapiens 39-44 28038323-7 2017 Moreover, DAPI and acridine orange/ethidium bromide staining studies indicated that compounds 11c and 11f can induce apoptosis in PC-3 cells. Acridine Orange 19-34 chromobox 8 Homo sapiens 130-134 28182147-6 2017 Notably, SiNPs impaired the lysosomal function through damaging lysosomal ultrastructures, increasing membrane permeability, and downregulating the expression of lysosomal proteases, cathepsin B, as evidenced by transmission electron microscopy, acridine orange staining, quantitative reverse transcription-polymerase chain reaction, and Western blot assays. Acridine Orange 246-261 cathepsin B Homo sapiens 183-194 28056508-5 2017 The biological relevance of ATP6V1A in mediating the neuroprotective effects of FK506 was validated by analyzing FK506 activity with respect to autophagy via acridine orange staining and transcription factor EB (TFEB) translocation assay. Acridine Orange 158-173 ATPase H+ transporting V1 subunit A Homo sapiens 28-35 27875278-6 2016 Results obtained with Acridine Orange, considering R/GFIR, correlated with the conversion of the unlipidated form of LC3 (LC3-I) into the lipidated form (LC3-II), SQSTM1 degradation and GFP-LC3 puncta formation, thus validating this assay to be used as an initial and quantitative method for evaluating the late step of autophagy in individual cells, complementing other methods. Acridine Orange 22-37 microtubule associated protein 1 light chain 3 alpha Homo sapiens 117-120 27875278-6 2016 Results obtained with Acridine Orange, considering R/GFIR, correlated with the conversion of the unlipidated form of LC3 (LC3-I) into the lipidated form (LC3-II), SQSTM1 degradation and GFP-LC3 puncta formation, thus validating this assay to be used as an initial and quantitative method for evaluating the late step of autophagy in individual cells, complementing other methods. Acridine Orange 22-37 microtubule associated protein 1 light chain 3 alpha Homo sapiens 122-127 27875278-6 2016 Results obtained with Acridine Orange, considering R/GFIR, correlated with the conversion of the unlipidated form of LC3 (LC3-I) into the lipidated form (LC3-II), SQSTM1 degradation and GFP-LC3 puncta formation, thus validating this assay to be used as an initial and quantitative method for evaluating the late step of autophagy in individual cells, complementing other methods. Acridine Orange 22-37 microtubule associated protein 1 light chain 3 alpha Homo sapiens 122-125 27875278-6 2016 Results obtained with Acridine Orange, considering R/GFIR, correlated with the conversion of the unlipidated form of LC3 (LC3-I) into the lipidated form (LC3-II), SQSTM1 degradation and GFP-LC3 puncta formation, thus validating this assay to be used as an initial and quantitative method for evaluating the late step of autophagy in individual cells, complementing other methods. Acridine Orange 22-37 sequestosome 1 Homo sapiens 163-169 27875278-6 2016 Results obtained with Acridine Orange, considering R/GFIR, correlated with the conversion of the unlipidated form of LC3 (LC3-I) into the lipidated form (LC3-II), SQSTM1 degradation and GFP-LC3 puncta formation, thus validating this assay to be used as an initial and quantitative method for evaluating the late step of autophagy in individual cells, complementing other methods. Acridine Orange 22-37 microtubule associated protein 1 light chain 3 alpha Homo sapiens 122-125 27459118-8 2016 Acridine orange staining was absent in INS-1 (hypoxoside) and MIA PaCa-2 (hyperoside) treated cells, whereas LC3B expression was not significantly increased. Acridine Orange 0-15 MIA SH3 domain containing Homo sapiens 62-65 25809873-6 2015 Results of Nedd4 expression on lysosomal membrane permeabilization and autophagy were further investigated using acridine orange (AO) staining, immunofluorescence and western blot analysis. Acridine Orange 113-128 NEDD4 E3 ubiquitin protein ligase Homo sapiens 11-16 26636353-3 2015 A wide range of planar aromatic compounds bind NQO2, and we have identified three DNA-intercalating agents [ethidium bromide, acridine orange (AO), and doxorubicin] as novel nanomolar inhibitors of NQO2. Acridine Orange 126-141 N-ribosyldihydronicotinamide:quinone reductase 2 Homo sapiens 47-51 26636353-3 2015 A wide range of planar aromatic compounds bind NQO2, and we have identified three DNA-intercalating agents [ethidium bromide, acridine orange (AO), and doxorubicin] as novel nanomolar inhibitors of NQO2. Acridine Orange 126-141 N-ribosyldihydronicotinamide:quinone reductase 2 Homo sapiens 198-202 26636353-3 2015 A wide range of planar aromatic compounds bind NQO2, and we have identified three DNA-intercalating agents [ethidium bromide, acridine orange (AO), and doxorubicin] as novel nanomolar inhibitors of NQO2. Acridine Orange 143-145 N-ribosyldihydronicotinamide:quinone reductase 2 Homo sapiens 198-202 26636353-4 2015 Ethidium and AO, which carry a positive charge in their aromatic ring systems, bound reduced NQO2 with an affinity 50-fold higher than that of oxidized NQO2, while doxorubicin bound only oxidized NQO2. Acridine Orange 13-15 N-ribosyldihydronicotinamide:quinone reductase 2 Homo sapiens 93-97 26636353-4 2015 Ethidium and AO, which carry a positive charge in their aromatic ring systems, bound reduced NQO2 with an affinity 50-fold higher than that of oxidized NQO2, while doxorubicin bound only oxidized NQO2. Acridine Orange 13-15 N-ribosyldihydronicotinamide:quinone reductase 2 Homo sapiens 152-156 26636353-4 2015 Ethidium and AO, which carry a positive charge in their aromatic ring systems, bound reduced NQO2 with an affinity 50-fold higher than that of oxidized NQO2, while doxorubicin bound only oxidized NQO2. Acridine Orange 13-15 N-ribosyldihydronicotinamide:quinone reductase 2 Homo sapiens 152-156 26636353-7 2015 In reduced NQO2, ethidium and AO occupied a more peripheral position in the active site, allowing several water molecules to interact with the polar end of the negatively charged isoalloxazine ring. Acridine Orange 30-32 N-ribosyldihydronicotinamide:quinone reductase 2 Homo sapiens 11-15 26636353-8 2015 We also showed that AO inhibited NQO2 at a nontoxic concentration in cells while ethidium was less effective at inhibiting NQO2 in cells. Acridine Orange 20-22 N-ribosyldihydronicotinamide:quinone reductase 2 Homo sapiens 33-37 26420839-9 2015 Patient hTEC produced and secreted elevated amounts of the pro-inflammatory cytokine IL8, which was highly correlated with the acridine orange staining. Acridine Orange 127-142 C-X-C motif chemokine ligand 8 Homo sapiens 85-88 26239968-7 2015 Moreover, examination of microtubule-associated protein 1A/1B-light chain 3 (LC3I)/LC3II conversion and acridine orange staining for autophagic vesicles, combined with flow cytometry, showed that treatment with AD1 induced the autophagic pathway in U87MG and U373MG cells. Acridine Orange 104-119 amyloid beta precursor protein Homo sapiens 211-214 26990000-4 2016 Further studies revealed that selected hybrid 5 could induce apoptosis in Hep-G2 cells through the investigation of acridine orange/ethidium bromide, Hoechst 33258 fluorescence stainings, and annexin V/propidium iodide assay. Acridine Orange 116-131 DNL-type zinc finger Homo sapiens 74-77 27022281-9 2016 We experimentally verified that one of these novel agents, prenylamine, induced HCC cell apoptosis using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, an acridine orange/ethidium bromide stain, and electron microscopy. Acridine Orange 170-185 HCC Homo sapiens 80-83 26016416-3 2016 The Sm(III)-(Trp)3 complex was stabilized by intercalation into the DNA with binding constants: K(O)25 C = 7.14 x 10(5) L mol(-1) and K(O) 37 C = 5.28 x 10(4) L mol(-1), and it could displace the AO dye from the AO-DNA complex in a competitive reaction. Acridine Orange 200-202 tRNA-Pro (anticodon TGG) 3-1 Homo sapiens 4-18 26174122-5 2015 With acridine orange (AO) staining and flow analysis, we found 7-HDNF induced the formation of intracellular vacuoles and the augmentation of acidic vesicular organelles (AVO). Acridine Orange 5-20 neurotrophin 3 Homo sapiens 65-69 26174122-5 2015 With acridine orange (AO) staining and flow analysis, we found 7-HDNF induced the formation of intracellular vacuoles and the augmentation of acidic vesicular organelles (AVO). Acridine Orange 22-24 neurotrophin 3 Homo sapiens 65-69 24596333-6 2015 Acridine orange staining results revealed that TBBPA exposure caused cardiomyocyte apoptosis and induced the expression of three proapoptotic genes: P53, Bax, and Caspase9. Acridine Orange 0-15 tumor protein p53 Danio rerio 149-152 24596333-6 2015 Acridine orange staining results revealed that TBBPA exposure caused cardiomyocyte apoptosis and induced the expression of three proapoptotic genes: P53, Bax, and Caspase9. Acridine Orange 0-15 caspase 9, apoptosis-related cysteine peptidase Danio rerio 163-171 25809873-6 2015 Results of Nedd4 expression on lysosomal membrane permeabilization and autophagy were further investigated using acridine orange (AO) staining, immunofluorescence and western blot analysis. Acridine Orange 130-132 NEDD4 E3 ubiquitin protein ligase Homo sapiens 11-16 25683690-2 2015 We report here time-resolved fluorescence studies for acridine orange complexes with cucurbit[7]uril and cucurbit[8]uril in the presence of human serum albumin as a model system. Acridine Orange 54-69 albumin Homo sapiens 146-159 25683690-3 2015 A detailed characterization of the fluorescence lifetime and anisotropy properties of the different acridine orange complexes with cucurbit[n]urils and human serum albumin was performed. Acridine Orange 100-115 albumin Homo sapiens 158-171 25884412-3 2015 However, peptides prepared from Mf conjugated with alginate oligosaccharide (AO; 19 mug/mg protein) (dMSA) through the Maillard reaction in the presence of sorbitol significantly reduced the secretion of the pro-inflammatory mediators nitric oxide, tumor necrosis factor (TNF)-alpha and interleukin (IL)-6, as well as mRNA expression of TNF-alpha, IL-6, inducible nitric oxide synthase and cyclooxygenase-2. Acridine Orange 77-79 interleukin 6 Mus musculus 348-352 25884412-3 2015 However, peptides prepared from Mf conjugated with alginate oligosaccharide (AO; 19 mug/mg protein) (dMSA) through the Maillard reaction in the presence of sorbitol significantly reduced the secretion of the pro-inflammatory mediators nitric oxide, tumor necrosis factor (TNF)-alpha and interleukin (IL)-6, as well as mRNA expression of TNF-alpha, IL-6, inducible nitric oxide synthase and cyclooxygenase-2. Acridine Orange 77-79 tumor necrosis factor Mus musculus 249-282 26146506-4 2015 The results showed that the cells morphologies changed significantly; ACSs induced cell death in B16 and 4T1 cells based on acridine orange/ethidium bromide double fluorescence staining, with the number and degree of apoptotic tumor cells increasing as ACS concentration increased. Acridine Orange 124-139 acyl-CoA synthetase short-chain family member 2 Mus musculus 70-73 25884412-3 2015 However, peptides prepared from Mf conjugated with alginate oligosaccharide (AO; 19 mug/mg protein) (dMSA) through the Maillard reaction in the presence of sorbitol significantly reduced the secretion of the pro-inflammatory mediators nitric oxide, tumor necrosis factor (TNF)-alpha and interleukin (IL)-6, as well as mRNA expression of TNF-alpha, IL-6, inducible nitric oxide synthase and cyclooxygenase-2. Acridine Orange 77-79 interleukin 6 Mus musculus 287-305 25884412-3 2015 However, peptides prepared from Mf conjugated with alginate oligosaccharide (AO; 19 mug/mg protein) (dMSA) through the Maillard reaction in the presence of sorbitol significantly reduced the secretion of the pro-inflammatory mediators nitric oxide, tumor necrosis factor (TNF)-alpha and interleukin (IL)-6, as well as mRNA expression of TNF-alpha, IL-6, inducible nitric oxide synthase and cyclooxygenase-2. Acridine Orange 77-79 tumor necrosis factor Mus musculus 337-346 25884412-3 2015 However, peptides prepared from Mf conjugated with alginate oligosaccharide (AO; 19 mug/mg protein) (dMSA) through the Maillard reaction in the presence of sorbitol significantly reduced the secretion of the pro-inflammatory mediators nitric oxide, tumor necrosis factor (TNF)-alpha and interleukin (IL)-6, as well as mRNA expression of TNF-alpha, IL-6, inducible nitric oxide synthase and cyclooxygenase-2. Acridine Orange 77-79 prostaglandin-endoperoxide synthase 2 Mus musculus 390-406 25063800-4 2014 We hypothesize that acute ozone (AO) exposure during postnatal airway development disrupts SP/NK-1R/Nur77 pathway expression and that these changes correlate with increased ozone-induced cell death. Acridine Orange 33-35 tachykinin precursor 1 Homo sapiens 91-93 25073513-6 2014 Staining cells with acridine orange and ethidium bromide, the PC-3 control cells showed largely non-fragmented intact nucleoid. Acridine Orange 20-35 chromobox 8 Homo sapiens 62-66 25275102-6 2014 It relies on the use of acridine orange (AO), a standard dye for pHL. Acridine Orange 24-39 BCR activator of RhoGEF and GTPase Homo sapiens 65-68 25275102-6 2014 It relies on the use of acridine orange (AO), a standard dye for pHL. Acridine Orange 41-43 BCR activator of RhoGEF and GTPase Homo sapiens 65-68 25063800-4 2014 We hypothesize that acute ozone (AO) exposure during postnatal airway development disrupts SP/NK-1R/Nur77 pathway expression and that these changes correlate with increased ozone-induced cell death. Acridine Orange 33-35 tachykinin receptor 1 Homo sapiens 94-99 25063800-4 2014 We hypothesize that acute ozone (AO) exposure during postnatal airway development disrupts SP/NK-1R/Nur77 pathway expression and that these changes correlate with increased ozone-induced cell death. Acridine Orange 33-35 nuclear receptor subfamily 4 group A member 1 Homo sapiens 100-105 24889814-5 2014 The formation of autophagic vacuoles and acidic vesicular organelles (AVOs) was observed in the ANK-199-treated CAR cells by monodansylcadaverine (MDC) and acridine orange (AO) staining, suggesting that ANK-199 is able to induce autophagic cell death in CAR cells. Acridine Orange 173-175 ankyrin 1 Homo sapiens 96-99 24698466-6 2014 Transcardiac cTnT release (DeltacTnT [CS-Ao]) represented the difference between CS and Ao-cTnT levels. Acridine Orange 41-43 troponin T2, cardiac type Homo sapiens 13-17 24989276-3 2014 Morphological changes of THP-1 cells treated with brucine was detected by acridine orange/ethidium bromide (AO/EB)double staining. Acridine Orange 74-89 GLI family zinc finger 2 Homo sapiens 25-30 24698466-6 2014 Transcardiac cTnT release (DeltacTnT [CS-Ao]) represented the difference between CS and Ao-cTnT levels. Acridine Orange 41-43 troponin T2, cardiac type Homo sapiens 32-36 24698466-10 2014 In patients with available cTnT release, DeltacTnT (CS-Ao) levels were significantly higher in LGE-positive patients than those in LGE-negative patients (4.3 [2.2-5.5] vs 1.5 [0.9-2.6] ng/L; p = 0.001). Acridine Orange 55-57 troponin T2, cardiac type Homo sapiens 27-31 24451478-6 2014 RESULTS: We found that selective inhibition of PFKFB3 with either siRNA transfection or 3PO in HCT-116 colon adenocarcinoma cells caused a marked decrease in glucose uptake simultaneously with an increase in autophagy based on LC3-II and p62 protein expression, acridine orange fluorescence of acidic vacuoles and electron microscopic detection of autophagosomes. Acridine Orange 262-277 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 Homo sapiens 47-53 24055436-0 2014 Real-time imaging of exocytotic mucin release and swelling in Calu-3 cells using acridine orange. Acridine Orange 81-96 LOC100508689 Homo sapiens 32-37 24055436-3 2014 In this report we present original spectroscopic properties of acridine orange (AO) which could be utilized to study granule release and mucin swelling with various advanced fluorescence imaging approaches. Acridine Orange 63-78 LOC100508689 Homo sapiens 137-142 24055436-3 2014 In this report we present original spectroscopic properties of acridine orange (AO) which could be utilized to study granule release and mucin swelling with various advanced fluorescence imaging approaches. Acridine Orange 80-82 LOC100508689 Homo sapiens 137-142 24055436-11 2014 In summary, we showed that AO-staining could be utilized for real-time TIRF imaging of mucin granule exocytosis and mucin swelling with high sensitivity and temporal resolution. Acridine Orange 27-29 LOC100508689 Homo sapiens 87-92 24055436-11 2014 In summary, we showed that AO-staining could be utilized for real-time TIRF imaging of mucin granule exocytosis and mucin swelling with high sensitivity and temporal resolution. Acridine Orange 27-29 LOC100508689 Homo sapiens 116-121 24183951-6 2014 Based on the in vitro cytotoxic assays and morphological assessment studies using fluorescence microscopic study (acridine orange and ethidium bromide double staining) and mitochondrial potential measurements, it was found that ice 2 and 3 possess good antiproliferative effect via mitochondrial mediated apoptosis in human lung and breast cancer cells. Acridine Orange 114-129 interactor of little elongation complex ELL subunit 2 Homo sapiens 228-239 24553109-6 2014 Furthermore, Ad-hTERT-E1a-HN was shown to induce the apoptosis pathway via acridine orange and ethidium bromide staining (AO/EB staining), increase reactive oxygen species (ROS), reduce mitochondrial membrane potential and release cytochrome c. Acridine Orange 75-90 telomerase reverse transcriptase Homo sapiens 16-21 24321340-3 2014 Here, we offered convincing evidence that CA-4 induced autophagy in various cancer cells, which was demonstrated by acridine orange staining of intracellular acidic vesicles, the degradation of p62, the conversion of LC3-I to LC3-II and GFP-LC3 punctate fluorescence. Acridine Orange 116-131 carbonic anhydrase 4 Homo sapiens 42-46 24451478-7 2014 The induction of autophagy caused by PFKFB3 inhibition required an increase in reactive oxygen species since N-acetyl-cysteine blocked both the conversion of LC3-I to LC3-II and the increase in acridine orange fluorescence in acidic vesicles after exposure of HCT-116 cells to 3PO. Acridine Orange 194-209 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 Homo sapiens 37-43 23545415-4 2013 In MDA-MB-231 and HCT116 cells, expressing mutant or wild type p53, respectively, autophagy occurred following exposure to PRIMA-1, as shown by acridine orange staining, anti-LC3 immunofluorescence and immunoblots, as well as by electron microscopy. Acridine Orange 144-159 tumor protein p53 Homo sapiens 63-66 24238063-5 2013 Treatment with the mTOR inhibitor, rapamycin, increased LC3-II and the content of both APs detected by Cyto-ID Green staining and autophagolysosomes (APLs) measured by acridine orange staining and colocalization of LC3 and Lamp1. Acridine Orange 168-183 mechanistic target of rapamycin kinase Mus musculus 19-23 23545415-4 2013 In MDA-MB-231 and HCT116 cells, expressing mutant or wild type p53, respectively, autophagy occurred following exposure to PRIMA-1, as shown by acridine orange staining, anti-LC3 immunofluorescence and immunoblots, as well as by electron microscopy. Acridine Orange 144-159 proline rich membrane anchor 1 Homo sapiens 123-130 23988451-10 2013 In contrast, autophagy, assessed using acridine orange staining, was induced with SGI-1776 treatment in both cell lines (U266, 25%-70%; MM.1S, 8%-52%) and CD138(+) cells (19%-21%). Acridine Orange 39-54 chromogranin B Homo sapiens 82-85 23128449-7 2013 Knockdown of Nrf2 also led to a significant increase of autophagic vacuoles and acidic vesicular organelles (AVOs), revealed by trans-mission electron microscopy (TEM) and acridine orange (AO) staining using flow cytometry. Acridine Orange 196-223 NFE2 like bZIP transcription factor 2 Homo sapiens 25-29 23196876-9 2013 The involvement of autophagy in endogenous and AUY922-induced KIT protein turnover was further confirmed by the colocalization of KIT with MAP1LC3B-, acridine orange- or SQSTM1-labeled autophagosome, and by the accumulation of KIT in GIST cells by silencing either BECN1 or ATG5 to disrupt autophagosome activity. Acridine Orange 150-165 KIT proto-oncogene, receptor tyrosine kinase Homo sapiens 130-133 23196876-9 2013 The involvement of autophagy in endogenous and AUY922-induced KIT protein turnover was further confirmed by the colocalization of KIT with MAP1LC3B-, acridine orange- or SQSTM1-labeled autophagosome, and by the accumulation of KIT in GIST cells by silencing either BECN1 or ATG5 to disrupt autophagosome activity. Acridine Orange 150-165 KIT proto-oncogene, receptor tyrosine kinase Homo sapiens 130-133 23128449-7 2013 Knockdown of Nrf2 also led to a significant increase of autophagic vacuoles and acidic vesicular organelles (AVOs), revealed by trans-mission electron microscopy (TEM) and acridine orange (AO) staining using flow cytometry. Acridine Orange 225-227 NFE2 like bZIP transcription factor 2 Homo sapiens 25-29 23128449-9 2013 Furthermore, after the treatment with TMZ (100 microM) for 3 days, the U251-Si-Nrf2 transfected cells showed less viability rate by cell counting kit-8 (CCK-8) assay and the levels of autophagy increased obviously through analysis of western blot and AO staining using flow cytometry. Acridine Orange 287-289 NFE2 like bZIP transcription factor 2 Homo sapiens 91-95 22739068-10 2012 The red fluorescence induced by siSET in HN13 cells in the acridine orange assay suggests SET-dependent prevention of AVOs acidification. Acridine Orange 59-74 MT-RNR2 like 13 (pseudogene) Homo sapiens 41-45 22676643-6 2012 We demonstrated that acridine orange staining and protein expressions of LC-3 and beclin-1 were increased in p53-transfected cells. Acridine Orange 21-36 beclin 1 Homo sapiens 82-90 22676643-6 2012 We demonstrated that acridine orange staining and protein expressions of LC-3 and beclin-1 were increased in p53-transfected cells. Acridine Orange 21-36 tumor protein p53 Homo sapiens 109-112 22029424-1 2011 The surface active derivative of the organic dye Acridine Orange (N-10-dodecyl-acridine orange (DAO)) has been included in mixed Langmuir monolayers with stearic acid (SA). Acridine Orange 49-64 D-amino acid oxidase Homo sapiens 66-101 22265822-7 2012 Acridine orange fluorescence intensity suggested that ATP13A2 induced the expansion of acidic vesicles that become more alkaline upon external addition of spermidine. Acridine Orange 0-15 ATPase cation transporting 13A2 Homo sapiens 54-61 23028562-6 2012 In ectopic expression studies, NijA induced cell death, as evidenced by cell loss and acridine orange staining. Acridine Orange 86-101 Ninjurin A Drosophila melanogaster 31-35 22445551-4 2012 Acridine orange staining and flow cytometry analysis also showed that the curcin could induce apoptosis of mouse sarcoma-180 cells. Acridine Orange 0-15 ribosome-inactivating protein cucurmosin Jatropha curcas 74-80 21072519-5 2011 4-HPR induced a robust, sustained increase in LC3 II expression and enhanced formation of acridine orange-stained acidic vesicles that are markers of autophagy. Acridine Orange 90-105 haptoglobin-related protein Homo sapiens 2-5 21382103-7 2011 The amount of DNA fragmentation assessed by SCD was highly correlated (R=0.874, p<0.05) with results of acridine orange test (AOT), a traditional method of assessing DNA damage. Acridine Orange 107-122 stearoyl-CoA desaturase Bubalus bubalis 44-47 20687516-6 2010 The results demonstrate increased DNA laddering by micrococcal nuclease and an increased amount of DNA intercalation by acridine orange in PARG null-TS cells. Acridine Orange 120-135 poly(ADP-ribose) glycohydrolase Homo sapiens 139-143 21186394-4 2011 The strongly fluorescent acridine orange, a nuclear targeting agent, has been derivatised with 4-imidazolecarboxylate as a bidentate ligand and bombesin with an isonitrile group as a monodentate ligand. Acridine Orange 25-40 gastrin releasing peptide Homo sapiens 144-152 21093514-5 2011 Acridine orange/ethidium bromide staining, a wound-healing assay, and a flow cytometric analysis showed that TH1-5 induced necrosis with high-concentration treatment and induced apoptosis with low-concentration treatment. Acridine Orange 0-15 negative elongation factor complex member C/D Homo sapiens 109-114 20674670-6 2010 By using FoxO3a morpholino antisense oligonucleotides, we observed that FoxO3a loss-of-function led to neural developmental defects, including increased neural apoptosis as detected by acridine orange and terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling. Acridine Orange 185-200 forkhead box O3A Danio rerio 72-78 21042905-0 2011 Acridine orange-induced signal enhancement effect of tyrosinase-immobilized carbon-felt-based flow biosensor for highly sensitive detection of monophenolic compounds. Acridine Orange 0-15 tyrosinase Homo sapiens 53-63 21042905-1 2011 Tyrosinase (TYR: EC 1.14.18.1) was covalently modified onto the surface of a cyanuric chloride-activated carbon felt (CF) from the mixed buffer solution of TYR and acridine orange (AO). Acridine Orange 164-179 tyrosinase Homo sapiens 0-10 21042905-1 2011 Tyrosinase (TYR: EC 1.14.18.1) was covalently modified onto the surface of a cyanuric chloride-activated carbon felt (CF) from the mixed buffer solution of TYR and acridine orange (AO). Acridine Orange 181-183 tyrosinase Homo sapiens 0-10 21720516-3 2011 Standard ethidium bromide and acridine orange assays were used to detect apoptotic cells and indicated that a significantly larger percentage of the cells (approx 40%) were dead after 72 hours of exposure to f-SWCNTs-p53 as compared to the control cells, which were exposed to only p53 or f-SWCNTs, respectively. Acridine Orange 30-45 tumor protein p53 Homo sapiens 217-220 20384836-3 2010 We thus obtained images of the different fluorescence emissions of acridine orange bound to single or double stranded nucleic acids in human metaphase chromosomes before and after DNAse I or RNAse A treatment. Acridine Orange 67-82 ribonuclease A family member 1, pancreatic Homo sapiens 191-198 26069556-8 2010 CONCLUSIONS: The AO/PO ratio in this study was a useful radiological parameter for the assessment of patients with a cam-type impingement. Acridine Orange 17-19 calmodulin 3 Homo sapiens 117-120 20064577-4 2010 Treatment of PC12 cells with recombinant human IGF-1 significantly decreased apoptosis caused by MPP(+) as measured by acridine orange/ethidium bromide staining. Acridine Orange 119-134 insulin like growth factor 1 Homo sapiens 47-52 21067415-3 2010 The results of acridine orange staining indicated that MLL-2 caused apoptosis in MCF-7 cells. Acridine Orange 15-30 lysine methyltransferase 2D Homo sapiens 55-60 20017221-2 2009 METHODS: Morphological changes of Hep-2 cells were observed by acridine orange cytochemistry staining. Acridine Orange 63-78 DNL-type zinc finger Homo sapiens 34-37 19517998-2 2009 In this paper, interactions of SPS isolated from Klebsiella strains K20 and K51 with cationic dyes pinacyanol chloride (PCYN) and acridine orange (AO) were studied by absorbance and fluorescence measurements. Acridine Orange 130-145 keratin 20 Homo sapiens 68-71 20079104-2 2009 METHODS: Morphological changes of Hep-2 cells were observed by reserved microscopy and acridine orange cytochemistry staining. Acridine Orange 87-102 DNL-type zinc finger Homo sapiens 34-37 19414550-6 2009 At both 3 and 7 days postchallenge, the TLR2(-/-) mice had higher blood bacterial titers than the WT mice (P < 0.05), and typical bacteria were identified in the effusion from both ears of both mouse groups by acridine orange staining. Acridine Orange 213-228 toll-like receptor 2 Mus musculus 40-44 19322641-4 2009 Inositol hexasulfate (InsS(6)), a structural analog of InsP(6), was used to determine specificity of InsP(6)-induced apoptosis as measured by acridine orange/ethidium bromide, flow cytometry, and DNA degradation. Acridine Orange 142-157 inositol hexaphosphate kinase 1 Mus musculus 101-108 19517998-2 2009 In this paper, interactions of SPS isolated from Klebsiella strains K20 and K51 with cationic dyes pinacyanol chloride (PCYN) and acridine orange (AO) were studied by absorbance and fluorescence measurements. Acridine Orange 147-149 keratin 20 Homo sapiens 68-71 19257877-7 2009 Following acridine orange and ethidium bromide staining, treatment with 10, 12 and 14 mug mL-1 of ethanolic extracts caused typical apoptotic morphological changes in HepG2 cells. Acridine Orange 10-25 L1 cell adhesion molecule Mus musculus 90-94 18815872-4 2009 The use of acridine orange as fluorescent probe permitted to determine both CMC(1) and CMC(2). Acridine Orange 11-26 C-X9-C motif containing 1 Homo sapiens 76-82 18815872-4 2009 The use of acridine orange as fluorescent probe permitted to determine both CMC(1) and CMC(2). Acridine Orange 11-26 C-X9-C motif containing 2 Homo sapiens 87-93 18284246-1 2008 An efficient enantioselective synthesis of sn-2-aminooxy (AO) analogues of lysophosphatidic acid (LPA) that possess palmitoyl and oleoyl acyl chains is presented. Acridine Orange 58-60 solute carrier family 38 member 5 Homo sapiens 43-47 18959757-2 2008 In this study, we used microtubule-associated protein 1 light chain 3 processing and acridine orange staining to reveal that knockdown of integrin beta4 by its specific siRNA induced autophagic cell death in A549 lung cancer cells. Acridine Orange 85-100 integrin subunit beta 4 Homo sapiens 138-152 18810578-4 2008 During the acute phase of AMI, plasma levels of VWF: Ag were similar in FV, Ao, and Cs, and were higher than those of age-matched control. Acridine Orange 76-78 von Willebrand factor Homo sapiens 48-51 18814910-5 2009 In the presence of the photosensitising agents acridine orange (100 nM) or hypericin (10 nM), the sensitivity of light-induced TRPA1 activation was increased and extended towards the visible spectrum. Acridine Orange 47-62 transient receptor potential cation channel subfamily A member 1 Homo sapiens 127-132 25084402-1 2009 Tyrosinase (TYR, EC 1.14.18.1) was physically adsorbed onto a carbon felt (CF) together with acridine orange (AO). Acridine Orange 93-108 tyrosinase Homo sapiens 0-10 25084402-1 2009 Tyrosinase (TYR, EC 1.14.18.1) was physically adsorbed onto a carbon felt (CF) together with acridine orange (AO). Acridine Orange 93-108 tyrosinase Homo sapiens 12-15 25084402-1 2009 Tyrosinase (TYR, EC 1.14.18.1) was physically adsorbed onto a carbon felt (CF) together with acridine orange (AO). Acridine Orange 110-112 tyrosinase Homo sapiens 0-10 25084402-1 2009 Tyrosinase (TYR, EC 1.14.18.1) was physically adsorbed onto a carbon felt (CF) together with acridine orange (AO). Acridine Orange 110-112 tyrosinase Homo sapiens 12-15 25084402-2 2009 Coadsorption of AO was essential to prevent the denaturation of the TYR at the CF surface. Acridine Orange 16-18 tyrosinase Homo sapiens 68-71 18335304-2 2008 However, in the present work we discovered that CdS quantum dots sharply quenched the fluorescence of acridine orange (AO). Acridine Orange 102-117 CDP-diacylglycerol synthase 1 Homo sapiens 48-51 18335304-2 2008 However, in the present work we discovered that CdS quantum dots sharply quenched the fluorescence of acridine orange (AO). Acridine Orange 119-121 CDP-diacylglycerol synthase 1 Homo sapiens 48-51 17409468-8 2007 The acridine orange assay revealed a significant increase in sperm with abnormal DNA integrity profiles in the 1x CHOP group. Acridine Orange 4-19 DNA-damage inducible transcript 3 Rattus norvegicus 114-118 19081762-5 2008 Further studies showed that in 10-08-MG, U-251MG, U-87MG, and T98G cells PATZ1 siRNA significantly increased apoptosis in response to incubation with soluble FasL, as shown by a morphologic acridine orange/ethidium bromide apoptotic assay. Acridine Orange 190-205 POZ/BTB and AT hook containing zinc finger 1 Homo sapiens 73-78 19081762-5 2008 Further studies showed that in 10-08-MG, U-251MG, U-87MG, and T98G cells PATZ1 siRNA significantly increased apoptosis in response to incubation with soluble FasL, as shown by a morphologic acridine orange/ethidium bromide apoptotic assay. Acridine Orange 190-205 Fas ligand Homo sapiens 158-162 18068224-8 2008 An increased number of apoptotic cells in the eye and neural tissues were observed in MIF morphants by histological analysis and acridine orange staining. Acridine Orange 129-144 macrophage migration inhibitory factor Danio rerio 86-89 17685640-3 2007 The diffusion coefficient, D, of acridine orange in pure solvent was found to be 4 times smaller at the water/C18 interface (D = 0.022 x 10(-4) cm2/s) than in bulk water (D = 0.087 x 10(-4) cm2/s), in qualitative agreement with experiment. Acridine Orange 33-48 Bardet-Biedl syndrome 9 Homo sapiens 110-113 17363491-7 2007 ClC-3 overexpression increased the acidity of intracellular vesicles, as assessed by acridine orange staining, and enhanced resistance to the chemotherapeutic drug etoposide by almost doubling the IC(50) in either BON or HEK293 cell lines. Acridine Orange 85-100 chloride voltage-gated channel 3 Homo sapiens 0-5 17530925-6 2007 The major off-targeting effect is mediated through p53 activation, as detected through the transferase-mediated dUTP nick end labeling assay, acridine orange, and p21 transcriptional activation assays. Acridine Orange 142-157 tumor protein p53 Danio rerio 51-54 17118377-1 2007 A simple, rapid and simultaneous determination of four types of amphoteric surfactants, i.e., C8, C10, C12, C14, C16 and C18-homologues of alkyldimethylamine N-oxide (AO), alkylamidopropylamine N-oxide (APAO), alkylbetaine (Bt) and alkylamidopropylbetaine (APB), was performed by using capillary electrophoresis (CE) with indirect UV detection. Acridine Orange 167-169 Bardet-Biedl syndrome 9 Homo sapiens 121-124 16814760-0 2006 Acridine Orange based platinum(II) complexes inducing cytotoxicity and cell cycle perturbation in spite of GSTP1 up-regulation. Acridine Orange 0-15 glutathione S-transferase pi 1 Homo sapiens 107-112 17112039-1 2006 The change in the UV-absorption spectrum and fluorescence spectrum of acridine orange(AO) due to the addition of surfactant dodecyi benzene sulfonic acid sodium sait (SDBS) and bovine serum albumin(BSA) was studied. Acridine Orange 70-85 albumin Homo sapiens 184-202 16707209-8 2006 An inverse correlation was observed between ABP mRNA levels and uptake of acridine orange by estradiol treated caput sperm chromatin. Acridine Orange 74-89 sex hormone binding globulin Rattus norvegicus 44-47 16740722-2 2006 Exposure of PC-3 and LNCaP cells to sulforaphane resulted in several specific features characteristic of autophagy, including appearance of membranous vacuoles in the cytoplasm as revealed by transmission electron microscopy and formation of acidic vesicular organelles as revealed by fluorescence microscopy following staining with the lysosomotropic agent acridine orange. Acridine Orange 358-373 chromobox 8 Homo sapiens 12-16 16513374-6 2006 Histone H1-mediated cellular effects, such as anchorage dependent growth and apoptosis, were assessed by colony formation assay, fluorescence microscopy after acridine orange/propidium iodide staining and DNA fragmentation analysis. Acridine Orange 159-174 H1.0 linker histone Homo sapiens 0-10 16475697-11 2006 DISCUSSION: Based on results of the in vivo and in vitro studies, it is suggested that malignant musculoskeletal tumors have a large deltapH between the pHi and the pHe or between the pHi and the vaculolar pH and also that a large ApH increases AO accumulation in tumors. Acridine Orange 245-247 glucose-6-phosphate isomerase Homo sapiens 153-156 16441371-0 2006 Mutagenic effect of acridine orange on the expression of penicillin G acylase and beta-lactamase in Escherichia coli. Acridine Orange 20-35 beta-lactamase Escherichia coli 82-96 16441371-1 2006 AIMS: The present work aimed to improve the production of penicillin G acylase (PGA) and reduce the beta-lactamase activity through acridine orange (AO) induced mutation in Escherichia coli. Acridine Orange 132-147 beta-lactamase Escherichia coli 100-114 16441371-1 2006 AIMS: The present work aimed to improve the production of penicillin G acylase (PGA) and reduce the beta-lactamase activity through acridine orange (AO) induced mutation in Escherichia coli. Acridine Orange 149-151 beta-lactamase Escherichia coli 100-114 16475697-13 2006 CONCLUSION: AO accumulation in musculoskeletal tumors was dependent on the ApH between the pHi and the pHe, or between the pHi and the vacuolar pH. Acridine Orange 12-14 acylaminoacyl-peptide hydrolase Homo sapiens 75-78 16475697-13 2006 CONCLUSION: AO accumulation in musculoskeletal tumors was dependent on the ApH between the pHi and the pHe, or between the pHi and the vacuolar pH. Acridine Orange 12-14 glucose-6-phosphate isomerase Homo sapiens 91-94 16475697-13 2006 CONCLUSION: AO accumulation in musculoskeletal tumors was dependent on the ApH between the pHi and the pHe, or between the pHi and the vacuolar pH. Acridine Orange 12-14 glucose-6-phosphate isomerase Homo sapiens 123-126 16077903-8 2005 (2) Changes in nucleus morphology was observed by AO staining nucleic and flow cytometry analysis, which showed that stable Bcl-XL siRNA transfectants have an increased spontaneous apoptosis (21.17%+/-1.26% vs. 1.19%+/-0.18% and 1.56%+/-0.15% respectively, P < 0.05 vs. negative siRNA or untreated control). Acridine Orange 50-52 BCL2 like 1 Homo sapiens 124-130 16313111-1 2005 OBJECTIVE: To explore the molecular biological mechanism of Arnebia Root oil (AO) in promoting the recovery of surface of wound by observing basic fibroblast growth factor (bFGF) mRNA expression in the wound tissue and healing rate of the wound. Acridine Orange 78-80 fibroblast growth factor 2 Homo sapiens 141-171 16313111-1 2005 OBJECTIVE: To explore the molecular biological mechanism of Arnebia Root oil (AO) in promoting the recovery of surface of wound by observing basic fibroblast growth factor (bFGF) mRNA expression in the wound tissue and healing rate of the wound. Acridine Orange 78-80 fibroblast growth factor 2 Homo sapiens 173-177 16139877-0 2005 SYBR Green I-induced fluorescence in cultured immune cells: a comparison with Acridine Orange. Acridine Orange 78-93 semenogelin 1 Homo sapiens 0-12 16177561-4 2005 We also found that several randomly chosen acridine derivatives, including 9-aminoacridine, amsacrine, quinacrine and acridine orange, induced p53 transcriptional activity. Acridine Orange 118-133 tumor protein p53 Homo sapiens 143-146 16083584-6 2005 Acridine Orange staining and FCM analysis showed that Ad-Asc-myc could induce apoptosis of transfected cells, which was enhanced by the treatment of cisplatin cell. Acridine Orange 0-15 PYD and CARD domain containing Homo sapiens 57-60 16083584-6 2005 Acridine Orange staining and FCM analysis showed that Ad-Asc-myc could induce apoptosis of transfected cells, which was enhanced by the treatment of cisplatin cell. Acridine Orange 0-15 MYC proto-oncogene, bHLH transcription factor Homo sapiens 61-64 16241060-0 2005 [Determination of vitamin B12 concentration by fluorescence quenching with acridine orange-rhodamine 6G energy transfer system]. Acridine Orange 75-90 NADH:ubiquinone oxidoreductase subunit B3 Homo sapiens 26-29 15802856-2 2005 This paper describes a single-laser flow cytometry, based on an immunomagnetic isolation technique in combination with acridine orange staining, to detect frequencies of micronucleated transferrin-receptor positive reticulocytes from human peripheral blood. Acridine Orange 119-134 transferrin Homo sapiens 185-196 15494127-10 2004 Mass spectrometric analyses also showed that minor groove-binding ligands berenil, netropsin, and distamycin and the intercalating ligand acridine orange destabilize the (TTC)6. Acridine Orange 138-153 tetratricopeptide repeat domain 6 Homo sapiens 171-176 15753158-5 2004 The presence of hTTP substantially increases the rate of AO-alpha-tocopherol transfer over the uncatalyzed spontaneous rate. Acridine Orange 57-59 ZFP36 ring finger protein Homo sapiens 16-20 15107803-6 2004 In contrast, a marked decrement was observed in adjacent hippocampal sections stained for silver stain and acridine orange, both at the level of the regional dissection and at the CA1 neuron population level. Acridine Orange 107-122 carbonic anhydrase 1 Homo sapiens 180-183 15217796-5 2004 In controls, tissue Po2 in the risk region (RR) rose early in Rep and then fell to Occl levels, whereas in AO-treated animals, myocardial Po2 remained above baseline. Acridine Orange 107-109 PO2 Sus scrofa 138-141 15336597-2 2004 At 72 h of culture, 0.01, 0.1, and 1.0 nM IGF-II produced a dose-dependent increase in apoptosis assayed by TdT-mediated dUTP-biotin nick end labeling (TUNEL) and confirmed with acridine orange-ethidium bromide staining. Acridine Orange 178-193 insulin-like growth factor 2 Mus musculus 42-48 15336597-2 2004 At 72 h of culture, 0.01, 0.1, and 1.0 nM IGF-II produced a dose-dependent increase in apoptosis assayed by TdT-mediated dUTP-biotin nick end labeling (TUNEL) and confirmed with acridine orange-ethidium bromide staining. Acridine Orange 178-193 deoxynucleotidyltransferase, terminal Mus musculus 108-111 12812521-4 2003 Of the compounds investigated, incubation of human amylin with a 20-fold molar excess of either Congo Red or Acridine Orange resulted in significant inhibition in the rate of amyloid formation. Acridine Orange 109-124 islet amyloid polypeptide Homo sapiens 51-57 15346654-7 2004 Cell survival assay and fluorescence double dyeing with acridine orange and ethidium bromide showed that DPI and APO, as well as superoxide dismutase (SOD) and catalase (CAT) concentration-dependently decreased the viability of undifferentiated HL-60 cells, whereas exogenous H2O2 can rescue the cells from death obviously. Acridine Orange 56-71 catalase Homo sapiens 170-173 15052685-3 2004 Then the apoptotic rate of HSCs treated with different doses of somatostatin for 72 h, was assayed by acridine orange/ethidium bromide fluorescent staining, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, transmission electron microscopy and flow cytometry, while the proliferation of HSCs was measured by MTT assay. Acridine Orange 102-117 somatostatin Rattus norvegicus 64-76 12834894-7 2003 Confocal laser scanning microscopy analysis of immunostained astroglial cells showed a mainly cytoplasmic localisation of the enzyme both in control and treated cultures; nevertheless, counterstaining with the nuclear dye acridine orange demonstrated the presence of tissue transglutaminase also into the nucleus of glutamate-exposed and 21 DIV cells. Acridine Orange 222-237 transglutaminase 2 Homo sapiens 267-290 11880335-5 2002 NHE3 transport activity was assayed as the rate of appearance of acridine orange (AO) from AO-loaded vesicles in response to an inwardly directed Na(+) gradient. Acridine Orange 65-80 solute carrier family 9 member A3 Rattus norvegicus 0-4 12803085-1 2003 The singlet excited-state quenching of Acridine Orange (AO) by methyl viologen (MV2+) and the non-steroidal anti-inflammatory drug Piroxicam (Prx), incorporated in sodium bis(2-ethylhexyl) sulfosuccinate (AOT)/isooctane/water and Triton X-100 (Trx-100)/cyclohexane-hexanol/water (w/o) microemulsions, was followed by steady- and transient-state fluorescence. Acridine Orange 56-58 thioredoxin Homo sapiens 244-247 14510425-1 2003 Novel bis-acridine orange (1) was synthesized from Fmoc-Lys(Boc)-OH and Fmoc-Lys(AO)-OH (AO: acridine orange), with the 9-position of acridine orange (AO) linked to the epsilon-amino moiety of lysine, on the peptide synthesizer. Acridine Orange 10-25 BOC cell adhesion associated, oncogene regulated Homo sapiens 60-63 12201224-8 2002 Serum IGF-I levels were less than -1.96 SD in 43 of 56 (77%) patients with adult GHD (35/43 (81%) for CO and 8/13 (62%) for AO) and more than +1.96 SD in 42 of 43 (98%) patients with acromegaly, respectively. Acridine Orange 124-126 insulin like growth factor 1 Homo sapiens 6-11 12201224-9 2002 Serum IGFBP-3 levels were less than -1.96 SD in 51 of 56 (91%) patients with adult GHD (42/43 (98%) for CO and 9/13 (69%) for AO) and more than +1.96 SD in 31 of 43 (72%) patients with acromegaly, respectively. Acridine Orange 126-128 insulin like growth factor binding protein 3 Homo sapiens 6-13 11880335-5 2002 NHE3 transport activity was assayed as the rate of appearance of acridine orange (AO) from AO-loaded vesicles in response to an inwardly directed Na(+) gradient. Acridine Orange 82-84 solute carrier family 9 member A3 Rattus norvegicus 0-4 11923566-9 2002 Mean percent infarct size (area of necrosis)/(area at risk), quantitative post-mortem hemorrhage score, and myocardial myeloperoxidase levels at 3 hours of reperfusion were significantly less in the AO group (ANOVA, p < 0.05), but not in the HFO group, compared to normoxemic groups. Acridine Orange 199-201 myeloperoxidase Sus scrofa 119-134 11851353-8 2002 When the endothelial cells were preloaded with acridine orange, washed and resuspended in buffer containing 9-AAP, the dark orange-labeled vesicles observed with acridine orange alone became increasingly lighter with time. Acridine Orange 47-62 serpin family F member 2 Homo sapiens 110-113 11851353-8 2002 When the endothelial cells were preloaded with acridine orange, washed and resuspended in buffer containing 9-AAP, the dark orange-labeled vesicles observed with acridine orange alone became increasingly lighter with time. Acridine Orange 162-177 serpin family F member 2 Homo sapiens 110-113