PMID-sentid Pub_year Sent_text compound_name comp_offset prot_official_name organism prot_offset 33668885-11 2021 Furthermore, inhibition of lysosomal hyperpolarization with Bafilomycin A1 blocked LMP and NLRP3 activation, suggesting a causal relation between lysosomal hyperpolarization and LMP. bafilomycin A1 60-74 NLR family, pyrin domain containing 3 Mus musculus 91-96 34035861-9 2021 The number of GFP-LC3 puncta in HCs following exposure to HDN was increased compared with that observed in HCs without HDN exposure under the H/R conditions after bafilomycin A1 treatment. bafilomycin A1 163-177 microtubule associated protein 1 light chain 3 alpha Homo sapiens 18-21 33145969-0 2021 Bafilomycin A1 enhances NLRP3 inflammasome activation in human monocytes independent of lysosomal acidification. bafilomycin A1 0-14 NLR family pyrin domain containing 3 Homo sapiens 24-29 33145969-3 2021 Here we report that pharmacological inhibition of V-ATPase with bafilomycin A1 exacerbated LPS-induced NLRP3 inflammasome activation in primary human monocytes. bafilomycin A1 64-78 NLR family pyrin domain containing 3 Homo sapiens 103-108 32724177-10 2021 Cotreatment with the autophagy inhibitor 3-MA or bafilomycin A1 abrogated the in vivo and in vitro neuroprotective effects of GM1 ganglioside. bafilomycin A1 49-63 coenzyme Q10A Mus musculus 126-129 34048091-5 2021 Pre-incubation of retina with bafilomycin A1 (an inhibitor of neurotransmitter uptake into synaptic vesicles) abolished NMDAR-mediated noise in AII, but not A17 amacrines, suggesting a vesicular source of glutamate activates AII NMDARs, whereas a non-vesicular source activates A17 NMDARs. bafilomycin A1 30-44 NLR family pyrin domain containing 3 Homo sapiens 144-147 34048091-5 2021 Pre-incubation of retina with bafilomycin A1 (an inhibitor of neurotransmitter uptake into synaptic vesicles) abolished NMDAR-mediated noise in AII, but not A17 amacrines, suggesting a vesicular source of glutamate activates AII NMDARs, whereas a non-vesicular source activates A17 NMDARs. bafilomycin A1 30-44 NLR family pyrin domain containing 3 Homo sapiens 225-228 34048091-5 2021 Pre-incubation of retina with bafilomycin A1 (an inhibitor of neurotransmitter uptake into synaptic vesicles) abolished NMDAR-mediated noise in AII, but not A17 amacrines, suggesting a vesicular source of glutamate activates AII NMDARs, whereas a non-vesicular source activates A17 NMDARs. bafilomycin A1 30-44 immunoglobulin kappa variable 2-30 Homo sapiens 278-281 33749503-4 2021 Our data revealed that LC3-II formation and SQSTM1/p62 degradation were noted in H2O2-activated human platelets, which could be blocked by 3-methyladenine and bafilomycin A1, indicating that platelet activation may cause platelet autophagy. bafilomycin A1 159-173 microtubule associated protein 1 light chain 3 alpha Homo sapiens 23-26 33749503-4 2021 Our data revealed that LC3-II formation and SQSTM1/p62 degradation were noted in H2O2-activated human platelets, which could be blocked by 3-methyladenine and bafilomycin A1, indicating that platelet activation may cause platelet autophagy. bafilomycin A1 159-173 sequestosome 1 Homo sapiens 44-50 33749503-4 2021 Our data revealed that LC3-II formation and SQSTM1/p62 degradation were noted in H2O2-activated human platelets, which could be blocked by 3-methyladenine and bafilomycin A1, indicating that platelet activation may cause platelet autophagy. bafilomycin A1 159-173 sequestosome 1 Homo sapiens 51-54 33869205-9 2021 Inhibiting miR-22 promoted autophagic flux confirmed by an increased LC3II/GAPDH ratio and reduced p62 protein level under bafilomycin A1 conditions. bafilomycin A1 123-137 microRNA 22 Mus musculus 11-17 33727633-8 2021 The mRNA levels of AE2 in each KO cell did not change, and the downregulation of AE2 in L-IRBIT KO cells was inhibited by bafilomycin A1. bafilomycin A1 122-136 solute carrier family 4 member 2 Homo sapiens 81-84 33727633-8 2021 The mRNA levels of AE2 in each KO cell did not change, and the downregulation of AE2 in L-IRBIT KO cells was inhibited by bafilomycin A1. bafilomycin A1 122-136 adenosylhomocysteinase like 1 Homo sapiens 90-95 33746976-5 2021 The use of pharmacological agents, rapamycin and bafilomycin A1, reveals that RipA activates PI3K-AKT-mTORC1 signaling cascade that ultimately culminates in the inhibition of autophagy initiating kinase ULK1 (Unc-51 like autophagy activating kinase). bafilomycin A1 49-63 CREB regulated transcription coactivator 1 Mus musculus 102-108 33639976-10 2021 Moreover, in the hACE2 transgenic mice model of SARS-CoV-2 infection, chloroquine and bafilomycin A1 reduced viral replication in lung tissues and alleviated viral pneumonia with reduced inflammatory exudation and infiltration in peribronchiolar and perivascular tissues, as well as improved structures of alveolar septum and pulmonary alveoli. bafilomycin A1 86-100 angiotensin converting enzyme 2 Homo sapiens 17-22 33421769-5 2021 The lysosome inhibitor bafilomycin A1 or deletion of nuclear receptor coactivator 4 (NCOA4) inhibited cystine deprivation-induced decrease in FTH1 protein levels and cell death. bafilomycin A1 23-37 ferritin heavy chain 1 Homo sapiens 142-146 33668422-4 2021 In vitro experiments using mouse and human cardiac fibroblasts confirmed that blockade of autophagy with Bafilomycin A1 inhibited fibroblast-to-myofibroblast transition induced by transforming growth factor (TGF)-beta. bafilomycin A1 105-119 transforming growth factor alpha Homo sapiens 180-217 33384144-6 2021 We find that when 14 was co-administrated with Bafilomycin A1 (BAF) in PC3 cells, rapid necrotic cell death occurred with no cleaved caspase-3 and cleaved PARP activation and increasing the expression of survivin. bafilomycin A1 47-61 poly(ADP-ribose) polymerase 1 Homo sapiens 155-159 33486901-11 2021 So, the cumulative effect of oncogenic p62 should be considered when using autophagy inhibitors, especially drugs that act at the last stage of autophagy such as chloroquine and bafilomycin A1. bafilomycin A1 178-192 nucleoporin 62 Homo sapiens 39-42 33285240-7 2021 The lysosome inhibitor bafilomycin A1 increased xCT protein levels in the absence of EGF or in the presence of EGF and Torin 1. bafilomycin A1 23-37 solute carrier family 7 member 11 Homo sapiens 48-51 33526785-10 2021 Inhibition of autophagy using bafilomycin A1 or Beclin1 siRNA leads to reversal of Neu2-induced apoptosis suggesting their possible relationship. bafilomycin A1 30-44 neuraminidase 2 Homo sapiens 83-87 33526785-13 2021 Such changes were absent in the presence of bafilomycin A1 indicating the involvement of autophagy in Neu2-induced anoikis. bafilomycin A1 44-58 neuraminidase 2 Homo sapiens 102-106 33676894-5 2021 Overexpression of Vps24, a protein that sorts transmembrane proteins into lysosomes via the multivesicular body (MVB) pathway, accelerated whereas the lysosome inhibitor bafilomycin A1 completely prevented PKC-mediated Kv1.5 degradation. bafilomycin A1 170-184 potassium voltage-gated channel subfamily A member 5 Homo sapiens 219-224 33459130-4 2021 Accumulation of LC3-II in elicited atg7-/- macrophages in response to bafilomycin A1 validated these data. bafilomycin A1 70-84 Atg7p Saccharomyces cerevisiae S288C 35-39 33552025-10 2020 Consistent with this mechanism, inhibition of autophagolysosome maturation with bafilomycin A1 abrogated the ability of THP-PKR macrophages to limit replication of Mtb, whereas pharmacological activation of autophagy enhanced the anti-mycobacterial effect of PKR overexpression. bafilomycin A1 80-94 eukaryotic translation initiation factor 2 alpha kinase 2 Homo sapiens 124-127 32950809-7 2020 Mechanistically, bafilomycin A1 and Atg5 small interfering RNA were used to evaluate the possible mechanism of autophagy activated by irisin, and it was identified that irisin may upregulate autophagy by increasing the Atg12-Atg5-Atg16L complex. bafilomycin A1 17-31 fibronectin type III domain containing 5 Homo sapiens 169-175 33308286-11 2020 Additionally, bafilomycin A1, an autophagy inhibitor, reversed the ATP13A2-induced stemness of colon cancer cells. bafilomycin A1 14-28 ATPase cation transporting 13A2 Homo sapiens 67-74 33292101-7 2020 Bioinformatics analysis of GEO data, GSE60570 accession number, revealed that p38 signalling induction and DNA damage response are among the main disrupted signalling pathways in bafilomycin A1-treated RPE-1 cells. bafilomycin A1 179-193 mitogen-activated protein kinase 14 Homo sapiens 78-81 33453994-7 2020 Consistent with high-dose liproxstatin-1 protecting via a lysosomal mechanism, we further de-monstrated that loss of viability induced by alpha-synuclein PFFs was attenuated by chloroquine and bafilomycin A1 as well as the lysosomal cysteine protease inhibitors, leupeptin, E-64D, and Ca-074-Me, but not other autophagy or lysosomal enzyme inhibitors. bafilomycin A1 193-207 synuclein, alpha Mus musculus 138-153 33201170-4 2020 This process is blocked by bafilomycin A1 that binds and inhibits the vacuolar ATPase (V-ATPase) and by SopF, a bacterial effector that catalytically modifies the V-ATPase to inhibit LC3B lipidation via ATG16L1. bafilomycin A1 27-41 microtubule associated protein 1 light chain 3 beta Homo sapiens 183-187 33201170-4 2020 This process is blocked by bafilomycin A1 that binds and inhibits the vacuolar ATPase (V-ATPase) and by SopF, a bacterial effector that catalytically modifies the V-ATPase to inhibit LC3B lipidation via ATG16L1. bafilomycin A1 27-41 autophagy related 16 like 1 Homo sapiens 203-210 32736005-9 2020 Bafilomycin A1 almost completely prevented the generation of cytoplasmic vacuoles and significantly attenuated cytotoxicity induced by maduramicin, along with downregulation of K-Ras and upregulation of Rac1. bafilomycin A1 0-14 KRAS proto-oncogene, GTPase Gallus gallus 177-182 32810509-10 2020 Using LB-100, a PP2A inhibitor, AICAR, an agonist of AMPK, and bafilomycin A1 (Baf-A1), an autophagy inhibitor, we discover that FTY720 induces ferroptosis and autophagy through the PP2A/AMPK pathway, and ferroptosis and autophagy can reinforce each other. bafilomycin A1 63-77 protein phosphatase 2 phosphatase activator Homo sapiens 182-186 32810509-10 2020 Using LB-100, a PP2A inhibitor, AICAR, an agonist of AMPK, and bafilomycin A1 (Baf-A1), an autophagy inhibitor, we discover that FTY720 induces ferroptosis and autophagy through the PP2A/AMPK pathway, and ferroptosis and autophagy can reinforce each other. bafilomycin A1 63-77 protein kinase AMP-activated catalytic subunit alpha 2 Homo sapiens 187-191 32792197-6 2020 In addition, we found that treatment with ursolic acid promotes melanosomal degradation, and bafilomycin A1 inhibition of autophagosome-lysosome fusion blocked the removal of melanosomes in alpha-MSH-stimulated B16F1 cells. bafilomycin A1 93-107 STAM binding protein Mus musculus 190-199 32736005-9 2020 Bafilomycin A1 almost completely prevented the generation of cytoplasmic vacuoles and significantly attenuated cytotoxicity induced by maduramicin, along with downregulation of K-Ras and upregulation of Rac1. bafilomycin A1 0-14 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) Gallus gallus 203-207 32693673-8 2020 Moreover, the LAPTM4B-overexpression-mediated benefits were abolished by knockdown of lysosome-associated membrane protein-2 (an autophagosome-lysosome fusion protein) in vitro and by the autophagy inhibitor bafilomycin A1 in vivo. bafilomycin A1 208-222 lysosomal-associated protein transmembrane 4B Mus musculus 14-21 32976350-0 2020 Phagocytosis of Bafilomycin A1-treated Apoptotic Neuroblastoma Cells by Bone Marrow-derived Dendritic Cells Initiates a CD8alpha+ Lymphocyte Response to Neuroblastoma: Erratum. bafilomycin A1 16-30 CD8a molecule Homo sapiens 120-128 33041800-8 2020 When in the presence of bafilomycin A1 (BAF), the Ubc9 adenovirus plus OGD group presented a higher level of LC3 II and GFP-LC3 puncta than the OGD group. bafilomycin A1 24-38 ubiquitin-conjugating enzyme E2I Mus musculus 50-54 33041800-8 2020 When in the presence of bafilomycin A1 (BAF), the Ubc9 adenovirus plus OGD group presented a higher level of LC3 II and GFP-LC3 puncta than the OGD group. bafilomycin A1 40-43 ubiquitin-conjugating enzyme E2I Mus musculus 50-54 33029288-4 2020 The role of autophagy and AMP-activated protein kinase (AMPK) was determined by treating NSCLC cells with bafilomycin A1, an autophagy inhibitor, and compound C, an AMPK inhibitor. bafilomycin A1 106-120 protein kinase AMP-activated catalytic subunit alpha 1 Homo sapiens 56-60 33029288-7 2020 Treatment with bafilomycin A1 markedly reduced cell viability but increased proportions of sub-G1 phase cells and annexin V-positive cells in H460 cells. bafilomycin A1 15-29 annexin A5 Homo sapiens 114-123 32943718-6 2020 Rubicon overexpression or administration of bafilomycin A1 or chloroquine, an inhibitor of late-stage autophagy, suppressed autophagy and activated the type 1 IFN pathway. bafilomycin A1 44-58 interferon alpha 1 Homo sapiens 159-162 32469087-7 2020 Concurrently, HMGB1 impaired insulin sensitivities by attenuating the abundance of insulin receptor substrate-1, Akt phosphorylation, GLUT4 translocation, and glucose uptake in granulosa cells, which were reversed by blocking autophagy pathways with siRNA-mediated knockdown of ATG7 or with chloroquine and bafilomycin A1, the lysosome inhibitors. bafilomycin A1 307-321 high mobility group box 1 Homo sapiens 14-19 32708051-10 2020 The autophagy inhibitor bafilomycin A1, but not the proteasome inhibitor MG132, inhibited the beta-cryptoxanthin-induced decrease in p62 and LC3-II expressions. bafilomycin A1 24-38 nucleoporin 62 Mus musculus 133-136 32714200-8 2020 Similarly, pretreatment of HEK293 cells with either the lysosomal inhibitor bafilomycin A1 or the proteasomal inhibitor MG132 reversed the inhibitory effects of SPAK knockdown on BK expression. bafilomycin A1 76-90 serine/threonine kinase 39 Homo sapiens 161-165 32463725-10 2020 Similarly, pretreatment of the lysosomal inhibitor, bafilomycin A1 reversed the inhibitory effects of 14-3-3gamma on BK protein expression. bafilomycin A1 52-66 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma Homo sapiens 102-113 32453975-4 2020 Consistently, melatonin treatment during in vitro maturation also attenuated the meiotic defects induced by H2O2 by regulating autophagy and SIRT1, which could be abolished by SIRT1 inhibitor, Ex527 and autophagy inhibitor Bafilomycin A1 (Baf A1). bafilomycin A1 223-237 sirtuin 1 Mus musculus 176-181 32685103-11 2020 In addition, both bafilomycin A1 (a lysosome inhibitor) and an AMPK inhibitor (compound c) markedly prevented salidroside-induced autophagic degradation of p-Src and caveolin-1. bafilomycin A1 18-32 Rous sarcoma oncogene Mus musculus 158-161 32685103-11 2020 In addition, both bafilomycin A1 (a lysosome inhibitor) and an AMPK inhibitor (compound c) markedly prevented salidroside-induced autophagic degradation of p-Src and caveolin-1. bafilomycin A1 18-32 caveolin 1, caveolae protein Mus musculus 166-176 32209654-4 2020 We found that administration of autophagic inhibitors such as chloroquine or bafilomycin A1, or depletion of autophagy-related 5 (ATG5), results in accumulation of intracellular VEGFA, indicating that VEGFA is a basal autophagic substrate. bafilomycin A1 77-91 vascular endothelial growth factor A Mus musculus 178-183 32549199-3 2020 We have shown that silencing of PKCepsilon by siRNA inhibited basal and starvation-induced autophagy in T47D breast cancer cells as determined by the decrease in LC3-II, increase in p62, and decrease in autophagy puncta both in the presence and absence of bafilomycin A1. bafilomycin A1 256-270 protein kinase C epsilon Homo sapiens 32-42 32265299-8 2020 The lysosome inhibitor bafilomycin A1 suppressed xCT down-regulation in HD-cultured glioblastoma cells and in Torin 1-treated cells grown at low density. bafilomycin A1 23-37 solute carrier family 7 member 11 Homo sapiens 49-52 32626542-12 2020 We found that after treatment with proteasomal inhibitor MG132 and lysosomal inhibitor bafilomycin A1, MDR3 expression of V399L was significantly increased. bafilomycin A1 87-101 ATP binding cassette subfamily B member 4 Homo sapiens 103-107 32397873-3 2021 CASP9-deficient cells demonstrate the missorting of CTSD (cathepsin D) and other acid hydrolases, accumulation of late endosomes, and reduced degradation of bafilomycin A1-sensitive proteins. bafilomycin A1 157-171 caspase 9 Homo sapiens 0-5 32414129-4 2020 Inhibition of autophagy by a chemical agent (such as chloroquine, bafilomycin A1, or 3-methyladenine) increases the AHR protein levels in HeLa cells whereas activation of autophagy by short-term nutrition deprivation reduces its levels. bafilomycin A1 66-80 aryl hydrocarbon receptor Homo sapiens 116-119 32209654-4 2020 We found that administration of autophagic inhibitors such as chloroquine or bafilomycin A1, or depletion of autophagy-related 5 (ATG5), results in accumulation of intracellular VEGFA, indicating that VEGFA is a basal autophagic substrate. bafilomycin A1 77-91 vascular endothelial growth factor A Mus musculus 201-206 32273718-12 2020 The autophagy inhibitor Bafilomycin A1 (BafA1) repressed both autophagy and apoptosis, indicating that miR-126 induced autophagy was responsible for the induction of apoptosis. bafilomycin A1 24-38 microRNA 126 Homo sapiens 103-110 32248306-8 2020 The protective effect of CARD9 on cardiomyocytes against H/R-induced oxidative stress was abolished by treatment with autophagy inhibitors, 3-methyladenine (3-MA) or Bafilomycin A1(BafA1). bafilomycin A1 166-180 caspase recruitment domain family, member 9 Mus musculus 25-30 32273718-12 2020 The autophagy inhibitor Bafilomycin A1 (BafA1) repressed both autophagy and apoptosis, indicating that miR-126 induced autophagy was responsible for the induction of apoptosis. bafilomycin A1 40-45 microRNA 126 Homo sapiens 103-110 30621446-5 2020 Importantly, the protective effects of isopsoralen against IL-1beta were blocked by pretreatment with autophagy inhibitor 3-MA and bafilomycin A1. bafilomycin A1 131-145 interleukin 1 beta Rattus norvegicus 59-67 31902824-7 2020 The expression levels of CD81 in HEK293T cells treated with a proteasome inhibitor (lactacystin) and lysosome inhibitors (chloroquine and bafilomycin A1) were analyzed by flow cytometry. bafilomycin A1 138-152 CD81 molecule Homo sapiens 25-29 31836471-8 2020 Further, alpha-Synuclein mRNA was decreased by the inhibition of autophagy by using inhibitor bafilomycin A1 or by silencing Atg5 with siRNA. bafilomycin A1 94-108 synuclein, alpha Mus musculus 9-24 31544984-12 2020 The late autophagy inhibitor bafilomycin A1 (Baf-A1) and the Wnt/beta-catenin pathway inhibitor PKF115-584 reversed the effects of LRP6 on trophoblast autophagy, migration and invasion. bafilomycin A1 29-43 LDL receptor related protein 6 Homo sapiens 131-135 31544984-12 2020 The late autophagy inhibitor bafilomycin A1 (Baf-A1) and the Wnt/beta-catenin pathway inhibitor PKF115-584 reversed the effects of LRP6 on trophoblast autophagy, migration and invasion. bafilomycin A1 45-51 LDL receptor related protein 6 Homo sapiens 131-135 31952347-5 2020 The proteasome inhibitor MG132 and the autophagy inhibitor bafilomycin A1 were found to upregulate the PDCD4 levels. bafilomycin A1 59-73 programmed cell death 4 Homo sapiens 103-108 31794745-7 2020 Whereas inhibition of degradation of autolysosomes by bafilomycin A1 (Baf-A1) reversed functional recovery caused by Tubastatin A, revealing the association between tubulin acetylation and autophagy, which supports HDAC6 inhibition as a potential target for SCI treatment. bafilomycin A1 70-76 histone deacetylase 6 Mus musculus 215-220 31696568-6 2020 In contrast, inactivation of autophagy by 3-methyladenine or Bafilomycin A1 could attenuate OPG-mediated inhibition of OC differentiation via the AMPK/mTOR/p70S6K signalling pathway. bafilomycin A1 61-75 TNF receptor superfamily member 11b Homo sapiens 92-95 31696568-6 2020 In contrast, inactivation of autophagy by 3-methyladenine or Bafilomycin A1 could attenuate OPG-mediated inhibition of OC differentiation via the AMPK/mTOR/p70S6K signalling pathway. bafilomycin A1 61-75 protein kinase AMP-activated catalytic subunit alpha 2 Homo sapiens 146-150 31889041-8 2019 Therefore, whether melatonin regulates the amylin expression/oligomerization in thapsigargin- or tunicamycin-combined with Bafilomycin A1 (autophagy inhibitor) or MG132 (proteasome inhibitor) treatments were investigated. bafilomycin A1 123-137 islet amyloid polypeptide Rattus norvegicus 43-49 31707034-12 2019 The efficacy of CA on A53T alpha-synuclein degradation was reversed by the autophagy inhibitor bafilomycin A1 and the JNK inhibitor SP600125. bafilomycin A1 95-109 synuclein, alpha Mus musculus 27-42 31960608-10 2020 The addition of autophagy inhibitors 3-methyladenine (10 mM) or bafilomycin A1 (10 muM) reduced caffeine-dependent lipid utilization by approximately 30%. bafilomycin A1 64-78 latexin Homo sapiens 83-86 31541377-7 2019 Mn-induced degradation of ZIP14 was blocked by bafilomycin A1, which increased localization of the transporter in Lamp1-positive vesicles. bafilomycin A1 47-61 solute carrier family 39 member 14 Homo sapiens 26-31 31541377-7 2019 Mn-induced degradation of ZIP14 was blocked by bafilomycin A1, which increased localization of the transporter in Lamp1-positive vesicles. bafilomycin A1 47-61 lysosomal associated membrane protein 1 Homo sapiens 114-119 31889041-9 2019 Amylin expression/oligomerization with melatonin treatment was significantly decreased in the thapsigargin- or tunicamycin-combined Bafilomycin A1 or MG132 treatments. bafilomycin A1 132-146 islet amyloid polypeptide Rattus norvegicus 0-6 31118030-7 2019 Confocal microscopic data indicated a synergism of NaIO3 and bafilomycin A1 on LC3 punctate formation, indicating the induction of autophagy. bafilomycin A1 61-75 microtubule associated protein 1 light chain 3 alpha Homo sapiens 79-82 31332960-9 2019 The protein levels of LC3-II and p62 were increased in cells overexpression of wild-type and mutant WDR45 while the protein levels were not increased in cells overexpression of mutant WDR45 upon treatment with autophagy inhibitor Bafilomycin A1. bafilomycin A1 230-244 microtubule associated protein 1 light chain 3 alpha Homo sapiens 22-25 31332960-9 2019 The protein levels of LC3-II and p62 were increased in cells overexpression of wild-type and mutant WDR45 while the protein levels were not increased in cells overexpression of mutant WDR45 upon treatment with autophagy inhibitor Bafilomycin A1. bafilomycin A1 230-244 WD repeat domain 45 Homo sapiens 184-189 31332960-10 2019 Results from confocal microscopy revealed that LC3-positive puncta were increased in cells expressing both wild-type and mutant WDR45 while the number of LC3-positive puncta was not increased in cells expressing mutant WDR45 upon treatment with Bafilomycin A1. bafilomycin A1 245-259 microtubule associated protein 1 light chain 3 alpha Homo sapiens 47-50 31332960-10 2019 Results from confocal microscopy revealed that LC3-positive puncta were increased in cells expressing both wild-type and mutant WDR45 while the number of LC3-positive puncta was not increased in cells expressing mutant WDR45 upon treatment with Bafilomycin A1. bafilomycin A1 245-259 WD repeat domain 45 Homo sapiens 219-224 31657331-16 2019 In Baf+LPS group, PD-L1 mRNA expression was significantly up-regulated as compared with that in LPS stimulation group [12-hour PD-L1 mRNA (2-DeltaDeltaCT): 109.1+-7.4 vs. 72.2+-10.0, P < 0.01]. bafilomycin A1 3-6 CD274 antigen Mus musculus 18-23 31657331-16 2019 In Baf+LPS group, PD-L1 mRNA expression was significantly up-regulated as compared with that in LPS stimulation group [12-hour PD-L1 mRNA (2-DeltaDeltaCT): 109.1+-7.4 vs. 72.2+-10.0, P < 0.01]. bafilomycin A1 3-6 CD274 antigen Mus musculus 127-132 31657331-19 2019 On the contrary, the expressions of PD-L1, LC3 and p62 in the Baf+LPS group were significantly up-regulated, indicating that the binding of autophagy and lysosome was blocked, and autophagy was not smooth. bafilomycin A1 62-65 CD274 antigen Mus musculus 36-41 31657331-19 2019 On the contrary, the expressions of PD-L1, LC3 and p62 in the Baf+LPS group were significantly up-regulated, indicating that the binding of autophagy and lysosome was blocked, and autophagy was not smooth. bafilomycin A1 62-65 microtubule-associated protein 1 light chain 3 alpha Mus musculus 43-46 31657331-19 2019 On the contrary, the expressions of PD-L1, LC3 and p62 in the Baf+LPS group were significantly up-regulated, indicating that the binding of autophagy and lysosome was blocked, and autophagy was not smooth. bafilomycin A1 62-65 nucleoporin 62 Mus musculus 51-54 30909132-6 2019 Primary HNECs were treated with HNE, bafilomycin A1, and SP600125. bafilomycin A1 37-51 elastase, neutrophil expressed Homo sapiens 8-11 31116586-6 2019 Inhibiting mTORC1 with everolimus rapidly increased cyclin D1 degradation, whereas 3-methyladenine, chloroquine, and bafilomycin A1, the classic autophagy inhibitors, could attenuate everolimus-induced cyclin D1 degradation. bafilomycin A1 117-131 cyclin D1 Homo sapiens 202-211 30924217-11 2019 MR formation and ROS production were significantly inhibited in thoracic aorta from AngII-induced rats treated with bafilomycin A1 and amitriptyline. bafilomycin A1 116-130 angiotensinogen Rattus norvegicus 84-89 31327962-8 2019 Next, the drug bafilomycin A1 (BAF, 50 nM) was used to block the autophagic flux. bafilomycin A1 15-29 BAF nuclear assembly factor 1 Homo sapiens 31-34 31108937-5 2019 Comparing the fluorescence characteristics of these proteins in response to intracellular pH changes induced by chloroquine or bafilomycin A1, we found that pHluorin-BACE1-mCherry is a better pH sensor for BACE1 because its fluorescence intensity responds to pH changes more dramatically and more quickly. bafilomycin A1 127-141 beta-secretase 1 Homo sapiens 166-171 31108937-5 2019 Comparing the fluorescence characteristics of these proteins in response to intracellular pH changes induced by chloroquine or bafilomycin A1, we found that pHluorin-BACE1-mCherry is a better pH sensor for BACE1 because its fluorescence intensity responds to pH changes more dramatically and more quickly. bafilomycin A1 127-141 beta-secretase 1 Homo sapiens 206-211 30805671-8 2019 Moreover, inhibiting A2AR by antagonist (SCH 58261) performed the same downstream biological effects as caffeine treatment, and autophagy inhibitor (BafilomycinA1) clearly abolished the caffeine-induced Bcl10 degradation and A20 suppression. bafilomycin A1 149-162 B cell leukemia/lymphoma 10 Mus musculus 203-208 30805671-8 2019 Moreover, inhibiting A2AR by antagonist (SCH 58261) performed the same downstream biological effects as caffeine treatment, and autophagy inhibitor (BafilomycinA1) clearly abolished the caffeine-induced Bcl10 degradation and A20 suppression. bafilomycin A1 149-162 tumor necrosis factor, alpha-induced protein 3 Mus musculus 225-228 30686534-5 2019 In contrast, genetic ablation (using ATG5-/- or ATG7-/- cells) or pharmacologic inhibition (the administration of bafilomycin A1 or chloroquine) of autophagy was found to block ferroptosis activator-induced HMGB1 release. bafilomycin A1 114-128 high mobility group box 1 Homo sapiens 207-212 30789754-6 2019 Also, the inhibition of the lysosomal pathway with bafilomycin A1 (80 nM, 24 h) resulted in a significant increase in NPC1L1 protein levels. bafilomycin A1 51-65 NPC1 like intracellular cholesterol transporter 1 Homo sapiens 118-124 30893306-8 2019 Bafilomycin A1, a specific inhibitor of vacuolar-type proton ATPase (V-ATPase) which alkalizes acidic vesicles, abolished ZnT2-dependent zinc transport into intracellular vesicles. bafilomycin A1 0-14 solute carrier family 30 member 2 Homo sapiens 122-126 30587795-6 2018 Morphologically, LT-IIc induced the formation of enlarged LAMP2+ autolysosomes, which was blocked by co-treatment with bafilomycin A1. bafilomycin A1 119-133 lysosomal associated membrane protein 2 Homo sapiens 58-63 30557043-6 2019 Inhibitors of bone resorption, such as alendronate, bafilomycin A1, and the PI3K inhibitor LY294002, suppressed the expression of Pmepa1 in osteoclasts. bafilomycin A1 52-66 prostate transmembrane protein, androgen induced 1 Rattus norvegicus 130-136 30553838-9 2019 Autophagy was inhibited in ALR-knockdown mice and HepG2 cells, and autophagy inhibitor bafilomycin A1 attenuated the protective effects of ALR. bafilomycin A1 87-101 growth factor, augmenter of liver regeneration Homo sapiens 139-142 29486589-10 2019 Moreover, blocking autophagy with bafilomycin A1 or LC3B siRNA resulted in oxidant accumulation, NLRP3 inflammasome hyperactivation, and collagen deposition. bafilomycin A1 34-48 NLR family pyrin domain containing 3 Homo sapiens 97-102 30584497-6 2018 Anti-HRV1B activity of DEX via the glucocorticoid receptor (GCR)-dependent autophagy activation was assessed by blocking with chloroquine diphosphate salt or bafilomycin A1 treatment. bafilomycin A1 158-172 nuclear receptor subfamily 3 group C member 1 Homo sapiens 60-63 29807114-3 2018 Further, SFN-NAC increased LC3 II/LC3 I and the number of LC3 punctas, but Western blot showed that SFN-NAC inhibited cell autophagy in response to a co-treatment of Bafilomycin A1 and SFN-NAC. bafilomycin A1 166-180 RNA exonuclease 2 Homo sapiens 100-103 30282964-8 2018 The protective effects of TRPC6 ablation were prevented by autophagy inhibitors Chloroquine and Bafilomycin A1. bafilomycin A1 96-110 transient receptor potential cation channel subfamily C member 6 Homo sapiens 26-31 29767559-8 2018 Pretreatments of Cos-7 cells with either the lysosomal inhibitor bafilomycin A1 or the proteasomal inhibitor MG132 partially reversed the inhibitory effects of GPS2 siRNA on BK protein expression. bafilomycin A1 65-79 G protein pathway suppressor 2 Mus musculus 160-164 29807114-3 2018 Further, SFN-NAC increased LC3 II/LC3 I and the number of LC3 punctas, but Western blot showed that SFN-NAC inhibited cell autophagy in response to a co-treatment of Bafilomycin A1 and SFN-NAC. bafilomycin A1 166-180 synuclein alpha Homo sapiens 104-107 29807114-3 2018 Further, SFN-NAC increased LC3 II/LC3 I and the number of LC3 punctas, but Western blot showed that SFN-NAC inhibited cell autophagy in response to a co-treatment of Bafilomycin A1 and SFN-NAC. bafilomycin A1 166-180 RNA exonuclease 2 Homo sapiens 100-103 29807114-3 2018 Further, SFN-NAC increased LC3 II/LC3 I and the number of LC3 punctas, but Western blot showed that SFN-NAC inhibited cell autophagy in response to a co-treatment of Bafilomycin A1 and SFN-NAC. bafilomycin A1 166-180 synuclein alpha Homo sapiens 104-107 29146131-10 2018 Furthermore, depigmentation in TSC2-KD melanocytes was accelerated by inhibiting autophagy (ATG7-KD or bafilomycin A1-pretreatment) and was completely reversed by induction of autophagy via mTOR-dependent (rapamycin) or mTOR-independent (SMER28) exposure. bafilomycin A1 103-117 TSC complex subunit 2 Homo sapiens 31-35 29434757-8 2018 Autophagy flux analyzed by microtubule-associated protein 1 light chain 3 (LC3) turnover indicated that miR-221 reduced autophagy in CRC cells using different protease inhibitors (E64d and pepstatin A; Bafilomycin A1) in nutrient-rich medium or under starvation conditions. bafilomycin A1 202-216 microRNA 221 Homo sapiens 104-111 29492987-7 2018 Preventing lysosome damage with bafilomycin A1 or CA-074-Me also counteracted SPION-induced IL-1beta release. bafilomycin A1 32-46 interleukin 1 alpha Mus musculus 92-100 30138927-6 2018 METHODS: NLRP3 inflammasomes were activated in human RPE cells by blocking proteasomes and autophagy using MG-132 and bafilomycin A1 (BafA), respectively. bafilomycin A1 134-138 NLR family pyrin domain containing 3 Homo sapiens 9-14 28840510-6 2018 3-Methyl adenine did not change LC3 conversion; vinblastine and bafilomycin A1 decreased LAMP 2 expression in controls. bafilomycin A1 64-78 lysosomal-associated membrane protein 2 Rattus norvegicus 89-95 28840510-7 2018 While cypermethrin increased LC3 conversion in the presence of 3-methyl adenine, LAMP 2 reduction was more pronounced in vinblastine and bafilomycin A1-treated cells. bafilomycin A1 137-151 lysosomal-associated membrane protein 2 Rattus norvegicus 81-87 29080748-8 2018 By using NH4Cl and Bafilomycin A1 to inhibit the lysosomal degradative pathway, we found that the reduction of APP induced by SNX7 overexpression was prevented by such inhibition. bafilomycin A1 19-33 sorting nexin 7 Homo sapiens 126-130 29203244-6 2018 In addition, ox-LDL increased LC3-II, Beclin 1, and p62 expressions in HUVECs, while these changes were nullified in the presence of treatment with bafilomycin A1 (BafA1, an inhibit autophagic flux inhibitor). bafilomycin A1 148-162 beclin 1 Homo sapiens 38-46 29203244-6 2018 In addition, ox-LDL increased LC3-II, Beclin 1, and p62 expressions in HUVECs, while these changes were nullified in the presence of treatment with bafilomycin A1 (BafA1, an inhibit autophagic flux inhibitor). bafilomycin A1 148-162 nucleoporin 62 Homo sapiens 52-55 30138927-11 2018 It also prevented IL-1beta release after exposure of primed cells to MG-132 and BafA. bafilomycin A1 80-84 interleukin 1 beta Homo sapiens 18-26 30138927-13 2018 The activity of the lysosomal enzyme, cathepsin B, was reduced by MG-132 and BafA, suggesting that cathepsin B was not playing any role in this phenomenon. bafilomycin A1 77-81 cathepsin B Homo sapiens 38-49 28106298-9 2017 Its role in autophagy was confirmed through decrease in the levels of LC3 II after pretreatment with IP3 R inhibitor and N acetyl cysteine.Moreover, inhibiting as well as silencing IP3 R-induced cell death in MCF-7 cells was attenuated by autophagic inhibitors (Bafilomycin A1 or 3-Methyladeneine). bafilomycin A1 262-276 inositol 1,4,5-trisphosphate receptor type 3 Homo sapiens 101-106 28128446-6 2017 AGE impairs autophagosomes" clearance in p53 negative cells as observed with an autophagosome maturation blocker-bafilomycinA1 treated cells. bafilomycin A1 113-126 tumor protein p53 Homo sapiens 41-44 28106298-9 2017 Its role in autophagy was confirmed through decrease in the levels of LC3 II after pretreatment with IP3 R inhibitor and N acetyl cysteine.Moreover, inhibiting as well as silencing IP3 R-induced cell death in MCF-7 cells was attenuated by autophagic inhibitors (Bafilomycin A1 or 3-Methyladeneine). bafilomycin A1 262-276 inositol 1,4,5-trisphosphate receptor type 3 Homo sapiens 181-186 28214847-8 2017 Although free ubiquitin content was increased, the colocalization of it to CDK1 was markedly decreased in CD38-/- or baf treated CAMs. bafilomycin A1 117-120 cyclin-dependent kinase 1 Mus musculus 75-79 28101201-10 2016 Notably, following treatment of HepG2 cells with the autophagy inhibitor, BA1, the expression of apoptosis-related proteins, including Bax, Bak and p53, were significantly decreased (P<0.05), and cell viability was recovered to a certain extent. bafilomycin A1 74-77 BCL2 associated X, apoptosis regulator Homo sapiens 135-138 28359055-8 2017 Treatment with bafilomycin A1 (an inhibitor of vacuolar H+-ATPase, a late autophagy inhibitor) further increase LC3-II and p62 levels, suggesting that degradation of autophagolysosome could be impaired. bafilomycin A1 15-29 microtubule associated protein 1 light chain 3 alpha Homo sapiens 112-115 28359055-8 2017 Treatment with bafilomycin A1 (an inhibitor of vacuolar H+-ATPase, a late autophagy inhibitor) further increase LC3-II and p62 levels, suggesting that degradation of autophagolysosome could be impaired. bafilomycin A1 15-29 nucleoporin 62 Homo sapiens 123-126 27681126-4 2016 This depletion was independent of proteasomes, as it was not affected by the proteasome inhibitor MG132, but it was suppressed by bafilomycin A1, which led to the association of TRAF6 with autophagosomes. bafilomycin A1 130-144 TNF receptor associated factor 6 Homo sapiens 178-183 28101201-10 2016 Notably, following treatment of HepG2 cells with the autophagy inhibitor, BA1, the expression of apoptosis-related proteins, including Bax, Bak and p53, were significantly decreased (P<0.05), and cell viability was recovered to a certain extent. bafilomycin A1 74-77 BCL2 antagonist/killer 1 Homo sapiens 140-143 28101201-10 2016 Notably, following treatment of HepG2 cells with the autophagy inhibitor, BA1, the expression of apoptosis-related proteins, including Bax, Bak and p53, were significantly decreased (P<0.05), and cell viability was recovered to a certain extent. bafilomycin A1 74-77 tumor protein p53 Homo sapiens 148-151 27397870-9 2016 The formation of CaOx appears to be dependent on the cooperation between Prestin and the vATPase complex as treatment with bafilomycin A1 or concanamycin A abolishes the production of detectable CaOx. bafilomycin A1 123-137 prestin Drosophila melanogaster 73-80 27246695-8 2016 Paraquat-elicited changes in cardiac autophagy markers (LC3BII, LC3BII-to-LC3BI ratio and p62) were augmented by lysosomal inhibition using bafilomycin A1 in WT mice. bafilomycin A1 140-154 nucleoporin 62 Mus musculus 90-93 27513923-4 2016 Studies with Atg5 small interfering RNA (siRNA) and the autophagy inhibitors bafilomycin A1 (Baf) and chloroquine demonstrate that autophagy is required for AGGF1-mediated EC proliferation, migration, capillary tube formation, and aortic ring-based angiogenesis. bafilomycin A1 77-91 angiogenic factor with G patch and FHA domains 1 Mus musculus 157-162 27513923-4 2016 Studies with Atg5 small interfering RNA (siRNA) and the autophagy inhibitors bafilomycin A1 (Baf) and chloroquine demonstrate that autophagy is required for AGGF1-mediated EC proliferation, migration, capillary tube formation, and aortic ring-based angiogenesis. bafilomycin A1 93-96 angiogenic factor with G patch and FHA domains 1 Mus musculus 157-162 27430213-9 2016 Further validation analyses were focused on V-ATPase and showed that two independent V-ATPase inhibitors (bafilomycin A1 and concanamycin A) are sensitive to SOX11 levels, causing reduced anti-proliferative response in SOX11 low cells. bafilomycin A1 106-120 SRY-box transcription factor 11 Homo sapiens 158-163 27430213-9 2016 Further validation analyses were focused on V-ATPase and showed that two independent V-ATPase inhibitors (bafilomycin A1 and concanamycin A) are sensitive to SOX11 levels, causing reduced anti-proliferative response in SOX11 low cells. bafilomycin A1 106-120 SRY-box transcription factor 11 Homo sapiens 219-224 27113027-5 2016 In addition, Akt reduction in adipose tissues of mice treated with curcumin could be recovered by administration of autophagy inhibitor bafilomycin A1 (BFA). bafilomycin A1 136-150 thymoma viral proto-oncogene 1 Mus musculus 13-16 27226588-6 2016 Although the proteasome-specific inhibitors lactacystin and epoxomicin only moderately increase FoxO1 protein levels, the inhibitors of lysosomal proteolysis bafilomycin A1 and chloroquine restore the decreased FoxO1 levels in Zfat-deficient T cells to levels comparable with those in control cells. bafilomycin A1 158-172 forkhead box O1 Mus musculus 211-216 27113027-5 2016 In addition, Akt reduction in adipose tissues of mice treated with curcumin could be recovered by administration of autophagy inhibitor bafilomycin A1 (BFA). bafilomycin A1 152-155 thymoma viral proto-oncogene 1 Mus musculus 13-16 25981168-4 2015 We found that combined treatment with EGFR TKIs (erlotinib, gefitinib, or lapatinib) and vATPase inhibitors (bafilomycin A1 or concanamycin A) enhanced synergistic cell death compared to treatments with each drug alone. bafilomycin A1 109-123 epidermal growth factor receptor Homo sapiens 38-42 26873723-9 2016 The decline in Cx43-protein levels, both as gap junctions and as hemichannels, could be prevented by the addition of bafilomycin A1, a lysosomal inhibitor, during the complete nutrient starvation period. bafilomycin A1 117-131 gap junction protein alpha 1 Bos taurus 15-19 26757341-11 2016 Small interfering RNA depletion of TPC2 alone or together with TPC1 increased both LC3II and p62 levels under basal conditions and in response to serum starvation, suggesting that, under conditions of severe energy depletion (serum plus glucose starvation), changes in the autophagic flux (as assessed by use of bafilomycin A1) occurred either when TPC1 or TPC2 were downregulated. bafilomycin A1 312-326 two pore segment channel 2 Homo sapiens 35-39 26757341-11 2016 Small interfering RNA depletion of TPC2 alone or together with TPC1 increased both LC3II and p62 levels under basal conditions and in response to serum starvation, suggesting that, under conditions of severe energy depletion (serum plus glucose starvation), changes in the autophagic flux (as assessed by use of bafilomycin A1) occurred either when TPC1 or TPC2 were downregulated. bafilomycin A1 312-326 two pore channel 1 Mus musculus 63-67 25981168-5 2015 Treatment with bafilomycin A1 or concanamycin A led to the induction of Bnip3 expression in an Hif-1alpha dependent manner. bafilomycin A1 15-29 BCL2 interacting protein 3 Homo sapiens 72-77 25981168-5 2015 Treatment with bafilomycin A1 or concanamycin A led to the induction of Bnip3 expression in an Hif-1alpha dependent manner. bafilomycin A1 15-29 hypoxia inducible factor 1 subunit alpha Homo sapiens 95-105 26043797-8 2015 Pretreatment with the autophagy inhibitors LY294002, 3-methyladenine, chloroquine, and bafilomycin A1 enhanced the induction of apoptosis by MHY218, and this was accompanied by an increase in PARP cleavage. bafilomycin A1 87-101 poly(ADP-ribose) polymerase 1 Homo sapiens 192-196 26418500-8 2015 High phosphate further significantly up-regulated the mRNA levels of TGFB1, NFAT5 and SOX9, effects significantly blunted by additional treatment with bafilomycin A1 or methylamine. bafilomycin A1 151-165 transforming growth factor beta 1 Homo sapiens 69-74 25853243-6 2015 We also found that TGFBIp was internalized by caveolae-mediated endocytosis, and the internalized TGFBIp accumulated after treatment with bafilomycin A1, an inhibitor of lysosomal degradation. bafilomycin A1 138-152 transforming growth factor beta induced Homo sapiens 19-25 25853243-6 2015 We also found that TGFBIp was internalized by caveolae-mediated endocytosis, and the internalized TGFBIp accumulated after treatment with bafilomycin A1, an inhibitor of lysosomal degradation. bafilomycin A1 138-152 transforming growth factor beta induced Homo sapiens 98-104 25376834-4 2015 Inhibition of autophagy by bafilomycin A1 or chloroquine treatment during amino acid scarcity abolished mTORC1 signaling, an effect that could be rescued by inhibiting protein synthesis or amino acid supplementation, respectively. bafilomycin A1 27-41 CREB regulated transcription coactivator 1 Mus musculus 104-110 25617421-6 2015 Inhibition of autophagy with a PI3K inhibitor (3-MA), a ROS scavenger (NAC) or autophagosomal-lysosomal fusion inhibitors (bafilomycin A1 and chloroquine) rescued rMIF-induced vascular leakage, suggesting that autophagy mediates MIF-induced vascular leakage. bafilomycin A1 123-137 macrophage migration inhibitory factor Homo sapiens 164-167 25451935-6 2015 In contrast, Ca(2+) transients induced via lysosome-targeted hTPC2 and endolysosome-targeted rTPC3 were abolished by bafilomycin A1 and markedly attenuated by thapsigargin. bafilomycin A1 117-131 two pore segment channel 2 Homo sapiens 61-66 26039928-7 2015 GH3 treatment with bafilomycin A1, a V-ATPase inhibitor, also blocked GH release, indicating mediation via impaired cellular acidification of V-ATPase. bafilomycin A1 19-33 gonadotropin releasing hormone receptor Rattus norvegicus 0-2 25959678-2 2015 Based on studies with the V-ATPase inhibitor BafilomycinA1, lysosomal acidification is also thought to be required for fusion with incoming vesicles from the autophagic and endocytic pathways. bafilomycin A1 45-58 Vacuolar H[+]-ATPase 26kD subunit Drosophila melanogaster 26-34 26418500-8 2015 High phosphate further significantly up-regulated the mRNA levels of TGFB1, NFAT5 and SOX9, effects significantly blunted by additional treatment with bafilomycin A1 or methylamine. bafilomycin A1 151-165 nuclear factor of activated T cells 5 Homo sapiens 76-81 26418500-8 2015 High phosphate further significantly up-regulated the mRNA levels of TGFB1, NFAT5 and SOX9, effects significantly blunted by additional treatment with bafilomycin A1 or methylamine. bafilomycin A1 151-165 SRY-box transcription factor 9 Homo sapiens 86-90 24977403-0 2014 Phagocytosis of bafilomycin A1-treated apoptotic neuroblastoma cells by bone marrow-derived dendritic cells initiates a CD8alpha+ lymphocyte response to neuroblastoma. bafilomycin A1 16-30 CD8 antigen, alpha chain Mus musculus 120-128 25218469-6 2014 In addition, the proteasomal inhibitor lactacystin (5muM) inhibited Ang II-mediated the reduction of mature hERG channel protein, but the lysosomal inhibitor bafilomycin A1 (1muM) had no effect on the protein. bafilomycin A1 158-172 angiotensinogen Homo sapiens 68-74 25181483-7 2014 The inhibition of lysosomal function by monensin or bafilomycin A1 after the occurrence of PrP(Sc) redistribution by CPZ or U18666A partly antagonized PrP(Sc) degradation, suggesting that the transfer of PrP(Sc) to late endosomes/lysosomes, possibly via alteration of the membrane trafficking machinery of cells, leads to PrP(Sc) degradation. bafilomycin A1 52-66 prion protein Mus musculus 91-94 25181483-7 2014 The inhibition of lysosomal function by monensin or bafilomycin A1 after the occurrence of PrP(Sc) redistribution by CPZ or U18666A partly antagonized PrP(Sc) degradation, suggesting that the transfer of PrP(Sc) to late endosomes/lysosomes, possibly via alteration of the membrane trafficking machinery of cells, leads to PrP(Sc) degradation. bafilomycin A1 52-66 prion protein Mus musculus 151-154 25181483-7 2014 The inhibition of lysosomal function by monensin or bafilomycin A1 after the occurrence of PrP(Sc) redistribution by CPZ or U18666A partly antagonized PrP(Sc) degradation, suggesting that the transfer of PrP(Sc) to late endosomes/lysosomes, possibly via alteration of the membrane trafficking machinery of cells, leads to PrP(Sc) degradation. bafilomycin A1 52-66 prion protein Mus musculus 151-154 25181483-7 2014 The inhibition of lysosomal function by monensin or bafilomycin A1 after the occurrence of PrP(Sc) redistribution by CPZ or U18666A partly antagonized PrP(Sc) degradation, suggesting that the transfer of PrP(Sc) to late endosomes/lysosomes, possibly via alteration of the membrane trafficking machinery of cells, leads to PrP(Sc) degradation. bafilomycin A1 52-66 prion protein Mus musculus 151-154 24687431-3 2014 In particular, Concanamycin A (CMA) and Bafilomycin A1 (Baf), inhibitors of vacuolar ATPase (V-ATPase), increased the secretion of CRELD2 without relying on its C-terminal structure. bafilomycin A1 40-54 cysteine rich with EGF like domains 2 Homo sapiens 131-137 24448802-8 2014 The macrolide antibiotic bafilomycin A1 inhibited CS-induced Ca(2+) release and prevented CFTR clearance from the plasma membrane, further linking cytoplasmic Ca(2+) and CFTR internalization. bafilomycin A1 25-39 CF transmembrane conductance regulator Homo sapiens 90-94 24695574-1 2014 ATP6V0C is the bafilomycin A1-binding subunit of vacuolar ATPase, an enzyme complex that critically regulates vesicular acidification. bafilomycin A1 15-29 ATPase H+ transporting V0 subunit c Homo sapiens 0-7 24695574-6 2014 Knockdown of ATP6V0C also sensitized cells to the accumulation of autophagy substrates and a reduction in neurite length following treatment with 1 nM bafilomycin A1, a concentration that did not produce such alterations in non-target control cells. bafilomycin A1 151-165 ATPase H+ transporting V0 subunit c Homo sapiens 13-20 24448802-8 2014 The macrolide antibiotic bafilomycin A1 inhibited CS-induced Ca(2+) release and prevented CFTR clearance from the plasma membrane, further linking cytoplasmic Ca(2+) and CFTR internalization. bafilomycin A1 25-39 CF transmembrane conductance regulator Homo sapiens 170-174 24321340-4 2014 Interestingly, CA-4-mediated apoptotic cell death was further potentiated by pretreatment with autophagy inhibitors (3-methyladenine and bafilomycin A1) or small interfering RNAs against the autophagic genes (Atg5 and Beclin 1). bafilomycin A1 137-151 carbonic anhydrase 4 Homo sapiens 15-19 24547878-9 2014 The autophagy inhibitor bafilomycin A1 (BaF) decreased 1-induced cell viability and increased pp38, pJNK, FasL, caspase-8 activation, and caspase-3 activation. bafilomycin A1 24-38 BAF nuclear assembly factor 1 Homo sapiens 40-43 24547878-9 2014 The autophagy inhibitor bafilomycin A1 (BaF) decreased 1-induced cell viability and increased pp38, pJNK, FasL, caspase-8 activation, and caspase-3 activation. bafilomycin A1 24-38 Fas ligand Homo sapiens 106-110 24547878-9 2014 The autophagy inhibitor bafilomycin A1 (BaF) decreased 1-induced cell viability and increased pp38, pJNK, FasL, caspase-8 activation, and caspase-3 activation. bafilomycin A1 24-38 caspase 8 Homo sapiens 112-121 24547878-9 2014 The autophagy inhibitor bafilomycin A1 (BaF) decreased 1-induced cell viability and increased pp38, pJNK, FasL, caspase-8 activation, and caspase-3 activation. bafilomycin A1 24-38 caspase 3 Homo sapiens 138-147 25484190-6 2014 Here, we attributed SNCA-induced toxicity mainly to secreted species in a cell culture model of SNCA aggregation and in SNCA transgenic mice: We showed that ALP inhibition by bafilomycinA1 reduced intracellular SNCA aggregation but increased secretion of smaller oligomers that exacerbated microenvironmental response including uptake, inflammation, and cellular damage. bafilomycin A1 175-188 synuclein, alpha Mus musculus 20-24 25484190-6 2014 Here, we attributed SNCA-induced toxicity mainly to secreted species in a cell culture model of SNCA aggregation and in SNCA transgenic mice: We showed that ALP inhibition by bafilomycinA1 reduced intracellular SNCA aggregation but increased secretion of smaller oligomers that exacerbated microenvironmental response including uptake, inflammation, and cellular damage. bafilomycin A1 175-188 synuclein, alpha Mus musculus 96-100 25484190-6 2014 Here, we attributed SNCA-induced toxicity mainly to secreted species in a cell culture model of SNCA aggregation and in SNCA transgenic mice: We showed that ALP inhibition by bafilomycinA1 reduced intracellular SNCA aggregation but increased secretion of smaller oligomers that exacerbated microenvironmental response including uptake, inflammation, and cellular damage. bafilomycin A1 175-188 synuclein, alpha Mus musculus 96-100 25484190-6 2014 Here, we attributed SNCA-induced toxicity mainly to secreted species in a cell culture model of SNCA aggregation and in SNCA transgenic mice: We showed that ALP inhibition by bafilomycinA1 reduced intracellular SNCA aggregation but increased secretion of smaller oligomers that exacerbated microenvironmental response including uptake, inflammation, and cellular damage. bafilomycin A1 175-188 synuclein, alpha Mus musculus 96-100 24047499-8 2013 Human subcutaneous fibroblasts respond to bradykinin by releasing ATP via connexin and pannexin hemichannels, since blockade of connexins, with 2-octanol or carbenoxolone, and pannexin-1, with 10Panx, attenuated bradykinin-induced [Ca2+]i plateau, whereas inhibitors of vesicular exocytosis, such as brefeldin A and bafilomycin A1, were inactive. bafilomycin A1 316-330 kininogen 1 Homo sapiens 42-52 23192368-4 2013 K(IR)2.1 trafficking in HEK293 cells was studied by live cell imaging, immunofluorescence microscopy, and Western blot following pharmacological intervention with dynasore (Dyn), chlorpromazine (CPZ), bafilomycin A1 (Baf), or chloroquine (CQ). bafilomycin A1 201-215 potassium inwardly rectifying channel subfamily J member 2 Homo sapiens 0-8 23674090-4 2013 We used bafilomycin A1 (BMA) as a specific inhibitor of Bcl-xL to examine the biological effects in UC cells in vitro and in vivo. bafilomycin A1 8-22 BCL2 like 1 Homo sapiens 56-62 23674090-4 2013 We used bafilomycin A1 (BMA) as a specific inhibitor of Bcl-xL to examine the biological effects in UC cells in vitro and in vivo. bafilomycin A1 24-27 BCL2 like 1 Homo sapiens 56-62 23530046-11 2013 Acidic extracellular pH caused rapid intracellular acidification, and the IL-1beta-inducing effect of acidic medium could be mimicked by acidifying the cytosol with bafilomycin A1, a proton pump inhibitor. bafilomycin A1 165-179 interleukin 1 beta Homo sapiens 74-82 22929013-3 2012 The packaging of glutamate was perturbed by blocking the vesicular glutamate transporter (VGlut) with bafilomycin A1. bafilomycin A1 102-116 Vesicular glutamate transporter Drosophila melanogaster 57-88 23109342-8 2012 Inhibition of RNase L-induced autophagy using Bafilomycin A1 or 3-methyladenine suppressed viral growth in initial stages; in later stages autophagy promoted viral replication dampening the antiviral effect. bafilomycin A1 46-60 ribonuclease L Homo sapiens 14-21 22929013-3 2012 The packaging of glutamate was perturbed by blocking the vesicular glutamate transporter (VGlut) with bafilomycin A1. bafilomycin A1 102-116 Vesicular glutamate transporter Drosophila melanogaster 90-95 22647715-0 2012 Alpha-synuclein aggregation involves a bafilomycin A 1-sensitive autophagy pathway. bafilomycin A1 39-54 synuclein, alpha Mus musculus 0-15 22943412-3 2012 A decrease in iO2 (intracellular O2) to 0-10 muM, induced by Baf, is sufficient for stabilization of HIFs (hypoxia inducible factors) HIF-1alpha and HIF-2alpha, coupled with an increased expression of target genes including GLUT1 (glucose transporter 1), HIF PHD2 (prolyl hydroxylase domain 2) and CAIX (carbonic anhydrase IX). bafilomycin A1 61-64 hypoxia inducible factor 1 subunit alpha Homo sapiens 134-144 22943412-3 2012 A decrease in iO2 (intracellular O2) to 0-10 muM, induced by Baf, is sufficient for stabilization of HIFs (hypoxia inducible factors) HIF-1alpha and HIF-2alpha, coupled with an increased expression of target genes including GLUT1 (glucose transporter 1), HIF PHD2 (prolyl hydroxylase domain 2) and CAIX (carbonic anhydrase IX). bafilomycin A1 61-64 endothelial PAS domain protein 1 Homo sapiens 149-159 22943412-3 2012 A decrease in iO2 (intracellular O2) to 0-10 muM, induced by Baf, is sufficient for stabilization of HIFs (hypoxia inducible factors) HIF-1alpha and HIF-2alpha, coupled with an increased expression of target genes including GLUT1 (glucose transporter 1), HIF PHD2 (prolyl hydroxylase domain 2) and CAIX (carbonic anhydrase IX). bafilomycin A1 61-64 solute carrier family 2 member 1 Homo sapiens 224-229 22943412-3 2012 A decrease in iO2 (intracellular O2) to 0-10 muM, induced by Baf, is sufficient for stabilization of HIFs (hypoxia inducible factors) HIF-1alpha and HIF-2alpha, coupled with an increased expression of target genes including GLUT1 (glucose transporter 1), HIF PHD2 (prolyl hydroxylase domain 2) and CAIX (carbonic anhydrase IX). bafilomycin A1 61-64 solute carrier family 2 member 1 Homo sapiens 231-252 22943412-3 2012 A decrease in iO2 (intracellular O2) to 0-10 muM, induced by Baf, is sufficient for stabilization of HIFs (hypoxia inducible factors) HIF-1alpha and HIF-2alpha, coupled with an increased expression of target genes including GLUT1 (glucose transporter 1), HIF PHD2 (prolyl hydroxylase domain 2) and CAIX (carbonic anhydrase IX). bafilomycin A1 61-64 carbonic anhydrase 9 Homo sapiens 298-302 22943412-3 2012 A decrease in iO2 (intracellular O2) to 0-10 muM, induced by Baf, is sufficient for stabilization of HIFs (hypoxia inducible factors) HIF-1alpha and HIF-2alpha, coupled with an increased expression of target genes including GLUT1 (glucose transporter 1), HIF PHD2 (prolyl hydroxylase domain 2) and CAIX (carbonic anhydrase IX). bafilomycin A1 61-64 carbonic anhydrase 9 Homo sapiens 304-325 22962303-8 2012 Bafilomycin A1, a specific V-ATPase inhibitor, markedly slowed the intracellular pH (pHi) recovery after acid load in human HCC cells and retarded the growth of human HCC in orthotopic xenograft model. bafilomycin A1 0-14 glucose-6-phosphate isomerase Homo sapiens 85-88 21716324-5 2011 Bafilomycin A1, which inhibits endosomal acidification to block the TLR3 pathway, blocked the dsRNA-induced expression of TSLP, IL-8, IFN-beta, and other molecules including the dsRNA sensors, whereas it did not inhibit diacyllipopeptide-induced expression of TSLP and IL-8. bafilomycin A1 0-14 toll like receptor 3 Homo sapiens 68-72 22276961-4 2012 The high-K(+) -evoked overflow of beta-NAD(+) is attenuated by cleavage of SNAP-25 with botulinum neurotoxin A, by inhibition of N-type voltage-dependent Ca(2+) channels with omega-conotoxin GVIA, and by inhibition of the proton gradient of synaptic vesicles with bafilomycin A1, suggesting that beta-NAD(+) is likely released via vesicle exocytosis. bafilomycin A1 266-280 synaptosome associated protein 25 Rattus norvegicus 76-83 23029002-5 2012 Moreover, phosphatidylinositol 3-kinase inhibition with 3-MA or inhibition of endosomal acidification with bafilomycin A1 hindered the release of TF-bearing NETs. bafilomycin A1 107-121 coagulation factor III, tissue factor Homo sapiens 146-148 21716324-5 2011 Bafilomycin A1, which inhibits endosomal acidification to block the TLR3 pathway, blocked the dsRNA-induced expression of TSLP, IL-8, IFN-beta, and other molecules including the dsRNA sensors, whereas it did not inhibit diacyllipopeptide-induced expression of TSLP and IL-8. bafilomycin A1 0-14 thymic stromal lymphopoietin Homo sapiens 122-126 21716324-5 2011 Bafilomycin A1, which inhibits endosomal acidification to block the TLR3 pathway, blocked the dsRNA-induced expression of TSLP, IL-8, IFN-beta, and other molecules including the dsRNA sensors, whereas it did not inhibit diacyllipopeptide-induced expression of TSLP and IL-8. bafilomycin A1 0-14 C-X-C motif chemokine ligand 8 Homo sapiens 128-132 21716324-5 2011 Bafilomycin A1, which inhibits endosomal acidification to block the TLR3 pathway, blocked the dsRNA-induced expression of TSLP, IL-8, IFN-beta, and other molecules including the dsRNA sensors, whereas it did not inhibit diacyllipopeptide-induced expression of TSLP and IL-8. bafilomycin A1 0-14 interferon beta 1 Homo sapiens 134-142 21716324-5 2011 Bafilomycin A1, which inhibits endosomal acidification to block the TLR3 pathway, blocked the dsRNA-induced expression of TSLP, IL-8, IFN-beta, and other molecules including the dsRNA sensors, whereas it did not inhibit diacyllipopeptide-induced expression of TSLP and IL-8. bafilomycin A1 0-14 thymic stromal lymphopoietin Homo sapiens 260-264 21716324-5 2011 Bafilomycin A1, which inhibits endosomal acidification to block the TLR3 pathway, blocked the dsRNA-induced expression of TSLP, IL-8, IFN-beta, and other molecules including the dsRNA sensors, whereas it did not inhibit diacyllipopeptide-induced expression of TSLP and IL-8. bafilomycin A1 0-14 C-X-C motif chemokine ligand 8 Homo sapiens 269-273 21558123-8 2011 Bafilomycin A1 treatment also decreased exocytotic secretion from the regulated pathway, monitored by a CHGA chimera tagged with embryonic alkaline phosphatase. bafilomycin A1 0-14 chromogranin A Homo sapiens 104-108 21807906-6 2011 Bafilomycin A1 (BafA), a specific inhibitor of vacuolar ATPase (vATPase) required for lysosomal function, increased the growth of the human pathogen Chlamydia trachomatis (L2) in wild-type murine fibroblasts and macrophages but inhibited growth in the autophagy-deficient ATG5(-/-) fibroblasts. bafilomycin A1 0-14 ATPase, H+ transporting, lysosomal V0 subunit D2 Mus musculus 47-62 21807906-6 2011 Bafilomycin A1 (BafA), a specific inhibitor of vacuolar ATPase (vATPase) required for lysosomal function, increased the growth of the human pathogen Chlamydia trachomatis (L2) in wild-type murine fibroblasts and macrophages but inhibited growth in the autophagy-deficient ATG5(-/-) fibroblasts. bafilomycin A1 0-14 ATPase, H+ transporting, lysosomal V0 subunit D2 Mus musculus 64-71 21807906-6 2011 Bafilomycin A1 (BafA), a specific inhibitor of vacuolar ATPase (vATPase) required for lysosomal function, increased the growth of the human pathogen Chlamydia trachomatis (L2) in wild-type murine fibroblasts and macrophages but inhibited growth in the autophagy-deficient ATG5(-/-) fibroblasts. bafilomycin A1 16-20 ATPase, H+ transporting, lysosomal V0 subunit D2 Mus musculus 47-62 21807906-6 2011 Bafilomycin A1 (BafA), a specific inhibitor of vacuolar ATPase (vATPase) required for lysosomal function, increased the growth of the human pathogen Chlamydia trachomatis (L2) in wild-type murine fibroblasts and macrophages but inhibited growth in the autophagy-deficient ATG5(-/-) fibroblasts. bafilomycin A1 16-20 ATPase, H+ transporting, lysosomal V0 subunit D2 Mus musculus 64-71 21613417-9 2011 Treatment with bafilomycin A1 (a proton pump inhibitor) and leupeptin (a lysosomal inhibitor) reversed WNK4 WT-mediated inhibition of Maxi K total protein expression. bafilomycin A1 15-29 WNK lysine deficient protein kinase 4 Homo sapiens 103-107 21428909-4 2011 MMP-9 gene induction was sensitive toward treatment with the macrolide antibiotic bafilomycin A1, a vacuolar H(+)-ATPase inhibitor, and with the lysosomotropic agent chloroquine. bafilomycin A1 82-96 matrix metallopeptidase 9 Homo sapiens 0-5 21289198-4 2011 Here, we demonstrate that four independent and highly selective inhibitors of vacuolar ATPase (bafilomycin A1, concanamycin A, archazolid B, and apicularen A) significantly elevate intracellular and secreted GRN. bafilomycin A1 95-109 granulin precursor Homo sapiens 208-211 21303699-4 2011 Moreover, the incubation of cells with ammonium chloride or bafilomycin A1 to produce the lysosomal dysfunction recently reported in Parkinson"s disease led to an increase in the release of alpha-synuclein in exosomes and a concomitant increase in alpha-synuclein transmission to recipient cells. bafilomycin A1 60-74 synuclein alpha Homo sapiens 190-205 21303699-4 2011 Moreover, the incubation of cells with ammonium chloride or bafilomycin A1 to produce the lysosomal dysfunction recently reported in Parkinson"s disease led to an increase in the release of alpha-synuclein in exosomes and a concomitant increase in alpha-synuclein transmission to recipient cells. bafilomycin A1 60-74 synuclein alpha Homo sapiens 248-263 21789015-9 2011 Moreover, treatment with bafilomycin A1 or folimycin restored total NKG2D levels in TGF-BETA1-treated NK cells. bafilomycin A1 25-39 killer cell lectin like receptor K1 Homo sapiens 68-73 21789015-9 2011 Moreover, treatment with bafilomycin A1 or folimycin restored total NKG2D levels in TGF-BETA1-treated NK cells. bafilomycin A1 25-39 transforming growth factor beta 1 Homo sapiens 84-93 19864570-6 2009 The effects of beclin 1 overexpression on LC3 and alpha-syn accumulation were partially blocked by 3-MA and completely blocked by bafilomycin A1. bafilomycin A1 130-144 beclin 1 Homo sapiens 15-23 20570919-8 2010 Furthermore, the inhibition of intracellular acidification by treatment with bafilomycin A1 or chloroquine reproduced the phenotype observed for the (P)RR/ATP6AP2-deficient cardiomyocytes. bafilomycin A1 77-91 ATPase, H+ transporting, lysosomal accessory protein 2 Mus musculus 155-162 20200208-6 2010 However, direct delivery of NAADP, the CD38 metabolite, into CD38(-/-) CAMs still markedly increased Ca(2+) release, which could be significantly attenuated by a lysosomal function inhibitor, bafilomycin A1 (Baf), or a NAADP antagonist, pyridoxalphosphate-6-azophenyl-2-disulfonic acid. bafilomycin A1 192-206 CD38 antigen Mus musculus 39-43 20200208-6 2010 However, direct delivery of NAADP, the CD38 metabolite, into CD38(-/-) CAMs still markedly increased Ca(2+) release, which could be significantly attenuated by a lysosomal function inhibitor, bafilomycin A1 (Baf), or a NAADP antagonist, pyridoxalphosphate-6-azophenyl-2-disulfonic acid. bafilomycin A1 192-206 CD38 antigen Mus musculus 61-65 20200208-6 2010 However, direct delivery of NAADP, the CD38 metabolite, into CD38(-/-) CAMs still markedly increased Ca(2+) release, which could be significantly attenuated by a lysosomal function inhibitor, bafilomycin A1 (Baf), or a NAADP antagonist, pyridoxalphosphate-6-azophenyl-2-disulfonic acid. bafilomycin A1 208-211 CD38 antigen Mus musculus 39-43 20200208-8 2010 In isolated perfused septal coronary arteries from CD38(+/+) mice, FasL was found to significantly increase U-46619-induced vasoconstriction from 29.2 +/- 7.3 to 63.2 +/- 10.3%, which was abolished by Baf (100 nM). bafilomycin A1 201-204 Fas ligand (TNF superfamily, member 6) Mus musculus 67-71 20104024-7 2010 Celecoxib-induced conversion of LC3 was due to autophagy induction, as supported using the lysosome inhibitor, bafilomycin A1, which produced an accumulation of LC3II. bafilomycin A1 111-125 microtubule associated protein 1 light chain 3 alpha Homo sapiens 32-35 19864570-6 2009 The effects of beclin 1 overexpression on LC3 and alpha-syn accumulation were partially blocked by 3-MA and completely blocked by bafilomycin A1. bafilomycin A1 130-144 microtubule-associated protein 1 light chain 3 alpha Mus musculus 42-45 19864570-6 2009 The effects of beclin 1 overexpression on LC3 and alpha-syn accumulation were partially blocked by 3-MA and completely blocked by bafilomycin A1. bafilomycin A1 130-144 synuclein alpha Homo sapiens 50-59 19594752-7 2009 The specific vacuolar ATPase (V-ATPase) inhibitors, bafilomycin A1 or FR167356, prevented vacuolization and drug uptake. bafilomycin A1 52-66 dynein axonemal heavy chain 8 Homo sapiens 22-28 19594752-7 2009 The specific vacuolar ATPase (V-ATPase) inhibitors, bafilomycin A1 or FR167356, prevented vacuolization and drug uptake. bafilomycin A1 52-66 dynein axonemal heavy chain 8 Homo sapiens 30-38 16895977-9 2006 Our findings suggest that the V-ATPase inhibitors bafilomycin A1 and concanamycin A similarly induce NO production and the newly produced NO participates partially in the V-ATPase inhibitor-induced apoptosis in RAW 264.7 cells. bafilomycin A1 50-64 ATPase, H+ transporting, lysosomal V0 subunit D2 Mus musculus 30-38 18632865-3 2008 The entry of ENTV Env pseudovirions was substantially inhibited by bafilomycin A1 (BafA1) but was surprisingly enhanced by lysosomotropic agents and lysosomal protease inhibitors following a 4- to 6-h treatment period; of note, prolonged treatment with BafA1 or ammonium chloride completely blocked ENTV entry. bafilomycin A1 83-88 envelope protein Jaagsiekte sheep retrovirus 18-21 18408955-5 2008 Acidotropic probes (LysoSensor and DAMP) accumulate in non-pigmented Pmel17-positive melanosomes, and DAMP accumulation is sensitive to bafilomycin A1, a specific inhibitor of V-ATPase. bafilomycin A1 136-150 ATPase, H+ transporting, lysosomal V0 subunit D2 Mus musculus 176-184 17638544-4 2007 When the cells were pretreated with two structurally different lysosomal vesicle function inhibitors, bafilomycin A1 (Baf) and glycyl-L-phenylalanine-beta-naphthylamide (GPN), the FasL-induced LRs clustering was substantially blocked, and corresponding ROS production significantly decreased. bafilomycin A1 102-116 Fas ligand Homo sapiens 180-184 19459857-4 2009 We investigated the apoptosis mechanism induced by cotreatment of Bcl-xL-overexpressing Ms-1 cells with BMA as a V-ATPase inhibitor and taxol (TXL) as an anticancer drug. bafilomycin A1 104-107 BCL2 like 1 Homo sapiens 66-72 19448082-8 2009 The primary transport system driving base secretion into the lumen appears to be a bafilomycin-A(1)-sensitive, electrogenic H(+) V-ATPase located on the basal membrane, which extrudes acid into the haemolymph, as inferred from the extracellular pH gradients detected adjacent to the basal membrane. bafilomycin A1 83-99 Vacuolar H[+]-ATPase 55kD subunit Drosophila melanogaster 129-137 17913823-4 2007 The ability of WNV(Vero) to induce IFN-alpha in pDCs did not require viral replication and was prevented by the treatment of cells with bafilomycin A1 and chloroquine, suggesting that it was dependent on endosomal Toll-like receptor recognition. bafilomycin A1 136-150 interferon alpha 1 Homo sapiens 35-44 17604018-4 2007 Treatment with bafilomycin A1, an MMP-2 activator, or GM6001, an MMP inhibitor, at the pre-condensation stage resulted in the inhibition or promotion of chondrogenesis, respectively. bafilomycin A1 15-29 matrix metallopeptidase 2 Homo sapiens 34-39 17431216-7 2007 In contrast, caspase-8 but not caspase-9 activation was blocked by the destruction of lysosomes (preincubation with Gly-Phe beta-naphthylamide, a cathepsin C substrate), loss of lysosomal acidity (bafilomycin A1), or inhibition of cathepsin L or D. Using pepstatin A-BODIPY FL conjugate, we confirmed translocation of cathepsin D out of lysosomes in response to menadione. bafilomycin A1 197-211 caspase 8 Homo sapiens 13-22 16895977-2 2006 Bafilomycin A1 and concanamycin A activated nuclear factor (NF)-kappaB and activator protein-1 and decreased the level of IkappaB-alpha and increased that of phosphorylated c-Jun N-terminal kinase (JNK). bafilomycin A1 0-14 nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha Mus musculus 122-135 16895977-9 2006 Our findings suggest that the V-ATPase inhibitors bafilomycin A1 and concanamycin A similarly induce NO production and the newly produced NO participates partially in the V-ATPase inhibitor-induced apoptosis in RAW 264.7 cells. bafilomycin A1 50-64 ATPase, H+ transporting, lysosomal V0 subunit D2 Mus musculus 171-179 16895977-2 2006 Bafilomycin A1 and concanamycin A activated nuclear factor (NF)-kappaB and activator protein-1 and decreased the level of IkappaB-alpha and increased that of phosphorylated c-Jun N-terminal kinase (JNK). bafilomycin A1 0-14 mitogen-activated protein kinase 8 Mus musculus 173-196 16895977-2 2006 Bafilomycin A1 and concanamycin A activated nuclear factor (NF)-kappaB and activator protein-1 and decreased the level of IkappaB-alpha and increased that of phosphorylated c-Jun N-terminal kinase (JNK). bafilomycin A1 0-14 mitogen-activated protein kinase 8 Mus musculus 198-201 16443004-8 2006 K-independent proton transport activity was significantly inhibited by the H(+)-ATPase inhibitor bafilomycin A(1) (BAF, 10 nM) with luminal applied in both WT and KO mice. bafilomycin A1 97-113 b-associated fitness Mus musculus 115-118 16473958-8 2006 In the presence of Baf (100 nM), this ET-1-induced two-phase [Ca2+]i response was completely abolished, whereas Rya (50 microM) only markedly blocked the ET-1-induced large global Ca2+ increase. bafilomycin A1 19-22 endothelin 1 Homo sapiens 38-42 16473958-8 2006 In the presence of Baf (100 nM), this ET-1-induced two-phase [Ca2+]i response was completely abolished, whereas Rya (50 microM) only markedly blocked the ET-1-induced large global Ca2+ increase. bafilomycin A1 19-22 endothelin 1 Homo sapiens 154-158 16473958-9 2006 Functional studies showed that 100 nM Baf significantly attenuated ET-1-induced maximal constriction from 82.26 +/- 4.42% to 51.80 +/- 4.36%. bafilomycin A1 38-41 endothelin 1 Homo sapiens 67-71 16622236-7 2006 The IL-8 secretion in response to P. aeruginosa DNA was decreased by treatments that inhibit acidification of intracellular organelles, using chloroquine, a pH-neutralizing compound, or bafilomycin A1, an inhibitor of vacuolar H+-ATPase. bafilomycin A1 186-200 C-X-C motif chemokine ligand 8 Homo sapiens 4-8 16227281-10 2005 By using small interfering RNA against the mu2 subunit of adaptor protein 2, dominant negative dynamin construct K44A, and the lysosomotropic agents bafilomycin A1 and ammonium chloride, we also demonstrated that surface TNFR1 was internalized by RID by a clathrin-dependent process involving mu2 and dynamin, followed by degradation of TNFR1 via an endosomal/lysosomal pathway. bafilomycin A1 149-163 TNF receptor superfamily member 1A Homo sapiens 221-226 16133868-5 2005 Although we found no evidence for a direct involvement of lysosomal cathepsins, bafilomycin A1 and ammonium chloride significantly attenuated TNF toxicity. bafilomycin A1 80-94 tumor necrosis factor Rattus norvegicus 142-145 15847604-10 2005 Thrombin, but not ADP or AVP, reduces the rise in [Ca2+]c evoked by nigericin and bafilomycin A1. bafilomycin A1 82-96 coagulation factor II, thrombin Homo sapiens 0-8 15847604-10 2005 Thrombin, but not ADP or AVP, reduces the rise in [Ca2+]c evoked by nigericin and bafilomycin A1. bafilomycin A1 82-96 carbonic anhydrase 2 Homo sapiens 51-54 15542860-7 2005 The effect of bafilomycin A1 on CgA secretion depends on a sorting determinant located within the amino terminus (CgA-(1-115)) but not the C-terminal region of the granin. bafilomycin A1 14-28 chromogranin A Rattus norvegicus 32-35 15885844-9 2005 The PLL-SU5%/DNA complex seems to be internalized via SUR-mediated endocytosis pathway as suggested by vacuolar ATPases inhibition by Bafilomycin A1. bafilomycin A1 134-148 ATP binding cassette subfamily C member 8 Rattus norvegicus 54-57 15542860-7 2005 The effect of bafilomycin A1 on CgA secretion depends on a sorting determinant located within the amino terminus (CgA-(1-115)) but not the C-terminal region of the granin. bafilomycin A1 14-28 chromogranin A Rattus norvegicus 114-117 15638326-0 2004 Biosynthetic investigations of the V-type ATPase inhibitors bafilomycin A1, B1 and concanamycin A. bafilomycin A1 60-74 dynein axonemal heavy chain 8 Homo sapiens 42-48 12675272-10 2003 Similarly, pHi recovery from NH4Cl prepulse acid-loads (pHo 7.4) was sensitive to both EIPA and bafilomycin A1. bafilomycin A1 96-110 glucose-6-phosphate isomerase Homo sapiens 11-14 15102616-6 2004 Furthermore, whereas the internalized receptors exhibit rapid recycling, treatment with proton pump inhibitors (bafilomycin-A1 and concanamycin A) or brefeldin A, Golgi disrupting agents, reduces PTH/PTHrP receptor recycling. bafilomycin A1 112-126 parathyroid hormone 1 receptor Homo sapiens 196-214 14561721-10 2003 These findings suggest that bafilomycin A(1)-induced apoptosis in MKN-1 cells is mediated by other proteases released after lysosomal dysfunction followed by activation of caspase-3 in a cytochrome c-independent manner. bafilomycin A1 28-44 caspase 3 Homo sapiens 172-181 14561721-10 2003 These findings suggest that bafilomycin A(1)-induced apoptosis in MKN-1 cells is mediated by other proteases released after lysosomal dysfunction followed by activation of caspase-3 in a cytochrome c-independent manner. bafilomycin A1 28-44 cytochrome c, somatic Homo sapiens 187-199 15166000-4 2004 Correspondingly, the amount of small responsive GLUT4 vesicles in concanamycin A- and bafilomycin A1-treated cells is decreased. bafilomycin A1 86-100 solute carrier family 2 member 4 Homo sapiens 48-53 14985124-3 2004 The UGA4 protein was located on the vacuolar membrane as determined by the effects of bafilomycin A1 and by indirect immunofluorescence microscopy. bafilomycin A1 86-100 Uga4p Saccharomyces cerevisiae S288C 4-8 12900525-8 2003 The recognition of HSV-2 by TLR9 was mediated through an endocytic pathway that was inhibited by chloroquine or bafilomycin A1. bafilomycin A1 112-126 toll like receptor 9 Homo sapiens 28-32 12547648-6 2003 Likewise, another V-type ATPase inhibitor, bafilomycinA1 facilitated GDNF release from C6, U87MG and T98G glioma cells, in a concentration-dependent manner. bafilomycin A1 43-56 glial cell derived neurotrophic factor Homo sapiens 69-73 12675272-4 2003 Bafilomycin A1 and 5-(N-ethyl-N-isopropyl)amiloride (EIPA) were used to delineate the activities of the H+-pump and NHE, respectively. bafilomycin A1 0-14 solute carrier family 9 member C1 Homo sapiens 116-119 12498152-4 2002 Based on these results and comparing them to existing models of the adenovirus entry, we propose a refined model in which there are two pathways of adenoviral entry: the first one involves the epsilon-COP as the downstream effector of the acidification and can be blocked by bafilomycin A1 and the second one is a pH-independent pathway. bafilomycin A1 275-289 coatomer subunit epsilon Cricetulus griseus 193-204 11773703-3 2002 Exposure of neonatal rat cardiomyocytes to the established apoptotic agents, bafilomycin A1 (BAF) or staurosporine (STAU) induced apoptosis and caused a decrease in PP-1 activity of 35%. bafilomycin A1 77-91 BAF nuclear assembly factor 1 Rattus norvegicus 93-96 11773703-3 2002 Exposure of neonatal rat cardiomyocytes to the established apoptotic agents, bafilomycin A1 (BAF) or staurosporine (STAU) induced apoptosis and caused a decrease in PP-1 activity of 35%. bafilomycin A1 77-91 neuropeptide Y receptor Y4 Rattus norvegicus 165-169 11799150-7 2002 Inhibition of adenovirus penetration with bafilomycin A1 or ammonium chloride attenuated the activation of ERK-p38 and IP-10 mRNA expression following infection, suggesting that endosomal escape was required to trigger these pathways. bafilomycin A1 42-56 mitogen-activated protein kinase 1 Homo sapiens 107-110 11799150-7 2002 Inhibition of adenovirus penetration with bafilomycin A1 or ammonium chloride attenuated the activation of ERK-p38 and IP-10 mRNA expression following infection, suggesting that endosomal escape was required to trigger these pathways. bafilomycin A1 42-56 mitogen-activated protein kinase 1 Homo sapiens 111-114 11799150-7 2002 Inhibition of adenovirus penetration with bafilomycin A1 or ammonium chloride attenuated the activation of ERK-p38 and IP-10 mRNA expression following infection, suggesting that endosomal escape was required to trigger these pathways. bafilomycin A1 42-56 C-X-C motif chemokine ligand 10 Homo sapiens 119-124 11812790-5 2002 The translocation of tyrosinase out of the endoplasmic reticulum and the induction of cell pigmentation in the presence of the ionophore monensin or the specific V-ATPase inhibitors concanamycin A and bafilomycin A1 supported a role for V-ATPases in this process. bafilomycin A1 201-215 tyrosinase Homo sapiens 21-31 11812790-5 2002 The translocation of tyrosinase out of the endoplasmic reticulum and the induction of cell pigmentation in the presence of the ionophore monensin or the specific V-ATPase inhibitors concanamycin A and bafilomycin A1 supported a role for V-ATPases in this process. bafilomycin A1 201-215 dynein axonemal heavy chain 8 Homo sapiens 162-170 11432741-7 2001 Inhibitor studies revealed that the heterologously produced A1 ATPase is insensitive to azide, dicyclohexylcarbodiimide and bafilomycin A1, but sensitive to diethylstilbestrol and its analogues dienestrol and hexestrol. bafilomycin A1 124-138 ATPase Escherichia coli 63-69 10922469-1 2000 In this study, we describe the activation of melanogenesis by selective vacuolar type H(+)-ATPase inhibitors (bafilomycin A1 and concanamycin A) in amelanotic human and mouse melanoma cells which express tyrosinase but show no melanogenesis. bafilomycin A1 110-124 tyrosinase Mus musculus 204-214 11390405-5 2001 Bafilomycin A1, but not a specific proteasome inhibitor, prevented early degradation of palmitoylation-deficient CCR5 and promoted its accumulation in lysosomal compartments. bafilomycin A1 0-14 C-C motif chemokine receptor 5 Homo sapiens 113-117 10231559-0 1999 Induction of hydroxyapatite resorptive activity in bone marrow cell populations resistant to bafilomycin A1 by a factor with restricted expression to bone and brain, neurochondrin. bafilomycin A1 93-107 neurochondrin Mus musculus 166-179 10784591-6 2000 Bafilomycin A1 was used to analyze the association between GFP-ATP7B and the late endosomes. bafilomycin A1 0-14 ATPase copper transporting beta Homo sapiens 59-68 10446931-10 1999 These calcitonin effects on endocytotic and re-exocytotic pathways were inhibited by 100 nM cytochalasin D, 100 nM nocodazole, 0.1 to 1 microM bafilomycin A1, or 0.1 mM monodansyl cadaverine. bafilomycin A1 143-157 Calcitonin gene-related peptide Sus scrofa 6-16 10231559-4 1999 By means of this method, we isolated a factor with 16% leucine residues, termed neurochondrin, that induces hydroxyapatite resorptive activity in bone marrow cells resistant to bafilomycin A1, an inhibitor of macrophage- and osteoclast-mediated resorption. bafilomycin A1 177-191 neurochondrin Mus musculus 80-93 10195692-5 1999 When cells were treated with the fungal metabolite brefeldin A or with the specific vacuolar H+-ATPase inhibitor bafilomycin A1, the processing of SgII and the release of its cleavage products were strongly inhibited, indicating that its processing commenced in the later compartments of the secretory pathway. bafilomycin A1 113-127 secretogranin II S homeolog Xenopus laevis 147-151 10217271-4 1999 The macrolides bafilomycin A1 (BAF) and concanamycin A (CON) block lysosomal acidification through selective inhibition of the V-type ATPase. bafilomycin A1 15-29 BAF nuclear assembly factor 1 Rattus norvegicus 31-34 10201953-5 1999 Using whole blood from healthy donors and flow cytometry, we found that colchicine (0.1-10 mM), cytochalasin D (1000 microM), NH4Cl (10-50 mM), and bafilomycin A1 (0.1-3 microM) significantly reduced the affinity of FITC-HBP for CD14-positive monocytes. bafilomycin A1 148-162 azurocidin 1 Homo sapiens 221-224 10201953-5 1999 Using whole blood from healthy donors and flow cytometry, we found that colchicine (0.1-10 mM), cytochalasin D (1000 microM), NH4Cl (10-50 mM), and bafilomycin A1 (0.1-3 microM) significantly reduced the affinity of FITC-HBP for CD14-positive monocytes. bafilomycin A1 148-162 CD14 molecule Homo sapiens 229-233 10201953-6 1999 Using isolated human monocytes and ELISA, we found that colchicine (0.1 mM), cytochalasin D (30 and 300 microM), NH4Cl (30 mM), and bafilomycin A1 (1 microM) significantly reduced the effect of HBP (10 microg/ml) to enhance LPS (10 ng/ml)-induced TNF-alpha release after 24 h. These findings demonstrate that internalization of HBP in monocytes is essential for the enhancement of LPS-induced TNF-alpha release. bafilomycin A1 132-146 azurocidin 1 Homo sapiens 194-197 10201953-6 1999 Using isolated human monocytes and ELISA, we found that colchicine (0.1 mM), cytochalasin D (30 and 300 microM), NH4Cl (30 mM), and bafilomycin A1 (1 microM) significantly reduced the effect of HBP (10 microg/ml) to enhance LPS (10 ng/ml)-induced TNF-alpha release after 24 h. These findings demonstrate that internalization of HBP in monocytes is essential for the enhancement of LPS-induced TNF-alpha release. bafilomycin A1 132-146 tumor necrosis factor Homo sapiens 247-256 10201953-6 1999 Using isolated human monocytes and ELISA, we found that colchicine (0.1 mM), cytochalasin D (30 and 300 microM), NH4Cl (30 mM), and bafilomycin A1 (1 microM) significantly reduced the effect of HBP (10 microg/ml) to enhance LPS (10 ng/ml)-induced TNF-alpha release after 24 h. These findings demonstrate that internalization of HBP in monocytes is essential for the enhancement of LPS-induced TNF-alpha release. bafilomycin A1 132-146 azurocidin 1 Homo sapiens 328-331 10201953-6 1999 Using isolated human monocytes and ELISA, we found that colchicine (0.1 mM), cytochalasin D (30 and 300 microM), NH4Cl (30 mM), and bafilomycin A1 (1 microM) significantly reduced the effect of HBP (10 microg/ml) to enhance LPS (10 ng/ml)-induced TNF-alpha release after 24 h. These findings demonstrate that internalization of HBP in monocytes is essential for the enhancement of LPS-induced TNF-alpha release. bafilomycin A1 132-146 tumor necrosis factor Homo sapiens 393-402 9657902-6 1998 Following acid loading in NaCl Ringers with a 20 mm NH4Cl prepulse, pHi recovery was partially inhibited by exposure to either Na-free (NMGCl) Ringers, 100 microM DMA or 20 microM bafilomycin A1. bafilomycin A1 180-194 glucose-6-phosphate isomerase Bos taurus 68-71 9808725-5 1998 45Ca2+ pumping into vesicles from ECA1 transformants was detected after the H+/Ca2+-antiport activity was eliminated with bafilomycin A1 and gramicidin D. The pump had a high affinity for Ca2+ (Km = 30 nM) and displayed two affinities for ATP (Km of 20 and 235 microM). bafilomycin A1 122-136 ER-type Ca2+-ATPase 1 Arabidopsis thaliana 34-38 9321906-6 1997 In the absence of peritubular sodium, pHi recovery was inhibited by luminal bafilomycin A1 but not by luminal Sch-28080 addition or by luminal potassium removal. bafilomycin A1 76-90 glucose-6-phosphate isomerase Oryctolagus cuniculus 38-41 9374657-8 1997 Bafilomycin A1 (0.1 microM; an inhibitor of vacuolar H(+)-ATPase) inhibited the pHi increase caused by high K+. bafilomycin A1 0-14 glucose-6-phosphate isomerase Oryctolagus cuniculus 80-83 9502788-5 1998 Accordingly, bafilomycin A1-treated myocytes also showed increased accumulation of p53 protein and p53-dependent transactivation of gene expression, including a persistent upregulation of p21/wild-type p53 activated fragment 1/cyclin kinase inhibitor protein-1 mRNA. bafilomycin A1 13-27 tumor protein p53 Homo sapiens 83-86 9502788-5 1998 Accordingly, bafilomycin A1-treated myocytes also showed increased accumulation of p53 protein and p53-dependent transactivation of gene expression, including a persistent upregulation of p21/wild-type p53 activated fragment 1/cyclin kinase inhibitor protein-1 mRNA. bafilomycin A1 13-27 tumor protein p53 Homo sapiens 99-102 9502788-5 1998 Accordingly, bafilomycin A1-treated myocytes also showed increased accumulation of p53 protein and p53-dependent transactivation of gene expression, including a persistent upregulation of p21/wild-type p53 activated fragment 1/cyclin kinase inhibitor protein-1 mRNA. bafilomycin A1 13-27 cyclin dependent kinase inhibitor 1A Homo sapiens 188-191 9502788-5 1998 Accordingly, bafilomycin A1-treated myocytes also showed increased accumulation of p53 protein and p53-dependent transactivation of gene expression, including a persistent upregulation of p21/wild-type p53 activated fragment 1/cyclin kinase inhibitor protein-1 mRNA. bafilomycin A1 13-27 tumor protein p53 Homo sapiens 99-102 9207248-3 1997 Addition of 10(-8) M bafilomycin A1 to osteoclasts cultured on bone induced a rapid decrease of the pHi, while addition of amiloride had only a minor effect. bafilomycin A1 21-35 glucose-6-phosphate isomerase Rattus norvegicus 100-103 9200012-2 1997 Specific inhibitors of vacuolar H(+)-ATPase (V-ATPase), concanamycin A and bafilomycin A1, abolish bone resorption by osteoclasts. bafilomycin A1 75-89 ATPase, H+ transporting, lysosomal V0 subunit D2 Mus musculus 23-43 9153255-5 1997 We show that removal of transferrin from sorting endosomes and accumulation in the peri-centriolar endocytic recycling compartment takes place normally in bafilomycin A1-treated cells. bafilomycin A1 155-169 transferrin Homo sapiens 24-35 8943309-6 1996 Treatment of fibroblasts with either bafilomycin A1 or 3-methyladenine indicates that proteolytic cleavage of SAP precursor begins as early as in the late endosomes. bafilomycin A1 37-51 SH2 domain containing 1A Homo sapiens 110-113 9151711-5 1997 Inhibitors of lysosomal function (bafilomycin A1, folimycin) markedly reduced zeta chain degradation, leading to the accumulation of zeta chain in large Lamp1(+) vesicles. bafilomycin A1 34-48 lysosomal associated membrane protein 1 Homo sapiens 153-158 9157016-7 1997 Using antibodies against the myc epitope and the early endosomal autoantigen EEA1 as markers, we found that endosomes in wild-type and mutant MDCK cells rapidly tubulate in the presence of bafilomycin A1, an inhibitor of vacuolar H(+)-ATPase. bafilomycin A1 189-203 early endosome antigen 1 Canis lupus familiaris 77-81 8941214-6 1996 BafilomycinA1 and NH4Cl, both of which raise the intragranular pH to neutral, inhibited the eosinophil peroxidase exocytosis induced by platelet-activating factor. bafilomycin A1 0-13 eosinophil peroxidase Homo sapiens 92-113 8910293-4 1996 The transport of [3H]ACh by human VAChT was dependent upon the addition of exogenous ATP at 37 degrees C. Uptake was abolished by low temperature (4 degrees C), the proton ionophore carbonyl cyanide p-trifluoromethoxyphenylhydrazone (2.5 microM) and bafilomycin A1 (1 microM), a specific inhibitor of the vesicular H+-ATPase. bafilomycin A1 250-264 solute carrier family 18 member A3 Homo sapiens 34-39 8626546-7 1996 Treatment of the cells with bafilomycin A1, an inhibitor for the proton pump of the lysosomes, inhibited intracellular degradation of the LDL and prevented down-regulations of the mRNA and the cell surface TM antigen levels caused by oxidized LDL. bafilomycin A1 28-42 thrombomodulin Homo sapiens 206-208 8741216-5 1996 Thus, quantitative lmmunogold labeling showed that the Baf-induced alkalinization reduced the transfer of internalized Cat.Au to lysosomes, as defined by their content of human lysosome-associated membrane protein 1 (h-lamp-1) and cathepsin D. bafilomycin A1 55-58 lysosomal associated membrane protein 1 Homo sapiens 177-215 8741216-5 1996 Thus, quantitative lmmunogold labeling showed that the Baf-induced alkalinization reduced the transfer of internalized Cat.Au to lysosomes, as defined by their content of human lysosome-associated membrane protein 1 (h-lamp-1) and cathepsin D. bafilomycin A1 55-58 cathepsin D Homo sapiens 231-242 7852298-4 1995 In contrast, the robust production of A beta from a human neuroglioma-derived cell line (HS683) transfected with WT APP, or from primary human mixed brain cultures (HMBC) expressing genomic WT APP, were also effectively inhibited by baf A. bafilomycin A1 233-238 amyloid beta precursor protein Homo sapiens 38-44 8546711-5 1996 Here we have shown that bafilomycin A1, a specific inhibitor of V-ATPase, inhibits endosome acidification and mitogen-induced DNA synthesis in Swiss 3T3 fibroblasts. bafilomycin A1 24-38 ATPase, H+ transporting, lysosomal V0 subunit D2 Mus musculus 64-72 7642700-3 1995 Toward this purpose, bafilomycin A1 (Baf), a specific inhibitor of the vacuolar proton pump, was used to inhibit acidification of the vacuolar system. bafilomycin A1 21-35 BAF nuclear assembly factor 1 Homo sapiens 37-40 7890753-0 1995 The vacuolar H(+)-ATPase inhibitor bafilomycin A1 differentially affects proteolytic processing of mutant and wild-type beta-amyloid precursor protein. bafilomycin A1 35-49 amyloid beta precursor protein Homo sapiens 120-150 7890753-1 1995 We analyzed the effect of the vacuolar H(+)-ATPase inhibitor bafilomycin A1 (bafA1) on the processing of beta-amyloid precursor protein (beta APP). bafilomycin A1 61-75 amyloid beta precursor protein Homo sapiens 105-135 7890753-1 1995 We analyzed the effect of the vacuolar H(+)-ATPase inhibitor bafilomycin A1 (bafA1) on the processing of beta-amyloid precursor protein (beta APP). bafilomycin A1 77-82 amyloid beta precursor protein Homo sapiens 105-135 7864795-12 1995 We conclude that the TRHR-TRH system is unique among recycling receptors because, after RL sequestration, the TRHR-TRH complex becomes dissociated intracellularly via a bafilomycin A1-sensitive, acidic pH-dependent mechanism, and both the unoccupied TRHR and TRH recycle disassociated from each other. bafilomycin A1 169-183 thyrotropin releasing hormone receptor Rattus norvegicus 21-25 7864795-12 1995 We conclude that the TRHR-TRH system is unique among recycling receptors because, after RL sequestration, the TRHR-TRH complex becomes dissociated intracellularly via a bafilomycin A1-sensitive, acidic pH-dependent mechanism, and both the unoccupied TRHR and TRH recycle disassociated from each other. bafilomycin A1 169-183 thyrotropin releasing hormone receptor Rattus norvegicus 110-114 7864795-12 1995 We conclude that the TRHR-TRH system is unique among recycling receptors because, after RL sequestration, the TRHR-TRH complex becomes dissociated intracellularly via a bafilomycin A1-sensitive, acidic pH-dependent mechanism, and both the unoccupied TRHR and TRH recycle disassociated from each other. bafilomycin A1 169-183 thyrotropin releasing hormone receptor Rattus norvegicus 110-114 7840150-13 1995 Bafilomycin A1 and pCMBS did not inhibit the initial amiloride-sensitive portion of recovery, but they did inhibit a late component of recovery when pHi was above 7.0. bafilomycin A1 0-14 glucose-6-phosphate isomerase 1 Mus musculus 149-152 7864640-3 1995 Under these conditions IL-1-stimulated proteoglycan degradation was inhibited by bafilomycin A1 with an IC50 of < 10 nM in both chondrocyte monolayers and articular cartilage explants. bafilomycin A1 81-95 interleukin 1 alpha Homo sapiens 23-27 7864640-6 1995 Tumor necrosis factor-alpha and retinoic acid also stimulated proteoglycan degradation in chondrocyte monolayers, and in both cases the response was inhibited by bafilomycin A1. bafilomycin A1 162-176 tumor necrosis factor Homo sapiens 0-27 7864640-8 1995 IL-1 also stimulated the synthesis and secretion of prostromelysin by chondrocyte monolayers, however, under conditions in which IL-1 stimulated proteoglycan release was totally blocked by bafilomycin A1, there was no effect on IL-1-stimulated stromelysin secretion or stromelysin enzyme activity. bafilomycin A1 189-203 interleukin 1 alpha Homo sapiens 0-4 7864640-8 1995 IL-1 also stimulated the synthesis and secretion of prostromelysin by chondrocyte monolayers, however, under conditions in which IL-1 stimulated proteoglycan release was totally blocked by bafilomycin A1, there was no effect on IL-1-stimulated stromelysin secretion or stromelysin enzyme activity. bafilomycin A1 189-203 interleukin 1 alpha Homo sapiens 129-133 7864640-8 1995 IL-1 also stimulated the synthesis and secretion of prostromelysin by chondrocyte monolayers, however, under conditions in which IL-1 stimulated proteoglycan release was totally blocked by bafilomycin A1, there was no effect on IL-1-stimulated stromelysin secretion or stromelysin enzyme activity. bafilomycin A1 189-203 interleukin 1 alpha Homo sapiens 129-133 1660409-1 1991 Bafilomycin A1, a selective inhibitor of vacuolar H(+)-ATPase, induced neurite outgrowth of PC12 cells dose- and time-dependently: more than 50% of the cells extended neurite-like spikes after 24 h treatment with 100 nM bafilomycin A1. bafilomycin A1 220-234 UDP glucuronosyltransferase family 1 member A6 Rattus norvegicus 12-61 7518670-1 1994 We examined the effect of bafilomycin A1 (BAF), an inhibitor of vacuolar-type H(+)-ATPases, on macrophages activation (measured as increased nitrite production and leishmanicidal activity) induced by interferon gamma alone or together with lipopolysaccharide or tumour necrosis factor alpha. bafilomycin A1 26-40 BAF nuclear assembly factor 1 Homo sapiens 42-45 8169833-1 1994 Bafilomycin A1, an inhibitor of vacuolar adenosine triphosphatase, was tested for its ability to antagonize botulinum neurotoxins (serotypes A-G), tetanus toxin and phospholipase A2 neurotoxins (notexin, beta-bungarotoxin, taipoxin and textilotoxin) on the mouse phrenic nerve-hemidiaphragm preparation. bafilomycin A1 0-14 phospholipase A2, group IB, pancreas Mus musculus 165-181 8253856-7 1993 The functional importance of V-type H+ ATPase-activity in preserving pHi homeostasis at acidic extracellular pH levels was reflected by the impairment of O2- production at pHo 6.70 when H+ ATPase activity was inhibited: bafilomycin A1 reduced O2- production from 13.9 +/- 1.0 to 9.3 +/- 0.6 nmoles/10(6) cells/40 min, in control and bafilomycin A1-treated cells, respectively (P < 0.05), while EPA had no effect. bafilomycin A1 220-234 glucose-6-phosphate isomerase 1 Mus musculus 69-72 1314493-3 1992 Second, at 37 degrees C, pHi recovery after acute intracellular acidification caused by 40 mM acetate addition to cell suspension was inhibited 36% by 200-400 nM bafilomycin A1, a macrolide antibiotic that specifically inhibits vacuolar-type H(+)-ATPase at submicromolar concentrations. bafilomycin A1 162-176 glucose-6-phosphate isomerase Rattus norvegicus 25-28 8006950-5 1994 (iii) The internal application of the vacuolar H(+)-ATPase inhibitors N-ethylmaleimide (NEM), NO3- and bafilomycin A1 (BFA) depolarized the membrane by 15 +/- 2, 18 +/- 2 and 16 +/- 2 mV, respectively. bafilomycin A1 119-122 NBL1, DAN family BMP antagonist Mus musculus 94-97 1317456-3 1992 In the absence of Na+ in the superfusion solutions, pHi recovered from the acid load by a mechanism inhibited by 0.1 microM bafilomycin A1, a specific inhibitor of a vacuolar-type H(+)-ATPase. bafilomycin A1 124-138 glucose-6-phosphate isomerase Rattus norvegicus 52-55 33236955-3 2021 NH4Cl and bafilomycin A1, but not the proteasomal inhibitor MG132, prevented the FAC-mediated decrease in TfR1 protein levels, thus indicating lysosomal involvement. bafilomycin A1 10-24 transferrin receptor Homo sapiens 106-110 1832676-11 1991 In the presence of 1 microM bafilomycin A1, 125I-labeled epidermal growth factor (EGF) bound to the cell surface at 4 degrees C was internalized normally into the cells at 37 degrees C but was not degraded at all, in marked contrast to the rapid degradation of 125I-EGF in the control cells without the drug. bafilomycin A1 28-42 epidermal growth factor Mus musculus 57-80 1832676-11 1991 In the presence of 1 microM bafilomycin A1, 125I-labeled epidermal growth factor (EGF) bound to the cell surface at 4 degrees C was internalized normally into the cells at 37 degrees C but was not degraded at all, in marked contrast to the rapid degradation of 125I-EGF in the control cells without the drug. bafilomycin A1 28-42 epidermal growth factor Mus musculus 82-85 1832676-11 1991 In the presence of 1 microM bafilomycin A1, 125I-labeled epidermal growth factor (EGF) bound to the cell surface at 4 degrees C was internalized normally into the cells at 37 degrees C but was not degraded at all, in marked contrast to the rapid degradation of 125I-EGF in the control cells without the drug. bafilomycin A1 28-42 epidermal growth factor Mus musculus 266-269 1651328-5 1991 Furthermore, although no specific protease inhibitor assayed could affect this processing, NGFR degradation and truncation were retarded by treatment with: 1) the weak base amines, ammonium chloride or methylamine; 2) the carboxylic ionophore, monensin; or 3) the vacuolar ATPase inhibitor, bafilomycin A1, all agents that dissipate endosomal/lysosomal proton gradients via alternate mechanisms. bafilomycin A1 291-305 nerve growth factor receptor Homo sapiens 91-95 34933095-7 2022 In Abeta(1-42)-, A53T-alpha-synuclein-, or Q74-induced BV-2 cells, PSS significantly inhibited NLRP3 inflammasome activation, which was attenuated by bafilomycin A1 (an autophagy inhibitor) and SHP099 (an SHP-2 inhibitor). bafilomycin A1 150-164 NLR family, pyrin domain containing 3 Mus musculus 95-100 34905649-6 2022 Moreover, rapamycin successfully restores the impaired autophagic flux and alleviates liver senescence in GDF11 overexpression mice, while the GDF11 knockdown-mediated benefits are abolished by the autophagy inhibitor bafilomycin A1. bafilomycin A1 218-232 growth differentiation factor 11 Mus musculus 143-148 34425162-4 2021 Pretreatment of HNE cells with the specific vacuolar H+-ATPase inhibitor bafilomycin A1 reduced the RV-C03 RNA levels in the ASL; inflammatory cytokines, including interleukin (IL)-1beta, IL-6 and IL-8, in the supernatant; the mRNA expression of the RV-C receptor cadherin-related family member 3 (CDHR3) in the cells; and the number of acidic endosomes where RV-B RNA enters the cytoplasm. bafilomycin A1 73-87 interleukin 1 alpha Homo sapiens 164-186 34105096-13 2022 The intervention of bafilomycin A1 and rapamycin in HSCs strongly supported the notion that inhibition of autophagy enhanced alpha -SMA protein expression levels (P<0.01). bafilomycin A1 20-34 immunoglobulin mu binding protein 2 Mus musculus 132-135 34520793-5 2021 Our results showed that DM enhanced autophagy in association with an accumulation of autophagosomes and increase in the levels of autophagy markers LC3-II/LC3-I ratio and p62 which were much elevated in the presence of lysosomal inhibitors bafilomycin A1 and chloroquine. bafilomycin A1 240-254 nucleoporin 62 Homo sapiens 171-174 34954677-4 2021 BPA treatment significantly increased the levels of autophagy-regulated proteins including Beclin-1 and LC3-II along with the decrease of p62, accompanied by the elevation of autophagy flux and the accumulation of acidic vacuoles, which was blocked by the autophagy inhibitor bafilomycin A1 (BafA1). bafilomycin A1 276-290 beclin 1 Homo sapiens 91-99 34954677-4 2021 BPA treatment significantly increased the levels of autophagy-regulated proteins including Beclin-1 and LC3-II along with the decrease of p62, accompanied by the elevation of autophagy flux and the accumulation of acidic vacuoles, which was blocked by the autophagy inhibitor bafilomycin A1 (BafA1). bafilomycin A1 276-290 microtubule associated protein 1 light chain 3 alpha Homo sapiens 104-107 34954677-4 2021 BPA treatment significantly increased the levels of autophagy-regulated proteins including Beclin-1 and LC3-II along with the decrease of p62, accompanied by the elevation of autophagy flux and the accumulation of acidic vacuoles, which was blocked by the autophagy inhibitor bafilomycin A1 (BafA1). bafilomycin A1 276-290 nucleoporin 62 Homo sapiens 138-141 34954677-4 2021 BPA treatment significantly increased the levels of autophagy-regulated proteins including Beclin-1 and LC3-II along with the decrease of p62, accompanied by the elevation of autophagy flux and the accumulation of acidic vacuoles, which was blocked by the autophagy inhibitor bafilomycin A1 (BafA1). bafilomycin A1 292-297 beclin 1 Homo sapiens 91-99 34954677-4 2021 BPA treatment significantly increased the levels of autophagy-regulated proteins including Beclin-1 and LC3-II along with the decrease of p62, accompanied by the elevation of autophagy flux and the accumulation of acidic vacuoles, which was blocked by the autophagy inhibitor bafilomycin A1 (BafA1). bafilomycin A1 292-297 microtubule associated protein 1 light chain 3 alpha Homo sapiens 104-107 34954677-4 2021 BPA treatment significantly increased the levels of autophagy-regulated proteins including Beclin-1 and LC3-II along with the decrease of p62, accompanied by the elevation of autophagy flux and the accumulation of acidic vacuoles, which was blocked by the autophagy inhibitor bafilomycin A1 (BafA1). bafilomycin A1 292-297 nucleoporin 62 Homo sapiens 138-141 34536391-11 2021 Bafilomycin A1 (BafA1) is a late autophagy inhibitor, aggravated cell damage and apoptosis and counteracted the enhancement of autophagy activation and protective effects of neuritin. bafilomycin A1 0-14 neuritin 1 Homo sapiens 174-182 34536391-11 2021 Bafilomycin A1 (BafA1) is a late autophagy inhibitor, aggravated cell damage and apoptosis and counteracted the enhancement of autophagy activation and protective effects of neuritin. bafilomycin A1 16-21 neuritin 1 Homo sapiens 174-182 34425162-4 2021 Pretreatment of HNE cells with the specific vacuolar H+-ATPase inhibitor bafilomycin A1 reduced the RV-C03 RNA levels in the ASL; inflammatory cytokines, including interleukin (IL)-1beta, IL-6 and IL-8, in the supernatant; the mRNA expression of the RV-C receptor cadherin-related family member 3 (CDHR3) in the cells; and the number of acidic endosomes where RV-B RNA enters the cytoplasm. bafilomycin A1 73-87 interleukin 6 Homo sapiens 188-192 34425162-4 2021 Pretreatment of HNE cells with the specific vacuolar H+-ATPase inhibitor bafilomycin A1 reduced the RV-C03 RNA levels in the ASL; inflammatory cytokines, including interleukin (IL)-1beta, IL-6 and IL-8, in the supernatant; the mRNA expression of the RV-C receptor cadherin-related family member 3 (CDHR3) in the cells; and the number of acidic endosomes where RV-B RNA enters the cytoplasm. bafilomycin A1 73-87 C-X-C motif chemokine ligand 8 Homo sapiens 197-201 34425162-4 2021 Pretreatment of HNE cells with the specific vacuolar H+-ATPase inhibitor bafilomycin A1 reduced the RV-C03 RNA levels in the ASL; inflammatory cytokines, including interleukin (IL)-1beta, IL-6 and IL-8, in the supernatant; the mRNA expression of the RV-C receptor cadherin-related family member 3 (CDHR3) in the cells; and the number of acidic endosomes where RV-B RNA enters the cytoplasm. bafilomycin A1 73-87 cadherin related family member 3 Homo sapiens 298-303 34127226-4 2021 Further studies show that fucoidan promotes autophagy showed by the expression of p62/SQSTM1 and microtubule-associated protein light chain 3 (LC3) II, which can be blocked by autophagy inhibitors 3-MA and bafilomycin A1. bafilomycin A1 206-220 sequestosome 1 Homo sapiens 82-85 34434265-8 2021 The decrease in apoptosis caused by IKKepsilon knockdown was reversed when the autophagic flow was blocked using bafilomycin A1. bafilomycin A1 113-127 inhibitor of nuclear factor kappa B kinase subunit epsilon Homo sapiens 36-46 34127226-4 2021 Further studies show that fucoidan promotes autophagy showed by the expression of p62/SQSTM1 and microtubule-associated protein light chain 3 (LC3) II, which can be blocked by autophagy inhibitors 3-MA and bafilomycin A1. bafilomycin A1 206-220 sequestosome 1 Homo sapiens 86-92 34127226-4 2021 Further studies show that fucoidan promotes autophagy showed by the expression of p62/SQSTM1 and microtubule-associated protein light chain 3 (LC3) II, which can be blocked by autophagy inhibitors 3-MA and bafilomycin A1. bafilomycin A1 206-220 microtubule associated protein 1 light chain 3 alpha Homo sapiens 143-146 34571747-10 2021 Immunofluorescence evidenced the accumulation of LC3B puncta in U87Mg cells pretreated with autophagy inhibitor Bafilomycin A1. bafilomycin A1 112-126 microtubule associated protein 1 light chain 3 beta Homo sapiens 49-53 34062228-6 2021 In conclusion, claudin-1 might be a barrier for CSFV infection in PK-15 cells, while CSFV bypasses the barrier through lysosome mediated degradation of claudin-1, which could be repressed by bafilomycin A1. bafilomycin A1 191-205 claudin 1 Sus scrofa 15-24 34410949-11 2021 Autophagy inhibitors including 3-methyladenine (3-MA), chloroquine (CQ) and bafilomycin A1 (Baf) significantly alleviated the autophagic cell death effect of gadodiamide on normal brain glial SVG P12 cells. bafilomycin A1 76-90 BAF nuclear assembly factor 1 Homo sapiens 92-95 34378314-8 2021 The results suggested that silencing of SLC26A4-AS1 led to declined ITPR1 level, up-regulation of ETS1 promoted ITPR1 expression, and either ETS1 knockdown or autophagy inhibitor Bafilomycin A1 could mitigate the promoting effects of SLC26A4-AS1 overexpression on PTC cell autophagy. bafilomycin A1 179-193 SLC26A4 antisense RNA 1 Homo sapiens 234-245 34062228-6 2021 In conclusion, claudin-1 might be a barrier for CSFV infection in PK-15 cells, while CSFV bypasses the barrier through lysosome mediated degradation of claudin-1, which could be repressed by bafilomycin A1. bafilomycin A1 191-205 claudin 1 Sus scrofa 152-161 34360859-6 2021 This process was suppressed by knockdown of the Cx43 gene and by bafilomycin A1, an inhibitor of vacuolar ATPase. bafilomycin A1 65-79 gap junction protein alpha 1 Homo sapiens 48-52 34252168-5 2021 Endosomal acidification inhibitors like BafilomycinA1 and NH4Cl, which inhibit the CG pathway, reduce the uptake of RBD and impede Spike-pseudoviral infection in both AGS and AGS-ACE2 cells. bafilomycin A1 40-53 surface glycoprotein Severe acute respiratory syndrome coronavirus 2 131-136 34335562-8 2021 Additionally, analysis of nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain-containing 3 (NLRP3) and NLR with CARD domain-containing 4 (NLRC4) in J774A.1 cells indicated that spvC decreased the protein levels of NLRP3 and NLRC4, which were significantly changed by autophagy inhibitor Bafilomycin A1. bafilomycin A1 315-329 NLR family, pyrin domain containing 3 Mus musculus 120-125 34335562-8 2021 Additionally, analysis of nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain-containing 3 (NLRP3) and NLR with CARD domain-containing 4 (NLRC4) in J774A.1 cells indicated that spvC decreased the protein levels of NLRP3 and NLRC4, which were significantly changed by autophagy inhibitor Bafilomycin A1. bafilomycin A1 315-329 NLR family, CARD domain containing 4 Mus musculus 166-171 34335562-8 2021 Additionally, analysis of nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain-containing 3 (NLRP3) and NLR with CARD domain-containing 4 (NLRC4) in J774A.1 cells indicated that spvC decreased the protein levels of NLRP3 and NLRC4, which were significantly changed by autophagy inhibitor Bafilomycin A1. bafilomycin A1 315-329 Salmonella plasmid virulence protein C Salmonella enterica subsp. enterica serovar Typhimurium 205-209 34335562-8 2021 Additionally, analysis of nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain-containing 3 (NLRP3) and NLR with CARD domain-containing 4 (NLRC4) in J774A.1 cells indicated that spvC decreased the protein levels of NLRP3 and NLRC4, which were significantly changed by autophagy inhibitor Bafilomycin A1. bafilomycin A1 315-329 NLR family, pyrin domain containing 3 Mus musculus 242-247 34335562-8 2021 Additionally, analysis of nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain-containing 3 (NLRP3) and NLR with CARD domain-containing 4 (NLRC4) in J774A.1 cells indicated that spvC decreased the protein levels of NLRP3 and NLRC4, which were significantly changed by autophagy inhibitor Bafilomycin A1. bafilomycin A1 315-329 NLR family, CARD domain containing 4 Mus musculus 252-257 34252168-5 2021 Endosomal acidification inhibitors like BafilomycinA1 and NH4Cl, which inhibit the CG pathway, reduce the uptake of RBD and impede Spike-pseudoviral infection in both AGS and AGS-ACE2 cells. bafilomycin A1 40-53 angiotensin converting enzyme 2 Homo sapiens 179-183 34185060-9 2021 The decreased levels of ATP7A and Atox1 proteins were restored by the antioxidant N-acetylcysteine and the lysosomal inhibitor bafilomycin A1. bafilomycin A1 127-141 ATPase copper transporting alpha Homo sapiens 24-29 34252168-6 2021 The inhibition by BafilomycinA1 was found to be distinct from Chloroquine which neither affects RBD uptake nor alters endosomal pH, yet attenuates Spike-pseudovirus entry. bafilomycin A1 18-31 surface glycoprotein Severe acute respiratory syndrome coronavirus 2 147-152 34185060-9 2021 The decreased levels of ATP7A and Atox1 proteins were restored by the antioxidant N-acetylcysteine and the lysosomal inhibitor bafilomycin A1. bafilomycin A1 127-141 antioxidant 1 copper chaperone Homo sapiens 34-39 34093174-9 2021 In addition, when the fusion of autophagosomes with lysosomes was blocked by Bafilomycin A1, the degradation of p62 induced by Dex during the autophagy process was suspended. bafilomycin A1 77-91 nucleoporin 62 Homo sapiens 112-115 34080667-7 2021 In addition, pretreatment with the autophagy inhibitor bafilomycin A1 attenuated CXCR4 abrogation-induced cell death. bafilomycin A1 55-69 chemokine (C-X-C motif) receptor 4 Mus musculus 81-86 34177609-6 2021 Interestingly, nicotine significantly promoted dynamin-related protein 1 (Drp1)-mediated mitochondrial fission and suppressed mitofusin (MFN)-mediated fusion, which was also observed in the bafilomycin A1-treated group. bafilomycin A1 190-204 dynamin 1-like Rattus norvegicus 47-72 34257560-12 2021 After treatment with bafilomycin A1, proliferation was inhibited in both cell types, but the effects were more prominent in the cells overexpressing CUL4A. bafilomycin A1 21-35 cullin 4A Homo sapiens 149-154 34079802-6 2021 Inhibition of autophagy by inhibitors like leupeptin, chloroquine (CQ), and Bafilomycin A1 (Baf-A1) blocks the degradation of B7H3 in glioma cells. bafilomycin A1 76-90 CD276 antigen Mus musculus 126-130 34079802-6 2021 Inhibition of autophagy by inhibitors like leupeptin, chloroquine (CQ), and Bafilomycin A1 (Baf-A1) blocks the degradation of B7H3 in glioma cells. bafilomycin A1 92-98 CD276 antigen Mus musculus 126-130 34062977-6 2021 Using our existing protocol, inflammasome activation was induced in IL-1alpha-primed ARPE-19 cells with the proteasome and autophagy inhibitors MG-132 and bafilomycin A1, respectively. bafilomycin A1 155-169 interleukin 1 alpha Homo sapiens 68-77 34184748-8 2021 Mechanistically, TMEM100 was demonstrated to induce autophagy in A549 cells via inhibiting the PI3K/AKT signaling pathway, whereas inhibiting autophagy using bafilomycin A1 significantly enhanced TMEM100-induced apoptosis to compensate for the cell death. bafilomycin A1 158-172 transmembrane protein 100 Homo sapiens 196-203 35092164-7 2022 While blocking the fusion of autophagosomes with lysosomes by bafilomycin A1(BafA1), the reduced NLRP3 inflammasome activity induced by RAPA was significantly restored, with increased protein levels of NLRP3 and cleaved Casp-1(p10), as well as IL-1beta secretion. bafilomycin A1 62-76 NLR family pyrin domain containing 3 Homo sapiens 97-102 35366539-10 2022 Moreover, treatment with bafilomycin A1, an inhibitor of lysosomal protein degradation, restored Glut1 protein levels by siRNA of LDHA. bafilomycin A1 25-39 solute carrier family 2 (facilitated glucose transporter), member 1 Mus musculus 97-102 35366539-10 2022 Moreover, treatment with bafilomycin A1, an inhibitor of lysosomal protein degradation, restored Glut1 protein levels by siRNA of LDHA. bafilomycin A1 25-39 lactate dehydrogenase A Mus musculus 130-134 35435531-7 2022 Deletion of mTOR relieved autophagosome-lysosome fusion dysfunction and ameliorated apoptosis of CD4 + T cells in CLP mice, but this rescued phenotype was abolished by treatment with bafilomycin A1, a specific A-L fusion inhibitor. bafilomycin A1 183-197 mechanistic target of rapamycin kinase Mus musculus 12-16 35435531-7 2022 Deletion of mTOR relieved autophagosome-lysosome fusion dysfunction and ameliorated apoptosis of CD4 + T cells in CLP mice, but this rescued phenotype was abolished by treatment with bafilomycin A1, a specific A-L fusion inhibitor. bafilomycin A1 183-197 CD4 antigen Mus musculus 97-100 35122618-6 2022 LC3 II was increased at 8 h (p = 0.043) and 24 h (p = 0.015) of recovery, whereas p62 was elevated at 2 h (p = 0.002) and 8 h (p < 0.001) of recovery, but returned to baseline by 24 h. In Bafilomycin A1 treated cells, p62 was further increased immediately following HT (p = 0.041). bafilomycin A1 188-202 microtubule associated protein 1 light chain 3 alpha Homo sapiens 0-3 35122618-6 2022 LC3 II was increased at 8 h (p = 0.043) and 24 h (p = 0.015) of recovery, whereas p62 was elevated at 2 h (p = 0.002) and 8 h (p < 0.001) of recovery, but returned to baseline by 24 h. In Bafilomycin A1 treated cells, p62 was further increased immediately following HT (p = 0.041). bafilomycin A1 188-202 nucleoporin 62 Homo sapiens 82-85 35092164-7 2022 While blocking the fusion of autophagosomes with lysosomes by bafilomycin A1(BafA1), the reduced NLRP3 inflammasome activity induced by RAPA was significantly restored, with increased protein levels of NLRP3 and cleaved Casp-1(p10), as well as IL-1beta secretion. bafilomycin A1 62-76 NLR family pyrin domain containing 3 Homo sapiens 202-207 35092164-7 2022 While blocking the fusion of autophagosomes with lysosomes by bafilomycin A1(BafA1), the reduced NLRP3 inflammasome activity induced by RAPA was significantly restored, with increased protein levels of NLRP3 and cleaved Casp-1(p10), as well as IL-1beta secretion. bafilomycin A1 62-76 caspase 1 Homo sapiens 220-226 35092164-7 2022 While blocking the fusion of autophagosomes with lysosomes by bafilomycin A1(BafA1), the reduced NLRP3 inflammasome activity induced by RAPA was significantly restored, with increased protein levels of NLRP3 and cleaved Casp-1(p10), as well as IL-1beta secretion. bafilomycin A1 62-76 S100 calcium binding protein A10 Homo sapiens 227-230 35092164-7 2022 While blocking the fusion of autophagosomes with lysosomes by bafilomycin A1(BafA1), the reduced NLRP3 inflammasome activity induced by RAPA was significantly restored, with increased protein levels of NLRP3 and cleaved Casp-1(p10), as well as IL-1beta secretion. bafilomycin A1 62-76 interleukin 1 alpha Homo sapiens 244-252 35179568-4 2022 Inhibition of autophagic flux by chloroquine or bafilomycin A1 were sufficient to induce eNOS monomerization and lowers nitric oxide bioavailability through raising mitochondrial reactive oxygen species (mtROS). bafilomycin A1 48-62 nitric oxide synthase 3 Homo sapiens 89-93 35624155-9 2022 Pharmacological inhibition of autophagy by bafilomycin A1 increased polyUb-p62 and polyUb-p53 in the cytoplasm, indicating that p53 protein levels and subcellular localization were regulated by polyUb-p62 and autophagy. bafilomycin A1 43-57 sequestosome 1 Mus musculus 75-78 35624155-9 2022 Pharmacological inhibition of autophagy by bafilomycin A1 increased polyUb-p62 and polyUb-p53 in the cytoplasm, indicating that p53 protein levels and subcellular localization were regulated by polyUb-p62 and autophagy. bafilomycin A1 43-57 transformation related protein 53, pseudogene Mus musculus 90-93 35624155-9 2022 Pharmacological inhibition of autophagy by bafilomycin A1 increased polyUb-p62 and polyUb-p53 in the cytoplasm, indicating that p53 protein levels and subcellular localization were regulated by polyUb-p62 and autophagy. bafilomycin A1 43-57 transformation related protein 53, pseudogene Mus musculus 128-131 35007347-5 2022 In contrast, the inhibitors of endosomal acidification bafilomycin A1 and chloroquine increased levels of LC3-II, due to reduced degradation in acidic lysosomes. bafilomycin A1 55-69 microtubule associated protein 1 light chain 3 alpha Homo sapiens 106-112 35348729-8 2022 The increased efficacy of mTOR inhibition plus T-DM1 was abrogated by lysosome inhibitors (chloroquine and bafilomycin A1). bafilomycin A1 107-121 mechanistic target of rapamycin kinase Homo sapiens 26-30 35387562-9 2022 Thus, we demonstrate that bafilomycin A1 treatment from 2.5 up to 24 h does not inhibit fusion between autophagosomes and lysosomes, but leads to the accumulation of LC3-positive material inside autolysosomes. bafilomycin A1 26-40 microtubule associated protein 1 light chain 3 alpha Homo sapiens 166-169 35023829-5 2022 Phenotypical analysis of pikfyve zebrafish mutants revealed that loss of Pikfyve caused aberrant vacuolation (accumulation of Rab7+Lc3+ amphisomes) in lens cells, which was significantly alleviated by treatment with the V-ATPase inhibitor bafilomycin A1 (Baf-A1). bafilomycin A1 239-253 phosphoinositide kinase, FYVE finger containing Danio rerio 25-32 35023829-5 2022 Phenotypical analysis of pikfyve zebrafish mutants revealed that loss of Pikfyve caused aberrant vacuolation (accumulation of Rab7+Lc3+ amphisomes) in lens cells, which was significantly alleviated by treatment with the V-ATPase inhibitor bafilomycin A1 (Baf-A1). bafilomycin A1 239-253 phosphoinositide kinase, FYVE finger containing Danio rerio 73-80 34996966-5 2022 When rapamycin was combined with saturating concentrations of BafA, no significant further increase of LC3B lipidation could be detected over the levels induced by the late-stage inhibitor. bafilomycin A1 62-66 microtubule associated protein 1 light chain 3 beta Homo sapiens 103-107 34996966-3 2022 Here we scrutinize this approach and show that bafilomycin A1 (BafA) but not chloroquine is suitable for flux quantification due to the stimulating effect of chloroquine on non-canonical LC3B-lipidation. bafilomycin A1 47-61 microtubule associated protein 1 light chain 3 beta Homo sapiens 187-191 34996966-3 2022 Here we scrutinize this approach and show that bafilomycin A1 (BafA) but not chloroquine is suitable for flux quantification due to the stimulating effect of chloroquine on non-canonical LC3B-lipidation. bafilomycin A1 63-67 microtubule associated protein 1 light chain 3 beta Homo sapiens 187-191