PMID-sentid	Pub_year	Sent_text	compound_name	comp_offset	prot_official_name	organism	prot_offset
8804622-1	1996	We have employed two independent techniques to measure the intracellular pH (pHi) in giant glial cells of the leech Hirudo medicinalis, using the fluorescent dye 2",7"-bis-(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF) and double-barreled neutral-carrier, pH-sensitive microelectrodes, which also record the membrane potential.	bcecf	213-218	glucose-6-phosphate isomerase	Bos taurus	77-80
9030742-1	1997	Murine and human cell lines overexpressing the multidrug-resistance protein (MRP) showed a marked decreased accumulation of the fluorescent dye 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF).	bcecf	195-200	ATP binding cassette subfamily C member 1	Homo sapiens	47-75
9030742-1	1997	Murine and human cell lines overexpressing the multidrug-resistance protein (MRP) showed a marked decreased accumulation of the fluorescent dye 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF).	bcecf	195-200	ATP binding cassette subfamily C member 1	Homo sapiens	77-80
9030742-4	1997	Decreased accumulation was linked to active efflux of the hydrophilic free acid form of BCECF from the MRP-overexpressing cell lines, indicating that dye extrusion occurs after the dye ester has been converted to the free acid form in the cytoplasm.	bcecf	88-93	ATP-binding cassette, sub-family C (CFTR/MRP), member 1	Mus musculus	103-106
8914588-1	1996	2",7"-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) is frequently used for fluorometric determination of intracellular pH (pHi) and its metabolic changes.	bcecf	57-62	glucose-6-phosphate isomerase	Homo sapiens	135-138
8914588-2	1996	Studies of BCECF-loaded platelets have reported different pHi values in the range of 6.98 to 7.35, despite the use of the same probe.	bcecf	11-16	glucose-6-phosphate isomerase	Homo sapiens	58-61
8914588-6	1996	When least affected by BCECF, platelet pHi is 7.34.	bcecf	23-28	glucose-6-phosphate isomerase	Homo sapiens	39-42
8945628-3	1996	Intracellular pH (pH(i)) of PC12 cells was measured at 37 degrees C by microfluorimetric analysis of 2",7"-bis-(2-carboxyethyl)-5(and -6)-carboxyfluorescein (BCECF).	bcecf	158-163	glucose-6-phosphate isomerase	Rattus norvegicus	18-23
7485599-3	1995	pHi was measured with the pH-sensitive fluorescent dye 2",7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF).	bcecf	105-110	glucose-6-phosphate isomerase	Oryctolagus cuniculus	0-3
8544767-5	1996	NHE activity was determined fluorometrically with 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF).	bcecf	101-106	solute carrier family 9 member C1	Homo sapiens	0-3
7880851-4	1995	Continuous monitoring of intracellular pH (pHi) in BCECF (2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein)-loaded Caco-2 cell monolayers indicated that apical addition of MeAIB (20 mM) was associated with H(+)-flow across the apical membrane in both Na+ and Na(+)-free conditions.	bcecf	51-56	glucose-6-phosphate isomerase	Homo sapiens	43-46
1479478-1	1992	A new flow cytometric method was developed for measuring the intracellular pH (pHi) of mammalian cells using a fluorescent pH indicator dye 2",7-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF).	bcecf	195-200	glucose-6-phosphate isomerase	Homo sapiens	79-82
7736501-3	1995	pHi was measured in cells loaded with the pH sensitive fluorescent dye, 2",7"-bis-(2-carboxyethyl)-5-(and 6)-carboxyfluorescein (BCECF).	bcecf	129-134	glucose-6-phosphate isomerase	Rattus norvegicus	0-3
7760347-2	1995	Developed tension (DT), intracellular pH (pHi) with the pH-sensitive dye 2"-7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF) and resting membrane potential (Vm) with 3M KCl filled glass microelectrodes were measured.	bcecf	123-128	glucose-6-phosphate isomerase	Homo sapiens	42-45
8178955-2	1994	In the nominal absence of CO2-HCO3-, each agent increased pHi, measured with 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF).	bcecf	128-133	glucose-6-phosphate isomerase	Rattus norvegicus	58-61
7927433-3	1994	The activity of the Na/H antiport was determined by a new technique which involves the measurement of the fluorescence of 2",7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF) loaded rbc"s as function of their intracellular pH (pHi).	bcecf	172-177	glucose-6-phosphate isomerase	Rattus norvegicus	231-234
1295678-1	1992	Microspectrofluorometry was used to study the regulation of intracellular pH (pHi) in 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF)-loaded astrocytes and the neuroblastoma-glioma cells of the NG 108-15 line.	bcecf	137-142	glucose-6-phosphate isomerase 1	Mus musculus	78-81
20732098-3	1992	In contrast, the efflux of 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) was much greater, ranging from 45% in HGT-1 cells to 76% in HCT-8 and T84 cells over 5 hr.	bcecf	78-83	solute carrier family 25 member 16	Homo sapiens	123-128
1733231-1	1992	The fluorescent pH-sensitive dye 2",7"-bis(2-carboxyethyl)-5(6)- carboxyfluorescein (BCECF) was used to determine changes in intracellular pH (pHi) associated with activation of secretion in isolated cells from the salt-secreting avian nasal gland.	bcecf	85-90	glucose-6-phosphate isomerase	Homo sapiens	143-146
1945761-1	1991	The pH-sensitive dye 2",7"-bis(2-carboxyethyl)-5(and -6)-carboxyfluorescein (BCECF) was used to measure intracellular pH (pHi) in rat mesenteric resistance arteries (internal diameter 152-289 microns) mounted in an isometric myograph for simultaneous measurements of force.	bcecf	77-82	glucose-6-phosphate isomerase	Rattus norvegicus	122-125
1900792-9	1991	In single parietal cells double-labeled with fura-2 and BCECF, a temporal decrease in pHi preceded the rise in Cai after stimulation with nigericin.	bcecf	56-61	glucose-6-phosphate isomerase	Homo sapiens	86-89
2058696-2	1991	pHi was monitored using the fluorescent pH-sensitive dye 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF).	bcecf	108-113	glucose-6-phosphate isomerase	Rattus norvegicus	0-3
2058696-2	1991	pHi was monitored using the fluorescent pH-sensitive dye 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF).	bcecf	108-113	glucose-6-phosphate isomerase	Rattus norvegicus	0-2
1846269-1	1991	We used the fluorescent dye 2",7"-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) to examine intracellular pH (pHi) regulation in single hepatocytes isolated from control rats and rats with either spontaneous or drug-induced diabetes mellitus (DM).	bcecf	80-85	glucose-6-phosphate isomerase	Rattus norvegicus	116-119
2382707-1	1990	A dual-excitation inverted confocal laser-scanning microscope has been developed for measuring intracellular pH (pHi) using 2",7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF) in individual cells in the isolated perfused cortical collecting tubule (CCT).	bcecf	174-179	glucose-6-phosphate isomerase	Homo sapiens	113-116
1660575-3	1991	Measurements were made using the pH-sensitive fluorescent dye bis (carboxyethyl) carboxyfluorescein (BCECF).	bcecf	101-106	glucose-6-phosphate isomerase	Homo sapiens	33-35
2169197-1	1990	The intracellular pH (pHi) dependence of the rate of Na(+)-H+ exchange was determined in undifferentiated promyelocytic HL-60 cells by measuring alkalinization rates using the fluorescent pHi indicator 2",7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF).	bcecf	252-257	glucose-6-phosphate isomerase	Homo sapiens	22-25
2166591-3	1990	While stimulation of platelets loaded with the fluorescent intracellular pH-sensitive dye, bis-carboxyethyl-5-(6)-carboxyfluorescein (BCECF) with thrombin resulted in the activation of the Na+/H+ exchanger, activation of this antiport did not occur in collagen-stimulated platelets.	bcecf	134-139	coagulation factor II, thrombin	Homo sapiens	146-154
2153709-3	1990	Intracellular pH (pHi) was measured fluorometrically using the pH-sensitive dye (2",7")-bis (carboxyethyl)-(5,6)-carboxyfluorescein (BCECF).	bcecf	133-138	glucose-6-phosphate isomerase	Oryctolagus cuniculus	18-21
2158745-2	1990	Regulation of pHi was fluorometrically monitored using the pH-sensitive probe, 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF).	bcecf	130-135	glucose-6-phosphate isomerase	Macaca fascicularis	14-17
2180402-1	1990	The effect of endothelin (ET) on the intracellular pH (pHi) of vascular smooth muscle cells (VSMC), was investigated using a fluorescent pH indicator 2",7"-bis(carboxyethyl)carboxyfluorescein (BCECF).	bcecf	193-198	glucose-6-phosphate isomerase	Homo sapiens	55-58
2455669-1	1988	Mechanisms of intracellular pH (pHi) regulation were investigated using the pH-sensitive dye BCECF (2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein) to measure pHi in isolated antral cells from Necturus, rabbit, and guinea pig.	bcecf	93-98	glucose-6-phosphate isomerase	Oryctolagus cuniculus	32-35
2548385-1	1989	The intracellular pH (pHi) in ventricular myocytes isolated from adult rat heart was measured in suspension using the pH-sensitive dye 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF).	bcecf	186-191	glucose-6-phosphate isomerase	Rattus norvegicus	22-25
2493743-2	1989	Intracellular pH (pHi) was measured using the fluorescent dye 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescin (BCECF).	bcecf	112-117	glucose-6-phosphate isomerase	Homo sapiens	18-21
24295212-3	2014	Intracellular pH (pHi) was determined using 2",7"-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) and whole spectrum analysis.	bcecf	101-106	glucose-6-phosphate isomerase	Homo sapiens	18-21
2455669-2	1988	The BCECF fluorescence was calibrated in terms of pHi by using a nigericin-high potassium concentration method to equilibrate pHi and pHo.	bcecf	4-9	glucose-6-phosphate isomerase	Oryctolagus cuniculus	50-53
2455669-2	1988	The BCECF fluorescence was calibrated in terms of pHi by using a nigericin-high potassium concentration method to equilibrate pHi and pHo.	bcecf	4-9	glucose-6-phosphate isomerase	Oryctolagus cuniculus	126-129
3599651-4	1987	In the present studies, the pH-sensitive fluorescent dye, bis-(carboxyethyl)-carboxyfluorescein (BCECF) has been used to study pHi changes in suspensions of canine proximal tubule cells following acidification or alkalinization of the cytosol.	bcecf	97-102	glucose-6-phosphate isomerase	Homo sapiens	127-130
27630987-6	2016	The intracellular pH (pHi) measured using the fluorescent ratio dye 2",7"-bis(2-carboxyethyl)-5(6)-155 carboxyfluorescein (BCECF) revealed expected differences between normal and cancer cells (low and high, respectively), and a mixed distribution in the MINO cells, with a subset of cells in the MINO having an increased rate of acidification when proton efflux was inhibited.	bcecf	123-128	glucose-6-phosphate isomerase	Homo sapiens	18-20
27630987-6	2016	The intracellular pH (pHi) measured using the fluorescent ratio dye 2",7"-bis(2-carboxyethyl)-5(6)-155 carboxyfluorescein (BCECF) revealed expected differences between normal and cancer cells (low and high, respectively), and a mixed distribution in the MINO cells, with a subset of cells in the MINO having an increased rate of acidification when proton efflux was inhibited.	bcecf	123-128	glucose-6-phosphate isomerase	Homo sapiens	22-25
25241983-5	2014	We therefore investigated the mechanism of pHi recovery from intracellular acidosis and alkalosis, induced by NH4Cl-prepulse and Na-acetate-prepulse, respectively, using intracellular 2",7"-bis(2-carboxethyl)-5(6)- carboxy-fluorescein (BCECF)-fluorescence in HRASMCs.	bcecf	236-241	glucose-6-phosphate isomerase	Homo sapiens	43-46
21116627-5	2011	The cellular accumulation of a specific MRP5 fluorescent substrate 2",7"-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) into these cells was measured by flow cytometry and the cell proliferation determined by a 72-h CyQuant assay.	bcecf	118-123	ATP binding cassette subfamily C member 5	Homo sapiens	40-44
23548792-6	2013	NHE3 transcript levels and protein abundance in intestinal tissue were determined by confocal immunofluorescent microscopy, RT-PCR and western blotting, cytosolic pH (pHi) in intestinal cells from 2",7"-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) fluorescence and Na(+)/H(+) exchanger activity from the Na(+) dependent realkalinization following an ammonium pulse.	bcecf	254-259	solute carrier family 9 (sodium/hydrogen exchanger), member 3	Mus musculus	0-4
21116627-6	2011	RESULTS: The cellular accumulation of BCECF in HEK293/MRP5 cells and in PANC-1 and MiaPaCa-2 cells was significantly increased by curcumin in a concentration-dependent manner.	bcecf	38-43	ATP binding cassette subfamily C member 5	Homo sapiens	54-58
15097861-4	2004	Commonly, pHi is measured using the fluorescent dye biscarboxyethylcarboxyfluorescein (BCECF).	bcecf	87-92	glucose-6-phosphate isomerase	Homo sapiens	10-13
19745779-5	2009	Intracellular pH (pHi) was measured by microfluorometry in cells loaded with 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF).	bcecf	128-133	glucose-6-phosphate isomerase	Homo sapiens	18-21
19479940-6	2009	The functional activity of NHE isoforms was measured microfluorometrically using a pH-sensitive fluorescent dye, 2",7"-bis(carbonylethyl)-5(6)-carboxyfluorescein (BCECF), and a NHE-inhibitor, 3-methylsulfonyl-4-piperidinobenzoyl guanidine methanesulfonate (HOE694).	bcecf	163-168	solute carrier family 9 member C1	Homo sapiens	27-30
19050954-4	2009	In this work, pHi was determined using the pH-sensitive fluorescent probe 2",7"-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF).	bcecf	131-136	glucose-6-phosphate isomerase	Bos taurus	14-17
18022597-6	2007	The pHi was measured with the pH-sensitive fluorescent dye bis-carboxyethyl carboxy-fluorescein (BCECF).	bcecf	97-102	glucose-6-phosphate isomerase	Homo sapiens	4-7
16564041-7	2006	Intracellular pH (pHi) was measured using the pH-sensitive fluorescent probe bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF)-AM.	bcecf	123-128	glucose-6-phosphate isomerase	Homo sapiens	18-21
16554372-3	2006	P-gp transport kinetics were determined indirectly by calculating the difference in BCECF accumulation when P-gp was functional and completely inhibited by GF120918 (3.2 microM).	bcecf	84-89	phosphoglycolate phosphatase	Homo sapiens	0-4
16554372-3	2006	P-gp transport kinetics were determined indirectly by calculating the difference in BCECF accumulation when P-gp was functional and completely inhibited by GF120918 (3.2 microM).	bcecf	84-89	phosphoglycolate phosphatase	Homo sapiens	108-112
16554372-7	2006	Like P-gp, the Vmax of BCECF for MRP-related transport was overwhelmingly higher in the BBM compared with the cultured cells.	bcecf	23-28	phosphoglycolate phosphatase	Homo sapiens	5-9
16554372-7	2006	Like P-gp, the Vmax of BCECF for MRP-related transport was overwhelmingly higher in the BBM compared with the cultured cells.	bcecf	23-28	ATP binding cassette subfamily C member 3	Homo sapiens	33-36
15498800-8	2004	Cl(-)-HCO(3)(-) and Cl(-)-OH(-) exchange activity of SLC26A6 and AE3 were investigated in transfected HEK293 cells, using intracellular fluorescence measurements of 2",7"-bis (2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF), to monitor intracellular pH (pH(i)).	bcecf	217-222	solute carrier family 26 member 6	Homo sapiens	53-60
15097861-6	2004	Our results indicate that BCECF fluorescence is not only dependent on pHi but also on the total fluorescence intensity of the detected area (which may be influenced by dye loading, dye leakage, and the shutter size on the photomultiplier).	bcecf	26-31	glucose-6-phosphate isomerase	Homo sapiens	70-73
12879156-1	2003	We employed human red blood cells as a model system to check the affinity of MRP1 (Multidrug Resistance-associated Protein 1) towards fluorescein and a set of its carboxyl derivatives: 5/6-carboxyfluorescein (CF), 2",7"-bis-(2-carboxyethyl)-5/6-carboxyfluorescein (BCECF) and calcein (CAL).	bcecf	265-270	ATP binding cassette subfamily C member 1	Homo sapiens	77-81
12879156-1	2003	We employed human red blood cells as a model system to check the affinity of MRP1 (Multidrug Resistance-associated Protein 1) towards fluorescein and a set of its carboxyl derivatives: 5/6-carboxyfluorescein (CF), 2",7"-bis-(2-carboxyethyl)-5/6-carboxyfluorescein (BCECF) and calcein (CAL).	bcecf	265-270	ATP binding cassette subfamily C member 1	Homo sapiens	83-124
12879156-6	2003	BCECF is transported mainly via MRP1, as proven by the use of QCRL3, a monoclonal antibody known to specifically inhibit MRP1-mediated transport.	bcecf	0-5	ATP binding cassette subfamily C member 1	Homo sapiens	32-36
12879156-6	2003	BCECF is transported mainly via MRP1, as proven by the use of QCRL3, a monoclonal antibody known to specifically inhibit MRP1-mediated transport.	bcecf	0-5	ATP binding cassette subfamily C member 1	Homo sapiens	121-125
12598410-12	2003	Experiments with BCECF [2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein]-loaded Caco-2 cells demonstrate that VIP reduces the NHE3-dependent recovery of intracellular pH (pH(i)) after dipeptide-induced acidification.	bcecf	17-22	vasoactive intestinal peptide	Homo sapiens	112-115
12598410-12	2003	Experiments with BCECF [2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein]-loaded Caco-2 cells demonstrate that VIP reduces the NHE3-dependent recovery of intracellular pH (pH(i)) after dipeptide-induced acidification.	bcecf	17-22	solute carrier family 9 member A3	Homo sapiens	128-132
11810216-2	2002	The pH-sensitive fluorescent dye 2",7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF) was used to measure pHi.	bcecf	83-88	glucose-6-phosphate isomerase	Rattus norvegicus	4-6
10578014-21	1999	Use of the pH-sensitive dye 2"7" bis-(2-carboxyethyl)-5(and 6) carboxyfluorescein (BCECF) suggested that the final intracellular pH (pH(i)) was approximately 6.9, as though pH(i) was largely determined by endogenous cellular regulation.	bcecf	83-88	glucose-6-phosphate isomerase	Cricetulus griseus	133-138
10578014-21	1999	Use of the pH-sensitive dye 2"7" bis-(2-carboxyethyl)-5(and 6) carboxyfluorescein (BCECF) suggested that the final intracellular pH (pH(i)) was approximately 6.9, as though pH(i) was largely determined by endogenous cellular regulation.	bcecf	83-88	glucose-6-phosphate isomerase	Cricetulus griseus	173-178
10362747-3	1999	pHi was measured with the pH-sensitive fluorescent dye 2",7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF) under resting conditions and in response to NH4Cl pulse.	bcecf	105-110	glucose-6-phosphate isomerase	Homo sapiens	0-3
9890750-7	1999	The steady-state pHi was then determined by spectrofluorometric measurement of bis(carboxyethyl)5(6)-carboxyfluoroscein (BCECF) fluorescence.	bcecf	121-126	glucose-6-phosphate isomerase	Homo sapiens	17-20
9512007-4	1998	Coadministration of Fe2+ and ascorbate (Fe-Asc) did not affect DCF fluorescence, but highly reduced the intracellular pH (pHi = 6.32 +/- 0.08), in comparison with control conditions (pHi = 7.05 +/- 0.11), as determined with the probe BCECF (2",7"-bis-(carboxyethyl)-5(and-6) carboxyfluorescein).	bcecf	234-239	PYD and CARD domain containing	Homo sapiens	43-46
9447934-3	1997	The type of transporter was identified in functional studies by monitoring uptake of monocarboxylates into SMC through measurement of the cytosolic pH (pHi) with the pH-sensitive dye 2",7"-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF).	bcecf	235-240	glucose-6-phosphate isomerase	Homo sapiens	152-155
9530271-3	1998	Intracellular pH (pHi) was determined by the use of the fluorescent pH-sensitive dye, 2",7"-bis(2- carboxyethyl)-5(6)-carboxyfluorescein (BCECF).	bcecf	138-143	glucose-6-phosphate isomerase	Homo sapiens	18-21
9401966-6	1997	Many of the experimental manipulations produced a decrease in intracellular pH (pHi) measured in sister cultures using the pH-sensitive dye 2",7"-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF).	bcecf	197-202	glucose-6-phosphate isomerase	Rattus norvegicus	80-83
9160715-4	1997	pH(i) was measured using BCECF (2",7"-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein) in freshly ejaculated sperm (T0 sperm) and in sperm incubated in vitro overnight (T24 sperm).	bcecf	25-30	glucose-6-phosphate isomerase	Homo sapiens	0-5