PMID-sentid	Pub_year	Sent_text	compound_name	comp_offset	prot_official_name	organism	prot_offset
32905663-7	2021	Bufalin inhibits the mammalian target of rapamycin (mTOR) activation and subsequently decreases hypoxia-induced factor 1 alpha (HIF-1alpha) level.	bufalin	0-7	mechanistic target of rapamycin kinase	Homo sapiens	21-50
32905663-7	2021	Bufalin inhibits the mammalian target of rapamycin (mTOR) activation and subsequently decreases hypoxia-induced factor 1 alpha (HIF-1alpha) level.	bufalin	0-7	mechanistic target of rapamycin kinase	Homo sapiens	52-56
32905663-7	2021	Bufalin inhibits the mammalian target of rapamycin (mTOR) activation and subsequently decreases hypoxia-induced factor 1 alpha (HIF-1alpha) level.	bufalin	0-7	hypoxia inducible factor 1 subunit alpha	Homo sapiens	128-138
32905663-8	2021	Over-expression of HIF-1alpha could abolish the pro-apoptotic and anti-migration activity of bufalin in cell culture.	bufalin	93-100	hypoxia inducible factor 1 subunit alpha	Homo sapiens	19-29
32905663-10	2021	Bufalin was a potent inhibitor of cell growth and migration in ovarian cancer cells through suppression of mTOR activation and HIF-1alpha induction.	bufalin	0-7	mechanistic target of rapamycin kinase	Homo sapiens	107-111
32905663-10	2021	Bufalin was a potent inhibitor of cell growth and migration in ovarian cancer cells through suppression of mTOR activation and HIF-1alpha induction.	bufalin	0-7	hypoxia inducible factor 1 subunit alpha	Homo sapiens	127-137
33090636-10	2021	Additionally, arenobufagin and bufalin, SRC small molecule inhibitors, can reduce the proliferation and stemness of GBM cells.	bufalin	31-38	SRC proto-oncogene, non-receptor tyrosine kinase	Homo sapiens	40-43
33530915-0	2021	Effect of PI3K/AKT/mTOR Signaling Pathway on Regulating and Controlling the Anti-Invasion and Metastasis of Hepatoma Cells by Bufalin.	bufalin	126-133	AKT serine/threonine kinase 1	Homo sapiens	15-18
33530915-0	2021	Effect of PI3K/AKT/mTOR Signaling Pathway on Regulating and Controlling the Anti-Invasion and Metastasis of Hepatoma Cells by Bufalin.	bufalin	126-133	mechanistic target of rapamycin kinase	Homo sapiens	19-23
33530915-2	2021	OBJECTIVE: In this study, we explored the effect of PI3K/AKT/mTOR autophagy related signaling pathway on regulating and controlling the invasion and metastasis of liver cancer cells by Bufalin.	bufalin	185-192	AKT serine/threonine kinase 1	Homo sapiens	57-60
33530915-2	2021	OBJECTIVE: In this study, we explored the effect of PI3K/AKT/mTOR autophagy related signaling pathway on regulating and controlling the invasion and metastasis of liver cancer cells by Bufalin.	bufalin	185-192	mechanistic target of rapamycin kinase	Homo sapiens	61-65
33530915-7	2021	After inhibiting autophagy, Bufalin significantly inhibited the protein expression of P-AKT, Cyclin D1, MMP-2, MMP-9 and VEGF in HCC-LM3 cells.	bufalin	28-35	cyclin D1	Homo sapiens	93-102
33530915-7	2021	After inhibiting autophagy, Bufalin significantly inhibited the protein expression of P-AKT, Cyclin D1, MMP-2, MMP-9 and VEGF in HCC-LM3 cells.	bufalin	28-35	matrix metallopeptidase 2	Homo sapiens	104-109
33530915-7	2021	After inhibiting autophagy, Bufalin significantly inhibited the protein expression of P-AKT, Cyclin D1, MMP-2, MMP-9 and VEGF in HCC-LM3 cells.	bufalin	28-35	matrix metallopeptidase 9	Homo sapiens	111-116
33530915-7	2021	After inhibiting autophagy, Bufalin significantly inhibited the protein expression of P-AKT, Cyclin D1, MMP-2, MMP-9 and VEGF in HCC-LM3 cells.	bufalin	28-35	vascular endothelial growth factor A	Homo sapiens	121-125
32623699-0	2020	Bufalin inhibits hepatitis B virus-associated hepatocellular carcinoma development through androgen receptor dephosphorylation and cell cycle-related kinase degradation.	bufalin	0-7	androgen receptor	Homo sapiens	91-108
33197852-10	2020	Furthermore, BUF administration to TAA-intoxicated rats modulated the Nrf2 and heme oxygenase 1 (HO-1) genes expression in liver and brain tissues.	bufalin	13-16	NFE2 like bZIP transcription factor 2	Rattus norvegicus	70-74
33197852-10	2020	Furthermore, BUF administration to TAA-intoxicated rats modulated the Nrf2 and heme oxygenase 1 (HO-1) genes expression in liver and brain tissues.	bufalin	13-16	heme oxygenase 1	Rattus norvegicus	79-95
33197852-10	2020	Furthermore, BUF administration to TAA-intoxicated rats modulated the Nrf2 and heme oxygenase 1 (HO-1) genes expression in liver and brain tissues.	bufalin	13-16	heme oxygenase 1	Rattus norvegicus	97-101
33197852-11	2020	The histological evaluation showed that pretreatment of TAA-intoxicated rats with BUF ameliorated the degenerative effects of TAA on liver and brain tissues as well as reduced the activation of cellular apoptotic marker; caspase-3 and glial fibrillary acidic protein (GFAP+) astrocytes.	bufalin	82-85	caspase 3	Rattus norvegicus	221-230
33197852-11	2020	The histological evaluation showed that pretreatment of TAA-intoxicated rats with BUF ameliorated the degenerative effects of TAA on liver and brain tissues as well as reduced the activation of cellular apoptotic marker; caspase-3 and glial fibrillary acidic protein (GFAP+) astrocytes.	bufalin	82-85	glial fibrillary acidic protein	Rattus norvegicus	235-266
33197852-11	2020	The histological evaluation showed that pretreatment of TAA-intoxicated rats with BUF ameliorated the degenerative effects of TAA on liver and brain tissues as well as reduced the activation of cellular apoptotic marker; caspase-3 and glial fibrillary acidic protein (GFAP+) astrocytes.	bufalin	82-85	glial fibrillary acidic protein	Rattus norvegicus	268-272
32623699-12	2020	CONCLUSION: Our results indicate that bufalin acts as a potential anti-HCC therapeutic candidate to block HBx-induced AR/CCRK/beta-catenin signaling by targeting AR and CCRK, which may provide a novel strategy for the treatment of HBV-associated HCC.	bufalin	38-45	cyclin dependent kinase 20	Homo sapiens	118-125
32623699-12	2020	CONCLUSION: Our results indicate that bufalin acts as a potential anti-HCC therapeutic candidate to block HBx-induced AR/CCRK/beta-catenin signaling by targeting AR and CCRK, which may provide a novel strategy for the treatment of HBV-associated HCC.	bufalin	38-45	catenin beta 1	Homo sapiens	126-138
32623699-12	2020	CONCLUSION: Our results indicate that bufalin acts as a potential anti-HCC therapeutic candidate to block HBx-induced AR/CCRK/beta-catenin signaling by targeting AR and CCRK, which may provide a novel strategy for the treatment of HBV-associated HCC.	bufalin	38-45	cyclin dependent kinase 20	Homo sapiens	121-125
32623699-0	2020	Bufalin inhibits hepatitis B virus-associated hepatocellular carcinoma development through androgen receptor dephosphorylation and cell cycle-related kinase degradation.	bufalin	0-7	cyclin dependent kinase 20	Homo sapiens	131-156
32623699-5	2020	HBx-induced oncogenic signaling regulated by bufalin was assessed using PCR array, chromatin immunoprecipitation, site-directed mutagenesis, luciferase reporter, transcription and protein expression assays.	bufalin	45-52	X protein	Hepatitis B virus	0-3
32623699-8	2020	Bufalin significantly inhibited HBx-induced malignant transfromation in vitro and tumorigenicity in vivo.	bufalin	0-7	X protein	Hepatitis B virus	32-35
32623699-9	2020	Androgen receptor (AR) signaling was found to be a target of bufalin resistance to HBV-associated hepatocarcinogenesis.	bufalin	61-68	androgen receptor	Homo sapiens	0-17
32623699-10	2020	We also found that bufalin induced both AR dephosphorylation and cell cycle-related kinase (CCRK) degradation to inhibit beta-catenin/TCF signaling, which subsequently led to cell cycle arrest via cyclin D1 down-regulation and p21 up-regulation, resulting in HCC regression.	bufalin	19-26	cyclin dependent kinase 20	Homo sapiens	65-90
32623699-10	2020	We also found that bufalin induced both AR dephosphorylation and cell cycle-related kinase (CCRK) degradation to inhibit beta-catenin/TCF signaling, which subsequently led to cell cycle arrest via cyclin D1 down-regulation and p21 up-regulation, resulting in HCC regression.	bufalin	19-26	cyclin dependent kinase 20	Homo sapiens	92-96
32623699-10	2020	We also found that bufalin induced both AR dephosphorylation and cell cycle-related kinase (CCRK) degradation to inhibit beta-catenin/TCF signaling, which subsequently led to cell cycle arrest via cyclin D1 down-regulation and p21 up-regulation, resulting in HCC regression.	bufalin	19-26	catenin beta 1	Homo sapiens	121-133
32623699-10	2020	We also found that bufalin induced both AR dephosphorylation and cell cycle-related kinase (CCRK) degradation to inhibit beta-catenin/TCF signaling, which subsequently led to cell cycle arrest via cyclin D1 down-regulation and p21 up-regulation, resulting in HCC regression.	bufalin	19-26	cyclin D1	Homo sapiens	197-206
32623699-10	2020	We also found that bufalin induced both AR dephosphorylation and cell cycle-related kinase (CCRK) degradation to inhibit beta-catenin/TCF signaling, which subsequently led to cell cycle arrest via cyclin D1 down-regulation and p21 up-regulation, resulting in HCC regression.	bufalin	19-26	cyclin dependent kinase inhibitor 1A	Homo sapiens	227-230
32623699-11	2020	Furthermore, we found that bufalin reduced > 60% diethylnitrosamine-induced hepatocarcinogenesis in HBx transgenic mice, and improved the sensitivity of refractory HBV-associated HCC cells to sorafenib treatment.	bufalin	27-34	X protein	Hepatitis B virus	100-103
32623699-12	2020	CONCLUSION: Our results indicate that bufalin acts as a potential anti-HCC therapeutic candidate to block HBx-induced AR/CCRK/beta-catenin signaling by targeting AR and CCRK, which may provide a novel strategy for the treatment of HBV-associated HCC.	bufalin	38-45	X protein	Hepatitis B virus	106-109
32939944-3	2020	Then the glycosylation of the bufalin was achieved with the synthesized sugar donor under the catalysis of two glycosyltransferase s (Loki and ASP).	bufalin	30-37	assembly factor for spindle microtubules	Homo sapiens	143-146
32934738-0	2020	Bufalin attenuates triple-negative breast cancer cell stemness by inhibiting the expression of SOX2/OCT4.	bufalin	0-7	SRY-box transcription factor 2	Homo sapiens	95-99
32934738-0	2020	Bufalin attenuates triple-negative breast cancer cell stemness by inhibiting the expression of SOX2/OCT4.	bufalin	0-7	POU class 5 homeobox 1	Homo sapiens	100-104
32934738-6	2020	A mechanistic study suggested that bufalin may suppress the proliferation of TNBC stem cells by inhibiting the expression of octamer-binding transcription factor 4 (OCT4) and sex determining region Y-box 2 (SOX2) in MDA-MB-231 and HCC-1937 cells.	bufalin	35-42	POU class 5 homeobox 1	Homo sapiens	125-163
32934738-6	2020	A mechanistic study suggested that bufalin may suppress the proliferation of TNBC stem cells by inhibiting the expression of octamer-binding transcription factor 4 (OCT4) and sex determining region Y-box 2 (SOX2) in MDA-MB-231 and HCC-1937 cells.	bufalin	35-42	POU class 5 homeobox 1	Homo sapiens	165-169
32934738-6	2020	A mechanistic study suggested that bufalin may suppress the proliferation of TNBC stem cells by inhibiting the expression of octamer-binding transcription factor 4 (OCT4) and sex determining region Y-box 2 (SOX2) in MDA-MB-231 and HCC-1937 cells.	bufalin	35-42	SRY-box transcription factor 2	Homo sapiens	175-205
32934738-6	2020	A mechanistic study suggested that bufalin may suppress the proliferation of TNBC stem cells by inhibiting the expression of octamer-binding transcription factor 4 (OCT4) and sex determining region Y-box 2 (SOX2) in MDA-MB-231 and HCC-1937 cells.	bufalin	35-42	SRY-box transcription factor 2	Homo sapiens	207-211
32934738-7	2020	These results indicated that bufalin may hold promise as a therapeutic agent in TNBC, and its effects may be mediated through the SOX2/OCT4 axis.	bufalin	29-36	SRY-box transcription factor 2	Homo sapiens	130-134
32934738-7	2020	These results indicated that bufalin may hold promise as a therapeutic agent in TNBC, and its effects may be mediated through the SOX2/OCT4 axis.	bufalin	29-36	POU class 5 homeobox 1	Homo sapiens	135-139
32823854-3	2020	In vivo (rodent) experiments proved cardiac anti-fibrotic effects of the natural compounds bufalin and lycorine by downregulating miRNA-671-5p, associated with a theoretic increase in the tissue level of circRNA CDR1as.	bufalin	91-98	CDR1 antisense RNA	Homo sapiens	212-218
33155194-4	2020	Then, the anti-tumor effect of miR-127-3p on EOC cells was explored through the in vitro cell proliferation assay, bufalin sensitivity assay, wound healing assay, and invasion assay.	bufalin	115-122	microRNA 1273a	Homo sapiens	31-41
33155194-8	2020	After lentivirus-mediated overexpression of miR-127-3p, in vitro proliferation and invasion of EOC cells were inhibited, and the sensitivity to bufalin was enhanced (p<0.05).	bufalin	144-151	microRNA 1273a	Homo sapiens	44-54
33155194-10	2020	Moreover, the upregulation of MAPK4 inhibited the anti-tumor effect of miR-127-3p on EOC, manifested as the remarkably enhanced cell proliferation and migration (p<0.05), and the weakened sensitivity to bufalin (p<0.01).	bufalin	203-210	mitogen-activated protein kinase 4	Homo sapiens	30-35
33155194-10	2020	Moreover, the upregulation of MAPK4 inhibited the anti-tumor effect of miR-127-3p on EOC, manifested as the remarkably enhanced cell proliferation and migration (p<0.05), and the weakened sensitivity to bufalin (p<0.01).	bufalin	203-210	microRNA 1273a	Homo sapiens	71-81
32823854-7	2020	Bufalin treatment additionally led to smaller end-diastolic volumes, higher LV ejection fraction (EF), and increased expression of CDR1as of the AMI region.	bufalin	0-7	CDR1 antisense RNA	Homo sapiens	131-137
31669257-2	2020	Recent data indicate that bufalin binds to the pig kidney NKA in a way different from ouabain and digoxin, raising the question of a putative class difference between bufadienolides and cardenolides.	bufalin	26-33	tachykinin precursor 1	Homo sapiens	58-61
32581545-8	2020	Bufalin triggered cell death by upregulating tumor necrosis factor (TNF) -alpha, TNF receptor 1 (TNFR1) and receptor-interacting protein 1 (RIPK1).	bufalin	0-7	tumor necrosis factor	Homo sapiens	45-79
32581545-8	2020	Bufalin triggered cell death by upregulating tumor necrosis factor (TNF) -alpha, TNF receptor 1 (TNFR1) and receptor-interacting protein 1 (RIPK1).	bufalin	0-7	TNF receptor superfamily member 1A	Homo sapiens	81-95
32581545-8	2020	Bufalin triggered cell death by upregulating tumor necrosis factor (TNF) -alpha, TNF receptor 1 (TNFR1) and receptor-interacting protein 1 (RIPK1).	bufalin	0-7	TNF receptor superfamily member 1A	Homo sapiens	97-102
32581545-8	2020	Bufalin triggered cell death by upregulating tumor necrosis factor (TNF) -alpha, TNF receptor 1 (TNFR1) and receptor-interacting protein 1 (RIPK1).	bufalin	0-7	receptor interacting serine/threonine kinase 1	Homo sapiens	108-138
32581545-8	2020	Bufalin triggered cell death by upregulating tumor necrosis factor (TNF) -alpha, TNF receptor 1 (TNFR1) and receptor-interacting protein 1 (RIPK1).	bufalin	0-7	receptor interacting serine/threonine kinase 1	Homo sapiens	140-145
32581545-13	2020	Cytotoxicity of bufalin to caspase-8 knockdown cell lines made control cell lines more sensitive to bufalin in their mixture.	bufalin	16-23	caspase 8	Homo sapiens	27-36
32581545-13	2020	Cytotoxicity of bufalin to caspase-8 knockdown cell lines made control cell lines more sensitive to bufalin in their mixture.	bufalin	100-107	caspase 8	Homo sapiens	27-36
32219334-0	2020	Bufalin downregulates Axl expression to inhibit cell proliferation and induce apoptosis in non-small cell lung cancer cells.	bufalin	0-7	AXL receptor tyrosine kinase	Homo sapiens	22-25
32219334-2	2020	In this study, we demonstrated that the anti-cancer activity of bufalin, a major bioactive component of the Chinese traditional medicine Chan Su, is mediated by the downregulation of Axl in non-small-cell lung cancer (NSCLC) cells.	bufalin	64-71	AXL receptor tyrosine kinase	Homo sapiens	183-186
32219334-4	2020	Next, we found that the protein level of Axl was decreased in proportion to the concentration of bufalin in both A549 and H460 cells.	bufalin	97-104	AXL receptor tyrosine kinase	Homo sapiens	41-44
32219334-7	2020	Axl overexpression attenuated the effect of bufalin in inhibiting cell proliferation and colony formation and inducing apoptosis in H460 cells, while knockdown of Axl gene expression induced the opposite effect.	bufalin	44-51	AXL receptor tyrosine kinase	Homo sapiens	0-3
32362830-0	2020	Silencing c-Myc Enhances the Antitumor Activity of Bufalin by Suppressing the HIF-1alpha/SDF-1/CXCR4 Pathway in Pancreatic Cancer Cells.	bufalin	51-58	MYC proto-oncogene, bHLH transcription factor	Homo sapiens	10-15
32362830-0	2020	Silencing c-Myc Enhances the Antitumor Activity of Bufalin by Suppressing the HIF-1alpha/SDF-1/CXCR4 Pathway in Pancreatic Cancer Cells.	bufalin	51-58	hypoxia inducible factor 1 subunit alpha	Homo sapiens	78-88
32362830-0	2020	Silencing c-Myc Enhances the Antitumor Activity of Bufalin by Suppressing the HIF-1alpha/SDF-1/CXCR4 Pathway in Pancreatic Cancer Cells.	bufalin	51-58	C-X-C motif chemokine ligand 12	Homo sapiens	89-94
32362830-0	2020	Silencing c-Myc Enhances the Antitumor Activity of Bufalin by Suppressing the HIF-1alpha/SDF-1/CXCR4 Pathway in Pancreatic Cancer Cells.	bufalin	51-58	C-X-C motif chemokine receptor 4	Homo sapiens	95-100
32362830-5	2020	The present study aimed to investigate the role of c-Myc in the antitumor activity of bufalin in pancreatic cancer.	bufalin	86-93	MYC proto-oncogene, bHLH transcription factor	Homo sapiens	51-56
32362830-8	2020	The effect of c-Myc on bufalin-induced inhibition of cell proliferation was detected via CCK-8 assay.	bufalin	23-30	MYC proto-oncogene, bHLH transcription factor	Homo sapiens	14-19
32362830-15	2020	Similarly, c-Myc downregulation enhanced the effect of bufalin on cell cycle arrest, apoptosis, and the invasion and migration of pancreatic cancer cell in vitro.	bufalin	55-62	MYC proto-oncogene, bHLH transcription factor	Homo sapiens	11-16
32362830-17	2020	The in vivo studies verified the results that c-Myc enhances the effect of bufalin through regulation of the HIF-1alpha/SDF-1/CXCR4 pathway.	bufalin	75-82	MYC proto-oncogene, bHLH transcription factor	Homo sapiens	46-51
32362830-17	2020	The in vivo studies verified the results that c-Myc enhances the effect of bufalin through regulation of the HIF-1alpha/SDF-1/CXCR4 pathway.	bufalin	75-82	hypoxia inducible factor 1 subunit alpha	Homo sapiens	109-119
32362830-17	2020	The in vivo studies verified the results that c-Myc enhances the effect of bufalin through regulation of the HIF-1alpha/SDF-1/CXCR4 pathway.	bufalin	75-82	C-X-C motif chemokine ligand 12	Homo sapiens	120-125
32362830-17	2020	The in vivo studies verified the results that c-Myc enhances the effect of bufalin through regulation of the HIF-1alpha/SDF-1/CXCR4 pathway.	bufalin	75-82	C-X-C motif chemokine receptor 4	Homo sapiens	126-131
32362830-18	2020	Conclusions: Downregulation of c-Myc enhanced the antitumor activity of bufalin in pancreatic cancer cells by suppressing the HIF-1alpha/SDF-1/CXCR4 pathway.	bufalin	72-79	MYC proto-oncogene, bHLH transcription factor	Homo sapiens	31-36
32362830-18	2020	Conclusions: Downregulation of c-Myc enhanced the antitumor activity of bufalin in pancreatic cancer cells by suppressing the HIF-1alpha/SDF-1/CXCR4 pathway.	bufalin	72-79	hypoxia inducible factor 1 subunit alpha	Homo sapiens	126-136
32362830-18	2020	Conclusions: Downregulation of c-Myc enhanced the antitumor activity of bufalin in pancreatic cancer cells by suppressing the HIF-1alpha/SDF-1/CXCR4 pathway.	bufalin	72-79	C-X-C motif chemokine ligand 12	Homo sapiens	137-142
32362830-18	2020	Conclusions: Downregulation of c-Myc enhanced the antitumor activity of bufalin in pancreatic cancer cells by suppressing the HIF-1alpha/SDF-1/CXCR4 pathway.	bufalin	72-79	C-X-C motif chemokine receptor 4	Homo sapiens	143-148
32362830-19	2020	These findings indicate that c-Myc inhibitors could enhance the clinical therapeutic effect of bufalin and may expand the clinical application of bufalin accordingly.	bufalin	95-102	MYC proto-oncogene, bHLH transcription factor	Homo sapiens	29-34
32362830-19	2020	These findings indicate that c-Myc inhibitors could enhance the clinical therapeutic effect of bufalin and may expand the clinical application of bufalin accordingly.	bufalin	146-153	MYC proto-oncogene, bHLH transcription factor	Homo sapiens	29-34
33148004-14	2020	Bufalin could inhibit the proliferation, invasion, and metastasis of cancer cells through multiple signaling pathways, such as PI3K/AKT, Hedgehog, MAPK/JNK, Wnt/[Formula: see text]-catenin, TGF-[Formula: see text]/Smad, Integrin signaling pathway, and NF-KB signaling pathway via KEGG analysis.	bufalin	0-7	AKT serine/threonine kinase 1	Homo sapiens	132-135
33148004-14	2020	Bufalin could inhibit the proliferation, invasion, and metastasis of cancer cells through multiple signaling pathways, such as PI3K/AKT, Hedgehog, MAPK/JNK, Wnt/[Formula: see text]-catenin, TGF-[Formula: see text]/Smad, Integrin signaling pathway, and NF-KB signaling pathway via KEGG analysis.	bufalin	0-7	mitogen-activated protein kinase 8	Homo sapiens	152-155
32231716-7	2020	Western blot assay showed that Bufalin inhibited MEK/ERK and PI3-K/AKT signaling pathways by inhibiting the expression of p-c-Met, which in turn affected the expression of apoptosis-related protein Mcl-1, and the invasion-associated proteins E-cadherin, MMP9 and Snail.	bufalin	31-38	mitogen-activated protein kinase kinase 7	Homo sapiens	49-52
32231716-7	2020	Western blot assay showed that Bufalin inhibited MEK/ERK and PI3-K/AKT signaling pathways by inhibiting the expression of p-c-Met, which in turn affected the expression of apoptosis-related protein Mcl-1, and the invasion-associated proteins E-cadherin, MMP9 and Snail.	bufalin	31-38	mitogen-activated protein kinase 1	Homo sapiens	53-56
32231716-7	2020	Western blot assay showed that Bufalin inhibited MEK/ERK and PI3-K/AKT signaling pathways by inhibiting the expression of p-c-Met, which in turn affected the expression of apoptosis-related protein Mcl-1, and the invasion-associated proteins E-cadherin, MMP9 and Snail.	bufalin	31-38	AKT serine/threonine kinase 1	Homo sapiens	67-70
32231716-7	2020	Western blot assay showed that Bufalin inhibited MEK/ERK and PI3-K/AKT signaling pathways by inhibiting the expression of p-c-Met, which in turn affected the expression of apoptosis-related protein Mcl-1, and the invasion-associated proteins E-cadherin, MMP9 and Snail.	bufalin	31-38	MET proto-oncogene, receptor tyrosine kinase	Homo sapiens	124-129
32231716-7	2020	Western blot assay showed that Bufalin inhibited MEK/ERK and PI3-K/AKT signaling pathways by inhibiting the expression of p-c-Met, which in turn affected the expression of apoptosis-related protein Mcl-1, and the invasion-associated proteins E-cadherin, MMP9 and Snail.	bufalin	31-38	MCL1 apoptosis regulator, BCL2 family member	Homo sapiens	198-203
32231716-7	2020	Western blot assay showed that Bufalin inhibited MEK/ERK and PI3-K/AKT signaling pathways by inhibiting the expression of p-c-Met, which in turn affected the expression of apoptosis-related protein Mcl-1, and the invasion-associated proteins E-cadherin, MMP9 and Snail.	bufalin	31-38	cadherin 1	Homo sapiens	242-252
32231716-7	2020	Western blot assay showed that Bufalin inhibited MEK/ERK and PI3-K/AKT signaling pathways by inhibiting the expression of p-c-Met, which in turn affected the expression of apoptosis-related protein Mcl-1, and the invasion-associated proteins E-cadherin, MMP9 and Snail.	bufalin	31-38	matrix metallopeptidase 9	Homo sapiens	254-258
32231716-7	2020	Western blot assay showed that Bufalin inhibited MEK/ERK and PI3-K/AKT signaling pathways by inhibiting the expression of p-c-Met, which in turn affected the expression of apoptosis-related protein Mcl-1, and the invasion-associated proteins E-cadherin, MMP9 and Snail.	bufalin	31-38	snail family transcriptional repressor 1	Homo sapiens	263-268
30739221-0	2019	The sodium pump alpha1 subunit regulates bufalin sensitivity of human glioblastoma cells through the p53 signaling pathway.	bufalin	41-48	tumor protein p53	Homo sapiens	101-104
30739221-8	2019	The critical roles of the sodium pump alpha1 subunit (ATP1A1) in mediating the XPO1-targeted anticancer effect of bufalin in human glioma were further confirmed.	bufalin	114-121	ATPase Na+/K+ transporting subunit alpha 1	Homo sapiens	54-60
30739221-8	2019	The critical roles of the sodium pump alpha1 subunit (ATP1A1) in mediating the XPO1-targeted anticancer effect of bufalin in human glioma were further confirmed.	bufalin	114-121	exportin 1	Homo sapiens	79-83
30739221-9	2019	Mechanistic studies confirmed the important roles of Src and p53 signaling in mediating bufalin-induced apoptosis.	bufalin	88-95	SRC proto-oncogene, non-receptor tyrosine kinase	Homo sapiens	53-56
30739221-9	2019	Mechanistic studies confirmed the important roles of Src and p53 signaling in mediating bufalin-induced apoptosis.	bufalin	88-95	tumor protein p53	Homo sapiens	61-64
30739221-11	2019	In conclusion, our study indicated that therapies targeting the ATP1A1 and p53 signaling-mediated mitochondrial apoptotic pathways regulated by bufalin might be potential treatments for human glioma, and these findings will provide molecular bases for developing bufalin into a drug candidate for the treatment of malignant glioma.	bufalin	144-151	ATPase Na+/K+ transporting subunit alpha 1	Homo sapiens	64-70
30739221-11	2019	In conclusion, our study indicated that therapies targeting the ATP1A1 and p53 signaling-mediated mitochondrial apoptotic pathways regulated by bufalin might be potential treatments for human glioma, and these findings will provide molecular bases for developing bufalin into a drug candidate for the treatment of malignant glioma.	bufalin	144-151	tumor protein p53	Homo sapiens	75-78
30739221-11	2019	In conclusion, our study indicated that therapies targeting the ATP1A1 and p53 signaling-mediated mitochondrial apoptotic pathways regulated by bufalin might be potential treatments for human glioma, and these findings will provide molecular bases for developing bufalin into a drug candidate for the treatment of malignant glioma.	bufalin	263-270	ATPase Na+/K+ transporting subunit alpha 1	Homo sapiens	64-70
30739221-11	2019	In conclusion, our study indicated that therapies targeting the ATP1A1 and p53 signaling-mediated mitochondrial apoptotic pathways regulated by bufalin might be potential treatments for human glioma, and these findings will provide molecular bases for developing bufalin into a drug candidate for the treatment of malignant glioma.	bufalin	263-270	tumor protein p53	Homo sapiens	75-78
31028291-6	2019	To explore the role of lncRNAs in the antimetastatic activity of bufalin, we used an lncRNA microarray and found that HOX transcript antisense RNA (HOTAIR) was the most markedly downregulated lncRNA in bufalin-treated PCa cells.	bufalin	65-72	HOX transcript antisense RNA	Homo sapiens	148-154
31431500-5	2019	Bufalin also suppressed the expression of proinflammatory mediators [reduced levels of cyclooxygenase-2, tumor TNFalpha, IL1beta, IL6, C-X-C motif chemokine ligand (CXCL)-1, CXCL-2, and CXCL-5] in the colitis-associated colorectal cancer model.	bufalin	0-7	prostaglandin-endoperoxide synthase 2	Mus musculus	87-103
31431500-5	2019	Bufalin also suppressed the expression of proinflammatory mediators [reduced levels of cyclooxygenase-2, tumor TNFalpha, IL1beta, IL6, C-X-C motif chemokine ligand (CXCL)-1, CXCL-2, and CXCL-5] in the colitis-associated colorectal cancer model.	bufalin	0-7	interleukin 1 alpha	Mus musculus	121-128
31431500-5	2019	Bufalin also suppressed the expression of proinflammatory mediators [reduced levels of cyclooxygenase-2, tumor TNFalpha, IL1beta, IL6, C-X-C motif chemokine ligand (CXCL)-1, CXCL-2, and CXCL-5] in the colitis-associated colorectal cancer model.	bufalin	0-7	interleukin 6	Mus musculus	130-133
31431500-5	2019	Bufalin also suppressed the expression of proinflammatory mediators [reduced levels of cyclooxygenase-2, tumor TNFalpha, IL1beta, IL6, C-X-C motif chemokine ligand (CXCL)-1, CXCL-2, and CXCL-5] in the colitis-associated colorectal cancer model.	bufalin	0-7	chemokine (C-X-C motif) ligand 1	Mus musculus	135-172
31431500-5	2019	Bufalin also suppressed the expression of proinflammatory mediators [reduced levels of cyclooxygenase-2, tumor TNFalpha, IL1beta, IL6, C-X-C motif chemokine ligand (CXCL)-1, CXCL-2, and CXCL-5] in the colitis-associated colorectal cancer model.	bufalin	0-7	chemokine (C-X-C motif) ligand 2	Mus musculus	174-180
31431500-5	2019	Bufalin also suppressed the expression of proinflammatory mediators [reduced levels of cyclooxygenase-2, tumor TNFalpha, IL1beta, IL6, C-X-C motif chemokine ligand (CXCL)-1, CXCL-2, and CXCL-5] in the colitis-associated colorectal cancer model.	bufalin	0-7	chemokine (C-X-C motif) ligand 5	Mus musculus	186-192
31301315-2	2019	Bufalin, a natural digoxin-like ingredient isolated from a traditional Chinese medicine Chan Su, has been shown to downregulate MCL-1 in NSCLC cells.	bufalin	0-7	MCL1 apoptosis regulator, BCL2 family member	Homo sapiens	128-133
31301315-7	2019	In osimertinib-resistant cells, bufalin modulates Ku70-mediated MCL-1 degradation, but not MEK/ERK/MCL-1 signaling.	bufalin	32-39	MCL1 apoptosis regulator, BCL2 family member	Homo sapiens	64-69
31301315-8	2019	In conclusion, our study suggests that bufalin eliminates resistance to osimertinib by inhibiting Ku70-mediated MCL-1 overexpression, indicating that a combination of osimertinib and bufalin could be an effective additional treatment to overcome acquired resistance to osimertinib in NSCLC cells.	bufalin	39-46	MCL1 apoptosis regulator, BCL2 family member	Homo sapiens	112-117
31028291-6	2019	To explore the role of lncRNAs in the antimetastatic activity of bufalin, we used an lncRNA microarray and found that HOX transcript antisense RNA (HOTAIR) was the most markedly downregulated lncRNA in bufalin-treated PCa cells.	bufalin	202-209	HOX transcript antisense RNA	Homo sapiens	148-154
30829654-7	2019	Our results showed that bufalin reduced cellular viability and promoted reactive oxygen species production, which could be inhibited by Nec-1 and N-acetylcysteine.	bufalin	24-31	proprotein convertase subtilisin/kexin type 1	Homo sapiens	136-141
30829654-9	2019	Western blot analysis showed that bufalin induced necroptosis by upregulating the necroptosis mediator RIP1 and the RIP1/RIP3/PGAM5 pathway.	bufalin	34-41	receptor interacting serine/threonine kinase 1	Homo sapiens	103-107
30829654-9	2019	Western blot analysis showed that bufalin induced necroptosis by upregulating the necroptosis mediator RIP1 and the RIP1/RIP3/PGAM5 pathway.	bufalin	34-41	receptor interacting serine/threonine kinase 1	Homo sapiens	116-120
30829654-9	2019	Western blot analysis showed that bufalin induced necroptosis by upregulating the necroptosis mediator RIP1 and the RIP1/RIP3/PGAM5 pathway.	bufalin	34-41	myosin phosphatase Rho interacting protein	Homo sapiens	121-125
30829654-9	2019	Western blot analysis showed that bufalin induced necroptosis by upregulating the necroptosis mediator RIP1 and the RIP1/RIP3/PGAM5 pathway.	bufalin	34-41	PGAM family member 5, mitochondrial serine/threonine protein phosphatase	Homo sapiens	126-131
30829654-10	2019	Taken together, these findings indicated that bufalin induces breast cancer cell necroptosis by targeting the RIP1/RIP3/PGAM5 pathway.	bufalin	46-53	receptor interacting serine/threonine kinase 1	Homo sapiens	110-114
30955024-4	2019	MATERIAL AND METHODS We used flow cytometry to evaluate intracellular ADM concentration, and RT-PCR and Western blot analysis were used to assess the effect of nuclear factor erythroid-2-related factor 2 (Nrf2) bufalin-related resistance gene expression.	bufalin	211-218	NFE2 like bZIP transcription factor 2	Homo sapiens	160-203
30829654-10	2019	Taken together, these findings indicated that bufalin induces breast cancer cell necroptosis by targeting the RIP1/RIP3/PGAM5 pathway.	bufalin	46-53	myosin phosphatase Rho interacting protein	Homo sapiens	115-119
30829654-10	2019	Taken together, these findings indicated that bufalin induces breast cancer cell necroptosis by targeting the RIP1/RIP3/PGAM5 pathway.	bufalin	46-53	PGAM family member 5, mitochondrial serine/threonine protein phosphatase	Homo sapiens	120-125
30777466-0	2019	Bufalin induces apoptosis via mitochondrial ROS-mediated caspase-3 activation in HCT-116 and SW620 human colon cancer cells.	bufalin	0-7	caspase 3	Homo sapiens	57-66
30777466-9	2019	Bufalin decreased cell viability, increased cell death as well as caspase-3 downstream target (cleaved PARP) accumulation, and these actions were significantly blocked by pan-caspase inhibitor zVAD-FMK.	bufalin	0-7	caspase 3	Homo sapiens	66-75
30777466-9	2019	Bufalin decreased cell viability, increased cell death as well as caspase-3 downstream target (cleaved PARP) accumulation, and these actions were significantly blocked by pan-caspase inhibitor zVAD-FMK.	bufalin	0-7	poly(ADP-ribose) polymerase 1	Homo sapiens	103-107
30777466-12	2019	CONCLUSION: Bufalin significantly induces apoptosis in HCT-116 and SW620 colon cancer cells via mitochondrial ROS-mediated caspase-3 activation.	bufalin	12-19	caspase 3	Homo sapiens	123-132
30955024-6	2019	RESULTS We found that bufalin can increase the concentration of Adriamycin (ADM) in K562/A02 cells by inhibiting the expression of Nrf2 and related drug resistance factors.	bufalin	22-29	NFE2 like bZIP transcription factor 2	Homo sapiens	131-135
30955024-7	2019	The results showed that bufalin induced apoptosis of K562/A02 cells by the IRE1alpha/TRAF2/JNK/caspase-12 pathway.	bufalin	24-31	endoplasmic reticulum to nucleus signaling 1	Homo sapiens	75-84
30955024-7	2019	The results showed that bufalin induced apoptosis of K562/A02 cells by the IRE1alpha/TRAF2/JNK/caspase-12 pathway.	bufalin	24-31	TNF receptor associated factor 2	Homo sapiens	85-90
30955024-7	2019	The results showed that bufalin induced apoptosis of K562/A02 cells by the IRE1alpha/TRAF2/JNK/caspase-12 pathway.	bufalin	24-31	mitogen-activated protein kinase 8	Homo sapiens	91-94
30955024-8	2019	CONCLUSIONS These results suggest bufalin can reverse drug resistance in K562/A02 cells and that it induces apoptosis of K562/A02 cells by the IRE1alpha/TRAF2/JNK/caspase-12 pathway.	bufalin	34-41	endoplasmic reticulum to nucleus signaling 1	Homo sapiens	143-152
30955024-8	2019	CONCLUSIONS These results suggest bufalin can reverse drug resistance in K562/A02 cells and that it induces apoptosis of K562/A02 cells by the IRE1alpha/TRAF2/JNK/caspase-12 pathway.	bufalin	34-41	TNF receptor associated factor 2	Homo sapiens	153-158
30955024-8	2019	CONCLUSIONS These results suggest bufalin can reverse drug resistance in K562/A02 cells and that it induces apoptosis of K562/A02 cells by the IRE1alpha/TRAF2/JNK/caspase-12 pathway.	bufalin	34-41	mitogen-activated protein kinase 8	Homo sapiens	159-162
29793559-8	2019	Bufalin was found to induce the apoptosis of GSCs by upregulating the expression of the apoptotic proteins cleaved caspase 3 and poly(ADP-ribose) polymerase (PARP) and by downregulating the expression of human telomerase reverse transcriptase, which is a marker of telomerase activity.	bufalin	0-7	poly(ADP-ribose) polymerase 1	Homo sapiens	129-156
29793559-8	2019	Bufalin was found to induce the apoptosis of GSCs by upregulating the expression of the apoptotic proteins cleaved caspase 3 and poly(ADP-ribose) polymerase (PARP) and by downregulating the expression of human telomerase reverse transcriptase, which is a marker of telomerase activity.	bufalin	0-7	poly(ADP-ribose) polymerase 1	Homo sapiens	158-162
30863083-12	2019	Biochemical analysis revealed that HIFU+bufalin treatment elevated PARP expression and increased caspase-8 activation in MiaPaCa2 and Panc-1 cells.	bufalin	40-47	collagen type XI alpha 2 chain	Homo sapiens	67-71
30863083-12	2019	Biochemical analysis revealed that HIFU+bufalin treatment elevated PARP expression and increased caspase-8 activation in MiaPaCa2 and Panc-1 cells.	bufalin	40-47	caspase 8	Homo sapiens	97-106
30863083-14	2019	HIFU+bufalin treatment decreased Ki67 staining and increased activated caspase-3 and caspase 8 staining, when compared with HIFU or bufalin treatment alone in mouse tumors.	bufalin	5-12	caspase 3	Mus musculus	71-80
30863083-14	2019	HIFU+bufalin treatment decreased Ki67 staining and increased activated caspase-3 and caspase 8 staining, when compared with HIFU or bufalin treatment alone in mouse tumors.	bufalin	5-12	caspase 8	Mus musculus	85-94
29915283-10	2018	Hsa-miR-3129 upregulation had significant anti-cancer effects by inhibiting EOC proliferation, increasing bufalin chemoresistance, and suppressing xenotransplantation.	bufalin	106-113	microRNA 3129	Homo sapiens	4-12
30468278-0	2019	Bufalin induces protective autophagy by Cbl-b regulating mTOR and ERK signaling pathways in gastric cancer cells.	bufalin	0-7	Cbl proto-oncogene B	Homo sapiens	40-45
30468278-0	2019	Bufalin induces protective autophagy by Cbl-b regulating mTOR and ERK signaling pathways in gastric cancer cells.	bufalin	0-7	mechanistic target of rapamycin kinase	Homo sapiens	57-61
30468278-0	2019	Bufalin induces protective autophagy by Cbl-b regulating mTOR and ERK signaling pathways in gastric cancer cells.	bufalin	0-7	mitogen-activated protein kinase 3	Homo sapiens	66-69
30468278-7	2019	Moreover, phosphorylated Akt, mTOR, and p70S6K were all significantly decreased, while phosphorylated ERK1/2 was increased by bufalin.	bufalin	126-133	mitogen-activated protein kinase 3	Homo sapiens	102-108
30468278-10	2019	Therefore, our data provide evidence that bufalin induces autophagy in MGC803 cells via both Akt/mTOR/p70S6K and ERK signaling pathways, and Cbl-b-mediated suppression of mTOR and activation of ERK1/2 might play an important role.	bufalin	42-49	AKT serine/threonine kinase 1	Homo sapiens	93-96
30468278-10	2019	Therefore, our data provide evidence that bufalin induces autophagy in MGC803 cells via both Akt/mTOR/p70S6K and ERK signaling pathways, and Cbl-b-mediated suppression of mTOR and activation of ERK1/2 might play an important role.	bufalin	42-49	mechanistic target of rapamycin kinase	Homo sapiens	97-101
30468278-10	2019	Therefore, our data provide evidence that bufalin induces autophagy in MGC803 cells via both Akt/mTOR/p70S6K and ERK signaling pathways, and Cbl-b-mediated suppression of mTOR and activation of ERK1/2 might play an important role.	bufalin	42-49	ribosomal protein S6 kinase B1	Homo sapiens	102-108
30468278-10	2019	Therefore, our data provide evidence that bufalin induces autophagy in MGC803 cells via both Akt/mTOR/p70S6K and ERK signaling pathways, and Cbl-b-mediated suppression of mTOR and activation of ERK1/2 might play an important role.	bufalin	42-49	mitogen-activated protein kinase 3	Homo sapiens	113-116
30468278-10	2019	Therefore, our data provide evidence that bufalin induces autophagy in MGC803 cells via both Akt/mTOR/p70S6K and ERK signaling pathways, and Cbl-b-mediated suppression of mTOR and activation of ERK1/2 might play an important role.	bufalin	42-49	mechanistic target of rapamycin kinase	Homo sapiens	171-175
30468278-10	2019	Therefore, our data provide evidence that bufalin induces autophagy in MGC803 cells via both Akt/mTOR/p70S6K and ERK signaling pathways, and Cbl-b-mediated suppression of mTOR and activation of ERK1/2 might play an important role.	bufalin	42-49	mitogen-activated protein kinase 3	Homo sapiens	194-200
30596555-0	2019	Bufalin-Loaded CaP/DPPE-PEG-EGF Nanospheres: Preparation, Cellular Uptake, Distribution, and Anti-Tumor Effects.	bufalin	0-7	epidermal growth factor	Mus musculus	28-31
30596555-4	2019	Epidermal growth factor (EGF)-modified nanospheres with encapsulated bufalin showed increased toxicity in colorectal cancer cells, inhibition of cell proliferation, and induction of apoptosis relative to the non-EGF-modified nanospheres.	bufalin	69-76	epidermal growth factor	Mus musculus	0-23
30596555-4	2019	Epidermal growth factor (EGF)-modified nanospheres with encapsulated bufalin showed increased toxicity in colorectal cancer cells, inhibition of cell proliferation, and induction of apoptosis relative to the non-EGF-modified nanospheres.	bufalin	69-76	epidermal growth factor	Mus musculus	25-28
30596555-6	2019	In vitro release data showed that bufalin loaded in calcium phosphate/DPPE-PEG-EGF hybrid porous nanospheres was released more slowly from dialysis membranes than free bufalin.	bufalin	34-41	epidermal growth factor	Mus musculus	79-82
30596555-7	2019	According to our results, EGF-modified nanospheres containing bufalin show improved anti-tumor effects on colon cancer in nude mice, but without severe side effects.	bufalin	62-69	epidermal growth factor	Mus musculus	26-29
30612454-0	2019	Bufalin Induces Apoptotic Cell Death in Human Nasopharyngeal Carcinoma Cells through Mitochondrial ROS and TRAIL Pathways.	bufalin	0-7	TNF superfamily member 10	Homo sapiens	107-112
30612454-11	2019	Bufalin increased the pro-apoptotic proteins Bax, and apoptotic associated proteins (cytochrome c, caspase-3, -8 and -9, AIF and Endo G) but reduced anti-apoptotic protein Bcl-2 in NPC-TW 076 cells.	bufalin	0-7	BCL2 associated X, apoptosis regulator	Homo sapiens	45-48
30612454-11	2019	Bufalin increased the pro-apoptotic proteins Bax, and apoptotic associated proteins (cytochrome c, caspase-3, -8 and -9, AIF and Endo G) but reduced anti-apoptotic protein Bcl-2 in NPC-TW 076 cells.	bufalin	0-7	cytochrome c, somatic	Homo sapiens	85-97
30612454-11	2019	Bufalin increased the pro-apoptotic proteins Bax, and apoptotic associated proteins (cytochrome c, caspase-3, -8 and -9, AIF and Endo G) but reduced anti-apoptotic protein Bcl-2 in NPC-TW 076 cells.	bufalin	0-7	caspase 3	Homo sapiens	99-119
30612454-11	2019	Bufalin increased the pro-apoptotic proteins Bax, and apoptotic associated proteins (cytochrome c, caspase-3, -8 and -9, AIF and Endo G) but reduced anti-apoptotic protein Bcl-2 in NPC-TW 076 cells.	bufalin	0-7	apoptosis inducing factor mitochondria associated 1	Homo sapiens	121-124
30612454-11	2019	Bufalin increased the pro-apoptotic proteins Bax, and apoptotic associated proteins (cytochrome c, caspase-3, -8 and -9, AIF and Endo G) but reduced anti-apoptotic protein Bcl-2 in NPC-TW 076 cells.	bufalin	0-7	endonuclease G	Homo sapiens	129-135
30612454-11	2019	Bufalin increased the pro-apoptotic proteins Bax, and apoptotic associated proteins (cytochrome c, caspase-3, -8 and -9, AIF and Endo G) but reduced anti-apoptotic protein Bcl-2 in NPC-TW 076 cells.	bufalin	0-7	BCL2 apoptosis regulator	Homo sapiens	172-177
30612454-13	2019	Based on these findings, we suggest bufalin induced apoptotic cell death via caspase-dependent, mitochondria-dependent and TRAIL pathways in human nasopharyngeal carcinoma NPC-TW 076 cells.	bufalin	36-43	TNF superfamily member 10	Homo sapiens	123-128
30532537-3	2018	Our previous study found that BU can effectively reverse P-glycoprotein (P-gp)-mediated MDR in colon cancer.	bufalin	30-32	ATP binding cassette subfamily B member 1	Homo sapiens	73-77
30532537-3	2018	Our previous study found that BU can effectively reverse P-glycoprotein (P-gp)-mediated MDR in colon cancer.	bufalin	30-32	ATP binding cassette subfamily B member 1	Homo sapiens	57-71
30532537-3	2018	Our previous study found that BU can effectively reverse P-glycoprotein (P-gp)-mediated MDR in colon cancer.	bufalin	30-32	ATP-binding cassette, sub-family B (MDR/TAP), member 1B	Mus musculus	88-91
30532537-10	2018	Results: BU-loaded VES-CSO/TPGS-RGD mixed micelles (BU@VeC/T-RGD MM) were 140.3 nm in diameter with zeta potential of 8.66 mV.	bufalin	9-11	cadherin 5	Mus musculus	55-58
30532537-13	2018	Subsequently, in vivo studies confirmed an enhanced therapeutic efficiency and reduced side effects associated with BU@VeC/T-RGD MM compared with free BU, owing to the enhanced permeation and retention effect, improved pharmacokinetic behavior, and tumor targeting, which lead to MDR-inhibiting effect in LoVo/ADR-bearing nude mice.	bufalin	116-118	cadherin 5	Mus musculus	119-122
30532537-13	2018	Subsequently, in vivo studies confirmed an enhanced therapeutic efficiency and reduced side effects associated with BU@VeC/T-RGD MM compared with free BU, owing to the enhanced permeation and retention effect, improved pharmacokinetic behavior, and tumor targeting, which lead to MDR-inhibiting effect in LoVo/ADR-bearing nude mice.	bufalin	116-118	ATP-binding cassette, sub-family B (MDR/TAP), member 1B	Mus musculus	280-283
30532537-14	2018	Conclusion: Our results demonstrated that VeC/T-RGD MM could be developed as a potential delivery system for BU to improve its antitumor activity against drug-resistant colon cancer.	bufalin	109-111	cadherin 5	Mus musculus	42-45
30166403-0	2018	Role of P53-Senescence Induction in Suppression of LNCaP Prostate Cancer Growth by Cardiotonic Compound Bufalin.	bufalin	104-111	tumor protein p53	Homo sapiens	8-11
30166403-3	2018	In prostate cancer cell models, P53-dependent and independent caspase-mediated apoptosis and androgen receptor (AR) antagonism have been described for bufalin at micromolar concentrations.	bufalin	151-158	tumor protein p53	Homo sapiens	32-35
30166403-3	2018	In prostate cancer cell models, P53-dependent and independent caspase-mediated apoptosis and androgen receptor (AR) antagonism have been described for bufalin at micromolar concentrations.	bufalin	151-158	androgen receptor	Homo sapiens	93-110
30166403-3	2018	In prostate cancer cell models, P53-dependent and independent caspase-mediated apoptosis and androgen receptor (AR) antagonism have been described for bufalin at micromolar concentrations.	bufalin	151-158	androgen receptor	Homo sapiens	112-114
30166403-5	2018	Bufalin exposure induced protein abundance of P53 (not mRNA) and P21CIP1 (CDKN1A), G2 arrest, and increased senescence-like phenotype (SA-galactosidase).	bufalin	0-7	tumor protein p53	Homo sapiens	46-49
30166403-5	2018	Bufalin exposure induced protein abundance of P53 (not mRNA) and P21CIP1 (CDKN1A), G2 arrest, and increased senescence-like phenotype (SA-galactosidase).	bufalin	0-7	cyclin dependent kinase inhibitor 1A	Homo sapiens	65-72
30166403-5	2018	Bufalin exposure induced protein abundance of P53 (not mRNA) and P21CIP1 (CDKN1A), G2 arrest, and increased senescence-like phenotype (SA-galactosidase).	bufalin	0-7	cyclin dependent kinase inhibitor 1A	Homo sapiens	74-80
30166403-6	2018	Small RNAi knocking down of P53 attenuated bufalin-induced senescence, whereas knocking down of P21CIP1 exacerbated bufalin-induced caspase-mediated apoptosis.	bufalin	43-50	tumor protein p53	Homo sapiens	28-31
30166403-6	2018	Small RNAi knocking down of P53 attenuated bufalin-induced senescence, whereas knocking down of P21CIP1 exacerbated bufalin-induced caspase-mediated apoptosis.	bufalin	116-123	cyclin dependent kinase inhibitor 1A	Homo sapiens	96-103
30166403-8	2018	Tumors from bufalin-treated mice exhibited increased phospho-P53 and SA-galactosidase without detectable caspase-mediated apoptosis or suppression of AR and PSA.	bufalin	12-19	transformation related protein 53, pseudogene	Mus musculus	61-64
30166403-8	2018	Tumors from bufalin-treated mice exhibited increased phospho-P53 and SA-galactosidase without detectable caspase-mediated apoptosis or suppression of AR and PSA.	bufalin	12-19	aminopeptidase puromycin sensitive	Homo sapiens	157-160
30166403-9	2018	Our data suggest potential applications of bufalin in therapy of prostate cancer in patients or chemo-interception of prostate precancerous lesions, engaging a selective activation of P53 senescence.	bufalin	43-50	tumor protein p53	Homo sapiens	184-187
30127995-4	2018	This study aimed to investigate the hypothesis that Bufalin induces cell apoptosis and inhibits cell growth in bladder cancer through the inactivation of Na+/K+-ATPase (NKA).	bufalin	52-59	tachykinin precursor 1	Homo sapiens	154-167
30100060-0	2018	Bufalin inhibits cell proliferation and migration of hepatocellular carcinoma cells via APOBEC3F induced intestinal immune network for IgA production signaling pathway.	bufalin	0-7	apolipoprotein B mRNA editing enzyme catalytic subunit 3F	Homo sapiens	88-96
30100060-6	2018	Bufalin inhibits cell proliferation and migration and reduces APOBEC3F expression.	bufalin	0-7	apolipoprotein B mRNA editing enzyme catalytic subunit 3F	Homo sapiens	62-70
30323588-1	2018	Aim: The aim of this study was to prepare transferrin (Tf) and folic acid (FA) co-modified bufalin (BF) liposomes for lung cancer treatment.	bufalin	91-98	transferrin	Mus musculus	42-53
30323588-1	2018	Aim: The aim of this study was to prepare transferrin (Tf) and folic acid (FA) co-modified bufalin (BF) liposomes for lung cancer treatment.	bufalin	100-102	transferrin	Mus musculus	42-53
30127995-4	2018	This study aimed to investigate the hypothesis that Bufalin induces cell apoptosis and inhibits cell growth in bladder cancer through the inactivation of Na+/K+-ATPase (NKA).	bufalin	52-59	tachykinin precursor 1	Homo sapiens	169-172
30127995-6	2018	Subsequently, we found that the expression of NKA was significantly supressed in Bufalin-treated cells and the NKA-alpha3 isoform was most sensitive to Bufalin among all alpha subunits of NKA.	bufalin	81-88	tachykinin precursor 1	Homo sapiens	46-49
30127995-6	2018	Subsequently, we found that the expression of NKA was significantly supressed in Bufalin-treated cells and the NKA-alpha3 isoform was most sensitive to Bufalin among all alpha subunits of NKA.	bufalin	152-159	tachykinin precursor 1	Homo sapiens	46-49
30127995-6	2018	Subsequently, we found that the expression of NKA was significantly supressed in Bufalin-treated cells and the NKA-alpha3 isoform was most sensitive to Bufalin among all alpha subunits of NKA.	bufalin	152-159	tachykinin precursor 1	Homo sapiens	111-114
30127995-6	2018	Subsequently, we found that the expression of NKA was significantly supressed in Bufalin-treated cells and the NKA-alpha3 isoform was most sensitive to Bufalin among all alpha subunits of NKA.	bufalin	152-159	tachykinin precursor 1	Homo sapiens	111-114
30127995-7	2018	By transfection with NKA-alpha3 overexpressing plasmids, the expression of the NKA-alpha3 subunit was upregulated and NKA-alpha3 overexpression was found to markedly attenuated Bufalin-induced cell apoptosis in T24 cells, suggesting NKA-alpha3 played a critical role in Bufalin-induced cell apoptosis.	bufalin	177-184	tachykinin precursor 1	Homo sapiens	21-24
30127995-7	2018	By transfection with NKA-alpha3 overexpressing plasmids, the expression of the NKA-alpha3 subunit was upregulated and NKA-alpha3 overexpression was found to markedly attenuated Bufalin-induced cell apoptosis in T24 cells, suggesting NKA-alpha3 played a critical role in Bufalin-induced cell apoptosis.	bufalin	177-184	tachykinin precursor 1	Homo sapiens	79-82
30127995-7	2018	By transfection with NKA-alpha3 overexpressing plasmids, the expression of the NKA-alpha3 subunit was upregulated and NKA-alpha3 overexpression was found to markedly attenuated Bufalin-induced cell apoptosis in T24 cells, suggesting NKA-alpha3 played a critical role in Bufalin-induced cell apoptosis.	bufalin	177-184	tachykinin precursor 1	Homo sapiens	79-82
30127995-7	2018	By transfection with NKA-alpha3 overexpressing plasmids, the expression of the NKA-alpha3 subunit was upregulated and NKA-alpha3 overexpression was found to markedly attenuated Bufalin-induced cell apoptosis in T24 cells, suggesting NKA-alpha3 played a critical role in Bufalin-induced cell apoptosis.	bufalin	177-184	tachykinin precursor 1	Homo sapiens	79-82
30127995-7	2018	By transfection with NKA-alpha3 overexpressing plasmids, the expression of the NKA-alpha3 subunit was upregulated and NKA-alpha3 overexpression was found to markedly attenuated Bufalin-induced cell apoptosis in T24 cells, suggesting NKA-alpha3 played a critical role in Bufalin-induced cell apoptosis.	bufalin	270-277	tachykinin precursor 1	Homo sapiens	21-24
30127995-7	2018	By transfection with NKA-alpha3 overexpressing plasmids, the expression of the NKA-alpha3 subunit was upregulated and NKA-alpha3 overexpression was found to markedly attenuated Bufalin-induced cell apoptosis in T24 cells, suggesting NKA-alpha3 played a critical role in Bufalin-induced cell apoptosis.	bufalin	270-277	tachykinin precursor 1	Homo sapiens	79-82
30127995-7	2018	By transfection with NKA-alpha3 overexpressing plasmids, the expression of the NKA-alpha3 subunit was upregulated and NKA-alpha3 overexpression was found to markedly attenuated Bufalin-induced cell apoptosis in T24 cells, suggesting NKA-alpha3 played a critical role in Bufalin-induced cell apoptosis.	bufalin	270-277	tachykinin precursor 1	Homo sapiens	79-82
30127995-7	2018	By transfection with NKA-alpha3 overexpressing plasmids, the expression of the NKA-alpha3 subunit was upregulated and NKA-alpha3 overexpression was found to markedly attenuated Bufalin-induced cell apoptosis in T24 cells, suggesting NKA-alpha3 played a critical role in Bufalin-induced cell apoptosis.	bufalin	270-277	tachykinin precursor 1	Homo sapiens	79-82
30094101-0	2018	Bufalin inhibits glycolysis-induced cell growth and proliferation through the suppression of Integrin beta2/FAK signaling pathway in ovarian cancer.	bufalin	0-7	integrin subunit beta 2	Homo sapiens	93-107
30128000-0	2018	Bufalin suppresses the proliferation and metastasis of renal cell carcinoma by inhibiting the PI3K/Akt/mTOR signaling pathway.	bufalin	0-7	AKT serine/threonine kinase 1	Homo sapiens	99-102
30128000-0	2018	Bufalin suppresses the proliferation and metastasis of renal cell carcinoma by inhibiting the PI3K/Akt/mTOR signaling pathway.	bufalin	0-7	mechanistic target of rapamycin kinase	Homo sapiens	103-107
30128000-3	2018	In the present study, it was demonstrated that bufalin inhibited cell proliferation, blocked the cell cycle in the G2/M phase, and reduced the metastasis of human RCC ACHN cells via the upregulation of p21waf/cip1 and E-cadherin and the downregulation of cyclin dependent kinase 1, cyclin B1, N-cadherin, and hypoxia-inducible factor-1alpha (HIF-1alpha).	bufalin	47-54	cyclin dependent kinase inhibitor 1A	Homo sapiens	209-213
30128000-3	2018	In the present study, it was demonstrated that bufalin inhibited cell proliferation, blocked the cell cycle in the G2/M phase, and reduced the metastasis of human RCC ACHN cells via the upregulation of p21waf/cip1 and E-cadherin and the downregulation of cyclin dependent kinase 1, cyclin B1, N-cadherin, and hypoxia-inducible factor-1alpha (HIF-1alpha).	bufalin	47-54	cadherin 1	Homo sapiens	218-228
30128000-3	2018	In the present study, it was demonstrated that bufalin inhibited cell proliferation, blocked the cell cycle in the G2/M phase, and reduced the metastasis of human RCC ACHN cells via the upregulation of p21waf/cip1 and E-cadherin and the downregulation of cyclin dependent kinase 1, cyclin B1, N-cadherin, and hypoxia-inducible factor-1alpha (HIF-1alpha).	bufalin	47-54	cyclin dependent kinase 1	Homo sapiens	255-280
30128000-3	2018	In the present study, it was demonstrated that bufalin inhibited cell proliferation, blocked the cell cycle in the G2/M phase, and reduced the metastasis of human RCC ACHN cells via the upregulation of p21waf/cip1 and E-cadherin and the downregulation of cyclin dependent kinase 1, cyclin B1, N-cadherin, and hypoxia-inducible factor-1alpha (HIF-1alpha).	bufalin	47-54	cyclin B1	Homo sapiens	282-291
30128000-3	2018	In the present study, it was demonstrated that bufalin inhibited cell proliferation, blocked the cell cycle in the G2/M phase, and reduced the metastasis of human RCC ACHN cells via the upregulation of p21waf/cip1 and E-cadherin and the downregulation of cyclin dependent kinase 1, cyclin B1, N-cadherin, and hypoxia-inducible factor-1alpha (HIF-1alpha).	bufalin	47-54	cadherin 2	Homo sapiens	293-303
30128000-3	2018	In the present study, it was demonstrated that bufalin inhibited cell proliferation, blocked the cell cycle in the G2/M phase, and reduced the metastasis of human RCC ACHN cells via the upregulation of p21waf/cip1 and E-cadherin and the downregulation of cyclin dependent kinase 1, cyclin B1, N-cadherin, and hypoxia-inducible factor-1alpha (HIF-1alpha).	bufalin	47-54	hypoxia inducible factor 1 subunit alpha	Homo sapiens	309-340
30128000-3	2018	In the present study, it was demonstrated that bufalin inhibited cell proliferation, blocked the cell cycle in the G2/M phase, and reduced the metastasis of human RCC ACHN cells via the upregulation of p21waf/cip1 and E-cadherin and the downregulation of cyclin dependent kinase 1, cyclin B1, N-cadherin, and hypoxia-inducible factor-1alpha (HIF-1alpha).	bufalin	47-54	hypoxia inducible factor 1 subunit alpha	Homo sapiens	342-352
30128000-4	2018	Further mechanistic study revealed that bufalin reduced the expression of phosphorylated (phospho)-Akt and phospho-mammalian target of rapamycin (mTOR).	bufalin	40-47	AKT serine/threonine kinase 1	Homo sapiens	99-102
30128000-4	2018	Further mechanistic study revealed that bufalin reduced the expression of phosphorylated (phospho)-Akt and phospho-mammalian target of rapamycin (mTOR).	bufalin	40-47	mechanistic target of rapamycin kinase	Homo sapiens	107-144
30128000-4	2018	Further mechanistic study revealed that bufalin reduced the expression of phosphorylated (phospho)-Akt and phospho-mammalian target of rapamycin (mTOR).	bufalin	40-47	mechanistic target of rapamycin kinase	Homo sapiens	146-150
30128000-6	2018	Thus, the present results suggest that bufalin induces cell cycle arrest and suppresses metastasis; this process may be associated with the PI3K/Akt/mTOR signaling pathway.	bufalin	39-46	AKT serine/threonine kinase 1	Homo sapiens	145-148
30128000-6	2018	Thus, the present results suggest that bufalin induces cell cycle arrest and suppresses metastasis; this process may be associated with the PI3K/Akt/mTOR signaling pathway.	bufalin	39-46	mechanistic target of rapamycin kinase	Homo sapiens	149-153
30094101-0	2018	Bufalin inhibits glycolysis-induced cell growth and proliferation through the suppression of Integrin beta2/FAK signaling pathway in ovarian cancer.	bufalin	0-7	protein tyrosine kinase 2	Homo sapiens	108-111
30094101-5	2018	Mechanistically, bufalin exerted its anti-tumor effect by targeting ITGB2/FAK signaling pathway in vitro and in vivo, which could be rescued by the introduction of ITGB2 cDNA in ovarian cancer cells.	bufalin	17-24	integrin subunit beta 2	Homo sapiens	68-73
30094101-5	2018	Mechanistically, bufalin exerted its anti-tumor effect by targeting ITGB2/FAK signaling pathway in vitro and in vivo, which could be rescued by the introduction of ITGB2 cDNA in ovarian cancer cells.	bufalin	17-24	protein tyrosine kinase 2	Homo sapiens	74-77
30094101-5	2018	Mechanistically, bufalin exerted its anti-tumor effect by targeting ITGB2/FAK signaling pathway in vitro and in vivo, which could be rescued by the introduction of ITGB2 cDNA in ovarian cancer cells.	bufalin	17-24	integrin subunit beta 2	Homo sapiens	164-169
30094101-6	2018	These findings provide evidence that bufalin inhibited cellular glycolysis-induced cell growth and proliferation through repression of the ITGB2/FAK pathway, indicating that bufalin may be developed as a chemotherapeutic agent to treat ovarian cancer.	bufalin	37-44	integrin subunit beta 2	Homo sapiens	139-144
30094101-6	2018	These findings provide evidence that bufalin inhibited cellular glycolysis-induced cell growth and proliferation through repression of the ITGB2/FAK pathway, indicating that bufalin may be developed as a chemotherapeutic agent to treat ovarian cancer.	bufalin	37-44	protein tyrosine kinase 2	Homo sapiens	145-148
30094101-6	2018	These findings provide evidence that bufalin inhibited cellular glycolysis-induced cell growth and proliferation through repression of the ITGB2/FAK pathway, indicating that bufalin may be developed as a chemotherapeutic agent to treat ovarian cancer.	bufalin	174-181	integrin subunit beta 2	Homo sapiens	139-144
30094101-6	2018	These findings provide evidence that bufalin inhibited cellular glycolysis-induced cell growth and proliferation through repression of the ITGB2/FAK pathway, indicating that bufalin may be developed as a chemotherapeutic agent to treat ovarian cancer.	bufalin	174-181	protein tyrosine kinase 2	Homo sapiens	145-148
29805658-0	2018	Bufalin induces apoptosis in human esophageal carcinoma ECA109 cells by inhibiting the activation of the mTOR/p70S6K pathway.	bufalin	0-7	mechanistic target of rapamycin kinase	Homo sapiens	105-109
29653366-9	2018	The results indicated that Bufalin inhibited the expression of ATP1A1 in glioblastoma by promoting the activation of proteasomes and the subsequent protein degradation of ATP1A1, while Bufalin had no effect on ATP1A1 protein synthesis.	bufalin	27-34	ATPase Na+/K+ transporting subunit alpha 1	Homo sapiens	63-69
29653366-9	2018	The results indicated that Bufalin inhibited the expression of ATP1A1 in glioblastoma by promoting the activation of proteasomes and the subsequent protein degradation of ATP1A1, while Bufalin had no effect on ATP1A1 protein synthesis.	bufalin	27-34	ATPase Na+/K+ transporting subunit alpha 1	Homo sapiens	171-177
29653366-9	2018	The results indicated that Bufalin inhibited the expression of ATP1A1 in glioblastoma by promoting the activation of proteasomes and the subsequent protein degradation of ATP1A1, while Bufalin had no effect on ATP1A1 protein synthesis.	bufalin	27-34	ATPase Na+/K+ transporting subunit alpha 1	Homo sapiens	171-177
29653366-10	2018	Bufalin also inhibited the expression of ATP1A1 in xenografted mice and significantly suppressed tumor growth.	bufalin	0-7	ATPase, Na+/K+ transporting, alpha 1 polypeptide	Mus musculus	41-47
29653366-12	2018	In conclusion, Bufalin significantly inhibited the expression of ATP1A1 in glioblastoma cells by activating the ubiquitin-proteasome signaling pathway.	bufalin	15-22	ATPase Na+/K+ transporting subunit alpha 1	Homo sapiens	65-71
29620259-0	2018	Synergistic anti-hepatoma effect of bufalin combined with sorafenib via mediating the tumor vascular microenvironment by targeting mTOR/VEGF signaling.	bufalin	36-43	mechanistic target of rapamycin kinase	Homo sapiens	131-135
29620259-0	2018	Synergistic anti-hepatoma effect of bufalin combined with sorafenib via mediating the tumor vascular microenvironment by targeting mTOR/VEGF signaling.	bufalin	36-43	vascular endothelial growth factor A	Homo sapiens	136-140
29620259-13	2018	In conclusion, the results revealed a synergistic anti-hepatoma effect of bufalin combined with sorafenib via affecting the tumor vascular microenvironment by targeting mTOR/VEGF signaling.	bufalin	74-81	mechanistic target of rapamycin kinase	Homo sapiens	169-173
29620259-13	2018	In conclusion, the results revealed a synergistic anti-hepatoma effect of bufalin combined with sorafenib via affecting the tumor vascular microenvironment by targeting mTOR/VEGF signaling.	bufalin	74-81	vascular endothelial growth factor A	Homo sapiens	174-178
29636364-2	2018	In this context, therefore, bufalin was employed as a "tool compound" to evaluate inhibitors of SRC in alternative endometriosis treatment.	bufalin	28-35	SRC proto-oncogene, non-receptor tyrosine kinase	Homo sapiens	96-99
29636364-5	2018	In stromal cells of endometriotic lesions, bufalin treatment increased the levels of pyroptosis markers (caspase 1 and the active form of interleukin 1beta) and reduced proliferation.	bufalin	43-50	caspase 1	Homo sapiens	105-114
29636364-5	2018	In stromal cells of endometriotic lesions, bufalin treatment increased the levels of pyroptosis markers (caspase 1 and the active form of interleukin 1beta) and reduced proliferation.	bufalin	43-50	interleukin 1 beta	Homo sapiens	138-155
29636364-7	2018	Collectively, the bufalin-induced disruption of the SRC-1 isoform/ERbeta axis might induce apoptosis, pyroptosis and ERS signaling in endometriotic lesions, causing the suppression of endometriosis.	bufalin	18-25	SRC proto-oncogene, non-receptor tyrosine kinase	Homo sapiens	52-57
29653366-5	2018	The effect of ATP1A1 on the sensitivity of glioblastoma cells to Bufalin was investigated using MTT assays, RT-PCR and siRNA.	bufalin	65-72	ATPase Na+/K+ transporting subunit alpha 1	Homo sapiens	14-20
29693132-4	2018	It was revealed that either bufalin or sorafenib suppressed PLC/PRF/5 and SMMC-7721 cell proliferation in a concentration-dependent manner following incubation for 24 h, and the inhibitory effect was augmented with their combined treatment.	bufalin	28-35	heparan sulfate proteoglycan 2	Homo sapiens	60-63
29805658-0	2018	Bufalin induces apoptosis in human esophageal carcinoma ECA109 cells by inhibiting the activation of the mTOR/p70S6K pathway.	bufalin	0-7	ribosomal protein S6 kinase B1	Homo sapiens	110-116
29805658-1	2018	The present study examined whether bufalin could induce human esophageal carcinoma ECA109 cells apoptosis via inhibiting the activation of mechanistic target of rapamycin (mTOR)/p70 S6 kinase (p70S6K) pathway is discussed in this article.	bufalin	35-42	mechanistic target of rapamycin kinase	Homo sapiens	139-170
29805658-1	2018	The present study examined whether bufalin could induce human esophageal carcinoma ECA109 cells apoptosis via inhibiting the activation of mechanistic target of rapamycin (mTOR)/p70 S6 kinase (p70S6K) pathway is discussed in this article.	bufalin	35-42	mechanistic target of rapamycin kinase	Homo sapiens	172-176
29805658-1	2018	The present study examined whether bufalin could induce human esophageal carcinoma ECA109 cells apoptosis via inhibiting the activation of mechanistic target of rapamycin (mTOR)/p70 S6 kinase (p70S6K) pathway is discussed in this article.	bufalin	35-42	ribosomal protein S6 kinase B1	Homo sapiens	193-199
29805658-2	2018	The present study used the esophageal squamous cell carcinoma ECA109 cell line to assess the apoptosis-inducing effects of bufalin via inhibition of the mTOR/p70S6K pathways.	bufalin	123-130	mechanistic target of rapamycin kinase	Homo sapiens	153-157
29805658-2	2018	The present study used the esophageal squamous cell carcinoma ECA109 cell line to assess the apoptosis-inducing effects of bufalin via inhibition of the mTOR/p70S6K pathways.	bufalin	123-130	ribosomal protein S6 kinase B1	Homo sapiens	158-164
29805658-7	2018	The levels of p-p70S6K and cIAP-1 were significantly higher in the wtmTOR-transfected group than in the control and empty vector-transfected groups, and then reduced following addition of bufalin; however, BAD expression was significantly lower in the wtmTOR-transfected group.	bufalin	188-195	ribosomal protein S6 kinase B1	Homo sapiens	16-22
29805658-7	2018	The levels of p-p70S6K and cIAP-1 were significantly higher in the wtmTOR-transfected group than in the control and empty vector-transfected groups, and then reduced following addition of bufalin; however, BAD expression was significantly lower in the wtmTOR-transfected group.	bufalin	188-195	baculoviral IAP repeat containing 2	Homo sapiens	27-33
29805658-9	2018	In conclusion, the findings of the present study demonstrated that bufalin induced apoptosis in esophageal carcinoma cells via the inhibition of the mTOR/p70S6K pathway and indicated that treatment with bufalin could be combined with chemotherapy to overcome the resistance of esophageal carcinoma cells to chemotherapeutic-induced apoptosis.	bufalin	67-74	mechanistic target of rapamycin kinase	Homo sapiens	149-153
29805658-9	2018	In conclusion, the findings of the present study demonstrated that bufalin induced apoptosis in esophageal carcinoma cells via the inhibition of the mTOR/p70S6K pathway and indicated that treatment with bufalin could be combined with chemotherapy to overcome the resistance of esophageal carcinoma cells to chemotherapeutic-induced apoptosis.	bufalin	67-74	ribosomal protein S6 kinase B1	Homo sapiens	154-160
29805658-9	2018	In conclusion, the findings of the present study demonstrated that bufalin induced apoptosis in esophageal carcinoma cells via the inhibition of the mTOR/p70S6K pathway and indicated that treatment with bufalin could be combined with chemotherapy to overcome the resistance of esophageal carcinoma cells to chemotherapeutic-induced apoptosis.	bufalin	203-210	ribosomal protein S6 kinase B1	Homo sapiens	154-160
29546393-0	2018	Bufalin inhibits human breast cancer tumorigenesis by inducing cell death through the ROS-mediated RIP1/RIP3/PARP-1 pathways.	bufalin	0-7	receptor interacting serine/threonine kinase 1	Homo sapiens	99-103
29658591-8	2018	When combined with autophagy inhibitors 3-MA and/or CQ for 12 h, bufalin significantly inhibited the expression of LC3-I and Beclin-1 in HCC-LM3 cells, and upregulated the expression of LC3-II and P62 in HCC-LM3 cells.	bufalin	65-72	beclin 1	Homo sapiens	125-133
29658591-8	2018	When combined with autophagy inhibitors 3-MA and/or CQ for 12 h, bufalin significantly inhibited the expression of LC3-I and Beclin-1 in HCC-LM3 cells, and upregulated the expression of LC3-II and P62 in HCC-LM3 cells.	bufalin	65-72	nucleoporin 62	Homo sapiens	197-200
29658591-9	2018	When combined with autophagy inhibitors 3-MA and/or CQ for 24 h, bufalin significantly inhibited the LC3-II expression in HCC-LM3 cells, and upregulated the LC3-I, P62 and Beclin-1 expression in HCC-LM3 cells.	bufalin	65-72	nucleoporin 62	Homo sapiens	164-167
29658591-9	2018	When combined with autophagy inhibitors 3-MA and/or CQ for 24 h, bufalin significantly inhibited the LC3-II expression in HCC-LM3 cells, and upregulated the LC3-I, P62 and Beclin-1 expression in HCC-LM3 cells.	bufalin	65-72	beclin 1	Homo sapiens	172-180
29658591-10	2018	When combined with autophagy inhibitors 3-MA and/or CQ for 48 h, bufalin significantly inhibited the expression of LC3-II and Beclin-1 in HCC-LM3 cells, and upregulated the expression of LC3-I and P62 in HCC-LM3 cells.	bufalin	65-72	beclin 1	Homo sapiens	126-134
29658591-10	2018	When combined with autophagy inhibitors 3-MA and/or CQ for 48 h, bufalin significantly inhibited the expression of LC3-II and Beclin-1 in HCC-LM3 cells, and upregulated the expression of LC3-I and P62 in HCC-LM3 cells.	bufalin	65-72	nucleoporin 62	Homo sapiens	197-200
29546393-0	2018	Bufalin inhibits human breast cancer tumorigenesis by inducing cell death through the ROS-mediated RIP1/RIP3/PARP-1 pathways.	bufalin	0-7	receptor interacting serine/threonine kinase 3	Homo sapiens	104-108
29546393-0	2018	Bufalin inhibits human breast cancer tumorigenesis by inducing cell death through the ROS-mediated RIP1/RIP3/PARP-1 pathways.	bufalin	0-7	poly(ADP-ribose) polymerase 1	Homo sapiens	109-115
29546393-4	2018	Bufalin-induced cell death was prevented by small molecule inhibitors of RIP1 and poly (ADP-ribose) polymerase-1 (PARP-1) or genetic knockdown of RIP3 by shRNA transfection.	bufalin	0-7	receptor interacting serine/threonine kinase 1	Homo sapiens	73-77
29546393-4	2018	Bufalin-induced cell death was prevented by small molecule inhibitors of RIP1 and poly (ADP-ribose) polymerase-1 (PARP-1) or genetic knockdown of RIP3 by shRNA transfection.	bufalin	0-7	poly(ADP-ribose) polymerase 1	Homo sapiens	82-112
29546393-4	2018	Bufalin-induced cell death was prevented by small molecule inhibitors of RIP1 and poly (ADP-ribose) polymerase-1 (PARP-1) or genetic knockdown of RIP3 by shRNA transfection.	bufalin	0-7	poly(ADP-ribose) polymerase 1	Homo sapiens	114-120
29546393-4	2018	Bufalin-induced cell death was prevented by small molecule inhibitors of RIP1 and poly (ADP-ribose) polymerase-1 (PARP-1) or genetic knockdown of RIP3 by shRNA transfection.	bufalin	0-7	receptor interacting serine/threonine kinase 3	Homo sapiens	146-150
29546393-6	2018	We also found that bufalin increased intracellular reactive oxygen species levels; and cell death by bufalin was inhibited by the antioxidant NAC.	bufalin	19-26	X-linked Kx blood group	Homo sapiens	142-145
29721392-8	2018	Our data suggest that bufalin can suppress the metastasis of HCC cells in poly (I:C) therapy by impairing TBK1 activation, indicating that bufalin may be used in combination with poly (I:C) therapy in HCC treatment for the sake of reversing poly (I:C)-triggered metastasis of HCC cells.	bufalin	22-29	TANK binding kinase 1	Homo sapiens	106-110
29616114-9	2018	Furthermore, treatment with bufalin abolished HIF-1alpha expression under hypoxia, suggesting that bufalin exerts cytotoxicity under hypoxia by regulating HIF-1alpha.	bufalin	28-35	hypoxia inducible factor 1 subunit alpha	Homo sapiens	46-56
29616114-9	2018	Furthermore, treatment with bufalin abolished HIF-1alpha expression under hypoxia, suggesting that bufalin exerts cytotoxicity under hypoxia by regulating HIF-1alpha.	bufalin	28-35	hypoxia inducible factor 1 subunit alpha	Homo sapiens	155-165
29616114-9	2018	Furthermore, treatment with bufalin abolished HIF-1alpha expression under hypoxia, suggesting that bufalin exerts cytotoxicity under hypoxia by regulating HIF-1alpha.	bufalin	99-106	hypoxia inducible factor 1 subunit alpha	Homo sapiens	46-56
29616114-9	2018	Furthermore, treatment with bufalin abolished HIF-1alpha expression under hypoxia, suggesting that bufalin exerts cytotoxicity under hypoxia by regulating HIF-1alpha.	bufalin	99-106	hypoxia inducible factor 1 subunit alpha	Homo sapiens	155-165
29616114-10	2018	These results indicate that bufalin induces apoptosis in MM cells through DSB under hypoxic conditions by inhibiting HIF-1alpha, suggesting that bufalin could be useful for eradication of drug-resistant MM cells in the hypoxic microenvironment.	bufalin	28-35	hypoxia inducible factor 1 subunit alpha	Homo sapiens	117-127
29616114-10	2018	These results indicate that bufalin induces apoptosis in MM cells through DSB under hypoxic conditions by inhibiting HIF-1alpha, suggesting that bufalin could be useful for eradication of drug-resistant MM cells in the hypoxic microenvironment.	bufalin	145-152	hypoxia inducible factor 1 subunit alpha	Homo sapiens	117-127
29849804-8	2018	Additionally, bufalin combined with 5-FU reduced the expression levels of anti-apoptotic proteins, such as Mcl-1, XIAP and Bcl-2 and upregulated the levels of the pro-apoptotic proteins, Bax and Bad.	bufalin	14-21	MCL1 apoptosis regulator, BCL2 family member	Homo sapiens	107-112
29849804-8	2018	Additionally, bufalin combined with 5-FU reduced the expression levels of anti-apoptotic proteins, such as Mcl-1, XIAP and Bcl-2 and upregulated the levels of the pro-apoptotic proteins, Bax and Bad.	bufalin	14-21	X-linked inhibitor of apoptosis	Homo sapiens	114-118
29849804-8	2018	Additionally, bufalin combined with 5-FU reduced the expression levels of anti-apoptotic proteins, such as Mcl-1, XIAP and Bcl-2 and upregulated the levels of the pro-apoptotic proteins, Bax and Bad.	bufalin	14-21	BCL2 apoptosis regulator	Homo sapiens	123-128
29849804-8	2018	Additionally, bufalin combined with 5-FU reduced the expression levels of anti-apoptotic proteins, such as Mcl-1, XIAP and Bcl-2 and upregulated the levels of the pro-apoptotic proteins, Bax and Bad.	bufalin	14-21	BCL2 associated X, apoptosis regulator	Homo sapiens	187-190
29849804-10	2018	It was determined that the synergistic effect between 5-FU and bufalin was diminished following the silencing of Bax.	bufalin	63-70	BCL2 associated X, apoptosis regulator	Homo sapiens	113-116
29622868-0	2018	Bufalin Enhances the Cytotoxity of Human Multiple Myeloma Cells H929 to AKT Inhibitor MK2206: The Role of Protein AKT Phosphorylation.	bufalin	0-7	AKT serine/threonine kinase 1	Homo sapiens	72-75
29622868-0	2018	Bufalin Enhances the Cytotoxity of Human Multiple Myeloma Cells H929 to AKT Inhibitor MK2206: The Role of Protein AKT Phosphorylation.	bufalin	0-7	AKT serine/threonine kinase 1	Homo sapiens	114-117
29622868-3	2018	The apoptotic cells markedly increased after treated with nM bufalin and muM MK2206, including caspase3 and PARP1 proteins activated.	bufalin	61-68	caspase 3	Homo sapiens	95-103
29622868-3	2018	The apoptotic cells markedly increased after treated with nM bufalin and muM MK2206, including caspase3 and PARP1 proteins activated.	bufalin	61-68	poly(ADP-ribose) polymerase 1	Homo sapiens	108-113
29497076-0	2018	Bufalin suppresses hepatocarcinogenesis by targeting beta-catenin/TCF signaling via cell cycle-related kinase.	bufalin	0-7	catenin beta 1	Homo sapiens	53-65
29497076-0	2018	Bufalin suppresses hepatocarcinogenesis by targeting beta-catenin/TCF signaling via cell cycle-related kinase.	bufalin	0-7	hepatocyte nuclear factor 4 alpha	Homo sapiens	66-69
29546393-6	2018	We also found that bufalin increased intracellular reactive oxygen species levels; and cell death by bufalin was inhibited by the antioxidant NAC.	bufalin	101-108	X-linked Kx blood group	Homo sapiens	142-145
29546393-7	2018	In a mouse xenograft model of human breast cancer, bufalin induced PARP-1-dependent tumor cell death and inhibited tumor growth.	bufalin	51-58	poly(ADP-ribose) polymerase 1	Homo sapiens	67-73
29497076-0	2018	Bufalin suppresses hepatocarcinogenesis by targeting beta-catenin/TCF signaling via cell cycle-related kinase.	bufalin	0-7	cyclin dependent kinase 20	Homo sapiens	84-109
29497076-8	2018	We revealed that bufalin suppresses PLC5 HCC cell proliferation, transformation and cell cycle progression rather than LO2 cells, which is correlated with CCRK-mediated beta-catenin/TCF signaling.	bufalin	17-24	cyclin dependent kinase 20	Homo sapiens	155-159
29497076-8	2018	We revealed that bufalin suppresses PLC5 HCC cell proliferation, transformation and cell cycle progression rather than LO2 cells, which is correlated with CCRK-mediated beta-catenin/TCF signaling.	bufalin	17-24	catenin beta 1	Homo sapiens	169-181
29497076-8	2018	We revealed that bufalin suppresses PLC5 HCC cell proliferation, transformation and cell cycle progression rather than LO2 cells, which is correlated with CCRK-mediated beta-catenin/TCF signaling.	bufalin	17-24	hepatocyte nuclear factor 4 alpha	Homo sapiens	182-185
29497076-10	2018	Thus, bufalin is a potential anti-HCC therapeutic candidate through the inhibition of CCRK-driven beta-catenin/TCF oncogenic signaling pathway.	bufalin	6-13	cyclin dependent kinase 20	Homo sapiens	86-90
29497076-10	2018	Thus, bufalin is a potential anti-HCC therapeutic candidate through the inhibition of CCRK-driven beta-catenin/TCF oncogenic signaling pathway.	bufalin	6-13	catenin beta 1	Homo sapiens	98-110
29497076-10	2018	Thus, bufalin is a potential anti-HCC therapeutic candidate through the inhibition of CCRK-driven beta-catenin/TCF oncogenic signaling pathway.	bufalin	6-13	hepatocyte nuclear factor 4 alpha	Homo sapiens	111-114
29568394-3	2018	Here, we reported for the first time that cardiac glycoside bufalin induced mitotic entry delay and prometaphase arrest by inhibition of activation of Aurora A/B.	bufalin	60-67	aurora kinase A	Homo sapiens	151-161
29568394-4	2018	Furthermore, cardiac glycoside bufalin prevented Aurora A recruitment to mitotic centrosomes and Aurora B recruitment to unattached kinetochores.	bufalin	31-38	aurora kinase A	Homo sapiens	49-57
29568394-8	2018	Taken together, cardiac glycoside bufalin induces mitotic entry delay and mitotic arrest in cancer cells through inhibition of Aurora A/B activation via PI3K-Akt pathway.	bufalin	34-41	aurora kinase A	Homo sapiens	127-135
29568394-8	2018	Taken together, cardiac glycoside bufalin induces mitotic entry delay and mitotic arrest in cancer cells through inhibition of Aurora A/B activation via PI3K-Akt pathway.	bufalin	34-41	AKT serine/threonine kinase 1	Homo sapiens	158-161
29721392-8	2018	Our data suggest that bufalin can suppress the metastasis of HCC cells in poly (I:C) therapy by impairing TBK1 activation, indicating that bufalin may be used in combination with poly (I:C) therapy in HCC treatment for the sake of reversing poly (I:C)-triggered metastasis of HCC cells.	bufalin	139-146	TANK binding kinase 1	Homo sapiens	106-110
29805736-0	2018	Bufalin inhibits gastric cancer invasion and metastasis by down-regulating Wnt/ASCL2 expression.	bufalin	0-7	achaete-scute family bHLH transcription factor 2	Mus musculus	79-84
29618300-6	2018	We found that CTS toxicity is not directly related to the degree of Na+,K+-ATPase inhibition, and that bufalin and digoxin, like ouabain, are capable of activating ERK1/2 and p38, but with different concentration and time profiles.	bufalin	103-110	mitogen activated protein kinase 3	Rattus norvegicus	164-170
29618300-6	2018	We found that CTS toxicity is not directly related to the degree of Na+,K+-ATPase inhibition, and that bufalin and digoxin, like ouabain, are capable of activating ERK1/2 and p38, but with different concentration and time profiles.	bufalin	103-110	mitogen activated protein kinase 14	Rattus norvegicus	175-178
29805736-5	2018	Furthermore, bufalin inhibited ASCL2 expression and down-regulated the expression of invasion-related genes such as MMP2, MMP9, and vimentin, thereby suppressing epithelial-mesenchymal transition (EMT) in gastric cancer.	bufalin	13-20	achaete-scute family bHLH transcription factor 2	Mus musculus	31-36
29805736-5	2018	Furthermore, bufalin inhibited ASCL2 expression and down-regulated the expression of invasion-related genes such as MMP2, MMP9, and vimentin, thereby suppressing epithelial-mesenchymal transition (EMT) in gastric cancer.	bufalin	13-20	matrix metallopeptidase 2	Mus musculus	116-120
29805736-5	2018	Furthermore, bufalin inhibited ASCL2 expression and down-regulated the expression of invasion-related genes such as MMP2, MMP9, and vimentin, thereby suppressing epithelial-mesenchymal transition (EMT) in gastric cancer.	bufalin	13-20	matrix metallopeptidase 9	Mus musculus	122-126
29805736-5	2018	Furthermore, bufalin inhibited ASCL2 expression and down-regulated the expression of invasion-related genes such as MMP2, MMP9, and vimentin, thereby suppressing epithelial-mesenchymal transition (EMT) in gastric cancer.	bufalin	13-20	vimentin	Mus musculus	132-140
28983595-0	2017	Bufalin induced apoptosis in SCC-4 human tongue cancer cells by decreasing Bcl-2 and increasing Bax expression via the mitochondria-dependent pathway.	bufalin	0-7	BCL2 apoptosis regulator	Homo sapiens	75-80
28755515-0	2017	Bufalin, a bufanolide steroid from the parotoid glands of the Chinese toad, suppresses hERG K+ currents expressed in HEK293 cells.	bufalin	0-7	ETS transcription factor ERG	Homo sapiens	87-91
28755515-1	2017	In this study, we investigated the effect of bufalin on the human ether-a-go-go-related gene (hERG) K+ channels using the perforated patch recording technique.	bufalin	45-52	ETS transcription factor ERG	Homo sapiens	94-98
28755515-3	2017	These findings suggest that bufalin is a potent hERG K+ channel blocker and may provide a new way for understanding Chan Su-induced arrhythmia.	bufalin	28-35	ETS transcription factor ERG	Homo sapiens	48-52
28983595-0	2017	Bufalin induced apoptosis in SCC-4 human tongue cancer cells by decreasing Bcl-2 and increasing Bax expression via the mitochondria-dependent pathway.	bufalin	0-7	BCL2 associated X, apoptosis regulator	Homo sapiens	96-99
28983595-1	2017	The aim of the present study was to investigate the cytotoxic effects of bufalin on SCC-4 human tongue cancer cells.	bufalin	73-80	MAU2 sister chromatid cohesion factor	Homo sapiens	84-89
28983595-5	2017	Levels of reactive oxygen species (ROS), Ca2+, nitric oxide (NO) and mitochondrial membrane potential (DeltaPsim) were measured by flow cytometry, and bufalin was observed to increase Ca2+ and NO production, decrease the DeltaPsim and reduce ROS production in SCC-4 cells.	bufalin	151-158	MAU2 sister chromatid cohesion factor	Homo sapiens	260-265
28983595-9	2017	Based on these findings, bufalin may induce apoptotic cell death via mitochondria-dependent pathways in human tongue cancer SCC-4 cells.	bufalin	25-32	MAU2 sister chromatid cohesion factor	Homo sapiens	124-129
28840279-10	2017	Bufalin upregulated OPG and downregulated RANKL.	bufalin	0-7	tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)	Mus musculus	20-23
28840279-10	2017	Bufalin upregulated OPG and downregulated RANKL.	bufalin	0-7	tumor necrosis factor (ligand) superfamily, member 11	Mus musculus	42-47
28840279-11	2017	The CB2 receptor inverse agonist, AM630, reduced the pain relief of bufalin treatment in the mouse bone cancer model.	bufalin	68-75	cannabinoid receptor 2 (macrophage)	Mus musculus	4-7
28656204-0	2017	Effects of bufalin on the mTOR/p70S6K pathway and apoptosis in esophageal squamous cell carcinoma in nude mice.	bufalin	11-18	mechanistic target of rapamycin kinase	Mus musculus	26-30
28983595-0	2017	Bufalin induced apoptosis in SCC-4 human tongue cancer cells by decreasing Bcl-2 and increasing Bax expression via the mitochondria-dependent pathway.	bufalin	0-7	MAU2 sister chromatid cohesion factor	Homo sapiens	29-34
28838965-11	2017	The mechanism responsible for bufalin-induced apoptosis was the formation of ER stress via the IRE1-JNK pathway.	bufalin	30-37	endoplasmic reticulum to nucleus signaling 1	Homo sapiens	95-99
28838965-11	2017	The mechanism responsible for bufalin-induced apoptosis was the formation of ER stress via the IRE1-JNK pathway.	bufalin	30-37	mitogen-activated protein kinase 8	Homo sapiens	100-103
28656204-1	2017	The aim of this study was to investigate the effects of bufalin on the mammalian target of rapamycin (mTOR/p70S6 kinase (p70S6K) signaling pathway and cell apoptosis in orthotopically transplanted tumors in nude mice.	bufalin	56-63	mechanistic target of rapamycin kinase	Homo sapiens	71-100
28656204-1	2017	The aim of this study was to investigate the effects of bufalin on the mammalian target of rapamycin (mTOR/p70S6 kinase (p70S6K) signaling pathway and cell apoptosis in orthotopically transplanted tumors in nude mice.	bufalin	56-63	mechanistic target of rapamycin kinase	Homo sapiens	102-106
28656204-1	2017	The aim of this study was to investigate the effects of bufalin on the mammalian target of rapamycin (mTOR/p70S6 kinase (p70S6K) signaling pathway and cell apoptosis in orthotopically transplanted tumors in nude mice.	bufalin	56-63	ribosomal protein S6 kinase B1	Homo sapiens	121-127
28656204-7	2017	This was shown by the decrease in the expression of mTOR, p70S6K and 4EBP1, which suggested that bufalin may possibly be used to inhibit tumor growth via the inhibition of the activation of p70S6K and 4EBP1.	bufalin	97-104	mechanistic target of rapamycin kinase	Mus musculus	52-56
28656204-7	2017	This was shown by the decrease in the expression of mTOR, p70S6K and 4EBP1, which suggested that bufalin may possibly be used to inhibit tumor growth via the inhibition of the activation of p70S6K and 4EBP1.	bufalin	97-104	ribosomal protein S6 kinase, polypeptide 1	Mus musculus	58-74
28656204-7	2017	This was shown by the decrease in the expression of mTOR, p70S6K and 4EBP1, which suggested that bufalin may possibly be used to inhibit tumor growth via the inhibition of the activation of p70S6K and 4EBP1.	bufalin	97-104	ribosomal protein S6 kinase, polypeptide 1	Mus musculus	190-206
28624793-3	2017	The purpose of this present study was to investigate the reversal effect of BU on ABCB1-mediated multidrug resistance in colorectal cancer.	bufalin	76-78	ATP binding cassette subfamily B member 1	Homo sapiens	82-87
28693242-9	2017	Therefore, bufalin may provide an effective therapeutic strategy for the safe treatment of human bladder cancers that are resistant to TRAIL.	bufalin	11-18	TNF superfamily member 10	Homo sapiens	135-140
28693242-0	2017	Bufalin sensitizes human bladder carcinoma cells to TRAIL-mediated apoptosis.	bufalin	0-7	TNF superfamily member 10	Homo sapiens	52-57
28693242-7	2017	Furthermore, the data revealed that bufalin and TRAIL activated caspase-3, -8 and -9 and subsequently increased the degradation of poly (ADP-ribose) polymerase.	bufalin	36-43	caspase 3	Homo sapiens	64-84
28693242-7	2017	Furthermore, the data revealed that bufalin and TRAIL activated caspase-3, -8 and -9 and subsequently increased the degradation of poly (ADP-ribose) polymerase.	bufalin	36-43	poly(ADP-ribose) polymerase 1	Homo sapiens	131-159
28624793-4	2017	BU at safe concentration (5, 10, 20 nM) could reverse chemosensitivity of ABCB1-overexpression HCT8/ADR, LoVo/ADR and HCT8/ABCB1 nearly back to their parental cells level.	bufalin	0-2	ATP binding cassette subfamily B member 1	Homo sapiens	74-79
28624793-4	2017	BU at safe concentration (5, 10, 20 nM) could reverse chemosensitivity of ABCB1-overexpression HCT8/ADR, LoVo/ADR and HCT8/ABCB1 nearly back to their parental cells level.	bufalin	0-2	ATP binding cassette subfamily B member 1	Homo sapiens	123-128
28624793-6	2017	Furthermore, Western blot assays showed that BU significantly inhibited the expression level of ABCB1 protein.	bufalin	45-47	ATP binding cassette subfamily B member 1	Homo sapiens	96-101
28624793-7	2017	Meanwhile, BU stimulated the ATPase activity of ABCB1, which suggested that BU might be a substrate of ABCB1.	bufalin	11-13	ATP binding cassette subfamily B member 1	Homo sapiens	48-53
28624793-7	2017	Meanwhile, BU stimulated the ATPase activity of ABCB1, which suggested that BU might be a substrate of ABCB1.	bufalin	11-13	ATP binding cassette subfamily B member 1	Homo sapiens	103-108
28624793-7	2017	Meanwhile, BU stimulated the ATPase activity of ABCB1, which suggested that BU might be a substrate of ABCB1.	bufalin	76-78	ATP binding cassette subfamily B member 1	Homo sapiens	48-53
28624793-7	2017	Meanwhile, BU stimulated the ATPase activity of ABCB1, which suggested that BU might be a substrate of ABCB1.	bufalin	76-78	ATP binding cassette subfamily B member 1	Homo sapiens	103-108
28624793-8	2017	More interestingly, docking analysis predicted that BU could be docked into the large hydrophobic drug-binding cavity of human ABCB1.	bufalin	52-54	ATP binding cassette subfamily B member 1	Homo sapiens	127-132
28364352-1	2017	OBJECTIVE: To explore the effects of bufalin on inhibiting proliferation, up-regulating methylation of Wilm" tumor 1 gene (WT1) as well as its possible mechanisms in human erythroid leukemic (HEL) cells.	bufalin	37-44	WT1 transcription factor	Homo sapiens	123-126
28492559-0	2017	MK2206 enhances the cytocidal effects of bufalin in multiple myeloma by inhibiting the AKT/mTOR pathway.	bufalin	41-48	AKT serine/threonine kinase 1	Homo sapiens	87-90
28492559-0	2017	MK2206 enhances the cytocidal effects of bufalin in multiple myeloma by inhibiting the AKT/mTOR pathway.	bufalin	41-48	mechanistic target of rapamycin kinase	Homo sapiens	91-95
28492559-3	2017	Previous studies conducted by our group demonstrated that bufalin activated the AKT/mTOR pathway in myeloma cells, which is considered an essential pathway to disease progression and is related to drug resistance in MM.	bufalin	58-65	AKT serine/threonine kinase 1	Homo sapiens	80-83
28492559-3	2017	Previous studies conducted by our group demonstrated that bufalin activated the AKT/mTOR pathway in myeloma cells, which is considered an essential pathway to disease progression and is related to drug resistance in MM.	bufalin	58-65	mechanistic target of rapamycin kinase	Homo sapiens	84-88
28492559-4	2017	In view of the significant role of AKT in MM, the allosteric AKT inhibitor MK2206 was selected in order to enhance the antitumor effects of bufalin in different MM cell lines (NCI-H929, U266, LP-1 and RPMI8226).	bufalin	140-147	AKT serine/threonine kinase 1	Homo sapiens	61-64
28492559-11	2017	In conclusion, the data suggested that MK2206 significantly enhanced the cytocidal effects of bufalin in MM cells, regardless of the sensitivity to bortezomib, via the inhibition of the AKT/mTOR pathway.	bufalin	94-101	AKT serine/threonine kinase 1	Homo sapiens	186-189
28492559-11	2017	In conclusion, the data suggested that MK2206 significantly enhanced the cytocidal effects of bufalin in MM cells, regardless of the sensitivity to bortezomib, via the inhibition of the AKT/mTOR pathway.	bufalin	94-101	mechanistic target of rapamycin kinase	Homo sapiens	190-194
28364352-8	2017	While, the expression levels of DNMT3a and DNMT3b protein gradually increased by bufalin treatment in a dose-dependent manner.	bufalin	81-88	DNA methyltransferase 3 alpha	Homo sapiens	32-38
28364352-8	2017	While, the expression levels of DNMT3a and DNMT3b protein gradually increased by bufalin treatment in a dose-dependent manner.	bufalin	81-88	DNA methyltransferase 3 beta	Homo sapiens	43-49
28364352-9	2017	CONCLUSIONS: Bufalin can not only significantly inhibit the proliferation of HEL cells and arrest cell cycle at G0/G1 phase, but also induce cellular apoptosis and down-regulate the expression level of WT1.	bufalin	13-20	WT1 transcription factor	Homo sapiens	202-205
28364352-10	2017	Our results provide the evidence of bufalin for anti-leukemia, its mechanism may involve in increasing WT1 methylation status which is related to the up-regulation of DNMT3a and DNMT3b proteins in erythroid leukemic HEL cells.	bufalin	36-43	WT1 transcription factor	Homo sapiens	103-106
28364352-10	2017	Our results provide the evidence of bufalin for anti-leukemia, its mechanism may involve in increasing WT1 methylation status which is related to the up-regulation of DNMT3a and DNMT3b proteins in erythroid leukemic HEL cells.	bufalin	36-43	DNA methyltransferase 3 alpha	Homo sapiens	167-173
28364352-10	2017	Our results provide the evidence of bufalin for anti-leukemia, its mechanism may involve in increasing WT1 methylation status which is related to the up-regulation of DNMT3a and DNMT3b proteins in erythroid leukemic HEL cells.	bufalin	36-43	DNA methyltransferase 3 beta	Homo sapiens	178-184
29169175-0	2017	Bufalin Inhibits Cellular Proliferation and Cancer Stem Cell-Like Phenotypes via Upregulation of MiR-203 in Glioma.	bufalin	0-7	microRNA 203a	Homo sapiens	97-104
28419994-10	2017	Bufalin induced apoptosis in NSCLC cells by activating caspase cascades and downregulating Mcl-1 expression.	bufalin	0-7	MCL1 apoptosis regulator, BCL2 family member	Homo sapiens	91-96
28419994-11	2017	However, overexpression of Mcl-1 diminished bufalin-induced apoptosis.	bufalin	44-51	MCL1 apoptosis regulator, BCL2 family member	Homo sapiens	27-32
28419994-14	2017	Bufalin did not significantly affect NOXA protein levels, but downregulated the expression of p-GSK-3beta.	bufalin	0-7	glycogen synthase kinase 3 beta	Homo sapiens	96-105
28419994-15	2017	GSK-3 inhibitor and GSK-3beta siRNA resulted in increased levels of Mcl-1 and reversed the bufalin-induced Mcl-1 degradation.	bufalin	91-98	glycogen synthase kinase 3 beta	Homo sapiens	20-29
28419994-15	2017	GSK-3 inhibitor and GSK-3beta siRNA resulted in increased levels of Mcl-1 and reversed the bufalin-induced Mcl-1 degradation.	bufalin	91-98	MCL1 apoptosis regulator, BCL2 family member	Homo sapiens	68-73
28419994-15	2017	GSK-3 inhibitor and GSK-3beta siRNA resulted in increased levels of Mcl-1 and reversed the bufalin-induced Mcl-1 degradation.	bufalin	91-98	MCL1 apoptosis regulator, BCL2 family member	Homo sapiens	107-112
28419994-17	2017	Proteasomal degradation of Mcl-1 via GSK-3beta activation was involved in bufalin-induced apoptosis.	bufalin	74-81	MCL1 apoptosis regulator, BCL2 family member	Homo sapiens	27-32
28419994-17	2017	Proteasomal degradation of Mcl-1 via GSK-3beta activation was involved in bufalin-induced apoptosis.	bufalin	74-81	glycogen synthase kinase 3 beta	Homo sapiens	37-46
29169175-10	2017	Furthermore, overexpression of miR-203 led to similar outcomes as bufalin treatment with respect to the cell viability, colony formation, cell apoptosis and the phenotypes of glioma cells.	bufalin	66-73	microRNA 203a	Homo sapiens	31-38
29169175-11	2017	While anti-miR-203 attenuated the inhibitory effects of bufalin as promoting cell proliferation, colony formation and CSC phenotyes and inhibiting cell apoptosis.	bufalin	56-63	microRNA 203a	Homo sapiens	11-18
27821648-5	2017	Using MB-231 breast cancer cells, bufalin augments PKC induction of COX-2/IL-8 at both the protein and mRNA levels, and the production of prostaglandin E2 (PGE2) and IL-8.	bufalin	34-41	prostaglandin-endoperoxide synthase 2	Homo sapiens	68-73
27821648-5	2017	Using MB-231 breast cancer cells, bufalin augments PKC induction of COX-2/IL-8 at both the protein and mRNA levels, and the production of prostaglandin E2 (PGE2) and IL-8.	bufalin	34-41	C-X-C motif chemokine ligand 8	Homo sapiens	74-78
27686271-0	2016	Bufalin inhibits pancreatic cancer by inducing cell cycle arrest via the c-Myc/NF-kappaB pathway.	bufalin	0-7	MYC proto-oncogene, bHLH transcription factor	Homo sapiens	73-78
27821648-5	2017	Using MB-231 breast cancer cells, bufalin augments PKC induction of COX-2/IL-8 at both the protein and mRNA levels, and the production of prostaglandin E2 (PGE2) and IL-8.	bufalin	34-41	C-X-C motif chemokine ligand 8	Homo sapiens	166-170
28049205-0	2017	Bufalin Inhibits the Inflammatory Effects in Asthmatic Mice through the Suppression of Nuclear Factor-Kappa B Activity.	bufalin	0-7	nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105	Mus musculus	87-109
27686271-0	2016	Bufalin inhibits pancreatic cancer by inducing cell cycle arrest via the c-Myc/NF-kappaB pathway.	bufalin	0-7	nuclear factor kappa B subunit 1	Homo sapiens	79-88
27686271-8	2016	Bufalin treatment inhibited cyclin D1 and cyclin E1 expression and therefore increased expression of p27, a regulatory molecular that controls cell cycle transition from S to G2 phase.	bufalin	0-7	cyclin D1	Homo sapiens	28-37
27686271-8	2016	Bufalin treatment inhibited cyclin D1 and cyclin E1 expression and therefore increased expression of p27, a regulatory molecular that controls cell cycle transition from S to G2 phase.	bufalin	0-7	cyclin E1	Homo sapiens	42-51
27686271-8	2016	Bufalin treatment inhibited cyclin D1 and cyclin E1 expression and therefore increased expression of p27, a regulatory molecular that controls cell cycle transition from S to G2 phase.	bufalin	0-7	interferon alpha inducible protein 27	Homo sapiens	101-104
27686271-9	2016	Furthermore, luciferase reporter screening studies revealed that bufalin inhibited the expression and activity of the transcription factors c-Myc and NF-kappaB, which might cause cell cycle arrest at S phase and the inhibition of cell proliferation.	bufalin	65-72	MYC proto-oncogene, bHLH transcription factor	Homo sapiens	140-145
27686271-9	2016	Furthermore, luciferase reporter screening studies revealed that bufalin inhibited the expression and activity of the transcription factors c-Myc and NF-kappaB, which might cause cell cycle arrest at S phase and the inhibition of cell proliferation.	bufalin	65-72	nuclear factor kappa B subunit 1	Homo sapiens	150-159
27507851-7	2016	Among these 36 genes, apolipoprotein E (APOE) played a critical role, as knockdown APOE expression induced colocalization of Na+/K+-ATPase and caveolin and increased sensitivity of Hep3B cells to both proliferation-inhibiting and cytotoxic effects of BF or BF211.	bufalin	251-253	apolipoprotein E	Homo sapiens	22-38
27507851-7	2016	Among these 36 genes, apolipoprotein E (APOE) played a critical role, as knockdown APOE expression induced colocalization of Na+/K+-ATPase and caveolin and increased sensitivity of Hep3B cells to both proliferation-inhibiting and cytotoxic effects of BF or BF211.	bufalin	251-253	apolipoprotein E	Homo sapiens	40-44
27507851-7	2016	Among these 36 genes, apolipoprotein E (APOE) played a critical role, as knockdown APOE expression induced colocalization of Na+/K+-ATPase and caveolin and increased sensitivity of Hep3B cells to both proliferation-inhibiting and cytotoxic effects of BF or BF211.	bufalin	251-253	apolipoprotein E	Homo sapiens	83-87
27507851-8	2016	Also, the effects of BF on PI3K/AKT/GSK3beta and apoptosis signal cascades were enhanced in APOE knockdown cells.	bufalin	21-23	AKT serine/threonine kinase 1	Homo sapiens	32-35
27507851-8	2016	Also, the effects of BF on PI3K/AKT/GSK3beta and apoptosis signal cascades were enhanced in APOE knockdown cells.	bufalin	21-23	glycogen synthase kinase 3 beta	Homo sapiens	36-44
27507851-8	2016	Also, the effects of BF on PI3K/AKT/GSK3beta and apoptosis signal cascades were enhanced in APOE knockdown cells.	bufalin	21-23	apolipoprotein E	Homo sapiens	92-96
27425252-6	2016	Furthermore, we found that SRC-3 inhibitor bufalin increased cancer cell apoptosis induced by HDAC inhibitors.	bufalin	43-50	nuclear receptor coactivator 3	Homo sapiens	27-32
27571744-8	2016	Lentiviral-mediated miR-127-3p overexpression exerted tumor-suppressive effects in OVCAR-3 and Caov-3 cells by reducing in vitro proliferation and invasion, increasing bufalin sensitivity, and inhibiting in vivo tumor growth.	bufalin	168-175	microRNA 1273a	Homo sapiens	20-30
27634330-7	2016	Bufalin could downregulate the downstream target molecules of MMP-2, MMP-9, beta-catenin and VEGF in liver cancer cells by influencing the Gli1 and Gli3 expression of Hh signaling pathway, and upregulate the E-cadherin expression of liver cancer cells by influencing the Gli3 expression of Hh signaling pathway.	bufalin	0-7	GLI family zinc finger 1	Homo sapiens	139-143
27634330-7	2016	Bufalin could downregulate the downstream target molecules of MMP-2, MMP-9, beta-catenin and VEGF in liver cancer cells by influencing the Gli1 and Gli3 expression of Hh signaling pathway, and upregulate the E-cadherin expression of liver cancer cells by influencing the Gli3 expression of Hh signaling pathway.	bufalin	0-7	GLI family zinc finger 3	Homo sapiens	148-152
27634330-7	2016	Bufalin could downregulate the downstream target molecules of MMP-2, MMP-9, beta-catenin and VEGF in liver cancer cells by influencing the Gli1 and Gli3 expression of Hh signaling pathway, and upregulate the E-cadherin expression of liver cancer cells by influencing the Gli3 expression of Hh signaling pathway.	bufalin	0-7	cadherin 1	Homo sapiens	208-218
27634330-7	2016	Bufalin could downregulate the downstream target molecules of MMP-2, MMP-9, beta-catenin and VEGF in liver cancer cells by influencing the Gli1 and Gli3 expression of Hh signaling pathway, and upregulate the E-cadherin expression of liver cancer cells by influencing the Gli3 expression of Hh signaling pathway.	bufalin	0-7	GLI family zinc finger 3	Homo sapiens	271-275
27821648-6	2017	The MAPK and NF-kappaB pathways are involved in both the PKC-mediated and bufalin-promoted PKC regulation of COX-2/IL-8 production.	bufalin	74-81	prostaglandin-endoperoxide synthase 2	Homo sapiens	109-114
27821648-6	2017	The MAPK and NF-kappaB pathways are involved in both the PKC-mediated and bufalin-promoted PKC regulation of COX-2/IL-8 production.	bufalin	74-81	C-X-C motif chemokine ligand 8	Homo sapiens	115-119
27821648-10	2017	Bufalin significantly enhances breast cancer xenograft growth, which is accompanied by an elevation in COX-2/IL-8 expression.	bufalin	0-7	prostaglandin-endoperoxide synthase 2	Homo sapiens	103-108
27821648-10	2017	Bufalin significantly enhances breast cancer xenograft growth, which is accompanied by an elevation in COX-2/IL-8 expression.	bufalin	0-7	C-X-C motif chemokine ligand 8	Homo sapiens	109-113
27904697-3	2016	Treatment of human retinoblastoma (HXO-RB44) cells with bufalin induced apoptosis which was accompanied by a decrease in mitochondrial membrane potential, activation of caspase-9, caspase-8 and caspase-3, as well as changes in the expression of cytochrome C.	bufalin	56-63	caspase 9	Homo sapiens	169-178
27904697-3	2016	Treatment of human retinoblastoma (HXO-RB44) cells with bufalin induced apoptosis which was accompanied by a decrease in mitochondrial membrane potential, activation of caspase-9, caspase-8 and caspase-3, as well as changes in the expression of cytochrome C.	bufalin	56-63	caspase 8	Homo sapiens	180-189
27904697-3	2016	Treatment of human retinoblastoma (HXO-RB44) cells with bufalin induced apoptosis which was accompanied by a decrease in mitochondrial membrane potential, activation of caspase-9, caspase-8 and caspase-3, as well as changes in the expression of cytochrome C.	bufalin	56-63	caspase 3	Homo sapiens	194-203
27904697-3	2016	Treatment of human retinoblastoma (HXO-RB44) cells with bufalin induced apoptosis which was accompanied by a decrease in mitochondrial membrane potential, activation of caspase-9, caspase-8 and caspase-3, as well as changes in the expression of cytochrome C.	bufalin	56-63	cytochrome c, somatic	Homo sapiens	245-257
27904697-4	2016	Bufalin induced the cleavage of caspase-3 and apoptosis, and it was inhibited by both Z-LETD-FMK and Z-IETD-FMK treatment.	bufalin	0-7	caspase 3	Homo sapiens	32-41
27425252-6	2016	Furthermore, we found that SRC-3 inhibitor bufalin increased cancer cell apoptosis induced by HDAC inhibitors.	bufalin	43-50	histone deacetylase 9	Homo sapiens	94-98
27425252-7	2016	The combination of bufalin and SAHA was particular efficient in attenuating AKT activation and reducing Bcl-2 levels.	bufalin	19-26	AKT serine/threonine kinase 1	Homo sapiens	76-79
27425252-7	2016	The combination of bufalin and SAHA was particular efficient in attenuating AKT activation and reducing Bcl-2 levels.	bufalin	19-26	BCL2 apoptosis regulator	Homo sapiens	104-109
26662961-3	2016	In this research, we studied the effects of arenobufagin and bufalin on cellular survival and proliferation of HER2 overexpressing breast cancer cells and the mechanism under the results including the direct effect on HER2 downstream pathways.	bufalin	61-68	erb-b2 receptor tyrosine kinase 2	Homo sapiens	111-115
27634330-7	2016	Bufalin could downregulate the downstream target molecules of MMP-2, MMP-9, beta-catenin and VEGF in liver cancer cells by influencing the Gli1 and Gli3 expression of Hh signaling pathway, and upregulate the E-cadherin expression of liver cancer cells by influencing the Gli3 expression of Hh signaling pathway.	bufalin	0-7	matrix metallopeptidase 2	Homo sapiens	62-67
27634330-7	2016	Bufalin could downregulate the downstream target molecules of MMP-2, MMP-9, beta-catenin and VEGF in liver cancer cells by influencing the Gli1 and Gli3 expression of Hh signaling pathway, and upregulate the E-cadherin expression of liver cancer cells by influencing the Gli3 expression of Hh signaling pathway.	bufalin	0-7	matrix metallopeptidase 9	Homo sapiens	69-74
27634330-7	2016	Bufalin could downregulate the downstream target molecules of MMP-2, MMP-9, beta-catenin and VEGF in liver cancer cells by influencing the Gli1 and Gli3 expression of Hh signaling pathway, and upregulate the E-cadherin expression of liver cancer cells by influencing the Gli3 expression of Hh signaling pathway.	bufalin	0-7	catenin beta 1	Homo sapiens	76-88
27634330-7	2016	Bufalin could downregulate the downstream target molecules of MMP-2, MMP-9, beta-catenin and VEGF in liver cancer cells by influencing the Gli1 and Gli3 expression of Hh signaling pathway, and upregulate the E-cadherin expression of liver cancer cells by influencing the Gli3 expression of Hh signaling pathway.	bufalin	0-7	vascular endothelial growth factor A	Homo sapiens	93-97
27432228-8	2016	Additionally, the expression levels of CD24 and ESA were inhibited in the bufalin-treated mice.	bufalin	74-81	CD24a antigen	Mus musculus	39-43
27357249-0	2016	Bufalin reverses intrinsic and acquired drug resistance to cisplatin through the AKT signaling pathway in gastric cancer cells.	bufalin	0-7	AKT serine/threonine kinase 1	Homo sapiens	81-84
27357249-2	2016	Bufalin, a traditional Chinese medicine, shows significant anticancer activity by inhibiting the AKT pathway.	bufalin	0-7	AKT serine/threonine kinase 1	Homo sapiens	97-100
27357249-6	2016	It was demonstrated that bufalin synergized with cisplatin to inhibit proliferation and promote apoptosis of GC cells by diminishing the activation of cisplatin-induced AKT under normoxic and hypoxic conditions.	bufalin	25-32	AKT serine/threonine kinase 1	Homo sapiens	169-172
27357249-7	2016	Bufalin also inhibits cisplatin-activated molecules downstream of AKT that affect proliferation and apoptosis, including glycogen synthase kinase, mammalian target of rapamycin, ribosomal protein S6 Kinase and eukaryotic translation initiation factor-4E-binding protein-1.	bufalin	0-7	AKT serine/threonine kinase 1	Homo sapiens	66-69
27357249-7	2016	Bufalin also inhibits cisplatin-activated molecules downstream of AKT that affect proliferation and apoptosis, including glycogen synthase kinase, mammalian target of rapamycin, ribosomal protein S6 Kinase and eukaryotic translation initiation factor-4E-binding protein-1.	bufalin	0-7	mechanistic target of rapamycin kinase	Homo sapiens	147-176
27357249-7	2016	Bufalin also inhibits cisplatin-activated molecules downstream of AKT that affect proliferation and apoptosis, including glycogen synthase kinase, mammalian target of rapamycin, ribosomal protein S6 Kinase and eukaryotic translation initiation factor-4E-binding protein-1.	bufalin	0-7	eukaryotic translation initiation factor 4E binding protein 1	Homo sapiens	210-271
27459387-7	2016	The time-dependent effects of BF or BF211 on the activation of Src, which was mediated by the Na+/K+-ATPase signalosome, in A549 cells were also different.	bufalin	30-32	Rous sarcoma oncogene	Mus musculus	63-66
27238210-0	2016	Bufalin derivative BF211 inhibits proteasome activity in human lung cancer cells in vitro by inhibiting beta1 subunit expression and disrupting proteasome assembly.	bufalin	0-7	potassium calcium-activated channel subfamily M regulatory beta subunit 1	Homo sapiens	104-109
26662961-4	2016	Our results showed that arenobufagin and bufalin could significantly inhibit the proliferation and survival of HER2 overexpressing breast cancer cells, along with the declination of SRC-1, SRC-3, nuclear transcription factor E2F1, phosphorylated AKT, and ERK.	bufalin	41-48	erb-b2 receptor tyrosine kinase 2	Homo sapiens	111-115
26662961-4	2016	Our results showed that arenobufagin and bufalin could significantly inhibit the proliferation and survival of HER2 overexpressing breast cancer cells, along with the declination of SRC-1, SRC-3, nuclear transcription factor E2F1, phosphorylated AKT, and ERK.	bufalin	41-48	SRC proto-oncogene, non-receptor tyrosine kinase	Homo sapiens	182-187
26662961-4	2016	Our results showed that arenobufagin and bufalin could significantly inhibit the proliferation and survival of HER2 overexpressing breast cancer cells, along with the declination of SRC-1, SRC-3, nuclear transcription factor E2F1, phosphorylated AKT, and ERK.	bufalin	41-48	nuclear receptor coactivator 3	Homo sapiens	189-194
26662961-4	2016	Our results showed that arenobufagin and bufalin could significantly inhibit the proliferation and survival of HER2 overexpressing breast cancer cells, along with the declination of SRC-1, SRC-3, nuclear transcription factor E2F1, phosphorylated AKT, and ERK.	bufalin	41-48	E2F transcription factor 1	Homo sapiens	225-229
26662961-4	2016	Our results showed that arenobufagin and bufalin could significantly inhibit the proliferation and survival of HER2 overexpressing breast cancer cells, along with the declination of SRC-1, SRC-3, nuclear transcription factor E2F1, phosphorylated AKT, and ERK.	bufalin	41-48	AKT serine/threonine kinase 1	Homo sapiens	246-249
26662961-4	2016	Our results showed that arenobufagin and bufalin could significantly inhibit the proliferation and survival of HER2 overexpressing breast cancer cells, along with the declination of SRC-1, SRC-3, nuclear transcription factor E2F1, phosphorylated AKT, and ERK.	bufalin	41-48	mitogen-activated protein kinase 1	Homo sapiens	255-258
26662961-6	2016	All above suggest that arenobufagin and bufalin may be potential therapy adjuvants for HER2 overexpressing breast cancer therapy.	bufalin	40-47	erb-b2 receptor tyrosine kinase 2	Homo sapiens	87-91
27188603-0	2016	[Effect of bufalin on proliferation and apoptosis through ERK/RSK2 pathway in human esophageal carcinoma cell line xenografts in nude mice].	bufalin	11-18	mitogen-activated protein kinase 1	Homo sapiens	58-61
26782953-0	2016	Bufalin enhances anti-angiogenic effect of sorafenib via AKT/VEGF signaling.	bufalin	0-7	AKT serine/threonine kinase 1	Homo sapiens	57-60
27188603-0	2016	[Effect of bufalin on proliferation and apoptosis through ERK/RSK2 pathway in human esophageal carcinoma cell line xenografts in nude mice].	bufalin	11-18	ribosomal protein S6 kinase A3	Homo sapiens	62-66
27188603-1	2016	OBJECTIVE: To investigate the effect of bufalin on proliferation and apoptosis through ERK/RSK2 pathway in esophageal squamous cell carcinoma xenografts in nude mice.	bufalin	40-47	mitogen-activated protein kinase 1	Mus musculus	87-90
27188603-1	2016	OBJECTIVE: To investigate the effect of bufalin on proliferation and apoptosis through ERK/RSK2 pathway in esophageal squamous cell carcinoma xenografts in nude mice.	bufalin	40-47	ribosomal protein S6 kinase polypeptide 3	Mus musculus	91-95
27188603-21	2016	Bufalin may suppress the growth of xenogragts in nude mice by down-regulating the level of ERK and RSK2 phosphorylation, inhibit the proliferation of xenogragts via inactivating GSK3beta and promote apoptosis through down-regulation of p-Bad.	bufalin	0-7	mitogen-activated protein kinase 1	Mus musculus	91-94
27188603-21	2016	Bufalin may suppress the growth of xenogragts in nude mice by down-regulating the level of ERK and RSK2 phosphorylation, inhibit the proliferation of xenogragts via inactivating GSK3beta and promote apoptosis through down-regulation of p-Bad.	bufalin	0-7	ribosomal protein S6 kinase polypeptide 3	Mus musculus	99-103
27188603-21	2016	Bufalin may suppress the growth of xenogragts in nude mice by down-regulating the level of ERK and RSK2 phosphorylation, inhibit the proliferation of xenogragts via inactivating GSK3beta and promote apoptosis through down-regulation of p-Bad.	bufalin	0-7	glycogen synthase kinase 3 beta	Mus musculus	178-186
26958938-0	2016	Bufalin suppresses hepatocellular carcinoma invasion and metastasis by targeting HIF-1alpha via the PI3K/AKT/mTOR pathway.	bufalin	0-7	hypoxia inducible factor 1 subunit alpha	Homo sapiens	81-91
26958938-0	2016	Bufalin suppresses hepatocellular carcinoma invasion and metastasis by targeting HIF-1alpha via the PI3K/AKT/mTOR pathway.	bufalin	0-7	AKT serine/threonine kinase 1	Homo sapiens	105-108
26958938-0	2016	Bufalin suppresses hepatocellular carcinoma invasion and metastasis by targeting HIF-1alpha via the PI3K/AKT/mTOR pathway.	bufalin	0-7	mechanistic target of rapamycin kinase	Homo sapiens	109-113
26958938-6	2016	Furthermore, the epithelial-to-mesenchymal transition (EMT) was inhibited in bufalin-treated tumors, as reflected the upregulation of E-cadherin, and downregulation of N-cadherin, vimentin, Snail.	bufalin	77-84	cadherin 1	Homo sapiens	134-144
26958938-6	2016	Furthermore, the epithelial-to-mesenchymal transition (EMT) was inhibited in bufalin-treated tumors, as reflected the upregulation of E-cadherin, and downregulation of N-cadherin, vimentin, Snail.	bufalin	77-84	cadherin 2	Homo sapiens	168-178
26958938-6	2016	Furthermore, the epithelial-to-mesenchymal transition (EMT) was inhibited in bufalin-treated tumors, as reflected the upregulation of E-cadherin, and downregulation of N-cadherin, vimentin, Snail.	bufalin	77-84	vimentin	Homo sapiens	180-188
26958938-6	2016	Furthermore, the epithelial-to-mesenchymal transition (EMT) was inhibited in bufalin-treated tumors, as reflected the upregulation of E-cadherin, and downregulation of N-cadherin, vimentin, Snail.	bufalin	77-84	snail family transcriptional repressor 1	Homo sapiens	190-195
26958938-9	2016	Mechanistically, our study demonstrated that hypoxia-inducible factor-1alpha (HIF-1alpha) played an important role in the antimetastatic effect of bufalin in hepatocellular carcinoma.	bufalin	147-154	hypoxia inducible factor 1 subunit alpha	Homo sapiens	45-76
26958938-9	2016	Mechanistically, our study demonstrated that hypoxia-inducible factor-1alpha (HIF-1alpha) played an important role in the antimetastatic effect of bufalin in hepatocellular carcinoma.	bufalin	147-154	hypoxia inducible factor 1 subunit alpha	Homo sapiens	78-88
26958938-11	2016	Taken together, our results suggest that bufalin suppresses hepatic tumor invasion and metastasis and that this process may be related to the PI3K/AKT/mTOR/ HIF-1alpha axis.	bufalin	41-48	AKT serine/threonine kinase 1	Homo sapiens	147-150
26958938-11	2016	Taken together, our results suggest that bufalin suppresses hepatic tumor invasion and metastasis and that this process may be related to the PI3K/AKT/mTOR/ HIF-1alpha axis.	bufalin	41-48	mechanistic target of rapamycin kinase	Homo sapiens	151-155
27186298-11	2016	In EOC cell lines, functional assays demonstrated that miR-183 downregulation inhibited cancer proliferation, enhanced bufalin sensitivity and reduced tumorigenicity in vivo.	bufalin	119-126	microRNA 183	Homo sapiens	55-62
26782953-0	2016	Bufalin enhances anti-angiogenic effect of sorafenib via AKT/VEGF signaling.	bufalin	0-7	vascular endothelial growth factor A	Homo sapiens	61-65
26782953-15	2016	Taken together, our findings demonstrated for the first time that bufalin can enhance anti-angiogenic effect of sorafenib via modulating the AKT/VEGF signaling pathway.	bufalin	66-73	AKT serine/threonine kinase 1	Homo sapiens	141-144
26782953-15	2016	Taken together, our findings demonstrated for the first time that bufalin can enhance anti-angiogenic effect of sorafenib via modulating the AKT/VEGF signaling pathway.	bufalin	66-73	vascular endothelial growth factor A	Homo sapiens	145-149
26648158-4	2016	Bufalin-induced apoptosis was accompanied with a significant reduction of the mitochondrial membrane potential, release of mitochondrial cytochrome c into the cytosol, activation of caspase-3, caspase-9 and poly(adenosine diphosphate ribose) polymerase, as well as downregulation of B-cell lymphoma 2 (Bcl-2)/Bcl-2-associated X protein.	bufalin	0-7	cytochrome c, somatic	Homo sapiens	137-149
26398931-0	2016	miR-155-5p antagonizes the apoptotic effect of bufalin in triple-negative breast cancer cells.	bufalin	47-54	microRNA 155	Homo sapiens	0-7
26398931-3	2016	In this study, we showed that bufalin induced apoptosis in MDA-MB-231 and MCF-7/ADR TNBC cell lines, accompanied by increased miR-155-5p expression.	bufalin	30-37	microRNA 155	Homo sapiens	126-133
26398931-4	2016	Overexpression of miR-155-5p promoted proliferation and reduced bufalin-induced apoptosis of TNBC cells.	bufalin	64-71	microRNA 155	Homo sapiens	18-25
26398931-5	2016	In contrast, downregulation of miR-155-5p increased sensitivity to bufalin and upregulated the expression of FOXO3A.	bufalin	67-74	microRNA 155	Homo sapiens	31-38
26398931-6	2016	Bufalin also downregulated DNA methyltransferases 1 and 3a (DNMT1 and DNMT3a), and concurrent inhibition of DNMT1 and DNMT3a significantly increased miR-155-5p expression.	bufalin	0-7	DNA methyltransferase 1	Homo sapiens	27-58
26398931-6	2016	Bufalin also downregulated DNA methyltransferases 1 and 3a (DNMT1 and DNMT3a), and concurrent inhibition of DNMT1 and DNMT3a significantly increased miR-155-5p expression.	bufalin	0-7	DNA methyltransferase 1	Homo sapiens	60-65
26398931-6	2016	Bufalin also downregulated DNA methyltransferases 1 and 3a (DNMT1 and DNMT3a), and concurrent inhibition of DNMT1 and DNMT3a significantly increased miR-155-5p expression.	bufalin	0-7	DNA methyltransferase 3 alpha	Homo sapiens	70-76
26398931-6	2016	Bufalin also downregulated DNA methyltransferases 1 and 3a (DNMT1 and DNMT3a), and concurrent inhibition of DNMT1 and DNMT3a significantly increased miR-155-5p expression.	bufalin	0-7	microRNA 155	Homo sapiens	149-156
26398931-7	2016	These results indicate that miR-155-5p antagonizes bufalin sensitivity in TNBC cells, and that downregulation of DNMT1 and DNMT3a may be responsible for the bufalin-induced upregulation of miR-155-5p.	bufalin	157-164	microRNA 155	Homo sapiens	28-35
26398931-7	2016	These results indicate that miR-155-5p antagonizes bufalin sensitivity in TNBC cells, and that downregulation of DNMT1 and DNMT3a may be responsible for the bufalin-induced upregulation of miR-155-5p.	bufalin	157-164	DNA methyltransferase 1	Homo sapiens	113-118
26398931-7	2016	These results indicate that miR-155-5p antagonizes bufalin sensitivity in TNBC cells, and that downregulation of DNMT1 and DNMT3a may be responsible for the bufalin-induced upregulation of miR-155-5p.	bufalin	157-164	DNA methyltransferase 3 alpha	Homo sapiens	123-129
26398931-7	2016	These results indicate that miR-155-5p antagonizes bufalin sensitivity in TNBC cells, and that downregulation of DNMT1 and DNMT3a may be responsible for the bufalin-induced upregulation of miR-155-5p.	bufalin	157-164	microRNA 155	Homo sapiens	189-196
27997899-0	2016	Bufalin Inhibits hTERT Expression and Colorectal Cancer Cell Growth by Targeting CPSF4.	bufalin	0-7	telomerase reverse transcriptase	Homo sapiens	17-22
27997899-0	2016	Bufalin Inhibits hTERT Expression and Colorectal Cancer Cell Growth by Targeting CPSF4.	bufalin	0-7	cleavage and polyadenylation specific factor 4	Homo sapiens	81-86
28074104-3	2016	By microRNA (miR) array analysis and quantitative reverse transcription polymerase chain reaction, we found that miR-221 was downregulated after treatment with bufalin.	bufalin	160-167	microRNA 221	Homo sapiens	113-120
28074104-5	2016	Furthermore, upregulating miR-221 by its MIMIC and suppressing BBC3 by small interfering RNA (siRNA) reversed the effects of bufalin on osteosarcoma cells.	bufalin	125-132	microRNA 221	Homo sapiens	26-33
28074104-5	2016	Furthermore, upregulating miR-221 by its MIMIC and suppressing BBC3 by small interfering RNA (siRNA) reversed the effects of bufalin on osteosarcoma cells.	bufalin	125-132	BCL2 binding component 3	Homo sapiens	63-67
28074104-6	2016	Collectively, our data indicate that bufalin inhibits cell proliferation and induces mitochondria-dependent apoptosis in osteosarcoma cells through downregulating miR-221 and triggering BBC3 expression.	bufalin	37-44	microRNA 221	Homo sapiens	163-170
28074104-6	2016	Collectively, our data indicate that bufalin inhibits cell proliferation and induces mitochondria-dependent apoptosis in osteosarcoma cells through downregulating miR-221 and triggering BBC3 expression.	bufalin	37-44	BCL2 binding component 3	Homo sapiens	186-190
26648168-0	2016	The effect and mechanism of bufalin on regulating hepatocellular carcinoma cell invasion and metastasis via Wnt/beta-catenin signaling pathway.	bufalin	28-35	catenin beta 1	Homo sapiens	112-124
26648168-5	2016	Our results indicate that: i) bufalin can regulate the expression of associated factors in Wnt/beta-catenin signaling pathway of BEL-7402 cells through suppressing the phosphorylation of GSK-3beta Ser9 site; ii) bufalin can strengthen intercellular E-cadherin/beta-catenin complex to control epithelial-mesenchymal transition; and iii) bufalin can reverse the malignant phenotype and promote the differentiation and maturation by regulating the AFP and ALB expression in BEL-7402 cells.	bufalin	30-37	catenin beta 1	Homo sapiens	95-107
26648168-5	2016	Our results indicate that: i) bufalin can regulate the expression of associated factors in Wnt/beta-catenin signaling pathway of BEL-7402 cells through suppressing the phosphorylation of GSK-3beta Ser9 site; ii) bufalin can strengthen intercellular E-cadherin/beta-catenin complex to control epithelial-mesenchymal transition; and iii) bufalin can reverse the malignant phenotype and promote the differentiation and maturation by regulating the AFP and ALB expression in BEL-7402 cells.	bufalin	30-37	glycogen synthase kinase 3 beta	Homo sapiens	187-196
26648168-5	2016	Our results indicate that: i) bufalin can regulate the expression of associated factors in Wnt/beta-catenin signaling pathway of BEL-7402 cells through suppressing the phosphorylation of GSK-3beta Ser9 site; ii) bufalin can strengthen intercellular E-cadherin/beta-catenin complex to control epithelial-mesenchymal transition; and iii) bufalin can reverse the malignant phenotype and promote the differentiation and maturation by regulating the AFP and ALB expression in BEL-7402 cells.	bufalin	30-37	cadherin 1	Homo sapiens	249-259
26648168-5	2016	Our results indicate that: i) bufalin can regulate the expression of associated factors in Wnt/beta-catenin signaling pathway of BEL-7402 cells through suppressing the phosphorylation of GSK-3beta Ser9 site; ii) bufalin can strengthen intercellular E-cadherin/beta-catenin complex to control epithelial-mesenchymal transition; and iii) bufalin can reverse the malignant phenotype and promote the differentiation and maturation by regulating the AFP and ALB expression in BEL-7402 cells.	bufalin	30-37	catenin beta 1	Homo sapiens	260-272
26648168-5	2016	Our results indicate that: i) bufalin can regulate the expression of associated factors in Wnt/beta-catenin signaling pathway of BEL-7402 cells through suppressing the phosphorylation of GSK-3beta Ser9 site; ii) bufalin can strengthen intercellular E-cadherin/beta-catenin complex to control epithelial-mesenchymal transition; and iii) bufalin can reverse the malignant phenotype and promote the differentiation and maturation by regulating the AFP and ALB expression in BEL-7402 cells.	bufalin	30-37	alpha fetoprotein	Homo sapiens	445-448
26648168-5	2016	Our results indicate that: i) bufalin can regulate the expression of associated factors in Wnt/beta-catenin signaling pathway of BEL-7402 cells through suppressing the phosphorylation of GSK-3beta Ser9 site; ii) bufalin can strengthen intercellular E-cadherin/beta-catenin complex to control epithelial-mesenchymal transition; and iii) bufalin can reverse the malignant phenotype and promote the differentiation and maturation by regulating the AFP and ALB expression in BEL-7402 cells.	bufalin	30-37	albumin	Homo sapiens	453-456
26648168-5	2016	Our results indicate that: i) bufalin can regulate the expression of associated factors in Wnt/beta-catenin signaling pathway of BEL-7402 cells through suppressing the phosphorylation of GSK-3beta Ser9 site; ii) bufalin can strengthen intercellular E-cadherin/beta-catenin complex to control epithelial-mesenchymal transition; and iii) bufalin can reverse the malignant phenotype and promote the differentiation and maturation by regulating the AFP and ALB expression in BEL-7402 cells.	bufalin	212-219	catenin beta 1	Homo sapiens	95-107
26648168-5	2016	Our results indicate that: i) bufalin can regulate the expression of associated factors in Wnt/beta-catenin signaling pathway of BEL-7402 cells through suppressing the phosphorylation of GSK-3beta Ser9 site; ii) bufalin can strengthen intercellular E-cadherin/beta-catenin complex to control epithelial-mesenchymal transition; and iii) bufalin can reverse the malignant phenotype and promote the differentiation and maturation by regulating the AFP and ALB expression in BEL-7402 cells.	bufalin	212-219	catenin beta 1	Homo sapiens	95-107
26787099-8	2016	Western blotting assay results confirmed BF-induced change in levels of fibronectin and other related proteins.	bufalin	41-43	fibronectin 1	Homo sapiens	72-83
26787099-9	2016	Overexpression of fibronectin by plasmid transfection ameliorated antimigration effects of BF.	bufalin	91-93	fibronectin 1	Homo sapiens	18-29
26758421-0	2016	Bufalin enhances antitumor effect of paclitaxel on cervical tumorigenesis via inhibiting the integrin alpha2/beta5/FAK signaling pathway.	bufalin	0-7	integrin subunit alpha 2	Homo sapiens	93-108
26758421-0	2016	Bufalin enhances antitumor effect of paclitaxel on cervical tumorigenesis via inhibiting the integrin alpha2/beta5/FAK signaling pathway.	bufalin	0-7	tubulin beta 4A class IVa	Homo sapiens	109-114
26758421-0	2016	Bufalin enhances antitumor effect of paclitaxel on cervical tumorigenesis via inhibiting the integrin alpha2/beta5/FAK signaling pathway.	bufalin	0-7	protein tyrosine kinase 2	Homo sapiens	115-118
26758421-2	2016	Here, we show that Bufalin can inhibit cervical cancer cell proliferation, block cell cycle in G2/M phase, induce cellular apoptosis and reduce cell metastasis through stimulation of p21(waf/cip1), p27(cip/kip), Bax and E-cadherin, and suppression of cyclin A, cyclin B1, CDK2, Bcl-2, Bcl-xl, MMP9 and SNAIL1.	bufalin	19-26	H3 histone pseudogene 16	Homo sapiens	183-186
26758421-2	2016	Here, we show that Bufalin can inhibit cervical cancer cell proliferation, block cell cycle in G2/M phase, induce cellular apoptosis and reduce cell metastasis through stimulation of p21(waf/cip1), p27(cip/kip), Bax and E-cadherin, and suppression of cyclin A, cyclin B1, CDK2, Bcl-2, Bcl-xl, MMP9 and SNAIL1.	bufalin	19-26	cyclin dependent kinase inhibitor 1A	Homo sapiens	191-195
26758421-2	2016	Here, we show that Bufalin can inhibit cervical cancer cell proliferation, block cell cycle in G2/M phase, induce cellular apoptosis and reduce cell metastasis through stimulation of p21(waf/cip1), p27(cip/kip), Bax and E-cadherin, and suppression of cyclin A, cyclin B1, CDK2, Bcl-2, Bcl-xl, MMP9 and SNAIL1.	bufalin	19-26	interferon alpha inducible protein 27	Homo sapiens	198-201
26758421-2	2016	Here, we show that Bufalin can inhibit cervical cancer cell proliferation, block cell cycle in G2/M phase, induce cellular apoptosis and reduce cell metastasis through stimulation of p21(waf/cip1), p27(cip/kip), Bax and E-cadherin, and suppression of cyclin A, cyclin B1, CDK2, Bcl-2, Bcl-xl, MMP9 and SNAIL1.	bufalin	19-26	BCL2 associated X, apoptosis regulator	Homo sapiens	212-215
26758421-2	2016	Here, we show that Bufalin can inhibit cervical cancer cell proliferation, block cell cycle in G2/M phase, induce cellular apoptosis and reduce cell metastasis through stimulation of p21(waf/cip1), p27(cip/kip), Bax and E-cadherin, and suppression of cyclin A, cyclin B1, CDK2, Bcl-2, Bcl-xl, MMP9 and SNAIL1.	bufalin	19-26	cadherin 1	Homo sapiens	220-230
26758421-2	2016	Here, we show that Bufalin can inhibit cervical cancer cell proliferation, block cell cycle in G2/M phase, induce cellular apoptosis and reduce cell metastasis through stimulation of p21(waf/cip1), p27(cip/kip), Bax and E-cadherin, and suppression of cyclin A, cyclin B1, CDK2, Bcl-2, Bcl-xl, MMP9 and SNAIL1.	bufalin	19-26	cyclin A2	Homo sapiens	251-259
26758421-2	2016	Here, we show that Bufalin can inhibit cervical cancer cell proliferation, block cell cycle in G2/M phase, induce cellular apoptosis and reduce cell metastasis through stimulation of p21(waf/cip1), p27(cip/kip), Bax and E-cadherin, and suppression of cyclin A, cyclin B1, CDK2, Bcl-2, Bcl-xl, MMP9 and SNAIL1.	bufalin	19-26	cyclin B1	Homo sapiens	261-270
26758421-2	2016	Here, we show that Bufalin can inhibit cervical cancer cell proliferation, block cell cycle in G2/M phase, induce cellular apoptosis and reduce cell metastasis through stimulation of p21(waf/cip1), p27(cip/kip), Bax and E-cadherin, and suppression of cyclin A, cyclin B1, CDK2, Bcl-2, Bcl-xl, MMP9 and SNAIL1.	bufalin	19-26	cyclin dependent kinase 2	Homo sapiens	272-276
26758421-2	2016	Here, we show that Bufalin can inhibit cervical cancer cell proliferation, block cell cycle in G2/M phase, induce cellular apoptosis and reduce cell metastasis through stimulation of p21(waf/cip1), p27(cip/kip), Bax and E-cadherin, and suppression of cyclin A, cyclin B1, CDK2, Bcl-2, Bcl-xl, MMP9 and SNAIL1.	bufalin	19-26	BCL2 apoptosis regulator	Homo sapiens	278-283
26758421-2	2016	Here, we show that Bufalin can inhibit cervical cancer cell proliferation, block cell cycle in G2/M phase, induce cellular apoptosis and reduce cell metastasis through stimulation of p21(waf/cip1), p27(cip/kip), Bax and E-cadherin, and suppression of cyclin A, cyclin B1, CDK2, Bcl-2, Bcl-xl, MMP9 and SNAIL1.	bufalin	19-26	BCL2 like 1	Homo sapiens	285-291
26758421-2	2016	Here, we show that Bufalin can inhibit cervical cancer cell proliferation, block cell cycle in G2/M phase, induce cellular apoptosis and reduce cell metastasis through stimulation of p21(waf/cip1), p27(cip/kip), Bax and E-cadherin, and suppression of cyclin A, cyclin B1, CDK2, Bcl-2, Bcl-xl, MMP9 and SNAIL1.	bufalin	19-26	matrix metallopeptidase 9	Homo sapiens	293-297
26758421-2	2016	Here, we show that Bufalin can inhibit cervical cancer cell proliferation, block cell cycle in G2/M phase, induce cellular apoptosis and reduce cell metastasis through stimulation of p21(waf/cip1), p27(cip/kip), Bax and E-cadherin, and suppression of cyclin A, cyclin B1, CDK2, Bcl-2, Bcl-xl, MMP9 and SNAIL1.	bufalin	19-26	snail family transcriptional repressor 1	Homo sapiens	302-308
26758421-4	2016	Mechanistic study reveals that Bufalin suppresses the integrin alpha2/FAK/AKT1/ GSK3beta signaling.	bufalin	31-38	integrin subunit alpha 2	Homo sapiens	54-69
26758421-4	2016	Mechanistic study reveals that Bufalin suppresses the integrin alpha2/FAK/AKT1/ GSK3beta signaling.	bufalin	31-38	protein tyrosine kinase 2	Homo sapiens	70-73
26758421-4	2016	Mechanistic study reveals that Bufalin suppresses the integrin alpha2/FAK/AKT1/ GSK3beta signaling.	bufalin	31-38	AKT serine/threonine kinase 1	Homo sapiens	74-78
26758421-4	2016	Mechanistic study reveals that Bufalin suppresses the integrin alpha2/FAK/AKT1/ GSK3beta signaling.	bufalin	31-38	glycogen synthase kinase 3 beta	Homo sapiens	80-88
26648158-4	2016	Bufalin-induced apoptosis was accompanied with a significant reduction of the mitochondrial membrane potential, release of mitochondrial cytochrome c into the cytosol, activation of caspase-3, caspase-9 and poly(adenosine diphosphate ribose) polymerase, as well as downregulation of B-cell lymphoma 2 (Bcl-2)/Bcl-2-associated X protein.	bufalin	0-7	caspase 3	Homo sapiens	182-191
26648158-4	2016	Bufalin-induced apoptosis was accompanied with a significant reduction of the mitochondrial membrane potential, release of mitochondrial cytochrome c into the cytosol, activation of caspase-3, caspase-9 and poly(adenosine diphosphate ribose) polymerase, as well as downregulation of B-cell lymphoma 2 (Bcl-2)/Bcl-2-associated X protein.	bufalin	0-7	caspase 9	Homo sapiens	193-202
26648158-4	2016	Bufalin-induced apoptosis was accompanied with a significant reduction of the mitochondrial membrane potential, release of mitochondrial cytochrome c into the cytosol, activation of caspase-3, caspase-9 and poly(adenosine diphosphate ribose) polymerase, as well as downregulation of B-cell lymphoma 2 (Bcl-2)/Bcl-2-associated X protein.	bufalin	0-7	BCL2 apoptosis regulator	Homo sapiens	283-300
26648158-4	2016	Bufalin-induced apoptosis was accompanied with a significant reduction of the mitochondrial membrane potential, release of mitochondrial cytochrome c into the cytosol, activation of caspase-3, caspase-9 and poly(adenosine diphosphate ribose) polymerase, as well as downregulation of B-cell lymphoma 2 (Bcl-2)/Bcl-2-associated X protein.	bufalin	0-7	BCL2 apoptosis regulator	Homo sapiens	302-307
26648158-4	2016	Bufalin-induced apoptosis was accompanied with a significant reduction of the mitochondrial membrane potential, release of mitochondrial cytochrome c into the cytosol, activation of caspase-3, caspase-9 and poly(adenosine diphosphate ribose) polymerase, as well as downregulation of B-cell lymphoma 2 (Bcl-2)/Bcl-2-associated X protein.	bufalin	0-7	BCL2 apoptosis regulator	Homo sapiens	309-314
26133118-8	2015	Further analysis showed that the TGF-beta receptor I (TbetaRI) and TGF-beta receptor II (TbetaRII) were downregulated in the presence of bufalin.	bufalin	137-144	transforming growth factor beta receptor 2	Homo sapiens	67-87
26431029-5	2015	Furthermore, we demonstrated that bufalin, a SRC-3 small molecule inhibitor, when introduced even at nM concentrations, can significantly reduce TNBC cell viability and motility.	bufalin	34-41	nuclear receptor coactivator 3	Homo sapiens	45-50
26381511-0	2015	Bufalin Reverses Resistance to Sorafenib by Inhibiting Akt Activation in Hepatocellular Carcinoma: The Role of Endoplasmic Reticulum Stress.	bufalin	0-7	AKT serine/threonine kinase 1	Homo sapiens	55-58
26381511-3	2015	Bufalin is the major active ingredient of the traditional Chinese medicine Chan su, which inhibits Akt activation; therefore, Chan su is currently used in the clinic to treat cancer.	bufalin	0-7	AKT serine/threonine kinase 1	Homo sapiens	99-102
26381511-8	2015	Silencing IRE1 with siRNA blocked the bufalin-induced Akt inactivation, but silencing eukaryotic initiation factor 2 (eIF2) or C/EBP-homologous protein (CHOP) did not have the same effect.	bufalin	38-45	endoplasmic reticulum to nucleus signaling 1	Homo sapiens	10-14
26381511-8	2015	Silencing IRE1 with siRNA blocked the bufalin-induced Akt inactivation, but silencing eukaryotic initiation factor 2 (eIF2) or C/EBP-homologous protein (CHOP) did not have the same effect.	bufalin	38-45	AKT serine/threonine kinase 1	Homo sapiens	54-57
26381511-11	2015	Thus, Bufalin reversed acquired resistance to sorafenib by downregulating phosphorylated Akt in an ER-stress-dependent manner via the IRE1 pathway.	bufalin	6-13	AKT serine/threonine kinase 1	Homo sapiens	89-92
26381511-11	2015	Thus, Bufalin reversed acquired resistance to sorafenib by downregulating phosphorylated Akt in an ER-stress-dependent manner via the IRE1 pathway.	bufalin	6-13	endoplasmic reticulum to nucleus signaling 1	Homo sapiens	134-138
26133118-0	2015	Bufalin inhibits TGF-beta-induced epithelial-to-mesenchymal transition and migration in human lung cancer A549 cells by downregulating TGF-beta receptors.	bufalin	0-7	transforming growth factor beta 1	Homo sapiens	17-25
26133118-8	2015	Further analysis showed that the TGF-beta receptor I (TbetaRI) and TGF-beta receptor II (TbetaRII) were downregulated in the presence of bufalin.	bufalin	137-144	transforming growth factor beta receptor 2	Homo sapiens	89-97
25936899-0	2015	Secreted protein acidic and rich in cysteine antagonizes bufalin-induced apoptosis in gastric cancer cells.	bufalin	57-64	secreted protein acidic and cysteine rich	Homo sapiens	0-44
26133118-0	2015	Bufalin inhibits TGF-beta-induced epithelial-to-mesenchymal transition and migration in human lung cancer A549 cells by downregulating TGF-beta receptors.	bufalin	0-7	transforming growth factor beta 1	Homo sapiens	135-143
26133118-4	2015	In the present study, the effect of bufalin on TGF-beta-induced EMT and migration was investigated in human lung cancer A549 cells.	bufalin	36-43	transforming growth factor beta 1	Homo sapiens	47-55
26133118-5	2015	TGF-beta induced EMT in A549 cells and increased their migratory ability, which were markedly suppressed by bufalin.	bufalin	108-115	transforming growth factor beta 1	Homo sapiens	0-8
26133118-6	2015	Additionally, TGF-beta-induced upregulation of Twist2 and zinc finger E-box binding homeobox 2 (ZEB2), as well as the phosphorylation of Smad2 and Smad3 were also inhibited by bufalin.	bufalin	176-183	transforming growth factor beta 1	Homo sapiens	14-22
26133118-6	2015	Additionally, TGF-beta-induced upregulation of Twist2 and zinc finger E-box binding homeobox 2 (ZEB2), as well as the phosphorylation of Smad2 and Smad3 were also inhibited by bufalin.	bufalin	176-183	twist family bHLH transcription factor 2	Homo sapiens	47-53
26133118-6	2015	Additionally, TGF-beta-induced upregulation of Twist2 and zinc finger E-box binding homeobox 2 (ZEB2), as well as the phosphorylation of Smad2 and Smad3 were also inhibited by bufalin.	bufalin	176-183	zinc finger E-box binding homeobox 2	Homo sapiens	58-94
26133118-6	2015	Additionally, TGF-beta-induced upregulation of Twist2 and zinc finger E-box binding homeobox 2 (ZEB2), as well as the phosphorylation of Smad2 and Smad3 were also inhibited by bufalin.	bufalin	176-183	zinc finger E-box binding homeobox 2	Homo sapiens	96-100
26133118-6	2015	Additionally, TGF-beta-induced upregulation of Twist2 and zinc finger E-box binding homeobox 2 (ZEB2), as well as the phosphorylation of Smad2 and Smad3 were also inhibited by bufalin.	bufalin	176-183	SMAD family member 2	Homo sapiens	137-142
26133118-6	2015	Additionally, TGF-beta-induced upregulation of Twist2 and zinc finger E-box binding homeobox 2 (ZEB2), as well as the phosphorylation of Smad2 and Smad3 were also inhibited by bufalin.	bufalin	176-183	SMAD family member 3	Homo sapiens	147-152
26133118-8	2015	Further analysis showed that the TGF-beta receptor I (TbetaRI) and TGF-beta receptor II (TbetaRII) were downregulated in the presence of bufalin.	bufalin	137-144	transforming growth factor beta receptor 1	Homo sapiens	33-52
26133118-8	2015	Further analysis showed that the TGF-beta receptor I (TbetaRI) and TGF-beta receptor II (TbetaRII) were downregulated in the presence of bufalin.	bufalin	137-144	transforming growth factor beta receptor 1	Homo sapiens	54-61
25936899-4	2015	This study aimed to evaluate the role of SPARC in bufalin-induced apoptosis in SGC7901 and MGC803 gastric cancer cells.	bufalin	50-57	secreted protein acidic and cysteine rich	Homo sapiens	41-46
25936899-7	2015	Furthermore, it was shown that SPARC negatively regulated bufalin-induced intrinsic apoptosis by protecting mitochondrial integrity, decreasing the release of cytoplasmic cytochrome c and increasing the ratio of Bcl-2/Bax.	bufalin	58-65	secreted protein acidic and cysteine rich	Homo sapiens	31-36
25936899-7	2015	Furthermore, it was shown that SPARC negatively regulated bufalin-induced intrinsic apoptosis by protecting mitochondrial integrity, decreasing the release of cytoplasmic cytochrome c and increasing the ratio of Bcl-2/Bax.	bufalin	58-65	cytochrome c, somatic	Homo sapiens	171-183
25936899-7	2015	Furthermore, it was shown that SPARC negatively regulated bufalin-induced intrinsic apoptosis by protecting mitochondrial integrity, decreasing the release of cytoplasmic cytochrome c and increasing the ratio of Bcl-2/Bax.	bufalin	58-65	BCL2 apoptosis regulator	Homo sapiens	212-217
25936899-7	2015	Furthermore, it was shown that SPARC negatively regulated bufalin-induced intrinsic apoptosis by protecting mitochondrial integrity, decreasing the release of cytoplasmic cytochrome c and increasing the ratio of Bcl-2/Bax.	bufalin	58-65	BCL2 associated X, apoptosis regulator	Homo sapiens	218-221
25936899-8	2015	In addition, SPARC overcame bufalin-induced G2/M phase arrest by increasing levels of Cyclin B1 and Cyclin A protein expression.	bufalin	28-35	secreted protein acidic and cysteine rich	Homo sapiens	13-18
25936899-8	2015	In addition, SPARC overcame bufalin-induced G2/M phase arrest by increasing levels of Cyclin B1 and Cyclin A protein expression.	bufalin	28-35	cyclin B1	Homo sapiens	86-95
25936899-8	2015	In addition, SPARC overcame bufalin-induced G2/M phase arrest by increasing levels of Cyclin B1 and Cyclin A protein expression.	bufalin	28-35	cyclin A2	Homo sapiens	100-108
25936899-10	2015	This study demonstrated that SPARC antagonizes bufalin-induced apoptosis via inhibition of the intrinsic apoptosis pathway, inhibition of cell cycle arrest and activation of certain pathways involved in proliferation.	bufalin	47-54	secreted protein acidic and cysteine rich	Homo sapiens	29-34
25936899-11	2015	This provides novel evidence for SPARC as a potential target by which to sensitize gastric cancer cells to bufalin.	bufalin	107-114	secreted protein acidic and cysteine rich	Homo sapiens	33-38
26463323-8	2015	The results of transwell migration assay showed that in the presence of HGF, afatinib plus bufalin combination therapy markedly decreased the number of invaded H1975 cells through the Matrigel chamber (48.98 +- 11.43), significantly lower than the 118.92 +- 37.29 of afatinib-treated or the 88.84 +- 19.53 of bufalin-treated cells (P &lt; 0.05 for all).	bufalin	91-98	hepatocyte growth factor	Homo sapiens	72-75
26463323-8	2015	The results of transwell migration assay showed that in the presence of HGF, afatinib plus bufalin combination therapy markedly decreased the number of invaded H1975 cells through the Matrigel chamber (48.98 +- 11.43), significantly lower than the 118.92 +- 37.29 of afatinib-treated or the 88.84 +- 19.53 of bufalin-treated cells (P &lt; 0.05 for all).	bufalin	309-316	hepatocyte growth factor	Homo sapiens	72-75
26463323-10	2015	The results of Western blot analysis showed that the expression of p-EGFR, p-cMet, p-AKT, p-ERK, vimentin and snail in H1975AR cells treated with bufalin puls afatinb was down-regulated markedly, and the expression of E-cadherin was up-regulated markedly.	bufalin	146-153	epidermal growth factor receptor	Homo sapiens	69-73
26463323-10	2015	The results of Western blot analysis showed that the expression of p-EGFR, p-cMet, p-AKT, p-ERK, vimentin and snail in H1975AR cells treated with bufalin puls afatinb was down-regulated markedly, and the expression of E-cadherin was up-regulated markedly.	bufalin	146-153	MET proto-oncogene, receptor tyrosine kinase	Homo sapiens	77-81
26463323-10	2015	The results of Western blot analysis showed that the expression of p-EGFR, p-cMet, p-AKT, p-ERK, vimentin and snail in H1975AR cells treated with bufalin puls afatinb was down-regulated markedly, and the expression of E-cadherin was up-regulated markedly.	bufalin	146-153	AKT serine/threonine kinase 1	Homo sapiens	85-88
26463323-10	2015	The results of Western blot analysis showed that the expression of p-EGFR, p-cMet, p-AKT, p-ERK, vimentin and snail in H1975AR cells treated with bufalin puls afatinb was down-regulated markedly, and the expression of E-cadherin was up-regulated markedly.	bufalin	146-153	eukaryotic translation initiation factor 2 alpha kinase 3	Homo sapiens	90-95
26463323-10	2015	The results of Western blot analysis showed that the expression of p-EGFR, p-cMet, p-AKT, p-ERK, vimentin and snail in H1975AR cells treated with bufalin puls afatinb was down-regulated markedly, and the expression of E-cadherin was up-regulated markedly.	bufalin	146-153	vimentin	Homo sapiens	97-105
26463323-10	2015	The results of Western blot analysis showed that the expression of p-EGFR, p-cMet, p-AKT, p-ERK, vimentin and snail in H1975AR cells treated with bufalin puls afatinb was down-regulated markedly, and the expression of E-cadherin was up-regulated markedly.	bufalin	146-153	snail family transcriptional repressor 1	Homo sapiens	110-115
26463323-10	2015	The results of Western blot analysis showed that the expression of p-EGFR, p-cMet, p-AKT, p-ERK, vimentin and snail in H1975AR cells treated with bufalin puls afatinb was down-regulated markedly, and the expression of E-cadherin was up-regulated markedly.	bufalin	146-153	cadherin 1	Homo sapiens	218-228
26463323-12	2015	The possible mechanism of combined treatment with bufalin and afatinib may be related to the blocking of cMet/PI3K/AKT and cMet/MAPK/ERK pathways and inhibiting of epithelial-mesenchymal transition.	bufalin	50-57	MET proto-oncogene, receptor tyrosine kinase	Homo sapiens	105-109
26463323-12	2015	The possible mechanism of combined treatment with bufalin and afatinib may be related to the blocking of cMet/PI3K/AKT and cMet/MAPK/ERK pathways and inhibiting of epithelial-mesenchymal transition.	bufalin	50-57	AKT serine/threonine kinase 1	Homo sapiens	115-118
26463323-12	2015	The possible mechanism of combined treatment with bufalin and afatinib may be related to the blocking of cMet/PI3K/AKT and cMet/MAPK/ERK pathways and inhibiting of epithelial-mesenchymal transition.	bufalin	50-57	MET proto-oncogene, receptor tyrosine kinase	Homo sapiens	123-127
26463323-12	2015	The possible mechanism of combined treatment with bufalin and afatinib may be related to the blocking of cMet/PI3K/AKT and cMet/MAPK/ERK pathways and inhibiting of epithelial-mesenchymal transition.	bufalin	50-57	mitogen-activated protein kinase 1	Homo sapiens	133-136
25618972-8	2014	Preincubation of 0.001 mumol/L bufalin for 2 h could increase intracellular ADM fluorescence intensity to 28.07% (P &lt; 0.05) and down-regulate MRP1 expression simultaneously, but no remarkable effect was found on P-gp protein.	bufalin	31-38	ATP binding cassette subfamily C member 1	Homo sapiens	145-149
26463323-0	2015	[Bufalin reverses hepatocyte growth factor-induced resistance to afatinib in H1975 lung cancer cells].	bufalin	1-8	hepatocyte growth factor	Homo sapiens	18-42
26463323-1	2015	OBJECTIVE: To investigate the effects of bufalin in reversing hepatocyte growth factor (HGF)-induced resistance to afatinib in H1975 lung cancer cells, and explore its possible mechanism.	bufalin	41-48	hepatocyte growth factor	Homo sapiens	62-86
26463323-1	2015	OBJECTIVE: To investigate the effects of bufalin in reversing hepatocyte growth factor (HGF)-induced resistance to afatinib in H1975 lung cancer cells, and explore its possible mechanism.	bufalin	41-48	hepatocyte growth factor	Homo sapiens	88-91
26463323-6	2015	The expression of EGFR, cMET and EMT signal pathway-related proteins in H1975AR cells treated with bufalin, afatinib and bufalin plus afatinib were detected by Western blot.	bufalin	99-106	epidermal growth factor receptor	Homo sapiens	18-22
26463323-6	2015	The expression of EGFR, cMET and EMT signal pathway-related proteins in H1975AR cells treated with bufalin, afatinib and bufalin plus afatinib were detected by Western blot.	bufalin	99-106	MET proto-oncogene, receptor tyrosine kinase	Homo sapiens	24-28
26463323-6	2015	The expression of EGFR, cMET and EMT signal pathway-related proteins in H1975AR cells treated with bufalin, afatinib and bufalin plus afatinib were detected by Western blot.	bufalin	121-128	epidermal growth factor receptor	Homo sapiens	18-22
26463323-6	2015	The expression of EGFR, cMET and EMT signal pathway-related proteins in H1975AR cells treated with bufalin, afatinib and bufalin plus afatinib were detected by Western blot.	bufalin	121-128	MET proto-oncogene, receptor tyrosine kinase	Homo sapiens	24-28
25653449-16	2015	Src signaling mediated by ATP1A1 was shown to play a crucial role in the inhibition of virus entry by ouabain and bufalin.	bufalin	114-121	SRC proto-oncogene, non-receptor tyrosine kinase	Homo sapiens	0-3
25653449-16	2015	Src signaling mediated by ATP1A1 was shown to play a crucial role in the inhibition of virus entry by ouabain and bufalin.	bufalin	114-121	ATPase Na+/K+ transporting subunit alpha 1	Homo sapiens	26-32
25736684-0	2015	Bufalin is a steroid receptor coactivator inhibitor-response.	bufalin	0-7	steroid receptor RNA activator 1	Homo sapiens	13-41
26064232-0	2015	Bufalin promotes apoptosis of gastric cancer by down-regulation of miR-298 targeting bax.	bufalin	0-7	microRNA 298	Homo sapiens	67-74
26064232-0	2015	Bufalin promotes apoptosis of gastric cancer by down-regulation of miR-298 targeting bax.	bufalin	0-7	BCL2 associated X, apoptosis regulator	Homo sapiens	85-88
26064232-2	2015	Bufalin could induce gastric cancer cell apoptosis via BAX.	bufalin	0-7	BCL2 associated X, apoptosis regulator	Homo sapiens	55-58
26064232-6	2015	Firstly, we verifed that bufalin could induce gastric cancer cell apoptosis by inducing BAX expression.	bufalin	25-32	BCL2 associated X, apoptosis regulator	Homo sapiens	88-91
26064232-11	2015	In summary, bufalin-associated miR-298 may indirectly be involved in cell proliferation and apoptosis by targeting BAX, pointing to use as a potential molecular target in gastric cancer therapy.	bufalin	12-19	microRNA 298	Homo sapiens	31-38
26064232-11	2015	In summary, bufalin-associated miR-298 may indirectly be involved in cell proliferation and apoptosis by targeting BAX, pointing to use as a potential molecular target in gastric cancer therapy.	bufalin	12-19	BCL2 associated X, apoptosis regulator	Homo sapiens	115-118
26053559-10	2015	In summary, CYP3A mediated 5beta-hydroxylation and 3-oxidation were two major metabolic pathways of BF in hepatic microsomes from human and five studied animals, but kinetic analysis demonstrated that the intrinsic clearances of these two metabolic pathways were much different among various species.	bufalin	100-102	cytochrome P450 family 3 subfamily A member 4	Homo sapiens	12-17
25506218-3	2014	Bufalin liposomes (BFL) conjugated with anti-CD40 antibody (anti-CD40-BFL) showed enhanced cytotoxicity compared with bufalin alone.	bufalin	118-125	CD40 molecule	Homo sapiens	45-49
25506218-3	2014	Bufalin liposomes (BFL) conjugated with anti-CD40 antibody (anti-CD40-BFL) showed enhanced cytotoxicity compared with bufalin alone.	bufalin	118-125	CD40 molecule	Homo sapiens	65-69
26446205-0	2015	Bufalin Inhibits NCI-H460 Human Lung Cancer Cell Metastasis In Vitro by Inhibiting MAPKs, MMPs, and NF-kappaB Pathways.	bufalin	0-7	matrix metallopeptidase 2	Homo sapiens	90-94
26446205-0	2015	Bufalin Inhibits NCI-H460 Human Lung Cancer Cell Metastasis In Vitro by Inhibiting MAPKs, MMPs, and NF-kappaB Pathways.	bufalin	0-7	nuclear factor kappa B subunit 1	Homo sapiens	100-109
26446205-4	2015	Bufalin also suppressed the enzymatic activity of matrix metalloproteinase (MMP)-9, which was examined by gelatin zymography methods.	bufalin	0-7	matrix metallopeptidase 9	Homo sapiens	50-82
26446205-6	2015	As evidenced by immunostaining and the electrophoretic mobility shift assay (EMSA), bufalin induced not only a decreased cytoplasmic NF-kappaB production, but also decreased its nuclear translocation.	bufalin	84-91	nuclear factor kappa B subunit 1	Homo sapiens	133-142
26446205-7	2015	Several metastasis-related genes, including Rho-associated (Rho A), coiled-coil-containing protein kinase 1 (ROCK1), and focal adhesion kinase (FAK), were down-regulated after bufalin treatment.	bufalin	176-183	Rho associated coiled-coil containing protein kinase 1	Homo sapiens	109-114
26446205-7	2015	Several metastasis-related genes, including Rho-associated (Rho A), coiled-coil-containing protein kinase 1 (ROCK1), and focal adhesion kinase (FAK), were down-regulated after bufalin treatment.	bufalin	176-183	protein tyrosine kinase 2	Homo sapiens	121-142
26446205-7	2015	Several metastasis-related genes, including Rho-associated (Rho A), coiled-coil-containing protein kinase 1 (ROCK1), and focal adhesion kinase (FAK), were down-regulated after bufalin treatment.	bufalin	176-183	protein tyrosine kinase 2	Homo sapiens	144-147
26111756-0	2015	Bufalin Inhibits the Differentiation and Proliferation of Cancer Stem Cells Derived from Primary Osteosarcoma Cells through Mir-148a.	bufalin	0-7	microRNA 148a	Homo sapiens	124-132
23949904-0	2015	Bufalin inhibits migration and invasion in human hepatocellular carcinoma SK-Hep1 cells through the inhibitions of NF-kB and matrix metalloproteinase-2/-9-signaling pathways.	bufalin	0-7	DNL-type zinc finger	Homo sapiens	77-81
23949904-0	2015	Bufalin inhibits migration and invasion in human hepatocellular carcinoma SK-Hep1 cells through the inhibitions of NF-kB and matrix metalloproteinase-2/-9-signaling pathways.	bufalin	0-7	matrix metallopeptidase 2	Homo sapiens	125-151
23949904-7	2015	Furthermore, bufalin markedly inhibited MMP-2 and -9 activity, mRNA expression and protein levels in SK-Hep1 cells.	bufalin	13-20	matrix metallopeptidase 2	Homo sapiens	40-52
23949904-7	2015	Furthermore, bufalin markedly inhibited MMP-2 and -9 activity, mRNA expression and protein levels in SK-Hep1 cells.	bufalin	13-20	DNL-type zinc finger	Homo sapiens	104-108
23949904-8	2015	Bufalin attenuated phosphoinisitide-3-kinase (PI3K) and phosphorylation of AKT which was associated with reduced levels of nuclear factor kappa B (NF-kappaB).	bufalin	0-7	phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta	Homo sapiens	19-44
23949904-8	2015	Bufalin attenuated phosphoinisitide-3-kinase (PI3K) and phosphorylation of AKT which was associated with reduced levels of nuclear factor kappa B (NF-kappaB).	bufalin	0-7	AKT serine/threonine kinase 1	Homo sapiens	75-78
23949904-8	2015	Bufalin attenuated phosphoinisitide-3-kinase (PI3K) and phosphorylation of AKT which was associated with reduced levels of nuclear factor kappa B (NF-kappaB).	bufalin	0-7	nuclear factor kappa B subunit 1	Homo sapiens	123-145
23949904-8	2015	Bufalin attenuated phosphoinisitide-3-kinase (PI3K) and phosphorylation of AKT which was associated with reduced levels of nuclear factor kappa B (NF-kappaB).	bufalin	0-7	nuclear factor kappa B subunit 1	Homo sapiens	147-156
23949904-9	2015	Bufalin also suppressed protein levels of FAK and Rho A, VEGF, MEKK3, MKK7, and uPA and it diminished NF-kappaB translocation.	bufalin	0-7	protein tyrosine kinase 2	Homo sapiens	42-45
23949904-9	2015	Bufalin also suppressed protein levels of FAK and Rho A, VEGF, MEKK3, MKK7, and uPA and it diminished NF-kappaB translocation.	bufalin	0-7	ras homolog family member A	Homo sapiens	50-55
23949904-9	2015	Bufalin also suppressed protein levels of FAK and Rho A, VEGF, MEKK3, MKK7, and uPA and it diminished NF-kappaB translocation.	bufalin	0-7	vascular endothelial growth factor A	Homo sapiens	57-61
23949904-9	2015	Bufalin also suppressed protein levels of FAK and Rho A, VEGF, MEKK3, MKK7, and uPA and it diminished NF-kappaB translocation.	bufalin	0-7	mitogen-activated protein kinase kinase kinase 3	Homo sapiens	63-68
23949904-9	2015	Bufalin also suppressed protein levels of FAK and Rho A, VEGF, MEKK3, MKK7, and uPA and it diminished NF-kappaB translocation.	bufalin	0-7	mitogen-activated protein kinase kinase 7	Homo sapiens	70-74
23949904-9	2015	Bufalin also suppressed protein levels of FAK and Rho A, VEGF, MEKK3, MKK7, and uPA and it diminished NF-kappaB translocation.	bufalin	0-7	proline rich acidic protein 1	Homo sapiens	80-83
23949904-9	2015	Bufalin also suppressed protein levels of FAK and Rho A, VEGF, MEKK3, MKK7, and uPA and it diminished NF-kappaB translocation.	bufalin	0-7	nuclear factor kappa B subunit 1	Homo sapiens	102-111
23949904-10	2015	Based on these observations, we propose that bufalin is acts as an antiinvasive agent by inhibiting MMP-2 and -9 and involving PI3K/AKT and NF-kappaB pathways.	bufalin	45-52	matrix metallopeptidase 2	Homo sapiens	100-112
23949904-10	2015	Based on these observations, we propose that bufalin is acts as an antiinvasive agent by inhibiting MMP-2 and -9 and involving PI3K/AKT and NF-kappaB pathways.	bufalin	45-52	AKT serine/threonine kinase 1	Homo sapiens	132-135
23949904-10	2015	Based on these observations, we propose that bufalin is acts as an antiinvasive agent by inhibiting MMP-2 and -9 and involving PI3K/AKT and NF-kappaB pathways.	bufalin	45-52	nuclear factor kappa B subunit 1	Homo sapiens	140-149
26783410-0	2015	Bufalin Induces Mitochondria-Dependent Apoptosis in Pancreatic and Oral Cancer Cells by Downregulating hTERT Expression via Activation of the JNK/p38 Pathway.	bufalin	0-7	telomerase reverse transcriptase	Homo sapiens	103-108
26783410-0	2015	Bufalin Induces Mitochondria-Dependent Apoptosis in Pancreatic and Oral Cancer Cells by Downregulating hTERT Expression via Activation of the JNK/p38 Pathway.	bufalin	0-7	mitogen-activated protein kinase 14	Homo sapiens	146-149
26783410-8	2015	Collectively, these data suggest that bufalin downregulates hTERT to induce mitochondria-dependent apoptosis in CAPAN-2 and CAL-27 cells.	bufalin	38-45	telomerase reverse transcriptase	Homo sapiens	60-65
26783410-9	2015	Moreover, bufalin increased the phosphorylation of JNK and p38-MAPK in CAPAN-2 and CAL-27 cells, and blocking the JNK/p38-MAPK pathway using the JNK inhibitor SP600125 or the p38-MAPK inhibitor SB203580 reversed bufalin-induced hTERT downregulation.	bufalin	10-17	mitogen-activated protein kinase 14	Homo sapiens	59-62
26783410-9	2015	Moreover, bufalin increased the phosphorylation of JNK and p38-MAPK in CAPAN-2 and CAL-27 cells, and blocking the JNK/p38-MAPK pathway using the JNK inhibitor SP600125 or the p38-MAPK inhibitor SB203580 reversed bufalin-induced hTERT downregulation.	bufalin	10-17	mitogen-activated protein kinase 14	Homo sapiens	118-121
26783410-9	2015	Moreover, bufalin increased the phosphorylation of JNK and p38-MAPK in CAPAN-2 and CAL-27 cells, and blocking the JNK/p38-MAPK pathway using the JNK inhibitor SP600125 or the p38-MAPK inhibitor SB203580 reversed bufalin-induced hTERT downregulation.	bufalin	10-17	mitogen-activated protein kinase 14	Homo sapiens	118-121
26783410-9	2015	Moreover, bufalin increased the phosphorylation of JNK and p38-MAPK in CAPAN-2 and CAL-27 cells, and blocking the JNK/p38-MAPK pathway using the JNK inhibitor SP600125 or the p38-MAPK inhibitor SB203580 reversed bufalin-induced hTERT downregulation.	bufalin	10-17	telomerase reverse transcriptase	Homo sapiens	228-233
26783410-10	2015	Thus, the JNK/p38 pathway is involved in bufalin-induced hTERT downregulation and subsequent induction of apoptosis by the mitochondrial pathway.	bufalin	41-48	mitogen-activated protein kinase 14	Homo sapiens	14-17
26783410-10	2015	Thus, the JNK/p38 pathway is involved in bufalin-induced hTERT downregulation and subsequent induction of apoptosis by the mitochondrial pathway.	bufalin	41-48	telomerase reverse transcriptase	Homo sapiens	57-62
26770191-0	2015	Bufalin Inhibits HCT116 Colon Cancer Cells and Its Orthotopic Xenograft Tumor in Mice Model through Genes Related to Apoptotic and PTEN/AKT Pathways.	bufalin	0-7	phosphatase and tensin homolog	Mus musculus	131-135
26770191-0	2015	Bufalin Inhibits HCT116 Colon Cancer Cells and Its Orthotopic Xenograft Tumor in Mice Model through Genes Related to Apoptotic and PTEN/AKT Pathways.	bufalin	0-7	thymoma viral proto-oncogene 1	Mus musculus	136-139
26770191-11	2015	In the Bufalin-treated cultured cells and/or xenograft tumors, the expressions of PTEN, Bad, Bax, and Caspase-3 were significantly increased, while p-AKT and Bcl-xL significantly decreased.	bufalin	7-14	phosphatase and tensin homolog	Homo sapiens	82-86
26770191-11	2015	In the Bufalin-treated cultured cells and/or xenograft tumors, the expressions of PTEN, Bad, Bax, and Caspase-3 were significantly increased, while p-AKT and Bcl-xL significantly decreased.	bufalin	7-14	BCL2 associated X, apoptosis regulator	Homo sapiens	93-96
26770191-11	2015	In the Bufalin-treated cultured cells and/or xenograft tumors, the expressions of PTEN, Bad, Bax, and Caspase-3 were significantly increased, while p-AKT and Bcl-xL significantly decreased.	bufalin	7-14	caspase 3	Homo sapiens	102-111
26770191-11	2015	In the Bufalin-treated cultured cells and/or xenograft tumors, the expressions of PTEN, Bad, Bax, and Caspase-3 were significantly increased, while p-AKT and Bcl-xL significantly decreased.	bufalin	7-14	AKT serine/threonine kinase 1	Homo sapiens	150-153
26770191-11	2015	In the Bufalin-treated cultured cells and/or xenograft tumors, the expressions of PTEN, Bad, Bax, and Caspase-3 were significantly increased, while p-AKT and Bcl-xL significantly decreased.	bufalin	7-14	BCL2 like 1	Homo sapiens	158-164
25618972-8	2014	Preincubation of 0.001 mumol/L bufalin for 2 h could increase intracellular ADM fluorescence intensity to 28.07% (P &lt; 0.05) and down-regulate MRP1 expression simultaneously, but no remarkable effect was found on P-gp protein.	bufalin	31-38	ATP binding cassette subfamily B member 1	Homo sapiens	215-219
25085580-5	2014	Activation of caspase-9 and the subsequent activation of caspase-3 indicated that bufalin may be inducing mitochondria apoptosis pathways.	bufalin	82-89	caspase 9	Homo sapiens	14-23
25085580-5	2014	Activation of caspase-9 and the subsequent activation of caspase-3 indicated that bufalin may be inducing mitochondria apoptosis pathways.	bufalin	82-89	caspase 3	Homo sapiens	57-66
24710190-0	2014	Bufalin enhances TRAIL-induced apoptosis by redistributing death receptors in lipid rafts in breast cancer cells.	bufalin	0-7	TNF superfamily member 10	Homo sapiens	17-22
24752615-0	2014	Bufalin exerts inhibitory effects on IL-1beta-mediated proliferation and induces apoptosis in human rheumatoid arthritis fibroblast-like synoviocytes.	bufalin	0-7	interleukin 1 beta	Homo sapiens	37-45
24752615-3	2014	In this study, we explored the effects of bufalin on interleukin-1beta (IL-1beta)-induced proliferation and apoptosis of RAFLSs.	bufalin	42-49	interleukin 1 beta	Homo sapiens	53-70
24752615-3	2014	In this study, we explored the effects of bufalin on interleukin-1beta (IL-1beta)-induced proliferation and apoptosis of RAFLSs.	bufalin	42-49	interleukin 1 beta	Homo sapiens	72-80
24752615-5	2014	Bufalin dose-dependently inhibited IL-1beta-induced RAFLS proliferation.	bufalin	0-7	interleukin 1 beta	Homo sapiens	35-43
24752615-6	2014	Mechanistically, bufalin decreased the activation of mitogen-activated protein kinases (MAPKs) and nuclear factor-kappa B (NF-kappaB), both of which are involved in IL-1beta-mediated RAFLS proliferation.	bufalin	17-24	nuclear factor kappa B subunit 1	Homo sapiens	99-121
24752615-6	2014	Mechanistically, bufalin decreased the activation of mitogen-activated protein kinases (MAPKs) and nuclear factor-kappa B (NF-kappaB), both of which are involved in IL-1beta-mediated RAFLS proliferation.	bufalin	17-24	nuclear factor kappa B subunit 1	Homo sapiens	123-132
24752615-6	2014	Mechanistically, bufalin decreased the activation of mitogen-activated protein kinases (MAPKs) and nuclear factor-kappa B (NF-kappaB), both of which are involved in IL-1beta-mediated RAFLS proliferation.	bufalin	17-24	interleukin 1 beta	Homo sapiens	165-173
24752615-7	2014	Moreover, bufalin induced apoptosis and mitochondrial damage of RAFLSs, which was associated with Bcl-2 downregulation, Bax upregulation, mitochondrial cytochrome c release, and enhanced cleavages of caspase-3 and poly-(ADP-ribose) polymerase.	bufalin	10-17	BCL2 apoptosis regulator	Homo sapiens	98-103
24752615-7	2014	Moreover, bufalin induced apoptosis and mitochondrial damage of RAFLSs, which was associated with Bcl-2 downregulation, Bax upregulation, mitochondrial cytochrome c release, and enhanced cleavages of caspase-3 and poly-(ADP-ribose) polymerase.	bufalin	10-17	BCL2 associated X, apoptosis regulator	Homo sapiens	120-123
24752615-7	2014	Moreover, bufalin induced apoptosis and mitochondrial damage of RAFLSs, which was associated with Bcl-2 downregulation, Bax upregulation, mitochondrial cytochrome c release, and enhanced cleavages of caspase-3 and poly-(ADP-ribose) polymerase.	bufalin	10-17	cytochrome c, somatic	Homo sapiens	152-164
24752615-7	2014	Moreover, bufalin induced apoptosis and mitochondrial damage of RAFLSs, which was associated with Bcl-2 downregulation, Bax upregulation, mitochondrial cytochrome c release, and enhanced cleavages of caspase-3 and poly-(ADP-ribose) polymerase.	bufalin	10-17	caspase 3	Homo sapiens	200-242
24752615-8	2014	Collectively, our results reveal that bufalin suppresses IL-1beta-induced proliferation of RAFLSs through MAPK and NF-kappaB signaling pathways and induces RAFLS apoptosis via the mitochondria-dependent pathway.	bufalin	38-45	interleukin 1 beta	Homo sapiens	57-65
24752615-8	2014	Collectively, our results reveal that bufalin suppresses IL-1beta-induced proliferation of RAFLSs through MAPK and NF-kappaB signaling pathways and induces RAFLS apoptosis via the mitochondria-dependent pathway.	bufalin	38-45	nuclear factor kappa B subunit 1	Homo sapiens	115-124
24515724-6	2014	Our results revealed that TNF-alpha significantly increased p65 translocation into nucleus (P &lt; 0.01) and enhanced NF-kappaB DNA-binding activity, which were dose-dependently inhibited by bufalin.	bufalin	191-198	tumor necrosis factor	Homo sapiens	26-35
24515724-6	2014	Our results revealed that TNF-alpha significantly increased p65 translocation into nucleus (P &lt; 0.01) and enhanced NF-kappaB DNA-binding activity, which were dose-dependently inhibited by bufalin.	bufalin	191-198	RELA proto-oncogene, NF-kB subunit	Homo sapiens	60-63
24515724-6	2014	Our results revealed that TNF-alpha significantly increased p65 translocation into nucleus (P &lt; 0.01) and enhanced NF-kappaB DNA-binding activity, which were dose-dependently inhibited by bufalin.	bufalin	191-198	nuclear factor kappa B subunit 1	Homo sapiens	118-127
24515724-7	2014	Furthermore, bufalin attenuated the TNF-alpha-induced interleukin-1beta (IL-1beta), IL-6, and IL-8 production in RAFLSs in a concentration-dependent manner.	bufalin	13-20	tumor necrosis factor	Homo sapiens	36-45
24515724-7	2014	Furthermore, bufalin attenuated the TNF-alpha-induced interleukin-1beta (IL-1beta), IL-6, and IL-8 production in RAFLSs in a concentration-dependent manner.	bufalin	13-20	interleukin 1 beta	Homo sapiens	54-71
24515724-7	2014	Furthermore, bufalin attenuated the TNF-alpha-induced interleukin-1beta (IL-1beta), IL-6, and IL-8 production in RAFLSs in a concentration-dependent manner.	bufalin	13-20	interleukin 1 beta	Homo sapiens	73-81
24515724-7	2014	Furthermore, bufalin attenuated the TNF-alpha-induced interleukin-1beta (IL-1beta), IL-6, and IL-8 production in RAFLSs in a concentration-dependent manner.	bufalin	13-20	interleukin 6	Homo sapiens	84-88
24515724-7	2014	Furthermore, bufalin attenuated the TNF-alpha-induced interleukin-1beta (IL-1beta), IL-6, and IL-8 production in RAFLSs in a concentration-dependent manner.	bufalin	13-20	C-X-C motif chemokine ligand 8	Homo sapiens	94-98
24515724-8	2014	Interestingly, TNF-alpha-induced invasion of RAFLSs was dampened by the pretreatment of bufalin.	bufalin	88-95	tumor necrosis factor	Homo sapiens	15-24
24515724-10	2014	Our results reveal that bufalin can inhibit TNF-alpha-induced NF-kappaB activation, cytokine production, invasion, and MMP-9 expression in RAFLSs, indicating a therapeutic potential of bufalin on RA.	bufalin	24-31	tumor necrosis factor	Homo sapiens	44-53
24515724-10	2014	Our results reveal that bufalin can inhibit TNF-alpha-induced NF-kappaB activation, cytokine production, invasion, and MMP-9 expression in RAFLSs, indicating a therapeutic potential of bufalin on RA.	bufalin	24-31	nuclear factor kappa B subunit 1	Homo sapiens	62-71
24515724-10	2014	Our results reveal that bufalin can inhibit TNF-alpha-induced NF-kappaB activation, cytokine production, invasion, and MMP-9 expression in RAFLSs, indicating a therapeutic potential of bufalin on RA.	bufalin	24-31	matrix metallopeptidase 9	Homo sapiens	119-124
24710190-4	2014	The results showed that bufalin enhanced TRAIL-induced apoptosis in MCF-7 and MDA-MB-231 breast cancer cells by activating the extrinsic apoptotic pathway.	bufalin	24-31	TNF superfamily member 10	Homo sapiens	41-46
24710190-5	2014	Bufalin also promoted the clustering of death receptor 4 (DR4) and DR5 in aggregated lipid rafts.	bufalin	0-7	TNF receptor superfamily member 10a	Homo sapiens	40-56
24710190-5	2014	Bufalin also promoted the clustering of death receptor 4 (DR4) and DR5 in aggregated lipid rafts.	bufalin	0-7	TNF receptor superfamily member 10a	Homo sapiens	58-61
24710190-5	2014	Bufalin also promoted the clustering of death receptor 4 (DR4) and DR5 in aggregated lipid rafts.	bufalin	0-7	TNF receptor superfamily member 10b	Homo sapiens	67-70
24710190-7	2014	Overall, these results indicate that bufalin enhanced TRAIL-induced apoptosis in breast cancer cells by the partial redistribution of DRs in lipid rafts.	bufalin	37-44	TNF superfamily member 10	Homo sapiens	54-59
25272506-2	2014	METHOD: MTT assay was used to detect the growth inhibition rates of osteosarcoma cells U-20S, U-20S/MTX300, SaOS-2, IOR/OS9 treated with bufalin in different concentrations and times.	bufalin	137-144	OS9 endoplasmic reticulum lectin	Homo sapiens	120-123
25272506-6	2014	RESULT: Bufalin inhibited the growth of human osteosarcoma cells U20S, U20S/MTX300 (methotrexate resistant cells), SAOS2, IOR/OS9 in a dose- and time-dependent manner.	bufalin	8-15	OS9 endoplasmic reticulum lectin	Homo sapiens	126-129
24710190-3	2014	In the present study, we explored the effect of bufalin on TRAIL-induced breast cancer cell apoptosis.	bufalin	48-55	TNF superfamily member 10	Homo sapiens	59-64
24566825-7	2014	Bufalin is able to downregulate BMI-1 expression levels in K562 cells prematurely and cause an increase in the expression levels of p16INK4a and p14ARF.	bufalin	0-7	BMI1 proto-oncogene, polycomb ring finger	Homo sapiens	32-37
25272506-11	2014	Ectopic expression of Hsp27 could reduce the bufalin-induced apoptosis remarkably in U20S and U20S/MTX300 cells.	bufalin	45-52	heat shock protein family B (small) member 1	Homo sapiens	22-27
25272506-14	2014	Among them, downregulation of Hsp27 plays a critical role in the bufalin-induced apoptosis in human osteosarcoma cells.	bufalin	65-72	heat shock protein family B (small) member 1	Homo sapiens	30-35
24570183-0	2014	Bufalin inhibited the growth of human osteosarcoma MG-63 cells via down-regulation of Bcl-2/Bax and triggering of the mitochondrial pathway.	bufalin	0-7	BCL2 apoptosis regulator	Homo sapiens	86-91
24570183-0	2014	Bufalin inhibited the growth of human osteosarcoma MG-63 cells via down-regulation of Bcl-2/Bax and triggering of the mitochondrial pathway.	bufalin	0-7	BCL2 associated X, apoptosis regulator	Homo sapiens	92-95
24570183-6	2014	By western blot analysis, we found that the up-regulation of Apaf-1, cleaved PARP, cleaved caspase-3, cleaved caspase-9, and Bax/Bcl-2, varies with different concentration of Bufalin.	bufalin	175-182	apoptotic peptidase activating factor 1	Homo sapiens	61-67
24570183-6	2014	By western blot analysis, we found that the up-regulation of Apaf-1, cleaved PARP, cleaved caspase-3, cleaved caspase-9, and Bax/Bcl-2, varies with different concentration of Bufalin.	bufalin	175-182	caspase 9	Homo sapiens	110-119
24570183-6	2014	By western blot analysis, we found that the up-regulation of Apaf-1, cleaved PARP, cleaved caspase-3, cleaved caspase-9, and Bax/Bcl-2, varies with different concentration of Bufalin.	bufalin	175-182	BCL2 apoptosis regulator	Homo sapiens	129-134
24566825-7	2014	Bufalin is able to downregulate BMI-1 expression levels in K562 cells prematurely and cause an increase in the expression levels of p16INK4a and p14ARF.	bufalin	0-7	cyclin dependent kinase inhibitor 2A	Homo sapiens	132-140
24566825-7	2014	Bufalin is able to downregulate BMI-1 expression levels in K562 cells prematurely and cause an increase in the expression levels of p16INK4a and p14ARF.	bufalin	0-7	cyclin dependent kinase inhibitor 2A	Homo sapiens	145-151
24566825-9	2014	Bufalin may induce K562 cell apoptosis by downregulating BMI-1 expression levels and accordingly upregulating the expression levels of p16INK4a and p14ARF.	bufalin	0-7	BMI1 proto-oncogene, polycomb ring finger	Homo sapiens	57-62
24566825-9	2014	Bufalin may induce K562 cell apoptosis by downregulating BMI-1 expression levels and accordingly upregulating the expression levels of p16INK4a and p14ARF.	bufalin	0-7	cyclin dependent kinase inhibitor 2A	Homo sapiens	135-143
24566825-9	2014	Bufalin may induce K562 cell apoptosis by downregulating BMI-1 expression levels and accordingly upregulating the expression levels of p16INK4a and p14ARF.	bufalin	0-7	cyclin dependent kinase inhibitor 2A	Homo sapiens	148-154
24566825-10	2014	Bufalin may also induce K562 cell apoptosis via downregulating BCR/ABL expression levels, and this pathway may be independent of the BMI-1 pathway.	bufalin	0-7	ABL proto-oncogene 1, non-receptor tyrosine kinase	Homo sapiens	63-70
24211617-1	2014	Molecular docking studies have shown that Delta(8,14)-anhydrobufalin (1) exhibited more potent binding affinity on androgen receptor (AR) than Delta(14,15)-anhydrobufalin (2) and bufalin (3).	bufalin	61-68	androgen receptor	Homo sapiens	115-132
24550689-7	2014	In addition, bufalin was also found to induce ER stress-mediated apoptosis, which was supported by the up- regulation of ER stress markers, CHOP and GRP78, and augmented phosphorylation of PERK and eIF2alpha as well as cleavage of caspase-4.	bufalin	13-20	DNA damage inducible transcript 3	Homo sapiens	140-144
24550689-7	2014	In addition, bufalin was also found to induce ER stress-mediated apoptosis, which was supported by the up- regulation of ER stress markers, CHOP and GRP78, and augmented phosphorylation of PERK and eIF2alpha as well as cleavage of caspase-4.	bufalin	13-20	heat shock protein family A (Hsp70) member 5	Homo sapiens	149-154
24550689-7	2014	In addition, bufalin was also found to induce ER stress-mediated apoptosis, which was supported by the up- regulation of ER stress markers, CHOP and GRP78, and augmented phosphorylation of PERK and eIF2alpha as well as cleavage of caspase-4.	bufalin	13-20	eukaryotic translation initiation factor 2 alpha kinase 3	Homo sapiens	189-193
24550689-7	2014	In addition, bufalin was also found to induce ER stress-mediated apoptosis, which was supported by the up- regulation of ER stress markers, CHOP and GRP78, and augmented phosphorylation of PERK and eIF2alpha as well as cleavage of caspase-4.	bufalin	13-20	eukaryotic translation initiation factor 2A	Homo sapiens	198-207
24550689-7	2014	In addition, bufalin was also found to induce ER stress-mediated apoptosis, which was supported by the up- regulation of ER stress markers, CHOP and GRP78, and augmented phosphorylation of PERK and eIF2alpha as well as cleavage of caspase-4.	bufalin	13-20	caspase 4	Homo sapiens	231-240
24550689-10	2014	Further experiments showed that the mechanism of bufalin-induced autophagy associated with ATP deleption involved an increase in the active form of AMPK, decreased phosphorylation levels of mTOR and its downstream targets 4EBP1 and p70S6K1.	bufalin	49-56	mechanistic target of rapamycin kinase	Homo sapiens	190-194
24550689-10	2014	Further experiments showed that the mechanism of bufalin-induced autophagy associated with ATP deleption involved an increase in the active form of AMPK, decreased phosphorylation levels of mTOR and its downstream targets 4EBP1 and p70S6K1.	bufalin	49-56	eukaryotic translation initiation factor 4E binding protein 1	Homo sapiens	222-227
24550689-11	2014	Furthermore, TUDC and silencing of eIF2alpha or CHOP partially blocked bufalin-induced accumulation of LC3-II, which indicated that ER stress preceded bufalin-induced autophagy and PERK/eIF2alpha/CHOP signaling pathway played a major part in the process.	bufalin	71-78	eukaryotic translation initiation factor 2A	Homo sapiens	35-44
24550689-11	2014	Furthermore, TUDC and silencing of eIF2alpha or CHOP partially blocked bufalin-induced accumulation of LC3-II, which indicated that ER stress preceded bufalin-induced autophagy and PERK/eIF2alpha/CHOP signaling pathway played a major part in the process.	bufalin	71-78	DNA damage inducible transcript 3	Homo sapiens	48-52
24550689-11	2014	Furthermore, TUDC and silencing of eIF2alpha or CHOP partially blocked bufalin-induced accumulation of LC3-II, which indicated that ER stress preceded bufalin-induced autophagy and PERK/eIF2alpha/CHOP signaling pathway played a major part in the process.	bufalin	71-78	eukaryotic translation initiation factor 2 alpha kinase 3	Homo sapiens	181-185
24550689-11	2014	Furthermore, TUDC and silencing of eIF2alpha or CHOP partially blocked bufalin-induced accumulation of LC3-II, which indicated that ER stress preceded bufalin-induced autophagy and PERK/eIF2alpha/CHOP signaling pathway played a major part in the process.	bufalin	71-78	eukaryotic translation initiation factor 2A	Homo sapiens	186-195
24550689-11	2014	Furthermore, TUDC and silencing of eIF2alpha or CHOP partially blocked bufalin-induced accumulation of LC3-II, which indicated that ER stress preceded bufalin-induced autophagy and PERK/eIF2alpha/CHOP signaling pathway played a major part in the process.	bufalin	71-78	DNA damage inducible transcript 3	Homo sapiens	196-200
24550689-11	2014	Furthermore, TUDC and silencing of eIF2alpha or CHOP partially blocked bufalin-induced accumulation of LC3-II, which indicated that ER stress preceded bufalin-induced autophagy and PERK/eIF2alpha/CHOP signaling pathway played a major part in the process.	bufalin	151-158	eukaryotic translation initiation factor 2A	Homo sapiens	35-44
24550689-11	2014	Furthermore, TUDC and silencing of eIF2alpha or CHOP partially blocked bufalin-induced accumulation of LC3-II, which indicated that ER stress preceded bufalin-induced autophagy and PERK/eIF2alpha/CHOP signaling pathway played a major part in the process.	bufalin	151-158	DNA damage inducible transcript 3	Homo sapiens	196-200
24006225-12	2014	When treated with bufalin, the sphere could not get attached to the flask and failed to differentiate, which was indicated by the stable expression of stem cell marker CD133 and OCT-4 in the condition permissive to differentiation.	bufalin	18-25	prominin 1	Homo sapiens	168-173
24006225-12	2014	When treated with bufalin, the sphere could not get attached to the flask and failed to differentiate, which was indicated by the stable expression of stem cell marker CD133 and OCT-4 in the condition permissive to differentiation.	bufalin	18-25	POU class 5 homeobox 1	Homo sapiens	178-183
24390736-0	2014	Bufalin is a potent small-molecule inhibitor of the steroid receptor coactivators SRC-3 and SRC-1.	bufalin	0-7	nuclear receptor coactivator 3	Mus musculus	82-87
24390736-0	2014	Bufalin is a potent small-molecule inhibitor of the steroid receptor coactivators SRC-3 and SRC-1.	bufalin	0-7	nuclear receptor coactivator 1	Mus musculus	92-97
24390736-4	2014	Here, we report on the results of a high-throughput screening effort that identified the cardiac glycoside bufalin as a potent small-molecule inhibitor for SRC-3 and SRC-1.	bufalin	107-114	nuclear receptor coactivator 3	Mus musculus	156-161
24390736-4	2014	Here, we report on the results of a high-throughput screening effort that identified the cardiac glycoside bufalin as a potent small-molecule inhibitor for SRC-3 and SRC-1.	bufalin	107-114	nuclear receptor coactivator 1	Mus musculus	166-171
24390736-5	2014	Bufalin strongly promoted SRC-3 protein degradation and was able to block cancer cell growth at nanomolar concentrations.	bufalin	0-7	nuclear receptor coactivator 3	Mus musculus	26-31
24218335-9	2014	With western blotting, we found that the expression level of an antiapoptotic protein heat shock protein 27 (Hsp27) and its partner molecule p-Akt was decreased upon the treatment with bufalin.	bufalin	185-192	heat shock protein family B (small) member 1	Homo sapiens	86-107
24218335-9	2014	With western blotting, we found that the expression level of an antiapoptotic protein heat shock protein 27 (Hsp27) and its partner molecule p-Akt was decreased upon the treatment with bufalin.	bufalin	185-192	heat shock protein family B (small) member 1	Homo sapiens	109-114
24218335-12	2014	The influence on the level of cylinB1, CDK1, and p21 was also observed after bufalin treatment, and the relationship between Hsp27 and the cell cycle-related proteins mentioned above deserves much more research.	bufalin	77-84	cyclin dependent kinase 1	Homo sapiens	39-43
24218335-12	2014	The influence on the level of cylinB1, CDK1, and p21 was also observed after bufalin treatment, and the relationship between Hsp27 and the cell cycle-related proteins mentioned above deserves much more research.	bufalin	77-84	H3 histone pseudogene 16	Homo sapiens	49-52
24218335-12	2014	The influence on the level of cylinB1, CDK1, and p21 was also observed after bufalin treatment, and the relationship between Hsp27 and the cell cycle-related proteins mentioned above deserves much more research.	bufalin	77-84	heat shock protein family B (small) member 1	Homo sapiens	125-130
24375248-2	2014	The expression of miR-497 in colorectal cancer cells with prior treatment with bufalin was determined using real-time quantitative PCR.	bufalin	79-86	microRNA 497	Homo sapiens	18-25
24375248-6	2014	This study revealed that bufalin inhibited angiogenesis and regulated miR-497 expression and that bufalin and miR-497 acted in synergy to inhibit colorectal cancer metastasis, resulting in improved quality of life in a nude mouse model.	bufalin	25-32	microRNA 497	Mus musculus	70-77
24581171-10	2014	Certain changes of expression of AKT/GSK3beta/beta-catenin/E-cadherin signaling pathway-related proteins were in tumor tissues, which were related to the bufalin dose.	bufalin	154-161	AKT serine/threonine kinase 1	Homo sapiens	33-36
24581171-10	2014	Certain changes of expression of AKT/GSK3beta/beta-catenin/E-cadherin signaling pathway-related proteins were in tumor tissues, which were related to the bufalin dose.	bufalin	154-161	glycogen synthase kinase 3 beta	Homo sapiens	37-45
24581171-10	2014	Certain changes of expression of AKT/GSK3beta/beta-catenin/E-cadherin signaling pathway-related proteins were in tumor tissues, which were related to the bufalin dose.	bufalin	154-161	catenin beta 1	Homo sapiens	46-58
24581171-10	2014	Certain changes of expression of AKT/GSK3beta/beta-catenin/E-cadherin signaling pathway-related proteins were in tumor tissues, which were related to the bufalin dose.	bufalin	154-161	cadherin 1	Homo sapiens	59-69
24581171-13	2014	Inhibition of AKT/GSK3beta/beta-catenin/E-cadherin signaling pathways by bufalin may show therapeutic effects in advanced HCC patients.	bufalin	73-80	AKT serine/threonine kinase 1	Homo sapiens	14-17
24581171-13	2014	Inhibition of AKT/GSK3beta/beta-catenin/E-cadherin signaling pathways by bufalin may show therapeutic effects in advanced HCC patients.	bufalin	73-80	glycogen synthase kinase 3 beta	Homo sapiens	18-26
24581171-13	2014	Inhibition of AKT/GSK3beta/beta-catenin/E-cadherin signaling pathways by bufalin may show therapeutic effects in advanced HCC patients.	bufalin	73-80	catenin beta 1	Homo sapiens	27-39
24581171-13	2014	Inhibition of AKT/GSK3beta/beta-catenin/E-cadherin signaling pathways by bufalin may show therapeutic effects in advanced HCC patients.	bufalin	73-80	cadherin 1	Homo sapiens	40-50
24211617-1	2014	Molecular docking studies have shown that Delta(8,14)-anhydrobufalin (1) exhibited more potent binding affinity on androgen receptor (AR) than Delta(14,15)-anhydrobufalin (2) and bufalin (3).	bufalin	61-68	androgen receptor	Homo sapiens	134-136
24267199-8	2013	In prostate cancer PC-3cell line, bufalin-induced apoptosis can be largely attenuated by a miR-181a inhibitor, which blocked bufalin-induced Bcl-2 reduction and caspase-3 activation.	bufalin	34-41	caspase 3	Homo sapiens	161-170
24871662-5	2014	Bufalin could activate phosphorylated p53 in NCI-H460 cells.	bufalin	0-7	tumor protein p53	Homo sapiens	38-41
24114361-0	2014	Blocking autophagy enhances the apoptosis effect of bufalin on human hepatocellular carcinoma cells through endoplasmic reticulum stress and JNK activation.	bufalin	52-59	mitogen-activated protein kinase 8	Homo sapiens	141-144
24114361-4	2014	The underlying mechanism of the bufalin-induced apoptosis was the induction of endoplasmic reticulum (ER) stress via the IRE1-JNK pathway.	bufalin	32-39	endoplasmic reticulum to nucleus signaling 1	Homo sapiens	121-125
24114361-4	2014	The underlying mechanism of the bufalin-induced apoptosis was the induction of endoplasmic reticulum (ER) stress via the IRE1-JNK pathway.	bufalin	32-39	mitogen-activated protein kinase 8	Homo sapiens	126-129
24114361-6	2014	Our data supported the pro-survival role of bufalin-induced autophagy when the autophagy pathway was blocked with specific chemical inhibitors; the involvement of the IRE1 pathway in the ER stress-induced autophagy was also demonstrated when the expression of IRE1 and CHOP was silenced using siRNA.	bufalin	44-51	endoplasmic reticulum to nucleus signaling 1	Homo sapiens	260-264
24114361-6	2014	Our data supported the pro-survival role of bufalin-induced autophagy when the autophagy pathway was blocked with specific chemical inhibitors; the involvement of the IRE1 pathway in the ER stress-induced autophagy was also demonstrated when the expression of IRE1 and CHOP was silenced using siRNA.	bufalin	44-51	DNA damage inducible transcript 3	Homo sapiens	269-273
21922632-0	2014	Bufalin-inhibited migration and invasion in human osteosarcoma U-2 OS cells is carried out by suppression of the matrix metalloproteinase-2, ERK, and JNK signaling pathways.	bufalin	0-7	matrix metallopeptidase 2	Homo sapiens	113-139
21922632-0	2014	Bufalin-inhibited migration and invasion in human osteosarcoma U-2 OS cells is carried out by suppression of the matrix metalloproteinase-2, ERK, and JNK signaling pathways.	bufalin	0-7	mitogen-activated protein kinase 1	Homo sapiens	141-144
21922632-0	2014	Bufalin-inhibited migration and invasion in human osteosarcoma U-2 OS cells is carried out by suppression of the matrix metalloproteinase-2, ERK, and JNK signaling pathways.	bufalin	0-7	mitogen-activated protein kinase 8	Homo sapiens	150-153
21922632-8	2014	Bufalin also suppressed the protein level of MMP-2 and reduced the levels of mitogen-activated protein kinases (MAPKs) such as JNK1/2 and ERK1/2 signals in U-2 OS cells.	bufalin	0-7	matrix metallopeptidase 2	Homo sapiens	45-50
21922632-8	2014	Bufalin also suppressed the protein level of MMP-2 and reduced the levels of mitogen-activated protein kinases (MAPKs) such as JNK1/2 and ERK1/2 signals in U-2 OS cells.	bufalin	0-7	mitogen-activated protein kinase 8	Homo sapiens	127-133
21922632-8	2014	Bufalin also suppressed the protein level of MMP-2 and reduced the levels of mitogen-activated protein kinases (MAPKs) such as JNK1/2 and ERK1/2 signals in U-2 OS cells.	bufalin	0-7	mitogen-activated protein kinase 3	Homo sapiens	138-144
21922632-9	2014	Our results suggest that signaling pathways for bufalin-inhibited migration and invasion of U-2 OS cells might be mediated through blocking MAPK signaling and resulting in the inhibition of MMP-2.	bufalin	48-55	mitogen-activated protein kinase 3	Homo sapiens	140-144
21922632-9	2014	Our results suggest that signaling pathways for bufalin-inhibited migration and invasion of U-2 OS cells might be mediated through blocking MAPK signaling and resulting in the inhibition of MMP-2.	bufalin	48-55	matrix metallopeptidase 2	Homo sapiens	190-195
24719521-6	2014	Bufalin down regulated the expression levels of nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) during these treatments.	bufalin	0-7	nitric oxide synthase 2	Homo sapiens	71-75
24719521-6	2014	Bufalin down regulated the expression levels of nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) during these treatments.	bufalin	0-7	prostaglandin-endoperoxide synthase 2	Homo sapiens	78-94
24719521-6	2014	Bufalin down regulated the expression levels of nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) during these treatments.	bufalin	0-7	prostaglandin-endoperoxide synthase 2	Homo sapiens	96-101
24719521-6	2014	Bufalin down regulated the expression levels of nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) during these treatments.	bufalin	0-7	interleukin 1 beta	Homo sapiens	104-121
24719521-6	2014	Bufalin down regulated the expression levels of nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) during these treatments.	bufalin	0-7	interleukin 1 beta	Homo sapiens	123-131
24719521-6	2014	Bufalin down regulated the expression levels of nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) during these treatments.	bufalin	0-7	interleukin 6	Homo sapiens	134-147
24719521-6	2014	Bufalin down regulated the expression levels of nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) during these treatments.	bufalin	0-7	interleukin 6	Homo sapiens	149-153
24719521-6	2014	Bufalin down regulated the expression levels of nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) during these treatments.	bufalin	0-7	tumor necrosis factor	Homo sapiens	160-187
24719521-6	2014	Bufalin down regulated the expression levels of nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) during these treatments.	bufalin	0-7	tumor necrosis factor	Homo sapiens	189-198
24267199-7	2013	RESULTS: Bufalin was found to induce the expression of miR-181a, a small non-coding RNA believed to induce apoptosis by repressing its target gene, BCL-2.	bufalin	9-16	BCL2 apoptosis regulator	Homo sapiens	148-153
24267199-8	2013	In prostate cancer PC-3cell line, bufalin-induced apoptosis can be largely attenuated by a miR-181a inhibitor, which blocked bufalin-induced Bcl-2 reduction and caspase-3 activation.	bufalin	34-41	BCL2 apoptosis regulator	Homo sapiens	141-146
24173654-10	2014	Bufalin significantly arrested the cell cycle at the G0/G1 phase, induced apoptosis through an increase in the Bax/Bcl-xL ratio, inhibited drug efflux pump activity via downregulation of MRP1, and reduced the expression of TS in BEL-7402/5-FU cells.	bufalin	0-7	BCL2 associated X, apoptosis regulator	Homo sapiens	111-114
24173654-10	2014	Bufalin significantly arrested the cell cycle at the G0/G1 phase, induced apoptosis through an increase in the Bax/Bcl-xL ratio, inhibited drug efflux pump activity via downregulation of MRP1, and reduced the expression of TS in BEL-7402/5-FU cells.	bufalin	0-7	BCL2 like 1	Homo sapiens	115-121
24173654-10	2014	Bufalin significantly arrested the cell cycle at the G0/G1 phase, induced apoptosis through an increase in the Bax/Bcl-xL ratio, inhibited drug efflux pump activity via downregulation of MRP1, and reduced the expression of TS in BEL-7402/5-FU cells.	bufalin	0-7	ATP binding cassette subfamily B member 1	Homo sapiens	187-191
24173654-10	2014	Bufalin significantly arrested the cell cycle at the G0/G1 phase, induced apoptosis through an increase in the Bax/Bcl-xL ratio, inhibited drug efflux pump activity via downregulation of MRP1, and reduced the expression of TS in BEL-7402/5-FU cells.	bufalin	0-7	thymidylate synthetase	Homo sapiens	223-225
24267199-8	2013	In prostate cancer PC-3cell line, bufalin-induced apoptosis can be largely attenuated by a miR-181a inhibitor, which blocked bufalin-induced Bcl-2 reduction and caspase-3 activation.	bufalin	125-132	BCL2 apoptosis regulator	Homo sapiens	141-146
24267199-8	2013	In prostate cancer PC-3cell line, bufalin-induced apoptosis can be largely attenuated by a miR-181a inhibitor, which blocked bufalin-induced Bcl-2 reduction and caspase-3 activation.	bufalin	125-132	caspase 3	Homo sapiens	161-170
23799775-4	2013	It was found that bufalin rapidly elicited activation of the ClC-3 Cl(-) channel and subsequently induced apoptosis through inhibition of the PI3K/Akt/mTOR pathway.	bufalin	18-25	chloride voltage-gated channel 3	Homo sapiens	61-66
24026006-1	2013	Bufalin 5beta-hydroxylation was found to be an isoform-specific biotransformation probe substrate for cytochrome P450 3A4 (CYP3A4).	bufalin	0-7	cytochrome P450 family 3 subfamily A member 4	Homo sapiens	102-121
24026006-1	2013	Bufalin 5beta-hydroxylation was found to be an isoform-specific biotransformation probe substrate for cytochrome P450 3A4 (CYP3A4).	bufalin	0-7	cytochrome P450 family 3 subfamily A member 4	Homo sapiens	123-129
23615397-4	2013	Here, we report that bufalin and other cardiac glycosides potently induce mitotic arrest by the downregulation of polo-like kinase 1 (Plk1) expression.	bufalin	21-28	polo like kinase 1	Homo sapiens	114-132
23615397-4	2013	Here, we report that bufalin and other cardiac glycosides potently induce mitotic arrest by the downregulation of polo-like kinase 1 (Plk1) expression.	bufalin	21-28	polo like kinase 1	Homo sapiens	134-138
23799775-4	2013	It was found that bufalin rapidly elicited activation of the ClC-3 Cl(-) channel and subsequently induced apoptosis through inhibition of the PI3K/Akt/mTOR pathway.	bufalin	18-25	AKT serine/threonine kinase 1	Homo sapiens	147-150
23799775-4	2013	It was found that bufalin rapidly elicited activation of the ClC-3 Cl(-) channel and subsequently induced apoptosis through inhibition of the PI3K/Akt/mTOR pathway.	bufalin	18-25	mechanistic target of rapamycin kinase	Homo sapiens	151-155
23677253-0	2013	Bufalin induces G2/M phase arrest and triggers autophagy via the TNF, JNK, BECN-1 and ATG8 pathway in human hepatoma cells.	bufalin	0-7	tumor necrosis factor	Homo sapiens	65-68
23870199-3	2013	In the present study, we investigated the effect of bufalin on the inhibition of an AKT-related signaling pathway, and examined the relationship between regulatory proteins and anti-tumor effects in hepatoma cells.	bufalin	52-59	AKT serine/threonine kinase 1	Homo sapiens	84-87
23870199-8	2013	In addition, treatment with bufalin significantly decreased the levels of pAKT, pGSK3beta, MMP-9, and MMP-2, while increasing the levels of GSK3beta and E-cadherin and suppressing the nuclear translocation of beta-catenin.	bufalin	28-35	matrix metallopeptidase 9	Homo sapiens	91-96
23870199-8	2013	In addition, treatment with bufalin significantly decreased the levels of pAKT, pGSK3beta, MMP-9, and MMP-2, while increasing the levels of GSK3beta and E-cadherin and suppressing the nuclear translocation of beta-catenin.	bufalin	28-35	matrix metallopeptidase 2	Homo sapiens	102-107
23870199-8	2013	In addition, treatment with bufalin significantly decreased the levels of pAKT, pGSK3beta, MMP-9, and MMP-2, while increasing the levels of GSK3beta and E-cadherin and suppressing the nuclear translocation of beta-catenin.	bufalin	28-35	glycogen synthase kinase 3 beta	Homo sapiens	81-89
23870199-8	2013	In addition, treatment with bufalin significantly decreased the levels of pAKT, pGSK3beta, MMP-9, and MMP-2, while increasing the levels of GSK3beta and E-cadherin and suppressing the nuclear translocation of beta-catenin.	bufalin	28-35	cadherin 1	Homo sapiens	153-163
23870199-8	2013	In addition, treatment with bufalin significantly decreased the levels of pAKT, pGSK3beta, MMP-9, and MMP-2, while increasing the levels of GSK3beta and E-cadherin and suppressing the nuclear translocation of beta-catenin.	bufalin	28-35	catenin beta 1	Homo sapiens	209-221
23870199-9	2013	CONCLUSIONS: Bufalin is a potential anti-HCC therapeutic candidate through its inhibition of the AKT/GSK3beta/beta-catenin/E-cadherin signaling pathway.	bufalin	13-20	AKT serine/threonine kinase 1	Homo sapiens	97-100
23870199-9	2013	CONCLUSIONS: Bufalin is a potential anti-HCC therapeutic candidate through its inhibition of the AKT/GSK3beta/beta-catenin/E-cadherin signaling pathway.	bufalin	13-20	glycogen synthase kinase 3 beta	Homo sapiens	101-109
23870199-9	2013	CONCLUSIONS: Bufalin is a potential anti-HCC therapeutic candidate through its inhibition of the AKT/GSK3beta/beta-catenin/E-cadherin signaling pathway.	bufalin	13-20	catenin beta 1	Homo sapiens	110-122
23870199-9	2013	CONCLUSIONS: Bufalin is a potential anti-HCC therapeutic candidate through its inhibition of the AKT/GSK3beta/beta-catenin/E-cadherin signaling pathway.	bufalin	13-20	cadherin 1	Homo sapiens	123-133
23677253-0	2013	Bufalin induces G2/M phase arrest and triggers autophagy via the TNF, JNK, BECN-1 and ATG8 pathway in human hepatoma cells.	bufalin	0-7	mitogen-activated protein kinase 8	Homo sapiens	70-73
23677253-0	2013	Bufalin induces G2/M phase arrest and triggers autophagy via the TNF, JNK, BECN-1 and ATG8 pathway in human hepatoma cells.	bufalin	0-7	beclin 1	Homo sapiens	75-81
23677253-0	2013	Bufalin induces G2/M phase arrest and triggers autophagy via the TNF, JNK, BECN-1 and ATG8 pathway in human hepatoma cells.	bufalin	0-7	GABA type A receptor associated protein like 1	Homo sapiens	86-90
23677253-5	2013	In this study, we aimed to investigate the efficacy and mechanism of bufalin in Huh7, Hep3B and HA22T human hepatoma cells.	bufalin	69-76	MIR7-3 host gene	Homo sapiens	80-84
23677253-10	2013	Bufalin synergized with the JNK pathway to induce autophagy of hepatoma cells and is closely associated with the upregulation of TNF, BECN-1, MAPK and ATG8, together with the downregulation of Bcl-2 and Bid.	bufalin	0-7	mitogen-activated protein kinase 8	Homo sapiens	28-31
23677253-10	2013	Bufalin synergized with the JNK pathway to induce autophagy of hepatoma cells and is closely associated with the upregulation of TNF, BECN-1, MAPK and ATG8, together with the downregulation of Bcl-2 and Bid.	bufalin	0-7	tumor necrosis factor	Homo sapiens	129-132
23677253-10	2013	Bufalin synergized with the JNK pathway to induce autophagy of hepatoma cells and is closely associated with the upregulation of TNF, BECN-1, MAPK and ATG8, together with the downregulation of Bcl-2 and Bid.	bufalin	0-7	beclin 1	Homo sapiens	134-140
23677253-10	2013	Bufalin synergized with the JNK pathway to induce autophagy of hepatoma cells and is closely associated with the upregulation of TNF, BECN-1, MAPK and ATG8, together with the downregulation of Bcl-2 and Bid.	bufalin	0-7	GABA type A receptor associated protein like 1	Homo sapiens	151-155
23677253-10	2013	Bufalin synergized with the JNK pathway to induce autophagy of hepatoma cells and is closely associated with the upregulation of TNF, BECN-1, MAPK and ATG8, together with the downregulation of Bcl-2 and Bid.	bufalin	0-7	BCL2 apoptosis regulator	Homo sapiens	193-198
23677253-10	2013	Bufalin synergized with the JNK pathway to induce autophagy of hepatoma cells and is closely associated with the upregulation of TNF, BECN-1, MAPK and ATG8, together with the downregulation of Bcl-2 and Bid.	bufalin	0-7	BH3 interacting domain death agonist	Homo sapiens	203-206
23344047-9	2013	Bufalin treatment dose-dependently promoted AMPK phosphorylation while AMPK inhibition by compound C significantly attenuated  bufalin-induced autophagy.	bufalin	0-7	protein kinase AMP-activated catalytic subunit alpha 1	Homo sapiens	44-48
23762475-5	2013	We here showed that bufalin could inhibit PARP1 activity in vitro and reduce DNA-damage-induced poly(ADP-ribosyl)ation in MM cells.	bufalin	20-27	poly(ADP-ribose) polymerase 1	Homo sapiens	42-47
23762475-12	2013	Collectively, our data suggest that bufalin is a novel PARP1 inhibitor and a potentially promising therapeutic agent against MM alone or in combination with other drugs.	bufalin	36-43	poly(ADP-ribose) polymerase 1	Homo sapiens	55-60
24325059-8	2013	Results of Western blot showed the protein expressions of p-EGFR, p-Met, p-Akt, and p-mTOR in H1975 cells could be markedly down-regulated by bufalin plus gefitinib.	bufalin	142-149	epidermal growth factor receptor	Homo sapiens	60-64
24325059-8	2013	Results of Western blot showed the protein expressions of p-EGFR, p-Met, p-Akt, and p-mTOR in H1975 cells could be markedly down-regulated by bufalin plus gefitinib.	bufalin	142-149	AKT serine/threonine kinase 1	Homo sapiens	75-78
24325059-8	2013	Results of Western blot showed the protein expressions of p-EGFR, p-Met, p-Akt, and p-mTOR in H1975 cells could be markedly down-regulated by bufalin plus gefitinib.	bufalin	142-149	mechanistic target of rapamycin kinase	Homo sapiens	86-90
23344047-9	2013	Bufalin treatment dose-dependently promoted AMPK phosphorylation while AMPK inhibition by compound C significantly attenuated  bufalin-induced autophagy.	bufalin	127-134	protein kinase AMP-activated catalytic subunit alpha 1	Homo sapiens	71-75
23344047-10	2013	Taken together, we report for the first time that bufalin induces HepG2 cells PCD, especially for autophagy, and the mechanism of action is, at least in part, AMPK-mTOR dependent.	bufalin	50-57	protein kinase AMP-activated catalytic subunit alpha 1	Homo sapiens	159-163
23344047-10	2013	Taken together, we report for the first time that bufalin induces HepG2 cells PCD, especially for autophagy, and the mechanism of action is, at least in part, AMPK-mTOR dependent.	bufalin	50-57	mechanistic target of rapamycin kinase	Homo sapiens	164-168
23533466-0	2013	Bufalin Reverses HGF-Induced Resistance to EGFR-TKIs in EGFR Mutant Lung Cancer Cells via Blockage of Met/PI3k/Akt Pathway and Induction of Apoptosis.	bufalin	0-7	hepatocyte growth factor	Homo sapiens	17-20
23533466-0	2013	Bufalin Reverses HGF-Induced Resistance to EGFR-TKIs in EGFR Mutant Lung Cancer Cells via Blockage of Met/PI3k/Akt Pathway and Induction of Apoptosis.	bufalin	0-7	epidermal growth factor receptor	Homo sapiens	43-47
23533466-0	2013	Bufalin Reverses HGF-Induced Resistance to EGFR-TKIs in EGFR Mutant Lung Cancer Cells via Blockage of Met/PI3k/Akt Pathway and Induction of Apoptosis.	bufalin	0-7	epidermal growth factor receptor	Homo sapiens	56-60
23533466-0	2013	Bufalin Reverses HGF-Induced Resistance to EGFR-TKIs in EGFR Mutant Lung Cancer Cells via Blockage of Met/PI3k/Akt Pathway and Induction of Apoptosis.	bufalin	0-7	AKT serine/threonine kinase 1	Homo sapiens	111-114
23533466-5	2013	The purpose of this study was to determine whether bufalin, a major bioactive component of Venenum Bufonis, could reverse HGF-induced resistance to reversible and irreversible EGFR-TKIs in mutant lung cancer cells PC-9, HCC827, and H1975.	bufalin	51-58	hepatocyte growth factor	Homo sapiens	122-125
23234791-9	2012	However, bufalin treatment enhanced GJIC function, decreased Cx45 mRNA and Cx40 mRNA expression (P&lt;0.01), and reduced [Ca(2+)]i (P&lt;0.01).	bufalin	9-16	gap junction protein, gamma 1	Rattus norvegicus	61-65
23128598-5	2013	Bufalin treatment also repressed             the levels of claudin proteins (claudin-2, -3 and -4) and the major components             of TJs that play key roles in the control and selectivity of paracellular transport.	bufalin	0-7	claudin 2	Homo sapiens	77-97
23128598-8	2013	The inhibition of ERK (PD98059) significantly prevented the bufalin-induced suppression             of T24 cell migration.	bufalin	60-67	mitogen-activated protein kinase 1	Homo sapiens	18-21
23533466-5	2013	The purpose of this study was to determine whether bufalin, a major bioactive component of Venenum Bufonis, could reverse HGF-induced resistance to reversible and irreversible EGFR-TKIs in mutant lung cancer cells PC-9, HCC827, and H1975.	bufalin	51-58	epidermal growth factor receptor	Homo sapiens	176-180
23533466-6	2013	Our studies showed that bufalin could reverse resistance to reversible and irreversible EGFR-TKIs induced by exogenous HGF in EGFR mutant lung cancer cells by inhibiting the Met/PI3K/Akt pathway and inducing death signaling.	bufalin	24-31	epidermal growth factor receptor	Homo sapiens	88-92
23533466-6	2013	Our studies showed that bufalin could reverse resistance to reversible and irreversible EGFR-TKIs induced by exogenous HGF in EGFR mutant lung cancer cells by inhibiting the Met/PI3K/Akt pathway and inducing death signaling.	bufalin	24-31	hepatocyte growth factor	Homo sapiens	119-122
23533466-6	2013	Our studies showed that bufalin could reverse resistance to reversible and irreversible EGFR-TKIs induced by exogenous HGF in EGFR mutant lung cancer cells by inhibiting the Met/PI3K/Akt pathway and inducing death signaling.	bufalin	24-31	epidermal growth factor receptor	Homo sapiens	126-130
23533466-6	2013	Our studies showed that bufalin could reverse resistance to reversible and irreversible EGFR-TKIs induced by exogenous HGF in EGFR mutant lung cancer cells by inhibiting the Met/PI3K/Akt pathway and inducing death signaling.	bufalin	24-31	AKT serine/threonine kinase 1	Homo sapiens	183-186
23286398-1	2012	OBJECTIVE: To investigate the effect of bufalin on nucleus-mitochondria localization of human telomerase reverse transcriptase(hTERT) by exploring its effect on proliferation and apoptosis in human esophageal squamous carcinoma EC9706 cells.	bufalin	40-47	telomerase reverse transcriptase	Homo sapiens	127-132
23286398-11	2012	hTERT is localized in both nucleus and mitochondria,and can be partially translocated from nucleus to mitochondria during the bufalin-induced apoptosis.	bufalin	126-133	telomerase reverse transcriptase	Homo sapiens	0-5
23234791-9	2012	However, bufalin treatment enhanced GJIC function, decreased Cx45 mRNA and Cx40 mRNA expression (P&lt;0.01), and reduced [Ca(2+)]i (P&lt;0.01).	bufalin	9-16	gap junction protein, alpha 5	Rattus norvegicus	75-79
22922805-8	2012	In contrast,             CAL 27 cells overexpressing constitutively active AKT (CAL 27/CA-AKT) were exposed             to bufalin at different concentrations, and cell growth remained unchanged.	bufalin	123-130	AKT serine/threonine kinase 1	Homo sapiens	90-93
22922805-9	2012	Bufalin             exhibited minimal apoptotic effects on CAL 27/CA-AKT cells.	bufalin	0-7	AKT serine/threonine kinase 1	Homo sapiens	69-72
22922805-10	2012	Taken together, bufalin             induced G0/G1 phase arrest and provoked the intrinsic apoptotic pathway via AKT             activation in CAL 27 cells.	bufalin	16-23	AKT serine/threonine kinase 1	Homo sapiens	112-115
22858649-0	2012	Bufalin increases sensitivity to AKT/mTOR-induced autophagic cell death in SK-HEP-1 human hepatocellular carcinoma cells.	bufalin	0-7	AKT serine/threonine kinase 1	Homo sapiens	33-36
23110625-0	2012	Inhibition of Jak-STAT3 pathway enhances bufalin-induced apoptosis in colon cancer SW620 cells.	bufalin	41-48	signal transducer and activator of transcription 3	Homo sapiens	18-23
23110625-1	2012	BACKGROUND: The purpose of the research is to investigate the roles of Jak-STAT3 signaling pathway in bufalin-induced apoptosis in colon cancer SW620 cells.	bufalin	102-109	signal transducer and activator of transcription 3	Homo sapiens	75-80
23110625-10	2012	(4) During the process of bufalin-induced apoptosis in SW620 cells, transient activation of p-stat3 inhibited the activation of stat3, up-regulated Bax expression, down-regulated livin and Bcl-2 expression (P&lt;0.01), and activated caspase-3.	bufalin	26-33	signal transducer and activator of transcription 3	Homo sapiens	94-99
23110625-10	2012	(4) During the process of bufalin-induced apoptosis in SW620 cells, transient activation of p-stat3 inhibited the activation of stat3, up-regulated Bax expression, down-regulated livin and Bcl-2 expression (P&lt;0.01), and activated caspase-3.	bufalin	26-33	signal transducer and activator of transcription 3	Homo sapiens	128-133
23110625-10	2012	(4) During the process of bufalin-induced apoptosis in SW620 cells, transient activation of p-stat3 inhibited the activation of stat3, up-regulated Bax expression, down-regulated livin and Bcl-2 expression (P&lt;0.01), and activated caspase-3.	bufalin	26-33	BCL2 associated X, apoptosis regulator	Homo sapiens	148-151
23110625-10	2012	(4) During the process of bufalin-induced apoptosis in SW620 cells, transient activation of p-stat3 inhibited the activation of stat3, up-regulated Bax expression, down-regulated livin and Bcl-2 expression (P&lt;0.01), and activated caspase-3.	bufalin	26-33	BCL2 apoptosis regulator	Homo sapiens	189-194
23110625-10	2012	(4) During the process of bufalin-induced apoptosis in SW620 cells, transient activation of p-stat3 inhibited the activation of stat3, up-regulated Bax expression, down-regulated livin and Bcl-2 expression (P&lt;0.01), and activated caspase-3.	bufalin	26-33	caspase 3	Homo sapiens	233-242
23110625-11	2012	Inhibition of Jak-stat3 signaling pathway by pre-treatment with AG490 significantly enhanced the bufalin-induced apoptosis (P&lt;0.01), further up-regulated Bax protein expression, down-regulated livin and Bcl-2 protein expression and enhanced caspase-3 activation.	bufalin	97-104	signal transducer and activator of transcription 3	Homo sapiens	18-23
23110625-11	2012	Inhibition of Jak-stat3 signaling pathway by pre-treatment with AG490 significantly enhanced the bufalin-induced apoptosis (P&lt;0.01), further up-regulated Bax protein expression, down-regulated livin and Bcl-2 protein expression and enhanced caspase-3 activation.	bufalin	97-104	BCL2 apoptosis regulator	Homo sapiens	206-211
23110625-11	2012	Inhibition of Jak-stat3 signaling pathway by pre-treatment with AG490 significantly enhanced the bufalin-induced apoptosis (P&lt;0.01), further up-regulated Bax protein expression, down-regulated livin and Bcl-2 protein expression and enhanced caspase-3 activation.	bufalin	97-104	caspase 3	Homo sapiens	244-253
23110625-13	2012	Activation of caspase-3, up-regulation of Bax, down-regulation of livin and Bcl-2, as well as inhibition of Jak-stat3 signaling pathway might be the important mechanisms for the bufalin-induced apoptosis.	bufalin	178-185	caspase 3	Homo sapiens	14-23
23110625-13	2012	Activation of caspase-3, up-regulation of Bax, down-regulation of livin and Bcl-2, as well as inhibition of Jak-stat3 signaling pathway might be the important mechanisms for the bufalin-induced apoptosis.	bufalin	178-185	BCL2 associated X, apoptosis regulator	Homo sapiens	42-45
23110625-13	2012	Activation of caspase-3, up-regulation of Bax, down-regulation of livin and Bcl-2, as well as inhibition of Jak-stat3 signaling pathway might be the important mechanisms for the bufalin-induced apoptosis.	bufalin	178-185	baculoviral IAP repeat containing 7	Homo sapiens	66-71
23110625-13	2012	Activation of caspase-3, up-regulation of Bax, down-regulation of livin and Bcl-2, as well as inhibition of Jak-stat3 signaling pathway might be the important mechanisms for the bufalin-induced apoptosis.	bufalin	178-185	BCL2 apoptosis regulator	Homo sapiens	76-81
23110625-13	2012	Activation of caspase-3, up-regulation of Bax, down-regulation of livin and Bcl-2, as well as inhibition of Jak-stat3 signaling pathway might be the important mechanisms for the bufalin-induced apoptosis.	bufalin	178-185	signal transducer and activator of transcription 3	Homo sapiens	112-117
22858649-0	2012	Bufalin increases sensitivity to AKT/mTOR-induced autophagic cell death in SK-HEP-1 human hepatocellular carcinoma cells.	bufalin	0-7	mechanistic target of rapamycin kinase	Homo sapiens	37-41
22858649-0	2012	Bufalin increases sensitivity to AKT/mTOR-induced autophagic cell death in SK-HEP-1 human hepatocellular carcinoma cells.	bufalin	0-7	DNL-type zinc finger	Homo sapiens	78-83
22858649-2	2012	In the present study, we investigated the pharmacological mechanisms of cell cycle arrest and autophagic cell death induced by bufalin in SK-HEP-1 human hepatocellular carcinoma cells in vitro.	bufalin	127-134	DNL-type zinc finger	Homo sapiens	141-146
22858649-4	2012	In addition, bufalin induced G2/M phase arrest by reducing CDK1 activity.	bufalin	13-20	cyclin dependent kinase 1	Homo sapiens	59-63
22858649-5	2012	Bufalin triggered DNA fragmentation and apoptotic cell death in SK-HEP-1 cells by DNA gel electrophoresis, TUNEL and caspase-3 activity assay, while bufalin induced autophagic cell death by double-membrane vacuoles (transmission electron microscopy, TEM), acidic vesicular organelles (acridine orange staining) and cleavage of microtubule-associated protein 1 light chain 3 (LC3).	bufalin	0-7	DNL-type zinc finger	Homo sapiens	67-72
22858649-5	2012	Bufalin triggered DNA fragmentation and apoptotic cell death in SK-HEP-1 cells by DNA gel electrophoresis, TUNEL and caspase-3 activity assay, while bufalin induced autophagic cell death by double-membrane vacuoles (transmission electron microscopy, TEM), acidic vesicular organelles (acridine orange staining) and cleavage of microtubule-associated protein 1 light chain 3 (LC3).	bufalin	0-7	caspase 3	Homo sapiens	117-126
22858649-7	2012	In contrast, protein expression levels of the Chk1, Wee1, LC3-II, Beclin-1, Atg 5, Atg 7 and Atg 12 were upregulated in SK-HEP-1 cells after bufalin treatment.	bufalin	141-148	checkpoint kinase 1	Homo sapiens	46-50
22858649-7	2012	In contrast, protein expression levels of the Chk1, Wee1, LC3-II, Beclin-1, Atg 5, Atg 7 and Atg 12 were upregulated in SK-HEP-1 cells after bufalin treatment.	bufalin	141-148	WEE1 G2 checkpoint kinase	Homo sapiens	52-56
22858649-7	2012	In contrast, protein expression levels of the Chk1, Wee1, LC3-II, Beclin-1, Atg 5, Atg 7 and Atg 12 were upregulated in SK-HEP-1 cells after bufalin treatment.	bufalin	141-148	beclin 1	Homo sapiens	66-74
22922805-0	2012	AKT serine/threonine protein kinase modulates bufalin-triggered intrinsic             pathway of apoptosis in CAL 27 human oral cancer cells.	bufalin	46-53	AKT serine/threonine kinase 1	Homo sapiens	0-3
22858649-7	2012	In contrast, protein expression levels of the Chk1, Wee1, LC3-II, Beclin-1, Atg 5, Atg 7 and Atg 12 were upregulated in SK-HEP-1 cells after bufalin treatment.	bufalin	141-148	autophagy related 5	Homo sapiens	76-81
22922805-6	2012	An increase in the expression of the active form             of caspases was found in bufalin-treated cells, and the caspase activities were             also elevated.	bufalin	86-93	caspase 9	Homo sapiens	64-72
22858649-7	2012	In contrast, protein expression levels of the Chk1, Wee1, LC3-II, Beclin-1, Atg 5, Atg 7 and Atg 12 were upregulated in SK-HEP-1 cells after bufalin treatment.	bufalin	141-148	autophagy related 7	Homo sapiens	83-88
22922805-6	2012	An increase in the expression of the active form             of caspases was found in bufalin-treated cells, and the caspase activities were             also elevated.	bufalin	86-93	caspase 9	Homo sapiens	64-71
22922805-7	2012	Bufalin-triggered apoptosis was blocked by specific inhibitors             of caspase-9 (z-LEHD-fmk) and caspase-3 (z-DEVD-fmk), respectively.	bufalin	0-7	caspase 9	Homo sapiens	78-87
22922805-7	2012	Bufalin-triggered apoptosis was blocked by specific inhibitors             of caspase-9 (z-LEHD-fmk) and caspase-3 (z-DEVD-fmk), respectively.	bufalin	0-7	caspase 3	Homo sapiens	105-114
22858649-7	2012	In contrast, protein expression levels of the Chk1, Wee1, LC3-II, Beclin-1, Atg 5, Atg 7 and Atg 12 were upregulated in SK-HEP-1 cells after bufalin treatment.	bufalin	141-148	DNL-type zinc finger	Homo sapiens	123-128
22922805-8	2012	In contrast,             CAL 27 cells overexpressing constitutively active AKT (CAL 27/CA-AKT) were exposed             to bufalin at different concentrations, and cell growth remained unchanged.	bufalin	123-130	AKT serine/threonine kinase 1	Homo sapiens	75-78
23019887-10	2012	Western blotting showed consistent results in heat shock protein 27, vimentin and HNRPK between expression after treatment with bufalin and two-dimensional electrophoresis.	bufalin	128-135	heterogeneous nuclear ribonucleoprotein K	Homo sapiens	82-87
23205102-5	2012	The activity of apoptosis signal-regulating kinase 1 (ASK1)/JNK was upregulated in gemcitabine-induced apoptosis when combined with bufalin.	bufalin	132-139	mitogen-activated protein kinase kinase kinase 5	Mus musculus	16-52
23205102-5	2012	The activity of apoptosis signal-regulating kinase 1 (ASK1)/JNK was upregulated in gemcitabine-induced apoptosis when combined with bufalin.	bufalin	132-139	mitogen-activated protein kinase kinase kinase 5	Mus musculus	54-58
23205102-5	2012	The activity of apoptosis signal-regulating kinase 1 (ASK1)/JNK was upregulated in gemcitabine-induced apoptosis when combined with bufalin.	bufalin	132-139	mitogen-activated protein kinase 8	Mus musculus	60-63
23205102-7	2012	These results suggest that bufalin may be a potential chemotherapeutic agent for pancreatic cancer, which could enhance the antitumor efficacy of gemcitabine when used in combination, possibly through the activation of ASK1/JNK.	bufalin	27-34	mitogen-activated protein kinase kinase kinase 5	Mus musculus	219-223
23205102-7	2012	These results suggest that bufalin may be a potential chemotherapeutic agent for pancreatic cancer, which could enhance the antitumor efficacy of gemcitabine when used in combination, possibly through the activation of ASK1/JNK.	bufalin	27-34	mitogen-activated protein kinase 8	Mus musculus	224-227
22447040-7	2012	Transfection studies were used to determine the involvement of DR4, DR5 and Cbl-b in the synergistic effect of bufalin and TRAIL.	bufalin	111-118	major histocompatibility complex, class II, DR beta 4	Homo sapiens	63-66
22447040-7	2012	Transfection studies were used to determine the involvement of DR4, DR5 and Cbl-b in the synergistic effect of bufalin and TRAIL.	bufalin	111-118	TNF receptor superfamily member 10b	Homo sapiens	68-71
22447040-7	2012	Transfection studies were used to determine the involvement of DR4, DR5 and Cbl-b in the synergistic effect of bufalin and TRAIL.	bufalin	111-118	Cbl proto-oncogene B	Homo sapiens	76-81
22447040-9	2012	Both cell lines were dramatically sensitized to TRAIL-induced apoptosis by bufalin.	bufalin	75-82	TNF superfamily member 10	Homo sapiens	48-53
22447040-11	2012	Blocking the up-regulation of DR4 and DR5 by siRNA rendered cells less sensitive to apoptosis induced by the combination of bufalin and TRAIL.	bufalin	124-131	major histocompatibility complex, class II, DR beta 4	Homo sapiens	30-33
22447040-11	2012	Blocking the up-regulation of DR4 and DR5 by siRNA rendered cells less sensitive to apoptosis induced by the combination of bufalin and TRAIL.	bufalin	124-131	TNF receptor superfamily member 10b	Homo sapiens	38-41
22447040-13	2012	Moreover, down-regulation of Cbl-b by shRNA led to stronger activation of ERK, JNK and p38 MAPK, more up-regulation of DR4 and DR5, and a stronger synergistic effect of bufalin and TRAIL.	bufalin	169-176	Cbl proto-oncogene B	Homo sapiens	29-34
22447040-15	2012	Bufalin-induced down-regulation of Cbl-b contributed to the up-regulation of DR4 and DR5, which might be partially mediated by the activation of ERK, JNK and p38 MAPK.	bufalin	0-7	Cbl proto-oncogene B	Homo sapiens	35-40
22447040-15	2012	Bufalin-induced down-regulation of Cbl-b contributed to the up-regulation of DR4 and DR5, which might be partially mediated by the activation of ERK, JNK and p38 MAPK.	bufalin	0-7	major histocompatibility complex, class II, DR beta 4	Homo sapiens	77-80
22447040-15	2012	Bufalin-induced down-regulation of Cbl-b contributed to the up-regulation of DR4 and DR5, which might be partially mediated by the activation of ERK, JNK and p38 MAPK.	bufalin	0-7	TNF receptor superfamily member 10b	Homo sapiens	85-88
22447040-15	2012	Bufalin-induced down-regulation of Cbl-b contributed to the up-regulation of DR4 and DR5, which might be partially mediated by the activation of ERK, JNK and p38 MAPK.	bufalin	0-7	mitogen-activated protein kinase 8	Homo sapiens	150-153
22447040-15	2012	Bufalin-induced down-regulation of Cbl-b contributed to the up-regulation of DR4 and DR5, which might be partially mediated by the activation of ERK, JNK and p38 MAPK.	bufalin	0-7	mitogen-activated protein kinase 14	Homo sapiens	158-161
21617941-0	2012	Bufalin enhances the anti-proliferative effect of sorafenib on human hepatocellular carcinoma cells through downregulation of ERK.	bufalin	0-7	EPH receptor B2	Homo sapiens	126-129
22207085-7	2012	Bufalin alleviated the removal of podocyte foot processes and attenuated the changes in nephrin, podocin and integrin-linked kinase (ILK) stainings in the glomerulus of the ADR rats.	bufalin	0-7	NPHS1 adhesion molecule, nephrin	Rattus norvegicus	88-95
22207085-7	2012	Bufalin alleviated the removal of podocyte foot processes and attenuated the changes in nephrin, podocin and integrin-linked kinase (ILK) stainings in the glomerulus of the ADR rats.	bufalin	0-7	NPHS2 stomatin family member, podocin	Rattus norvegicus	97-104
22207085-7	2012	Bufalin alleviated the removal of podocyte foot processes and attenuated the changes in nephrin, podocin and integrin-linked kinase (ILK) stainings in the glomerulus of the ADR rats.	bufalin	0-7	integrin-linked kinase	Rattus norvegicus	109-131
22207085-7	2012	Bufalin alleviated the removal of podocyte foot processes and attenuated the changes in nephrin, podocin and integrin-linked kinase (ILK) stainings in the glomerulus of the ADR rats.	bufalin	0-7	integrin-linked kinase	Rattus norvegicus	133-136
22207085-8	2012	Bufalin notably decreased the expression of nephrin and ILK but inhibited the down-regulation of podocin in protein levels on the renal cortex of the ADR rats.	bufalin	0-7	NPHS1 adhesion molecule, nephrin	Rattus norvegicus	44-51
22207085-8	2012	Bufalin notably decreased the expression of nephrin and ILK but inhibited the down-regulation of podocin in protein levels on the renal cortex of the ADR rats.	bufalin	0-7	integrin-linked kinase	Rattus norvegicus	56-59
22207085-8	2012	Bufalin notably decreased the expression of nephrin and ILK but inhibited the down-regulation of podocin in protein levels on the renal cortex of the ADR rats.	bufalin	0-7	NPHS2 stomatin family member, podocin	Rattus norvegicus	97-104
22207085-9	2012	Additionally, bufalin inhibited the up-regulation of podocin and ILK in mRNA levels but did not affect nephrin mRNA levels.	bufalin	14-21	NPHS2 stomatin family member, podocin	Rattus norvegicus	53-60
22207085-9	2012	Additionally, bufalin inhibited the up-regulation of podocin and ILK in mRNA levels but did not affect nephrin mRNA levels.	bufalin	14-21	integrin-linked kinase	Rattus norvegicus	65-68
22285700-0	2012	Bufalin induces G0/G1 phase arrest through inhibiting the levels of cyclin D, cyclin E, CDK2 and CDK4, and triggers apoptosis via mitochondrial signaling pathway in T24 human bladder cancer cells.	bufalin	0-7	cyclin dependent kinase 2	Homo sapiens	88-92
22285700-0	2012	Bufalin induces G0/G1 phase arrest through inhibiting the levels of cyclin D, cyclin E, CDK2 and CDK4, and triggers apoptosis via mitochondrial signaling pathway in T24 human bladder cancer cells.	bufalin	0-7	cyclin dependent kinase 4	Homo sapiens	97-101
22285700-9	2012	The down-regulation of the protein levels for cyclin D, CDK4, cyclin E, CDK2, phospho-Rb, phospho-AKT and Bcl-2 with the simultaneous up-regulation of the cytochrome c, Apaf-1, AIF, caspase-3, -7 and -9 and Bax protein expressions and caspase activities were observed in T24 cells after bufalin treatment.	bufalin	287-294	cytochrome c, somatic	Homo sapiens	155-167
22285700-10	2012	Based on our results, bufalin induces apoptotic cell death in T24 cells through suppressing AKT activity and anti-apoptotic Bcl-2 protein as well as inducing pro-apoptotic Bax protein.	bufalin	22-29	AKT serine/threonine kinase 1	Homo sapiens	92-95
22285700-10	2012	Based on our results, bufalin induces apoptotic cell death in T24 cells through suppressing AKT activity and anti-apoptotic Bcl-2 protein as well as inducing pro-apoptotic Bax protein.	bufalin	22-29	BCL2 apoptosis regulator	Homo sapiens	124-129
22285700-10	2012	Based on our results, bufalin induces apoptotic cell death in T24 cells through suppressing AKT activity and anti-apoptotic Bcl-2 protein as well as inducing pro-apoptotic Bax protein.	bufalin	22-29	BCL2 associated X, apoptosis regulator	Homo sapiens	172-175
22285700-11	2012	The levels of caspase-3, -7 and -9 are also mediated apoptosis in bufalin-treated T24 cells.	bufalin	66-73	caspase 3	Homo sapiens	14-34
22237798-9	2012	Furthermore, the phosphorylated (activated) ERK1/2 expression was increased after bufalin perfusion and this effect was totally prevented after PP2 pretreatment.	bufalin	82-89	mitogen activated protein kinase 3	Rattus norvegicus	44-50
21913902-0	2012	Inhibitory effect of bufalin and cinobufagin on steroidogenesis via the activation of ERK in human adrenocortical cells.	bufalin	21-28	mitogen-activated protein kinase 1	Homo sapiens	86-89
21913902-6	2012	KEY RESULTS: Bufalin and cinobufagin markedly inhibited basal, Ang II-, forskolin- or 8-Br-cAMP-stimulated aldosterone and cortisol secretion, and the conversions of corticosterone to aldosterone and deoxycortisol to cortisol.	bufalin	13-20	angiotensinogen	Homo sapiens	63-69
21913902-7	2012	Bufalin and cinobufagin also inhibited StAR protein expression and SF-1 binding to StAR gene promoter.	bufalin	0-7	steroidogenic acute regulatory protein	Homo sapiens	39-43
21913902-7	2012	Bufalin and cinobufagin also inhibited StAR protein expression and SF-1 binding to StAR gene promoter.	bufalin	0-7	splicing factor 1	Homo sapiens	67-71
21913902-7	2012	Bufalin and cinobufagin also inhibited StAR protein expression and SF-1 binding to StAR gene promoter.	bufalin	0-7	steroidogenic acute regulatory protein	Homo sapiens	83-87
21913902-9	2012	Furthermore, U0126 reversed the inhibitory effects of bufalin and cinobufagin on StAR protein expression and the binding of SF-1 to StAR gene promoter.	bufalin	54-61	steroidogenic acute regulatory protein	Homo sapiens	81-85
21913902-9	2012	Furthermore, U0126 reversed the inhibitory effects of bufalin and cinobufagin on StAR protein expression and the binding of SF-1 to StAR gene promoter.	bufalin	54-61	splicing factor 1	Homo sapiens	124-128
21913902-9	2012	Furthermore, U0126 reversed the inhibitory effects of bufalin and cinobufagin on StAR protein expression and the binding of SF-1 to StAR gene promoter.	bufalin	54-61	steroidogenic acute regulatory protein	Homo sapiens	132-136
21913902-11	2012	CONCLUSIONS AND IMPLICATIONS: The inhibitory effects of bufalin and cinobufagin on steroidogenesis of aldosterone and cortisol were associated with inhibition of aldosterone synthase and 11beta-hydroxylase, as well as the suppression of StAR protein expression and SF-1 binding to StAR promoter via the phosphorylation of ERK1/2 in H295 cells.	bufalin	56-63	steroidogenic acute regulatory protein	Homo sapiens	237-241
21913902-11	2012	CONCLUSIONS AND IMPLICATIONS: The inhibitory effects of bufalin and cinobufagin on steroidogenesis of aldosterone and cortisol were associated with inhibition of aldosterone synthase and 11beta-hydroxylase, as well as the suppression of StAR protein expression and SF-1 binding to StAR promoter via the phosphorylation of ERK1/2 in H295 cells.	bufalin	56-63	splicing factor 1	Homo sapiens	265-269
21913902-11	2012	CONCLUSIONS AND IMPLICATIONS: The inhibitory effects of bufalin and cinobufagin on steroidogenesis of aldosterone and cortisol were associated with inhibition of aldosterone synthase and 11beta-hydroxylase, as well as the suppression of StAR protein expression and SF-1 binding to StAR promoter via the phosphorylation of ERK1/2 in H295 cells.	bufalin	56-63	steroidogenic acute regulatory protein	Homo sapiens	281-285
21913902-11	2012	CONCLUSIONS AND IMPLICATIONS: The inhibitory effects of bufalin and cinobufagin on steroidogenesis of aldosterone and cortisol were associated with inhibition of aldosterone synthase and 11beta-hydroxylase, as well as the suppression of StAR protein expression and SF-1 binding to StAR promoter via the phosphorylation of ERK1/2 in H295 cells.	bufalin	56-63	mitogen-activated protein kinase 3	Homo sapiens	322-328
21617941-3	2012	Exposure of both PLC/PRF/5 and Hep G-2 cells to this combination of sorafenib (6.25 muM) and bufalin (50 nM) resulted in noticeable increases in apoptotic cell death, as evidenced by the disruption of mitochondria, compared to treatment with either agent alone.	bufalin	93-100	heparan sulfate proteoglycan 2	Homo sapiens	17-20
22178124-8	2012	It was found that bufalin, cinobufagin, and resibufogenin were novel CYP3A4 inducers.	bufalin	18-25	cytochrome P450 family 3 subfamily A member 4	Homo sapiens	69-75
22178124-9	2012	Among them, bufalin and cinobufagin significantly promoted the CYP3A4 enzyme activity, mRNA and protein levels, with the maximal induction challenging or exceeding that of the induction by rifampicin, indicating that they might play a critical role in CYP3A4 enzyme-inducing effects of SBP.	bufalin	12-19	cytochrome P450 family 3 subfamily A member 4	Homo sapiens	63-69
22178124-9	2012	Among them, bufalin and cinobufagin significantly promoted the CYP3A4 enzyme activity, mRNA and protein levels, with the maximal induction challenging or exceeding that of the induction by rifampicin, indicating that they might play a critical role in CYP3A4 enzyme-inducing effects of SBP.	bufalin	12-19	cytochrome P450 family 3 subfamily A member 4	Homo sapiens	252-258
22890165-6	2012	Moreover, bufalin synergized with PD98059 to inhibit the proliferation and induce the apoptosis of NB4 cells, which was associated with the downregulation of survivin expression and the upregulation of caspase-3 activation.	bufalin	10-17	caspase 3	Homo sapiens	202-211
23317181-7	2012	Bufalin, bufotalin and gamabufotalin, key bufadienolides, significantly sensitize human breast cancer cells with differing ER-alpha status to apoptosis induction by the TNF-related apoptosis-inducing ligand (TRAIL).	bufalin	0-7	estrogen receptor 1	Homo sapiens	123-131
23317181-7	2012	Bufalin, bufotalin and gamabufotalin, key bufadienolides, significantly sensitize human breast cancer cells with differing ER-alpha status to apoptosis induction by the TNF-related apoptosis-inducing ligand (TRAIL).	bufalin	0-7	TNF superfamily member 10	Homo sapiens	169-206
23317181-7	2012	Bufalin, bufotalin and gamabufotalin, key bufadienolides, significantly sensitize human breast cancer cells with differing ER-alpha status to apoptosis induction by the TNF-related apoptosis-inducing ligand (TRAIL).	bufalin	0-7	TNF superfamily member 10	Homo sapiens	208-213
21617941-4	2012	Although both sorafenib (6.25 muM) and bufalin (250 nM) alone inhibited the phosphorylation of ERK, the reduction in pERK was more pronounced in the cells treated with a combination of bufalin (50 nM) and sorafenib (250 nM).	bufalin	39-46	EPH receptor B2	Homo sapiens	95-98
21617941-4	2012	Although both sorafenib (6.25 muM) and bufalin (250 nM) alone inhibited the phosphorylation of ERK, the reduction in pERK was more pronounced in the cells treated with a combination of bufalin (50 nM) and sorafenib (250 nM).	bufalin	39-46	eukaryotic translation initiation factor 2 alpha kinase 3	Homo sapiens	117-121
21617941-4	2012	Although both sorafenib (6.25 muM) and bufalin (250 nM) alone inhibited the phosphorylation of ERK, the reduction in pERK was more pronounced in the cells treated with a combination of bufalin (50 nM) and sorafenib (250 nM).	bufalin	185-192	EPH receptor B2	Homo sapiens	95-98
21617941-4	2012	Although both sorafenib (6.25 muM) and bufalin (250 nM) alone inhibited the phosphorylation of ERK, the reduction in pERK was more pronounced in the cells treated with a combination of bufalin (50 nM) and sorafenib (250 nM).	bufalin	185-192	eukaryotic translation initiation factor 2 alpha kinase 3	Homo sapiens	117-121
21617941-5	2012	Furthermore, the inhibitory effect of bufalin on pERK was blocked by the PI3kinase inhibitor LY294002, suggesting that the reduction in pERK induced by bufalin might be mediated by AKT in these two HCC cell lines.	bufalin	38-45	eukaryotic translation initiation factor 2 alpha kinase 3	Homo sapiens	49-53
21617941-5	2012	Furthermore, the inhibitory effect of bufalin on pERK was blocked by the PI3kinase inhibitor LY294002, suggesting that the reduction in pERK induced by bufalin might be mediated by AKT in these two HCC cell lines.	bufalin	38-45	eukaryotic translation initiation factor 2 alpha kinase 3	Homo sapiens	136-140
21617941-5	2012	Furthermore, the inhibitory effect of bufalin on pERK was blocked by the PI3kinase inhibitor LY294002, suggesting that the reduction in pERK induced by bufalin might be mediated by AKT in these two HCC cell lines.	bufalin	38-45	AKT serine/threonine kinase 1	Homo sapiens	181-184
21617941-5	2012	Furthermore, the inhibitory effect of bufalin on pERK was blocked by the PI3kinase inhibitor LY294002, suggesting that the reduction in pERK induced by bufalin might be mediated by AKT in these two HCC cell lines.	bufalin	152-159	eukaryotic translation initiation factor 2 alpha kinase 3	Homo sapiens	49-53
21617941-5	2012	Furthermore, the inhibitory effect of bufalin on pERK was blocked by the PI3kinase inhibitor LY294002, suggesting that the reduction in pERK induced by bufalin might be mediated by AKT in these two HCC cell lines.	bufalin	152-159	eukaryotic translation initiation factor 2 alpha kinase 3	Homo sapiens	136-140
21617941-5	2012	Furthermore, the inhibitory effect of bufalin on pERK was blocked by the PI3kinase inhibitor LY294002, suggesting that the reduction in pERK induced by bufalin might be mediated by AKT in these two HCC cell lines.	bufalin	152-159	AKT serine/threonine kinase 1	Homo sapiens	181-184
21617941-6	2012	Taken together, the results of our study suggest that bufalin enhances the anti-cancer effects of sorafenib on PLC/PRF/5 and Hep G-2 by contributing to the downregulation of ERK.	bufalin	54-61	heparan sulfate proteoglycan 2	Homo sapiens	111-114
21617941-6	2012	Taken together, the results of our study suggest that bufalin enhances the anti-cancer effects of sorafenib on PLC/PRF/5 and Hep G-2 by contributing to the downregulation of ERK.	bufalin	54-61	EPH receptor B2	Homo sapiens	174-177
21901250-6	2012	Apoptosis induction of T24 cells by bufalin showed correlation with proteolytic             activation of caspase-3, -8 and -9, and concomitant degradation of poly (ADP-ribose)             polymerases, and collapse of the mitochondria membrane potential.	bufalin	36-43	caspase 3	Homo sapiens	106-126
22408435-0	2012	Bufalin induces lung cancer cell apoptosis via the inhibition of PI3K/Akt pathway.	bufalin	0-7	AKT serine/threonine kinase 1	Homo sapiens	70-73
22408435-5	2012	In addition, A549 cells were treated by Akt inhibitor LY294002 in combination with bufalin and the activation of Akt and Caspase-3 as well as the expression levels of Bax, Bcl-2 and livin were examined by Western blot analysis.	bufalin	83-90	AKT serine/threonine kinase 1	Homo sapiens	40-43
22408435-7	2012	Mechanistically, we found that bufalin inhibited the activation of Akt.	bufalin	31-38	AKT serine/threonine kinase 1	Homo sapiens	67-70
22408435-8	2012	Moreover, bufalin synergized with Akt inhibitor to induce the apoptosis of A549 cells and this was associated with the upregulation of Bax expression, the downregulation of Bcl-2 and livin expression, and the activation of Caspase-3.	bufalin	10-17	AKT serine/threonine kinase 1	Homo sapiens	34-37
22408435-8	2012	Moreover, bufalin synergized with Akt inhibitor to induce the apoptosis of A549 cells and this was associated with the upregulation of Bax expression, the downregulation of Bcl-2 and livin expression, and the activation of Caspase-3.	bufalin	10-17	BCL2 associated X, apoptosis regulator	Homo sapiens	135-138
22408435-8	2012	Moreover, bufalin synergized with Akt inhibitor to induce the apoptosis of A549 cells and this was associated with the upregulation of Bax expression, the downregulation of Bcl-2 and livin expression, and the activation of Caspase-3.	bufalin	10-17	BCL2 apoptosis regulator	Homo sapiens	173-178
22408435-8	2012	Moreover, bufalin synergized with Akt inhibitor to induce the apoptosis of A549 cells and this was associated with the upregulation of Bax expression, the downregulation of Bcl-2 and livin expression, and the activation of Caspase-3.	bufalin	10-17	baculoviral IAP repeat containing 7	Homo sapiens	183-188
22408435-8	2012	Moreover, bufalin synergized with Akt inhibitor to induce the apoptosis of A549 cells and this was associated with the upregulation of Bax expression, the downregulation of Bcl-2 and livin expression, and the activation of Caspase-3.	bufalin	10-17	caspase 3	Homo sapiens	223-232
22408435-9	2012	In conclusion, our findings demonstrate that bufalin induces lung cancer cell apoptosis via the inhibition of PI3K/Akt pathway and suggest that bufalin is a potential regimen for combined chemotherapy to overcome the resistance of lung cancer cells to chemotherapeutics induced apoptosis.	bufalin	45-52	AKT serine/threonine kinase 1	Homo sapiens	115-118
21901250-7	2012	In addition,             bufalin treatment resulted in an increase of the Bax/Bcl-2 (or Bcl-xL) ratio and             caused down-regulation of inhibitor of apoptosis protein (IAP) family members.	bufalin	25-32	BCL2 associated X, apoptosis regulator	Homo sapiens	74-77
21901250-7	2012	In addition,             bufalin treatment resulted in an increase of the Bax/Bcl-2 (or Bcl-xL) ratio and             caused down-regulation of inhibitor of apoptosis protein (IAP) family members.	bufalin	25-32	BCL2 apoptosis regulator	Homo sapiens	78-83
21901250-7	2012	In addition,             bufalin treatment resulted in an increase of the Bax/Bcl-2 (or Bcl-xL) ratio and             caused down-regulation of inhibitor of apoptosis protein (IAP) family members.	bufalin	25-32	BCL2 like 1	Homo sapiens	88-94
21901250-7	2012	In addition,             bufalin treatment resulted in an increase of the Bax/Bcl-2 (or Bcl-xL) ratio and             caused down-regulation of inhibitor of apoptosis protein (IAP) family members.	bufalin	25-32	CD47 molecule	Homo sapiens	144-174
21901250-7	2012	In addition,             bufalin treatment resulted in an increase of the Bax/Bcl-2 (or Bcl-xL) ratio and             caused down-regulation of inhibitor of apoptosis protein (IAP) family members.	bufalin	25-32	CD47 molecule	Homo sapiens	176-179
23091618-9	2012	Hsp27 over-expression protected against bufalin-induced apoptosis, reversed the dephosphorylation of Akt and preserved the level of nuclear NF-kappaB p65 and co-immunoprecipitated Hsp27/cytochrome c.	bufalin	40-47	heat shock protein family B (small) member 1	Homo sapiens	0-5
23091618-9	2012	Hsp27 over-expression protected against bufalin-induced apoptosis, reversed the dephosphorylation of Akt and preserved the level of nuclear NF-kappaB p65 and co-immunoprecipitated Hsp27/cytochrome c.	bufalin	40-47	cytochrome c, somatic	Homo sapiens	186-198
23091618-10	2012	Moreover, bufalin inhibited MTX-resistant osteosarcoma xenograft growth, and a down-regulation of Hsp27 in vivo was observed.	bufalin	10-17	heat shock protein family B (small) member 1	Homo sapiens	98-103
23091618-12	2012	The down-regulation of Hsp27 played a critical role in bufalin-induced apoptosis in osteosarcoma cells.	bufalin	55-62	heat shock protein family B (small) member 1	Homo sapiens	23-28
21719999-6	2011	Furthermore, bufalin not only inhibited upregulation of cyclin D1 and CDK4, but also downregulation of p21 in both mRNA and protein levels.	bufalin	13-20	cyclin D1	Rattus norvegicus	56-65
21288283-7	2011	In addition, caspase activation during bufalin- and cinobufagin-induced apoptosis was further confirmed by caspase-3 inhibitor Z-DEVD-FMK, caspase-8 inhibitor Z-IETD-FMK, caspase-9 inhibitor Z-LEHD-FMK and caspase-10 inhibitor Z-AEVD-FMK.	bufalin	39-46	caspase 3	Homo sapiens	107-116
21288283-7	2011	In addition, caspase activation during bufalin- and cinobufagin-induced apoptosis was further confirmed by caspase-3 inhibitor Z-DEVD-FMK, caspase-8 inhibitor Z-IETD-FMK, caspase-9 inhibitor Z-LEHD-FMK and caspase-10 inhibitor Z-AEVD-FMK.	bufalin	39-46	caspase 8	Homo sapiens	139-148
21288283-7	2011	In addition, caspase activation during bufalin- and cinobufagin-induced apoptosis was further confirmed by caspase-3 inhibitor Z-DEVD-FMK, caspase-8 inhibitor Z-IETD-FMK, caspase-9 inhibitor Z-LEHD-FMK and caspase-10 inhibitor Z-AEVD-FMK.	bufalin	39-46	caspase 9	Homo sapiens	171-180
21288283-7	2011	In addition, caspase activation during bufalin- and cinobufagin-induced apoptosis was further confirmed by caspase-3 inhibitor Z-DEVD-FMK, caspase-8 inhibitor Z-IETD-FMK, caspase-9 inhibitor Z-LEHD-FMK and caspase-10 inhibitor Z-AEVD-FMK.	bufalin	39-46	caspase 10	Homo sapiens	206-216
21288283-8	2011	The results showed that bufalin- and cinobufagin-induced apoptosis was blocked by these inhibitors and particularly by caspase-10 inhibitor.	bufalin	24-31	caspase 10	Homo sapiens	119-129
21259053-4	2011	In the current study, we found that bufalin, bufotalin and gamabufotalin, key members of bufadienolides isolated from a traditional Chinese medicine ChanSu, significantly potentiated human breast cancer cells with different status of ER-alpha to apoptosis induction of TRAIL, as evidenced by enhanced Annexin V/FITC positive cells (apoptotic cells), cytoplasmic histone-associated-DNA-fragments, membrane permeability transition (MPT), caspases activation and PARP cleavage.	bufalin	36-43	estrogen receptor 1	Homo sapiens	234-242
21259053-4	2011	In the current study, we found that bufalin, bufotalin and gamabufotalin, key members of bufadienolides isolated from a traditional Chinese medicine ChanSu, significantly potentiated human breast cancer cells with different status of ER-alpha to apoptosis induction of TRAIL, as evidenced by enhanced Annexin V/FITC positive cells (apoptotic cells), cytoplasmic histone-associated-DNA-fragments, membrane permeability transition (MPT), caspases activation and PARP cleavage.	bufalin	36-43	TNF superfamily member 10	Homo sapiens	269-274
21259053-4	2011	In the current study, we found that bufalin, bufotalin and gamabufotalin, key members of bufadienolides isolated from a traditional Chinese medicine ChanSu, significantly potentiated human breast cancer cells with different status of ER-alpha to apoptosis induction of TRAIL, as evidenced by enhanced Annexin V/FITC positive cells (apoptotic cells), cytoplasmic histone-associated-DNA-fragments, membrane permeability transition (MPT), caspases activation and PARP cleavage.	bufalin	36-43	annexin A5	Homo sapiens	301-310
21259053-4	2011	In the current study, we found that bufalin, bufotalin and gamabufotalin, key members of bufadienolides isolated from a traditional Chinese medicine ChanSu, significantly potentiated human breast cancer cells with different status of ER-alpha to apoptosis induction of TRAIL, as evidenced by enhanced Annexin V/FITC positive cells (apoptotic cells), cytoplasmic histone-associated-DNA-fragments, membrane permeability transition (MPT), caspases activation and PARP cleavage.	bufalin	36-43	collagen type XI alpha 2 chain	Homo sapiens	460-464
21259053-7	2011	The important consequence of down-regulation Mcl-1 in the enhancement action by combining bufalin with TRAIL was confirmed by either knockdown or overexpression of Mcl-1 approach.	bufalin	90-97	MCL1 apoptosis regulator, BCL2 family member	Homo sapiens	45-50
21259053-7	2011	The important consequence of down-regulation Mcl-1 in the enhancement action by combining bufalin with TRAIL was confirmed by either knockdown or overexpression of Mcl-1 approach.	bufalin	90-97	MCL1 apoptosis regulator, BCL2 family member	Homo sapiens	164-169
21181095-5	2011	Furthermore, we show that bufalin increased the phosphorylation of Akt and ERK1/2 while inhibited FoxO3a expression.	bufalin	26-33	AKT serine/threonine kinase 1	Homo sapiens	67-70
21181095-5	2011	Furthermore, we show that bufalin increased the phosphorylation of Akt and ERK1/2 while inhibited FoxO3a expression.	bufalin	26-33	mitogen-activated protein kinase 3	Homo sapiens	75-81
21181095-5	2011	Furthermore, we show that bufalin increased the phosphorylation of Akt and ERK1/2 while inhibited FoxO3a expression.	bufalin	26-33	forkhead box O3	Homo sapiens	98-104
21763418-0	2011	Bufalin induces autophagy-mediated cell death in human colon cancer cells through reactive oxygen species generation and JNK activation.	bufalin	0-7	mitogen-activated protein kinase 8	Homo sapiens	121-124
21763418-9	2011	ROS antioxidants (N-acetylcysteine and vitamin C), the JNK-specific inhibitor SP600125, and JNK2 siRNA attenuated bufalin-induced autophagy.	bufalin	114-121	mitogen-activated protein kinase 8	Homo sapiens	55-58
21763418-9	2011	ROS antioxidants (N-acetylcysteine and vitamin C), the JNK-specific inhibitor SP600125, and JNK2 siRNA attenuated bufalin-induced autophagy.	bufalin	114-121	mitogen-activated protein kinase 9	Homo sapiens	92-96
21288283-9	2011	Taken together, bufalin and cinobufagin induce apoptosis of HepG(2) cells via both Fas- and mitochondria-mediated pathways, and a Fas-mediated caspase-10-dependent pathway might play a crucial role.	bufalin	16-23	caspase 10	Homo sapiens	143-153
21719999-6	2011	Furthermore, bufalin not only inhibited upregulation of cyclin D1 and CDK4, but also downregulation of p21 in both mRNA and protein levels.	bufalin	13-20	cyclin-dependent kinase 4	Rattus norvegicus	70-74
21719999-6	2011	Furthermore, bufalin not only inhibited upregulation of cyclin D1 and CDK4, but also downregulation of p21 in both mRNA and protein levels.	bufalin	13-20	KRAS proto-oncogene, GTPase	Rattus norvegicus	103-106
21719999-7	2011	Although bufalin did not affect p27 and CDK2 mRNA expression, it reversed downregulation of p27 and upregulation of CDK2 in protein level.	bufalin	9-16	cyclin-dependent kinase inhibitor 1B	Rattus norvegicus	92-95
21719999-7	2011	Although bufalin did not affect p27 and CDK2 mRNA expression, it reversed downregulation of p27 and upregulation of CDK2 in protein level.	bufalin	9-16	cyclin dependent kinase 2	Rattus norvegicus	116-120
21719999-8	2011	Activity of CDK2 and CDK4 was also inhibited by bufalin.	bufalin	48-55	cyclin dependent kinase 2	Rattus norvegicus	12-16
21719999-8	2011	Activity of CDK2 and CDK4 was also inhibited by bufalin.	bufalin	48-55	cyclin-dependent kinase 4	Rattus norvegicus	21-25
21076398-1	2011	Here we report that bufalin and other cardiac glycoside inhibitors of the sodium-potassium ATPase (sodium pump) potently inhibit the induction of the interferon-beta (IFNbeta) gene by virus, double-stranded RNA or double-stranded DNA.	bufalin	20-27	interferon beta 1	Homo sapiens	150-165
19592481-0	2011	Bufalin Induces Reactive Oxygen Species Dependent Bax Translocation and Apoptosis in ASTC-a-1 Cells.	bufalin	0-7	BCL2 associated X, apoptosis regulator	Homo sapiens	50-53
19592481-3	2011	In this study, we used the confocal fluorescence microscopy (CFM) to monitor the spatio-temporal dynamics of reactive oxygen species (ROS) production, Bax translocation and caspase-3 activation during bufalin-induced apoptosis in living human lung adenocarcinoma (ASTC-a-1) cells.	bufalin	201-208	BCL2 associated X, apoptosis regulator	Homo sapiens	151-154
19592481-3	2011	In this study, we used the confocal fluorescence microscopy (CFM) to monitor the spatio-temporal dynamics of reactive oxygen species (ROS) production, Bax translocation and caspase-3 activation during bufalin-induced apoptosis in living human lung adenocarcinoma (ASTC-a-1) cells.	bufalin	201-208	caspase 3	Homo sapiens	173-182
19592481-5	2011	Bax redistributed from cytosol to mitochondria from 12 to 48 h after bufalin treatment in living cells expressed with green fluorescent protein Bax.	bufalin	69-76	BCL2 associated X, apoptosis regulator	Homo sapiens	0-3
19592481-5	2011	Bax redistributed from cytosol to mitochondria from 12 to 48 h after bufalin treatment in living cells expressed with green fluorescent protein Bax.	bufalin	69-76	BCL2 associated X, apoptosis regulator	Homo sapiens	144-147
19592481-6	2011	Treatment with the antioxidant N-acetyl-cysteine (NAC), a ROS scavenger, inhibited ROS generation and Bax translocation and led to a significant protection against bufalin-induced apoptosis.	bufalin	164-171	BCL2 associated X, apoptosis regulator	Homo sapiens	102-105
19592481-7	2011	Our results also revealed that bufalin induced a prominent increase of caspase-3 activation blocked potently by NAC.	bufalin	31-38	caspase 3	Homo sapiens	71-80
19592481-8	2011	Taken together, bufalin induced ROS-mediated Bax translocation, mitochondrial permeability transition and caspase-3 activation, implying that bufalin induced apoptosis via ROS-dependent mitochondrial death pathway in ASTC-a-1 cells.	bufalin	16-23	BCL2 associated X, apoptosis regulator	Homo sapiens	45-48
19592481-8	2011	Taken together, bufalin induced ROS-mediated Bax translocation, mitochondrial permeability transition and caspase-3 activation, implying that bufalin induced apoptosis via ROS-dependent mitochondrial death pathway in ASTC-a-1 cells.	bufalin	16-23	caspase 3	Homo sapiens	106-115
19592481-8	2011	Taken together, bufalin induced ROS-mediated Bax translocation, mitochondrial permeability transition and caspase-3 activation, implying that bufalin induced apoptosis via ROS-dependent mitochondrial death pathway in ASTC-a-1 cells.	bufalin	142-149	caspase 3	Homo sapiens	106-115
21076398-1	2011	Here we report that bufalin and other cardiac glycoside inhibitors of the sodium-potassium ATPase (sodium pump) potently inhibit the induction of the interferon-beta (IFNbeta) gene by virus, double-stranded RNA or double-stranded DNA.	bufalin	20-27	interferon beta 1	Homo sapiens	167-174
19429444-2	2009	The cardiotonic steroid bufalin enhances vitamin D-induced differentiation of leukemia cells and VDR transactivation activity.	bufalin	24-31	vitamin D receptor	Homo sapiens	97-100
19417737-0	2009	Bufalin inhibits CYP3A4 activity in vitro and in vivo.	bufalin	0-7	cytochrome P450 family 3 subfamily A member 4	Homo sapiens	17-23
19417737-2	2009	METHODS: Recombinant human CYP3A4 was incubated with bufalin in vitro.	bufalin	53-60	cytochrome P450 family 3 subfamily A member 4	Homo sapiens	27-33
19417737-3	2009	Bufalin was administered ig and iv to Wistar rats to further estimate its impact on CYP3A4, and midazolam was given to index the activity of CYP3A4.	bufalin	0-7	cytochrome P450 family 3 subfamily A member 4	Homo sapiens	84-90
19417737-5	2009	Bufalin affected CYP3A4 activity with increases in AUC(0-t) and t(1/2), and decreases in CL and the formation of 1-hydroxy-midazolam after ig or iv administration of midazolam (P&lt;0.05).	bufalin	0-7	cytochrome P450 family 3 subfamily A member 4	Homo sapiens	17-23
19417737-8	2009	The likelihood of an interaction between bufalin and the CYP3A4-metabolized drugs in human might not be negated.	bufalin	41-48	cytochrome P450 family 3 subfamily A member 4	Homo sapiens	57-63
20963488-8	2010	Bufalin induced the apoptosis and cell cycle arrest by affecting the protein expressions of Bcl-2/Bax, cytochrome c, caspase-3, PARP, p53, p21WAF1, cyclinD1, and COX-2 in A549 cells.	bufalin	0-7	BCL2 apoptosis regulator	Homo sapiens	92-97
20963488-8	2010	Bufalin induced the apoptosis and cell cycle arrest by affecting the protein expressions of Bcl-2/Bax, cytochrome c, caspase-3, PARP, p53, p21WAF1, cyclinD1, and COX-2 in A549 cells.	bufalin	0-7	BCL2 associated X, apoptosis regulator	Homo sapiens	98-101
20963488-8	2010	Bufalin induced the apoptosis and cell cycle arrest by affecting the protein expressions of Bcl-2/Bax, cytochrome c, caspase-3, PARP, p53, p21WAF1, cyclinD1, and COX-2 in A549 cells.	bufalin	0-7	cytochrome c, somatic	Homo sapiens	103-115
20963488-8	2010	Bufalin induced the apoptosis and cell cycle arrest by affecting the protein expressions of Bcl-2/Bax, cytochrome c, caspase-3, PARP, p53, p21WAF1, cyclinD1, and COX-2 in A549 cells.	bufalin	0-7	caspase 3	Homo sapiens	117-126
20963488-8	2010	Bufalin induced the apoptosis and cell cycle arrest by affecting the protein expressions of Bcl-2/Bax, cytochrome c, caspase-3, PARP, p53, p21WAF1, cyclinD1, and COX-2 in A549 cells.	bufalin	0-7	collagen type XI alpha 2 chain	Homo sapiens	128-132
20963488-8	2010	Bufalin induced the apoptosis and cell cycle arrest by affecting the protein expressions of Bcl-2/Bax, cytochrome c, caspase-3, PARP, p53, p21WAF1, cyclinD1, and COX-2 in A549 cells.	bufalin	0-7	tumor protein p53	Homo sapiens	134-137
20963488-8	2010	Bufalin induced the apoptosis and cell cycle arrest by affecting the protein expressions of Bcl-2/Bax, cytochrome c, caspase-3, PARP, p53, p21WAF1, cyclinD1, and COX-2 in A549 cells.	bufalin	0-7	cyclin D1	Homo sapiens	148-156
20963488-8	2010	Bufalin induced the apoptosis and cell cycle arrest by affecting the protein expressions of Bcl-2/Bax, cytochrome c, caspase-3, PARP, p53, p21WAF1, cyclinD1, and COX-2 in A549 cells.	bufalin	0-7	mitochondrially encoded cytochrome c oxidase II	Homo sapiens	162-167
20963488-10	2010	Furthermore, bufalin inhibited the protein expressions and phosphorylation of Akt, NF-kappaB, p44/42 MAPK (ERK1/2) and p38 MAPK in A549 cells.	bufalin	13-20	AKT serine/threonine kinase 1	Homo sapiens	78-81
20963488-10	2010	Furthermore, bufalin inhibited the protein expressions and phosphorylation of Akt, NF-kappaB, p44/42 MAPK (ERK1/2) and p38 MAPK in A549 cells.	bufalin	13-20	nuclear factor kappa B subunit 1	Homo sapiens	83-92
20963488-10	2010	Furthermore, bufalin inhibited the protein expressions and phosphorylation of Akt, NF-kappaB, p44/42 MAPK (ERK1/2) and p38 MAPK in A549 cells.	bufalin	13-20	interferon induced protein 44	Homo sapiens	94-97
20963488-10	2010	Furthermore, bufalin inhibited the protein expressions and phosphorylation of Akt, NF-kappaB, p44/42 MAPK (ERK1/2) and p38 MAPK in A549 cells.	bufalin	13-20	mitogen-activated protein kinase 3	Homo sapiens	107-113
20963488-10	2010	Furthermore, bufalin inhibited the protein expressions and phosphorylation of Akt, NF-kappaB, p44/42 MAPK (ERK1/2) and p38 MAPK in A549 cells.	bufalin	13-20	mitogen-activated protein kinase 1	Homo sapiens	119-122
20963488-11	2010	Our results suggest that bufalin inhibits the human lung cancer cell proliferation via VEGFR1/VEGFR2/EGFR/c-Met-Akt/p44/42/p38-NF-kappaB signaling pathways; bufalin may have a wide therapeutic and/or adjuvant therapeutic application in the treatment of human NSCLC.	bufalin	25-32	fms related receptor tyrosine kinase 1	Homo sapiens	87-93
20963488-11	2010	Our results suggest that bufalin inhibits the human lung cancer cell proliferation via VEGFR1/VEGFR2/EGFR/c-Met-Akt/p44/42/p38-NF-kappaB signaling pathways; bufalin may have a wide therapeutic and/or adjuvant therapeutic application in the treatment of human NSCLC.	bufalin	25-32	kinase insert domain receptor	Homo sapiens	94-100
20963488-11	2010	Our results suggest that bufalin inhibits the human lung cancer cell proliferation via VEGFR1/VEGFR2/EGFR/c-Met-Akt/p44/42/p38-NF-kappaB signaling pathways; bufalin may have a wide therapeutic and/or adjuvant therapeutic application in the treatment of human NSCLC.	bufalin	25-32	epidermal growth factor receptor	Homo sapiens	88-92
20963488-11	2010	Our results suggest that bufalin inhibits the human lung cancer cell proliferation via VEGFR1/VEGFR2/EGFR/c-Met-Akt/p44/42/p38-NF-kappaB signaling pathways; bufalin may have a wide therapeutic and/or adjuvant therapeutic application in the treatment of human NSCLC.	bufalin	25-32	MET proto-oncogene, receptor tyrosine kinase	Homo sapiens	106-111
20963488-11	2010	Our results suggest that bufalin inhibits the human lung cancer cell proliferation via VEGFR1/VEGFR2/EGFR/c-Met-Akt/p44/42/p38-NF-kappaB signaling pathways; bufalin may have a wide therapeutic and/or adjuvant therapeutic application in the treatment of human NSCLC.	bufalin	25-32	AKT serine/threonine kinase 1	Homo sapiens	112-115
20963488-11	2010	Our results suggest that bufalin inhibits the human lung cancer cell proliferation via VEGFR1/VEGFR2/EGFR/c-Met-Akt/p44/42/p38-NF-kappaB signaling pathways; bufalin may have a wide therapeutic and/or adjuvant therapeutic application in the treatment of human NSCLC.	bufalin	25-32	interferon induced protein 44	Homo sapiens	116-119
20963488-11	2010	Our results suggest that bufalin inhibits the human lung cancer cell proliferation via VEGFR1/VEGFR2/EGFR/c-Met-Akt/p44/42/p38-NF-kappaB signaling pathways; bufalin may have a wide therapeutic and/or adjuvant therapeutic application in the treatment of human NSCLC.	bufalin	25-32	mitogen-activated protein kinase 1	Homo sapiens	123-126
20963488-11	2010	Our results suggest that bufalin inhibits the human lung cancer cell proliferation via VEGFR1/VEGFR2/EGFR/c-Met-Akt/p44/42/p38-NF-kappaB signaling pathways; bufalin may have a wide therapeutic and/or adjuvant therapeutic application in the treatment of human NSCLC.	bufalin	25-32	nuclear factor kappa B subunit 1	Homo sapiens	127-136
21163061-8	2010	The bufalin-induced apoptosis was confirmed by increased expression of tumor suppressor protein p53, bax and decreased expression of bcl-2.	bufalin	4-11	tumor protein p53	Homo sapiens	96-99
21163061-8	2010	The bufalin-induced apoptosis was confirmed by increased expression of tumor suppressor protein p53, bax and decreased expression of bcl-2.	bufalin	4-11	BCL2 associated X, apoptosis regulator	Homo sapiens	101-104
21163061-8	2010	The bufalin-induced apoptosis was confirmed by increased expression of tumor suppressor protein p53, bax and decreased expression of bcl-2.	bufalin	4-11	BCL2 apoptosis regulator	Homo sapiens	133-138
20840810-6	2010	The expression of Livin protein was downregulated (P &lt; 0.01) and Caspase-3 protein was activated during Bufalin-induced apoptosis in A549 cells.	bufalin	107-114	baculoviral IAP repeat containing 7	Homo sapiens	18-23
20840810-6	2010	The expression of Livin protein was downregulated (P &lt; 0.01) and Caspase-3 protein was activated during Bufalin-induced apoptosis in A549 cells.	bufalin	107-114	caspase 3	Homo sapiens	68-77
19132366-5	2009	The emission peak at 526 nm disappeared and that of 476 nm increased after STS or bufalin treatment, but taxol treatment did not induce a significant change for the SCAT3 emission spectra, indicating that caspase-3 was activated during STS- or bufalin-induced cell apoptosis, but was not involved in taxol-induced PCD.	bufalin	82-89	caspase 3	Homo sapiens	205-214
19132366-5	2009	The emission peak at 526 nm disappeared and that of 476 nm increased after STS or bufalin treatment, but taxol treatment did not induce a significant change for the SCAT3 emission spectra, indicating that caspase-3 was activated during STS- or bufalin-induced cell apoptosis, but was not involved in taxol-induced PCD.	bufalin	244-251	caspase 3	Homo sapiens	205-214
19429444-10	2009	Bufalin-induced nuclear VDR expression was associated with histone acetylation and VDR recruitment to the CYP24A1 promoter in HL60 cells.	bufalin	0-7	vitamin D receptor	Homo sapiens	24-27
19429444-10	2009	Bufalin-induced nuclear VDR expression was associated with histone acetylation and VDR recruitment to the CYP24A1 promoter in HL60 cells.	bufalin	0-7	vitamin D receptor	Homo sapiens	83-86
19429444-10	2009	Bufalin-induced nuclear VDR expression was associated with histone acetylation and VDR recruitment to the CYP24A1 promoter in HL60 cells.	bufalin	0-7	cytochrome P450 family 24 subfamily A member 1	Homo sapiens	106-113
19429444-11	2009	Thus, the Na(+),K(+)-ATPase inhibitor bufalin modulates VDR function through several mechanisms, including Erk MAP kinase activation and increased nuclear VDR expression.	bufalin	38-45	vitamin D receptor	Homo sapiens	56-59
19429444-11	2009	Thus, the Na(+),K(+)-ATPase inhibitor bufalin modulates VDR function through several mechanisms, including Erk MAP kinase activation and increased nuclear VDR expression.	bufalin	38-45	mitogen-activated protein kinase 1	Homo sapiens	107-110
19429444-11	2009	Thus, the Na(+),K(+)-ATPase inhibitor bufalin modulates VDR function through several mechanisms, including Erk MAP kinase activation and increased nuclear VDR expression.	bufalin	38-45	vitamin D receptor	Homo sapiens	155-158
19429444-4	2009	Bufalin in combination with 1,25(OH)(2)D(3) enhanced the expression of VDR target genes, such as CYP24A1 and cathelicidin antimicrobial peptide, and effectively induced differentiation phenotypes.	bufalin	0-7	vitamin D receptor	Homo sapiens	71-74
19429444-4	2009	Bufalin in combination with 1,25(OH)(2)D(3) enhanced the expression of VDR target genes, such as CYP24A1 and cathelicidin antimicrobial peptide, and effectively induced differentiation phenotypes.	bufalin	0-7	cytochrome P450 family 24 subfamily A member 1	Homo sapiens	97-104
19429444-4	2009	Bufalin in combination with 1,25(OH)(2)D(3) enhanced the expression of VDR target genes, such as CYP24A1 and cathelicidin antimicrobial peptide, and effectively induced differentiation phenotypes.	bufalin	0-7	cathelicidin antimicrobial peptide	Homo sapiens	109-143
19429444-7	2009	Interestingly, bufalin enhanced 1,25(OH)(2)D(3)-induced nuclear VDR expression.	bufalin	15-22	vitamin D receptor	Homo sapiens	64-67
19037992-13	2008	These results suggest that bufalin and cinobufagin suppress cell proliferation and cause apoptosis in prostate cancer cells via a sequence of apoptotic modulators, including Bax, cytochrome c, and caspases.	bufalin	27-34	BCL2 associated X, apoptosis regulator	Homo sapiens	174-177
19236749-0	2009	[Effects of Bufalin on SYK and CBL family proteins in induction of HL-60 cell apoptosis].	bufalin	12-19	spleen associated tyrosine kinase	Homo sapiens	23-26
19236749-0	2009	[Effects of Bufalin on SYK and CBL family proteins in induction of HL-60 cell apoptosis].	bufalin	12-19	Cbl proto-oncogene	Homo sapiens	31-34
19236749-1	2009	The study was aimed to explore the mechanism of SYK and CBL family of ubiquitin ligases in Bufalin-induced HL-60 cells apoptosis.	bufalin	91-98	spleen associated tyrosine kinase	Homo sapiens	48-51
19236749-1	2009	The study was aimed to explore the mechanism of SYK and CBL family of ubiquitin ligases in Bufalin-induced HL-60 cells apoptosis.	bufalin	91-98	Cbl proto-oncogene	Homo sapiens	56-59
19236749-7	2009	SYK was quickly phosphorylated, and the expressions of CBL and CBL-b were down-regulated after treatment with Bufalin.	bufalin	110-117	spleen associated tyrosine kinase	Homo sapiens	0-3
19236749-7	2009	SYK was quickly phosphorylated, and the expressions of CBL and CBL-b were down-regulated after treatment with Bufalin.	bufalin	110-117	Cbl proto-oncogene	Homo sapiens	55-58
19236749-7	2009	SYK was quickly phosphorylated, and the expressions of CBL and CBL-b were down-regulated after treatment with Bufalin.	bufalin	110-117	Cbl proto-oncogene B	Homo sapiens	63-68
19343001-0	2009	PI3K/Akt is involved in bufalin-induced apoptosis in gastric cancer cells.	bufalin	24-31	AKT serine/threonine kinase 1	Homo sapiens	5-8
19343001-5	2009	Bufalin increased the Bax/Bcl-2 ratio and activated caspase-3 during the apoptotic process of MGC803 cells.	bufalin	0-7	BCL2 associated X, apoptosis regulator	Homo sapiens	22-25
19343001-5	2009	Bufalin increased the Bax/Bcl-2 ratio and activated caspase-3 during the apoptotic process of MGC803 cells.	bufalin	0-7	BCL2 apoptosis regulator	Homo sapiens	26-31
19343001-5	2009	Bufalin increased the Bax/Bcl-2 ratio and activated caspase-3 during the apoptotic process of MGC803 cells.	bufalin	0-7	caspase 3	Homo sapiens	52-61
19343001-6	2009	It should be noted that bufalin transiently activated the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway and then inhibited it completely, and upregulated the Casitas B-lineage lymphoma (Cbl) family of ubiquitin ligases, upstream modulators of PI3K.	bufalin	24-31	AKT serine/threonine kinase 1	Homo sapiens	95-98
19343001-6	2009	It should be noted that bufalin transiently activated the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway and then inhibited it completely, and upregulated the Casitas B-lineage lymphoma (Cbl) family of ubiquitin ligases, upstream modulators of PI3K.	bufalin	24-31	Cbl proto-oncogene	Homo sapiens	171-197
19343001-6	2009	It should be noted that bufalin transiently activated the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway and then inhibited it completely, and upregulated the Casitas B-lineage lymphoma (Cbl) family of ubiquitin ligases, upstream modulators of PI3K.	bufalin	24-31	Cbl proto-oncogene	Homo sapiens	199-202
19343001-8	2009	These results suggested that the PI3K/Akt pathway might play a key role in bufalin-induced apoptosis in gastric cancer MGC803 cells.	bufalin	75-82	AKT serine/threonine kinase 1	Homo sapiens	38-41
19037992-13	2008	These results suggest that bufalin and cinobufagin suppress cell proliferation and cause apoptosis in prostate cancer cells via a sequence of apoptotic modulators, including Bax, cytochrome c, and caspases.	bufalin	27-34	cytochrome c, somatic	Homo sapiens	179-191
19037992-13	2008	These results suggest that bufalin and cinobufagin suppress cell proliferation and cause apoptosis in prostate cancer cells via a sequence of apoptotic modulators, including Bax, cytochrome c, and caspases.	bufalin	27-34	caspase 8	Homo sapiens	197-205
17352239-2	2007	Tiam1 was also found to play a critical role in bufalin-induced apoptosis through the activation of the Rac1, PAK and JNK pathway in human leukemia cell lines.	bufalin	48-55	TIAM Rac1 associated GEF 1	Homo sapiens	0-5
19176038-3	2008	RESULTS: The combination of 5 nmol/L bufalin and 30 nmol/L ATRA induced HL-60 cells differentiation in a time-dependent manner, the percentages of CD11b positive cells treated for 2 d and 4 d being (20.0 +/- 2.8)% and (75.0 +/- 5.3)%, respectively, with the increasing of cellular adherence ability.	bufalin	37-44	integrin subunit alpha M	Homo sapiens	147-152
17659679-4	2007	Bufalin was injected intraperitoneally into three groups at doses of 1.5 mg/kg (BF1), 1 mg/kg (BF2) and 0.5 mg/kg (BF3) for d 15-24, respectively.	bufalin	0-7	forkhead box G1	Homo sapiens	80-83
17659679-4	2007	Bufalin was injected intraperitoneally into three groups at doses of 1.5 mg/kg (BF1), 1 mg/kg (BF2) and 0.5 mg/kg (BF3) for d 15-24, respectively.	bufalin	0-7	forkhead box G1	Homo sapiens	95-98
18164258-0	2008	Up-regulation of the Cbl family of ubiquitin ligases is involved in ATRA and bufalin-induced cell adhesion but not cell differentiation.	bufalin	77-84	Cbl proto-oncogene	Homo sapiens	21-24
18164258-4	2008	In the present study, we demonstrated that bufalin enhanced all-trans retinoic acid (ATRA) induced differentiation of HL-60 cells, accompanied by up-regulation of the Cbl family of ubiquitin ligases.	bufalin	43-50	Cbl proto-oncogene	Homo sapiens	167-170
18164258-9	2008	These results suggested that up-regulation of c-Cbl and Cbl-b was involved in the regulation of ATRA and bufalin-induced HL-60 cell adhesion rather than cell differentiation, which might be mediated by lipid raft localization, ubiquitin ligase activity and C-terminal structure of Cbl proteins.	bufalin	105-112	Cbl proto-oncogene	Homo sapiens	46-51
18164258-9	2008	These results suggested that up-regulation of c-Cbl and Cbl-b was involved in the regulation of ATRA and bufalin-induced HL-60 cell adhesion rather than cell differentiation, which might be mediated by lipid raft localization, ubiquitin ligase activity and C-terminal structure of Cbl proteins.	bufalin	105-112	Cbl proto-oncogene B	Homo sapiens	56-61
18164258-9	2008	These results suggested that up-regulation of c-Cbl and Cbl-b was involved in the regulation of ATRA and bufalin-induced HL-60 cell adhesion rather than cell differentiation, which might be mediated by lipid raft localization, ubiquitin ligase activity and C-terminal structure of Cbl proteins.	bufalin	105-112	Cbl proto-oncogene	Homo sapiens	48-51
17439728-5	2007	The effect of bufalin on dihydrofolate reductase (DHFR) expression was studied by RTPCR and Western blotting.	bufalin	14-21	dihydrofolate reductase	Homo sapiens	50-54
17490524-0	2007	[Effect of bufalin-inducing apoptosis on Bcl-2 and PKC in HL-60 cells].	bufalin	11-18	BCL2 apoptosis regulator	Homo sapiens	41-46
17490524-0	2007	[Effect of bufalin-inducing apoptosis on Bcl-2 and PKC in HL-60 cells].	bufalin	11-18	proline rich transmembrane protein 2	Homo sapiens	51-54
17490524-3	2007	The present study was designed to investigate the effects of bufalin-induced apoptosis on Bcl-2 and PKC in human leukemic HL-60 cells.	bufalin	61-68	BCL2 apoptosis regulator	Homo sapiens	90-95
17490524-3	2007	The present study was designed to investigate the effects of bufalin-induced apoptosis on Bcl-2 and PKC in human leukemic HL-60 cells.	bufalin	61-68	proline rich transmembrane protein 2	Homo sapiens	100-103
17490524-9	2007	(3) compared with control, treatment with bufalin at concentration of 50 nmol/L for 6 - 24 hours resulted in downregulation of protein expression, decrease of phosphorylation, and cleavage of Bcl-2, simultaneously.	bufalin	42-49	BCL2 apoptosis regulator	Homo sapiens	192-197
17490524-10	2007	(4) the activity of total PKC was unchanged when HL-60 cells were exposed to 1 - 100 nmol/L bufalin for 30 minutes, but PKCbetaII underwent translocation from cytosol to membrane.	bufalin	92-99	proline rich transmembrane protein 2	Homo sapiens	26-29
17352239-2	2007	Tiam1 was also found to play a critical role in bufalin-induced apoptosis through the activation of the Rac1, PAK and JNK pathway in human leukemia cell lines.	bufalin	48-55	Rac family small GTPase 1	Homo sapiens	104-108
17352239-2	2007	Tiam1 was also found to play a critical role in bufalin-induced apoptosis through the activation of the Rac1, PAK and JNK pathway in human leukemia cell lines.	bufalin	48-55	mitogen-activated protein kinase 8	Homo sapiens	118-121
17352239-7	2007	Expression of both Tiam1 mRNA and its protein was induced 0.5 h after the start of the bufalin treatment.	bufalin	87-94	TIAM Rac1 associated GEF 1	Homo sapiens	19-24
17352239-8	2007	Transfection of Tiam1-specific siRNA into HeLa cells markedly inhibited bufalin-induced apoptosis.	bufalin	72-79	TIAM Rac1 associated GEF 1	Homo sapiens	16-21
17352239-9	2007	CONCLUSION: Our results suggest that Tiam1 is a downstream mediator of bufalin-induced apoptosis in the human solid tumor HeLa cell line, as well as in leukemia cell lines.	bufalin	71-78	TIAM Rac1 associated GEF 1	Homo sapiens	37-42
16183038-4	2005	Bufalin also augmented VDR activation by bile acid ligands, such as lithocholic acid and 3-ketocholanic acid.	bufalin	0-7	vitamin D receptor	Homo sapiens	23-26
16732934-1	2006	OBJECTIVE: To investigate the expression of mitochondria-mediated apoptosis pathway related genes bcl-2, Bax, survivin and Smac/DIABLO in bufalin induced HL-60 cell apoptosis.	bufalin	138-145	BCL2 apoptosis regulator	Homo sapiens	98-103
16732934-1	2006	OBJECTIVE: To investigate the expression of mitochondria-mediated apoptosis pathway related genes bcl-2, Bax, survivin and Smac/DIABLO in bufalin induced HL-60 cell apoptosis.	bufalin	138-145	BCL2 associated X, apoptosis regulator	Homo sapiens	105-108
16732934-1	2006	OBJECTIVE: To investigate the expression of mitochondria-mediated apoptosis pathway related genes bcl-2, Bax, survivin and Smac/DIABLO in bufalin induced HL-60 cell apoptosis.	bufalin	138-145	diablo IAP-binding mitochondrial protein	Homo sapiens	123-127
16732934-1	2006	OBJECTIVE: To investigate the expression of mitochondria-mediated apoptosis pathway related genes bcl-2, Bax, survivin and Smac/DIABLO in bufalin induced HL-60 cell apoptosis.	bufalin	138-145	diablo IAP-binding mitochondrial protein	Homo sapiens	128-134
16732934-7	2006	The active subunits of caspase-3 were displayed after treatment for 8 h till 48 h. CONCLUSION: Apoptosis induced by bufalin is related to downregulation of expressions of bcl-2 and survivin, decrease of Bcl-2/Bax ratio, mitochondrial release of Smac/DIABLO, and activation of caspase-3.	bufalin	116-123	caspase 3	Homo sapiens	23-32
16732934-7	2006	The active subunits of caspase-3 were displayed after treatment for 8 h till 48 h. CONCLUSION: Apoptosis induced by bufalin is related to downregulation of expressions of bcl-2 and survivin, decrease of Bcl-2/Bax ratio, mitochondrial release of Smac/DIABLO, and activation of caspase-3.	bufalin	116-123	BCL2 apoptosis regulator	Homo sapiens	171-176
16732934-7	2006	The active subunits of caspase-3 were displayed after treatment for 8 h till 48 h. CONCLUSION: Apoptosis induced by bufalin is related to downregulation of expressions of bcl-2 and survivin, decrease of Bcl-2/Bax ratio, mitochondrial release of Smac/DIABLO, and activation of caspase-3.	bufalin	116-123	BCL2 apoptosis regulator	Homo sapiens	203-208
16732934-7	2006	The active subunits of caspase-3 were displayed after treatment for 8 h till 48 h. CONCLUSION: Apoptosis induced by bufalin is related to downregulation of expressions of bcl-2 and survivin, decrease of Bcl-2/Bax ratio, mitochondrial release of Smac/DIABLO, and activation of caspase-3.	bufalin	116-123	BCL2 associated X, apoptosis regulator	Homo sapiens	209-212
16732934-7	2006	The active subunits of caspase-3 were displayed after treatment for 8 h till 48 h. CONCLUSION: Apoptosis induced by bufalin is related to downregulation of expressions of bcl-2 and survivin, decrease of Bcl-2/Bax ratio, mitochondrial release of Smac/DIABLO, and activation of caspase-3.	bufalin	116-123	diablo IAP-binding mitochondrial protein	Homo sapiens	245-249
16732934-7	2006	The active subunits of caspase-3 were displayed after treatment for 8 h till 48 h. CONCLUSION: Apoptosis induced by bufalin is related to downregulation of expressions of bcl-2 and survivin, decrease of Bcl-2/Bax ratio, mitochondrial release of Smac/DIABLO, and activation of caspase-3.	bufalin	116-123	diablo IAP-binding mitochondrial protein	Homo sapiens	250-256
16732934-7	2006	The active subunits of caspase-3 were displayed after treatment for 8 h till 48 h. CONCLUSION: Apoptosis induced by bufalin is related to downregulation of expressions of bcl-2 and survivin, decrease of Bcl-2/Bax ratio, mitochondrial release of Smac/DIABLO, and activation of caspase-3.	bufalin	116-123	caspase 3	Homo sapiens	276-285
16780682-7	2006	RESULTS: Bufalin combined with ATRA can induce differentiation of APL cells towards mature stages, NBT reduction was increased 15% - 52% and CD(11)b expression was also increased 16% - 69% in combination of bufalin and ATRA as compared with that of ATRA alone, while the concentration of ATRA needed in the combination group was reduced to 30% and the time of differentiation was reduced from 7 days to 4 days.	bufalin	9-16	integrin subunit alpha M	Homo sapiens	141-148
16780682-7	2006	RESULTS: Bufalin combined with ATRA can induce differentiation of APL cells towards mature stages, NBT reduction was increased 15% - 52% and CD(11)b expression was also increased 16% - 69% in combination of bufalin and ATRA as compared with that of ATRA alone, while the concentration of ATRA needed in the combination group was reduced to 30% and the time of differentiation was reduced from 7 days to 4 days.	bufalin	207-214	integrin subunit alpha M	Homo sapiens	141-148
16183038-6	2005	Bufalin did not bind directly to VDR but did modulate the interaction of VDR and cofactors, such as steroid receptor coactivator-1 and nuclear receptor corepressor.	bufalin	0-7	vitamin D receptor	Homo sapiens	73-76
16183038-7	2005	Bufalin treatment significantly increased the expression of an endogenous VDR target gene, CYP24, in kidney- and monocyte-derived cell lines treated with 1,25(OH)(2)D(3).	bufalin	0-7	vitamin D receptor	Homo sapiens	74-77
16183038-7	2005	Bufalin treatment significantly increased the expression of an endogenous VDR target gene, CYP24, in kidney- and monocyte-derived cell lines treated with 1,25(OH)(2)D(3).	bufalin	0-7	cytochrome P450 family 24 subfamily A member 1	Homo sapiens	91-96
16183038-9	2005	Bufalin may be a useful tool in the investigation of VDR regulation by membrane-originating cellular signals and of pathophysiological mechanisms linking VDR to cardiovascular dysfunction.	bufalin	0-7	vitamin D receptor	Homo sapiens	53-56
16183038-9	2005	Bufalin may be a useful tool in the investigation of VDR regulation by membrane-originating cellular signals and of pathophysiological mechanisms linking VDR to cardiovascular dysfunction.	bufalin	0-7	vitamin D receptor	Homo sapiens	154-157
15494417-4	2005	At a concentration of 1 microM, ouabain and other tested cardenolides, as well as bufadienolides such as bufalin, cinobufagin, cinobufotalin, and telobufotoxin, led to approximately 10-fold inhibition of the Na(+),K(+) pump, a 2-3-fold decrease in staining with dimethylthiazol-diphenyltetrazolium (MTT), and massive death indicated by detachment of approximately 80% of cells and caspase-3 activation.	bufalin	105-112	caspase 3	Canis lupus familiaris	381-390
16390854-8	2005	The down-regulation of the cyclin A, Bcl-2, and Bcl-X(L) expression with the simultaneous up-regulation of the p21 and Bax expression, and caspase-9 activation was observed in ECSC after bufalin treatment.	bufalin	187-194	cyclin A2	Homo sapiens	27-35
16390854-8	2005	The down-regulation of the cyclin A, Bcl-2, and Bcl-X(L) expression with the simultaneous up-regulation of the p21 and Bax expression, and caspase-9 activation was observed in ECSC after bufalin treatment.	bufalin	187-194	BCL2 apoptosis regulator	Homo sapiens	37-42
16390854-8	2005	The down-regulation of the cyclin A, Bcl-2, and Bcl-X(L) expression with the simultaneous up-regulation of the p21 and Bax expression, and caspase-9 activation was observed in ECSC after bufalin treatment.	bufalin	187-194	BCL2 like 1	Homo sapiens	48-56
16390854-8	2005	The down-regulation of the cyclin A, Bcl-2, and Bcl-X(L) expression with the simultaneous up-regulation of the p21 and Bax expression, and caspase-9 activation was observed in ECSC after bufalin treatment.	bufalin	187-194	H3 histone pseudogene 16	Homo sapiens	111-114
16390854-8	2005	The down-regulation of the cyclin A, Bcl-2, and Bcl-X(L) expression with the simultaneous up-regulation of the p21 and Bax expression, and caspase-9 activation was observed in ECSC after bufalin treatment.	bufalin	187-194	BCL2 associated X, apoptosis regulator	Homo sapiens	119-122
16390854-8	2005	The down-regulation of the cyclin A, Bcl-2, and Bcl-X(L) expression with the simultaneous up-regulation of the p21 and Bax expression, and caspase-9 activation was observed in ECSC after bufalin treatment.	bufalin	187-194	caspase 9	Homo sapiens	139-148
11171564-3	2001	A PKC-specific but isozyme-nonselective inhibitor, Ro-31-8220, and a cPKC selective inhibitor, Go-6976, caused significant attenuation of bufalin-induced interleukin-1beta (IL-1beta) gene expression, a mature monocytic marker, indicating that cPKC participates in the bufalin-induced cell differentiation.	bufalin	138-145	interleukin 1 beta	Homo sapiens	154-171
14982968-8	2004	The antihypertensive effect of digoxin-specific Fab seen in preeclampsia and animal models of hypertension may therefore be due to a molecule identical to or structurally similar to ouabain or bufalin.	bufalin	193-200	FA complementation group B	Homo sapiens	48-51
15261311-4	2004	Compounds 3, 4, 9 and 11-14 are new mono- or dihydroxylated derivatives of bufalin with novel oxyfunctionalities at C-1beta, C-7beta, C-11beta, C-12beta and C-16alpha positions.	bufalin	75-82	endogenous retrovirus group K member 14	Homo sapiens	125-132
15261311-4	2004	Compounds 3, 4, 9 and 11-14 are new mono- or dihydroxylated derivatives of bufalin with novel oxyfunctionalities at C-1beta, C-7beta, C-11beta, C-12beta and C-16alpha positions.	bufalin	75-82	endogenous retrovirus group K member 20	Homo sapiens	134-142
12497584-2	2003	This study was to evaluate bufalin and cinobufagin effects on the proliferation of prostate cancer cell lines named LNCaP, DU145, and PC3.	bufalin	27-34	chromobox 8	Homo sapiens	134-137
12497584-10	2003	Bufalin or cinobufagin increased [Ca(2+)](i) and apoptosis in cancer cells after a 24-hr culture as well as caspase 3 activities in DU145 and PC3 cells and caspase 9 activities in LNCaP cells.	bufalin	0-7	caspase 3	Homo sapiens	108-117
12497584-10	2003	Bufalin or cinobufagin increased [Ca(2+)](i) and apoptosis in cancer cells after a 24-hr culture as well as caspase 3 activities in DU145 and PC3 cells and caspase 9 activities in LNCaP cells.	bufalin	0-7	chromobox 8	Homo sapiens	142-145
12497584-10	2003	Bufalin or cinobufagin increased [Ca(2+)](i) and apoptosis in cancer cells after a 24-hr culture as well as caspase 3 activities in DU145 and PC3 cells and caspase 9 activities in LNCaP cells.	bufalin	0-7	caspase 9	Homo sapiens	156-165
12411052-1	2002	OBJECTIVE: To study the change of WT1 gene expression during human leukemic K562 cell differentiation and apoptosis induced by bufalin.	bufalin	127-134	WT1 transcription factor	Homo sapiens	34-37
12411052-5	2002	(3) The expression of WT1 protein and mRNA were downregulated by bufalin in the initial stage of differentiation and apoptosis induced by bufalin.	bufalin	65-72	WT1 transcription factor	Homo sapiens	22-25
12411052-5	2002	(3) The expression of WT1 protein and mRNA were downregulated by bufalin in the initial stage of differentiation and apoptosis induced by bufalin.	bufalin	138-145	WT1 transcription factor	Homo sapiens	22-25
11171564-3	2001	A PKC-specific but isozyme-nonselective inhibitor, Ro-31-8220, and a cPKC selective inhibitor, Go-6976, caused significant attenuation of bufalin-induced interleukin-1beta (IL-1beta) gene expression, a mature monocytic marker, indicating that cPKC participates in the bufalin-induced cell differentiation.	bufalin	138-145	interleukin 1 beta	Homo sapiens	173-181
9781623-3	1998	Recently, we found that administration of bufalin in male rats diminished the luteinizing hormone (LH) response to gonadotropin-releasing hormone (GnRH) and the secretion of testosterone both in vivo and in vitro.	bufalin	42-49	gonadotropin releasing hormone 1	Rattus norvegicus	115-145
11016820-7	2000	RT-PCR of additional 14-3-3 isoforms revealed that the eta and gamma isoforms of 14-3-3 are also down-regulated by ouabain, bufalin and 19-norbufalin derivative, whereas the zeta isoform is down-regulated only by bufalin.	bufalin	124-131	endothelin receptor type A	Homo sapiens	55-58
11016820-7	2000	RT-PCR of additional 14-3-3 isoforms revealed that the eta and gamma isoforms of 14-3-3 are also down-regulated by ouabain, bufalin and 19-norbufalin derivative, whereas the zeta isoform is down-regulated only by bufalin.	bufalin	142-149	endothelin receptor type A	Homo sapiens	55-58
10712238-1	2000	Treatment of human leukemia THP-1 cells with bufalin, a specific inhibitor of Na(+)-K(+)-ATPase, sequentially induces c-fos and inflammatory cytokines interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) gene expressions before the appearance of mature phenotypes of monocytic cells.	bufalin	45-52	Fos proto-oncogene, AP-1 transcription factor subunit	Homo sapiens	118-123
10712238-1	2000	Treatment of human leukemia THP-1 cells with bufalin, a specific inhibitor of Na(+)-K(+)-ATPase, sequentially induces c-fos and inflammatory cytokines interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) gene expressions before the appearance of mature phenotypes of monocytic cells.	bufalin	45-52	interleukin 1 beta	Homo sapiens	151-169
10712238-1	2000	Treatment of human leukemia THP-1 cells with bufalin, a specific inhibitor of Na(+)-K(+)-ATPase, sequentially induces c-fos and inflammatory cytokines interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) gene expressions before the appearance of mature phenotypes of monocytic cells.	bufalin	45-52	interleukin 1 beta	Homo sapiens	171-180
10712238-1	2000	Treatment of human leukemia THP-1 cells with bufalin, a specific inhibitor of Na(+)-K(+)-ATPase, sequentially induces c-fos and inflammatory cytokines interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) gene expressions before the appearance of mature phenotypes of monocytic cells.	bufalin	45-52	tumor necrosis factor	Homo sapiens	186-213
10712238-1	2000	Treatment of human leukemia THP-1 cells with bufalin, a specific inhibitor of Na(+)-K(+)-ATPase, sequentially induces c-fos and inflammatory cytokines interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) gene expressions before the appearance of mature phenotypes of monocytic cells.	bufalin	45-52	tumor necrosis factor	Homo sapiens	215-224
10712238-3	2000	Bufalin selectively activated extracellular signal-regulated kinase (ERK), compared with other mitogen-activated protein (MAP) kinase family members.	bufalin	0-7	mitogen-activated protein kinase 1	Homo sapiens	30-67
10712238-3	2000	Bufalin selectively activated extracellular signal-regulated kinase (ERK), compared with other mitogen-activated protein (MAP) kinase family members.	bufalin	0-7	mitogen-activated protein kinase 1	Homo sapiens	69-72
10712238-4	2000	Pretreatment of THP-1 cells with PD-98059, an inhibitor of the ERK-kinase cascade, abolished bufalin-induced c-fos and IL-1 beta gene expressions, indicating that the ERK-kinase cascade mediates the induction of inflammatory cytokines by bufalin.	bufalin	93-100	mitogen-activated protein kinase 1	Homo sapiens	63-66
10712238-4	2000	Pretreatment of THP-1 cells with PD-98059, an inhibitor of the ERK-kinase cascade, abolished bufalin-induced c-fos and IL-1 beta gene expressions, indicating that the ERK-kinase cascade mediates the induction of inflammatory cytokines by bufalin.	bufalin	93-100	Fos proto-oncogene, AP-1 transcription factor subunit	Homo sapiens	109-114
10712238-4	2000	Pretreatment of THP-1 cells with PD-98059, an inhibitor of the ERK-kinase cascade, abolished bufalin-induced c-fos and IL-1 beta gene expressions, indicating that the ERK-kinase cascade mediates the induction of inflammatory cytokines by bufalin.	bufalin	93-100	interleukin 1 beta	Homo sapiens	119-128
10712238-4	2000	Pretreatment of THP-1 cells with PD-98059, an inhibitor of the ERK-kinase cascade, abolished bufalin-induced c-fos and IL-1 beta gene expressions, indicating that the ERK-kinase cascade mediates the induction of inflammatory cytokines by bufalin.	bufalin	93-100	mitogen-activated protein kinase 1	Homo sapiens	167-170
10712238-4	2000	Pretreatment of THP-1 cells with PD-98059, an inhibitor of the ERK-kinase cascade, abolished bufalin-induced c-fos and IL-1 beta gene expressions, indicating that the ERK-kinase cascade mediates the induction of inflammatory cytokines by bufalin.	bufalin	238-245	mitogen-activated protein kinase 1	Homo sapiens	63-66
10712238-4	2000	Pretreatment of THP-1 cells with PD-98059, an inhibitor of the ERK-kinase cascade, abolished bufalin-induced c-fos and IL-1 beta gene expressions, indicating that the ERK-kinase cascade mediates the induction of inflammatory cytokines by bufalin.	bufalin	238-245	mitogen-activated protein kinase 1	Homo sapiens	167-170
10712238-6	2000	These findings suggest that Na(+)-K(+)-ATPase inhibition by bufalin induces calcium influx and thereby activates PKC and ERK.	bufalin	60-67	mitogen-activated protein kinase 1	Homo sapiens	121-124
10712238-7	2000	In cells treated with an inhibitor of p38 MAP kinases, SB-203580, bufalin-mediated ERK activation became persistent and the induction of IL-1 beta and TNF-alpha expressions was significantly augmented.	bufalin	66-73	mitogen-activated protein kinase 14	Homo sapiens	38-41
10712238-7	2000	In cells treated with an inhibitor of p38 MAP kinases, SB-203580, bufalin-mediated ERK activation became persistent and the induction of IL-1 beta and TNF-alpha expressions was significantly augmented.	bufalin	66-73	mitogen-activated protein kinase 1	Homo sapiens	83-86
10229192-5	1999	The increase of Tiam1 mRNA was transient but the level of Tiam1 protein continued to increase at least for 6 h. In addition, the activities of Rac1 and p21-activated kinase (PAK) were also stimulated by bufalin treatment.	bufalin	203-210	TIAM Rac1 associated GEF 1	Homo sapiens	16-21
10229192-5	1999	The increase of Tiam1 mRNA was transient but the level of Tiam1 protein continued to increase at least for 6 h. In addition, the activities of Rac1 and p21-activated kinase (PAK) were also stimulated by bufalin treatment.	bufalin	203-210	TIAM Rac1 associated GEF 1	Homo sapiens	58-63
10229192-5	1999	The increase of Tiam1 mRNA was transient but the level of Tiam1 protein continued to increase at least for 6 h. In addition, the activities of Rac1 and p21-activated kinase (PAK) were also stimulated by bufalin treatment.	bufalin	203-210	Rac family small GTPase 1	Homo sapiens	143-147
10229192-7	1999	Apoptosis was strongly induced by bufalin in cells that expressed sense RNA for Tiam1 as compared to apoptosis in control cells treated with bufalin only.	bufalin	34-41	TIAM Rac1 associated GEF 1	Homo sapiens	80-85
10229192-10	1999	These results suggest that Tiaml might play a critical role in bufalin-induced apoptosis through the activation of Rac1, PAK, and JNK pathway.	bufalin	63-70	Rac family small GTPase 1	Homo sapiens	115-119
10229192-10	1999	These results suggest that Tiaml might play a critical role in bufalin-induced apoptosis through the activation of Rac1, PAK, and JNK pathway.	bufalin	63-70	mitogen-activated protein kinase 8	Homo sapiens	130-133
10423518-5	1999	Moreover, overexpression of bcl-2 in human leukemia HL60 cells that had been transfected with cDNA for bcl-2 prevented bufalin-induced apoptosis but had no significant effect on the change in plasma membrane potential induced by bufalin.	bufalin	119-126	BCL2 apoptosis regulator	Homo sapiens	28-33
10423518-5	1999	Moreover, overexpression of bcl-2 in human leukemia HL60 cells that had been transfected with cDNA for bcl-2 prevented bufalin-induced apoptosis but had no significant effect on the change in plasma membrane potential induced by bufalin.	bufalin	119-126	BCL2 apoptosis regulator	Homo sapiens	103-108
10423518-5	1999	Moreover, overexpression of bcl-2 in human leukemia HL60 cells that had been transfected with cDNA for bcl-2 prevented bufalin-induced apoptosis but had no significant effect on the change in plasma membrane potential induced by bufalin.	bufalin	229-236	BCL2 apoptosis regulator	Homo sapiens	28-33
10423518-6	1999	Since bufalin specifically inhibits the Na+,K(+)-ATPase of human but not murine tumor cells, and since this inhibition leads to a change in intracellular concentration of Na+ ions, our findings suggest that bufalin induces apoptosis in human tumor cells selectively via inhibition of the Na+,K(+)-ATPase, which acts upstream of the bcl-2 protein.	bufalin	207-214	BCL2 apoptosis regulator	Homo sapiens	332-337
10336553-5	1999	Reverse transcription-polymerase chain reaction of additional 14-3-3 isoforms revealed that the eta and gamma isoforms of 14-3-3 are also down-regulated by ouabain, bufalin, and 19-norbufalin derivative, whereas the zeta isoform is down-regulated only by bufalin.	bufalin	165-172	endothelin receptor type A	Rattus norvegicus	96-99
10336553-5	1999	Reverse transcription-polymerase chain reaction of additional 14-3-3 isoforms revealed that the eta and gamma isoforms of 14-3-3 are also down-regulated by ouabain, bufalin, and 19-norbufalin derivative, whereas the zeta isoform is down-regulated only by bufalin.	bufalin	184-191	endothelin receptor type A	Rattus norvegicus	96-99
9781623-3	1998	Recently, we found that administration of bufalin in male rats diminished the luteinizing hormone (LH) response to gonadotropin-releasing hormone (GnRH) and the secretion of testosterone both in vivo and in vitro.	bufalin	42-49	gonadotropin releasing hormone 1	Rattus norvegicus	147-151
9781623-11	1998	These results suggest that the bufalin-free extracts of Chansu inhibit testosterone secretion in rats, in part, due to (1) a decreased production of testicular cAMP, (2) a decreased response of testosterone to gonadotropin, and (3) a reduction of the LH response to GnRH.	bufalin	31-38	gonadotropin releasing hormone 1	Rattus norvegicus	266-270
9458368-5	1998	However, bufalin treatment leads to markedly greater morphologic and functional changes indicative of more extensive differentiation in the cJun overexpressing cells compared to the non-transfected controls.	bufalin	9-16	Jun proto-oncogene, AP-1 transcription factor subunit	Homo sapiens	140-144
9488042-1	1998	In a previous study, we demonstrated that bufalin caused apoptosis in human leukemia U937 cells by the anomalous activation of mitogen-activated protein kinase (MAPK) via a signaling pathway that included Ras, Raf-1 and MAPK kinase-1.	bufalin	42-49	Raf-1 proto-oncogene, serine/threonine kinase	Homo sapiens	210-215
9488042-2	1998	We report here the effect of bufalin on c-Jun N-terminal protein kinase (JNK), a member of the MAPK family, and on the signaling pathway downstream of MAPKs in U937 cells.	bufalin	29-36	mitogen-activated protein kinase 8	Homo sapiens	40-71
9488042-2	1998	We report here the effect of bufalin on c-Jun N-terminal protein kinase (JNK), a member of the MAPK family, and on the signaling pathway downstream of MAPKs in U937 cells.	bufalin	29-36	mitogen-activated protein kinase 8	Homo sapiens	73-76
9488042-3	1998	When U937 cells were treated with 10(-8) M bufalin, the activity of JNK1 was markedly elevated 3 h after the start of treatment and remained so for 9 h. This activation of JNK and the induction of apoptosis by bufalin were suppressed by expression of antisense mRNA for MAPK kinase-1.	bufalin	43-50	mitogen-activated protein kinase 8	Homo sapiens	68-72
9488042-3	1998	When U937 cells were treated with 10(-8) M bufalin, the activity of JNK1 was markedly elevated 3 h after the start of treatment and remained so for 9 h. This activation of JNK and the induction of apoptosis by bufalin were suppressed by expression of antisense mRNA for MAPK kinase-1.	bufalin	43-50	mitogen-activated protein kinase 8	Homo sapiens	68-71
9488042-3	1998	When U937 cells were treated with 10(-8) M bufalin, the activity of JNK1 was markedly elevated 3 h after the start of treatment and remained so for 9 h. This activation of JNK and the induction of apoptosis by bufalin were suppressed by expression of antisense mRNA for MAPK kinase-1.	bufalin	210-217	mitogen-activated protein kinase 8	Homo sapiens	68-72
9488042-3	1998	When U937 cells were treated with 10(-8) M bufalin, the activity of JNK1 was markedly elevated 3 h after the start of treatment and remained so for 9 h. This activation of JNK and the induction of apoptosis by bufalin were suppressed by expression of antisense mRNA for MAPK kinase-1.	bufalin	210-217	mitogen-activated protein kinase 8	Homo sapiens	68-71
9488042-4	1998	c-Jun was translocated from the cytoplasm to the nucleus after treatment of U937 cells with bufalin.	bufalin	92-99	Jun proto-oncogene, AP-1 transcription factor subunit	Homo sapiens	0-5
9488042-8	1998	These results suggest that the activation of AP-1 via a MAPK cascade that includes JNK is required for the induction of apoptosis by bufalin in U937 cells.	bufalin	133-140	mitogen-activated protein kinase 8	Homo sapiens	83-86
9458368-6	1998	Furthermore, cJun overexpressing cells maintain greater viability in the presence of bufalin and arrest in a different phase of the cell cycle than do control cell lines (G0/G1 versus S/G2M, respectively), although some apoptosis occurs in all cell lines treated with bufalin.	bufalin	85-92	Jun proto-oncogene, AP-1 transcription factor subunit	Homo sapiens	13-17
9458368-6	1998	Furthermore, cJun overexpressing cells maintain greater viability in the presence of bufalin and arrest in a different phase of the cell cycle than do control cell lines (G0/G1 versus S/G2M, respectively), although some apoptosis occurs in all cell lines treated with bufalin.	bufalin	268-275	Jun proto-oncogene, AP-1 transcription factor subunit	Homo sapiens	13-17
9353366-9	1997	Administration of bufalin in vitro resulted in an inhibition of both basal and hCG-stimulated release of testosterone.	bufalin	18-25	chorionic gonadotropin subunit beta 5	Homo sapiens	79-82
9353366-12	1997	Likewise, the LH response to GnRH was diminished by bufalin administration, both in vivo and in vitro.	bufalin	52-59	gonadotropin releasing hormone 1	Rattus norvegicus	29-33
9353366-13	1997	These results suggest that the inhibition of testosterone production by bufalin is partly caused by a decrease of testicular cAMP accumulation and LH response to GnRH in rats.	bufalin	72-79	gonadotropin releasing hormone 1	Rattus norvegicus	162-166
8662906-2	1996	When U937 cells were treated with 10(-8) M bufalin in the absence of serum, mitogen-activated protein (MAP) kinase activity was markedly increased 6 h after the start of treatment and elevated so for 12 h. Prior to the activation of MAP kinase, increased activities of Ras, Raf-1, and MAP kinase kinase were found, but these enzymes were transiently activated by the treatment with bufalin.	bufalin	43-50	Raf-1 proto-oncogene, serine/threonine kinase	Homo sapiens	274-279
9242431-0	1997	Bcl-2 protein inhibits bufalin-induced apoptosis through inhibition of mitogen-activated protein kinase activation in human leukemia U937 cells.	bufalin	23-30	BCL2 apoptosis regulator	Homo sapiens	0-5
9242431-2	1997	Here, we report the effect of overexpression of bcl-2 in U937 cells on the signaling pathway of apoptosis that is induced by bufalin.	bufalin	125-132	BCL2 apoptosis regulator	Homo sapiens	48-53
9242431-4	1997	No significant difference was detected in the activation of MAPK kinase-1 that is induced by bufalin in wild-type or Bcl-2-overexpressed U937 cells; however, the activation of MAPK by bufalin was significantly attenuated in the cells overexpressing Bcl-2.	bufalin	184-191	BCL2 apoptosis regulator	Homo sapiens	249-254
9242431-5	1997	Bufalin treatment activated activator protein-1 transcriptional activity; however, this activation was decreased to 40% in bcl-2-overexpressed U937 cells.	bufalin	0-7	FosB proto-oncogene, AP-1 transcription factor subunit	Homo sapiens	28-47
9242431-5	1997	Bufalin treatment activated activator protein-1 transcriptional activity; however, this activation was decreased to 40% in bcl-2-overexpressed U937 cells.	bufalin	0-7	BCL2 apoptosis regulator	Homo sapiens	123-128
8662906-5	1996	In fact, pretreatment of U937 cells with forskolin and 3-isobutyl-1-methylxanthine, which are known to increase the concentration of cAMP in the cells, and subsequent treatment with bufalin resulted in a decrease in both Raf-1 activity and DNA fragmentation.	bufalin	182-189	Raf-1 proto-oncogene, serine/threonine kinase	Homo sapiens	221-226
7658701-2	1995	In the present study, we found that bufalin at concentrations of 10(-7) M and higher induced apoptosis in human leukemia cells, such as HL60, ML1, but not in mouse leukemia M1 cells.	bufalin	36-43	interleukin 17F	Homo sapiens	142-145
7658701-5	1995	Northern blot analysis revealed that the levels of expression of the c-myc and bcl-2 genes in HL60 cells decreased with time after treatment with bufalin.	bufalin	146-153	MYC proto-oncogene, bHLH transcription factor	Homo sapiens	69-74
7658701-5	1995	Northern blot analysis revealed that the levels of expression of the c-myc and bcl-2 genes in HL60 cells decreased with time after treatment with bufalin.	bufalin	146-153	BCL2 apoptosis regulator	Homo sapiens	79-84
8063619-3	1994	HL-60, ML1, and U937 leukemia cells treated with bufalin at 10(-8) M and above had condensed and fragmented nuclei.	bufalin	49-56	interleukin 17F	Homo sapiens	7-10
1324788-2	1992	The present work was carried out to examine further the effect of bufalin on the growth and characteristics of human leukemia-derived cell lines U937, ML1, and HL60.	bufalin	66-73	interleukin 17F	Homo sapiens	151-154
8074471-4	1994	Activities of PKC, PKA, cdc2 kinase and CK II in ML1 cells were changed early by bufalin; PKA and PKC activities were inhibited, and cdc2 kinase and CK II activities were increased.	bufalin	81-88	casein kinase 2 alpha 1	Homo sapiens	40-45
1324788-3	1992	At concentrations of 5-10 nM, bufalin decreased the growth of ML1 cells preferentially at the G2 phase and U937 cells at the S and G2 phases of the cell cycle.	bufalin	30-37	interleukin 17F	Homo sapiens	62-65
1324788-4	1992	Bufalin, under these conditions, induced the differentiation of U937, ML1, and HL60 cells to monocyte/macrophage-like cells by measuring the expression of various differentiation markers, as assessed by morphology and histochemistry, and ability to phagocytose latex particles, to reduce nitroblue tetrazolium, and to develop Fc receptors.	bufalin	0-7	interleukin 17F	Homo sapiens	70-73
1324788-5	1992	U937 and ML1 cells started to differentiate at 4 and 6 h, respectively, after treatment with 10 nM bufalin and showed maximum differentiation 72 h later.	bufalin	99-106	interleukin 17F	Homo sapiens	9-12
34512368-3	2021	In this review, we focus on summarizing some representative natural active compounds, mainly including curcumin, resveratrol, paclitaxel, Bufalin, and Ursolic acid that may ultimately trigger cancer cell death through the regulation of some key autophagic signaling pathways, such as RAS-RAF-MEK-ERK, PI3K-AKT-mTOR, AMPK, ULK1, Beclin-1, Atg5 and p53.	bufalin	138-145	mitogen-activated protein kinase kinase 7	Homo sapiens	292-295
1614270-2	1992	The effect of bufalin on melanogenesis included stimulation of tyrosinase activity and increase of cellular melanin content.	bufalin	14-21	tyrosinase	Mus musculus	63-73
1859424-2	1991	Bufalin, at a concentration as low as 10 nM, also produced a strong differentiation-inducing activity in three other human leukemia-derived cell lines (human promyelocytic HL60, monoblastic U937 and myeloblastic ML1).	bufalin	0-7	interleukin 17F	Homo sapiens	212-215
34678691-0	2021	Bufalin enhances the killing efficacy of NK cells against hepatocellular carcinoma by inhibiting MICA shedding.	bufalin	0-7	MHC class I polypeptide-related sequence A	Homo sapiens	97-101
34678691-3	2021	We found that bufalin directly balances stimulatory and inhibitory receptors on the surface of NK cells and indirectly activates natural killer (NK) cells by inhibiting MICA shedding, which prevented immune escape and indirectly enhanced NKG2D-dependent immune surveillance.	bufalin	14-21	MHC class I polypeptide-related sequence A	Homo sapiens	169-173
34678691-3	2021	We found that bufalin directly balances stimulatory and inhibitory receptors on the surface of NK cells and indirectly activates natural killer (NK) cells by inhibiting MICA shedding, which prevented immune escape and indirectly enhanced NKG2D-dependent immune surveillance.	bufalin	14-21	killer cell lectin like receptor K1	Homo sapiens	238-243
34826043-0	2021	Bufalin Induces Programmed Necroptosis in Triple-Negative Breast Cancer Drug-Resistant Cell Lines through RIP1/ROS-Mediated Pathway.	bufalin	0-7	receptor interacting serine/threonine kinase 1	Homo sapiens	106-110
34826043-7	2021	After treatment with bufalin, the expressions of tumor necrosis factor alpha, phospho-tumor necrosis factor receptor 1, phospho-receptor interacting protein 1 and c-caspase 3 increased (all P<0.01), the killing effect of bufalin could be mostly inhibited by NEC-1, and by z-VAD-fmk (both P<0.01).	bufalin	21-28	tumor necrosis factor	Homo sapiens	49-76
34826043-7	2021	After treatment with bufalin, the expressions of tumor necrosis factor alpha, phospho-tumor necrosis factor receptor 1, phospho-receptor interacting protein 1 and c-caspase 3 increased (all P<0.01), the killing effect of bufalin could be mostly inhibited by NEC-1, and by z-VAD-fmk (both P<0.01).	bufalin	21-28	receptor interacting serine/threonine kinase 1	Homo sapiens	128-158
34826043-7	2021	After treatment with bufalin, the expressions of tumor necrosis factor alpha, phospho-tumor necrosis factor receptor 1, phospho-receptor interacting protein 1 and c-caspase 3 increased (all P<0.01), the killing effect of bufalin could be mostly inhibited by NEC-1, and by z-VAD-fmk (both P<0.01).	bufalin	21-28	proprotein convertase subtilisin/kexin type 1	Homo sapiens	258-263
34826043-7	2021	After treatment with bufalin, the expressions of tumor necrosis factor alpha, phospho-tumor necrosis factor receptor 1, phospho-receptor interacting protein 1 and c-caspase 3 increased (all P<0.01), the killing effect of bufalin could be mostly inhibited by NEC-1, and by z-VAD-fmk (both P<0.01).	bufalin	221-228	tumor necrosis factor	Homo sapiens	49-76
34826043-7	2021	After treatment with bufalin, the expressions of tumor necrosis factor alpha, phospho-tumor necrosis factor receptor 1, phospho-receptor interacting protein 1 and c-caspase 3 increased (all P<0.01), the killing effect of bufalin could be mostly inhibited by NEC-1, and by z-VAD-fmk (both P<0.01).	bufalin	221-228	receptor interacting serine/threonine kinase 1	Homo sapiens	128-158
34826043-7	2021	After treatment with bufalin, the expressions of tumor necrosis factor alpha, phospho-tumor necrosis factor receptor 1, phospho-receptor interacting protein 1 and c-caspase 3 increased (all P<0.01), the killing effect of bufalin could be mostly inhibited by NEC-1, and by z-VAD-fmk (both P<0.01).	bufalin	221-228	proprotein convertase subtilisin/kexin type 1	Homo sapiens	258-263
34826043-8	2021	Besides, the intracellular reactive oxygen species (ROS) levels increased considerably (P<0.01), the antioxidant N-acetyl cysteine or Nec-1 could inhibit the increase of ROS level and the killing effect of bufalin (all P<0.01).	bufalin	206-213	proprotein convertase subtilisin/kexin type 1	Homo sapiens	134-139
34826043-10	2021	CONCLUSIONS: Bufalin could induce necroptosis through RIP1/ROS-mediated pathway to kill the drug-resistant TNBC cell lines.	bufalin	13-20	receptor interacting serine/threonine kinase 1	Homo sapiens	54-58
34481023-0	2021	Co-delivery of bufalin and nintedanib via albumin sub-microspheres for synergistic cancer therapy.	bufalin	15-22	albumin	Homo sapiens	42-49
34373701-10	2021	The combination of bufalin and hydroxycampothecin also increased the expression of apoptosis-related proteins Bax, p53, PDCD4 and GSK-3beta, and decreased the expression of Bcl-XL and p-AKT compared with a single drug treatment.	bufalin	19-26	BCL2-associated X protein	Mus musculus	110-113
34373701-10	2021	The combination of bufalin and hydroxycampothecin also increased the expression of apoptosis-related proteins Bax, p53, PDCD4 and GSK-3beta, and decreased the expression of Bcl-XL and p-AKT compared with a single drug treatment.	bufalin	19-26	transformation related protein 53, pseudogene	Mus musculus	115-118
34373701-10	2021	The combination of bufalin and hydroxycampothecin also increased the expression of apoptosis-related proteins Bax, p53, PDCD4 and GSK-3beta, and decreased the expression of Bcl-XL and p-AKT compared with a single drug treatment.	bufalin	19-26	programmed cell death 4	Mus musculus	120-125
34373701-10	2021	The combination of bufalin and hydroxycampothecin also increased the expression of apoptosis-related proteins Bax, p53, PDCD4 and GSK-3beta, and decreased the expression of Bcl-XL and p-AKT compared with a single drug treatment.	bufalin	19-26	glycogen synthase kinase 3 alpha	Mus musculus	130-139
34373701-10	2021	The combination of bufalin and hydroxycampothecin also increased the expression of apoptosis-related proteins Bax, p53, PDCD4 and GSK-3beta, and decreased the expression of Bcl-XL and p-AKT compared with a single drug treatment.	bufalin	19-26	BCL2-like 1	Mus musculus	173-179
34373701-10	2021	The combination of bufalin and hydroxycampothecin also increased the expression of apoptosis-related proteins Bax, p53, PDCD4 and GSK-3beta, and decreased the expression of Bcl-XL and p-AKT compared with a single drug treatment.	bufalin	19-26	thymoma viral proto-oncogene 1	Mus musculus	186-189
34373701-11	2021	The present study suggested that the combination of bufalin and hydroxycampothecin improved the inhibitory effects of both drugs on CRPC tumors in vivo, potentially via the regulation of the PI3K/AKT/GSK-3beta and p53-dependent apoptosis signaling pathways.	bufalin	52-59	thymoma viral proto-oncogene 1	Mus musculus	196-199
34373701-11	2021	The present study suggested that the combination of bufalin and hydroxycampothecin improved the inhibitory effects of both drugs on CRPC tumors in vivo, potentially via the regulation of the PI3K/AKT/GSK-3beta and p53-dependent apoptosis signaling pathways.	bufalin	52-59	glycogen synthase kinase 3 alpha	Mus musculus	200-209
34373701-11	2021	The present study suggested that the combination of bufalin and hydroxycampothecin improved the inhibitory effects of both drugs on CRPC tumors in vivo, potentially via the regulation of the PI3K/AKT/GSK-3beta and p53-dependent apoptosis signaling pathways.	bufalin	52-59	transformation related protein 53, pseudogene	Mus musculus	214-217
34935692-7	2021	Bufalin was confirmed to combine with EGFR protein using molecular docking and downregulated expression of EGFR.	bufalin	0-7	epidermal growth factor receptor	Homo sapiens	38-42
34935692-7	2021	Bufalin was confirmed to combine with EGFR protein using molecular docking and downregulated expression of EGFR.	bufalin	0-7	epidermal growth factor receptor	Homo sapiens	107-111
34935692-8	2021	Bufalin inhibited phosphorylation of EGFR, protein kinase B (AKT), and extracellular signal-regulated kinase (ERK).	bufalin	0-7	epidermal growth factor receptor	Homo sapiens	37-41
34935692-8	2021	Bufalin inhibited phosphorylation of EGFR, protein kinase B (AKT), and extracellular signal-regulated kinase (ERK).	bufalin	0-7	protein tyrosine kinase 2 beta	Homo sapiens	43-59
34935692-8	2021	Bufalin inhibited phosphorylation of EGFR, protein kinase B (AKT), and extracellular signal-regulated kinase (ERK).	bufalin	0-7	AKT serine/threonine kinase 1	Homo sapiens	61-64
34935692-8	2021	Bufalin inhibited phosphorylation of EGFR, protein kinase B (AKT), and extracellular signal-regulated kinase (ERK).	bufalin	0-7	mitogen-activated protein kinase 1	Homo sapiens	71-108
34935692-8	2021	Bufalin inhibited phosphorylation of EGFR, protein kinase B (AKT), and extracellular signal-regulated kinase (ERK).	bufalin	0-7	mitogen-activated protein kinase 1	Homo sapiens	110-113
34935692-9	2021	CONCLUSION: Bufalin suppresses the proliferation of ovarian cancer cells through the EGFR/AKT/ERK signaling pathway.	bufalin	12-19	epidermal growth factor receptor	Homo sapiens	85-89
34935692-9	2021	CONCLUSION: Bufalin suppresses the proliferation of ovarian cancer cells through the EGFR/AKT/ERK signaling pathway.	bufalin	12-19	AKT serine/threonine kinase 1	Homo sapiens	90-93
34935692-9	2021	CONCLUSION: Bufalin suppresses the proliferation of ovarian cancer cells through the EGFR/AKT/ERK signaling pathway.	bufalin	12-19	mitogen-activated protein kinase 1	Homo sapiens	94-97
34481023-4	2021	Herein, we developed multifunctional albumin sub-microspheres prepared by coaxial-electrospray technology to co-delivery bufalin and nintedanib for tumor-targeted combination therapy.	bufalin	121-128	albumin	Homo sapiens	37-44
34583803-10	2021	Noteworthy, anti-cancer compounds including bufalin and ovatodiolide are suggested to regulate miRNA/SOX2 axis in cancers.	bufalin	44-51	SRY-box transcription factor 2	Homo sapiens	101-105
34496870-0	2021	Bufalin suppresses tumour microenvironment-mediated angiogenesis by inhibiting the STAT3 signalling pathway.	bufalin	0-7	signal transducer and activator of transcription 3	Homo sapiens	83-88
34496870-9	2021	The results demonstrated that the proangiogenic genes in HUVECs, such as VEGF, PDGFA, E-selectin and P-selectin, were downregulated by bufalin and that this downregulation was mediated by inhibition of the STAT3 pathway.	bufalin	135-142	vascular endothelial growth factor A	Homo sapiens	73-77
34496870-9	2021	The results demonstrated that the proangiogenic genes in HUVECs, such as VEGF, PDGFA, E-selectin and P-selectin, were downregulated by bufalin and that this downregulation was mediated by inhibition of the STAT3 pathway.	bufalin	135-142	platelet derived growth factor subunit A	Homo sapiens	79-84
34496870-9	2021	The results demonstrated that the proangiogenic genes in HUVECs, such as VEGF, PDGFA, E-selectin and P-selectin, were downregulated by bufalin and that this downregulation was mediated by inhibition of the STAT3 pathway.	bufalin	135-142	selectin E	Homo sapiens	86-96
34496870-9	2021	The results demonstrated that the proangiogenic genes in HUVECs, such as VEGF, PDGFA, E-selectin and P-selectin, were downregulated by bufalin and that this downregulation was mediated by inhibition of the STAT3 pathway.	bufalin	135-142	selectin P	Homo sapiens	101-111
34496870-9	2021	The results demonstrated that the proangiogenic genes in HUVECs, such as VEGF, PDGFA, E-selectin and P-selectin, were downregulated by bufalin and that this downregulation was mediated by inhibition of the STAT3 pathway.	bufalin	135-142	signal transducer and activator of transcription 3	Homo sapiens	206-211
34496870-10	2021	Overexpression of STAT3 reversed the inhibitory effects of bufalin on angiogenesis.	bufalin	59-66	signal transducer and activator of transcription 3	Homo sapiens	18-23
34496870-12	2021	CONCLUSION: Our findings demonstrate that bufalin suppresses tumour microenvironment-mediated angiogenesis by inhibiting the STAT3 signalling pathway in vascular endothelial cells, revealing that bufalin may be used as a new antiangiogenic adjuvant therapy medicine to treat colorectal cancer.	bufalin	42-49	signal transducer and activator of transcription 3	Homo sapiens	125-130
34512368-3	2021	In this review, we focus on summarizing some representative natural active compounds, mainly including curcumin, resveratrol, paclitaxel, Bufalin, and Ursolic acid that may ultimately trigger cancer cell death through the regulation of some key autophagic signaling pathways, such as RAS-RAF-MEK-ERK, PI3K-AKT-mTOR, AMPK, ULK1, Beclin-1, Atg5 and p53.	bufalin	138-145	mitogen-activated protein kinase 1	Homo sapiens	296-299
34512368-3	2021	In this review, we focus on summarizing some representative natural active compounds, mainly including curcumin, resveratrol, paclitaxel, Bufalin, and Ursolic acid that may ultimately trigger cancer cell death through the regulation of some key autophagic signaling pathways, such as RAS-RAF-MEK-ERK, PI3K-AKT-mTOR, AMPK, ULK1, Beclin-1, Atg5 and p53.	bufalin	138-145	AKT serine/threonine kinase 1	Homo sapiens	306-309
34512368-3	2021	In this review, we focus on summarizing some representative natural active compounds, mainly including curcumin, resveratrol, paclitaxel, Bufalin, and Ursolic acid that may ultimately trigger cancer cell death through the regulation of some key autophagic signaling pathways, such as RAS-RAF-MEK-ERK, PI3K-AKT-mTOR, AMPK, ULK1, Beclin-1, Atg5 and p53.	bufalin	138-145	mechanistic target of rapamycin kinase	Homo sapiens	310-314
34512368-3	2021	In this review, we focus on summarizing some representative natural active compounds, mainly including curcumin, resveratrol, paclitaxel, Bufalin, and Ursolic acid that may ultimately trigger cancer cell death through the regulation of some key autophagic signaling pathways, such as RAS-RAF-MEK-ERK, PI3K-AKT-mTOR, AMPK, ULK1, Beclin-1, Atg5 and p53.	bufalin	138-145	beclin 1	Homo sapiens	328-336
34512368-3	2021	In this review, we focus on summarizing some representative natural active compounds, mainly including curcumin, resveratrol, paclitaxel, Bufalin, and Ursolic acid that may ultimately trigger cancer cell death through the regulation of some key autophagic signaling pathways, such as RAS-RAF-MEK-ERK, PI3K-AKT-mTOR, AMPK, ULK1, Beclin-1, Atg5 and p53.	bufalin	138-145	autophagy related 5	Homo sapiens	338-342
35572840-0	2022	Alisol B 23-Acetate Increases the Antitumor Effect of Bufalin on Liver Cancer through Inactivating Wnt/beta-Catenin Axis.	bufalin	54-61	catenin beta 1	Homo sapiens	103-115
34376212-0	2021	Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression.	bufalin	0-7	annexin A2	Homo sapiens	95-105
34376212-0	2021	Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression.	bufalin	0-7	utrophin	Homo sapiens	110-114
34376212-8	2021	siRNA targeting Annexin A2 and the DRP1 protein inhibitor Mdivi-1 were used to confirm the targets of bufalin.	bufalin	102-109	annexin A2	Homo sapiens	16-26
34376212-8	2021	siRNA targeting Annexin A2 and the DRP1 protein inhibitor Mdivi-1 were used to confirm the targets of bufalin.	bufalin	102-109	utrophin	Homo sapiens	35-39
34376212-9	2021	RESULTS: Bufalin upregulated the expression of cytochrome C, cleaved caspase 3, p-Chk1 and p-p53 proteins to induce U251 cell apoptosis and cycle arrest in the S phase.	bufalin	9-16	cytochrome c, somatic	Homo sapiens	47-59
34376212-9	2021	RESULTS: Bufalin upregulated the expression of cytochrome C, cleaved caspase 3, p-Chk1 and p-p53 proteins to induce U251 cell apoptosis and cycle arrest in the S phase.	bufalin	9-16	caspase 3	Homo sapiens	69-78
34376212-9	2021	RESULTS: Bufalin upregulated the expression of cytochrome C, cleaved caspase 3, p-Chk1 and p-p53 proteins to induce U251 cell apoptosis and cycle arrest in the S phase.	bufalin	9-16	checkpoint kinase 1	Homo sapiens	82-86
34376212-9	2021	RESULTS: Bufalin upregulated the expression of cytochrome C, cleaved caspase 3, p-Chk1 and p-p53 proteins to induce U251 cell apoptosis and cycle arrest in the S phase.	bufalin	9-16	tumor protein p53	Homo sapiens	93-96
34376212-11	2021	The expression of mitochondrial division-/fusion-related proteins in U251 cells was abnormal, the Annexin A2 and DRP1 proteins were translocated from the cytoplasm to mitochondria, and the MFN2 protein was released from mitochondria into the cytoplasm after bufalin treatment, disrupting the mitochondrial division/fusion balance in U251 cells.	bufalin	258-265	mitofusin 2	Homo sapiens	189-193
34376212-12	2021	CONCLUSIONS: Our research indicated that bufalin can cause Annexin A2 and DRP1 oligomerization on the surface of mitochondria and disrupt the mitochondrial division/fusion balance to induce U251 cell apoptosis.	bufalin	41-48	annexin A2	Homo sapiens	59-69
34376212-12	2021	CONCLUSIONS: Our research indicated that bufalin can cause Annexin A2 and DRP1 oligomerization on the surface of mitochondria and disrupt the mitochondrial division/fusion balance to induce U251 cell apoptosis.	bufalin	41-48	utrophin	Homo sapiens	74-78
35572840-11	2022	In addition, alisol B 23-acetate notably enhanced the apoptotic effect of bufalin on liver cancer cells through mediation of Mcl-1, Bax, Bcl-2, and cleaved caspase-3.	bufalin	74-81	MCL1 apoptosis regulator, BCL2 family member	Homo sapiens	125-130
35572840-11	2022	In addition, alisol B 23-acetate notably enhanced the apoptotic effect of bufalin on liver cancer cells through mediation of Mcl-1, Bax, Bcl-2, and cleaved caspase-3.	bufalin	74-81	BCL2 associated X, apoptosis regulator	Homo sapiens	132-135
35572840-11	2022	In addition, alisol B 23-acetate notably enhanced the apoptotic effect of bufalin on liver cancer cells through mediation of Mcl-1, Bax, Bcl-2, and cleaved caspase-3.	bufalin	74-81	BCL2 apoptosis regulator	Homo sapiens	137-142
35572840-11	2022	In addition, alisol B 23-acetate notably enhanced the apoptotic effect of bufalin on liver cancer cells through mediation of Mcl-1, Bax, Bcl-2, and cleaved caspase-3.	bufalin	74-81	caspase 3	Homo sapiens	156-165
35572840-12	2022	Meanwhile, alisol B 23-acetate in combination with bufalin induced the autophagy in liver cancer cells through mediation of Beclin-1 and p62.	bufalin	51-58	beclin 1	Homo sapiens	124-132
35572840-12	2022	Meanwhile, alisol B 23-acetate in combination with bufalin induced the autophagy in liver cancer cells through mediation of Beclin-1 and p62.	bufalin	51-58	nucleoporin 62	Homo sapiens	137-140
35572840-13	2022	Furthermore, alisol B 23-acetate in combination with bufalin significantly downregulated the level of GSK-3beta and increased the expression of beta-catenin in liver cancer cells.	bufalin	53-60	glycogen synthase kinase 3 alpha	Homo sapiens	102-111
35572840-13	2022	Furthermore, alisol B 23-acetate in combination with bufalin significantly downregulated the level of GSK-3beta and increased the expression of beta-catenin in liver cancer cells.	bufalin	53-60	catenin beta 1	Homo sapiens	144-156
35572840-14	2022	Conclusion: In summary, these findings provide the first evidence that alisol B 23-acetate improves the anticancer activity of bufalin on liver cancer through activation of the Wnt/beta-catenin axis, and these outcomes might shed new lights on exploring the new methods against liver cancer.	bufalin	127-134	catenin beta 1	Homo sapiens	181-193
35618286-9	2022	Mechanistically, bufalin inhibits overexpression of p50 nuclear factor kappa B (NF-kappaB) factor, leading to the predominance of p65-p50 heterodimers over p50 homodimers in the nuclei.	bufalin	17-24	nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105	Mus musculus	52-55
35618286-9	2022	Mechanistically, bufalin inhibits overexpression of p50 nuclear factor kappa B (NF-kappaB) factor, leading to the predominance of p65-p50 heterodimers over p50 homodimers in the nuclei.	bufalin	17-24	v-rel reticuloendotheliosis viral oncogene homolog A (avian)	Mus musculus	130-133
35618286-9	2022	Mechanistically, bufalin inhibits overexpression of p50 nuclear factor kappa B (NF-kappaB) factor, leading to the predominance of p65-p50 heterodimers over p50 homodimers in the nuclei.	bufalin	17-24	nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105	Mus musculus	134-137
35618286-9	2022	Mechanistically, bufalin inhibits overexpression of p50 nuclear factor kappa B (NF-kappaB) factor, leading to the predominance of p65-p50 heterodimers over p50 homodimers in the nuclei.	bufalin	17-24	nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105	Mus musculus	156-159
35126601-4	2022	Furthermore, in vitro cell experiments were used to verify bufalin activation of AKT and MAPK signaling pathways in human mesangial cells.	bufalin	59-66	AKT serine/threonine kinase 1	Homo sapiens	81-84
35094507-2	2022	Here, we report that a natural compound bufalin (BF) could time-dependently inactivate hCYP3A via complex CYP-catalyzed cascade oxidative metabolism.	bufalin	40-47	cytochrome P450 family 3 subfamily A member 4	Homo sapiens	87-93
35094507-2	2022	Here, we report that a natural compound bufalin (BF) could time-dependently inactivate hCYP3A via complex CYP-catalyzed cascade oxidative metabolism.	bufalin	40-47	peptidylprolyl isomerase G	Homo sapiens	106-109
35094507-2	2022	Here, we report that a natural compound bufalin (BF) could time-dependently inactivate hCYP3A via complex CYP-catalyzed cascade oxidative metabolism.	bufalin	49-51	cytochrome P450 family 3 subfamily A member 4	Homo sapiens	87-93
35094507-2	2022	Here, we report that a natural compound bufalin (BF) could time-dependently inactivate hCYP3A via complex CYP-catalyzed cascade oxidative metabolism.	bufalin	49-51	peptidylprolyl isomerase G	Homo sapiens	106-109
35241510-11	2022	Compared to sorafenib or bufalin treatment alone, the combination treatment resulted in lower Bcl-2 expression but higher Bax, Bad, APAF-1, caspase-3, and caspase-9.	bufalin	25-32	BCL2 apoptosis regulator	Homo sapiens	94-99
35241510-11	2022	Compared to sorafenib or bufalin treatment alone, the combination treatment resulted in lower Bcl-2 expression but higher Bax, Bad, APAF-1, caspase-3, and caspase-9.	bufalin	25-32	BCL2 associated X, apoptosis regulator	Homo sapiens	122-125
35241510-11	2022	Compared to sorafenib or bufalin treatment alone, the combination treatment resulted in lower Bcl-2 expression but higher Bax, Bad, APAF-1, caspase-3, and caspase-9.	bufalin	25-32	apoptotic peptidase activating factor 1	Homo sapiens	132-138
35241510-11	2022	Compared to sorafenib or bufalin treatment alone, the combination treatment resulted in lower Bcl-2 expression but higher Bax, Bad, APAF-1, caspase-3, and caspase-9.	bufalin	25-32	caspase 3	Homo sapiens	140-149
35241510-11	2022	Compared to sorafenib or bufalin treatment alone, the combination treatment resulted in lower Bcl-2 expression but higher Bax, Bad, APAF-1, caspase-3, and caspase-9.	bufalin	25-32	caspase 9	Homo sapiens	155-164
35409366-1	2022	Bufalin and other cardiac steroids (CS) have been used for centuries for the treatment of congestive heart failure, arrhythmias, and other maladies.	bufalin	0-7	citrate synthase	Homo sapiens	36-38
33913201-0	2021	Bufalin inhibits peritoneal dissemination of gastric cancer through endothelial nitric oxide synthase-mitogen-activated protein kinases signaling pathway.	bufalin	0-7	nitric oxide synthase 3	Homo sapiens	68-101
33990545-2	2021	Here, SDC4 was identified as a direct cellular target of small-molecule bufalin with anti-hepatocellular carcinoma (HCC) activity.	bufalin	72-79	syndecan 4	Homo sapiens	6-10
33990545-3	2021	Mechanism studies revealed that bufalin directly bond to SDC4 and selectively increased SDC4 interaction with substrate protein DEAD-box helicase 23 (DDX23) to induce HCC genomic instability.	bufalin	32-39	syndecan 4	Homo sapiens	57-61
33990545-3	2021	Mechanism studies revealed that bufalin directly bond to SDC4 and selectively increased SDC4 interaction with substrate protein DEAD-box helicase 23 (DDX23) to induce HCC genomic instability.	bufalin	32-39	syndecan 4	Homo sapiens	88-92
33990545-3	2021	Mechanism studies revealed that bufalin directly bond to SDC4 and selectively increased SDC4 interaction with substrate protein DEAD-box helicase 23 (DDX23) to induce HCC genomic instability.	bufalin	32-39	DEAD-box helicase 23	Homo sapiens	128-148
33990545-3	2021	Mechanism studies revealed that bufalin directly bond to SDC4 and selectively increased SDC4 interaction with substrate protein DEAD-box helicase 23 (DDX23) to induce HCC genomic instability.	bufalin	32-39	DEAD-box helicase 23	Homo sapiens	150-155
33990545-5	2021	Notably, specific knockdown of SDC4 or DDX23 markedly abolished bufalin-dependent inhibition of HCC proliferation and migration, indicating SDC4/DDX23 signaling axis is highly involved in the HCC process.	bufalin	64-71	syndecan 4	Homo sapiens	31-35
33990545-5	2021	Notably, specific knockdown of SDC4 or DDX23 markedly abolished bufalin-dependent inhibition of HCC proliferation and migration, indicating SDC4/DDX23 signaling axis is highly involved in the HCC process.	bufalin	64-71	DEAD-box helicase 23	Homo sapiens	39-44
33990545-5	2021	Notably, specific knockdown of SDC4 or DDX23 markedly abolished bufalin-dependent inhibition of HCC proliferation and migration, indicating SDC4/DDX23 signaling axis is highly involved in the HCC process.	bufalin	64-71	syndecan 4	Homo sapiens	140-144
33990545-5	2021	Notably, specific knockdown of SDC4 or DDX23 markedly abolished bufalin-dependent inhibition of HCC proliferation and migration, indicating SDC4/DDX23 signaling axis is highly involved in the HCC process.	bufalin	64-71	DEAD-box helicase 23	Homo sapiens	145-150
34991674-3	2022	We used Bufalin as a probe to screen its potential targets by proteome microarray, in which AHSA1 was the unique target of Bufalin.	bufalin	8-15	activator of HSP90 ATPase activity 1	Homo sapiens	92-97
34991674-3	2022	We used Bufalin as a probe to screen its potential targets by proteome microarray, in which AHSA1 was the unique target of Bufalin.	bufalin	123-130	activator of HSP90 ATPase activity 1	Homo sapiens	92-97
34991674-5	2022	The potential mechanisms of Bufalin and KU-177 in AHSA1/HSP90 were verified by co-immunoprecipitation, mass spectrometry, site mutation and microscale thermophoresis assay.	bufalin	28-35	activator of HSP90 ATPase activity 1	Homo sapiens	50-55
34991674-5	2022	The potential mechanisms of Bufalin and KU-177 in AHSA1/HSP90 were verified by co-immunoprecipitation, mass spectrometry, site mutation and microscale thermophoresis assay.	bufalin	28-35	heat shock protein 90 alpha family class A member 1	Homo sapiens	56-61
34991674-9	2022	Additionally, we identified AHSA1-K137 as the specific binding site of Bufalin on AHSA1, mutation of which decreased the interaction of AHSA1 with HSP90A and suppressed the function of AHSA1 on mediating CDK6 and PSMD2.	bufalin	71-78	activator of HSP90 ATPase activity 1	Homo sapiens	28-33
34991674-9	2022	Additionally, we identified AHSA1-K137 as the specific binding site of Bufalin on AHSA1, mutation of which decreased the interaction of AHSA1 with HSP90A and suppressed the function of AHSA1 on mediating CDK6 and PSMD2.	bufalin	71-78	activator of HSP90 ATPase activity 1	Homo sapiens	82-87
34991674-9	2022	Additionally, we identified AHSA1-K137 as the specific binding site of Bufalin on AHSA1, mutation of which decreased the interaction of AHSA1 with HSP90A and suppressed the function of AHSA1 on mediating CDK6 and PSMD2.	bufalin	71-78	activator of HSP90 ATPase activity 1	Homo sapiens	136-141
34991674-9	2022	Additionally, we identified AHSA1-K137 as the specific binding site of Bufalin on AHSA1, mutation of which decreased the interaction of AHSA1 with HSP90A and suppressed the function of AHSA1 on mediating CDK6 and PSMD2.	bufalin	71-78	heat shock protein 90 alpha family class A member 1	Homo sapiens	147-153
34991674-9	2022	Additionally, we identified AHSA1-K137 as the specific binding site of Bufalin on AHSA1, mutation of which decreased the interaction of AHSA1 with HSP90A and suppressed the function of AHSA1 on mediating CDK6 and PSMD2.	bufalin	71-78	activator of HSP90 ATPase activity 1	Homo sapiens	185-190
34991674-9	2022	Additionally, we identified AHSA1-K137 as the specific binding site of Bufalin on AHSA1, mutation of which decreased the interaction of AHSA1 with HSP90A and suppressed the function of AHSA1 on mediating CDK6 and PSMD2.	bufalin	71-78	cyclin dependent kinase 6	Homo sapiens	204-208
34991674-9	2022	Additionally, we identified AHSA1-K137 as the specific binding site of Bufalin on AHSA1, mutation of which decreased the interaction of AHSA1 with HSP90A and suppressed the function of AHSA1 on mediating CDK6 and PSMD2.	bufalin	71-78	proteasome 26S subunit ubiquitin receptor, non-ATPase 2	Homo sapiens	213-218
34000344-0	2021	Bufalin targets the SRC-3/MIF pathway in chemoresistant cells to regulate M2 macrophage polarization in colorectal cancer.	bufalin	0-7	nuclear receptor coactivator 3	Homo sapiens	20-25
34000344-0	2021	Bufalin targets the SRC-3/MIF pathway in chemoresistant cells to regulate M2 macrophage polarization in colorectal cancer.	bufalin	0-7	macrophage migration inhibitory factor	Homo sapiens	26-29
34000344-5	2021	Furthermore, we demonstrated that Bufalin targeted the SRC-3 protein to reduce MIF release in chemoresistant cells in order to regulate the polarization of M2 macrophages.	bufalin	34-41	nuclear receptor coactivator 3	Homo sapiens	55-60
34000344-5	2021	Furthermore, we demonstrated that Bufalin targeted the SRC-3 protein to reduce MIF release in chemoresistant cells in order to regulate the polarization of M2 macrophages.	bufalin	34-41	macrophage migration inhibitory factor	Homo sapiens	79-82
33913201-9	2021	NOS3, which was an independent prognostic factor of GC patients, was predicted to be a potential target of bufalin.	bufalin	107-114	nitric oxide synthase 3	Homo sapiens	0-4
33913201-10	2021	Further experiments proved that bufalin reduced the phosphorylation of NOS3, thereby inhibiting the mitogen-activated protein kinase (MAPK) signaling pathway, and ultimately suppressed GCPD by inhibiting EMT process.	bufalin	32-39	nitric oxide synthase 3	Homo sapiens	71-75
33913201-12	2021	Bufalin could suppress GCPD through NOS3-MAPK signaling pathway, which provided more evidence support for intraperitoneal perfusion of bufalin to treat GCPD.	bufalin	0-7	nitric oxide synthase 3	Homo sapiens	36-40
33913201-12	2021	Bufalin could suppress GCPD through NOS3-MAPK signaling pathway, which provided more evidence support for intraperitoneal perfusion of bufalin to treat GCPD.	bufalin	135-142	nitric oxide synthase 3	Homo sapiens	36-40
33124657-0	2021	Bufalin inhibits human diffuse large B cell lymphoma tumorigenesis by inducing cell death through the Ca 2+/NFATC1/cMYC pathway.	bufalin	0-7	nuclear factor of activated T cells 1	Homo sapiens	108-114
33124657-0	2021	Bufalin inhibits human diffuse large B cell lymphoma tumorigenesis by inducing cell death through the Ca 2+/NFATC1/cMYC pathway.	bufalin	0-7	MYC proto-oncogene, bHLH transcription factor	Homo sapiens	115-119