PMID-sentid Pub_year Sent_text compound_name comp_offset prot_official_name organism prot_offset 9099730-3 1997 We report herein that DL-threo-dihydrosphingosine (DHS), a competitive inhibitor of sphingosine kinase that prevents PDGF-induced SPP formation, specifically inhibited the activation of two cyclin-dependent kinases (p34(cdc2) kinase and Cdk2 kinase) induced by PDGF, but not by EGF. dhs 51-54 cyclin dependent kinase 2 Homo sapiens 237-241 10079079-3 1999 In this study, we found a binding factor which recognized a unique palindrome sequence (DHS-22) in the region of the DHS II site of the HLA-DR alpha gene in THP-1 cells and HeLa cells. dhs 88-91 major histocompatibility complex, class II, DR alpha Homo sapiens 136-148 10079079-6 1999 Thus, we isolated the DHS-22 binding factor from bovine brain nuclear extracts and finally identified it as NF90 on the basis of molecular mass analysis of Lys-C-digested fragments and amino acid sequences of the two peptides of the trypsin-digested binding protein. dhs 22-25 interleukin enhancer binding factor 3 Homo sapiens 108-112 10079079-7 1999 The DHS-22 binding protein(s) in THP-1 cells is (are) further confirmed by reactivity to an antibody against NF90, and we have demonstrated that the GST fusion protein of NF90 interacts with DHS-22 by electrophoretic gel mobility shift assay (EMSA). dhs 4-7 interleukin enhancer binding factor 3 Homo sapiens 109-113 10079079-7 1999 The DHS-22 binding protein(s) in THP-1 cells is (are) further confirmed by reactivity to an antibody against NF90, and we have demonstrated that the GST fusion protein of NF90 interacts with DHS-22 by electrophoretic gel mobility shift assay (EMSA). dhs 4-7 interleukin enhancer binding factor 3 Homo sapiens 171-175 9099730-3 1997 We report herein that DL-threo-dihydrosphingosine (DHS), a competitive inhibitor of sphingosine kinase that prevents PDGF-induced SPP formation, specifically inhibited the activation of two cyclin-dependent kinases (p34(cdc2) kinase and Cdk2 kinase) induced by PDGF, but not by EGF. dhs 51-54 epidermal growth factor Homo sapiens 278-281 9099730-5 1997 DHS also markedly reduced PDGF-stimulated but not EGF-stimulated mitogen-activated protein kinase activity and DNA binding activity of activator protein-1. dhs 0-3 JunD proto-oncogene, AP-1 transcription factor subunit Homo sapiens 135-154 8887683-5 1996 Our analysis shows that the ecdysone response element is necessary but not sufficient for full developmental expression of hsp23 in the late third instar and that there is, indeed, another regulatory element, in the vicinity of DHS-1400. dhs 228-231 Heat shock protein 23 Drosophila melanogaster 123-128 9110297-8 1997 The DHs reacted with AE-1 and anti-CK-19 starting at Day 1, but reactivity to HepPar 1 started only at Day 4 post injury. dhs 4-7 solute carrier family 4 member 1 (Diego blood group) Homo sapiens 21-25 9110297-8 1997 The DHs reacted with AE-1 and anti-CK-19 starting at Day 1, but reactivity to HepPar 1 started only at Day 4 post injury. dhs 4-7 keratin 19 Homo sapiens 35-40 2054379-4 1991 Furthermore, in the 10 mM EB-treated nuclei, the pattern of DNAse I hypersensitive sites (DHS) around the chicken beta A-globin gene were significantly altered, i.e., the original set was replaced by a new set of DHS. dhs 90-93 hemoglobin subunit epsilon 1 Gallus gallus 114-127 8871649-2 1996 DNase I hypersensitive site (DHS) mapping revealed that the perforin locus contained six DHS within 7.0 kb of the 5" upstream sequence (-7.0 kb) and two DHS in intron 2. dhs 29-32 deoxyribonuclease I Mus musculus 0-7 34464302-7 2021 DHS and PFN both increased hs-IL-6 (143.81 +- 89.12 and 94.13 +- 67.14, respectively), CRP (98.84 +- 31.81 and 104.4 +- 31.80, respectively), and CK (400.8 +- 31.81 and 250.7 +- 31.80, respectively) 24 hours postoperatively. dhs 0-3 C-reactive protein Homo sapiens 87-90 34302525-8 2021 CDI comparison showed significantly higher scores of DSN in C3/4, C4/5, C5/6, and C6/7; sclerosis in C5/6 and C6/7; and osteophyte formation in C4/5, C5/6, and C6/7 in DHS group than in CSM group. dhs 168-171 interferon induced protein with tetratricopeptide repeats 1 Homo sapiens 60-76 34477311-3 2022 Lymphedema was diagnosed in a 36-year-old man from a three-generation DHS family, with a PIEZO1-allele harboring 3 missense mutations in cis. dhs 70-73 piezo type mechanosensitive ion channel component 1 Homo sapiens 89-95 34464302-7 2021 DHS and PFN both increased hs-IL-6 (143.81 +- 89.12 and 94.13 +- 67.14, respectively), CRP (98.84 +- 31.81 and 104.4 +- 31.80, respectively), and CK (400.8 +- 31.81 and 250.7 +- 31.80, respectively) 24 hours postoperatively. dhs 0-3 cytidine/uridine monophosphate kinase 1 Homo sapiens 146-148 34056129-11 2020 LDH, IL-1beta, and IL-6 levels were higher in the DHS group compared with the other two groups at 3 months after surgery (P<0.05). dhs 50-53 interleukin 1 alpha Homo sapiens 5-13 34056129-11 2020 LDH, IL-1beta, and IL-6 levels were higher in the DHS group compared with the other two groups at 3 months after surgery (P<0.05). dhs 50-53 interleukin 6 Homo sapiens 19-23 34056129-12 2020 From 3 to 6 months after surgery, the TNF-alpha level was high in the DHS and RIMN groups (P<0.05). dhs 70-73 tumor necrosis factor Homo sapiens 38-47 32700588-7 2020 Subjects with HLA-B*13:01 were susceptible to developing DHS compared to dapsone-tolerant controls (odds ratio [OR]: 73.667) and the Korean general population (OR: 139.500). dhs 57-60 major histocompatibility complex, class I, B Homo sapiens 14-19 33064728-6 2020 The aim of this study is to validate HLA-B*13:01, a previously discovered biomarker for DHS in the Chinese population, as a biomarker for DHS in the Papua population.This is a case-control study of 34 leprosy patients who presented themselves with DHS (case subjects) and 52 leprosy patients without DHS (control subjects). dhs 88-91 major histocompatibility complex, class I, B Homo sapiens 37-42 33064728-11 2020 The results of HLA-typing showed that HLA-B*13:01 was the most significant allele associated with DHS, with odds ratio = 233.64 and P-value = 7.11x10-9, confirming the strong association of HLA-B*13:01 to DHS in the Papua population. dhs 98-101 major histocompatibility complex, class I, B Homo sapiens 38-43 33064728-11 2020 The results of HLA-typing showed that HLA-B*13:01 was the most significant allele associated with DHS, with odds ratio = 233.64 and P-value = 7.11x10-9, confirming the strong association of HLA-B*13:01 to DHS in the Papua population. dhs 98-101 major histocompatibility complex, class I, B Homo sapiens 190-195 33064728-11 2020 The results of HLA-typing showed that HLA-B*13:01 was the most significant allele associated with DHS, with odds ratio = 233.64 and P-value = 7.11x10-9, confirming the strong association of HLA-B*13:01 to DHS in the Papua population. dhs 205-208 major histocompatibility complex, class I, B Homo sapiens 38-43 33064728-11 2020 The results of HLA-typing showed that HLA-B*13:01 was the most significant allele associated with DHS, with odds ratio = 233.64 and P-value = 7.11x10-9, confirming the strong association of HLA-B*13:01 to DHS in the Papua population. dhs 205-208 major histocompatibility complex, class I, B Homo sapiens 190-195 33064728-13 2020 HLA-B*13:01 is validated as a biomarker for DHS in leprosy patients in Papua, Indonesia, and can potentially be a good predictor of DHS to help prevent this condition in the future. dhs 44-47 major histocompatibility complex, class I, B Homo sapiens 0-5 33064728-13 2020 HLA-B*13:01 is validated as a biomarker for DHS in leprosy patients in Papua, Indonesia, and can potentially be a good predictor of DHS to help prevent this condition in the future. dhs 132-135 major histocompatibility complex, class I, B Homo sapiens 0-5 32700588-10 2020 CONCLUSIONS: Similar to previous Asian population studies, HLA-B*13:01 is significantly associated with the risk of DHS in Korea. dhs 116-119 major histocompatibility complex, class I, B Homo sapiens 59-64 30040918-5 2018 Importantly, DHS also reduces the number of OXT+ cells in the PVN of adult male, but not female, rats. dhs 13-16 oxytocin/neurophysin I prepropeptide Rattus norvegicus 44-47 31162551-9 2019 RESULTS: Galectin-3 levels were associated with diabetes prevalence in DHS-1 [OR 1.56 per SD change in log-galectin (95% CI 1.41 to 1.73)] and DHS-2 [OR 1.86 (95% CI 1.67 to 2.06)]. dhs 71-74 galectin 3 Homo sapiens 9-19 31162551-10 2019 Galectin-3 levels in DHS-1 also associated with incident diabetes mellitus over the 7.1 (interquartile range 6.6 to 7.6)-year follow-up period [OR 1.34 (95% CI 1.14 to 1.58)]. dhs 21-24 galectin 3 Homo sapiens 0-10 30518875-0 2019 DHS (trans-4,4"-dihydroxystilbene) suppresses DNA replication and tumor growth by inhibiting RRM2 (ribonucleotide reductase regulatory subunit M2). dhs 0-3 ribonucleotide reductase regulatory subunit M2 Homo sapiens 93-97 30518875-0 2019 DHS (trans-4,4"-dihydroxystilbene) suppresses DNA replication and tumor growth by inhibiting RRM2 (ribonucleotide reductase regulatory subunit M2). dhs 0-3 ribonucleotide reductase regulatory subunit M2 Homo sapiens 99-145 30518875-8 2019 Molecular docking analysis identified the RRM2 (ribonucleotide reductase regulatory subunit M2) of RNR as a direct target of DHS. dhs 125-128 ribonucleotide reductase regulatory subunit M2 Homo sapiens 42-46 30518875-8 2019 Molecular docking analysis identified the RRM2 (ribonucleotide reductase regulatory subunit M2) of RNR as a direct target of DHS. dhs 125-128 ribonucleotide reductase regulatory subunit M2 Homo sapiens 48-94 30518875-9 2019 At the molecular level, DHS induced cyclin F-mediated down-regulation of RRM2 by the proteasome. dhs 24-27 cyclin F Homo sapiens 36-44 30518875-9 2019 At the molecular level, DHS induced cyclin F-mediated down-regulation of RRM2 by the proteasome. dhs 24-27 ribonucleotide reductase regulatory subunit M2 Homo sapiens 73-77 30518875-13 2019 Thus, DHS is a novel anti-cancer agent that targets RRM2 with therapeutic potential either alone or in combination with other agents to arrest cancer development. dhs 6-9 ribonucleotide reductase regulatory subunit M2 Homo sapiens 52-56 30257322-8 2018 Mechanistically, DHS modulated the expressions of several key metastasis regulating proteins e.g., MMP-2/9, N-cadherin, E-cadherin and survivin. dhs 17-20 matrix metallopeptidase 2 Mus musculus 99-106 30477471-9 2018 Alpha actinin skeletal muscle 2 (ACTN2) was upregulated 3.3-fold for ADSC/Mb and 1.7-fold for BMSC/Mb after myogenic induction by group 3 serum-free medium when compared to stimulation with DHS. dhs 190-193 actinin alpha 2 Rattus norvegicus 33-38 30477471-12 2018 Myosin heavy chain 2, ACTN2, and MYOG as late myogenic markers, showed higher gene expression after long term stimulation with DHS compared to serum-free stimulation, especially in BMSC/Mb co-cultures. dhs 127-130 myosin heavy chain 2 Rattus norvegicus 0-20 30477471-12 2018 Myosin heavy chain 2, ACTN2, and MYOG as late myogenic markers, showed higher gene expression after long term stimulation with DHS compared to serum-free stimulation, especially in BMSC/Mb co-cultures. dhs 127-130 actinin alpha 2 Rattus norvegicus 22-27 30477471-12 2018 Myosin heavy chain 2, ACTN2, and MYOG as late myogenic markers, showed higher gene expression after long term stimulation with DHS compared to serum-free stimulation, especially in BMSC/Mb co-cultures. dhs 127-130 myogenin Rattus norvegicus 33-37 30257322-8 2018 Mechanistically, DHS modulated the expressions of several key metastasis regulating proteins e.g., MMP-2/9, N-cadherin, E-cadherin and survivin. dhs 17-20 cadherin 2 Mus musculus 108-118 30257322-8 2018 Mechanistically, DHS modulated the expressions of several key metastasis regulating proteins e.g., MMP-2/9, N-cadherin, E-cadherin and survivin. dhs 17-20 cadherin 1 Mus musculus 120-130 30257322-8 2018 Mechanistically, DHS modulated the expressions of several key metastasis regulating proteins e.g., MMP-2/9, N-cadherin, E-cadherin and survivin. dhs 17-20 baculoviral IAP repeat-containing 5 Mus musculus 135-143 28464455-2 2017 These studies allowed us to assign the functions for all four DHs, identifying DH10 as the dedicated dehydratase that catalyzes the dehydration of the C17 hydroxy group during iso-MGS biosynthesis. dhs 62-65 cytokine like 1 Homo sapiens 151-154 29249319-11 2018 Finally, the addition of ePL to LPS-stimulated monocytes in the presence of various concentrations of DHS resulted to statistically significant decrease of TNF-alpha and IL-1beta compared to the control groups. dhs 102-105 tumor necrosis factor Equus caballus 156-165 29249319-11 2018 Finally, the addition of ePL to LPS-stimulated monocytes in the presence of various concentrations of DHS resulted to statistically significant decrease of TNF-alpha and IL-1beta compared to the control groups. dhs 102-105 interleukin-1 beta Equus caballus 170-178 28575580-4 2017 Throughout the evaluation period of 550 days, the DHS reactor at a hydraulic retention time of 3 h showed better performance than the existing oxidation ditch process in the removal of organic carbon (COD removal rate = 80-83% and BOD removal rate = 91%), nitrogen compounds (total nitrogen removal rate = 45-51% and NH4+-N removal rate = 95-98%), and low excess sludge production (0.04 gTS/gCOD removed). dhs 50-53 GTS Homo sapiens 387-390 29896265-7 2018 The contents of TGF-beta2 at 1, 7 and 15 days in the PFNA group were higher than those at the identical time-points in the DHS group (P<0.01). dhs 123-126 transforming growth factor beta 2 Homo sapiens 16-25 29896265-10 2018 PFNA fixation is superior to DHS fixation, which may be associated with the higher level of TGF-beta2 expression in comparison with that in the DHS group. dhs 29-32 transforming growth factor beta 2 Homo sapiens 92-101 29176792-5 2017 Nearly half of all DHS sites (both constitutive and dynamic) overlap binding events of the bone-essential RUNX2 and/or the chromatin-related CTCF transcription factors. dhs 19-22 runt related transcription factor 2 Mus musculus 106-111 29176792-5 2017 Nearly half of all DHS sites (both constitutive and dynamic) overlap binding events of the bone-essential RUNX2 and/or the chromatin-related CTCF transcription factors. dhs 19-22 CCCTC-binding factor Mus musculus 141-145 27542713-3 2016 DHS treatment, despite inducing expression of glutathione peroxidases (GPx1, GPx2, and GPx4) in spleen and intestine, did not protect against radiation-induced acute (10-day) haematopoietic and gastrointestinal toxicities. dhs 0-3 glutathione peroxidase 4 Mus musculus 87-91 28035317-2 2016 We found that two hematopoietic specific DNase I hypersensitive (DHS) sites (proximal DHS-A, and distal DHS-B) which had high interaction frequencies with the proximal WASp promoter indicating potential regulatory activity for these DHS sites. dhs 65-68 WASP actin nucleation promoting factor Homo sapiens 168-172 28035317-2 2016 We found that two hematopoietic specific DNase I hypersensitive (DHS) sites (proximal DHS-A, and distal DHS-B) which had high interaction frequencies with the proximal WASp promoter indicating potential regulatory activity for these DHS sites. dhs 86-89 WASP actin nucleation promoting factor Homo sapiens 168-172 28035317-2 2016 We found that two hematopoietic specific DNase I hypersensitive (DHS) sites (proximal DHS-A, and distal DHS-B) which had high interaction frequencies with the proximal WASp promoter indicating potential regulatory activity for these DHS sites. dhs 86-89 WASP actin nucleation promoting factor Homo sapiens 168-172 28035317-2 2016 We found that two hematopoietic specific DNase I hypersensitive (DHS) sites (proximal DHS-A, and distal DHS-B) which had high interaction frequencies with the proximal WASp promoter indicating potential regulatory activity for these DHS sites. dhs 86-89 WASP actin nucleation promoting factor Homo sapiens 168-172 28035317-7 2016 Subsequently, the hematopoietic specific DHS sites enhanced luciferase expression from the proximal WASp promoter in all hematopoietic cells we tested. dhs 41-44 WASP actin nucleation promoting factor Homo sapiens 100-104 29088756-5 2017 DHS (20 muM) induced mitochondrial membrane permeabilization (MMP) in the cells, as revealed from JC-1 staining, cytochrome c and ApaF1 release and caspases-9/3 activation. dhs 0-3 latexin Homo sapiens 8-11 29088756-5 2017 DHS (20 muM) induced mitochondrial membrane permeabilization (MMP) in the cells, as revealed from JC-1 staining, cytochrome c and ApaF1 release and caspases-9/3 activation. dhs 0-3 cytochrome c, somatic Homo sapiens 113-125 29088756-5 2017 DHS (20 muM) induced mitochondrial membrane permeabilization (MMP) in the cells, as revealed from JC-1 staining, cytochrome c and ApaF1 release and caspases-9/3 activation. dhs 0-3 apoptotic peptidase activating factor 1 Homo sapiens 130-135 29088756-6 2017 DHS also induced lysosomal membrane permeabilization (LMP) to release cathepsins B, L and D, and the cathepsins inhibitors partially reduced MMP/caspase-3 activation. dhs 0-3 caspase 3 Homo sapiens 145-154 29088756-7 2017 The ROS, produced by DHS activated the p38 and JNK MAPKs to augment the BAX activity and BID-cleavage, and induce LMP and MMP in the cells. dhs 21-24 mitogen-activated protein kinase 14 Homo sapiens 39-42 29088756-7 2017 The ROS, produced by DHS activated the p38 and JNK MAPKs to augment the BAX activity and BID-cleavage, and induce LMP and MMP in the cells. dhs 21-24 mitogen-activated protein kinase 8 Homo sapiens 47-50 29088756-7 2017 The ROS, produced by DHS activated the p38 and JNK MAPKs to augment the BAX activity and BID-cleavage, and induce LMP and MMP in the cells. dhs 21-24 BCL2 associated X, apoptosis regulator Homo sapiens 72-75 30179393-4 2016 DHS reduced mitochondrial membrane permeabilization and deactivated reactive oxygen species (ROS)-mediated caspase-3 activation in the H2O2-treated PC12 cells. dhs 0-3 caspase 3 Rattus norvegicus 107-116 27542713-5 2016 The anti-inflammatory effect of DHS was associated with reductions in lipid peroxidation, expression of pro-inflammatory genes such as Icam-1, Ccl-2, and iNos-2, and subsequent infiltration of inflammatory cells. dhs 32-35 intercellular adhesion molecule 1 Mus musculus 135-141 27542713-5 2016 The anti-inflammatory effect of DHS was associated with reductions in lipid peroxidation, expression of pro-inflammatory genes such as Icam-1, Ccl-2, and iNos-2, and subsequent infiltration of inflammatory cells. dhs 32-35 chemokine (C-C motif) ligand 2 Mus musculus 143-148 26435056-7 2015 Both the endogenous and transgenic Oct4 enhancer interacting loci were enriched in the open nucleus compartment, which is associated with active histone marks (H3K4me1, H3K27ac, H3K4me3 and H3K9ac), active cis-regulatory sequences (DNA hypersensitivity sites (DHS)), 5-hydroxymethylcytosine (5-hmc), and early DNA replication domains. dhs 260-263 POU domain, class 5, transcription factor 1, related sequence 1 Mus musculus 35-39 27087856-8 2016 We found PU.1 increases binding at opened DHS sites with HDACi treatment by ChIP-seq, but PU.1 knockdown by shRNA fails to block the chromatin accessibility and expression changes. dhs 42-45 Spi-1 proto-oncogene Homo sapiens 9-13 25492239-4 2016 Herein, for the first time, we report the efficacy of silibinin and its oxidation product 2,3-dehydrosilibinin (DHS) against BCC both in vitro and in vivo using ASZ (p53 mutated) and BSZ (p53 deleted) cell lines derived from murine BCC tumors. dhs 112-115 transformation related protein 53, pseudogene Mus musculus 166-169 25492239-4 2016 Herein, for the first time, we report the efficacy of silibinin and its oxidation product 2,3-dehydrosilibinin (DHS) against BCC both in vitro and in vivo using ASZ (p53 mutated) and BSZ (p53 deleted) cell lines derived from murine BCC tumors. dhs 112-115 transformation related protein 53, pseudogene Mus musculus 188-191 25492239-7 2016 More importantly, in an ectopic allograft model, oral administration of silibinin and DHS (200 mg/kg body weight) strongly inhibited the ASZ tumor growth by 44% and 71% (P < 0.05), respectively, and decreased the expression of proliferation biomarkers (PCNA and cyclin D1) as well as NF-kappaB p50 and c-Fos in the tumor tissues. dhs 86-89 proliferating cell nuclear antigen Mus musculus 256-260 25492239-7 2016 More importantly, in an ectopic allograft model, oral administration of silibinin and DHS (200 mg/kg body weight) strongly inhibited the ASZ tumor growth by 44% and 71% (P < 0.05), respectively, and decreased the expression of proliferation biomarkers (PCNA and cyclin D1) as well as NF-kappaB p50 and c-Fos in the tumor tissues. dhs 86-89 cyclin D1 Mus musculus 265-274 25492239-7 2016 More importantly, in an ectopic allograft model, oral administration of silibinin and DHS (200 mg/kg body weight) strongly inhibited the ASZ tumor growth by 44% and 71% (P < 0.05), respectively, and decreased the expression of proliferation biomarkers (PCNA and cyclin D1) as well as NF-kappaB p50 and c-Fos in the tumor tissues. dhs 86-89 nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 Mus musculus 297-300 25492239-7 2016 More importantly, in an ectopic allograft model, oral administration of silibinin and DHS (200 mg/kg body weight) strongly inhibited the ASZ tumor growth by 44% and 71% (P < 0.05), respectively, and decreased the expression of proliferation biomarkers (PCNA and cyclin D1) as well as NF-kappaB p50 and c-Fos in the tumor tissues. dhs 86-89 FBJ osteosarcoma oncogene Mus musculus 305-310 26605532-9 2015 Analysis of the DHS sequences uncovers one mutation (chr18: 52417839G>C) in the tumour cells of a patient with follicular thyroid carcinoma, which affects the binding of the tumour suppressor protein p53 and correlates with decreased expression of its target gene TXNL1. dhs 16-19 tumor protein p53 Homo sapiens 203-206 26605532-9 2015 Analysis of the DHS sequences uncovers one mutation (chr18: 52417839G>C) in the tumour cells of a patient with follicular thyroid carcinoma, which affects the binding of the tumour suppressor protein p53 and correlates with decreased expression of its target gene TXNL1. dhs 16-19 thioredoxin like 1 Homo sapiens 267-272 22688402-12 2012 In contrast, the postoperative blood level of IL-6 in patients operated with DHS conv technique (78.41 +- 67.04 pg/ml) was on average higher than in patients operated by DHS MIO technique (39.02 +- 37.36 pg/ml), the mean difference being 39.39 pg/ml [95 % confidence interval (CI) 12.65-66.13 pg/ml; p = 0.0045]. dhs 77-80 interleukin 6 Homo sapiens 46-50 24530665-6 2014 RESULTS: The 99th percentile values for the hs-cTnT assay in DHS, ARIC, and CHS were 18, 22, and 36 ng/l (subcohort 1) and 14, 21, and 28 ng/l (subcohort 2), respectively. dhs 61-64 troponin T2, cardiac type Homo sapiens 47-51 24465674-8 2014 With this design, we detected a DHS site at the CD4 gene in two CD4 T cell populations using as few as 2x10(4) cells. dhs 32-35 CD4 molecule Homo sapiens 48-51 24465674-8 2014 With this design, we detected a DHS site at the CD4 gene in two CD4 T cell populations using as few as 2x10(4) cells. dhs 32-35 CD4 molecule Homo sapiens 64-67 24465674-9 2014 We further validated this method by detecting a DHS site of the IL-4 gene that is specifically present in type 2 but not type 1 T helper cells. dhs 48-51 interleukin 4 Homo sapiens 64-68 24006278-10 2013 In silico prediction of transcription factor binding sites that were overrepresented in epididymis-selective DHS identified epithelial transcription factors, including ELF5 and ELF3, the androgen receptor, Pax2, and Sox9, as components of epididymis transcriptional networks. dhs 109-112 E74 like ETS transcription factor 5 Homo sapiens 168-172 24006278-10 2013 In silico prediction of transcription factor binding sites that were overrepresented in epididymis-selective DHS identified epithelial transcription factors, including ELF5 and ELF3, the androgen receptor, Pax2, and Sox9, as components of epididymis transcriptional networks. dhs 109-112 E74 like ETS transcription factor 3 Homo sapiens 177-181 24006278-10 2013 In silico prediction of transcription factor binding sites that were overrepresented in epididymis-selective DHS identified epithelial transcription factors, including ELF5 and ELF3, the androgen receptor, Pax2, and Sox9, as components of epididymis transcriptional networks. dhs 109-112 androgen receptor Homo sapiens 187-204 24006278-10 2013 In silico prediction of transcription factor binding sites that were overrepresented in epididymis-selective DHS identified epithelial transcription factors, including ELF5 and ELF3, the androgen receptor, Pax2, and Sox9, as components of epididymis transcriptional networks. dhs 109-112 paired box 2 Homo sapiens 206-210 24006278-10 2013 In silico prediction of transcription factor binding sites that were overrepresented in epididymis-selective DHS identified epithelial transcription factors, including ELF5 and ELF3, the androgen receptor, Pax2, and Sox9, as components of epididymis transcriptional networks. dhs 109-112 SRY-box transcription factor 9 Homo sapiens 216-220 23689137-1 2013 An airway-selective DNase-hypersensitive site (DHS) at kb -35 (DHS-35kb) 5" to the cystic fibrosis transmembrane conductance regulator (CFTR) gene is evident in many lung cell lines and primary human tracheal epithelial cells but is absent from intestinal epithelia. dhs 47-50 CF transmembrane conductance regulator Homo sapiens 83-134 23689137-1 2013 An airway-selective DNase-hypersensitive site (DHS) at kb -35 (DHS-35kb) 5" to the cystic fibrosis transmembrane conductance regulator (CFTR) gene is evident in many lung cell lines and primary human tracheal epithelial cells but is absent from intestinal epithelia. dhs 47-50 CF transmembrane conductance regulator Homo sapiens 136-140 23689137-1 2013 An airway-selective DNase-hypersensitive site (DHS) at kb -35 (DHS-35kb) 5" to the cystic fibrosis transmembrane conductance regulator (CFTR) gene is evident in many lung cell lines and primary human tracheal epithelial cells but is absent from intestinal epithelia. dhs 63-66 CF transmembrane conductance regulator Homo sapiens 83-134 23689137-1 2013 An airway-selective DNase-hypersensitive site (DHS) at kb -35 (DHS-35kb) 5" to the cystic fibrosis transmembrane conductance regulator (CFTR) gene is evident in many lung cell lines and primary human tracheal epithelial cells but is absent from intestinal epithelia. dhs 63-66 CF transmembrane conductance regulator Homo sapiens 136-140 23689137-3 2013 We now define a 350-bp region within DHS-35kb which has full enhancer activity and binds interferon regulatory factor 1 (IRF1) and nuclear factor Y (NF-Y) in vitro and in vivo. dhs 37-40 interferon regulatory factor 1 Homo sapiens 89-119 23689137-3 2013 We now define a 350-bp region within DHS-35kb which has full enhancer activity and binds interferon regulatory factor 1 (IRF1) and nuclear factor Y (NF-Y) in vitro and in vivo. dhs 37-40 interferon regulatory factor 1 Homo sapiens 121-125 23689137-5 2013 NF-Y is critical for maintenance of H3K4me1 enrichment at DHS-35kb since depletion of NF-YA, a subunit of NF-Y, reduces H3K4me1 enrichment at this site. dhs 58-61 nuclear transcription factor Y subunit alpha Homo sapiens 86-91 23689137-8 2013 Our results reveal that the DHS-35kb airway-selective enhancer element plays a pivotal role in regulation of CFTR expression by two independent regulatory mechanisms. dhs 28-31 CF transmembrane conductance regulator Homo sapiens 109-113 18062943-8 2008 Post mortem analyses revealed that DHS animals had a loss of oxytocin (OT)-containing cells in the paraventricular nucleus in the hypothalamus (PVN; p<0.05) as well as an increase in calcitonin-gene related peptide (CGRP; p<0.05, one tailed) processes in the central nucleus of the amygdala (CeA) on PND 198. dhs 35-38 calcitonin-related polypeptide alpha Rattus norvegicus 186-217 22063473-6 2011 RESULTS: The age-standardized incidence rate of RLS was 22/1000 person-years (p-y) (cumulative incidence over the follow-up: 9.1%) in DHS and 9/1000 p-y (cumulative incidence: 7.0%) in SHIP. dhs 134-137 RLS1 Homo sapiens 48-51 22063473-9 2011 The persistence of RLS symptoms from baseline to follow-up was 47.4% in DHS and 41.5% in SHIP. dhs 72-75 RLS1 Homo sapiens 19-22 19551581-11 2009 Cut-out was seen more in DHS (19 %, PCCP 2 %, PFN 4 %, p = 0.005). dhs 25-28 profilin family member 4 Homo sapiens 46-51 19782160-3 2009 We previously identified a number of intronic elements that were associated with DNase I hypersensitive sites (DHS) and bound the hepatocyte nuclear factor 1 (HNF1) transcription factor. dhs 111-114 HNF1 homeobox A Homo sapiens 130-157 19782160-3 2009 We previously identified a number of intronic elements that were associated with DNase I hypersensitive sites (DHS) and bound the hepatocyte nuclear factor 1 (HNF1) transcription factor. dhs 111-114 HNF1 homeobox A Homo sapiens 159-163 19782160-5 2009 Here we investigate DHS in introns 16 and 17a of the CFTR gene, which are evident in intestinal and pancreatic cell lines, and determine the transcription factors that interact with these sites. dhs 20-23 CF transmembrane conductance regulator Homo sapiens 53-57 19782160-7 2009 We demonstrate that though sequences within these DHS bind HNF1, CDX2, and PBX1 in vitro, only PBX1 show a robust in vivo interaction. dhs 50-53 HNF1 homeobox A Homo sapiens 59-63 19782160-7 2009 We demonstrate that though sequences within these DHS bind HNF1, CDX2, and PBX1 in vitro, only PBX1 show a robust in vivo interaction. dhs 50-53 caudal type homeobox 2 Homo sapiens 65-69 19782160-7 2009 We demonstrate that though sequences within these DHS bind HNF1, CDX2, and PBX1 in vitro, only PBX1 show a robust in vivo interaction. dhs 50-53 PBX homeobox 1 Homo sapiens 75-79 20329606-9 2009 CONCLUSIONS: Patients with sharp deteriorated CRF of DHS show higher plasma levels of CD62 P and ICAM-1 in comparing with the normal control. dhs 53-56 selectin P Homo sapiens 86-92 20329606-9 2009 CONCLUSIONS: Patients with sharp deteriorated CRF of DHS show higher plasma levels of CD62 P and ICAM-1 in comparing with the normal control. dhs 53-56 intercellular adhesion molecule 1 Homo sapiens 97-103 19129223-4 2009 Here we focus on a DHS at +6.8 kb from the CFTR translation end-point to evaluate its potential role in regulating expression of the gene. dhs 19-22 CF transmembrane conductance regulator Homo sapiens 43-47 19129223-5 2009 This DHS, which encompasses a consensus CTCF-binding site, was evident in primary human epididymis cells that express abundant CFTR mRNA. dhs 5-8 CCCTC-binding factor Homo sapiens 40-44 19129223-5 2009 This DHS, which encompasses a consensus CTCF-binding site, was evident in primary human epididymis cells that express abundant CFTR mRNA. dhs 5-8 CF transmembrane conductance regulator Homo sapiens 127-131 19129223-6 2009 We show by DNase I footprinting and electophoretic mobility shift assays that the cis-regulatory element within this DHS binds CTCF in vitro. dhs 117-120 CCCTC-binding factor Homo sapiens 127-131 19129223-8 2009 Moreover, we reveal that in primary epididymis cells, the +6.8 kb DHS interacts closely with the CFTR promoter, suggesting that the CFTR locus exists in a looped conformation, characteristic of an active chromatin hub. dhs 66-69 CF transmembrane conductance regulator Homo sapiens 97-101 19129223-8 2009 Moreover, we reveal that in primary epididymis cells, the +6.8 kb DHS interacts closely with the CFTR promoter, suggesting that the CFTR locus exists in a looped conformation, characteristic of an active chromatin hub. dhs 66-69 CF transmembrane conductance regulator Homo sapiens 132-136 18062943-8 2008 Post mortem analyses revealed that DHS animals had a loss of oxytocin (OT)-containing cells in the paraventricular nucleus in the hypothalamus (PVN; p<0.05) as well as an increase in calcitonin-gene related peptide (CGRP; p<0.05, one tailed) processes in the central nucleus of the amygdala (CeA) on PND 198. dhs 35-38 calcitonin-related polypeptide alpha Rattus norvegicus 219-223 18062943-8 2008 Post mortem analyses revealed that DHS animals had a loss of oxytocin (OT)-containing cells in the paraventricular nucleus in the hypothalamus (PVN; p<0.05) as well as an increase in calcitonin-gene related peptide (CGRP; p<0.05, one tailed) processes in the central nucleus of the amygdala (CeA) on PND 198. dhs 35-38 carcinoembryonic antigen gene family 4 Rattus norvegicus 298-301 17696881-6 2007 The DHS core at -20.9 kb binds CTCF (CCCTC-binding factor) both in vitro and in vivo; however, the +15.6 kb core appears to bind other factors. dhs 4-7 CCCTC-binding factor Homo sapiens 31-35 17696881-6 2007 The DHS core at -20.9 kb binds CTCF (CCCTC-binding factor) both in vitro and in vivo; however, the +15.6 kb core appears to bind other factors. dhs 4-7 CCCTC-binding factor Homo sapiens 37-57 17696881-7 2007 Histone-modification analysis across the CFTR locus highlights structural differences between the -20.9 kb and +15.6 kb DHS, further suggesting that these two insulator elements may operate by distinct mechanisms. dhs 120-123 CF transmembrane conductance regulator Homo sapiens 41-45 17382928-8 2007 CONCLUSION: Variation in the IRAK1 gene is associated with CRP concentration in Caucasian women in DHS. dhs 99-102 interleukin 1 receptor associated kinase 1 Homo sapiens 29-34 17382928-8 2007 CONCLUSION: Variation in the IRAK1 gene is associated with CRP concentration in Caucasian women in DHS. dhs 99-102 C-reactive protein Homo sapiens 59-62 15763622-9 2005 Also, with DHS the response in flux profile, by switching the current on and off, was shallower than that with HSC. dhs 11-14 fucosyltransferase 1 (H blood group) Homo sapiens 111-114 15195136-8 2004 Furthermore, we have found that ectopic expression of v-Myb in a myelomonocytic cell line is able to induce a DHS downstream of the C/EBPbeta gene, showing for the first time that v-Myb can affect chromatin structure. dhs 110-113 MYB proto-oncogene, transcription factor Gallus gallus 182-185 15881228-9 2005 Although TNF-alpha stimulated the AP-1-mediated expression of the monocyte chemoattractant JE/MCP-1, this stimulation was inhibited by DHS. dhs 135-138 tumor necrosis factor Mus musculus 9-18 15881228-9 2005 Although TNF-alpha stimulated the AP-1-mediated expression of the monocyte chemoattractant JE/MCP-1, this stimulation was inhibited by DHS. dhs 135-138 chemokine (C-C motif) ligand 2 Mus musculus 94-99 15567191-5 2004 These findings suggest that NO formation via iNOS activation may contribute to organ damage and that the selective iNOS inhibitor, AG, may be of interest as a therapeutic agent for neurological recovery following DHS. dhs 213-216 nitric oxide synthase 2 Rattus norvegicus 115-119 15195136-8 2004 Furthermore, we have found that ectopic expression of v-Myb in a myelomonocytic cell line is able to induce a DHS downstream of the C/EBPbeta gene, showing for the first time that v-Myb can affect chromatin structure. dhs 110-113 MYB proto-oncogene, transcription factor Gallus gallus 56-59 15195136-8 2004 Furthermore, we have found that ectopic expression of v-Myb in a myelomonocytic cell line is able to induce a DHS downstream of the C/EBPbeta gene, showing for the first time that v-Myb can affect chromatin structure. dhs 110-113 CCAAT/enhancer binding protein beta Gallus gallus 132-141 15195136-9 2004 Reporter gene experiments demonstrate that the downstream DHS acts as a Myb-dependent enhancing element in transiently as well as in stably transfected myelomonocytic cells. dhs 58-61 MYB proto-oncogene, transcription factor Gallus gallus 72-75 11856314-3 2002 We previously identified at least 12 clusters of DHS across the CFTR gene and here further evaluate DHS in introns 2, 3, 10, 16, 17a, 18, 20 and 21 to assess their functional importance in regulation of CFTR gene expression. dhs 49-52 CF transmembrane conductance regulator Homo sapiens 64-68 15181033-16 2004 When compared with cycles of younger control women, the cycles of the SWAN DHS participants had higher gonadotropins, lower total integrated Pdg, and E1c levels that were not different, which suggests that the ovary retains sensitivity to elevated FSH in the early menopausal transition. dhs 75-78 small nucleolar RNA, H/ACA box 73B Homo sapiens 150-153 12899723-7 2003 We conclude that screening of dHS patients for such a reduced allele expression in common ANK1 polymorphisms is an efficient procedure for the identification of candidates for frameshift/nonsense mutations in the ankyrin-1 gene. dhs 30-33 ankyrin 1 Homo sapiens 90-94 12899723-7 2003 We conclude that screening of dHS patients for such a reduced allele expression in common ANK1 polymorphisms is an efficient procedure for the identification of candidates for frameshift/nonsense mutations in the ankyrin-1 gene. dhs 30-33 ankyrin 1 Homo sapiens 213-222 12136238-4 2002 We previously described a DHS at +15.6 kb 3" to the CFTR gene, which showed tissue specificity in vivo and was evaluated as a candidate regulatory element for CFTR. dhs 26-29 CF transmembrane conductance regulator Homo sapiens 52-56 12136238-4 2002 We previously described a DHS at +15.6 kb 3" to the CFTR gene, which showed tissue specificity in vivo and was evaluated as a candidate regulatory element for CFTR. dhs 26-29 CF transmembrane conductance regulator Homo sapiens 159-163 11911476-3 2002 DHS VIII was highly AT-rich (75%) and was comprised of multiple sets of high mobility group (HMG)-I/Y binding site, two potential Special AT-rich Binding protein (SATB-1)-binding sites, and a long stretch of CTAT repeats, indicating that DHS VIII may relate to nuclear matrix-associated region (MAR). dhs 0-3 high mobility group AT-hook 1 Mus musculus 72-99 11856314-3 2002 We previously identified at least 12 clusters of DHS across the CFTR gene and here further evaluate DHS in introns 2, 3, 10, 16, 17a, 18, 20 and 21 to assess their functional importance in regulation of CFTR gene expression. dhs 100-103 CF transmembrane conductance regulator Homo sapiens 203-207 11856314-6 2002 Cell specificity of the DHS suggested a role for the DHS in introns 2 and 18 in CFTR expression in some pancreatic duct cells. dhs 24-27 CF transmembrane conductance regulator Homo sapiens 80-84 11856314-6 2002 Cell specificity of the DHS suggested a role for the DHS in introns 2 and 18 in CFTR expression in some pancreatic duct cells. dhs 53-56 CF transmembrane conductance regulator Homo sapiens 80-84 11856314-7 2002 Finally, regulatory elements at the DHS in introns 10 and 18 may contribute to upregulation of CFTR gene transcription by forskolin and mitomycin C, respectively. dhs 36-39 CF transmembrane conductance regulator Homo sapiens 95-99 10417323-6 1999 Further analysis of the 4574+15.6 kb DHS implicates the involvement of CCAAT-enhancer-binding protein (C/EBP), cAMP-response-element-binding protein (CREB)/activating transcription factor (ATF) and activator protein 1 (AP-1) family transcription factors at this regulatory element. dhs 37-40 CCAAT enhancer binding protein alpha Homo sapiens 71-101 10417323-6 1999 Further analysis of the 4574+15.6 kb DHS implicates the involvement of CCAAT-enhancer-binding protein (C/EBP), cAMP-response-element-binding protein (CREB)/activating transcription factor (ATF) and activator protein 1 (AP-1) family transcription factors at this regulatory element. dhs 37-40 glial cell derived neurotrophic factor Homo sapiens 189-192 10417323-6 1999 Further analysis of the 4574+15.6 kb DHS implicates the involvement of CCAAT-enhancer-binding protein (C/EBP), cAMP-response-element-binding protein (CREB)/activating transcription factor (ATF) and activator protein 1 (AP-1) family transcription factors at this regulatory element. dhs 37-40 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 198-217 10417323-6 1999 Further analysis of the 4574+15.6 kb DHS implicates the involvement of CCAAT-enhancer-binding protein (C/EBP), cAMP-response-element-binding protein (CREB)/activating transcription factor (ATF) and activator protein 1 (AP-1) family transcription factors at this regulatory element. dhs 37-40 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 219-223 10708521-3 2000 We previously characterized DHS at -79.5 and -20.9 kb with respect to the CFTR translational start site, DHS 3" to the gene at 4574 + 5.4-7.4 and 4574 + 15.6 kb, and a regulatory element in the first intron of the gene at 185 + 10 kb. dhs 28-31 CF transmembrane conductance regulator Homo sapiens 74-78 10708521-4 2000 We generated a cosmid contig to provide probes to evaluate the whole of the CFTR gene for DHS and have now mapped novel sites in introns 2, 3, 10, 16, 17a, 18, 20, and 21. dhs 90-93 CF transmembrane conductance regulator Homo sapiens 76-80 10561583-4 1999 We have now investigated further the DHS at -20.5 kb to evaluate its potential function in the regulation of CFTR expression. dhs 37-40 CF transmembrane conductance regulator Homo sapiens 109-113 10561583-10 1999 Thus, we provide evidence for a role for the -20.9 kb DHS in the transcriptional regulation of the CFTR gene, although the mechanisms mediating this effect remain unclear. dhs 54-57 CF transmembrane conductance regulator Homo sapiens 99-103