PMID-sentid Pub_year Sent_text compound_name comp_offset prot_official_name organism prot_offset 12740379-11 2003 In cells preloaded with 32Pi, the phosphorylation of PASK, but not DNPASK, coincides with that of NKCC1 and increases 5.5 +/- 0.36-fold in low [Cl]e. These data conclusively link PASK with the phosphorylation and activation of NKCC1. 32pi 24-28 serine/threonine kinase 39 Homo sapiens 53-57 16113079-2 2005 Dual uptake (32Pi/22Na) assays confirmed that NaPi-IIc displayed Na+-coupled Pi cotransport with a 2:1 (Na+:Pi) stoichiometry compared with 3:1 established for NaPi-IIa. 32pi 13-17 solute carrier family 34 member 3 Homo sapiens 46-54 12887697-5 2003 Both basal and PKC-mediated labeling of Goalpha with 32Pi was significantly less in DRGs from diabetic rats, supporting increased endogenous PKC-dependent phosphorylation of Goalpha. 32pi 53-57 protein kinase C, alpha Rattus norvegicus 15-18 12887697-5 2003 Both basal and PKC-mediated labeling of Goalpha with 32Pi was significantly less in DRGs from diabetic rats, supporting increased endogenous PKC-dependent phosphorylation of Goalpha. 32pi 53-57 protein kinase C, alpha Rattus norvegicus 141-144 12740379-11 2003 In cells preloaded with 32Pi, the phosphorylation of PASK, but not DNPASK, coincides with that of NKCC1 and increases 5.5 +/- 0.36-fold in low [Cl]e. These data conclusively link PASK with the phosphorylation and activation of NKCC1. 32pi 24-28 solute carrier family 12 member 2 Homo sapiens 98-103 12740379-11 2003 In cells preloaded with 32Pi, the phosphorylation of PASK, but not DNPASK, coincides with that of NKCC1 and increases 5.5 +/- 0.36-fold in low [Cl]e. These data conclusively link PASK with the phosphorylation and activation of NKCC1. 32pi 24-28 solute carrier family 12 member 2 Homo sapiens 227-232 9321836-1 1997 We compared baseline and protein kinase A (PKA)-dependent troponin I (TnI) phosphorylation in 32Pi-labeled left ventricular myocytes from hearts of 26-wk spontaneously hypertensive rats (SHR) and Wistar-Kyoto controls (WKY). 32pi 94-98 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 25-41 10220564-3 1999 Immunoprecipitation of SPR from 32Pi-labeled Chinese hamster ovary cells stably expressing human SPR (CHO-hSPR) indicates that substance P (SP) causes a rapid (T1/2 < 1 min), dose-dependent (EC50 = 2 nM), and pronounced (5-fold over basal) phosphorylation of SPR. 32pi 32-36 tachykinin receptor 1 Homo sapiens 23-26 10220564-3 1999 Immunoprecipitation of SPR from 32Pi-labeled Chinese hamster ovary cells stably expressing human SPR (CHO-hSPR) indicates that substance P (SP) causes a rapid (T1/2 < 1 min), dose-dependent (EC50 = 2 nM), and pronounced (5-fold over basal) phosphorylation of SPR. 32pi 32-36 tachykinin receptor 1 Homo sapiens 97-100 10220564-3 1999 Immunoprecipitation of SPR from 32Pi-labeled Chinese hamster ovary cells stably expressing human SPR (CHO-hSPR) indicates that substance P (SP) causes a rapid (T1/2 < 1 min), dose-dependent (EC50 = 2 nM), and pronounced (5-fold over basal) phosphorylation of SPR. 32pi 32-36 tachykinin precursor 1 Homo sapiens 127-138 10220564-3 1999 Immunoprecipitation of SPR from 32Pi-labeled Chinese hamster ovary cells stably expressing human SPR (CHO-hSPR) indicates that substance P (SP) causes a rapid (T1/2 < 1 min), dose-dependent (EC50 = 2 nM), and pronounced (5-fold over basal) phosphorylation of SPR. 32pi 32-36 tachykinin precursor 1 Homo sapiens 23-25 10220564-3 1999 Immunoprecipitation of SPR from 32Pi-labeled Chinese hamster ovary cells stably expressing human SPR (CHO-hSPR) indicates that substance P (SP) causes a rapid (T1/2 < 1 min), dose-dependent (EC50 = 2 nM), and pronounced (5-fold over basal) phosphorylation of SPR. 32pi 32-36 tachykinin receptor 1 Homo sapiens 97-100 10191361-2 1999 Mutations of any of these two Y nearly abolished the NaPi2 mediated 32Pi-uptake after cRNA-injection into oocytes. 32pi 68-72 solute carrier family 34 member 1 Rattus norvegicus 53-58 9873045-4 1999 Activation of PDX1 appears to involve phosphorylation, as shown by incorporation of 32Pi into the 46-kDa form of the protein. 32pi 84-88 pancreatic and duodenal homeobox 1 Homo sapiens 14-18 9716607-7 1998 Quantitatively, all bcl-2 from ATRA-treated cells was in the phosphorylated isoform, while after taxol, both phosphorylated and native bcl-2 was present; incorporation of 32Pi into bcl-2 was stimulated to greater extent in ATRA-treated compared with taxol-treated cells. 32pi 171-175 BCL2 apoptosis regulator Homo sapiens 20-25 9657743-4 1998 Tyrosine phosphorylation of STAT5 is accompanied by the translocation of activated STAT5 to the nucleus as shown by electrophoretic mobility shift assay (EMSA) using 32Pi-labeled STAT5 binding site in the beta-casein promoter. 32pi 166-170 signal transducer and activator of transcription 5A Homo sapiens 28-33 9657743-4 1998 Tyrosine phosphorylation of STAT5 is accompanied by the translocation of activated STAT5 to the nucleus as shown by electrophoretic mobility shift assay (EMSA) using 32Pi-labeled STAT5 binding site in the beta-casein promoter. 32pi 166-170 signal transducer and activator of transcription 5A Homo sapiens 83-88 9657743-4 1998 Tyrosine phosphorylation of STAT5 is accompanied by the translocation of activated STAT5 to the nucleus as shown by electrophoretic mobility shift assay (EMSA) using 32Pi-labeled STAT5 binding site in the beta-casein promoter. 32pi 166-170 signal transducer and activator of transcription 5A Homo sapiens 83-88 9637070-7 1998 Although pho2 mutants could recycle 32Pi from shoots to roots through phloem the proportion of 32Pi translocated to roots was less than half of that found in wild-type plants. 32pi 36-40 phosphate 2 Arabidopsis thaliana 9-13 9409484-10 1997 Endothelin caused an increase in the level of incorporation of 32Pi into myosin light chain 2 without a concurrent change in the level of incorporation of 32Pi into troponin I. 32pi 63-67 myosin light chain 2 Rattus norvegicus 73-93 9321836-1 1997 We compared baseline and protein kinase A (PKA)-dependent troponin I (TnI) phosphorylation in 32Pi-labeled left ventricular myocytes from hearts of 26-wk spontaneously hypertensive rats (SHR) and Wistar-Kyoto controls (WKY). 32pi 94-98 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 43-46 9321836-1 1997 We compared baseline and protein kinase A (PKA)-dependent troponin I (TnI) phosphorylation in 32Pi-labeled left ventricular myocytes from hearts of 26-wk spontaneously hypertensive rats (SHR) and Wistar-Kyoto controls (WKY). 32pi 94-98 troponin I3, cardiac type Rattus norvegicus 58-68 9321836-1 1997 We compared baseline and protein kinase A (PKA)-dependent troponin I (TnI) phosphorylation in 32Pi-labeled left ventricular myocytes from hearts of 26-wk spontaneously hypertensive rats (SHR) and Wistar-Kyoto controls (WKY). 32pi 94-98 troponin I3, cardiac type Rattus norvegicus 70-73 8798774-6 1996 ODC was metabolically radiolabeled with 32Pi in RAW264 cells and also subjected to proteolysis, two-dimensional peptide mapping, and phosphoamino acid analysis. 32pi 40-44 ornithine decarboxylase, structural 1 Mus musculus 0-3 9101429-7 1997 Stimulation of cells with interleukin-1 beta enhanced the synthesis of both fluorescent PI (approximately 88%) and PC (approximately 250%) compared to non-stimulated cells, but with less incorporation of 32Pi into fluorescent PI. 32pi 204-208 interleukin 1 beta Homo sapiens 26-44 8955135-7 1996 In the present study, we demonstrate that BCR Tyr-246 and at least one of the closely spaced tyrosine residues, Tyr-279, Tyr-283, and Tyr-289 (3Y cluster), are phosphorylated by FES both in vitro and in 32Pi-labeled cells. 32pi 203-207 BCR activator of RhoGEF and GTPase Homo sapiens 42-45 8944652-3 1996 Autoradiograms of sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of myosin immunoprecipitated from 32Pi-labeled transfected cells demonstrated that MLC20 phosphorylation was increased 3.1 +/- 0.9-fold in cells expressing tMK compared with cells expressing beta-gal. 32pi 119-123 deoxythymidylate kinase Mus musculus 241-244 8879142-8 1996 Immunoprecipitation followed by SDS-PAGE demonstrated a single 32Pi-labeled UL3 related band with an apparent molecular weight of 33 kDa, indicating that the UL3 protein was a phosphoprotein. 32pi 63-67 nuclear protein UL3 Human alphaherpesvirus 1 76-79 8879142-8 1996 Immunoprecipitation followed by SDS-PAGE demonstrated a single 32Pi-labeled UL3 related band with an apparent molecular weight of 33 kDa, indicating that the UL3 protein was a phosphoprotein. 32pi 63-67 nuclear protein UL3 Human alphaherpesvirus 1 158-161 7961831-2 1994 Incorporation of 32Pi into phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine in wild type and ept1 strains was decreased in the presence of exogenous inositol. 32pi 17-21 bifunctional diacylglycerol cholinephosphotransferase/ethanolaminephosphotransferase Saccharomyces cerevisiae S288C 114-118 8702918-8 1996 268, 15523-15530), we obtained a cytosolic fraction from A431 cells that specifically dephosphorylated 32Pi-labeled c-Jun protein used as substrate in an immunocomplex phosphatase assay. 32pi 103-107 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 116-121 7758155-6 1995 Incubation with either PMA, AA, or ET resulted in similar increases in 32Pi incorporation into troponin I (TnI) and myosin light chain 2 (MLC2), which was inhibited by preincubation with the protein kinase C antagonist calphostin C. In addition, the ability of these agonists to stimulate phosphorylation of TnI or MLC2 did not require extracellular Ca2+ or intact intracellular Ca2+ stores. 32pi 71-75 troponin I3, cardiac type Rattus norvegicus 95-105 7758155-6 1995 Incubation with either PMA, AA, or ET resulted in similar increases in 32Pi incorporation into troponin I (TnI) and myosin light chain 2 (MLC2), which was inhibited by preincubation with the protein kinase C antagonist calphostin C. In addition, the ability of these agonists to stimulate phosphorylation of TnI or MLC2 did not require extracellular Ca2+ or intact intracellular Ca2+ stores. 32pi 71-75 troponin I3, cardiac type Rattus norvegicus 107-110 7758155-6 1995 Incubation with either PMA, AA, or ET resulted in similar increases in 32Pi incorporation into troponin I (TnI) and myosin light chain 2 (MLC2), which was inhibited by preincubation with the protein kinase C antagonist calphostin C. In addition, the ability of these agonists to stimulate phosphorylation of TnI or MLC2 did not require extracellular Ca2+ or intact intracellular Ca2+ stores. 32pi 71-75 troponin I3, cardiac type Rattus norvegicus 308-311 7758155-6 1995 Incubation with either PMA, AA, or ET resulted in similar increases in 32Pi incorporation into troponin I (TnI) and myosin light chain 2 (MLC2), which was inhibited by preincubation with the protein kinase C antagonist calphostin C. In addition, the ability of these agonists to stimulate phosphorylation of TnI or MLC2 did not require extracellular Ca2+ or intact intracellular Ca2+ stores. 32pi 71-75 myosin light chain 2 Rattus norvegicus 315-319 7668376-2 1995 One of the key reaction steps of this assay is the polynucleotide kinase (PNK)-catalyzed incorporation of 32Pi into nucleotides. 32pi 106-110 polynucleotide kinase 3'-phosphatase Homo sapiens 51-72 7668376-2 1995 One of the key reaction steps of this assay is the polynucleotide kinase (PNK)-catalyzed incorporation of 32Pi into nucleotides. 32pi 106-110 polynucleotide kinase 3'-phosphatase Homo sapiens 74-77 7698356-7 1995 Labelling of PEPCK by 32Pi in darkened cotyledons was reversed by illumination. 32pi 22-26 phosphoenolpyruvate carboxykinase (ATP) Cucumis sativus 13-18 8626435-5 1996 Using two-dimensional tryptic phosphopeptide mapping, we studied the phosphorylation of p47phox in 32Pi-loaded Epstein-Barr virus-transformed B lymphoblasts expressing wild type p47phox or any of several P47phox Ser -> Ala mutants. 32pi 99-103 neutrophil cytosolic factor 1 Homo sapiens 88-95 8603921-3 1996 Both isoforms incorporate 32Pi in vivo, suggesting that Spc110p is post-translationally modified by phosphorylation. 32pi 26-30 Spc110p Saccharomyces cerevisiae S288C 56-63 7613626-3 1995 In situ phosphorylation experiments performed with 32Pi-labelled synaptosomes from the affected areas demonstrated that the phosphorylation of the nervous tissue-specific presynaptic membrane-associated protein kinase C substrate B-50/GAP-43 was increased by 51.4 and 44.8% in cortex and hippocampus respectively. 32pi 51-55 growth associated protein 43 Rattus norvegicus 235-241 7536193-1 1995 We previously reported that when 32Pi-loaded rat parotid slices are incubated with the beta-adrenergic agonist isoproterenol, the level of a soluble 32P-labeled 17-kDa protein (pp17) decreases rapidly (Kanamori, T., and Hayakawa, T. (1982) Biochem. 32pi 33-37 stathmin 1 Rattus norvegicus 177-181 8035796-3 1994 32Pi labeling and isoelectric focusing analysis of a SUI2+ delta gcn2 strain identifies eIF-2 alpha as radiolabeled and a single isoelectric protein species. 32pi 0-4 translation initiation factor eIF2 subunit alpha Saccharomyces cerevisiae S288C 53-57 7929054-7 1994 Pulse labeling of cells metabolically arrested in G1,S, or G2/M with 32Pi showed that pol delta is a phosphoprotein and that it is most actively phosphorylated during the S phase. 32pi 69-73 DNA polymerase delta 1, catalytic subunit Homo sapiens 86-95 8035796-3 1994 32Pi labeling and isoelectric focusing analysis of a SUI2+ delta gcn2 strain identifies eIF-2 alpha as radiolabeled and a single isoelectric protein species. 32pi 0-4 serine/threonine-protein kinase GCN2 Saccharomyces cerevisiae S288C 65-69 1500712-5 1992 Quantitative analysis of Op18 phosphorylation was undertaken by metabolic labeling with 32Pi and PhosphorImager analysis of two-dimensional gels. 32pi 88-92 stathmin 1 Homo sapiens 25-29 7908287-5 1994 When similar incubations were performed with 32Pi-labeled cells and the receptors were immunoprecipitated with specific antibodies, alpha 2-C10 and alpha 2-C2 receptors were found to undergo an approximately 3-fold increase in receptor phosphorylation after epinephrine exposure. 32pi 45-49 adrenoceptor alpha 2A Homo sapiens 132-143 7908287-5 1994 When similar incubations were performed with 32Pi-labeled cells and the receptors were immunoprecipitated with specific antibodies, alpha 2-C10 and alpha 2-C2 receptors were found to undergo an approximately 3-fold increase in receptor phosphorylation after epinephrine exposure. 32pi 45-49 adrenergic receptor, alpha 2b Mus musculus 148-158 8114616-11 1993 To further investigate the effect of acute amphetamine, the ability of amphetamine to alter neuromodulin phosphorylation in 32Pi-preincubated Percoll-purified rat striatal synaptosomes was examined. 32pi 124-128 growth associated protein 43 Rattus norvegicus 92-104 8494789-3 1993 To explore whether this mobility shift was caused by phosphorylation, PTP1B was immunoprecipitated from 32Pi-labeled unsynchronized and mitotic HeLa cells. 32pi 104-108 protein tyrosine phosphatase non-receptor type 1 Homo sapiens 70-75 8380078-7 1993 Transfections in the presence of 32Pi demonstrated that the mature forms of both gpI and gpIV were phosphorylated, while similar experiments with [35S]sulfate showed that only the mature gpI was sulfated. 32pi 33-37 glucose-6-phosphate isomerase Homo sapiens 81-84 8380078-7 1993 Transfections in the presence of 32Pi demonstrated that the mature forms of both gpI and gpIV were phosphorylated, while similar experiments with [35S]sulfate showed that only the mature gpI was sulfated. 32pi 33-37 glycoprotein VI platelet Homo sapiens 89-93 8380078-7 1993 Transfections in the presence of 32Pi demonstrated that the mature forms of both gpI and gpIV were phosphorylated, while similar experiments with [35S]sulfate showed that only the mature gpI was sulfated. 32pi 33-37 glucose-6-phosphate isomerase Homo sapiens 89-92 7514867-3 1994 Metabolic labelling of resting platelets with 32Pi showed that P-selectin is primarily phosphorylated on serine residues, although some tyrosine phosphorylation was observed as well. 32pi 46-50 selectin P Homo sapiens 63-73 8453734-2 1993 Here we show in rat osteoblast-like cells (UMR 106-06) that PTH (10(-11) to 10(-7) M) increases both 32Pi uptake and cellular [Pi] by up to 50 per cent. 32pi 101-105 parathyroid hormone Rattus norvegicus 60-63 1316838-2 1992 32P-labeled phenylalanine hydroxylase was immunoisolated from cells loaded with 32Pi or from cell extracts "back-phosphorylated" with [gamma-32P]ATP by cAMP-dependent protein kinase. 32pi 80-84 phenylalanine hydroxylase Rattus norvegicus 12-37 1377674-7 1992 CFTR was immunoprecipitated from T84 cells labeled with 32Pi. 32pi 56-60 CF transmembrane conductance regulator Homo sapiens 0-4 1587853-3 1992 Expression of a rap1GAP cDNA in insect cells labeled with 32Pi resulted in high level incorporation of radioactivity into serine residues of the expressed protein. 32pi 58-62 RAP1 GTPase activating protein Homo sapiens 16-23 2174878-2 1990 There was a progressive release of 32Pi from [gamma-32P]ATP when GDP-bound G0 was incubated together with NDP kinase. 32pi 35-39 cytidine/uridine monophosphate kinase 2 Homo sapiens 106-116 1345770-1 1992 Tryptic digestion of tyrosine hydroxylase (TH) isolated from rat adrenal glands labeled with 32Pi produced five phosphopeptides. 32pi 93-97 tyrosine hydroxylase Rattus norvegicus 21-41 1345770-1 1992 Tryptic digestion of tyrosine hydroxylase (TH) isolated from rat adrenal glands labeled with 32Pi produced five phosphopeptides. 32pi 93-97 tyrosine hydroxylase Rattus norvegicus 43-45 1910317-6 1991 The in vivo phosphorylation of Grp78 was studied in MDBK cells labeled with 32Pi. 32pi 76-80 heat shock protein family A (Hsp70) member 5 Bos taurus 31-36 1861147-4 1991 However, when we used cells prelabelled with 32Pi for 2.5 h, we found that bradykinin produced a substantial stimulation of label found in PA, with an EC50 value of about 1 nM. 32pi 45-49 kininogen 1 Bos taurus 75-85 1861148-1 1991 Using primary cultures of bovine adrenal chromaffin cells labelled with 32Pi, we show that stimulation with bradykinin, nicotine, or a depolarising concentration of potassium stimulates the accumulation of [32P]phosphatidic acid. 32pi 72-76 kininogen 1 Bos taurus 108-118 1828070-0 1991 ATP in equilibrium with 32Pi exchange catalyzed by plasma membrane Ca(2+)-ATPase from kidney proximal tubules. 32pi 24-28 ATPase plasma membrane Ca2+ transporting 2 Homo sapiens 51-80 1729391-3 1992 We have previously shown that nicotine stimulates incorporation of 32Pi into the vesicle-associated phosphoprotein synapsin II. 32pi 67-71 synapsin II Bos taurus 115-126 1729391-4 1992 We report here that histamine, too, stimulates an increase in 32Pi incorporation into synapsin II, which is blocked by the H1-histamine receptor-specific antagonist pyrilamine. 32pi 62-66 synapsin II Bos taurus 86-97 1672315-6 1991 After 32Pi labeling of rat corpus striata in vivo or rat corpus striatal synaptosomes, 32P incorporation into tyrosine hydroxylase occurred predominantly at serines 19, 31, and 40. 32pi 6-10 tyrosine hydroxylase Rattus norvegicus 110-130 1824695-11 1991 Neurogranin was shown to be phosphorylated in hippocampal slices incubated with 32Pi and phorbol esters stimulated neurogranin phosphorylation, suggesting that neurogranin is likely to be an in vivo substrate for PKC. 32pi 80-84 neurogranin Bos taurus 0-11 1824695-11 1991 Neurogranin was shown to be phosphorylated in hippocampal slices incubated with 32Pi and phorbol esters stimulated neurogranin phosphorylation, suggesting that neurogranin is likely to be an in vivo substrate for PKC. 32pi 80-84 neurogranin Bos taurus 160-171 2501296-8 1989 TRH (10(-7) M), which activates protein kinase C via a receptor-mediated mechanism, similarly stimulated 32Pi incorporation into sphingomyelin at a rate 1.5-fold of control; EC50 congruent to 5 x 10(-10) M TRH. 32pi 105-109 thyrotropin releasing hormone Rattus norvegicus 0-3 2295696-5 1990 32Pi incorporation studies indicate that L4 is an unphosphorylated form and L2 is a phosphorylated form of hsp27. 32pi 0-4 heat shock protein family B (small) member 1 Homo sapiens 107-112 2246237-3 1990 This discrepancy between the increase in the fraction of phosphorylated eIF-4E and the increase in 32Pi labeling suggested that the phosphoryl group of eIF-4E turns over slowly in resting B cells compared with activated cells. 32pi 99-103 eukaryotic translation initiation factor 4E Mus musculus 152-158 2394752-4 1990 32P-Labeled rat retina phosducin was isolated by immunoprecipitation either after phosphorylation by protein kinase A in the presence of [gamma-32P]ATP or after incubation of retinas in darkness with 32Pi. 32pi 200-204 phosducin Bos taurus 23-32 2154685-4 1990 In addition, p21 and TPA, but not insulin or progesterone, stimulated the incorporation of 32Pi into oocyte lipids. 32pi 91-95 cyclin-dependent kinase inhibitor 1A L homeolog Xenopus laevis 13-16 3334749-1 1988 We detected phosphorylation of the major Moloney murine leukemia virus (M-MuLV) capsid polypeptide, p30, by using 32Pi-labeled virions. 32pi 114-118 centromere protein V Homo sapiens 100-103 2525929-7 1989 The assembled complex, however, exhibited reduced [32Pi]ATP exchange activity and low sensitivity to oligomycin, indicating that the product of the aap1 gene is a functional subunit of the mitochondrial H+-ATPase. 32pi 51-55 arginine/alanine aminopeptidase Saccharomyces cerevisiae S288C 148-152 2492151-8 1989 Incorporation of 32Pi into eIF-2 alpha was two- to threefold higher in lysine-deprived cells than in hepatocytes incubated in fully supplemented medium. 32pi 17-21 eukaryotic translation initiation factor 2A Rattus norvegicus 27-38 2561651-4 1989 Carbachol-induced a stimulation of the PPI turnover cycle: namely, a decrease in 32Pi incorporation into phosphatidylinositol-4,5-bisphosphate (TPI) and phosphatidylinositol-4-phosphate (DPI), and an increase in this incorporation into phosphatidylinositol (PI) and phosphatidic acid (PA) in rat brain synaptosomes from the cerebrum. 32pi 81-85 triosephosphate isomerase 1 Rattus norvegicus 144-147 2521182-2 1989 B-50 was phosphorylated in intact hippocampal slices with 32Pi or in synaptosomal plasma membranes with [gamma-32P]ATP. 32pi 58-62 growth associated protein 43 Homo sapiens 0-4 2843104-12 1988 When human neutrophil phosphoinositides were prelabeled with 32Pi, subsequent incubation with ACP2 resulted in an 85% loss of the labeled PIP2 over 2 h. Following fMLP stimulation of [3H]inositol-labeled neutrophils, the quantity of IP3 produced by ACP2-treated cells was reduced by 44%. 32pi 61-65 acid phosphatase 2, lysosomal Homo sapiens 94-98 2843104-12 1988 When human neutrophil phosphoinositides were prelabeled with 32Pi, subsequent incubation with ACP2 resulted in an 85% loss of the labeled PIP2 over 2 h. Following fMLP stimulation of [3H]inositol-labeled neutrophils, the quantity of IP3 produced by ACP2-treated cells was reduced by 44%. 32pi 61-65 formyl peptide receptor 1 Homo sapiens 163-167 3275664-0 1988 Insulin stimulates incorporation of 32Pi into nuclear lamins A and C in quiescent BHK-21 cells. 32pi 36-40 insulin Mesocricetus auratus 0-7 3275664-1 1988 The in vivo effect of insulin upon the incorporation of 32Pi into nuclear proteins was investigated in quiescent baby hamster kidney fibroblasts (BHK-21). 32pi 56-60 insulin Mesocricetus auratus 22-29 2848242-1 1988 [32P]pp15, the [32P]phosphorylated form of a specific cytosolic substrate of the insulin receptor tyrosine kinase, was purified to homogeneity from mouse 3T3-L1 adipocytes incubated with 32Pi. 32pi 187-191 nuclear transport factor 2 Mus musculus 5-9 2853304-6 1988 When cells were pulsed with 32Pi for 60 min and stimulated by veratridine plus scorpion venom for an additional 30 min, uptake of 32Pi into phosphatidylinositol was reduced; stimulating cells with bradykinin, a receptor agonist which activates the inositol cycle, promoted a 3.8 fold increase. 32pi 28-32 kininogen 1 Homo sapiens 197-207 2853304-6 1988 When cells were pulsed with 32Pi for 60 min and stimulated by veratridine plus scorpion venom for an additional 30 min, uptake of 32Pi into phosphatidylinositol was reduced; stimulating cells with bradykinin, a receptor agonist which activates the inositol cycle, promoted a 3.8 fold increase. 32pi 130-134 kininogen 1 Homo sapiens 197-207 3334749-4 1988 When immunoprecipitates from M-MuLV lysates labeled with 32Pi were compared with those labeled with [35S]methionine, it was calculated that the degree of phosphorylation at the p30 domain of Pr65gag was only 0.22 to 0.54% relative to phosphorylation at the p12 domain. 32pi 57-61 centromere protein V Homo sapiens 177-180 3334749-9 1988 Based on a comparison of specific labeling of p30 tryptic peptides with [14C]serine, [35S]methionine, and 32Pi, we tentatively assigned the phosphorylation site to a 2.4-kilodalton NH2-terminal peptide containing triple tandem serines spanning the region from amino acids 4 to 24. 32pi 106-110 centromere protein V Homo sapiens 46-49 2957366-5 1987 The 32Pi incorporated into calmodulin was stable to base, indicating that phosphotyrosine was involved and thus implicating the insulin-receptor tyrosine kinase as being responsible for its phosphorylation. 32pi 4-8 calmodulin 1 Homo sapiens 27-37 2957366-5 1987 The 32Pi incorporated into calmodulin was stable to base, indicating that phosphotyrosine was involved and thus implicating the insulin-receptor tyrosine kinase as being responsible for its phosphorylation. 32pi 4-8 insulin Homo sapiens 128-135 2444274-8 1987 The increase in Ca2+in up to 100 microM is concomitant with the activation of 32Pi incorporation into band 4.1 protein. 32pi 78-82 erythrocyte membrane protein band 4.1 Homo sapiens 102-110 3040878-8 1987 PAF stimulation of cultured hepatocytes prelabeled with 32Pi induced a transient decrease of [32P]polyphosphoinositides at 20 sec to 1 min. 32pi 56-60 PCNA clamp associated factor Rattus norvegicus 0-3 2887563-2 1987 The regulation of pineal phospholipase A2 activity was studied indirectly by measuring the release of [3H]arachidonic acid from [3H]arachidonic acid-labeled tissue in organ culture and the formation of radiolabeled lysophosphatidylcholine by glands labeled with 32Pi or [14C]choline. 32pi 262-266 phospholipase A2 group IB Homo sapiens 25-41 2887563-10 1987 The effects of norepinephrine, A23187, and protein kinase C activators appear to be mediated by phospholipase A2 because the effects of these compounds on [3H]arachidonic acid release are blocked by an established inhibitor of this enzyme, mepacrine, and because these compounds stimulate the formation of 32P- and 14C-labeled lysophosphatidylcholine by glands incubated with 32Pi or [14C]choline. 32pi 376-380 phospholipase A2 group IB Homo sapiens 96-112 3536709-2 1986 L6 cells readily incorporated 32Pi into a number of peptides approaching steady-state incorporation by 2 h. Insulin stimulated the phosphorylation of one peptide of molecular mass 29,000 daltons by 37% with an ED50 of 3 mU/ml. 32pi 30-34 insulin Homo sapiens 108-115 3107554-5 1987 Treatment of steady state 32Pi-labelled cells with EGF stimulated total and alkali-stable phosphorylation in the 97 kDa receptor band by about 35%. 32pi 26-30 epidermal growth factor Homo sapiens 51-54 3558493-5 1987 Pretreatment of cells with phorbol-12, 13-dibutyrate (PDBu) for 24 h depleted cellular protein kinase C activity and inhibited the ability of TPA to induce these effects suggesting a direct involvement of protein kinase C. Similarly the bombesin stimulation of 32Pi into PC and of [3H]choline and [3H]phosphocholine release was inhibited by PDBu pretreatment. 32pi 261-265 gastrin releasing peptide Homo sapiens 237-245 3025207-5 1987 Changes in thrombin binding correlated closely with changes in thrombin-stimulated incorporation of 32Pi into phosphatidic acid and a 40-kDa protein. 32pi 100-104 coagulation factor II, thrombin Homo sapiens 11-19 3025207-5 1987 Changes in thrombin binding correlated closely with changes in thrombin-stimulated incorporation of 32Pi into phosphatidic acid and a 40-kDa protein. 32pi 100-104 coagulation factor II, thrombin Homo sapiens 63-71 3821186-1 1986 When quiescent young or senescent human diploid cells, TIG-1, were metabolically labeled with 32Pi and stimulated with 10% fetal bovine serum, the phosphorylation of ribosomal S6 protein was enhanced in young cells but not in senescent cells while that of some other proteins were increased in both cells. 32pi 94-98 retinoic acid receptor responder 1 Homo sapiens 55-60 3022295-8 1986 In cells exposed to TRH for 4 min and then to 32Pi, 32P-labeling of PtdOH and PtdIns increased, but that of PtdIns(4,5)P2 was not affected. 32pi 46-50 thyrotropin releasing hormone Rattus norvegicus 20-23 3023921-5 1986 Pulse-labeling of the antibiotic-treated cultures with 32Pi showed a marked reduction of 32P radioactivity incorporated into p60src. 32pi 55-59 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 125-131 3565575-8 1987 However, in spite of a decreased capacity for Na+ gradient-dependent 32Pi uptake in BBMV-JM and much lesser in BBMV-SC in response to administration of calcitonin and PTH in Pi-deprived rats, these phosphaturic peptides did not increase FEPi. 32pi 69-73 calcitonin-related polypeptide alpha Rattus norvegicus 152-162 3565575-8 1987 However, in spite of a decreased capacity for Na+ gradient-dependent 32Pi uptake in BBMV-JM and much lesser in BBMV-SC in response to administration of calcitonin and PTH in Pi-deprived rats, these phosphaturic peptides did not increase FEPi. 32pi 69-73 parathyroid hormone Rattus norvegicus 167-170 2434494-2 1987 The activity of the thrice extracted particle (AE-P3) in ATP-driven NAD+ reduction by succinate and the 32Pi-ATP exchange activity were substantially stimulated, 8-fold and 5-fold, respectively, by purified FB. 32pi 104-108 distal membrane arm assembly component 2 like Bos taurus 207-209 3558493-0 1987 Bombesin and phorbol ester stimulate phosphatidylcholine hydrolysis by phospholipase C: evidence for a role of protein kinase C. Bombesin caused a marked stimulation of 32Pi into phosphatidylinositol (PI), with no apparent lag, and into phosphatidylcholine (PC), after a lag of about 20 min. 32pi 169-173 gastrin releasing peptide Homo sapiens 0-8 3558493-0 1987 Bombesin and phorbol ester stimulate phosphatidylcholine hydrolysis by phospholipase C: evidence for a role of protein kinase C. Bombesin caused a marked stimulation of 32Pi into phosphatidylinositol (PI), with no apparent lag, and into phosphatidylcholine (PC), after a lag of about 20 min. 32pi 169-173 gastrin releasing peptide Homo sapiens 129-137 3027087-7 1987 In vivo labeling with 32Pi showed that plectin was the target for cAMP-independent protein kinases which phosphorylated 18-kDa domains at the end(s) of the molecule. 32pi 22-26 plectin Homo sapiens 39-46 3104182-6 1987 When hepatocytes labelled with 32Pi were treated with vasopressin, no change in the amounts of [32P]lysophosphatidylethanolamine or [32P]lysophosphatidylcholine was observed. 32pi 31-35 arginine vasopressin Rattus norvegicus 54-65 3024158-5 1986 The enzyme catalyzed the transfer of 32Pi from [gamma-32P]ATP to gpI but not to gpII and gpIII. 32pi 37-41 glucose-6-phosphate isomerase Homo sapiens 65-68 3022295-3 1986 In cells incubated with 32Pi for 1 min, TRH rapidly and persistently (for at least 30 min) enhanced the rate of 32P-labeling of PtdOH. 32pi 24-28 thyrotropin releasing hormone Rattus norvegicus 40-43 3019777-1 1986 Threonyl-tRNA synthetase is phosphorylated in Chinese hamster ovary cells labeled with 32Pi [(1984) J. Biol. 32pi 87-91 threonine--tRNA ligase 1, cytoplasmic Cricetulus griseus 0-24 2424009-1 1986 Incubating 32P-labeled fat cells with insulin increased by as much as 80-fold the amount of 32Pi in a soluble species of apparent Mr 62,000. 32pi 92-96 insulin Bos taurus 38-45 2996433-4 1985 In isolated maize leaf mesophyll protoplasts preilluminated with 32Pi, we observed a three- to fivefold higher PPDK activity in situ in the light, and a corresponding three- to fivefold higher level of phosphorylation of the 94-kDa PPDK protomer in the dark. 32pi 65-69 pyruvate, phosphate dikinase 1, chloroplastic Zea mays 111-115 3002355-5 1985 ADH initially inhibited and subsequently stimulated 32Pi incorporation into phosphatidic acid (PA) and phosphatidylinositol (PI). 32pi 52-56 arginine vasopressin Homo sapiens 0-3 2996433-4 1985 In isolated maize leaf mesophyll protoplasts preilluminated with 32Pi, we observed a three- to fivefold higher PPDK activity in situ in the light, and a corresponding three- to fivefold higher level of phosphorylation of the 94-kDa PPDK protomer in the dark. 32pi 65-69 pyruvate, phosphate dikinase 1, chloroplastic Zea mays 232-236 4020958-5 1985 From pulse-labeling experiments with either 32Pi or [35S]methionine, NS-1 was found to first appear 5 to 7 h postinfection, whereas the viral structural polypeptides were first synthesized 9 to 11 h postinfection. 32pi 44-48 influenza virus NS1A binding protein Homo sapiens 69-73 3929257-6 1985 The stimulatory effect of TRH to increase 32Pi incorporation into phosphatidylinositol was decreased by phosphatidylserine. 32pi 42-46 thyrotropin releasing hormone Rattus norvegicus 26-29 6885823-12 1983 Two-dimensional gel electrophoresis of protein from human platelets that had been preincubated with 32Pi demonstrated the presence under control conditions of 2-3 major forms of P47 that contained very little 32P (pI values, 6.6-6.8) and, after induction of secretion with thrombin, their replacement by 7-9 highly labeled phosphorylated forms of P47 (pI values, 6.1-6.5). 32pi 100-104 pleckstrin Homo sapiens 178-181 3871471-6 1985 32Pi is incorporated into endogenous ATP and GTP pools twice as efficiently as in isolated OS, and is used in the phosphorylation of rhodopsin. 32pi 0-4 rhodopsin Homo sapiens 133-142 6388354-4 1984 Increased initial rates (15 s) of Na+-dependent 32Pi transport were measured in brush border vesicles prepared from segments that had been incubated with insulin. 32pi 48-52 insulin Canis lupus familiaris 154-161 6439189-2 1984 As early as 10s after addition of AVP (10(-6)M) a significant loss of radioactivity from phosphatidylinositol 4,5-bisphosphate but not from phosphatidylinositol 4-phosphate and phosphatidylinositol was observed in cells prelabelled with 32Pi. 32pi 237-241 arginine vasopressin Rattus norvegicus 34-37 6090133-0 1984 Enzymatic activities in thylakoid membranes, which form medium [32P]NDP and [32P]ATP from 32Pi. 32pi 90-94 norrin cystine knot growth factor NDP Homo sapiens 68-71 6432793-2 1984 Threonyl-tRNA synthetase was isolated by immunoprecipitation from cells labeled with 32Pi for 18 h. Phosphoamino acid analysis of radiolabeled threonyl-tRNA synthetase showed that phosphorylation occurs on serine. 32pi 85-89 threonine--tRNA ligase 1, cytoplasmic Cricetulus griseus 0-24 6432603-2 1984 In a 4-fold enriched population of gonadotrophs, 30 nM LHRH increased 32Pi incorporation into phosphatidic acid (PA) as early as 1 min after its addition. 32pi 70-74 gonadotropin releasing hormone 1 Homo sapiens 55-59 6326675-2 1984 The incorporation of 32Pi into the total phospholipid fraction of pituitary gonadotrophs was significantly elevated by 10(-8) M GnRH, with specific increases in the labeling of phosphatidylinositol and phosphatidic acid (PA). 32pi 21-25 gonadotropin releasing hormone 1 Rattus norvegicus 128-132 6583202-8 1984 Thus, induction of rapid 32Pi turnover in pp17 and pp27 may be an essential early signal in initiating and maintaining cellular effects of PMA. 32pi 25-29 perilipin 3 Homo sapiens 42-46 6228552-5 1983 Transformation with ATP2 restored the growth rate of S. pombe mutant on glycerol as well as the mitochondrial ATPase and 32Pi-ATP exchange activities to approximately 20% of the parental strain. 32pi 121-125 F1F0 ATP synthase subunit beta Saccharomyces cerevisiae S288C 20-24 6314912-2 1983 Na+-dependent 32Pi transport was decreased in brush border vesicles from isolated kidneys perfused with parathyroid hormone (PTH) for 2 h compared to uptake measured in vesicles from kidneys perfused without PTH. 32pi 14-18 parathyroid hormone Canis lupus familiaris 104-123 6314912-2 1983 Na+-dependent 32Pi transport was decreased in brush border vesicles from isolated kidneys perfused with parathyroid hormone (PTH) for 2 h compared to uptake measured in vesicles from kidneys perfused without PTH. 32pi 14-18 parathyroid hormone Canis lupus familiaris 125-128 6314912-7 1983 It is strongly suggested that PTH effects changes in Na+-dependent 32Pi transport in isolated brush border vesicles and changes in 32P phosphorylation of vesicles via a direct action on the renal cortical cell rather than as a consequence of extrarenal actions of the hormone. 32pi 67-71 parathyroid hormone Canis lupus familiaris 30-33 6432054-2 1984 In cells prelabelled with 32Pi it would be expected that the newly synthesised phosphatidate would have the same specific activity as cellular ATP, provided that the loss of phosphatidylinositol is by phospholipase C attack and the resultant diacyglycerol is phosphorylated by ATP. 32pi 26-30 LOC100009319 Oryctolagus cuniculus 201-216 6715348-2 1984 Vimentin, the subunit protein of one type of intermediate filament, has been isolated from 32Pi-labeled nonmitotic and mitotic mouse L-929 cells. 32pi 91-95 vimentin Mus musculus 0-8 6731846-5 1984 It is possible with this method to remove 32Pi (173 mumol) from ATP (50 mumol, 30 mg) in one run using a C-18 analytical column demonstrating that this approach can be useful for selected semipreparative purifications. 32pi 42-46 Bardet-Biedl syndrome 9 Homo sapiens 105-109 6417138-1 1983 The biosynthesis of arylsulfatase B in normal and mutant human skin fibroblasts was studied by metabolic labeling with radioactive amino acids, monosaccharides, or 32Pi and by specific immunoprecipitation followed by polyacrylamide gel electrophoresis and fluorography. 32pi 164-168 arylsulfatase B Homo sapiens 20-35 6873252-4 1983 The application of precursors of ATP biosynthesis, labeled with suitable radionuclides (32Pi and [14C]ADP), makes it possible to prove that ATP is formed in the absence of inhibitors. 32pi 88-92 ATPase phospholipid transporting 8A2 Homo sapiens 33-36 6873252-4 1983 The application of precursors of ATP biosynthesis, labeled with suitable radionuclides (32Pi and [14C]ADP), makes it possible to prove that ATP is formed in the absence of inhibitors. 32pi 88-92 ATPase phospholipid transporting 8A2 Homo sapiens 140-143 6131898-5 1983 F1-ATPase and Type II ATPase require F6 in addition to oligomycin sensitivity conferral protein and FB to reconstitute 32Pi-ATP exchange activity in silicotungstic acid particles. 32pi 119-123 ATP synthase peripheral stalk subunit F6 Homo sapiens 37-39 6131898-6 1983 This F6 requirement for the 32Pi-ATP exchange is not related to the F6 effect on the ATPase binding. 32pi 28-32 ATP synthase peripheral stalk subunit F6 Homo sapiens 5-7 6346945-2 1983 The first step in the synthesis involves the quantitative transfer of 32Pi from [gamma-32P]dATP to 5"-GMP catalyzed by GMP kinase. 32pi 70-74 5'-nucleotidase, cytosolic II Homo sapiens 102-105 6131898-7 1983 The Type I ATPase and therefore the 26,500-dalton subunit associated with it requires F6 and FB to reconstitute 32Pi-ATP exchange activity in silicotungstic acid particles. 32pi 112-116 ATP synthase peripheral stalk subunit F6 Homo sapiens 86-88 6346945-2 1983 The first step in the synthesis involves the quantitative transfer of 32Pi from [gamma-32P]dATP to 5"-GMP catalyzed by GMP kinase. 32pi 70-74 5'-nucleotidase, cytosolic II Homo sapiens 119-122 6296123-1 1983 The addition of thrombin to human platelets prelabeled with 32Pi led to significant loss of radioactivity in phosphatidylinositol 4,5-bisphosphate within 5 s, followed by recovery or even increase by 2 min. 32pi 60-64 coagulation factor II, thrombin Homo sapiens 16-24 6830253-5 1983 Intraperitoneally injected 32Pi was incorporated into the structural phosphate of ATP-citrate lyase in rat liver but not into the rat brain enzyme. 32pi 27-31 ATP citrate lyase Rattus norvegicus 82-99 6759164-1 1982 Glucagon and insulin both stimulated the 32P-labelling of ribosomal protein S6 in rat hepatocytes that had been incubated with 32Pi. 32pi 127-131 ribosomal protein S6 Rattus norvegicus 58-78 6367855-4 1983 [32Pi] incorporation into phospholipids is also stimulated by insulin. 32pi 1-5 insulin Homo sapiens 62-69 6286683-3 1982 32Pi introduced into MEP by metabolic labeling of intact cells is exclusively associated with asparagine-linked oligosaccharides as indicated by sensitivity to endohexosaminidase H. Labeling studies utilizing [2-3H]mannose indicate that approximately one-fifth of the mannose residues of MEP are phosphorylated. 32pi 0-4 cathepsin L Mus musculus 21-24 6959824-2 1982 When FELCs were incubated for short periods with 32Pi before cell fractionation, the lipid-bound radioactivity was almost exclusively present in phosphatidylinositol-4-phosphate (DPI) and phosphatidylinositol-4,5-bisphosphate (TPI), and its distribution closely matched that of the plasma membrane markers. 32pi 49-53 triosephosphate isomerase 1 Mus musculus 227-230 6292515-11 1982 The p21 coded for by a mouse-derived oncogenic virus, BALB murine sarcoma virus, resembled the p21 coded for by c-rasH-1 in that it bound guanine nucleotides but did not label appreciably with 32Pi. 32pi 193-197 transcription elongation factor A (SII)-like 1 Mus musculus 4-7 6292515-11 1982 The p21 coded for by a mouse-derived oncogenic virus, BALB murine sarcoma virus, resembled the p21 coded for by c-rasH-1 in that it bound guanine nucleotides but did not label appreciably with 32Pi. 32pi 193-197 transcription elongation factor A (SII)-like 1 Mus musculus 95-98 6286683-3 1982 32Pi introduced into MEP by metabolic labeling of intact cells is exclusively associated with asparagine-linked oligosaccharides as indicated by sensitivity to endohexosaminidase H. Labeling studies utilizing [2-3H]mannose indicate that approximately one-fifth of the mannose residues of MEP are phosphorylated. 32pi 0-4 cathepsin L Mus musculus 288-291 6178581-0 1982 Insulin stimulates the incorporation of 32Pi into ribonucleic acid in cultured sertoli cells. 32pi 40-44 insulin Homo sapiens 0-7 6178581-7 1982 Insulin-treated cells had approximately a 1.5-fold increased specific activity of [3H]uridine or 32Pi into the total nucleotide pool and in ATP, GTP, and UTP pools. 32pi 97-101 insulin Homo sapiens 0-7 6454964-1 1981 During the process of reconstitution of pig heart mitochondrial H+-ATPase on liposomes by cholate dialysis method, 1 mM Mg2+ in the dialysis medium can obviously increase activities of the reconstituted H+-ATPase (32Pi-ATP exchange, enzyme activity and its sensitivity to oligomycin or DCCD and ATP-dependent ANS 1) fluorescence). 32pi 214-218 ATPase H+ transporting V1 subunit A Sus scrofa 64-73 6272837-1 1981 Epidermal growth factor (EGF) stimulates the incorporation of 32Pi and [3H]inositol into phosphatidylinositol (5-10-fold) in A-431 cells. 32pi 62-66 epidermal growth factor Homo sapiens 0-23 6272837-1 1981 Epidermal growth factor (EGF) stimulates the incorporation of 32Pi and [3H]inositol into phosphatidylinositol (5-10-fold) in A-431 cells. 32pi 62-66 epidermal growth factor Homo sapiens 25-28 6272837-2 1981 EGF also stimulates the incorporation of 32Pi into phosphatidic acid (up to 10-fold). 32pi 41-45 epidermal growth factor Homo sapiens 0-3 6454964-1 1981 During the process of reconstitution of pig heart mitochondrial H+-ATPase on liposomes by cholate dialysis method, 1 mM Mg2+ in the dialysis medium can obviously increase activities of the reconstituted H+-ATPase (32Pi-ATP exchange, enzyme activity and its sensitivity to oligomycin or DCCD and ATP-dependent ANS 1) fluorescence). 32pi 214-218 ATPase H+ transporting V1 subunit A Sus scrofa 203-212 6454964-3 1981 The effectiveness of the divalent cation activation of 32Pi-ATP exchange of the reconstituted H+-ATPase, decreased in the order: Mg2+ greater than Ca2+ greater than Mn2+ greater than Sr2+, while that of increasing the sensitivity to the inhibitors: Ca2+ greater than Mg2+ greater than Mn2+ greater than Sr2+. 32pi 55-59 ATPase H+ transporting V1 subunit A Sus scrofa 94-103 144124-4 1977 The state 3 respiration, oxidative phosphorylation and 32Pi-ATP exchange reaction were inhibited by 10 to 50 nmol of CH3HgCl per mg of mitochondrial protein, while NADH-and succinate-dehydrogenase and ATPase were more resistant to it The difference spectrum of the treated mitochondria indicated that the point of inhibition was located after flavin and before cytochrome b. 32pi 56-60 dynein axonemal heavy chain 8 Homo sapiens 202-208 313567-1 1979 A protein of molecular weight 31,000 became labeled with 32P within 5 min after addition of insulin to differentiated 3T3-L1 preadipocytes previously incubated for 55 min with 32Pi. 32pi 176-180 insulin Homo sapiens 92-99 223211-2 1979 Morphine and beta-endorphin significantly increased microsomal 32PI incorporation into TPI and PI but not DPI. 32pi 63-67 triosephosphate isomerase 1 Rattus norvegicus 87-90 223211-6 1979 It was also found that chronic morphine treatment significantly increased the incorporation of 32Pi into synaptic TPI and DPI. 32pi 95-99 triosephosphate isomerase 1 Rattus norvegicus 114-117 27515-2 1978 When chick liver cells in monolayer culture were incubated with 32Pi in the presence of insulin, acetyl-CoA carboxylase became extensively labeled with 32Pi reaching a stoichiometry of 9 to 10 mol of phosphoryl group per mol of 240,000-dalton enzyme subunit. 32pi 64-68 insulin Gallus gallus 88-95 27515-2 1978 When chick liver cells in monolayer culture were incubated with 32Pi in the presence of insulin, acetyl-CoA carboxylase became extensively labeled with 32Pi reaching a stoichiometry of 9 to 10 mol of phosphoryl group per mol of 240,000-dalton enzyme subunit. 32pi 152-156 insulin Gallus gallus 88-95 6265172-2 1981 32Pi-labelled cells respond to epinephrine by increase in labeling of a 67,000-dalton band, presumably the activated lipase. 32pi 0-4 lipase G, endothelial type Rattus norvegicus 117-123 144124-4 1977 The state 3 respiration, oxidative phosphorylation and 32Pi-ATP exchange reaction were inhibited by 10 to 50 nmol of CH3HgCl per mg of mitochondrial protein, while NADH-and succinate-dehydrogenase and ATPase were more resistant to it The difference spectrum of the treated mitochondria indicated that the point of inhibition was located after flavin and before cytochrome b. 32pi 56-60 mitochondrially encoded cytochrome b Homo sapiens 362-374 1212220-13 1975 In a Ca2+-depleted buffer, the uptake of 32Pi is diminished, and hyperosmolarity, which stimulates 45Ca uptake, also accelerates the accumulation of 32Pi. 32pi 41-45 carbonic anhydrase 2 Rattus norvegicus 5-8 1260498-1 1976 Rat liver mitochondria which have been exposed to 0.15 M NaC1 at 35 degrees C for 15 min subsequently take up 32Pi from an external medium only to about 5% of the extent of uptake by control mitochondria. 32pi 110-114 nucleus accumbens associated 1 Rattus norvegicus 57-61 1098-10 1976 When (Ca2+ plus Mg2+)-ATPase activity was measured by 32Pi release from (gamma-32P)ATP, freeze-thawed erythrocytes, as well as membranes prepared at pH 5.8 and at pH 7.6, expressed lower values than noted by assay for total Pi release. 32pi 54-58 dynein axonemal heavy chain 8 Homo sapiens 22-28 187955-0 1976 Incorporation of 32Pi into pyruvate dehydrogenase phosphate in mitochondria from control and insulin-treated adipose tissue. 32pi 17-21 insulin Homo sapiens 93-100 177416-6 1976 The purified enzyme was reconstituted with phospholipids, coupling factor 1 (F1), and the oligomycin sensitivity-conferring protein (OSCP) yielding vesicles with highly active 32Pi-ATP exchange (up to 260 nanomoles/min/mg at 30 degrees), and a proton pump driven by ATP. 32pi 176-180 ATP synthase peripheral stalk subunit OSCP Bos taurus 90-131 177416-6 1976 The purified enzyme was reconstituted with phospholipids, coupling factor 1 (F1), and the oligomycin sensitivity-conferring protein (OSCP) yielding vesicles with highly active 32Pi-ATP exchange (up to 260 nanomoles/min/mg at 30 degrees), and a proton pump driven by ATP. 32pi 176-180 ATP synthase peripheral stalk subunit OSCP Bos taurus 133-137 812702-1 1976 The incorporation of 32Pi into ATP has been found to be catalyzed by myosin only when and if it interacts with actin. 32pi 21-25 myosin heavy chain 14 Homo sapiens 69-75 1212220-13 1975 In a Ca2+-depleted buffer, the uptake of 32Pi is diminished, and hyperosmolarity, which stimulates 45Ca uptake, also accelerates the accumulation of 32Pi. 32pi 149-153 carbonic anhydrase 2 Rattus norvegicus 5-8 172738-1 1975 Myosin catalyzed exchange between 32Pi and ATP in reaction medium during its enzymatic hydrolysis of ATP only by a very small amount. 32pi 34-38 myosin heavy chain 14 Homo sapiens 0-6 29309666-9 2018 In vivo 32Pi labeling revealed that ABA stimulated the formation of PIP2 in germinating seeds and guard cell-enriched leaf peels, which was significantly reduced in plc3 mutants. 32pi 8-12 plasma membrane intrinsic protein 2A Arabidopsis thaliana 68-72 31178874-6 2019 Using a differential 32Pi-labeling strategy combined with transphosphatidylation assays and T-DNA insertion mutants, we found that phospholipase D (PLD), and in particular PLDdelta was the main contributor of the increase in PA. 32pi 21-25 phospholipase D alpha 1 Arabidopsis thaliana 131-146 31178874-6 2019 Using a differential 32Pi-labeling strategy combined with transphosphatidylation assays and T-DNA insertion mutants, we found that phospholipase D (PLD), and in particular PLDdelta was the main contributor of the increase in PA. 32pi 21-25 phospholipase D alpha 1 Arabidopsis thaliana 148-151 30542361-12 2018 In vivo 32Pi-labeling of seedlings and treatment with sorbitol to mimic drought stress, revealed stronger PIP2 responses in both drought-tolerant plc5/7 and PLC7-OE mutants. 32pi 8-12 plasma membrane intrinsic protein 2A Arabidopsis thaliana 106-110 29309666-9 2018 In vivo 32Pi labeling revealed that ABA stimulated the formation of PIP2 in germinating seeds and guard cell-enriched leaf peels, which was significantly reduced in plc3 mutants. 32pi 8-12 phospholipase C1 Arabidopsis thaliana 165-169 25301276-9 2015 GSK3beta also increases the incorporation of 32Pi into GlyT1 and decreases that of GlyT2. 32pi 45-49 glycogen synthase kinase 3 beta L homeolog Xenopus laevis 0-8 25301276-9 2015 GSK3beta also increases the incorporation of 32Pi into GlyT1 and decreases that of GlyT2. 32pi 45-49 solute carrier family 6 member 9 L homeolog Xenopus laevis 55-60