PMID-sentid	Pub_year	Sent_text	compound_name	comp_offset	prot_official_name	organism	prot_offset
8057282-4	1994	In the same system, the related oxysterol, 25-hydroxycholesterol (1), at 10 microM lowered both HMGR mRNA levels and LDL-receptor activity by 58% and 64%, respectively.	25-hydroxycholesterol	43-64	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	96-100
8057282-4	1994	In the same system, the related oxysterol, 25-hydroxycholesterol (1), at 10 microM lowered both HMGR mRNA levels and LDL-receptor activity by 58% and 64%, respectively.	25-hydroxycholesterol	43-64	low density lipoprotein receptor	Homo sapiens	117-129
8217873-5	1993	All cells studied herein possessed an oxysterol binding protein with high affinity for 25-hydroxycholesterol.	25-hydroxycholesterol	87-108	oxysterol binding protein	Homo sapiens	38-63
8305487-0	1994	Ketoconazole and 25-hydroxycholesterol produce reciprocal changes in the rate of transcription of the human LDL receptor gene.	25-hydroxycholesterol	17-38	low density lipoprotein receptor	Homo sapiens	108-120
8305487-5	1994	Ketoconazole- and 25-hydroxycholesterol-induced changes in LDL receptor mRNA accumulation were due to alterations in the relative rate of LDL receptor gene transcription as measured by nuclear run-on transcription.	25-hydroxycholesterol	18-39	low density lipoprotein receptor	Homo sapiens	59-71
8305487-5	1994	Ketoconazole- and 25-hydroxycholesterol-induced changes in LDL receptor mRNA accumulation were due to alterations in the relative rate of LDL receptor gene transcription as measured by nuclear run-on transcription.	25-hydroxycholesterol	18-39	low density lipoprotein receptor	Homo sapiens	138-150
7926656-2	1994	Insulin (0.4-40 micrograms/ml) had no effect on its own, but potentiated human chorionic gonadotropin (hCG) and 25-hydroxycholesterol-stimulated testosterone production by prematurational full-grown follicles in both a dose- and time-dependent manner.	25-hydroxycholesterol	112-133	insulin	Homo sapiens	0-7
7926656-6	1994	Insulin also enhanced conversion of 25-hydroxycholesterol to testosterone, but had no effect on the metabolism of pregnenolone, suggesting that insulin either enhances mobilization of cholesterol to the mitochondria or increases the activity of the cytochrome P450 side-chain cleavage enzyme.	25-hydroxycholesterol	36-57	insulin	Homo sapiens	0-7
7926656-6	1994	Insulin also enhanced conversion of 25-hydroxycholesterol to testosterone, but had no effect on the metabolism of pregnenolone, suggesting that insulin either enhances mobilization of cholesterol to the mitochondria or increases the activity of the cytochrome P450 side-chain cleavage enzyme.	25-hydroxycholesterol	36-57	insulin	Homo sapiens	144-151
7926656-7	1994	The ability of insulin to enhance hCG and/or 25-hydroxycholesterol-stimulated testosterone production was blocked by the addition of the protein kinase A (PKA) inhibitor H89, which confirmed that these actions of insulin are exerted, at least in part, by the cAMP/PKA pathway.	25-hydroxycholesterol	45-66	insulin	Homo sapiens	15-22
7926656-7	1994	The ability of insulin to enhance hCG and/or 25-hydroxycholesterol-stimulated testosterone production was blocked by the addition of the protein kinase A (PKA) inhibitor H89, which confirmed that these actions of insulin are exerted, at least in part, by the cAMP/PKA pathway.	25-hydroxycholesterol	45-66	insulin	Homo sapiens	213-220
8144506-7	1994	There was a slight induction of cholesterol 7 alpha-hydroxylase mRNA levels by 25-hydroxycholesterol at 8 h (+18%); but by 24 h, its level was below that of the control (-47%).	25-hydroxycholesterol	79-100	cytochrome P450 family 7 subfamily A member 1	Homo sapiens	32-63
7510705-6	1994	We found that bFGF and a protein kinase C (PKC) activator, phorbol 12-myristate 13-acetate, significantly induced luciferase activity driven by the LDL receptor promoter, whereas 25-hydroxycholesterol reduced the luciferase activity in bFGF-stimulated cells.	25-hydroxycholesterol	179-200	fibroblast growth factor 2	Homo sapiens	14-18
7510705-6	1994	We found that bFGF and a protein kinase C (PKC) activator, phorbol 12-myristate 13-acetate, significantly induced luciferase activity driven by the LDL receptor promoter, whereas 25-hydroxycholesterol reduced the luciferase activity in bFGF-stimulated cells.	25-hydroxycholesterol	179-200	fibroblast growth factor 2	Homo sapiens	236-240
8142309-5	1994	However, in neither line are the levels of oxysterol binding protein mRNA affected by 1 microM 25-hydroxycholesterol.	25-hydroxycholesterol	95-116	oxysterol binding protein	Homo sapiens	43-68
8142309-7	1994	The levels of CNBP mRNA are significantly reduced by 25-hydroxycholesterol in the sensitive CEM C7 cells, in which the dose response and time course are consistent with occupancy of the oxysterol binding protein by oxysterol and with subsequent cell kill.	25-hydroxycholesterol	53-74	CCHC-type zinc finger nucleic acid binding protein	Homo sapiens	14-18
8142309-7	1994	The levels of CNBP mRNA are significantly reduced by 25-hydroxycholesterol in the sensitive CEM C7 cells, in which the dose response and time course are consistent with occupancy of the oxysterol binding protein by oxysterol and with subsequent cell kill.	25-hydroxycholesterol	53-74	oxysterol binding protein	Homo sapiens	186-211
8217873-6	1993	For all clones grown in serum-free medium, the half-maximal cytolytic concentration of 25-hydroxycholesterol (20-40 nM) correlated with its affinity (Kd = approximately 31 nM) for this oxysterol binding protein.	25-hydroxycholesterol	87-108	oxysterol binding protein	Homo sapiens	185-210
8352528-6	1993	Following a 25-hydroxycholesterol treatment as short as 4 h, the onset of DNA synthesis was delayed, indicating that a certain level of HMG CoA reductase activity (= mevalonate synthesis) in the early and mid stage of the prereplicative phase is required for the transduction of the signal leading to initiation of DNA synthesis.	25-hydroxycholesterol	12-33	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	136-153
8344216-5	1993	IGF-II (100 ng/ml) enhanced progesterone biosynthesis approximately 2-fold in the presence of 25-hydroxycholesterol, suggesting that IGF-II increases the effective activity of the mitochondrial cholesterol side-chain cleavage enzyme.	25-hydroxycholesterol	94-115	insulin-like growth factor II	Sus scrofa	0-6
8344216-5	1993	IGF-II (100 ng/ml) enhanced progesterone biosynthesis approximately 2-fold in the presence of 25-hydroxycholesterol, suggesting that IGF-II increases the effective activity of the mitochondrial cholesterol side-chain cleavage enzyme.	25-hydroxycholesterol	94-115	insulin-like growth factor II	Sus scrofa	133-139
8352528-1	1993	Treatment with 25-hydroxycholesterol, an inhibitor of HMG CoA reductase activity, efficiently blocked the proliferation of both human normal mammary epithelial cells (HMEC) and breast cancer cells (MDA231).	25-hydroxycholesterol	15-36	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	54-71
8352528-5	1993	If 25-hydroxycholesterol was added along with insulin, the subsequent initiation of DNA synthesis was prevented.	25-hydroxycholesterol	3-24	insulin	Homo sapiens	46-53
8388388-7	1993	In contrast, 25-hydroxycholesterol strongly cosuppressed HMG-CoA reductase protein and mRNA levels and the low density lipoprotein receptor protein.	25-hydroxycholesterol	13-34	low density lipoprotein receptor	Homo sapiens	107-139
8490050-7	1993	The rate of side-chain cleavage of 25-hydroxycholesterol by human cytochrome P-450scc in Tween-20 micelles was low, the highest rate being about 1% of the Vmax for cholesterol.	25-hydroxycholesterol	35-56	cytochrome P450 family 11 subfamily A member 1	Homo sapiens	66-85
8490050-10	1993	Side-chain cleavage of 25-hydroxycholesterol by bovine cytochrome P-450scc showed similar characteristics to the human enzyme, except that the highest velocity observed was approx.	25-hydroxycholesterol	23-44	cholesterol side-chain cleavage enzyme, mitochondrial	Bos taurus	55-74
8463321-0	1993	Induction of basic fibroblast growth factor mRNA and protein synthesis in smooth muscle cells by cholesteryl ester enrichment and 25-hydroxycholesterol.	25-hydroxycholesterol	130-151	fibroblast growth factor 2	Homo sapiens	13-43
8463321-8	1993	25-Hydroxycholesterol also increases the release of bFGF-like mitogens from smooth muscle cells, as well as increasing mRNA transcript levels for bFGF.	25-hydroxycholesterol	0-21	fibroblast growth factor 2	Homo sapiens	52-56
8463321-8	1993	25-Hydroxycholesterol also increases the release of bFGF-like mitogens from smooth muscle cells, as well as increasing mRNA transcript levels for bFGF.	25-hydroxycholesterol	0-21	fibroblast growth factor 2	Homo sapiens	146-150
7685352-11	1993	The ratio between the level of HSS mRNA in cells grown in the absence and presence of 5 micrograms/ml 25-hydroxycholesterol varies between 8- and 16-fold.	25-hydroxycholesterol	102-123	growth factor, augmenter of liver regeneration	Homo sapiens	31-34
7685352-14	1993	These studies show that HSS exhibit a relatively high level of transcriptional regulation in response to 25-hydroxycholesterol regardless of the presence of cholesterol in the growth media.	25-hydroxycholesterol	105-126	growth factor, augmenter of liver regeneration	Homo sapiens	24-27
8387332-8	1993	As assessed by immunoblots and steady-state labeling of proteins followed by immunoprecipitation of the LDL receptor, cells incubated with micellar 25-hydroxycholesterol contained substantially less receptor protein.	25-hydroxycholesterol	148-169	low density lipoprotein receptor	Homo sapiens	104-116
1525044-4	1992	The role of different steroids in the regulation of P450 cholesterol side-chain cleavage enzyme (P450scc) and 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) was evaluated by measuring the conversion of P4 derived from unlabelled 25-hydroxycholesterol and from labelled pregnenolone, respectively.	25-hydroxycholesterol	231-252	cytochrome P450 family 11 subfamily A member 1	Homo sapiens	97-104
1525044-4	1992	The role of different steroids in the regulation of P450 cholesterol side-chain cleavage enzyme (P450scc) and 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) was evaluated by measuring the conversion of P4 derived from unlabelled 25-hydroxycholesterol and from labelled pregnenolone, respectively.	25-hydroxycholesterol	231-252	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1	Homo sapiens	147-157
1329783-0	1992	The effect of 25-hydroxycholesterol on the regulation of apolipoprotein E mRNA levels and secretion in the human hepatoma HepG2.	25-hydroxycholesterol	14-35	apolipoprotein E	Homo sapiens	57-73
1352965-1	1992	25-Hydroxycholesterol regulates cholesterol biosynthesis by two mechanisms: repression of the transcription of the genes for several cholesterogenic enzymes and acceleration of the degradation of the enzyme 3-hydroxy-3-methylglutaryl CoA reductase.	25-hydroxycholesterol	0-21	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	207-247
1325152-9	1992	Under all circumstances levels of 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD), as assessed by Northern blotting or by conversion of 25-hydroxycholesterol, were very low.	25-hydroxycholesterol	137-158	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1	Homo sapiens	71-81
2065103-4	1991	Activation of acyl-CoA cholesterol acyltransferase (ACAT) was greater than 100-fold in both control and NP-C fibroblasts when cell cultures were preconditioned with 25-hydroxycholesterol, but the subsequent esterification of exogenous non-lipoprotein [3H]cholesterol remained deficient in all NP-C cells.	25-hydroxycholesterol	165-186	sterol O-acyltransferase 1	Homo sapiens	14-50
1712015-5	1991	The importance of HMG-CoA reductase induction and mevalonate production in cell cycle progression was demonstrated by the observation that either 25-hydroxycholesterol, which inhibits this induction, or lovastatin, a competitive inhibitor of HMG-CoA reductase, inhibited anti-CD3-induced T cell mitogenesis in a dose-dependent manner.	25-hydroxycholesterol	146-167	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	18-35
1712015-5	1991	The importance of HMG-CoA reductase induction and mevalonate production in cell cycle progression was demonstrated by the observation that either 25-hydroxycholesterol, which inhibits this induction, or lovastatin, a competitive inhibitor of HMG-CoA reductase, inhibited anti-CD3-induced T cell mitogenesis in a dose-dependent manner.	25-hydroxycholesterol	146-167	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	242-259
1346397-6	1992	Surprisingly, the addition of A23187 to THP-1 cells incubated in the presence of 25-hydroxycholesterol and mevalonic acid also led to significant increases in the mRNA levels for HMG-CoA reductase and HMG-CoA synthase.	25-hydroxycholesterol	81-102	GLI family zinc finger 2	Homo sapiens	40-45
1346397-6	1992	Surprisingly, the addition of A23187 to THP-1 cells incubated in the presence of 25-hydroxycholesterol and mevalonic acid also led to significant increases in the mRNA levels for HMG-CoA reductase and HMG-CoA synthase.	25-hydroxycholesterol	81-102	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	179-196
1346397-6	1992	Surprisingly, the addition of A23187 to THP-1 cells incubated in the presence of 25-hydroxycholesterol and mevalonic acid also led to significant increases in the mRNA levels for HMG-CoA reductase and HMG-CoA synthase.	25-hydroxycholesterol	81-102	3-hydroxy-3-methylglutaryl-CoA synthase 2	Homo sapiens	201-217
1546367-0	1992	Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase synthesis in Syrian hamster C100 cells by mevinolin, 25-hydroxycholesterol, and mevalonate: the role of posttranscriptional control.	25-hydroxycholesterol	115-136	3-hydroxy-3-methylglutaryl-coenzyme A reductase	Mesocricetus auratus	14-61
1546367-3	1992	The addition of 25-hydroxycholesterol to the cell culture medium resulted in a fourfold decrease in both the rate of synthesis and mRNA level for HMG-CoA reductase.	25-hydroxycholesterol	16-37	3-hydroxy-3-methylglutaryl-coenzyme A reductase	Mesocricetus auratus	146-163
1546367-6	1992	Degradation of HMG-CoA reductase was rapid in the presence (t1/2 = 1.34 h) or absence (t1/2 = 1.17 h) of mevinolin and was not changed significantly by adding either 25-hydroxycholesterol, alone (t1/2 = 1.30 h) or both 25-hydroxycholesterol and mevalonate (t1/2 = 1.30 h) to mevinolin-treated cells.	25-hydroxycholesterol	166-187	3-hydroxy-3-methylglutaryl-coenzyme A reductase	Mesocricetus auratus	15-32
1546367-6	1992	Degradation of HMG-CoA reductase was rapid in the presence (t1/2 = 1.34 h) or absence (t1/2 = 1.17 h) of mevinolin and was not changed significantly by adding either 25-hydroxycholesterol, alone (t1/2 = 1.30 h) or both 25-hydroxycholesterol and mevalonate (t1/2 = 1.30 h) to mevinolin-treated cells.	25-hydroxycholesterol	219-240	3-hydroxy-3-methylglutaryl-coenzyme A reductase	Mesocricetus auratus	15-32
1546367-7	1992	This study demonstrates that mevalonate and 25-hydroxycholesterol act synergistically in the presence of mevinolin to achieve a greater degree of suppression in the rate of HMG-CoA reductase synthesis than can be accounted for by their individual effects on HMG-CoA reductase mRNA.	25-hydroxycholesterol	44-65	3-hydroxy-3-methylglutaryl-coenzyme A reductase	Mesocricetus auratus	173-190
1546367-7	1992	This study demonstrates that mevalonate and 25-hydroxycholesterol act synergistically in the presence of mevinolin to achieve a greater degree of suppression in the rate of HMG-CoA reductase synthesis than can be accounted for by their individual effects on HMG-CoA reductase mRNA.	25-hydroxycholesterol	44-65	3-hydroxy-3-methylglutaryl-coenzyme A reductase	Mesocricetus auratus	258-275
2065103-4	1991	Activation of acyl-CoA cholesterol acyltransferase (ACAT) was greater than 100-fold in both control and NP-C fibroblasts when cell cultures were preconditioned with 25-hydroxycholesterol, but the subsequent esterification of exogenous non-lipoprotein [3H]cholesterol remained deficient in all NP-C cells.	25-hydroxycholesterol	165-186	sterol O-acyltransferase 1	Homo sapiens	52-56
1716067-7	1991	Mediation of binding by the LDL receptor is demonstrated by correspondence between the LDL receptor dissociation constant derived from this work and literature values; increased specific binding in lymphocytes cultured in lipoprotein-deficient media to up-regulate the LDL receptor; and decreased specific binding in lymphocytes cultured in the presence of 25-hydroxy cholesterol for 48 h to suppress the LDL receptor.	25-hydroxycholesterol	357-379	low density lipoprotein receptor	Homo sapiens	28-40
2004596-6	1991	IGF-I increased functional enzymatic activity, observed as increased progesterone accumulation in the presence of 25-hydroxycholesterol used as exogenous substrate.	25-hydroxycholesterol	114-135	insulin-like growth factor I	Oryctolagus cuniculus	0-5
2005896-0	1991	Activation of the silent endogenous cholesterol-7-alpha-hydroxylase gene in rat hepatoma cells: a new complementation group having resistance to 25-hydroxycholesterol.	25-hydroxycholesterol	145-166	cytochrome P450 family 7 subfamily A member 1	Rattus norvegicus	36-67
2066678-5	1991	In addition, we observed that the apoE gene responded more promptly to 25-hydroxycholesterol than to exogenous cholesterol.	25-hydroxycholesterol	71-92	apolipoprotein E	Homo sapiens	34-38
2023060-3	1991	Some cholesterol oxides, notably cholestane-3 beta, 5 alpha, 6 beta-triol and 25-hydroxycholesterol, have been shown to cause injury to vascular endothelial and smooth muscle cells, to alter LDL receptor function, to enhance cholesteryl ester accumulation, to inhibit prostacyclin production, and to induce experimental atherosclerosis alone or in combination with cholesterol.	25-hydroxycholesterol	78-99	low density lipoprotein receptor	Homo sapiens	191-203
2090710-11	1990	Down-regulation of LDL receptor activity occurred when infected WHHL cells were preincubated with either LDL or cholesterol plus 25-hydroxycholesterol.	25-hydroxycholesterol	129-150	low-density lipoprotein receptor	Cricetulus griseus	19-31
2176219-7	1990	Exposure of cells to mevinolin and 25-hydroxycholesterol together resulted in a marked repression of HMG-CoA reductase mRNA levels, whereas these conditions further enhanced the secretion of H-TGL activity and the expression of H-TGL mRNA.	25-hydroxycholesterol	35-56	lipase C, hepatic type	Homo sapiens	191-196
2176219-7	1990	Exposure of cells to mevinolin and 25-hydroxycholesterol together resulted in a marked repression of HMG-CoA reductase mRNA levels, whereas these conditions further enhanced the secretion of H-TGL activity and the expression of H-TGL mRNA.	25-hydroxycholesterol	35-56	lipase C, hepatic type	Homo sapiens	228-233
2176219-8	1990	These results demonstrate a differential role for 25-hydroxycholesterol in the regulation of H-TGL and HMG-CoA reductase expression.	25-hydroxycholesterol	50-71	lipase C, hepatic type	Homo sapiens	93-98
2226300-8	1990	In contrast, incubation of cytotrophoblasts with IGF-II for 24 h significantly increased the 3 beta HSD activity (as determined by the conversion of pregnenolone to progesterone) and P450scc activity (as determined by the conversion of 25-hydroxycholesterol to progesterone) of these cells.	25-hydroxycholesterol	236-257	insulin like growth factor 2	Homo sapiens	49-55
2086705-10	1990	By contrast, the oxygenated sterol, 25-hydroxycholesterol, and mevalonate, the precursor of endogenously synthesized sterols, down-regulated LDL receptor mRNA levels comparably in mitogen-stimulated and control PBMC.	25-hydroxycholesterol	36-57	low density lipoprotein receptor	Homo sapiens	141-153
2078637-4	1990	In contrast, progesterone, an ACAT activity inhibitor, used at 5-30 M diminished the rate of FC esterification, when MPM were incubated with acetyl-LDL of 25-hydroxycholesterol.	25-hydroxycholesterol	155-176	sterol O-acyltransferase 2	Mus musculus	30-34
34953867-4	2022	25-Hydroxycholesterol has been shown to be synthesized by macrophages in response to the activation of Toll-like receptors and to offer protection against microbial pathogens, while 7alpha,25-dihydroxycholesterol has been shown to act as a chemoattractant to lymphocytes expressing the G protein-coupled receptor EBI2 and to be important in coordinating the action of B, T, and dendritic cells in secondary lymphoid tissue.	25-hydroxycholesterol	0-21	G protein-coupled receptor 183	Homo sapiens	313-317
2109690-9	1990	Exogenous substrate added to whole follicles cultured in the presence or absence of TNF indicated that TNF enhanced the conversion of 25-hydroxycholesterol to pregnenolone.	25-hydroxycholesterol	134-155	tumor necrosis factor	Rattus norvegicus	103-106
2180958-4	1990	Coincubations with 25-hydroxycholesterol markedly attenuated fibroblast LDL receptor protein and mRNA response to growth activation.	25-hydroxycholesterol	19-40	low density lipoprotein receptor	Homo sapiens	72-84
7925343-4	1994	After transfection with cDNA for human cholesterol 7 alpha-hydroxylase, COS cells showed a significant activity towards cholesterol but not towards 25-hydroxycholesterol.	25-hydroxycholesterol	148-169	cytochrome P450 family 7 subfamily A member 1	Homo sapiens	39-70
7925343-5	1994	During purification of cholesterol 7 alpha-hydroxylase from pig liver microsomes, about 99% of the 7 alpha-hydroxylase activity towards 25-hydroxycholesterol and 27-hydroxycholesterol was clearly separated from 7 alpha-hydroxylase activity for cholesterol.	25-hydroxycholesterol	136-157	cytochrome P450 family 7 subfamily A member 1	Sus scrofa	23-54
7925343-9	1994	It has been suggested that cholesterol 7 alpha-hydroxylase can preferentially use oxysterols, in particular 25-hydroxycholesterol, as substrates and by this means inactivate important physiological regulators of cholesterol homeostasis.	25-hydroxycholesterol	108-129	cytochrome P450 family 7 subfamily A member 1	Sus scrofa	27-58
34808332-0	2022	25-Hydroxycholesterol suppress IFN-gamma-induced inflammation in microglia by disrupting lipid raft formation and caveolin-mediated signaling endosomes.	25-hydroxycholesterol	0-21	interferon gamma	Homo sapiens	31-40
34808332-8	2022	25-HC repressed IFN-gamma induction of Cav-1 expression in microglia, and subsequently suppressed the chronic inflammatory response.	25-hydroxycholesterol	0-5	interferon gamma	Homo sapiens	16-25
34808332-8	2022	25-HC repressed IFN-gamma induction of Cav-1 expression in microglia, and subsequently suppressed the chronic inflammatory response.	25-hydroxycholesterol	0-5	caveolin 1	Homo sapiens	39-44
34861388-0	2022	25-hydroxycholesterol-induced cell death via activation of ROCK/LIMK/cofilin axis in colorectal cancer cell spheroids.	25-hydroxycholesterol	0-21	LIM domain kinase 1	Homo sapiens	64-68
34861388-0	2022	25-hydroxycholesterol-induced cell death via activation of ROCK/LIMK/cofilin axis in colorectal cancer cell spheroids.	25-hydroxycholesterol	0-21	cofilin 1	Homo sapiens	69-76
2117604-9	1990	25-Hydroxycholesterol is a poor regulator of the synthesis and degradation of the rate-limiting enzyme, 3-hydroxy-3-methylglutaryl-coenzyme A reductase in crB in comparison to the wild-type Chinese hamster ovary K1 cells.	25-hydroxycholesterol	0-21	3-hydroxy-3-methylglutaryl-coenzyme A reductase	Cricetulus griseus	104-151
34090999-7	2021	Use of the anti-hypertensive drug, reserpine, mimicked the effects of the CH25H product 25-hydroxycholesterol to suppress TEV uptake and TEV-mediated tumor growth, pre-metastatic niche formation, and lung metastasis.	25-hydroxycholesterol	88-109	cholesterol 25-hydroxylase	Mus musculus	74-79
34725187-11	2021	Effects of LPS on both synaptic plasticity and one-trial inhibitory avoidance learning are eliminated in mice deficient in Ch25h, the primary enzyme responsible for endogenous 25HC synthesis.	25-hydroxycholesterol	176-180	cholesterol 25-hydroxylase	Mus musculus	123-128
34781692-9	2021	We also found that the gene encoding cholesterol 25-hydroxylase (CH25H), which converts cholesterol to 25HC, can be induced by type I interferon (IFN) in human B cell-enriched peripheral blood mononuclear cells (PBMCs).	25-hydroxycholesterol	103-107	cholesterol 25-hydroxylase	Homo sapiens	37-63
34781692-9	2021	We also found that the gene encoding cholesterol 25-hydroxylase (CH25H), which converts cholesterol to 25HC, can be induced by type I interferon (IFN) in human B cell-enriched peripheral blood mononuclear cells (PBMCs).	25-hydroxycholesterol	103-107	cholesterol 25-hydroxylase	Homo sapiens	65-70
34323663-13	2021	Rivaroxaban 100 mg/ml+25-OHC increased the VE-cadherin expression in endothelium as compared to 25-OHC (p < .05).	25-hydroxycholesterol	22-28	cadherin 5	Homo sapiens	43-54
34644558-0	2021	The cholesterol metabolite 25-hydroxycholesterol restrains the transcriptional regulator SREBP2 and limits intestinal IgA plasma cell differentiation.	25-hydroxycholesterol	27-48	sterol regulatory element binding factor 2	Mus musculus	89-95
34323663-10	2021	RESULTS: 25-OHC decreased endothelial cell integrity and increased the mRNA expression of IL-33, tissue factor, ICAM-1, MCP-1, VEGF, TNF-alpha as compared to unstimulated controls.	25-hydroxycholesterol	9-15	interleukin 33	Homo sapiens	90-95
34323663-10	2021	RESULTS: 25-OHC decreased endothelial cell integrity and increased the mRNA expression of IL-33, tissue factor, ICAM-1, MCP-1, VEGF, TNF-alpha as compared to unstimulated controls.	25-hydroxycholesterol	9-15	coagulation factor III, tissue factor	Homo sapiens	97-110
34323663-10	2021	RESULTS: 25-OHC decreased endothelial cell integrity and increased the mRNA expression of IL-33, tissue factor, ICAM-1, MCP-1, VEGF, TNF-alpha as compared to unstimulated controls.	25-hydroxycholesterol	9-15	intercellular adhesion molecule 1	Homo sapiens	112-118
34323663-10	2021	RESULTS: 25-OHC decreased endothelial cell integrity and increased the mRNA expression of IL-33, tissue factor, ICAM-1, MCP-1, VEGF, TNF-alpha as compared to unstimulated controls.	25-hydroxycholesterol	9-15	C-C motif chemokine ligand 2	Homo sapiens	120-125
34323663-10	2021	RESULTS: 25-OHC decreased endothelial cell integrity and increased the mRNA expression of IL-33, tissue factor, ICAM-1, MCP-1, VEGF, TNF-alpha as compared to unstimulated controls.	25-hydroxycholesterol	9-15	vascular endothelial growth factor A	Homo sapiens	127-131
34323663-10	2021	RESULTS: 25-OHC decreased endothelial cell integrity and increased the mRNA expression of IL-33, tissue factor, ICAM-1, MCP-1, VEGF, TNF-alpha as compared to unstimulated controls.	25-hydroxycholesterol	9-15	tumor necrosis factor	Homo sapiens	133-142
34391813-11	2021	The same action had inhibition of estrogen receptor alpha, Gi-protein, Gbetagamma, phospholipase C and protein kinase C. Additionally, 1muM 25HC upregulated ROS production in a Ca2+-dependent way and an antioxidant partially decreased the exocytosis-promoting effect of 25HC.	25-hydroxycholesterol	140-144	estrogen receptor 1 (alpha)	Mus musculus	34-57
34492054-6	2021	Pre-incubation with 25-OHC resulted in decreased endothelial cell integrity (p<0.001), decreased expression of ZO-1 mRNA (p<0.05) and protein levels (p<0.05), OCLN mRNA (p<0.05) and protein levels (p<0.05) and increased ICAM-1 mRNA (p<0.001) and protein levels (p<0.001) compared to the control group.	25-hydroxycholesterol	20-26	tight junction protein 1	Homo sapiens	111-115
34492054-6	2021	Pre-incubation with 25-OHC resulted in decreased endothelial cell integrity (p<0.001), decreased expression of ZO-1 mRNA (p<0.05) and protein levels (p<0.05), OCLN mRNA (p<0.05) and protein levels (p<0.05) and increased ICAM-1 mRNA (p<0.001) and protein levels (p<0.001) compared to the control group.	25-hydroxycholesterol	20-26	occludin	Homo sapiens	159-163
34492054-6	2021	Pre-incubation with 25-OHC resulted in decreased endothelial cell integrity (p<0.001), decreased expression of ZO-1 mRNA (p<0.05) and protein levels (p<0.05), OCLN mRNA (p<0.05) and protein levels (p<0.05) and increased ICAM-1 mRNA (p<0.001) and protein levels (p<0.001) compared to the control group.	25-hydroxycholesterol	20-26	intercellular adhesion molecule 1	Homo sapiens	220-226
34769299-2	2021	The enzymatic product of cholesterol 25-hydroxylase (CH25H), 25-Hydroxycholesterol (25-HC), was reported to have potent anti-SARS-CoV-2 activity.	25-hydroxycholesterol	61-82	cholesterol 25-hydroxylase	Homo sapiens	25-51
34769299-2	2021	The enzymatic product of cholesterol 25-hydroxylase (CH25H), 25-Hydroxycholesterol (25-HC), was reported to have potent anti-SARS-CoV-2 activity.	25-hydroxycholesterol	61-82	cholesterol 25-hydroxylase	Homo sapiens	53-58
34769299-2	2021	The enzymatic product of cholesterol 25-hydroxylase (CH25H), 25-Hydroxycholesterol (25-HC), was reported to have potent anti-SARS-CoV-2 activity.	25-hydroxycholesterol	84-89	cholesterol 25-hydroxylase	Homo sapiens	25-51
34769299-2	2021	The enzymatic product of cholesterol 25-hydroxylase (CH25H), 25-Hydroxycholesterol (25-HC), was reported to have potent anti-SARS-CoV-2 activity.	25-hydroxycholesterol	84-89	cholesterol 25-hydroxylase	Homo sapiens	53-58
34061318-0	2021	Cholesterol-25-Hydroxylase Suppresses Seneca Valley Virus Infection via Producing 25-Hydroxycholesterol to Block Adsorption Procedure.	25-hydroxycholesterol	82-103	cholesterol 25-hydroxylase	Homo sapiens	0-26
34061318-2	2021	CH25H catalyzes cholesterol to produce 25-hydroxycholesterol (25HC) by adding a second hydroxyl to the 25th carbon atom of cholesterol.	25-hydroxycholesterol	39-60	cholesterol 25-hydroxylase	Homo sapiens	0-5
34061318-2	2021	CH25H catalyzes cholesterol to produce 25-hydroxycholesterol (25HC) by adding a second hydroxyl to the 25th carbon atom of cholesterol.	25-hydroxycholesterol	62-66	cholesterol 25-hydroxylase	Homo sapiens	0-5
34061318-8	2021	This study reveals that CH25H exerts the advantage of innate immunity mainly by producing 25HC to block virion adsorption.	25-hydroxycholesterol	90-94	cholesterol 25-hydroxylase	Homo sapiens	24-29
34551004-0	2021	Tumor Necrosis Factor-alpha utilizes MAPK/NFkappaB pathways to induce cholesterol-25 hydroxylase for amplifying pro-inflammatory response via 25-hydroxycholesterol-integrin-FAK pathway.	25-hydroxycholesterol	142-163	tumor necrosis factor	Homo sapiens	0-27
34551004-0	2021	Tumor Necrosis Factor-alpha utilizes MAPK/NFkappaB pathways to induce cholesterol-25 hydroxylase for amplifying pro-inflammatory response via 25-hydroxycholesterol-integrin-FAK pathway.	25-hydroxycholesterol	142-163	cholesterol 25-hydroxylase	Homo sapiens	70-96
34551004-0	2021	Tumor Necrosis Factor-alpha utilizes MAPK/NFkappaB pathways to induce cholesterol-25 hydroxylase for amplifying pro-inflammatory response via 25-hydroxycholesterol-integrin-FAK pathway.	25-hydroxycholesterol	142-163	protein tyrosine kinase 2	Homo sapiens	173-176
34551004-5	2021	In the current study, we have identified an oxysterol 25-hydroxycholesterol (25HC) as a soluble extracellular lipid amplifying TNF mediated innate immune pro-inflammatory response.	25-hydroxycholesterol	77-81	tumor necrosis factor	Homo sapiens	127-130
35537452-3	2022	Here, we report the cryo-EM structures of an EBI2-Gi signaling complex with its endogenous agonist 7alpha,25-OHC and that of an inactive EBI2 bound to the inverse agonist GSK682753A.	25-hydroxycholesterol	106-112	G protein-coupled receptor 183	Homo sapiens	45-49
34406977-0	2021	25-Hydroxycholesterol protecting from cerebral ischemia-reperfusion injury through the inhibition of STING activity.	25-hydroxycholesterol	0-21	stimulator of interferon response cGAMP interactor 1	Mus musculus	101-106
34406977-8	2021	Thus, 25-HC improved MCAO injury through the STING channel.	25-hydroxycholesterol	6-11	stimulator of interferon response cGAMP interactor 1	Mus musculus	45-50
34298014-9	2021	Furthermore, ATF4 proactively upregulated the cell death inducible genes expression, such as Chop, Chac1, and Trb3, thereby markedly reducing cell viability with 25-hydroxycholesterol.	25-hydroxycholesterol	162-183	activating transcription factor 4	Homo sapiens	13-17
34298014-9	2021	Furthermore, ATF4 proactively upregulated the cell death inducible genes expression, such as Chop, Chac1, and Trb3, thereby markedly reducing cell viability with 25-hydroxycholesterol.	25-hydroxycholesterol	162-183	DNA damage inducible transcript 3	Homo sapiens	93-97
34298014-9	2021	Furthermore, ATF4 proactively upregulated the cell death inducible genes expression, such as Chop, Chac1, and Trb3, thereby markedly reducing cell viability with 25-hydroxycholesterol.	25-hydroxycholesterol	162-183	ChaC glutathione specific gamma-glutamylcyclotransferase 1	Homo sapiens	99-104
34298014-9	2021	Furthermore, ATF4 proactively upregulated the cell death inducible genes expression, such as Chop, Chac1, and Trb3, thereby markedly reducing cell viability with 25-hydroxycholesterol.	25-hydroxycholesterol	162-183	tribbles pseudokinase 3	Homo sapiens	110-114
35587189-4	2022	Cholesterol-25-hydroxylase (CH25H) and its enzymatic product, 25-hydroxycholesterol (25HC), were previously shown to exhibit effective broad-spectrum antiviral activity.	25-hydroxycholesterol	62-83	cholesterol 25-hydroxylase	Homo sapiens	0-26
35587189-4	2022	Cholesterol-25-hydroxylase (CH25H) and its enzymatic product, 25-hydroxycholesterol (25HC), were previously shown to exhibit effective broad-spectrum antiviral activity.	25-hydroxycholesterol	62-83	cholesterol 25-hydroxylase	Homo sapiens	28-33
35587189-4	2022	Cholesterol-25-hydroxylase (CH25H) and its enzymatic product, 25-hydroxycholesterol (25HC), were previously shown to exhibit effective broad-spectrum antiviral activity.	25-hydroxycholesterol	85-89	cholesterol 25-hydroxylase	Homo sapiens	0-26
35587189-4	2022	Cholesterol-25-hydroxylase (CH25H) and its enzymatic product, 25-hydroxycholesterol (25HC), were previously shown to exhibit effective broad-spectrum antiviral activity.	25-hydroxycholesterol	85-89	cholesterol 25-hydroxylase	Homo sapiens	28-33
35587189-14	2022	IMPORTANCE The enzymatic product of CH25H, 25-hydroxycholesterol (25HC), has been previously shown to play a critical role in the blockage of the cell-virus fusion in response to viral infection.	25-hydroxycholesterol	43-64	cholesterol 25-hydroxylase	Homo sapiens	36-41
35587189-14	2022	IMPORTANCE The enzymatic product of CH25H, 25-hydroxycholesterol (25HC), has been previously shown to play a critical role in the blockage of the cell-virus fusion in response to viral infection.	25-hydroxycholesterol	66-70	cholesterol 25-hydroxylase	Homo sapiens	36-41
35534729-4	2022	Notably, 25-hydroxycholesterol blocks ammonia to access its binding site on SCAP.	25-hydroxycholesterol	9-30	SREBF chaperone	Homo sapiens	76-80
34287319-5	2021	Indeed, by inducing CH25H, which catalyzes the formation of 25-hydroxycholesterol from cholesterol, NTZ treatment repressed cholesterol biosynthetic pathways and promoted cholesterol mobilization and efflux from the cell.	25-hydroxycholesterol	60-81	cholesterol 25-hydroxylase	Homo sapiens	20-25
35577580-7	2022	The results showed that 25-OHC decreased TGF-beta and IL-37 mRNA expression and increased EBI3, p35, IL-18, IL-23 mRNA expression in endothelial cell as compared to an untreated control (p<0.05).	25-hydroxycholesterol	24-30	transforming growth factor alpha	Homo sapiens	41-49
35577580-7	2022	The results showed that 25-OHC decreased TGF-beta and IL-37 mRNA expression and increased EBI3, p35, IL-18, IL-23 mRNA expression in endothelial cell as compared to an untreated control (p<0.05).	25-hydroxycholesterol	24-30	interleukin 37	Homo sapiens	54-59
35577580-7	2022	The results showed that 25-OHC decreased TGF-beta and IL-37 mRNA expression and increased EBI3, p35, IL-18, IL-23 mRNA expression in endothelial cell as compared to an untreated control (p<0.05).	25-hydroxycholesterol	24-30	Epstein-Barr virus induced 3	Homo sapiens	90-94
35577580-7	2022	The results showed that 25-OHC decreased TGF-beta and IL-37 mRNA expression and increased EBI3, p35, IL-18, IL-23 mRNA expression in endothelial cell as compared to an untreated control (p<0.05).	25-hydroxycholesterol	24-30	interleukin 12A	Homo sapiens	96-99
35577580-7	2022	The results showed that 25-OHC decreased TGF-beta and IL-37 mRNA expression and increased EBI3, p35, IL-18, IL-23 mRNA expression in endothelial cell as compared to an untreated control (p<0.05).	25-hydroxycholesterol	24-30	interleukin 18	Homo sapiens	101-106
35577580-7	2022	The results showed that 25-OHC decreased TGF-beta and IL-37 mRNA expression and increased EBI3, p35, IL-18, IL-23 mRNA expression in endothelial cell as compared to an untreated control (p<0.05).	25-hydroxycholesterol	24-30	interleukin 23 subunit alpha	Homo sapiens	108-113
35121342-9	2022	Pre-stimulation of HUVECs with 25-OHC decreased endothelial cell integrity (p < 0.001) and increased the expression of IL-33, ICAM-1, MCP-1, VEGF, TNF-alpha mRNA (p < 0.01) compared to unstimulated controls.	25-hydroxycholesterol	31-37	interleukin 33	Homo sapiens	119-124
35462473-2	2022	Cholesterol 25-hydroxylase (Ch25h) and its product, 25-hydroxycholesterol (25-HC), play important roles in cholesterol homeostasis and inflammation, but whether Ch25h and 25-HC are involved in NAFLD remains uncertain.	25-hydroxycholesterol	52-73	cholesterol 25-hydroxylase	Mus musculus	0-26
35462473-2	2022	Cholesterol 25-hydroxylase (Ch25h) and its product, 25-hydroxycholesterol (25-HC), play important roles in cholesterol homeostasis and inflammation, but whether Ch25h and 25-HC are involved in NAFLD remains uncertain.	25-hydroxycholesterol	52-73	cholesterol 25-hydroxylase	Mus musculus	28-33
35462473-2	2022	Cholesterol 25-hydroxylase (Ch25h) and its product, 25-hydroxycholesterol (25-HC), play important roles in cholesterol homeostasis and inflammation, but whether Ch25h and 25-HC are involved in NAFLD remains uncertain.	25-hydroxycholesterol	75-80	cholesterol 25-hydroxylase	Mus musculus	0-26
35462473-2	2022	Cholesterol 25-hydroxylase (Ch25h) and its product, 25-hydroxycholesterol (25-HC), play important roles in cholesterol homeostasis and inflammation, but whether Ch25h and 25-HC are involved in NAFLD remains uncertain.	25-hydroxycholesterol	75-80	cholesterol 25-hydroxylase	Mus musculus	28-33
35121342-9	2022	Pre-stimulation of HUVECs with 25-OHC decreased endothelial cell integrity (p < 0.001) and increased the expression of IL-33, ICAM-1, MCP-1, VEGF, TNF-alpha mRNA (p < 0.01) compared to unstimulated controls.	25-hydroxycholesterol	31-37	intercellular adhesion molecule 1	Homo sapiens	126-132
35121342-9	2022	Pre-stimulation of HUVECs with 25-OHC decreased endothelial cell integrity (p < 0.001) and increased the expression of IL-33, ICAM-1, MCP-1, VEGF, TNF-alpha mRNA (p < 0.01) compared to unstimulated controls.	25-hydroxycholesterol	31-37	C-C motif chemokine ligand 2	Homo sapiens	134-139
35121342-9	2022	Pre-stimulation of HUVECs with 25-OHC decreased endothelial cell integrity (p < 0.001) and increased the expression of IL-33, ICAM-1, MCP-1, VEGF, TNF-alpha mRNA (p < 0.01) compared to unstimulated controls.	25-hydroxycholesterol	31-37	vascular endothelial growth factor A	Homo sapiens	141-145
35121342-9	2022	Pre-stimulation of HUVECs with 25-OHC decreased endothelial cell integrity (p < 0.001) and increased the expression of IL-33, ICAM-1, MCP-1, VEGF, TNF-alpha mRNA (p < 0.01) compared to unstimulated controls.	25-hydroxycholesterol	31-37	tumor necrosis factor	Homo sapiens	147-156
35121342-12	2022	Dabigatran 500 mg/ml+ 25-OHC increased the endothelial expression of VE-cadherin as compared to 25-OHC (p < 0.01).	25-hydroxycholesterol	22-28	cadherin 5	Homo sapiens	69-80
35159129-6	2022	The effects of the hit compounds identified by molecular docking were compared in vitro with 25-hydroxycholesterol (25-HC), which is known to act as a PC for NP-C1.	25-hydroxycholesterol	93-114	NPC intracellular cholesterol transporter 1	Homo sapiens	158-163
35198901-0	2022	Senolysis induced by 25-hydroxycholesterol targets CRYAB in multiple cell types.	25-hydroxycholesterol	21-42	crystallin, alpha B	Mus musculus	51-56
35198901-4	2022	Using chemical inhibitor screening for CRYAB disruption, we identified 25-hydroxycholesterol (25HC), an endogenous metabolite of cholesterol biosynthesis, as a potent senolytic.	25-hydroxycholesterol	71-92	crystallin, alpha B	Mus musculus	39-44
35198901-4	2022	Using chemical inhibitor screening for CRYAB disruption, we identified 25-hydroxycholesterol (25HC), an endogenous metabolite of cholesterol biosynthesis, as a potent senolytic.	25-hydroxycholesterol	94-98	crystallin, alpha B	Mus musculus	39-44
35159303-5	2022	B1 cells expressed GPR183 at the mRNA level and migrated towards the GPR183 ligand 7alpha,25-dihydroxycholesterol (7alpha,25-OHC).	25-hydroxycholesterol	122-128	G protein-coupled receptor 183	Homo sapiens	69-75
34990887-1	2022	BACKGROUND & AIMS: Cholesterol 25-hydroxylase (Ch25h), converting cholesterol to 25-hydroxycholesterol (25-HC), is critical in modulating cellular lipid metabolism and anti-inflammatory and antiviral activities.	25-hydroxycholesterol	81-102	cholesterol 25-hydroxylase	Mus musculus	19-45
34990887-1	2022	BACKGROUND & AIMS: Cholesterol 25-hydroxylase (Ch25h), converting cholesterol to 25-hydroxycholesterol (25-HC), is critical in modulating cellular lipid metabolism and anti-inflammatory and antiviral activities.	25-hydroxycholesterol	81-102	cholesterol 25-hydroxylase	Mus musculus	47-52
34990887-1	2022	BACKGROUND & AIMS: Cholesterol 25-hydroxylase (Ch25h), converting cholesterol to 25-hydroxycholesterol (25-HC), is critical in modulating cellular lipid metabolism and anti-inflammatory and antiviral activities.	25-hydroxycholesterol	104-109	cholesterol 25-hydroxylase	Mus musculus	19-45
34990887-1	2022	BACKGROUND & AIMS: Cholesterol 25-hydroxylase (Ch25h), converting cholesterol to 25-hydroxycholesterol (25-HC), is critical in modulating cellular lipid metabolism and anti-inflammatory and antiviral activities.	25-hydroxycholesterol	104-109	cholesterol 25-hydroxylase	Mus musculus	47-52
3223954-3	1988	Stimulation of membrane ACAT activity by 25-hydroxycholesterol increased the synthesis of cholesteryl 12-HETE by 40%.	25-hydroxycholesterol	41-62	sterol O-acyltransferase 1	Homo sapiens	24-28
2590161-4	1989	Addition of 25-hydroxycholesterol to fibroblasts cultured in delipidated serum increased ACAT activity for all three cell types, although stimulation in NPD cells was less than that observed in NPC cells.	25-hydroxycholesterol	12-33	sterol O-acyltransferase 1	Homo sapiens	89-93
2590161-4	1989	Addition of 25-hydroxycholesterol to fibroblasts cultured in delipidated serum increased ACAT activity for all three cell types, although stimulation in NPD cells was less than that observed in NPC cells.	25-hydroxycholesterol	12-33	NPC intracellular cholesterol transporter 1	Homo sapiens	194-197
2474437-4	1989	Several findings indicate that estradiol enhances cholesterol transport and availability to the P450scc rather than affects the expression of this enzyme: 1) the difference in mitochondrial progestagen synthesis induced by estradiol was obliterated by the presence of 25-hydroxycholesterol; 2) immunoblotting of P450scc indicated no stimulatory effect of estradiol on the amount of enzyme; and 3) levels of P450scc mRNA were not increased by estradiol.	25-hydroxycholesterol	268-289	cytochrome P450, family 11, subfamily a, polypeptide 1	Rattus norvegicus	96-103
2474437-4	1989	Several findings indicate that estradiol enhances cholesterol transport and availability to the P450scc rather than affects the expression of this enzyme: 1) the difference in mitochondrial progestagen synthesis induced by estradiol was obliterated by the presence of 25-hydroxycholesterol; 2) immunoblotting of P450scc indicated no stimulatory effect of estradiol on the amount of enzyme; and 3) levels of P450scc mRNA were not increased by estradiol.	25-hydroxycholesterol	268-289	cytochrome P450, family 11, subfamily a, polypeptide 1	Rattus norvegicus	312-319
2474437-4	1989	Several findings indicate that estradiol enhances cholesterol transport and availability to the P450scc rather than affects the expression of this enzyme: 1) the difference in mitochondrial progestagen synthesis induced by estradiol was obliterated by the presence of 25-hydroxycholesterol; 2) immunoblotting of P450scc indicated no stimulatory effect of estradiol on the amount of enzyme; and 3) levels of P450scc mRNA were not increased by estradiol.	25-hydroxycholesterol	268-289	cytochrome P450, family 11, subfamily a, polypeptide 1	Rattus norvegicus	312-319
2688859-3	1989	LDL and 25-hydroxycholesterol reduce LDL receptor expression, but this suppressive effect is partially overcome by 8-bromo-cAMP.	25-hydroxycholesterol	8-29	low density lipoprotein receptor	Homo sapiens	37-49
2568358-2	1989	Addition of either delipidized serum and mevinolin or low density lipoprotein, 25-hydroxycholesterol, or mevalonic acid to HepG2 cells resulted in rapid changes both in the levels of the mRNAs and in the rates of synthesis of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase, HMG-CoA reductase, and farnesyl pyrophosphate synthetase (prenyltranferase).	25-hydroxycholesterol	79-100	3-hydroxy-3-methylglutaryl-CoA synthase 2	Homo sapiens	226-282
2568358-2	1989	Addition of either delipidized serum and mevinolin or low density lipoprotein, 25-hydroxycholesterol, or mevalonic acid to HepG2 cells resulted in rapid changes both in the levels of the mRNAs and in the rates of synthesis of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase, HMG-CoA reductase, and farnesyl pyrophosphate synthetase (prenyltranferase).	25-hydroxycholesterol	79-100	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	284-301
2471639-3	1989	The content of LDL-receptor mRNA was reduced when the cells were pre-incubated in medium containing foetal calf serum, 25-hydroxycholesterol, or LDL, compared to that measured in cells which had been pre-incubated in medium containing lipoprotein-deficient serum (LPDS).	25-hydroxycholesterol	119-140	low density lipoprotein receptor	Homo sapiens	15-27
2917146-0	1989	Treatment of CHO-K1 cells with 25-hydroxycholesterol produces a more rapid loss of 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity than can be accounted for by enzyme turnover.	25-hydroxycholesterol	31-52	3-hydroxy-3-methylglutaryl-coenzyme A reductase	Cricetulus griseus	83-130
2598930-4	1989	25-Hydroxycholesterol proved to be a suitable substrate for determining the maximum rate of pregnenolone synthesis by cytochrome P-450scc in isolated mitochondria.	25-hydroxycholesterol	0-21	cytochrome P450 family 11 subfamily A member 1	Homo sapiens	129-137
2777807-1	1989	Feedback repression of the genes encoding the low density lipoprotein receptor and several enzymes of the cholesterol biosynthetic pathway is mediated by 25-hydroxycholesterol and other oxysterols.	25-hydroxycholesterol	154-175	low-density lipoprotein receptor	Oryctolagus cuniculus	46-78
2742872-4	1989	In the presence of nsLTP, the transport of both cholesterol and the oxysterol derivatives was greatly enhanced; the highest rate of transport was observed for 25-hydroxycholesterol.	25-hydroxycholesterol	159-180	sterol carrier protein 2	Homo sapiens	19-24
2742872-5	1989	In the absence of vesicles, binding of cholesterol and of 25-hydroxycholesterol from the monolayer to nsLTP was negligible.	25-hydroxycholesterol	58-79	sterol carrier protein 2	Homo sapiens	102-107
2667453-5	1989	A membrane-bound enzyme, 5"-nucleotidase, was inhibited after 24 to 48 hrs incubation with either 25-OH or triol.	25-hydroxycholesterol	98-103	LOW QUALITY PROTEIN: 5'-nucleotidase	Oryctolagus cuniculus	25-40
2794780-3	1989	In mouse L cell fibroblasts reductase activity was restored with the concomitant metabolism of 25-hydroxycholesterol via side-chain hydroxylation and scission of the C20-C22 bond.	25-hydroxycholesterol	95-116	Sp7 transcription factor 7	Mus musculus	170-173
3241124-1	1988	Treatment of cultured human skin fibroblasts with cycloheximide retarded the down-regulation of low density lipoprotein (LDL) receptor activity caused by 25-hydroxycholesterol.	25-hydroxycholesterol	154-175	low density lipoprotein receptor	Homo sapiens	96-134
2855024-6	1988	Our observations clearly indicate that given the proper hydroxy substrates (22R-hydroxycholesterol or 25-hydroxycholesterol), MA-10 Leydig cells are able to convert them into progesterone without any stimulation by steroidogenic stimuli, i.e. cAMP or hCG.	25-hydroxycholesterol	102-123	hypertrichosis 2 (generalised, congenital)	Homo sapiens	251-254
3390448-2	1988	HMG-CoA reductase mRNA levels increased within 2 h of stimulation and remained elevated for at least 6 h. Treatment of granulosa cells with 25-hydroxycholesterol, a soluble cholesterol analogue, in combination with aminoglutethimide to block conversion of cellular sterols to pregnenolone, resulted in suppression of HMG-CoA reductase mRNA.	25-hydroxycholesterol	140-161	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	0-17
2904178-4	1988	In this report, we demonstrate that this mutant is defective in regulation of the mRNA levels for HMG-CoA reductase and HMG-CoA synthase by 25-hydroxycholesterol and mevinolin.	25-hydroxycholesterol	140-161	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	98-115
3390448-2	1988	HMG-CoA reductase mRNA levels increased within 2 h of stimulation and remained elevated for at least 6 h. Treatment of granulosa cells with 25-hydroxycholesterol, a soluble cholesterol analogue, in combination with aminoglutethimide to block conversion of cellular sterols to pregnenolone, resulted in suppression of HMG-CoA reductase mRNA.	25-hydroxycholesterol	140-161	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	317-334
3390448-3	1988	When cells were stimulated with 8-bromo-cAMP in the presence of 25-hydroxycholesterol and aminoglutethimide, the increase in HMG-CoA reductase mRNA provoked by the tropic agent was markedly attenuated.	25-hydroxycholesterol	64-85	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	125-142
3390448-5	1988	25-Hydroxycholesterol in the presence of aminoglutethimide suppressed low-density lipoprotein (LDL) receptor mRNA, but 8-bromo-cAMP effected a significant stimulation of LDL receptor mRNA levels when added with hydroxysterol and aminoglutethimide.	25-hydroxycholesterol	0-21	low density lipoprotein receptor	Homo sapiens	70-108
6491542-2	1984	Since 25-hydroxycholesterol rapidly stimulated incorporation of [3H]oleate into the cholesteryl ester fraction of these cells, we have tested the possibility that the well-known inhibition of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase) by 25-hydroxycholesterol might be the indirect consequence of an increased cholesterol esterification rather than a direct effect on HMG-CoA reductase.	25-hydroxycholesterol	6-27	3-hydroxy-3-methylglutaryl-Coenzyme A reductase	Mus musculus	192-239
3311719-11	1987	Insulin treatment did not affect P450 SCC activity, whereas IGF-I treatment significantly stimulated P450 SCC activity by 19-36%, as measured by the conversion of 25-hydroxycholesterol to progesterone.	25-hydroxycholesterol	163-184	insulin like growth factor 1	Homo sapiens	60-65
3311719-11	1987	Insulin treatment did not affect P450 SCC activity, whereas IGF-I treatment significantly stimulated P450 SCC activity by 19-36%, as measured by the conversion of 25-hydroxycholesterol to progesterone.	25-hydroxycholesterol	163-184	cytochrome P450 family 11 subfamily A member 1	Homo sapiens	101-109
2445765-0	1987	Differentiation of human keratinocytes: changes in lipid synthesis, plasma membrane lipid composition, and 125I-EGF binding upon administration of 25-hydroxycholesterol and mevinolin.	25-hydroxycholesterol	147-168	epidermal growth factor	Homo sapiens	112-115
2445765-5	1987	The experimental modulation of plasma membrane composition by 25-hydroxycholesterol or mevinolin were accompanied by a decreased cornified envelope formation and by high expression of EGF binding sites.	25-hydroxycholesterol	62-83	epidermal growth factor	Homo sapiens	184-187
3663886-2	1987	In hepatocytes treated with 25-hydroxycholesterol, AcAc-CoA ligase, 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase and rates of sterol synthesis were substantially decreased.	25-hydroxycholesterol	28-49	3-hydroxy-3-methylglutaryl-CoA reductase	Rattus norvegicus	68-119
3320557-4	1987	The tropic hormone effects on LDL receptor mRNA are observed even in the presence of 25-hydroxycholesterol and aminoglutethimide, which by themselves suppress LDL receptor mRNA.	25-hydroxycholesterol	85-106	low density lipoprotein receptor	Homo sapiens	30-42
3320557-4	1987	The tropic hormone effects on LDL receptor mRNA are observed even in the presence of 25-hydroxycholesterol and aminoglutethimide, which by themselves suppress LDL receptor mRNA.	25-hydroxycholesterol	85-106	low density lipoprotein receptor	Homo sapiens	159-171
3760035-2	1986	It was found that short transient exposures to the HMG CoA reductase inhibitor 25-hydroxycholesterol temporarily blocked the cell cycle traverse in the postmitotic half of G1 (G1pm), whereas cells in the subsequent cell cycle phases were unaffected.	25-hydroxycholesterol	79-100	allograft inflammatory factor 1	Mus musculus	172-180
3760035-3	1986	The kinetics of the cell cycle delay, induced by 25-hydroxycholesterol, resembled the kinetics of the delay induced by serum depletion, which also inhibited the activity of HMG CoA reductase.	25-hydroxycholesterol	49-70	3-hydroxy-3-methylglutaryl-Coenzyme A reductase	Mus musculus	173-190
3794551-9	1986	Although 25-hydroxycholesterol induced a decrease in LDL receptor content and synthesis within 6 hr, this action was overridden by simultaneous exposure to hCG.	25-hydroxycholesterol	9-30	low density lipoprotein receptor	Homo sapiens	53-65
3794551-9	1986	Although 25-hydroxycholesterol induced a decrease in LDL receptor content and synthesis within 6 hr, this action was overridden by simultaneous exposure to hCG.	25-hydroxycholesterol	9-30	chorionic gonadotropin subunit beta 5	Homo sapiens	156-159
3707902-5	1986	Cycloheximide under this condition causes an 8-fold increase in ACAT activity; the increase approaches a maximum in 6-8 h. The extent of ACAT activation by cycloheximide inversely depends on exogenous sterol present in the medium; LDL diminishes the activation, while cationized LDL or 25-hydroxycholesterol completely abolishes the activation.	25-hydroxycholesterol	286-307	sterol O-acyltransferase 1	Cricetulus griseus	64-68
3707902-5	1986	Cycloheximide under this condition causes an 8-fold increase in ACAT activity; the increase approaches a maximum in 6-8 h. The extent of ACAT activation by cycloheximide inversely depends on exogenous sterol present in the medium; LDL diminishes the activation, while cationized LDL or 25-hydroxycholesterol completely abolishes the activation.	25-hydroxycholesterol	286-307	sterol O-acyltransferase 1	Cricetulus griseus	137-141
3955082-7	1986	The quantitative (but not qualitative) variations of oxysterol-binding protein could be related to the inhibitory effect of 25-hydroxycholesterol on DNA synthesis, which becomes critical when this sterol is added in the G2M phase of the cell cycle.	25-hydroxycholesterol	124-145	oxysterol binding protein	Rattus norvegicus	53-78
3949765-7	1986	Compound 58-035 also increased the down-regulation of the J774 LDL receptor in the presence of 25-hydroxycholesterol and acetyl-LDL but not in the presence of cholesteryl hemisuccinate, which is not an ACAT substrate.	25-hydroxycholesterol	95-116	low density lipoprotein receptor	Homo sapiens	63-75
3004340-6	1986	25-Hydroxycholesterol, which enters cells without the requirement of low density lipoprotein-receptor binding, inhibited the HMG-CoA reductase activity in familial hypercholesterolemic cells by reversible phosphorylation.	25-hydroxycholesterol	0-21	low density lipoprotein receptor	Homo sapiens	69-101
4055779-6	1985	A combination of 25-hydroxycholesterol and aminoglutethimide resulted in a 60% suppression of label incorporation into mature LDL receptor compared to untreated cells.	25-hydroxycholesterol	17-38	low density lipoprotein receptor	Homo sapiens	126-138
2449243-0	1988	25-Hydroxycholesterol promotes myelin basic protein-induced leakage of phospholipid vesicles.	25-hydroxycholesterol	0-21	myelin basic protein	Homo sapiens	31-51
2840565-2	1987	After 1 h of treatment with 8-bromo-cAMP, an appreciable increase in hybridizable LDL receptor mRNA was found which increased to apparently maximal levels within 4-6 h. Treatment of the granulosa cells with 25-hydroxycholesterol, in the presence of aminoglutethimide, resulted in a reduction in LDL receptor mRNA content within 6 h of treatment.	25-hydroxycholesterol	207-228	low density lipoprotein receptor	Homo sapiens	82-94
2840565-2	1987	After 1 h of treatment with 8-bromo-cAMP, an appreciable increase in hybridizable LDL receptor mRNA was found which increased to apparently maximal levels within 4-6 h. Treatment of the granulosa cells with 25-hydroxycholesterol, in the presence of aminoglutethimide, resulted in a reduction in LDL receptor mRNA content within 6 h of treatment.	25-hydroxycholesterol	207-228	low density lipoprotein receptor	Homo sapiens	295-307
3760035-2	1986	It was found that short transient exposures to the HMG CoA reductase inhibitor 25-hydroxycholesterol temporarily blocked the cell cycle traverse in the postmitotic half of G1 (G1pm), whereas cells in the subsequent cell cycle phases were unaffected.	25-hydroxycholesterol	79-100	3-hydroxy-3-methylglutaryl-Coenzyme A reductase	Mus musculus	51-68
2991417-5	1985	The glucocorticoid-induced enhancement of LDL receptor and cholesterol synthesis is abolished by preincubation of the cells with dexamethasone in combination with 25-hydroxycholesterol.	25-hydroxycholesterol	163-184	low density lipoprotein receptor	Homo sapiens	42-54
3967750-3	1985	In a previous study the authors demonstrated monotypic foci, all of timidus type, in the atherosclerotic lesions and normal aortic tissue of glucose-6-phosphate dehydrogenase (G-6-PD) mosaic hares fed 25-hydroxycholesterol or triol in addition to a high-cholesterol diet.	25-hydroxycholesterol	201-222	glucose-6-phosphate dehydrogenase	Homo sapiens	141-174
3967750-3	1985	In a previous study the authors demonstrated monotypic foci, all of timidus type, in the atherosclerotic lesions and normal aortic tissue of glucose-6-phosphate dehydrogenase (G-6-PD) mosaic hares fed 25-hydroxycholesterol or triol in addition to a high-cholesterol diet.	25-hydroxycholesterol	201-222	glucose-6-phosphate dehydrogenase	Homo sapiens	176-182
6491542-3	1984	The experimental results show that progesterone, an inhibitor of acyl-CoA:cholesterol acyltransferase (ACAT), when added together with 25-hydroxycholesterol, abolished the increased cholesterol esterification without affecting the inhibition of HMG-CoA reductase by 25-hydroxycholesterol.	25-hydroxycholesterol	135-156	sterol O-acyltransferase 2	Mus musculus	103-107
6491542-3	1984	The experimental results show that progesterone, an inhibitor of acyl-CoA:cholesterol acyltransferase (ACAT), when added together with 25-hydroxycholesterol, abolished the increased cholesterol esterification without affecting the inhibition of HMG-CoA reductase by 25-hydroxycholesterol.	25-hydroxycholesterol	266-287	sterol O-acyltransferase 2	Mus musculus	65-101
6491542-3	1984	The experimental results show that progesterone, an inhibitor of acyl-CoA:cholesterol acyltransferase (ACAT), when added together with 25-hydroxycholesterol, abolished the increased cholesterol esterification without affecting the inhibition of HMG-CoA reductase by 25-hydroxycholesterol.	25-hydroxycholesterol	266-287	sterol O-acyltransferase 2	Mus musculus	103-107
6195162-2	1983	25-Hydroxycholesterol inhibits cholesterol biosynthesis by inhibiting the activity of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase.	25-hydroxycholesterol	0-21	3-hydroxy-3-methylglutaryl-CoA reductase	Gallus gallus	86-143
6177525-10	1982	In the presence of 5-cholesten-3 beta, 25-diol, the magnitude of the synergism between E2 and FSH was also increased markedly.	25-hydroxycholesterol	19-46	FSH	Sus scrofa	87-97
6193139-9	1983	Insulin also enhanced progesterone production in the presence of a soluble sterol substrate, 5-cholesten-3beta,25-diol, which readily gains access to the mitochondrial cholesterol side-chain cleavage system.	25-hydroxycholesterol	93-118	insulin	Sus scrofa	0-7
6631233-0	1983	Regulation of acyl CoA:cholesterol acyltransferase by 25-hydroxycholesterol in rabbit intestinal microsomes and absorptive cells.	25-hydroxycholesterol	54-75	sterol O-acyltransferase 1	Oryctolagus cuniculus	14-50
6631233-1	1983	The regulation of rabbit intestinal acyl CoA:cholesterol acyltransferase (ACAT) by 25-hydroxycholesterol was studied.	25-hydroxycholesterol	83-104	sterol O-acyltransferase 1	Oryctolagus cuniculus	36-72
6631233-1	1983	The regulation of rabbit intestinal acyl CoA:cholesterol acyltransferase (ACAT) by 25-hydroxycholesterol was studied.	25-hydroxycholesterol	83-104	sterol O-acyltransferase 1	Oryctolagus cuniculus	74-78
6631233-2	1983	25-Hydroxycholesterol significantly increased jejunal microsomal ACAT activity.	25-hydroxycholesterol	0-21	sterol O-acyltransferase 1	Oryctolagus cuniculus	65-69
6631233-3	1983	The stimulation of ACAT activity by 25-hydroxycholesterol was inversely related to microsomal cholesterol content.	25-hydroxycholesterol	36-57	sterol O-acyltransferase 1	Oryctolagus cuniculus	19-23
6631233-11	1983	We conclude that 25-hydroxycholesterol increases intestinal ACAT activity.	25-hydroxycholesterol	17-38	sterol O-acyltransferase 1	Oryctolagus cuniculus	60-64
6631233-12	1983	The effect of 25-hydroxycholesterol on ACAT is dependent upon the availability of cholesterol to the enzyme.	25-hydroxycholesterol	14-35	sterol O-acyltransferase 1	Oryctolagus cuniculus	39-43
7126620-1	1982	The activity of the rate-limiting enzyme of the cholesterol biosynthetic pathway, 3-hydroxy-3-methylglutaryl coenzyme A reductase in Chinese hamster ovary (CHO) cells decreased more rapidly in cells treated with 25-hydroxycholesterol alone (t 1/2 = 1.5 h) than in those incubated with cycloheximide alone (t 1/2 = 5 h).	25-hydroxycholesterol	212-233	3-hydroxy-3-methylglutaryl-coenzyme A reductase	Cricetulus griseus	82-129
6177525-13	1982	Studies with 5-cholesten-3 beta, 25-diol suggest that estrogen and FSH exert significant stimulatory effects at the level of cholesterol side-chain cleavage.	25-hydroxycholesterol	13-40	FSH	Sus scrofa	67-70
7417482-0	1980	Studies on the mechanisms of the rapid modulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase in intact liver by mevalonolactone and 25-hydroxycholesterol.	25-hydroxycholesterol	140-161	3-hydroxy-3-methylglutaryl-CoA reductase	Rattus norvegicus	53-100
7260908-1	1981	The effects of 25-hydroxycholesterol (25-OHC), a potent inhibitor of sterol synthesis, on the growth, viability, and sterol content of C-6 rat glioma cells have been studied.	25-hydroxycholesterol	15-36	complement C6	Rattus norvegicus	135-138
7260908-1	1981	The effects of 25-hydroxycholesterol (25-OHC), a potent inhibitor of sterol synthesis, on the growth, viability, and sterol content of C-6 rat glioma cells have been studied.	25-hydroxycholesterol	38-44	complement C6	Rattus norvegicus	135-138
7260908-4	1981	In contrast, C-6 cells that were induced to enter a quiescent state by removal of serum from the medium remained viable and morphologically differentiated in the presence of 25-OHC.	25-hydroxycholesterol	174-180	complement C6	Rattus norvegicus	13-16
7260908-6	1981	the selective killing of proliferating C-6 glioma cells by 25-OHC was correlated with a 45 to 50% decline in the sterol/phospholipid molar ratio, whereas the sterol/phospholipid ratio in the quiescent cells was not affected by 25-OHC.	25-hydroxycholesterol	59-65	complement C6	Rattus norvegicus	39-42
7260908-6	1981	the selective killing of proliferating C-6 glioma cells by 25-OHC was correlated with a 45 to 50% decline in the sterol/phospholipid molar ratio, whereas the sterol/phospholipid ratio in the quiescent cells was not affected by 25-OHC.	25-hydroxycholesterol	227-233	complement C6	Rattus norvegicus	39-42
7202688-2	1981	Changes in the ratios of G-6-PD types in skin fibroblast cultures exposed to 25-hydroxycholesterol.	25-hydroxycholesterol	77-98	glucose-6-phosphate dehydrogenase	Homo sapiens	25-31
7240196-0	1981	Evidence indicating that inactivation of 3-hydroxy-3-methylglutaryl coenzyme A reductase by low density lipoprotein or by 25-hydroxycholesterol requires mediator protein(s) with rapid turnover rate.	25-hydroxycholesterol	122-143	3-hydroxy-3-methylglutaryl-coenzyme A reductase	Cricetulus griseus	41-88
7417482-10	1980	Perfusion of 25-hydroxycholesterol through livers isolated from animals at the circadian peak of cholesterol biosynthesis resulted in a rapid, 75-80% inhibition of 3-hydroxy-3-methylglutaryl coenzyme A reductase.	25-hydroxycholesterol	13-34	3-hydroxy-3-methylglutaryl-CoA reductase	Rattus norvegicus	164-211
489551-2	1979	Primary rat hepatocyte culture cells were used to study the acute regulation of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activity in response to 25-hydroxycholesterol, 3 beta,5 alpha,6 beta-cholestantriol, and mevalonolactone.	25-hydroxycholesterol	162-183	3-hydroxy-3-methylglutaryl-CoA reductase	Rattus norvegicus	80-137
7356845-3	1980	In normal fibroblasts preincubated in lipoprotein-deficient serum, LDL or 25-hydroxycholesterol decreased cholesterol synthesis and LDL receptor activity and increased cholesterol ester formation.	25-hydroxycholesterol	74-95	low density lipoprotein receptor	Homo sapiens	132-144
228272-5	1979	The addition of a mixture of 25-hydroxycholesterol and cholesterol suppressed squalene synthetase equally well in normal and mutant fibroblasts.	25-hydroxycholesterol	29-50	farnesyl-diphosphate farnesyltransferase 1	Homo sapiens	78-97
33845332-5	2021	Further, the anti-SVA effect of 25-hydroxycholesterol (25HC), which is the product of CH25H, operates via inhibition of viral attachment and replication.	25-hydroxycholesterol	32-53	cholesterol 25-hydroxylase	Homo sapiens	86-91
167000-6	1975	Two distinct steroid binding sites on cytochrome P-450scc were detected by, respectively, the slow type I response to cholest-5-ene-3beta, 25-diol (25-hydroxycholesterol) and the rapid type I response to a subsequent addition of cholest-5-ene-3beta, 20alpha, 22 R-triol (20alpha, 22R-dihydroxycholesterol).	25-hydroxycholesterol	118-146	cytochrome P450, family 11, subfamily a, polypeptide 1	Rattus norvegicus	49-57
167000-6	1975	Two distinct steroid binding sites on cytochrome P-450scc were detected by, respectively, the slow type I response to cholest-5-ene-3beta, 25-diol (25-hydroxycholesterol) and the rapid type I response to a subsequent addition of cholest-5-ene-3beta, 20alpha, 22 R-triol (20alpha, 22R-dihydroxycholesterol).	25-hydroxycholesterol	148-169	cytochrome P450, family 11, subfamily a, polypeptide 1	Rattus norvegicus	49-57
34057681-1	2021	Cholesterol-25-hydroxylase (CH25H) and its enzymatic product 25-hydroxycholesterol (25HC) exert broadly antiviral activity including inhibiting HIV-1 infection.	25-hydroxycholesterol	61-82	cholesterol 25-hydroxylase	Homo sapiens	0-26
34057681-1	2021	Cholesterol-25-hydroxylase (CH25H) and its enzymatic product 25-hydroxycholesterol (25HC) exert broadly antiviral activity including inhibiting HIV-1 infection.	25-hydroxycholesterol	61-82	cholesterol 25-hydroxylase	Homo sapiens	28-33
34057681-1	2021	Cholesterol-25-hydroxylase (CH25H) and its enzymatic product 25-hydroxycholesterol (25HC) exert broadly antiviral activity including inhibiting HIV-1 infection.	25-hydroxycholesterol	84-88	cholesterol 25-hydroxylase	Homo sapiens	0-26
34057681-1	2021	Cholesterol-25-hydroxylase (CH25H) and its enzymatic product 25-hydroxycholesterol (25HC) exert broadly antiviral activity including inhibiting HIV-1 infection.	25-hydroxycholesterol	84-88	cholesterol 25-hydroxylase	Homo sapiens	28-33
33845332-5	2021	Further, the anti-SVA effect of 25-hydroxycholesterol (25HC), which is the product of CH25H, operates via inhibition of viral attachment and replication.	25-hydroxycholesterol	55-59	cholesterol 25-hydroxylase	Homo sapiens	86-91
33878616-7	2021	Mechanistically, 25HC pretreatment activated PINK1/Parkin dependent mitophagy and inhibited the NLRP3 inflammasome.	25-hydroxycholesterol	17-21	PTEN induced kinase 1	Rattus norvegicus	45-50
33878616-7	2021	Mechanistically, 25HC pretreatment activated PINK1/Parkin dependent mitophagy and inhibited the NLRP3 inflammasome.	25-hydroxycholesterol	17-21	NLR family, pyrin domain containing 3	Rattus norvegicus	96-101
33878616-9	2021	In conclusion, 25HC pretreatment ameliorates rat hepatic I/R injury, and this protective effect may be dependent on activating mitophagy and inhibiting NLRP3 inflammasome activation.	25-hydroxycholesterol	15-19	NLR family, pyrin domain containing 3	Rattus norvegicus	152-157
32913007-9	2020	Acute intrathecal injection of the GPR183 ligand 7alpha,25-OHC in naive mice induced dose-dependent allodynia.	25-hydroxycholesterol	56-62	G protein-coupled receptor 183	Mus musculus	35-41
33476696-5	2021	Treatment with 25-hydroxycholesterol (25HC), the catalysate of cholesterol via CH25H, inhibited PDCoV proliferation by impairing viral invasion of IPI-FX cells.	25-hydroxycholesterol	15-36	cholesterol 25-hydroxylase	Homo sapiens	79-84
33476696-5	2021	Treatment with 25-hydroxycholesterol (25HC), the catalysate of cholesterol via CH25H, inhibited PDCoV proliferation by impairing viral invasion of IPI-FX cells.	25-hydroxycholesterol	38-42	cholesterol 25-hydroxylase	Homo sapiens	79-84
33446483-3	2021	25-hydroxycholesterol (25HC)-dependent association of Scap and Insigs acts as the master switch for the SREBP pathway.	25-hydroxycholesterol	0-21	SREBF chaperone	Homo sapiens	54-58
33446483-3	2021	25-hydroxycholesterol (25HC)-dependent association of Scap and Insigs acts as the master switch for the SREBP pathway.	25-hydroxycholesterol	23-27	SREBF chaperone	Homo sapiens	54-58
33446483-6	2021	A 25HC molecule is sandwiched between the S4-S6 segments in Scap and TMs 3/4 in Insig-2 in the luminal leaflet of the membrane.	25-hydroxycholesterol	2-6	SREBF chaperone	Homo sapiens	60-64
33446483-6	2021	A 25HC molecule is sandwiched between the S4-S6 segments in Scap and TMs 3/4 in Insig-2 in the luminal leaflet of the membrane.	25-hydroxycholesterol	2-6	tropomyosin 3	Homo sapiens	69-76
33446483-6	2021	A 25HC molecule is sandwiched between the S4-S6 segments in Scap and TMs 3/4 in Insig-2 in the luminal leaflet of the membrane.	25-hydroxycholesterol	2-6	insulin induced gene 2	Homo sapiens	80-87
33717065-2	2021	In mammalian cells virus infections lead to the accumulation of the oxysterol 25-hydroxycholesterol (25HC), an antiviral factor, which is produced from cholesterol by the cholesterol 25 hydroxylase (CH25H).	25-hydroxycholesterol	101-105	cholesterol 25-hydroxylase	Homo sapiens	171-197
33717065-2	2021	In mammalian cells virus infections lead to the accumulation of the oxysterol 25-hydroxycholesterol (25HC), an antiviral factor, which is produced from cholesterol by the cholesterol 25 hydroxylase (CH25H).	25-hydroxycholesterol	101-105	cholesterol 25-hydroxylase	Homo sapiens	199-204
33239446-2	2020	Here, using an IFN-stimulated gene screen against vesicular stomatitis virus (VSV)-SARS-CoV and VSV-SARS-CoV-2 chimeric viruses, we identified CH25H and its enzymatic product 25-hydroxycholesterol (25HC) as potent inhibitors of SARS-CoV-2 replication.	25-hydroxycholesterol	175-196	cholesterol 25-hydroxylase	Homo sapiens	143-148
33239446-2	2020	Here, using an IFN-stimulated gene screen against vesicular stomatitis virus (VSV)-SARS-CoV and VSV-SARS-CoV-2 chimeric viruses, we identified CH25H and its enzymatic product 25-hydroxycholesterol (25HC) as potent inhibitors of SARS-CoV-2 replication.	25-hydroxycholesterol	198-202	cholesterol 25-hydroxylase	Homo sapiens	143-148
33239446-3	2020	Internalized 25HC accumulates in the late endosomes and potentially restricts SARS-CoV-2 spike protein catalyzed membrane fusion via blockade of cholesterol export.	25-hydroxycholesterol	13-17	surface glycoprotein	Severe acute respiratory syndrome coronavirus 2	89-94
32921410-0	2020	Corrigendum to "25-hydroxycholesterol promotes RANKL-induced osteoclastogenesis through coordinating NFATc1 and Sp1 complex in the transcription of miR-139-5p .	25-hydroxycholesterol	16-37	TNF superfamily member 11	Homo sapiens	47-52
32921410-0	2020	Corrigendum to "25-hydroxycholesterol promotes RANKL-induced osteoclastogenesis through coordinating NFATc1 and Sp1 complex in the transcription of miR-139-5p .	25-hydroxycholesterol	16-37	nuclear factor of activated T cells 1	Homo sapiens	101-107
32229247-4	2020	Cholesterol 25-Hydroxylase (CH25H) converts cholesterol into 25-Hydroxycholesterol (25-HC), an oxysterol that modulates immune responses.	25-hydroxycholesterol	61-82	cholesterol 25-hydroxylase	Homo sapiens	0-26
32777483-14	2020	Finally, exogenous 25-hydroxycholesterol esterification within alveolar macrophages prepared from wild-type mice was significantly higher than that from LPLA2 deficient mice.	25-hydroxycholesterol	19-40	phospholipase A2, group XV	Mus musculus	153-158
32501835-1	2020	BACKGROUND: 25-hydroxylase (CH25H) is an Interferon stimulated gene (ISG), which catalyzes the synthesis of 25-Hydroxycholesterol (25HC).	25-hydroxycholesterol	108-129	cholesterol 25-hydroxylase	Homo sapiens	28-33
32501835-1	2020	BACKGROUND: 25-hydroxylase (CH25H) is an Interferon stimulated gene (ISG), which catalyzes the synthesis of 25-Hydroxycholesterol (25HC).	25-hydroxycholesterol	131-135	cholesterol 25-hydroxylase	Homo sapiens	28-33
32229247-4	2020	Cholesterol 25-Hydroxylase (CH25H) converts cholesterol into 25-Hydroxycholesterol (25-HC), an oxysterol that modulates immune responses.	25-hydroxycholesterol	61-82	cholesterol 25-hydroxylase	Homo sapiens	28-33
32423869-8	2020	Moreover, knockdown of CHOP by siRNA blocked 25-HC-induced mineral deposition in VSMCs.	25-hydroxycholesterol	45-50	DNA-damage inducible transcript 3	Rattus norvegicus	23-27
32859970-1	2020	Cholesterol 25-hydroxylase (CH25H) encodes the enzyme that converts cholesterol to 25-hydroxycholesterol (25-HC).	25-hydroxycholesterol	83-104	cholesterol 25-hydroxylase	Mus musculus	0-26
32859970-1	2020	Cholesterol 25-hydroxylase (CH25H) encodes the enzyme that converts cholesterol to 25-hydroxycholesterol (25-HC).	25-hydroxycholesterol	83-104	cholesterol 25-hydroxylase	Mus musculus	28-33
32859970-1	2020	Cholesterol 25-hydroxylase (CH25H) encodes the enzyme that converts cholesterol to 25-hydroxycholesterol (25-HC).	25-hydroxycholesterol	106-111	cholesterol 25-hydroxylase	Mus musculus	0-26
32859970-1	2020	Cholesterol 25-hydroxylase (CH25H) encodes the enzyme that converts cholesterol to 25-hydroxycholesterol (25-HC).	25-hydroxycholesterol	106-111	cholesterol 25-hydroxylase	Mus musculus	28-33
32859970-5	2020	The function of CH25H was investigated using loss-of-function and gain-of-function such as Ch25h-deficient mice, supplementation with exogenous 25-HC and treatment of 25-HC into Caco2 and HCT116 colonic epithelial cells.	25-hydroxycholesterol	144-149	cholesterol 25-hydroxylase	Mus musculus	16-21
32423869-9	2020	Collectively, this study for the first time demonstrates that 25-HC promotes vascular calcification via ATF4/CHOP signaling using in vitro and ex vivo models, suggesting that ERS is involved in the regulation of 25-HC-induced vascular calcification.	25-hydroxycholesterol	62-67	activating transcription factor 4	Rattus norvegicus	104-108
32423869-9	2020	Collectively, this study for the first time demonstrates that 25-HC promotes vascular calcification via ATF4/CHOP signaling using in vitro and ex vivo models, suggesting that ERS is involved in the regulation of 25-HC-induced vascular calcification.	25-hydroxycholesterol	62-67	DNA-damage inducible transcript 3	Rattus norvegicus	109-113
32343675-2	2020	AM expression of cholesterol-25-hydroxylase (CH25H), the primary biosynthetic enzyme for 25-hydroxycholesterol (25HC), far exceeds that of macrophages in other tissues, but no role for CH25H has been defined in lung biology.	25-hydroxycholesterol	89-110	cholesterol 25-hydroxylase	Mus musculus	17-43
32640529-2	2020	Cholesterol-25-hydroxylase (CH25H) and its enzymatic products 25-hydroxycholesterol (25HC), a well-known oxysterol that regulates lipid metabolism, have been reported to play multiple functions in modulating cholesterol homeostasis, inflammation, and immune responses.	25-hydroxycholesterol	62-83	cholesterol 25-hydroxylase	Homo sapiens	0-26
32640529-2	2020	Cholesterol-25-hydroxylase (CH25H) and its enzymatic products 25-hydroxycholesterol (25HC), a well-known oxysterol that regulates lipid metabolism, have been reported to play multiple functions in modulating cholesterol homeostasis, inflammation, and immune responses.	25-hydroxycholesterol	62-83	cholesterol 25-hydroxylase	Homo sapiens	28-33
32640529-2	2020	Cholesterol-25-hydroxylase (CH25H) and its enzymatic products 25-hydroxycholesterol (25HC), a well-known oxysterol that regulates lipid metabolism, have been reported to play multiple functions in modulating cholesterol homeostasis, inflammation, and immune responses.	25-hydroxycholesterol	85-89	cholesterol 25-hydroxylase	Homo sapiens	0-26
32640529-2	2020	Cholesterol-25-hydroxylase (CH25H) and its enzymatic products 25-hydroxycholesterol (25HC), a well-known oxysterol that regulates lipid metabolism, have been reported to play multiple functions in modulating cholesterol homeostasis, inflammation, and immune responses.	25-hydroxycholesterol	85-89	cholesterol 25-hydroxylase	Homo sapiens	28-33
32552741-0	2020	25-Hydroxycholesterol amplifies microglial IL-1beta production in an apoE isoform-dependent manner.	25-hydroxycholesterol	0-21	interleukin 1 alpha	Mus musculus	43-51
32552741-0	2020	25-Hydroxycholesterol amplifies microglial IL-1beta production in an apoE isoform-dependent manner.	25-hydroxycholesterol	0-21	apolipoprotein E	Mus musculus	69-73
32552741-4	2020	Cholesterol 25-hydroxylase (CH25H), the enzyme responsible for 25-HC production, has also been found to be one of the disease-associated microglial (DAM) genes that are upregulated in the brain of AD and AD transgenic mouse models.	25-hydroxycholesterol	63-68	cholesterol 25-hydroxylase	Mus musculus	0-26
32552741-4	2020	Cholesterol 25-hydroxylase (CH25H), the enzyme responsible for 25-HC production, has also been found to be one of the disease-associated microglial (DAM) genes that are upregulated in the brain of AD and AD transgenic mouse models.	25-hydroxycholesterol	63-68	cholesterol 25-hydroxylase	Mus musculus	28-33
32395076-1	2020	The aim of the present study was to investigate the pathophysiological etiology of osteoarthritis that is mediated by the apoptosis of chondrocytes exposed to 25-hydroxycholesterol (25-HC), an oxysterol synthesized by the expression of cholesterol-25-hydroxylase (CH25H) under inflammatory conditions.	25-hydroxycholesterol	159-180	cholesterol 25-hydroxylase	Rattus norvegicus	236-262
32343675-2	2020	AM expression of cholesterol-25-hydroxylase (CH25H), the primary biosynthetic enzyme for 25-hydroxycholesterol (25HC), far exceeds that of macrophages in other tissues, but no role for CH25H has been defined in lung biology.	25-hydroxycholesterol	89-110	cholesterol 25-hydroxylase	Mus musculus	45-50
32343675-3	2020	As 25HC is an agonist for the anti-inflammatory nuclear receptor, Liver X Receptor (LXR), we speculated that CH25H might regulate inflammatory homeostasis in the lung.	25-hydroxycholesterol	3-7	nuclear receptor subfamily 1, group H, member 3	Mus musculus	66-82
32343675-3	2020	As 25HC is an agonist for the anti-inflammatory nuclear receptor, Liver X Receptor (LXR), we speculated that CH25H might regulate inflammatory homeostasis in the lung.	25-hydroxycholesterol	3-7	nuclear receptor subfamily 1, group H, member 3	Mus musculus	84-87
32343675-3	2020	As 25HC is an agonist for the anti-inflammatory nuclear receptor, Liver X Receptor (LXR), we speculated that CH25H might regulate inflammatory homeostasis in the lung.	25-hydroxycholesterol	3-7	cholesterol 25-hydroxylase	Mus musculus	109-114
32343675-5	2020	Ch25h-/- mice fail to induce 25HC and LXR target genes in the lung after LPS inhalation and exhibit delayed resolution of airway neutrophilia which can be rescued by systemic treatment with either 25HC or synthetic LXR agonists.	25-hydroxycholesterol	29-33	cholesterol 25-hydroxylase	Mus musculus	0-5
32408171-2	2020	The results indicate that HG leads to an increase in nuclear 25-hydroxycholesterol (25HC), which specifically activates DNA methyltransferase-1 (DNMT1), and regulates gene expression involved in intracellular lipid metabolism.	25-hydroxycholesterol	61-82	DNA methyltransferase 1	Homo sapiens	120-143
32408171-2	2020	The results indicate that HG leads to an increase in nuclear 25-hydroxycholesterol (25HC), which specifically activates DNA methyltransferase-1 (DNMT1), and regulates gene expression involved in intracellular lipid metabolism.	25-hydroxycholesterol	61-82	DNA methyltransferase 1	Homo sapiens	145-150
32408171-2	2020	The results indicate that HG leads to an increase in nuclear 25-hydroxycholesterol (25HC), which specifically activates DNA methyltransferase-1 (DNMT1), and regulates gene expression involved in intracellular lipid metabolism.	25-hydroxycholesterol	84-88	DNA methyltransferase 1	Homo sapiens	120-143
32408171-2	2020	The results indicate that HG leads to an increase in nuclear 25-hydroxycholesterol (25HC), which specifically activates DNA methyltransferase-1 (DNMT1), and regulates gene expression involved in intracellular lipid metabolism.	25-hydroxycholesterol	84-88	DNA methyltransferase 1	Homo sapiens	145-150
32392916-1	2020	The aim of the present study was to investigate the pathophysiological etiology of osteoarthritis that is mediated by the apoptosis of chondrocytes exposed to 25-hydroxycholesterol (25-HC), an oxysterol synthesized by the expression of cholesterol-25-hydroxylase (CH25H) under inflammatory conditions.	25-hydroxycholesterol	159-180	cholesterol 25-hydroxylase	Rattus norvegicus	236-262
32392916-1	2020	The aim of the present study was to investigate the pathophysiological etiology of osteoarthritis that is mediated by the apoptosis of chondrocytes exposed to 25-hydroxycholesterol (25-HC), an oxysterol synthesized by the expression of cholesterol-25-hydroxylase (CH25H) under inflammatory conditions.	25-hydroxycholesterol	159-180	cholesterol 25-hydroxylase	Rattus norvegicus	264-269
32392916-1	2020	The aim of the present study was to investigate the pathophysiological etiology of osteoarthritis that is mediated by the apoptosis of chondrocytes exposed to 25-hydroxycholesterol (25-HC), an oxysterol synthesized by the expression of cholesterol-25-hydroxylase (CH25H) under inflammatory conditions.	25-hydroxycholesterol	182-187	cholesterol 25-hydroxylase	Rattus norvegicus	236-262
32392916-1	2020	The aim of the present study was to investigate the pathophysiological etiology of osteoarthritis that is mediated by the apoptosis of chondrocytes exposed to 25-hydroxycholesterol (25-HC), an oxysterol synthesized by the expression of cholesterol-25-hydroxylase (CH25H) under inflammatory conditions.	25-hydroxycholesterol	182-187	cholesterol 25-hydroxylase	Rattus norvegicus	264-269
32392916-3	2020	Furthermore, the production of 25-HC increased in the chondrocytes treated with interleukin-1beta through the expression of CH25H.	25-hydroxycholesterol	31-36	interleukin 1 beta	Rattus norvegicus	80-97
32392916-3	2020	Furthermore, the production of 25-HC increased in the chondrocytes treated with interleukin-1beta through the expression of CH25H.	25-hydroxycholesterol	31-36	cholesterol 25-hydroxylase	Rattus norvegicus	124-129
32392916-6	2020	Moreover, the activity and expression of caspase-3 were increased by the death ligand-mediated extrinsic and mitochondria-dependent intrinsic apoptotic pathways in the chondrocytes treated with 25-HC.	25-hydroxycholesterol	194-199	caspase 3	Rattus norvegicus	41-50
32395076-1	2020	The aim of the present study was to investigate the pathophysiological etiology of osteoarthritis that is mediated by the apoptosis of chondrocytes exposed to 25-hydroxycholesterol (25-HC), an oxysterol synthesized by the expression of cholesterol-25-hydroxylase (CH25H) under inflammatory conditions.	25-hydroxycholesterol	159-180	cholesterol 25-hydroxylase	Rattus norvegicus	264-269
32395076-1	2020	The aim of the present study was to investigate the pathophysiological etiology of osteoarthritis that is mediated by the apoptosis of chondrocytes exposed to 25-hydroxycholesterol (25-HC), an oxysterol synthesized by the expression of cholesterol-25-hydroxylase (CH25H) under inflammatory conditions.	25-hydroxycholesterol	182-187	cholesterol 25-hydroxylase	Rattus norvegicus	236-262
32395076-1	2020	The aim of the present study was to investigate the pathophysiological etiology of osteoarthritis that is mediated by the apoptosis of chondrocytes exposed to 25-hydroxycholesterol (25-HC), an oxysterol synthesized by the expression of cholesterol-25-hydroxylase (CH25H) under inflammatory conditions.	25-hydroxycholesterol	182-187	cholesterol 25-hydroxylase	Rattus norvegicus	264-269
32395076-3	2020	Furthermore, the production of 25-HC increased in the chondrocytes treated with interleukin-1beta through the expression of CH25H.	25-hydroxycholesterol	31-36	interleukin 1 beta	Rattus norvegicus	80-97
32395076-3	2020	Furthermore, the production of 25-HC increased in the chondrocytes treated with interleukin-1beta through the expression of CH25H.	25-hydroxycholesterol	31-36	cholesterol 25-hydroxylase	Rattus norvegicus	124-129
32395076-6	2020	Moreover, the activity and expression of caspase-3 were increased by the death ligand-mediated extrinsic and mitochondria-dependent intrinsic apoptotic pathways in the chondrocytes treated with 25-HC.	25-hydroxycholesterol	194-199	caspase 3	Rattus norvegicus	41-50
31929757-0	2020	25-Hydroxycholesterol protects against myocardial ischemia-reperfusion injury via inhibiting PARP activity.	25-hydroxycholesterol	0-21	poly (ADP-ribose) polymerase family, member 1	Mus musculus	93-97
32258442-0	2020	Spermine synthesis inhibitor blocks 25-hydroxycholesterol-induced- apoptosis via SREBP2 upregulation in DLD-1 cell spheroids.	25-hydroxycholesterol	36-57	sterol regulatory element binding transcription factor 2	Homo sapiens	81-87
32292343-2	2020	PXR may also regulate 25-hydroxycholesterol and 27-hydroxycholesterol.	25-hydroxycholesterol	22-43	nuclear receptor subfamily 1 group I member 2	Homo sapiens	0-3
31902650-3	2020	While 25-hydroxycholesterol induces both HMGCR degradation and SREBP inhibition in a nonselective manner, lanosterol selectively induces HMGCR degradation.	25-hydroxycholesterol	6-27	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	41-46
31801667-8	2020	Lastly, we proved that Ch25h/25 hydroxycholesterol (25 HC)/Liver X receptor alpha (LXRalpha) suppressed EndoMT.	25-hydroxycholesterol	29-50	nuclear receptor subfamily 1 group H member 3	Homo sapiens	83-91
32014920-5	2020	MATERIALS AND METHODS: 25-HC-induced apoptosis was investigated by cell cytotoxicity assay using MTT, cell viability assay using cell LIVE/DEAD cell viability assay, haematoxylin &amp; eosin staining, nuclear staining, fluorescence-activated cell sorting, western blotting using specific antibodies associated with extrinsic and intrinsic apoptosis pathways, and caspase-3/-7 activity assay in FaDu cells.	25-hydroxycholesterol	23-28	caspase 3	Homo sapiens	363-372
31521631-0	2020	Binding and intracellular transport of 25-hydroxycholesterol by Niemann-Pick C2 protein.	25-hydroxycholesterol	39-60	NPC intracellular cholesterol transporter 2	Homo sapiens	64-87
31929757-6	2020	We found that 25-HC significantly reduced the IR-induced infarct size and improved cardiac function, and this protective effect was associated with reduced phosphorylation of p38-MAPK and JNK1/2.	25-hydroxycholesterol	14-19	mitogen-activated protein kinase 14	Mus musculus	175-178
31929757-6	2020	We found that 25-HC significantly reduced the IR-induced infarct size and improved cardiac function, and this protective effect was associated with reduced phosphorylation of p38-MAPK and JNK1/2.	25-hydroxycholesterol	14-19	mitogen-activated protein kinase 8	Mus musculus	188-194
31929757-7	2020	Besides, 25-HC also inhibited the Bax/Bcl-2 ratio and the relative expression of cleaved caspase-3.	25-hydroxycholesterol	9-14	BCL2-associated X protein	Mus musculus	34-37
31929757-7	2020	Besides, 25-HC also inhibited the Bax/Bcl-2 ratio and the relative expression of cleaved caspase-3.	25-hydroxycholesterol	9-14	B cell leukemia/lymphoma 2	Mus musculus	38-43
31929757-8	2020	Furthermore, 25-HC decreased the PARP activity, indicating that 25-HC ameliorates IR injury via the PARP pathway.	25-hydroxycholesterol	13-18	poly (ADP-ribose) polymerase family, member 1	Mus musculus	33-37
31929757-8	2020	Furthermore, 25-HC decreased the PARP activity, indicating that 25-HC ameliorates IR injury via the PARP pathway.	25-hydroxycholesterol	13-18	poly (ADP-ribose) polymerase family, member 1	Mus musculus	100-104
31929757-8	2020	Furthermore, 25-HC decreased the PARP activity, indicating that 25-HC ameliorates IR injury via the PARP pathway.	25-hydroxycholesterol	64-69	poly (ADP-ribose) polymerase family, member 1	Mus musculus	33-37
31929757-8	2020	Furthermore, 25-HC decreased the PARP activity, indicating that 25-HC ameliorates IR injury via the PARP pathway.	25-hydroxycholesterol	64-69	poly (ADP-ribose) polymerase family, member 1	Mus musculus	100-104
31929757-9	2020	The 25-HC group abolished cardioprotection in the presence of little PARP activity, suggesting that the PARP activity is essential for 25-HC to exert its effect during IR injury.	25-hydroxycholesterol	4-9	poly (ADP-ribose) polymerase family, member 1	Mus musculus	104-108
31929757-9	2020	The 25-HC group abolished cardioprotection in the presence of little PARP activity, suggesting that the PARP activity is essential for 25-HC to exert its effect during IR injury.	25-hydroxycholesterol	135-140	poly (ADP-ribose) polymerase family, member 1	Mus musculus	104-108
31929757-10	2020	Our primary study indicates that 25-HC ameliorated IR injury by inhibiting the PARP activity and decreasing myocardial apoptosis, which makes it a potential therapeutic drug in IR injury of the heart.	25-hydroxycholesterol	33-38	poly (ADP-ribose) polymerase family, member 1	Mus musculus	79-83
32147789-6	2020	Here, we describe a method to test the migration of ILCs toward 7alpha,25-hydroxycholesterol, which is mediated by cell surface-expressed GPR183.	25-hydroxycholesterol	71-92	G protein-coupled receptor 183	Homo sapiens	138-144
31552533-1	2019	Cholesterol-25-hydroxylase (CH25H) is a reticulum-associated membrane protein that catalyzes the oxidation of cholesterol to 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	125-146	cholesterol 25-hydroxylase	Homo sapiens	0-26
31552533-1	2019	Cholesterol-25-hydroxylase (CH25H) is a reticulum-associated membrane protein that catalyzes the oxidation of cholesterol to 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	125-146	cholesterol 25-hydroxylase	Homo sapiens	28-33
31552533-1	2019	Cholesterol-25-hydroxylase (CH25H) is a reticulum-associated membrane protein that catalyzes the oxidation of cholesterol to 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	148-152	cholesterol 25-hydroxylase	Homo sapiens	0-26
31552533-1	2019	Cholesterol-25-hydroxylase (CH25H) is a reticulum-associated membrane protein that catalyzes the oxidation of cholesterol to 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	148-152	cholesterol 25-hydroxylase	Homo sapiens	28-33
31552533-5	2019	Treatment with the oxysterol 25-hydroxycholesterol (25HC), the product of CH25H, dramatically decreased RABV replication in 293T, BSR and N2a cells by inhibiting viral membrane penetration.	25-hydroxycholesterol	19-50	cholesterol 25-hydroxylase	Homo sapiens	74-79
31552533-5	2019	Treatment with the oxysterol 25-hydroxycholesterol (25HC), the product of CH25H, dramatically decreased RABV replication in 293T, BSR and N2a cells by inhibiting viral membrane penetration.	25-hydroxycholesterol	52-56	cholesterol 25-hydroxylase	Homo sapiens	74-79
31828178-2	2019	Lack of CYP7B1 leads to an accumulation of its oxysterol substrates, in particular 25-hydroxycholesterol and 27-hydroxycholesterol that are able to cross the blood-brain barrier and have neurotoxic properties.	25-hydroxycholesterol	83-104	cytochrome P450 family 7 subfamily B member 1	Homo sapiens	8-14
31767082-4	2019	CH25H is a multi-transmembrane and endoplasmic reticulum-related enzyme that catalyzes oxidation reaction of cholesterol to production of 25-hydroxycholesterol (25HC) and significantly inhibits the replication of several viruses.	25-hydroxycholesterol	138-159	cholesterol 25-hydroxylase	Homo sapiens	0-5
31767082-4	2019	CH25H is a multi-transmembrane and endoplasmic reticulum-related enzyme that catalyzes oxidation reaction of cholesterol to production of 25-hydroxycholesterol (25HC) and significantly inhibits the replication of several viruses.	25-hydroxycholesterol	161-165	cholesterol 25-hydroxylase	Homo sapiens	0-5
31375561-3	2019	Conversion of cholesterol to 25-hydroxycholesterol by cholesterol 25-hydroxylase (CH25H) has been shown to have broad antiviral properties.	25-hydroxycholesterol	29-50	cholesterol 25-hydroxylase	Homo sapiens	54-80
31375561-3	2019	Conversion of cholesterol to 25-hydroxycholesterol by cholesterol 25-hydroxylase (CH25H) has been shown to have broad antiviral properties.	25-hydroxycholesterol	29-50	cholesterol 25-hydroxylase	Homo sapiens	82-87
31271764-6	2019	Furthermore, OSW-1 and THEV inhibit the binding of 25-hydroxycholesterol (25-OHC) to OSBP indicating that these compounds bind at the conserved sterol ligand binding site.	25-hydroxycholesterol	51-72	oxysterol binding protein	Homo sapiens	85-89
31720079-5	2019	Mechanistically, the promotive effect of 25-HC was through up-regulation of Toll-like receptor 4 (TLR4) dependent fatty acid binding protein 4 (FABP4).	25-hydroxycholesterol	41-46	toll like receptor 4	Homo sapiens	76-96
31720079-5	2019	Mechanistically, the promotive effect of 25-HC was through up-regulation of Toll-like receptor 4 (TLR4) dependent fatty acid binding protein 4 (FABP4).	25-hydroxycholesterol	41-46	toll like receptor 4	Homo sapiens	98-102
31720079-5	2019	Mechanistically, the promotive effect of 25-HC was through up-regulation of Toll-like receptor 4 (TLR4) dependent fatty acid binding protein 4 (FABP4).	25-hydroxycholesterol	41-46	fatty acid binding protein 4	Homo sapiens	114-142
31720079-5	2019	Mechanistically, the promotive effect of 25-HC was through up-regulation of Toll-like receptor 4 (TLR4) dependent fatty acid binding protein 4 (FABP4).	25-hydroxycholesterol	41-46	fatty acid binding protein 4	Homo sapiens	144-149
31720079-6	2019	Inhibition of FABP4 hindered 25-HC-induced cells migration and metastasis.	25-hydroxycholesterol	29-34	fatty acid binding protein 4	Homo sapiens	14-19
31720079-8	2019	In summary, our study reveals the effects and mechanism of 25-HC/TLR4/FABP4 axis in promoting HCC metastasis, which provides novel avenue for therapeutic intervention against HCC progression.	25-hydroxycholesterol	59-64	toll like receptor 4	Homo sapiens	65-69
31720079-8	2019	In summary, our study reveals the effects and mechanism of 25-HC/TLR4/FABP4 axis in promoting HCC metastasis, which provides novel avenue for therapeutic intervention against HCC progression.	25-hydroxycholesterol	59-64	fatty acid binding protein 4	Homo sapiens	70-75
31271764-6	2019	Furthermore, OSW-1 and THEV inhibit the binding of 25-hydroxycholesterol (25-OHC) to OSBP indicating that these compounds bind at the conserved sterol ligand binding site.	25-hydroxycholesterol	74-80	oxysterol binding protein	Homo sapiens	85-89
31220227-4	2019	The enzyme cholesterol 25-hydroxylase [CH25H] converts cholesterol to 25-hydroxycholesterol [25-HC], which modulates immune responses and oxidative stress.	25-hydroxycholesterol	70-91	cholesterol 25-hydroxylase	Homo sapiens	11-37
31341055-2	2019	Previous studies from our and other groups showed that cholesterol 25-hydroxylase (CH25H), a multi-transmembrane endoplasmic reticulum-associated enzyme, catalyzes the production of 25-hydroxycholesterol (25HC) and inhibits PRRS virus (PRRSV) replication.	25-hydroxycholesterol	182-203	cholesterol 25-hydroxylase	Homo sapiens	83-88
31341055-10	2019	Taken together, our results and those of previous studies of the anti-PRRSV effects of CH25H highlight the complex interplay between PRRSV and pCH25H.IMPORTANCECholesterol 25-hydroxylase (CH25H) has received significant attention due to its broad antiviral activity, which it mediates by catalyzing the production of 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	317-338	cholesterol 25-hydroxylase	Homo sapiens	87-92
31341055-10	2019	Taken together, our results and those of previous studies of the anti-PRRSV effects of CH25H highlight the complex interplay between PRRSV and pCH25H.IMPORTANCECholesterol 25-hydroxylase (CH25H) has received significant attention due to its broad antiviral activity, which it mediates by catalyzing the production of 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	317-338	cholesterol 25-hydroxylase	Homo sapiens	144-149
31220227-4	2019	The enzyme cholesterol 25-hydroxylase [CH25H] converts cholesterol to 25-hydroxycholesterol [25-HC], which modulates immune responses and oxidative stress.	25-hydroxycholesterol	70-91	cholesterol 25-hydroxylase	Homo sapiens	39-44
30813555-10	2019	25-Hydroxycholesterol (25-HC) inhibits lipid transport by high affinity binding OSBP.	25-hydroxycholesterol	0-21	oxysterol binding protein	Homo sapiens	80-84
30902677-5	2019	The present study addresses a function of 11beta-HSD1 in the enzymatic generation of 7beta,25-dihydroxycholesterol (7beta25OHC) from 7-keto,25-hydroxycholesterol (7k25OHC) and tested whether 11beta-HSD2 is able to catalyze the reverse reaction.	25-hydroxycholesterol	140-161	hydroxysteroid 11-beta dehydrogenase 1	Homo sapiens	42-53
30902677-5	2019	The present study addresses a function of 11beta-HSD1 in the enzymatic generation of 7beta,25-dihydroxycholesterol (7beta25OHC) from 7-keto,25-hydroxycholesterol (7k25OHC) and tested whether 11beta-HSD2 is able to catalyze the reverse reaction.	25-hydroxycholesterol	140-161	hydroxysteroid 11-beta dehydrogenase 2	Homo sapiens	191-202
30710743-2	2019	The mitochondrial CYP27A1 initiated pathway of cholesterol metabolism (acidic pathway) is known to synthesize two well-described vital regulators of cholesterol/lipid homeostasis, (25R)-26-hydroxycholesterol (26HC) and 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	219-240	cytochrome P450 family 27 subfamily A member 1	Homo sapiens	18-25
30592645-5	2019	We show that the SREBP-2 pathway is crucial for the intoxication process of toxins A and B by using pharmacological inhibitors (PF-429242, 25-hydroxycholesterol) and cells that are specifically deficient in SREBP-2 pathway signaling.	25-hydroxycholesterol	139-160	sterol regulatory element binding transcription factor 2	Rattus norvegicus	17-24
30955800-0	2019	Cholesterol 25-hydroxylase negatively regulates porcine intestinal coronavirus replication by the production of 25-hydroxycholesterol.	25-hydroxycholesterol	112-133	cholesterol 25-hydroxylase	Homo sapiens	0-26
30955800-1	2019	Cholesterol 25-hydroxylase (CH25H) has been shown lately to be a host restriction factor that encodes an enzyme, which catalyzes the oxidized form of cholesterol to 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	165-186	cholesterol 25-hydroxylase	Homo sapiens	0-26
30955800-1	2019	Cholesterol 25-hydroxylase (CH25H) has been shown lately to be a host restriction factor that encodes an enzyme, which catalyzes the oxidized form of cholesterol to 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	165-186	cholesterol 25-hydroxylase	Homo sapiens	28-33
30955800-1	2019	Cholesterol 25-hydroxylase (CH25H) has been shown lately to be a host restriction factor that encodes an enzyme, which catalyzes the oxidized form of cholesterol to 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	188-192	cholesterol 25-hydroxylase	Homo sapiens	0-26
30955800-1	2019	Cholesterol 25-hydroxylase (CH25H) has been shown lately to be a host restriction factor that encodes an enzyme, which catalyzes the oxidized form of cholesterol to 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	188-192	cholesterol 25-hydroxylase	Homo sapiens	28-33
30391516-0	2019	25-hydroxycholesterol down-regulates oxysterol binding protein like 2 (OSBPL2) via the p53/SREBF2/NFYA signaling pathway.	25-hydroxycholesterol	0-21	tumor protein p53	Homo sapiens	87-90
30391516-0	2019	25-hydroxycholesterol down-regulates oxysterol binding protein like 2 (OSBPL2) via the p53/SREBF2/NFYA signaling pathway.	25-hydroxycholesterol	0-21	sterol regulatory element binding transcription factor 2	Homo sapiens	91-97
30391516-0	2019	25-hydroxycholesterol down-regulates oxysterol binding protein like 2 (OSBPL2) via the p53/SREBF2/NFYA signaling pathway.	25-hydroxycholesterol	0-21	nuclear transcription factor Y subunit alpha	Homo sapiens	98-102
30391516-2	2019	Previous studies have shown that depression of OSBPL2 significantly increases the level of cellular 25-hydroxycholesterol (25-OHC) which regulates the expression of lipid-metabolism-related genes.	25-hydroxycholesterol	100-121	oxysterol binding protein like 2	Homo sapiens	47-53
30391516-2	2019	Previous studies have shown that depression of OSBPL2 significantly increases the level of cellular 25-hydroxycholesterol (25-OHC) which regulates the expression of lipid-metabolism-related genes.	25-hydroxycholesterol	123-129	oxysterol binding protein like 2	Homo sapiens	47-53
30391516-5	2019	Using dual-luciferase reporter assay, we found a decrease of nuclear transcription factor Y subunit alpha (NFYA) bound with OSBPL2 promoter when HeLa cells were treated with 25-OHC.	25-hydroxycholesterol	174-180	nuclear transcription factor Y subunit alpha	Homo sapiens	61-105
30391516-5	2019	Using dual-luciferase reporter assay, we found a decrease of nuclear transcription factor Y subunit alpha (NFYA) bound with OSBPL2 promoter when HeLa cells were treated with 25-OHC.	25-hydroxycholesterol	174-180	nuclear transcription factor Y subunit alpha	Homo sapiens	107-111
30391516-5	2019	Using dual-luciferase reporter assay, we found a decrease of nuclear transcription factor Y subunit alpha (NFYA) bound with OSBPL2 promoter when HeLa cells were treated with 25-OHC.	25-hydroxycholesterol	174-180	oxysterol binding protein like 2	Homo sapiens	124-130
30391516-8	2019	25-OHC will accumulate over time in OSBPL2 knockdown cells.	25-hydroxycholesterol	0-6	oxysterol binding protein like 2	Homo sapiens	36-42
31039009-10	2019	Finally, the predisposition to inflammation sensitivity displayed by Myd88DeltaHep mice may be caused by the accumulation of 25-hydroxycholesterol, an oxysterol linked to inflammatory response and metabolic disorders.	25-hydroxycholesterol	125-146	myeloid differentiation primary response gene 88	Mus musculus	69-82
30931941-3	2019	Here we show that a lipid, oxysterol 25-hydroxycholesterol (25HC), directly binds to alpha5beta1 and alphavbeta3 integrins to activate integrin-focal adhesion kinase (FAK) signaling.	25-hydroxycholesterol	60-64	protein tyrosine kinase 2	Homo sapiens	135-165
30931941-3	2019	Here we show that a lipid, oxysterol 25-hydroxycholesterol (25HC), directly binds to alpha5beta1 and alphavbeta3 integrins to activate integrin-focal adhesion kinase (FAK) signaling.	25-hydroxycholesterol	60-64	protein tyrosine kinase 2	Homo sapiens	167-170
30894855-1	2019	Cholesterol 25-hydroxylase (CH25H) is an interferon (IFN)-induced gene that catalyzes the oxidation of cholesterol to 25-hydroxycholesterol (25HC), which exerts broad-spectrum antiviral function.	25-hydroxycholesterol	118-139	cholesterol 25-hydroxylase	Homo sapiens	0-26
30894855-1	2019	Cholesterol 25-hydroxylase (CH25H) is an interferon (IFN)-induced gene that catalyzes the oxidation of cholesterol to 25-hydroxycholesterol (25HC), which exerts broad-spectrum antiviral function.	25-hydroxycholesterol	118-139	cholesterol 25-hydroxylase	Homo sapiens	28-33
30894855-1	2019	Cholesterol 25-hydroxylase (CH25H) is an interferon (IFN)-induced gene that catalyzes the oxidation of cholesterol to 25-hydroxycholesterol (25HC), which exerts broad-spectrum antiviral function.	25-hydroxycholesterol	141-145	cholesterol 25-hydroxylase	Homo sapiens	0-26
30894855-1	2019	Cholesterol 25-hydroxylase (CH25H) is an interferon (IFN)-induced gene that catalyzes the oxidation of cholesterol to 25-hydroxycholesterol (25HC), which exerts broad-spectrum antiviral function.	25-hydroxycholesterol	141-145	cholesterol 25-hydroxylase	Homo sapiens	28-33
30813555-10	2019	25-Hydroxycholesterol (25-HC) inhibits lipid transport by high affinity binding OSBP.	25-hydroxycholesterol	23-28	oxysterol binding protein	Homo sapiens	80-84
30700717-4	2019	Inhibition of the cholesterol biosynthesis pathway with atorvastatin or 25-hydroxycholesterol during switching from IFNgamma+ to IL-10+ shows a specific block in immune resolution, defined as a significant decrease in IL-10 expression.	25-hydroxycholesterol	72-93	interferon gamma	Homo sapiens	116-124
30700717-4	2019	Inhibition of the cholesterol biosynthesis pathway with atorvastatin or 25-hydroxycholesterol during switching from IFNgamma+ to IL-10+ shows a specific block in immune resolution, defined as a significant decrease in IL-10 expression.	25-hydroxycholesterol	72-93	interleukin 10	Homo sapiens	129-134
30700717-4	2019	Inhibition of the cholesterol biosynthesis pathway with atorvastatin or 25-hydroxycholesterol during switching from IFNgamma+ to IL-10+ shows a specific block in immune resolution, defined as a significant decrease in IL-10 expression.	25-hydroxycholesterol	72-93	interleukin 10	Homo sapiens	218-223
30700717-5	2019	Mechanistically, the master transcriptional regulator of IL10 in T cells, c-Maf, is significantly decreased by physiological levels of 25-hydroxycholesterol.	25-hydroxycholesterol	135-156	interleukin 10	Homo sapiens	57-61
30700717-5	2019	Mechanistically, the master transcriptional regulator of IL10 in T cells, c-Maf, is significantly decreased by physiological levels of 25-hydroxycholesterol.	25-hydroxycholesterol	135-156	MAF bZIP transcription factor	Homo sapiens	74-79
30645975-3	2019	CH25H produces 25-hydroxycholesterol, which inhibited TEV uptake.	25-hydroxycholesterol	15-36	cholesterol 25-hydroxylase	Homo sapiens	0-5
30653529-2	2019	In this study, we described novel preferential bindings of 25-hydroxycholesterol (25HOCh) to CRP1-7 compared with other lipids and explored the antiviral effects of both 25HOCh and CRP1-7 against spring viremia carp virus (SVCV) infection in zebrafish.	25-hydroxycholesterol	59-80	cysteine rich protein 3	Homo sapiens	93-99
30309895-1	2018	Production of 25-hydroxycholesterol (25HC), a potent inhibitor of viral infection, is catalyzed by cholesterol 25-hydroxylase (CH25H).	25-hydroxycholesterol	14-35	cholesterol 25-hydroxylase	Homo sapiens	99-125
30391516-0	2019	25-hydroxycholesterol down-regulates oxysterol binding protein like 2 (OSBPL2) via the p53/SREBF2/NFYA signaling pathway.	25-hydroxycholesterol	0-21	oxysterol binding protein like 2	Homo sapiens	37-69
30391516-0	2019	25-hydroxycholesterol down-regulates oxysterol binding protein like 2 (OSBPL2) via the p53/SREBF2/NFYA signaling pathway.	25-hydroxycholesterol	0-21	oxysterol binding protein like 2	Homo sapiens	71-77
30309895-1	2018	Production of 25-hydroxycholesterol (25HC), a potent inhibitor of viral infection, is catalyzed by cholesterol 25-hydroxylase (CH25H).	25-hydroxycholesterol	14-35	cholesterol 25-hydroxylase	Homo sapiens	127-132
30309895-1	2018	Production of 25-hydroxycholesterol (25HC), a potent inhibitor of viral infection, is catalyzed by cholesterol 25-hydroxylase (CH25H).	25-hydroxycholesterol	37-41	cholesterol 25-hydroxylase	Homo sapiens	99-125
30309895-1	2018	Production of 25-hydroxycholesterol (25HC), a potent inhibitor of viral infection, is catalyzed by cholesterol 25-hydroxylase (CH25H).	25-hydroxycholesterol	37-41	cholesterol 25-hydroxylase	Homo sapiens	127-132
29879417-2	2018	In this study, we used dual polarization interferometry (DPI), a label-free surface analytical technique, to characterize the interaction of recombinant, purified OSBP as it flows over immobilized dioleoyl-phosphatidylcholine (DOPC) bilayers containing PI(4)P, cholesterol or 25-hydroxycholesterol.	25-hydroxycholesterol	276-297	oxysterol binding protein	Homo sapiens	163-167
30524435-2	2018	Previously, we reported that cholesterol-25-hydroxylase (CH25H) and its metabolite 25-hydroxycholesterol (25-HC) had a broad antiviral activity in inhibiting Zika, Ebola, and HIV-1 infection.	25-hydroxycholesterol	83-104	cholesterol 25-hydroxylase	Homo sapiens	29-55
30524435-2	2018	Previously, we reported that cholesterol-25-hydroxylase (CH25H) and its metabolite 25-hydroxycholesterol (25-HC) had a broad antiviral activity in inhibiting Zika, Ebola, and HIV-1 infection.	25-hydroxycholesterol	83-104	cholesterol 25-hydroxylase	Homo sapiens	57-62
30524435-2	2018	Previously, we reported that cholesterol-25-hydroxylase (CH25H) and its metabolite 25-hydroxycholesterol (25-HC) had a broad antiviral activity in inhibiting Zika, Ebola, and HIV-1 infection.	25-hydroxycholesterol	106-111	cholesterol 25-hydroxylase	Homo sapiens	29-55
30524435-2	2018	Previously, we reported that cholesterol-25-hydroxylase (CH25H) and its metabolite 25-hydroxycholesterol (25-HC) had a broad antiviral activity in inhibiting Zika, Ebola, and HIV-1 infection.	25-hydroxycholesterol	106-111	cholesterol 25-hydroxylase	Homo sapiens	57-62
29930082-3	2018	Characterization of ORP4L lipid and VAP binding mutants indicated an indirect mechanism for translocation to ER-Golgi contact sites in response to 25-hydroxycholesterol that was dependent on OSBP and PI(4)P.	25-hydroxycholesterol	147-168	oxysterol binding protein	Homo sapiens	191-195
29689289-1	2018	Dihydroxycholesterols such as 7alpha,25-dihydroxysterols (7alpha,25-OHC) and 7alpha,27-OHC are generated from cholesterol by the enzymes CH25H, CYP7B1 and CYP27A1 in steady state but also in the context of inflammation.	25-hydroxycholesterol	65-71	cholesterol 25-hydroxylase	Homo sapiens	137-142
29689289-1	2018	Dihydroxycholesterols such as 7alpha,25-dihydroxysterols (7alpha,25-OHC) and 7alpha,27-OHC are generated from cholesterol by the enzymes CH25H, CYP7B1 and CYP27A1 in steady state but also in the context of inflammation.	25-hydroxycholesterol	65-71	cytochrome P450 family 7 subfamily B member 1	Homo sapiens	144-150
29689289-1	2018	Dihydroxycholesterols such as 7alpha,25-dihydroxysterols (7alpha,25-OHC) and 7alpha,27-OHC are generated from cholesterol by the enzymes CH25H, CYP7B1 and CYP27A1 in steady state but also in the context of inflammation.	25-hydroxycholesterol	65-71	cytochrome P450 family 27 subfamily A member 1	Homo sapiens	155-162
30091835-0	2018	25-Hydroxycholesterol protects against acute lung injury via targeting MD-2.	25-hydroxycholesterol	0-21	lymphocyte antigen 96	Mus musculus	71-75
30091835-10	2018	In summary, this study demonstrates that 25HC could inhibit the overwhelming inflammatory response through MD-2 interaction, which suppresses Akt/NF-kappaB signalling pathway.	25-hydroxycholesterol	41-45	lymphocyte antigen 96	Mus musculus	107-111
30091835-10	2018	In summary, this study demonstrates that 25HC could inhibit the overwhelming inflammatory response through MD-2 interaction, which suppresses Akt/NF-kappaB signalling pathway.	25-hydroxycholesterol	41-45	thymoma viral proto-oncogene 1	Mus musculus	142-145
30091835-10	2018	In summary, this study demonstrates that 25HC could inhibit the overwhelming inflammatory response through MD-2 interaction, which suppresses Akt/NF-kappaB signalling pathway.	25-hydroxycholesterol	41-45	nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105	Mus musculus	146-155
29899427-11	2018	We conclude that Tg6F alters levels of specific oxidized lipids and 25-OHC to modulate Notch pathways and Spp1, which alter small intestine immune cells, leading to similar changes in lung that reduce tumor burden.	25-hydroxycholesterol	68-74	notch 1	Mus musculus	87-92
29949760-4	2018	Here, we show that GPR183, a chemotactic receptor expressed on murine and human ILC3s, regulates ILC3 migration toward its ligand 7alpha,25-dihydroxycholesterol (7alpha,25-OHC) in vitro, and GPR183 deficiency in vivo leads to a disorganized distribution of ILC3s in mesenteric lymph nodes and decreased ILC3 accumulation in the intestine.	25-hydroxycholesterol	169-175	G protein-coupled receptor 183	Mus musculus	19-25
29343433-3	2018	Here, we show that ILC3s with a lymphoid-tissue-inducer (LTi) phenotype expressed G-protein-coupled receptor 183 (GPR183) and migrated to its oxysterol ligand 7alpha,25-hydroxycholesterol (7alpha,25-OHC).	25-hydroxycholesterol	196-202	G protein-coupled receptor 183	Mus musculus	114-120
29523554-3	2018	METHODS AND RESULTS: We show that LIPA inhibition causes a defective efferocytic response because of impaired generation of 25-hydroxycholesterol and 27-hydroxycholesterol.	25-hydroxycholesterol	124-145	lysosomal acid lipase A	Mus musculus	34-38
29523554-4	2018	Reduced synthesis of 25-hydroxycholesterol after LIPA inhibition contributed to defective mitochondria-associated membrane leading to mitochondrial oxidative stress-induced NLRP3 (NOD-like receptor family, pyrin domain containing) inflammasome activation and caspase-1-dependent Rac1 (Ras-related C3 botulinum toxin substrate 1) degradation.	25-hydroxycholesterol	21-42	lysosomal acid lipase A	Mus musculus	49-53
29523554-4	2018	Reduced synthesis of 25-hydroxycholesterol after LIPA inhibition contributed to defective mitochondria-associated membrane leading to mitochondrial oxidative stress-induced NLRP3 (NOD-like receptor family, pyrin domain containing) inflammasome activation and caspase-1-dependent Rac1 (Ras-related C3 botulinum toxin substrate 1) degradation.	25-hydroxycholesterol	21-42	NLR family, pyrin domain containing 3	Mus musculus	173-178
29523554-4	2018	Reduced synthesis of 25-hydroxycholesterol after LIPA inhibition contributed to defective mitochondria-associated membrane leading to mitochondrial oxidative stress-induced NLRP3 (NOD-like receptor family, pyrin domain containing) inflammasome activation and caspase-1-dependent Rac1 (Ras-related C3 botulinum toxin substrate 1) degradation.	25-hydroxycholesterol	21-42	caspase 1	Mus musculus	259-268
29523554-4	2018	Reduced synthesis of 25-hydroxycholesterol after LIPA inhibition contributed to defective mitochondria-associated membrane leading to mitochondrial oxidative stress-induced NLRP3 (NOD-like receptor family, pyrin domain containing) inflammasome activation and caspase-1-dependent Rac1 (Ras-related C3 botulinum toxin substrate 1) degradation.	25-hydroxycholesterol	21-42	Rac family small GTPase 1	Mus musculus	279-283
29523554-4	2018	Reduced synthesis of 25-hydroxycholesterol after LIPA inhibition contributed to defective mitochondria-associated membrane leading to mitochondrial oxidative stress-induced NLRP3 (NOD-like receptor family, pyrin domain containing) inflammasome activation and caspase-1-dependent Rac1 (Ras-related C3 botulinum toxin substrate 1) degradation.	25-hydroxycholesterol	21-42	Rac family small GTPase 1	Mus musculus	285-327
29514919-3	2018	After treating cells with 25-hydroxycholesterol to induce OSBP relocation from the cytoplasm to the TGN, REs accumulated around the TGN area, but this accumulation was diminished in RELCH- or OSBP-depleted cells.	25-hydroxycholesterol	26-47	oxysterol binding protein	Homo sapiens	58-62
29514919-3	2018	After treating cells with 25-hydroxycholesterol to induce OSBP relocation from the cytoplasm to the TGN, REs accumulated around the TGN area, but this accumulation was diminished in RELCH- or OSBP-depleted cells.	25-hydroxycholesterol	26-47	oxysterol binding protein	Homo sapiens	192-196
29298812-0	2018	25-Hydroxycholesterol activates the expression of cholesterol 25-hydroxylase in an LXR-dependent mechanism.	25-hydroxycholesterol	0-21	cholesterol 25-hydroxylase	Homo sapiens	50-76
29298812-1	2018	Cholesterol 25-hydroxylase (CH25H) catalyzes the production of 25-hydroxycholesterol (25-HC), an oxysterol that can play an important role in different biological processes.	25-hydroxycholesterol	63-84	cholesterol 25-hydroxylase	Homo sapiens	0-26
29298812-1	2018	Cholesterol 25-hydroxylase (CH25H) catalyzes the production of 25-hydroxycholesterol (25-HC), an oxysterol that can play an important role in different biological processes.	25-hydroxycholesterol	63-84	cholesterol 25-hydroxylase	Homo sapiens	28-33
29298812-1	2018	Cholesterol 25-hydroxylase (CH25H) catalyzes the production of 25-hydroxycholesterol (25-HC), an oxysterol that can play an important role in different biological processes.	25-hydroxycholesterol	86-91	cholesterol 25-hydroxylase	Homo sapiens	0-26
29298812-1	2018	Cholesterol 25-hydroxylase (CH25H) catalyzes the production of 25-hydroxycholesterol (25-HC), an oxysterol that can play an important role in different biological processes.	25-hydroxycholesterol	86-91	cholesterol 25-hydroxylase	Homo sapiens	28-33
29296233-7	2017	Conversely, SREBP-2 suppression with specific siRNA or the addition of cholesterol/25-hydroxycholesterol to cell culture medium reduces transcriptional activity of SND1 promoter and SND1 mRNA abundance.	25-hydroxycholesterol	83-104	staphylococcal nuclease and tudor domain containing 1	Homo sapiens	164-168
29296233-7	2017	Conversely, SREBP-2 suppression with specific siRNA or the addition of cholesterol/25-hydroxycholesterol to cell culture medium reduces transcriptional activity of SND1 promoter and SND1 mRNA abundance.	25-hydroxycholesterol	83-104	staphylococcal nuclease and tudor domain containing 1	Homo sapiens	182-186
29033131-3	2017	Here, we demonstrate that production of 25-hydroxycholesterol (25-HC) by macrophages is required to prevent inflammasome activation by the DNA sensor protein absent in melanoma 2 (AIM2).	25-hydroxycholesterol	40-61	absent in melanoma 2	Homo sapiens	158-178
29033131-3	2017	Here, we demonstrate that production of 25-hydroxycholesterol (25-HC) by macrophages is required to prevent inflammasome activation by the DNA sensor protein absent in melanoma 2 (AIM2).	25-hydroxycholesterol	40-61	absent in melanoma 2	Homo sapiens	180-184
28870504-2	2017	We recently have shown that 25-hydroxycholesterol found in atherosclerotic lesions could impair endothelial function and vasodilation by uncoupling and inhibiting endothelial nitric oxide synthase (eNOS).	25-hydroxycholesterol	28-49	nitric oxide synthase 3	Homo sapiens	163-196
28918367-4	2017	Since 25-hydroxycholesterol (25-HC), a metabolite of cholesterol, can suppress IL-1beta production, thus reducing inflammation, we evaluated the effect of 25-HC in an in vitro model of mevalonate pathway alteration, obtained using Lovastatin.	25-hydroxycholesterol	6-27	interleukin 1 beta	Homo sapiens	79-87
28918367-4	2017	Since 25-hydroxycholesterol (25-HC), a metabolite of cholesterol, can suppress IL-1beta production, thus reducing inflammation, we evaluated the effect of 25-HC in an in vitro model of mevalonate pathway alteration, obtained using Lovastatin.	25-hydroxycholesterol	29-34	interleukin 1 beta	Homo sapiens	79-87
28870504-2	2017	We recently have shown that 25-hydroxycholesterol found in atherosclerotic lesions could impair endothelial function and vasodilation by uncoupling and inhibiting endothelial nitric oxide synthase (eNOS).	25-hydroxycholesterol	28-49	nitric oxide synthase 3	Homo sapiens	198-202
28724759-1	2017	Cholesterol 25-hydroxylase (CH25H) has recently been identified as a host restriction factor that exerts antiviral effects by catalyzing the production of 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	155-176	cholesterol 25-hydroxylase	Homo sapiens	0-26
28599978-0	2017	25-Hydroxycholesterol induces both P2X7-dependent pyroptosis and caspase-dependent apoptosis in human skin model: New insights into degenerative pathways.	25-hydroxycholesterol	0-21	purinergic receptor P2X 7	Homo sapiens	35-39
29103685-2	2017	Cholesterol 25-hydroxylase (CH25H) is an enzyme that catalyzes oxidation of cholesterol to 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	91-112	cholesterol 25-hydroxylase	Homo sapiens	0-26
29103685-2	2017	Cholesterol 25-hydroxylase (CH25H) is an enzyme that catalyzes oxidation of cholesterol to 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	91-112	cholesterol 25-hydroxylase	Homo sapiens	28-33
29103685-2	2017	Cholesterol 25-hydroxylase (CH25H) is an enzyme that catalyzes oxidation of cholesterol to 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	114-118	cholesterol 25-hydroxylase	Homo sapiens	0-26
29103685-2	2017	Cholesterol 25-hydroxylase (CH25H) is an enzyme that catalyzes oxidation of cholesterol to 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	114-118	cholesterol 25-hydroxylase	Homo sapiens	28-33
28724759-1	2017	Cholesterol 25-hydroxylase (CH25H) has recently been identified as a host restriction factor that exerts antiviral effects by catalyzing the production of 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	155-176	cholesterol 25-hydroxylase	Homo sapiens	28-33
28724759-14	2017	CH25H exerts its anti-PRRSV effect not only via the production of 25HC to inhibit viral penetration but also by degrading viral protein through the ubiquitin-proteasome pathway, suggesting that CH25H is a candidate for the development of antiviral therapeutics.	25-hydroxycholesterol	66-70	cholesterol 25-hydroxylase	Homo sapiens	0-5
28724759-14	2017	CH25H exerts its anti-PRRSV effect not only via the production of 25HC to inhibit viral penetration but also by degrading viral protein through the ubiquitin-proteasome pathway, suggesting that CH25H is a candidate for the development of antiviral therapeutics.	25-hydroxycholesterol	66-70	cholesterol 25-hydroxylase	Homo sapiens	194-199
28775317-5	2017	25-hydroxycholesterol (25-OH), an inhibitor of FASN expression, also inhibited HIF target gene expression in cultured cells and in mouse liver.	25-hydroxycholesterol	0-21	fatty acid synthase	Mus musculus	47-51
28775317-5	2017	25-hydroxycholesterol (25-OH), an inhibitor of FASN expression, also inhibited HIF target gene expression in cultured cells and in mouse liver.	25-hydroxycholesterol	23-28	fatty acid synthase	Mus musculus	47-51
28471037-4	2017	OSBP and Sac1 co-localized at apparent ER-Golgi contact sites in response to 25-hydroxycholesterol (25OH), cholesterol depletion and p38 MAPK inhibitors.	25-hydroxycholesterol	77-98	oxysterol binding protein	Homo sapiens	0-4
28592401-2	2017	WD also increased the expression of cholesterol 25-hydroxylase (CH25H) as measured by RT-qPCR (P &lt; 0.0001), IHC (P = 0.0019), and ELISA (P &lt; 0.0001), resulting in increased levels of 25-hydroxycholesterol (25-OHC) in jejunum as determined by LC-MS/MS (P &lt; 0.0001).	25-hydroxycholesterol	189-210	cholesterol 25-hydroxylase	Mus musculus	36-62
28592401-2	2017	WD also increased the expression of cholesterol 25-hydroxylase (CH25H) as measured by RT-qPCR (P &lt; 0.0001), IHC (P = 0.0019), and ELISA (P &lt; 0.0001), resulting in increased levels of 25-hydroxycholesterol (25-OHC) in jejunum as determined by LC-MS/MS (P &lt; 0.0001).	25-hydroxycholesterol	189-210	cholesterol 25-hydroxylase	Mus musculus	64-69
28592401-2	2017	WD also increased the expression of cholesterol 25-hydroxylase (CH25H) as measured by RT-qPCR (P &lt; 0.0001), IHC (P = 0.0019), and ELISA (P &lt; 0.0001), resulting in increased levels of 25-hydroxycholesterol (25-OHC) in jejunum as determined by LC-MS/MS (P &lt; 0.0001).	25-hydroxycholesterol	212-218	cholesterol 25-hydroxylase	Mus musculus	36-62
28592401-2	2017	WD also increased the expression of cholesterol 25-hydroxylase (CH25H) as measured by RT-qPCR (P &lt; 0.0001), IHC (P = 0.0019), and ELISA (P &lt; 0.0001), resulting in increased levels of 25-hydroxycholesterol (25-OHC) in jejunum as determined by LC-MS/MS (P &lt; 0.0001).	25-hydroxycholesterol	212-218	cholesterol 25-hydroxylase	Mus musculus	64-69
28471037-4	2017	OSBP and Sac1 co-localized at apparent ER-Golgi contact sites in response to 25-hydroxycholesterol (25OH), cholesterol depletion and p38 MAPK inhibitors.	25-hydroxycholesterol	77-98	SAC1 like phosphatidylinositide phosphatase	Homo sapiens	9-13
28471037-4	2017	OSBP and Sac1 co-localized at apparent ER-Golgi contact sites in response to 25-hydroxycholesterol (25OH), cholesterol depletion and p38 MAPK inhibitors.	25-hydroxycholesterol	100-104	oxysterol binding protein	Homo sapiens	0-4
28471037-4	2017	OSBP and Sac1 co-localized at apparent ER-Golgi contact sites in response to 25-hydroxycholesterol (25OH), cholesterol depletion and p38 MAPK inhibitors.	25-hydroxycholesterol	100-104	SAC1 like phosphatidylinositide phosphatase	Homo sapiens	9-13
28631596-1	2017	Cholesterol 25-hydroxylase (CH25H) catalyses the production of 25-hydroxycholesterol (25HC) from cholesterol by adding a second hydroxyl group at position 25.	25-hydroxycholesterol	63-84	cholesterol 25-hydroxylase	Homo sapiens	0-26
28631596-1	2017	Cholesterol 25-hydroxylase (CH25H) catalyses the production of 25-hydroxycholesterol (25HC) from cholesterol by adding a second hydroxyl group at position 25.	25-hydroxycholesterol	63-84	cholesterol 25-hydroxylase	Homo sapiens	28-33
28631596-1	2017	Cholesterol 25-hydroxylase (CH25H) catalyses the production of 25-hydroxycholesterol (25HC) from cholesterol by adding a second hydroxyl group at position 25.	25-hydroxycholesterol	86-90	cholesterol 25-hydroxylase	Homo sapiens	0-26
28631596-1	2017	Cholesterol 25-hydroxylase (CH25H) catalyses the production of 25-hydroxycholesterol (25HC) from cholesterol by adding a second hydroxyl group at position 25.	25-hydroxycholesterol	86-90	cholesterol 25-hydroxylase	Homo sapiens	28-33
28060747-4	2017	In the motor neuron-like cell line (NSC34) with the human mutant G93A superoxide dismutase 1 gene (mSOD1-G93A), 25-hydroxycholesterol induced motor neuronal death/ apoptosis via glycogen synthase kinase-3beta and liver X receptor pathways; riluzole treatment attenuated these effects.	25-hydroxycholesterol	112-133	superoxide dismutase 1	Homo sapiens	70-92
28257846-0	2017	25-hydroxycholesterol promotes RANKL-induced osteoclastogenesis through coordinating NFATc1 and Sp1 complex in the transcription of miR-139-5p.	25-hydroxycholesterol	0-21	tumor necrosis factor (ligand) superfamily, member 11	Mus musculus	31-36
28257846-0	2017	25-hydroxycholesterol promotes RANKL-induced osteoclastogenesis through coordinating NFATc1 and Sp1 complex in the transcription of miR-139-5p.	25-hydroxycholesterol	0-21	nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1	Mus musculus	85-91
26851362-12	2017	In a previous work triple-knockout mice deficient in the biosynthesis of 24S-hydroxycholesterol, 25-hydroxycholesterol and 27OH were shown to have impaired response to dietary cholesterol, suggesting side-chain oxidized oxysterols to be mediators in cholesterol-induced effects on LXR target genes at a transcriptional level (Chen W. et al., Cell Metab.	25-hydroxycholesterol	97-118	nuclear receptor subfamily 1, group H, member 3	Mus musculus	281-284
28060747-4	2017	In the motor neuron-like cell line (NSC34) with the human mutant G93A superoxide dismutase 1 gene (mSOD1-G93A), 25-hydroxycholesterol induced motor neuronal death/ apoptosis via glycogen synthase kinase-3beta and liver X receptor pathways; riluzole treatment attenuated these effects.	25-hydroxycholesterol	112-133	superoxide dismutase 1, soluble	Mus musculus	99-104
28060747-4	2017	In the motor neuron-like cell line (NSC34) with the human mutant G93A superoxide dismutase 1 gene (mSOD1-G93A), 25-hydroxycholesterol induced motor neuronal death/ apoptosis via glycogen synthase kinase-3beta and liver X receptor pathways; riluzole treatment attenuated these effects.	25-hydroxycholesterol	112-133	glycogen synthase kinase 3 beta	Homo sapiens	178-208
28060747-5	2017	The expressions of enzymes that synthesize 25-hydroxycholesterol were significantly increased in the brains of early symptomatic mSOD1G93A mice.	25-hydroxycholesterol	43-64	superoxide dismutase 1, soluble	Mus musculus	129-134
28161398-0	2017	Angiotensin-II-induced Muscle Wasting is Mediated by 25-Hydroxycholesterol via GSK3beta Signaling Pathway.	25-hydroxycholesterol	53-74	glycogen synthase kinase 3 beta	Mus musculus	79-87
28161398-6	2017	Intraperitoneal injection of 25-hydroxycholesterol (25-OHC), the product of Ch25h, resulted in muscle loss in C57BL/6 mice, accompanied by increased expression of atrogin-1, MuRF1 and suppression of IGF-1/Akt signaling pathway.	25-hydroxycholesterol	29-50	cholesterol 25-hydroxylase	Mus musculus	76-81
28161398-6	2017	Intraperitoneal injection of 25-hydroxycholesterol (25-OHC), the product of Ch25h, resulted in muscle loss in C57BL/6 mice, accompanied by increased expression of atrogin-1, MuRF1 and suppression of IGF-1/Akt signaling pathway.	25-hydroxycholesterol	29-50	F-box protein 32	Mus musculus	163-172
28161398-6	2017	Intraperitoneal injection of 25-hydroxycholesterol (25-OHC), the product of Ch25h, resulted in muscle loss in C57BL/6 mice, accompanied by increased expression of atrogin-1, MuRF1 and suppression of IGF-1/Akt signaling pathway.	25-hydroxycholesterol	29-50	tripartite motif-containing 63	Mus musculus	174-179
28161398-6	2017	Intraperitoneal injection of 25-hydroxycholesterol (25-OHC), the product of Ch25h, resulted in muscle loss in C57BL/6 mice, accompanied by increased expression of atrogin-1, MuRF1 and suppression of IGF-1/Akt signaling pathway.	25-hydroxycholesterol	29-50	insulin-like growth factor 1	Mus musculus	199-204
28161398-6	2017	Intraperitoneal injection of 25-hydroxycholesterol (25-OHC), the product of Ch25h, resulted in muscle loss in C57BL/6 mice, accompanied by increased expression of atrogin-1, MuRF1 and suppression of IGF-1/Akt signaling pathway.	25-hydroxycholesterol	29-50	thymoma viral proto-oncogene 1	Mus musculus	205-208
28052250-2	2017	We previously showed that memory CD4+ T lymphocytes migrate specifically in response to 7alpha,25-dihydroxycholesterol (7alpha,25-OHC) via EBI2 in the MS murine model experimental autoimmune encephalomyelitis.	25-hydroxycholesterol	127-133	CD4 molecule	Homo sapiens	33-36
28147280-3	2017	CH25H and CYP7B1 hydroxylate cholesterol to 7alpha,25-OHC.	25-hydroxycholesterol	51-57	cholesterol 25-hydroxylase	Homo sapiens	0-5
28147280-3	2017	CH25H and CYP7B1 hydroxylate cholesterol to 7alpha,25-OHC.	25-hydroxycholesterol	51-57	cytochrome P450 family 7 subfamily B member 1	Homo sapiens	10-16
28052250-2	2017	We previously showed that memory CD4+ T lymphocytes migrate specifically in response to 7alpha,25-dihydroxycholesterol (7alpha,25-OHC) via EBI2 in the MS murine model experimental autoimmune encephalomyelitis.	25-hydroxycholesterol	127-133	G protein-coupled receptor 183	Homo sapiens	139-143
27052460-9	2017	Liquid chromatography and mass spectrometry indicated that the levels of 22-hydroxycholesterol, 25-hydroxycholesterol, and 24,25-epoxycholesterol were higher in the DDC-injured livers of SULT2B1-/- mice than in livers of WT mice.	25-hydroxycholesterol	96-117	sulfotransferase family, cytosolic, 2B, member 1	Mus musculus	187-194
28299341-4	2017	25-Hydroxycholesterol (25-OHC) is produced in cells from cholesterol by the enzyme cholesterol 25-hydroxylase (Ch25h) and is involved in lipid metabolism, inflammatory processes, and cell proliferation.	25-hydroxycholesterol	0-21	cholesterol 25-hydroxylase	Homo sapiens	83-109
28299341-4	2017	25-Hydroxycholesterol (25-OHC) is produced in cells from cholesterol by the enzyme cholesterol 25-hydroxylase (Ch25h) and is involved in lipid metabolism, inflammatory processes, and cell proliferation.	25-hydroxycholesterol	0-21	cholesterol 25-hydroxylase	Homo sapiens	111-116
28299341-4	2017	25-Hydroxycholesterol (25-OHC) is produced in cells from cholesterol by the enzyme cholesterol 25-hydroxylase (Ch25h) and is involved in lipid metabolism, inflammatory processes, and cell proliferation.	25-hydroxycholesterol	23-29	cholesterol 25-hydroxylase	Homo sapiens	83-109
28299341-4	2017	25-Hydroxycholesterol (25-OHC) is produced in cells from cholesterol by the enzyme cholesterol 25-hydroxylase (Ch25h) and is involved in lipid metabolism, inflammatory processes, and cell proliferation.	25-hydroxycholesterol	23-29	cholesterol 25-hydroxylase	Homo sapiens	111-116
27729467-7	2016	Excessive stimulation with the oxysterol 25-hydroxycholesterol disassembles the ORP4L/Galphaq/11/PLCbeta3 complexes, resulting in reduced PLCbeta3 activity, IP3 production, and Ca2+ release, as well as decreased Bcl-XL expression and increased apoptosis.	25-hydroxycholesterol	41-62	guanine nucleotide binding protein, alpha q polypeptide	Mus musculus	86-93
27999160-2	2016	The interferon-stimulated gene cholesterol 25-hydroxylase (CH25H) encodes an enzyme that catalyzes the production of 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	117-138	cholesterol 25-hydroxylase	Homo sapiens	31-57
27999160-2	2016	The interferon-stimulated gene cholesterol 25-hydroxylase (CH25H) encodes an enzyme that catalyzes the production of 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	117-138	cholesterol 25-hydroxylase	Homo sapiens	59-64
27999160-8	2016	Our findings identify a novel role for CH25H in controlling LASV propagation and indicate that manipulation of the expression of CH25H or the administration of 25HC may be a useful anti-LASV therapy.	25-hydroxycholesterol	160-164	cholesterol 25-hydroxylase	Homo sapiens	39-44
27999160-12	2016	Cholesterol 25-hydroxylase (CH25H) is a recently identified interferon-stimulated gene (ISG); it encodes an enzyme that catalyzes the production of 25-hydroxycholesterol (25HC), which inhibits several viruses.	25-hydroxycholesterol	148-169	cholesterol 25-hydroxylase	Homo sapiens	0-26
27999160-12	2016	Cholesterol 25-hydroxylase (CH25H) is a recently identified interferon-stimulated gene (ISG); it encodes an enzyme that catalyzes the production of 25-hydroxycholesterol (25HC), which inhibits several viruses.	25-hydroxycholesterol	148-169	cholesterol 25-hydroxylase	Homo sapiens	28-33
27999160-12	2016	Cholesterol 25-hydroxylase (CH25H) is a recently identified interferon-stimulated gene (ISG); it encodes an enzyme that catalyzes the production of 25-hydroxycholesterol (25HC), which inhibits several viruses.	25-hydroxycholesterol	171-175	cholesterol 25-hydroxylase	Homo sapiens	0-26
27999160-12	2016	Cholesterol 25-hydroxylase (CH25H) is a recently identified interferon-stimulated gene (ISG); it encodes an enzyme that catalyzes the production of 25-hydroxycholesterol (25HC), which inhibits several viruses.	25-hydroxycholesterol	171-175	cholesterol 25-hydroxylase	Homo sapiens	28-33
28989932-5	2017	LXRalpha L414R and R415A lacked binding to T-0901317, but retained binding to 25-Hydroxycholesterol.	25-hydroxycholesterol	78-99	nuclear receptor subfamily 1 group H member 3	Homo sapiens	0-8
27729467-7	2016	Excessive stimulation with the oxysterol 25-hydroxycholesterol disassembles the ORP4L/Galphaq/11/PLCbeta3 complexes, resulting in reduced PLCbeta3 activity, IP3 production, and Ca2+ release, as well as decreased Bcl-XL expression and increased apoptosis.	25-hydroxycholesterol	41-62	phospholipase C, beta 3	Mus musculus	97-105
27729467-7	2016	Excessive stimulation with the oxysterol 25-hydroxycholesterol disassembles the ORP4L/Galphaq/11/PLCbeta3 complexes, resulting in reduced PLCbeta3 activity, IP3 production, and Ca2+ release, as well as decreased Bcl-XL expression and increased apoptosis.	25-hydroxycholesterol	41-62	phospholipase C, beta 3	Mus musculus	138-146
27729467-7	2016	Excessive stimulation with the oxysterol 25-hydroxycholesterol disassembles the ORP4L/Galphaq/11/PLCbeta3 complexes, resulting in reduced PLCbeta3 activity, IP3 production, and Ca2+ release, as well as decreased Bcl-XL expression and increased apoptosis.	25-hydroxycholesterol	41-62	BCL2-like 1	Mus musculus	212-218
27731330-7	2016	Production of 25-hydroxycholesterol is dependent on Lamtor1 and mTORC1.	25-hydroxycholesterol	14-35	late endosomal/lysosomal adaptor, MAPK and MTOR activator 1	Homo sapiens	52-59
27614840-6	2016	Inhibition of SREBP maturation by treatment with 25-hydroxycholesterol (5 microM) for 48h reduced ATRA (1 microM)-induced mRNA concentration of NIS and iodide uptake in MCF-7 cells by approximately 20%.	25-hydroxycholesterol	49-70	solute carrier family 5 member 5	Homo sapiens	144-147
27779191-0	2016	25-hydroxycholesterol contributes to cerebral inflammation of X-linked adrenoleukodystrophy through activation of the NLRP3 inflammasome.	25-hydroxycholesterol	0-21	NLR family pyrin domain containing 3	Homo sapiens	118-123
27779191-5	2016	Furthermore, stereotaxic injection of 25-HC into the corpus callosum of mouse brains induces microglial recruitment, interleukin-1beta production, and oligodendrocyte cell death in an NLRP3 inflammasome-dependent manner.	25-hydroxycholesterol	38-43	interleukin 1 beta	Mus musculus	117-134
27779191-5	2016	Furthermore, stereotaxic injection of 25-HC into the corpus callosum of mouse brains induces microglial recruitment, interleukin-1beta production, and oligodendrocyte cell death in an NLRP3 inflammasome-dependent manner.	25-hydroxycholesterol	38-43	NLR family, pyrin domain containing 3	Mus musculus	184-189
27731330-7	2016	Production of 25-hydroxycholesterol is dependent on Lamtor1 and mTORC1.	25-hydroxycholesterol	14-35	CREB regulated transcription coactivator 1	Mus musculus	64-70
27600825-13	2016	25-OHC reduced the phosphorylation of Akt and eNOS and the association of eNOS and HSP90.	25-hydroxycholesterol	0-6	AKT serine/threonine kinase 1	Rattus norvegicus	38-41
27600825-13	2016	25-OHC reduced the phosphorylation of Akt and eNOS and the association of eNOS and HSP90.	25-hydroxycholesterol	0-6	nitric oxide synthase 3	Rattus norvegicus	46-50
27600825-13	2016	25-OHC reduced the phosphorylation of Akt and eNOS and the association of eNOS and HSP90.	25-hydroxycholesterol	0-6	nitric oxide synthase 3	Rattus norvegicus	74-78
27600825-13	2016	25-OHC reduced the phosphorylation of Akt and eNOS and the association of eNOS and HSP90.	25-hydroxycholesterol	0-6	heat shock protein 90 alpha family class A member 1	Rattus norvegicus	83-88
27600825-14	2016	25-OHC also enhanced endothelial cell apoptosis by decreasing Bcl-2 expression and increasing cleaved caspase-9 and cleaved caspase-3 expressions as well as caspase-3 activity.	25-hydroxycholesterol	0-6	BCL2, apoptosis regulator	Rattus norvegicus	62-67
27600825-14	2016	25-OHC also enhanced endothelial cell apoptosis by decreasing Bcl-2 expression and increasing cleaved caspase-9 and cleaved caspase-3 expressions as well as caspase-3 activity.	25-hydroxycholesterol	0-6	caspase 9	Rattus norvegicus	102-111
27600825-14	2016	25-OHC also enhanced endothelial cell apoptosis by decreasing Bcl-2 expression and increasing cleaved caspase-9 and cleaved caspase-3 expressions as well as caspase-3 activity.	25-hydroxycholesterol	0-6	caspase 3	Rattus norvegicus	124-133
27600825-16	2016	These data demonstrated that 25-OHC could impair endothelial function by uncoupling and inhibiting eNOS activity as well as by inducing endothelial cell apoptosis.	25-hydroxycholesterol	29-35	nitric oxide synthase 3	Rattus norvegicus	99-103
27530118-0	2016	Oxysterol binding protein-related protein 8 mediates the cytotoxicity of 25-hydroxycholesterol.	25-hydroxycholesterol	73-94	oxysterol binding protein like 8	Homo sapiens	0-43
27530118-4	2016	Here, we report that 25-hydroxycholesterol (OHC) induced apoptosis of the hepatoma cell lines, HepG2 and Huh7, via the endoplasmic reticulum (ER) stress response pathway, and ORP8 overexpression resulted in a similar cell response as 25-OHC, indicating a putative functional relationship between oxysterol cytotoxicity and ORP8.	25-hydroxycholesterol	21-42	MIR7-3 host gene	Homo sapiens	105-109
27530118-4	2016	Here, we report that 25-hydroxycholesterol (OHC) induced apoptosis of the hepatoma cell lines, HepG2 and Huh7, via the endoplasmic reticulum (ER) stress response pathway, and ORP8 overexpression resulted in a similar cell response as 25-OHC, indicating a putative functional relationship between oxysterol cytotoxicity and ORP8.	25-hydroxycholesterol	21-42	oxysterol binding protein like 8	Homo sapiens	175-179
27530118-4	2016	Here, we report that 25-hydroxycholesterol (OHC) induced apoptosis of the hepatoma cell lines, HepG2 and Huh7, via the endoplasmic reticulum (ER) stress response pathway, and ORP8 overexpression resulted in a similar cell response as 25-OHC, indicating a putative functional relationship between oxysterol cytotoxicity and ORP8.	25-hydroxycholesterol	21-42	oxysterol binding protein like 8	Homo sapiens	323-327
27530118-8	2016	Taken together, the present study suggests that ORP8 may mediate the cytotoxicity of 25-OHC.	25-hydroxycholesterol	85-91	oxysterol binding protein like 8	Homo sapiens	48-52
26657973-6	2015	LC extraction by 25-hydroxycholesterol increased APAP-mediated mitophagy and protected ASMase(-/-) mice and hepatocytes against APAP hepatotoxicity, effects that were reversed by chloroquine to disrupt autophagy.	25-hydroxycholesterol	17-38	sphingomyelin phosphodiesterase 1, acid lysosomal	Mus musculus	87-93
27600825-14	2016	25-OHC also enhanced endothelial cell apoptosis by decreasing Bcl-2 expression and increasing cleaved caspase-9 and cleaved caspase-3 expressions as well as caspase-3 activity.	25-hydroxycholesterol	0-6	caspase 3	Rattus norvegicus	157-166
27109381-8	2016	This activation was dependent on pannexin-1 with 25-hydroxycholesterol whereas P2X7 receptor signaling pathway was pannexin-1-independent for 7-ketocholesterol.	25-hydroxycholesterol	49-70	pannexin 1	Homo sapiens	33-43
26601944-2	2016	25-Hydroxycholesterol (25OH) competitively inhibits this exchange reaction in vitro and causes the constitutive localization of OSBP at the ER/Golgi interface and PI-4P-dependent recruitment of ceramide transfer protein (CERT) for sphingomyelin synthesis.	25-hydroxycholesterol	0-21	ceramide transfer protein	Cricetulus griseus	194-219
26601944-2	2016	25-Hydroxycholesterol (25OH) competitively inhibits this exchange reaction in vitro and causes the constitutive localization of OSBP at the ER/Golgi interface and PI-4P-dependent recruitment of ceramide transfer protein (CERT) for sphingomyelin synthesis.	25-hydroxycholesterol	0-21	ceramide transfer protein	Cricetulus griseus	221-225
26601944-2	2016	25-Hydroxycholesterol (25OH) competitively inhibits this exchange reaction in vitro and causes the constitutive localization of OSBP at the ER/Golgi interface and PI-4P-dependent recruitment of ceramide transfer protein (CERT) for sphingomyelin synthesis.	25-hydroxycholesterol	23-27	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	128-132
26601944-2	2016	25-Hydroxycholesterol (25OH) competitively inhibits this exchange reaction in vitro and causes the constitutive localization of OSBP at the ER/Golgi interface and PI-4P-dependent recruitment of ceramide transfer protein (CERT) for sphingomyelin synthesis.	25-hydroxycholesterol	23-27	ceramide transfer protein	Cricetulus griseus	194-219
26601944-2	2016	25-Hydroxycholesterol (25OH) competitively inhibits this exchange reaction in vitro and causes the constitutive localization of OSBP at the ER/Golgi interface and PI-4P-dependent recruitment of ceramide transfer protein (CERT) for sphingomyelin synthesis.	25-hydroxycholesterol	23-27	ceramide transfer protein	Cricetulus griseus	221-225
26601944-4	2016	Treatment of fibroblasts or Chinese hamster ovary (CHO) cells with 25OH caused a 50-70% reduction in Golgi-associated immunoreactive PI-4P that correlated with Golgi localization of OSBP.	25-hydroxycholesterol	67-71	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	182-186
26601944-5	2016	In contrast, 25OH caused an OSBP-dependent enrichment in Golgi PI-4P that was detected with a pleckstrin homology domain probe.	25-hydroxycholesterol	13-17	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	28-32
26601944-7	2016	The PI-4P and sterol binding activities of OSBP were both required for 25OH activation of sphingomyelin synthesis, suggesting that 25OH must be exchanged for PI-4P to be concentrated at contact sites.	25-hydroxycholesterol	71-75	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	43-47
26601944-7	2016	The PI-4P and sterol binding activities of OSBP were both required for 25OH activation of sphingomyelin synthesis, suggesting that 25OH must be exchanged for PI-4P to be concentrated at contact sites.	25-hydroxycholesterol	131-135	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	43-47
26601944-8	2016	We propose a model wherein 25OH activation of OSBP promotes the binding and retention of PI-4P at ER-Golgi contact sites.	25-hydroxycholesterol	27-31	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	46-50
27354064-7	2016	Treatment of cells with 25-hydroxycholesterol or statins, which respectively inhibit or activate SREBP, further supports SREBP-mediated regulation of IDH1 and, in cells with oncogenic IDH1, carbon flux into 2-HG.	25-hydroxycholesterol	24-45	CCHC-type zinc finger nucleic acid binding protein	Homo sapiens	97-102
27354064-7	2016	Treatment of cells with 25-hydroxycholesterol or statins, which respectively inhibit or activate SREBP, further supports SREBP-mediated regulation of IDH1 and, in cells with oncogenic IDH1, carbon flux into 2-HG.	25-hydroxycholesterol	24-45	CCHC-type zinc finger nucleic acid binding protein	Homo sapiens	121-126
27354064-7	2016	Treatment of cells with 25-hydroxycholesterol or statins, which respectively inhibit or activate SREBP, further supports SREBP-mediated regulation of IDH1 and, in cells with oncogenic IDH1, carbon flux into 2-HG.	25-hydroxycholesterol	24-45	isocitrate dehydrogenase (NADP(+)) 1	Homo sapiens	150-154
27354064-7	2016	Treatment of cells with 25-hydroxycholesterol or statins, which respectively inhibit or activate SREBP, further supports SREBP-mediated regulation of IDH1 and, in cells with oncogenic IDH1, carbon flux into 2-HG.	25-hydroxycholesterol	24-45	isocitrate dehydrogenase (NADP(+)) 1	Homo sapiens	184-188
26601944-2	2016	25-Hydroxycholesterol (25OH) competitively inhibits this exchange reaction in vitro and causes the constitutive localization of OSBP at the ER/Golgi interface and PI-4P-dependent recruitment of ceramide transfer protein (CERT) for sphingomyelin synthesis.	25-hydroxycholesterol	0-21	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	128-132
26230055-2	2015	Addition of cholesterol 25-hydroperoxide to the enzymes CYP27A1 and CYP11A1 induced well-defined spectral changes while generating 25-hydroxycholesterol as the major product.	25-hydroxycholesterol	131-152	cytochrome P450 family 27 subfamily A member 1	Homo sapiens	56-63
26479438-4	2015	We identified miR-130b and miR-185 as hepatic microRNAs (miRNAs) whose expression is stimulated by 25-hydroxycholesterol (25-HC), an antiviral oxysterol secreted by interferon-stimulated macrophages and dendritic cells, during hepatitis C virus (HCV) infection.	25-hydroxycholesterol	99-120	microRNA 185	Homo sapiens	27-34
26577244-3	2015	CH25H catalyzes hydroxylation of cholesterol and produces 25-hydroxycholesterol (25-OHC).	25-hydroxycholesterol	58-79	cholesterol 25-hydroxylase	Homo sapiens	0-5
26577244-3	2015	CH25H catalyzes hydroxylation of cholesterol and produces 25-hydroxycholesterol (25-OHC).	25-hydroxycholesterol	81-87	cholesterol 25-hydroxylase	Homo sapiens	0-5
26310456-0	2015	Transcriptional suppression of CTP:phosphoethanolamine cytidylyltransferase by 25-hydroxycholesterol is mediated by nuclear factor-Y and Yin Yang 1.	25-hydroxycholesterol	79-100	phosphate cytidylyltransferase 2, ethanolamine	Homo sapiens	31-75
26310456-0	2015	Transcriptional suppression of CTP:phosphoethanolamine cytidylyltransferase by 25-hydroxycholesterol is mediated by nuclear factor-Y and Yin Yang 1.	25-hydroxycholesterol	79-100	YY1 transcription factor	Homo sapiens	137-147
26438360-3	2015	Here, we show that the Galphai protein-coupled receptor (GPCR) EBI2 is expressed in mouse monocyte/OC precursors (OCPs) and its oxysterol ligand 7alpha,25-dihydroxycholesterol (7alpha,25-OHC) is secreted abundantly by OBs.	25-hydroxycholesterol	184-190	G protein-coupled receptor 183	Mus musculus	63-67
26122972-4	2015	7-Ketocholesterol and 25-hydroxycholesterol decreased the levels of Bid and Bcl-2, increased the levels of Bax and p53, and induced loss of the mitochondrial transmembrane potential, release of cytochrome c and activation of caspases (-8, -9 and -3).	25-hydroxycholesterol	22-43	BH3 interacting domain death agonist	Rattus norvegicus	68-71
26122972-4	2015	7-Ketocholesterol and 25-hydroxycholesterol decreased the levels of Bid and Bcl-2, increased the levels of Bax and p53, and induced loss of the mitochondrial transmembrane potential, release of cytochrome c and activation of caspases (-8, -9 and -3).	25-hydroxycholesterol	22-43	BCL2, apoptosis regulator	Rattus norvegicus	76-81
26122972-4	2015	7-Ketocholesterol and 25-hydroxycholesterol decreased the levels of Bid and Bcl-2, increased the levels of Bax and p53, and induced loss of the mitochondrial transmembrane potential, release of cytochrome c and activation of caspases (-8, -9 and -3).	25-hydroxycholesterol	22-43	BCL2 associated X, apoptosis regulator	Rattus norvegicus	107-110
26122972-4	2015	7-Ketocholesterol and 25-hydroxycholesterol decreased the levels of Bid and Bcl-2, increased the levels of Bax and p53, and induced loss of the mitochondrial transmembrane potential, release of cytochrome c and activation of caspases (-8, -9 and -3).	25-hydroxycholesterol	22-43	Wistar clone pR53P1 p53 pseudogene	Rattus norvegicus	115-118
26230055-2	2015	Addition of cholesterol 25-hydroperoxide to the enzymes CYP27A1 and CYP11A1 induced well-defined spectral changes while generating 25-hydroxycholesterol as the major product.	25-hydroxycholesterol	131-152	cytochrome P450 family 11 subfamily A member 1	Homo sapiens	68-75
26230055-6	2015	CYP27A1 was shown to mediate the reduction of cholesterol 25-hydroperoxide to 25-hydroxycholesterol, a role of potential significance for cholesterol-rich tissues with high oxidative stress.	25-hydroxycholesterol	78-99	cytochrome P450 family 27 subfamily A member 1	Homo sapiens	0-7
26230055-7	2015	CYP27A1 may participate in the removal of harmful autoxidation products in these tissues, while providing a complementary source of 25-hydroxycholesterol, a modulator of immune cell function and mediator of viral cell entry.	25-hydroxycholesterol	132-153	cytochrome P450 family 27 subfamily A member 1	Homo sapiens	0-7
25999047-3	2015	Recent studies have reported that cholesterol 25-hydroxylase (CH25H) is expressed as an interferon-stimulated gene and mediates antiviral activities against different enveloped viruses through the production of 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	211-232	cholesterol 25-hydroxylase	Homo sapiens	62-67
26344074-2	2015	We recently reported that 25-hydroxycholesterol, a representative LXR-activating oxysterol, suppresses IL-6 production in mouse mast cells (MCs) following its engagement of the high-affinity IgE receptor (FcepsilonRI).	25-hydroxycholesterol	26-47	nuclear receptor subfamily 1, group H, member 2	Mus musculus	66-69
26344074-2	2015	We recently reported that 25-hydroxycholesterol, a representative LXR-activating oxysterol, suppresses IL-6 production in mouse mast cells (MCs) following its engagement of the high-affinity IgE receptor (FcepsilonRI).	25-hydroxycholesterol	26-47	interleukin 6	Mus musculus	103-107
26344074-2	2015	We recently reported that 25-hydroxycholesterol, a representative LXR-activating oxysterol, suppresses IL-6 production in mouse mast cells (MCs) following its engagement of the high-affinity IgE receptor (FcepsilonRI).	25-hydroxycholesterol	26-47	Fc receptor, IgE, high affinity I, alpha polypeptide	Mus musculus	205-216
26122972-4	2015	7-Ketocholesterol and 25-hydroxycholesterol decreased the levels of Bid and Bcl-2, increased the levels of Bax and p53, and induced loss of the mitochondrial transmembrane potential, release of cytochrome c and activation of caspases (-8, -9 and -3).	25-hydroxycholesterol	22-43	caspase 8	Rattus norvegicus	225-248
25999047-3	2015	Recent studies have reported that cholesterol 25-hydroxylase (CH25H) is expressed as an interferon-stimulated gene and mediates antiviral activities against different enveloped viruses through the production of 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	234-238	cholesterol 25-hydroxylase	Homo sapiens	34-60
25999047-3	2015	Recent studies have reported that cholesterol 25-hydroxylase (CH25H) is expressed as an interferon-stimulated gene and mediates antiviral activities against different enveloped viruses through the production of 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	234-238	cholesterol 25-hydroxylase	Homo sapiens	62-67
25845326-3	2015	7-Ketocholesterol and 25-hydroxycholesterol induced a decrease in the levels of BH3 interacting-domain death agonist (Bid) and B cell lymphoma 2 (Bcl-2), increase in the levels of Bcl-2-associated X protein (Bax) and p53, loss of the mitochondrial transmembrane potential, cytochrome c release, activation of caspases, and cleavage of poly(ADP-ribose) polymerase 1 (PARP-1).	25-hydroxycholesterol	22-43	BH3 interacting domain death agonist	Rattus norvegicus	118-121
25999047-3	2015	Recent studies have reported that cholesterol 25-hydroxylase (CH25H) is expressed as an interferon-stimulated gene and mediates antiviral activities against different enveloped viruses through the production of 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	211-232	cholesterol 25-hydroxylase	Homo sapiens	34-60
25845326-3	2015	7-Ketocholesterol and 25-hydroxycholesterol induced a decrease in the levels of BH3 interacting-domain death agonist (Bid) and B cell lymphoma 2 (Bcl-2), increase in the levels of Bcl-2-associated X protein (Bax) and p53, loss of the mitochondrial transmembrane potential, cytochrome c release, activation of caspases, and cleavage of poly(ADP-ribose) polymerase 1 (PARP-1).	25-hydroxycholesterol	22-43	BCL2, apoptosis regulator	Rattus norvegicus	127-144
25845326-3	2015	7-Ketocholesterol and 25-hydroxycholesterol induced a decrease in the levels of BH3 interacting-domain death agonist (Bid) and B cell lymphoma 2 (Bcl-2), increase in the levels of Bcl-2-associated X protein (Bax) and p53, loss of the mitochondrial transmembrane potential, cytochrome c release, activation of caspases, and cleavage of poly(ADP-ribose) polymerase 1 (PARP-1).	25-hydroxycholesterol	22-43	BCL2, apoptosis regulator	Rattus norvegicus	146-151
25845326-3	2015	7-Ketocholesterol and 25-hydroxycholesterol induced a decrease in the levels of BH3 interacting-domain death agonist (Bid) and B cell lymphoma 2 (Bcl-2), increase in the levels of Bcl-2-associated X protein (Bax) and p53, loss of the mitochondrial transmembrane potential, cytochrome c release, activation of caspases, and cleavage of poly(ADP-ribose) polymerase 1 (PARP-1).	25-hydroxycholesterol	22-43	BCL2 associated X, apoptosis regulator	Rattus norvegicus	180-206
25845326-3	2015	7-Ketocholesterol and 25-hydroxycholesterol induced a decrease in the levels of BH3 interacting-domain death agonist (Bid) and B cell lymphoma 2 (Bcl-2), increase in the levels of Bcl-2-associated X protein (Bax) and p53, loss of the mitochondrial transmembrane potential, cytochrome c release, activation of caspases, and cleavage of poly(ADP-ribose) polymerase 1 (PARP-1).	25-hydroxycholesterol	22-43	BCL2 associated X, apoptosis regulator	Rattus norvegicus	208-211
25845326-3	2015	7-Ketocholesterol and 25-hydroxycholesterol induced a decrease in the levels of BH3 interacting-domain death agonist (Bid) and B cell lymphoma 2 (Bcl-2), increase in the levels of Bcl-2-associated X protein (Bax) and p53, loss of the mitochondrial transmembrane potential, cytochrome c release, activation of caspases, and cleavage of poly(ADP-ribose) polymerase 1 (PARP-1).	25-hydroxycholesterol	22-43	Wistar clone pR53P1 p53 pseudogene	Rattus norvegicus	217-220
25845326-3	2015	7-Ketocholesterol and 25-hydroxycholesterol induced a decrease in the levels of BH3 interacting-domain death agonist (Bid) and B cell lymphoma 2 (Bcl-2), increase in the levels of Bcl-2-associated X protein (Bax) and p53, loss of the mitochondrial transmembrane potential, cytochrome c release, activation of caspases, and cleavage of poly(ADP-ribose) polymerase 1 (PARP-1).	25-hydroxycholesterol	22-43	caspase 8	Rattus norvegicus	309-317
25845326-3	2015	7-Ketocholesterol and 25-hydroxycholesterol induced a decrease in the levels of BH3 interacting-domain death agonist (Bid) and B cell lymphoma 2 (Bcl-2), increase in the levels of Bcl-2-associated X protein (Bax) and p53, loss of the mitochondrial transmembrane potential, cytochrome c release, activation of caspases, and cleavage of poly(ADP-ribose) polymerase 1 (PARP-1).	25-hydroxycholesterol	22-43	poly (ADP-ribose) polymerase 1	Rattus norvegicus	335-364
25845326-3	2015	7-Ketocholesterol and 25-hydroxycholesterol induced a decrease in the levels of BH3 interacting-domain death agonist (Bid) and B cell lymphoma 2 (Bcl-2), increase in the levels of Bcl-2-associated X protein (Bax) and p53, loss of the mitochondrial transmembrane potential, cytochrome c release, activation of caspases, and cleavage of poly(ADP-ribose) polymerase 1 (PARP-1).	25-hydroxycholesterol	22-43	poly (ADP-ribose) polymerase 1	Rattus norvegicus	366-372
26023184-8	2015	Conversely, activation of LXRs by either 25-hydroxycholesterol or synthetic TO901317 stimulates myelin gene expression at the promoter, mRNA, and protein levels, directly implicating LXRalpha/beta in the transcriptional control of myelin gene expression.	25-hydroxycholesterol	41-62	nuclear receptor subfamily 1, group H, member 3	Mus musculus	183-196
26160948-8	2015	A homology-based structural model of human Insig-2, together with biochemical characterizations, suggest that the central cavity of Insig-2 accommodates 25-hydroxycholesterol, whereas TM3 and TM4 engage in Scap binding.	25-hydroxycholesterol	153-174	insulin induced gene 2	Homo sapiens	43-50
26160948-8	2015	A homology-based structural model of human Insig-2, together with biochemical characterizations, suggest that the central cavity of Insig-2 accommodates 25-hydroxycholesterol, whereas TM3 and TM4 engage in Scap binding.	25-hydroxycholesterol	153-174	insulin induced gene 2	Homo sapiens	132-139
25759117-0	2015	Rapid proteasomal elimination of 3-hydroxy-3-methylglutaryl-CoA reductase by interferon-gamma in primary macrophages requires endogenous 25-hydroxycholesterol synthesis.	25-hydroxycholesterol	137-158	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	33-73
25759117-0	2015	Rapid proteasomal elimination of 3-hydroxy-3-methylglutaryl-CoA reductase by interferon-gamma in primary macrophages requires endogenous 25-hydroxycholesterol synthesis.	25-hydroxycholesterol	137-158	interferon gamma	Homo sapiens	77-93
25456971-5	2015	The enzyme cholesterol 25 hydroxylase (Ch25h) is the rate limiting step to synthesize the oxysterol 7alpha,25-dihydroxycholesterol (7alpha,25-OHC) from cholesterol.	25-hydroxycholesterol	139-145	cholesterol 25-hydroxylase	Homo sapiens	39-44
25852561-4	2015	A few subsequently published studies further elucidated how 7alpha, 25-OHC bound to EBI2, and how a gradient of 7alpha, 25-OHC could be generated in vivo and regulated migration, activation, and functions of B cells, T cells, dendritic cells (DCs), monocytes/macrophages, and astrocytes.	25-hydroxycholesterol	68-74	G protein-coupled receptor 183	Homo sapiens	84-88
25592323-4	2015	7-Ketocholesterol and 25-hydroxycholesterol induced a decrease in Bid, Bcl-2, Bcl-xL and survivin protein levels, increase in Bax levels, loss of the mitochondrial transmembrane potential, cytochrome c release, activation of caspases (-8, -9 and -3), cleavage of PARP-1 and an increase in the tumor suppressor p53 levels.	25-hydroxycholesterol	22-43	BH3 interacting domain death agonist	Rattus norvegicus	66-69
25592323-4	2015	7-Ketocholesterol and 25-hydroxycholesterol induced a decrease in Bid, Bcl-2, Bcl-xL and survivin protein levels, increase in Bax levels, loss of the mitochondrial transmembrane potential, cytochrome c release, activation of caspases (-8, -9 and -3), cleavage of PARP-1 and an increase in the tumor suppressor p53 levels.	25-hydroxycholesterol	22-43	BCL2, apoptosis regulator	Rattus norvegicus	71-76
25592323-4	2015	7-Ketocholesterol and 25-hydroxycholesterol induced a decrease in Bid, Bcl-2, Bcl-xL and survivin protein levels, increase in Bax levels, loss of the mitochondrial transmembrane potential, cytochrome c release, activation of caspases (-8, -9 and -3), cleavage of PARP-1 and an increase in the tumor suppressor p53 levels.	25-hydroxycholesterol	22-43	Bcl2-like 1	Rattus norvegicus	78-84
25592323-4	2015	7-Ketocholesterol and 25-hydroxycholesterol induced a decrease in Bid, Bcl-2, Bcl-xL and survivin protein levels, increase in Bax levels, loss of the mitochondrial transmembrane potential, cytochrome c release, activation of caspases (-8, -9 and -3), cleavage of PARP-1 and an increase in the tumor suppressor p53 levels.	25-hydroxycholesterol	22-43	BCL2 associated X, apoptosis regulator	Rattus norvegicus	126-129
25592323-4	2015	7-Ketocholesterol and 25-hydroxycholesterol induced a decrease in Bid, Bcl-2, Bcl-xL and survivin protein levels, increase in Bax levels, loss of the mitochondrial transmembrane potential, cytochrome c release, activation of caspases (-8, -9 and -3), cleavage of PARP-1 and an increase in the tumor suppressor p53 levels.	25-hydroxycholesterol	22-43	poly (ADP-ribose) polymerase 1	Rattus norvegicus	263-269
25592323-4	2015	7-Ketocholesterol and 25-hydroxycholesterol induced a decrease in Bid, Bcl-2, Bcl-xL and survivin protein levels, increase in Bax levels, loss of the mitochondrial transmembrane potential, cytochrome c release, activation of caspases (-8, -9 and -3), cleavage of PARP-1 and an increase in the tumor suppressor p53 levels.	25-hydroxycholesterol	22-43	Wistar clone pR53P1 p53 pseudogene	Rattus norvegicus	310-313
25456971-7	2015	Mechanistically, we show a critical involvement for oxysterols in recruiting leukocytes into inflamed tissues and propose that 7alpha,25-OHC preferentially promotes the migration of activated CD44(+)CD4(+) T cells by binding the G protein-coupled receptor called Epstein-Barr virus induced gene 2 (EBI2).	25-hydroxycholesterol	134-140	CD4 antigen	Mus musculus	192-195
25018046-9	2014	In addition, in vitro experiments suggested that exogenous cholesterol, 25-hydroxycholesterol and mevinolin (a highly potent competitive inhibitor of HMG-CoA) treatment could significantly alter TypeII VLDLR gene expression in granulosa cells from both pre-hierarchical and pre-ovulatory follicles.	25-hydroxycholesterol	72-93	very low density lipoprotein receptor	Homo sapiens	202-207
25463072-7	2014	RESULTS: In separate SMC and monocyte cultures (monocultures) 25-hydroxycholesterol only poorly activated IL-1, IL-6 and MCP-1 production, whereas LPS stimulated much higher cytokine levels than unstimulated cultures.	25-hydroxycholesterol	62-83	interleukin 1 beta	Homo sapiens	106-110
25463072-7	2014	RESULTS: In separate SMC and monocyte cultures (monocultures) 25-hydroxycholesterol only poorly activated IL-1, IL-6 and MCP-1 production, whereas LPS stimulated much higher cytokine levels than unstimulated cultures.	25-hydroxycholesterol	62-83	interleukin 6	Homo sapiens	112-116
25463072-7	2014	RESULTS: In separate SMC and monocyte cultures (monocultures) 25-hydroxycholesterol only poorly activated IL-1, IL-6 and MCP-1 production, whereas LPS stimulated much higher cytokine levels than unstimulated cultures.	25-hydroxycholesterol	62-83	C-C motif chemokine ligand 2	Homo sapiens	121-126
25467815-1	2014	Cholesterol 25-hydroxylase (CH25H) as an interferon-stimulated gene (ISG) has recently been shown to exert broad antiviral activity through the production of 25-hydroxycholesterol (25HC), which is believed to inhibit the virus-cell membrane fusion during viral entry.	25-hydroxycholesterol	158-179	cholesterol 25-hydroxylase	Homo sapiens	0-26
25467815-1	2014	Cholesterol 25-hydroxylase (CH25H) as an interferon-stimulated gene (ISG) has recently been shown to exert broad antiviral activity through the production of 25-hydroxycholesterol (25HC), which is believed to inhibit the virus-cell membrane fusion during viral entry.	25-hydroxycholesterol	158-179	cholesterol 25-hydroxylase	Homo sapiens	28-33
25467815-1	2014	Cholesterol 25-hydroxylase (CH25H) as an interferon-stimulated gene (ISG) has recently been shown to exert broad antiviral activity through the production of 25-hydroxycholesterol (25HC), which is believed to inhibit the virus-cell membrane fusion during viral entry.	25-hydroxycholesterol	181-185	cholesterol 25-hydroxylase	Homo sapiens	0-26
25467815-1	2014	Cholesterol 25-hydroxylase (CH25H) as an interferon-stimulated gene (ISG) has recently been shown to exert broad antiviral activity through the production of 25-hydroxycholesterol (25HC), which is believed to inhibit the virus-cell membrane fusion during viral entry.	25-hydroxycholesterol	181-185	cholesterol 25-hydroxylase	Homo sapiens	28-33
24891333-0	2014	Absence of Nceh1 augments 25-hydroxycholesterol-induced ER stress and apoptosis in macrophages.	25-hydroxycholesterol	26-47	neutral cholesterol ester hydrolase 1	Mus musculus	11-16
24719409-13	2014	CH25H and its product, 25-hydroxycholesterol, restrict replication of diverse virus families.	25-hydroxycholesterol	23-44	cholesterol 25-hydroxylase	Homo sapiens	0-5
24833118-0	2014	3beta,5alpha,6beta-Cholestanetriol and 25-hydroxycholesterol accumulate in ATP-binding cassette transporter G1 (ABCG1)-deficiency.	25-hydroxycholesterol	39-60	ATP binding cassette subfamily G member 1	Mus musculus	75-110
25104388-1	2014	25-Hydroxycholesterol suppresses interleukin-1-driven inflammation downstream of type I interferon.	25-hydroxycholesterol	0-21	interleukin 1 complex	Mus musculus	33-46
24480442-1	2014	Oxysterols such as 7 alpha, 25-dihydroxycholesterol (7alpha,25-OHC) are natural ligands for the Epstein-Barr virus (EBV)-induced gene 2 (EBI2, aka GPR183), a G protein-coupled receptor (GPCR) highly expressed in immune cells and required for adaptive immune responses.	25-hydroxycholesterol	60-66	G protein-coupled receptor 183	Homo sapiens	137-141
23500534-5	2013	We observed a differential effect of 25-OH toward myelin genes (MPZ and PMP22) expression: 25-OH inhibits MPZ and PMP22 in Schwann cell line but not in oligodendrocyte cell line.	25-hydroxycholesterol	37-42	myelin protein zero	Mus musculus	64-67
24321096-7	2014	In chromatin immunoprecipitation assays, specific binding of FXR to the IR-1-containing region of the FGF19 gene (+3214 to +3404) was increased in LS174T cells by treatment with cholesterol and 25-hydroxycholesterol.	25-hydroxycholesterol	194-215	nuclear receptor subfamily 1 group H member 4	Homo sapiens	61-64
24321096-7	2014	In chromatin immunoprecipitation assays, specific binding of FXR to the IR-1-containing region of the FGF19 gene (+3214 to +3404) was increased in LS174T cells by treatment with cholesterol and 25-hydroxycholesterol.	25-hydroxycholesterol	194-215	fibroblast growth factor 19	Homo sapiens	102-107
24625548-3	2014	Thyroid epithelial cells treated with 25-hydroxycholesterol, which is known to inhibit SREBP activation, had about 50% decreased mRNA levels of TPO.	25-hydroxycholesterol	38-59	thyroid peroxidase	Rattus norvegicus	144-147
25289390-11	2014	Additionally, in primary rat hepatocytes treated with cholesterol and 25-hydroxycholesterol or simvastatin, ACMSD gene and protein expression was subjected to sterol-dependent regulation.	25-hydroxycholesterol	70-91	aminocarboxymuconate semialdehyde decarboxylase	Rattus norvegicus	108-113
23541792-8	2013	Experiments revealed that 25-OHC and lipid extracts isolated from GM133-conditioned medium (containing 7-fold higher 25-OHC concentrations than U87MG medium) induce chemotactic migration of THP-1 cells.	25-hydroxycholesterol	26-32	GLI family zinc finger 2	Homo sapiens	190-195
23541792-8	2013	Experiments revealed that 25-OHC and lipid extracts isolated from GM133-conditioned medium (containing 7-fold higher 25-OHC concentrations than U87MG medium) induce chemotactic migration of THP-1 cells.	25-hydroxycholesterol	117-123	GLI family zinc finger 2	Homo sapiens	190-195
23541792-10	2013	In response to exogenously added 25-OHC, THP-1 cells reorganized intermediate filament-associated vimentin to more cortical and polarized structures.	25-hydroxycholesterol	33-39	GLI family zinc finger 2	Homo sapiens	41-46
23541792-10	2013	In response to exogenously added 25-OHC, THP-1 cells reorganized intermediate filament-associated vimentin to more cortical and polarized structures.	25-hydroxycholesterol	33-39	vimentin	Homo sapiens	98-106
23500534-5	2013	We observed a differential effect of 25-OH toward myelin genes (MPZ and PMP22) expression: 25-OH inhibits MPZ and PMP22 in Schwann cell line but not in oligodendrocyte cell line.	25-hydroxycholesterol	37-42	peripheral myelin protein 22	Mus musculus	72-77
23500534-5	2013	We observed a differential effect of 25-OH toward myelin genes (MPZ and PMP22) expression: 25-OH inhibits MPZ and PMP22 in Schwann cell line but not in oligodendrocyte cell line.	25-hydroxycholesterol	91-96	myelin protein zero	Mus musculus	64-67
23500534-5	2013	We observed a differential effect of 25-OH toward myelin genes (MPZ and PMP22) expression: 25-OH inhibits MPZ and PMP22 in Schwann cell line but not in oligodendrocyte cell line.	25-hydroxycholesterol	91-96	peripheral myelin protein 22	Mus musculus	72-77
23500534-5	2013	We observed a differential effect of 25-OH toward myelin genes (MPZ and PMP22) expression: 25-OH inhibits MPZ and PMP22 in Schwann cell line but not in oligodendrocyte cell line.	25-hydroxycholesterol	91-96	myelin protein zero	Mus musculus	106-109
23500534-5	2013	We observed a differential effect of 25-OH toward myelin genes (MPZ and PMP22) expression: 25-OH inhibits MPZ and PMP22 in Schwann cell line but not in oligodendrocyte cell line.	25-hydroxycholesterol	91-96	peripheral myelin protein 22	Mus musculus	114-119
23500534-9	2013	Furthermore, we show by immunofluorescent labeling that beta-catenin is re-localized on the level of the Golgi apparatus of Schwann cells after incubation with 25-OH.	25-hydroxycholesterol	160-165	catenin (cadherin associated protein), beta 1	Mus musculus	56-68
22999953-2	2012	7alpha,25-OHC is synthesized from cholesterol by the stepwise actions of two enzymes, CH25H and CYP7B1, and is metabolized to a 3-oxo derivative by HSD3B7.	25-hydroxycholesterol	7-13	cholesterol 25-hydroxylase	Homo sapiens	86-91
23502855-3	2013	EBI2 and its main ligand, 7alpha,25-OHC, were required for the generation of the splenic CD4(+) DC subset and the localization of DCs in bridging channels.	25-hydroxycholesterol	33-39	G protein-coupled receptor 183	Homo sapiens	0-4
23502855-3	2013	EBI2 and its main ligand, 7alpha,25-OHC, were required for the generation of the splenic CD4(+) DC subset and the localization of DCs in bridging channels.	25-hydroxycholesterol	33-39	CD4 molecule	Homo sapiens	89-92
22732193-4	2013	A number of different cytochrome P450 enzymes such as CYP27A1 and CYP3A4 have been reported to catalyze the conversion of cholesterol to 25-hydroxycholesterol in vitro, but the importance of these reactions in vivo remains unclear.	25-hydroxycholesterol	137-158	cytochrome P450, family 27, subfamily a, polypeptide 1	Mus musculus	54-61
22732193-5	2013	The dioxygenase enzyme cholesterol 25-hydroxylase has been shown to generate 25-hydroxycholesterol, but in cholesterol 25-hydroxylase knockout mice there are still significant levels of 25-hydroxycholesterol in several tissues.	25-hydroxycholesterol	77-98	cholesterol 25-hydroxylase	Mus musculus	23-49
22732193-5	2013	The dioxygenase enzyme cholesterol 25-hydroxylase has been shown to generate 25-hydroxycholesterol, but in cholesterol 25-hydroxylase knockout mice there are still significant levels of 25-hydroxycholesterol in several tissues.	25-hydroxycholesterol	186-207	cholesterol 25-hydroxylase	Mus musculus	23-49
22732193-5	2013	The dioxygenase enzyme cholesterol 25-hydroxylase has been shown to generate 25-hydroxycholesterol, but in cholesterol 25-hydroxylase knockout mice there are still significant levels of 25-hydroxycholesterol in several tissues.	25-hydroxycholesterol	186-207	cholesterol 25-hydroxylase	Mus musculus	107-133
23197320-6	2013	Giving cells lipoproteins or 25-hydroxycholesterol downregulated Ldlr but not Scarb1; Scarb1 was ultimately downregulated by excessive sterol accumulation under 25-hydroxycholesterol and aminoglutethimide (inhibitor of steroidogenesis) cotreatment.	25-hydroxycholesterol	29-50	low density lipoprotein receptor	Rattus norvegicus	65-69
23197320-6	2013	Giving cells lipoproteins or 25-hydroxycholesterol downregulated Ldlr but not Scarb1; Scarb1 was ultimately downregulated by excessive sterol accumulation under 25-hydroxycholesterol and aminoglutethimide (inhibitor of steroidogenesis) cotreatment.	25-hydroxycholesterol	29-50	scavenger receptor class B, member 1	Rattus norvegicus	86-92
23266613-0	2013	p38MAPK and Rho-dependent kinase are involved in anoikis induced by anicequol or 25-hydroxycholesterol in DLD-1 colon cancer cells.	25-hydroxycholesterol	81-102	mitogen-activated protein kinase 14	Homo sapiens	0-7
23273844-3	2013	CH25H converts cholesterol to a soluble antiviral factor, 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	81-85	cholesterol 25-hydroxylase	Mus musculus	0-5
23485764-2	2013	25-hydroxycholesterol (25-HC) is enzymatically produced by cholesterol 25-hydorxylase (CH25H) in macrophages and is reported to be involved in the formation of arteriosclerosis.	25-hydroxycholesterol	0-21	cholesterol 25-hydroxylase	Homo sapiens	59-85
23485764-2	2013	25-hydroxycholesterol (25-HC) is enzymatically produced by cholesterol 25-hydorxylase (CH25H) in macrophages and is reported to be involved in the formation of arteriosclerosis.	25-hydroxycholesterol	0-21	cholesterol 25-hydroxylase	Homo sapiens	87-92
23485764-2	2013	25-hydroxycholesterol (25-HC) is enzymatically produced by cholesterol 25-hydorxylase (CH25H) in macrophages and is reported to be involved in the formation of arteriosclerosis.	25-hydroxycholesterol	23-28	cholesterol 25-hydroxylase	Homo sapiens	59-85
23485764-2	2013	25-hydroxycholesterol (25-HC) is enzymatically produced by cholesterol 25-hydorxylase (CH25H) in macrophages and is reported to be involved in the formation of arteriosclerosis.	25-hydroxycholesterol	23-28	cholesterol 25-hydroxylase	Homo sapiens	87-92
23485764-7	2013	25-HC also significantly enhanced the release and activation of matrix metallaoproteinase (MMP)-2 (P&lt;0.001) and MMP-9 (P&lt;0.001) without any significant effect on the production of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2.	25-hydroxycholesterol	0-5	matrix metallopeptidase 2	Homo sapiens	64-97
23485764-7	2013	25-HC also significantly enhanced the release and activation of matrix metallaoproteinase (MMP)-2 (P&lt;0.001) and MMP-9 (P&lt;0.001) without any significant effect on the production of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2.	25-hydroxycholesterol	0-5	matrix metallopeptidase 9	Homo sapiens	115-120
23485764-7	2013	25-HC also significantly enhanced the release and activation of matrix metallaoproteinase (MMP)-2 (P&lt;0.001) and MMP-9 (P&lt;0.001) without any significant effect on the production of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2.	25-hydroxycholesterol	0-5	TIMP metallopeptidase inhibitor 1	Homo sapiens	186-232
23485764-7	2013	25-HC also significantly enhanced the release and activation of matrix metallaoproteinase (MMP)-2 (P&lt;0.001) and MMP-9 (P&lt;0.001) without any significant effect on the production of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2.	25-hydroxycholesterol	0-5	TIMP metallopeptidase inhibitor 2	Homo sapiens	237-243
23365445-7	2013	A ligand of OSBP, 25-hydroxycholesterol (25-HC), acted as a minor enviroxime-like compound.	25-hydroxycholesterol	18-39	oxysterol binding protein	Homo sapiens	12-16
23365445-7	2013	A ligand of OSBP, 25-hydroxycholesterol (25-HC), acted as a minor enviroxime-like compound.	25-hydroxycholesterol	41-46	oxysterol binding protein	Homo sapiens	12-16
23347841-0	2013	25-Hydroxycholesterol regulates cholesterol homeostasis in the murine CATH.a neuronal cell line.	25-hydroxycholesterol	0-21	cathepsin H	Mus musculus	70-74
23197320-6	2013	Giving cells lipoproteins or 25-hydroxycholesterol downregulated Ldlr but not Scarb1; Scarb1 was ultimately downregulated by excessive sterol accumulation under 25-hydroxycholesterol and aminoglutethimide (inhibitor of steroidogenesis) cotreatment.	25-hydroxycholesterol	161-182	scavenger receptor class B, member 1	Rattus norvegicus	86-92
23273843-0	2013	The transcription factor STAT-1 couples macrophage synthesis of 25-hydroxycholesterol to the interferon antiviral response.	25-hydroxycholesterol	64-85	signal transducer and activator of transcription 1	Homo sapiens	25-31
23273843-0	2013	The transcription factor STAT-1 couples macrophage synthesis of 25-hydroxycholesterol to the interferon antiviral response.	25-hydroxycholesterol	64-85	interferon alpha 1	Homo sapiens	93-103
23273843-3	2013	Here we report a cellular antiviral role for macrophage production of 25-hydroxycholesterol (cholest-5-en-3beta,25-diol, 25HC) as a component of the sterol metabolic network linked to the IFN response via Stat1.	25-hydroxycholesterol	70-91	interferon alpha 1	Homo sapiens	188-191
23273843-3	2013	Here we report a cellular antiviral role for macrophage production of 25-hydroxycholesterol (cholest-5-en-3beta,25-diol, 25HC) as a component of the sterol metabolic network linked to the IFN response via Stat1.	25-hydroxycholesterol	70-91	signal transducer and activator of transcription 1	Homo sapiens	205-210
23273844-0	2013	Interferon-inducible cholesterol-25-hydroxylase broadly inhibits viral entry by production of 25-hydroxycholesterol.	25-hydroxycholesterol	94-115	cholesterol 25-hydroxylase	Mus musculus	21-47
23273844-3	2013	CH25H converts cholesterol to a soluble antiviral factor, 25-hydroxycholesterol (25HC).	25-hydroxycholesterol	58-79	cholesterol 25-hydroxylase	Mus musculus	0-5
23324282-6	2013	RESULTS: While ABCA7 mRNA was decreased by 25-hydroxycholesterol treatment, ABCA1 was apparently increased.	25-hydroxycholesterol	43-64	ATP binding cassette subfamily A member 7	Homo sapiens	15-20
23239817-7	2013	Notably, we identified decreased expression of the mRNA encoding cholesterol 25-hydroxylase (Ch25h), an enzyme that converts cholesterol to 25-hydroxycholesterol (25HC), an oxysterol with emerging roles in immunity.	25-hydroxycholesterol	140-161	cholesterol 25-hydroxylase	Mus musculus	65-91
23239817-7	2013	Notably, we identified decreased expression of the mRNA encoding cholesterol 25-hydroxylase (Ch25h), an enzyme that converts cholesterol to 25-hydroxycholesterol (25HC), an oxysterol with emerging roles in immunity.	25-hydroxycholesterol	140-161	cholesterol 25-hydroxylase	Mus musculus	93-98
23102786-5	2012	Also, 25-hydroxycholesterol (25HC), an inhibitor of SREBP activation inhibited EL expression.	25-hydroxycholesterol	6-27	lipase G, endothelial type	Homo sapiens	79-81
23102786-5	2012	Also, 25-hydroxycholesterol (25HC), an inhibitor of SREBP activation inhibited EL expression.	25-hydroxycholesterol	29-33	lipase G, endothelial type	Homo sapiens	79-81
22930711-7	2012	By using a hybrid beta(2)-adrenergic receptor-C-X-C chemokine receptor type 4 structure as a template, we created a homology model for EBI2 and optimized the docking of 7alpha,25-OHC into the putative ligand binding site, so that the hydroxyl groups interact with residues Arg87, Asn114, and Glu183.	25-hydroxycholesterol	176-182	G protein-coupled receptor 183	Homo sapiens	135-139
22999953-2	2012	7alpha,25-OHC is synthesized from cholesterol by the stepwise actions of two enzymes, CH25H and CYP7B1, and is metabolized to a 3-oxo derivative by HSD3B7.	25-hydroxycholesterol	7-13	cytochrome P450 family 7 subfamily B member 1	Homo sapiens	96-102
22999953-2	2012	7alpha,25-OHC is synthesized from cholesterol by the stepwise actions of two enzymes, CH25H and CYP7B1, and is metabolized to a 3-oxo derivative by HSD3B7.	25-hydroxycholesterol	7-13	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7	Homo sapiens	148-154
22849850-0	2012	25-Hydroxycholesterol enhances cytokine release and Toll-like receptor 3 response in airway epithelial cells.	25-hydroxycholesterol	0-21	toll like receptor 3	Homo sapiens	52-72
22473958-0	2012	ATF3 protects against atherosclerosis by suppressing 25-hydroxycholesterol-induced lipid body formation.	25-hydroxycholesterol	53-74	activating transcription factor 3	Mus musculus	0-4
22225954-11	2012	SULT2B1b overexpression, combined with administration of 25-hydroxycholesterol, significantly increased the formation of 25HC3S in liver tissue and significantly decreased serum and hepatic lipid levels, including triglycerides, total cholesterol, free cholesterol, and free fatty acids, as compared with controls in both C57BL/6 and LDLR(-/-) mice.	25-hydroxycholesterol	57-78	low density lipoprotein receptor	Mus musculus	334-338
22313893-0	2012	Regulation of the expression of interleukin-8 induced by 25-hydroxycholesterol in retinal pigment epithelium cells.	25-hydroxycholesterol	57-78	C-X-C motif chemokine ligand 8	Homo sapiens	32-45
21979142-4	2012	We found that RIG-I was induced in macrophages and endothelium by 25-hydroxycholesterol.	25-hydroxycholesterol	66-87	DEAD/H box helicase 58	Mus musculus	14-19
21541746-4	2012	Evaluation of the active site residues of the CYP7B1 model and validation of the active site architecture were performed via molecular docking experiments: the docking of the substrates 25-hydroxycholesterol and 27-hydroxycholesterol and the inhibitor 3alpha-Adiol identified structurally and functionally important residues.	25-hydroxycholesterol	186-207	cytochrome P450 family 7 subfamily B member 1	Homo sapiens	46-52
22275753-4	2012	In this paper, we report the effect of 25HC3S and its precursor 25-hydroxycholesterol (25HC) on PPARgamma activity and on inflammatory responses.	25-hydroxycholesterol	64-85	peroxisome proliferator activated receptor gamma	Homo sapiens	96-105
22275753-4	2012	In this paper, we report the effect of 25HC3S and its precursor 25-hydroxycholesterol (25HC) on PPARgamma activity and on inflammatory responses.	25-hydroxycholesterol	39-43	peroxisome proliferator activated receptor gamma	Homo sapiens	96-105
21979142-5	2012	Interferon regulatory factor 1 is a key transcription factor for the induction of RIG-I by 25-hydroxycholesterol.	25-hydroxycholesterol	91-112	interferon regulatory factor 1	Mus musculus	0-30
21979142-5	2012	Interferon regulatory factor 1 is a key transcription factor for the induction of RIG-I by 25-hydroxycholesterol.	25-hydroxycholesterol	91-112	DEAD/H box helicase 58	Mus musculus	82-87
21979142-6	2012	The induction of interleukin-8 and growth-regulated protein alpha, the mouse interleukin-8 homologue, by 25-hydroxycholesterol is mediated by RIG-I signalling.	25-hydroxycholesterol	105-126	chemokine (C-X-C motif) ligand 15	Mus musculus	17-30
21979142-6	2012	The induction of interleukin-8 and growth-regulated protein alpha, the mouse interleukin-8 homologue, by 25-hydroxycholesterol is mediated by RIG-I signalling.	25-hydroxycholesterol	105-126	chemokine (C-X-C motif) ligand 15	Mus musculus	77-90
21979142-6	2012	The induction of interleukin-8 and growth-regulated protein alpha, the mouse interleukin-8 homologue, by 25-hydroxycholesterol is mediated by RIG-I signalling.	25-hydroxycholesterol	105-126	DEAD/H box helicase 58	Mus musculus	142-147
21576599-5	2011	We now report that CYP3A synthesizes a significant amount of 25-hydroxycholesterol and may participate in the regulation of lipid metabolism.	25-hydroxycholesterol	61-82	cytochrome P450 family 3 subfamily A member 4	Homo sapiens	19-24
22582164-1	2011	Cholesterol ester lipase (LIPA; EC 3.1.1.13) and cholesterol 25-hydroxylase (CH25H; EC 1.14.99.48) play essential role in cholesterol metabolism in the body by hydrolysing cholesteryl esters and triglycerides within lysosomes (LIPA) and catalysing the formation of 25-hydroxycholesterol from cholesterol (CH25H) which acts to repress cholesterol biosynthesis.	25-hydroxycholesterol	265-286	lipase A, lysosomal acid type	Homo sapiens	18-24
22582164-1	2011	Cholesterol ester lipase (LIPA; EC 3.1.1.13) and cholesterol 25-hydroxylase (CH25H; EC 1.14.99.48) play essential role in cholesterol metabolism in the body by hydrolysing cholesteryl esters and triglycerides within lysosomes (LIPA) and catalysing the formation of 25-hydroxycholesterol from cholesterol (CH25H) which acts to repress cholesterol biosynthesis.	25-hydroxycholesterol	265-286	lipase A, lysosomal acid type	Homo sapiens	26-30
22582164-1	2011	Cholesterol ester lipase (LIPA; EC 3.1.1.13) and cholesterol 25-hydroxylase (CH25H; EC 1.14.99.48) play essential role in cholesterol metabolism in the body by hydrolysing cholesteryl esters and triglycerides within lysosomes (LIPA) and catalysing the formation of 25-hydroxycholesterol from cholesterol (CH25H) which acts to repress cholesterol biosynthesis.	25-hydroxycholesterol	265-286	cholesterol 25-hydroxylase	Homo sapiens	49-75
22582164-1	2011	Cholesterol ester lipase (LIPA; EC 3.1.1.13) and cholesterol 25-hydroxylase (CH25H; EC 1.14.99.48) play essential role in cholesterol metabolism in the body by hydrolysing cholesteryl esters and triglycerides within lysosomes (LIPA) and catalysing the formation of 25-hydroxycholesterol from cholesterol (CH25H) which acts to repress cholesterol biosynthesis.	25-hydroxycholesterol	265-286	cholesterol 25-hydroxylase	Homo sapiens	77-82
22582164-1	2011	Cholesterol ester lipase (LIPA; EC 3.1.1.13) and cholesterol 25-hydroxylase (CH25H; EC 1.14.99.48) play essential role in cholesterol metabolism in the body by hydrolysing cholesteryl esters and triglycerides within lysosomes (LIPA) and catalysing the formation of 25-hydroxycholesterol from cholesterol (CH25H) which acts to repress cholesterol biosynthesis.	25-hydroxycholesterol	265-286	lipase A, lysosomal acid type	Homo sapiens	227-231
22582164-1	2011	Cholesterol ester lipase (LIPA; EC 3.1.1.13) and cholesterol 25-hydroxylase (CH25H; EC 1.14.99.48) play essential role in cholesterol metabolism in the body by hydrolysing cholesteryl esters and triglycerides within lysosomes (LIPA) and catalysing the formation of 25-hydroxycholesterol from cholesterol (CH25H) which acts to repress cholesterol biosynthesis.	25-hydroxycholesterol	265-286	cholesterol 25-hydroxylase	Homo sapiens	305-310
21669198-8	2011	Similar to ORP7 overexpression, treatment of cells with 25-hydroxycholesterol (25-OH) resulted in GS28 destabilization, which was potentiated by excess ORP7 and inhibited by ORP7 silencing.	25-hydroxycholesterol	56-77	oxysterol binding protein like 7	Homo sapiens	11-15
21669198-8	2011	Similar to ORP7 overexpression, treatment of cells with 25-hydroxycholesterol (25-OH) resulted in GS28 destabilization, which was potentiated by excess ORP7 and inhibited by ORP7 silencing.	25-hydroxycholesterol	56-77	golgi SNAP receptor complex member 1	Homo sapiens	98-102
21669198-8	2011	Similar to ORP7 overexpression, treatment of cells with 25-hydroxycholesterol (25-OH) resulted in GS28 destabilization, which was potentiated by excess ORP7 and inhibited by ORP7 silencing.	25-hydroxycholesterol	56-77	oxysterol binding protein like 7	Homo sapiens	152-156
21669198-8	2011	Similar to ORP7 overexpression, treatment of cells with 25-hydroxycholesterol (25-OH) resulted in GS28 destabilization, which was potentiated by excess ORP7 and inhibited by ORP7 silencing.	25-hydroxycholesterol	56-77	oxysterol binding protein like 7	Homo sapiens	152-156
21669198-8	2011	Similar to ORP7 overexpression, treatment of cells with 25-hydroxycholesterol (25-OH) resulted in GS28 destabilization, which was potentiated by excess ORP7 and inhibited by ORP7 silencing.	25-hydroxycholesterol	79-84	oxysterol binding protein like 7	Homo sapiens	11-15
21669198-8	2011	Similar to ORP7 overexpression, treatment of cells with 25-hydroxycholesterol (25-OH) resulted in GS28 destabilization, which was potentiated by excess ORP7 and inhibited by ORP7 silencing.	25-hydroxycholesterol	79-84	golgi SNAP receptor complex member 1	Homo sapiens	98-102
21669198-8	2011	Similar to ORP7 overexpression, treatment of cells with 25-hydroxycholesterol (25-OH) resulted in GS28 destabilization, which was potentiated by excess ORP7 and inhibited by ORP7 silencing.	25-hydroxycholesterol	79-84	oxysterol binding protein like 7	Homo sapiens	152-156
21669198-8	2011	Similar to ORP7 overexpression, treatment of cells with 25-hydroxycholesterol (25-OH) resulted in GS28 destabilization, which was potentiated by excess ORP7 and inhibited by ORP7 silencing.	25-hydroxycholesterol	79-84	oxysterol binding protein like 7	Homo sapiens	152-156
21576599-6	2011	Induction of CYP3A by pregnenolone-16alpha-carbonitrile caused the accumulation of 25-hydroxycholesterol in a cell line derived from mouse liver.	25-hydroxycholesterol	83-104	cytochrome P450, family 3, subfamily a, polypeptide 11	Mus musculus	13-18
21576599-7	2011	Furthermore, treatment of the cells with troleandomycin, a specific inhibitor of CYP3A, significantly reduced cellular 25-hydroxycholesterol concentrations.	25-hydroxycholesterol	119-140	cytochrome P450 family 3 subfamily A member 4	Homo sapiens	81-86
21576599-10	2011	These results demonstrate the significance of CYP3A on the production of 25-hydroxycholesterol.	25-hydroxycholesterol	73-94	cytochrome P450 family 3 subfamily A member 4	Homo sapiens	46-51
21796211-7	2011	In vitro, 7alpha,25-OHC stimulated the migration of EBI2-expressing mouse B and T cells with half-maximum effective concentration values around 500 pM, but had no effect on EBI2-deficient cells.	25-hydroxycholesterol	17-23	G protein-coupled receptor 183	Mus musculus	52-56
21796211-8	2011	In vivo, EBI2-deficient B cells or normal B cells desensitized by 7alpha,25-OHC pre-treatment showed reduced homing to follicular areas of the spleen.	25-hydroxycholesterol	73-79	G protein-coupled receptor 183	Mus musculus	9-13
21796211-9	2011	Blocking the synthesis of 7alpha,25-OHC in vivo with clotrimazole, a CYP7B1 inhibitor, reduced the content of 7alpha,25-OHC in the mouse spleen and promoted the migration of adoptively transferred pre-activated B cells to the T/B boundary (the boundary between the T-zone and B-zone in the spleen follicle), mimicking the phenotype of pre-activated B cells from EBI2-deficient mice.	25-hydroxycholesterol	33-39	cytochrome P450, family 7, subfamily b, polypeptide 1	Mus musculus	69-75
21796211-9	2011	Blocking the synthesis of 7alpha,25-OHC in vivo with clotrimazole, a CYP7B1 inhibitor, reduced the content of 7alpha,25-OHC in the mouse spleen and promoted the migration of adoptively transferred pre-activated B cells to the T/B boundary (the boundary between the T-zone and B-zone in the spleen follicle), mimicking the phenotype of pre-activated B cells from EBI2-deficient mice.	25-hydroxycholesterol	33-39	G protein-coupled receptor 183	Mus musculus	362-366
21796211-9	2011	Blocking the synthesis of 7alpha,25-OHC in vivo with clotrimazole, a CYP7B1 inhibitor, reduced the content of 7alpha,25-OHC in the mouse spleen and promoted the migration of adoptively transferred pre-activated B cells to the T/B boundary (the boundary between the T-zone and B-zone in the spleen follicle), mimicking the phenotype of pre-activated B cells from EBI2-deficient mice.	25-hydroxycholesterol	117-123	cytochrome P450, family 7, subfamily b, polypeptide 1	Mus musculus	69-75
21796212-4	2011	Functional activation of human EBI2 by 7alpha,25-OHC and closely related oxysterols was verified by monitoring second messenger readouts and saturable, high-affinity radioligand binding.	25-hydroxycholesterol	46-52	G protein-coupled receptor 183	Homo sapiens	31-35
21796212-5	2011	Furthermore, we find that 7alpha,25-OHC and closely related oxysterols act as chemoattractants for immune cells expressing EBI2 by directing cell migration in vitro and in vivo.	25-hydroxycholesterol	33-39	G protein-coupled receptor 183	Homo sapiens	123-127
26124677-4	2009	Here we show that stable expression of RTKN2 in HEK cells enhanced survival in response to intrinsic apoptotic agents; 25-hydroxy cholesterol and camptothecin, but not the extrinsic agent, TNFalpha.	25-hydroxycholesterol	119-141	rhotekin 2	Homo sapiens	39-44
21168770-6	2010	Promoter-targeted duplex RNAs can overcome repression of LDLR expression by 25-hydroxycholesterol and do not interfere with activation of LDLR expression by lovastatin.	25-hydroxycholesterol	76-97	low density lipoprotein receptor	Homo sapiens	57-61
20570710-4	2010	When mevalonate kinase expression was abolished by treating cultured luteal cells with 25-hydroxycholesterol, the ability to undergo LH-induced down regulation of LHR mRNA was completely abrogated.	25-hydroxycholesterol	87-108	mevalonate kinase	Homo sapiens	5-22
20570710-4	2010	When mevalonate kinase expression was abolished by treating cultured luteal cells with 25-hydroxycholesterol, the ability to undergo LH-induced down regulation of LHR mRNA was completely abrogated.	25-hydroxycholesterol	87-108	luteinizing hormone/choriogonadotropin receptor	Homo sapiens	163-166
21070649-0	2010	25-Hydroxycholesterol exerts both a cox-2-dependent transient proliferative effect and cox-2-independent cytotoxic effect on bovine endothelial cells in a time- and cell-type-dependent manner.	25-hydroxycholesterol	0-21	prostaglandin-endoperoxide synthase 2	Bos taurus	36-41
21070649-0	2010	25-Hydroxycholesterol exerts both a cox-2-dependent transient proliferative effect and cox-2-independent cytotoxic effect on bovine endothelial cells in a time- and cell-type-dependent manner.	25-hydroxycholesterol	0-21	prostaglandin-endoperoxide synthase 2	Bos taurus	87-92
21070649-4	2010	RESULTS: We found 25-OHC to be a potent inducer of cyclooxygenase-2 (Cox-2, prostaglandin G-H synthase-2) expression in bovine mesenteric lymphatic, venous, and arterial endothelial cells.	25-hydroxycholesterol	18-24	prostaglandin-endoperoxide synthase 2	Bos taurus	51-67
21070649-4	2010	RESULTS: We found 25-OHC to be a potent inducer of cyclooxygenase-2 (Cox-2, prostaglandin G-H synthase-2) expression in bovine mesenteric lymphatic, venous, and arterial endothelial cells.	25-hydroxycholesterol	18-24	prostaglandin-endoperoxide synthase 2	Bos taurus	69-74
21070649-4	2010	RESULTS: We found 25-OHC to be a potent inducer of cyclooxygenase-2 (Cox-2, prostaglandin G-H synthase-2) expression in bovine mesenteric lymphatic, venous, and arterial endothelial cells.	25-hydroxycholesterol	18-24	prostaglandin-endoperoxide synthase 2	Bos taurus	76-104
21070649-5	2010	The induction of Cox-2 expression in endothelial cells by 25-OHC led to an initial increase in cellular proliferation that was inhibited by the Cox-2 selective inhibitor celecoxib (Celebrex).	25-hydroxycholesterol	58-64	prostaglandin-endoperoxide synthase 2	Bos taurus	17-22
21070649-5	2010	The induction of Cox-2 expression in endothelial cells by 25-OHC led to an initial increase in cellular proliferation that was inhibited by the Cox-2 selective inhibitor celecoxib (Celebrex).	25-hydroxycholesterol	58-64	prostaglandin-endoperoxide synthase 2	Bos taurus	144-149
21070649-7	2010	Furthermore, endothelial cells induced to express Cox-2 by 25-OHC were more sensitive to the effects of the Cox-2 selective inhibitor celecoxib (Celebrex).	25-hydroxycholesterol	59-65	prostaglandin-endoperoxide synthase 2	Bos taurus	50-55
21070649-7	2010	Furthermore, endothelial cells induced to express Cox-2 by 25-OHC were more sensitive to the effects of the Cox-2 selective inhibitor celecoxib (Celebrex).	25-hydroxycholesterol	59-65	prostaglandin-endoperoxide synthase 2	Bos taurus	108-113
21070649-8	2010	These results suggest that some effects of 25-OHC on cells may be dependent on Cox-2 enzymatic activity.	25-hydroxycholesterol	43-49	prostaglandin-endoperoxide synthase 2	Bos taurus	79-84
21070649-9	2010	CONCLUSIONS: Cox-2 dependent elevating effects of 25-OHC on endothelial cell proliferation was transient.	25-hydroxycholesterol	50-56	prostaglandin-endoperoxide synthase 2	Bos taurus	13-18
20700770-4	2010	The present study shows that 25HC3S and its precursor, 25-hydroxycholesterol (25HC), diametrically regulate lipid metabolism and inflammatory response via LXR/SREBP-1 and IkappaBalpha/NFkappaB signaling in hepatocytes.	25-hydroxycholesterol	55-76	sterol regulatory element binding transcription factor 1	Rattus norvegicus	159-166
20700770-4	2010	The present study shows that 25HC3S and its precursor, 25-hydroxycholesterol (25HC), diametrically regulate lipid metabolism and inflammatory response via LXR/SREBP-1 and IkappaBalpha/NFkappaB signaling in hepatocytes.	25-hydroxycholesterol	55-76	NFKB inhibitor alpha	Rattus norvegicus	171-183
20700770-4	2010	The present study shows that 25HC3S and its precursor, 25-hydroxycholesterol (25HC), diametrically regulate lipid metabolism and inflammatory response via LXR/SREBP-1 and IkappaBalpha/NFkappaB signaling in hepatocytes.	25-hydroxycholesterol	29-33	sterol regulatory element binding transcription factor 1	Rattus norvegicus	159-166
20700770-4	2010	The present study shows that 25HC3S and its precursor, 25-hydroxycholesterol (25HC), diametrically regulate lipid metabolism and inflammatory response via LXR/SREBP-1 and IkappaBalpha/NFkappaB signaling in hepatocytes.	25-hydroxycholesterol	29-33	NFKB inhibitor alpha	Rattus norvegicus	171-183
20178991-1	2010	Oxysterol-binding protein (OSBP), a cytosolic receptor of cholesterol and oxysterols, is recruited to the endoplasmic reticulum by binding to the cytoplasmic major sperm protein (MSP) domain of integral endoplasmic reticulum protein VAMP-associated protein-A (VAP-A), a process essential for the stimulation of sphingomyelin synthesis by 25-hydroxycholesterol.	25-hydroxycholesterol	338-359	oxysterol binding protein	Homo sapiens	0-25
20178991-1	2010	Oxysterol-binding protein (OSBP), a cytosolic receptor of cholesterol and oxysterols, is recruited to the endoplasmic reticulum by binding to the cytoplasmic major sperm protein (MSP) domain of integral endoplasmic reticulum protein VAMP-associated protein-A (VAP-A), a process essential for the stimulation of sphingomyelin synthesis by 25-hydroxycholesterol.	25-hydroxycholesterol	338-359	oxysterol binding protein	Homo sapiens	27-31
20178991-1	2010	Oxysterol-binding protein (OSBP), a cytosolic receptor of cholesterol and oxysterols, is recruited to the endoplasmic reticulum by binding to the cytoplasmic major sperm protein (MSP) domain of integral endoplasmic reticulum protein VAMP-associated protein-A (VAP-A), a process essential for the stimulation of sphingomyelin synthesis by 25-hydroxycholesterol.	25-hydroxycholesterol	338-359	microseminoprotein beta	Homo sapiens	179-182
20178991-1	2010	Oxysterol-binding protein (OSBP), a cytosolic receptor of cholesterol and oxysterols, is recruited to the endoplasmic reticulum by binding to the cytoplasmic major sperm protein (MSP) domain of integral endoplasmic reticulum protein VAMP-associated protein-A (VAP-A), a process essential for the stimulation of sphingomyelin synthesis by 25-hydroxycholesterol.	25-hydroxycholesterol	338-359	VAMP associated protein A	Homo sapiens	260-265
19805370-5	2009	Treatment of naive B cells with nanomolar concentrations of 25-hydroxycholesterol suppressed IL-2-mediated stimulation of B cell proliferation, repressed activation-induced cytidine deaminase (AID) expression, and blocked class switch recombination, leading to markedly decreased IgA production.	25-hydroxycholesterol	60-81	interleukin 2	Homo sapiens	93-97
19805370-5	2009	Treatment of naive B cells with nanomolar concentrations of 25-hydroxycholesterol suppressed IL-2-mediated stimulation of B cell proliferation, repressed activation-induced cytidine deaminase (AID) expression, and blocked class switch recombination, leading to markedly decreased IgA production.	25-hydroxycholesterol	60-81	activation induced cytidine deaminase	Homo sapiens	154-191
19805370-5	2009	Treatment of naive B cells with nanomolar concentrations of 25-hydroxycholesterol suppressed IL-2-mediated stimulation of B cell proliferation, repressed activation-induced cytidine deaminase (AID) expression, and blocked class switch recombination, leading to markedly decreased IgA production.	25-hydroxycholesterol	60-81	activation induced cytidine deaminase	Homo sapiens	193-196
20573495-4	2011	Therefore, THP-1 macrophages were exposed to two different oxysterols, such as 7-keto-cholesterol (4-16 muM) and 25-hydroxycholesterol (2-4 muM), alone and in combination with lycopene (0.5-2 muM).	25-hydroxycholesterol	113-134	latexin	Homo sapiens	140-143
20573495-4	2011	Therefore, THP-1 macrophages were exposed to two different oxysterols, such as 7-keto-cholesterol (4-16 muM) and 25-hydroxycholesterol (2-4 muM), alone and in combination with lycopene (0.5-2 muM).	25-hydroxycholesterol	113-134	latexin	Homo sapiens	140-143
21146170-0	2011	Sulfation of 25-hydroxycholesterol by SULT2B1b decreases cellular lipids via the LXR/SREBP-1c signaling pathway in human aortic endothelial cells.	25-hydroxycholesterol	13-34	sterol regulatory element binding transcription factor 1	Homo sapiens	85-93
21304949-0	2011	The cholesterol metabolite 25-hydroxycholesterol activates estrogen receptor alpha-mediated signaling in cancer cells and in cardiomyocytes.	25-hydroxycholesterol	27-48	estrogen receptor 1	Homo sapiens	59-82
21698267-6	2011	ORP8, previously shown to bind 25-hydroxycholesterol, was now shown to bind also cholesterol in vitro.	25-hydroxycholesterol	31-52	oxysterol binding protein like 8	Homo sapiens	0-4
20925360-2	2010	In this study, molecular dynamics simulations were used to investigate the binding of ergosterol, 25-hydroxycholesterol, and lipid moieties to Osh4.	25-hydroxycholesterol	98-119	oxysterol-binding protein KES1	Saccharomyces cerevisiae S288C	143-147
20339855-10	2010	No cytotoxic effects were identified with 25-OH, which highly stimulated ROS production, MCP-1, and VEGF secretion.	25-hydroxycholesterol	42-47	C-C motif chemokine ligand 2	Homo sapiens	89-94
20339855-10	2010	No cytotoxic effects were identified with 25-OH, which highly stimulated ROS production, MCP-1, and VEGF secretion.	25-hydroxycholesterol	42-47	vascular endothelial growth factor A	Homo sapiens	100-104
20339855-12	2010	25-OH induced IL-8 secretion through the MEK/ERK1/2 signaling pathway, and Rsv showed cytoprotective activities and inhibited VEGF secretion.	25-hydroxycholesterol	0-5	C-X-C motif chemokine ligand 8	Homo sapiens	14-18
20339855-12	2010	25-OH induced IL-8 secretion through the MEK/ERK1/2 signaling pathway, and Rsv showed cytoprotective activities and inhibited VEGF secretion.	25-hydroxycholesterol	0-5	mitogen-activated protein kinase kinase 7	Homo sapiens	41-44
20339855-12	2010	25-OH induced IL-8 secretion through the MEK/ERK1/2 signaling pathway, and Rsv showed cytoprotective activities and inhibited VEGF secretion.	25-hydroxycholesterol	0-5	mitogen-activated protein kinase 3	Homo sapiens	45-51
20570635-7	2010	Trophoblasts transfected with ABCA1 or ABCG1 siRNA were more sensitive to toxic oxysterols substrates (25-hydroxycholesterol and 7-ketocholesterol) compared to mock-transfected cells, while prior treatment with T0901317 reduced oxysterol-mediated toxicity.	25-hydroxycholesterol	103-124	ATP binding cassette subfamily A member 1	Homo sapiens	30-35
20570635-7	2010	Trophoblasts transfected with ABCA1 or ABCG1 siRNA were more sensitive to toxic oxysterols substrates (25-hydroxycholesterol and 7-ketocholesterol) compared to mock-transfected cells, while prior treatment with T0901317 reduced oxysterol-mediated toxicity.	25-hydroxycholesterol	103-124	ATP binding cassette subfamily G member 1	Homo sapiens	39-44
20138879-3	2010	25-hydroxycholesterol, a representative LXR activating oxysterol, suppressed IL-6 production and degranulation response in BMMCs following engagement of high-affinity IgE receptor (FcepsilonRI).	25-hydroxycholesterol	0-21	nuclear receptor subfamily 1, group H, member 2	Mus musculus	40-43
20138879-3	2010	25-hydroxycholesterol, a representative LXR activating oxysterol, suppressed IL-6 production and degranulation response in BMMCs following engagement of high-affinity IgE receptor (FcepsilonRI).	25-hydroxycholesterol	0-21	interleukin 6	Mus musculus	77-81
20138879-3	2010	25-hydroxycholesterol, a representative LXR activating oxysterol, suppressed IL-6 production and degranulation response in BMMCs following engagement of high-affinity IgE receptor (FcepsilonRI).	25-hydroxycholesterol	0-21	Fc receptor, IgE, high affinity I, gamma polypeptide	Mus musculus	181-192
20138879-4	2010	Interestingly, 25-hydroxycholesterol reduced cell-surface FcepsilonRI expression by inhibiting assembly of FcepsilonRIalpha and FcepsilonRIbeta.	25-hydroxycholesterol	15-36	Fc receptor, IgE, high affinity I, gamma polypeptide	Mus musculus	58-69
19382214-6	2009	We also report that long-term inhibition of cholesterol biosynthesis with 25-hydroxycholesterol blocks IGF-I stimulated Akt phosphorylation and cell survival.	25-hydroxycholesterol	74-95	insulin like growth factor 1	Homo sapiens	103-108
19382214-6	2009	We also report that long-term inhibition of cholesterol biosynthesis with 25-hydroxycholesterol blocks IGF-I stimulated Akt phosphorylation and cell survival.	25-hydroxycholesterol	74-95	AKT serine/threonine kinase 1	Homo sapiens	120-123
19502589-6	2009	When macrophages were treated with increasing concentrations of 25-hydroxycholesterol, a dose-dependent release of CCL5 into the culture medium was observed.	25-hydroxycholesterol	64-85	chemokine (C-C motif) ligand 5	Mus musculus	115-119
19700418-3	2009	We show that chronic cholesterol depletion achieved with lipoprotein-deficient serum (LPDS) and 25-hydroxycholesterol (25-HC) treatment results in a significant increase in apoptosis in HT-29 and Colo-205 cells containing the (V600E)B-RAF mutation, but not in HCT-116 and LoVo cells harbouring the (G13D)Ras mutation, or BE cells, which possess two mutations, (G13D)Ras and (G463V)B-RAF.	25-hydroxycholesterol	96-117	B-Raf proto-oncogene, serine/threonine kinase	Homo sapiens	233-238
19700418-3	2009	We show that chronic cholesterol depletion achieved with lipoprotein-deficient serum (LPDS) and 25-hydroxycholesterol (25-HC) treatment results in a significant increase in apoptosis in HT-29 and Colo-205 cells containing the (V600E)B-RAF mutation, but not in HCT-116 and LoVo cells harbouring the (G13D)Ras mutation, or BE cells, which possess two mutations, (G13D)Ras and (G463V)B-RAF.	25-hydroxycholesterol	96-117	B-Raf proto-oncogene, serine/threonine kinase	Homo sapiens	381-386
26124677-7	2009	Consistent with the role of RTKN2 in HEK over-expressing cells, suppression of RTKN2 in primary human CD4(+) T-cells reduced viability and increased sensitivity to 25-OHC.	25-hydroxycholesterol	164-170	rhotekin 2	Homo sapiens	79-84
26124677-7	2009	Consistent with the role of RTKN2 in HEK over-expressing cells, suppression of RTKN2 in primary human CD4(+) T-cells reduced viability and increased sensitivity to 25-OHC.	25-hydroxycholesterol	164-170	CD4 molecule	Homo sapiens	102-105
19464253-0	2009	Stimulation of Akt poly-ubiquitination and proteasomal degradation in P388D1 cells by 7-ketocholesterol and 25-hydroxycholesterol.	25-hydroxycholesterol	108-129	thymoma viral proto-oncogene 1	Mus musculus	15-18
19299314-10	2009	Treatment with 25-hydroxycholesterol abrogated the effect of hCG on SREBF1a.	25-hydroxycholesterol	15-36	chorionic gonadotropin subunit beta 5	Homo sapiens	61-64
19464253-2	2009	Previously we observed enhanced degradation of Akt in P388D1 moncocyte/macrophages following treatment with 25-hydroxycholesterol (25-OH) or 7-ketocholesterol (7-KC).	25-hydroxycholesterol	108-129	thymoma viral proto-oncogene 1	Mus musculus	47-50
19464253-2	2009	Previously we observed enhanced degradation of Akt in P388D1 moncocyte/macrophages following treatment with 25-hydroxycholesterol (25-OH) or 7-ketocholesterol (7-KC).	25-hydroxycholesterol	131-136	thymoma viral proto-oncogene 1	Mus musculus	47-50
19464253-4	2009	We show that treatment with 25-OH or 7-KC results in the accumulation of poly-ubiquitinated Akt, an effect that is enhanced by co-treatment with the proteasome inhibitor MG-132.	25-hydroxycholesterol	28-33	thymoma viral proto-oncogene 1	Mus musculus	92-95
19464253-5	2009	Modification of Akt by the addition of a Gly-Ala repeat (GAr), a domain known to block ubiquitin-dependent targeting of proteins to the proteasome, resulted in a chimeric protein that is resistant to turn-over induced by 25-OH or 7-KC and provides protection from apoptosis induced by these oxysterols.	25-hydroxycholesterol	221-226	thymoma viral proto-oncogene 1	Mus musculus	16-19
18980580-3	2009	[(3)H]Choline-labelling experiments showed that 25OH (25-hydroxycholesterol), 22OH (22-hydroxycholesterol) and 27OH (27-hydroxycholesterol) increased PtdCho synthesis in CHO cells as a result of CCTalpha (CTP:phosphocholine cytidylyltransferase alpha) translocation and activation at the NE (nuclear envelope).	25-hydroxycholesterol	48-52	choline-phosphate cytidylyltransferase A	Cricetulus griseus	195-203
19224871-1	2009	Oxysterol binding protein-related protein 2 (ORP2) is a member of the oxysterol binding protein family, previously shown to bind 25-hydroxycholesterol and implicated in cellular cholesterol metabolism.	25-hydroxycholesterol	129-150	oxysterol binding protein like 2	Homo sapiens	0-43
19224871-1	2009	Oxysterol binding protein-related protein 2 (ORP2) is a member of the oxysterol binding protein family, previously shown to bind 25-hydroxycholesterol and implicated in cellular cholesterol metabolism.	25-hydroxycholesterol	129-150	oxysterol binding protein like 2	Homo sapiens	45-49
19563754-4	2009	Here, we describe high-resolution structures of the N-terminal domain (NTD) of NPC1 and complexes with cholesterol and 25-hydroxycholesterol.	25-hydroxycholesterol	119-140	NPC intracellular cholesterol transporter 1	Homo sapiens	79-83
19229075-0	2009	FGF-1 induces expression of LXRalpha and production of 25-hydroxycholesterol to upregulate the apoE gene in rat astrocytes.	25-hydroxycholesterol	55-76	fibroblast growth factor 1	Rattus norvegicus	0-5
19229075-0	2009	FGF-1 induces expression of LXRalpha and production of 25-hydroxycholesterol to upregulate the apoE gene in rat astrocytes.	25-hydroxycholesterol	55-76	apolipoprotein E	Rattus norvegicus	95-99
19229075-26	2009	On the other hand, FGF-1 induced production of 25-hydroxycholesterol by MEK/ERK as an sterol regulatory element-dependent reaction besides cholesterol biosynthesis.	25-hydroxycholesterol	47-68	fibroblast growth factor 1	Rattus norvegicus	19-24
19229075-26	2009	On the other hand, FGF-1 induced production of 25-hydroxycholesterol by MEK/ERK as an sterol regulatory element-dependent reaction besides cholesterol biosynthesis.	25-hydroxycholesterol	47-68	Eph receptor B1	Rattus norvegicus	76-79
19250336-0	2009	25-hydroxycholesterol provokes oligodendrocyte cell line apoptosis and stimulates the secreted phospholipase A2 type IIA via LXR beta and PXR.	25-hydroxycholesterol	0-21	phospholipase A2, group IIA (platelets, synovial fluid)	Mus musculus	86-111
19250336-0	2009	25-hydroxycholesterol provokes oligodendrocyte cell line apoptosis and stimulates the secreted phospholipase A2 type IIA via LXR beta and PXR.	25-hydroxycholesterol	0-21	ATPase, class II, type 9A	Mus musculus	117-120
19250336-0	2009	25-hydroxycholesterol provokes oligodendrocyte cell line apoptosis and stimulates the secreted phospholipase A2 type IIA via LXR beta and PXR.	25-hydroxycholesterol	0-21	nuclear receptor subfamily 1, group H, member 2	Mus musculus	125-133
19250336-0	2009	25-hydroxycholesterol provokes oligodendrocyte cell line apoptosis and stimulates the secreted phospholipase A2 type IIA via LXR beta and PXR.	25-hydroxycholesterol	0-21	nuclear receptor subfamily 1, group I, member 2	Mus musculus	138-141
19250336-7	2009	In fact, 25-OH, 24(S)-OH, and T0901317 stimulated sPLA2-IIA promoter and sPLA2 activity in oligodendrocyte cell line.	25-hydroxycholesterol	9-14	phospholipase A2, group IIA (platelets, synovial fluid)	Mus musculus	50-59
19250336-7	2009	In fact, 25-OH, 24(S)-OH, and T0901317 stimulated sPLA2-IIA promoter and sPLA2 activity in oligodendrocyte cell line.	25-hydroxycholesterol	9-14	phospholipase A2, group IIA (platelets, synovial fluid)	Mus musculus	50-55
19179626-5	2009	Cells treated with 25-hydroxycholesterol (50 microM) also displayed significant reduction in PCSK9 gene (37%, P&lt;0.01) and protein (75% P&lt;0.001) expression, whereas LDLr showed a decrease at the gene (30%, P&lt;0.05) and protein (57%, P&lt;0.01) levels, respectively.	25-hydroxycholesterol	19-40	proprotein convertase subtilisin/kexin type 9	Homo sapiens	93-98
19179626-5	2009	Cells treated with 25-hydroxycholesterol (50 microM) also displayed significant reduction in PCSK9 gene (37%, P&lt;0.01) and protein (75% P&lt;0.001) expression, whereas LDLr showed a decrease at the gene (30%, P&lt;0.05) and protein (57%, P&lt;0.01) levels, respectively.	25-hydroxycholesterol	19-40	low density lipoprotein receptor	Homo sapiens	170-174
18317936-0	2009	7beta-Hydroxycholesterol and 25-hydroxycholesterol-induced interleukin-8 secretion involves a calcium-dependent activation of c-fos via the ERK1/2 signaling pathway in THP-1 cells: oxysterols-induced IL-8 secretion is calcium-dependent.	25-hydroxycholesterol	29-50	C-X-C motif chemokine ligand 8	Homo sapiens	59-72
18317936-0	2009	7beta-Hydroxycholesterol and 25-hydroxycholesterol-induced interleukin-8 secretion involves a calcium-dependent activation of c-fos via the ERK1/2 signaling pathway in THP-1 cells: oxysterols-induced IL-8 secretion is calcium-dependent.	25-hydroxycholesterol	29-50	Fos proto-oncogene, AP-1 transcription factor subunit	Homo sapiens	126-131
18317936-0	2009	7beta-Hydroxycholesterol and 25-hydroxycholesterol-induced interleukin-8 secretion involves a calcium-dependent activation of c-fos via the ERK1/2 signaling pathway in THP-1 cells: oxysterols-induced IL-8 secretion is calcium-dependent.	25-hydroxycholesterol	29-50	mitogen-activated protein kinase 3	Homo sapiens	140-146
18317936-0	2009	7beta-Hydroxycholesterol and 25-hydroxycholesterol-induced interleukin-8 secretion involves a calcium-dependent activation of c-fos via the ERK1/2 signaling pathway in THP-1 cells: oxysterols-induced IL-8 secretion is calcium-dependent.	25-hydroxycholesterol	29-50	C-X-C motif chemokine ligand 8	Homo sapiens	200-204
18317936-3	2009	In this paper, we investigated the signaling pathways leading to the induction of IL-8 secretion in monocytic THP-1 cells treated with 7beta-hydroxycholesterol, a cytototoxic oxysterol, or with 25-hydroxycholesterol, an oxysterol non-cytotoxic toward this cell line.	25-hydroxycholesterol	194-215	C-X-C motif chemokine ligand 8	Homo sapiens	82-86
19531574-4	2009	Treatment of both cell lines with the synthetic LXR ligand T0901317 and oxysterols: 25-hydroxycholesterol (25HC) and 24(S), 25-epoxycholesterol (24,25EC), resulted in more than a 10-fold increase of ABCA1 and ABCG1 mRNA expression.	25-hydroxycholesterol	84-105	ATP binding cassette subfamily A member 1	Homo sapiens	199-204
19531574-4	2009	Treatment of both cell lines with the synthetic LXR ligand T0901317 and oxysterols: 25-hydroxycholesterol (25HC) and 24(S), 25-epoxycholesterol (24,25EC), resulted in more than a 10-fold increase of ABCA1 and ABCG1 mRNA expression.	25-hydroxycholesterol	84-105	ATP binding cassette subfamily G member 1	Homo sapiens	209-214
18980580-5	2009	in vitro, CCTalpha activity was stimulated 2- to 2.5-fold by liposomes containing 5 mol% 25OH, 22OH or 27OH.	25-hydroxycholesterol	89-93	choline-phosphate cytidylyltransferase A	Cricetulus griseus	10-18
18980580-7	2009	25OH activated CCTalpha in CHO-SCAP D443N cells leading to a transient increase in PtdCho synthesis and accumulation of CDP-choline.	25-hydroxycholesterol	0-4	choline-phosphate cytidylyltransferase A	Cricetulus griseus	15-23
18980580-7	2009	25OH activated CCTalpha in CHO-SCAP D443N cells leading to a transient increase in PtdCho synthesis and accumulation of CDP-choline.	25-hydroxycholesterol	0-4	sterol regulatory element-binding protein cleavage-activating protein	Cricetulus griseus	31-35
18980580-8	2009	CCTalpha translocation to the NE and intranuclear tubules in CHO-SCAP D443N cells was complete after 1 h exposure to 25OH compared with only partial translocation by 4-6 h in CHO-Mock cells.	25-hydroxycholesterol	117-121	choline-phosphate cytidylyltransferase A	Cricetulus griseus	0-8
18980580-8	2009	CCTalpha translocation to the NE and intranuclear tubules in CHO-SCAP D443N cells was complete after 1 h exposure to 25OH compared with only partial translocation by 4-6 h in CHO-Mock cells.	25-hydroxycholesterol	117-121	sterol regulatory element-binding protein cleavage-activating protein	Cricetulus griseus	65-69
19239851-6	2009	Cholesterol, 25-hydroxycholesterol, phosphatidylcholine, phosphatidylethanolamine and ceramides are ligands for STARD1/STARD3-6, STARD5, STARD2/STARD10, STARD10 and STARD11, respectively.	25-hydroxycholesterol	13-34	steroidogenic acute regulatory protein	Homo sapiens	112-118
19239851-6	2009	Cholesterol, 25-hydroxycholesterol, phosphatidylcholine, phosphatidylethanolamine and ceramides are ligands for STARD1/STARD3-6, STARD5, STARD2/STARD10, STARD10 and STARD11, respectively.	25-hydroxycholesterol	13-34	StAR related lipid transfer domain containing 3	Homo sapiens	119-127
19239851-6	2009	Cholesterol, 25-hydroxycholesterol, phosphatidylcholine, phosphatidylethanolamine and ceramides are ligands for STARD1/STARD3-6, STARD5, STARD2/STARD10, STARD10 and STARD11, respectively.	25-hydroxycholesterol	13-34	StAR related lipid transfer domain containing 5	Homo sapiens	129-135
19239851-6	2009	Cholesterol, 25-hydroxycholesterol, phosphatidylcholine, phosphatidylethanolamine and ceramides are ligands for STARD1/STARD3-6, STARD5, STARD2/STARD10, STARD10 and STARD11, respectively.	25-hydroxycholesterol	13-34	phosphatidylcholine transfer protein	Homo sapiens	137-143
19239851-6	2009	Cholesterol, 25-hydroxycholesterol, phosphatidylcholine, phosphatidylethanolamine and ceramides are ligands for STARD1/STARD3-6, STARD5, STARD2/STARD10, STARD10 and STARD11, respectively.	25-hydroxycholesterol	13-34	StAR related lipid transfer domain containing 10	Homo sapiens	144-151
19239851-6	2009	Cholesterol, 25-hydroxycholesterol, phosphatidylcholine, phosphatidylethanolamine and ceramides are ligands for STARD1/STARD3-6, STARD5, STARD2/STARD10, STARD10 and STARD11, respectively.	25-hydroxycholesterol	13-34	StAR related lipid transfer domain containing 10	Homo sapiens	153-160
19239851-6	2009	Cholesterol, 25-hydroxycholesterol, phosphatidylcholine, phosphatidylethanolamine and ceramides are ligands for STARD1/STARD3-6, STARD5, STARD2/STARD10, STARD10 and STARD11, respectively.	25-hydroxycholesterol	13-34	ceramide transporter 1	Homo sapiens	165-172
18980580-3	2009	[(3)H]Choline-labelling experiments showed that 25OH (25-hydroxycholesterol), 22OH (22-hydroxycholesterol) and 27OH (27-hydroxycholesterol) increased PtdCho synthesis in CHO cells as a result of CCTalpha (CTP:phosphocholine cytidylyltransferase alpha) translocation and activation at the NE (nuclear envelope).	25-hydroxycholesterol	48-52	choline-phosphate cytidylyltransferase A	Cricetulus griseus	205-250
18450749-9	2008	Sphingomyelin synthesis was suppressed in OSBP-depleted cells treated with 25-hydroxycholesterol but not TO901317 or 22-hydroxycholesterol and did not correlate with ABCA1 stabilization.	25-hydroxycholesterol	75-96	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	42-46
18854425-4	2008	We have now studied the effects of 25HC3S and its precursor, 25-hydroxycholesterol (25HC), on lipid metabolism as mediated by the LXR/SREBP-1 signaling in macrophages.	25-hydroxycholesterol	61-82	sterol regulatory element binding transcription factor 1	Homo sapiens	134-141
18040592-5	2008	Incubation of testicular tissues with 100 IU/ml human chorionic gonadotropin (hCG) and 5 mM dibutyryl-cAMP (dbcAMP), which activate rate-limiting steps in steroid synthesis, or 1.3 microM 25-hydroxycholesterol (25-OHC), which augments production, resulted in significant increases in steroidogenesis over controls.	25-hydroxycholesterol	188-209	chorionic gonadotropin subunit beta 5	Homo sapiens	78-81
18040592-5	2008	Incubation of testicular tissues with 100 IU/ml human chorionic gonadotropin (hCG) and 5 mM dibutyryl-cAMP (dbcAMP), which activate rate-limiting steps in steroid synthesis, or 1.3 microM 25-hydroxycholesterol (25-OHC), which augments production, resulted in significant increases in steroidogenesis over controls.	25-hydroxycholesterol	211-217	chorionic gonadotropin subunit beta 5	Homo sapiens	78-81
22820672-1	2008	Metabolic pulse-chase experiments demonstrated that 25-hydroxycholesterol (25-OH), the endogenous activator of the liver X receptor (LXR), significantly reduced the biosynthesis of phosphatidylethanolamine via CDP-ethanolamine (Kennedy) pathway at the step catalyzed by CTP: phosphoethanolamine cytidylyltransferase (Pcyt2).	25-hydroxycholesterol	52-73	nuclear receptor subfamily 1, group H, member 3	Mus musculus	115-131
18569012-0	2008	25-hydroxycholesterol induces mitochondria-dependent apoptosis via activation of glycogen synthase kinase-3beta in PC12 cells.	25-hydroxycholesterol	0-21	glycogen synthase kinase 3 beta	Rattus norvegicus	81-111
18165705-8	2008	Even though 25-hydroxycholesterol will compete for cholesterol binding to OSBP(408-809), it will not compete for cholesterol binding in full-length OSBP.	25-hydroxycholesterol	12-33	oxysterol binding protein	Homo sapiens	74-78
17991739-3	2008	ORP8 is localized in the endoplasmic reticulum (ER) via its C-terminal transmembrane span and binds 25-hydroxycholesterol, identifying it as a new ER oxysterol-binding protein.	25-hydroxycholesterol	100-121	oxysterol binding protein like 8	Homo sapiens	0-4
22820672-1	2008	Metabolic pulse-chase experiments demonstrated that 25-hydroxycholesterol (25-OH), the endogenous activator of the liver X receptor (LXR), significantly reduced the biosynthesis of phosphatidylethanolamine via CDP-ethanolamine (Kennedy) pathway at the step catalyzed by CTP: phosphoethanolamine cytidylyltransferase (Pcyt2).	25-hydroxycholesterol	52-73	nuclear receptor subfamily 1, group H, member 3	Mus musculus	133-136
22820672-1	2008	Metabolic pulse-chase experiments demonstrated that 25-hydroxycholesterol (25-OH), the endogenous activator of the liver X receptor (LXR), significantly reduced the biosynthesis of phosphatidylethanolamine via CDP-ethanolamine (Kennedy) pathway at the step catalyzed by CTP: phosphoethanolamine cytidylyltransferase (Pcyt2).	25-hydroxycholesterol	52-73	phosphate cytidylyltransferase 2, ethanolamine	Mus musculus	270-315
22820672-1	2008	Metabolic pulse-chase experiments demonstrated that 25-hydroxycholesterol (25-OH), the endogenous activator of the liver X receptor (LXR), significantly reduced the biosynthesis of phosphatidylethanolamine via CDP-ethanolamine (Kennedy) pathway at the step catalyzed by CTP: phosphoethanolamine cytidylyltransferase (Pcyt2).	25-hydroxycholesterol	52-73	phosphate cytidylyltransferase 2, ethanolamine	Mus musculus	317-322
22820672-1	2008	Metabolic pulse-chase experiments demonstrated that 25-hydroxycholesterol (25-OH), the endogenous activator of the liver X receptor (LXR), significantly reduced the biosynthesis of phosphatidylethanolamine via CDP-ethanolamine (Kennedy) pathway at the step catalyzed by CTP: phosphoethanolamine cytidylyltransferase (Pcyt2).	25-hydroxycholesterol	75-80	nuclear receptor subfamily 1, group H, member 3	Mus musculus	115-131
22820672-1	2008	Metabolic pulse-chase experiments demonstrated that 25-hydroxycholesterol (25-OH), the endogenous activator of the liver X receptor (LXR), significantly reduced the biosynthesis of phosphatidylethanolamine via CDP-ethanolamine (Kennedy) pathway at the step catalyzed by CTP: phosphoethanolamine cytidylyltransferase (Pcyt2).	25-hydroxycholesterol	75-80	nuclear receptor subfamily 1, group H, member 3	Mus musculus	133-136
22820672-1	2008	Metabolic pulse-chase experiments demonstrated that 25-hydroxycholesterol (25-OH), the endogenous activator of the liver X receptor (LXR), significantly reduced the biosynthesis of phosphatidylethanolamine via CDP-ethanolamine (Kennedy) pathway at the step catalyzed by CTP: phosphoethanolamine cytidylyltransferase (Pcyt2).	25-hydroxycholesterol	75-80	phosphate cytidylyltransferase 2, ethanolamine	Mus musculus	270-315
22820672-1	2008	Metabolic pulse-chase experiments demonstrated that 25-hydroxycholesterol (25-OH), the endogenous activator of the liver X receptor (LXR), significantly reduced the biosynthesis of phosphatidylethanolamine via CDP-ethanolamine (Kennedy) pathway at the step catalyzed by CTP: phosphoethanolamine cytidylyltransferase (Pcyt2).	25-hydroxycholesterol	75-80	phosphate cytidylyltransferase 2, ethanolamine	Mus musculus	317-322
17550979-7	2007	Because Mvk expression is regulated by sterol response element-binding protein-1, which is sensitive to the cellular concentration of 25-hydroxycholesterol (25-OHC), cultured granulosa cells were treated with this oxysterol, and the expression of Mvk gene was examined.	25-hydroxycholesterol	134-155	mevalonate kinase	Homo sapiens	8-11
17890683-5	2007	Assays using mitochondria isolated from rats and sterol 27-hydroxylase (Cyp27A1) gene knockout mice indicated that 25-hydroxycholesterol (25HC) is synthesized by CYP27A1.	25-hydroxycholesterol	115-136	cytochrome P450, family 27, subfamily a, polypeptide 1	Rattus norvegicus	49-70
17890683-5	2007	Assays using mitochondria isolated from rats and sterol 27-hydroxylase (Cyp27A1) gene knockout mice indicated that 25-hydroxycholesterol (25HC) is synthesized by CYP27A1.	25-hydroxycholesterol	115-136	cytochrome P450, family 27, subfamily a, polypeptide 1	Rattus norvegicus	72-79
17890683-5	2007	Assays using mitochondria isolated from rats and sterol 27-hydroxylase (Cyp27A1) gene knockout mice indicated that 25-hydroxycholesterol (25HC) is synthesized by CYP27A1.	25-hydroxycholesterol	115-136	cytochrome P450, family 27, subfamily a, polypeptide 1	Mus musculus	162-169
17890683-5	2007	Assays using mitochondria isolated from rats and sterol 27-hydroxylase (Cyp27A1) gene knockout mice indicated that 25-hydroxycholesterol (25HC) is synthesized by CYP27A1.	25-hydroxycholesterol	138-142	cytochrome P450, family 27, subfamily a, polypeptide 1	Rattus norvegicus	49-70
17890683-5	2007	Assays using mitochondria isolated from rats and sterol 27-hydroxylase (Cyp27A1) gene knockout mice indicated that 25-hydroxycholesterol (25HC) is synthesized by CYP27A1.	25-hydroxycholesterol	138-142	cytochrome P450, family 27, subfamily a, polypeptide 1	Rattus norvegicus	72-79
17890683-5	2007	Assays using mitochondria isolated from rats and sterol 27-hydroxylase (Cyp27A1) gene knockout mice indicated that 25-hydroxycholesterol (25HC) is synthesized by CYP27A1.	25-hydroxycholesterol	138-142	cytochrome P450, family 27, subfamily a, polypeptide 1	Mus musculus	162-169
17624300-8	2007	In contrast, 25-hydroxycholesterol increased SREBP1 and FAS mRNA levels in primary human hepatocytes.	25-hydroxycholesterol	13-34	sterol regulatory element binding transcription factor 1	Homo sapiens	45-51
17624300-8	2007	In contrast, 25-hydroxycholesterol increased SREBP1 and FAS mRNA levels in primary human hepatocytes.	25-hydroxycholesterol	13-34	fatty acid synthase	Homo sapiens	56-59
17586788-7	2007	Our data indicate that overproduced SCAP saturates the remaining Insig-2 in SRD-19 cells, thus explaining their resistance to 25-hydroxycholesterol.	25-hydroxycholesterol	126-147	sterol regulatory element-binding protein cleavage-activating protein	Cricetulus griseus	36-40
17550979-7	2007	Because Mvk expression is regulated by sterol response element-binding protein-1, which is sensitive to the cellular concentration of 25-hydroxycholesterol (25-OHC), cultured granulosa cells were treated with this oxysterol, and the expression of Mvk gene was examined.	25-hydroxycholesterol	157-163	mevalonate kinase	Homo sapiens	8-11
17550979-8	2007	As expected, treatment with 25-OHC inhibited the Mvk (LRBP) expression, as well as the LHR mRNA binding activity of LRBP.	25-hydroxycholesterol	28-34	mevalonate kinase	Homo sapiens	49-52
17550979-8	2007	As expected, treatment with 25-OHC inhibited the Mvk (LRBP) expression, as well as the LHR mRNA binding activity of LRBP.	25-hydroxycholesterol	28-34	mevalonate kinase	Homo sapiens	54-58
17550979-8	2007	As expected, treatment with 25-OHC inhibited the Mvk (LRBP) expression, as well as the LHR mRNA binding activity of LRBP.	25-hydroxycholesterol	28-34	luteinizing hormone/choriogonadotropin receptor	Homo sapiens	87-90
17550979-8	2007	As expected, treatment with 25-OHC inhibited the Mvk (LRBP) expression, as well as the LHR mRNA binding activity of LRBP.	25-hydroxycholesterol	28-34	mevalonate kinase	Homo sapiens	116-120
17550979-9	2007	To determine the role of Mvk in ligand-mediated down-regulation of LHR mRNA, cells were additionally treated with 25-OHC when treated with hCG.	25-hydroxycholesterol	114-120	hypertrichosis 2 (generalised, congenital)	Homo sapiens	139-142
17453947-11	2007	They also enhanced IL-8 gene expression and IL-8 protein secretion in the following decreasing order: 25-hydroxycholesterol &gt; 24-hydroxycholesterol &gt; 7-ketocholesterol.	25-hydroxycholesterol	102-123	C-X-C motif chemokine ligand 8	Homo sapiens	19-23
17428193-0	2007	The mammalian oxysterol-binding protein-related proteins (ORPs) bind 25-hydroxycholesterol in an evolutionarily conserved pocket.	25-hydroxycholesterol	69-90	oxysterol binding protein	Homo sapiens	14-39
17428193-2	2007	We employed an in vitro [3H]25OH (25-hydroxycholesterol)-binding assay with purified recombinant proteins as well as live cell photo-cross-linking with [3H]photo-25OH and [3H]photoCH (photo-cholesterol), to investigate sterol binding by the mammalian ORPs.	25-hydroxycholesterol	34-55	cholesterol 25-hydroxylase	Homo sapiens	26-32
17453947-11	2007	They also enhanced IL-8 gene expression and IL-8 protein secretion in the following decreasing order: 25-hydroxycholesterol &gt; 24-hydroxycholesterol &gt; 7-ketocholesterol.	25-hydroxycholesterol	102-123	C-X-C motif chemokine ligand 8	Homo sapiens	44-48
16647063-4	2006	However, the enzymatic activity of H386A-ACAT1 was restored to &lt;37% of the level of the wild-type activity when cholesterol was replaced by 25-hydroxycholesterol as substrate.	25-hydroxycholesterol	143-164	acetyl-CoA acetyltransferase 1	Homo sapiens	41-46
17364953-0	2007	25-Hydroxycholesterol, 7beta-hydroxycholesterol and 7-ketocholesterol upregulate interleukin-8 expression independently of Toll-like receptor 1, 2, 4 or 6 signalling in human macrophages.	25-hydroxycholesterol	0-21	C-X-C motif chemokine ligand 8	Homo sapiens	81-94
17038318-7	2006	25-Hydroxycholesterol, a potent inhibitor of the LDL receptor pathway, down-regulated LDL degradation in CHO7 cells only in part and did not down-regulate HDL degradation.	25-hydroxycholesterol	0-21	low-density lipoprotein receptor	Cricetulus griseus	49-61
16571669-4	2006	RNA interference (RNAi) experiments in Chinese hamster ovary (CHO)-K1 cells revealed that OSBP and vesicle-associated membrane protein-associated protein (VAP) were required for stimulation of CERT-dependent ceramide transport and SM synthesis by 25-hydroxycholesterol and cholesterol depletion in response to cyclodextrin.	25-hydroxycholesterol	247-268	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	90-94
16571669-4	2006	RNA interference (RNAi) experiments in Chinese hamster ovary (CHO)-K1 cells revealed that OSBP and vesicle-associated membrane protein-associated protein (VAP) were required for stimulation of CERT-dependent ceramide transport and SM synthesis by 25-hydroxycholesterol and cholesterol depletion in response to cyclodextrin.	25-hydroxycholesterol	247-268	ceramide transfer protein	Cricetulus griseus	193-197
17156886-3	2007	METHODS: Expression and activity of LDLR were assessed by RT-PCR and LDL entry, in the absence or presence of squalestatin or 25-hydroxycholesterol that up- or down-regulates LDLR expression, respectively.	25-hydroxycholesterol	126-147	low density lipoprotein receptor	Homo sapiens	175-179
17156886-8	2007	In hepatocytes pre-treated with squalestatin or 25-hydroxycholesterol before infection, viral RNA accumulation increased or decreased in parallel with LDLR mRNA expression and LDL entry.	25-hydroxycholesterol	48-69	low density lipoprotein receptor	Homo sapiens	151-155
17142457-5	2007	In HepG2 cells, co-administration of a potent suppressor of SREBP-2 activation, 25-hydroxycholesterol, inhibits CYP4F2 mRNA induction by lovastatin.	25-hydroxycholesterol	80-101	sterol regulatory element binding transcription factor 2	Homo sapiens	60-67
17142457-5	2007	In HepG2 cells, co-administration of a potent suppressor of SREBP-2 activation, 25-hydroxycholesterol, inhibits CYP4F2 mRNA induction by lovastatin.	25-hydroxycholesterol	80-101	cytochrome P450 family 4 subfamily F member 2	Homo sapiens	112-118
17161435-0	2007	Up-regulation of skeletal muscle LIM protein 1 gene by 25-hydroxycholesterol may mediate morphological changes of rat aortic smooth muscle cells.	25-hydroxycholesterol	55-76	four and a half LIM domains 1	Rattus norvegicus	17-46
17161435-1	2007	Changes in the expression level of the skeletal muscle LIM protein 1 (SLIM1) in cultured A10 cells were monitored in response to 25-hydroxycholesterol (25-HC), an oxidized form of cholesterol present in the oxidized low-density lipoproteins.	25-hydroxycholesterol	129-150	four and a half LIM domains 1	Rattus norvegicus	39-68
17161435-1	2007	Changes in the expression level of the skeletal muscle LIM protein 1 (SLIM1) in cultured A10 cells were monitored in response to 25-hydroxycholesterol (25-HC), an oxidized form of cholesterol present in the oxidized low-density lipoproteins.	25-hydroxycholesterol	129-150	four and a half LIM domains 1	Rattus norvegicus	70-75
17008555-3	2007	We demonstrated that human NPC1L1 mRNA expression was significantly decreased by 25-hydroxycholesterol but increased in response to cellular cholesterol depletion achieved by incubation with Mevinolin (an inhibitor of 3-hydroxy-3-methylglutaryl-CoA reductase) in human intestinal Caco-2 cells.	25-hydroxycholesterol	81-102	NPC1 like intracellular cholesterol transporter 1	Homo sapiens	27-33
17008555-4	2007	We also showed that a -1741/+56 fragment of the NPC1L1 gene demonstrated high promoter activity in Caco-2 cells that was reduced by 25-hydroxycholesterol and stimulated by cholesterol depletion.	25-hydroxycholesterol	132-153	NPC1 like intracellular cholesterol transporter 1	Homo sapiens	48-54
17560160-11	2007	Mevalonate cycle inhibiting fluvastatin and 25-hydroxycholesterol decreased cholesterol production in leptin-stimulated monocytes.	25-hydroxycholesterol	44-65	leptin	Homo sapiens	102-108
16611739-0	2006	ABCA1 mediates high-affinity uptake of 25-hydroxycholesterol by membrane vesicles and rapid efflux of oxysterol by intact cells.	25-hydroxycholesterol	39-60	ATP binding cassette subfamily A member 1	Homo sapiens	0-5
16611739-5	2006	Comparison of wild-type and ABCA1(-/-) murine fibroblasts indicates that 25-hydroxycholesterol is effluxed approximately 5-fold more rapidly by wild-type cells.	25-hydroxycholesterol	73-94	ATP-binding cassette, sub-family A (ABC1), member 1	Mus musculus	28-33
16699960-3	2006	We show that chronic cholesterol depletion achieved with lipoprotein-deficient serum (LPDS) and 25-hydroxycholesterol (25-HC) treatment resulted in a significant increase in cellular apoptosis and caspase-3 activation.	25-hydroxycholesterol	96-117	caspase 3	Mus musculus	197-206
16696936-2	2006	Cholesterol 25-hydroxylase (CH25H) is an enzyme converting cholesterol into 25-hydroxycholesterol.	25-hydroxycholesterol	76-97	cholesterol 25-hydroxylase	Homo sapiens	0-26
16696936-2	2006	Cholesterol 25-hydroxylase (CH25H) is an enzyme converting cholesterol into 25-hydroxycholesterol.	25-hydroxycholesterol	76-97	cholesterol 25-hydroxylase	Homo sapiens	28-33
16405739-8	2006	In addition, 25-hydroxycholesterol was found to contribute to the transcriptional regulation of hTERT gene.	25-hydroxycholesterol	13-34	telomerase reverse transcriptase	Homo sapiens	96-101
16516145-0	2006	Cholesterol, LDL, and 25-hydroxycholesterol regulate expression of the steroidogenic acute regulatory protein in microvascular endothelial cell line (bEnd.3).	25-hydroxycholesterol	22-43	steroidogenic acute regulatory protein	Mus musculus	71-101
16516145-7	2006	In contrast to CHO and LDL, 25-OH increased StAR protein levels independently of mRNA amount.	25-hydroxycholesterol	28-33	steroidogenic acute regulatory protein	Mus musculus	44-48
16260115-8	2006	Bcl-2 mRNA level in the Sertoli cells decreased by 60% after 24 h exposure to 25-hydroxycholesterol.	25-hydroxycholesterol	78-99	BCL2, apoptosis regulator	Rattus norvegicus	0-5
16260115-9	2006	In parallel, Bax mRNA level increased by 40% in the Sertoli cells incubated in presence of 50 microM of 25-hydroxycholesterol.	25-hydroxycholesterol	104-125	BCL2 associated X, apoptosis regulator	Rattus norvegicus	13-16
17086498-0	2005	Combined effect of 25-hydroxycholesterol and IL-1beta on IL-8 production in human colon carcinoma cell line (Caco-2).	25-hydroxycholesterol	19-40	C-X-C motif chemokine ligand 8	Homo sapiens	57-61
17086498-4	2005	Thus, we investigated the effect of oxysterols, including 25-hydroxycholesterol and 7beta-hydroxycholesterol, on IL-1beta-induced IL-8 production in Caco-2 cells (a human colon carcinoma cell line).	25-hydroxycholesterol	58-79	interleukin 1 beta	Homo sapiens	113-121
17086498-5	2005	Pre-treatment of Caco-2 cells with 25-hydroxycholesterol significantly enhanced IL-1beta-induced IL-8 expression at both mRNA and protein levels.	25-hydroxycholesterol	35-56	interleukin 1 beta	Homo sapiens	80-88
17086498-5	2005	Pre-treatment of Caco-2 cells with 25-hydroxycholesterol significantly enhanced IL-1beta-induced IL-8 expression at both mRNA and protein levels.	25-hydroxycholesterol	35-56	C-X-C motif chemokine ligand 8	Homo sapiens	97-101
17086498-7	2005	Furthermore, pre-treatment with 25-hydroxycholesterol, followed by IL-1beta stimulation, enhanced IL-8 promoter activity beyond that observed with IL-1beta alone.	25-hydroxycholesterol	32-53	C-X-C motif chemokine ligand 8	Homo sapiens	98-102
17086498-7	2005	Furthermore, pre-treatment with 25-hydroxycholesterol, followed by IL-1beta stimulation, enhanced IL-8 promoter activity beyond that observed with IL-1beta alone.	25-hydroxycholesterol	32-53	interleukin 1 beta	Homo sapiens	147-155
17086498-8	2005	These results suggest that 25-hydroxycholesterol enhances IL-1beta-induced IL-8 production, possibly by enhancing promoter activity.	25-hydroxycholesterol	27-48	interleukin 1 beta	Homo sapiens	58-66
17086498-8	2005	These results suggest that 25-hydroxycholesterol enhances IL-1beta-induced IL-8 production, possibly by enhancing promoter activity.	25-hydroxycholesterol	27-48	C-X-C motif chemokine ligand 8	Homo sapiens	75-79
15897605-9	2005	The ability of StarD5 to bind not only cholesterol but also 25-hydroxycholesterol, a potent inflammatory mediator and regulatory oxysterol, raises basic fundamental questions about StarD5"s role in the maintenance of cellular cholesterol homeostasis.	25-hydroxycholesterol	60-81	StAR related lipid transfer domain containing 5	Homo sapiens	15-21
15897605-7	2005	In contrast to selective cholesterol binding by StarD1, StarD5 bound the potent regulatory oxysterol, 25-hydroxycholesterol, in a concentration-dependent manner.	25-hydroxycholesterol	102-123	StAR related lipid transfer domain containing 5	Homo sapiens	56-62
15897605-8	2005	StarD5 binding appeared selective for cholesterol and 25-hydroxycholesterol, as no binding was observed for other tested sterols.	25-hydroxycholesterol	54-75	StAR related lipid transfer domain containing 5	Homo sapiens	0-6
16251608-3	2005	Incubation of fibroblasts and HepG2 cells in serum-free medium supplemented with 25-hydroxycholesterol (25OH-cholesterol, 5 mg/L) for 24 h decreased LDLr protein and mRNA levels by 50-90% (P &lt; 0.05).	25-hydroxycholesterol	81-102	low density lipoprotein receptor	Homo sapiens	149-153
15823271-2	2005	In the present study, using human aortic endothelial cells (HAECs), we investigated whether a model compound for oxysterols, 25-hydroxycholesterol, can enhance the monocyte adherence to HAECs exposed to 25-hydroxycholesterol via increasing expression of vascular cell adhesion molecule-1 (VCAM-1).	25-hydroxycholesterol	125-146	vascular cell adhesion molecule 1	Homo sapiens	254-287
15899885-4	2005	We show that addition of cholesterol or 25-hydroxycholesterol to microsomal membranes in vitro blocks Sar1-dependent binding of the Sec23/24 complex to Scap, the SREBP escort protein.	25-hydroxycholesterol	40-61	secretion associated Ras related GTPase 1A	Homo sapiens	102-106
15899885-4	2005	We show that addition of cholesterol or 25-hydroxycholesterol to microsomal membranes in vitro blocks Sar1-dependent binding of the Sec23/24 complex to Scap, the SREBP escort protein.	25-hydroxycholesterol	40-61	SREBF chaperone	Homo sapiens	152-156
15976441-3	2005	Cholesterol, 25-hydroxycholesterol, phosphatidylcholine, phosphatidylethanolamine and ceramides are ligands for STARD1/STARD3/STARD5, STARD5, STARD2/STARD10, STARD10 and STARD11, respectively.	25-hydroxycholesterol	13-34	steroidogenic acute regulatory protein	Homo sapiens	112-118
15976441-3	2005	Cholesterol, 25-hydroxycholesterol, phosphatidylcholine, phosphatidylethanolamine and ceramides are ligands for STARD1/STARD3/STARD5, STARD5, STARD2/STARD10, STARD10 and STARD11, respectively.	25-hydroxycholesterol	13-34	StAR related lipid transfer domain containing 3	Homo sapiens	119-125
15976441-3	2005	Cholesterol, 25-hydroxycholesterol, phosphatidylcholine, phosphatidylethanolamine and ceramides are ligands for STARD1/STARD3/STARD5, STARD5, STARD2/STARD10, STARD10 and STARD11, respectively.	25-hydroxycholesterol	13-34	StAR related lipid transfer domain containing 5	Homo sapiens	126-132
15976441-3	2005	Cholesterol, 25-hydroxycholesterol, phosphatidylcholine, phosphatidylethanolamine and ceramides are ligands for STARD1/STARD3/STARD5, STARD5, STARD2/STARD10, STARD10 and STARD11, respectively.	25-hydroxycholesterol	13-34	StAR related lipid transfer domain containing 5	Homo sapiens	134-140
15976441-3	2005	Cholesterol, 25-hydroxycholesterol, phosphatidylcholine, phosphatidylethanolamine and ceramides are ligands for STARD1/STARD3/STARD5, STARD5, STARD2/STARD10, STARD10 and STARD11, respectively.	25-hydroxycholesterol	13-34	phosphatidylcholine transfer protein	Homo sapiens	142-148
15976441-3	2005	Cholesterol, 25-hydroxycholesterol, phosphatidylcholine, phosphatidylethanolamine and ceramides are ligands for STARD1/STARD3/STARD5, STARD5, STARD2/STARD10, STARD10 and STARD11, respectively.	25-hydroxycholesterol	13-34	StAR related lipid transfer domain containing 10	Homo sapiens	149-156
15976441-3	2005	Cholesterol, 25-hydroxycholesterol, phosphatidylcholine, phosphatidylethanolamine and ceramides are ligands for STARD1/STARD3/STARD5, STARD5, STARD2/STARD10, STARD10 and STARD11, respectively.	25-hydroxycholesterol	13-34	StAR related lipid transfer domain containing 10	Homo sapiens	158-165
15976441-3	2005	Cholesterol, 25-hydroxycholesterol, phosphatidylcholine, phosphatidylethanolamine and ceramides are ligands for STARD1/STARD3/STARD5, STARD5, STARD2/STARD10, STARD10 and STARD11, respectively.	25-hydroxycholesterol	13-34	ceramide transporter 1	Homo sapiens	170-177
15823271-2	2005	In the present study, using human aortic endothelial cells (HAECs), we investigated whether a model compound for oxysterols, 25-hydroxycholesterol, can enhance the monocyte adherence to HAECs exposed to 25-hydroxycholesterol via increasing expression of vascular cell adhesion molecule-1 (VCAM-1).	25-hydroxycholesterol	125-146	vascular cell adhesion molecule 1	Homo sapiens	289-295
15823271-4	2005	We found that 25-hydroxycholesterol enhances surface expression determined by ELISA, induces VCAM-1 mRNA expression by real time-PCR, and stimulates adhesiveness of HAECs to U937 monocytic cells in a dose-dependent fashion.	25-hydroxycholesterol	14-35	vascular cell adhesion molecule 1	Homo sapiens	93-99
15823271-5	2005	The combination treatment with anti-VCAM-1 and anti-CD11b monoclonal antibodies significantly reduced the monocyte adherence to 25-hydroxycholesterol-stimulated HAECs.	25-hydroxycholesterol	128-149	vascular cell adhesion molecule 1	Homo sapiens	36-42
15823271-5	2005	The combination treatment with anti-VCAM-1 and anti-CD11b monoclonal antibodies significantly reduced the monocyte adherence to 25-hydroxycholesterol-stimulated HAECs.	25-hydroxycholesterol	128-149	integrin subunit alpha M	Homo sapiens	52-57
15452130-0	2004	Cholesterol and 25-hydroxycholesterol inhibit activation of SREBPs by different mechanisms, both involving SCAP and Insigs.	25-hydroxycholesterol	16-37	sterol regulatory element-binding protein cleavage-activating protein	Cricetulus griseus	107-111
15250942-0	2004	25-hydroxycholesterol acts in the Golgi compartment to induce degradation of tyrosinase.	25-hydroxycholesterol	0-21	tyrosinase	Mus musculus	77-87
15249218-5	2004	Cholesterol and 25-hydroxycholesterol decreased the CYP8B1/luciferase reporter activity.	25-hydroxycholesterol	16-37	cytochrome P450 family 8 subfamily B member 1	Rattus norvegicus	52-58
15317807-0	2004	Effects of 25-hydroxycholesterol on cholesterol esterification and sterol regulatory element-binding protein processing are dissociable: implications for cholesterol movement to the regulatory pool in the endoplasmic reticulum.	25-hydroxycholesterol	11-32	CCHC-type zinc finger nucleic acid binding protein	Homo sapiens	67-108
15250942-4	2004	Pulse-chase studies of 25HC-treated cells demonstrated enhanced degradation of tyrosinase, the rate-limiting enzyme of melanin synthesis, following endoplasmic reticulum (ER) and Golgi maturation.	25-hydroxycholesterol	23-27	tyrosinase	Mus musculus	79-89
15250942-5	2004	Protein levels of GS28, a member of an ER/cis-Golgi SNARE protein complex, were also diminished in 25HC-treated melanocytes, however levels of the ER chaperone calnexin and the cis-Golgi matrix protein GM130 were unaffected.	25-hydroxycholesterol	99-103	golgi SNAP receptor complex member 1	Mus musculus	18-22
15250942-5	2004	Protein levels of GS28, a member of an ER/cis-Golgi SNARE protein complex, were also diminished in 25HC-treated melanocytes, however levels of the ER chaperone calnexin and the cis-Golgi matrix protein GM130 were unaffected.	25-hydroxycholesterol	99-103	golgi autoantigen, golgin subfamily a, 2	Mus musculus	202-207
15250942-6	2004	Effects of 25HC on tyrosinase were completely reversed by 4 alpha-allylcholestan-3 alpha-ol, a sterol identified by its ability to reverse effects of 25HC on cholesterol homeostasis.	25-hydroxycholesterol	11-15	tyrosinase	Mus musculus	19-29
15250942-6	2004	Effects of 25HC on tyrosinase were completely reversed by 4 alpha-allylcholestan-3 alpha-ol, a sterol identified by its ability to reverse effects of 25HC on cholesterol homeostasis.	25-hydroxycholesterol	150-154	tyrosinase	Mus musculus	19-29
15250942-7	2004	Finally, the addition of 25HC to lipid deficient serum inhibited correct processing of tyrosinase.	25-hydroxycholesterol	25-29	tyrosinase	Mus musculus	87-97
14704295-4	2004	Incubation of HepG2 cells in serum-free medium supplemented with 25-hydroxycholesterol (25OH, 5 mg/L) for 24 h decreased LDLr protein and mRNA by 50-70% (P&lt;0.05) and mature SREBP-1 by 20-40% (P&lt;0.05).	25-hydroxycholesterol	65-86	low density lipoprotein receptor	Homo sapiens	121-125
14627708-3	2004	Apomine resembles sterols such as 25-hydroxycholesterol in its ability to potently accelerate the rate of HMGR degradation by the ubiquitin-proteasome pathway, a process that depends on the transmembrane domain of the enzyme.	25-hydroxycholesterol	34-55	3-hydroxy-3-methylglutaryl-CoA reductase	Rattus norvegicus	106-110
15084352-7	2004	In addition, induction of FPP synthase gene expression was abolished in the presence of 25-hydroxycholesterol (25-OH Chol).	25-hydroxycholesterol	88-109	farnesyl diphosphate synthetase	Mus musculus	26-38
14559920-4	2004	In this study, we demonstrate that, in the murine macrophage-like cell line P388D1, oxysterols (25-hydroxycholesterol and 7-ketocholesterol) induced the degradation of the prosurvival protein kinase AKT (protein kinase B).	25-hydroxycholesterol	96-117	thymoma viral proto-oncogene 1	Mus musculus	199-202
14559920-7	2004	Somewhat surprisingly, small interfering RNA knockdown of BAX resulted in a complete block of the induction of apoptosis by 25-hydroxycholesterol.	25-hydroxycholesterol	124-145	BCL2-associated X protein	Mus musculus	58-61
14709622-2	2004	Treatment of rat hepatocyte cultures with either 25-hydroxycholesterol or 24(S),25-epoxycholesterol (10(-5) to 5 x 10(-5) M) produced concentration-dependent elevations in CYP3A mRNA and immunoreactive protein levels but did not increase the amounts of CYP1A1, CYP2B, or CYP4A gene products.	25-hydroxycholesterol	49-70	cytochrome P450, family 3, subfamily a, polypeptide 62	Rattus norvegicus	172-177
14709622-2	2004	Treatment of rat hepatocyte cultures with either 25-hydroxycholesterol or 24(S),25-epoxycholesterol (10(-5) to 5 x 10(-5) M) produced concentration-dependent elevations in CYP3A mRNA and immunoreactive protein levels but did not increase the amounts of CYP1A1, CYP2B, or CYP4A gene products.	25-hydroxycholesterol	49-70	cytochrome P450, family 1, subfamily a, polypeptide 1	Rattus norvegicus	253-259
14704295-4	2004	Incubation of HepG2 cells in serum-free medium supplemented with 25-hydroxycholesterol (25OH, 5 mg/L) for 24 h decreased LDLr protein and mRNA by 50-70% (P&lt;0.05) and mature SREBP-1 by 20-40% (P&lt;0.05).	25-hydroxycholesterol	65-86	sterol regulatory element binding transcription factor 1	Homo sapiens	176-183
14704295-4	2004	Incubation of HepG2 cells in serum-free medium supplemented with 25-hydroxycholesterol (25OH, 5 mg/L) for 24 h decreased LDLr protein and mRNA by 50-70% (P&lt;0.05) and mature SREBP-1 by 20-40% (P&lt;0.05).	25-hydroxycholesterol	88-92	low density lipoprotein receptor	Homo sapiens	121-125
14704295-4	2004	Incubation of HepG2 cells in serum-free medium supplemented with 25-hydroxycholesterol (25OH, 5 mg/L) for 24 h decreased LDLr protein and mRNA by 50-70% (P&lt;0.05) and mature SREBP-1 by 20-40% (P&lt;0.05).	25-hydroxycholesterol	88-92	sterol regulatory element binding transcription factor 1	Homo sapiens	176-183
14644170-9	2003	Similarly, the collapse of vimentin intermediate filament network and the formation of lamellipodia, induced by 25-hydroxycholesterol, is inhibited by overexpressing dominant negatives forms of Rac1.	25-hydroxycholesterol	112-133	vimentin	Homo sapiens	27-35
14644170-9	2003	Similarly, the collapse of vimentin intermediate filament network and the formation of lamellipodia, induced by 25-hydroxycholesterol, is inhibited by overexpressing dominant negatives forms of Rac1.	25-hydroxycholesterol	112-133	Rac family small GTPase 1	Homo sapiens	194-198
12876624-4	2003	Treatment of HepG2 cells with 25-hydroxycholesterol lowered fibrinogen Aalpha, Bbeta and gamma mRNA levels and inhibited fibrinogen synthesis and secretion but had no effect on alpha1 -antitrypsin which, like fibrinogen, is an acute-phase protein.	25-hydroxycholesterol	30-51	fibrinogen beta chain	Homo sapiens	60-70
14612204-0	2003	Hypoxia increases 25-hydroxycholesterol-induced interleukin-8 protein secretion in human macrophages.	25-hydroxycholesterol	18-39	C-X-C motif chemokine ligand 8	Homo sapiens	48-61
14612204-4	2003	Hypoxia enhances 25-hydroxycholesterol (25-OH-chol)-induced IL-8 secretion in human monocyte-derived macrophages.	25-hydroxycholesterol	17-38	C-X-C motif chemokine ligand 8	Homo sapiens	60-64
14644170-5	2003	Cells treated with 25-hydroxycholesterol showed a collapse of vimentin filaments towards the nucleus and formation of lamellipodia.	25-hydroxycholesterol	19-40	vimentin	Homo sapiens	62-70
12876624-4	2003	Treatment of HepG2 cells with 25-hydroxycholesterol lowered fibrinogen Aalpha, Bbeta and gamma mRNA levels and inhibited fibrinogen synthesis and secretion but had no effect on alpha1 -antitrypsin which, like fibrinogen, is an acute-phase protein.	25-hydroxycholesterol	30-51	fibrinogen beta chain	Homo sapiens	121-131
12876624-4	2003	Treatment of HepG2 cells with 25-hydroxycholesterol lowered fibrinogen Aalpha, Bbeta and gamma mRNA levels and inhibited fibrinogen synthesis and secretion but had no effect on alpha1 -antitrypsin which, like fibrinogen, is an acute-phase protein.	25-hydroxycholesterol	30-51	fibrinogen beta chain	Homo sapiens	121-131
12916318-6	2003	To examine TCDD and PCDDs/PCDFs mixture action on cytochrome P450 side change cleavage enzyme (P450 scc) and 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) activity the placental cells were cultured either in basal conditions or with the addition of 25-hydroxycholesterol (25-OH) or pregnenolone (P5).	25-hydroxycholesterol	252-273	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1	Homo sapiens	146-156
12595494-7	2003	A natural ligand of LXRalpha, 25-hydroxycholesterol (25-OHC), had similar effects; when used together with PPAR agonists, an additive effect was observed, indicating co-operation between PPAR and LXRalpha in regulating ABCA1 gene expression.	25-hydroxycholesterol	30-51	nuclear receptor subfamily 1 group H member 3	Homo sapiens	20-28
12595494-7	2003	A natural ligand of LXRalpha, 25-hydroxycholesterol (25-OHC), had similar effects; when used together with PPAR agonists, an additive effect was observed, indicating co-operation between PPAR and LXRalpha in regulating ABCA1 gene expression.	25-hydroxycholesterol	53-59	nuclear receptor subfamily 1 group H member 3	Homo sapiens	20-28
12191470-5	2002	25-hydroxycholesterol, a potent regulator of SCAP in vivo, fails to change SCAP"s conformation in vitro, suggesting that oxysterols act in intact cells by translocating cholesterol from plasma membrane to ER.	25-hydroxycholesterol	0-21	SREBF chaperone	Homo sapiens	45-49
12401528-7	2003	Addition of 25-hydroxycholesterol (25-HC), an inhibitor of the expression of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase and synthetase to cells in LPDS, further decreased GTPase activity in a dose-dependent manner.	25-hydroxycholesterol	12-33	3-hydroxy-3-methylglutaryl-CoA reductase	Rattus norvegicus	77-134
12401528-7	2003	Addition of 25-hydroxycholesterol (25-HC), an inhibitor of the expression of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase and synthetase to cells in LPDS, further decreased GTPase activity in a dose-dependent manner.	25-hydroxycholesterol	35-40	3-hydroxy-3-methylglutaryl-CoA reductase	Rattus norvegicus	77-134
12149270-5	2002	Both deoxycholic acid and lithocholic acid as well as CDCA, but not ursodeoxycholic acid, increase the mRNA level for the LDL receptor, even when Hep G2 cells are cultured with 25-hydroxycholesterol, a potent suppressor of gene expression for the LDL receptor.	25-hydroxycholesterol	177-198	low density lipoprotein receptor	Homo sapiens	122-134
12149270-5	2002	Both deoxycholic acid and lithocholic acid as well as CDCA, but not ursodeoxycholic acid, increase the mRNA level for the LDL receptor, even when Hep G2 cells are cultured with 25-hydroxycholesterol, a potent suppressor of gene expression for the LDL receptor.	25-hydroxycholesterol	177-198	low density lipoprotein receptor	Homo sapiens	247-259
12085989-7	2002	In cultured HepG2 or murine macrophage RAW264.7 cells, 3 days treatment with either 25-hydroxycholesterol or 7-ketocholesterol induced iNOS mRNA expression, as determined by RT-PCR.	25-hydroxycholesterol	84-105	nitric oxide synthase 2, inducible	Mus musculus	135-139
11967256-4	2002	In transfected hepatoma cells, 25-hydroxycholesterol increased murine Cyp7a1 gene promoter activity, whereas the human CYP7A1 gene promoter was unresponsive.	25-hydroxycholesterol	31-52	cytochrome P450, family 7, subfamily a, polypeptide 1	Mus musculus	70-76
11857482-9	2002	The inhibitory actions of TNFalpha on T production were greatly reduced by treatment of testis with 25-hydroxycholesterol (1 and 10 microg/ml), pregnenolone (50 and 100 ng/ml), and 17 alpha-hydroxypregesterone (50 ng/ml).	25-hydroxycholesterol	100-121	tumor necrosis factor	Mus musculus	26-34
11846416-5	2002	The effects of SQ1 and its reversal by 25-hydroxycholesterol and the effects of bile acids were reproduced in reporter gene assays with a phenobarbital-responsive enhancer unit of CYP2H1.	25-hydroxycholesterol	39-60	cytochrome P450 2H1	Gallus gallus	180-186
11590225-6	2001	In the current study, we demonstrate that activation of cPLA2 does occur in both macrophages and fibroblasts treated with 25-OHC or oxLDL.	25-hydroxycholesterol	122-128	phospholipase A2 group IVA	Homo sapiens	56-61
11583711-5	2001	Treatment of human peripheral blood mononuclear leukocytes with oxidized LDL or the combination of cholesterol and 25-hydroxycholesterol caused activation of p38 alpha as determined by the ability of immunoprecipitated p38 to phosphorylate an ATF-2 fusion protein, a surrogate substrate of p38 alpha.	25-hydroxycholesterol	115-136	mitogen-activated protein kinase 14	Homo sapiens	158-167
11583711-5	2001	Treatment of human peripheral blood mononuclear leukocytes with oxidized LDL or the combination of cholesterol and 25-hydroxycholesterol caused activation of p38 alpha as determined by the ability of immunoprecipitated p38 to phosphorylate an ATF-2 fusion protein, a surrogate substrate of p38 alpha.	25-hydroxycholesterol	115-136	mitogen-activated protein kinase 14	Homo sapiens	158-161
11583711-5	2001	Treatment of human peripheral blood mononuclear leukocytes with oxidized LDL or the combination of cholesterol and 25-hydroxycholesterol caused activation of p38 alpha as determined by the ability of immunoprecipitated p38 to phosphorylate an ATF-2 fusion protein, a surrogate substrate of p38 alpha.	25-hydroxycholesterol	115-136	activating transcription factor 2	Homo sapiens	243-248
11583711-5	2001	Treatment of human peripheral blood mononuclear leukocytes with oxidized LDL or the combination of cholesterol and 25-hydroxycholesterol caused activation of p38 alpha as determined by the ability of immunoprecipitated p38 to phosphorylate an ATF-2 fusion protein, a surrogate substrate of p38 alpha.	25-hydroxycholesterol	115-136	mitogen-activated protein kinase 14	Homo sapiens	290-299
11707442-7	2002	Furthermore, the inhibition of cholesterol synthesis with simvastatin resulted in a 3-4-fold increase in both SR-BI mRNA and protein levels, whereas conversely, addition of 25-hydroxycholesterol suppressed SR-BI levels by approximately 50%.	25-hydroxycholesterol	173-194	scavenger receptor class B member 1	Homo sapiens	206-211
11851725-6	2002	RESULTS: A significant, dose-dependent increase in the secretion of IL-1beta was given by 25-hydroxycholesterol without the addition of lipopolysaccharide (LPS).	25-hydroxycholesterol	90-111	interleukin 1 beta	Homo sapiens	68-76
11851725-8	2002	A transient increase in IL-1beta mRNA expression was found in macrophages incubated with 25-hydroxycholesterol compared with untreated controls.	25-hydroxycholesterol	89-110	interleukin 1 beta	Homo sapiens	24-32
11851725-9	2002	In addition, 25-hydroxycholesterol dramatically increased the IL-1beta secretion induced by LPS.	25-hydroxycholesterol	13-34	interleukin 1 beta	Homo sapiens	62-70
11851725-10	2002	At a concentration of 5 microg mL(-1) of 25-hydroxycholesterol the LPS-induced IL-1beta secretion was increased by about 25-fold.	25-hydroxycholesterol	41-62	interleukin 1 beta	Homo sapiens	79-87
11726279-3	2001	We found that 1:1 stoichiometric binding of oxidized adrenodoxin to oxidized cytochrome P450scc complexed with cholesterol or 25-hydroxycholesterol caused shifts of the high-spin EPR signals of the heme moiety at 5 K. Such shifts were not observed for the low-spin EPR signals.	25-hydroxycholesterol	126-147	cytochrome P450 family 11 subfamily A member 1	Homo sapiens	77-95
11590225-7	2001	Activation is evidenced by 25-OHC-induced relocalization of cPLA2 to the nuclear envelope and arachidonic acid release.	25-hydroxycholesterol	27-33	phospholipase A2 group IVA	Homo sapiens	60-65
11590225-8	2001	Loss of cPLA2 activity, either through genetic knockout in mice, or by treatment with a cPLA2 inhibitor, results in an attenuation of arachidonic acid release as well as of the apoptotic response to oxLDL in peritoneal macrophages or to 25-OHC in cultured fibroblast and macrophage cell lines.	25-hydroxycholesterol	237-243	phospholipase A2, group IVA (cytosolic, calcium-dependent)	Mus musculus	8-13
11590225-8	2001	Loss of cPLA2 activity, either through genetic knockout in mice, or by treatment with a cPLA2 inhibitor, results in an attenuation of arachidonic acid release as well as of the apoptotic response to oxLDL in peritoneal macrophages or to 25-OHC in cultured fibroblast and macrophage cell lines.	25-hydroxycholesterol	237-243	phospholipase A2, group IVA (cytosolic, calcium-dependent)	Mus musculus	88-93
11500175-0	2001	25-hydroxycholesterol induces lipopolysaccharide-tolerance and decreases a lipopolysaccharide-induced TNF-alpha secretion in macrophages.	25-hydroxycholesterol	0-21	tumor necrosis factor	Homo sapiens	102-111
11500175-5	2001	The most pronounced effect was obtained with 25-hydroxycholesterol, where the TNF-alpha secretion was reduced to 8%.	25-hydroxycholesterol	45-66	tumor necrosis factor	Homo sapiens	78-87
11207193-9	2001	These results demonstrate that 1) testicular macrophages have mRNA for cholesterol 25-hydroxylase and can convert cholesterol into 25-hydroxycholesterol, 2) macrophage-conditioned medium is not capable of auto-oxidation of cholesterol, 3) Leydig cells are highly resistant to the cytotoxic influences of 25-hydroxycholesterol, and 4) long-term treatment with high doses of 25-hydroxycholesterol results in loss of LH responsiveness.	25-hydroxycholesterol	304-325	cholesterol 25-hydroxylase	Rattus norvegicus	71-97
11284740-0	2001	cyp7b1 catalyses the 7alpha-hydroxylation of dehydroepiandrosterone and 25-hydroxycholesterol in rat prostate.	25-hydroxycholesterol	72-93	cytochrome P450 family 7 subfamily B member 1	Rattus norvegicus	0-6
11284740-5	2001	We recently described a novel cytochrome P450 enzyme, cyp7b1, strongly expressed in the hippocampus of rodent brain, which catalyses the metabolism of DHEA, pregnenolone and 25-hydroxycholesterol to 7alpha-hydroxy products.	25-hydroxycholesterol	174-195	cytochrome P450 family 7 subfamily B member 1	Rattus norvegicus	54-60
11590226-11	2001	In contrast, if 25-hydroxycholesterol was substituted for cholesterol, both the precursor and mature forms of SREBP-2 were decreased.	25-hydroxycholesterol	16-37	sterol regulatory element binding transcription factor 2	Homo sapiens	110-117
11279184-10	2001	Purification of ORP1 and ORP2 for ligand binding studies demonstrated ORP1 and ORP2 did not bind 25-hydroxycholesterol but instead bound phospholipids with both proteins exhibiting strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate.	25-hydroxycholesterol	97-118	peroxiredoxin HYR1	Saccharomyces cerevisiae S288C	16-20
10591667-5	1999	25-OHC induces SMCs to change morphologically, increase PGHS-2, and increase eicosanoid release.	25-hydroxycholesterol	0-6	prostaglandin-endoperoxide synthase 2	Homo sapiens	56-62
11402689-7	2001	A stimulatory effect of NA on cytochrome P-450scc activity (P &lt; 0.05) was demonstrated using 25-hydroxycholesterol as substrate.	25-hydroxycholesterol	96-117	cholesterol side-chain cleavage enzyme, mitochondrial	Bos taurus	30-49
11095949-0	2000	25-Hydroxycholesterol activates a cytochrome c release-mediated caspase cascade.	25-hydroxycholesterol	0-21	cytochrome c	Cricetulus griseus	34-46
11095949-4	2000	The addition of a competitive caspase-3 inhibitor, Ac-DEVD-CHO, prevented 25-OHC-induced apoptotic cell death.	25-hydroxycholesterol	74-80	caspase-3	Cricetulus griseus	30-39
11095949-5	2000	Furthermore, immunoblot analysis showed that 25-OHC treatment induced the degradation of poly(ADP-ribose) polymerase (PARP)-a substrate for caspase 3 and a key enzyme involved in genome surveillance and DNA repair.	25-hydroxycholesterol	45-51	poly [ADP-ribose] polymerase 1	Cricetulus griseus	89-116
11095949-5	2000	Furthermore, immunoblot analysis showed that 25-OHC treatment induced the degradation of poly(ADP-ribose) polymerase (PARP)-a substrate for caspase 3 and a key enzyme involved in genome surveillance and DNA repair.	25-hydroxycholesterol	45-51	poly [ADP-ribose] polymerase 1	Cricetulus griseus	118-122
11095949-5	2000	Furthermore, immunoblot analysis showed that 25-OHC treatment induced the degradation of poly(ADP-ribose) polymerase (PARP)-a substrate for caspase 3 and a key enzyme involved in genome surveillance and DNA repair.	25-hydroxycholesterol	45-51	caspase-3	Cricetulus griseus	140-149
10882719-5	2000	Surprisingly, human cholesterol 7 alpha-hydroxylase, CYP7A, expressed as a recombinant in Escherichia coli and COS cells, was active toward 20(S)-hydroxycholesterol, 25-hydroxycholesterol, and 27-hydroxycholesterol.	25-hydroxycholesterol	166-187	cytochrome P450 family 7 subfamily A member 1	Homo sapiens	20-51
10751394-10	2000	Finally, we showed that the lysosomal cholesterol pool in NP-C cells was substantially and preferentially reduced by incubating cells with the oxysterols, 25-hydroxycholesterol and 7-ketocholesterol; these findings suggest a new pharmacological approach to the treatment of NP-C disease.	25-hydroxycholesterol	155-176	NPC intracellular cholesterol transporter 1	Homo sapiens	58-62
10702300-12	2000	Treatment of CHO or THP-1 (macrophage) cells with the calcium channel blocker nifedipine prevented 25-hydroxycholesterol induction of apoptosis.	25-hydroxycholesterol	99-120	GLI family zinc finger 2	Homo sapiens	20-25
11693580-0	2001	Control of apolipoprotein E secretion by 25-hydroxycholesterol and proinflammatory cytokines in the human astrocytoma cell line CCF-STTG1.	25-hydroxycholesterol	41-62	apolipoprotein E	Homo sapiens	11-27
11693580-8	2001	A time-dependent stimulation of apo E secretion by 25-hydroxycholesterol was observed.	25-hydroxycholesterol	51-72	apolipoprotein E	Homo sapiens	32-37
11693580-10	2001	In the presence of 25-hydroxycholesterol, TNF-alpha reduced apo E secretion by 80%, while interleukins (IL) IL-1beta, IL-6, and IL-2 had no significant effects.	25-hydroxycholesterol	19-40	tumor necrosis factor	Homo sapiens	42-51
11693580-10	2001	In the presence of 25-hydroxycholesterol, TNF-alpha reduced apo E secretion by 80%, while interleukins (IL) IL-1beta, IL-6, and IL-2 had no significant effects.	25-hydroxycholesterol	19-40	apolipoprotein E	Homo sapiens	60-65
10498032-1	1999	4alpha-(2-Propenyl)-5alpha-cholest-24-en-3alpha-ol (3) was shown recently in a Chinese hamster ovary (CHO) cell-based low-density lipoprotein receptor/luciferase (LDLR/Luc) assay to be a potent transcriptional activator of the LDL receptor promoter in the presence of 25-hydroxycholesterol.	25-hydroxycholesterol	268-289	low-density lipoprotein receptor	Cricetulus griseus	118-150
10478848-9	1999	The putative SF-1 ligand, 25-hydroxycholesterol, has no effects on these bindings.	25-hydroxycholesterol	26-47	nuclear receptor subfamily 5 group A member 1	Homo sapiens	13-17
10427141-6	1999	These findings suggest that AH136B cells in the sub-G1 phase can not recover tumorigenicity, and that the administration of a 3-hydroxy-3-methylglutaryl CoA reductase inhibitor, such as 25-OH, and ATRA may be effective in treating patients with hepatoma.	25-hydroxycholesterol	186-191	3-hydroxy-3-methylglutaryl-CoA reductase	Rattus norvegicus	126-166
10521709-3	1999	25-Hydroxycholesterol, which inhibited non-esterified cholesterol synthesis but increased the synthesis of cholesteryl ester, showed differential effects on the metabolism of apoB-48 and apoB-100.	25-hydroxycholesterol	0-21	apolipoprotein B	Rattus norvegicus	175-182
10521709-3	1999	25-Hydroxycholesterol, which inhibited non-esterified cholesterol synthesis but increased the synthesis of cholesteryl ester, showed differential effects on the metabolism of apoB-48 and apoB-100.	25-hydroxycholesterol	0-21	apolipoprotein B	Rattus norvegicus	187-195
10521709-5	1999	The post-translational degradation of apoB-48, but not of apoB-100, was enhanced by 25-hydroxycholesterol.	25-hydroxycholesterol	84-105	apolipoprotein B	Rattus norvegicus	38-45
10521709-7	1999	The inhibitory effect of lovastatin alone on the net synthesis of apoB-48 and apoB-100 was reversed by the simultaneous presence of 25-hydroxycholesterol, suggesting a role for newly synthesised cholesteryl ester.	25-hydroxycholesterol	132-153	apolipoprotein B	Rattus norvegicus	78-86
10521709-9	1999	In the presence of lovastatin, restoration of the net synthesis of apoB by 25-hydroxycholesterol was not accompanied by an increased VLDL output of apoB-48 and apoB-100.	25-hydroxycholesterol	75-96	apolipoprotein B	Rattus norvegicus	67-71
9500571-5	1998	Similar results were obtained in cells in which ACAT activity was induced by preincubation either with 25-hydroxycholesterol and mevalonic acid or with CEase and bile salt mixed-micelles containing 100 micromol/L cholesterol.	25-hydroxycholesterol	103-124	carboxylesterase 1	Homo sapiens	48-52
10202850-2	1999	NTE-122 markably inhibited [3H]oleate incorporation into cholesteryl esters in HepG2 cells incubated with 5 microg/ml 25-hydroxycholesterol as a stimulus for ACAT (IC50=6.0 nM).	25-hydroxycholesterol	118-139	patatin like phospholipase domain containing 6	Homo sapiens	0-3
10202850-2	1999	NTE-122 markably inhibited [3H]oleate incorporation into cholesteryl esters in HepG2 cells incubated with 5 microg/ml 25-hydroxycholesterol as a stimulus for ACAT (IC50=6.0 nM).	25-hydroxycholesterol	118-139	sterol O-acyltransferase 1	Homo sapiens	158-162
10202850-4	1999	The stimulation of ACAT by 25-hydroxycholesterol caused significant increases in the secretion of radiolabeled cholesteryl esters, radiolabeled triglycerides and apoB mass.	25-hydroxycholesterol	27-48	sterol O-acyltransferase 1	Homo sapiens	19-23
10202850-4	1999	The stimulation of ACAT by 25-hydroxycholesterol caused significant increases in the secretion of radiolabeled cholesteryl esters, radiolabeled triglycerides and apoB mass.	25-hydroxycholesterol	27-48	apolipoprotein B	Homo sapiens	162-166
9918530-6	1999	Evaluation of the effect of oxysterol components of OxLDL on TIMP-1 production revealed that 25-hydroxycholesterol (1 microg/mL) was the most potent inhibitor ( approximately 30% of control).	25-hydroxycholesterol	93-114	TIMP metallopeptidase inhibitor 1	Homo sapiens	61-67
9880570-3	1999	Oxysterols (25-hydroxycholesterol and 7-ketocholesterol) reversed the inhibition of apoB degradation caused by 7alpha-hydroxylase.	25-hydroxycholesterol	12-33	apolipoprotein B	Homo sapiens	84-88
10333389-10	1999	The transcriptional regulator 25-hydroxycholesterol suppresses both HMGR and LDLR activities, while the post-transcriptional regulatory lanosterol analogs exhibit a more desirable profile, lowering HMGR levels without suppressing LDLR expression, and in some cases actually enhancing cellular LDL metabolism.	25-hydroxycholesterol	30-51	high mobility group AT-hook 1	Homo sapiens	68-72
10333389-10	1999	The transcriptional regulator 25-hydroxycholesterol suppresses both HMGR and LDLR activities, while the post-transcriptional regulatory lanosterol analogs exhibit a more desirable profile, lowering HMGR levels without suppressing LDLR expression, and in some cases actually enhancing cellular LDL metabolism.	25-hydroxycholesterol	30-51	low density lipoprotein receptor	Homo sapiens	77-81
9869656-11	1999	Results from overexpressing cells show that OSBP potentiates the stimulatory effects of 25-hydroxycholesterol on sphingomyelin synthesis.	25-hydroxycholesterol	88-109	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	44-48
9869656-12	1999	25-Hydroxycholesterol promotes translocation of OSBP to the Golgi apparatus where it appears to stimulate conversion of ceramide to sphingomyelin.	25-hydroxycholesterol	0-21	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	48-52
9806908-0	1998	Cholesterol regulates oxysterol binding protein (OSBP) phosphorylation and Golgi localization in Chinese hamster ovary cells: correlation with stimulation of sphingomyelin synthesis by 25-hydroxycholesterol.	25-hydroxycholesterol	185-206	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	49-53
9694903-4	1998	Our data indicate that C18 fatty acids can potentiate the biological activities of a typical, regulatory sterol: 25-hydroxycholesterol.	25-hydroxycholesterol	113-134	Bardet-Biedl syndrome 9	Homo sapiens	23-26
9694903-5	1998	Inhibition of C18 fatty acid synthesis in cells cultured in serum-free medium renders them resistant to killing by 25-hydroxycholesterol.	25-hydroxycholesterol	115-136	Bardet-Biedl syndrome 9	Homo sapiens	14-17
9694903-6	1998	Repression of expression of reporter constructs driven by promoters bearing SRE-1 element(s) by 25-hydroxycholesterol is increased by C18 fatty acid supplementation.	25-hydroxycholesterol	96-117	Bardet-Biedl syndrome 9	Homo sapiens	134-137
9694903-7	1998	C18 fatty acids also increase the inhibitory effect of 25-hydroxycholesterol on proteolytic maturation and nuclear localization of SREBPs.	25-hydroxycholesterol	55-76	Bardet-Biedl syndrome 9	Homo sapiens	0-3
9694903-8	1998	Furthermore, we also show that C18 fatty acid supplementation can enhance the inhibitory effect of 25-hydroxycholesterol on sterol and fatty acid biosynthesis.	25-hydroxycholesterol	99-120	Bardet-Biedl syndrome 9	Homo sapiens	31-34
10400383-7	1999	In summary, the observed LDL receptor promoter activity of 17 is related to its ability to prevent 25-hydroxycholesterol from exerting the repressing effect via an undetermined mechanism and, in part, to inhibit the cholesterol biosynthesis.	25-hydroxycholesterol	99-120	low-density lipoprotein receptor	Cricetulus griseus	25-37
10202850-5	1999	NTE-122 pronouncedly inhibited the secretion of radiolabeled cholesteryl esters in proportion to the inhibition of cellular cholesterol esterification, and it significantly reduced the secretion of radiolabeled triglycerides and apoB mass in HepG2 cells incubated with 25-hydroxycholesterol.	25-hydroxycholesterol	269-290	patatin like phospholipase domain containing 6	Homo sapiens	0-3
9869656-0	1999	Chinese hamster ovary cells overexpressing the oxysterol binding protein (OSBP) display enhanced synthesis of sphingomyelin in response to 25-hydroxycholesterol.	25-hydroxycholesterol	139-160	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	47-72
9869656-0	1999	Chinese hamster ovary cells overexpressing the oxysterol binding protein (OSBP) display enhanced synthesis of sphingomyelin in response to 25-hydroxycholesterol.	25-hydroxycholesterol	139-160	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	74-78
9869656-3	1999	The role of oxysterol binding protein (OSBP), a high affinity receptor for 25-hydroxycholesterol, in activation of SM synthesis was assessed by overexpression in CHO-K1 cells.	25-hydroxycholesterol	75-96	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	12-37
9869656-3	1999	The role of oxysterol binding protein (OSBP), a high affinity receptor for 25-hydroxycholesterol, in activation of SM synthesis was assessed by overexpression in CHO-K1 cells.	25-hydroxycholesterol	75-96	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	39-43
9869656-4	1999	When compared to mock transfected controls, three CHO-K1 clones overexpressing OSBP by 10- to 15-fold displayed a 2- to 3-fold enhancement of [3H]serine incorporation into sphingomyelin when treated with 25-hydroxycholesterol.	25-hydroxycholesterol	204-225	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	79-83
9869656-5	1999	Closer examination of one of these clones (CHO-OSBP cells) revealed a &gt;8.5-fold stimulation of sphingomyelin synthesis after a 6-h treatment with 25-hydroxycholesterol compared to 3.5-fold in controls, slightly higher basal levels of sphingomyelin synthesis, and a more rapid response to 25-hydroxycholesterol.	25-hydroxycholesterol	149-170	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	47-51
9869656-5	1999	Closer examination of one of these clones (CHO-OSBP cells) revealed a &gt;8.5-fold stimulation of sphingomyelin synthesis after a 6-h treatment with 25-hydroxycholesterol compared to 3.5-fold in controls, slightly higher basal levels of sphingomyelin synthesis, and a more rapid response to 25-hydroxycholesterol.	25-hydroxycholesterol	291-312	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	47-51
9789003-3	1998	Sterols such as 25-hydroxycholesterol inactivate SCAP, suppressing SREBP proteolysis and turning off cholesterol synthesis.	25-hydroxycholesterol	16-37	sterol regulatory element-binding protein cleavage-activating protein	Cricetulus griseus	49-53
9789003-4	1998	We here report the isolation of Chinese hamster ovary cells with a point mutation in SCAP (Y298C) that renders the protein resistant to inhibition by 25-hydroxycholesterol.	25-hydroxycholesterol	150-171	sterol regulatory element-binding protein cleavage-activating protein	Cricetulus griseus	85-89
9789003-6	1998	Cells that express SCAP(Y298C) continued to process SREBPs in the presence of 25-hydroxycholesterol and hence they resisted killing by this sterol.	25-hydroxycholesterol	78-99	sterol regulatory element-binding protein cleavage-activating protein	Cricetulus griseus	19-23
9789003-7	1998	In wild-type Chinese hamster ovary cells the N-linked carbohydrate chains of SCAP were mostly in the endoglycosidase H-sensitive form when cells were grown in medium containing 25-hydroxycholesterol.	25-hydroxycholesterol	177-198	sterol regulatory element-binding protein cleavage-activating protein	Cricetulus griseus	77-81
9422368-8	1998	ApoD was found to be a secreted protein from cultured normal astrocytes and treatment with the oxysterol, 25-hydroxycholesterol, markedly stimulated apoD release (by five- to 10-fold).	25-hydroxycholesterol	106-127	apolipoprotein D	Mus musculus	0-4
9487139-1	1998	Oxysterol binding protein (OSBP), a high affinity receptor for 25-hydroxycholesterol that localizes to a Golgi/vesicular compartment, migrated on SDS-PAGE as a doublet of 96 and 101 kDa.	25-hydroxycholesterol	63-84	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	0-25
9487139-1	1998	Oxysterol binding protein (OSBP), a high affinity receptor for 25-hydroxycholesterol that localizes to a Golgi/vesicular compartment, migrated on SDS-PAGE as a doublet of 96 and 101 kDa.	25-hydroxycholesterol	63-84	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	27-31
9422368-8	1998	ApoD was found to be a secreted protein from cultured normal astrocytes and treatment with the oxysterol, 25-hydroxycholesterol, markedly stimulated apoD release (by five- to 10-fold).	25-hydroxycholesterol	106-127	apolipoprotein D	Mus musculus	149-153
8662991-5	1996	These data provide further insight into the interplay between ACAT activation and inhibition of SREBP cleavage by 25-hydroxycholesterol, and they indicate that these two processes can be disrupted independently by mutation.	25-hydroxycholesterol	114-135	sterol O-acyltransferase 1	Cricetulus griseus	62-66
9351364-12	1997	In vitro incubations of peritoneal macrophages with 125I-LDL indicated that the LDLR of these cells could be downregulated by 25-hydroxycholesterol.	25-hydroxycholesterol	126-147	low density lipoprotein receptor	Mus musculus	80-84
9374127-7	1997	Furthermore, activation of the ICE-like protease caspase 3 (CPP32) was observed in cells treated with 25-hydroxycholesterol.	25-hydroxycholesterol	102-123	caspase 3	Homo sapiens	49-58
9374127-7	1997	Furthermore, activation of the ICE-like protease caspase 3 (CPP32) was observed in cells treated with 25-hydroxycholesterol.	25-hydroxycholesterol	102-123	caspase 3	Homo sapiens	60-65
9337870-3	1997	Compared with mock-transfected controls, several cell lines overexpressing wild-type OSBP (CHO-OSBP) displayed a 50% decrease in cholesteryl ester synthesis when cultured in medium with delipidated serum, 25-hydroxycholesterol or low-density lipoprotein (LDL).	25-hydroxycholesterol	205-226	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	85-89
9337870-3	1997	Compared with mock-transfected controls, several cell lines overexpressing wild-type OSBP (CHO-OSBP) displayed a 50% decrease in cholesteryl ester synthesis when cultured in medium with delipidated serum, 25-hydroxycholesterol or low-density lipoprotein (LDL).	25-hydroxycholesterol	205-226	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	91-99
9235933-6	1997	The increased cellular content of mature SREBP1 and increased secretion of apoB100 were concomitantly reversed by 25-hydroxycholesterol, suggesting that the content of mature SREBP1, known to be decreased by 25-hydroxycholesterol, mediates the changes in the lipoprotein assembly and secretion pathway that are caused by 7alpha-hydroxylase.	25-hydroxycholesterol	114-135	sterol regulatory element binding transcription factor 1	Rattus norvegicus	41-47
9235933-6	1997	The increased cellular content of mature SREBP1 and increased secretion of apoB100 were concomitantly reversed by 25-hydroxycholesterol, suggesting that the content of mature SREBP1, known to be decreased by 25-hydroxycholesterol, mediates the changes in the lipoprotein assembly and secretion pathway that are caused by 7alpha-hydroxylase.	25-hydroxycholesterol	114-135	apolipoprotein B	Rattus norvegicus	75-82
9235933-6	1997	The increased cellular content of mature SREBP1 and increased secretion of apoB100 were concomitantly reversed by 25-hydroxycholesterol, suggesting that the content of mature SREBP1, known to be decreased by 25-hydroxycholesterol, mediates the changes in the lipoprotein assembly and secretion pathway that are caused by 7alpha-hydroxylase.	25-hydroxycholesterol	114-135	sterol regulatory element binding transcription factor 1	Rattus norvegicus	175-181
9247143-3	1997	Oxysterols (7-ketocholesterol, 25-hydroxycholesterol) induced nuclear condensation and oligonucleosomal DNA fragmentation, which were partially inhibited by Bcl-2 over-expression.	25-hydroxycholesterol	31-52	B cell leukemia/lymphoma 2	Mus musculus	157-162
9017510-1	1996	LY295427, (3 alpha,4 alpha,5 alpha)-4-(2-propenylcholestan-3-ol), acts through an unknown mechanism to derepress the transcription of the low density lipoprotein (LDL) receptor in the presence of 25-hydroxycholesterol (25-OH chol).	25-hydroxycholesterol	196-217	low-density lipoprotein receptor	Cricetulus griseus	138-176
8942999-7	1996	The D443N substitution is an activating mutation that increases the activity of SCAP and renders it resistant to inhibition by 25-hydroxycholesterol.	25-hydroxycholesterol	127-148	sterol regulatory element-binding protein cleavage-activating protein	Cricetulus griseus	80-84
8942999-11	1996	Nevertheless, the finding of the same nucleotide substitution at the same location in all three cell lines indicates that SCAP may be unique in its ability to stimulate SREBP cleavage, and residue 443 is a crucial determinant of the protein"s ability to be inhibited by 25-hydroxycholesterol.	25-hydroxycholesterol	270-291	sterol regulatory element-binding protein cleavage-activating protein	Cricetulus griseus	122-126
9351383-8	1997	In contrast, no significant effect on the level of mRNA for the LDL receptor was observed after incubation with cafestol, whereas 25-hydroxycholesterol reduced the mRNA level for the LDL receptor by 40% to 50% (P &lt; .05).	25-hydroxycholesterol	130-151	low density lipoprotein receptor	Homo sapiens	183-195
9337870-1	1997	Oxysterol-binding protein (OSBP) is a high-affinity receptor for a variety of oxysterols, such as 25-hydroxycholesterol, that down-regulate cholesterol synthesis and stimulate cholesterol esterification.	25-hydroxycholesterol	98-119	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	0-25
9337870-1	1997	Oxysterol-binding protein (OSBP) is a high-affinity receptor for a variety of oxysterols, such as 25-hydroxycholesterol, that down-regulate cholesterol synthesis and stimulate cholesterol esterification.	25-hydroxycholesterol	98-119	LOW QUALITY PROTEIN: oxysterol-binding protein 1	Cricetulus griseus	27-31
9084508-0	1997	25-Hydroxycholesterol enhances eicosanoid production in cultured bovine coronary artery endothelial cells by increasing prostaglandin G/H synthase-2.	25-hydroxycholesterol	0-21	prostaglandin-endoperoxide synthase 2	Bos taurus	120-148
9059514-1	1997	The metabolism of 25-hydroxycholesterol in different cell types was studied and the role of 7 alpha-hydroxylation for the effect of 25-hydroxycholesterol on the activity of HMG-CoA reductase was determined.	25-hydroxycholesterol	132-153	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	173-190
9059514-5	1997	The 7 alpha-hydroxylated metabolites of 25-hydroxycholesterol suppressed the activity of HMG-CoA reductase to a similar extent as 25-hydroxycholesterol in HDF but not in 90VA-VI cells, while the 7 alpha-hydroxylated metabolites of 27-hydroxycholesterol suppressed the activity of HMG-CoA reductase also in 90VA-VI cells.	25-hydroxycholesterol	40-61	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	89-106
9059514-5	1997	The 7 alpha-hydroxylated metabolites of 25-hydroxycholesterol suppressed the activity of HMG-CoA reductase to a similar extent as 25-hydroxycholesterol in HDF but not in 90VA-VI cells, while the 7 alpha-hydroxylated metabolites of 27-hydroxycholesterol suppressed the activity of HMG-CoA reductase also in 90VA-VI cells.	25-hydroxycholesterol	40-61	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	280-297
9059514-5	1997	The 7 alpha-hydroxylated metabolites of 25-hydroxycholesterol suppressed the activity of HMG-CoA reductase to a similar extent as 25-hydroxycholesterol in HDF but not in 90VA-VI cells, while the 7 alpha-hydroxylated metabolites of 27-hydroxycholesterol suppressed the activity of HMG-CoA reductase also in 90VA-VI cells.	25-hydroxycholesterol	130-151	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	89-106
9002660-2	1997	Human placental syncytiotrophoblasts were cultured for 48 h with estradiol, and P450scc activity was determined by the formation of progesterone from 25-hydroxycholesterol.	25-hydroxycholesterol	150-171	cytochrome P450 family 11 subfamily A member 1	Homo sapiens	80-87
9034205-8	1997	To determine whether the increase in transcriptional activity of the LDL-R gene was secondary to changes in the cholesterol regulatory pool we performed experiments in which the cell cholesterol content was modified by the addition of either 25-hydroxycholesterol and mevalonate or inhibitors of ACAT activity (SA-58035 and progesterone).	25-hydroxycholesterol	242-263	low density lipoprotein receptor	Homo sapiens	69-74
8831713-3	1996	We show that both 7-ketocholesterol and 25-hydroxycholesterol induced apoptosis, followed by a rapid decrease in bcl-2 protein in the cells.	25-hydroxycholesterol	40-61	BCL2 apoptosis regulator	Homo sapiens	113-118
8831713-4	1996	CPP32/Yamma inhibitor prevented apoptosis induced by 7-ketocholesterol and 25-hydroxycholesterol in VSMCs, whereas the exogenous cholesterol, which itself did not have any apoptotic activity, inhibited 25-hydroxycholesterol induced apoptosis but the 7-ketocholesterol-induced apoptosis was not affected.	25-hydroxycholesterol	75-96	caspase 3	Homo sapiens	0-5
8831713-4	1996	CPP32/Yamma inhibitor prevented apoptosis induced by 7-ketocholesterol and 25-hydroxycholesterol in VSMCs, whereas the exogenous cholesterol, which itself did not have any apoptotic activity, inhibited 25-hydroxycholesterol induced apoptosis but the 7-ketocholesterol-induced apoptosis was not affected.	25-hydroxycholesterol	202-223	caspase 3	Homo sapiens	0-5
8662991-3	1996	In the current studies we show that SRD-4 cells have three defects: 1) a point mutation in one allele at the ACAT locus that changes codon 265 from Ser to Leu, resulting in an inactive enzyme; 2) a silent allele at the other ACAT locus that does not produce detectable mRNA; and 3) a mutation, as yet undefined, that abolishes the ability of 25-hydroxycholesterol to inhibit the cleavage of both sterol regulatory element binding proteins (SREBP-1 and SREBP-2).	25-hydroxycholesterol	342-363	sterol O-acyltransferase 1	Cricetulus griseus	109-113
8652654-3	1996	When HepG2 cells were loaded with cholesterol and 25-hydroxycholesterol, both the whole-cell ACAT activity and the microsomal ACAT activity were increased by 85.1% and 41.3%.	25-hydroxycholesterol	50-71	carboxylesterase 1	Homo sapiens	93-97
8652654-3	1996	When HepG2 cells were loaded with cholesterol and 25-hydroxycholesterol, both the whole-cell ACAT activity and the microsomal ACAT activity were increased by 85.1% and 41.3%.	25-hydroxycholesterol	50-71	carboxylesterase 1	Homo sapiens	126-130
8615754-4	1996	When THP-1 monocytic cells were cultured in the presence of 7beta-hydroxycholesterol (7beta-OH) or 25-hydroxycholesterol (25-OH), prothrombinase, which reflects anionic phospholipid exposure and tissue factor (TF) procoagulant activities, increased in a time- and dose-dependent manner.	25-hydroxycholesterol	99-120	GLI family zinc finger 2	Homo sapiens	5-10
8619637-8	1996	In the case of CYP51, cholesterol deprivation led to a 2.6- to 3.8-fold induction of mRNA levels in human adrenocortical H295R cells and this effect was suppressed by the addition of 25-hydroxycholesterol.	25-hydroxycholesterol	183-204	cytochrome P450 family 51 subfamily A member 1	Homo sapiens	15-20
8619637-9	1996	In human hepatoma HepG2 cells, no effect of cholesterol deprivation was observed; however, the levels of CYP51 mRNA were reduced 4- to 6-fold by the addition of 25-hydroxycholesterol.	25-hydroxycholesterol	161-182	cytochrome P450 family 51 subfamily A member 1	Homo sapiens	105-110
8626488-4	1996	The increase of SE mRNA in HeLa cells grown in LPDS was preventable in a dose-dependent manner by feeding cells with 25-hydroxycholesterol or cholesterol.	25-hydroxycholesterol	117-138	squalene epoxidase	Homo sapiens	16-18
8615754-4	1996	When THP-1 monocytic cells were cultured in the presence of 7beta-hydroxycholesterol (7beta-OH) or 25-hydroxycholesterol (25-OH), prothrombinase, which reflects anionic phospholipid exposure and tissue factor (TF) procoagulant activities, increased in a time- and dose-dependent manner.	25-hydroxycholesterol	99-120	coagulation factor X	Homo sapiens	130-144
8615754-4	1996	When THP-1 monocytic cells were cultured in the presence of 7beta-hydroxycholesterol (7beta-OH) or 25-hydroxycholesterol (25-OH), prothrombinase, which reflects anionic phospholipid exposure and tissue factor (TF) procoagulant activities, increased in a time- and dose-dependent manner.	25-hydroxycholesterol	122-127	coagulation factor X	Homo sapiens	130-144
8615754-4	1996	When THP-1 monocytic cells were cultured in the presence of 7beta-hydroxycholesterol (7beta-OH) or 25-hydroxycholesterol (25-OH), prothrombinase, which reflects anionic phospholipid exposure and tissue factor (TF) procoagulant activities, increased in a time- and dose-dependent manner.	25-hydroxycholesterol	122-127	coagulation factor III, tissue factor	Homo sapiens	195-208
8615754-4	1996	When THP-1 monocytic cells were cultured in the presence of 7beta-hydroxycholesterol (7beta-OH) or 25-hydroxycholesterol (25-OH), prothrombinase, which reflects anionic phospholipid exposure and tissue factor (TF) procoagulant activities, increased in a time- and dose-dependent manner.	25-hydroxycholesterol	122-127	coagulation factor III, tissue factor	Homo sapiens	210-212
8579633-3	1995	We have also assessed 25-hydroxycholesterol for toxicity because it has often been used in studies of 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase inhibition and LDL receptor down-regulation.	25-hydroxycholesterol	22-43	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	102-153
8645354-4	1996	The presence of modified lipoprotein, or of 25-hydroxycholesterol, markedly increased cholesterol esterification in these cells and these effects were potently inhibited by the presence of the ACAT inhibitor, 447C88.	25-hydroxycholesterol	44-65	carboxylesterase 1	Homo sapiens	193-197
8645354-8	1996	Indeed, nCEH activity in these macrophages was much lower than in mouse peritoneal macrophages, and appeared to be down-regulated in the presence of 25-hydroxycholesterol or modified lipoproteins; this loss of nCEH activity was prevented by the ACAT inhibitor 447C88.	25-hydroxycholesterol	149-170	neutral cholesterol ester hydrolase 1	Mus musculus	8-12
8645354-8	1996	Indeed, nCEH activity in these macrophages was much lower than in mouse peritoneal macrophages, and appeared to be down-regulated in the presence of 25-hydroxycholesterol or modified lipoproteins; this loss of nCEH activity was prevented by the ACAT inhibitor 447C88.	25-hydroxycholesterol	149-170	neutral cholesterol ester hydrolase 1	Mus musculus	210-214
8645354-8	1996	Indeed, nCEH activity in these macrophages was much lower than in mouse peritoneal macrophages, and appeared to be down-regulated in the presence of 25-hydroxycholesterol or modified lipoproteins; this loss of nCEH activity was prevented by the ACAT inhibitor 447C88.	25-hydroxycholesterol	149-170	acetyl-Coenzyme A acetyltransferase 1	Mus musculus	245-249
8567968-4	1996	Incubation with 7 beta-hydroxycholesterol and 25-hydroxycholesterol resulted in a 70-75% reduction of LPL mRNA, analyzed by quantitative RT-PCR.	25-hydroxycholesterol	46-67	lipoprotein lipase	Homo sapiens	102-105
8567968-6	1996	LPL activity in the medium was also reduced after exposure of the macrophages to 7 beta-hydroxycholesterol and 25-hydroxycholesterol.	25-hydroxycholesterol	111-132	lipoprotein lipase	Homo sapiens	0-3
8567968-8	1996	There was suppression of LPL mRNA in human monocyte-derived macrophages after incubation with 7 beta-hydroxycholesterol and 25-hydroxycholesterol.	25-hydroxycholesterol	124-145	lipoprotein lipase	Homo sapiens	25-28
8820097-7	1996	The stimulation of ACAT by 25-hydroxycholesterol (20 microgram/ml) caused a 4-fold increase of the cellular cholesteryl ester content and a 2-fold increase of the lipoprotein secretion rate.	25-hydroxycholesterol	27-48	sterol O-acyltransferase 1	Homo sapiens	19-23
8864249-5	1996	The results showed mRNA expressions of LDLR gene were inhibited to 16.1 +/- 4.4%, 33.8 +/- 0.6%, 42.8 +/- 1.8% and 46.9 +/- 3.9% of control by 25-OH, 7-keto, epoxide and triol respectively.	25-hydroxycholesterol	143-148	low-density lipoprotein receptor	Oryctolagus cuniculus	39-43
7989577-8	1994	Incubations with 25-hydroxy cholesterol also caused a dose-dependent stimulation of SCP2 gene expression in macrophages, while incubation with maleylated BSA had no effect.	25-hydroxycholesterol	17-39	sterol carrier protein 2	Rattus norvegicus	84-88
8576635-5	1995	In contrast, incubation of the cells with 25-hydroxycholesterol produced a 30% decrease of LDL receptor expression.	25-hydroxycholesterol	42-63	low density lipoprotein receptor	Homo sapiens	91-103
7642545-2	1995	During studies on the regulation of rat ovarian steroidogenic enzymes by interleukin-1 beta (IL-1 beta), we observed substantial metabolism of 25-hydroxycholesterol to two unusual polar products.	25-hydroxycholesterol	143-164	interleukin 1 beta	Rattus norvegicus	73-91
7642545-2	1995	During studies on the regulation of rat ovarian steroidogenic enzymes by interleukin-1 beta (IL-1 beta), we observed substantial metabolism of 25-hydroxycholesterol to two unusual polar products.	25-hydroxycholesterol	143-164	interleukin 1 beta	Rattus norvegicus	93-102
7642545-7	1995	IL-1 beta-stimulated ovarian 7 alpha-hydroxylase activity (3-10 pmol/min/mg of cellular protein) was nearly 4-fold that of control levels using 25-hydroxycholesterol as substrate.	25-hydroxycholesterol	144-165	interleukin 1 beta	Rattus norvegicus	0-9
7595067-0	1995	Regulation of low density lipoprotein receptor gene expression in HepG2 and Caco2 cells by palmitate, oleate, and 25-hydroxycholesterol.	25-hydroxycholesterol	114-135	low density lipoprotein receptor	Homo sapiens	14-46
7595067-8	1995	The suppressive effect of 25-hydroxycholesterol on LDL-receptor regulation was studied by incubating HepG2 and Caco2 cells grown either in 10% FCS or 10% LPDS for 24 h and then for 0-24 h with various doses of 25-hydroxycholesterol.	25-hydroxycholesterol	26-47	low density lipoprotein receptor	Homo sapiens	51-63
7595067-14	1995	The combination of palmitate (0.8 mM) and 25-hydroxycholesterol (2.5-5 micrograms/ml) suppressed LDL-receptor activity by 65% in HepG2 cells and by 52% in Caco2 cells.	25-hydroxycholesterol	42-63	low density lipoprotein receptor	Homo sapiens	97-109
7713903-2	1995	In Chinese hamster ovary (CHO) cells, brefeldin A (BFA) caused dose-dependent inhibition of 25-hydroxycholesterol-mediated suppression of mRNAs for four sterol-regulated genes: 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase, HMG-CoA synthase, farnesyl-diphosphate synthase, and the low density lipoprotein receptor.	25-hydroxycholesterol	92-113	farnesyl pyrophosphate synthase	Cricetulus griseus	243-272
7713903-2	1995	In Chinese hamster ovary (CHO) cells, brefeldin A (BFA) caused dose-dependent inhibition of 25-hydroxycholesterol-mediated suppression of mRNAs for four sterol-regulated genes: 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase, HMG-CoA synthase, farnesyl-diphosphate synthase, and the low density lipoprotein receptor.	25-hydroxycholesterol	92-113	low-density lipoprotein receptor	Cricetulus griseus	282-314
7713903-4	1995	In the presence of BFA (1 microgram/ml), 25-hydroxycholesterol-mediated suppression of mRNAs for HMG-CoA reductase, the low density lipoprotein receptor, and farnesyl-diphosphate synthase was almost completely blocked.	25-hydroxycholesterol	41-62	3-hydroxy-3-methylglutaryl-coenzyme A reductase	Cricetulus griseus	97-114
7713903-4	1995	In the presence of BFA (1 microgram/ml), 25-hydroxycholesterol-mediated suppression of mRNAs for HMG-CoA reductase, the low density lipoprotein receptor, and farnesyl-diphosphate synthase was almost completely blocked.	25-hydroxycholesterol	41-62	low-density lipoprotein receptor	Cricetulus griseus	120-152
7713903-4	1995	In the presence of BFA (1 microgram/ml), 25-hydroxycholesterol-mediated suppression of mRNAs for HMG-CoA reductase, the low density lipoprotein receptor, and farnesyl-diphosphate synthase was almost completely blocked.	25-hydroxycholesterol	41-62	farnesyl pyrophosphate synthase	Cricetulus griseus	158-187
7713903-8	1995	Monensin was also found to block the effects of 25-hydroxycholesterol on suppression of HMG-CoA reductase.	25-hydroxycholesterol	48-69	3-hydroxy-3-methylglutaryl-coenzyme A reductase	Cricetulus griseus	88-105
7717981-0	1995	3"-untranslated sequences mediate post-transcriptional regulation of 3-hydroxy-3-methylglutaryl-CoA reductase mRNA by 25-hydroxycholesterol.	25-hydroxycholesterol	118-139	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	69-109
7717981-3	1995	296, 859-866], we determined that 25-hydroxycholesterol regulates 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase mRNA through a post-transcriptional mechanism that requires protein synthesis.	25-hydroxycholesterol	34-55	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	66-116
7830271-1	1995	A new series of sterols was synthesized and tested in a CHO cell-based LDL receptor/luciferase (LDLR/Luc) assay to investigate the capability of derepressing the transcription of LDL receptor promoter in the presence of 25-hydroxycholesterol.	25-hydroxycholesterol	220-241	low-density lipoprotein receptor	Cricetulus griseus	179-191
7822296-3	1995	Experiments performed in intact mammalian cells have shown that the rate of cholesteryl ester synthesis in intact cells, as well as the ACAT activity from cell extracts, are greatly activated by the addition of low density lipoprotein (LDL) or oxygenated sterols such as 25-hydroxycholesterol to the growth medium.	25-hydroxycholesterol	271-292	carboxylesterase 1	Homo sapiens	136-140
7897321-11	1994	In addition, rat liver HMG-CoA reductase was inhibited by the administration of a single intragastric dose (1 microgram/kg) of an aqueous solution of 25-hydroxycholesterol.	25-hydroxycholesterol	150-171	3-hydroxy-3-methylglutaryl-CoA reductase	Rattus norvegicus	23-40
8546749-4	1995	Maximum increases in ACAT activity were obtained in macrophages incubated with 25-hydroxycholesterol plus LDL or acetyl-LDL.	25-hydroxycholesterol	79-100	acetyl-CoA acetyltransferase 1	Homo sapiens	21-25
7744865-2	1995	The cholesterol analogue 25-hydroxycholesterol kills animal cells by blocking the proteolytic activation of two sterol-regulated transcription factors designated sterol regulatory element binding protein-1 and -2 (SREBP-1 and SREBP-2).	25-hydroxycholesterol	25-46	sterol regulatory element-binding protein 1	Cricetulus griseus	162-212
7744865-2	1995	The cholesterol analogue 25-hydroxycholesterol kills animal cells by blocking the proteolytic activation of two sterol-regulated transcription factors designated sterol regulatory element binding protein-1 and -2 (SREBP-1 and SREBP-2).	25-hydroxycholesterol	25-46	sterol regulatory element-binding protein 1	Cricetulus griseus	214-221
7744865-2	1995	The cholesterol analogue 25-hydroxycholesterol kills animal cells by blocking the proteolytic activation of two sterol-regulated transcription factors designated sterol regulatory element binding protein-1 and -2 (SREBP-1 and SREBP-2).	25-hydroxycholesterol	25-46	sterol regulatory element-binding protein 2	Cricetulus griseus	226-233
7734449-12	1995	In cells pre-treated with LDL for 24 h, the stimulation was delayed by 4 h. Treatment of cells with 25-hydroxycholesterol completely prevented PG stimulation of HMGR activity.	25-hydroxycholesterol	100-121	3-hydroxy-3-methylglutaryl-CoA reductase	Rattus norvegicus	161-165
7605920-5	1995	Progesterone, a steroid hormone that binds to apoD with high affinity (10(-6) mol l-1) and the oxysterol, 25-hydroxycholesterol which is a potent regulator of cellular cholesterol homeostasis in mammalian cells, differentially stimulated apoD, but not apoE secretion.	25-hydroxycholesterol	106-127	apolipoprotein D	Homo sapiens	238-242
7605920-5	1995	Progesterone, a steroid hormone that binds to apoD with high affinity (10(-6) mol l-1) and the oxysterol, 25-hydroxycholesterol which is a potent regulator of cellular cholesterol homeostasis in mammalian cells, differentially stimulated apoD, but not apoE secretion.	25-hydroxycholesterol	106-127	apolipoprotein E	Homo sapiens	252-256
7775862-4	1995	Pretreatment with 25-OHC (1 microgram/ml) caused a 30% reduction in [3H]cholesterol efflux from L-cells during 8 h of incubation with HDL3.	25-hydroxycholesterol	18-24	high density lipoprotein (HDL) level 3	Mus musculus	134-138