PMID-sentid	Pub_year	Sent_text	compound_name	comp_offset	prot_official_name	organism	prot_offset
19452580-7	2009	Mean mucin concentration measured in bile by the Sepharose CL-4B method was 22.8 +/- 24.0 mg/mL (range 3.4-89.0 mg/mL).	Sepharose CL 4B	49-64	LOC100508689	Homo sapiens	5-10
22982755-7	2012	The ScFv-Fc protein was purified to more than 95% purity using a two-step scheme: ammonium sulfate fractionation and Protein A Sepharose CL-4B.	Sepharose CL 4B	127-142	immunglobulin heavy chain variable region	Homo sapiens	4-8
18602477-4	2008	The purification of Hu BChE was accomplished by batch adsorption on procainamide-Sepharose-CL-4B affinity gel followed by ion-exchange chromatography on a DEAE-Sepharose column.	Sepharose CL 4B	81-96	butyrylcholinesterase	Homo sapiens	23-27
12376018-1	2002	Different ligand densities of monoclonal antibody (Mab) CB.Hep-1 were studied during covalent coupling on Sepharose CL-4B for recombinant hepatitis B surface antigen (rHBsAg) immunoaffinity purification.	Sepharose CL 4B	106-121	DNL-type zinc finger	Homo sapiens	59-64
17385041-6	2007	Mucin content was evaluated by gel filtration on a Sepharose CL-4B column.	Sepharose CL 4B	51-66	LOC100508689	Homo sapiens	0-5
16598740-5	2006	The selected Mab were immobilized in a Sepharose CL-4B activated matrix with the purpose of purify PSA from human seminal fluid.	Sepharose CL 4B	39-54	kallikrein related peptidase 3	Homo sapiens	99-102
15178109-4	2004	Sharpnose shark, Rhizoprionodon terraenovae, serum C-reactive protein was purified sequentially over AH-sepharose 4B-PC and sepharose CL-4B columns and used to immunize balb/c mice for generating stocks of polyclonal anti-sera.	Sepharose CL 4B	124-139	C-reactive protein, pentraxin-related	Mus musculus	51-69
17669278-0	2007	Rat liver 10-formyltetrahydrofolate dehydrogenase, carbamoyl phosphate synthetase 1 and betaine homocysteine S-methytransferase were co-purified on Kunitz-type soybean trypsin inhibitor-coupled sepharose CL-4B.	Sepharose CL 4B	194-209	aldehyde dehydrogenase 1 family, member L1	Rattus norvegicus	10-49
17361093-3	2007	In this study human milk mucin, free of contaminant protein and of sufficient quantity for further analysis, was isolated and purified by Sepharose CL-4B gel filtration and cesiumchloride density-gradient centrifugation.	Sepharose CL 4B	138-153	LOC100508689	Homo sapiens	25-30
17020812-1	2006	CvL, a lectin from the marine sponge Cliona varians was purified by acetone fractionation followed by Sepharose CL 4B affinity chromatography.	Sepharose CL 4B	102-117	ezrin	Homo sapiens	0-3
16464473-6	2006	The most enriched NTE active fraction was further purified by 3-9"-mercaptononylthio-1,1,1-trifluoropropan-2-one bound to sepharose CL4B.	Sepharose CL 4B	122-136	patatin-like phospholipase domain containing 6	Rattus norvegicus	18-21
16108496-3	2004	The crude NDPK-A was purified by ion exchange chromatography with DEAE Sepharose Fast Flow, affinity chromatography with Cibarcron Blue 3GA Sepharose CL-4B and gel filtration with Sephadex G-100.	Sepharose CL 4B	140-155	NME/NM23 nucleoside diphosphate kinase 1	Homo sapiens	10-16
12954382-1	2003	In the present study antileukemic enzyme L-asparaginase (ASNase) and catalase (as a model enzyme) were modified in solid-phase with activated polyethylene glycol (PEG(2)) by using ligand-immobilized affinity column systems L-asparagine-Sepharose CL-4B and Procion red-Sepharose CL-4B, respectively.	Sepharose CL 4B	236-251	catalase	Mus musculus	69-77
12954382-1	2003	In the present study antileukemic enzyme L-asparaginase (ASNase) and catalase (as a model enzyme) were modified in solid-phase with activated polyethylene glycol (PEG(2)) by using ligand-immobilized affinity column systems L-asparagine-Sepharose CL-4B and Procion red-Sepharose CL-4B, respectively.	Sepharose CL 4B	236-251	insulin-like growth factor 2	Mus musculus	163-169
12954382-1	2003	In the present study antileukemic enzyme L-asparaginase (ASNase) and catalase (as a model enzyme) were modified in solid-phase with activated polyethylene glycol (PEG(2)) by using ligand-immobilized affinity column systems L-asparagine-Sepharose CL-4B and Procion red-Sepharose CL-4B, respectively.	Sepharose CL 4B	268-283	catalase	Mus musculus	69-77
12954382-1	2003	In the present study antileukemic enzyme L-asparaginase (ASNase) and catalase (as a model enzyme) were modified in solid-phase with activated polyethylene glycol (PEG(2)) by using ligand-immobilized affinity column systems L-asparagine-Sepharose CL-4B and Procion red-Sepharose CL-4B, respectively.	Sepharose CL 4B	268-283	insulin-like growth factor 2	Mus musculus	163-169
12835897-4	2003	The amount of (3)H-mucin was measured by Sepharose CL-4B gel-filtration column chromatography.	Sepharose CL 4B	41-56	LOC100508689	Homo sapiens	19-24
11396878-3	2001	Maleic anhydride (MA) modification of Sepharose CL-4B, cellulose, and Toyopearl HW-65F resulted in reduced generation of C3a, a marker of complement activation, by two orders of magnitude over unmodified surfaces.	Sepharose CL 4B	38-53	complement C3	Homo sapiens	121-124
11931374-2	2002	For extracorporeal adsorption of fibrinogen the pentapeptide gly-pro-arg-pro-lys was coupled to sepharose CL-4B.	Sepharose CL 4B	96-111	fibrinogen beta chain	Homo sapiens	33-43
11778917-3	2001	Adsorbers containing 135 ml of coupled sepharose CL-4B were used to eliminate fibrinogen from the plasma of 7 men and 3 women (48-75 years old).	Sepharose CL 4B	39-54	fibrinogen beta chain	Homo sapiens	78-88
11425799-5	2001	Mucin was purified using Sepharose CL-4B gel filtration.	Sepharose CL 4B	25-40	LOC100508689	Homo sapiens	0-5
10384403-11	1999	Proteins migrating in SDS-polyacrylamide gels in positions similar to the alpha 5 and beta 1 integrin subunits were present in fractions bound to a column of CAP coupled to Sepharose CL-4B.	Sepharose CL 4B	173-188	3-hydroxyacyl-CoA dehydratase 1	Homo sapiens	158-161
12903404-2	2000	METHODS: The experiment was carried out under the low water activity condition, using tosylate chloride activating side-chain hydroxyl group of Sepharose CL-4B agarose to form a high active group which could react with the free amino-group of ACE to link the enzyme with agarose.	Sepharose CL 4B	144-159	angiotensin I converting enzyme	Homo sapiens	243-246
9178925-1	1997	In order to establish the measurement of gastric mucin secreted from cultured mucous cells, rat gastric mucin was purified from secreted mucus with Sepharose CL-4B column chromatography.	Sepharose CL 4B	148-163	solute carrier family 13 member 2	Rattus norvegicus	104-109
9538271-6	1998	About 60% of the hydrolase activity in mouse osteoclast-like cells was immunoabsorbed by anti-cathepsin K antibody-coupled Sepharose CL-4B beads, and about 10% of the activity was absorbed with the anti-cathepsin L antibody-coupled beads.	Sepharose CL 4B	123-138	cathepsin K	Mus musculus	94-105
9581563-6	1997	Biglycan and decorin were immunoisolated from the second Sepharose CL-4B peak, and had average glycosaminoglycan hydrodynamic sizes of approx.	Sepharose CL 4B	57-72	biglycan	Mus musculus	0-8
9260911-6	1997	The immunoprecipitated HSPG eluted from a Sepharose CL-4B with a Kav of 0.44.	Sepharose CL 4B	42-57	syndecan 2	Mus musculus	23-27
7591682-5	1995	Mucin synthesis was estimated by measuring the amount of [3H]glucosamine-labeled glycoprotein excluded on a Sepharose CL-4B column.	Sepharose CL 4B	108-123	LOC100508689	Homo sapiens	0-5
9109822-4	1997	When native MG1 was placed in 4 M guanidine hydrochloride and chromatographed on Sepharose CL-4B, ELISA measurement of column fractions showed that amylase, PRPs, statherins, and histatins were released.	Sepharose CL 4B	81-96	mucin 5B, oligomeric mucus/gel-forming	Homo sapiens	12-15
8255167-2	1994	The receptors were isolated from human full-term placenta by solubilization of trophoblast plasma membranes with the nonionic detergent C12E8 and then by affinity chromatography on a diferric transferrin-coupled Sepharose CL-4B column.	Sepharose CL 4B	212-227	transferrin	Homo sapiens	192-203
8012654-3	1994	A large heparan sulfate proteoglycan (HSPG, 0.2 Kav on Sepharose CL-4B) that was extractable from mouse uterine epithelium with 4 M guanidine-HCl or 1 M KCl was recognized by a specific monoclonal antibody to the basal lamina HSPG, perlecan.	Sepharose CL 4B	55-70	syndecan 2	Mus musculus	8-36
8012654-3	1994	A large heparan sulfate proteoglycan (HSPG, 0.2 Kav on Sepharose CL-4B) that was extractable from mouse uterine epithelium with 4 M guanidine-HCl or 1 M KCl was recognized by a specific monoclonal antibody to the basal lamina HSPG, perlecan.	Sepharose CL 4B	55-70	syndecan 2	Mus musculus	38-42
8572596-5	1995	Concurrently, mucin synthesis was assessed by size exclusion chromatography of [3H]-glucosamine-labeled glycoprotein in a Sepharose CL-4B column.	Sepharose CL 4B	122-137	LOC100508689	Homo sapiens	14-19
1511431-2	1992	Mucin was quantitated by [3H]glucosamine labeling and chromatography on Sepharose CL-4B.	Sepharose CL 4B	72-87	LOC100508689	Homo sapiens	0-5
8482190-2	1993	Mucin was isolated by gel filtration on Sepharose CL-4B and a subsequent CsCl density gradient ultracentrifugation.	Sepharose CL 4B	40-55	LOC100508689	Homo sapiens	0-5
1487514-3	1992	To study and minimize this leakage, a matrix, Sepharose CL-4B, was activated by various chemical reagents and coupled to goat anti-apolipoprotein B polyclonal antibodies.	Sepharose CL 4B	46-61	apolipoprotein B	Homo sapiens	131-147
8443822-3	1993	Mucin was quantitated by [3H]glucosamine labeling and Sepharose CL-4B chromatography.	Sepharose CL 4B	54-69	LOC100508689	Homo sapiens	0-5
1893621-1	1991	We have developed a quantitative assay for IgG autoantibodies against IL-1 alpha using protein A-Sepharose CL-4B.	Sepharose CL 4B	97-112	interleukin 1 alpha	Homo sapiens	70-80
1583313-4	1992	This assay was used to evaluate SpA leakage when purifying a serum-free murine IgG1 cell culture supernatant using SpA immobilized on agarose (Protein A-Sepharose CL-4B) or controlled pore glass (Prosep A, high capacity).	Sepharose CL 4B	153-168	surfactant associated protein A1	Mus musculus	32-35
1421223-2	1992	Method I: two-stage ultracentrifugation with subsequent gel-filtration of Lp(a) containing fractions (1.063-1.090 g/ml) on Sepharose CL-4B.	Sepharose CL 4B	123-138	lipoprotein(a)	Homo sapiens	74-79
1372136-3	1992	Canine CRP was isolated by ion-exchange chromatography using DEAE-Sephacel and DEAE-Sephadex A-50 and affinity chromatography using protein A-Sepharose CL 4B in combination with agar-block electrophoresis.	Sepharose CL 4B	142-157	C-reactive protein	Canis lupus familiaris	7-10
1728605-2	1992	Mucin, quantitated by 3H-glucosamine labelling and chromatography on Sepharose CL-4B was found to be produced by all 4 cell lines.	Sepharose CL 4B	69-84	LOC100508689	Homo sapiens	0-5
1543865-1	1992	To investigate the relationship between biliary mucin and ductular stone formation, mucin was isolated from hepatic bile using gel filtration on Sepharose CL-4B.	Sepharose CL 4B	145-160	LOC100508689	Homo sapiens	84-89
2483162-2	1989	This is applied to the diffusion of porcine serum proteins, gamma-globulin and fibrinogen in Sepharose CL-4B.	Sepharose CL 4B	93-108	fibrinogen beta chain	Homo sapiens	79-89
2061562-1	1991	A monoclonal antibody, coupled to Sepharose CL-4B, was used for the rapid purification of the human multicatalytic proteinase in a single chromatographic step under mild conditions.	Sepharose CL 4B	34-49	endogenous retrovirus group K member 25	Homo sapiens	115-125
1704234-1	1990	A highly specific monoclonal antibody (anti-AFP) against alpha-fetoprotein (AFP) was linked to N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP) to form conjugates which were purified with a protein A-sepharose CL-4B affinity column.	Sepharose CL 4B	203-218	alpha fetoprotein	Homo sapiens	44-47
1704234-1	1990	A highly specific monoclonal antibody (anti-AFP) against alpha-fetoprotein (AFP) was linked to N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP) to form conjugates which were purified with a protein A-sepharose CL-4B affinity column.	Sepharose CL 4B	203-218	alpha fetoprotein	Homo sapiens	57-74
1704234-1	1990	A highly specific monoclonal antibody (anti-AFP) against alpha-fetoprotein (AFP) was linked to N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP) to form conjugates which were purified with a protein A-sepharose CL-4B affinity column.	Sepharose CL 4B	203-218	alpha fetoprotein	Homo sapiens	76-79
1704234-3	1990	The immunotoxin, anti-AFP-abrin-A conjugate, which was also purified with a protein A-sepharose CL-4B affinity column, had a molecular weight of 180,000 and had 80% antigen-binding activity that of anti-AFP activity and 92% toxicity of abrin-A chain.	Sepharose CL 4B	86-101	alpha fetoprotein	Homo sapiens	22-25
2391420-2	1990	Two subpopulations of IgG, M1-I and M1-II, were obtained from serum of MRL-lpr/lpr mice by column chromatography on protein A-Sepharose CL-4B.	Sepharose CL 4B	126-141	immunoglobulin heavy chain (V7183 family)	Mus musculus	22-25
2312186-4	1990	Thus, the serine protease specific for Boc-Gln-Gly-Arg-MCA in mite cultures of D. farinae was purified by ammonium sulfate precipitation and chromatographies on p-aminobenzamidine-sepharose CL-4B, DEAE-Toyopearl 650M, Sephadex G-75 superfine and Sephacryl S-200.	Sepharose CL 4B	180-195	coagulation factor II, thrombin	Homo sapiens	10-25
2788497-8	1989	From conditioned, serum-free medium of HSG-SF cells, an EGF-like molecule (Mr 46,000) was purified by using an anti-human EGF antibody-coupled Sepharose CL-4B column.	Sepharose CL 4B	143-158	epidermal growth factor	Homo sapiens	56-59
2600425-7	1989	Depending on the matrix used, the IgG3 eluted from the protein A columns exhibited a contamination of 1.8-88 ppm (weight/weight) protein A. Amongst the gels with IgG3 capacities greater than 10 mg/ml, the least contamination with protein A was observed in the IgG3 fractions from immobilized rProtein A and Protein A-Sepharose CL-4B (Fermentech).	Sepharose CL 4B	317-332	immunoglobulin heavy constant gamma 3 (G3m marker)	Homo sapiens	34-38
2466553-4	1989	CAR-3 antigen was purified by a three-step procedure, consisting of perchloric acid extraction, molecular sieving on Sepharose CL-4B, and affinity chromatography on AR-3 antibodies coupled to Sepharose 4B.	Sepharose CL 4B	117-132	carbonic anhydrase 3	Homo sapiens	0-5
2656675-6	1989	TR-1 was refractory to a second incubation with trypsin but could be digested by papain or Pronase to a smaller mucin glycopeptide fraction, as judged by the slight decrease in apparent molecular weight on Sepharose CL-4B.	Sepharose CL 4B	206-221	taste 1 receptor member 1	Homo sapiens	0-4
2656675-8	1989	The remainder of the radioactivity from papain or Pronase digests of TR-1 eluted, like the TR-3 fractions, in the salt fraction on Sepharose CL-4B.	Sepharose CL 4B	131-146	taste 1 receptor member 1	Homo sapiens	69-73
2656675-8	1989	The remainder of the radioactivity from papain or Pronase digests of TR-1 eluted, like the TR-3 fractions, in the salt fraction on Sepharose CL-4B.	Sepharose CL 4B	131-146	nuclear receptor subfamily 4 group A member 1	Homo sapiens	91-95
2668261-5	1989	Rabbit platelet phospholipase A2 released in vitro was purified by column chromatography using Sepharose CL-4B conjugated with anti-rat platelet derived phospholipase A2 monoclonal antibody, followed by reversed-phase HPLC.	Sepharose CL 4B	95-110	phospholipase A2	Oryctolagus cuniculus	16-32
2651564-3	1989	beta-NGF-specific antibody isolated on a column of Sepharose CL-4B coupled with purified beta-NGF reacted only with beta-NGF.	Sepharose CL 4B	51-66	nerve growth factor	Mus musculus	0-8
2495325-6	1989	Similarly, polyclonal IgM fractionated on a SPA-Sepharose CL4B column showed nearly complete partition of VHIII molecules into the SPA-binding fraction, and VHI and VHII subgroup proteins into the fall-through.	Sepharose CL 4B	48-62	surfactant protein A1	Homo sapiens	44-47
2538547-9	1989	To analyze the binding of gp120/160 to CD4, we used a molecular assay in which an mAb (110-4) coupled to Sepharose CL4B allowed the attachment of soluble gp120/160 to the beads; 125I-sCD4 was then added to measure the binding of CD4 to different amounts of gp120/160.	Sepharose CL 4B	105-119	stearoyl-CoA desaturase 5	Homo sapiens	183-187
2538547-9	1989	To analyze the binding of gp120/160 to CD4, we used a molecular assay in which an mAb (110-4) coupled to Sepharose CL4B allowed the attachment of soluble gp120/160 to the beads; 125I-sCD4 was then added to measure the binding of CD4 to different amounts of gp120/160.	Sepharose CL 4B	105-119	inter-alpha-trypsin inhibitor heavy chain 4	Homo sapiens	26-35
2538547-9	1989	To analyze the binding of gp120/160 to CD4, we used a molecular assay in which an mAb (110-4) coupled to Sepharose CL4B allowed the attachment of soluble gp120/160 to the beads; 125I-sCD4 was then added to measure the binding of CD4 to different amounts of gp120/160.	Sepharose CL 4B	105-119	CD4 molecule	Homo sapiens	39-42
2538547-9	1989	To analyze the binding of gp120/160 to CD4, we used a molecular assay in which an mAb (110-4) coupled to Sepharose CL4B allowed the attachment of soluble gp120/160 to the beads; 125I-sCD4 was then added to measure the binding of CD4 to different amounts of gp120/160.	Sepharose CL 4B	105-119	inter-alpha-trypsin inhibitor heavy chain 4	Homo sapiens	154-163
2538547-9	1989	To analyze the binding of gp120/160 to CD4, we used a molecular assay in which an mAb (110-4) coupled to Sepharose CL4B allowed the attachment of soluble gp120/160 to the beads; 125I-sCD4 was then added to measure the binding of CD4 to different amounts of gp120/160.	Sepharose CL 4B	105-119	inter-alpha-trypsin inhibitor heavy chain 4	Homo sapiens	154-163
3141586-3	1988	The solubilized P400 protein was purified using Sepharose CL-4B and Con A-Sepharose chromatography.	Sepharose CL 4B	48-63	E1A binding protein p400	Mus musculus	16-20
2541759-1	1989	Myosin was purified from bovine erythrocytes by chromatography on DEAE-cellulose, Sepharose CL-4B, hydroxylapatite, and DEAE-5PW.	Sepharose CL 4B	82-97	myosin heavy chain 14	Homo sapiens	0-6
2450875-6	1988	This major HSPG fraction was purified by ion-exchange chromatography on Mono Q and by gel filtration on Sepharose CL-4B, and further characterized by gel electrophoresis and immunoblotting.	Sepharose CL 4B	104-119	CD44 molecule (Indian blood group)	Homo sapiens	11-15
2833740-1	1988	Monoclonal antibodies against the porcine 1,25-dihydroxyvitamin D3 receptor were immobilized on Sepharose CL-4B and used to obtain a highly purified 1,25-dihydroxyvitamin D3 receptor fraction with a 45% recovery of the 1,25-dihydroxyvitamin D3 binding capacity.	Sepharose CL 4B	96-111	vitamin D receptor	Rattus norvegicus	42-75
7254200-3	1981	Chicken cell surface fibronectin from fibroblast cultures was purified by ammonium sulfate precipitation followed by chromatography on Sepharose-CL4B.	Sepharose CL 4B	135-149	fibronectin 1	Gallus gallus	21-32
2991442-1	1985	Varicella-zoster virus glycoprotein gp1/gp3 was purified by affinity chromatography using anti-gp1/gp3 monoclonal antibody 19.1 linked to CNBr-activated Sepharose CL-4B.	Sepharose CL 4B	153-168	GTP binding protein 1	Homo sapiens	36-39
2991442-1	1985	Varicella-zoster virus glycoprotein gp1/gp3 was purified by affinity chromatography using anti-gp1/gp3 monoclonal antibody 19.1 linked to CNBr-activated Sepharose CL-4B.	Sepharose CL 4B	153-168	GTP binding protein 1	Homo sapiens	95-98
3860177-1	1985	Insulin receptor was purified in high yield from cultured 3T3-L1 mouse adipocytes using the bifunctional ligand N alpha B1-(biotinyl-epsilon-aminocaproyl)insulin in conjunction with avidin-Sepharose CL-4B.	Sepharose CL 4B	189-204	insulin receptor	Mus musculus	0-16
6743226-2	1984	Human thyroglobulin (Tg) could be adsorbed through one of its thyroxine (T4) residues by either of two T4-binding antibodies which had been covalently attached to Sepharose- CL4B .	Sepharose CL 4B	163-178	thyroglobulin	Homo sapiens	6-19
6196237-3	1983	Immunoglobulin G (IgG) fraction thus prepared was covalently attached on a Sepharose CL-4B and used as an immunoadsorbent of serum TBG.	Sepharose CL 4B	75-90	serpin family A member 7	Homo sapiens	131-134
7129626-2	1982	In this study, we isolated infectious immunoglobulin G (IgG)-LDV complexes in the plasma of persistently infected mice by adsorption to and elution from protein A-Sepharose CL-4B.	Sepharose CL 4B	163-178	immunoglobulin heavy variable V1-62	Mus musculus	56-59
7103942-2	1982	Antibody from ascitic fluid was purified and coupled to Sepharose-CL-4B to give a specific affinity column, which was used to isolate C3b inactivator from human serum in 70% yield.	Sepharose CL 4B	56-71	complement factor I	Homo sapiens	134-149
7074033-2	1982	Mucin was solubilized in buffer and fractionated on Sepharose CL-4B, followed by CsBr density gradient centrifugation of the void volume fraction.	Sepharose CL 4B	52-67	LOC100508689	Homo sapiens	0-5
3222246-2	1988	The mucin was eluted at the void volume of Sepharose CL-4B and was of density greater than 1.3 in CsCl gradients.	Sepharose CL 4B	43-58	LOC100508689	Homo sapiens	4-9
2960860-1	1987	We have investigated the function of C3b receptor (CR1) in the malignant lymphocytes of B-chronic lymphocytic leukemia (B-CLL) mimicking the physiological ligand C3b with the anti-CR1 monoclonal antibody CB04 covalently linked to Sepharose CL-4B (CB04-S).	Sepharose CL 4B	230-245	complement C3b/C4b receptor 1 (Knops blood group)	Homo sapiens	51-54
3105105-5	1986	When incubated with VIII/vWF for 30 min at 37 degrees C and applied to Sepharose CL-4B, the hematin eluted with the VIII/vWF in the void volume.	Sepharose CL 4B	71-86	cytochrome c oxidase subunit 8A	Homo sapiens	20-24
3105105-5	1986	When incubated with VIII/vWF for 30 min at 37 degrees C and applied to Sepharose CL-4B, the hematin eluted with the VIII/vWF in the void volume.	Sepharose CL 4B	71-86	von Willebrand factor	Homo sapiens	25-28
3105105-5	1986	When incubated with VIII/vWF for 30 min at 37 degrees C and applied to Sepharose CL-4B, the hematin eluted with the VIII/vWF in the void volume.	Sepharose CL 4B	71-86	cytochrome c oxidase subunit 8A	Homo sapiens	116-120
3105105-5	1986	When incubated with VIII/vWF for 30 min at 37 degrees C and applied to Sepharose CL-4B, the hematin eluted with the VIII/vWF in the void volume.	Sepharose CL 4B	71-86	von Willebrand factor	Homo sapiens	121-124
3530729-3	1986	This monoclonal antibody seems to belong to IgG2b as it was eluted from protein A-Sepharose CL-4B with citrate buffer pH 3.5. competitive binding experiment using fragment peptides of LHRH indicated the binding site of LRH13 was a region around serine and tyrosine, and modification of mammalian LHRH by radioiodination caused a marked decrease in the binding activity.	Sepharose CL 4B	82-97	gonadotropin releasing hormone 1	Homo sapiens	184-188
3530729-3	1986	This monoclonal antibody seems to belong to IgG2b as it was eluted from protein A-Sepharose CL-4B with citrate buffer pH 3.5. competitive binding experiment using fragment peptides of LHRH indicated the binding site of LRH13 was a region around serine and tyrosine, and modification of mammalian LHRH by radioiodination caused a marked decrease in the binding activity.	Sepharose CL 4B	82-97	gonadotropin releasing hormone 1	Homo sapiens	296-300
3491261-7	1986	In the purification reagent, Mac 003 is immobilised on sepharose CL-4B to purify recombinant IL-2 from less than 1% in an E. Coli extract, to greater than 90% purity, in a single step with greater than 80% yield.	Sepharose CL 4B	55-70	interleukin 2	Homo sapiens	93-97
6549133-2	1984	During isolation by gel exclusion chromatography on Sepharose CL-4B, the apoB forms mixed micelles of protein and detergent that are free of endogenous lipids.	Sepharose CL 4B	52-67	apolipoprotein B	Homo sapiens	73-77
6226659-2	1983	Purified AP-2 covalently coupled to Sepharose CL4B was used as an immunoadsorbent column to purify the IIb-IIIa complex from a preparation of Triton X-100-solubilized human platelet proteins.	Sepharose CL 4B	36-50	transcription factor AP-2 alpha	Homo sapiens	9-13
6403520-4	1983	The first protein, C-reactive protein, passes through the Sepharose CL-4B column in the presence of Ca2+ whereas the second protein, serum amyloid P component, remains bound.	Sepharose CL 4B	58-73	C-reactive protein	Oryctolagus cuniculus	19-37
6165740-3	1981	Gel filtration of Sepharose CL-4B revealed that aliquots of urine collected 1 hour after injection contained polymer fragments of HES 350/0.60 with values of Kav ranging between 0.88 and 0.84, and possessed a Stokes radius (r = 32A) similar to that of Dextran 20 (M-W 22 700).	Sepharose CL 4B	18-33	ribosome binding protein 1	Homo sapiens	130-133
352577-2	1978	After the procedure of optimization, human serum albumin was assayed using anti-human serum albumin bound to Sepharose CL 4-B in the enzyme thermistor unit and catalase as label on the free antigen.	Sepharose CL 4B	109-125	albumin	Homo sapiens	43-56
352577-2	1978	After the procedure of optimization, human serum albumin was assayed using anti-human serum albumin bound to Sepharose CL 4-B in the enzyme thermistor unit and catalase as label on the free antigen.	Sepharose CL 4B	109-125	albumin	Homo sapiens	86-99
25278441-2	2014	In this study, we established a novel method for isolating Abeta-interacting proteins by utilizing regular comb polymer immobilized on Sepharose CL-4B.	Sepharose CL 4B	135-150	amyloid beta (A4) precursor protein	Mus musculus	59-64
6266723-2	1981	A covalently bound to Sepharose CL-4B was used to enrich sera in the IgG3 subclass of antibodies.	Sepharose CL 4B	22-37	immunoglobulin heavy constant gamma 3 (G3m marker)	Homo sapiens	69-73
508810-4	1979	A reductive alkylation treatment of the unpurified mucin fraction followed by Sepharose CL-4B chromatography removed contaminating protein and most of the mannose-containing material from the mucin fraction.	Sepharose CL 4B	78-93	solute carrier family 13 member 2	Rattus norvegicus	192-197
29545735-3	2018	Methods: The SAP was extracted and purified using Sepharose CL-4B gel from S. aspratus.	Sepharose CL 4B	50-65	SH2 domain containing 1A	Homo sapiens	13-16