PMID-sentid Pub_year Sent_text compound_name comp_offset prot_official_name organism prot_offset 8731356-3 1996 In the present study we determined whether the response to carbachol also involves AT1 receptors. Carbachol 59-68 angiotensin II receptor, type 1a Rattus norvegicus 83-86 8731356-6 1996 The AT1 receptor antagonist DUP-753 (50 nmol/microliters) injected into the LV reduced water intake induced by ANG II (10 nmol/microliters) from 9.2 +/- 1.4 to 0.4 +/- 0.1 ml/h (N = 8), and water intake induced by carbachol (2 nmol/microliters) from 9.8 +/- 1.4 ml/h to 3.7 +/- 0.8 ml/h (N = 8). Carbachol 214-223 angiotensin II receptor, type 1a Rattus norvegicus 4-7 8566594-0 1996 Epidermal growth factor inhibits carbachol-stimulated canine parietal cell function via protein kinase C. BACKGROUND & AIMS: Epidermal growth factor (EGF) inhibits secretagogue-stimulated gastric acid secretion via an EGF receptor located on parietal cells. Carbachol 33-42 epidermal growth factor Canis lupus familiaris 0-23 8566594-0 1996 Epidermal growth factor inhibits carbachol-stimulated canine parietal cell function via protein kinase C. BACKGROUND & AIMS: Epidermal growth factor (EGF) inhibits secretagogue-stimulated gastric acid secretion via an EGF receptor located on parietal cells. Carbachol 33-42 epidermal growth factor Canis lupus familiaris 129-152 8566594-0 1996 Epidermal growth factor inhibits carbachol-stimulated canine parietal cell function via protein kinase C. BACKGROUND & AIMS: Epidermal growth factor (EGF) inhibits secretagogue-stimulated gastric acid secretion via an EGF receptor located on parietal cells. Carbachol 33-42 epidermal growth factor Canis lupus familiaris 154-157 8566594-0 1996 Epidermal growth factor inhibits carbachol-stimulated canine parietal cell function via protein kinase C. BACKGROUND & AIMS: Epidermal growth factor (EGF) inhibits secretagogue-stimulated gastric acid secretion via an EGF receptor located on parietal cells. Carbachol 33-42 epidermal growth factor Canis lupus familiaris 222-225 8566594-5 1996 RESULTS: EGF dose dependently inhibited carbachol-stimulated aminopyrine uptake in a pertussis toxin-insensitive, genistein (tyrosine kinase inhibitor)--sensitive manner, with a maximal inhibitory effect (37.5% +/- 6.8%) achieved at 10(-7) mol/L. Carbachol 40-49 epidermal growth factor Canis lupus familiaris 9-12 8566594-10 1996 CONCLUSIONS: The inhibitory action of EGF on carbachol-stimulated parietal cell activity seems to involve protein kinase C. Carbachol 45-54 epidermal growth factor Canis lupus familiaris 38-41 8596502-0 1996 Glucagon-like peptide-1 stimulates insulin secretion but not phosphoinositide hydrolysis from islets desensitized by prior exposure to high glucose or the muscarinic agonist carbachol. Carbachol 174-183 glucagon Homo sapiens 0-23 8596502-2 1996 Compared with responses observed from control islets incubated for 3.5 hours with 5.6 mmol/L glucose alone, prior exposure to 10 mmol/L glucose, 20 mmol/L glucose, or 10 micromol/L carbachol reduced peak second-phase insulin release rates to a subsequent 20-mmol/L glucose stimulus by 63%, 81%, or 70%, respectively. Carbachol 181-190 insulin Homo sapiens 217-224 8596502-5 1996 Carbachol (10 micromol/L) preexposure also abolished the subsequent insulin secretory and 3H-inositol efflux responses to 8 mmol/L glucose plus 10 micromol/L carbachol. Carbachol 0-9 insulin Homo sapiens 68-75 8596502-5 1996 Carbachol (10 micromol/L) preexposure also abolished the subsequent insulin secretory and 3H-inositol efflux responses to 8 mmol/L glucose plus 10 micromol/L carbachol. Carbachol 158-167 insulin Homo sapiens 68-75 8599027-4 1996 Muscarinic stimulation with carbachol (CCh) induced a cytosolic acidification (0.15 +/- 0.01 pH unit after 5 min). Carbachol 28-37 glucose-6-phosphate isomerase Rattus norvegicus 93-95 8599027-4 1996 Muscarinic stimulation with carbachol (CCh) induced a cytosolic acidification (0.15 +/- 0.01 pH unit after 5 min). Carbachol 39-42 glucose-6-phosphate isomerase Rattus norvegicus 93-95 8599027-5 1996 Cu2+ enhanced the CCh-stimulated acidification 2-fold (0.30 +/- 0.02 pH unit after 5 min). Carbachol 18-21 glucose-6-phosphate isomerase Rattus norvegicus 69-71 8573085-0 1996 Asymmetric signal transduction in polarized ileal Na(+)-absorbing cells: carbachol activates brush-border but not basolateral-membrane PIP2-PLC and translocates PLC-gamma 1 only to the brush border. Carbachol 73-82 phospholipase C gamma 1 Homo sapiens 161-172 8573085-7 1996 Western analysis and immunoprecipitation demonstrated that PLC-gamma 1 is present in the brush border and that carbachol increases the PLC-gamma 1 amount in the brush border. Carbachol 111-120 phospholipase C gamma 1 Homo sapiens 135-146 8573085-12 1996 These studies demonstrate that carbachol but not Ca2+ ionophore effects on brush-border NaCl absorption are associated with increases in brush-border but not BLM PIP2-specific PLC activity and in the amount of brush-border PLC-gamma 1, and involve tyrosine phosphorylation. Carbachol 31-40 phospholipase C gamma 1 Homo sapiens 223-234 8786004-1 1996 Smooth muscle cells isolated from cecal circular smooth muscle of the guinea pig were used to determine whether thyrotropin-releasing hormone (TRH) can inhibit the contractile response produced by 10(-6) M carbachol by exerting a direct action on muscle cells. Carbachol 206-215 thyrotropin releasing hormone Cavia porcellus 112-141 8786004-1 1996 Smooth muscle cells isolated from cecal circular smooth muscle of the guinea pig were used to determine whether thyrotropin-releasing hormone (TRH) can inhibit the contractile response produced by 10(-6) M carbachol by exerting a direct action on muscle cells. Carbachol 206-215 thyrotropin releasing hormone Cavia porcellus 143-146 8786004-3 1996 TRH inhibited the contractile response produced by 10(-6) M carbachol in a concentration-dependent manner, with an IC50 value of 4 nM, 2",5"-Dideoxyadenosine and phorbol 12-myristate 13-acetate did not have any significant effect on the TRH-induced relaxation. Carbachol 60-69 thyrotropin releasing hormone Cavia porcellus 0-3 8786004-3 1996 TRH inhibited the contractile response produced by 10(-6) M carbachol in a concentration-dependent manner, with an IC50 value of 4 nM, 2",5"-Dideoxyadenosine and phorbol 12-myristate 13-acetate did not have any significant effect on the TRH-induced relaxation. Carbachol 60-69 thyrotropin releasing hormone Cavia porcellus 237-240 8682156-5 1996 The addition of vasoactive intestinal peptide (VIP; 10(-9) to 10(-7) mol L(-1)) or of histamine (10(-5) to 10(-3) mol L(-1)) increased PAF production by three- to fivefold, while the addition of carbachol (10(-7) to 10(-4) mol L(-1)) increased PAF production up to sevenfold. Carbachol 195-204 vasoactive intestinal peptide Homo sapiens 16-45 8682156-8 1996 This is the first report showing PAF production by gastric epithelial cells in response to histamine, VIP and carbachol. Carbachol 110-119 PCNA clamp associated factor Homo sapiens 33-36 8713465-4 1996 During intracellular recordings from CA1 pyramidal cells in hippocampal slices from rats, the cholinergic agonist carbachol caused several reversible changes in the action potential: low doses (2 microM) caused an increase in spike duration; high doses (10-40 microM) or long-lasting applications also reduced the spike amplitude and rate of rise, and raised the spike threshold. Carbachol 114-123 carbonic anhydrase 1 Rattus norvegicus 37-40 8801525-0 1996 Change in subcellular localization of gastrin-like immunoreactivity in epithelial cells of rat duodenum induced by carbachol. Carbachol 115-124 gastrin Rattus norvegicus 38-45 8801525-6 1996 However, in carbachol-stimulated animals, immunogold labeling as well as DAB reactions were accumulated at the apical portion of the cytoplasm, suggesting that a high concentration of gastrin was involved in the apical cytoplasm. Carbachol 12-21 gastrin Rattus norvegicus 184-191 8801525-9 1996 These findings suggest that the gastrin-like immunoreactivity was pooled at the matrix of apical cytoplasm in carbachol-stimulated cells, which might be derived from the secretory granules migrated from the basal cytoplasm into apical portion of the cells. Carbachol 110-119 gastrin Rattus norvegicus 32-39 8801525-10 1996 In conclusion, the present study demonstrated the change in subcellular localization of gastrin-like immunoreactivity in intestinal gastrin cells after stimulation with carbachol. Carbachol 169-178 gastrin Rattus norvegicus 88-95 8801525-10 1996 In conclusion, the present study demonstrated the change in subcellular localization of gastrin-like immunoreactivity in intestinal gastrin cells after stimulation with carbachol. Carbachol 169-178 gastrin Rattus norvegicus 132-139 9259051-3 1996 We have now found in rat aorta that carbachol, a muscarinic cholinergic agonist that promotes release of nitric oxide (NO), inhibits expression of c-fos and c-jun mRNA induced by alpha 1 receptors. Carbachol 36-45 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 147-152 9259051-4 1996 NO synthase inhibitors blocked the effects of carbachol on c-fos mRNA and a cGMP analog mimicked carbachol. Carbachol 46-55 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 59-64 8788942-15 1995 The rate of myoblast fusion was increased by carbachol, an effect antagonized by alpha-bungarotoxin, curare and decamethonium, but not by atropine, indicating that nAChR were involved. Carbachol 45-54 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 164-169 8974845-15 1995 These results indicate that PGE2 receptor EP2 subtype, but not PGF2 alpha receptor, is involved in the inhibition (hence relaxation by inference) of carbachol-induced porcine ciliary muscle cell contraction. Carbachol 149-158 prostaglandin E receptor 2 Homo sapiens 42-45 8745167-6 1995 Tandospirone suppressed carbachol-stimulated phosphatidyl-inositol metabolism (PI response), which was shown to be a 5-HT1A receptor-mediated event. Carbachol 24-33 5-hydroxytryptamine receptor 1A Rattus norvegicus 117-123 8847629-11 1995 In the presence of La3+, the caffeine response in the guinea-pig ureter and carbachol response in the rat ureter, elicited in Ca(2+)-free solutions, were always increased and prolonged and could be repeatedly evoked, suggesting similarity in Ca2+ uptake behaviour of the store in both species. Carbachol 76-85 carbonic anhydrase 2 Rattus norvegicus 242-245 8847629-14 1995 However, ryanodine failed to prevent the rat ureter responding to carbachol, suggesting that carbachol was releasing Ca2+ from a ryanodine-insensitive channel in the sarcoplasmic reticulum (SR). Carbachol 93-102 carbonic anhydrase 2 Rattus norvegicus 117-120 7492301-7 1995 In addition, regulated mucin secretion by LS180 cells was studied in response to carbachol, phorbol 12-myristate 13-acetate and A23187. Carbachol 81-90 LOC100508689 Homo sapiens 23-28 7592809-7 1995 Xenopus oocytes injected with M2 muscarinic receptor (M2) plus either GIRK2 or CIR cRNA expressed only very small carbachol-induced currents, while co-injection of CIR plus GIRK2 along with M2 resulted in expression of carbachol-activated strong inwardly rectifying currents. Carbachol 219-228 corepressor interacting with RBPJ, 1 L homeolog Xenopus laevis 164-167 7592840-10 1995 Fetal calf serum, phenylephrine, and carbachol were less potent activators of c-Raf and A-Raf. Carbachol 37-46 Raf-1 proto-oncogene, serine/threonine kinase Rattus norvegicus 78-83 7592840-10 1995 Fetal calf serum, phenylephrine, and carbachol were less potent activators of c-Raf and A-Raf. Carbachol 37-46 A-Raf proto-oncogene, serine/threonine kinase Rattus norvegicus 88-93 7592809-7 1995 Xenopus oocytes injected with M2 muscarinic receptor (M2) plus either GIRK2 or CIR cRNA expressed only very small carbachol-induced currents, while co-injection of CIR plus GIRK2 along with M2 resulted in expression of carbachol-activated strong inwardly rectifying currents. Carbachol 219-228 potassium inwardly rectifying channel subfamily J member 6 Homo sapiens 173-178 7595517-4 1995 The dose dependency for carbachol stimulation of PtdIns synthase and phospholipase C was similar (EC50 approximately 20 microM) as was the relative intrinsic activity of muscarinic receptor partial agonists. Carbachol 24-33 CDP-diacylglycerol--inositol 3-phosphatidyltransferase Homo sapiens 49-64 7491942-6 1995 These data led to the following conclusions: 1) PACAP was more potent than either carbachol or VIP in enhancing the secretion of catecholamine from the adrenal medulla, and 2) PACAP-induced catecholamine secretion was due to the direct action of PACAP on the adrenal medulla rather than an indirect action mediated by acetylcholine release. Carbachol 82-91 adenylate cyclase activating polypeptide 1 Rattus norvegicus 176-181 7491942-6 1995 These data led to the following conclusions: 1) PACAP was more potent than either carbachol or VIP in enhancing the secretion of catecholamine from the adrenal medulla, and 2) PACAP-induced catecholamine secretion was due to the direct action of PACAP on the adrenal medulla rather than an indirect action mediated by acetylcholine release. Carbachol 82-91 adenylate cyclase activating polypeptide 1 Rattus norvegicus 176-181 8654498-6 1995 The magnitude of the contraction caused by 10(-8) M endothelin-1 was about 30% in the longitudinal muscle strips and 29% in the circular muscle strips, relative to the contraction caused by 10(-5) M carbachol, a muscarinic agonist. Carbachol 199-208 endothelin 1 Bos taurus 52-64 7595498-3 1995 Pretreatment of cells with a maximal effective concentration (5 microM) of BK did not affect the subsequent carbachol (CCh)-induced [Ca2+]i rise, but CCh (1 mM) pretreatment completely abolished the BK-induced [Ca2+]i rise without inhibition of BK-induced inositol 1,4,5-trisphosphate (IP3) generation. Carbachol 150-153 kininogen 1 Homo sapiens 199-201 7595498-3 1995 Pretreatment of cells with a maximal effective concentration (5 microM) of BK did not affect the subsequent carbachol (CCh)-induced [Ca2+]i rise, but CCh (1 mM) pretreatment completely abolished the BK-induced [Ca2+]i rise without inhibition of BK-induced inositol 1,4,5-trisphosphate (IP3) generation. Carbachol 150-153 kininogen 1 Homo sapiens 199-201 7595498-5 1995 However, the addition of atropine at plateau phases of CCh-induced [Ca2+]i rise and IP3 production caused a rapid decline to the basal levels and then restored the [Ca2+]i rise by BK. Carbachol 55-58 kininogen 1 Homo sapiens 180-182 8868061-4 1995 The nAChR single channel properties were investigated in the presence of carbachol (1 microM) in the pipette. Carbachol 73-82 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 4-9 7557075-7 1995 ANP and VIP inhibited 1 mumol/L carbachol-induced contraction in a dose-dependent manner. Carbachol 32-41 VIP peptides Cavia porcellus 8-11 8751075-5 1995 In the carbachol-precontracted guinea-pig taenia coli, reactive red 2 (0.1 to 10 microM) shifted the concentration-response curve for the P2Y-purinoceptor-mediated relaxation effect of adenosine 5"-O-(2-thiodiphosphate) (ADP beta S) progressively to the right; only at the highest concentration of antagonist (10 microM) was the maximum slightly depressed; a pA2 value of 7.55 (Kd 0.028 microM) was derived from the shift. Carbachol 7-16 adenosine deaminase, RNA-specific, B2 Rattus norvegicus 64-69 7557088-9 1995 [Ca2+]i chelator and calmodulin antagonist inhibited the carbachol-induced pepsinogen secretion and NO generation. Carbachol 57-66 calmodulin 1 Homo sapiens 21-31 8569054-2 1995 Carbachol stimulated the release of histamine and the activity of HDC with 30-50% the intensity of the maximal effect of the antigen. Carbachol 0-9 histidine decarboxylase Homo sapiens 66-69 7476883-0 1995 Phosphorylation of cyclic AMP response element-binding protein and induction of c-fos gene expression on withdrawal from chronic treatment with carbachol in NG108-15 cells. Carbachol 144-153 FBJ osteosarcoma oncogene Mus musculus 80-85 7476883-6 1995 In cells treated with carbachol for 48 hr, induction of withdrawal with the muscarinic antagonist atropine led to a small increase in intracellular cAMP concentration but an 11.6-fold increase in the phosphorylation of CREB and a 3.4-fold increase in accumulation of c-fos mRNA. Carbachol 22-31 cAMP responsive element binding protein 1 Mus musculus 219-223 7476883-6 1995 In cells treated with carbachol for 48 hr, induction of withdrawal with the muscarinic antagonist atropine led to a small increase in intracellular cAMP concentration but an 11.6-fold increase in the phosphorylation of CREB and a 3.4-fold increase in accumulation of c-fos mRNA. Carbachol 22-31 FBJ osteosarcoma oncogene Mus musculus 267-272 7476883-7 1995 The adenylyl cyclase inhibitor 2",5"-dideoxyadenosine, which attenuated the chronic carbachol-induced increase in cAMP concentration, prevented the increased phosphorylation of CREB and the enhanced accumulation of c-fos mRNA during atropine-induced withdrawal. Carbachol 84-93 cAMP responsive element binding protein 1 Mus musculus 177-181 7476883-7 1995 The adenylyl cyclase inhibitor 2",5"-dideoxyadenosine, which attenuated the chronic carbachol-induced increase in cAMP concentration, prevented the increased phosphorylation of CREB and the enhanced accumulation of c-fos mRNA during atropine-induced withdrawal. Carbachol 84-93 FBJ osteosarcoma oncogene Mus musculus 215-220 8570026-4 1995 Carbachol-induced responses were antagonized by both atropine and mecamylamine in a manner consistent with agonist effects of carbachol at both nicotinic and muscarinic sites, while concentration-effect curves to DMPP and muscarine were shifted rightward in a parallel manner by mecamylamine (10 microM) and atropine (5 nM), with antagonist pKB estimates of 6.4 and 9.0, respectively. Carbachol 0-9 AKT serine/threonine kinase 1 Homo sapiens 341-344 7578678-1 1995 In the present study, we have determined the influence of tumor necrosis factor alpha (TNF alpha) on both basal and carbamylcholine chloride (Cch)-induced [Ca2+]i in granulosa cells from the largest (F1) and smallest (F5,6) preovulatory follicles. Carbachol 116-140 tumor necrosis factor Homo sapiens 58-85 7578678-1 1995 In the present study, we have determined the influence of tumor necrosis factor alpha (TNF alpha) on both basal and carbamylcholine chloride (Cch)-induced [Ca2+]i in granulosa cells from the largest (F1) and smallest (F5,6) preovulatory follicles. Carbachol 116-140 tumor necrosis factor Homo sapiens 87-96 7578678-1 1995 In the present study, we have determined the influence of tumor necrosis factor alpha (TNF alpha) on both basal and carbamylcholine chloride (Cch)-induced [Ca2+]i in granulosa cells from the largest (F1) and smallest (F5,6) preovulatory follicles. Carbachol 142-145 tumor necrosis factor Homo sapiens 58-85 7578678-1 1995 In the present study, we have determined the influence of tumor necrosis factor alpha (TNF alpha) on both basal and carbamylcholine chloride (Cch)-induced [Ca2+]i in granulosa cells from the largest (F1) and smallest (F5,6) preovulatory follicles. Carbachol 142-145 tumor necrosis factor Homo sapiens 87-96 7578678-6 1995 Pretreatment with TNF alpha (4-5 min) increased the magnitude of the Cch response in both F1 and F5,6 granulosa cells previously incapable of producing large Cch-induced changes in [Ca2+]i. Carbachol 69-72 tumor necrosis factor Homo sapiens 18-27 7578678-6 1995 Pretreatment with TNF alpha (4-5 min) increased the magnitude of the Cch response in both F1 and F5,6 granulosa cells previously incapable of producing large Cch-induced changes in [Ca2+]i. Carbachol 158-161 tumor necrosis factor Homo sapiens 18-27 7578678-7 1995 In F1 cells, the effects of TNF alpha on Cch-induced [Ca2+]i were far more extensive, such that the Cch response in the presence of TNF alpha was indistinguishable from the fast Cch-induced Ca2+ transient reported previously. Carbachol 41-44 tumor necrosis factor Homo sapiens 28-37 7578678-7 1995 In F1 cells, the effects of TNF alpha on Cch-induced [Ca2+]i were far more extensive, such that the Cch response in the presence of TNF alpha was indistinguishable from the fast Cch-induced Ca2+ transient reported previously. Carbachol 41-44 tumor necrosis factor Homo sapiens 132-141 7578678-7 1995 In F1 cells, the effects of TNF alpha on Cch-induced [Ca2+]i were far more extensive, such that the Cch response in the presence of TNF alpha was indistinguishable from the fast Cch-induced Ca2+ transient reported previously. Carbachol 100-103 tumor necrosis factor Homo sapiens 28-37 7578678-7 1995 In F1 cells, the effects of TNF alpha on Cch-induced [Ca2+]i were far more extensive, such that the Cch response in the presence of TNF alpha was indistinguishable from the fast Cch-induced Ca2+ transient reported previously. Carbachol 100-103 tumor necrosis factor Homo sapiens 132-141 7578678-7 1995 In F1 cells, the effects of TNF alpha on Cch-induced [Ca2+]i were far more extensive, such that the Cch response in the presence of TNF alpha was indistinguishable from the fast Cch-induced Ca2+ transient reported previously. Carbachol 100-103 tumor necrosis factor Homo sapiens 28-37 7578678-7 1995 In F1 cells, the effects of TNF alpha on Cch-induced [Ca2+]i were far more extensive, such that the Cch response in the presence of TNF alpha was indistinguishable from the fast Cch-induced Ca2+ transient reported previously. Carbachol 100-103 tumor necrosis factor Homo sapiens 132-141 7578678-8 1995 Furthermore, the TNF alpha effect was reversible, as subsequent challenge with Cch in the absence of TNF alpha failed to produce the large Ca(2+)-transients observed earlier with the cytokine present. Carbachol 79-82 tumor necrosis factor Homo sapiens 17-26 8637618-0 1995 The association of thirst, sodium appetite and vasopressin release with c-fos expression in the forebrain of the rat after intracerebroventricular injection of angiotensin II, angiotensin-(1-7) or carbachol. Carbachol 197-206 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 72-77 8637618-1 1995 The effect intracerebroventricular injections of angiotensin II (0.1 nm), angiotensin-(1-7) (1 or 100 nm) and carbachol (500 ng) on c-fos expression was examined in the forebrain of Lister hooded rats. Carbachol 110-119 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 132-137 8637618-14 1995 Angiotensin II and carbachol caused an approximate five-fold increase in plasma vasopressin levels compared to cerebrospinal fluid-injected rats, but angiotensin-(1-7) had no effect on vasopressin release. Carbachol 19-28 arginine vasopressin Rattus norvegicus 80-91 8788591-5 1995 Carbachol increased the concentration and the transcription rate of alpha Gi1-mRNA and [1,4,5]IP3R-mRNA both in neonatal and adult samples. Carbachol 0-9 inositol 1,4,5-trisphosphate receptor, type 1 Rattus norvegicus 94-98 8788591-10 1995 Carbachol increases the steady-state concentration of both alpha Gi1-mRNA and [1,4,5]IP3R-mRNA possibly by altering their synthesis and stability in brain. Carbachol 0-9 inositol 1,4,5-trisphosphate receptor, type 1 Rattus norvegicus 85-89 7578678-10 1995 In addition, TNF alpha appeared to enhance Cch-induced mobilization of Ca2+ from intracellular stores. Carbachol 43-46 tumor necrosis factor Homo sapiens 13-22 7654194-1 1995 Chinese hamster ovary cells stably transfected with human M3 muscarinic acetylcholine receptors show a 40-50% reduction in the immunoreactive G-proteins Gq alpha and G11 alpha when stimulated with the cholinergic agonist carbachol. Carbachol 221-230 cholinergic receptor muscarinic 3 Homo sapiens 58-95 7654194-3 1995 The effect is specific for Gq alpha family proteins as Gs alpha was slightly increased after carbachol treatment and G13 alpha was unchanged. Carbachol 93-102 GNAS complex locus Homo sapiens 55-63 7557261-0 1995 Carbachol-induced desensitization of rat basophilic leukemia (RBL-2H3) cells transfected with human m3 muscarinic acetylcholine receptors. Carbachol 0-9 cholinergic receptor muscarinic 3 Homo sapiens 100-137 7477901-0 1995 Fos and serotonin immunoreactivity in the raphe nuclei of the cat during carbachol-induced active sleep: a double-labeling study. Carbachol 73-82 Fos proto-oncogene, AP-1 transcription factor subunit Felis catus 0-3 8570018-0 1995 Activation of 5-HT1A receptors inhibits carbachol-stimulated inositol 1,4,5-trisphosphate mass accumulation in the rodent hippocampus. Carbachol 40-49 5-hydroxytryptamine receptor 1A Rattus norvegicus 14-20 8570018-1 1995 We have previously demonstrated that 5-HT1A receptor agonists partially prevent the stimulation by carbachol of [3H]-phosphoinositide hydrolysis in immature rat hippocampal slices. Carbachol 99-108 5-hydroxytryptamine receptor 1A Rattus norvegicus 37-43 8570018-3 1995 In hippocampal slices from developing rats and in hippocampal neurons, carbachol enhanced the accumulation of IP3 and this response was partially inhibited by 8-OH-DPAT with a potency compatible with the affinity of this agonist for 5-HT1A receptors. Carbachol 71-80 5-hydroxytryptamine receptor 1A Rattus norvegicus 233-239 7501705-5 1995 In the current study, we investigated the effect of Mg2+ on the contraction and intracellular free calcium of rabbit urinary bladder detrusor muscle in response to carbachol and transmural field stimulation (FS). Carbachol 164-173 mucin 7, secreted Homo sapiens 52-55 7650010-1 1995 mAChR-stimulated PLD was turned off after 2 min of receptor activation with either the full (carbachol) or partial agonist (pilocarpine) and remained completely suppressed for at least 4 h. Partial recovery was observed 24 h after agonist removal. Carbachol 93-102 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 17-20 8584207-5 1995 Microinfusion of muscarinic agonist, carbachol, induced Fos expression as well, but mostly in the oxytocin neurons. Carbachol 37-46 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 56-59 7653652-9 1995 These data provide the first demonstration that adenylyl cyclase within the mPRF contributes to the carbachol induction of REM sleep and respiratory depression. Carbachol 100-109 Spi-C transcription factor (Spi-1/PU.1 related) Mus musculus 76-80 7590625-2 1995 TRH inhibited carbachol (10(-5) M)-stimulated amylase secretion by a maximum of 24% at a concentration of 10(-11) M (p < 0.05), but did not affect basal amylase release in concentrations from 10(-13) M to 10(-8) M. Ceruletide (3 x 10(-10) M)-stimulated amylase secretion was maximally reduced by 23% at a TRH concentration of 10(-10) M (p < 0.05). Carbachol 14-23 thyrotropin releasing hormone Rattus norvegicus 0-3 7590625-2 1995 TRH inhibited carbachol (10(-5) M)-stimulated amylase secretion by a maximum of 24% at a concentration of 10(-11) M (p < 0.05), but did not affect basal amylase release in concentrations from 10(-13) M to 10(-8) M. Ceruletide (3 x 10(-10) M)-stimulated amylase secretion was maximally reduced by 23% at a TRH concentration of 10(-10) M (p < 0.05). Carbachol 14-23 thyrotropin releasing hormone Rattus norvegicus 308-311 7482289-0 1995 Corticosteroid-mediated modulation of carbachol responsiveness in CA1 pyramidal neurons: a voltage clamp analysis. Carbachol 38-47 carbonic anhydrase 1 Rattus norvegicus 66-69 7625999-1 1995 Carbachol stimulation of the muscarinic acetylcholine m1 receptor (m1R), stably expressed in Rat 1a fibroblasts, resulted in a calcium-dependent activation of c-Jun kinase (JNK). Carbachol 0-9 mitogen-activated protein kinase 8 Rattus norvegicus 173-176 7626007-4 1995 Activation of MAPK and MAPK kinase (MEK) by carbachol returned to control levels within 30-60 min, whereas activation by FCS was more sustained. Carbachol 44-53 mitogen activated protein kinase 3 Rattus norvegicus 14-18 7626007-4 1995 Activation of MAPK and MAPK kinase (MEK) by carbachol returned to control levels within 30-60 min, whereas activation by FCS was more sustained. Carbachol 44-53 mitogen activated protein kinase 3 Rattus norvegicus 23-27 7626007-5 1995 FPLC on Mono Q showed that carbachol and FCS activated two peaks of MEK and two peaks of MAPK (p42MAPK and p44MAPK). Carbachol 27-36 mitogen activated protein kinase 3 Rattus norvegicus 89-93 7626007-5 1995 FPLC on Mono Q showed that carbachol and FCS activated two peaks of MEK and two peaks of MAPK (p42MAPK and p44MAPK). Carbachol 27-36 mitogen activated protein kinase 1 Rattus norvegicus 95-102 7626007-5 1995 FPLC on Mono Q showed that carbachol and FCS activated two peaks of MEK and two peaks of MAPK (p42MAPK and p44MAPK). Carbachol 27-36 mitogen activated protein kinase 3 Rattus norvegicus 107-114 7626007-6 1995 Pretreatment of cells with PTX for 24 h inhibited the activation of MAPK by carbachol, FCS and lysophosphatidic acid, but not that by endothelin-1, phenylephrine or isoprenaline. Carbachol 76-85 mitogen activated protein kinase 3 Rattus norvegicus 68-72 7583249-3 1995 Surprisingly, superfusion or local application of low concentrations of acetylcholine (ACh), carbachol (CCh) or muscarine reduced the amplitudes of CA1 field potentials evoked by stratum radiatum (SR) stimulation. Carbachol 93-102 carbonic anhydrase 1 Rattus norvegicus 148-151 7583249-3 1995 Surprisingly, superfusion or local application of low concentrations of acetylcholine (ACh), carbachol (CCh) or muscarine reduced the amplitudes of CA1 field potentials evoked by stratum radiatum (SR) stimulation. Carbachol 104-107 carbonic anhydrase 1 Rattus norvegicus 148-151 7587302-0 1995 Effect of carbachol on phospholipase C-mediated phosphatidylinositol 4,5-bisphosphate hydrolysis, and its modulation by isoproterenol in rabbit corneal epithelial cells. Carbachol 10-19 LOC100009319 Oryctolagus cuniculus 23-38 7587302-1 1995 The effects of carbachol (CCh) on phospholipase C(PLC)-mediated phosphatidylinositol 4,5-bisphosphate (PIP2) hydrolysis and its modulation by isoproterenol were investigated in SV40-adenovirus transformed rabbit corneal epithelial cells (RCEC). Carbachol 15-24 LOC100009319 Oryctolagus cuniculus 34-49 7587302-1 1995 The effects of carbachol (CCh) on phospholipase C(PLC)-mediated phosphatidylinositol 4,5-bisphosphate (PIP2) hydrolysis and its modulation by isoproterenol were investigated in SV40-adenovirus transformed rabbit corneal epithelial cells (RCEC). Carbachol 26-29 LOC100009319 Oryctolagus cuniculus 34-49 7494138-5 1995 Preincubation of the SAN cells with L-nitro-arginine methyl ester (L-NAME; 0.2-1 mM), which inhibits NO synthase (NOS), abolished the CCh-induced attenuation of ICa(L) but had no effect on IK(ACh). Carbachol 134-137 nitric oxide synthase 2 Homo sapiens 101-112 8567506-7 1995 Subsequently, we found that sheep developed airway hyperresponsiveness to inhaled carbachol at 4 and 24 h after ET-1 challenge, an effect that was blocked by aerosolized BQ-123. Carbachol 82-91 endothelin-1 Ovis aries 112-116 7478930-0 1995 Role of calmodulin in the activation of carbachol-activated cationic current in guinea-pig gastric antral myocytes. Carbachol 40-49 calmodulin-2 Cavia porcellus 8-18 7478930-10 1995 These data suggest that multiple Ca2+-dependent pathways are involved in the activation of CCh-gated cation channels in guinea-pig antral myocytes and a Ca2+/calmodulin-dependent pathway would be one of them. Carbachol 91-94 calmodulin-2 Cavia porcellus 158-168 7662698-4 1995 Heavy staining with the antibody for autophosphorylated CaMK II was observed in the cytoplasm of chief cells treated with carbamylcholine chloride or ionomycin, but only light staining was seen in cells treated with TPA or forskolin. Carbachol 122-146 calcium/calmodulin dependent protein kinase II gamma Homo sapiens 56-63 8528696-3 1995 As 5-HT, GABA-, and CCh-stimulated high-affinity GTPase activities are mediated by the 5-HT1A, GABAB, and pirenzepine-insensitive muscarinic receptors, respectively, the additive effects indicate that these three receptors are independently coupled to distinct pools of G proteins. Carbachol 20-23 5-hydroxytryptamine receptor 1A Rattus norvegicus 87-93 7625999-1 1995 Carbachol stimulation of the muscarinic acetylcholine m1 receptor (m1R), stably expressed in Rat 1a fibroblasts, resulted in a calcium-dependent activation of c-Jun kinase (JNK). Carbachol 0-9 mitogen-activated protein kinase 8 Rattus norvegicus 159-171 7477901-3 1995 Compared with control cats, which were injected with saline, active sleep-carbachol cats exhibited a significantly greater number of c-fos-expressing neurons in the raphe dorsalis, magnus and pallidus. Carbachol 74-83 Fos proto-oncogene, AP-1 transcription factor subunit Felis catus 133-138 7611446-4 1995 We observed that, like in the glomerulus, endothelin and PAF induced, in the parietal sheet, [Ca2+]i responses that differed in many respects from those found with carbachol. Carbachol 164-173 PCNA clamp associated factor Rattus norvegicus 57-60 7477901-6 1995 These data indicate that there is an increased number of non-serotonergic, c-fos-expressing neurons in the raphe dorsalis, magnus and pallidus during the carbachol-induced state. Carbachol 154-163 Fos proto-oncogene, AP-1 transcription factor subunit Felis catus 75-80 7539427-0 1995 Carbachol, substance P, and phorbol ester promote the tyrosine phosphorylation of protein kinase C delta in salivary gland epithelial cells. Carbachol 0-9 protein kinase C delta Homo sapiens 82-104 7539427-2 1995 In response to substance P and the muscarinic agonist carbachol, two ligands that activate phospholipase C-linked receptors, which stimulate fluid secretion, PKC delta was phosphorylated on tyrosine residues. Carbachol 54-63 protein kinase C delta Homo sapiens 158-167 7494138-7 1995 In the presence of ISO the CCh-induced inhibition of ICa(L) could be mimicked by the NO donor 3-morpholino-sydnonimine (SIN-1; 0.1 mM). Carbachol 27-30 MAPK associated protein 1 Homo sapiens 120-125 7611446-2 1995 The aim of the present work was to investigate whether this structure could be also a target of endothelin and platelet-activating factor (PAF), since we observed [Ca2+]i increases in response to both agonists in the glomerulus, but which were very different from that induced by carbachol. Carbachol 280-289 PCNA clamp associated factor Rattus norvegicus 111-137 7611446-2 1995 The aim of the present work was to investigate whether this structure could be also a target of endothelin and platelet-activating factor (PAF), since we observed [Ca2+]i increases in response to both agonists in the glomerulus, but which were very different from that induced by carbachol. Carbachol 280-289 PCNA clamp associated factor Rattus norvegicus 139-142 7611446-5 1995 Thus, in the presence of 2 mM external calcium, 1) endothelin and PAF responses spontaneously declined to basal level, whereas a stationary plateau was observed after a sharp peak of [Ca2+]i with carbachol; 2) the magnitude of [Ca2+]i peak was smaller with endothelin and PAF than with carbachol; and 3) endothelin and PAF, but not carbachol, induced a homologous dose-dependent desensitization. Carbachol 286-295 PCNA clamp associated factor Rattus norvegicus 66-69 7611446-5 1995 Thus, in the presence of 2 mM external calcium, 1) endothelin and PAF responses spontaneously declined to basal level, whereas a stationary plateau was observed after a sharp peak of [Ca2+]i with carbachol; 2) the magnitude of [Ca2+]i peak was smaller with endothelin and PAF than with carbachol; and 3) endothelin and PAF, but not carbachol, induced a homologous dose-dependent desensitization. Carbachol 286-295 PCNA clamp associated factor Rattus norvegicus 66-69 7751925-6 1995 Compared to vehicle- and carbachol-treated animals without REMc, the animals with REMc showed a significantly higher number of Fos-LI cells in pontine regions that have been implicated in REM sleep generation. Carbachol 25-34 Fos proto-oncogene, AP-1 transcription factor subunit Felis catus 127-130 7730640-3 1995 Stimulation of these cells with Ag, carbachol, Ca(2+)-ionophore, or thapsigargin resulted in the phosphorylation of Raf1, MEK1, p42mapk MAP kinase, and the recently cloned cytosolic phospholipase A2 (PLA2) and increased activities of both MAP kinase and PLA2, as well as release of arachidonic acid. Carbachol 36-45 Raf-1 proto-oncogene, serine/threonine kinase Rattus norvegicus 116-120 7730640-3 1995 Stimulation of these cells with Ag, carbachol, Ca(2+)-ionophore, or thapsigargin resulted in the phosphorylation of Raf1, MEK1, p42mapk MAP kinase, and the recently cloned cytosolic phospholipase A2 (PLA2) and increased activities of both MAP kinase and PLA2, as well as release of arachidonic acid. Carbachol 36-45 mitogen activated protein kinase kinase 1 Rattus norvegicus 122-126 7730640-3 1995 Stimulation of these cells with Ag, carbachol, Ca(2+)-ionophore, or thapsigargin resulted in the phosphorylation of Raf1, MEK1, p42mapk MAP kinase, and the recently cloned cytosolic phospholipase A2 (PLA2) and increased activities of both MAP kinase and PLA2, as well as release of arachidonic acid. Carbachol 36-45 phospholipase A2 group IVA Rattus norvegicus 172-198 7721739-5 1995 In contrast, in cells transfected with M1mAChRs, which effectively couple to Gq/PLC, carbachol increased ANF reporter gene expression 10-fold and also increased ANF protein, as determined by immunofluorescence. Carbachol 85-94 natriuretic peptide A Homo sapiens 105-108 7721739-5 1995 In contrast, in cells transfected with M1mAChRs, which effectively couple to Gq/PLC, carbachol increased ANF reporter gene expression 10-fold and also increased ANF protein, as determined by immunofluorescence. Carbachol 85-94 natriuretic peptide A Homo sapiens 161-164 7721739-6 1995 Carbachol-mediated ANF gene expression was inhibited by the mAChR antagonist pirenzepine with a Ki value characteristic of an M1mAChR. Carbachol 0-9 natriuretic peptide A Homo sapiens 19-22 7609913-5 1995 We therefore tested the effects of the cholinergic agonists acetylcholine and carbachol on excised KCa channels. Carbachol 78-87 casein kappa Homo sapiens 99-102 7713942-2 1995 The effect is mimicked by phorbol esters, which directly activate protein kinase C. Using human embryonic kidney cells expressing individual muscarinic receptor subtypes, we found that stimulation of APPs release by the muscarinic agonist carbachol was only partially reduced by a specific inhibitor of protein kinase C (the bisindolylmaleimide GF 109203X), while the response to phorbol 12-myristate 13-acetate (PMA) was abolished. Carbachol 239-248 cathepsin B Homo sapiens 200-204 7713942-3 1995 The increase in APPs release elicited by carbachol and PMA was accompanied by elevated tyrosine phosphorylation of several proteins and reduced by tyrosine kinase inhibitors; GF 109203X significantly reduced the stimulation of tyrosine phosphorylation by carbachol and PMA. Carbachol 41-50 cathepsin B Homo sapiens 16-20 7713942-3 1995 The increase in APPs release elicited by carbachol and PMA was accompanied by elevated tyrosine phosphorylation of several proteins and reduced by tyrosine kinase inhibitors; GF 109203X significantly reduced the stimulation of tyrosine phosphorylation by carbachol and PMA. Carbachol 255-264 cathepsin B Homo sapiens 16-20 7713942-6 1995 The results implicate both tyrosine phosphorylation and protein kinase C-dependent mechanisms in the regulation of APPs release by G protein-coupled receptors, and suggest that carbachol and PMA increase APPs release from human embryonic kidney cells expressing m3 muscarinic receptors via partially divergent pathways that converge at a tyrosine phosphorylation-dependent step. Carbachol 177-186 cathepsin B Homo sapiens 115-119 7713942-6 1995 The results implicate both tyrosine phosphorylation and protein kinase C-dependent mechanisms in the regulation of APPs release by G protein-coupled receptors, and suggest that carbachol and PMA increase APPs release from human embryonic kidney cells expressing m3 muscarinic receptors via partially divergent pathways that converge at a tyrosine phosphorylation-dependent step. Carbachol 177-186 cathepsin B Homo sapiens 204-208 7891088-9 1995 Together, these results suggest that both the massive influx of calcium induced by NMDA and the coupling of muscarinic receptors with a putative phospholipase A2 are required for the strong synergistic effects of carbachol and NMDA on [3H]AA release. Carbachol 213-222 phospholipase A2 group IB Homo sapiens 145-161 7786304-4 1995 Carbachol (1 mM) and potassium (100 mM) caused a time (T1/2 = 3 and 4 min, respectively) and dose (EC50 = 6.95 microM and 34.7 mM respectively) related increase in cAMP formation. Carbachol 0-9 CD5 molecule Homo sapiens 55-69 7706286-2 1995 We had previously constructed two triple point alanine mutants of the i3 junction of the muscarinic Hm1 receptor, W209A/I211A/Y212A and E360A/K362A/T366A, which are defective in mediating carbachol stimulation of phosphatidylinositol (PI) turnover (Moro, O., Lameh, J., Hogger, P., and Sadee, W. (1993) J. Biol. Carbachol 188-197 cholinergic receptor muscarinic 1 Homo sapiens 100-103 7706902-8 1995 Repeated BK stimuli also led to partial desensitization (70% to 85%) to adenosine triphosphatase and carbachol (heterologous desensitization). Carbachol 101-110 kininogen 1 Canis lupus familiaris 9-11 7700249-4 1995 Maximal (1 mM) carbachol concentrations abolished the elevation of [Ca2+]i produced by bradykinin but the muscarinic antagonist atropine (10 microM) restored the response, provided that extracellular Ca2+ was present throughout the experiment or was added before bradykinin. Carbachol 15-24 kininogen 1 Homo sapiens 87-97 7700249-5 1995 Carbachol also abolished bradykinin-mediated Ins(1,4,5)P3 elevation. Carbachol 0-9 kininogen 1 Homo sapiens 25-35 7700249-8 1995 In these cells carbachol again abolished bradykinin-mediated elevation of [Ca2+]i but only attenuated, rather than abolished, the elevation of Ins(1,4,5)P3 levels. Carbachol 15-24 kininogen 1 Homo sapiens 41-51 7700249-10 1995 Thus, depletion of an intracellular Ins(1,4,5)P3-sensitive Ca2+ store may underlie the ability of carbachol to produce not only heterologous desensitization of the [Ca2+]i elevation induced by bradykinin but also that of the Ins(1,4,5)P3 response. Carbachol 98-107 kininogen 1 Homo sapiens 193-203 7493606-0 1995 Caffeine and carbachol act on common Ca2+ stores to release Ca2+ in guinea-pig ileal smooth muscle. Carbachol 13-22 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 37-40 7493606-0 1995 Caffeine and carbachol act on common Ca2+ stores to release Ca2+ in guinea-pig ileal smooth muscle. Carbachol 13-22 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 60-63 7493606-2 1995 Caffeine (20 mM), carbachol (10 or 100 microM) or IP3 (40 microM), applied after loading Ca2+ within intracellular stores, produced a transient rise in tension in a Ca(2+)-free solution. Carbachol 18-27 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 89-92 7493606-6 1995 The effects of carbachol and IP3 were abolished after combined treatment with ryanodine (30 microM) and caffeine (20 mM) which causes functional removal of caffeine-releasable Ca2+ stores, but not after combined treatment with ryanodine (30 microM) and carbachol (10 microM). Carbachol 15-24 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 176-179 7493606-7 1995 The results suggest that caffeine, carbachol and IP3 all act on common Ca2+ stores to release Ca2+. Carbachol 35-44 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 71-74 7493606-7 1995 The results suggest that caffeine, carbachol and IP3 all act on common Ca2+ stores to release Ca2+. Carbachol 35-44 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 94-97 7887929-5 1995 Insulin secretagogues (28 mM glucose + 0.5 mM carbachol) caused a rapid and transient increase in PtdIns(3,4,5)P3 levels which peaked at 2-5 min, corresponding to peak early phase insulin release. Carbachol 46-55 insulin Homo sapiens 0-7 7887929-5 1995 Insulin secretagogues (28 mM glucose + 0.5 mM carbachol) caused a rapid and transient increase in PtdIns(3,4,5)P3 levels which peaked at 2-5 min, corresponding to peak early phase insulin release. Carbachol 46-55 insulin Homo sapiens 180-187 7890682-14 1995 In particular, carbachol caused a much greater induction of c-jun mRNA and AP-1 activity. Carbachol 15-24 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 60-65 7876266-7 1995 Under these conditions, carbachol caused a time-dependent 2-fold increase in APP-S secretion into the medium. Carbachol 24-33 cathepsin B Homo sapiens 77-82 7876266-8 1995 In contrast, prolonged treatment with carbachol caused a decrease in A beta production into the medium. Carbachol 38-47 amyloid beta precursor protein Homo sapiens 69-75 7883044-6 1995 The contribution of the carbamylcholine moiety of the site-directed reducing agent was clearly demonstrated in kinetic studies where reduction abilities of ARA, DTT and the methylated analogue of ARA (MeRA) were compared. Carbachol 24-39 ATP binding cassette subfamily C member 6 Homo sapiens 156-159 7883044-6 1995 The contribution of the carbamylcholine moiety of the site-directed reducing agent was clearly demonstrated in kinetic studies where reduction abilities of ARA, DTT and the methylated analogue of ARA (MeRA) were compared. Carbachol 24-39 ATP binding cassette subfamily C member 6 Homo sapiens 196-199 7798903-3 1995 The neuroprotective action of 1S,3R-ACPD was prevented by the mGluR antagonist (RS)-alpha-methyl-4-carboxyphenylglycine (MCPG) and was mimicked by N-methyl-D-aspartate or carbamylcholine but not by agonists of the mGluR subtypes negatively linked to adenylyl cyclase. Carbachol 171-186 homer scaffold protein 2 Homo sapiens 36-40 7823957-5 1995 In two astrocytoma lines of human origin, CCF and 1321N1, ets-1 phosphorylation was stimulated by bradykinin and carbachol, respectively. Carbachol 113-122 ETS proto-oncogene 1, transcription factor Homo sapiens 58-63 7538684-3 1995 The possible costimulation of CGRP with SP, NKA or carbachol on 5-day-old and adult rats was also tested. Carbachol 51-60 calcitonin-related polypeptide alpha Rattus norvegicus 30-34 7832780-11 1995 Addition of carbachol to M5-Trpl cells at 30-36 h after infection produced a large increase in [Ca2+]i to a sustained value of 677 +/- 143 nM. Carbachol 12-21 transient receptor potential-like Drosophila melanogaster 28-32 7814389-9 1995 We show that in astrocytoma cells, okadaic acid and cyclosporin A (an inhibitor of calcineurin) both potentiate the Ca2+ elevations due to low doses of CIF, thapsigargin, or carbachol. Carbachol 174-183 protein phosphatase 3, catalytic subunit, alpha isozyme L homeolog Xenopus laevis 83-94 8580531-6 1995 Apparently, the inhibitory action of IL-2 on IFN gamma involves an impaired response or atria to cholinergic activation, as IL-2 shifts to the right the dose response curve of the tissue to the cholinergic muscarinic agonist carbachol. Carbachol 225-234 interleukin 2 Rattus norvegicus 37-41 8580531-6 1995 Apparently, the inhibitory action of IL-2 on IFN gamma involves an impaired response or atria to cholinergic activation, as IL-2 shifts to the right the dose response curve of the tissue to the cholinergic muscarinic agonist carbachol. Carbachol 225-234 interferon gamma Rattus norvegicus 45-54 8580531-6 1995 Apparently, the inhibitory action of IL-2 on IFN gamma involves an impaired response or atria to cholinergic activation, as IL-2 shifts to the right the dose response curve of the tissue to the cholinergic muscarinic agonist carbachol. Carbachol 225-234 interleukin 2 Rattus norvegicus 124-128 7840204-4 1995 The stimulatory effects of carbachol on goblet cell degranulation in isolated pancreatic ducts were blocked by atropine and enhanced by simultaneous exposure to VIP. Carbachol 27-36 VIP peptides Cavia porcellus 161-164 7529994-1 1995 Desensitization of rat pancreatic acinar cells with 0.1 mM carbamoylcholine (Cch) or 0.5 nM caerulein (CAE), a cholecystokinin (CCK) agonist, altered the subsequent secretory responses to these two agonists. Carbachol 59-75 cholecystokinin Rattus norvegicus 111-126 7712026-1 1995 The effect of tumour necrosis factor-alpha (TNF alpha) on the increase in cytosolic free calcium ([Ca2+])i induced by carbachol and bradykinin (BK) was investigated in human tracheal smooth muscle cells in culture (TSMC). Carbachol 118-127 tumor necrosis factor Homo sapiens 44-53 7895929-3 1995 Carbachol (10(-8) to 10(-5) M) dose-dependently stimulated gastrin release with a maximal stimulatory response achieved at a concentration of 10(-5) M (330% over basal). Carbachol 0-9 gastrin Canis lupus familiaris 59-66 7895929-4 1995 To characterize the muscarinic receptor which mediates gastrin release from antral G cells, we examined the effect of three muscarinic receptor antagonists on carbachol-stimulated gastrin release; atropine (nonselective muscarinic receptor antagonist), pirenzepine (M1 muscarinic receptor antagonist) and 4-DAMP (M3 muscarinic receptor antagonist). Carbachol 159-168 gastrin Canis lupus familiaris 180-187 7895929-6 1995 However, carbachol (10(-5) M)-stimulated gastrin release was effectively inhibited by atropine and 4-DAMP with Ki values of 0.48 and 0.66 nM, respectively. Carbachol 9-18 gastrin Canis lupus familiaris 41-48 7895929-7 1995 Pirenzepine at a high concentration (10(-5) M) also inhibited carbachol-stimulated gastrin release with a Ki value of 46.3 nM. Carbachol 62-71 gastrin Canis lupus familiaris 83-90 8587335-1 1995 The effects of endothelin-1 (ET-1) and endothelin-3 (ET-3) were investigated on carbachol-contracted guinea-pig trachea. Carbachol 80-89 endothelin-3 Cavia porcellus 53-57 7475951-6 1995 Biochemical assay of total PKC confirmed that cytosolic enzyme activity was significantly reduced by TPA and carbachol to 29% and 75% respectively of control levels. Carbachol 109-118 protein kinase C, alpha Rattus norvegicus 27-30 7803515-4 1994 Carbamylcholine and PMA caused an increase in membrane-associated alpha PKC, but did not alter the subcellular distribution of zeta PKC. Carbachol 0-15 proline rich transmembrane protein 2 Homo sapiens 72-75 7479339-0 1995 Effect of carbachol on the release of peptide YY from isolated vascularly and luminally perfused rat ileum. Carbachol 10-19 peptide YY Rattus norvegicus 38-48 7479339-4 1995 Carbachol-induced release of PYY into both lumen and vasculature was completely blocked by atropine, but not by hexamethonium. Carbachol 0-9 peptide YY Rattus norvegicus 29-32 7479339-5 1995 Tetrodotoxin abolished carbachol-induced release of PYY into lumen and vasculature. Carbachol 23-32 peptide YY Rattus norvegicus 52-55 7810765-6 1994 As early as 2 min after stop-flow ischemia, the inhibitory effect of carbachol (10 microM) on the stimulation-evoked overflow of norepinephrine and NPY was lost. Carbachol 69-78 pro-neuropeptide Y Cavia porcellus 148-151 7536322-4 1995 Similarly, the effect of EGF on carbachol-stimulated amylase release was substantial at submaximal (0.1 microM: 44% inhibition), maximal (1 microM: 75% inhibition), and supramaximal (100 microM: 33% inhibition) carbachol concentrations. Carbachol 32-41 epidermal growth factor like 1 Rattus norvegicus 25-28 7536322-4 1995 Similarly, the effect of EGF on carbachol-stimulated amylase release was substantial at submaximal (0.1 microM: 44% inhibition), maximal (1 microM: 75% inhibition), and supramaximal (100 microM: 33% inhibition) carbachol concentrations. Carbachol 211-220 epidermal growth factor like 1 Rattus norvegicus 25-28 7536322-6 1995 EGF decreased the peak increase of 1,4,5-IP3 in response to bombesin and carbachol (5 s after beginning of the incubation) and bFGF (15 s after beginning of the incubation) by 81 +/- 19%, 65 +/- 15%, and 56 +/- 18%, respectively. Carbachol 73-82 epidermal growth factor like 1 Rattus norvegicus 0-3 7810765-2 1994 The muscarinic agonists oxotremorine (0.01-1 microM) and carbachol (0.1-10 microM) reduced norepinephrine and NPY overflow in a concentration-dependent manner to approximately 30% of control. Carbachol 57-66 pro-neuropeptide Y Cavia porcellus 110-113 7810765-7 1994 On reperfusion with oxygenated buffer after 10 min of stop-flow ischemia the inhibitory effect of carbachol (10 microM) on stimulation-induced norepinephrine and NPY overflow recovered within 3 min. Carbachol 98-107 pro-neuropeptide Y Cavia porcellus 162-165 7889278-19 1994 These results suggest that there is a significant PKC involvement in constriction of bronchioles to carbachol and 5-HT, and that the proportion of the contractile response that can be attributed to PKC is greater in smaller than larger bronchioles. Carbachol 100-109 protein kinase C, gamma Rattus norvegicus 50-53 7889307-16 1994 Inclusion of SNP (0.01-1 microM) or 8Br-cyclic GMP (50 microM) in the Ca2+-free medium inhibited the transient residual response to carbachol. Carbachol 132-141 5'-nucleotidase, cytosolic II Mus musculus 47-50 7889278-3 1994 In this study we have examined the effects of the specific PKC inhibitors Ro31-8220 and Ro31-7549 and the non-specific inhibitor H7 on carbachol-, 5-hydroxytryptamine (5-HT)- and 4 beta-phorbol dibutyrate (4 beta-PDBu)-induced contractions in large and small bronchioles. Carbachol 135-144 protein kinase C, gamma Rattus norvegicus 59-62 7995178-2 1994 An MLCK inhibitor, 1-(5-chloronaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine (ML-9), significantly inhibited both the basal pepsinogen secretion and the secretion by carbamylcholine chloride (carbachol) or ionomycin without affecting intracellular free Ca2+ concentration ([Ca2+]i), but not by 12-O-tetradecanoylphorbol-13- acetate (TPA) or forskolin. Carbachol 170-194 myosin light chain kinase 3 Homo sapiens 3-7 7995178-2 1994 An MLCK inhibitor, 1-(5-chloronaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine (ML-9), significantly inhibited both the basal pepsinogen secretion and the secretion by carbamylcholine chloride (carbachol) or ionomycin without affecting intracellular free Ca2+ concentration ([Ca2+]i), but not by 12-O-tetradecanoylphorbol-13- acetate (TPA) or forskolin. Carbachol 196-205 myosin light chain kinase 3 Homo sapiens 3-7 7995178-3 1994 A PKC inhibitor, 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7), significantly reduced the pepsinogen secretion by carbachol or TPA, but not by forskolin or ionomycin, and did not affect the basal secretion and the [Ca2+]i elevated by carbachol or ionomycin. Carbachol 120-129 proline rich transmembrane protein 2 Homo sapiens 2-5 7995178-3 1994 A PKC inhibitor, 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7), significantly reduced the pepsinogen secretion by carbachol or TPA, but not by forskolin or ionomycin, and did not affect the basal secretion and the [Ca2+]i elevated by carbachol or ionomycin. Carbachol 240-249 proline rich transmembrane protein 2 Homo sapiens 2-5 7889307-17 1994 Inclusion of similar concentrations of SNP or 8Br-cyclic GMP,during Ca2+ re-loading, increased the subsequent residual contraction to carbachol in Ca2+-free medium.7. Carbachol 134-143 5'-nucleotidase, cytosolic II Mus musculus 57-60 7895910-6 1994 Exposure to the cholinergic agonist carbachol or the protein tyrosine phosphatase inhibitor orthovandate also induced c-fos transcription. Carbachol 36-45 Fos proto-oncogene, AP-1 transcription factor subunit Bos taurus 120-123 7895910-7 1994 Both agents caused repression in c-fos gene activation induced by either IGF-I, carbachol or orthovanadate. Carbachol 80-89 Fos proto-oncogene, AP-1 transcription factor subunit Bos taurus 35-38 7969134-6 1994 Point mutations at conserved residues in the Ras-GAP SH3 domain reversed its action, leading to an increase in carbachol-dependent transformation. Carbachol 111-120 RAS p21 protein activator 1 Mus musculus 45-52 7874280-2 1994 Incubation experiments showed that carbachol (Cch) induced the simultaneous release of glucagon, VIP (vasoactive intestinal polypeptide), and pancreatic polypeptide (PP) at levels significantly higher than basal levels. Carbachol 35-44 vasoactive intestinal peptide Homo sapiens 97-100 7874280-2 1994 Incubation experiments showed that carbachol (Cch) induced the simultaneous release of glucagon, VIP (vasoactive intestinal polypeptide), and pancreatic polypeptide (PP) at levels significantly higher than basal levels. Carbachol 35-44 vasoactive intestinal peptide Homo sapiens 102-135 7874280-2 1994 Incubation experiments showed that carbachol (Cch) induced the simultaneous release of glucagon, VIP (vasoactive intestinal polypeptide), and pancreatic polypeptide (PP) at levels significantly higher than basal levels. Carbachol 35-44 pancreatic polypeptide Homo sapiens 142-164 7874280-2 1994 Incubation experiments showed that carbachol (Cch) induced the simultaneous release of glucagon, VIP (vasoactive intestinal polypeptide), and pancreatic polypeptide (PP) at levels significantly higher than basal levels. Carbachol 35-44 pancreatic polypeptide Homo sapiens 166-168 7874280-2 1994 Incubation experiments showed that carbachol (Cch) induced the simultaneous release of glucagon, VIP (vasoactive intestinal polypeptide), and pancreatic polypeptide (PP) at levels significantly higher than basal levels. Carbachol 46-49 vasoactive intestinal peptide Homo sapiens 97-100 7874280-2 1994 Incubation experiments showed that carbachol (Cch) induced the simultaneous release of glucagon, VIP (vasoactive intestinal polypeptide), and pancreatic polypeptide (PP) at levels significantly higher than basal levels. Carbachol 46-49 vasoactive intestinal peptide Homo sapiens 102-135 7874280-2 1994 Incubation experiments showed that carbachol (Cch) induced the simultaneous release of glucagon, VIP (vasoactive intestinal polypeptide), and pancreatic polypeptide (PP) at levels significantly higher than basal levels. Carbachol 46-49 pancreatic polypeptide Homo sapiens 142-164 7874280-2 1994 Incubation experiments showed that carbachol (Cch) induced the simultaneous release of glucagon, VIP (vasoactive intestinal polypeptide), and pancreatic polypeptide (PP) at levels significantly higher than basal levels. Carbachol 46-49 pancreatic polypeptide Homo sapiens 166-168 7874280-3 1994 Atropine abolished the stimulatory effect of Cch on glucagon, VIP, and PP release. Carbachol 45-48 vasoactive intestinal peptide Homo sapiens 62-65 7969134-7 1994 The inhibitory effect of expression of the Ras-GAP SH3 domain occurs proximal to Ras activation and is selective for the mitogenic pathway activated by carbachol, as cellular transformation by either v-Ras or trkA/nerve growth factor is unaffected. Carbachol 152-161 RAS p21 protein activator 1 Mus musculus 43-50 7947736-9 1994 RHC-80267 inhibits glucose- and carbachol-induced insulin release from intact islets in a dose-dependent manner that parallels its inhibition of diacylglycerol lipase activity. Carbachol 32-41 insulin Homo sapiens 50-57 7977744-2 1994 EGF and TGF-alpha chronically enhanced basal and agonist-stimulated AP accumulation (mean effective concentration 0.6-0.8 nM) but acutely inhibited responses to histamine and carbachol (half-maximal inhibitory concentration approximately 4 nM). Carbachol 175-184 protransforming growth factor alpha Oryctolagus cuniculus 8-17 7825914-0 1994 Modulation of carbachol responsiveness by corticosteroid hormones in rat CA1 pyramidal neurons. Carbachol 14-23 carbonic anhydrase 1 Rattus norvegicus 73-76 7698174-1 1994 In previous studies carbachol-induced stimulation of gastrin release from antral G-cells in primary culture suggested the presence of muscarinic acetylcholine receptors on this cell type. Carbachol 20-29 gastrin Oryctolagus cuniculus 53-60 7698174-6 1994 Maximal gastrin release in response to the acetylcholine receptor agonist carbachol (10(-4) M) or the selective muscarinic receptor agonist arecaidine propargyl ester (10(-4) M) was 8.5 +/- 0.4% and 7.6 +/- 0.4% of total cellular content, respectively. Carbachol 74-83 gastrin Oryctolagus cuniculus 8-15 7524683-3 1994 Treating acini with carbachol abolished the high affinity state of the CCK receptor and converted approximately 25% of the low affinity receptors to the very low affinity state. Carbachol 20-29 cholecystokinin Rattus norvegicus 71-74 7524683-4 1994 Carbachol treatment was particularly useful in establishing the values of Kd for the high and low affinity states for different CCK receptor agonists and antagonists. Carbachol 0-9 cholecystokinin Rattus norvegicus 128-131 7524684-6 1994 Specific receptor antagonists could prevent, as well as reverse completely, the translocation of PKC isoforms caused by CCK-8 or carbachol. Carbachol 129-138 protein kinase C, alpha Rattus norvegicus 97-100 7943332-10 1994 Increased phosphorylation of the MARCKS and other TPA-regulated proteins suggests that CCK, carbachol, bombesin, and the CCK partial agonist, JMV-180, all activate protein kinase C in intact acini. Carbachol 92-101 myristoylated alanine rich protein kinase C substrate Rattus norvegicus 33-39 7854066-5 1994 Treatment of the C2C12 muscle cells with carbachol, a cholinergic agonist, induced zif268 gene expression rapidly and transiently. Carbachol 41-50 early growth response 1 Mus musculus 83-89 7874053-10 1994 We concluded that carbachol and CCK-8 stimulated IF secretion via an increase of intracellular Ca2+ concentration and that secretin did so via a cAMP accumulation. Carbachol 18-27 cobalamin binding intrinsic factor Homo sapiens 49-51 7854066-7 1994 Treatment with ryanodine or dantrolene, which block the calcium release channel of the sarcoplasmic reticulum, inhibited the carbachol-induced zif268 response essentially completely. Carbachol 125-134 early growth response 1 Homo sapiens 143-149 7929563-11 1994 The regulation of bFGF protein content also involves posttranscriptional mechanisms since changes in the levels of individual bFGF isoforms were different depending on whether cells were treated with carbachol or angiotensin II, forskolin, or PMA. Carbachol 200-209 fibroblast growth factor 2 Homo sapiens 18-22 7859812-8 1994 (3) Removal of PLC-beta 1 from the membranes with 2 M KCl resulted in a significant reduction of the CCh-induced PIP2 hydrolysis, and this effect of the muscarinic agonist was restored when the membrane fraction was supplemented with PLC-beta 1 purified from bovine brain. Carbachol 101-104 phospholipase C beta 1 Bos taurus 15-25 7859812-8 1994 (3) Removal of PLC-beta 1 from the membranes with 2 M KCl resulted in a significant reduction of the CCh-induced PIP2 hydrolysis, and this effect of the muscarinic agonist was restored when the membrane fraction was supplemented with PLC-beta 1 purified from bovine brain. Carbachol 101-104 phospholipase C beta 1 Bos taurus 234-244 7929563-11 1994 The regulation of bFGF protein content also involves posttranscriptional mechanisms since changes in the levels of individual bFGF isoforms were different depending on whether cells were treated with carbachol or angiotensin II, forskolin, or PMA. Carbachol 200-209 fibroblast growth factor 2 Homo sapiens 126-130 7823117-4 1994 Bath application of submicromolar concentrations of carbachol (CCh) produced a gradually developing, long-lasting increase in the CA1 excitatory postsynaptic potential and population spike. Carbachol 52-61 carbonic anhydrase 1 Rattus norvegicus 130-133 7948036-2 1994 Binding of [125I]CCK-8 to the high affinity state of the receptor was measured as carbachol-inhibitable binding of [125I]CCK-8, whereas binding of [125I]CCK-8 to the low affinity state was measured as carbachol-resistant binding of [125I]CCK-8. Carbachol 82-91 cholecystokinin Rattus norvegicus 17-20 7823117-4 1994 Bath application of submicromolar concentrations of carbachol (CCh) produced a gradually developing, long-lasting increase in the CA1 excitatory postsynaptic potential and population spike. Carbachol 63-66 carbonic anhydrase 1 Rattus norvegicus 130-133 7948036-2 1994 Binding of [125I]CCK-8 to the high affinity state of the receptor was measured as carbachol-inhibitable binding of [125I]CCK-8, whereas binding of [125I]CCK-8 to the low affinity state was measured as carbachol-resistant binding of [125I]CCK-8. Carbachol 82-91 cholecystokinin Rattus norvegicus 121-124 7948036-2 1994 Binding of [125I]CCK-8 to the high affinity state of the receptor was measured as carbachol-inhibitable binding of [125I]CCK-8, whereas binding of [125I]CCK-8 to the low affinity state was measured as carbachol-resistant binding of [125I]CCK-8. Carbachol 82-91 cholecystokinin Rattus norvegicus 121-124 7948036-2 1994 Binding of [125I]CCK-8 to the high affinity state of the receptor was measured as carbachol-inhibitable binding of [125I]CCK-8, whereas binding of [125I]CCK-8 to the low affinity state was measured as carbachol-resistant binding of [125I]CCK-8. Carbachol 82-91 cholecystokinin Rattus norvegicus 121-124 7948036-2 1994 Binding of [125I]CCK-8 to the high affinity state of the receptor was measured as carbachol-inhibitable binding of [125I]CCK-8, whereas binding of [125I]CCK-8 to the low affinity state was measured as carbachol-resistant binding of [125I]CCK-8. Carbachol 201-210 cholecystokinin Rattus norvegicus 17-20 7854614-0 1994 Altered modulation by excitatory amino acids of cortical phosphatidylinositol system stimulated by carbachol in rats poisoned by an anti-cholinesterase compound, diisopropyl fluorophosphate. Carbachol 99-108 butyrylcholinesterase Rattus norvegicus 137-151 8000501-0 1994 Effects of carbamylcholine chloride on human antral gastrin mRNA levels. Carbachol 11-35 gastrin Homo sapiens 52-59 8000501-2 1994 During a-2-h incubation period, carbachol (10(-6)-10(-4) M) decreased gastrin mRNA levels to 71 +/- 8% (10(-6) M), 40 +/- 8% (10(-5) M), and 33 +/- 5% (10(-4) M) of control levels. Carbachol 32-41 gastrin Homo sapiens 70-77 8000501-3 1994 Carbachol (10(-5) M) decreased intracellular gastrin (from 1634 +/- 103 to 1272 +/- 126 pg/mg tissue protein), while it increased gastrin release into the medium (from 609 +/- 48 to 918 +/- 68 pg/ml per mg tissue protein). Carbachol 0-9 gastrin Homo sapiens 45-52 8000501-3 1994 Carbachol (10(-5) M) decreased intracellular gastrin (from 1634 +/- 103 to 1272 +/- 126 pg/mg tissue protein), while it increased gastrin release into the medium (from 609 +/- 48 to 918 +/- 68 pg/ml per mg tissue protein). Carbachol 0-9 gastrin Homo sapiens 130-137 8000501-4 1994 After 6- and 9-h culture, carbachol gradually increased gastrin mRNA levels, by 96 +/- 12% and 126 +/- 23%, respectively. Carbachol 26-35 gastrin Homo sapiens 56-63 8000501-7 1994 These findings suggested that carbachol may have a time-related biphasic action on human antral gastrin biosynthesis. Carbachol 30-39 gastrin Homo sapiens 96-103 8092325-0 1994 Comparison of fos-like immunoreactivity induced in rat brain by central injection of angiotensin II and carbachol. Carbachol 104-113 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 14-17 7813590-7 1994 In the guinea pig ileum stimulated by carbachol, endothelin-3 induced a transient relaxation followed by sustained relaxation. Carbachol 38-47 endothelin-3 Cavia porcellus 49-61 8092325-8 1994 The AT1 antagonist prevented carbachol-induced FLI in the MnPO only. Carbachol 29-38 angiotensin II receptor, type 1a Rattus norvegicus 4-7 7841454-5 1994 Mepyramine, a histamine H1-receptor antagonist, moderately diminished the carbachol-induced corticosterone response and abolished the rise in hypothalamic histamine levels. Carbachol 74-83 histamine receptor H 1 Rattus norvegicus 14-35 7812636-7 1994 Repeated periods of illumination at 1.1 x 10(4) lux produced a reversible relaxation of both carbachol and KCl-evoked tone in muscle pretreated with RBS (50 microM). Carbachol 93-102 establishment of sister chromatid cohesion N-acetyltransferase 2 Homo sapiens 149-152 7824043-11 1994 We conclude that prolonged incubation with carbachol in rat neonatal ventricular myocytes causes a reduction in cell surface beta 1-Adrenoceptor density. Carbachol 43-52 adrenoceptor beta 1 Rattus norvegicus 125-144 7935319-6 1994 In addition, cells pretreated with carbachol or thrombin show a normal response to phorbol-12-myristate-13-acetate, suggesting that the enzymatic activity of PLD is not compromised. Carbachol 35-44 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 158-161 7935319-9 1994 Thrombin and carbachol cause comparable changes in redistribution of both PKC-alpha and PKC-epsilon. Carbachol 13-22 protein kinase C alpha Homo sapiens 74-83 7935319-9 1994 Thrombin and carbachol cause comparable changes in redistribution of both PKC-alpha and PKC-epsilon. Carbachol 13-22 protein kinase C epsilon Homo sapiens 88-99 7830883-5 1994 Stimulation of the chromaffin cells with the cholinergic agonist carbachol (10(-4) M) increased in parallel the output of neurotrophic factor activity (assayed on chick ciliary ganglionic neurons) as well as two components specifically located in chromaffin granules, chromogranin A and catecholamines. Carbachol 65-74 chromogranin A Gallus gallus 268-282 8076696-1 1994 The purpose of this study was to determine the role of protein kinase C (PKC) isozymes in carbachol-induced protein secretion in the lacrimal gland. Carbachol 90-99 protein kinase C, alpha Rattus norvegicus 73-76 8063729-5 1994 Carbachol potently induced c-Raf activity as judged by its in vitro phosphorylating activity using MEK as a substrate. Carbachol 0-9 v-raf-leukemia viral oncogene 1 Mus musculus 27-32 8063729-5 1994 Carbachol potently induced c-Raf activity as judged by its in vitro phosphorylating activity using MEK as a substrate. Carbachol 0-9 midkine Mus musculus 99-102 8063729-6 1994 However, induction of Raf-1 kinase activity by carbachol occurred much earlier than changes in its electrophoretic mobility. Carbachol 47-56 v-raf-leukemia viral oncogene 1 Mus musculus 22-27 8063729-9 1994 However, c-Raf and ERK2 enzymatic activity in response to carbachol was at least 50-80% PKC-independent. Carbachol 58-67 v-raf-leukemia viral oncogene 1 Mus musculus 9-14 8063729-9 1994 However, c-Raf and ERK2 enzymatic activity in response to carbachol was at least 50-80% PKC-independent. Carbachol 58-67 mitogen-activated protein kinase 1 Mus musculus 19-23 7945415-5 1994 The PDE IV inhibitors BPX and Ro 20-1724 synergistically increased the relaxant effect of the beta 2-adrenoceptor agonist salbutamol in carbachol-contracted trachea much more strongly than theophylline. Carbachol 136-145 beta-2 adrenergic receptor Cavia porcellus 94-113 7800852-2 1994 Carbachol (Cch) stimulated the secretion of CGA and PST from QGP-1N cells derived from a human pancreatic islet cell tumor. Carbachol 0-9 chromogranin A Homo sapiens 44-47 7916119-2 1994 In normal dogs, ICV carbachol stimulated marked counterregulatory hormone release, but altered plasma glucose only marginally because the marked increment in glucose production (Ra) was almost matched by the increment of utilization (Rd), even though plasma insulin was unchanged. Carbachol 20-29 insulin Canis lupus familiaris 258-265 7800852-0 1994 Production and secretion of chromogranin A and pancreastatin by the human pancreatic carcinoma cell line QGP-1N on stimulation with carbachol. Carbachol 132-141 chromogranin A Homo sapiens 28-42 7800852-2 1994 Carbachol (Cch) stimulated the secretion of CGA and PST from QGP-1N cells derived from a human pancreatic islet cell tumor. Carbachol 11-14 chromogranin A Homo sapiens 44-47 7800852-6 1994 Stimulation with Cch increased the total amount of PST in the cells and the medium 1.7-fold and decreased the amount of CGA in the cells and medium. Carbachol 17-20 chromogranin A Homo sapiens 120-123 7800852-8 1994 Stimulation with Cch resulted in an increase in the intensity of PST-immunoreactive bands and a decrease in those of CGA-immunoreactive bands. Carbachol 17-20 chromogranin A Homo sapiens 117-120 7800852-10 1994 These results suggested that (1) the secretion of CGA and PST from QGP-1N cells is regulated mainly through muscarinic receptors coupled with activation of polyphosphoinositide breakdown by a G protein, with intracellular calcium ion and protein kinase C playing a role in the stimulus-secretion coupling and that (2) Cch may induce the secretion of PST and CGA and processing from CGA to PST. Carbachol 318-321 chromogranin A Homo sapiens 50-53 7520939-7 1994 IL-1 alpha (500 ng/ml) also inhibited carbachol-stimulated AP accumulation. Carbachol 38-47 interleukin 1 alpha Canis lupus familiaris 0-10 7520939-9 1994 TNF-alpha also significantly inhibited histamine/IMX- and carbachol-stimulated AP uptake (P < or = .01). Carbachol 58-67 tumor necrosis factor Canis lupus familiaris 0-9 8046453-4 1994 In the present experiments we use extracellular recording and immunocytochemistry for Fos, the protein product of c-fos, to demonstrate the activation of the cells following intracerebroventricular administration of the muscarinic agonist, carbachol. Carbachol 240-249 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 86-89 7520939-11 1994 These results show that IL-1 alpha and TNF-alpha inhibit histamine- and carbachol-stimulated isolated parietal cell secretion and that, for IL-1 alpha, this effect does not depend on mucosal prostaglandin synthesis. Carbachol 72-81 interleukin 1 alpha Canis lupus familiaris 24-34 7520939-11 1994 These results show that IL-1 alpha and TNF-alpha inhibit histamine- and carbachol-stimulated isolated parietal cell secretion and that, for IL-1 alpha, this effect does not depend on mucosal prostaglandin synthesis. Carbachol 72-81 tumor necrosis factor Canis lupus familiaris 39-48 8046453-4 1994 In the present experiments we use extracellular recording and immunocytochemistry for Fos, the protein product of c-fos, to demonstrate the activation of the cells following intracerebroventricular administration of the muscarinic agonist, carbachol. Carbachol 240-249 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 114-119 8046453-5 1994 Fos expression following carbachol injection was then compared with that induced by a similar number of antidromically evoked action potentials. Carbachol 25-34 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 0-3 8026585-3 1994 The concentration-response curve of carbachol for adrenomedullin secretion (EC50 42 microM) was similar to that for catecholamine secretion (EC50 63 microM). Carbachol 36-45 adrenomedullin Bos taurus 50-64 7519402-8 1994 Thus GP-2 secretion appears to be modulated by two discrete cellular processes: 1) delivery of prereleased GP-2 within zymogen granules to the ductal lumen by exocytic mechanisms and 2) enzymatic release of GPI-anchored GP-2 from the luminal membranes, a kinetic process that appears to be regulated by secretin- or carbachol-induced secretion of bicarbonate. Carbachol 316-325 glycoprotein 2 Rattus norvegicus 5-9 7921598-22 1994 The maximal response to ET-1 was less than 20% of that to the cholinoceptor agonist, carbachol. Carbachol 85-94 endothelin 1 Homo sapiens 24-28 8207426-4 1994 Carbachol-stimulated phosphoinositidase C activity in permeabilized SH-SY5Y cells was also shown to be highly dependent on free Ca2+ concentration (20-100 nM) and suggests that under normal conditions, InsP3 formation is enhanced by increases in cytosolic free Ca2+ concentration that accompany muscarinic receptor activation. Carbachol 0-9 phospholipase C beta 1 Homo sapiens 21-41 8207426-4 1994 Carbachol-stimulated phosphoinositidase C activity in permeabilized SH-SY5Y cells was also shown to be highly dependent on free Ca2+ concentration (20-100 nM) and suggests that under normal conditions, InsP3 formation is enhanced by increases in cytosolic free Ca2+ concentration that accompany muscarinic receptor activation. Carbachol 0-9 carbonic anhydrase 2 Homo sapiens 128-131 8207426-4 1994 Carbachol-stimulated phosphoinositidase C activity in permeabilized SH-SY5Y cells was also shown to be highly dependent on free Ca2+ concentration (20-100 nM) and suggests that under normal conditions, InsP3 formation is enhanced by increases in cytosolic free Ca2+ concentration that accompany muscarinic receptor activation. Carbachol 0-9 carbonic anhydrase 2 Homo sapiens 261-264 7516704-1 1994 The secondary structure and effects of two ligands, carbamylcholine and tetracaine, on the secondary structure of affinity-purified nicotinic acetylcholine receptor (nAChR) from Torpedo has been studied using Fourier transform infrared spectroscopy (FTIR). Carbachol 52-67 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 132-164 7964998-0 1994 Carbachol-induced synchronized rhythmic bursts in CA3 neurons of guinea pig hippocampus in vitro. Carbachol 0-9 carbonic anhydrase 3 Cavia porcellus 50-53 7964998-2 1994 Carbachol effects on CA3 hippocampal cells were studied in the absence of ionotropic glutamatergic and GABAergic transmission with intracellular and extracellular recordings from guinea pig septohippocampal slices. Carbachol 0-9 carbonic anhydrase 3 Cavia porcellus 21-24 7516704-1 1994 The secondary structure and effects of two ligands, carbamylcholine and tetracaine, on the secondary structure of affinity-purified nicotinic acetylcholine receptor (nAChR) from Torpedo has been studied using Fourier transform infrared spectroscopy (FTIR). Carbachol 52-67 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 166-171 7925609-1 1994 In this study, the signal cascade transducing carbachol stimulation into c-fos expression in SH-SY5Y neuroblastoma cells was investigated. Carbachol 46-55 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 73-78 7925609-3 1994 Application of 1,2-dioctanoylglycerol induced c-fos expression and this, as well as carbachol-stimulated c-fos expression, was inhibited by protein kinase C inhibitors. Carbachol 84-93 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 105-110 7925609-6 1994 The carbachol-stimulated c-fos expression was potentiated by application of the protein phosphatase inhibitor okadaic acid. Carbachol 4-13 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 25-30 7525030-13 1994 This was attributed to an inhibitory G protein (Gi) mechanism, as the muscarinic agonist carbachol, which acts through Gi, produced the same effects as AII. Carbachol 89-98 angiotensinogen Rattus norvegicus 152-155 8207320-6 1994 The contractile effect of 100 microM carbachol was antagonized by pretreatment of atropine (1 microM) and 4DAMP (10 nM, antagonist selective for the M1 and M3 receptors) but not by pirenzepine (10 microM, antagonist selective for the M1 receptor), suggesting the involvement of the M3 but not the M1 muscarinic receptor. Carbachol 37-46 cholinergic receptor muscarinic 1 Homo sapiens 149-168 7523819-2 1994 TRH showed a dose-dependent inhibition of carbachol-stimulated amylase secretion with a maximum of 24% at a concentration of 10(-11) M (p < 0.05). Carbachol 42-51 thyrotropin releasing hormone Rattus norvegicus 0-3 7912276-8 1994 High extracellular potassium concentrations or carbachol evoked release of endogenous VIP. Carbachol 47-56 VIP peptides Cavia porcellus 86-89 7936115-8 1994 In the presence of the vasopressin antagonist, d(CH2)5(Tyr(Et))DAVP, alone or in combination with phentolamine and propranolol, the pressor response to carbachol was substantially reduced, while the renal and superior mesenteric vasoconstrictor effects were completely abolished; the bradycardia was not significantly affected by this treatment. Carbachol 152-161 arginine vasopressin Rattus norvegicus 23-34 7936115-9 1994 These results indicate an important involvement of vasopressin in the cardiovascular responses to carbachol injected into the PVN of untreated animals. Carbachol 98-107 arginine vasopressin Rattus norvegicus 51-62 7936115-10 1994 Moreover, in the presence of the vasopressin antagonist the hindquarters vascular bed showed a vasodilatation following PVN injection of carbachol; this effect was reversed to a vasoconstriction following combined i.v. Carbachol 137-146 arginine vasopressin Rattus norvegicus 33-44 8203594-5 1994 Carbachol increased 35S-labeled guanosine 5"-O-(3-thiotriphosphate) (GTP gamma S) binding maximally 14-fold only when GDP was present by an excess of > 100 times [35S]GTP gamma S, while R-PIA increased binding only 2-fold. Carbachol 0-9 RPTOR independent companion of MTOR complex 2 Homo sapiens 191-194 8156901-6 1994 The cholinergic agonist carbachol stimulated GLP-1 secretion in a concentration-dependent manner, maximal release was observed at 1 mM carbachol (228% of the control value). Carbachol 24-33 glucagon Mus musculus 45-50 8156901-6 1994 The cholinergic agonist carbachol stimulated GLP-1 secretion in a concentration-dependent manner, maximal release was observed at 1 mM carbachol (228% of the control value). Carbachol 135-144 glucagon Mus musculus 45-50 8182471-1 1994 We have investigated the regulation of phosphorylation of the nicotinic ACh receptor (nAChR) in rat myotubes by the agonist carbamylcholine. Carbachol 124-139 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 62-84 8182471-1 1994 We have investigated the regulation of phosphorylation of the nicotinic ACh receptor (nAChR) in rat myotubes by the agonist carbamylcholine. Carbachol 124-139 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 86-91 8182471-4 1994 Pretreatment of myotubes with d-tubocurare, but not with atropine, inhibited carbamylcholine-stimulated phosphorylation of the nAChR. Carbachol 77-92 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 127-132 8182471-5 1994 Preincubation with open-channel blockers of the nAChR also inhibited phosphorylation of the nAChR induced by carbamylcholine. Carbachol 109-124 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 48-53 8182471-5 1994 Preincubation with open-channel blockers of the nAChR also inhibited phosphorylation of the nAChR induced by carbamylcholine. Carbachol 109-124 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 92-97 8182471-6 1994 Depletion of extracellular calcium from myotube cultures prevented carbamylcholine-stimulated increases in nAChR phosphorylation whereas application of a calcium ionophore mimicked the effect of carbamylcholine on nAChR phosphorylation. Carbachol 67-82 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 107-112 8182471-6 1994 Depletion of extracellular calcium from myotube cultures prevented carbamylcholine-stimulated increases in nAChR phosphorylation whereas application of a calcium ionophore mimicked the effect of carbamylcholine on nAChR phosphorylation. Carbachol 67-82 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 214-219 8182471-6 1994 Depletion of extracellular calcium from myotube cultures prevented carbamylcholine-stimulated increases in nAChR phosphorylation whereas application of a calcium ionophore mimicked the effect of carbamylcholine on nAChR phosphorylation. Carbachol 195-210 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 214-219 8182471-8 1994 Carbamylcholine increased nAChR phosphorylation to the same extent and with the same time course and subunit specificity as that induced by phorbol esters. Carbachol 0-15 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 26-31 7916769-1 1994 Acute pretreatment (30 min) of primary cultures of cerebellar granule cells with TPA (10 nM) resulted in a decrease in carbachol-and glutamate-stimulated phosphoinositide hydrolysis, but not in basal levels of PI hydrolysis. Carbachol 119-128 plasminogen activator, tissue type Homo sapiens 81-84 7916769-5 1994 TPA treatment results in a statistically significant decrease in glutamate-stimulated PI hydrolysis, and a slight reduction of carbachol-stimulated PI hydrolysis when compared to temporally matched controls. Carbachol 127-136 plasminogen activator, tissue type Homo sapiens 0-3 8197564-8 1994 Incubation experiments revealed a simultaneous in vitro release of VIP and PP with a significant increase by either carbachol or phorbol myristate acetate but not by theophylline or caerulein. Carbachol 116-125 vasoactive intestinal peptide Homo sapiens 67-70 8197564-8 1994 Incubation experiments revealed a simultaneous in vitro release of VIP and PP with a significant increase by either carbachol or phorbol myristate acetate but not by theophylline or caerulein. Carbachol 116-125 pancreatic polypeptide Homo sapiens 75-77 8197564-9 1994 Atropine completely abolished the stimulatory effects of carbachol on VIP and PP release. Carbachol 57-66 vasoactive intestinal peptide Homo sapiens 70-73 8197564-9 1994 Atropine completely abolished the stimulatory effects of carbachol on VIP and PP release. Carbachol 57-66 pancreatic polypeptide Homo sapiens 78-80 8203594-3 1994 Addition of (-)-N6-(2-phenylisopropyl)adenosine (R-PIA) in the presence of carbachol did not further reduce force of contraction. Carbachol 75-84 RPTOR independent companion of MTOR complex 2 Homo sapiens 51-54 8182531-10 1994 Taken together, these results suggest that the coupling of m1 receptors to a putative phospholipase A2 that is positively regulated by protein kinase C and by calcium is necessary for the carbachol-evoked release of AA. Carbachol 188-197 phospholipase A2, group IB, pancreas Mus musculus 86-102 7913969-9 1994 Effects of the NO-releasing agent molsidomine (SIN-1) on CCh-induced changes in ICa(L) were also investigated. Carbachol 57-60 MAPK associated protein 1 Homo sapiens 47-52 8137762-5 1994 PTHrP mRNA expression was weak in the physiological fasting and feeding states, but was markedly increased by pyloric ligation and carbachol stimulation. Carbachol 131-140 parathyroid hormone-like hormone Rattus norvegicus 0-5 8045358-7 1994 However, carbachol treatment did elicit STC release as revealed by the lowering of JinCa2+. Carbachol 9-18 stanniocalcin Oncorhynchus mykiss 40-43 7515904-12 1994 This effect was prevented by N(G)-nitro-L-arginine (L-NNA), which also inhibited VIP-stimulated secretion of amylase; however, L-NNA had no effect on amylase secretion stimulated by carbachol. Carbachol 182-191 vasoactive intestinal peptide Homo sapiens 81-84 7512633-3 1994 Carbachol, which activates both nicotinic and muscarinic receptors, produces 12-19-fold increases in PNMT mRNA and a 22-fold increase in epinephrine release. Carbachol 0-9 phenylethanolamine N-methyltransferase Bos taurus 101-105 7512633-4 1994 Selective nicotinic and muscarinic antagonists (hexamethonium and atropine) each partially reduce carbachol-stimulated increases in PNMT mRNA while a combination of both eliminates > 90% of the carbachol response, thus indicating that separable nicotinic and muscarinic components contribute to the cholinergic increase in PNMT mRNA. Carbachol 98-107 phenylethanolamine N-methyltransferase Bos taurus 132-136 7512633-4 1994 Selective nicotinic and muscarinic antagonists (hexamethonium and atropine) each partially reduce carbachol-stimulated increases in PNMT mRNA while a combination of both eliminates > 90% of the carbachol response, thus indicating that separable nicotinic and muscarinic components contribute to the cholinergic increase in PNMT mRNA. Carbachol 98-107 phenylethanolamine N-methyltransferase Bos taurus 326-330 7513126-3 1994 Carbachol also dose-dependently increased HDC activity (EC50 7.08 +/- 0.32 nM), and its effect was reversed by selective muscarinic-receptor antagonists with the following order of potency: pirenzepine (IC50 15.1 +/- 1.2 nM) > para-fluoro-hexahydro-siladifenidol (IC50 0.316 +/- 0.02 microM) > 11-2[(2-[(diethyl-amino)-methyl]-1-piperidinyl)acetyl]-5,11-dihydro-6H- pyrido[2,3-b][1,4]benzodiazepine-6-one (IC50 28.5 +/- 1.1 microM). Carbachol 0-9 histidine decarboxylase Oryctolagus cuniculus 42-45 7517089-4 1994 Different from CCK-8, EGF reduced amylase release at both submaximal (< or = 1 microM) and supramaximal (> 1 microM) carbachol concentrations. Carbachol 123-132 epidermal growth factor like 1 Rattus norvegicus 22-25 7517089-5 1994 Moreover, EGF (17 nM) inhibited bombesin-, carbachol-, and CCK-8-induced 1,4,5-IP3-production at five seconds after beginning of the incubation by 81 +/- 19%, 65 +/- 15%, and 60 +/- 12%, respectively. Carbachol 43-52 epidermal growth factor like 1 Rattus norvegicus 10-13 7912604-5 1994 The inhibitory effect of somatostatin on secretin-induced pepsinogen secretion was abolished by pretreatment with pertussis toxin, but inhibition of forskolin-, carbachol- and cholecystokinin octapeptide-induced pepsinogen secretion was not. Carbachol 161-170 somatostatin Homo sapiens 25-37 7509377-8 1994 Secretory activity was elevated by both forms of c-src in response to either nicotine or carbachol (which activate the nicotinic and the nicotinic/muscarinic receptors, respectively). Carbachol 89-98 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 49-54 8172620-0 1994 Role of calcium in carbachol- and neurotensin-induced mucin exocytosis in a human colonic goblet cell line and cross-talk with the cyclic AMP pathway. Carbachol 19-28 LOC100508689 Homo sapiens 54-59 8172620-2 1994 Carbachol, a cholinergic agonist, induced an initial concentration-dependent [Ca2+]i peak increasing from 129 +/- 3 nM (basal [Ca2+]i) to 608 +/- 101 nM at 1 x 10(-4) M carbachol with an ED50 of 7 microM, and this was followed by a lower-level plateau. Carbachol 0-9 carbonic anhydrase 2 Homo sapiens 78-81 8172620-2 1994 Carbachol, a cholinergic agonist, induced an initial concentration-dependent [Ca2+]i peak increasing from 129 +/- 3 nM (basal [Ca2+]i) to 608 +/- 101 nM at 1 x 10(-4) M carbachol with an ED50 of 7 microM, and this was followed by a lower-level plateau. Carbachol 0-9 carbonic anhydrase 2 Homo sapiens 127-130 8172620-2 1994 Carbachol, a cholinergic agonist, induced an initial concentration-dependent [Ca2+]i peak increasing from 129 +/- 3 nM (basal [Ca2+]i) to 608 +/- 101 nM at 1 x 10(-4) M carbachol with an ED50 of 7 microM, and this was followed by a lower-level plateau. Carbachol 169-178 carbonic anhydrase 2 Homo sapiens 78-81 8172620-8 1994 Carbachol-induced mucin exocytosis was concentration-dependent with an ED50 of 15 microM, and was inhibited by 35% upon removal of extracellular Ca2+. Carbachol 0-9 LOC100508689 Homo sapiens 18-23 8172620-8 1994 Carbachol-induced mucin exocytosis was concentration-dependent with an ED50 of 15 microM, and was inhibited by 35% upon removal of extracellular Ca2+. Carbachol 0-9 carbonic anhydrase 2 Homo sapiens 145-148 8172620-10 1994 Together our results suggest that while the mucin secretory response to carbachol depends on both the release of Ca2+ from intracellular stores and a Ca2+ influx from external medium, the secretory response to neurotensin is based solely on intracellular Ca2+ mobilization. Carbachol 72-81 LOC100508689 Homo sapiens 44-49 8172620-10 1994 Together our results suggest that while the mucin secretory response to carbachol depends on both the release of Ca2+ from intracellular stores and a Ca2+ influx from external medium, the secretory response to neurotensin is based solely on intracellular Ca2+ mobilization. Carbachol 72-81 carbonic anhydrase 2 Homo sapiens 113-116 8172620-10 1994 Together our results suggest that while the mucin secretory response to carbachol depends on both the release of Ca2+ from intracellular stores and a Ca2+ influx from external medium, the secretory response to neurotensin is based solely on intracellular Ca2+ mobilization. Carbachol 72-81 carbonic anhydrase 2 Homo sapiens 150-153 8172620-10 1994 Together our results suggest that while the mucin secretory response to carbachol depends on both the release of Ca2+ from intracellular stores and a Ca2+ influx from external medium, the secretory response to neurotensin is based solely on intracellular Ca2+ mobilization. Carbachol 72-81 carbonic anhydrase 2 Homo sapiens 150-153 8172620-11 1994 Finally, evaluation of the cross-talk between the cyclic AMP pathway stimulated by vasoactive intestinal peptide (VIP) and the Ca2+ pathway stimulated by neurotensin or carbachol led to the conclusion that the potentiated secretory response elicited by the combined action of carbachol and VIP requires extracellular Ca2+. Carbachol 169-178 carbonic anhydrase 2 Homo sapiens 127-130 8141291-3 1994 Enprostil (10(-10)-10(-6) M) inhibited gastrin secretion in response to bombesin, carbachol, and forskolin, the latter a receptor-independent activator of adenylate cyclase. Carbachol 82-91 gastrin Canis lupus familiaris 39-46 8004403-19 1994 The response to carbachol appeared to be mediated by an M3-muscarinic receptor (apparent Kd for pirenzepine 0.09 microM). Carbachol 16-25 cholinergic receptor muscarinic 3 Homo sapiens 56-78 8175604-4 1994 Airway responsiveness was expressed as the cumulative provocative dose of carbachol that increased sRL to 4 cmH2O/s [PD4, in breath units (BU; 1 BU = 1 breath of 1% carbachol solution)]. Carbachol 74-83 sarcalumenin Rattus norvegicus 99-102 7509388-4 1994 Carbachol increased cyclic GMP formation in the mixture of cells in a time- and concentration-dependent manner, with a half-maximal response occurring in the nanomolar range. Carbachol 0-9 5'-nucleotidase, cytosolic II Mus musculus 27-30 7513126-4 1994 Moreover, gastrin and carbachol were able to modify slightly but significantly both the Michaelis constant (Km) and the maximal velocity (Vmax) of HDC in the same way (18-20% reduction of the Km and 25-30% increase of the Vmax). Carbachol 22-31 histidine decarboxylase Oryctolagus cuniculus 147-150 8297348-9 1994 The specific PKC inhibitor RO 31-7459 (10 microM) was found to inhibit K(+)- and carbachol-evoked release by 27% and 68% respectively. Carbachol 81-90 protein kinase C alpha Homo sapiens 13-16 7509439-7 1994 The increase in substance P binding induced by carbamylcholine was blocked by the nicotinic antagonists alpha-bungarotoxin and d-tubocurarine but was not affected by the muscarinic antagonist atropine. Carbachol 47-62 tachykinin precursor 1 Homo sapiens 16-27 7907526-3 1994 Here, we report that the alpha 2 antagonist idazoxan (IZ) enhances REM-like sleep states in the cat when microinfused into the rostral part of the mPRF at sites where carbachol produced a marked increase in REM. Carbachol 167-176 Spi-C transcription factor (Spi-1/PU.1 related) Mus musculus 147-151 8297348-11 1994 These data suggest that PKC-alpha and/or PKC-epsilon play an essential role in the regulation of PMA-enhanced K(+)- and carbachol-evoked NA release in SH-SY5Y cells. Carbachol 120-129 protein kinase C alpha Homo sapiens 24-33 8297348-11 1994 These data suggest that PKC-alpha and/or PKC-epsilon play an essential role in the regulation of PMA-enhanced K(+)- and carbachol-evoked NA release in SH-SY5Y cells. Carbachol 120-129 protein kinase C epsilon Homo sapiens 41-52 7508198-5 1994 Preincubation of carbachol-treated muscle strips with calphostin C restored the ability of VIP to stimulate L-[3H]citrulline production and the ability of L-NNA to inhibit VIP-induced relaxation. Carbachol 17-26 VIP peptides Oryctolagus cuniculus 91-94 7508198-5 1994 Preincubation of carbachol-treated muscle strips with calphostin C restored the ability of VIP to stimulate L-[3H]citrulline production and the ability of L-NNA to inhibit VIP-induced relaxation. Carbachol 17-26 VIP peptides Oryctolagus cuniculus 172-175 7914493-10 1994 However, acetylcholinesterase does not hydrolyse carbachol and therefore it is necessary to postulate a different post-synaptic mechanism to explain the lesion-induced increases in the sensitivities of individual neurones to carbachol and to acetylcholine; interpretation of experimental findings should take these two mechanisms into account. Carbachol 225-234 acetylcholinesterase Rattus norvegicus 9-29 8201782-5 1994 Activation of the KCa channel was suppressed when the intracellular production of cAMP was suppressed by preincubation with carbachol (10(-6) M). Carbachol 124-133 casein kappa Homo sapiens 18-21 8014894-12 1994 Treatment (24-48 h) of VMH neurones with antisense phosphothio-oligodeoxynucleotides to the G alpha 11 G-protein subunit (10 microM) significantly diminished the carbachol-induced depression of IK. Carbachol 162-171 G protein subunit alpha 11 Rattus norvegicus 92-102 8134614-2 1993 Carbachol stimulated secretion of neurotensin concentration-dependently in the range of 10(-6) - 10(-4) M. The neurotensin secretion stimulated with 10(-5) M carbachol was completely inhibited by atropine at 10(-5) M. Phorbol ester and calcium ionophore (A23187) stimulated secretion of neurotensin. Carbachol 0-9 neurotensin Homo sapiens 34-45 7997057-10 1994 Interestingly, neuropeptides such as neurotensin, cholecystokinin and VIP can potentiate carbachol-stimulated phosphoinositide hydrolysis in frontal cortex of both young and aged rats. Carbachol 89-98 vasoactive intestinal peptide Rattus norvegicus 70-73 18475595-3 1994 Furthermore, interferon gamma increased the affinity of carbachol for the cholinoceptors and did not change its maximum effect. Carbachol 56-65 interferon gamma Rattus norvegicus 13-29 7535425-7 1994 VIP and galanin alone had no effect on cell length, but each caused a dose-dependent inhibition of carbachol-induced contraction and both had an EC50 of 3-7 nM. Carbachol 99-108 VIP peptides Cavia porcellus 0-3 8262183-4 1993 Phosphorylation of the m3-muscarinic receptor was agonist dependent, reaching a maximum after 10 min exposure to carbachol (1 mM) and was completely blocked by atropine (10 microM). Carbachol 113-122 cholinergic receptor muscarinic 3 Homo sapiens 23-45 8974323-5 1994 Stimulation with CCh also induced expression of the immediate-early gene c-fos in these cells. Carbachol 17-20 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 73-78 7816994-7 1994 The authors report evidence of non-specific bronchial hyper-reactivity (HRB) to Carbachol in a patient effected with benign cutaneous mastocytosis with secondary elevation of the total serum IGE. Carbachol 80-89 ArfGAP with FG repeats 1 Homo sapiens 72-75 8134614-2 1993 Carbachol stimulated secretion of neurotensin concentration-dependently in the range of 10(-6) - 10(-4) M. The neurotensin secretion stimulated with 10(-5) M carbachol was completely inhibited by atropine at 10(-5) M. Phorbol ester and calcium ionophore (A23187) stimulated secretion of neurotensin. Carbachol 0-9 neurotensin Homo sapiens 111-122 8134614-2 1993 Carbachol stimulated secretion of neurotensin concentration-dependently in the range of 10(-6) - 10(-4) M. The neurotensin secretion stimulated with 10(-5) M carbachol was completely inhibited by atropine at 10(-5) M. Phorbol ester and calcium ionophore (A23187) stimulated secretion of neurotensin. Carbachol 0-9 neurotensin Homo sapiens 111-122 8134614-2 1993 Carbachol stimulated secretion of neurotensin concentration-dependently in the range of 10(-6) - 10(-4) M. The neurotensin secretion stimulated with 10(-5) M carbachol was completely inhibited by atropine at 10(-5) M. Phorbol ester and calcium ionophore (A23187) stimulated secretion of neurotensin. Carbachol 158-167 neurotensin Homo sapiens 34-45 8134614-2 1993 Carbachol stimulated secretion of neurotensin concentration-dependently in the range of 10(-6) - 10(-4) M. The neurotensin secretion stimulated with 10(-5) M carbachol was completely inhibited by atropine at 10(-5) M. Phorbol ester and calcium ionophore (A23187) stimulated secretion of neurotensin. Carbachol 158-167 neurotensin Homo sapiens 111-122 8134614-2 1993 Carbachol stimulated secretion of neurotensin concentration-dependently in the range of 10(-6) - 10(-4) M. The neurotensin secretion stimulated with 10(-5) M carbachol was completely inhibited by atropine at 10(-5) M. Phorbol ester and calcium ionophore (A23187) stimulated secretion of neurotensin. Carbachol 158-167 neurotensin Homo sapiens 111-122 8134614-5 1993 Neurotensin released into culture medium through stimulation with carbachol coeluted with neurotensin 1-13 on a gel-chromatography. Carbachol 66-75 neurotensin Homo sapiens 0-11 8134614-5 1993 Neurotensin released into culture medium through stimulation with carbachol coeluted with neurotensin 1-13 on a gel-chromatography. Carbachol 66-75 neurotensin Homo sapiens 90-101 8250938-1 1993 The phospholipase A2 (PLA2) inhibitors quinacrine, manoalide and scalaradial inhibit the carbachol-stimulated secretion of the amyloid precursor protein (APP) from cells transfected with the human m1 muscarinic receptor. Carbachol 89-98 phospholipase A2 group IB Homo sapiens 4-20 8243834-7 1993 However, anti-G alpha q completely inhibited the Ca(2+)-mobilizing effect of carbachol, but not of glucose, within 5 min. Carbachol 77-86 guanine nucleotide binding protein, alpha q polypeptide Mus musculus 14-23 8250938-1 1993 The phospholipase A2 (PLA2) inhibitors quinacrine, manoalide and scalaradial inhibit the carbachol-stimulated secretion of the amyloid precursor protein (APP) from cells transfected with the human m1 muscarinic receptor. Carbachol 89-98 phospholipase A2 group IB Homo sapiens 22-26 8250938-1 1993 The phospholipase A2 (PLA2) inhibitors quinacrine, manoalide and scalaradial inhibit the carbachol-stimulated secretion of the amyloid precursor protein (APP) from cells transfected with the human m1 muscarinic receptor. Carbachol 89-98 amyloid beta precursor protein Homo sapiens 127-152 7510053-1 1993 Experiments were performed to investigate the mechanism underlying the potentiation of N-methyl-D-aspartate (NMDA) responses by carbachol (CCh) in the CA1 region of rat hippocampal slices. Carbachol 128-137 carbonic anhydrase 1 Rattus norvegicus 151-154 7510053-1 1993 Experiments were performed to investigate the mechanism underlying the potentiation of N-methyl-D-aspartate (NMDA) responses by carbachol (CCh) in the CA1 region of rat hippocampal slices. Carbachol 139-142 carbonic anhydrase 1 Rattus norvegicus 151-154 7510053-6 1993 These data suggest that low concentrations of CCh can acutely potentiate NMDA responses in the hippocampus by a Ca(2+)-sensitive process that is probably independent of both the activation of PKC and the release of Ca2+ from intracellular stores. Carbachol 46-49 carbonic anhydrase 2 Rattus norvegicus 215-218 8226807-4 1993 Inhibition of A beta secretion could also be effected by direct stimulation of m1 muscarinic acetylcholine receptors with carbachol. Carbachol 122-131 amyloid beta precursor protein Homo sapiens 14-20 8218298-2 1993 Insulin secretagogues, such as glucose and the muscarinic agonist carbachol, stimulate arachidonic acid accumulation, although the mechanisms involved are controversial: carbachol is believed to stimulate phospholipase A2, while glucose-induced arachidonic acid release is the result of diacylglycerol hydrolysis [Konrad, R. J., et al. Carbachol 66-75 insulin Homo sapiens 0-7 8218298-2 1993 Insulin secretagogues, such as glucose and the muscarinic agonist carbachol, stimulate arachidonic acid accumulation, although the mechanisms involved are controversial: carbachol is believed to stimulate phospholipase A2, while glucose-induced arachidonic acid release is the result of diacylglycerol hydrolysis [Konrad, R. J., et al. Carbachol 66-75 phospholipase A2 group IB Homo sapiens 205-221 8218298-2 1993 Insulin secretagogues, such as glucose and the muscarinic agonist carbachol, stimulate arachidonic acid accumulation, although the mechanisms involved are controversial: carbachol is believed to stimulate phospholipase A2, while glucose-induced arachidonic acid release is the result of diacylglycerol hydrolysis [Konrad, R. J., et al. Carbachol 170-179 insulin Homo sapiens 0-7 8218298-2 1993 Insulin secretagogues, such as glucose and the muscarinic agonist carbachol, stimulate arachidonic acid accumulation, although the mechanisms involved are controversial: carbachol is believed to stimulate phospholipase A2, while glucose-induced arachidonic acid release is the result of diacylglycerol hydrolysis [Konrad, R. J., et al. Carbachol 170-179 phospholipase A2 group IB Homo sapiens 205-221 8218298-9 1993 The combination of glucose and carbachol transiently activated Ca(2+)-dependent (but not Ca(2+)-independent) phospholipase A2 activity at 10 min, which corresponded to the peak of arachidonic acid release. Carbachol 31-40 phospholipase A2 group IB Homo sapiens 109-125 8293296-0 1993 Modulation of carbachol responsiveness in rat CA1 pyramidal neurons by corticosteroid hormones. Carbachol 14-23 carbonic anhydrase 1 Rattus norvegicus 46-49 8293296-2 1993 In this in vitro study we investigated if occupation of MRs or GRs affects the responsiveness of CA1 pyramidal neurons to the cholinergic analogue carbachol. Carbachol 147-156 carbonic anhydrase 1 Rattus norvegicus 97-100 7511424-2 1993 Acetylcholine and carbachol as well as nicotine decreased basal and hCG-induced T secretion. Carbachol 18-27 hypertrichosis 2 (generalised, congenital) Homo sapiens 68-71 8245971-2 1993 In the present study we show that carbachol (CCh) and Leu-Enkephalin act synergistically with vasoactive intestinal peptide (VIP) and corticotropin-releasing hormone (CRH), but not with l-isoproterenol, in increasing cyclic AMP formation. Carbachol 34-43 vasoactive intestinal peptide Rattus norvegicus 125-128 8245971-2 1993 In the present study we show that carbachol (CCh) and Leu-Enkephalin act synergistically with vasoactive intestinal peptide (VIP) and corticotropin-releasing hormone (CRH), but not with l-isoproterenol, in increasing cyclic AMP formation. Carbachol 34-43 corticotropin releasing hormone Rattus norvegicus 134-165 8245971-2 1993 In the present study we show that carbachol (CCh) and Leu-Enkephalin act synergistically with vasoactive intestinal peptide (VIP) and corticotropin-releasing hormone (CRH), but not with l-isoproterenol, in increasing cyclic AMP formation. Carbachol 34-43 corticotropin releasing hormone Rattus norvegicus 167-170 8245971-2 1993 In the present study we show that carbachol (CCh) and Leu-Enkephalin act synergistically with vasoactive intestinal peptide (VIP) and corticotropin-releasing hormone (CRH), but not with l-isoproterenol, in increasing cyclic AMP formation. Carbachol 45-48 vasoactive intestinal peptide Rattus norvegicus 125-128 8245971-2 1993 In the present study we show that carbachol (CCh) and Leu-Enkephalin act synergistically with vasoactive intestinal peptide (VIP) and corticotropin-releasing hormone (CRH), but not with l-isoproterenol, in increasing cyclic AMP formation. Carbachol 45-48 corticotropin releasing hormone Rattus norvegicus 134-165 8245971-2 1993 In the present study we show that carbachol (CCh) and Leu-Enkephalin act synergistically with vasoactive intestinal peptide (VIP) and corticotropin-releasing hormone (CRH), but not with l-isoproterenol, in increasing cyclic AMP formation. Carbachol 45-48 corticotropin releasing hormone Rattus norvegicus 167-170 8245971-6 1993 Substitution of the stable GTP analog guanosine 5"-O-(3"-thiotriphosphate) for GTP allows the CRH stimulation, but abolishes the CCh enhancement of both basal and CRH-stimulated enzyme activities. Carbachol 129-132 corticotropin releasing hormone Rattus norvegicus 163-166 8234284-4 1993 When injected into the AV3V region, the cholinergic drug carbachol induces natriuresis and the release of ANP. Carbachol 57-66 natriuretic peptide A Rattus norvegicus 106-109 8238525-6 1993 Stimulation of Caco-2 cell monolayers with phorbol myristate acetate or with the combination of carbachol and monolein was also associated with phosphorylation of the MARCKS protein, an endogenous substrate of PKC. Carbachol 96-105 myristoylated alanine rich protein kinase C substrate Homo sapiens 167-173 7693670-7 1993 Simultaneous incubation of carbachol with CCK or TPA increased the relative affinity of [3H]NMS binding, and the competitive inhibition curves showed a single class of carbachol binding site. Carbachol 27-36 cholecystokinin Homo sapiens 42-45 8246917-6 1993 The carbachol-elicited loss of m2- and m3-mAChR mRNA was blocked by their corresponding receptor subtype-specific antagonists, AF-DX 116 (m2-selective) and 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP) (m3-selective), and was concurrent with an increase in c-fos mRNA levels. Carbachol 4-13 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 268-273 7902747-5 1993 Under the same conditions, accumulations of radiolabelled IP1 and IP2 were reduced and those of GPI and IP3 unchanged in carbachol-treated cultures. Carbachol 121-130 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 58-61 7693670-7 1993 Simultaneous incubation of carbachol with CCK or TPA increased the relative affinity of [3H]NMS binding, and the competitive inhibition curves showed a single class of carbachol binding site. Carbachol 168-177 cholecystokinin Homo sapiens 42-45 7693670-11 1993 Incubation of acini with carbachol resulted in a decrease of [3H] NMS binding sites, and the addition of CCK or TPA caused an inhibition of the carbachol-induced disappearance of [3H]NMS binding sites. Carbachol 144-153 cholecystokinin Homo sapiens 105-108 8375509-3 1993 Both GTP gamma S and carbachol increase the Ca2+ sensitivity of myosin light chain kinase phosphorylation as well as light chain phosphorylation. Carbachol 21-30 myosin light chain kinase Homo sapiens 64-89 8415767-0 1993 Ambient glucose and aldose reductase-induced myo-inositol depletion modulate basal and carbachol-stimulated inositol phospholipid metabolism and diacylglycerol accumulation in human retinal pigment epithelial cells in culture. Carbachol 87-96 aldo-keto reductase family 1 member B Homo sapiens 20-36 7693670-12 1993 Finally, studies that examined the biological response of the acinar cells showed that biphasic amylase release in response to carbachol was completely suppressed by 10 nM CCK for entire range of carbachol. Carbachol 127-136 cholecystokinin Homo sapiens 172-175 7693670-12 1993 Finally, studies that examined the biological response of the acinar cells showed that biphasic amylase release in response to carbachol was completely suppressed by 10 nM CCK for entire range of carbachol. Carbachol 196-205 cholecystokinin Homo sapiens 172-175 7693670-13 1993 Taken together, these results suggest that the effect of CCK on carbachol-induced sequestration is important for the alteration of the apparent affinity of carbachol binding sites and the biological response of acinar cells to carbachol. Carbachol 64-73 cholecystokinin Homo sapiens 57-60 7693670-13 1993 Taken together, these results suggest that the effect of CCK on carbachol-induced sequestration is important for the alteration of the apparent affinity of carbachol binding sites and the biological response of acinar cells to carbachol. Carbachol 156-165 cholecystokinin Homo sapiens 57-60 7693670-13 1993 Taken together, these results suggest that the effect of CCK on carbachol-induced sequestration is important for the alteration of the apparent affinity of carbachol binding sites and the biological response of acinar cells to carbachol. Carbachol 156-165 cholecystokinin Homo sapiens 57-60 8143240-4 1993 However, in rings precontracted to a similar tone by endothelin-1, the relaxation elicited by carbachol was reduced in the vein but remained unchanged in the artery. Carbachol 94-103 endothelin-1 Oryctolagus cuniculus 53-65 8281323-2 1993 When Na+ currents were blocked with tetrodotoxin and K+ conductances known to be sensitive to suppression by muscarinic agonists were blocked by 2 mM Ba2+, CA1 cells were depolarized by carbachol (3-10 microM) with an attendant conductance increase, whereas prior to Ba2+ the agonist produced a decrease or no change in conductance. Carbachol 186-195 carbonic anhydrase 1 Homo sapiens 156-159 8268454-4 1993 At the highest dose of anti-IgE used, contraction reached 10.3 and 9.6% of the maximal carbachol contraction, respectively, as compared with 30.3% for solvent. Carbachol 87-96 immunoglobulin heavy constant epsilon Homo sapiens 28-31 8402579-2 1993 Carbachol and serum which act as growth factors for these cells induced a rapid, transient increase of intracellular Ca2+ concentration without a sustained increase. Carbachol 0-9 carbonic anhydrase 2 Homo sapiens 117-120 8237421-6 1993 Pre-incubation with vasoactive intestinal polypeptide (VIP), which coexists with acetylcholine in the parasympathetic neurons innervating the submandibular gland, increased the carbachol-induced tritium overflow significantly. Carbachol 177-186 vasoactive intestinal peptide Rattus norvegicus 20-53 8237421-6 1993 Pre-incubation with vasoactive intestinal polypeptide (VIP), which coexists with acetylcholine in the parasympathetic neurons innervating the submandibular gland, increased the carbachol-induced tritium overflow significantly. Carbachol 177-186 vasoactive intestinal peptide Rattus norvegicus 55-58 8237421-7 1993 The effect of VIP could be imitated by the adenylyl cyclase stimulator forskolin, which increased the carbachol-stimulated tritium efflux in a dose dependent manner. Carbachol 102-111 vasoactive intestinal peptide Rattus norvegicus 14-17 8255727-3 1993 CCh-evoked current oscillations were followed very closely by oscillations in intracellular free Ca2+ estimated from the Indo-1 signal, and were abolished by inclusion of EGTA in the pipette solution. Carbachol 0-3 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 97-100 8255727-7 1993 The present results are consistent with the hypothesis that CCh-evoked cationic current is gated by activation of a G protein and is steeply dependent on [Ca2+]i, fluctuations in the release of Ca2+ from stores during carbachol"s action produce oscillations in [Ca2+]i which cause similar oscillations in the cationic current. Carbachol 60-63 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 155-158 8255727-7 1993 The present results are consistent with the hypothesis that CCh-evoked cationic current is gated by activation of a G protein and is steeply dependent on [Ca2+]i, fluctuations in the release of Ca2+ from stores during carbachol"s action produce oscillations in [Ca2+]i which cause similar oscillations in the cationic current. Carbachol 60-63 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 194-197 8255727-7 1993 The present results are consistent with the hypothesis that CCh-evoked cationic current is gated by activation of a G protein and is steeply dependent on [Ca2+]i, fluctuations in the release of Ca2+ from stores during carbachol"s action produce oscillations in [Ca2+]i which cause similar oscillations in the cationic current. Carbachol 60-63 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 194-197 8255727-7 1993 The present results are consistent with the hypothesis that CCh-evoked cationic current is gated by activation of a G protein and is steeply dependent on [Ca2+]i, fluctuations in the release of Ca2+ from stores during carbachol"s action produce oscillations in [Ca2+]i which cause similar oscillations in the cationic current. Carbachol 218-227 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 155-158 8255727-7 1993 The present results are consistent with the hypothesis that CCh-evoked cationic current is gated by activation of a G protein and is steeply dependent on [Ca2+]i, fluctuations in the release of Ca2+ from stores during carbachol"s action produce oscillations in [Ca2+]i which cause similar oscillations in the cationic current. Carbachol 218-227 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 194-197 8255727-7 1993 The present results are consistent with the hypothesis that CCh-evoked cationic current is gated by activation of a G protein and is steeply dependent on [Ca2+]i, fluctuations in the release of Ca2+ from stores during carbachol"s action produce oscillations in [Ca2+]i which cause similar oscillations in the cationic current. Carbachol 218-227 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 194-197 8104089-0 1993 Bidirectional modulation of long-term potentiation by carbachol via M1 and M2 muscarinic receptors in guinea pig hippocampal mossy fiber-CA3 synapses. Carbachol 54-63 carbonic anhydrase 3 Cavia porcellus 137-140 8104089-4 1993 On the contrary, a high concentration (10 microM) of carbachol decreased the pre-tetanic amplitude of fEPSP, however, the magnitude of LTP was significantly larger than that in control slices in which pre-tetanic amplitude of fEPSP was reduced to the level of carbachol-treated slices by reducing the intensity of stimulation or extracellular Ca2+ concentration. Carbachol 53-62 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 343-346 8368310-6 1993 [4Cl-D-Phe6,Leu17]VIP (3.3 mg/kg), an antagonist to VIP, reduced basal, VIP-stimulated, and carbachol-stimulated HCO3- secretion. Carbachol 92-101 VIP peptides Cavia porcellus 18-21 8368310-6 1993 [4Cl-D-Phe6,Leu17]VIP (3.3 mg/kg), an antagonist to VIP, reduced basal, VIP-stimulated, and carbachol-stimulated HCO3- secretion. Carbachol 92-101 VIP peptides Cavia porcellus 52-55 8368310-6 1993 [4Cl-D-Phe6,Leu17]VIP (3.3 mg/kg), an antagonist to VIP, reduced basal, VIP-stimulated, and carbachol-stimulated HCO3- secretion. Carbachol 92-101 VIP peptides Cavia porcellus 52-55 8106077-6 1993 Somatostatin did not inhibit carbachol- or CCK-8-induced [Ca2+]i increase, but did inhibit carbachol- and CCK-8-induced pepsinogen secretion by 30 and 50%, respectively. Carbachol 91-100 somatostatin Homo sapiens 0-12 7693285-5 1993 ET-1 was a potent and effective contractile agonist in human bronchus, possessing similar potency and efficacy to leukotriene D4 (LTD4); EC50 (-log M): ET-1 = 7.76 +/- 0.09, n = 7; LTD4 = 8.46 +/- 0.53, n = 7; P > 0.2; maximum response (% 10 microM pre-carbachol): ET-1 = 103.8 +/- 17.4, n = 7; LTD4 = 95.5 +/- 9.3, n = 7; P > 0.6. Carbachol 256-265 endothelin 1 Homo sapiens 0-4 8101558-4 1993 Carbachol increased NO release in a concentration-dependent manner, with half-maximal stimulation at 173 microM (compared to 96 microM for direct activation of cyclic GMP formation). Carbachol 0-9 5'-nucleotidase, cytosolic II Homo sapiens 167-170 8102378-6 1993 SST (1 microM) and CLON (10 microM) reduced the Isc response to the muscarinic agonist, carbachol, by 60-70%; inhibition was reversed in pertussis toxin-treated cells. Carbachol 88-97 somatostatin Homo sapiens 0-3 8414920-7 1993 VIP (9 x 10(-9) mol/l) depolarized Vbl from -72 +/- 3 mV to -53 +/- 3 mV (n = 20) and CCH (10(-5) mol/l) from -62 +/- 3 to -35 +/- 4 mV (n = 10). Carbachol 86-89 vasoactive intestinal peptide Rattus norvegicus 0-3 8393663-1 1993 Treatment of CHO cells stably expressing the human M1 muscarinic acetylcholine (HM1) receptor with the cholinergic agonist carbachol results in a reduction in cellular levels of Gq alpha/G11 alpha. Carbachol 123-132 cholinergic receptor muscarinic 1 Homo sapiens 80-83 8503569-7 1993 There was a correlation between the percentage suppression of GM-CSF staining by inhaled corticosteroids and the percentage increase in FEV1 (r = 0.61, p < 0.05) and percentage decrease in carbachol responsiveness (r = 0.80, p < 0.01). Carbachol 192-201 colony stimulating factor 2 Homo sapiens 62-68 7692123-3 1993 The contractile force induced by 10(-6) M ET-1 was approximately 30% of that induced by 3 x 10(-5) M carbachol in detrusor muscle strips, approximately 40% of that induced by 10(-4) M norepinephrine in spermatic cord muscle strips and almost equal to that induced by 10(-5) M phenylephrine in prostatic adenoma muscle strips. Carbachol 101-110 endothelin 1 Homo sapiens 42-46 8233038-4 1993 The effects of L-AP3 was in contrast with those of a typical receptor antagonist, atropine; atropine inhibited carbachol-induced phosphoinositide hydrolysis, but the levels of [3H]PIs were not affected. Carbachol 111-120 leucine aminopeptidase 3 Rattus norvegicus 15-20 8347135-6 1993 Carbamylcholine induced a transient decrease in the gastric PAF level of normal rats, which was not associated with gastric erosion formation. Carbachol 0-15 PCNA clamp associated factor Rattus norvegicus 60-63 8102036-3 1993 Feeding or the cholinergic agonist carbachol increased plasma PP levels in conscious mice (+74 +/- 18 pg/ml vs. fasted mice and +141 +/- 17 pg/ml vs. control, respectively). Carbachol 35-44 pancreatic polypeptide Mus musculus 62-64 8102933-4 1993 In tracheal strips precontracted (60-70% of the maximum) with carbachol, ET-1 (1-100 nM) evoked slowly developing concentration-dependent relaxations. Carbachol 62-71 endothelin-1 Cavia porcellus 73-77 8407282-7 1993 On the contrary, Lyt 2.2+ cells were only able to respond to carbachol stimulus increasing cGMP levels and inositol phosphate formation while L3T4+ cells were unable to do it. Carbachol 61-70 nuclear factor of kappa light polypeptide gene enhancer in B cells 2, p49/p100 Mus musculus 17-20 8276492-0 1993 Endothelin-1 and carbachol: differences in contractile effects and myosin phosphorylation in lamb tracheal smooth muscle. Carbachol 17-26 myosin heavy chain 14 Homo sapiens 67-73 8276492-3 1993 Equimolar concentrations (10(-6)M) of endothelin 1 and carbachol elicit rapidly rising isometric tension which is maintained indefinitely in a steady state when fibres are stimulated with carbachol. Carbachol 188-197 endothelin 1 Homo sapiens 38-50 8103217-0 1993 PACAP and VIP stimulate enzyme secretion in rat pancreatic acini via interaction with VIP/PACAP-2 receptors: additive augmentation of CCK/carbachol-induced enzyme release. Carbachol 138-147 adenylate cyclase activating polypeptide 1 Rattus norvegicus 0-5 8103217-0 1993 PACAP and VIP stimulate enzyme secretion in rat pancreatic acini via interaction with VIP/PACAP-2 receptors: additive augmentation of CCK/carbachol-induced enzyme release. Carbachol 138-147 vasoactive intestinal peptide Rattus norvegicus 10-13 8103217-0 1993 PACAP and VIP stimulate enzyme secretion in rat pancreatic acini via interaction with VIP/PACAP-2 receptors: additive augmentation of CCK/carbachol-induced enzyme release. Carbachol 138-147 vasoactive intestinal peptide Rattus norvegicus 86-89 8103217-0 1993 PACAP and VIP stimulate enzyme secretion in rat pancreatic acini via interaction with VIP/PACAP-2 receptors: additive augmentation of CCK/carbachol-induced enzyme release. Carbachol 138-147 adenylate cyclase activating polypeptide 1 Rattus norvegicus 90-95 8103217-8 1993 Co-incubation of PACAP(1-27) or VIP with cholecystokinin-8 or carbachol revealed additive effects on enzyme secretion. Carbachol 62-71 adenylate cyclase activating polypeptide 1 Rattus norvegicus 17-22 8103217-8 1993 Co-incubation of PACAP(1-27) or VIP with cholecystokinin-8 or carbachol revealed additive effects on enzyme secretion. Carbachol 62-71 vasoactive intestinal peptide Rattus norvegicus 32-35 7688904-6 1993 or carbachol (100 microM, infusion) reduced ACh release and reduced the stimulation of ACh release by GAL with a magnitude corresponding to that of oxotremorine or carbachol alone. Carbachol 3-12 galanin and GMAP prepropeptide Rattus norvegicus 102-105 7688904-6 1993 or carbachol (100 microM, infusion) reduced ACh release and reduced the stimulation of ACh release by GAL with a magnitude corresponding to that of oxotremorine or carbachol alone. Carbachol 164-173 galanin and GMAP prepropeptide Rattus norvegicus 102-105 8499491-4 1993 Treatment of the cells with 10(-6) M bradykinin exhausts calcium release such that the successive treatment of the cells with norepinephrine, carbachol, or serotonin results in no secondary response. Carbachol 142-151 kininogen 1 Homo sapiens 37-47 8499491-5 1993 In contrast, bradykinin treatment of the cells following exposure to norepinephrine, carbachol, or serotonin caused a secondary increase in calcium release. Carbachol 85-94 kininogen 1 Homo sapiens 13-23 7684070-4 1993 Substance P increased the apparent affinity of the cholinergic agonists carbamylcholine and acetylcholine at equilibrium. Carbachol 72-87 tachykinin precursor 1 Homo sapiens 0-11 8501533-0 1993 C-fos expression in the pons and medulla of the cat during carbachol-induced active sleep. Carbachol 59-68 Fos proto-oncogene, AP-1 transcription factor subunit Felis catus 0-5 8501533-4 1993 On the other hand, the mean number of c-fos-expressing neurons found in the masseter, facial, and hypoglossal nuclei was lower in carbachol-injected than in control cats. Carbachol 130-139 Fos proto-oncogene, AP-1 transcription factor subunit Felis catus 38-43 8501533-6 1993 The specific sites in which there were greater numbers of c-fos-expressing neurons during active sleep-carbachol are discussed in relation to the state of active sleep, as well as the functional role that these sites play in generating the various physiological patterns of activity that occur during this state. Carbachol 103-112 Fos proto-oncogene, AP-1 transcription factor subunit Felis catus 58-63 7684070-7 1993 Substance P appeared to increase the rate of carbamylcholine-induced desensitization; however, the data are also consistent with an allosteric mechanism that does not involve the desensitized state. Carbachol 45-60 tachykinin precursor 1 Homo sapiens 0-11 8482444-9 1993 CONCLUSIONS: Intestinal macrophages produce a novel mucin secretagogue, which is as potent as carbachol for stimulating mucin secretion from colonic epithelial cells. Carbachol 94-103 LOC100508689 Homo sapiens 52-57 8510018-7 1993 In contrast to the contractile effect on longitudinal smooth muscle, ET-1 produced a potent concentration-dependent inhibition of both the spontaneous phasic activity and carbachol-stimulated activity of the circular smooth muscle. Carbachol 171-180 endothelin 1 Rattus norvegicus 69-73 8510018-10 1993 ET-1 also inhibited carbachol-induced increases in the phasic activity of circular smooth muscle with a similar potency. Carbachol 20-29 endothelin 1 Rattus norvegicus 0-4 8389541-2 1993 Stimulation of [3H]-inositol labeled pancreatic minilobules by buffer, bombesin, neuromedin B or carbachol in presence of 10 mM lithium, followed by separation of inositol phosphates, yielded the following results for cyclic inositol monophosphate (cIP) [DPM/mg protein; Mean +/- SEM (n)]: control [21 +/- 6, (9)]; bombesin [145 +/- 24, (12)]; neuromedin B (99 +/- 22 (9)] and carbachol [512 +/- 60, (12)]. Carbachol 377-386 neuromedin B Rattus norvegicus 81-93 8099469-8 1993 These studies support the conclusion that antibodies to the somatostatin complementary peptide demonstrate properties similar to those of somatostatin in that they inhibit carbachol-stimulated aminopyrine uptake and 125I-somatostatin binding. Carbachol 172-181 somatostatin Canis lupus familiaris 60-72 8099469-8 1993 These studies support the conclusion that antibodies to the somatostatin complementary peptide demonstrate properties similar to those of somatostatin in that they inhibit carbachol-stimulated aminopyrine uptake and 125I-somatostatin binding. Carbachol 172-181 somatostatin Canis lupus familiaris 138-150 8099469-8 1993 These studies support the conclusion that antibodies to the somatostatin complementary peptide demonstrate properties similar to those of somatostatin in that they inhibit carbachol-stimulated aminopyrine uptake and 125I-somatostatin binding. Carbachol 172-181 somatostatin Canis lupus familiaris 138-150 8097876-4 1993 Carbachol stimulated secretion of PST and SMT and intracellular Ca2+ mobilization in the range of 10(-6)-10(-4) M. The secretion and Ca2+ mobilization were inhibited by atropine, a muscarinic receptor antagonist. Carbachol 0-9 somatostatin Homo sapiens 42-45 8482444-9 1993 CONCLUSIONS: Intestinal macrophages produce a novel mucin secretagogue, which is as potent as carbachol for stimulating mucin secretion from colonic epithelial cells. Carbachol 94-103 LOC100508689 Homo sapiens 120-125 8335576-11 1993 These observations suggest that cooling induces from the epithelium the release of a cytochrome P-450-derived eicosanoid that potentiates contractions of the underlying airway smooth muscle to carbachol. Carbachol 193-202 Cytochrome P450 1A1 Canis lupus familiaris 85-101 8462791-9 1993 Pretreatment of isolated chief cells with motilin and erythromycin induced a reversible, dose- and time-dependent desensitization of the pepsinogen secretion stimulated by carbachol and cholecystokinin but not that stimulated by secretin, vasoactive intestinal peptide, or prostaglandin E2. Carbachol 172-181 motilin Homo sapiens 42-49 8320719-5 1993 Stimulation with carbachol shifted the distribution of cells to a more stellate morphology within 24 hr and later (after 48 hr) reduced the PKC substrate 80K/MARCKS by 22 +/- 7%. Carbachol 17-26 myristoylated alanine rich protein kinase C substrate Mus musculus 158-164 8479283-8 1993 Stimulation of mAChRs by the cholinergic agonist carbachol resulted in a pronounced increase in the intensity of 36G9 immunoreactivity, which may suggest that the mAChRs are functionally linked to the colocalized PKC gamma. Carbachol 49-58 protein kinase C, gamma Rattus norvegicus 213-222 7680902-2 1993 In the present study we treated pancreatic acini with carbachol to induce a complete loss of high-affinity CCK receptors and then examined the action of CCK-8 on inositol trisphosphate IP3(1,4,5), cytosolic calcium and amylase secretion in an effort to confirm and extend our previous hypothesis. Carbachol 54-63 cholecystokinin Homo sapiens 107-110 8455027-5 1993 The addition of carbachol to [3H]cytidine-prelabeled cells elicited a four- to fivefold increase in the accumulation of labeled CDP-DAG. Carbachol 16-25 cut like homeobox 1 Homo sapiens 128-131 8455027-7 1993 This result was in sharp contrast to findings in rat brain slices, in which carbachol-stimulated formation of [3H]CDP-DAG was potentiated approximately 10-fold by Li+ and substantially reduced by coincubation with inositol. Carbachol 76-85 cut like homeobox 1 Homo sapiens 114-117 8455027-8 1993 The formation of [3H]CDP-DAG in labeled SK-N-SH cells by carbachol was both concentration and time dependent. Carbachol 57-66 cut like homeobox 1 Homo sapiens 21-24 8455027-8 1993 The formation of [3H]CDP-DAG in labeled SK-N-SH cells by carbachol was both concentration and time dependent. Carbachol 57-66 hedgehog acyltransferase Homo sapiens 40-44 7680902-3 1993 We found that first incubating pancreatic acini with 10 mM carbachol decreased binding of 125I-CCK-8 measured during a second incubation by causing a complete loss of high-affinity CCK receptors with no change in the low-affinity CCK receptors. Carbachol 59-68 cholecystokinin Homo sapiens 95-98 7681672-0 1993 Substance P inhibits catecholamine biosynthesis stimulated by carbamylcholine in cultured adrenal chromaffin cells. Carbachol 62-77 tachykinin precursor 1 Bos taurus 0-11 8452524-1 1993 In an inositol-depleted 1321 N1 astrocytoma cell line, propranolol at 0.5 mM concentration and carbachol in the presence of Li+ induce a large increase (30-60-fold) in the amount of CMP-phosphatidate, the lipid substrate of PtdIns synthase. Carbachol 95-104 CDP-diacylglycerol--inositol 3-phosphatidyltransferase Homo sapiens 224-239 8452524-6 1993 There were two coincident peaks of carbachol-stimulated [3H]CMP-phosphatidate and PtdIns synthase activity, respectively. Carbachol 35-44 CDP-diacylglycerol--inositol 3-phosphatidyltransferase Homo sapiens 82-97 8452524-8 1993 The later peak, containing both carbachol-stimulated [3H]CMP-phosphatidate and PtdIns synthase activity, has a distribution in the gradient that is similar to NADPH-cytochrome c reductase activity, an endoplasmic-reticulum marker. Carbachol 32-41 CDP-diacylglycerol--inositol 3-phosphatidyltransferase Homo sapiens 79-94 8097916-5 1993 In addition, the increase in somatostatin secretion induced by incubation with veratridine (10(-4) M) or carbachol (10(-4) M) for 90 min was significantly reduced by the addition of caprylic acid, but somatostatin release stimulated by 5.6 x 10(-2) M KCl was not affected. Carbachol 105-114 somatostatin Rattus norvegicus 29-41 8097916-5 1993 In addition, the increase in somatostatin secretion induced by incubation with veratridine (10(-4) M) or carbachol (10(-4) M) for 90 min was significantly reduced by the addition of caprylic acid, but somatostatin release stimulated by 5.6 x 10(-2) M KCl was not affected. Carbachol 105-114 somatostatin Rattus norvegicus 201-213 7680902-3 1993 We found that first incubating pancreatic acini with 10 mM carbachol decreased binding of 125I-CCK-8 measured during a second incubation by causing a complete loss of high-affinity CCK receptors with no change in the low-affinity CCK receptors. Carbachol 59-68 cholecystokinin Homo sapiens 181-184 7680902-3 1993 We found that first incubating pancreatic acini with 10 mM carbachol decreased binding of 125I-CCK-8 measured during a second incubation by causing a complete loss of high-affinity CCK receptors with no change in the low-affinity CCK receptors. Carbachol 59-68 cholecystokinin Homo sapiens 181-184 8483799-4 1993 Carbachol dose-dependently stimulated the release of motilin from its producing cell. Carbachol 0-9 motilin Canis lupus familiaris 53-60 8095065-0 1993 Pairing the cholinergic agonist carbachol with patterned Schaffer collateral stimulation initiates protein synthesis in hippocampal CA1 pyramidal cell dendrites via a muscarinic, NMDA-dependent mechanism. Carbachol 32-41 carbonic anhydrase 1 Homo sapiens 132-135 8460100-6 1993 Carbachol (0.1 mM) increased the release of IAPP-LI at the lower glucose concentrations: 8.1 +/- 0.9, 8.7 +/- 0.6, and 11.7 +/- 1.8 fmol/islet/60 m in at 2, 7, and 20 mM glucose. Carbachol 0-9 islet amyloid polypeptide Rattus norvegicus 44-48 8383442-3 1993 Pretreatment of rabbit parietal cells with 10(-7) M rat TGF-alpha resulted in inhibition of both histamine- and carbachol-stimulated [14C]-aminopyrine (AP) accumulation. Carbachol 112-121 transforming growth factor alpha Rattus norvegicus 56-65 8464401-5 1993 In distal circular colon maximal inotropic response (10.1 +/- 2.1% of a maximal response to carbachol 10(-5) M) was found at PYY 10(-8) M; (ED50 3.1 +/- 1.2 x 10(-9) M). Carbachol 92-101 peptide YY Oryctolagus cuniculus 125-128 8381292-5 1993 After preconstriction with carbamylcholine, the TNF-alpha- and IL-1 beta-pretreated tissues produced a significant reduction in the maximal relaxation induced by isoproterenol, whereas the IL-2 pretreatment had no effect. Carbachol 27-42 tumor necrosis factor Cavia porcellus 48-57 8381292-5 1993 After preconstriction with carbamylcholine, the TNF-alpha- and IL-1 beta-pretreated tissues produced a significant reduction in the maximal relaxation induced by isoproterenol, whereas the IL-2 pretreatment had no effect. Carbachol 27-42 interleukin-1 beta Cavia porcellus 63-72 8382264-1 1993 Administration of carbachol, noradrenaline, and bradykinin induced Egr-1 mRNA expression within 1 h in mouse neuroblastoma x rat glioma hybrid NG108-15 cells. Carbachol 18-27 early growth response 1 Mus musculus 67-72 8382264-2 1993 With specific receptor antagonists, the Egr-1 inductions by carbachol and noradrenaline were shown to be mediated via cholinergic muscarinic and alpha 2-adrenergic receptors, respectively. Carbachol 60-69 early growth response 1 Mus musculus 40-45 8382264-5 1993 On the other hand, bradykinin consistently had an additive effect on Egr-1 induction, irrespective of the time of its addition, suggesting that the signal pathways for Egr-1 induction by carbachol or noradrenaline and by bradykinin are different. Carbachol 187-196 early growth response 1 Mus musculus 168-173 8382264-6 1993 Treatment of cells with pertussis toxin or cholera toxin strongly inhibited Egr-1 induction by carbachol or noradrenaline but only partially inhibited the induction by bradykinin. Carbachol 95-104 early growth response 1 Mus musculus 76-81 8093872-1 1993 We have examined the release of gastrin and somatostatin from the isolated perfused stomach of rats of three different age groups (4 months, 12 months, and 24 months old) in response to bombesin and carbachol. Carbachol 199-208 gastrin Rattus norvegicus 32-39 8093872-1 1993 We have examined the release of gastrin and somatostatin from the isolated perfused stomach of rats of three different age groups (4 months, 12 months, and 24 months old) in response to bombesin and carbachol. Carbachol 199-208 somatostatin Rattus norvegicus 44-56 8093872-4 1993 Bombesin (10(-10) and 10(-9) M) and carbachol (10(-8) and 10(-7) M) stimulated gastrin release in each age group. Carbachol 36-45 gastrin Rattus norvegicus 79-86 8093872-5 1993 The integrated release of gastrin in response to bombesin (10(-10) and 10(-9) M) or carbachol (10(-8) M) did not differ among the three age groups, although integrated gastrin release in response to carbachol (10(-7) M) decreased in 24-month-old rats. Carbachol 84-93 gastrin Rattus norvegicus 26-33 8093872-5 1993 The integrated release of gastrin in response to bombesin (10(-10) and 10(-9) M) or carbachol (10(-8) M) did not differ among the three age groups, although integrated gastrin release in response to carbachol (10(-7) M) decreased in 24-month-old rats. Carbachol 199-208 gastrin Rattus norvegicus 168-175 8093872-7 1993 Carbachol inhibited release of somatostatin in each age group. Carbachol 0-9 somatostatin Rattus norvegicus 31-43 8386783-3 1993 The purpose of this study was firstly to evaluate the interaction of cAMP-dependent agents (vasoactive intestinal polypeptide (VIP), norepinephrine (NE), and forskolin (FK)) on carbachol (Ca2+)-initiated motility and secondly to determine if a neural component of motility modulation existed by testing if the effect of cAMP-dependent agents was reversed by tetrodotoxin-induced neural blockade. Carbachol 177-186 VIP peptides Oryctolagus cuniculus 92-125 8386783-3 1993 The purpose of this study was firstly to evaluate the interaction of cAMP-dependent agents (vasoactive intestinal polypeptide (VIP), norepinephrine (NE), and forskolin (FK)) on carbachol (Ca2+)-initiated motility and secondly to determine if a neural component of motility modulation existed by testing if the effect of cAMP-dependent agents was reversed by tetrodotoxin-induced neural blockade. Carbachol 177-186 VIP peptides Oryctolagus cuniculus 127-130 8386783-6 1993 VIP, NE, and FK each caused a concentration-dependent inhibition of carbachol-stimulated phasic contractions. Carbachol 68-77 VIP peptides Oryctolagus cuniculus 0-3 8395431-1 1993 Smooth muscle cells isolated from the gastric muscle layers of the guinea pig were used to determine whether neurotensin can inhibit the contractile response produced by 10(-6) M carbachol by exerting a direct action on muscle cells. Carbachol 179-188 neurotensin/neuromedin N Cavia porcellus 109-120 8093872-8 1993 Compared with 4-month-old rats, the inhibition of somatostatin release by carbachol was less in 24-month-old rats at 10(-8) and 10(-7) M, and less in 12-month-old rats at 10(-7) M. The decreased basal gastrin secretion and well-preserved gastrin response were further confirmed in conscious aged rats tested by means of oral gavage with 10% peptone. Carbachol 74-83 somatostatin Rattus norvegicus 50-62 8093872-8 1993 Compared with 4-month-old rats, the inhibition of somatostatin release by carbachol was less in 24-month-old rats at 10(-8) and 10(-7) M, and less in 12-month-old rats at 10(-7) M. The decreased basal gastrin secretion and well-preserved gastrin response were further confirmed in conscious aged rats tested by means of oral gavage with 10% peptone. Carbachol 74-83 gastrin Rattus norvegicus 201-208 8093872-8 1993 Compared with 4-month-old rats, the inhibition of somatostatin release by carbachol was less in 24-month-old rats at 10(-8) and 10(-7) M, and less in 12-month-old rats at 10(-7) M. The decreased basal gastrin secretion and well-preserved gastrin response were further confirmed in conscious aged rats tested by means of oral gavage with 10% peptone. Carbachol 74-83 gastrin Rattus norvegicus 238-245 8109333-1 1993 In intact smooth muscle strips from chicken gizzard, carbachol elicited brief, phasic contractions which were associated with a very rapid, transient phosphorylation of the 20 kDa myosin light chains. Carbachol 53-62 myosin, heavy chain 15 Gallus gallus 180-186 8426908-6 1993 Rat TGF alpha inhibited carbachol stimulation throughout an agonist dose response. Carbachol 24-33 transforming growth factor alpha Rattus norvegicus 4-13 8426908-7 1993 Human TGF alpha was only effective in inhibiting carbachol if incubations were performed in the presence of air rather than 100% O2. Carbachol 49-58 transforming growth factor alpha Homo sapiens 6-15 8426908-8 1993 In air incubation, all three of the TGF alpha fragments inhibited carbachol stimulation but, in contrast to the effects on histamine, the peptides all were virtually equipotent with the native molecule. Carbachol 66-75 transforming growth factor alpha Homo sapiens 36-45 8426908-10 1993 The results suggest that there are pharmacological differences in the response of isolated parietal cells to TGF alpha-mediated inhibition of histamine and carbachol. Carbachol 156-165 transforming growth factor alpha Homo sapiens 109-118 8249680-11 1993 In a carbachol-induced stress model, insulin is not required for a putatively neural regulation of an increase in systemic glucose uptake but a "permissive" effect of insulin is essential. Carbachol 5-14 insulin Homo sapiens 37-44 8249680-11 1993 In a carbachol-induced stress model, insulin is not required for a putatively neural regulation of an increase in systemic glucose uptake but a "permissive" effect of insulin is essential. Carbachol 5-14 insulin Homo sapiens 167-174 7748314-6 1993 Carbachol (1 mM) induced a maximal c-fos mRNA response after 40 minutes in SH-SY5Y cells, an effect that could be mimicked through protein kinase C stimulation by phorbol esters. Carbachol 0-9 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 35-40 7684284-1 1993 In the cat, gastric lipase secretion was equally but weakly stimulated by pentagastrin, a major stimulant of acid secretion, and by carbamylcholine, a major stimulant of pepsin secretion. Carbachol 132-147 lipase F, gastric type Homo sapiens 12-26 8395431-3 1993 Neurotensin inhibited the contractile response produced by carbachol in a dose-dependent manner, with an IC50 value of 5 nM. Carbachol 59-68 neurotensin/neuromedin N Cavia porcellus 0-11 8510560-6 1993 Addition of GTP gamma S plus carbachol to sphincter muscle membranes prelabeled with [3H]inositol or 3H-arachidonic acid resulted in the formation of lysophosphatidylinositol and the liberation of arachidonic acid, thus suggesting the involvement of phospholipase A2. Carbachol 29-38 LOC104974671 Bos taurus 250-266 8510072-7 1993 The pharmacological response of functional mAChRs, determined as accumulation of inositol phosphates induced by carbachol, is greater in the medium containing IGF-I and insulin than that cultured in 0.1% BSA. Carbachol 112-121 insulin like growth factor 1 Canis lupus familiaris 159-164 1334091-1 1992 The histamine H3 receptor agonist (R)alpha-methylhistamine (MeHA) inhibited, in a nanomolar range, basal and carbachol-stimulated inositol phosphate formation in the human gastric tumoral cell line HGT1-clone 6. Carbachol 109-118 histamine receptor H3 Homo sapiens 4-25 1334091-1 1992 The histamine H3 receptor agonist (R)alpha-methylhistamine (MeHA) inhibited, in a nanomolar range, basal and carbachol-stimulated inositol phosphate formation in the human gastric tumoral cell line HGT1-clone 6. Carbachol 109-118 solute carrier family 25 member 16 Homo sapiens 198-202 1301240-6 1992 (ii) The cholinesterase-resistant agonist carbachol (10(-9)-2.5 x 10(-6) M) elicited a 2- to 3-fold greater negative chronotropic response in the aged compared with adult hearts. Carbachol 42-51 butyrylcholinesterase Rattus norvegicus 9-23 1359019-2 1992 Continuous exposure of the cells to carbachol or the nicotinic receptor agonist 1,1-dimethyl-4-phenylpiperazinium (DMPP) produces a time- and concentration-dependent increase in TH enzyme activity, whereas muscarine has no effect. Carbachol 36-45 tyrosine hydroxylase Bos taurus 178-180 1493543-1 1992 Thymopoietin, a polypeptide hormone isolated from thymus and involved in immune function, potently inhibited [125I]alpha-bungarotoxin binding to neonatal muscle cells in culture (IC50 = 3.8 nM) and blocked carbachol-stimulated 22Na uptake with an IC50 of 1.9 +/- 0.2 nM and 23 +/- 7 nM at a half-maximal and maximal concentration of carbachol, respectively. Carbachol 206-215 thymopoietin Homo sapiens 0-12 1493543-1 1992 Thymopoietin, a polypeptide hormone isolated from thymus and involved in immune function, potently inhibited [125I]alpha-bungarotoxin binding to neonatal muscle cells in culture (IC50 = 3.8 nM) and blocked carbachol-stimulated 22Na uptake with an IC50 of 1.9 +/- 0.2 nM and 23 +/- 7 nM at a half-maximal and maximal concentration of carbachol, respectively. Carbachol 333-342 thymopoietin Homo sapiens 0-12 1493543-4 1992 Thymopoietin did not appreciably alter myotube morphology on its own; however, it prevented the effects of nicotine and carbachol on muscle cell morphology at concentrations (1-10 nM) which corresponded well to those with which thymopoietin interacted at the receptor. Carbachol 120-129 thymopoietin Homo sapiens 0-12 1333428-6 1992 A 7.8% +/- 1.7% increase in mucin above basal levels after 24 hours was observed with a solid-phase immunoassay in control wells, whereas histamine, gastrin, and carbamylcholine increased total mucin by 14% +/- 0.7%, 17% +/- 4.3%, and 20.4% +/- 4%, respectively (all P < 0.01), and PGE2 had no significant effect. Carbachol 162-177 mucin Canis lupus familiaris 194-199 1333428-8 1992 The acid secretagogues histamine, gastrin, and carbamylcholine stimulated mucin synthesis and PC release. Carbachol 47-62 mucin Canis lupus familiaris 74-79 1288495-0 1992 The cholinergic agonist carbachol reduces intracellular beta-amyloid precursor protein in PC 12 and C6 cells. Carbachol 24-33 amyloid beta precursor protein Rattus norvegicus 56-86 1475305-4 1992 We now report the effects of carbachol microinjected into CA1, the DG, or the ventral subiculum (VS) on acoustic startle and PPI. Carbachol 29-38 carbonic anhydrase 1 Rattus norvegicus 58-61 1475305-5 1992 Carbachol infusion into CA1 or the DG depressed startle. Carbachol 0-9 carbonic anhydrase 1 Rattus norvegicus 24-27 1475305-6 1992 Carbachol infusion decreased PPI with a regional rank-order potency CA1 > DG > VS. CA1 infusions more potently depressed the startle reflex. Carbachol 0-9 carbonic anhydrase 1 Rattus norvegicus 68-71 1331066-3 1992 Carbachol-mediated activation of the Hm1 receptor in the 39M1-81 clone, which is not a mitogenic signal, produced a similarly rapid although greater activation of PLD. Carbachol 0-9 cholinergic receptor muscarinic 1 Homo sapiens 37-40 1279984-4 1992 The inhibitory effect of neurotensin during CCH stimulation was blocked concentration dependently in the presence of the K(+)-channel blocker apamin. Carbachol 44-47 neurotensin Rattus norvegicus 25-36 1279984-8 1992 After depletion of internal Ca2+ stores by repetitive stimulation with CCH and caffeine in Ca(2+)-free buffer, reintroduction of external Ca2+ restored neurotensin inhibition of the contraction induced by CCH. Carbachol 205-208 neurotensin Rattus norvegicus 152-163 1443219-1 1992 In conscious, unrestrained rats, the intracerebroventricular injection of the cholinergic agonist, carbachol, or angiotensin II resulted in the transient stimulation of vasopressin secretion, elevation of mean arterial blood pressure, and reduction of heart rate. Carbachol 99-108 arginine vasopressin Rattus norvegicus 169-180 1443219-2 1992 After the injection of carbachol (25 ng) into a lateral cerebral ventricle, the plasma vasopressin concentration in male rats was increased to twice that of female rats in each phase of the estrous cycle; mean arterial blood pressure was elevated more in males than females, whereas heart rate fell to the same extent in both sexes. Carbachol 23-32 arginine vasopressin Rattus norvegicus 87-98 1282072-13 1992 The NK1-selective antagonist (+/-)-CP-96,345 also inhibited responses of the rat bladder and guinea-pig ileum to substance P methyl ester; however, in the rat bladder at 1 microM, this antagonist reversibly inhibited responses both to the NK2-selective agonist [beta-Ala8]-NKA(4-10) and to the muscarinic agonist carbachol (P < or = 0.01), thus showing evidence of some non-selective depressant actions. Carbachol 313-322 tachykinin receptor 1 Homo sapiens 4-7 1306243-3 1992 IL-2 enhanced carbachol (Carb)- and KCl-induced contraction, and attenuated isoproterenol (Iso)-induced relaxation. Carbachol 14-23 interleukin-2 Cavia porcellus 0-4 1306243-3 1992 IL-2 enhanced carbachol (Carb)- and KCl-induced contraction, and attenuated isoproterenol (Iso)-induced relaxation. Carbachol 25-29 interleukin-2 Cavia porcellus 0-4 1331066-3 1992 Carbachol-mediated activation of the Hm1 receptor in the 39M1-81 clone, which is not a mitogenic signal, produced a similarly rapid although greater activation of PLD. Carbachol 0-9 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 163-166 1331066-5 1992 In each case, the stimulation of PLD correlated closely with the ability to stimulate inositol phospholipid breakdown and was entirely dependent on the activation of protein kinase C. Moreover, the ability of both thrombin and carbachol to stimulate PLD was found to be rapidly desensitized, with a similar time course of desensitization (t1/2 desensitization, 90 s). Carbachol 227-236 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 33-36 1331066-5 1992 In each case, the stimulation of PLD correlated closely with the ability to stimulate inositol phospholipid breakdown and was entirely dependent on the activation of protein kinase C. Moreover, the ability of both thrombin and carbachol to stimulate PLD was found to be rapidly desensitized, with a similar time course of desensitization (t1/2 desensitization, 90 s). Carbachol 227-236 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 250-253 1331066-7 1992 In this regard, in addition to stimulation of PLD, thrombin and carbachol were both able to stimulate the activity of a phosphocholine-specific phospholipase C (PC-PLC), which did not appear to desensitize within the time course employed. Carbachol 64-73 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 46-49 1361226-0 1992 Effect of carbachol on luminal release of somatostatin from isolated perfused rat duodenum. Carbachol 10-19 somatostatin Rattus norvegicus 42-54 1445347-1 1992 We measured dose-response curves for carbachol stimulation of phosphatidyl inositol (PI) turnover with mutants of the Hm1 muscarinic cholinergic receptor having various deletions from amino acids 219 to 358 of the large third intracellular (i3) loop (208 to 366). Carbachol 37-46 cholinergic receptor muscarinic 1 Homo sapiens 118-121 1331363-2 1992 Activation of the nAChR by carbachol increased extracellular adenosine concentration in a dose-dependent manner. Carbachol 27-36 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 18-23 1331363-8 1992 We found that the adenosine receptor antagonist, BW1434U, significantly inhibited carbachol-induced nAChR desensitization, indicating that extracellular adenosine is involved in nAChR desensitization. Carbachol 82-91 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 100-105 1331363-8 1992 We found that the adenosine receptor antagonist, BW1434U, significantly inhibited carbachol-induced nAChR desensitization, indicating that extracellular adenosine is involved in nAChR desensitization. Carbachol 82-91 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 178-183 1279850-3 1992 Furthermore, the pancreas of the rats showed good exocrine secretion of enzymes such as amylase and lipase on stimulation with carbachol in vitro. Carbachol 127-136 lipase G, endothelial type Rattus norvegicus 100-106 1361226-4 1992 The ratio of somatostatin-14 to somatostatin-28 in picograms was about 1 during basal release but increased to approximately 2 during carbachol stimulation. Carbachol 134-143 somatostatin Rattus norvegicus 13-25 1450914-0 1992 Central carbachol stimulates vasopressin release into interstitial fluid adjacent to the paraventricular nucleus. Carbachol 8-17 arginine vasopressin Rattus norvegicus 29-40 1445294-1 1992 Wortmannin, a specific inhibitor of myosin light chain kinase (MLCK), enhanced carbachol-induced formation of [3H]phosphatidylethanol ([3H]PEt), a marker of phospholipase D (PLD) activity, in [3H]palmitic acid-labeled PC12 cells. Carbachol 79-88 myosin light chain kinase Rattus norvegicus 36-61 1445294-1 1992 Wortmannin, a specific inhibitor of myosin light chain kinase (MLCK), enhanced carbachol-induced formation of [3H]phosphatidylethanol ([3H]PEt), a marker of phospholipase D (PLD) activity, in [3H]palmitic acid-labeled PC12 cells. Carbachol 79-88 myosin light chain kinase Rattus norvegicus 63-67 1417779-0 1992 Carbachol stimulation of phospholipase A2 and insulin secretion in pancreatic islets. Carbachol 0-9 phospholipase A2 group IB Homo sapiens 25-41 1417779-6 1992 Carbachol stimulation of arachidonic acid release is mediated by activation of phospholipase A2, as demonstrated by early increases in endogenous lysophosphatidylcholine. Carbachol 0-9 phospholipase A2 group IB Homo sapiens 79-95 1417779-7 1992 In addition to phospholipase A2 activation, carbachol-induced arachidonic acid accumulation also appears to involve diacylglycerol hydrolysis, since the diacylglycerol lipase inhibitor RG80267 partly inhibited arachidonic acid accumulation. Carbachol 44-53 phospholipase A2 group IB Homo sapiens 15-31 1329743-4 1992 Interestingly, carbachol, but not PDGF, induces an increase in tyrosine phosphorylation of the p125FAK and p130 v-src substrates. Carbachol 15-24 protein tyrosine kinase 2 Homo sapiens 95-102 1395777-1 1992 To visualize ASM contraction in vitro, we measured changes in cross-sectional area and inner circumference of isolated porcine and human bronchi in response to acetylcholine or carbamylcholine chloride (Carbachol) using high-frequency ultrasound. Carbachol 177-201 H19 imprinted maternally expressed transcript Homo sapiens 13-16 1450914-1 1992 We have used an in vivo double microdialysis probe technique in conscious rats to determine whether the application of carbachol to one paraventricular nucleus (PVN) can result in increased local release of vasopressin from that PVN. Carbachol 119-128 arginine vasopressin Rattus norvegicus 207-218 1450914-4 1992 When carbachol (100 micrograms/ml) was included in the perfusate of one probe for the first 10 min of a 30-min collection period, while the other probe continued to be perfused with saline alone, there was a seven-fold increase in the concentration of vasopressin in the dialysate from the carbachol-perfused probe; the vasopressin concentration in the dialysate from the contralateral probe increased only slightly. Carbachol 5-14 arginine vasopressin Rattus norvegicus 252-263 1328861-2 1992 To determine whether the increases in c-fos and c-jun mRNA induced by carbachol and thrombin are sufficient to stimulate AP-1-mediated transactivation, 1321N1 cells were transfected with a reporter carrying two copies of the tetradecanoyl phorbol acetate response element and the firefly luciferase gene. Carbachol 70-79 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 38-43 1328861-2 1992 To determine whether the increases in c-fos and c-jun mRNA induced by carbachol and thrombin are sufficient to stimulate AP-1-mediated transactivation, 1321N1 cells were transfected with a reporter carrying two copies of the tetradecanoyl phorbol acetate response element and the firefly luciferase gene. Carbachol 70-79 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 48-53 1328861-2 1992 To determine whether the increases in c-fos and c-jun mRNA induced by carbachol and thrombin are sufficient to stimulate AP-1-mediated transactivation, 1321N1 cells were transfected with a reporter carrying two copies of the tetradecanoyl phorbol acetate response element and the firefly luciferase gene. Carbachol 70-79 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 121-125 1450914-4 1992 When carbachol (100 micrograms/ml) was included in the perfusate of one probe for the first 10 min of a 30-min collection period, while the other probe continued to be perfused with saline alone, there was a seven-fold increase in the concentration of vasopressin in the dialysate from the carbachol-perfused probe; the vasopressin concentration in the dialysate from the contralateral probe increased only slightly. Carbachol 5-14 arginine vasopressin Rattus norvegicus 320-331 1450914-6 1992 When one of the paired probes was perfused with carbachol (100 micrograms/ml) for 30 min, there were similar increases in the concentration of vasopressin in the dialysate from both probes and a sustained increase in the plasma vasopressin concentration. Carbachol 48-57 arginine vasopressin Rattus norvegicus 143-154 1450914-6 1992 When one of the paired probes was perfused with carbachol (100 micrograms/ml) for 30 min, there were similar increases in the concentration of vasopressin in the dialysate from both probes and a sustained increase in the plasma vasopressin concentration. Carbachol 48-57 arginine vasopressin Rattus norvegicus 228-239 1415544-0 1992 CCK, bombesin, and carbachol stimulate c-fos, c-jun, and c-myc oncogene expression in rat pancreatic acini. Carbachol 19-28 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 39-44 1505686-2 1992 The binding and interaction of carbamoylcholine with the nicotinic acetylcholine receptor was investigated using photolytically released carbamoylcholine ("caged" carbamoylcholine). Carbachol 31-47 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 57-89 1505686-2 1992 The binding and interaction of carbamoylcholine with the nicotinic acetylcholine receptor was investigated using photolytically released carbamoylcholine ("caged" carbamoylcholine). Carbachol 137-153 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 57-89 1505686-2 1992 The binding and interaction of carbamoylcholine with the nicotinic acetylcholine receptor was investigated using photolytically released carbamoylcholine ("caged" carbamoylcholine). Carbachol 137-153 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 57-89 1328861-3 1992 Thrombin was significantly more effective than carbachol at stimulating AP-1-mediated transactivation. Carbachol 47-56 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 72-76 1328861-4 1992 To identify the factors underlying the difference in AP-1 activity induced by carbachol and thrombin, members of the fos and jun families which encode components of AP-1 were examined. Carbachol 78-87 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 53-57 1328861-5 1992 Carbachol and thrombin have similar effects on expression of c-fos, fosB, fra-2, junB, and junD, both acutely and over a 24-h time course. Carbachol 0-9 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 61-66 1328861-5 1992 Carbachol and thrombin have similar effects on expression of c-fos, fosB, fra-2, junB, and junD, both acutely and over a 24-h time course. Carbachol 0-9 FosB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 68-72 1328861-5 1992 Carbachol and thrombin have similar effects on expression of c-fos, fosB, fra-2, junB, and junD, both acutely and over a 24-h time course. Carbachol 0-9 FOS like 2, AP-1 transcription factor subunit Homo sapiens 74-79 1328861-5 1992 Carbachol and thrombin have similar effects on expression of c-fos, fosB, fra-2, junB, and junD, both acutely and over a 24-h time course. Carbachol 0-9 JunB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 81-85 1328861-5 1992 Carbachol and thrombin have similar effects on expression of c-fos, fosB, fra-2, junB, and junD, both acutely and over a 24-h time course. Carbachol 0-9 JunD proto-oncogene, AP-1 transcription factor subunit Homo sapiens 91-95 1328861-6 1992 However, whereas carbachol leads only to transient induction of c-jun (maximal at 0.5 h), thrombin induces a biphasic increase in c-jun mRNA--an initial peak at 0.5 h and a second, more-prolonged increase at 12 h. Thrombin but not carbachol also induces a late increase in fra-1 mRNA, which peaks at 12 h. The secondary increase in c-jun mRNA is associated with marked increases in c-Jun protein levels and AP-1 DNA-binding activity. Carbachol 17-26 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 64-69 1328861-6 1992 However, whereas carbachol leads only to transient induction of c-jun (maximal at 0.5 h), thrombin induces a biphasic increase in c-jun mRNA--an initial peak at 0.5 h and a second, more-prolonged increase at 12 h. Thrombin but not carbachol also induces a late increase in fra-1 mRNA, which peaks at 12 h. The secondary increase in c-jun mRNA is associated with marked increases in c-Jun protein levels and AP-1 DNA-binding activity. Carbachol 231-240 coagulation factor II, thrombin Homo sapiens 90-98 1415544-0 1992 CCK, bombesin, and carbachol stimulate c-fos, c-jun, and c-myc oncogene expression in rat pancreatic acini. Carbachol 19-28 MYC proto-oncogene, bHLH transcription factor Rattus norvegicus 57-62 1330669-4 1992 EGF, isoproterenol and NECA have no effect on basal levels of inositol phosphates (InsPs) in human RPE cells, but EGF specifically elevates the carbachol-induced stimulation of InsPs. Carbachol 144-153 epidermal growth factor Homo sapiens 0-3 1330669-4 1992 EGF, isoproterenol and NECA have no effect on basal levels of inositol phosphates (InsPs) in human RPE cells, but EGF specifically elevates the carbachol-induced stimulation of InsPs. Carbachol 144-153 epidermal growth factor Homo sapiens 114-117 1437521-2 1992 Vasoactive intestinal polypeptide (VIP) produced a dose-dependent depolarisation (EC50 = 30 nM) and an increase in membrane conductance that could be potentiated by carbachol. Carbachol 165-174 VIP peptides Cavia porcellus 0-33 1340058-3 1992 Using this assay, high doses of cholecystokinin or carbachol were found to stimulate the intracellular conversion of at least three zymogens (procarboxypeptidase A1, procarboxypeptidase B, and chymotrypsinogen 2) to their active forms. Carbachol 51-60 carboxypeptidase B1 Homo sapiens 166-187 1382539-4 1992 Pretreatment of subjects with the selective histamine H1-receptor antagonist cetirizine, 10 mg orally 4 h before intradermal injections, inhibited vasodilatation caused by the intradermal injection of histamine (750 pmol), endothelin-1 (63 pmol), and carbachol (750 pmol). Carbachol 251-260 histamine receptor H1 Homo sapiens 44-65 1436659-3 1992 Stimulation of bovine adrenal medulla by carbamoylcholine chloride induced the secretion of PC1 and PC2. Carbachol 41-66 proprotein convertase subtilisin/kexin type 1 Bos taurus 92-95 1436659-3 1992 Stimulation of bovine adrenal medulla by carbamoylcholine chloride induced the secretion of PC1 and PC2. Carbachol 41-66 proprotein convertase subtilisin/kexin type 2 Bos taurus 100-103 1437521-2 1992 Vasoactive intestinal polypeptide (VIP) produced a dose-dependent depolarisation (EC50 = 30 nM) and an increase in membrane conductance that could be potentiated by carbachol. Carbachol 165-174 VIP peptides Cavia porcellus 35-38 1332901-1 1992 I. v. administration of carbachol or neostigmine produced a dose-dependent decrease in the angiotensin I pressor response and in conversion of angiotensin I to angiotensin II in anesthetized rats. Carbachol 24-33 angiotensinogen Rattus norvegicus 160-174 1514577-1 1992 In rat pancreatic and submandibular gland acini during exposure to carbachol, changes in the fluorescence emission intensity ratio (R) of acini loaded with mag-fura-2 resemble changes in cytosolic Ca2+ concentration (Ca2+i) in acini loaded with fura-2. Carbachol 67-76 carbonic anhydrase 2 Rattus norvegicus 197-200 1514577-7 1992 In acini from either gland, 1,2-bis(2-aminophenoxy)ethan-N,N,N",N"-tetraacetic acid (BAPTA) suppressed carbachol-induced Ca2+i transients. Carbachol 103-112 carbonic anhydrase 2 Rattus norvegicus 121-124 1321772-5 1992 PACAP resembled VIP in that it stimulated a secretory response potentiated by carbachol, inhibited by bumetanide and barium chloride, and not further stimulated by the subsequent addition of VIP. Carbachol 78-87 adenylate cyclase activating polypeptide 1 Homo sapiens 0-5 1359631-4 1992 Both intra-arterial carbachol (10(-6) M) and GRP (10(-8) M) increased acid secretion and inhibited somatostatin secretion. Carbachol 20-29 somatostatin Homo sapiens 99-111 1641762-8 1992 Perfusion with 10 nmol/L VIP decreased basal tone and completely abolished the contraction induced by 100 nmol/L carbachol. Carbachol 113-122 VIP peptides Oryctolagus cuniculus 25-28 1641762-9 1992 VIP, CGRP, and somatostatin were released from the LES in response to 10 nmol/L carbachol (VIP rose from 55 +/- 13 to 179 +/- 24 pmol/L, CGRP, from 114 +/- 30 to 239 +/- 33 pmol/L, and somatostatin from 15 +/- 2 to 27 +/- 4 pmol/L; all p less than 0.001). Carbachol 80-89 VIP peptides Oryctolagus cuniculus 0-3 1641762-9 1992 VIP, CGRP, and somatostatin were released from the LES in response to 10 nmol/L carbachol (VIP rose from 55 +/- 13 to 179 +/- 24 pmol/L, CGRP, from 114 +/- 30 to 239 +/- 33 pmol/L, and somatostatin from 15 +/- 2 to 27 +/- 4 pmol/L; all p less than 0.001). Carbachol 80-89 VIP peptides Oryctolagus cuniculus 91-94 1381656-0 1992 Substance P inhibits carbamylcholine-stimulated 22Na+ efflux from acetylcholine receptor-enriched Torpedo electroplaque membrane vesicles. Carbachol 21-36 tachykinin precursor 1 Homo sapiens 0-11 1637359-3 1992 The electrical response to carbachol coincided with a transient translocation of PKC alpha from the soluble to the particulate fraction. Carbachol 27-36 protein kinase C alpha Homo sapiens 81-90 1637359-4 1992 The carbachol-, PDB- and 8-Br-cAMP-induced ISC responses were inhibited by pretreatment of the cells with PMA (0.5 microM) for 2 h, a time period in which PKC alpha, beta 1 and gamma levels were not changed. Carbachol 4-13 protein kinase C alpha Homo sapiens 155-164 1637359-4 1992 The carbachol-, PDB- and 8-Br-cAMP-induced ISC responses were inhibited by pretreatment of the cells with PMA (0.5 microM) for 2 h, a time period in which PKC alpha, beta 1 and gamma levels were not changed. Carbachol 4-13 potassium calcium-activated channel subfamily M regulatory beta subunit 1 Homo sapiens 166-182 1330588-5 1992 Airway responsiveness was measured before and 24 h after challenge by determining the dose of inhaled carbachol that caused a 400% increase in SRL (PD400%). Carbachol 102-111 sarcalumenin Ovis aries 143-146 1636673-1 1992 We investigated the role of protein kinase C (PKC) in mediating carbachol"s stimulation of transepithelial Cl- secretion in T84 cells. Carbachol 64-73 proline rich transmembrane protein 2 Homo sapiens 28-44 1636673-1 1992 We investigated the role of protein kinase C (PKC) in mediating carbachol"s stimulation of transepithelial Cl- secretion in T84 cells. Carbachol 64-73 proline rich transmembrane protein 2 Homo sapiens 46-49 1636673-3 1992 Carbachol stimulated PKC activity, suggesting that the enzyme might participate in the hormone"s action. Carbachol 0-9 proline rich transmembrane protein 2 Homo sapiens 21-24 1636673-5 1992 The effect of DMIs was not due to amplification of carbachol-induced increases in PKC activity, however; PKC activity during carbachol stimulation was no higher in the presence of DMIs than in their absence. Carbachol 125-134 proline rich transmembrane protein 2 Homo sapiens 105-108 1636673-6 1992 Augmentation of carbachol"s action by DMIs appeared to be due to the direct activation of PKC which, like PMA, stimulated the Cl- conductance of the apical membrane (GCl). Carbachol 16-25 proline rich transmembrane protein 2 Homo sapiens 90-93 1636673-6 1992 Augmentation of carbachol"s action by DMIs appeared to be due to the direct activation of PKC which, like PMA, stimulated the Cl- conductance of the apical membrane (GCl). Carbachol 16-25 germ cell-less 2, spermatogenesis associated Homo sapiens 166-169 1636673-7 1992 The effects of DMIs and carbachol on GCl were additive. Carbachol 24-33 germ cell-less 2, spermatogenesis associated Homo sapiens 37-40 1636673-8 1992 Carbachol itself stimulated GCl but not by activating PKC; staurosporine did not blunt the effect of carbachol on GCl. Carbachol 0-9 germ cell-less 2, spermatogenesis associated Homo sapiens 28-31 1330841-3 1992 The increase in inositol phospholipid hydrolysis induced by repeated addition of CDP-choline was obliterated when cultures were incubated in the presence of the muscarinic receptor agonist, carbamylcholine. Carbachol 190-205 cut-like homeobox 1 Rattus norvegicus 81-84 1319463-7 1992 The CCh-induced [Ca2+]i elevation was concentration-dependently inhibited by pirenzepine and 4-diphenylacetoxy-N-methylpiperidine methiodide, two rather selective antagonists of M1 and M3 muscarinic receptor subtypes, respectively, whereas methoctramine, an M2 antagonist, was ineffective. Carbachol 4-7 cholinergic receptor muscarinic 3 Homo sapiens 185-207 1331918-6 1992 In addition, chronic treatment (24 hrs) of SK-N-SH cells with pertussis toxin blocked 75% of the stimulatory effects of carbachol, but inhibited only 38% of the paraoxon-induced response. Carbachol 120-129 hedgehog acyltransferase Homo sapiens 43-47 1352680-7 1992 When either dibutyryl cAMP, forskolin or theophylline was added in culture medium with A23187, phorbol ester or carbachol, a synergistic effect was found on pancreastatin and somatostatin secretion. Carbachol 112-121 somatostatin Homo sapiens 175-187 1376916-11 1992 In muscle strips obtained from bladders kept empty in vivo, PTHrP-(1-34)-NH2 relaxed carbachol-induced contraction in a dose-dependent manner but failed to relax the contraction in muscle strips from distended bladders that had high endogenous PTHrP expression. Carbachol 85-94 parathyroid hormone-like hormone Rattus norvegicus 60-65 1378274-3 1992 IC50 of CP 96,345 for binding of [125I]-BH-SP and for SP-induced (3 x 10(-9) M) rise of [Ca2+]i were about 10(-9) M. CP-96,345 neither affected binding of [125I]-labelled Bolton-Hunter cholecystokinin octapeptide ([125I]-BH-CCK-8) nor the [Ca2+]i responses to CCK-8, carbamylcholine or bombesin. Carbachol 267-282 tachykinin precursor 1 Homo sapiens 54-56 1393268-22 1992 Detrusor preparations contracted by carbachol were concentration-dependently relaxed by exogenously administered NO, SIN-1 (NO-donor), and isoprenaline, whereas vasoactive intestinal polypeptide had minor effects. Carbachol 36-45 MAPK associated protein 1 Homo sapiens 117-122 1393268-23 1992 NO and SIN-1 induced maximal relaxations of 63 +/- 3% and 70 +/- 4%, respectively, of the tension induced by carbachol. Carbachol 109-118 MAPK associated protein 1 Homo sapiens 7-12 1511949-1 1992 The stimulation of the secretion of insulin and pancreatic polypeptide (PP) by carbachol, three different neuropeptides (gastrin-releasing peptide (GRP), vasoactive intestinal polypeptide (VIP) and neuromedin C (NC)), and glucose was investigated using the isolated perfused ventral part of the rat pancreas, with or without atropine. Carbachol 79-88 pancreatic polypeptide Rattus norvegicus 72-74 1423494-8 1992 Furthermore, PACAP-38 (7 nmol/kg) potentiated the plasma glucagon response to the cholinergic agonist carbachol (0.16 mumol/kg). Carbachol 102-111 adenylate cyclase activating polypeptide 1 Mus musculus 13-18 1423494-10 1992 Moreover, PACAP-38 inhibited a carbachol-induced increase in the level of plasma insulin in the absence but not in the presence of adrenergic blockade. Carbachol 31-40 adenylate cyclase activating polypeptide 1 Mus musculus 10-15 1423494-12 1992 We conclude that PACAP-like immunoreactivity exists in nerve fibers innervating the mouse and rat pancreas, particularly the intrapancreatic ganglia, and that PACAP-38 augments both basal and carbachol-stimulated glucagon secretion in the mouse. Carbachol 192-201 adenylate cyclase activating polypeptide 1 Mus musculus 159-164 1511949-3 1992 In addition, we studied the secretion of insulin and PP from the dorsal part of the rat pancreas in response to GRP and carbachol. Carbachol 120-129 pancreatic polypeptide Rattus norvegicus 53-55 1319016-6 1992 Acute (36-h) treatment with carbachol also caused an increase in VIP immunoreactivity (about 2-fold, and blocked by atropine) without an increase in VIP mRNA level. Carbachol 28-37 vasoactive intestinal peptide Homo sapiens 65-68 1511949-4 1992 The secretion of insulin and PP in response to carbachol and GRP were dose-dependent in range of 10(-9) M to 10(-6) M. The responses of insulin and PP to carbachol (10(-7) M) were biphasic, and were abolished by atropine (10(-5) M). Carbachol 47-56 pancreatic polypeptide Rattus norvegicus 29-31 1511949-4 1992 The secretion of insulin and PP in response to carbachol and GRP were dose-dependent in range of 10(-9) M to 10(-6) M. The responses of insulin and PP to carbachol (10(-7) M) were biphasic, and were abolished by atropine (10(-5) M). Carbachol 47-56 pancreatic polypeptide Rattus norvegicus 148-150 1511949-4 1992 The secretion of insulin and PP in response to carbachol and GRP were dose-dependent in range of 10(-9) M to 10(-6) M. The responses of insulin and PP to carbachol (10(-7) M) were biphasic, and were abolished by atropine (10(-5) M). Carbachol 154-163 pancreatic polypeptide Rattus norvegicus 29-31 1511949-4 1992 The secretion of insulin and PP in response to carbachol and GRP were dose-dependent in range of 10(-9) M to 10(-6) M. The responses of insulin and PP to carbachol (10(-7) M) were biphasic, and were abolished by atropine (10(-5) M). Carbachol 154-163 gastrin releasing peptide Rattus norvegicus 61-64 1511949-4 1992 The secretion of insulin and PP in response to carbachol and GRP were dose-dependent in range of 10(-9) M to 10(-6) M. The responses of insulin and PP to carbachol (10(-7) M) were biphasic, and were abolished by atropine (10(-5) M). Carbachol 154-163 pancreatic polypeptide Rattus norvegicus 148-150 1511949-10 1992 The relative secretion of PP from the dorsal vs. the ventral portions of the rat pancreas following stimulation by carbachol and GRP (10(-7) M) was 19% and 22%, respectively, while that of insulin was 289% and 382%, respectively. Carbachol 115-124 pancreatic polypeptide Rattus norvegicus 26-28 1504815-4 1992 Compared with vehicle control animals, carbachol treated animals showed a significantly higher number of Fos-LI cells in pontine regions implicated in REM sleep generation, with longer REMc bouts associated with more Fos-LI cells than the short-duration bout. Carbachol 39-48 Fos proto-oncogene, AP-1 transcription factor subunit Felis catus 105-108 1504815-4 1992 Compared with vehicle control animals, carbachol treated animals showed a significantly higher number of Fos-LI cells in pontine regions implicated in REM sleep generation, with longer REMc bouts associated with more Fos-LI cells than the short-duration bout. Carbachol 39-48 Fos proto-oncogene, AP-1 transcription factor subunit Felis catus 217-220 1319016-6 1992 Acute (36-h) treatment with carbachol also caused an increase in VIP immunoreactivity (about 2-fold, and blocked by atropine) without an increase in VIP mRNA level. Carbachol 28-37 vasoactive intestinal peptide Homo sapiens 149-152 1575758-5 1992 Stimulation by caerulein, carbachol, or their combination caused an immediate and significant burst in both protein and GP2 outputs. Carbachol 26-35 glycoprotein 2 Rattus norvegicus 120-123 1590403-8 1992 The VIP-induced depolarization of Vbl was completely reversed by carbachol (0.1 mM; repolarization to -65 mV). Carbachol 65-74 VIP peptides Oryctolagus cuniculus 4-7 1315865-1 1992 8-Methoxy-4-[(2-isopropylphenyl)amino]-3-quinolinecarboxylate ethyl ester (AHR-9294) inhibited acid secretion stimulated by histamine, pentagastrin or carbachol in rats, and by histamine or feeding in dogs. Carbachol 151-160 aryl hydrocarbon receptor Rattus norvegicus 75-78 1529272-5 1992 2) PACAP significantly inhibited smooth-muscle contractions induced by acetylcholine or carbachol. Carbachol 88-97 adenylate cyclase activating polypeptide 1 Rattus norvegicus 3-8 1575736-0 1992 Carbachol stimulation of triacylglycerol lipase activity in pancreatic acinar cells. Carbachol 0-9 lipase C, hepatic type Homo sapiens 25-47 1314499-3 1992 Binding of 125I-labeled PYY was rapid (70% maximal within 10 min) and specific (not inhibited by secretin, vasoactive intestinal peptide, cholecystokinin, carbachol, prostaglandin E2, forskolin, or cholera toxin). Carbachol 155-164 peptide YY Homo sapiens 24-27 1591273-1 1992 Agents like carbachol and cholecystokinin (CCK), that activate chief cell phosphoinositidase C, thereby increasing cell calcium concentration, increase cAMP levels in cholera toxin-treated, but not control, gastric chief cells. Carbachol 12-21 cholecystokinin Homo sapiens 43-46 1591273-1 1992 Agents like carbachol and cholecystokinin (CCK), that activate chief cell phosphoinositidase C, thereby increasing cell calcium concentration, increase cAMP levels in cholera toxin-treated, but not control, gastric chief cells. Carbachol 12-21 phospholipase C beta 1 Homo sapiens 74-94 1373616-1 1992 In intact rat pancreatic acini, the phospholipase A2 inhibitor mepacrine did not affect basal amylase release but dose-dependently inhibited the carbachol (IC50 65 microM) and CCK-8 (IC50 210 microM)-stimulated amylase release. Carbachol 145-154 phospholipase A2 group IB Rattus norvegicus 36-52 1373616-3 1992 From these results we conclude that carbachol, CCK-8 and GTPrS probably activate a phospholipase A2 closely coupled to exocytosis. Carbachol 36-45 phospholipase A2 group IB Rattus norvegicus 83-99 1592746-4 1992 Airway responsiveness was measured 2 h post-PAF when sRL had returned to baseline and was expressed as the cumulative provocating dose of carbachol that increased sRL to 4 l.cmH2O.l-1.s (PD4). Carbachol 138-147 sarcalumenin Ovis aries 53-56 1581507-7 1992 Fourier transform infrared difference spectra calculated from spectra recorded in the presence and absence of carbamylcholine show small, reproducible bands which reflect changes in nAChR structure upon desensitization. Carbachol 110-125 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 182-187 1620239-6 1992 We conclude that carbachol promotes Ca2+ influx via a pertussis toxin-sensitive G protein and Ca2+ mobilization via a pertussis toxin-insensitive G-protein coupling to inositol phosphate generation; NPY stimulates Ca2+ mobilization via a pertussis toxin-sensitive G protein without apparent involvement of inositol phosphates. Carbachol 17-26 neuropeptide Y Homo sapiens 199-202 1347975-3 1992 However, they reversed vasoactive intestinal peptide (VIP)-induced inhibition (relaxation) of carbachol-stimulated contraction. Carbachol 94-103 VIP peptides Cavia porcellus 54-57 1318161-4 1992 The IC50 value of nifedipine for inhibition of ET-1 mediated contractions was 3.0 +/- 0.8 x 10(-8) M. ET-1 produced a marked prolonged homologous desensitization of its contractile response but did not affect the responses mediated by carbachol, histamine, serotonin, substance P, and PLA2. Carbachol 235-244 endothelin-1 Cavia porcellus 47-51 1318161-4 1992 The IC50 value of nifedipine for inhibition of ET-1 mediated contractions was 3.0 +/- 0.8 x 10(-8) M. ET-1 produced a marked prolonged homologous desensitization of its contractile response but did not affect the responses mediated by carbachol, histamine, serotonin, substance P, and PLA2. Carbachol 235-244 endothelin-1 Cavia porcellus 102-106 1312458-2 1992 NE and Carb markedly enhanced PI turnover (EC50: NE, 12 microM; Carb, 661 microM) as reflected by [3H]inositol monophosphate (IP1) accumulation in tissue slices prelabeled with [3H]myoinositol. Carbachol 7-11 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 126-129 1312458-6 1992 Preincubation of slices with various EEAs inhibited Carb-induced IP1 formation. Carbachol 52-56 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 65-68 1380376-5 1992 However, when a tonic contraction was induced with carbachol (1 microM) a prompt relaxation was induced by substance P (1- 100 nM) and by neurokinin A (1- 100 nM), with substance P being more potent. Carbachol 51-60 tachykinin 1 Mus musculus 107-118 1380376-5 1992 However, when a tonic contraction was induced with carbachol (1 microM) a prompt relaxation was induced by substance P (1- 100 nM) and by neurokinin A (1- 100 nM), with substance P being more potent. Carbachol 51-60 tachykinin 1 Mus musculus 138-150 1380376-5 1992 However, when a tonic contraction was induced with carbachol (1 microM) a prompt relaxation was induced by substance P (1- 100 nM) and by neurokinin A (1- 100 nM), with substance P being more potent. Carbachol 51-60 tachykinin 1 Mus musculus 169-180 1380376-11 1992 The selective NK1 tachykinin agonist, [Pro9]-SP sulphone (1 microM), exerted potent bronchodilator actions on carbachol-contracted mouse bronchial preparations. Carbachol 110-119 tachykinin 1 Mus musculus 14-17 1521562-1 1992 Using a perfused rat hindleg system, release of tissue-type plasminogen activator (t-PA) from endothelial cells could be induced by platelet-activating factor (PAF), bradykinin, substance P, thrombin, carbachol and A23187, while this release was inhibited by mepacrine and by nor-dihydroguaiaretic acid. Carbachol 201-210 plasminogen activator, tissue type Rattus norvegicus 48-81 1521562-1 1992 Using a perfused rat hindleg system, release of tissue-type plasminogen activator (t-PA) from endothelial cells could be induced by platelet-activating factor (PAF), bradykinin, substance P, thrombin, carbachol and A23187, while this release was inhibited by mepacrine and by nor-dihydroguaiaretic acid. Carbachol 201-210 plasminogen activator, tissue type Rattus norvegicus 83-87 1318210-1 1992 Three peptide components of atrial natriuretic factor (ANF) caused relaxation of carbachol-contracted guinea-pig isolated tracheal smooth muscle. Carbachol 81-90 natriuretic peptide A Rattus norvegicus 55-58 1372804-8 1992 Calmodulin antagonists inhibited amylase secretion induced by isoproterenol plus carbamylcholine or carbamylcholine alone, but not that induced by isoproterenol alone. Carbachol 81-96 calmodulin 1 Rattus norvegicus 0-10 1372804-8 1992 Calmodulin antagonists inhibited amylase secretion induced by isoproterenol plus carbamylcholine or carbamylcholine alone, but not that induced by isoproterenol alone. Carbachol 100-115 calmodulin 1 Rattus norvegicus 0-10 1313646-8 1992 When hormone-stimulated C kinase activity was enhanced with the diglyceride lipase inhibitor, RG 80267, BK- and carbachol-induced increases in [Ca2+]i were blunted 50%. Carbachol 112-121 pancreatic lipase related protein 1 Canis lupus familiaris 76-82 1545385-10 1992 In carbachol precontracted tissues, VIP elicited concentration-dependent relaxations that quickly desensitized. Carbachol 3-12 vasoactive intestinal peptide Sus scrofa 36-39 1592746-4 1992 Airway responsiveness was measured 2 h post-PAF when sRL had returned to baseline and was expressed as the cumulative provocating dose of carbachol that increased sRL to 4 l.cmH2O.l-1.s (PD4). Carbachol 138-147 sarcalumenin Ovis aries 163-166 1312940-2 1992 Low concentrations (100 nM) of cholecystokinin, neurotensin and vasoactive intestinal polypeptide (VIP), added in vitro, enhanced carbachol-stimulated phosphoinositide metabolism in cortical miniprisms from both young and senescent rats, while somatostatin was ineffective. Carbachol 130-139 neurotensin Rattus norvegicus 48-59 1353464-4 1992 PHI also decreased carbachol-stimulated antral gastrin release and simultaneously increased somatostatin release. Carbachol 19-28 gastrin Homo sapiens 47-54 1353464-7 1992 In addition, unlike its structural analogues, PHI inhibition of carbachol-stimulated gastrin release is not functionally linked to its stimulatory effects on somatostatin release. Carbachol 64-73 gastrin Homo sapiens 85-92 1312940-2 1992 Low concentrations (100 nM) of cholecystokinin, neurotensin and vasoactive intestinal polypeptide (VIP), added in vitro, enhanced carbachol-stimulated phosphoinositide metabolism in cortical miniprisms from both young and senescent rats, while somatostatin was ineffective. Carbachol 130-139 vasoactive intestinal peptide Rattus norvegicus 64-97 1312940-2 1992 Low concentrations (100 nM) of cholecystokinin, neurotensin and vasoactive intestinal polypeptide (VIP), added in vitro, enhanced carbachol-stimulated phosphoinositide metabolism in cortical miniprisms from both young and senescent rats, while somatostatin was ineffective. Carbachol 130-139 vasoactive intestinal peptide Rattus norvegicus 99-102 1312940-3 1992 Interestingly, the VIP receptor antagonist [d-parachloro-Phe6,Leu17[VIP shifted the dose-response curve for carbachol significantly to the right, indicating inhibition of phosphoinositide hydrolysis. Carbachol 108-117 vasoactive intestinal peptide Rattus norvegicus 19-22 1312940-3 1992 Interestingly, the VIP receptor antagonist [d-parachloro-Phe6,Leu17[VIP shifted the dose-response curve for carbachol significantly to the right, indicating inhibition of phosphoinositide hydrolysis. Carbachol 108-117 vasoactive intestinal peptide Rattus norvegicus 68-71 1310020-4 1992 Carbachol-mediated changes in neurite outgrowth, IP1 formation and [Ca2+]i displayed high sensitivity for pirenzepine but low sensitivity for AF-DX116. Carbachol 0-9 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 49-52 1731321-2 1992 Carbachol, a muscarinic receptor agonist, stimulated both calcium influx and inositol 1,4,5-trisphosphate (InsP3)-mediated intracellular calcium release in A9 fibroblast cells expressing a m3 muscarinic receptor clone. Carbachol 0-9 cholinergic receptor muscarinic 3 Homo sapiens 189-211 1636494-7 1992 The results of studies involving inhibitors of phospholipase A2 (PLA2) and phospholipase C (PLC) suggested that PLA2 is almost completely responsible for the Ca(2+)-dependent, carbachol-mediated AA liberation. Carbachol 176-185 phospholipase A2 group IB Rattus norvegicus 47-63 1636494-7 1992 The results of studies involving inhibitors of phospholipase A2 (PLA2) and phospholipase C (PLC) suggested that PLA2 is almost completely responsible for the Ca(2+)-dependent, carbachol-mediated AA liberation. Carbachol 176-185 phospholipase A2 group IB Rattus norvegicus 65-69 1636494-7 1992 The results of studies involving inhibitors of phospholipase A2 (PLA2) and phospholipase C (PLC) suggested that PLA2 is almost completely responsible for the Ca(2+)-dependent, carbachol-mediated AA liberation. Carbachol 176-185 phospholipase A2 group IB Rattus norvegicus 112-116 1609648-0 1992 Kininogen changes evoked in plasma by feeding, pseudo-alimentary, vagal and carbamylcholine stimulation of the rat. Carbachol 76-91 kininogen 2-like 1 Rattus norvegicus 0-9 1347631-2 1992 Activation of PPI hydrolysis by carbachol elicits a robust translocation of CaM from membranes into cytosol which was previously shown to be mimicked by the addition of the calcium ionophore ionomycin and the phorbol ester TPA28. Carbachol 32-41 calmodulin 3 Homo sapiens 76-79 1347631-9 1992 The CaM translocation with the combination of an agent that elevates cyclic AMP levels and a low dose of carbachol was not different from that observed with either agent alone. Carbachol 105-114 calmodulin 3 Homo sapiens 4-7 1347631-10 1992 UK 14,304, DPDPE and DAMGO potentiated carbachol-stimulated increases in cytosolic CaM. Carbachol 39-48 calmodulin 3 Homo sapiens 83-86 1620234-0 1992 Carbachol potentiates isoprenaline-induced mucin secretion by rat submandibular gland. Carbachol 0-9 solute carrier family 13 member 2 Rattus norvegicus 43-48 1583072-1 1992 Stimulation of chief cells with carbachol or cholecystokinin (CCK) results in the production of inositol trisphosphate (IP3) and diacylglycerol (DAG). Carbachol 32-41 cholecystokinin Homo sapiens 62-65 1364146-11 1992 Taken together with previous results showing that the activation of 5HT1C/5HT2 receptors promotes a reduction of the dipsogenic response evoked by water deprivation or by icv injection of angiotensin II or carbachol, the present data suggest that the central serotoninergic system plays a ubiquitous role in the modulation of water intake behavior. Carbachol 206-215 5-hydroxytryptamine receptor 2C Rattus norvegicus 68-73 1283979-2 1992 Calmodulin inhibitor (W-7, 100 microM) and Ca2+/CaM kinase II inhibitor (KN-62, 10 microM) reduced amylase secretion stimulated by cholecystokinin (CCK) or carbachol. Carbachol 156-165 calmodulin 1 Rattus norvegicus 0-10 1727052-7 1992 Moreover, carbachol inhibited the mitogenic effect of thyroid stimulating hormone (TSH) and of epidermal growth factor (EGF). Carbachol 10-19 epidermal growth factor Canis lupus familiaris 95-118 1727052-7 1992 Moreover, carbachol inhibited the mitogenic effect of thyroid stimulating hormone (TSH) and of epidermal growth factor (EGF). Carbachol 10-19 epidermal growth factor Canis lupus familiaris 120-123 1727052-9 1992 Nevertheless, carbachol enhanced the expression of the protooncogenes c-fos and c-myc mRNAs. Carbachol 14-23 MYC proto-oncogene, bHLH transcription factor Canis lupus familiaris 80-85 1596675-13 1992 At 10 microM, the highest concentration ?tsed, ET-1 produced similar levels of [3H]-InsP accumulation (7.03 +/- 0.55 fold above basal levels, t = 5) to that produced by a maximally effective concentration of carbachol (10 microM; 7.97 +/- 0.31 fold increase above basal levels, n = 4). Carbachol 208-217 endothelin 1 Rattus norvegicus 47-51 1596675-16 1992 The mean concentration of ET-1 producing 50% of the maximum contractile response to carbachol (EC50) was 31 nm (95% confidence limits, 20-49 nM, n = 12). Carbachol 84-93 endothelin 1 Rattus norvegicus 26-30 1285949-4 1992 Although carbachol or trihexyphenidyl alone was ineffective in inducing c-fos mRNA, the combination of levodopa and carbachol (> or = to 0.1 mg/kg) significantly suppressed the induction of c-fos mRNA as compared with levodopa given alone. Carbachol 116-125 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 193-198 1581016-2 1992 The effect of direct stimulation of the rostral TRN by the cholinergic agonist carbachol on the behaviour of freely moving rats was observed. Carbachol 79-88 transfer RNA asparagine 1 (anticodon GUU) Rattus norvegicus 48-51 1581016-3 1992 Unilateral injection of carbachol (0.2-3.2 micrograms/0.5 microliters) into the rostral TRN caused catalepsy which appeared rapidly and was short-lasting. Carbachol 24-33 transfer RNA asparagine 1 (anticodon GUU) Rattus norvegicus 88-91 19215537-7 1991 Inhibition of protein kinase C by sphingosine or by long-term treatment with phorbol esters, completely abolished the synthesis of CGA and CGB induced by carbamylcholine, bradykinin and prostaglandin E(2) but decreased only partially the stimulating effect of histamine. Carbachol 154-169 chromogranin A Bos taurus 131-134 1531470-1 1992 Both carbachol (10(-4)-10(-3) mol/l) and cholecystokinin octapeptide (CCK-8) (10(-8)-10(-6) mol/l) significantly stimulated the release of pepsinogen from rabbit gastric mucosa maintained in organ culture (213-216% and 143-261% of control, respectively, p less than 0.05-0.01). Carbachol 5-14 pepsin II-2/3 Oryctolagus cuniculus 139-149 1531470-5 1992 W-7 (10(-6)-10(-4) mol/l) and W-5 (10(-5) and 10(-4), or 10(-6) mol/l) reduced significantly the secretion of pepsinogen induced by carbachol (53-71% and 63-81% of control, respectively, p less than 0.05-0.01) and that by CCK-8 (49-67% and 66-76% of control, respectively, p less than 0.01) in the Ca2+ containing medium. Carbachol 132-141 pepsin II-2/3 Oryctolagus cuniculus 110-120 1531470-7 1992 These findings indicate that the calmodulin messenger branch that is activated by a rise of intracellular Ca2+ mobilised in cytosol from its intracellular, but not extracellular, source plays a critical role in pepsinogen secretion induced by carbachol and CCK-8. Carbachol 243-252 calmodulin Oryctolagus cuniculus 33-43 1531470-7 1992 These findings indicate that the calmodulin messenger branch that is activated by a rise of intracellular Ca2+ mobilised in cytosol from its intracellular, but not extracellular, source plays a critical role in pepsinogen secretion induced by carbachol and CCK-8. Carbachol 243-252 pepsin II-2/3 Oryctolagus cuniculus 211-221 1641133-10 1992 In contrast to the increased mRNA levels, nicotine and carbachol reduce the interleukin-1 alpha protein level measured in the rat adrenal gland: nicotine by approximately 30%, 60 min after injection, and carbachol by approximately 55%, 30 min after injection. Carbachol 55-64 interleukin 1 alpha Rattus norvegicus 76-95 1641133-10 1992 In contrast to the increased mRNA levels, nicotine and carbachol reduce the interleukin-1 alpha protein level measured in the rat adrenal gland: nicotine by approximately 30%, 60 min after injection, and carbachol by approximately 55%, 30 min after injection. Carbachol 204-213 interleukin 1 alpha Rattus norvegicus 76-95 1641133-11 1992 The interleukin-1 alpha protein level returns to control level 90 min after nicotine injection, and 120 min after carbachol injection. Carbachol 114-123 interleukin 1 alpha Rattus norvegicus 4-23 1940918-5 1991 Atropine, hexahydrosiladifenidol (HHSD), pirenzepine, and methoctramine inhibited the carbachol-evoked initial calcium transient with Ki values of 0.85 +/- 0.05, 8.3 +/- 1.6, 411 +/- 36, and 240 +/- 46 nM (mean +/- SEM, n = 3), respectively, and the acetylcholine-induced initial calcium transient with Ki values of 0.48 +/- 0.18, 13.5 +/- 8.5, 192 +/- 32, and 414 +/- 25 nM (mean +/- SEM of two experiments), respectively, results suggesting that an M3 muscarinic receptor was predominantly mediating these effects. Carbachol 86-95 cholinergic receptor muscarinic 3 Homo sapiens 451-473 1668612-4 1991 Carbachol decreased the Ca-inward current, and probably it prevented the calmodulin activation. Carbachol 0-9 calmodulin Oryctolagus cuniculus 73-83 1667338-5 1991 A direct relaxant effect of CGRP on smooth muscle tone of carbachol precontracted preparations was only observed in specimens of the guinea-pig. Carbachol 58-67 calcitonin related polypeptide alpha Homo sapiens 28-32 1478730-4 1992 Proenkephalin mRNA expression was stimulated by IL-1 beta in a time- and concentration-dependent manner, being maximal with 5 U/ml IL-1 beta at 4 h. Although the beta-adrenergic agonist isoproterenol was also active, interferon, glutamate, and carbachol were not. Carbachol 244-253 proenkephalin Rattus norvegicus 0-13 1478730-4 1992 Proenkephalin mRNA expression was stimulated by IL-1 beta in a time- and concentration-dependent manner, being maximal with 5 U/ml IL-1 beta at 4 h. Although the beta-adrenergic agonist isoproterenol was also active, interferon, glutamate, and carbachol were not. Carbachol 244-253 interleukin 1 beta Rattus norvegicus 48-57 1579044-3 1992 We now report that central administration of AII antagonists [either (Sar-1, Ile-8) AII or (Sar-1, Ala-8) AII] in rats prevents the majority (greater than 70%) of the pressor effects of intraventricular vasopressin or carbachol. Carbachol 218-227 angiotensinogen Rattus norvegicus 45-48 1641388-4 1992 Purified porcine PEC-60 was injected intravenously in mice at 1 or 8 nmol/kg alone or together with glucose (2.8 mmol/kg) or the cholinergic agonist carbachol (0.16 mumol/kg). Carbachol 149-158 serine peptidase inhibitor, Kazal type 4 Rattus norvegicus 17-23 1641388-5 1992 PEC-60 was found to inhibit glucose- and carbachol-induced insulin secretion (p less than 0.01 and p less than 0.05, respectively) at 8 nmol/kg, whereas at 1 nmol/kg, the peptide had no effect. Carbachol 41-50 serine peptidase inhibitor, Kazal type 4 Rattus norvegicus 0-6 1466092-2 1992 VIP caused a weak contraction and a small potentiation of carbachol- and acetylcholine-induced contractions. Carbachol 58-67 vasoactive intestinal peptide Rattus norvegicus 0-3 1722647-0 1991 Carbachol acts through protein kinase C to modulate cholecystokinin receptors on pancreatic acini. Carbachol 0-9 cholecystokinin Rattus norvegicus 52-67 1722647-4 1991 Preincubation with 0.1 mM carbachol for 60 min reduced the CCK octapeptide (CCK-8; 100 pM)-stimulated amylase release by 43 +/- 5%. Carbachol 26-35 cholecystokinin Rattus norvegicus 59-62 1722647-4 1991 Preincubation with 0.1 mM carbachol for 60 min reduced the CCK octapeptide (CCK-8; 100 pM)-stimulated amylase release by 43 +/- 5%. Carbachol 26-35 cholecystokinin Rattus norvegicus 76-79 1722647-7 1991 Preincubation of acini with 12-O-tetradecanoylphorbol 12,13-acetate (TPA, 1 microM), an activator of protein kinase C (PKC), decreased subsequent CCK-8-stimulated amylase release, and total binding of 125I-BH-CCK-8 to a similar extent as with pretreatment with CCh. Carbachol 261-264 cholecystokinin Rattus norvegicus 146-149 1722647-8 1991 The inhibitory effect of TPA or CCh on CCK-8-stimulated amylase release was reversed by simultaneous preincubation with H-7, an inhibitor of PKC. Carbachol 32-35 cholecystokinin Rattus norvegicus 39-42 1722647-10 1991 After CCh or TPA preincubation, CCK-8-stimulated production of [3H]inositol phosphates was inhibited by at least 49%. Carbachol 6-9 cholecystokinin Rattus norvegicus 32-35 1767816-7 1991 The pharmacological response of functional mAChRs, determined as accumulation of inositol phosphates induced by carbachol, is greater in the medium containing IGF-I and Ins than those cultured in 1% FBS. Carbachol 112-121 insulin like growth factor 1 Homo sapiens 159-164 1667295-0 1991 Chronic treatment with the angiotensin I converting enzyme inhibitor, perindopril, protects in vitro carbachol-induced vasorelaxation in a rat model of vascular calcium overload. Carbachol 101-110 angiotensin I converting enzyme Rattus norvegicus 27-58 19215537-4 1991 Incubation of cells with carbamylcholine, nigh K(+) or histamine, three potent stimulators of catecholamine secretion in chromaffin cells, increased the rate of CGA and CGB synthesis. Carbachol 25-40 chromogranin A Bos taurus 161-164 19215537-4 1991 Incubation of cells with carbamylcholine, nigh K(+) or histamine, three potent stimulators of catecholamine secretion in chromaffin cells, increased the rate of CGA and CGB synthesis. Carbachol 25-40 chromogranin B Bos taurus 169-172 19215537-7 1991 Inhibition of protein kinase C by sphingosine or by long-term treatment with phorbol esters, completely abolished the synthesis of CGA and CGB induced by carbamylcholine, bradykinin and prostaglandin E(2) but decreased only partially the stimulating effect of histamine. Carbachol 154-169 chromogranin B Bos taurus 139-142 1815136-3 1991 A series of cis- and trans-decahydroquinolines with substituents in the 2- and 5-position also exhibit structure-dependent inhibition of carbamylcholine-elicited sodium flux in PC12 cells and all of the decahydroquinolines inhibit binding of the noncompetitive blocking agent [3H]perhydrohistrionicotoxin to muscle-type nicotinic acetylcholine receptor-channels in membranes from Torpedo electroplax. Carbachol 137-152 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 320-352 1761164-3 1991 Maximal augmentation of the TRH-induced prolactin mRNA accumulation was obtained when cells were pretreated with 10(-5) M ACh for 24 h. The activation was mimicked by carbachol and oxotremorine and was blocked by the simultaneous presence of atropine. Carbachol 167-176 thyrotropin releasing hormone Rattus norvegicus 28-31 1761164-3 1991 Maximal augmentation of the TRH-induced prolactin mRNA accumulation was obtained when cells were pretreated with 10(-5) M ACh for 24 h. The activation was mimicked by carbachol and oxotremorine and was blocked by the simultaneous presence of atropine. Carbachol 167-176 prolactin Rattus norvegicus 40-49 1745608-0 1991 Effects of Ca2+ removal and of tetraethylammonium on membrane currents induced by carbachol in isolated cells from the rat parotid gland. Carbachol 82-91 carbonic anhydrase 2 Rattus norvegicus 11-14 1723045-2 1991 Neurokinin A and substance P produced concentration-dependent contractions which approached 80-90% of the maximal response to carbachol. Carbachol 126-135 tachykinin precursor 1 Homo sapiens 0-12 1723045-2 1991 Neurokinin A and substance P produced concentration-dependent contractions which approached 80-90% of the maximal response to carbachol. Carbachol 126-135 tachykinin precursor 1 Homo sapiens 17-28 1660519-4 1991 The specifically bound ANF could be displaced completely by unlabelled ANF(1-28) (biologically active circulating form of ANF), partially (approximately 50%) by the clearance receptor (ANF-R2) ligand, ANF(4-23), and insignificantly by angiotensin II, vasopressin, carbachol and progesterone. Carbachol 264-273 natriuretic peptide A Rattus norvegicus 23-26 1660519-4 1991 The specifically bound ANF could be displaced completely by unlabelled ANF(1-28) (biologically active circulating form of ANF), partially (approximately 50%) by the clearance receptor (ANF-R2) ligand, ANF(4-23), and insignificantly by angiotensin II, vasopressin, carbachol and progesterone. Carbachol 264-273 natriuretic peptide A Rattus norvegicus 71-74 1660519-4 1991 The specifically bound ANF could be displaced completely by unlabelled ANF(1-28) (biologically active circulating form of ANF), partially (approximately 50%) by the clearance receptor (ANF-R2) ligand, ANF(4-23), and insignificantly by angiotensin II, vasopressin, carbachol and progesterone. Carbachol 264-273 natriuretic peptide A Rattus norvegicus 71-74 1660519-4 1991 The specifically bound ANF could be displaced completely by unlabelled ANF(1-28) (biologically active circulating form of ANF), partially (approximately 50%) by the clearance receptor (ANF-R2) ligand, ANF(4-23), and insignificantly by angiotensin II, vasopressin, carbachol and progesterone. Carbachol 264-273 natriuretic peptide A Rattus norvegicus 71-74 1660519-4 1991 The specifically bound ANF could be displaced completely by unlabelled ANF(1-28) (biologically active circulating form of ANF), partially (approximately 50%) by the clearance receptor (ANF-R2) ligand, ANF(4-23), and insignificantly by angiotensin II, vasopressin, carbachol and progesterone. Carbachol 264-273 natriuretic peptide A Rattus norvegicus 71-74 1686865-3 1991 Here we report the time course of the appearance of the 3,5-dibromo-4-nitrosobenzene sulfonate (DBNBS) spin adduct and cyclic GMP formation following addition of carbamylcholine to suspensions of cultured mouse neuroblastoma cells (clone N1E-115). Carbachol 162-177 5'-nucleotidase, cytosolic II Mus musculus 126-129 1745608-5 1991 When pipettes contained no ATP, responses evoked by repeated applications of 10 microM carbachol (0.5-1 min) at 1.5-4 min intervals decreased only slowly after Ca2+ removal, outward currents being reduced to 90 +/- 6% and inward currents to 47 +/- 11% (n = 6) in 10 min. Carbachol 87-96 carbonic anhydrase 2 Rattus norvegicus 160-163 1745608-6 1991 On the other hand, when 5 mM ATP was included in the electrodes, Ca2+ removal abolished the carbachol responses in about 5 min (n = 4). Carbachol 92-101 carbonic anhydrase 2 Rattus norvegicus 65-68 1887927-10 1991 These results suggest that microinjection of NPY or carbachol into the posterior hypothalamic nucleus of conscious rats evokes an increase in MAP primarily as a result of sympathoexcitation and that NPY and carbachol selectively affect autonomic nervous system control of the cardiovascular system. Carbachol 52-61 neuropeptide Y Rattus norvegicus 199-202 1910075-14 1991 Ca(2+)-dependent and carbachol-mediated AA liberation occurs mainly as the result of PLA2 action. Carbachol 21-30 phospholipase A2 group IB Rattus norvegicus 85-89 1724672-0 1991 Effect of external Cd2+ and other divalent cations on carbachol-activated non-selective cation channels in guinea-pig ileum. Carbachol 54-63 T-cell surface antigen CD2 Cavia porcellus 19-22 1724672-7 1991 The inhibitory action of Cd2+ was associated neither with agonist concentration (CCh) nor with changes in reversal potential, and was voltage independent. Carbachol 81-84 T-cell surface antigen CD2 Cavia porcellus 25-28 1887927-10 1991 These results suggest that microinjection of NPY or carbachol into the posterior hypothalamic nucleus of conscious rats evokes an increase in MAP primarily as a result of sympathoexcitation and that NPY and carbachol selectively affect autonomic nervous system control of the cardiovascular system. Carbachol 207-216 neuropeptide Y Rattus norvegicus 45-48 1787877-4 1991 Cholinergic receptor agonists such as methacholine (10 microM), carbachol (10 microM), and oxotremorine (10 microM) inhibited the activity of serotonin N-acetyltransferase in the bovine pineal explants in culture, from a control value of 5.02 +/- 0.45 to 1.25 +/- 0.25, 1.30 +/- 0.15, and 1.22 +/- 0.20 pmol/mg protein/min, respectively. Carbachol 64-73 serotonin N-acetyltransferase Bos taurus 142-171 1679122-11 1991 Carbachol, a nonhydrolyzable cholinergic agonist, and 5-HT quickly and reversibly increased the amplitude of the LTT Ca2+ current. Carbachol 0-9 carbonic anhydrase 2 Rattus norvegicus 117-120 1877699-2 1991 The intracerebroventricular (icv) administration of carbachol (25 ng) resulted in marked transient increases in the plasma vasopressin concentration and mean arterial blood pressure and a transient reduction in heart rate. Carbachol 52-61 arginine vasopressin Rattus norvegicus 123-134 1720905-3 1991 In the presence of a submaximal dose of PACAP 38 (1 nM), amylase release stimulated by an agonist working via the elevation of intracellular cyclic AMP (VIP, dibutyryl cAMP) was additionally responded, but the amylase release stimulated by an agonist via the elevation of cytosolic free calcium (carbachol, cholecystokinin) was potentiated synergistically. Carbachol 296-305 vasoactive intestinal peptide Rattus norvegicus 153-156 1719434-12 1991 Both substance P and carbachol showed a concentration-related increase in accumulation of total inositol phosphates; though the maximal response to carbachol was considerably greater than that to any tachykinin (substance P, eledoisin, substance P methyl ester, senktide, neurokinin A, neurokinin B), or combination of two tachykinins (substance P and eledoisin, senktide and substance P methyl ester). Carbachol 21-30 tachykinin-3 Cavia porcellus 286-298 1719434-12 1991 Both substance P and carbachol showed a concentration-related increase in accumulation of total inositol phosphates; though the maximal response to carbachol was considerably greater than that to any tachykinin (substance P, eledoisin, substance P methyl ester, senktide, neurokinin A, neurokinin B), or combination of two tachykinins (substance P and eledoisin, senktide and substance P methyl ester). Carbachol 148-157 tachykinin-3 Cavia porcellus 286-298 1858855-2 1991 The frequency of oscillations increased in graded fashion with [CCh] between 25 nM (2.7 +/- 0.6 min-1) and 250 nM (11.8 +/- 1.4 min-1), whereas the amplitude of the spikes was independent of [CCh]. Carbachol 64-67 CD59 molecule (CD59 blood group) Homo sapiens 96-101 1712584-0 1991 Carbachol enhances forskolin-stimulated cyclic AMP accumulation via activation of calmodulin system in human neuroblastoma SH-SY5Y cells. Carbachol 0-9 calmodulin 1 Homo sapiens 82-92 1712584-6 1991 The enhancing response of carbachol was sensitive to trifluoperazine but insensitive to calphostin C. These results suggest that the mechanism for carbachol-induced cAMP levels may act, at least in part, through the activation of calmodulin system in SH-SY5Y cells. Carbachol 26-35 calmodulin 1 Homo sapiens 230-240 1712584-6 1991 The enhancing response of carbachol was sensitive to trifluoperazine but insensitive to calphostin C. These results suggest that the mechanism for carbachol-induced cAMP levels may act, at least in part, through the activation of calmodulin system in SH-SY5Y cells. Carbachol 147-156 calmodulin 1 Homo sapiens 230-240 1712584-7 1991 Hence we describe for the first time a synergistic interaction between calmodulin- and cAMP-dependent signal transduction pathway mediated by carbachol in neuron-derived cell line. Carbachol 142-151 calmodulin 1 Homo sapiens 71-81 1678851-8 1991 The administration of carbachol also stimulates rat adrenomedullary tyrosine hydroxylase gene transcription rate. Carbachol 22-31 tyrosine hydroxylase Rattus norvegicus 68-88 1649147-6 1991 ET1-stimulated IP3 production is dose dependent with an EC50 of 45 nM, this value is about 100- and 56-fold lower than those we reported for substance P and carbachol, respectively. Carbachol 157-166 endothelin-1 Oryctolagus cuniculus 0-3 1716708-4 1991 This carbachol-stimulated amylase release showed a biphasic curve, and its maximal response was observed at 10(-5) M. The release patterns of chymotrypsinogen and lipase were similar to that of amylase. Carbachol 5-14 lipase G, endothelial type Rattus norvegicus 163-169 1683694-3 1991 In contrast, the ability of isoprenaline to relax longitudinal smooth muscle precontracted with carbachol was significantly (p less than 0.02) reduced in the twenty-four month age group. Carbachol 96-105 neurogenin 3 Rattus norvegicus 44-49 1876601-4 1991 The purpose of this study was to examine the effects of IAPP and CGRP on glucose- and carbachol-stimulated release of insulin and pancreatic polypeptide (PP) from the isolated perfused rat pancreas. Carbachol 86-95 pancreatic polypeptide Rattus norvegicus 130-157 1716742-3 1991 NKA contracted large and small airways to a different extent (56% vs 92% of carbachol maximal contraction, respectively). Carbachol 76-85 tachykinin precursor 1 Homo sapiens 0-3 1716742-5 1991 SP had a lower contractile effect in large (26% carbachol maximum) and small airways (59%) with EC50 values higher than 0.5 microM. Carbachol 48-57 tachykinin precursor 1 Homo sapiens 0-2 1876601-6 1991 At the same concentration, however, IAPP significantly (p less than 0.05) inhibited carbachol-stimulated (10(-7) M) release of insulin by 30%, and CGRP significantly inhibited carbachol-stimulated release of insulin by 33% when compared with the control group. Carbachol 84-93 islet amyloid polypeptide Homo sapiens 36-40 1876601-6 1991 At the same concentration, however, IAPP significantly (p less than 0.05) inhibited carbachol-stimulated (10(-7) M) release of insulin by 30%, and CGRP significantly inhibited carbachol-stimulated release of insulin by 33% when compared with the control group. Carbachol 84-93 insulin Homo sapiens 127-134 1876601-6 1991 At the same concentration, however, IAPP significantly (p less than 0.05) inhibited carbachol-stimulated (10(-7) M) release of insulin by 30%, and CGRP significantly inhibited carbachol-stimulated release of insulin by 33% when compared with the control group. Carbachol 176-185 calcitonin related polypeptide alpha Homo sapiens 147-151 1876601-6 1991 At the same concentration, however, IAPP significantly (p less than 0.05) inhibited carbachol-stimulated (10(-7) M) release of insulin by 30%, and CGRP significantly inhibited carbachol-stimulated release of insulin by 33% when compared with the control group. Carbachol 176-185 insulin Homo sapiens 208-215 1876601-7 1991 IAPP also significantly decreased carbachol-stimulated release release of PP. Carbachol 34-43 islet amyloid polypeptide Homo sapiens 0-4 1876601-7 1991 IAPP also significantly decreased carbachol-stimulated release release of PP. Carbachol 34-43 pancreatic polypeptide Rattus norvegicus 2-4 1710445-2 1991 The bFGF-mediated calcium transient was not dependent on the presence of extracellular calcium, and was abolished by pretreatment of acini with carbachol. Carbachol 144-153 fibroblast growth factor 2 Rattus norvegicus 4-8 1768254-1 1991 Ornithine decarboxylase in a human parotid gland adenocarcinoma cell line was induced by both cholinergic (carbachol) and beta-adrenergic (isoproterenol) sialagogues. Carbachol 107-116 ornithine decarboxylase 1 Homo sapiens 0-23 2054187-5 1991 Muscarinic activation, either by repetitive stimulation of cholinergic fibers or by bath-applied carbachol, strongly increased intradendritic Ca2+ accumulation during directly evoked repetitive firing, in part by blocking a Ca(2+)-dependent K+ conductance. Carbachol 97-106 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 142-145 2013756-9 1991 These results demonstrate that phospholipase C hydrolysis of exogenous PI by rabbit cortical membranes may be stimulated by carbachol in a guanine nucleotide-dependent manner. Carbachol 124-133 LOC100009319 Oryctolagus cuniculus 31-46 2042942-6 1991 M1 muscarinic receptors exhibited both high (KH) and low (KL) affinities for the agonist carbachol. Carbachol 89-98 klotho Homo sapiens 58-60 1681055-10 1991 The negative inotropic responses of the A1-adenosine receptor agonist L-phenylisopropyladenosine (L-PIA) were also antagonized by 4-AP, but to a lesser extent than for carbachol. Carbachol 168-177 adenosine receptor A1 Cavia porcellus 40-61 1902229-4 1991 Using the stable acetylcholine analog carbachol to activate muscarinic receptors (mAChR) in a glial cell line (1321N1), we show that c-fos and c-jun mRNA levels are transiently increased, reaching a maximum at 30 min after agonist addition. Carbachol 38-47 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 133-138 1902229-4 1991 Using the stable acetylcholine analog carbachol to activate muscarinic receptors (mAChR) in a glial cell line (1321N1), we show that c-fos and c-jun mRNA levels are transiently increased, reaching a maximum at 30 min after agonist addition. Carbachol 38-47 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 143-148 1902229-5 1991 Experiments in which the actions of carbachol are blocked by adding atropine at various times demonstrate that only 1.5 min of agonist stimulation is needed to give maximal increases in c-fos or c-jun mRNA at 30 min. Carbachol 36-45 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 186-191 1902229-5 1991 Experiments in which the actions of carbachol are blocked by adding atropine at various times demonstrate that only 1.5 min of agonist stimulation is needed to give maximal increases in c-fos or c-jun mRNA at 30 min. Carbachol 36-45 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 195-200 1902229-7 1991 In cells in which protein kinase C has been down-regulated, carbachol no longer stimulates c-fos or c-jun expression, indicating a critical role for protein kinase C in these responses. Carbachol 60-69 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 91-96 1902229-7 1991 In cells in which protein kinase C has been down-regulated, carbachol no longer stimulates c-fos or c-jun expression, indicating a critical role for protein kinase C in these responses. Carbachol 60-69 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 100-105 1902229-9 1991 The strong enhancement of c-jun induction by carbachol in BAPTA-treated cells is due at least in part to mRNA stabilization. Carbachol 45-54 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 26-31 1708286-10 1991 The results suggest that the inhibitory effects of CCK and carbachol on net protein synthesis are due to their effects on intracellular calcium and PLA-mediated breakdown of phosphatidylcholine rather than protein kinase C activation. Carbachol 59-68 phospholipase A and acyltransferase 1 Rattus norvegicus 148-151 1848278-0 1991 Potential mechanisms involved in the negative coupling between serotonin 5-HT1A receptors and carbachol-stimulated phosphoinositide turnover in the rat hippocampus. Carbachol 94-103 5-hydroxytryptamine receptor 1A Rattus norvegicus 73-79 1848278-2 1991 In the present study, we have investigated further the pharmacological specificity of this negative control and attempted to elucidate the mechanism whereby 5-HT1A receptor activation inhibits the carbachol-stimulated phosphoinositide response in immature or adult rat hippocampal slices. Carbachol 197-206 5-hydroxytryptamine receptor 1A Rattus norvegicus 157-163 1848278-4 1991 The potency of the 5-HT1A receptor agonists investigated as inhibitors of the carbachol response was well correlated (r = 0.92) with their potency as inhibitors of the forskolin-stimulated adenylate cyclase in guinea pig hippocampal membranes. Carbachol 78-87 5-hydroxytryptamine receptor 1A Rattus norvegicus 19-25 1848278-9 1991 Moreover, the inhibitory effect of 8-OH-DPAT on the carbachol response was blocked by 10 microM quinacrine (a phospholipase A2 inhibitor) but not by BW 755C (100 microM), a cyclooxygenase and lipoxygenase inhibitor. Carbachol 52-61 phospholipase A2 group IB Rattus norvegicus 110-126 1848278-10 1991 These results collectively suggest that 5-HT1A receptor activation inhibits carbachol-stimulated phosphoinositide turnover by stimulating a phospholipase A2 coupled to 5-HT1A receptors, leading to arachidonic acid release. Carbachol 76-85 5-hydroxytryptamine receptor 1A Rattus norvegicus 40-46 1848278-10 1991 These results collectively suggest that 5-HT1A receptor activation inhibits carbachol-stimulated phosphoinositide turnover by stimulating a phospholipase A2 coupled to 5-HT1A receptors, leading to arachidonic acid release. Carbachol 76-85 phospholipase A2 group IB Rattus norvegicus 140-156 1848278-10 1991 These results collectively suggest that 5-HT1A receptor activation inhibits carbachol-stimulated phosphoinositide turnover by stimulating a phospholipase A2 coupled to 5-HT1A receptors, leading to arachidonic acid release. Carbachol 76-85 5-hydroxytryptamine receptor 1A Rattus norvegicus 168-174 1900518-5 1991 Likewise, IFN-gamma potentiated the action of the muscarinic agonist carbachol. Carbachol 69-78 interferon gamma Rattus norvegicus 10-19 2002334-2 1991 Chronic (3-72-h) agonist (nicotine or carbamylcholine) treatment of cells led to a complete (TE671) or nearly complete (PC12) loss of functional nAChR responses, which is referred to as "functional inactivation." Carbachol 38-53 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 145-150 2002334-8 1991 Recovery of TE671 cell nAChR function following treatment with carbamylcholine, nicotine, or d-TC occurred with half-times of 1-3 days whether cells were maintained in situ or harvested and replated after removal of ligand. Carbachol 63-78 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 23-28 2002344-3 1991 In addition, in the presence of ethanol (170-300 mM), CCh elevated levels of [3H]PEt [which is regarded as a specific indicator of phospholipase D (PLD) activity] by three- to sixfold. Carbachol 54-57 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 131-146 2002344-3 1991 In addition, in the presence of ethanol (170-300 mM), CCh elevated levels of [3H]PEt [which is regarded as a specific indicator of phospholipase D (PLD) activity] by three- to sixfold. Carbachol 54-57 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 148-151 1831422-0 1991 The release of atrial natriuretic factor induced by central carbachol injection may be mediated by muscarinic M1 receptors. Carbachol 60-69 natriuretic peptide A Rattus norvegicus 15-40 1831422-1 1991 We investigated the effect of selective muscarinic antagonists on atrial natriuretic factor (ANF) release induced by intracerebroventricular (i.c.v) injection of carbachol in the rat. Carbachol 162-171 natriuretic peptide A Rattus norvegicus 66-91 1831422-1 1991 We investigated the effect of selective muscarinic antagonists on atrial natriuretic factor (ANF) release induced by intracerebroventricular (i.c.v) injection of carbachol in the rat. Carbachol 162-171 natriuretic peptide A Rattus norvegicus 93-96 1831422-4 1991 4-Diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP), a rather selective M1 and M3 receptor antagonist, was the most potent inhibitor of carbachol-induced ANF release, its ID50 being 0.18 nmol/rat. Carbachol 141-150 natriuretic peptide A Rattus norvegicus 159-162 1831422-7 1991 The selective M2 receptor antagonist, methoctramine, on the other hand, was a very weak inhibitor of carbachol-induced ANF release. Carbachol 101-110 natriuretic peptide A Rattus norvegicus 119-122 2005087-5 1991 In contrast, the induction of TIS1 by NGF and TPA is slight and is only just detectable after stimulation by bFGF, but is strong for carbachol. Carbachol 133-142 nerve growth factor Rattus norvegicus 38-41 1848233-5 1991 The loss of response for both groups of agonists could be prevented if the incubation temperature was maintained at 37 degrees C, rather than at 10 degrees C. At the latter temperature carbachol pretreatment of SK-N-SH cells reduced the maximum release of inositol phosphates elicited by either carbachol or L-670,548 but not the agonist concentrations required for half-maximal stimulation. Carbachol 185-194 hedgehog acyltransferase Homo sapiens 211-215 1848233-5 1991 The loss of response for both groups of agonists could be prevented if the incubation temperature was maintained at 37 degrees C, rather than at 10 degrees C. At the latter temperature carbachol pretreatment of SK-N-SH cells reduced the maximum release of inositol phosphates elicited by either carbachol or L-670,548 but not the agonist concentrations required for half-maximal stimulation. Carbachol 295-304 hedgehog acyltransferase Homo sapiens 211-215 1704418-0 1991 Galanin reduces carbachol stimulation of phosphoinositide turnover in rat ventral hippocampus by lowering Ca2+ influx through voltage-sensitive Ca2+ channels. Carbachol 16-25 galanin and GMAP prepropeptide Rattus norvegicus 0-7 1364828-2 1991 The effects of vasoactive intestinal peptide (VIP) on contractile responses to carbachol (CCh), KCl and caffeine of the circular smooth muscle in rat stomach were examined by the isometric tension recording method and by measurement of the intracellular Ca level, [Ca]i, with fura 2. Carbachol 79-88 vasoactive intestinal peptide Rattus norvegicus 15-44 1364828-2 1991 The effects of vasoactive intestinal peptide (VIP) on contractile responses to carbachol (CCh), KCl and caffeine of the circular smooth muscle in rat stomach were examined by the isometric tension recording method and by measurement of the intracellular Ca level, [Ca]i, with fura 2. Carbachol 79-88 vasoactive intestinal peptide Rattus norvegicus 46-49 1364828-2 1991 The effects of vasoactive intestinal peptide (VIP) on contractile responses to carbachol (CCh), KCl and caffeine of the circular smooth muscle in rat stomach were examined by the isometric tension recording method and by measurement of the intracellular Ca level, [Ca]i, with fura 2. Carbachol 90-93 vasoactive intestinal peptide Rattus norvegicus 15-44 1364828-2 1991 The effects of vasoactive intestinal peptide (VIP) on contractile responses to carbachol (CCh), KCl and caffeine of the circular smooth muscle in rat stomach were examined by the isometric tension recording method and by measurement of the intracellular Ca level, [Ca]i, with fura 2. Carbachol 90-93 vasoactive intestinal peptide Rattus norvegicus 46-49 1364828-7 1991 VIP dose-dependently inhibited the contraction induced by 1 microM CCh, but not those caused by 40 mM KCl or 3 mM caffeine. Carbachol 67-70 vasoactive intestinal peptide Rattus norvegicus 0-3 1364828-9 1991 In Ca-free solution containing 2 mM EGTA, VIP inhibited the phasic contraction induced by 100 microM CCh, but not that induced by 30 mM caffeine. Carbachol 101-104 vasoactive intestinal peptide Rattus norvegicus 42-45 1364828-15 1991 It is suggested that the inhibitory effects of VIP on CCh-induced contractions are due to the inhibition of the processes of signal transduction from muscarinic receptors to voltage-dependent Ca channels and to intracellular Ca stores. Carbachol 54-57 vasoactive intestinal peptide Rattus norvegicus 47-50 1886889-5 1991 Furthermore, GLP-1(7-36) amide showed additive stimulatory influence with glucose (2.8 mmol/kg), the cholinergic agonist carbachol (0.16 mumol/kg), and the C-terminal octapeptide of cholecystokinin (CCK-8, 5.3 nmol/kg), when injected at 8 or 32 nmol/kg. Carbachol 121-130 glucagon Mus musculus 13-18 1886889-7 1991 Moreover, the glucagon secretory responses to carbachol and CCK-8 were inhibited by GLP-1(7-36) amide but were unaffected by the entire GLP-1. Carbachol 46-55 glucagon Mus musculus 84-89 1704418-1 1991 The 29-amino-acid peptide galanin (GAL) caused concentration-dependent inhibition of the accumulation of 3H-inositol phosphates (3H-InsPs) induced by the muscarinic agonist carbachol (CARB; 10(-3)-10(-5) M) in the presence of 5 mM lithium, specifically in tissue miniprisms from rat ventral hippocampus. Carbachol 173-182 galanin and GMAP prepropeptide Rattus norvegicus 26-33 1704418-1 1991 The 29-amino-acid peptide galanin (GAL) caused concentration-dependent inhibition of the accumulation of 3H-inositol phosphates (3H-InsPs) induced by the muscarinic agonist carbachol (CARB; 10(-3)-10(-5) M) in the presence of 5 mM lithium, specifically in tissue miniprisms from rat ventral hippocampus. Carbachol 173-182 galanin and GMAP prepropeptide Rattus norvegicus 35-38 1704418-1 1991 The 29-amino-acid peptide galanin (GAL) caused concentration-dependent inhibition of the accumulation of 3H-inositol phosphates (3H-InsPs) induced by the muscarinic agonist carbachol (CARB; 10(-3)-10(-5) M) in the presence of 5 mM lithium, specifically in tissue miniprisms from rat ventral hippocampus. Carbachol 184-188 galanin and GMAP prepropeptide Rattus norvegicus 26-33 1704418-1 1991 The 29-amino-acid peptide galanin (GAL) caused concentration-dependent inhibition of the accumulation of 3H-inositol phosphates (3H-InsPs) induced by the muscarinic agonist carbachol (CARB; 10(-3)-10(-5) M) in the presence of 5 mM lithium, specifically in tissue miniprisms from rat ventral hippocampus. Carbachol 184-188 galanin and GMAP prepropeptide Rattus norvegicus 35-38 1704418-2 1991 The inhibitory effect of GAL involved the mono-, bis-, tris-, and tetrakisphosphates formed during activation for 2 min of phospholipase C by CARB (1 mM) in the absence of lithium. Carbachol 142-146 galanin and GMAP prepropeptide Rattus norvegicus 25-28 1704418-6 1991 Reduction of the extracellular Ca2+ concentration from 1.3 mM to 0.49 microM abolished the GAL inhibition of CARB-stimulated PI hydrolysis. Carbachol 109-113 galanin and GMAP prepropeptide Rattus norvegicus 91-94 1716879-1 1991 Both beta-adrenergic (isoproterenol) and cholinergic (carbachol) sialagogues increase amylase secretion, ornithine decarboxylase activity and DNA synthesis in murine parotid gland in vivo and in vitro. Carbachol 54-63 ornithine decarboxylase, structural 1 Mus musculus 105-128 2055244-0 1991 Modulation of Na+, Cl- and HCO3- transport by carbachol in pig distal jejunum. Carbachol 46-55 HCO3 Sus scrofa 27-31 2055244-9 1991 The effects of carbachol on HCO3- transport were examined by pH-stat titration. Carbachol 15-24 HCO3 Sus scrofa 28-32 2055244-12 1991 Furthermore, the ability of carbachol to inhibit spontaneous Na+ absorption is dependent upon extracellular HCO3-. Carbachol 28-37 HCO3 Sus scrofa 108-112 2055247-5 1991 Maximal NmU-induced contractions were 10% of the maximal contraction induced by carbachol and ranged between those of the bradykinin and angiotensin II. Carbachol 80-89 neuromedin U Rattus norvegicus 8-11 1987779-11 1991 Pretreatment of cells with neurotensin, carbachol, or ATP desensitized subsequent neurotensin-stimulated efflux by 82, 57, and 63%, respectively, confirming our previous results which indicated homologous and heterologous desensitization of the neurotensin receptor-signal transduction pathway. Carbachol 40-49 neurotensin Homo sapiens 82-93 1987779-11 1991 Pretreatment of cells with neurotensin, carbachol, or ATP desensitized subsequent neurotensin-stimulated efflux by 82, 57, and 63%, respectively, confirming our previous results which indicated homologous and heterologous desensitization of the neurotensin receptor-signal transduction pathway. Carbachol 40-49 neurotensin Homo sapiens 82-93 1797275-2 1991 Since stimulation of adrenergic or cholinergic receptors has no effect on glomerular filtration rate, the antidiuresis and significant delay in urinary flow observed after lateral hypothalamic stimulation with carbachol (CCh) (0.036 +/- 0.005 to 0.019 +/- 0.003 microliters min-1 100 g body weight-1) and noradrenaline (Nad) (0.024 +/- 0.005 to 0.025 +/- 0.004 microliters min-1 100 g body weight-1) are secondary to an increase in distal tubular fluid reabsorption (DFR). Carbachol 210-219 Body weight QTL 17 Rattus norvegicus 291-299 1797275-2 1991 Since stimulation of adrenergic or cholinergic receptors has no effect on glomerular filtration rate, the antidiuresis and significant delay in urinary flow observed after lateral hypothalamic stimulation with carbachol (CCh) (0.036 +/- 0.005 to 0.019 +/- 0.003 microliters min-1 100 g body weight-1) and noradrenaline (Nad) (0.024 +/- 0.005 to 0.025 +/- 0.004 microliters min-1 100 g body weight-1) are secondary to an increase in distal tubular fluid reabsorption (DFR). Carbachol 210-219 Body weight QTL 17 Rattus norvegicus 390-398 2003581-1 1991 The effects of carbamylcholine (carbachol) on intracellular Ca2+ concentration ([Ca2+]c) of T84 cells were examined using the fluorescent Ca2+ indicator fura-2 and microfluorometric techniques. Carbachol 15-30 carbonic anhydrase 2 Homo sapiens 60-63 2003581-1 1991 The effects of carbamylcholine (carbachol) on intracellular Ca2+ concentration ([Ca2+]c) of T84 cells were examined using the fluorescent Ca2+ indicator fura-2 and microfluorometric techniques. Carbachol 32-41 carbonic anhydrase 2 Homo sapiens 60-63 2003581-1 1991 The effects of carbamylcholine (carbachol) on intracellular Ca2+ concentration ([Ca2+]c) of T84 cells were examined using the fluorescent Ca2+ indicator fura-2 and microfluorometric techniques. Carbachol 32-41 carbonic anhydrase 2 Homo sapiens 81-84 2003581-1 1991 The effects of carbamylcholine (carbachol) on intracellular Ca2+ concentration ([Ca2+]c) of T84 cells were examined using the fluorescent Ca2+ indicator fura-2 and microfluorometric techniques. Carbachol 32-41 carbonic anhydrase 2 Homo sapiens 81-84 2003581-2 1991 In single isolated cells, carbachol (100 microM) caused a rapid increase in [Ca2+]c of 184 +/- 15 nM (SE, n = 44) from a resting value of 56 +/- 7 nM. Carbachol 26-35 carbonic anhydrase 2 Homo sapiens 77-80 2003581-13 1991 These results show that a rise in [Ca2+]c and oscillations is likely to underlie the membrane K+ current responses to carbachol in T84 cells. Carbachol 118-127 carbonic anhydrase 2 Homo sapiens 35-38 1902458-5 1991 After air-sham exposure, carbachol and histamine increased mean sRL to 370 +/- 40 (SE) and 309 +/- 65% of baseline, respectively. Carbachol 25-34 sarcalumenin Ovis aries 64-67 1902458-7 1991 Prior 30-min exposure to hyperoxia prevented the hypoxia-induced enhancement of bronchial reactivity to carbachol (sRL = 416 +/- 66% of baseline) and histamine (sRL = 292 +/- 41% of baseline) without affecting the airway responsiveness to these agents after air. Carbachol 104-113 sarcalumenin Ovis aries 115-118 1999473-1 1991 Carbachol, through a muscarinic receptor, thyrotropin-releasing hormone (TRH), prostaglandin F2 alpha (PGF2 alpha), bradykinin, and adenosine triphosphate (ATP) increased the apparent [Ca2+]i (intracellular free Ca2(+)-concentration) of dog thyrocytes in primary culture. Carbachol 0-9 TRH Canis lupus familiaris 42-71 1999473-1 1991 Carbachol, through a muscarinic receptor, thyrotropin-releasing hormone (TRH), prostaglandin F2 alpha (PGF2 alpha), bradykinin, and adenosine triphosphate (ATP) increased the apparent [Ca2+]i (intracellular free Ca2(+)-concentration) of dog thyrocytes in primary culture. Carbachol 0-9 TRH Canis lupus familiaris 73-76 1999473-1 1991 Carbachol, through a muscarinic receptor, thyrotropin-releasing hormone (TRH), prostaglandin F2 alpha (PGF2 alpha), bradykinin, and adenosine triphosphate (ATP) increased the apparent [Ca2+]i (intracellular free Ca2(+)-concentration) of dog thyrocytes in primary culture. Carbachol 0-9 kininogen 1 Canis lupus familiaris 116-126 1675835-3 1991 Muscarinic receptors (carbachol-stimulated ileum) were only influenced (competitively) at 10(-7) M or above, suggesting that the non-competitive effects described above could be due to a specific reaction with the histamine H1 receptor. Carbachol 22-31 histamine H1 receptor Cavia porcellus 214-235 12106186-1 1991 Single electrode current clamp and voltage clamp recordings were employed to study the effects of noradrenergic agonists and a cholinergic agonist (carbachol, Cch) on the resting membrane potential of CA3 neurons in guinea pig hippocampal slices. Carbachol 148-157 carbonic anhydrase 3 Cavia porcellus 201-204 1716879-4 1991 Isoproterenol-dependent ornithine decarboxylase induction and phosphorylation of the proteins were selectively suppressed by monensin but not by polymyxin B, whereas carbachol-dependent ornithine decarboxylase induction and protein phosphorylation were inhibited by polymyxin B but not by monensin. Carbachol 166-175 ornithine decarboxylase 1 Rattus norvegicus 186-209 1716879-7 1991 Propranolol and atropine, antagonists of isoproterenol and carbachol, respectively, completely inhibited not only amylase secretion and ornithine decarboxylase induction but also protein phosphorylation stimulated by the corresponding agonists. Carbachol 59-68 ornithine decarboxylase 1 Rattus norvegicus 136-159 1988650-0 1991 Protein kinase C translocation in rat stomach fundus: effects of serotonin, carbamylcholine and phorbol dibutyrate. Carbachol 76-91 protein kinase C, gamma Rattus norvegicus 0-16 2050284-6 1991 We found that Ca reversal was observed in the smooth muscle of rat thoracic aorta contracted by norepinephrine or by a phorbol ester, TPA, guinea pig tracheal smooth muscle contracted to carbachol, fundic smooth muscle of rat stomach to carbachol, corporal and antral smooth muscle of guinea pig stomach to carbachol and rat vas deferens to norepinephrine. Carbachol 187-196 plasminogen activator, tissue type Rattus norvegicus 134-137 1988650-8 1991 However, PDBu (10 nM-1 microM) and carbamylcholine (0.1-10 microM) significantly increased membrane-bound PKC activity. Carbachol 35-50 protein kinase C, gamma Rattus norvegicus 106-109 1988650-9 1991 These results: 1) demonstrate that translocation of PKC occurred in rat stomach fundus in response to some, but not all, contractile agonists; 2) are consistent with the possibility that contraction of rat stomach fundus by carbamylcholine and PDBu may be related to increased membrane-bound PKC activity; and 3) indicate that serotonin-induced contraction, although potently blocked by staurosporine, did not result from PKC translocation in the rat stomach fundus. Carbachol 224-239 protein kinase C, gamma Rattus norvegicus 52-55 1988650-9 1991 These results: 1) demonstrate that translocation of PKC occurred in rat stomach fundus in response to some, but not all, contractile agonists; 2) are consistent with the possibility that contraction of rat stomach fundus by carbamylcholine and PDBu may be related to increased membrane-bound PKC activity; and 3) indicate that serotonin-induced contraction, although potently blocked by staurosporine, did not result from PKC translocation in the rat stomach fundus. Carbachol 224-239 protein kinase C, gamma Rattus norvegicus 292-295 1988650-9 1991 These results: 1) demonstrate that translocation of PKC occurred in rat stomach fundus in response to some, but not all, contractile agonists; 2) are consistent with the possibility that contraction of rat stomach fundus by carbamylcholine and PDBu may be related to increased membrane-bound PKC activity; and 3) indicate that serotonin-induced contraction, although potently blocked by staurosporine, did not result from PKC translocation in the rat stomach fundus. Carbachol 224-239 protein kinase C, gamma Rattus norvegicus 292-295 1825686-3 1991 Carbachol stimulated the rate of inositol phospholipid breakdown in IMR-32 and SK-N-MC human neuroblastoma cells with an EC50 value of about 50 microM in both cases. Carbachol 0-9 hedgehog acyltransferase Homo sapiens 79-83 1825686-4 1991 Pirenzepine inhibited the carbachol (100 microM)-stimulated inositol phospholipid breakdown in both cells with Hill slopes of unity and IC50 values of 15 nM (IMR-32) and 12 nM (SK-N-MC). Carbachol 26-35 hedgehog acyltransferase Homo sapiens 177-181 1646361-6 1991 Bovine adrenal glands stimulated with 100 microM carbachol released 0.47 +/- 0.12 nmol/gland of Ap4A and 1.11 +/- 0.26 nmol/gland of Ap5A. Carbachol 49-58 Jun proto-oncogene, AP-1 transcription factor subunit Bos taurus 96-106 1825686-5 1991 The 5-HT1A receptor agonist 8-OH-DPAT competitively inhibited carbachol-stimulated inositol phospholipid breakdown with pA2 values of 5.78 (IMR-32) and 5.61 (SK-N-MC). Carbachol 62-71 5-hydroxytryptamine receptor 1A Homo sapiens 4-19 1825686-5 1991 The 5-HT1A receptor agonist 8-OH-DPAT competitively inhibited carbachol-stimulated inositol phospholipid breakdown with pA2 values of 5.78 (IMR-32) and 5.61 (SK-N-MC). Carbachol 62-71 hedgehog acyltransferase Homo sapiens 158-162 1646447-4 1991 In contrast to the results obtained with the binding experiments using antagonists, the study of the cellular cyclic nucleotide response upon carbachol stimulation suggested the presence of both the M1 and M2 subtypes as there was an increase in cyclic GMP concentration while at the same time, the prostaglandin-stimulated synthesis of cyclic AMP was inhibited. Carbachol 142-151 5'-nucleotidase, cytosolic II Mus musculus 253-256 2176212-6 1990 Treatment of 1321N1 cells with carbachol results in increases in radiolabeled choline, phosphatidic acid (PA) and phosphatidylethanol (PEt), metabolites that are products of phospholipase D (PLD) action on PC. Carbachol 31-40 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 174-189 2176212-6 1990 Treatment of 1321N1 cells with carbachol results in increases in radiolabeled choline, phosphatidic acid (PA) and phosphatidylethanol (PEt), metabolites that are products of phospholipase D (PLD) action on PC. Carbachol 31-40 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 191-194 2176212-9 1990 It appears that most of the DG is formed through the action of PLD, since propranolol (which inhibits the conversion of PA to DG) and down-regulation of protein kinase C (which prevents activation of PLD by carbachol) both markedly inhibit DG production. Carbachol 207-216 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 63-66 2176212-9 1990 It appears that most of the DG is formed through the action of PLD, since propranolol (which inhibits the conversion of PA to DG) and down-regulation of protein kinase C (which prevents activation of PLD by carbachol) both markedly inhibit DG production. Carbachol 207-216 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 200-203 2176212-10 1990 Using a protocol in which cells are stimulated with carbachol for only one minute (a period during which PLD and PA formation are maximally activated), we show that DG mass continues to increase following removal of agonist. Carbachol 52-61 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 105-108 2176213-6 1990 Surprisingly, however, carbachol is not a mitogen for 39M1-81 cells, and even if tested in association with insulin or fibroblast growth factor, its effects on cell proliferation remained weak when compared with thrombin. Carbachol 23-32 insulin Cricetulus griseus 108-115 2260641-0 1990 VIP and forskolin enhance carbachol-induced K+ efflux from rat salivary gland fragments by a Ca2(+)-sensitive mechanism. Carbachol 26-35 vasoactive intestinal peptide Rattus norvegicus 0-3 2293110-2 1990 Encouraged by the finding that microinjections of the cholinergic agonist carbachol into the medial pontine reticular formation (mPRF) of the cat produced respiratory changes paralleling those observed during rapid eye movement (REM) sleep (Neurosci. Carbachol 74-83 Spi-C transcription factor (Spi-1/PU.1 related) Mus musculus 129-133 2260641-1 1990 The effect of vasoactive intestinal peptide (VIP) and forskolin on carbachol-induced K+ release from superfused rat submandibular and parotid gland fragments was examined using a K(+)-sensitive electrode. Carbachol 67-76 vasoactive intestinal peptide Rattus norvegicus 14-43 2260641-1 1990 The effect of vasoactive intestinal peptide (VIP) and forskolin on carbachol-induced K+ release from superfused rat submandibular and parotid gland fragments was examined using a K(+)-sensitive electrode. Carbachol 67-76 vasoactive intestinal peptide Rattus norvegicus 45-48 2260641-4 1990 VIP (1 microM) lacked effect on K+ efflux on its own but increased the carbachol (1 microM)-evoked K+ release. Carbachol 71-80 vasoactive intestinal peptide Rattus norvegicus 0-3 2260641-6 1990 Omission of Ca2+ from the medium totally abolished the augmenting effect of VIP and forskolin on carbachol-evoked K+ efflux. Carbachol 97-106 vasoactive intestinal peptide Rattus norvegicus 76-79 2260641-10 1990 It is concluded that VIP, by increasing the intracellular levels of cAMP in the glandular cell, potentiates carbachol-evoked Ca2(+)-dependent K+ efflux. Carbachol 108-117 vasoactive intestinal peptide Rattus norvegicus 21-24 2124620-5 1990 Carbachol stimulated the release of [3H]lysophosphatidylcholine from [3H]choline prelabeled cells, suggesting that phospholipase A2 was involved in the release of arachidonic acid. Carbachol 0-9 phospholipase A2 Cricetulus griseus 115-131 1707744-1 1990 The outward current evoked in CA1 neurons by brief anoxia is strongly voltage dependent and is abolished by an atropine-sensitive action of carbachol (and also when recording with a GTP gamma S-containing microelectrode). Carbachol 140-149 carbonic anhydrase 1 Homo sapiens 30-33 1707744-4 1990 It is suggested that an early release of Ca2+ from a dantrolene (and perhaps GTP)-sensitive internal store activates Ca-sensitive Cl channels, as well as carbachol-sensitive (mainly M-type?) Carbachol 154-163 carbonic anhydrase 2 Homo sapiens 41-44 2174803-7 1990 In the present report we demonstrate that the acetylcholine analog carbachol enhanced toxin-stimulated cyclic GMP accumulation in intact T84 cells by 50-100% and that this effect was blocked by 10 microM atropine and 10 microM sphingosine. Carbachol 67-76 5'-nucleotidase, cytosolic II Homo sapiens 110-113 2174803-0 1990 Carbachol mimics phorbol esters in its ability to enhance cyclic GMP production by STa, the heat-stable toxin of Escherichia coli. Carbachol 0-9 5'-nucleotidase, cytosolic II Homo sapiens 65-68 2174803-9 1990 Carbachol, which elevates intracellular calcium in these cells, may act through protein kinase C to enhance cyclic GMP production. Carbachol 0-9 5'-nucleotidase, cytosolic II Homo sapiens 115-118 1981603-8 1990 Sympathomimetic beta adrenergic agonists relaxed carbachol-precontracted tracheas with the following order of potency: isoprenaline (beta 1 and beta 2 adrenoceptor agonist) greater than salbutamol (beta 2 adrenoceptor agonist) greater than prenalterol (beta 1 adrenoceptor agonist). Carbachol 49-58 adrenergic receptor, beta 1 Mus musculus 133-163 1981603-8 1990 Sympathomimetic beta adrenergic agonists relaxed carbachol-precontracted tracheas with the following order of potency: isoprenaline (beta 1 and beta 2 adrenoceptor agonist) greater than salbutamol (beta 2 adrenoceptor agonist) greater than prenalterol (beta 1 adrenoceptor agonist). Carbachol 49-58 adrenergic receptor, beta 2 Mus musculus 144-163 1981603-8 1990 Sympathomimetic beta adrenergic agonists relaxed carbachol-precontracted tracheas with the following order of potency: isoprenaline (beta 1 and beta 2 adrenoceptor agonist) greater than salbutamol (beta 2 adrenoceptor agonist) greater than prenalterol (beta 1 adrenoceptor agonist). Carbachol 49-58 adrenergic receptor, beta 1 Mus musculus 253-272 2074719-4 1990 Output of both pH and HCO3- in these tissues was significantly increased by intravenous administration of prostaglandin (PGE2), 16, 16-dimethyl PGE2, carbachol, and YM-14673 (a thyrotropin-releasing hormone (TRH) analog), whereas the PD responded to these agents by a significant rise in the duodenum and decrease in the stomach. Carbachol 150-159 thyrotropin releasing hormone Rattus norvegicus 177-206 1702343-0 1990 Spinal cord substance P mediates carbachol-induced cardiovascular responses from the rostral ventrolateral medulla. Carbachol 33-42 tachykinin precursor 1 Homo sapiens 12-23 1701910-6 1990 An initial stimulation of cells with a high dose of CCK eliminated the Ca2+i response to further stimulation with CCK, carbachol, bombesin and SP, whereas cells subjected to initial stimulation with SP responded to subsequent exposure to CCK with prolonged elevation of Ca2+i. Carbachol 119-128 cholecystokinin Homo sapiens 52-55 2257432-23 1990 A 1 microM concentration of either fragment was equivalent in effect to 30nm NPY upon basal current, but NPY at this concentration significantly reduced both CCh- and SP-induced secretion. Carbachol 158-161 neuropeptide Y Rattus norvegicus 105-108 1700270-3 1990 The effects of carbachol on forskolin-stimulated cAMP levels were further augmented approximately 10-fold in the presence of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (MIX) but not in the presence of "low Km" cGMP-inhibited phosphodiesterase inhibitor milrinone. Carbachol 15-24 phosphodiesterase 3A, cGMP inhibited Mus musculus 227-259 1700270-8 1990 Cyclic GMP levels were also enhanced by carbachol plus isoproterenol. Carbachol 40-49 5'-nucleotidase, cytosolic II Mus musculus 7-10 2226784-2 1990 Carbachol induced an increase in intracellular Ca2+ and stimulated gastrin release in a dose-dependent manner over the range 10(-5)-10(-3) M. These effects were completely abolished by atropine, suggesting the implication of muscarinic cholinergic receptors. Carbachol 0-9 gastrin Rattus norvegicus 67-74 2226784-7 1990 These results suggest the M3 muscarinic receptors may be involved in the carbachol-induced gastrin release from B6 RIN cells. Carbachol 73-82 gastrin Rattus norvegicus 91-98 2167677-2 1990 After a 45-min incubation at 37 degrees of N1E-115 cells either in monolayer or in suspension, with the muscarinic agonist carbachol (1 mM), the receptor-mediated cyclic GMP response to carbachol was nearly completely lost. Carbachol 123-132 5'-nucleotidase, cytosolic II Mus musculus 170-173 2167677-2 1990 After a 45-min incubation at 37 degrees of N1E-115 cells either in monolayer or in suspension, with the muscarinic agonist carbachol (1 mM), the receptor-mediated cyclic GMP response to carbachol was nearly completely lost. Carbachol 186-195 5'-nucleotidase, cytosolic II Mus musculus 170-173 2282459-5 1990 In contrast, CGRP sometimes reduced spontaneous or carbamylcholine-induced tone of lung parenchymal strips. Carbachol 51-66 calcitonin-related polypeptide alpha Rattus norvegicus 13-17 2282459-7 1990 CGRP produced a significant rightward shift of the log concentration-response curves to carbamylcholine and 5-hydroxytryptamine (5-HT) in the main bronchus. Carbachol 88-103 calcitonin-related polypeptide alpha Rattus norvegicus 0-4 2282459-11 1990 In all three airway preparations, CGRP caused concentration-dependent inhibition of responses elicited by challenges with 10(-7) M carbamylcholine or 5 x 10(-7) M 5-HT. Carbachol 131-146 calcitonin-related polypeptide alpha Rattus norvegicus 34-38 2381568-9 1990 carbachol injection were reduced 1-2 days after AV3V lesion (delta MAP = 6 +/- 2 mmHg, water ingestion = 0.3 +/- 0.3 ml/h and Na+ excretion = 31 +/- 11 microEq/120 min) and 9-12 days (delta MAP = 11 +/- 3 mmHg, water ingestion = 3.3 +/- 0.9 ml/h and Na+ excretion = 37 +/- 11 microEq/120 min). Carbachol 0-9 microtubule-associated protein 6 Rattus norvegicus 67-74 2168484-9 1990 On the nACh-R, spermine, spermidine and agmatine inhibited [125I]alpha-bungarotoxin and also [3H]H12-HTX binding in presence of carbamylcholine. Carbachol 128-143 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 7-13 1706667-6 1990 SS-14 also attenuated secretory responses produced by carbachol, substance P (SP), VIP and alpha- and beta-calcitonin gene related peptide (alpha-, beta-CGRP). Carbachol 54-63 somatostatin Rattus norvegicus 0-5 2166590-0 1990 Activation of phospholipase C in rabbit brain membranes by carbachol in the presence of GTP gamma S; effects of biological detergents. Carbachol 59-68 LOC100009319 Oryctolagus cuniculus 14-29 2166590-4 1990 Optimal activation of phospholipase C by carbachol was seen at 10 to 100 nM free Ca2+. Carbachol 41-50 LOC100009319 Oryctolagus cuniculus 22-37 1980640-5 1990 Somatostatin inhibited carbachol- and cholecystokinin octapeptide-induced pepsinogen secretion from dispersed gastric mucosal cells in a dose-dependent manner. Carbachol 23-32 somatostatin Rattus norvegicus 0-12 2162335-2 1990 In all three tissue preparations, the cholinergic agonist carbamylcholine markedly inhibited the stimulation of cAMP biosynthesis by vasoactive intestinal peptide VIP--a potent activator of nonpigmented ciliary epithelial adenylate cyclase. Carbachol 58-73 VIP peptides Oryctolagus cuniculus 163-166 2162335-4 1990 The effects of carbamylcholine on VIP-induced cAMP synthesis were concentration dependent (EC50 = 23 nM), mimicked by selective muscarinic cholinergic agonists (oxotremorine, pilocarpine), and antagonized by atropine. Carbachol 15-30 VIP peptides Oryctolagus cuniculus 34-37 2162335-5 1990 Carbamylcholine- and clonidine-mediated inhibition of VIP-stimulated cAMP accumulation in ciliary processes were nonadditive, indicating that inhibitory muscarinic and alpha 2-adrenergic receptors coexist on VIP-responsive target cells. Carbachol 0-15 VIP peptides Oryctolagus cuniculus 54-57 2162335-5 1990 Carbamylcholine- and clonidine-mediated inhibition of VIP-stimulated cAMP accumulation in ciliary processes were nonadditive, indicating that inhibitory muscarinic and alpha 2-adrenergic receptors coexist on VIP-responsive target cells. Carbachol 0-15 VIP peptides Oryctolagus cuniculus 208-211 1976755-6 1990 Pretreatment of cells with a lower concentration of MTX (0.3 ng/ml) also attenuated carbachol- and glutamate-induced IP formation, in a time-dependent manner, with a decrease observed after 30 min prestimulation, but failed to affect NE-, histamine-, 5-HT-, endothelin-1, and sarafotoxin S6b-induced responses. Carbachol 84-93 endothelin 1 Rattus norvegicus 258-270 2117049-6 1990 Quinacrine, a nonselective phospholipase A2 inhibitor, reduced the carbachol stimulation of adenylate cyclase activity, but this inhibition appeared to be competitive with a Ki of 0.2 microM. Carbachol 67-76 phospholipase A2 group IB Rattus norvegicus 27-43 2146244-1 1990 The purpose of this study was to measure airway and hemodynamic effects of atrial natriuretic peptide (ANP) and its efficacy in counteracting the changes in lung mechanics that occur with aerosol histamine and carbachol. Carbachol 210-219 natriuretic peptides A Ovis aries 75-101 2146244-1 1990 The purpose of this study was to measure airway and hemodynamic effects of atrial natriuretic peptide (ANP) and its efficacy in counteracting the changes in lung mechanics that occur with aerosol histamine and carbachol. Carbachol 210-219 natriuretic peptides A Ovis aries 103-106 2146244-5 1990 ANP was given as bolus injections of 1, 5, and 10 micrograms/kg 3 min after either the ED65Cdyn or ED200RL doses of histamine and carbachol, respectively, and the airway response was monitored for 10 min. Carbachol 130-139 natriuretic peptides A Ovis aries 0-3 2146244-6 1990 ANP significantly reversed the rise in RL after carbachol administration (n = 10). Carbachol 48-57 natriuretic peptides A Ovis aries 0-3 2165399-5 1990 Incubation with carbachol for 15 min caused a small increase of membrane-associated PK-C activity (15% increase, P less than 0.05) as compared with the potency of phorbol esters (PMA) (3-4-fold increase, P less than 0.01). Carbachol 16-25 proline rich transmembrane protein 2 Homo sapiens 84-88 2359403-2 1990 Upon incubation of the cells with the full agonist carbachol, a 4.5- to 5-fold increase in CaM in the cytosol was observed, from 126 ng of CaM to 629 ng of CaM. Carbachol 51-60 calmodulin 1 Homo sapiens 91-94 2238765-8 1990 Prior exposure of the pancrease to carbachol enhanced the glucose / GLP-1 (7-36)amide induced insulin release, but left the arginine stimulated secretion unaltered. Carbachol 35-44 glucagon Rattus norvegicus 68-73 2359403-2 1990 Upon incubation of the cells with the full agonist carbachol, a 4.5- to 5-fold increase in CaM in the cytosol was observed, from 126 ng of CaM to 629 ng of CaM. Carbachol 51-60 calmodulin 1 Homo sapiens 139-142 2359403-2 1990 Upon incubation of the cells with the full agonist carbachol, a 4.5- to 5-fold increase in CaM in the cytosol was observed, from 126 ng of CaM to 629 ng of CaM. Carbachol 51-60 calmodulin 1 Homo sapiens 139-142 2359403-7 1990 The maximal changes in CaM concentration in both membranes and cytosol occurred with 10 microM carbachol. Carbachol 95-104 calmodulin 1 Homo sapiens 23-26 2399112-2 1990 High K+ and carbachol induced a sustained increase in [Ca2+]cyt and muscle tension. Carbachol 12-21 carbonic anhydrase 2 Canis lupus familiaris 55-58 2399112-4 1990 Cumulative addition of carbachol induced greater contraction than high K+ at a given [Ca2+]cyt 12-Deoxyphorbol 13-isobutyrate (DPB) (50 nmol/l) induced a small sustained contraction with little effect on [Ca2+]cyt. Carbachol 23-32 carbonic anhydrase 2 Canis lupus familiaris 86-89 2399112-4 1990 Cumulative addition of carbachol induced greater contraction than high K+ at a given [Ca2+]cyt 12-Deoxyphorbol 13-isobutyrate (DPB) (50 nmol/l) induced a small sustained contraction with little effect on [Ca2+]cyt. Carbachol 23-32 carbonic anhydrase 2 Canis lupus familiaris 205-208 2399112-8 1990 Addition of 50 nmol/l or 1 mumol/l DPB in the presence of carbachol inhibited both [Ca2+]cyt and muscle tension. Carbachol 58-67 carbonic anhydrase 2 Canis lupus familiaris 84-87 2399112-10 1990 Verapamil inhibited the contraction and [Ca2+]cyt stimulated by high K+ and carbachol. Carbachol 76-85 carbonic anhydrase 2 Canis lupus familiaris 41-44 2399112-11 1990 Isoprenaline and forskolin inhibited the high-K(+)-induced contraction without changing [Ca2+]cyt, whereas these inhibitors inhibited carbachol-induced contraction with a relatively small decrease in [Ca2+]cyt. Carbachol 134-143 carbonic anhydrase 2 Canis lupus familiaris 201-204 2399112-12 1990 These results suggest that (a) sustained contractions induced by high K+ and carbachol are due to the sustained increase in [Ca2+]cyt, (b) carbachol increases the sensitivity of contractile elements to Ca2+, and (c) isoprenaline and forskolin inhibit the contraction by the decrease in [Ca2+]cyt and also by the decrease in the sensitivity of contractile elements to Ca2+. Carbachol 77-86 carbonic anhydrase 2 Canis lupus familiaris 125-128 2399112-12 1990 These results suggest that (a) sustained contractions induced by high K+ and carbachol are due to the sustained increase in [Ca2+]cyt, (b) carbachol increases the sensitivity of contractile elements to Ca2+, and (c) isoprenaline and forskolin inhibit the contraction by the decrease in [Ca2+]cyt and also by the decrease in the sensitivity of contractile elements to Ca2+. Carbachol 139-148 carbonic anhydrase 2 Canis lupus familiaris 125-128 2399112-12 1990 These results suggest that (a) sustained contractions induced by high K+ and carbachol are due to the sustained increase in [Ca2+]cyt, (b) carbachol increases the sensitivity of contractile elements to Ca2+, and (c) isoprenaline and forskolin inhibit the contraction by the decrease in [Ca2+]cyt and also by the decrease in the sensitivity of contractile elements to Ca2+. Carbachol 139-148 carbonic anhydrase 2 Canis lupus familiaris 202-205 2399112-12 1990 These results suggest that (a) sustained contractions induced by high K+ and carbachol are due to the sustained increase in [Ca2+]cyt, (b) carbachol increases the sensitivity of contractile elements to Ca2+, and (c) isoprenaline and forskolin inhibit the contraction by the decrease in [Ca2+]cyt and also by the decrease in the sensitivity of contractile elements to Ca2+. Carbachol 139-148 carbonic anhydrase 2 Canis lupus familiaris 202-205 2399112-12 1990 These results suggest that (a) sustained contractions induced by high K+ and carbachol are due to the sustained increase in [Ca2+]cyt, (b) carbachol increases the sensitivity of contractile elements to Ca2+, and (c) isoprenaline and forskolin inhibit the contraction by the decrease in [Ca2+]cyt and also by the decrease in the sensitivity of contractile elements to Ca2+. Carbachol 139-148 carbonic anhydrase 2 Canis lupus familiaris 202-205 2359403-9 1990 Thus, the partial agonists were less efficacious than carbachol in eliciting changes in CaM localization. Carbachol 54-63 calmodulin 1 Homo sapiens 88-91 2162060-7 1990 The carbachol inhibitory effect occurred under conditions that prevented activation of phospholipase A2, excluding a role of the arachidonic acid metabolism in this process. Carbachol 4-13 phospholipase A2 group IB Rattus norvegicus 87-103 2167230-5 1990 In contrast, neuraminidase or virus decreased the affinity of carbachol for both sites in the heart. Carbachol 62-71 neuraminidase 1 Homo sapiens 13-26 2167230-6 1990 The neuraminidase inhibitor completely blocked virus-induced changes in carbachol affinity in both tissues. Carbachol 72-81 neuraminidase 1 Homo sapiens 4-17 2194793-4 1990 The transformed line secretes an apparently fully active enzyme and responds to carbachol stimulation with a rapid release of renin activity. Carbachol 80-89 renin Rattus norvegicus 126-131 1693390-5 1990 Carbachol-induced dopamine release was inhibited by substance P and by neuropeptide Y. Carbachol 0-9 tachykinin precursor 1 Bos taurus 52-63 2324742-7 1990 Under similar conditions, 0.5 mM carbachol stimulated the production of DG to the same extent as 200 ng/ml NGF and 50 ng/ml bFGF. Carbachol 33-42 nerve growth factor Rattus norvegicus 107-110 2324742-7 1990 Under similar conditions, 0.5 mM carbachol stimulated the production of DG to the same extent as 200 ng/ml NGF and 50 ng/ml bFGF. Carbachol 33-42 fibroblast growth factor 2 Rattus norvegicus 124-128 2324742-8 1990 Because carbachol is much more effective in stimulating the production of inositol phosphates, the results suggest that both NGF and bFGF stimulate the production of DG primarily from phospholipids other than the phosphoinositides. Carbachol 8-17 nerve growth factor Rattus norvegicus 125-128 1696375-2 1990 GAL-induced contractions (GAL EC50 = 9 nmol/l) were maximally 25% of those elicited by 10 mumol/l carbamylcholine and remained unaffected by atropine, tetrodotoxin, or tachyphylaxis to substance P. The presynaptic Ca2+ channel blocker, omega-conotoxin (0.1 mumol/l), inhibited GAL-induced contractions by 66%. Carbachol 98-113 galanin and GMAP prepropeptide Homo sapiens 0-3 2159581-5 1990 When nicotinic acetylcholine receptor was eluted with either carbachol or nicotine from the affinity column, these major bands were found on SDS-PAGE gels. Carbachol 61-70 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 5-37 2162944-7 1990 Incubation with carbachol, ATP or phorbol 12-myristate, 13-acetate desensitized the subsequent [Ca2+]i response to neurotensin. Carbachol 16-25 neurotensin Homo sapiens 115-126 1692606-3 1990 Neuraminidase decreased the affinity of the M2-selective agonist carbamylcholine in competitive binding experiments performed with [3H]QNB and [3H]NMS. Carbachol 65-80 neuraminidase 1 Homo sapiens 0-13 2110452-7 1990 Whereas VIP is reported to stimulate chloride secretion more strongly than carbachol, it was less effective than carbachol in stimulating mucin secretion. Carbachol 113-122 LOC100508689 Homo sapiens 138-143 1692187-2 1990 In this fraction, basal gastrin release (mean +/- SE) was 31.1 +/- 1.3 pg.10(6) cells-1.60 min-1 and was stimulated by 10(-8) M neuromedin C (222.3 +/- 18.1% of basal), 10(-4) M carbachol (227.5 +/- 25.9%), 10(-6) M 12-O-tetradecanoylphorbol-13-acetate (TPA) (196.3 +/- 14.7%), and 10(-3) M dibutyryl adenosine 3",5"-cyclic monophosphate (DBcAMP) (193.9 +/- 6.8%), respectively. Carbachol 178-187 gastrin Rattus norvegicus 24-31 1693390-5 1990 Carbachol-induced dopamine release was inhibited by substance P and by neuropeptide Y. Carbachol 0-9 neuropeptide Y Bos taurus 71-85 2352839-1 1990 The effects of K+ depolarization and of the muscarinic agonist carbachol on [Ca2+]i and force were investigated in smooth muscle sheets of the longitudinal layer of the ileum loaded with Fura-2. Carbachol 63-72 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 77-80 2352839-6 1990 The stimulation with 0.1 mM carbachol resulted in a transient increase of [Ca2+]i and force followed by a continuous decline of these parameters despite the presence of the drug. Carbachol 28-37 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 75-78 2352839-10 1990 These results suggest that (a) both agonists increase force and [Ca2+]i during stimulation; (b) during depolarization with K+, desensitization to CA2+ occurs resulting in a clockwise hysteresis loop; (c) during carbachol stimulation, a counterclockwise hysteresis is observed. Carbachol 211-220 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 146-149 2155219-9 1990 The calmodulin inhibitors trifluoperazine and W7 (10(-4) M) prevented the stimulation of the exchanger induced by carbachol or epinephrine, but W7 could not block the stimulation produced by A23187 arguing against the involvement of calmodulin in this effect. Carbachol 114-123 calmodulin 1 Rattus norvegicus 4-14 2307120-8 1990 Vascular bombesin (10(-7) M) and carbachol (10(-5) M) provoked a biphasic release of NT, consisting of a transient rise (approximately 600% above basal) followed by a less pronounced but sustained response. Carbachol 33-42 neurotensin Rattus norvegicus 85-87 2156987-2 1990 The pD2 value of BK on the longitudinal muscle was 6.58 +/- 0.06 M. The maximal response of the longitudinal muscle to BK was 44% compared to carbachol. Carbachol 142-151 kininogen 1 Homo sapiens 17-19 1968059-4 1990 Moreover, somatostatin significantly inhibited gene transcription that had been stimulated by dibutyryl-cAMP and carbachol. Carbachol 113-122 somatostatin Canis lupus familiaris 10-22 1689544-3 1990 Stimulation with the cholinergic agonist carbachol (1 microM) resulted in a rapid increase in [Ca2+]i to 308 +/- 26 nM, which was sustained at a nearly constant elevated level (328 +/- 31 nM) throughout agonist application. Carbachol 41-50 carbonic anhydrase 2 Homo sapiens 95-98 2157926-5 1990 Thus, the main features of AD are a cAarP response to Met-enk and an abolition of a GarP response to carbachol. Carbachol 101-110 leucine rich repeat containing 32 Homo sapiens 84-88 2156444-2 1990 EGF effects on histamine- and carbachol-stimulated [14C]aminopyrine (AP) uptake and intrinsic factor (IF) secretion were evaluated in isolated rabbit parietal cells. Carbachol 30-39 pro-epidermal growth factor Oryctolagus cuniculus 0-3 2156444-6 1990 EGF inhibited carbachol-stimulated [14C]AP uptake and IF secretion, but did not alter the carbachol-stimulated Ca2+ transient. Carbachol 14-23 pro-epidermal growth factor Oryctolagus cuniculus 0-3 2156444-7 1990 These results indicate that EGF inhibits histamine-stimulated secretion through the inhibitory Gi guanosine 5"-triphosphate-binding protein and carbachol-stimulated secretion through a mechanism independent of the activation of an increase in intracellular Ca2+. Carbachol 144-153 pro-epidermal growth factor Oryctolagus cuniculus 28-31 2106265-3 1990 When membrane potential was clamped to 0 mV, carbachol induced an outwardly directed K+ current in 31 of 37 cells, with a peak value of 618 +/- 51 (SE) pA. Carbachol 45-54 extra spindle pole bodies like 1, separase Homo sapiens 148-154 2156059-4 1990 Prolonged incubation of SK-N-SH cells with the partial agonist bethanechol also resulted in a desensitization of stimulated PPI turnover but at a slower rate than that observed for carbachol and with a loss of fewer mAChR sites. Carbachol 181-190 hedgehog acyltransferase Homo sapiens 24-28 2304648-2 1990 Intracellular recordings from CA1 pyramidal neurones revealed that CCh (1-3 microM) inhibited excitatory postsynaptic responses evoked by stimulation of the Schaffer collateral/commissural pathway while, at the same time, direct excitability was enhanced. Carbachol 67-70 carbonic anhydrase 1 Rattus norvegicus 30-33 2304648-3 1990 Extracellularly, CCh produced a concentration-dependent reduction of the amplitude of the field excitatory postsynaptic potential (field EPSP) recorded in the CA1 apical dendritic region. Carbachol 17-20 carbonic anhydrase 1 Rattus norvegicus 159-162 2304648-5 1990 These results confirm earlier reports of a presynaptic inhibitory action of CCh in the hippocampal CA1 region and provide strong evidence that this effect is mediated by muscarinic receptors of the M1 subtype. Carbachol 76-79 carbonic anhydrase 1 Rattus norvegicus 99-102 2302186-2 1990 Carbachol stimulation leads to a rapid increase in intracellular Ca2+ and Ins(1,4,5)P3 mass, both reaching a peak at around 10 s and then declining to a new maintained phase significantly above basal. Carbachol 0-9 carbonic anhydrase 2 Homo sapiens 65-68 2302186-3 1990 Dose-response analysis of peak and plateau phases of intracellular Ca2+ shows different agonist potencies for both phases, carbachol being more potent for the plateau phase. Carbachol 123-132 carbonic anhydrase 2 Homo sapiens 67-70 2153579-3 1990 The inhibitory effect of mastoparan on carbachol-induced [3H]inositol phosphate accumulation was resistant to pertussis toxin (IAP) treatment in intact cells. Carbachol 39-48 alkaline phosphatase, intestinal Homo sapiens 127-130 1689117-1 1990 First incubating guinea pig pancreatic acini with carbachol reduced the subsequent stimulation of amylase release caused by carbachol, cholecystokinin octapeptide (CCK-8), and bombesin but not that caused by vasoactive intestinal peptide, substance P, 8-bromoadenosine 3",5"-cyclic monophosphate, A23187, or 12-O-tetradecanoylphorbol-13-acetate. Carbachol 50-59 cholecystokinin Cavia porcellus 164-167 1689117-2 1990 Carbachol also reduced the subsequent binding of N-[3H]methylscopolamine, 125I-CCK-8, and 125I-[Tyr4]bombesin. Carbachol 0-9 cholecystokinin Cavia porcellus 79-82 1689117-5 1990 Carbachol occupation of low-affinity cholinergic receptors appears to produce the reduction in CCK-8- and bombesin-stimulated amylase release and in binding of 125I-CCK-8 and 125I-[Tyr4]bombesin. Carbachol 0-9 cholecystokinin Cavia porcellus 95-98 1689117-5 1990 Carbachol occupation of low-affinity cholinergic receptors appears to produce the reduction in CCK-8- and bombesin-stimulated amylase release and in binding of 125I-CCK-8 and 125I-[Tyr4]bombesin. Carbachol 0-9 cholecystokinin Cavia porcellus 165-168 1689117-8 1990 First incubating acini with carbachol caused a 60% decrease in the number of high-affinity CCK receptors with no change in the number of low-affinity receptors or the affinities of either class of receptors for CCK-8. Carbachol 28-37 cholecystokinin Cavia porcellus 91-94 1689117-10 1990 12-O-tetradecanoyl phorbol-13-acetate reproduced the action of carbachol on binding of N-[3H]methylscopolamine and 125I-CCK-8 but not on binding of 125I-[Tyr4]bombesin, suggesting that carbachol activation of protein kinase C may in some way mediate the effect of carbachol on receptors for carbachol and those for CCK but not that on receptors for bombesin. Carbachol 63-72 cholecystokinin Cavia porcellus 120-123 1689117-10 1990 12-O-tetradecanoyl phorbol-13-acetate reproduced the action of carbachol on binding of N-[3H]methylscopolamine and 125I-CCK-8 but not on binding of 125I-[Tyr4]bombesin, suggesting that carbachol activation of protein kinase C may in some way mediate the effect of carbachol on receptors for carbachol and those for CCK but not that on receptors for bombesin. Carbachol 63-72 cholecystokinin Cavia porcellus 315-318 2269025-18 1990 LHRH can have a modulatory role upon the action of 5-HT, DA and Carb, and estrogen pretreatment specifically affects that of DA in the SCN. Carbachol 64-68 gonadotropin releasing hormone 1 Rattus norvegicus 0-4 2400633-7 1990 In conclusion, phospholipase C activation, intracellular Ca2+ release and aminopyrine accumulation were sequentially observed following carbachol stimulation of the isolated gastric parietal cell and extracellular calcium contributed to sustain this acid secretory response. Carbachol 136-145 LOC100009319 Oryctolagus cuniculus 15-30 1702070-1 1990 Chornic exogenous administration of cholecystokinin octapeptide (CCK8) to rats led to a reduced sensitivity of pancreatic acinar cells to both CCK8 and carbachol stimulation without changes in affinity or number of CCK or muscarinic receptors. Carbachol 152-161 cholecystokinin Rattus norvegicus 65-68 2175722-4 1990 Carbachol occupation of low affinity cholinergic receptors appears to produce a reduction in binding of 125I-CCK-8 and 125I-[Tyr4]bombesin. Carbachol 0-9 gastrin releasing peptide Homo sapiens 130-138 2175722-8 1990 Acini possess a single class of bombesin receptors and first incubating acini with carbachol caused a 40% decrease in the number of bombesin receptors with no change in their affinity for bombesin. Carbachol 83-92 gastrin releasing peptide Homo sapiens 32-40 2175722-8 1990 Acini possess a single class of bombesin receptors and first incubating acini with carbachol caused a 40% decrease in the number of bombesin receptors with no change in their affinity for bombesin. Carbachol 83-92 gastrin releasing peptide Homo sapiens 132-140 2175722-8 1990 Acini possess a single class of bombesin receptors and first incubating acini with carbachol caused a 40% decrease in the number of bombesin receptors with no change in their affinity for bombesin. Carbachol 83-92 gastrin releasing peptide Homo sapiens 132-140 2282996-1 1990 This study was designed to investigate the relationship between estrogen and progesterone receptor levels and in vitro contractile response of gallbladder muscle strips to stimulation by carbachol and cholecystokinin-octapeptide (CCK-OP). Carbachol 187-196 progesterone receptor Homo sapiens 77-98 2282996-5 1990 Positive correlations were found between the progesterone receptor level and the carbachol concentration that produced half the maximal response (ED50), and between the estrogen receptor level and the ED50 of CCK-OP. Carbachol 81-90 progesterone receptor Homo sapiens 45-66 1688390-1 1990 Pretreatment of rat pancreatic acini with phorbol 12-myristate, 13-acetate (PMA), a protein kinase C (PK-C) activator, caused the desensitization of carbamylcholine (CBC)-induced amylase release in a concentration- and time-dependent fashion. Carbachol 149-164 protein kinase C, gamma Rattus norvegicus 84-100 2153134-2 1990 In testing the possibility that VGCC may be functionally coupled to mAChR in SCC cell lines, we found that depolarization-dependent Ca2+ influx was inhibited by carbachol (IC50 = 0.78 microM) and oxotremorine (IC50 = 0.69 microM). Carbachol 161-170 serpin family B member 3 Homo sapiens 77-80 2153134-4 1990 Exposure of SCC to carbachol induced the hydrolysis of phosphoinositides and increased the cytosolic free Ca2+ concentration ([Ca2+]i). Carbachol 19-28 serpin family B member 3 Homo sapiens 12-15 33765249-4 2022 We found GlyT2 is down-regulated by carbachol in a calcium-dependent manner. Carbachol 36-45 solute carrier family 6 member 5 Homo sapiens 9-14 2293258-9 1990 On the other hand, intracerebral injections of physostigmine (100 micrograms/microliter), an acetylcholinesterase inhibitor which elevates the level of endogenous acetylcholine, induced the fully developed emotional-aversive response comparable with natural behaviour and with responses induced by carbachol (10 micrograms/microliter). Carbachol 298-307 acetylcholinesterase Felis catus 93-113 33808507-3 2021 The current study showed that the release of a luciferase from a differentiated human neuroblastoma-based reporter cell line (SIMA-hPOMC1-26-GLuc cells) can be stimulated by a carbachol-mediated activation of the Gq-coupled muscarinic-acetylcholine receptor and by the Ca2+-channel forming spider toxin alpha-latrotoxin. Carbachol 176-185 glucosylceramidase beta 3 (gene/pseudogene) Homo sapiens 141-145 2130511-18 1990 Activation of phospholipase D (PLD) was demonstrated by the finding that phosphatidic acid increased in response to PMA or carbachol prior to the increase in PA. Carbachol 123-132 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 14-29 2130511-18 1990 Activation of phospholipase D (PLD) was demonstrated by the finding that phosphatidic acid increased in response to PMA or carbachol prior to the increase in PA. Carbachol 123-132 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 31-34 33765249-6 2022 GlyT2 down-regulation by carbachol was increased by thapsigargin and reduced by internal store depletion, although calcium release from endoplasmic reticulum or mitochondria had a minor role on GlyT2 inhibition. Carbachol 25-34 solute carrier family 6 member 5 Homo sapiens 0-5 12384253-7 2002 In another set of experiments, the NK(1) receptor antagonist L-703-606 (5 microg) was microinjected near the A7 cell group following LH stimulation with carbachol. Carbachol 153-162 tachykinin receptor 1 Rattus norvegicus 35-49 23744739-6 2013 Carbachol and cAMP redistributed CFTR to the apical membrane. Carbachol 0-9 CF transmembrane conductance regulator Homo sapiens 33-37 34878647-7 2022 Carbachol and a muscarinic M1 receptor (M1R) agonist facilitated KCNQ2 phosphorylation at T217 in NAc/striatum slices in a PKC-dependent manner. Carbachol 0-9 potassium voltage-gated channel, subfamily Q, member 2 Mus musculus 65-70 7925360-6 1994 Short-term (30 min) incubation with carbachol (1 mM) induced a simultaneous transfer of a proportion of both Gq alpha and G11 alpha and Hm1 receptors from plasma membranes to distinct light vesicular membranes. Carbachol 36-45 cholinergic receptor muscarinic 1 Homo sapiens 136-139 34418586-8 2022 Carbachol and PGE2 mediated mucin stimulation was examined by MUC2 IF/confocal microscopy and TEM. Carbachol 0-9 mucin 2, oligomeric mucus/gel-forming Homo sapiens 62-66 34370080-10 2021 Application of the cholinomimetic drug carbachol or blocking M2R with methoctramine significantly promoted mucus secretion by goblet cells and increased MUC2 content in duodenal mucosa-incubated solutions from 6-OHDA and vagotomy rats. Carbachol 39-48 mucin 2, oligomeric mucus/gel-forming Rattus norvegicus 153-157 34415562-10 2022 RESULTS: We found that carbachol increased the expression of NLRP3 inflammasome (NLRP3, ASC, cleaved caspase-1, IL-1beta, and IL-18). Carbachol 23-32 NLR family pyrin domain containing 3 Homo sapiens 61-66 34415562-10 2022 RESULTS: We found that carbachol increased the expression of NLRP3 inflammasome (NLRP3, ASC, cleaved caspase-1, IL-1beta, and IL-18). Carbachol 23-32 NLR family pyrin domain containing 3 Homo sapiens 81-86 34415562-10 2022 RESULTS: We found that carbachol increased the expression of NLRP3 inflammasome (NLRP3, ASC, cleaved caspase-1, IL-1beta, and IL-18). Carbachol 23-32 PYD and CARD domain containing Homo sapiens 88-91 34415562-10 2022 RESULTS: We found that carbachol increased the expression of NLRP3 inflammasome (NLRP3, ASC, cleaved caspase-1, IL-1beta, and IL-18). Carbachol 23-32 caspase 1 Homo sapiens 101-110 34415562-10 2022 RESULTS: We found that carbachol increased the expression of NLRP3 inflammasome (NLRP3, ASC, cleaved caspase-1, IL-1beta, and IL-18). Carbachol 23-32 interleukin 1 alpha Homo sapiens 112-120 34415562-10 2022 RESULTS: We found that carbachol increased the expression of NLRP3 inflammasome (NLRP3, ASC, cleaved caspase-1, IL-1beta, and IL-18). Carbachol 23-32 interleukin 18 Homo sapiens 126-131 34415562-13 2022 The addition of RA also significantly reduced the effect of carbachol on NLRP3 inflammasomes. Carbachol 60-69 NLR family pyrin domain containing 3 Homo sapiens 73-78 34415562-14 2022 CONCLUSIONS: Our current study suggests that carbachol induces NLRP3 inflammasome activation through mAChR and NF-kB, and that RA abolishes the inflammatory response. Carbachol 45-54 NLR family pyrin domain containing 3 Homo sapiens 63-68 34603302-11 2021 Similarly, disruption of IP3R/Ca2+ in MLE12 and RAW264.7 cells using carbachol lead to inflammatory responses, and stimulated ER stress and mitochondrial dysfunction. Carbachol 69-78 inositol 1,4,5-trisphosphate receptor 1 Mus musculus 25-29 34561235-3 2021 We show that cholinergic receptor activation with the non-selective cholinergic agonist, carbachol (CCh), triggers a protein synthesis-dependent and NMDAR-independent long-term synaptic depression (CCh-LTD) at entorhinal cortical (EC)-CA2 and Schaffer collateral (SC)-CA2 synapses in the hippocampus of adult male Wistar rats. Carbachol 89-98 carbonic anhydrase 2 Rattus norvegicus 235-238 34561235-3 2021 We show that cholinergic receptor activation with the non-selective cholinergic agonist, carbachol (CCh), triggers a protein synthesis-dependent and NMDAR-independent long-term synaptic depression (CCh-LTD) at entorhinal cortical (EC)-CA2 and Schaffer collateral (SC)-CA2 synapses in the hippocampus of adult male Wistar rats. Carbachol 89-98 carbonic anhydrase 2 Rattus norvegicus 268-271 34561235-3 2021 We show that cholinergic receptor activation with the non-selective cholinergic agonist, carbachol (CCh), triggers a protein synthesis-dependent and NMDAR-independent long-term synaptic depression (CCh-LTD) at entorhinal cortical (EC)-CA2 and Schaffer collateral (SC)-CA2 synapses in the hippocampus of adult male Wistar rats. Carbachol 100-103 carbonic anhydrase 2 Rattus norvegicus 235-238 34561235-3 2021 We show that cholinergic receptor activation with the non-selective cholinergic agonist, carbachol (CCh), triggers a protein synthesis-dependent and NMDAR-independent long-term synaptic depression (CCh-LTD) at entorhinal cortical (EC)-CA2 and Schaffer collateral (SC)-CA2 synapses in the hippocampus of adult male Wistar rats. Carbachol 100-103 carbonic anhydrase 2 Rattus norvegicus 268-271 34561235-4 2021 The activation of muscarinic acetylcholine receptors (mAChRs) is critical for the induction of CCh-LTD with the results suggesting an involvement of M3 and M1 mAChRs in the early facilitation of CCh-LTD, while nicotinic acetylcholine receptor activation plays a role in the late maintenance of CCh-LTD at CA2 synapses. Carbachol 95-98 carbonic anhydrase 2 Rattus norvegicus 305-308 34561235-4 2021 The activation of muscarinic acetylcholine receptors (mAChRs) is critical for the induction of CCh-LTD with the results suggesting an involvement of M3 and M1 mAChRs in the early facilitation of CCh-LTD, while nicotinic acetylcholine receptor activation plays a role in the late maintenance of CCh-LTD at CA2 synapses. Carbachol 294-297 carbonic anhydrase 2 Rattus norvegicus 305-308 34561235-5 2021 Remarkably, we find that CCh priming lowers the threshold for the subsequent induction of persistent long-term potentiation (LTP) of synaptic transmission at EC-CA2 and the plasticity-resistant SC-CA2 pathways. Carbachol 25-28 carbonic anhydrase 2 Rattus norvegicus 161-164 34561235-5 2021 Remarkably, we find that CCh priming lowers the threshold for the subsequent induction of persistent long-term potentiation (LTP) of synaptic transmission at EC-CA2 and the plasticity-resistant SC-CA2 pathways. Carbachol 25-28 carbonic anhydrase 2 Rattus norvegicus 197-200 34561235-8 2021 Moreover, the reinforcement of LTP at EC inputs to CA2 following the priming stimulus co-exists with concurrent sustained CCh-LTD at the SC-CA2 pathway and is dynamically scaled by modulation of SC-CA2 synaptic transmission.Significance Statement:The release of the neuromodulator acetylcholine is critically involved in processes of hippocampus-dependent memory formation. Carbachol 122-125 carbonic anhydrase 2 Rattus norvegicus 140-143 34671658-6 2021 At 48 and 60 h, 10-5 M carbachol was used to stimulate gastrin secretion. Carbachol 23-32 gastrin Equus caballus 55-62 34671658-9 2021 Carbachol resulted in a significant increase in gastrin concentration in CO and DF treatments, but not in BL. Carbachol 0-9 gastrin Equus caballus 48-55 34487726-7 2021 CCh stimulation significantly elevates intracellular (Ca2+), an effect associated with increases in the size of Rab3D-enriched vesicles consistent with compound fusion, and subsequently decreases in their intensity of labeling consistent with their exocytosis. Carbachol 0-3 RAB3D, member RAS oncogene family Mus musculus 112-117 34487726-10 2021 Significant increases in the colocalization of endolysosomal vesicle markers (Lamp1, Lamp2, Rab7) with the subapical actin suggestive of fusion of endolysosomal vesicles at the apical membrane occur both with CCh and PE stimulation, but PE demonstrates increased colocalization. Carbachol 209-212 lysosomal-associated membrane protein 1 Mus musculus 78-83 34487726-10 2021 Significant increases in the colocalization of endolysosomal vesicle markers (Lamp1, Lamp2, Rab7) with the subapical actin suggestive of fusion of endolysosomal vesicles at the apical membrane occur both with CCh and PE stimulation, but PE demonstrates increased colocalization. Carbachol 209-212 lysosomal-associated membrane protein 2 Mus musculus 85-90 34487726-10 2021 Significant increases in the colocalization of endolysosomal vesicle markers (Lamp1, Lamp2, Rab7) with the subapical actin suggestive of fusion of endolysosomal vesicles at the apical membrane occur both with CCh and PE stimulation, but PE demonstrates increased colocalization. Carbachol 209-212 RAB7, member RAS oncogene family Mus musculus 92-96 34576086-7 2021 Accordingly, CRP4 KO mice presented with hypotonia at baseline, as well as a greater drop in systolic BP in response to the acute administration of cinaciguat, sodium nitroprusside, and carbachol. Carbachol 186-195 cysteine rich protein 2 Mus musculus 13-17 34539433-7 2021 Accordingly, MRTF-B conferred responsiveness to the muscarinic agonist carbachol in Ca2+ imaging experiments. Carbachol 71-80 myocardin related transcription factor B Homo sapiens 13-19 34311523-9 2021 The major findings of the study reveal that- compared to the nondiabetic controls - the diabetic animals CC showed: (1) augmented contraction and attenuated relaxation in response to phenylephrine and carbachol, respectively, (2) marked fibromuscular degeneration with a significantly lower smooth muscle/collagen ratio and upregulation of TGF-beta-1/Smad/CTGF fibrosis signaling pathway, (3) reduced H2S plasma and urinary levels and cavernosal tissue generation. Carbachol 201-210 transforming growth factor, beta 1 Rattus norvegicus 340-350 34512394-11 2021 CCh inhibited CaMKII activity, whereas CaMKII inhibition with KN93 mimicked the effect of CCh on Ca2+ transients in formamide-treated myocytes. Carbachol 0-3 calcium/calmodulin-dependent protein kinase II, delta Mus musculus 14-20 34177470-7 2021 In addition to theta power and frequency, intraseptal NBQX also attenuated suppression of CA1 population spike (PS) induced by intraseptal carbachol, thus suggesting that septal glutamate neurotransmission is involved in the spectrum of MS-mediated network responses. Carbachol 139-148 carbonic anhydrase 1 Rattus norvegicus 90-93 34588752-6 2021 METHODS: In freshly dispersed smooth muscle cells (SMC), we measured the adiponectin effect on the carbachol-induced length changes. Carbachol 99-108 adiponectin, C1Q and collagen domain containing Rattus norvegicus 73-84 34588752-7 2021 RESULTS: Adiponectin significantly reduced the carbachol-stimulated SMC shortening independently of age. Carbachol 47-56 adiponectin, C1Q and collagen domain containing Rattus norvegicus 9-20 34180063-7 2021 Introduction of a non-phosphorylatable S675A mutant of beta-catenin into myoblasts impaired carbachol, a Rac1 and RhoA activator, stimulated GLUT4 translocation and actin remodelling in myoblasts, and exercise-induced increases in cross-sectional phalloidin staining (F-actin marker) of gastrocnemius muscle was impaired in muscle from BCAT-mKO. Carbachol 92-101 catenin (cadherin associated protein), beta 1 Mus musculus 55-67 34180063-7 2021 Introduction of a non-phosphorylatable S675A mutant of beta-catenin into myoblasts impaired carbachol, a Rac1 and RhoA activator, stimulated GLUT4 translocation and actin remodelling in myoblasts, and exercise-induced increases in cross-sectional phalloidin staining (F-actin marker) of gastrocnemius muscle was impaired in muscle from BCAT-mKO. Carbachol 92-101 Rac family small GTPase 1 Mus musculus 105-109 34180063-7 2021 Introduction of a non-phosphorylatable S675A mutant of beta-catenin into myoblasts impaired carbachol, a Rac1 and RhoA activator, stimulated GLUT4 translocation and actin remodelling in myoblasts, and exercise-induced increases in cross-sectional phalloidin staining (F-actin marker) of gastrocnemius muscle was impaired in muscle from BCAT-mKO. Carbachol 92-101 ras homolog family member A Mus musculus 114-118 34180063-7 2021 Introduction of a non-phosphorylatable S675A mutant of beta-catenin into myoblasts impaired carbachol, a Rac1 and RhoA activator, stimulated GLUT4 translocation and actin remodelling in myoblasts, and exercise-induced increases in cross-sectional phalloidin staining (F-actin marker) of gastrocnemius muscle was impaired in muscle from BCAT-mKO. Carbachol 92-101 solute carrier family 2 (facilitated glucose transporter), member 4 Mus musculus 141-146 34385927-7 2021 Then, we investigated the role and mechanisms of MALAT1 in the proliferation, migration, phenotypic switch, and carbachol-induced intracellular Ca2+ changes in human gastric smooth muscle cells (HGSMCs) under high glucose (HG) conditions, using short hairpin RNA technology, RNA immunoprecipitation, and dual-luciferase reporter assays. Carbachol 112-121 metastasis associated lung adenocarcinoma transcript 1 Homo sapiens 49-55 34385927-10 2021 Additionally, we show that MALAT1 sponged miR-449a, regulating Delta-like ligand 1 (DLL1) expression in HGSMCs; any disturbance of the MALAT1/miR-449a/DLL1 pathway affects the proliferation, migration, phenotypic switch, and carbachol-induced Ca2+ transient signals in HGSMCs under HG conditions. Carbachol 225-234 metastasis associated lung adenocarcinoma transcript 1 Homo sapiens 27-33 34311523-9 2021 The major findings of the study reveal that- compared to the nondiabetic controls - the diabetic animals CC showed: (1) augmented contraction and attenuated relaxation in response to phenylephrine and carbachol, respectively, (2) marked fibromuscular degeneration with a significantly lower smooth muscle/collagen ratio and upregulation of TGF-beta-1/Smad/CTGF fibrosis signaling pathway, (3) reduced H2S plasma and urinary levels and cavernosal tissue generation. Carbachol 201-210 cellular communication network factor 2 Rattus norvegicus 356-360 35427599-6 2022 qPCR and whole-mount immunohistochemical analysis in ex vivo organ culture system revealed that SG epithelial cells near a parasympathetic nerve were found to be induced to differentiate into MECs via the cholinergic receptor muscarinic 1 by carbachol (CCh), an acetylcholine agonist. Carbachol 242-251 cholinergic receptor, muscarinic 1 Rattus norvegicus 205-238 35427599-6 2022 qPCR and whole-mount immunohistochemical analysis in ex vivo organ culture system revealed that SG epithelial cells near a parasympathetic nerve were found to be induced to differentiate into MECs via the cholinergic receptor muscarinic 1 by carbachol (CCh), an acetylcholine agonist. Carbachol 253-256 cholinergic receptor, muscarinic 1 Rattus norvegicus 205-238 35427599-7 2022 In addition, CCh stimulated ERK and Akt signaling for the induction of MEC differentiation in rat submandibular gland epithelial cells. Carbachol 13-16 Eph receptor B1 Rattus norvegicus 28-31 35427599-7 2022 In addition, CCh stimulated ERK and Akt signaling for the induction of MEC differentiation in rat submandibular gland epithelial cells. Carbachol 13-16 AKT serine/threonine kinase 1 Rattus norvegicus 36-39 35431993-4 2022 The absence of extracellular Ca2+ influx after immersion into nominally zero Ca2+ solution, or the addition of nifedipine, significantly inhibited the contractile responses (p < 0.05 for all) after stimulation with carbachol (1 microM), histamine (100 microM), 5-hydroxytryptamine (100 microM), neurokinin-A (300 nM), prostaglandin E2 (10 microM), and angiotensin II (100 nM). Carbachol 215-224 tachykinin precursor 1 Homo sapiens 295-307 35088452-9 2022 We found that exposure to propranolol either by combination with carbachol facilitates additive effects on inhibition of caspase 3 and 8 expression in chronic myeloid leukemia K562 cells. Carbachol 65-74 caspase 3 Homo sapiens 121-136 35088452-10 2022 But caspase 9 expression level was increased by propranolol alone or with propranolol and Carbachol combination. Carbachol 90-99 caspase 9 Homo sapiens 4-13 35431993-4 2022 The absence of extracellular Ca2+ influx after immersion into nominally zero Ca2+ solution, or the addition of nifedipine, significantly inhibited the contractile responses (p < 0.05 for all) after stimulation with carbachol (1 microM), histamine (100 microM), 5-hydroxytryptamine (100 microM), neurokinin-A (300 nM), prostaglandin E2 (10 microM), and angiotensin II (100 nM). Carbachol 215-224 angiotensinogen Homo sapiens 352-366 2531900-5 1989 Injection of carbachol into the AV3V produced the expected natriuresis, which was accompanied within 20 min by a dramatic rise in the plasma ANP concentration and a rise in ANP content in the medial basal hypothalamus, the neurohypophysis, and particularly the anterior hypophysis but without alterations in the content of ANP in the lungs or the right or left atrium. Carbachol 13-22 natriuretic peptide A Rattus norvegicus 141-144 35307777-7 2022 ALFA-tagged ArcNb also provided efficient immunoprecipitation of stimulus-induced Arc after carbachol-treatment of SH-SY5Y neuroblastoma cells and induction of long-term potentiation in the rat dentate gyrus in vivo. Carbachol 92-101 activity regulated cytoskeleton associated protein Homo sapiens 82-85 2557126-2 1989 Carbachol concentration-dependently induced the hydrolysis of inositol lipids and formation of [3H]IP3, [3H]IP2 and [3H]IP1 in these cells labeled with [3H]inositol. Carbachol 0-9 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 108-111 2690956-4 1989 pHi remained unaffected after raising the glucose concentration from 3 to 20 mM, it was lowered when depolarizing the beta-cells with tolbutamide and it increased in the presence of carbachol. Carbachol 182-191 glucose-6-phosphate isomerase 1 Mus musculus 0-3 2514601-0 1989 Carbachol-induced cytosolic free Ca2+ increases in T84 colonic cells seen by microfluorimetry. Carbachol 0-9 carbonic anhydrase 2 Homo sapiens 33-36 2514601-1 1989 Changes in cytosolic free Ca2+ ([Ca2+]i) in response to the secretagogue carbachol have been characterized in the human colon cancer cell line T84, a model Cl- secretory cell. Carbachol 73-82 carbonic anhydrase 2 Homo sapiens 26-29 2514601-1 1989 Changes in cytosolic free Ca2+ ([Ca2+]i) in response to the secretagogue carbachol have been characterized in the human colon cancer cell line T84, a model Cl- secretory cell. Carbachol 73-82 carbonic anhydrase 2 Homo sapiens 33-36 2514601-4 1989 The cholinergic agonist carbachol caused a concentration-dependent rise in [Ca2+]i with a Km of 4 microM and a peak increase in [Ca2+]i of approximately 50 nM. Carbachol 24-33 carbonic anhydrase 2 Homo sapiens 76-79 2514601-4 1989 The cholinergic agonist carbachol caused a concentration-dependent rise in [Ca2+]i with a Km of 4 microM and a peak increase in [Ca2+]i of approximately 50 nM. Carbachol 24-33 carbonic anhydrase 2 Homo sapiens 129-132 2514601-7 1989 In conclusion, the initial detectable increase in [Ca2+]i caused by carbachol is due to the release of Ca2+ from internal stores, whereas the contribution of extracellular Ca2+ occurs later and at least partially involves a nifedipine- and verapamil-sensitive process. Carbachol 68-77 carbonic anhydrase 2 Homo sapiens 51-54 2514601-7 1989 In conclusion, the initial detectable increase in [Ca2+]i caused by carbachol is due to the release of Ca2+ from internal stores, whereas the contribution of extracellular Ca2+ occurs later and at least partially involves a nifedipine- and verapamil-sensitive process. Carbachol 68-77 carbonic anhydrase 2 Homo sapiens 103-106 2514601-7 1989 In conclusion, the initial detectable increase in [Ca2+]i caused by carbachol is due to the release of Ca2+ from internal stores, whereas the contribution of extracellular Ca2+ occurs later and at least partially involves a nifedipine- and verapamil-sensitive process. Carbachol 68-77 carbonic anhydrase 2 Homo sapiens 103-106 2531900-5 1989 Injection of carbachol into the AV3V produced the expected natriuresis, which was accompanied within 20 min by a dramatic rise in the plasma ANP concentration and a rise in ANP content in the medial basal hypothalamus, the neurohypophysis, and particularly the anterior hypophysis but without alterations in the content of ANP in the lungs or the right or left atrium. Carbachol 13-22 natriuretic peptide A Rattus norvegicus 173-176 2531900-5 1989 Injection of carbachol into the AV3V produced the expected natriuresis, which was accompanied within 20 min by a dramatic rise in the plasma ANP concentration and a rise in ANP content in the medial basal hypothalamus, the neurohypophysis, and particularly the anterior hypophysis but without alterations in the content of ANP in the lungs or the right or left atrium. Carbachol 13-22 natriuretic peptide A Rattus norvegicus 173-176 2531900-12 1989 The dramatic increase in plasma ANP after carbachol stimulation of the AV3V that was accompanied by marked elevations in content of the peptide in basal hypothalamus and neuro- and adenohypophysis suggests that the natriuresis resulting from this stimulation is brought about at least in part by release of ANP from the brain. Carbachol 42-51 natriuretic peptide A Rattus norvegicus 32-35 2531900-12 1989 The dramatic increase in plasma ANP after carbachol stimulation of the AV3V that was accompanied by marked elevations in content of the peptide in basal hypothalamus and neuro- and adenohypophysis suggests that the natriuresis resulting from this stimulation is brought about at least in part by release of ANP from the brain. Carbachol 42-51 natriuretic peptide A Rattus norvegicus 307-310 2591535-1 1989 In intact smooth muscle strips from chicken gizzard, electrical stimulation and carbachol elicited brief, phasic contractions which were associated with a very rapid, transient phosphorylation of the 20 kDa myosin light chains. Carbachol 80-89 myosin, heavy chain 15 Gallus gallus 207-213 2556936-5 1989 The carbachol-induced changes in Isc appeared to be dependent on the increase in [Ca]i. Carbachol 4-13 carbonic anhydrase 1 Homo sapiens 82-86 2556936-13 1989 These experiments suggest that although the carbachol-induced increase in Isc is dependent on the increase in [Ca]i, the hormone may activate a second process that increases the sensitivity of the calcium-activated transport process to changes in [Ca]i. Carbachol 44-53 carbonic anhydrase 1 Homo sapiens 111-115 2556936-13 1989 These experiments suggest that although the carbachol-induced increase in Isc is dependent on the increase in [Ca]i, the hormone may activate a second process that increases the sensitivity of the calcium-activated transport process to changes in [Ca]i. Carbachol 44-53 carbonic anhydrase 1 Homo sapiens 248-252 2514612-8 1989 Specific FITC-alpha-BGT binding to the nAChR protein on the optic fibers was inhibited by agonists (e.g., acetylcholine, nicotine, and carbamylcholine) and antagonists (e.g., pancuronium and d-tubocurarine) of the nAChR and was insensitive to high salt concentrations (e.g., 154 mM NaCl). Carbachol 135-150 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 39-44 2531983-6 1989 Intravenous administration of VIP and carbachol significantly stimulated bicarbonate outputs, and these responses were blocked by the VIP antagonist and atropine, respectively. Carbachol 38-47 vasoactive intestinal peptide Rattus norvegicus 134-137 2531983-9 1989 The actions of peripheral VIP and carbachol appear to be mediated by specific VIP and muscarinic receptors, respectively. Carbachol 34-43 vasoactive intestinal peptide Rattus norvegicus 78-81 2482794-6 1989 The data indicate that the amount of Ca2+ mobilized by the muscarinic agonist carbachol or by cholecystokinin octapeptide increases with increasing thyroid hormone concentrations. Carbachol 78-87 carbonic anhydrase 2 Rattus norvegicus 37-40 2596581-7 1989 Stimulation with carbachol resulted in progressive increases in the generation of inositol phosphates and rapid four- to fivefold increases in [Ca2+]i. Carbachol 17-26 carbonic anhydrase 2 Anas platyrhynchos 144-147 2551763-2 1989 This study provides evidence suggesting that, in chronic gastric fistula rats, intracerebroventricularly administered NT (15-60 micrograms) significantly reduces both basal and pentagastrin-, 2-deoxy-D-glucose-, and carbachol-but not histamine-stimulated gastric acid secretion. Carbachol 216-225 neurotensin Rattus norvegicus 118-120 2554897-3 1989 ANF inhibited, in a dose-dependent manner, cholinergic twitch contractions (EC50 = 4.2 nM), nonadrenergic, noncholinergic (NANC) primary and rebound contractions and histamine-induced sustained tonic contraction (but not carbachol induced contraction) of the longitudinal muscle. Carbachol 221-230 natriuretic peptide A Rattus norvegicus 0-3 2482655-9 1989 When carbachol was given 20 s after the injection of VIP or CGRP, the secretory response was increased by about 250% and 60% respectively. Carbachol 5-14 vasoactive intestinal peptide Rattus norvegicus 53-56 2482655-0 1989 The enhancement of carbachol-induced salivary secretion by VIP and CGRP in rat parotid gland is mimicked by forskolin. Carbachol 19-28 vasoactive intestinal peptide Rattus norvegicus 59-62 2482655-0 1989 The enhancement of carbachol-induced salivary secretion by VIP and CGRP in rat parotid gland is mimicked by forskolin. Carbachol 19-28 calcitonin-related polypeptide alpha Rattus norvegicus 67-71 2482655-9 1989 When carbachol was given 20 s after the injection of VIP or CGRP, the secretory response was increased by about 250% and 60% respectively. Carbachol 5-14 calcitonin-related polypeptide alpha Rattus norvegicus 60-64 2795474-6 1989 Concentration-response curves for carbachol-stimulated [3H]inositol monophosphate [( 3H]IP1) accumulation were also obtained. Carbachol 34-43 huntingtin interacting protein 1 Mus musculus 86-91 2804646-2 1989 injections (10 microliters) of carbachol (1.4 nmol), angiotensin II (AII; 48.2 pmol) or a hypertonic solution (990 mOsm/kg) produced increases of plasma vasopressin (AVP) and arterial pressure. Carbachol 31-40 arginine vasopressin Rattus norvegicus 153-164 2551183-1 1989 When dispersed acini from guinea pig pancreas are first incubated with carbachol, the subsequent binding of 125I-vasoactive intestinal peptide (VIP) is inhibited during a second incubation. Carbachol 71-80 VIP peptides Cavia porcellus 108-142 2551183-1 1989 When dispersed acini from guinea pig pancreas are first incubated with carbachol, the subsequent binding of 125I-vasoactive intestinal peptide (VIP) is inhibited during a second incubation. Carbachol 71-80 VIP peptides Cavia porcellus 144-147 2551183-2 1989 This inhibitory action of carbachol on binding of 125I-VIP depends on time, temperature, and the concentration of carbachol in the first incubation and can be blocked by atropine. Carbachol 26-35 VIP peptides Cavia porcellus 55-58 2551183-2 1989 This inhibitory action of carbachol on binding of 125I-VIP depends on time, temperature, and the concentration of carbachol in the first incubation and can be blocked by atropine. Carbachol 114-123 VIP peptides Cavia porcellus 55-58 2551183-4 1989 Adding EGTA to the first incubation medium abolishes the effect of carbachol on binding of 125I-VIP. Carbachol 67-76 VIP peptides Cavia porcellus 96-99 2551183-5 1989 In control acini or acini first incubated with carbachol, approximately half of the bound 125I-VIP can be stripped by acetic acid. Carbachol 47-56 VIP peptides Cavia porcellus 95-98 2551183-10 1989 The dose-response curve for carbachol-induced inhibition of binding of 125I-VIP and that for occupation of low-affinity muscarinic cholinergic receptors by carbachol are similar. Carbachol 28-37 VIP peptides Cavia porcellus 76-79 2550013-7 1989 Vasoactive intestinal polypeptide (VIP) caused a relaxation of smooth muscle cells maximally contracted by carbachol or CCK-8 or gastrin (EC50 = 2.2 nM) with a parallel increase in intracellular cAMP content. Carbachol 107-116 VIP peptides Oryctolagus cuniculus 0-33 2550013-7 1989 Vasoactive intestinal polypeptide (VIP) caused a relaxation of smooth muscle cells maximally contracted by carbachol or CCK-8 or gastrin (EC50 = 2.2 nM) with a parallel increase in intracellular cAMP content. Carbachol 107-116 VIP peptides Oryctolagus cuniculus 35-38 2572704-5 1989 Carbachol induced accumulation of mRNA for c-fos and the other TIS genes. Carbachol 0-9 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 43-48 2554490-3 1989 Pretreatment of T84 cell layers with IFN-gamma for 24 h (but not for 3 h) markedly decreased the Cl- secretory response to vaso-active intestinal polypeptide (VIP) and to cholera toxin and carbachol without appreciably affecting the overall morphology, electrical resistance, or cyclic AMP response of the T84 cell monolayer. Carbachol 189-198 interferon gamma Homo sapiens 37-46 2548215-9 1989 The depolarizing agents choline (EC50 = 1 mM) and carbamoylcholine (EC50 = 1 microM), acting through nicotinic cholinergic receptors, also rescued NGF-deprived neurons. Carbachol 50-66 nerve growth factor Homo sapiens 147-150 2546748-7 1989 Moreover, naloxone (0.5 mg/kg) significantly increased AVP secretion induced by intracerebroventricular injection of carbachol (10 ng). Carbachol 117-126 arginine vasopressin Rattus norvegicus 55-58 2621591-3 1989 In all impaled CA3 pyramidal neurones, continuous applications of carbachol, a non-hydrolysable cholinergic agonist, induced first a brief non-rhythmic excitation and then periodic bursts of RSA which could persist for several hours. Carbachol 66-75 carbonic anhydrase 3 Rattus norvegicus 15-18 2621591-8 1989 Analyses of simultaneous recordings from pairs of neurones, or a neurone and a glial cell, or a neurone and the extracellular field, indicated that carbachol-induced RSA was synchronous in a large population of CA3 pyramidal neurones. Carbachol 148-157 carbonic anhydrase 3 Rattus norvegicus 211-214 2621591-15 1989 Raising extracellular [Ca2+] beyond 7 mM, which should significantly weaken the polysynaptic recurrent excitation among CA3 pyramidal neurones, abolished carbachol-induced RSA. Carbachol 154-163 carbonic anhydrase 2 Rattus norvegicus 23-26 2621591-15 1989 Raising extracellular [Ca2+] beyond 7 mM, which should significantly weaken the polysynaptic recurrent excitation among CA3 pyramidal neurones, abolished carbachol-induced RSA. Carbachol 154-163 carbonic anhydrase 3 Rattus norvegicus 120-123 2621591-16 1989 This suggests that the recurrent excitation among CA3 pyramidal neurones is necessary for carbachol-induced RSA in the CA3 area. Carbachol 90-99 carbonic anhydrase 3 Rattus norvegicus 50-53 2621591-16 1989 This suggests that the recurrent excitation among CA3 pyramidal neurones is necessary for carbachol-induced RSA in the CA3 area. Carbachol 90-99 carbonic anhydrase 3 Rattus norvegicus 119-122 2502025-4 1989 Carbachol increased phosphorylation of a 36-kDa, pI approximately 7 protein (pp36) and transient phosphorylation of a 28-kDa, pI approximately 5 protein (pp28), whereas histamine increased phosphorylation of 40-kDa, pI approximately 6.5 (pp40) and 27-kDa, pI approximately 6.2 (pp27) proteins. Carbachol 0-9 linker for activation of T cells Homo sapiens 77-81 2502025-7 1989 Chelation of [Ca2+]i and [Ca2+]o blocked carbachol-induced phosphorylation of pp28 and pp36 and ionomycin phosphorylation of pp28 but not TPA-stimulated phosphorylation of pp36 or the two pp66s or histamine-stimulated phosphorylation of pp27 or pp40. Carbachol 41-50 linker for activation of T cells Homo sapiens 87-91 2502025-7 1989 Chelation of [Ca2+]i and [Ca2+]o blocked carbachol-induced phosphorylation of pp28 and pp36 and ionomycin phosphorylation of pp28 but not TPA-stimulated phosphorylation of pp36 or the two pp66s or histamine-stimulated phosphorylation of pp27 or pp40. Carbachol 41-50 linker for activation of T cells Homo sapiens 172-176 2502025-10 1989 These phosphoproteins could be common elements of Ca2+-dependent stimulus-secretion coupling as similar proteins were phosphorylated by carbachol and cholecystokinin (CCK) in chief cells. Carbachol 136-145 cholecystokinin Homo sapiens 167-170 2478116-7 1989 Stimulation of monocyte C1-inh synthesis occurs after the addition of agents which induce the formation of sodium ion and calcium ion channels, activate the phosphatidyl inositol cycle and activate protein kinase C (immune-complexes, carbamylcholine and phenylephrine). Carbachol 234-249 serpin family G member 1 Homo sapiens 24-30 2519894-8 1989 Quinacrine, the inhibitor of phospholipase A2, completely inhibits carbachol- and guanine nucleotide-activated AA release and greatly (by about 60-70%) decreases Ca(2+)-dependent AA liberation from phosphatidylinositol. Carbachol 67-76 phospholipase A2 group IB Homo sapiens 29-45 2519894-9 1989 These results indicate that GTP-binding protein(s) are involved in the regulation of carbachol-mediated AA release. Carbachol 85-94 hydroxycarboxylic acid receptor 3 Homo sapiens 28-47 2723657-1 1989 We have examined the effects of the muscarinic agonist carbachol on the intracellular free Ca2+ concentration ([Ca2+]i) in primary cultures of neurons from rat forebrain using the Ca2+-sensitive fluorescent dye fura-2. Carbachol 55-64 carbonic anhydrase 2 Rattus norvegicus 91-94 2723657-1 1989 We have examined the effects of the muscarinic agonist carbachol on the intracellular free Ca2+ concentration ([Ca2+]i) in primary cultures of neurons from rat forebrain using the Ca2+-sensitive fluorescent dye fura-2. Carbachol 55-64 carbonic anhydrase 2 Rattus norvegicus 112-115 2723657-1 1989 We have examined the effects of the muscarinic agonist carbachol on the intracellular free Ca2+ concentration ([Ca2+]i) in primary cultures of neurons from rat forebrain using the Ca2+-sensitive fluorescent dye fura-2. Carbachol 55-64 carbonic anhydrase 2 Rattus norvegicus 112-115 2723657-2 1989 Addition of carbachol increased the [Ca2+]i in approximately 60% of cells studied. Carbachol 12-21 carbonic anhydrase 2 Rattus norvegicus 37-40 2758328-1 1989 Long-term potentiation (LTP) in the CA3 hippocampal subfield, elicited in vivo, produced significant increases in basal and in carbachol- and noradrenaline-induced hydrolysis of phosphatidylinositol-4,5-biphosphate (PtdIns(4,5)P2) as measured by the accumulation of InsP1 in hippocampal slices in vitro. Carbachol 127-136 carbonic anhydrase 3 Homo sapiens 36-39 2527757-2 1989 ANP at concentrations greater than 10(-9) M inhibited carbachol-induced EDRF release from rabbit aorta and acetylcholine-induced EDRF release from the central ear artery. Carbachol 54-63 natriuretic peptides A Oryctolagus cuniculus 0-3 2498332-2 1989 Signal transduction by thyrotropin-releasing hormone (TRH) and carbamylcholine (CCH) in some cells is mediated by inositol lipid hydrolysis forming the second messengers, inositol 1,4,5-trisphosphate (I-1,4,5-P3) and 1,2-diacylglycerol, and causing elevation of cytoplasmic free Ca2+ concentration [( Ca2+]i). Carbachol 80-83 thyrotropin releasing hormone Mus musculus 23-52 2498332-4 1989 TRH, at maximally effective doses, stimulated a rapid marked increase in I-1,4,5-P3 which was associated with a rapid elevation of [Ca2+]i to approximately 1000 nM, whereas maximally effective doses of CCH caused little increase in I-1,4,5-P3 and no burst elevation of [Ca2+]i. Carbachol 202-205 thyrotropin releasing hormone Mus musculus 0-3 2498332-6 1989 CCH-like responses were observed when TtT cells were stimulated by low doses of TRH. Carbachol 0-3 thyrotropin releasing hormone Mus musculus 80-83 2498332-8 1989 Lastly, CCH-like responses were observed with maximally effective doses of TRH when the TRH receptor number was down-regulated to a level similar to that of muscarinic receptors. Carbachol 8-11 thyrotropin releasing hormone Mus musculus 75-78 2498332-8 1989 Lastly, CCH-like responses were observed with maximally effective doses of TRH when the TRH receptor number was down-regulated to a level similar to that of muscarinic receptors. Carbachol 8-11 thyrotropin releasing hormone Mus musculus 88-91 2667680-11 1989 Adenosine selectively reduced the sensitivity of CA1 neurones to microiontophoretically applied carbachol whereas stable nucleotides did not. Carbachol 96-105 carbonic anhydrase 1 Rattus norvegicus 49-52 2758227-7 1989 Carbachol induced a marked Ca2+-dependent increase in the release of acetylcholinesterase but had no effect on the release of butyrylcholinesterase or lactate dehydrogenase. Carbachol 0-9 acetylcholinesterase Cavia porcellus 69-89 2758227-8 1989 This carbachol-evoked increase in acetylcholinesterase release was blocked by hexamethonium but not by atropine. Carbachol 5-14 acetylcholinesterase Cavia porcellus 34-54 2475163-12 1989 The spectroscopic properties of the fluorescent probe ethidium have been used to investigate the effect of the mAbs on the interaction of the agonist carbamylcholine with nAChR in membranes. Carbachol 150-165 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 171-176 2707169-0 1989 The glucocorticoid hormone dexamethasone reverses the growth hormone-releasing properties of the cholinomimetic carbachol. Carbachol 112-121 gonadotropin releasing hormone receptor Rattus norvegicus 54-68 2753222-0 1989 Stimulation and inhibition of prolactin release from rat pituitary lactotrophs by the cholinomimetic carbachol in vitro. Carbachol 101-110 prolactin Rattus norvegicus 30-39 2753222-2 1989 The prolactin (PRL) response of perifused rat pituitary tissue to the cholinomimetic agent carbachol (CARB) was studied under various in vitro conditions. Carbachol 91-100 prolactin Rattus norvegicus 4-13 2753222-2 1989 The prolactin (PRL) response of perifused rat pituitary tissue to the cholinomimetic agent carbachol (CARB) was studied under various in vitro conditions. Carbachol 91-100 prolactin Rattus norvegicus 15-18 2753222-2 1989 The prolactin (PRL) response of perifused rat pituitary tissue to the cholinomimetic agent carbachol (CARB) was studied under various in vitro conditions. Carbachol 102-106 prolactin Rattus norvegicus 4-13 2753222-4 1989 When established in organ culture for 3 days in a serum-free chemically defined medium, there was a significant increase of PRL release in response to CARB. Carbachol 151-155 prolactin Rattus norvegicus 124-127 2753222-5 1989 This PRL releasing activity of CARB depended on the hormonal environment of the culture medium: supplementation of the culture medium with triiodothyronine (T3) and the glucocorticoid dexamethasone (DEX) completely reversed the PRL releasing activity of CARB into an inhibition of PRL release. Carbachol 31-35 prolactin Rattus norvegicus 5-8 2753222-5 1989 This PRL releasing activity of CARB depended on the hormonal environment of the culture medium: supplementation of the culture medium with triiodothyronine (T3) and the glucocorticoid dexamethasone (DEX) completely reversed the PRL releasing activity of CARB into an inhibition of PRL release. Carbachol 31-35 prolactin Rattus norvegicus 228-231 2753222-5 1989 This PRL releasing activity of CARB depended on the hormonal environment of the culture medium: supplementation of the culture medium with triiodothyronine (T3) and the glucocorticoid dexamethasone (DEX) completely reversed the PRL releasing activity of CARB into an inhibition of PRL release. Carbachol 31-35 prolactin Rattus norvegicus 228-231 2539375-3 1989 Stimulation of these cells with serotonin, histamine, carbachol, or the Ca2+ ionophore, ionomycin, increases free cytosolic Ca2+ concentrations and the extent of myosin light chain phosphorylation. Carbachol 54-63 myosin heavy chain 14 Homo sapiens 162-168 2541684-6 1989 PtdIns3P is metabolically closely related to the pool(s) of inositol phospholipid(s) that serves as substrate(s) for an agonist-sensitive phosphoinositidase C, as the levels of PtdIns3P fell significantly when 1321 N1 cells were stimulated with carbachol. Carbachol 245-254 phospholipase C beta 1 Homo sapiens 138-158 2748539-1 1989 The antinociceptive effect of intrathecally administered carbachol at the L1/L2 level in the rat was evaluated using the tail immersion test. Carbachol 57-66 ribosomal protein L4 Rattus norvegicus 74-79 2737706-4 1989 Intravenous vasopressin antagonist, d(CH2)5Tyr(Me) arginine vasopressin, significantly attenuated the pressor response and eliminated the bradycardic response to carbachol in Long-Evans rats. Carbachol 162-171 arginine vasopressin Rattus norvegicus 12-23 2577684-3 1989 Both glucose and theophylline stimulation gave significant increases in somatostatin secretion, whereas carbachol inhibits the somatostatin secretion at 25 mmol l-1 glucose but not at 5 mmol l-1 glucose. Carbachol 104-113 somatostatin Rattus norvegicus 127-139 2466407-2 1989 Atrial natriuretic factor (ANF), atriopeptin II, and atriopeptin III were found to induce relaxation of tracheal smooth muscle precontracted with 5 x 10(-8) M carbachol (an approximate median effective concentration) in a concentration-dependent manner. Carbachol 159-168 natriuretic peptide A Bos taurus 0-25 2466407-2 1989 Atrial natriuretic factor (ANF), atriopeptin II, and atriopeptin III were found to induce relaxation of tracheal smooth muscle precontracted with 5 x 10(-8) M carbachol (an approximate median effective concentration) in a concentration-dependent manner. Carbachol 159-168 natriuretic peptide A Bos taurus 27-30 2466407-5 1989 However, the relaxant effects of ANF, atriopeptin II, and atriopeptin III were much less when a higher concentration of carbachol or 30 mM K+ was used as the contractile agent. Carbachol 120-129 natriuretic peptide A Bos taurus 33-36 2466407-6 1989 Maximally inhibitory concentration (IC50) values of ANF, atriopeptin II, and atriopeptin III for inhibition of muscle contraction induced by 5 x 10(-8) M carbachol ranged from 3.8 to 8.3 x 10(-9) M, indicating that these peptides have intermediate potency between isoproterenol (IC50, 2.0 x 10(-9) M) and sodium nitroprusside (IC50, 2.0 x 10(-8) M). Carbachol 154-163 natriuretic peptide A Bos taurus 52-55 2466407-7 1989 Treatment of the muscle with 3 x 10(-7) M ANF slowed the rate of tension development of the muscle by 10(-7) M carbachol. Carbachol 111-120 natriuretic peptide A Bos taurus 42-45 2466411-3 1989 Hybridization to dot blots of total parietal cell RNA showed a significant increase in specific CA II mRNA content within minutes of secretagogue addition: carbachol stimulation led to an increase of 52 +/- 8.9%, reaching a maximum within 20 min; gastrin stimulation led to an increase within 60 min of 104 +/- 10.6%; stimulation with histamine was followed within 20 min by an increase of 30 +/- 7.2%. Carbachol 156-165 gastrin Canis lupus familiaris 247-254 2737706-4 1989 Intravenous vasopressin antagonist, d(CH2)5Tyr(Me) arginine vasopressin, significantly attenuated the pressor response and eliminated the bradycardic response to carbachol in Long-Evans rats. Carbachol 162-171 arginine vasopressin Rattus norvegicus 60-71 2737706-7 1989 Combined intravenous treatment with phentolamine and vasopressin antagonist completely eliminated the pressor response to carbachol in Long-Evans rats. Carbachol 122-131 arginine vasopressin Rattus norvegicus 53-64 2737706-10 1989 Hypertensive response to intracerebroventricularly administered carbachol in normal rats is mediated both by an increase in central sympathetic outflow and in circulating vasopressin. Carbachol 64-73 arginine vasopressin Rattus norvegicus 171-182 2566191-3 1989 Somatostatin inhibited ion secretion evoked by EFS (55-65%), carbachol (80%) and VIP (95%) in a dose-dependent manner. Carbachol 61-70 somatostatin Rattus norvegicus 0-12 2566191-6 1989 In contrast, vasopressin in concentrations as low as 0.025-0.25 U/liter decreased the secretory effects of EFS (55-75%) and carbachol (85%), but had no effect on cAMP-dependent secretion elicited either by VIP or forskolin. Carbachol 124-133 arginine vasopressin Rattus norvegicus 13-24 2538074-5 1989 In conditions where carbachol stimulated mucin secretion from filter-grown Cl.16E cells, VIP, dibutyryl adenosine 3",5"-cyclic monophosphate (DbcAMP), or forskolin did not alter basal secretion. Carbachol 20-29 LOC100508689 Homo sapiens 41-46 2538074-6 1989 However, VIP strongly potentiated carbachol-induced mucin secretion, since carbachol alone and VIP plus carbachol induced a 1.6- and 3.6-fold increase of mucin secretion above basal, respectively. Carbachol 34-43 vasoactive intestinal peptide Homo sapiens 9-12 2538074-6 1989 However, VIP strongly potentiated carbachol-induced mucin secretion, since carbachol alone and VIP plus carbachol induced a 1.6- and 3.6-fold increase of mucin secretion above basal, respectively. Carbachol 34-43 LOC100508689 Homo sapiens 52-57 2538074-6 1989 However, VIP strongly potentiated carbachol-induced mucin secretion, since carbachol alone and VIP plus carbachol induced a 1.6- and 3.6-fold increase of mucin secretion above basal, respectively. Carbachol 34-43 vasoactive intestinal peptide Homo sapiens 95-98 2538074-6 1989 However, VIP strongly potentiated carbachol-induced mucin secretion, since carbachol alone and VIP plus carbachol induced a 1.6- and 3.6-fold increase of mucin secretion above basal, respectively. Carbachol 34-43 LOC100508689 Homo sapiens 154-159 2538074-6 1989 However, VIP strongly potentiated carbachol-induced mucin secretion, since carbachol alone and VIP plus carbachol induced a 1.6- and 3.6-fold increase of mucin secretion above basal, respectively. Carbachol 75-84 LOC100508689 Homo sapiens 154-159 2538074-6 1989 However, VIP strongly potentiated carbachol-induced mucin secretion, since carbachol alone and VIP plus carbachol induced a 1.6- and 3.6-fold increase of mucin secretion above basal, respectively. Carbachol 75-84 LOC100508689 Homo sapiens 154-159 2783692-8 1989 The Ca2+ ionophore ionomycin, and two other Ca2+-mobilizing hormones, bradykinin and histamine, mimic the effects of carbachol. Carbachol 117-126 kininogen 1 Homo sapiens 70-80 2755876-4 1989 The neuropeptide function of neuromedin C and/or other BLI peptides at this level was supported by the stimulatory effect of carbamylcholine (500 microM) on the release of BLI (4.5-fold increase over the basal release of 19 fmol/5 min) from perfused bovine adrenal glands. Carbachol 125-140 gastrin releasing peptide Homo sapiens 29-41 2919678-1 1989 In previous studies we demonstrated that parietal cell stimulation with gastrin and carbamoylcholine (carbachol) is accompanied by increased turnover of membrane inositol phospholipids. Carbachol 102-111 gastrin Homo sapiens 72-79 2531022-0 1989 [TRH dissociates the aggressivity of vegetative and motor phenomena induced by carbachol in the cat]. Carbachol 79-88 thyrotropin releasing hormone Felis catus 1-4 2805565-5 1989 The naphthalene sulfonamides W7 and W5 which bind calmodulin and thus block the intracellular transduction of Ca2+ effects also inhibited a carbachol-induced H+ production. Carbachol 140-149 calmodulin 1 Rattus norvegicus 50-60 2561651-4 1989 Carbachol-induced a stimulation of the PPI turnover cycle: namely, a decrease in 32Pi incorporation into phosphatidylinositol-4,5-bisphosphate (TPI) and phosphatidylinositol-4-phosphate (DPI), and an increase in this incorporation into phosphatidylinositol (PI) and phosphatidic acid (PA) in rat brain synaptosomes from the cerebrum. Carbachol 0-9 triosephosphate isomerase 1 Rattus norvegicus 144-147 2558909-5 1989 Preliminary experiments on CA1 neurons in hippocampal slices (by single electrode voltage clamp), confirmed previous reports that carbachol depresses A- and C-type K currents, as well as inward Ca2+ currents; though the latter effect was sometimes mainly due to frequency-dependent inactivation of Ca currents. Carbachol 130-139 carbonic anhydrase 1 Rattus norvegicus 27-30 2474153-1 1989 CCK-8-induced desensitization of carbachol-stimulated amylase secretion occurred over a range of concentrations of CCK-8 that occupy low affinity CCK receptors. Carbachol 33-42 cholecystokinin Homo sapiens 0-3 2474153-1 1989 CCK-8-induced desensitization of carbachol-stimulated amylase secretion occurred over a range of concentrations of CCK-8 that occupy low affinity CCK receptors. Carbachol 33-42 cholecystokinin Homo sapiens 115-118 2474153-1 1989 CCK-8-induced desensitization of carbachol-stimulated amylase secretion occurred over a range of concentrations of CCK-8 that occupy low affinity CCK receptors. Carbachol 33-42 cholecystokinin Homo sapiens 115-118 2474153-3 1989 Analysis of the relationship between receptor occupation and CCK-8-induced desensitization caused by CCK-8 and CCK-JMV-180 in combination also indicated that CCK-8-induced desensitization of carbachol-stimulated amylase secretion is caused by occupation of low affinity CCK receptors. Carbachol 191-200 cholecystokinin Homo sapiens 61-64 2474153-3 1989 Analysis of the relationship between receptor occupation and CCK-8-induced desensitization caused by CCK-8 and CCK-JMV-180 in combination also indicated that CCK-8-induced desensitization of carbachol-stimulated amylase secretion is caused by occupation of low affinity CCK receptors. Carbachol 191-200 cholecystokinin Homo sapiens 101-104 2474153-3 1989 Analysis of the relationship between receptor occupation and CCK-8-induced desensitization caused by CCK-8 and CCK-JMV-180 in combination also indicated that CCK-8-induced desensitization of carbachol-stimulated amylase secretion is caused by occupation of low affinity CCK receptors. Carbachol 191-200 cholecystokinin Homo sapiens 101-104 2474153-3 1989 Analysis of the relationship between receptor occupation and CCK-8-induced desensitization caused by CCK-8 and CCK-JMV-180 in combination also indicated that CCK-8-induced desensitization of carbachol-stimulated amylase secretion is caused by occupation of low affinity CCK receptors. Carbachol 191-200 cholecystokinin Homo sapiens 101-104 2474153-3 1989 Analysis of the relationship between receptor occupation and CCK-8-induced desensitization caused by CCK-8 and CCK-JMV-180 in combination also indicated that CCK-8-induced desensitization of carbachol-stimulated amylase secretion is caused by occupation of low affinity CCK receptors. Carbachol 191-200 cholecystokinin Homo sapiens 101-104 2575762-2 1989 Carvedilol produced competitive antagonism of the beta 1-adrenoceptor mediated positive chronotropic response to isoproterenol in guinea pig atria, and the beta 2-adrenoceptor mediated relaxation to isoproterenol in carbachol (1 mumol/l) precontracted guinea pig trachea, with a dissociation constant (KB) for beta 1-adrenoceptors of 0.8 nmol/l and beta 2-adrenoceptors of 1.3 nmol/l. Carbachol 216-225 beta-2 adrenergic receptor Cavia porcellus 156-175 2464057-1 1989 The new calmodulin antagonist, CGS-9343B, was found to inhibit both histamine plus 3-isobutyl-1-methylxanthine and carbachol-induced [14C]aminopyrine accumulation in dispersed, fundic mucosal cells of rats. Carbachol 115-124 calmodulin 1 Rattus norvegicus 8-18 2660131-7 1989 In contrast, insulin secretion stimulated by glucose or the cholinergic agonist carbachol was inhibited by PYY (by 33 and 26%, respectively, at 4.25 nmol/kg). Carbachol 80-89 peptide YY Mus musculus 107-110 2660131-8 1989 Similarly, carbachol-induced glucagon secretion was inhibited by PYY (by 47% at 4.25 nmol/kg). Carbachol 11-20 peptide YY Mus musculus 65-68 2666982-8 1989 Secretion of PP by SL and UP cultures in response to 5 microM carbachol and 2.8 mM glucose in a 2-h incubation was significantly greater, 2.2- (p less than 0.002) and 3.9-fold (p less than 0.002), respectively, than secretion by respective control cultures without carbachol. Carbachol 62-71 pancreatic polypeptide Canis lupus familiaris 13-15 2666982-8 1989 Secretion of PP by SL and UP cultures in response to 5 microM carbachol and 2.8 mM glucose in a 2-h incubation was significantly greater, 2.2- (p less than 0.002) and 3.9-fold (p less than 0.002), respectively, than secretion by respective control cultures without carbachol. Carbachol 265-274 pancreatic polypeptide Canis lupus familiaris 13-15 2848833-3 1988 Muscarinic stimulation (carbachol) of the acini produced a gradual rise in pHi (approximately 0.1 unit by 10 min) possibly due to activation of the Na+/H+ exchanger. Carbachol 24-33 glucose-6-phosphate isomerase Rattus norvegicus 75-78 2848833-4 1988 When the exchanger was blocked by amiloride or sodium removal, carbachol induced a dramatic (atropine inhibitable) decrease in pHi (approximately 0.4 pH unit with t1/2 approximately 0.5 min at 1 mM carbachol). Carbachol 63-72 glucose-6-phosphate isomerase Rattus norvegicus 127-130 2848833-4 1988 When the exchanger was blocked by amiloride or sodium removal, carbachol induced a dramatic (atropine inhibitable) decrease in pHi (approximately 0.4 pH unit with t1/2 approximately 0.5 min at 1 mM carbachol). Carbachol 198-207 glucose-6-phosphate isomerase Rattus norvegicus 127-130 3075611-7 1988 Carbachol raised plasma gastrin immunoreactivity; but in no instance was there a significant relationship between gastric secretion and plasma gastrin immunoreactivity. Carbachol 0-9 gastrin Canis lupus familiaris 24-31 3075611-10 1988 PP plasma immunoreactivity was elevated by methacholine and carbachol. Carbachol 60-69 pancreatic polypeptide Canis lupus familiaris 0-2 2783255-2 1989 Both NGF and EGF potentiate in these cells the increase in the accumulation of inositol phosphates that is elicited by bradykinin and carbachol. Carbachol 134-143 nerve growth factor Rattus norvegicus 5-8 2783255-2 1989 Both NGF and EGF potentiate in these cells the increase in the accumulation of inositol phosphates that is elicited by bradykinin and carbachol. Carbachol 134-143 epidermal growth factor like 1 Rattus norvegicus 13-16 2847543-3 1988 Cultured cells also showed the ability to secrete immunoreactive gastrin, and this secretion was further concentration-dependently stimulated by secretin (10(-10)-10(-6) M), carbachol (10(-6) M), and bombesin (10(-10)-10(-6) M). Carbachol 174-183 gastrin Homo sapiens 65-72 2847543-3 1988 Cultured cells also showed the ability to secrete immunoreactive gastrin, and this secretion was further concentration-dependently stimulated by secretin (10(-10)-10(-6) M), carbachol (10(-6) M), and bombesin (10(-10)-10(-6) M). Carbachol 174-183 gastrin releasing peptide Homo sapiens 200-208 3237319-2 1988 An intraventricular injection of hypertonic saline, carbachol, angiotensin II, prostaglandin E2 or histamine to a rabbit increased the concentrations of plasma AVP and OXT, whereas serotonin decreased their plasma levels. Carbachol 52-61 vasopressin-neurophysin 2-copeptin Oryctolagus cuniculus 160-163 2846548-13 1988 These results show (a) that the beta 2-adrenoceptor couples in isolated tracheal smooth muscle cells to a dihydropyridine- and pertussis toxin-sensitive calcium channel; (b) that the same channel is opened by carbachol; (c) that cGMP kinase is very effective in decreasing elevated cytosolic calcium concentrations, whereas cAMP-dependent protein kinase has a variable effect on stimulated cytosolic calcium levels. Carbachol 209-218 adrenoceptor beta 2 Bos taurus 32-51 2464161-5 1988 While all these agents cause chloride secretion by elevating intracellular cAMP, NPY is also effective in inhibiting the secretory effects of carbachol (CCh) and substance P (SP), agents believed to act by raising intracellular calcium (Cai). Carbachol 142-151 neuropeptide Y Rattus norvegicus 81-84 2464161-5 1988 While all these agents cause chloride secretion by elevating intracellular cAMP, NPY is also effective in inhibiting the secretory effects of carbachol (CCh) and substance P (SP), agents believed to act by raising intracellular calcium (Cai). Carbachol 153-156 neuropeptide Y Rattus norvegicus 81-84 3170551-6 1988 Carbachol (10 microM) or ionomycin (10 microM) stimulation of fura-2-containing cells resulted in a rapid increase in cytosolic free Ca2+ concentration and in the extent of myosin light chain phosphorylation. Carbachol 0-9 myosin heavy chain 14 Homo sapiens 173-179 3170551-10 1988 There was a similar relationship between free cytosolic Ca2+ concentrations and the extent of myosin light chain phosphorylation in carbachol- and ionomycin-stimulated cells. Carbachol 132-141 myosin heavy chain 14 Homo sapiens 94-100 3237319-2 1988 An intraventricular injection of hypertonic saline, carbachol, angiotensin II, prostaglandin E2 or histamine to a rabbit increased the concentrations of plasma AVP and OXT, whereas serotonin decreased their plasma levels. Carbachol 52-61 oxytocin Oryctolagus cuniculus 168-171 3148968-3 1988 Infusion of gastrin-releasing peptide at 10(-8) M significantly increased pepsin output (from 87 +/- 17 to 129 +/- 22 units pepsin/min) and simultaneous infusion of gastrin-releasing peptide and carbachol at 10(-8) and 10(-6) M, respectively, resulted in an increase to almost 4 times the basal values. Carbachol 195-204 gastrin releasing peptide Rattus norvegicus 12-37 2843365-14 1988 Inhibition was partly improved (60% inhibition at 1 microM carbachol) when the contribution of the Gs-C species was decreased by lowering the concentration of local PGI2. Carbachol 59-68 goosecoid homeobox Homo sapiens 99-103 3417778-6 1988 Exposure of chick myotubes to the cholinergic agonist carbamylcholine was found to cause a dispersal of AChR clusters with a time course similar to that of TPA. Carbachol 54-69 cholinergic receptor nicotinic delta subunit Gallus gallus 104-108 3417778-7 1988 Like TPA, carbamylcholine enhances the phosphorylation of the delta-subunit of AChR. Carbachol 10-25 cholinergic receptor nicotinic delta subunit Gallus gallus 79-83 3417778-8 1988 The carbamylcholine-induced redistribution and phosphorylation of AChR is blocked by the nicotinic AChR antagonist d-tubocurarine. Carbachol 4-19 cholinergic receptor nicotinic delta subunit Gallus gallus 66-70 3417778-8 1988 The carbamylcholine-induced redistribution and phosphorylation of AChR is blocked by the nicotinic AChR antagonist d-tubocurarine. Carbachol 4-19 cholinergic receptor nicotinic delta subunit Gallus gallus 99-103 2903467-3 1988 Barium and carbachol both inactivate the m-current and these results suggest that somatostatin may exert its hyperpolarizing action on CA1 pyramidal cells by activation of the m-current. Carbachol 11-20 carbonic anhydrase 1 Homo sapiens 135-138 3404446-1 1988 We investigated whether the inhibition of prolactin secretion from pituitary cells by carbachol, a cholinergic agonist resistant to hydrolysis by cholinesterases, would be a useful bioassay to explore an important nonneuronal action of antimuscarinic agents. Carbachol 86-95 prolactin Rattus norvegicus 42-51 3404446-2 1988 Carbachol inhibited prolactin secretion from cultured rat anterior pituitary cells in a dose-dependent manner with a mean IC50 of 1.5 +/- 0.6 (S.E.) Carbachol 0-9 prolactin Rattus norvegicus 20-29 2457366-4 1988 The half-maximal concentration for carbachol-stimulation of cAMP levels (6 microM) was similar to that for the previously determined carbachol-induced stimulation of phosphoinositide turnover in these cells, suggesting that the former is mediated by the latter. Carbachol 35-44 cathelicidin antimicrobial peptide Homo sapiens 60-64 3208090-1 1988 Iontophoretic application of carbachol caused excitation of CA1 neurones and decreased the amplitude of antidromic CA1 population spikes recorded extracellularly. Carbachol 29-38 carbonic anhydrase 1 Rattus norvegicus 60-63 3208090-1 1988 Iontophoretic application of carbachol caused excitation of CA1 neurones and decreased the amplitude of antidromic CA1 population spikes recorded extracellularly. Carbachol 29-38 carbonic anhydrase 1 Rattus norvegicus 115-118 3139031-3 1988 Pretreatment of cultures from both ages by Bordetella pertussis toxin (IAP) was found to eliminate any Gpp(NH)p effect on carbamylcholine binding. Carbachol 122-137 Cd47 molecule Rattus norvegicus 71-74 3139031-4 1988 IAP by itself induced a rightward shift in the carbamylcholine competition curve in homogenates from aged cultures, but no such effect was observed in homogenates from young cultures. Carbachol 47-62 Cd47 molecule Rattus norvegicus 0-3 3140614-3 1988 The ACE inhibitors antagonised adrenaline-, carbachol- and A23187-stimulated PGI2 synthesis in the aorta and bladder (CGS14824A greater than captopril greater than CGS14831) but were without effect on trauma- or arachidonate-stimulated PGI2 synthesis. Carbachol 44-53 angiotensin I converting enzyme Rattus norvegicus 4-7 2841015-5 1988 Pretreatment of cells with IAP prior to carbachol exposure partially blocked the subsequent decrease in receptor number. Carbachol 40-49 magnesium transporter 1 Mus musculus 27-30 2454032-1 1988 Rat calcitonin gene-related peptide (rat CGRP) and related peptides did not cause contraction of gastric smooth muscle cells; however, preincubation with rat CGRP or human CGRP inhibited smooth muscle contraction caused by carbachol. Carbachol 223-232 calcitonin-related polypeptide alpha Rattus norvegicus 158-162 2454032-1 1988 Rat calcitonin gene-related peptide (rat CGRP) and related peptides did not cause contraction of gastric smooth muscle cells; however, preincubation with rat CGRP or human CGRP inhibited smooth muscle contraction caused by carbachol. Carbachol 223-232 calcitonin related polypeptide alpha Homo sapiens 158-162 3395782-0 1988 Positive inotropic effects induced by carbachol in rat atria treated with islet-activating protein (IAP)--association with phosphatidylinositol breakdown. Carbachol 38-47 Cd47 molecule Rattus norvegicus 100-103 3390705-1 1988 Effects of pertussis toxin or cholera toxin on carbachol-stimulated inositol-1-phosphate ([3H]IP1) accumulation were studied using the human neuroblastoma cell line (SH-SY5Y). Carbachol 47-56 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 94-97 3246807-5 1988 VIP (10(-6) M) inhibited spontaneous and carbachol (10(-8) M)-stimulated propulsive activities of the isolated segment in distal colon. Carbachol 41-50 VIP peptides Cavia porcellus 0-3 3404446-3 1988 microM and maximal inhibition at 10(-5) M. Prolactin levels in media were significantly reduced by 30 min of incubation with carbachol. Carbachol 125-134 prolactin Rattus norvegicus 43-52 3404446-5 1988 The stimulation of prolactin secretion by 10(-6) M thyrotropin releasing hormone (to 1.5 times control) was inhibited by the addition of carbachol (10(-5) M). Carbachol 137-146 prolactin Rattus norvegicus 19-28 2456808-4 1988 Compared to CCh, ACh was a full agonist for contraction but AHR-602 and McN-A-343 were partial agonists producing 80-85% of the maximal response to CCh. Carbachol 148-151 aryl hydrocarbon receptor Cavia porcellus 60-63 2456808-5 1988 Similar to previous findings with CCh, tonic contractions produced by AHR-602 and McN-A-343 were less sensitive to inhibition by nifedipine or verapamil than tonic contractions to ACh. Carbachol 34-37 aryl hydrocarbon receptor Cavia porcellus 70-73 2456808-10 1988 A concentration of 0.2 mM AHR-602 produced a parallel shift of the concentration-response curve to CCh on inositol phosphate accumulation. Carbachol 99-102 aryl hydrocarbon receptor Cavia porcellus 26-29 3390705-2 1988 The maximal carbachol-stimulated [3H]IP1 accumulation in the SH-SY5Y cells was decreased from 51.4 fmol/10(6) cells to 42.4 fmol/10(6) cells (P less than 0.05) and 22.1 fmol/10(6) cells (P less than 0.01) in the absence and presence of 1 microgram/ml and 10 micrograms/ml pertussis toxin, respectively while the EC50 values did not change. Carbachol 12-21 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 37-40 3342763-5 1988 Intracerebroventricular administration of leumorphin (600 pmol) also reduced the AVP secretion stimulated by icv injection of carbachol (50 pmol). Carbachol 126-135 arginine vasopressin Rattus norvegicus 81-84 2898507-5 1988 Cholecystokinin (10(-9) M) and carbachol (10(-5) M) efficiently stimulated lipase secretion (3 fold over basal rate). Carbachol 31-40 lipase G, endothelial type Rattus norvegicus 75-81 2453245-12 1988 CCh and substance P (SP) elicited contractions at ED 15 and vasoactive intestinal polypeptide (VIP) caused a relaxation at ED 16. Carbachol 0-3 vasoactive intestinal peptide Rattus norvegicus 60-93 2969821-3 1988 In contrast, ANF markedly and significantly inhibited carbachol-induced NE release in a concentration-dependent manner. Carbachol 54-63 natriuretic peptide A Rattus norvegicus 13-16 3420006-2 1988 Results of these studies indicate that norepinephrine and carbachol evoke pharmacologically and temporally distinctive patterns of antral gastrin release. Carbachol 58-67 gastrin Rattus norvegicus 138-145 3420006-3 1988 Dose-response experiments indicate that norepinephrine is approximately 10,000 times more potent on a molar basis than carbachol in stimulating antral gastrin release. Carbachol 119-128 gastrin Rattus norvegicus 151-158 2840296-0 1988 5-HT1A receptor agonists inhibit carbachol-induced stimulation of phosphoinositide turnover in the rat hippocampus. Carbachol 33-42 5-hydroxytryptamine receptor 1A Rattus norvegicus 0-6 2452098-1 1988 The increased insulin release induced by carbamoylcholine (CbCh) in pancreatic islets requires the presence of extracellular Ca2+. Carbachol 41-57 insulin Homo sapiens 14-21 2452098-1 1988 The increased insulin release induced by carbamoylcholine (CbCh) in pancreatic islets requires the presence of extracellular Ca2+. Carbachol 59-63 insulin Homo sapiens 14-21 2450590-3 1988 However, VIP and 8-bromo-cyclic AMP, when added in combination with carbamylcholine, potentiated the stimulation of amylase release to 170-180% of that caused by carbamylcholine alone. Carbachol 162-177 vasoactive intestinal polypeptide Mus musculus 9-12 2450590-4 1988 As assessed by two-dimensional gel electrophoresis, VIP reproduced four of the ten changes in protein phosphorylation elicited by carbamylcholine, these changes being the increased phosphorylation of one soluble protein and the decreased phosphorylation of three soluble proteins. Carbachol 130-145 vasoactive intestinal polypeptide Mus musculus 52-55 2450590-5 1988 VIP enhanced the carbamylcholine-induced changes in phosphorylation for three proteins. Carbachol 17-32 vasoactive intestinal polypeptide Mus musculus 0-3 2450590-10 1988 Moreover, these effects appear to be mediated primarily by VIP-preferring receptors and may be involved in the synergistic action of VIP to promote carbamylcholine-induced amylase release. Carbachol 148-163 vasoactive intestinal polypeptide Mus musculus 59-62 2450590-10 1988 Moreover, these effects appear to be mediated primarily by VIP-preferring receptors and may be involved in the synergistic action of VIP to promote carbamylcholine-induced amylase release. Carbachol 148-163 vasoactive intestinal polypeptide Mus musculus 133-136 2898270-2 1988 Chlorisondamine, a ganglionic blocking agent, greatly attenuated the induction of TH mRNA levels caused by cold exposure, whereas carbachol and nicotine, cholinergic agonists, increased TH mRNA in control animals. Carbachol 130-139 tyrosine hydroxylase Rattus norvegicus 186-188 2897641-5 1988 Further evidence for the cholinergic regulation of the CRH neuron was provided by the findings that both carbachol, a muscarinic receptor agonist, and nicotine, a nicotinic receptor agonist, stimulated IR-rCRH secretion in a dose-dependent fashion. Carbachol 105-114 corticotropin releasing hormone Rattus norvegicus 55-58 2452220-7 1988 Also, the calmodulin antagonist drug W7 (N-6-(aminohexyl)-5-chloro-1-naphthalene sulfonamide) inhibited the carbamylcholine-induced release of intracellular Ca2+ in acinar carcinoma cells. Carbachol 108-123 calmodulin 1 Rattus norvegicus 10-20 3284870-6 1988 Intravenous histamine or carbachol aerosol had similar effects on sRL and Vtr. Carbachol 25-34 sarcalumenin Ovis aries 66-69 3338643-2 1988 The release of PP from the dorsal part of the rat pancreas by GRP and carbachol was also studied. Carbachol 70-79 pancreatic polypeptide Rattus norvegicus 15-17 3338643-3 1988 Carbachol and GRP stimulated, in a dose-dependent manner ranging from 10(-6) to 10(-9) M, PP secretion from the ventral part of the pancreas. Carbachol 0-9 pancreatic polypeptide Rattus norvegicus 90-92 3338643-8 1988 The potency of GRP and vasoactive intestinal polypeptide relative to carbachol at 10(-7) M was 24% and 7%, respectively. Carbachol 69-78 gastrin releasing peptide Rattus norvegicus 15-18 3359107-0 1988 Central inhibition by gamma-aminobutyric acid of the release of vasopressin by carbachol in the rat. Carbachol 79-88 arginine vasopressin Rattus norvegicus 64-75 2892268-4 1988 Since the muscarinic cholinergic agonists carbachol and muscarine antagonized this current, these results suggest a reciprocal regulation of the M-current by somatostatin and acetylcholine. Carbachol 42-51 somatostatin Homo sapiens 158-170 3359107-2 1988 gamma-Aminobutyric acid (GABA) inhibited the antidiuretic response and the increased urinary excretion of vasopressin produced by carbachol when both drugs were injected into a lateral cerebral ventricle (i.c.v.) Carbachol 130-139 arginine vasopressin Rattus norvegicus 106-117 3359314-1 1988 The development of carbachol-induced EEG theta (theta) activity was studied in the CA1 and dentate regions of hippocampal formation slices obtained from neonatal rats (4, 6, 8, 10, 12 and 14 days of age). Carbachol 19-28 carbonic anhydrase 1 Rattus norvegicus 83-86 3342003-2 1988 The cytoplasmic Ca2+ concentration increased from 237 +/- 6 nM to 1580 +/- 170 nM within 3-5 s of addition of 300 microM-carbachol. Carbachol 121-130 carbonic anhydrase 2 Homo sapiens 16-19 3342003-5 1988 Histamine increased cytoplasmic Ca2+ to about 40% of the maximal value seen with carbachol. Carbachol 81-90 carbonic anhydrase 2 Homo sapiens 32-35 3342003-7 1988 The increase in Ca2+ in response to either carbachol or histamine could be completely inhibited by pretreating the cells with carbachol; the response to carbachol could be partially inhibited by pretreating the cells with histamine. Carbachol 43-52 carbonic anhydrase 2 Homo sapiens 16-19 3342003-7 1988 The increase in Ca2+ in response to either carbachol or histamine could be completely inhibited by pretreating the cells with carbachol; the response to carbachol could be partially inhibited by pretreating the cells with histamine. Carbachol 126-135 carbonic anhydrase 2 Homo sapiens 16-19 3342003-7 1988 The increase in Ca2+ in response to either carbachol or histamine could be completely inhibited by pretreating the cells with carbachol; the response to carbachol could be partially inhibited by pretreating the cells with histamine. Carbachol 126-135 carbonic anhydrase 2 Homo sapiens 16-19 3342003-10 1988 The results indicate that carbachol and histamine stimulate release of Ca2+ from the same intracellular Ca2+ store, that depletion of this store is responsible for heterologous desensitization between these two agonists, and that repletion of the agonist-sensitive Ca2+ pool does not occur in the continued presence of agonist or in the absence of extracellular Ca2+. Carbachol 26-35 carbonic anhydrase 2 Homo sapiens 71-74 3342003-10 1988 The results indicate that carbachol and histamine stimulate release of Ca2+ from the same intracellular Ca2+ store, that depletion of this store is responsible for heterologous desensitization between these two agonists, and that repletion of the agonist-sensitive Ca2+ pool does not occur in the continued presence of agonist or in the absence of extracellular Ca2+. Carbachol 26-35 carbonic anhydrase 2 Homo sapiens 104-107 3342003-10 1988 The results indicate that carbachol and histamine stimulate release of Ca2+ from the same intracellular Ca2+ store, that depletion of this store is responsible for heterologous desensitization between these two agonists, and that repletion of the agonist-sensitive Ca2+ pool does not occur in the continued presence of agonist or in the absence of extracellular Ca2+. Carbachol 26-35 carbonic anhydrase 2 Homo sapiens 104-107 3342003-10 1988 The results indicate that carbachol and histamine stimulate release of Ca2+ from the same intracellular Ca2+ store, that depletion of this store is responsible for heterologous desensitization between these two agonists, and that repletion of the agonist-sensitive Ca2+ pool does not occur in the continued presence of agonist or in the absence of extracellular Ca2+. Carbachol 26-35 carbonic anhydrase 2 Homo sapiens 104-107 3242998-3 1988 Intravenous vasopressin antagonist, d(CH2)5 Tyr(Me)AVP, significantly attenuated the pressor response and eliminated the bradycardic response to carbachol in LE rats. Carbachol 145-154 arginine vasopressin Rattus norvegicus 12-23 3242998-7 1988 carbachol in normal rats is mediated both by an increase in central sympathetic outflow and in circulating vasopressin. Carbachol 0-9 arginine vasopressin Rattus norvegicus 107-118 2450276-4 1988 Upon stimulation with carbamylcholine (CCh) or cholecystokinin (CCK), [Ca2+]i increased within seconds by 4- to 8-fold. Carbachol 22-37 cholecystokinin Rattus norvegicus 64-67 3337221-7 1988 The mechanism of chloride secretion induced by histamine resembled that of carbachol, in that both 1) were associated with an increase in free cytosolic calcium, 2) had a site of activation at a basolaterally localized K+ channel, and 3) were potentiated by both cAMP- and cGMP-mediated secretagogues. Carbachol 75-84 cathelicidin antimicrobial peptide Homo sapiens 263-268 20501312-2 1988 Increases in the level of c-AMP observed following application of forskolin, isoproterenol and VIP are decreased by carbachol in a dose-dependent manner. Carbachol 116-125 antimicrobial protein CAP18 Oryctolagus cuniculus 26-31 20501312-2 1988 Increases in the level of c-AMP observed following application of forskolin, isoproterenol and VIP are decreased by carbachol in a dose-dependent manner. Carbachol 116-125 VIP peptides Oryctolagus cuniculus 95-98 20501312-4 1988 Carbachol attenuation of elevated c-AMP levels can be inhibited by the muscarinic antagonist atropine but not by the specific muscarinic receptor antagonist pirenzepine. Carbachol 0-9 antimicrobial protein CAP18 Oryctolagus cuniculus 34-39 2896351-2 1988 Pretreatment of pancreatic acini at 37 degrees C for 120 min with not only pancreatic secretagogues, such as carbachol and bombesin, but also vasoactive intestinal peptide (VIP) and secretin reduced subsequent labeled somatostatin binding to the acinar membranes in a dose-dependent manner. Carbachol 109-118 somatostatin Rattus norvegicus 218-230 2896351-7 1988 Furthermore, the inhibitory effect of carbachol was attenuated by the presence of 1 mM EDTA in media for pretreatment, suggesting that intracellular pathways activated by pancreatic secretagogues may be responsible for somatostatin receptor modulation. Carbachol 38-47 somatostatin Rattus norvegicus 219-231 2896351-8 1988 Interestingly, when combined with VIP, pretreatment of acini with carbachol produced an additive inhibition of labeled somatostatin binding to the membranes. Carbachol 66-75 vasoactive intestinal peptide Rattus norvegicus 34-37 2896351-8 1988 Interestingly, when combined with VIP, pretreatment of acini with carbachol produced an additive inhibition of labeled somatostatin binding to the membranes. Carbachol 66-75 somatostatin Rattus norvegicus 119-131 3227877-4 1988 Stimulation of vagotomized rats with pentagastrin and carbachol reduced the levels of thioredoxin and thioredoxin reductase. Carbachol 54-63 thioredoxin 1 Rattus norvegicus 86-97 3227877-4 1988 Stimulation of vagotomized rats with pentagastrin and carbachol reduced the levels of thioredoxin and thioredoxin reductase. Carbachol 54-63 peroxiredoxin 5 Rattus norvegicus 102-123 2485249-2 1988 Behavioral analysis of acetylcholine, substance P and corticotropin releasing factor microinjected into the medial anterior cortex revealed a seizure-related "boxing" behavior elicited by carbachol, which was potentiated by coinjection with substance P and antagonized by coinjection with corticotropin releasing factor. Carbachol 188-197 corticotropin releasing hormone Rattus norvegicus 54-84 2485249-2 1988 Behavioral analysis of acetylcholine, substance P and corticotropin releasing factor microinjected into the medial anterior cortex revealed a seizure-related "boxing" behavior elicited by carbachol, which was potentiated by coinjection with substance P and antagonized by coinjection with corticotropin releasing factor. Carbachol 188-197 corticotropin releasing hormone Rattus norvegicus 289-319 3450542-13 1987 CCK and carbachol in their higher concentrations regulated muscarinic receptor and CCK receptor, respectively. Carbachol 8-17 cholecystokinin Homo sapiens 83-86 3435823-3 1987 We demonstrated that neurons in both the CA1 area and dentate gyrus could independently generate carbachol-induced (type 2) electroencephalogram (EEG) theta-activity. Carbachol 97-106 carbonic anhydrase 1 Homo sapiens 41-44 3678079-9 1987 On the other hand, a subgroup of sarcoidosis patients with raised serum IgE levels had a significantly lower stimulation threshold of the respiratory tract to carbachol. Carbachol 159-168 immunoglobulin heavy constant epsilon Homo sapiens 72-75 2446509-2 1987 Acini suspended in pH 7.40 buffer demonstrated cytoplasmic alkalinization of 0.17, 0.14, and 0.15 pH units 2 min after addition of the secretagogues carbachol (10(-5) M), caerulein (10(-10) M), and bromo-A23187 (10(-6) M). Carbachol 149-158 glucose-6-phosphate isomerase 1 Mus musculus 19-21 2446509-2 1987 Acini suspended in pH 7.40 buffer demonstrated cytoplasmic alkalinization of 0.17, 0.14, and 0.15 pH units 2 min after addition of the secretagogues carbachol (10(-5) M), caerulein (10(-10) M), and bromo-A23187 (10(-6) M). Carbachol 149-158 glucose-6-phosphate isomerase 1 Mus musculus 98-100 2446509-4 1987 Pretreatment of acini with atropine blocked the pHi rise induced by carbachol; addition of atropine 2 min after the carbachol did not reverse the alkalinization. Carbachol 68-77 glucose-6-phosphate isomerase 1 Mus musculus 48-51 2446509-8 1987 Net amylase release over 30 min in response to 10(-5) M carbachol was sustained at normal levels in buffers of pH varying between 7.7 and 6.5. Carbachol 56-65 glucose-6-phosphate isomerase 1 Mus musculus 111-113 2446509-9 1987 In contrast, cytoplasmic alkalinization in response to carbachol occurred only in buffers with pH values between 7.40 and 7.10. Carbachol 55-64 glucose-6-phosphate isomerase 1 Mus musculus 95-97 3054870-7 1988 By contrast, perifused zein-injected rat pancreases released several times more PP than the controls in response to carbachol stimulation. Carbachol 116-125 pancreatic polypeptide Rattus norvegicus 80-82 2446113-3 1987 In the presence of carbamylcholine, which itself increases [3H]phencyclidine binding affinity, substance P caused a decrease in the affinity of [3H]phencyclidine. Carbachol 19-34 tachykinin 1 Mus musculus 95-106 3450542-16 1987 These effects of CCK and carbachol on receptors were well compatible to the restriction of carbachol or CCK induced amylase secretion by CCK or carbachol. Carbachol 25-34 cholecystokinin Homo sapiens 104-107 3450542-16 1987 These effects of CCK and carbachol on receptors were well compatible to the restriction of carbachol or CCK induced amylase secretion by CCK or carbachol. Carbachol 25-34 cholecystokinin Homo sapiens 104-107 3450542-16 1987 These effects of CCK and carbachol on receptors were well compatible to the restriction of carbachol or CCK induced amylase secretion by CCK or carbachol. Carbachol 91-100 cholecystokinin Homo sapiens 17-20 3450542-16 1987 These effects of CCK and carbachol on receptors were well compatible to the restriction of carbachol or CCK induced amylase secretion by CCK or carbachol. Carbachol 91-100 cholecystokinin Homo sapiens 17-20 2890115-2 1987 In the presence of the cholinesterase inhibitor physostigmine, acetylcholine significantly (p less than 0.05-p less than 0.01) stimulated inositol phosphate formation in a concentration-related fashion: carbachol, but not oxotremorine, produced similar effects. Carbachol 203-212 butyrylcholinesterase Rattus norvegicus 23-37 2821819-4 1987 Carbachol (0.3 microM) and the phorbol ester PMA (1 microM) potentiate the secretory response to VIP (10 nM), forskolin (3 microM), and dibutyryl adenosine 3",5"-cyclic monophosphate (DBcAMP) (0.5 mM) both in the absence and presence of 2.5 mM extracellular calcium. Carbachol 0-9 vasoactive intestinal peptide Rattus norvegicus 97-100 2890115-4 1987 This agent significantly attenuated the stimulatory effect of carbachol on growth hormone (GH) release. Carbachol 62-71 gonadotropin releasing hormone receptor Rattus norvegicus 75-89 2889453-4 1987 Cholinergic agonists, metacholine and carbamylcholine, slightly increased the NGF content in quiescent cells, but showed no effects on growing cells. Carbachol 38-53 nerve growth factor Mus musculus 78-81 2448658-1 1987 Forskolin, a commonly used adenylate cyclase activator, was found to inhibit reversibly the carbachol-induced ion-translocating capacity of the nicotinic acetylcholine receptor (nAChR) on chick myotubes in a dose- (IC50 = 20 microM) and time-dependent manner. Carbachol 92-101 cholinergic receptor nicotinic beta 3 subunit Gallus gallus 144-176 2448658-1 1987 Forskolin, a commonly used adenylate cyclase activator, was found to inhibit reversibly the carbachol-induced ion-translocating capacity of the nicotinic acetylcholine receptor (nAChR) on chick myotubes in a dose- (IC50 = 20 microM) and time-dependent manner. Carbachol 92-101 cholinergic receptor nicotinic beta 3 subunit Gallus gallus 178-183 2448658-4 1987 In contrast, in the presence of carbachol, forskolin inhibited (IC50 = 10 microM) the binding of 3H-phencyclidine, a putative nAChR ion-channel ligand, to Torpedo microsac nAChR. Carbachol 32-41 cholinergic receptor nicotinic beta 3 subunit Gallus gallus 126-131 2448658-7 1987 In conclusion, forskolin blocks the carbachol-mediated increase in permeability of the nAChR channel by (1) binding to the ion-channel (open state) and (2) generally perturbing the plasma membrane function possibly by interfering with the protein-lipid interface. Carbachol 36-45 cholinergic receptor nicotinic beta 3 subunit Gallus gallus 87-92 3651823-1 1987 Carbachol-induced hippocampal EEG theta-rhythms were recorded simultaneously from the CA1 and dentate areas in rat hippocampal brain slices. Carbachol 0-9 carbonic anhydrase 1 Rattus norvegicus 86-89 2448159-3 1987 Secretion in the presence of submaximal ACTH was potentiated with either 100 microM iso-butylmethylxanthine or 0.3 microM carbachol. Carbachol 122-131 proopiomelanocortin Homo sapiens 40-44 2829144-4 1987 Cch (10 microM) inhibits cellular cAMP accumulation and thyroxine (T4) release induced by vasoactive intestinal peptide (VIP), with or without a phosphodiesterase inhibitor. Carbachol 0-3 vasoactive intestinal peptide Homo sapiens 121-124 3596169-8 1987 These results suggest that gastrin G is cosecreted with gastrin in response to carbachol and bombesin, and the stimulation of gastrin and gastrin-G secretion by bombesin does not involve cholinergic neural pathways and may reflect a direct action on gastrin cells. Carbachol 79-88 gastrin Rattus norvegicus 27-34 3596169-8 1987 These results suggest that gastrin G is cosecreted with gastrin in response to carbachol and bombesin, and the stimulation of gastrin and gastrin-G secretion by bombesin does not involve cholinergic neural pathways and may reflect a direct action on gastrin cells. Carbachol 79-88 gastrin Rattus norvegicus 56-63 3596169-8 1987 These results suggest that gastrin G is cosecreted with gastrin in response to carbachol and bombesin, and the stimulation of gastrin and gastrin-G secretion by bombesin does not involve cholinergic neural pathways and may reflect a direct action on gastrin cells. Carbachol 79-88 gastrin Rattus norvegicus 56-63 3596169-8 1987 These results suggest that gastrin G is cosecreted with gastrin in response to carbachol and bombesin, and the stimulation of gastrin and gastrin-G secretion by bombesin does not involve cholinergic neural pathways and may reflect a direct action on gastrin cells. Carbachol 79-88 gastrin Rattus norvegicus 56-63 3596169-8 1987 These results suggest that gastrin G is cosecreted with gastrin in response to carbachol and bombesin, and the stimulation of gastrin and gastrin-G secretion by bombesin does not involve cholinergic neural pathways and may reflect a direct action on gastrin cells. Carbachol 79-88 gastrin Rattus norvegicus 56-63 2442709-1 1987 Three classes of agonists associated with Ca2+-mobilization--alpha 1-adrenergic (methoxamine), muscarinic (carbachol) and peptidergic (substance P, SP)--significantly stimulated the secretion of mucin from enzymatically-dispersed rat submandibular gland acinar cells. Carbachol 107-116 solute carrier family 13 member 2 Rattus norvegicus 195-200 3037024-6 1987 All three of these monoHETEs were shown also to be inhibitors of the cyclic GMP responses to receptors stimulated by carbachol, histamine, thrombin, neurotensin, and bradykinin. Carbachol 117-126 5'-nucleotidase, cytosolic II Mus musculus 76-79 2442709-7 1987 A brief preincubation of cells with carbachol or SP significantly reduced the subsequent isoproterenol (IPR)-provoked secretion of mucin, whereas methoxamine plus IPR stimulated an additive response. Carbachol 36-45 solute carrier family 13 member 2 Rattus norvegicus 131-136 3496139-1 1987 In human airways synthetic human sequence calcitonin gene-related peptide (hCGRP), a novel peptide produced by alternative processing of mRNA from the calcitonin gene, caused concentration-dependent contraction of human bronchi (EC50 4.9 X 10(-9) M) and was significantly more potent than substance P or carbachol. Carbachol 304-313 calcitonin related polypeptide alpha Homo sapiens 75-80 3037217-3 1987 In oligodendrocytes, the greatest stimulation was produced by carbachol with significant increase also produced by bradykinin, histamine and NE. Carbachol 62-71 kininogen 1 Homo sapiens 115-125 3495533-2 1987 PMA, carbachol, and EGF all stimulated rapid accumulation of mRNA for the proto-oncogenes c-fos and c-myc in the normal cells; in the protein kinase C-deficient cells, carbachol and EGF, but not PMA, retained this effect, which was not mimicked by the calcium ionophore A23187. Carbachol 5-14 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 90-95 3495533-2 1987 PMA, carbachol, and EGF all stimulated rapid accumulation of mRNA for the proto-oncogenes c-fos and c-myc in the normal cells; in the protein kinase C-deficient cells, carbachol and EGF, but not PMA, retained this effect, which was not mimicked by the calcium ionophore A23187. Carbachol 5-14 MYC proto-oncogene, bHLH transcription factor Homo sapiens 100-105 3495533-2 1987 PMA, carbachol, and EGF all stimulated rapid accumulation of mRNA for the proto-oncogenes c-fos and c-myc in the normal cells; in the protein kinase C-deficient cells, carbachol and EGF, but not PMA, retained this effect, which was not mimicked by the calcium ionophore A23187. Carbachol 5-14 epidermal growth factor Homo sapiens 182-185 3495533-2 1987 PMA, carbachol, and EGF all stimulated rapid accumulation of mRNA for the proto-oncogenes c-fos and c-myc in the normal cells; in the protein kinase C-deficient cells, carbachol and EGF, but not PMA, retained this effect, which was not mimicked by the calcium ionophore A23187. Carbachol 168-177 epidermal growth factor Homo sapiens 20-23 3495533-5 1987 Both PMA and carbachol promoted the phosphorylation of the ribosomal protein S6 and activated an S6 protein kinase in the normal but not in the protein kinase C-deficient cells. Carbachol 13-22 ribosomal protein S6 Homo sapiens 59-79 3495533-7 1987 We conclude that, in 1321-N1 cells, induction of c-fos and c-myc mRNA can occur through a protein kinase C-dependent pathway and one or more independent pathways, exemplified by the responses to carbachol and EGF in the protein kinase C-deficient cells. Carbachol 195-204 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 49-54 3495533-7 1987 We conclude that, in 1321-N1 cells, induction of c-fos and c-myc mRNA can occur through a protein kinase C-dependent pathway and one or more independent pathways, exemplified by the responses to carbachol and EGF in the protein kinase C-deficient cells. Carbachol 195-204 MYC proto-oncogene, bHLH transcription factor Homo sapiens 59-64 2441609-3 1987 In vitro secretion of lingual lipase and amylase stimulated by the cholinergic agonist, carbamylcholine chloride (carbachol), was 28.3 +/- 1.7, 48.0 +/- 3.2, and 55.9 +/- 2.4% and 18.1 +/- 1.7, 26.4 +/- 3.0, and 28.0 +/- 2.5%, respectively, for 30-, 60-, and 90-min incubations. Carbachol 88-112 lipase F, gastric type Rattus norvegicus 22-36 2441609-3 1987 In vitro secretion of lingual lipase and amylase stimulated by the cholinergic agonist, carbamylcholine chloride (carbachol), was 28.3 +/- 1.7, 48.0 +/- 3.2, and 55.9 +/- 2.4% and 18.1 +/- 1.7, 26.4 +/- 3.0, and 28.0 +/- 2.5%, respectively, for 30-, 60-, and 90-min incubations. Carbachol 114-123 lipase F, gastric type Rattus norvegicus 22-36 2439077-1 1987 Glucose, forskolin, IBMX and carbachol all stimulated insulin release from freshly obtained human insulinoma cells. Carbachol 29-38 insulin Homo sapiens 54-61 2439077-3 1987 On the other hand, of all the insulin secretagogues examined, only carbachol stimulated the formation of 3H-inositol trisphosphate in these cells. Carbachol 67-76 insulin Homo sapiens 30-37 3033212-1 1987 The effects of phorbol 12-myristate 13-acetate (PMA) on carbamylcholine (CBC)-induced [3H]cyclic GMP formation in mouse neuroblastoma cells (clone N1E-115) were studied. Carbachol 56-71 5'-nucleotidase, cytosolic II Mus musculus 97-100 3033212-1 1987 The effects of phorbol 12-myristate 13-acetate (PMA) on carbamylcholine (CBC)-induced [3H]cyclic GMP formation in mouse neuroblastoma cells (clone N1E-115) were studied. Carbachol 73-76 5'-nucleotidase, cytosolic II Mus musculus 97-100 3477639-10 1987 In Ca2+-free bathing media, carbachol produced a transient contraction accompanied by a transient intracellular Ca2+ spike indicating release of Ca2+ from intracellular storage sites. Carbachol 28-37 carbonic anhydrase 2 Homo sapiens 3-6 3477639-10 1987 In Ca2+-free bathing media, carbachol produced a transient contraction accompanied by a transient intracellular Ca2+ spike indicating release of Ca2+ from intracellular storage sites. Carbachol 28-37 carbonic anhydrase 2 Homo sapiens 112-115 3477639-10 1987 In Ca2+-free bathing media, carbachol produced a transient contraction accompanied by a transient intracellular Ca2+ spike indicating release of Ca2+ from intracellular storage sites. Carbachol 28-37 carbonic anhydrase 2 Homo sapiens 112-115 2981050-10 1987 Carbamylcholine (0.5 mM) was the most potent stimulus used evoking early rises in Ins-1,4,5-P3 and Ins-1,3,4,5-P4 (2 s) followed by Ins-1,3,4-P3 (10 s), effects which were only partially dependent on extracellular Ca2+. Carbachol 0-15 insulin 1 Rattus norvegicus 82-87 3106347-8 1987 The effects of A23187, carbachol, and cholecystokinin on cells preincubated with cholera toxin were abolished by removing extracellular calcium or by adding the calmodulin inhibitor trifluoperazine. Carbachol 23-32 calmodulin 1 Homo sapiens 161-171 2884697-4 1987 Inclusion of carbachol (2.5 X 10(-6) M) in culture medium increased medium gastrin concentration by 106 +/- 28% (P less than 0.01); addition of specific antibodies to gastrin-releasing peptide to the culture medium did not affect carbachol-stimulated gastrin release. Carbachol 13-22 gastrin Rattus norvegicus 75-82 3101510-0 1987 Carbachol regulates cholecystokinin receptor on pancreatic acinar cells. Carbachol 0-9 cholecystokinin Rattus norvegicus 20-35 2955256-2 1987 ANF was found to inhibit the carbachol-stimulated synthesis of catecholamines from their labelled [3H]tyrosine precursor in an organ suspension of the rat superior cervical ganglia in vitro. Carbachol 29-38 natriuretic peptide A Rattus norvegicus 0-3 2885761-3 1987 Imipramine (4 days) resulted in a marked inhibition of the ability of [D-Ala2, D-Leu5] enkephalin to decrease electrically evoked contractions of the vas deferens (presynaptic opioid receptor response) but did not significantly affect the carbachol-induced increase in electrically evoked contractions (muscarinic receptor response). Carbachol 239-248 proenkephalin Rattus norvegicus 87-97 2437768-5 1987 Galanin also impaired the plasma insulin response to either glucose or the cholinergic agonist carbachol. Carbachol 95-104 galanin and GMAP prepropeptide Mus musculus 0-7 2435168-1 1987 In dispersed acini from rat pancreas, it was found that bovine pancreatic polypeptide (BPP) and its C-fragment hexapeptide amide (PP-6), at concentrations of 0.1 and 30 microM, respectively, could significantly inhibit amylase secretion stimulated by carbachol (P less than 0.01 or 0.05, respectively), and this inhibition by BPP was dose dependent. Carbachol 251-260 pancreatic polypeptide Bos taurus 63-85 2981050-10 1987 Carbamylcholine (0.5 mM) was the most potent stimulus used evoking early rises in Ins-1,4,5-P3 and Ins-1,3,4,5-P4 (2 s) followed by Ins-1,3,4-P3 (10 s), effects which were only partially dependent on extracellular Ca2+. Carbachol 0-15 insulin 1 Rattus norvegicus 99-104 2981050-10 1987 Carbamylcholine (0.5 mM) was the most potent stimulus used evoking early rises in Ins-1,4,5-P3 and Ins-1,3,4,5-P4 (2 s) followed by Ins-1,3,4-P3 (10 s), effects which were only partially dependent on extracellular Ca2+. Carbachol 0-15 insulin 1 Rattus norvegicus 99-104 3541715-11 1987 Bradykinin at 10(-4) caused only 21.5 +/- 5.5% of the maximal carbamylcholine contraction in 11 of 18 airways. Carbachol 62-77 kininogen 1 Homo sapiens 0-10 3329090-5 1987 Glucagon secretion stimulated by the cholinergic agonist carbachol was modestly inhibited by NPY at 4.25 nmol/kg (P less than 0.01) but not affected by CGRP. Carbachol 57-66 neuropeptide Y Mus musculus 93-96 3329090-7 1987 Insulin secretion stimulated by carbachol was inhibited by CGRP (P less than 0.01) but not affected by NPY, whereas terbutaline-induced insulin secretion was inhibited by both NPY (P less than 0.05) and CGRP (P less than 0.01). Carbachol 32-41 calcitonin/calcitonin-related polypeptide, alpha Mus musculus 59-63 3040486-2 1987 Stimulation of muscarinic receptor by carbachol (1 mM) resulted in a decrease in 32Pi incorporation into phosphatidylinositol-4,5-bisphophaphate (TPI) and phosphatidylinositol-4-phosphate (DPI), and an increase in 32Pi incorporation into phosphatidylinositol (PI) and phosphatidic acid (PA), whereas no significant effect on other membrane phospholipids was found. Carbachol 38-47 triosephosphate isomerase 1 Rattus norvegicus 146-149 3101510-3 1987 Simultaneous addition of carbamylcholine inhibited binding of CCK to acini due to an apparent loss of high affinity CCK binding sites. Carbachol 25-40 cholecystokinin Rattus norvegicus 116-119 3101510-8 1987 These findings, at least in part, account for the inhibition of the CCK-induced stimulation of amylase secretion by carbamylcholine. Carbachol 116-131 cholecystokinin Rattus norvegicus 68-71 20501187-0 1987 Spontaneous and carbachol-evoked in vivo secretion of acetylcholinesterase from the hippocampus of the rat. Carbachol 16-25 acetylcholinesterase Rattus norvegicus 54-74 20501187-2 1987 Local perfusion with 10(?5)M carbachol evoked an increase of 104% in acetylcholinesterase release with no accompanying change in butyrylcholinesterase or lactic dehydrogenase. Carbachol 29-38 acetylcholinesterase Rattus norvegicus 69-89 20501187-3 1987 Local or systemic atropine sulphate blocked the carbachol-evoked increase in acetylcholinesterase release, whilst gallamine had no effect. Carbachol 48-57 acetylcholinesterase Rattus norvegicus 77-97 20501187-4 1987 Local perfusion of ?-aminobutyric acid (10(?4)M) also blocked the carbachol-evoked release of acetylcholinesterase but had no effect on the spontaneous release. Carbachol 66-75 acetylcholinesterase Rattus norvegicus 94-114 3671348-6 1987 The secretion of lipase and colipase from isolated pancreatic acini was also found to parallel a C/L ratio equal to 0.6 in the basal release and to a ratio between 0.5 and 0.6 after stimulation with CCK, secretin, or carbachol. Carbachol 217-226 lipase G, endothelial type Rattus norvegicus 17-23 3671348-6 1987 The secretion of lipase and colipase from isolated pancreatic acini was also found to parallel a C/L ratio equal to 0.6 in the basal release and to a ratio between 0.5 and 0.6 after stimulation with CCK, secretin, or carbachol. Carbachol 217-226 colipase Rattus norvegicus 28-36 3554168-4 1987 Furthermore, at a low dose level without effect on basal plasma insulin levels, GRP was found to potentiate the insulin response to both glucose (by 40%; p less than 0.05) and to the cholinergic agonist carbachol (by 57%; p less than 0.01). Carbachol 203-212 gastrin releasing peptide Mus musculus 80-83 3554168-5 1987 Also, GRP was at this dose level found to potentiate the glucagon response to carbachol (p less than 0.01). Carbachol 78-87 gastrin releasing peptide Mus musculus 6-9 3554168-7 1987 Moreover, methylatropine given at a dose level that totally abolishes carbachol-induced insulin secretion inhibited GRP-induced insulin secretion by 39% (p less than 0.05) and GRP-induced glucagon secretion by 25% (p less than 0.01). Carbachol 70-79 gastrin releasing peptide Mus musculus 116-119 3101510-1 1987 We have examined the effect of carbamylcholine on the binding of cholecystokinin (CCK) to dispersed acini from rat pancreas. Carbachol 31-46 cholecystokinin Rattus norvegicus 65-80 3101510-1 1987 We have examined the effect of carbamylcholine on the binding of cholecystokinin (CCK) to dispersed acini from rat pancreas. Carbachol 31-46 cholecystokinin Rattus norvegicus 82-85 3101510-3 1987 Simultaneous addition of carbamylcholine inhibited binding of CCK to acini due to an apparent loss of high affinity CCK binding sites. Carbachol 25-40 cholecystokinin Rattus norvegicus 62-65 3569746-2 1986 Gastrin secretion was significantly stimulated by exogenous bombesin at a dose of 10(-8) M. Atropine 10(-6) M, which abolished the action of the cholinergic agent carbachol to stimulate gastrin secretion, had no effect on bombesin-stimulated gastrin secretion. Carbachol 163-172 gastrin Rattus norvegicus 0-7 3432293-4 1987 In contrast, a reduction of AChR levels occurs due to prolonged treatment with caffeine or carbamylcholine. Carbachol 91-106 cholinergic receptor nicotinic delta subunit Gallus gallus 28-32 3099303-8 1986 Carbocholine injection led to a significant decrease in brain temperature in response to TRH administration. Carbachol 0-12 thyrotropin releasing hormone Rattus norvegicus 89-92 3310159-4 1987 On the control day, the dose of carbachol required to reduce the partial expiratory flow volume at 25% of vital capacity (V25p) by at least 25% was established. Carbachol 32-41 immunoglobulin lambda variable 3-7 (pseudogene) Homo sapiens 122-126 2435394-3 1986 When cultures were incubated for 3 days with substance P and carbachol, a cholinergic agonist, substance P (10(-6) M, and greater) completely inhibited the increase in tyrosine hydroxylase activity normally induced by carbachol. Carbachol 61-70 tachykinin precursor 1 Bos taurus 95-106 2435394-3 1986 When cultures were incubated for 3 days with substance P and carbachol, a cholinergic agonist, substance P (10(-6) M, and greater) completely inhibited the increase in tyrosine hydroxylase activity normally induced by carbachol. Carbachol 218-227 tachykinin precursor 1 Bos taurus 45-56 2435394-3 1986 When cultures were incubated for 3 days with substance P and carbachol, a cholinergic agonist, substance P (10(-6) M, and greater) completely inhibited the increase in tyrosine hydroxylase activity normally induced by carbachol. Carbachol 218-227 tachykinin precursor 1 Bos taurus 95-106 2435394-4 1986 Long-term stimulation with carbachol also depleted endogenous catecholamines from the cells and substance P prevented this carbachol-induced depletion of catecholamine content. Carbachol 123-132 tachykinin precursor 1 Bos taurus 96-107 3569746-2 1986 Gastrin secretion was significantly stimulated by exogenous bombesin at a dose of 10(-8) M. Atropine 10(-6) M, which abolished the action of the cholinergic agent carbachol to stimulate gastrin secretion, had no effect on bombesin-stimulated gastrin secretion. Carbachol 163-172 gastrin Rattus norvegicus 186-193 3569746-2 1986 Gastrin secretion was significantly stimulated by exogenous bombesin at a dose of 10(-8) M. Atropine 10(-6) M, which abolished the action of the cholinergic agent carbachol to stimulate gastrin secretion, had no effect on bombesin-stimulated gastrin secretion. Carbachol 163-172 gastrin Rattus norvegicus 242-249 3022747-4 1986 In rat striatal slices carbachol significantly inhibited the VIP-stimulated increase in cyclic AMP. Carbachol 23-32 vasoactive intestinal peptide Rattus norvegicus 61-64 3791067-2 1986 Choline acetyltransferase (ChAT) activity was measured radiometrically using [1-14C]acetyl-coenzyme A as the substrate, muscarinic binding was assayed with [3H]quinuclidinyl benzilate, and the proportion of binding associated with high affinity agonist sites was measured by carbamylcholine displacement of the radioligand. Carbachol 275-290 choline O-acetyltransferase Homo sapiens 27-31 3782086-6 1986 In contrast, both variants of desmin, synemin, caldesmon, and 5 cytosolic proteins are phosphorylated at varying rates and remain phosphorylated for the duration of carbachol action. Carbachol 165-174 desmin Bos taurus 30-36 3782086-6 1986 In contrast, both variants of desmin, synemin, caldesmon, and 5 cytosolic proteins are phosphorylated at varying rates and remain phosphorylated for the duration of carbachol action. Carbachol 165-174 synemin Bos taurus 38-45 2877911-1 1986 Gastrin release was significantly stimulated by the cholinergic agent carbachol at doses of 10(-4) M, 10(-5) M, and 10(-6) M. Peak stimulation was observed at 10(-5) M. Gastrin release was also significantly stimulated by bombesin at a dose of 10(-8) M, and 10(-6) M atropine which abolished the effect of carbachol in stimulating gastrin release had no effect on the bombesin-stimulated gastrin release. Carbachol 70-79 gastrin Rattus norvegicus 0-7 3094467-8 1986 Since carbachol, CCK-8, and A23187, which are believed to act via calcium-calmodulin, also stimulated pepsinogen secretion, this system, too, presumably plays a substantial role. Carbachol 6-15 pepsin II-2/3 Oryctolagus cuniculus 102-112 2428936-2 1986 In the presence of 1 mM 3-isobutyl-1-methylxanthine, dopamine D-2, muscarinic cholinergic, and opiate receptor stimulation by RU 24926, carbachol, and morphine (all at 10(-8)-10(-5) M), respectively, inhibited the increase in cyclic AMP accumulation in slices of rat striatum due to dopamine D-1 receptor stimulation by 1 microM SKF 38393. Carbachol 136-145 solute carrier family 3 member 1 Rattus norvegicus 62-110 2879748-6 1986 Ba2+ (2 mM) also stimulated the releases of VIP-LI and CA from the adrenal gland Carbachol (10(-4)M) stimulated CA secretion as much as high potassium and Ba2+, but the magnitude of VIP-LI release was lower. Carbachol 81-90 vasoactive intestinal peptide Bos taurus 44-47 2879748-6 1986 Ba2+ (2 mM) also stimulated the releases of VIP-LI and CA from the adrenal gland Carbachol (10(-4)M) stimulated CA secretion as much as high potassium and Ba2+, but the magnitude of VIP-LI release was lower. Carbachol 81-90 vasoactive intestinal peptide Bos taurus 182-185 2435897-5 1986 Methionine enkephalin ([Met]-enkephalin) blocked the actions of all the above peptides as well as the effects of DL-octopamine and carbachol. Carbachol 131-140 proopiomelanocortin Homo sapiens 24-39 2435394-7 1986 These results indicate that substance P"s effects are relatively specific for the carbachol-induced increased in tyrosine hydroxylase activity and that the primary site of action of substance P is not a site common to the mechanism of tyrosine hydroxylase induction by carbachol and 8-bromoadenosine 3":5"-cyclic monophosphate. Carbachol 82-91 tachykinin precursor 1 Bos taurus 28-39 2435394-7 1986 These results indicate that substance P"s effects are relatively specific for the carbachol-induced increased in tyrosine hydroxylase activity and that the primary site of action of substance P is not a site common to the mechanism of tyrosine hydroxylase induction by carbachol and 8-bromoadenosine 3":5"-cyclic monophosphate. Carbachol 269-278 tachykinin precursor 1 Bos taurus 28-39 3779216-4 1986 In guinea-pig taenia, harmaline inhibited the 45.4 mM K+-induced contraction with an IC50 of 6.8 X 10(-5) M and the carbachol (10(-6) M)-induced contraction with an IC50 of 7.0 X 10(-5) M. The inhibitory effects on both high K+- and carbachol-induced contractions were antagonized by raising the external Ca2+ concentrations but not by Bay K 8644. Carbachol 116-125 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 305-308 2877911-1 1986 Gastrin release was significantly stimulated by the cholinergic agent carbachol at doses of 10(-4) M, 10(-5) M, and 10(-6) M. Peak stimulation was observed at 10(-5) M. Gastrin release was also significantly stimulated by bombesin at a dose of 10(-8) M, and 10(-6) M atropine which abolished the effect of carbachol in stimulating gastrin release had no effect on the bombesin-stimulated gastrin release. Carbachol 70-79 gastrin Rattus norvegicus 169-176 3535012-6 1986 Carbachol (250 micrograms subcutaneously) increased PP release by 62% but did not affect the other hormones. Carbachol 0-9 familial progressive hyperpigmentation 1 Homo sapiens 52-54 2877911-1 1986 Gastrin release was significantly stimulated by the cholinergic agent carbachol at doses of 10(-4) M, 10(-5) M, and 10(-6) M. Peak stimulation was observed at 10(-5) M. Gastrin release was also significantly stimulated by bombesin at a dose of 10(-8) M, and 10(-6) M atropine which abolished the effect of carbachol in stimulating gastrin release had no effect on the bombesin-stimulated gastrin release. Carbachol 70-79 gastrin Rattus norvegicus 331-338 2877911-1 1986 Gastrin release was significantly stimulated by the cholinergic agent carbachol at doses of 10(-4) M, 10(-5) M, and 10(-6) M. Peak stimulation was observed at 10(-5) M. Gastrin release was also significantly stimulated by bombesin at a dose of 10(-8) M, and 10(-6) M atropine which abolished the effect of carbachol in stimulating gastrin release had no effect on the bombesin-stimulated gastrin release. Carbachol 70-79 gastrin Rattus norvegicus 388-395 2877911-1 1986 Gastrin release was significantly stimulated by the cholinergic agent carbachol at doses of 10(-4) M, 10(-5) M, and 10(-6) M. Peak stimulation was observed at 10(-5) M. Gastrin release was also significantly stimulated by bombesin at a dose of 10(-8) M, and 10(-6) M atropine which abolished the effect of carbachol in stimulating gastrin release had no effect on the bombesin-stimulated gastrin release. Carbachol 306-315 gastrin Rattus norvegicus 0-7 2877911-1 1986 Gastrin release was significantly stimulated by the cholinergic agent carbachol at doses of 10(-4) M, 10(-5) M, and 10(-6) M. Peak stimulation was observed at 10(-5) M. Gastrin release was also significantly stimulated by bombesin at a dose of 10(-8) M, and 10(-6) M atropine which abolished the effect of carbachol in stimulating gastrin release had no effect on the bombesin-stimulated gastrin release. Carbachol 306-315 gastrin Rattus norvegicus 169-176 3741886-3 1986 Carbachol, histamine, gastrin and CCK-8 increased parietal cell [Ca2+]i with the response to carbachol greater than CCK -8 = histamine = gastrin. Carbachol 0-9 gastrin Homo sapiens 137-144 3755789-7 1986 NEM at 30 microM enhanced the carbamylcholine stimulated labeling of phosphatidic acid from 32Pi in nerve endings from rat forebrain, suggesting that the low affinity M2-AChr may mediate at least a part of the inositide response to cholinergic stimulation. Carbachol 30-45 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 170-174 3015710-7 1986 Carbachol inhibited L-tryptophan-stimulated cholecystokinin release in a dose-dependent manner. Carbachol 0-9 cholecystokinin Canis lupus familiaris 44-59 3015710-11 1986 Stimulated cholecystokinin release is inhibited by the muscarinic agonist carbachol. Carbachol 74-83 cholecystokinin Canis lupus familiaris 11-26 3093455-2 1986 Cholinergic airway responsiveness was determined by measuring the carbachol dose required to increase specific lung resistance (sRL) 150% (i.e., PC150). Carbachol 66-75 sarcalumenin Ovis aries 128-131 3741886-3 1986 Carbachol, histamine, gastrin and CCK-8 increased parietal cell [Ca2+]i with the response to carbachol greater than CCK -8 = histamine = gastrin. Carbachol 93-102 gastrin Homo sapiens 22-29 3741886-11 1986 13, G814-G823) we conclude that: carbachol, CCK-8, gastrin and histamine increase parietal cell [Ca2+]i initially by release of Ca2+ from the same intracellular store(s); the release of [Ca2+]i in response to carbachol and CCK-8 in both chief and parietal cells appear to be mediated by IP3; however, other mechanisms may be involved in histamine-induced release of parietal cell Ca2+. Carbachol 209-218 gastrin Homo sapiens 51-58 2426956-7 1986 Raising intracellular Ca2+ by the calcium ionophore A23187 evoked the same pattern of Cl- loss and O2 uptake as carbachol. Carbachol 112-121 carbonic anhydrase 2 Rattus norvegicus 22-25 2426956-9 1986 Carbachol raises intracellular Ca2+, causing an increased Cl- permeability of the luminal membrane, resulting in a net Cl- efflux. Carbachol 0-9 carbonic anhydrase 2 Rattus norvegicus 31-34 2874742-1 1986 Intracerebroventricular (icv) administration of carbachol into conscious rats evoked a substantial increase in vasopressin secretion and blood pressure in a dose-dependent manner. Carbachol 48-57 arginine vasopressin Rattus norvegicus 111-122 3525125-9 1986 At this dose level, CGRP inhibited the insulin secretory response to carbachol, leaving that to glucose unaffected. Carbachol 69-78 calcitonin/calcitonin-related polypeptide, alpha Mus musculus 20-24 3017442-4 1986 Adrenocortical spermidine N1-acetyltransferase was also induced by carbamylcholine, 2-deoxyglucose, apomorphine and piribedil, drugs that are known to cause induction of ornithine decarboxylase in that organ. Carbachol 67-82 ornithine decarboxylase 1 Rattus norvegicus 170-193 2873868-2 1986 The present work shows that 2-amino-7-phosphonoheptanoic acid (2-APH), a specific antagonist of receptors activated by n-methyl-D-aspartic acid (NMDA), when microinjected into DPC reduces the incidence of clonic seizures elicited by bicuculline, carbachol or kainic acid microinjected into the same site. Carbachol 246-255 acylaminoacyl-peptide hydrolase Homo sapiens 65-68 3530597-0 1986 Stimulation by glucose and carbamylcholine of phospholipase A2 in pancreatic islets. Carbachol 27-42 phospholipase A2 group IB Homo sapiens 46-62 2431178-0 1986 [Effect of CDP-choline on the action of cholecystokinin and carbachol to stimulate amylase secretion from dispersed rat pancreatic acini]. Carbachol 60-69 cut-like homeobox 1 Rattus norvegicus 11-14 3018477-5 1986 Carbachol mediated cyclic GMP formation with an equilibrium dissociation constant (KD) of 325 microM and cyclic AMP reductions with a KD value of 13 microM. Carbachol 0-9 5'-nucleotidase, cytosolic II Mus musculus 26-29 3014395-1 1986 Stimulation of cholinergic fibers or bath application of carbachol (0.1-10 microM) induced a slow excitability increase in CA3 neurons and dentate granule cells of hippocampal slices. Carbachol 57-66 carbonic anhydrase 3 Cavia porcellus 123-126 2871836-4 1986 Isoproterenol, a beta-adrenergic agonist, and carbachol, a cholinergic agonist produced a 3-fold increase in plasma ANF levels which were constant until the end of the infusion period. Carbachol 46-55 natriuretic peptide A Rattus norvegicus 116-119 3742153-0 1986 The hypothermic action of carbachol in the rat brain periaqueductal grey area may involve neurotensin. Carbachol 26-35 neurotensin Rattus norvegicus 90-101 3768572-3 1986 Airway responsiveness to carbachol was determined prior to and 24 h after antigen challenge by measuring specific lung resistance (sRL) after administering increasing doses of carbachol aerosol (0.5, 1 and 2.5% w/v) and determining the concentration of carbachol needed to increase sRL 150% over baseline (PC150). Carbachol 25-34 sarcalumenin Ovis aries 131-134 3768572-3 1986 Airway responsiveness to carbachol was determined prior to and 24 h after antigen challenge by measuring specific lung resistance (sRL) after administering increasing doses of carbachol aerosol (0.5, 1 and 2.5% w/v) and determining the concentration of carbachol needed to increase sRL 150% over baseline (PC150). Carbachol 25-34 sarcalumenin Ovis aries 282-285 3084483-7 1986 The criteria for the receptor-Ni interaction, i.e. carbachol stimulation of the activities of Ni and the GTP gamma S effect on carbachol binding, were no longer observed, when this IAP-treated Ni, instead of the nontreated Ni, was reconstituted into vesicles, though there was no difference between IAP-treated and nontreated Ni in their basal activities observable without carbachol. Carbachol 51-60 Cd47 molecule Rattus norvegicus 181-184 3084483-7 1986 The criteria for the receptor-Ni interaction, i.e. carbachol stimulation of the activities of Ni and the GTP gamma S effect on carbachol binding, were no longer observed, when this IAP-treated Ni, instead of the nontreated Ni, was reconstituted into vesicles, though there was no difference between IAP-treated and nontreated Ni in their basal activities observable without carbachol. Carbachol 127-136 Cd47 molecule Rattus norvegicus 181-184 3084483-7 1986 The criteria for the receptor-Ni interaction, i.e. carbachol stimulation of the activities of Ni and the GTP gamma S effect on carbachol binding, were no longer observed, when this IAP-treated Ni, instead of the nontreated Ni, was reconstituted into vesicles, though there was no difference between IAP-treated and nontreated Ni in their basal activities observable without carbachol. Carbachol 127-136 Cd47 molecule Rattus norvegicus 181-184 3700408-5 1986 Stimulation by carbamylcholine, nicotine, 56 mM K+, or 2 mM Ba2+ led to the incorporation of 32P into a 37-kDa protein that had previously been characterized as a substrate for protein kinase C in vitro (chromobindin 9, or CB9; Summers, T. A., and Creutz, C. E. (1985) J. Biol. Carbachol 15-30 annexin A1 Homo sapiens 204-218 3009779-3 1986 Adenosine (10(-6) M) inhibited the actions of histamine (10(-14) M) and gastrin (2.5 X 10(-12) M) by 69 and 67%, respectively, but not that of dibutyryl cyclic AMP (10(-16) M) or carbachol (10(-9) M). Carbachol 179-188 gastrin Cavia porcellus 72-79 3724745-0 1986 Two components of carbamylcholine-induced loss of nicotinic acetylcholine receptor function in the neuronal cell line PC12. Carbachol 18-33 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 50-82 2871836-8 1986 This suggests that the rise in plasma ANF induced by isoproterenol and carbachol may be secondary to hemodynamic changes and not to direct receptor stimulation, and may play a role in the observed natriuresis. Carbachol 71-80 natriuretic peptide A Rattus norvegicus 38-41 2869695-3 1986 Inclusion of carbachol (2.5 X 10(-6) M) in the culture medium increased media gastrin concentrations from 3.29 +/- 0.76 (SE) (control) to 6.77 +/- 0.76 ng/mg tissue prot (P less than 0.02). Carbachol 13-22 gastrin Rattus norvegicus 78-85 2869695-4 1986 Rat antral mucosa was then incubated in the presence of GIP (10(-10) to 10(-7) M) to determine its effect on carbachol-stimulated gastrin release. Carbachol 109-118 gastric inhibitory polypeptide Rattus norvegicus 56-59 2869695-4 1986 Rat antral mucosa was then incubated in the presence of GIP (10(-10) to 10(-7) M) to determine its effect on carbachol-stimulated gastrin release. Carbachol 109-118 gastrin Rattus norvegicus 130-137 2869695-5 1986 GIP significantly inhibited carbachol-stimulated gastrin release into the culture media at all concentrations examined. Carbachol 28-37 gastric inhibitory polypeptide Rattus norvegicus 0-3 2869695-5 1986 GIP significantly inhibited carbachol-stimulated gastrin release into the culture media at all concentrations examined. Carbachol 28-37 gastrin Rattus norvegicus 49-56 2869695-6 1986 To determine whether inhibition of carbachol-stimulated gastrin release by GIP was mediated by somatostatin, antral mucosa was incubated in the presence of carbachol, GIP (10(-10) to 10(-7) M), and specific antibodies to somatostatin in excess. Carbachol 35-44 gastrin Rattus norvegicus 56-63 2869695-6 1986 To determine whether inhibition of carbachol-stimulated gastrin release by GIP was mediated by somatostatin, antral mucosa was incubated in the presence of carbachol, GIP (10(-10) to 10(-7) M), and specific antibodies to somatostatin in excess. Carbachol 35-44 gastric inhibitory polypeptide Rattus norvegicus 75-78 2869695-7 1986 Inclusion of antibodies to somatostatin in the culture medium abolished the capacity of GIP (10(9) to 10(-7) M) to inhibit carbachol-stimulated gastrin release. Carbachol 123-132 gastric inhibitory polypeptide Rattus norvegicus 88-91 2869695-7 1986 Inclusion of antibodies to somatostatin in the culture medium abolished the capacity of GIP (10(9) to 10(-7) M) to inhibit carbachol-stimulated gastrin release. Carbachol 123-132 gastrin Rattus norvegicus 144-151 2869695-8 1986 Results of these studies indicate 1) that GIP inhibits carbachol-stimulated gastrin release and 2) that, under the conditions of these experiments, GIP inhibition of gastrin release may be mediated locally through release of antral somatostatin. Carbachol 55-64 gastric inhibitory polypeptide Rattus norvegicus 42-45 2869695-8 1986 Results of these studies indicate 1) that GIP inhibits carbachol-stimulated gastrin release and 2) that, under the conditions of these experiments, GIP inhibition of gastrin release may be mediated locally through release of antral somatostatin. Carbachol 55-64 gastrin Rattus norvegicus 76-83 3958790-0 1986 Loss of acetylcholine receptor clusters induced by treatment of cultured rat myotubes with carbachol. Carbachol 91-100 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 8-30 3958790-1 1986 Prolonged exposure to carbachol disrupts the acetylcholine receptor (AChR) clusters of cultured rat myotubes without causing myotube loss. Carbachol 22-31 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 45-67 3958790-1 1986 Prolonged exposure to carbachol disrupts the acetylcholine receptor (AChR) clusters of cultured rat myotubes without causing myotube loss. Carbachol 22-31 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 69-73 3958790-6 1986 These results are consistent with the idea that cluster loss caused by carbachol and other receptor agonists results from their interaction with the AChR, and the consequent influx of cations into the myotubes. Carbachol 71-80 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 149-153 3958790-7 1986 Several experiments suggest that extracellular Na+ and Ca2+ are required, and that at least Na+ must permeate the AChR ion channel for full cluster loss to occur in the presence of carbachol. Carbachol 181-190 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 114-118 3954930-4 1986 The difference in the time-course of responses to acetylcholine and carbachol may be attributed to differences in the susceptibility of the two drugs to metabolism by acetylcholinesterase; carbachol, unlike acetylcholine, being virtually immune to metabolism by this enzyme. Carbachol 68-77 acetylcholinesterase (Cartwright blood group) Homo sapiens 167-187 3954930-4 1986 The difference in the time-course of responses to acetylcholine and carbachol may be attributed to differences in the susceptibility of the two drugs to metabolism by acetylcholinesterase; carbachol, unlike acetylcholine, being virtually immune to metabolism by this enzyme. Carbachol 189-198 acetylcholinesterase (Cartwright blood group) Homo sapiens 167-187 3003156-5 1986 The carbachol-induced increase in Isc was potentiated by either prostaglandin E1 (PGE1) or vasoactive intestinal polypeptide (VIP), agents that act by increasing cAMP. Carbachol 4-13 vasoactive intestinal peptide Homo sapiens 91-124 3003156-5 1986 The carbachol-induced increase in Isc was potentiated by either prostaglandin E1 (PGE1) or vasoactive intestinal polypeptide (VIP), agents that act by increasing cAMP. Carbachol 4-13 vasoactive intestinal peptide Homo sapiens 126-129 3003156-10 1986 Carbachol"s effect on Cl- secretion is greatly augmented in the presence of VIP or PGE1, which open a cAMP-sensitive Cl- channel on the apical membrane, accounting for a potentiated response. Carbachol 0-9 vasoactive intestinal peptide Homo sapiens 76-79 2868120-2 1986 Using intact cells, we found that although ethanol had no effect on basal levels of cyclic GMP synthesis, it rapidly inhibited in a concentration-dependent manner cyclic GMP synthesis mediated by the agonists histamine (histamine H1 receptor) and carbachol (low-affinity muscarinic receptor) and by ionophore X537A and melittin, agents which bypass these receptors. Carbachol 247-256 5'-nucleotidase, cytosolic II Mus musculus 170-173 2867688-7 1986 Resting VIP-LI release was enhanced by cholinergic agents (carbachol). Carbachol 59-68 vasoactive intestinal peptide Homo sapiens 8-11 3107454-5 1986 In contrast, carbachol, which does not depolarize, elicits Ptd Ins 4,5-P2 hydrolysis, a reaction catalyzed by phospholipase C. The generation of Ins 1,4,5-P3 in this instance is Ca2+ independent, but appears to involve a GTP-binding protein. Carbachol 13-22 hydroxycarboxylic acid receptor 3 Homo sapiens 221-240 2869808-8 1986 Methylene blue, a drug reported to inhibit guanylate cyclase, in a concentration of 10 microM selectively abolished the relaxation produced by carbachol. Carbachol 143-152 guanylate cyclase Bos taurus 43-60 3942871-2 1986 Infusions of eserine or carbachol elicited hippocampal theta activity when made in areas containing high levels of cholinergic markers: the stratum oriens and radiatum of the CA1 and CA3, the stratum moleculare and stratum granulosum of the dentate gyrus and the infragranular region of the hilus. Carbachol 24-33 carbonic anhydrase 1 Rattus norvegicus 175-178 3942871-2 1986 Infusions of eserine or carbachol elicited hippocampal theta activity when made in areas containing high levels of cholinergic markers: the stratum oriens and radiatum of the CA1 and CA3, the stratum moleculare and stratum granulosum of the dentate gyrus and the infragranular region of the hilus. Carbachol 24-33 carbonic anhydrase 3 Rattus norvegicus 183-186 3942871-5 1986 Infusions of eserine or carbachol in the vicinity of the hippocampal fissure, the stratum lacunosum/moleculare of the CA1 or CA3, in the deep regions of the hilus, and in the lateral ventricle and overlying neocortex, were also without effect. Carbachol 24-33 carbonic anhydrase 1 Rattus norvegicus 118-121 3002355-6 1985 Carbachol, which inhibits ADH-induced water flow, also stimulated 32P incorporation into PA and PI. Carbachol 0-9 arginine vasopressin Homo sapiens 26-29 2876015-1 1986 Rat antral mucosae maintained for 6 h in organ culture responded to carbamylcholine with a significant increase in endogenous cyclic GMP production and gastrin secretion. Carbachol 68-83 gastrin Rattus norvegicus 152-159 2876015-2 1986 The acetylcholine analogue exerted a stimulatory action within a defined concentration range: exposure of antral explants to carbachol concentrations greater than the optimal stimulatory dose was accompanied by a marked decrease in both cyclic GMP production and gastrin release. Carbachol 125-134 gastrin Rattus norvegicus 263-270 2418867-8 1985 Treatment of cultures with 5 ng/mL IAP for 24 h completely blocked the stimulation of 86Rb+ efflux evoked by carbachol. Carbachol 109-118 baculoviral IAP repeat-containing 7 (livin) Mus musculus 32-38 2414402-0 1985 Effects of substance P on carbachol-stimulated 45Ca2+ uptake into cultured adrenal chromaffin cells. Carbachol 26-35 tachykinin precursor 1 Bos taurus 11-22 2414402-3 1985 Two effects of substance P were observed: (1) Substance P inhibited carbachol-induced 45Ca2+ uptake and 45Ca2+ efflux and (2) substance P protected against desensitization of carbachol-induced 45Ca2+ uptake and 45Ca2+ efflux. Carbachol 68-77 tachykinin precursor 1 Bos taurus 15-26 2414402-3 1985 Two effects of substance P were observed: (1) Substance P inhibited carbachol-induced 45Ca2+ uptake and 45Ca2+ efflux and (2) substance P protected against desensitization of carbachol-induced 45Ca2+ uptake and 45Ca2+ efflux. Carbachol 68-77 tachykinin precursor 1 Bos taurus 46-57 2414402-3 1985 Two effects of substance P were observed: (1) Substance P inhibited carbachol-induced 45Ca2+ uptake and 45Ca2+ efflux and (2) substance P protected against desensitization of carbachol-induced 45Ca2+ uptake and 45Ca2+ efflux. Carbachol 175-184 tachykinin precursor 1 Bos taurus 15-26 2414402-3 1985 Two effects of substance P were observed: (1) Substance P inhibited carbachol-induced 45Ca2+ uptake and 45Ca2+ efflux and (2) substance P protected against desensitization of carbachol-induced 45Ca2+ uptake and 45Ca2+ efflux. Carbachol 175-184 tachykinin precursor 1 Bos taurus 126-137 2414402-5 1985 The results also indicate that substance P"s inhibition of net carbachol-induced 45Ca2+ uptake is due to inhibition of 45Ca2+ uptake rather than enhancement of 45Ca2+ efflux. Carbachol 63-72 tachykinin precursor 1 Bos taurus 31-42 2414402-6 1985 Substance P almost completely inhibited carbachol-induced 45Ca2+ uptake in both Na+-containing and Na+-free media, suggesting that substance P can inhibit the uptake of 45Ca2+ induced by carbachol regardless of whether 45Ca2+ is taken up through voltage-sensitive or acetylcholine receptor-linked channels. Carbachol 40-49 tachykinin precursor 1 Bos taurus 0-11 2414402-6 1985 Substance P almost completely inhibited carbachol-induced 45Ca2+ uptake in both Na+-containing and Na+-free media, suggesting that substance P can inhibit the uptake of 45Ca2+ induced by carbachol regardless of whether 45Ca2+ is taken up through voltage-sensitive or acetylcholine receptor-linked channels. Carbachol 40-49 tachykinin precursor 1 Bos taurus 131-142 2414402-6 1985 Substance P almost completely inhibited carbachol-induced 45Ca2+ uptake in both Na+-containing and Na+-free media, suggesting that substance P can inhibit the uptake of 45Ca2+ induced by carbachol regardless of whether 45Ca2+ is taken up through voltage-sensitive or acetylcholine receptor-linked channels. Carbachol 187-196 tachykinin precursor 1 Bos taurus 0-11 2414402-6 1985 Substance P almost completely inhibited carbachol-induced 45Ca2+ uptake in both Na+-containing and Na+-free media, suggesting that substance P can inhibit the uptake of 45Ca2+ induced by carbachol regardless of whether 45Ca2+ is taken up through voltage-sensitive or acetylcholine receptor-linked channels. Carbachol 187-196 tachykinin precursor 1 Bos taurus 131-142 2414402-8 1985 In addition, substance P"s inhibition of carbachol-induced 45Ca2+ uptake was noncompetitive with respect to Ca2+, were unable to overcome substance P"s inhibition of [3H]-norepinephrine ( [3H]NE) release. Carbachol 41-50 tachykinin precursor 1 Bos taurus 13-24 3879308-4 1985 Quinacrine, a phospholipase A2 inhibitor, methylene blue, a guanylate cyclase inhibitor and tetraethylammonium, a potassium permeability inhibitor, inhibited carbachol-induced relaxation and augmented the magnitude of norepinephrine-induced contraction only when endothelium was present. Carbachol 158-167 phospholipase A2 group IB Rattus norvegicus 14-30 2415168-8 1985 Thus, the VIP-ergic secretory response in the rat parotid gland is associated with a raised intracellular cyclic AMP level and the mobilisation of a different intracellular Ca2+ pool than that mobilised by carbachol. Carbachol 206-215 vasoactive intestinal peptide Rattus norvegicus 10-13 3929859-4 1985 Bradykinin is 1,000-fold more potent than the other agonists tested, which include histamine, norepinephrine, epinephrine, eledoisin-related peptide, arginine-vasopressin, lysine-vasopressin, desmopressin acetate, carbachol, and acetylcholine. Carbachol 214-223 kininogen 1 Homo sapiens 0-10 3933882-0 1985 Stimulation by glucose and carbamylcholine of phospholipase A2 in pancreatic islets. Carbachol 27-42 phospholipase A2 group IB Rattus norvegicus 46-62 3933882-5 1985 These results suggest that carbamylcholine and, to a lesser extent, glucose cause a Ca2+-dependent activation of phospholipase A2 in intact islet cells. Carbachol 27-42 phospholipase A2 group IB Rattus norvegicus 113-129 2992293-3 1985 The inhibitory action of carbachol in the absence of isoproterenol and its attenuation by IAP were not associated with any changes in tissue adenosine 3",5"-cyclic monophosphate (cAMP) levels. Carbachol 25-34 Cd47 molecule Rattus norvegicus 90-93 3930219-0 1985 Thyrotropin-releasing hormone release from rat pancreas is stimulated by serotonin but inhibited by carbachol. Carbachol 100-109 thyrotropin releasing hormone Rattus norvegicus 0-29 3930219-6 1985 Carbachol resulted in a dose-dependent inhibition of TRH release (57% inhibition of release at 10(-8) M; P less than 0.01 compared to control). Carbachol 0-9 thyrotropin releasing hormone Rattus norvegicus 53-56 2989329-2 1985 C5a also attenuated carbamyl choline-induced drinking, and carbamyl choline inhibited C5a-induced eating, a mutual inhibition characteristic of the adrenergic-cholinergic interactions at this site. Carbachol 20-36 complement C5 Rattus norvegicus 0-3 2989329-2 1985 C5a also attenuated carbamyl choline-induced drinking, and carbamyl choline inhibited C5a-induced eating, a mutual inhibition characteristic of the adrenergic-cholinergic interactions at this site. Carbachol 59-75 complement C5 Rattus norvegicus 86-89 4070020-6 1985 Moreover, a high dose of carbachol could inhibit VIP-induced radioiodine secretion. Carbachol 25-34 vasoactive intestinal polypeptide Mus musculus 49-52 4070020-7 1985 Methylatropine did not influence TSH- or VIP-stimulated radioiodine secretion, but counteracted the inhibitory action of carbachol on TSH- and VIP-induced radioiodine release. Carbachol 121-130 vasoactive intestinal polypeptide Mus musculus 143-146 2994873-8 1985 Pertussis toxin pretreatment attenuated the inhibitory effects of the muscarinic agents on forskolin-stimulated cyclic AMP synthesis and ACTH secretion as well as the inhibitory effect of carbachol on basal ACTH release. Carbachol 188-197 pro-opiomelanocortin-alpha Mus musculus 207-211 4020581-6 1985 From that day on, HDase became sensitive to both pentagastrin and carbachol. Carbachol 66-75 histidine decarboxylase Rattus norvegicus 18-23 2984215-1 1985 In PC12 cells, cultured in the presence of nerve growth factor to increase their complement of muscarinic receptors, treatment with carbachol induces muscarinic receptor-dependent rises in free cytosolic Ca2+ as well as hydrolysis of membrane phosphoinositides. Carbachol 132-141 carbonic anhydrase 2 Rattus norvegicus 204-207 2984215-5 1985 When these cells were then treated with carbachol, their cytosolic concentration of Ca2+ remained at the resting level, whereas inositol-1,4,5-trisphosphate generation was still markedly stimulated. Carbachol 40-49 carbonic anhydrase 2 Rattus norvegicus 84-87 3883800-0 1985 Carbachol modulates GIP-mediated insulin release from rat pancreatic lobules in vitro. Carbachol 0-9 gastric inhibitory polypeptide Rattus norvegicus 20-23 3883800-19 1985 At amino acid concentrations of 21 and 211 mM, but not 2.1 mM, GIP augmented insulin release but again only with carbachol present. Carbachol 113-122 gastric inhibitory polypeptide Rattus norvegicus 63-66 3724745-1 1986 Loss of responsiveness of the neuronal-type nicotinic acetylcholine receptor (nAChR) on PC12 cells, a cell line derived from a rat pheochromocytoma, was induced by exposure to carbamylcholine (carbachol). Carbachol 176-191 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 44-76 3724745-1 1986 Loss of responsiveness of the neuronal-type nicotinic acetylcholine receptor (nAChR) on PC12 cells, a cell line derived from a rat pheochromocytoma, was induced by exposure to carbamylcholine (carbachol). Carbachol 176-191 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 78-83 3724745-1 1986 Loss of responsiveness of the neuronal-type nicotinic acetylcholine receptor (nAChR) on PC12 cells, a cell line derived from a rat pheochromocytoma, was induced by exposure to carbamylcholine (carbachol). Carbachol 193-202 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 44-76 3724745-1 1986 Loss of responsiveness of the neuronal-type nicotinic acetylcholine receptor (nAChR) on PC12 cells, a cell line derived from a rat pheochromocytoma, was induced by exposure to carbamylcholine (carbachol). Carbachol 193-202 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 78-83 2582418-2 1985 BTX enhanced the affinity for the binding of the agonists carbamoylcholine and acetylcholine to the muscarinic receptors in brainstem and ventricle, but not in the cerebral cortex. Carbachol 58-74 cholinergic receptor nicotinic alpha 7 subunit Rattus norvegicus 0-3 2582418-4 1985 Guanyl nucleotides, known to induce interconversion of high-affinity agonist binding sites to the low-affinity state, canceled the effect of BTX on carbamoylcholine and acetylcholine binding. Carbachol 148-164 cholinergic receptor nicotinic alpha 7 subunit Rattus norvegicus 141-144 4017638-2 1985 Carbamyl-choline and the peptides eledoisin and vasoactive intestinal peptide (VIP) stimulated volume flux as well as secretion of the marker proteins indicating duct cell activation. Carbachol 0-16 VIP peptides Oryctolagus cuniculus 79-82 2981284-2 1985 Elevated potassium (56 mM) and carbamylcholine (carbachol, 10(-4) M) cause rapid increases in cyclic AMP levels in the cultures that show a time course similar to that of evoked dopamine release. Carbachol 31-46 transmembrane serine protease 5 Rattus norvegicus 101-104 2981284-2 1985 Elevated potassium (56 mM) and carbamylcholine (carbachol, 10(-4) M) cause rapid increases in cyclic AMP levels in the cultures that show a time course similar to that of evoked dopamine release. Carbachol 48-57 transmembrane serine protease 5 Rattus norvegicus 101-104 3999047-1 1985 In adrenalectomized, deoxycorticosterone-treated and normal rats, injection of angiotensin II through a cannula implanted in the preoptic region caused increased intakes of hypertonic NaCl and water when both fluids were available, whereas injection of carbachol through the same cannula only caused increased water intake. Carbachol 253-262 angiotensinogen Rattus norvegicus 79-93 2982089-4 1985 Pirenzepine inhibited the [3H]cyclic GMP response to carbachol with a KD value of approximately 6 nM, whereas it inhibited the ability of carbachol to reduce prostaglandin E1-mediated elevations in [3H]cyclic AMP levels with a KD value of 93 nM, thus differentiating between two classes of receptors involved in these responses. Carbachol 53-62 5'-nucleotidase, cytosolic II Mus musculus 37-40 2982089-8 1985 These four agonists and bethanecol, which could increase [3H]cyclic GMP levels only 18% as well as acetylcholine, behaved as competitive antagonists in this response to carbachol. Carbachol 169-178 5'-nucleotidase, cytosolic II Mus musculus 68-71 2982089-10 1985 The equilibrium dissociation constants for the partial agonists determined by their inhibition of carbachol in the [3H] cyclic GMP response also agreed well with their respective EC50 values for mediating the [3H]cyclic AMP response. Carbachol 98-107 5'-nucleotidase, cytosolic II Mus musculus 127-130 2982089-11 1985 Thus, the partial agonists bound to the same receptors at which carbachol mediated [3H]cyclic GMP formation, but with KD values about the same as their respective EC50 values for inhibition of prostaglandin E1-mediated [3H]cyclic AMP increases. Carbachol 64-73 5'-nucleotidase, cytosolic II Mus musculus 94-97 2997533-6 1985 Caffeine and carbachol contracted isolated smooth muscle cells and increased intracellular cyclic GMP level. Carbachol 13-22 5'-nucleotidase, cytosolic II Homo sapiens 98-101 2578456-8 1985 In the presence of carbamylcholine or cholecystokinin stimulation, the times required for 40% discharge of labeled chymotrypsinogen 2, trypsinogen, amylase, and procarboxypeptidase B were 98, 102, 148, and 180 min, respectively. Carbachol 19-34 trypsinogen Cavia porcellus 120-131 2416452-10 1985 This pattern of structural and functional adaptation of acinar cells following secretin infusion corresponds to previously described changes following caerulein and carbamylcholine stimulation. Carbachol 165-180 secretin Rattus norvegicus 79-87 2992293-5 1985 The inhibitory action of carbachol on the isoproterenol-induced functional and cAMP responses was also reduced by the IAP treatment. Carbachol 25-34 Cd47 molecule Rattus norvegicus 118-121 2992293-6 1985 The increase in tissue guanosine 3",5"-cyclic monophosphate (cGMP) levels produced by carbachol was likewise abolished by the IAP treatment. Carbachol 86-95 Cd47 molecule Rattus norvegicus 126-129 2992293-7 1985 These results indicate that IAP treatment attenuates the inhibitory actions of carbachol elicited via muscarinic receptors through both cAMP-dependent and cAMP-independent subcellular processes. Carbachol 79-88 Cd47 molecule Rattus norvegicus 28-31 2994150-3 1985 We have reported that lithium ion (Li+) inhibits cyclic GMP formation mediated by the muscarinic receptor agonist, carbachol, in a concentration-dependent manner and that neither ammonium nor sodium ions have such an effect. Carbachol 115-124 5'-nucleotidase, cytosolic II Mus musculus 56-59 2857528-3 1985 Carbachol at a dose of 10(-6) M inhibited SLI and stimulated gastrin secretion. Carbachol 0-9 gastrin Rattus norvegicus 61-68 2857528-5 1985 Pirenzepine caused a progressive parallel rightward shift in the dose-response curves for SLI inhibition and gastrin stimulation by carbachol, suggesting competitive inhibition. Carbachol 132-141 gastrin Rattus norvegicus 109-116 6091776-4 1984 Carbachol produced a steady-state increase of [Ca2+]in and its effect was blocked by atropine and Ca2+ -channel blocking agents. Carbachol 0-9 carbonic anhydrase 2 Homo sapiens 47-50 4047976-5 1985 The acetylcholine agonist carbachol, injected directly into the NTS region, effectively mimicked the actions of intraperitoneally administered CCK on feeding and exploration. Carbachol 26-35 cholecystokinin Rattus norvegicus 143-146 6098141-6 1984 In carbachol-contracted tracheas with maximally effective concentrations present of adenosine (3 mM), adenine (3 mM) or VIP (23.3 microM), the non-adrenergic neurogenic inhibitory response remained intact. Carbachol 3-12 VIP peptides Cavia porcellus 120-123 6149698-6 1984 Carbachol noncompetitively inhibited SLI secretion stimulated by gastrin, epinephrine, and dibutyryl cAMP. Carbachol 0-9 gastrin Canis lupus familiaris 65-72 6091776-4 1984 Carbachol produced a steady-state increase of [Ca2+]in and its effect was blocked by atropine and Ca2+ -channel blocking agents. Carbachol 0-9 carbonic anhydrase 2 Homo sapiens 98-101 6206899-1 1984 Acetylcholine, oxotremorine and carbachol, compounds that exhibit muscarinic agonist activity, maximally inhibited basal prolactin secretion from GH3 cells by approx. Carbachol 32-41 prolactin Rattus norvegicus 121-130 6499920-9 1984 In isolated guinea-pig atria UL-FS 49 antagonized the carbachol-induced bradycardia; a 10-fold shift of the dose-response curve (CA10) was achieved with 11.3 micrograms/ml and the CA10 for AQ-A 39 was 1.7 micrograms/ml. Carbachol 54-63 carbonic anhydrase-related protein 10 Cavia porcellus 129-133 6499920-9 1984 In isolated guinea-pig atria UL-FS 49 antagonized the carbachol-induced bradycardia; a 10-fold shift of the dose-response curve (CA10) was achieved with 11.3 micrograms/ml and the CA10 for AQ-A 39 was 1.7 micrograms/ml. Carbachol 54-63 carbonic anhydrase-related protein 10 Cavia porcellus 180-184 6148690-7 1984 A normal [3H]cyclic GMP response to bradykinin and histamine was demonstrated to be present in cells that had lost the [3H]cyclic GMP response to carbachol. Carbachol 146-155 5'-nucleotidase, cytosolic II Mus musculus 20-23 3886987-9 1985 Furthermore, the hypothyroid state developed in CCl4-treated rats may provide favorable conditions for the stimulation of DNA synthesis by isoproterenol and carbachol. Carbachol 157-166 C-C motif chemokine ligand 4 Rattus norvegicus 48-52 6436084-2 1984 In the presence of either divalent cationic chelator (EGTA) or calcium channel blocker (verapamil, nifedipine), carbachol-stimulated gastrin release was inhibited completely to values that were not significantly different from non-stimulated control. Carbachol 112-121 gastrin Rattus norvegicus 133-140 6436084-3 1984 In the absence of added calcium chloride, carbachol stimulated gastrin release during the initial 30 min of culture but not at 69 and 120 min of culture. Carbachol 42-51 gastrin Rattus norvegicus 63-70 6436084-4 1984 Inhibition by EGTA and verapamil of carbachol-stimulated gastrin release during the initial 30 min of culture suggests, but does not prove, that these agents may also effect intracellular availability and movement of calcium. Carbachol 36-45 gastrin Rattus norvegicus 57-64 6478213-5 1984 We propose that on H-type synapses detergents may perturb a hypothetical molecular interaction between AChE and the acetylcholine receptor (AChR) by which AChE modulates the ability of the AChR to be activated by ACh or carbachol. Carbachol 220-229 acetylcholinesterase (Cartwright blood group) Homo sapiens 103-107 6478213-5 1984 We propose that on H-type synapses detergents may perturb a hypothetical molecular interaction between AChE and the acetylcholine receptor (AChR) by which AChE modulates the ability of the AChR to be activated by ACh or carbachol. Carbachol 220-229 acetylcholinesterase (Cartwright blood group) Homo sapiens 155-159 6331450-1 1984 In neuroblastoma N1E 115 cells, carbachol, histamine and PGE1 elevated cyclic GMP content and, induced the efflux of preloaded 45Ca2+, the release of membrane-bound Ca2+ measured by fluorescent CTC, and the increase in [Ca2+]i as measured by Quin 2 fluorescence. Carbachol 32-41 5'-nucleotidase, cytosolic II Mus musculus 78-81 6144613-2 1984 The purpose of this study was to examine further the relationships between gastrin and somatostatin and the effects of the cholinergic agonist carbachol on content and release of gastrin and somatostatin using rat antral mucosa in tissue culture. Carbachol 143-152 gastrin Rattus norvegicus 179-186 6144613-2 1984 The purpose of this study was to examine further the relationships between gastrin and somatostatin and the effects of the cholinergic agonist carbachol on content and release of gastrin and somatostatin using rat antral mucosa in tissue culture. Carbachol 143-152 somatostatin Rattus norvegicus 191-203 6144613-5 1984 Inclusion of carbachol 2.5 X 10(-6) M in the culture medium decreased medium somatostatin from 1.91 +/- 0.28 (SEM) ng/mg tissue protein to 0.62 +/- 0.12 ng/mg (p less than 0.01), extracted mucosal somatostatin from 2.60 +/- 0.30 to 1.52 +/- 0.16 ng/mg (p less than 0.001), and percentage of somatostatin released from 42% +/- 2.6% to 27% +/- 2.2% (p less than 0.01). Carbachol 13-22 somatostatin Rattus norvegicus 77-89 6144613-5 1984 Inclusion of carbachol 2.5 X 10(-6) M in the culture medium decreased medium somatostatin from 1.91 +/- 0.28 (SEM) ng/mg tissue protein to 0.62 +/- 0.12 ng/mg (p less than 0.01), extracted mucosal somatostatin from 2.60 +/- 0.30 to 1.52 +/- 0.16 ng/mg (p less than 0.001), and percentage of somatostatin released from 42% +/- 2.6% to 27% +/- 2.2% (p less than 0.01). Carbachol 13-22 somatostatin Rattus norvegicus 197-209 6144613-5 1984 Inclusion of carbachol 2.5 X 10(-6) M in the culture medium decreased medium somatostatin from 1.91 +/- 0.28 (SEM) ng/mg tissue protein to 0.62 +/- 0.12 ng/mg (p less than 0.01), extracted mucosal somatostatin from 2.60 +/- 0.30 to 1.52 +/- 0.16 ng/mg (p less than 0.001), and percentage of somatostatin released from 42% +/- 2.6% to 27% +/- 2.2% (p less than 0.01). Carbachol 13-22 somatostatin Rattus norvegicus 197-209 6144613-6 1984 Carbachol also increased culture media gastrin from 14 +/- 2.5 to 27 +/- 3.0 ng/mg protein (p less than 0.01). Carbachol 0-9 gastrin Rattus norvegicus 39-46 6144613-8 1984 Incubation with somatostatin antisera, both with and without carbachol, markedly increased culture media concentrations of somatostatin, all of which was effectively bound by antibodies present in the media. Carbachol 61-70 somatostatin Rattus norvegicus 123-135 6144613-9 1984 Antibody binding of somatostatin was accompanied by significant increases in culture media gastrin concentrations, both in the presence and in the absence of carbachol. Carbachol 158-167 somatostatin Rattus norvegicus 20-32 6326613-5 1984 In addition, EGF was found to suppress H+ formation in the isolated gastric glands, both under resting conditions and after stimulation with histamine, carbachol, or dibutyryl cAMP. Carbachol 152-161 pro-epidermal growth factor Oryctolagus cuniculus 13-16 6088825-7 1984 These results indicate that carbachol injected intracerebroventricularly produces vasopressor effects mainly by releasing pituitary hormones, probably vasopressin, and that augmented pressor responses in SHR may be due to excessive release of vasopressin. Carbachol 28-37 arginine vasopressin Rattus norvegicus 151-162 6088825-7 1984 These results indicate that carbachol injected intracerebroventricularly produces vasopressor effects mainly by releasing pituitary hormones, probably vasopressin, and that augmented pressor responses in SHR may be due to excessive release of vasopressin. Carbachol 28-37 arginine vasopressin Rattus norvegicus 243-254 6200615-9 1984 Contractions evoked by transmural electric field stimulation or pharmacologically with carbachol, noradrenaline, substance P and prostaglandin F2 alpha were equally reduced by VIP 10(-7) mol. Carbachol 87-96 vasoactive intestinal peptide Sus scrofa 176-179 6091414-0 1984 Calcium-dependent enhancement by carbachol of the VIP-induced cyclic AMP accumulation in cat submandibular gland. Carbachol 33-42 vasoactive intestinal peptide Homo sapiens 50-53 6091414-2 1984 Carbachol was found to potentiate the cyclic AMP increase induced by VIP by an atropine sensitive mechanism. Carbachol 0-9 vasoactive intestinal peptide Homo sapiens 69-72 6091414-3 1984 The effect of carbachol on cyclic AMP accumulation was abolished by including EGTA in the incubation medium as was the carbachol mediated potentiation of VIP responses. Carbachol 14-23 vasoactive intestinal peptide Homo sapiens 154-157 6091414-3 1984 The effect of carbachol on cyclic AMP accumulation was abolished by including EGTA in the incubation medium as was the carbachol mediated potentiation of VIP responses. Carbachol 119-128 vasoactive intestinal peptide Homo sapiens 154-157 6091414-6 1984 The phospholipase A2 inhibitor, mepacrine, tended to decrease carbachol actions. Carbachol 62-71 phospholipase A2 group IB Homo sapiens 4-20 6322925-2 1984 Carbachol microinjection into an area surrounding the lateral half of the brachium conjunctivum (parabrachial region, PBR) produced profound suppression of nociceptive responses. Carbachol 0-9 translocator protein Homo sapiens 118-121 6322925-4 1984 Carbachol microinjection into the ventral part of PBR resulted in slight suppression of motor responses in addition to profound nociceptive suppression. Carbachol 0-9 translocator protein Homo sapiens 50-53 6322925-5 1984 Carbachol-produced analgesia (CPA) observed within PBR blocked supraspinally as well as spinally integrated responses normally elicited by either phasic or tonic noxious stimuli. Carbachol 0-9 translocator protein Homo sapiens 51-54 6328953-2 1984 In the rat, IF was localized to the chief cells and its secretion responded most efficaciously to carbachol. Carbachol 98-107 cobalamin binding intrinsic factor Rattus norvegicus 12-14 6328953-4 1984 In man, IF secretion derived from the parietal cells and was increasingly enhanced by hexoprenaline, pentagastrin, carbachol, histamine and dbcAMP. Carbachol 115-124 cobalamin binding intrinsic factor Rattus norvegicus 8-10 6200315-1 1984 It has been previously shown that carbamylcholine (10(-5) M) decreases TSH-induced cAMP accumulation and hormone secretion in dog thyroid slices. Carbachol 34-49 cathelicidin antimicrobial peptide Canis lupus familiaris 83-87 6200315-10 1984 It is concluded that carbamylcholine (10(-5) M) inhibits stimulated thyroid secretion at a step beyond cAMP accumulation by blocking pseudopod formation and not by inhibiting thyroglobulin hydrolysis or hormone diffusion. Carbachol 21-36 cathelicidin antimicrobial peptide Canis lupus familiaris 103-107 6204039-5 1984 VIP, IBMX and 8-Br-cyclic AMP, all of which act through or mimic the action of cyclic AMP, potentiated the secretory response to maximal concentrations of CCK, carbamylcholine and the ionophore A23187, all of which act via intracellular calcium. Carbachol 160-175 vasoactive intestinal polypeptide Mus musculus 0-3 6584029-4 1984 Similarly, in long-term (10-day) monolayer cultures of cells from four corpora lutea, human chorionic gonadotropin (hCG) (50 ng/ml) and PGE2 stimulated, but none of the adrenergic or cholinergic agents altered, the production of progesterone significantly, except for an inhibitory effect of norepinephrine and carbachol in the presence of 17 beta-estradiol (10(-7)M) added to the culture medium. Carbachol 311-320 chorionic gonadotropin subunit beta 5 Homo sapiens 92-120 6148690-7 1984 A normal [3H]cyclic GMP response to bradykinin and histamine was demonstrated to be present in cells that had lost the [3H]cyclic GMP response to carbachol. Carbachol 146-155 5'-nucleotidase, cytosolic II Mus musculus 130-133 6147816-5 1984 Addition of carbachol (3 microM) potentiated the effects of both secretin and VIP on TH activity. Carbachol 12-21 secretin Homo sapiens 65-73 6148102-9 1984 The activity of the plasma membrane-bound guanylate cyclase was about 10-fold enhanced by the nonionic detergent Triton X-100 and high concentrations of lysophosphatidylcholine, but was slightly decreased upon addition of the alpha-cholinergic agonist carbachol. Carbachol 252-261 guanylate cyclase Bos taurus 42-59 6322784-3 1984 Two lines of evidence suggest that this effect of cholecystokinin on basal and insulin-stimulated 125I-IGF II binding was mediated via a change in intracellular calcium: (1) the cholinergic agent carbachol inhibited IGF II binding to its receptors; (2) addition of the Ca2+ ionophore A23187 mimicked the effects of CCK8 and carbachol. Carbachol 196-205 insulin-like growth factor 2 Mus musculus 103-109 6322784-3 1984 Two lines of evidence suggest that this effect of cholecystokinin on basal and insulin-stimulated 125I-IGF II binding was mediated via a change in intracellular calcium: (1) the cholinergic agent carbachol inhibited IGF II binding to its receptors; (2) addition of the Ca2+ ionophore A23187 mimicked the effects of CCK8 and carbachol. Carbachol 196-205 insulin-like growth factor 2 Mus musculus 216-222 6714311-1 1984 Vasoactive intestinal peptide (VIP) relaxed isolated guinea pig airways contracted with carbamylcholine and isolated pulmonary arteries contracted with prostaglandin F2 alpha. Carbachol 88-103 VIP peptides Cavia porcellus 0-29 6714311-1 1984 Vasoactive intestinal peptide (VIP) relaxed isolated guinea pig airways contracted with carbamylcholine and isolated pulmonary arteries contracted with prostaglandin F2 alpha. Carbachol 88-103 VIP peptides Cavia porcellus 31-34 6322934-0 1984 Increase of carbamylcholine-induced 22Na+ influx into pheochromocytoma PC12h cells by nerve growth factor. Carbachol 12-27 nerve growth factor Rattus norvegicus 86-105 6322934-1 1984 Carbamylcholine (CCh)-induced 22Na+ influx into clonal rat pheochromocytoma PC12h cells increased remarkably by culturing the cells in the presence of nerve growth factor (NGF) at a concentration of 50 ng/ml. Carbachol 0-15 nerve growth factor Rattus norvegicus 151-170 6322934-1 1984 Carbamylcholine (CCh)-induced 22Na+ influx into clonal rat pheochromocytoma PC12h cells increased remarkably by culturing the cells in the presence of nerve growth factor (NGF) at a concentration of 50 ng/ml. Carbachol 0-15 nerve growth factor Rattus norvegicus 172-175 6322934-1 1984 Carbamylcholine (CCh)-induced 22Na+ influx into clonal rat pheochromocytoma PC12h cells increased remarkably by culturing the cells in the presence of nerve growth factor (NGF) at a concentration of 50 ng/ml. Carbachol 17-20 nerve growth factor Rattus norvegicus 151-170 6322934-1 1984 Carbamylcholine (CCh)-induced 22Na+ influx into clonal rat pheochromocytoma PC12h cells increased remarkably by culturing the cells in the presence of nerve growth factor (NGF) at a concentration of 50 ng/ml. Carbachol 17-20 nerve growth factor Rattus norvegicus 172-175 6322934-2 1984 After 2-4 days in culture with NGF, the CCh-induced 22Na+ influx into cells was enhanced 3- to 5-fold compared to the influx into NGF-untreated cells. Carbachol 40-43 nerve growth factor Rattus norvegicus 31-34 6322934-2 1984 After 2-4 days in culture with NGF, the CCh-induced 22Na+ influx into cells was enhanced 3- to 5-fold compared to the influx into NGF-untreated cells. Carbachol 40-43 nerve growth factor Rattus norvegicus 130-133 6322934-5 1984 Besides NGF, epidermal growth factor increased the CCh-induced 22Na+ influx into cells to a lower extent that NGF did. Carbachol 51-54 nerve growth factor Rattus norvegicus 8-11 6694115-6 1984 However, in rats infused with carbachol for 2 or 5 days, the vasopressin levels were not significantly different from controls. Carbachol 30-39 arginine vasopressin Rattus norvegicus 61-72 6694115-11 1984 injections of carbachol were due to increased sympathetic activity and vasopressin release. Carbachol 14-23 arginine vasopressin Rattus norvegicus 71-82 6147816-5 1984 Addition of carbachol (3 microM) potentiated the effects of both secretin and VIP on TH activity. Carbachol 12-21 vasoactive intestinal peptide Homo sapiens 78-81 6139724-2 1983 In two of the cell lines (DMS 53, DMS 153) acetylcholine chloride, bethanechol chloride, and carbamylcholine at the concentrations of 10(-3)M to 10(-5)M stimulated secretion of bombesin and calcitonin as measured by RIA. Carbachol 93-108 gastrin releasing peptide Homo sapiens 177-185 6394244-11 1984 When intact sheets of epithelium were isolated from adult rat ileum and colon, then maintained in vitro and exposed to 20 microM-carbachol, crypt goblet cells released mucin in response to the secretagogue but goblet cells in in portions of the epithelium derived from villi or mucosal surfaces were unresponsive. Carbachol 129-138 solute carrier family 13 member 2 Rattus norvegicus 168-173 6418215-7 1983 Carbachol (1 X 10(-6)-10(-3) mol/l) stimulated intrinsic factor secretion, 1 X 10(-3) mol/l being maximally effective (90 +/- 8% above basal). Carbachol 0-9 cobalamin binding intrinsic factor Rattus norvegicus 47-63 6197390-2 1983 SP contracted the sphincter and the maximum response was only about 25% of that in the presence of equimolar doses of carbachol. Carbachol 118-127 tachykinin precursor 1 Bos taurus 0-2 6138366-2 1983 The present studies were directed to examine the effect of secretin on carbachol-stimulated gastrin release and to assess the potential role of somatostatin in mediating this effect. Carbachol 71-80 secretin Rattus norvegicus 59-67 6138366-2 1983 The present studies were directed to examine the effect of secretin on carbachol-stimulated gastrin release and to assess the potential role of somatostatin in mediating this effect. Carbachol 71-80 gastrin Rattus norvegicus 92-99 6138366-5 1983 Carbachol (2.5 X 10(-6) M) in the culture medium increased gastrin level in the medium from 14.1 +/- 2.5 to 26.9 +/- 3.0 ng/mg tissue protein (P less than 0.02), and decreased somatostatin-like immunoreactivity in the medium from 1.91 +/- 0.28 to 0.62 +/- 0.12 ng/mg (P less than 0.01) and extracted mucosal somatostatin-like immunoreactivity from 2.60 +/- 0.30 to 1.52 +/- 0.16 ng/mg (P less than 0.001). Carbachol 0-9 gastrin Rattus norvegicus 59-66 6138366-6 1983 Rat antral mucosa was then cultured in the presence of secretin to determine its effect on carbachol-stimulated gastrin release. Carbachol 91-100 secretin Rattus norvegicus 55-63 6138366-6 1983 Rat antral mucosa was then cultured in the presence of secretin to determine its effect on carbachol-stimulated gastrin release. Carbachol 91-100 gastrin Rattus norvegicus 112-119 6138366-7 1983 Inclusion of secretin (10(-9)-10(-7) M) inhibited significantly carbachol-stimulated gastrin release into the medium, decreasing gastrin from 26.9 +/- 3.0 to 13.6 +/- 3.2 ng/mg (10(-9) M secretin) (P less than 0.05), to 11.9 +/- 1.7 ng/mg (10(-8) secretin) (P less than 0.02), and to 10.8 +/- 4.0 ng/mg (10(-7) M secretin) (P less than 0.02). Carbachol 64-73 secretin Rattus norvegicus 13-21 6138366-7 1983 Inclusion of secretin (10(-9)-10(-7) M) inhibited significantly carbachol-stimulated gastrin release into the medium, decreasing gastrin from 26.9 +/- 3.0 to 13.6 +/- 3.2 ng/mg (10(-9) M secretin) (P less than 0.05), to 11.9 +/- 1.7 ng/mg (10(-8) secretin) (P less than 0.02), and to 10.8 +/- 4.0 ng/mg (10(-7) M secretin) (P less than 0.02). Carbachol 64-73 gastrin Rattus norvegicus 85-92 6138366-7 1983 Inclusion of secretin (10(-9)-10(-7) M) inhibited significantly carbachol-stimulated gastrin release into the medium, decreasing gastrin from 26.9 +/- 3.0 to 13.6 +/- 3.2 ng/mg (10(-9) M secretin) (P less than 0.05), to 11.9 +/- 1.7 ng/mg (10(-8) secretin) (P less than 0.02), and to 10.8 +/- 4.0 ng/mg (10(-7) M secretin) (P less than 0.02). Carbachol 64-73 gastrin Rattus norvegicus 129-136 6138366-7 1983 Inclusion of secretin (10(-9)-10(-7) M) inhibited significantly carbachol-stimulated gastrin release into the medium, decreasing gastrin from 26.9 +/- 3.0 to 13.6 +/- 3.2 ng/mg (10(-9) M secretin) (P less than 0.05), to 11.9 +/- 1.7 ng/mg (10(-8) secretin) (P less than 0.02), and to 10.8 +/- 4.0 ng/mg (10(-7) M secretin) (P less than 0.02). Carbachol 64-73 secretin Rattus norvegicus 187-195 6138366-7 1983 Inclusion of secretin (10(-9)-10(-7) M) inhibited significantly carbachol-stimulated gastrin release into the medium, decreasing gastrin from 26.9 +/- 3.0 to 13.6 +/- 3.2 ng/mg (10(-9) M secretin) (P less than 0.05), to 11.9 +/- 1.7 ng/mg (10(-8) secretin) (P less than 0.02), and to 10.8 +/- 4.0 ng/mg (10(-7) M secretin) (P less than 0.02). Carbachol 64-73 secretin Rattus norvegicus 187-195 6138366-7 1983 Inclusion of secretin (10(-9)-10(-7) M) inhibited significantly carbachol-stimulated gastrin release into the medium, decreasing gastrin from 26.9 +/- 3.0 to 13.6 +/- 3.2 ng/mg (10(-9) M secretin) (P less than 0.05), to 11.9 +/- 1.7 ng/mg (10(-8) secretin) (P less than 0.02), and to 10.8 +/- 4.0 ng/mg (10(-7) M secretin) (P less than 0.02). Carbachol 64-73 secretin Rattus norvegicus 187-195 6138366-9 1983 To determine whether secretion inhibition of carbachol-stimulated gastrin release was mediated by somatostatin, antral mucosa was cultured with carbachol, secretin (10(-9)-10(-7) M), and antibodies to somatostatin. Carbachol 45-54 gastrin Rattus norvegicus 66-73 6138366-10 1983 Inclusion of somatostatin antibodies in the culture medium abolished the capacity of secretin (10(-7) and 10(-8) M) to inhibit carbachol-stimulated gastrin release. Carbachol 127-136 secretin Rattus norvegicus 85-93 6138366-10 1983 Inclusion of somatostatin antibodies in the culture medium abolished the capacity of secretin (10(-7) and 10(-8) M) to inhibit carbachol-stimulated gastrin release. Carbachol 127-136 gastrin Rattus norvegicus 148-155 6138366-11 1983 Results of these studies indicate (a) that secretin inhibits carbachol-stimulated gastrin release and (b) that under the conditions of these experiments secretin inhibition of gastrin release is mediated, at least in part, locally through release of antral somatostatin. Carbachol 61-70 secretin Rattus norvegicus 43-51 6138366-11 1983 Results of these studies indicate (a) that secretin inhibits carbachol-stimulated gastrin release and (b) that under the conditions of these experiments secretin inhibition of gastrin release is mediated, at least in part, locally through release of antral somatostatin. Carbachol 61-70 gastrin Rattus norvegicus 82-89 6137958-5 1983 Bombesin and carbachol both evoked a dose-dependent stimulation of gastrin release, beginning at below 10(-10) M (bombesin) and 10(-7) M (carbachol). Carbachol 13-22 gastrin Homo sapiens 67-74 6137958-5 1983 Bombesin and carbachol both evoked a dose-dependent stimulation of gastrin release, beginning at below 10(-10) M (bombesin) and 10(-7) M (carbachol). Carbachol 13-22 gastrin releasing peptide Homo sapiens 114-122 6137958-5 1983 Bombesin and carbachol both evoked a dose-dependent stimulation of gastrin release, beginning at below 10(-10) M (bombesin) and 10(-7) M (carbachol). Carbachol 138-147 gastrin releasing peptide Homo sapiens 0-8 6137958-5 1983 Bombesin and carbachol both evoked a dose-dependent stimulation of gastrin release, beginning at below 10(-10) M (bombesin) and 10(-7) M (carbachol). Carbachol 138-147 gastrin Homo sapiens 67-74 6137958-6 1983 Carbachol inhibited the release of somatostatin in a dose-dependent manner, being maximally effective at 10(-6) M and then producing 60% inhibition of somatostatin release. Carbachol 0-9 somatostatin Homo sapiens 35-47 6137958-6 1983 Carbachol inhibited the release of somatostatin in a dose-dependent manner, being maximally effective at 10(-6) M and then producing 60% inhibition of somatostatin release. Carbachol 0-9 somatostatin Homo sapiens 151-163 6137958-8 1983 These data suggest that the gastrin-stimulating effect of carbachol is partially or totally due to inhibition of somatostatin release, whereas bombesinergic stimulation of gastrin release must work in an independent manner. Carbachol 58-67 gastrin Homo sapiens 28-35 6137958-8 1983 These data suggest that the gastrin-stimulating effect of carbachol is partially or totally due to inhibition of somatostatin release, whereas bombesinergic stimulation of gastrin release must work in an independent manner. Carbachol 58-67 somatostatin Homo sapiens 113-125 6197722-0 1983 Neurotensin interacts with carbachol, secretin, and caerulein in the stimulation of the exocrine pancreas of the rat in vitro. Carbachol 27-36 neurotensin Rattus norvegicus 0-11 6139157-9 1983 Activation of antral motility by stimulation of the abdominal vagus or intraarterial carbachol injections to the antrum increased duodenal IR motilin release in the absence of duodenal motility. Carbachol 85-94 motilin Canis lupus familiaris 142-149 6089131-5 1984 The secretory responses induced by carbachol (CCh) at any concentrations were not potentiated but inhibited by simultaneous stimulation of VIP. Carbachol 35-44 VIP peptides Cavia porcellus 139-142 6089131-5 1984 The secretory responses induced by carbachol (CCh) at any concentrations were not potentiated but inhibited by simultaneous stimulation of VIP. Carbachol 46-49 VIP peptides Cavia porcellus 139-142 6888186-3 1983 VIP in chromaffin cells in culture appears to be contained in a secretory granule pool, since it, like methionine-enkephalin (met-enk) was released into the medium after exposure of cells to nicotine, carbachol, veratridine and elevated potassium in a dose-dependent manner. Carbachol 201-210 vasoactive intestinal peptide Bos taurus 0-3 6309912-7 1983 The calmodulin antagonists, trifluoperazine (10(-5) M), pimozide (10(-5) M), and a naphthalenesulfonamide (W-7), inhibited the integrated response to histamine by 54, 56, and 53%, and that of carbamylcholine by 65, 64, and 99%, respectively. Carbachol 192-207 calmodulin-3 Cavia porcellus 4-14 6309912-9 1983 The action of carbamylcholine is completely dependent upon calcium/calmodulin mediation, supporting the concept that cholinergic actions are mediated via calcium-calmodulin events. Carbachol 14-29 calmodulin-3 Cavia porcellus 67-77 6309912-9 1983 The action of carbamylcholine is completely dependent upon calcium/calmodulin mediation, supporting the concept that cholinergic actions are mediated via calcium-calmodulin events. Carbachol 14-29 calmodulin-3 Cavia porcellus 162-172 6197124-1 1983 Propranolol-resistant neurogenic relaxation persisted in (carbachol-contracted) guinea-pig tracheae already relaxed by supramaximal concentrations of vasoactive intestinal polypeptide (VIP). Carbachol 58-67 VIP peptides Cavia porcellus 150-183 6193336-6 1983 Carbachol increased the activities of cathepsin D and amylase in ascites and acid phosphatase in pancreatic tissue. Carbachol 0-9 cathepsin D Rattus norvegicus 38-49 6197124-1 1983 Propranolol-resistant neurogenic relaxation persisted in (carbachol-contracted) guinea-pig tracheae already relaxed by supramaximal concentrations of vasoactive intestinal polypeptide (VIP). Carbachol 58-67 VIP peptides Cavia porcellus 185-188 6189522-7 1983 The inhibition of amylase secretion caused by the butyloxycarbonyl tripeptide of cholecystokinin was reversible and specific for those peptides which interact with the cholecystokinin receptor (i.e., cholecystokinin, caerulein, gastrin); it did not inhibit the actions of bombesin, carbachol, physalaemin, vasoactive intestinal peptide, secretin, PHI, ionophore A23187 or 8-bromo cyclic AMP. Carbachol 282-291 cholecystokinin Cavia porcellus 81-96 6318567-8 1983 Stimulation of pepsinogen secretion by carbachol or isoproterenol was not inhibited by DBcGMP nor was the stimulation of acid formation by CCK-like peptides. Carbachol 39-48 pepsin II-2/3 Oryctolagus cuniculus 15-25 6866010-4 1983 Of the four mcAbs studies only mcAb 5.5, which is directed against the cholinergic site in Torpedo AChR, blocks the binding of alpha-bungarotoxin (alpha-Bgt) to AChR in chick muscle cultures and inhibits carbamylcholine-induced sodium transport in these cells. Carbachol 204-219 cholinergic receptor nicotinic delta subunit Gallus gallus 99-103 6301290-1 1983 In dispersed glands from rabbit stomach, pepsinogen secretion was stimulated by carbamylcholine, the C-terminal octapeptide of cholecystokinin (CCK-8), and structurally related peptides physalaemin and A23187 but not by bombesin or histamine. Carbachol 80-95 pepsin II-2/3 Oryctolagus cuniculus 41-51 6301290-3 1983 Dibutyryl cGMP inhibited the stimulation of pepsinogen secretion caused by cholecystokinin but not that caused by carbamylcholine; atropine inhibited the stimulation of pepsinogen secretion caused by carbamylcholine but not that caused by cholecystokinin. Carbachol 200-215 pepsin II-2/3 Oryctolagus cuniculus 169-179 6847709-3 1983 Phospholipase A2 inhibitors, such as quinacrine, chloroquine, quinine and p-bromophenacyl bromide, all inhibited the secretion of catecholamines evoked by carbamylcholine in a dose-dependent manner. Carbachol 155-170 LOC104974671 Bos taurus 0-16 6847709-4 1983 These phospholipase A2 inhibitors also inhibited the cellular uptake of 45Ca2+ evoked by carbamylcholine with similar dose-response curves to those for inhibition of catecholamine secretion. Carbachol 89-104 LOC104974671 Bos taurus 6-22 6189522-7 1983 The inhibition of amylase secretion caused by the butyloxycarbonyl tripeptide of cholecystokinin was reversible and specific for those peptides which interact with the cholecystokinin receptor (i.e., cholecystokinin, caerulein, gastrin); it did not inhibit the actions of bombesin, carbachol, physalaemin, vasoactive intestinal peptide, secretin, PHI, ionophore A23187 or 8-bromo cyclic AMP. Carbachol 282-291 cholecystokinin Cavia porcellus 168-183 6189522-7 1983 The inhibition of amylase secretion caused by the butyloxycarbonyl tripeptide of cholecystokinin was reversible and specific for those peptides which interact with the cholecystokinin receptor (i.e., cholecystokinin, caerulein, gastrin); it did not inhibit the actions of bombesin, carbachol, physalaemin, vasoactive intestinal peptide, secretin, PHI, ionophore A23187 or 8-bromo cyclic AMP. Carbachol 282-291 cholecystokinin Cavia porcellus 168-183 6822533-2 1983 The use of two separate assays, specific binding of 125I-alpha-bungarotoxin and carbamylcholine-activated 22Na+ uptake, has allowed us to monitor the effects of impaired glycosylation on the metabolic and functional properties of AChR. Carbachol 80-95 cholinergic receptor nicotinic delta subunit Gallus gallus 230-234 6129701-1 1983 Somatostatin, a tetradecapeptide with potent inhibitory actions on gastric acid secretion, potentiated carbamylcholine-induced synthesis and release of prostaglandin E2 from isolated perfused rat stomachs. Carbachol 103-118 somatostatin Rattus norvegicus 0-12 6298631-3 1983 However, we report here that after inhibiting AChE in a cholinergic synapse in Aplysia, we found an increase not only in postsynaptic responses to presynaptic stimulation and to ionophoretic application of ACh on postsynaptic receptors, but also to ionophoretic application of carbachol. Carbachol 277-286 acetylcholinesterase (Cartwright blood group) Homo sapiens 46-50 6687224-6 1983 Carbachol bound to the A-promoter-treated membranes with a lower affinity and a higher Hill coefficient, and these kinetic values were not altered by Gpp(NH)p, indicating that treatment of membranes with the A-protomer of IAP uncoupled muscarinic receptors from N. This IAP-sensitive N is Ni involved in the cyclase inhibition. Carbachol 0-9 Cd47 molecule Rattus norvegicus 222-225 6309168-2 1983 Carbachol markedly decreased the stimulatory effect of the adenylate cyclase activator, forskolin, on both cyclic AMP formation and ACTH secretion. Carbachol 0-9 pro-opiomelanocortin-alpha Mus musculus 132-136 6309168-4 1983 The stimulatory effects of (-) isoproterenol on cyclic nucleotide formation and ACTH secretion were also blocked by carbachol. Carbachol 116-125 pro-opiomelanocortin-alpha Mus musculus 80-84 6309168-5 1983 The inhibitory effects of carbachol on (-) isoproterenol-stimulated cyclic AMP synthesis and ACTH secretion were reversed by the muscarinic antagonist, atropine, and not by the nicotinic antagonist, gallamine. Carbachol 26-35 pro-opiomelanocortin-alpha Mus musculus 93-97 6297650-2 1983 2 Sixteen of these compounds were assayed for their ability to antagonize carbachol-stimulated cyclic guanosine 3",5"-monophosphate (cyclic GMP) synthesis by intact murine neuroblastoma cells (clone N1E-115). Carbachol 74-83 5'-nucleotidase, cytosolic II Homo sapiens 140-143 6687224-6 1983 Carbachol bound to the A-promoter-treated membranes with a lower affinity and a higher Hill coefficient, and these kinetic values were not altered by Gpp(NH)p, indicating that treatment of membranes with the A-protomer of IAP uncoupled muscarinic receptors from N. This IAP-sensitive N is Ni involved in the cyclase inhibition. Carbachol 0-9 Cd47 molecule Rattus norvegicus 270-273 6402798-3 1983 Exposure to carbamylcholine, veratridine or high K evokes a transient increase in the rate of catecholamine release in association with dopamine beta hydroxylase but not lactate dehydrogenase. Carbachol 12-27 dopamine beta-hydroxylase Bos taurus 136-161 6184589-4 1982 The phosphodiesterase inhibitor 3-isobutyl-1-methyl-xanthine (MIX) enhanced the effects of carbachol on both c-GMP accumulation and amylase release. Carbachol 91-100 5'-nucleotidase, cytosolic II Mus musculus 111-114 6127400-9 1982 It is concluded that tyrosine 3-monooxygenase activity in rat superior cervical ganglia can be increased by both nicotinic and muscarinic stimulation, that nicotinic stimulation can produce a greater increase than can muscarinic stimulation and that carbachol increases enzyme activity by a combination of both pathways. Carbachol 250-259 tyrosine hydroxylase Rattus norvegicus 21-45 7144436-2 1982 Its activity was demonstrated orally at low dose in pentagastrin stimulated Shay rat and in Heidenhain pouch in dog against gastrin, pentagastrin, carbachol and test meal. Carbachol 147-156 gastrin Canis lupus familiaris 57-64 6287866-3 1982 Spontaneous release of pepsinogen was found to be less than 1% of the total per hour and relatively constant for at least 2 h. Pepsinogen release was stimulated in a dose-dependent manner by both carbachol and isoproterenol. Carbachol 196-205 pepsin II-2/3 Oryctolagus cuniculus 23-33 6287866-3 1982 Spontaneous release of pepsinogen was found to be less than 1% of the total per hour and relatively constant for at least 2 h. Pepsinogen release was stimulated in a dose-dependent manner by both carbachol and isoproterenol. Carbachol 196-205 pepsin II-2/3 Oryctolagus cuniculus 127-137 6818682-4 1982 In two subjects the effect of intravenously injected TRH, 200 micrograms, and atropine, 500 micrograms, on the carbacholine-stimulated gastric motility was tested. Carbachol 111-123 thyrotropin releasing hormone Homo sapiens 53-56 6818682-8 1982 The inhibiting effect of TRH on the carbacholine-stimulated gastric motility was similar to the effect of TRH on the hypoglycemia-stimulated gastric motility. Carbachol 36-48 thyrotropin releasing hormone Homo sapiens 25-28 6277122-8 1981 CCK-39 potentiated glucose- as well as carbachol-induced insulin secretion, whereas it did not influence L-IPNA-induced insulin release. Carbachol 39-48 cholecystokinin Mus musculus 0-3 6124857-2 1982 In the absence of extracellular sodium isoproterenol-stimulated c-GMP and c-AMP levels were significantly reduced; carbachol-stimulated c-GMP levels were not affected. Carbachol 115-124 5'-nucleotidase, cytosolic II Mus musculus 138-141 6177251-4 1982 Concentrations of cholecystokinin that were supramaximal for stimulating enzyme secretion abolished the stimulation caused by other secretagogues that promote mobilization of cellular calcium (e.g., carbamylcholine, bombesin, physalaemin, or A23187), as well as that caused by secretagogues that elevate cellular cAMP (e.g., vasoactive intestinal peptide or secretin). Carbachol 199-214 cholecystokinin Homo sapiens 18-33 6177536-0 1982 Carbachol potentiates the cyclic AMP-stimulating effect of VIP in cat submandibular gland. Carbachol 0-9 VIP peptides Cavia porcellus 59-62 6177536-4 1982 The addition of carbachol potentiated the cyclic AMP response to VIP in the submandibular acini while no such effect was observed in the pancreas. Carbachol 16-25 VIP peptides Cavia porcellus 65-68 6175231-3 1982 Carbamylcholine and the C-terminal octapeptide of cholecystokinin also augmented the action of VIP on amylase secretion from mouse pancreatic acini. Carbachol 0-15 vasoactive intestinal polypeptide Mus musculus 95-98 6175231-2 1982 Secretagogues that mobilize cellular calcium (carbamylcholine, C-terminal octapeptide of cholecystokinin, bombesin, or A23187) caused a sevenfold augmentation of the actions of VIP, secretin, or 8-bromo-cAMP on enzyme secretion. Carbachol 46-61 vasoactive intestinal peptide Rattus norvegicus 177-180 6175231-2 1982 Secretagogues that mobilize cellular calcium (carbamylcholine, C-terminal octapeptide of cholecystokinin, bombesin, or A23187) caused a sevenfold augmentation of the actions of VIP, secretin, or 8-bromo-cAMP on enzyme secretion. Carbachol 46-61 secretin Rattus norvegicus 182-190 6193153-0 1982 The role of cyclic nucleotide phosphodiesterase in the inhibition of cyclic AMP accumulation by carbachol and phosphatidate. Carbachol 96-105 phosphodiesterase 3B Homo sapiens 12-47 6289369-5 1982 Epinephrine and carbachol increased plasma cyclic AMP and cyclic GMP levels, respectively, in both C57BL and DBA mice. Carbachol 16-25 5'-nucleotidase, cytosolic II Mus musculus 65-68 6171823-4 1981 In contrast, stimuliation by carbachol induced significant phosphorylation of only protein I. Carbachol 29-38 annexin A2 Mus musculus 83-92 6171823-7 1981 Incubation of mouse parotid gland slices with either 20 microM isoproterenol or 10 microM carbachol resulted in strong and comparable releases of amylase, which were accompanied by comparable phosphorylations of protein I. Carbachol 90-99 annexin A2 Mus musculus 212-221 6171823-9 1981 Removal of external calcium by ethylene glycol bis(beta-aminoethyl ether)-N,N,N",N"-tetraacetate abolished the carbachol-induced release of amylase but not the phosphorylation of protein I. Carbachol 111-120 annexin A2 Mus musculus 179-188 7127212-6 1982 Atropine eliminated carbachol-induced I gastrin release and motility increases, even in the presence of nerve blockade by tetrodotoxin. Carbachol 20-29 gastrin Canis lupus familiaris 40-47 6174546-1 1981 After chemical stimulation with depolarizing agents (Ba2+ or Ca2+/carbachol) isolated living chromaffin cells display a drastically increased binding capacity for anti-DBH, distributed spotwise on or near the outer cell membrane. Carbachol 66-75 dopamine beta-hydroxylase Homo sapiens 168-171 6114896-0 1981 Somatostatin inhibition of basal and carbachol-stimulated gastrin release in rat antral organ culture. Carbachol 37-46 gastrin Rattus norvegicus 58-65 6114896-4 1981 Gastrin release into the culture media stimulated by the cholinergic agent, carbachol (10(-5) M), was suppressed by somatostatin: at 30 min and 6 h of culture 10(-8) M somatostatin inhibited carbachol-stimulated gastrin release by 66% and 54%, respectively, and 10(-5) M and 10(-4) M somatostatin completely abolished gastrin release. Carbachol 76-85 gastrin Rattus norvegicus 0-7 6114896-4 1981 Gastrin release into the culture media stimulated by the cholinergic agent, carbachol (10(-5) M), was suppressed by somatostatin: at 30 min and 6 h of culture 10(-8) M somatostatin inhibited carbachol-stimulated gastrin release by 66% and 54%, respectively, and 10(-5) M and 10(-4) M somatostatin completely abolished gastrin release. Carbachol 76-85 gastrin Rattus norvegicus 212-219 6114896-4 1981 Gastrin release into the culture media stimulated by the cholinergic agent, carbachol (10(-5) M), was suppressed by somatostatin: at 30 min and 6 h of culture 10(-8) M somatostatin inhibited carbachol-stimulated gastrin release by 66% and 54%, respectively, and 10(-5) M and 10(-4) M somatostatin completely abolished gastrin release. Carbachol 76-85 gastrin Rattus norvegicus 318-325 6114896-4 1981 Gastrin release into the culture media stimulated by the cholinergic agent, carbachol (10(-5) M), was suppressed by somatostatin: at 30 min and 6 h of culture 10(-8) M somatostatin inhibited carbachol-stimulated gastrin release by 66% and 54%, respectively, and 10(-5) M and 10(-4) M somatostatin completely abolished gastrin release. Carbachol 191-200 gastrin Rattus norvegicus 0-7 6114896-5 1981 The rate of gastrin release stimulated by carbachol was suppressed significantly by each dose of somatostatin examined (10(-8) M to 10(-4) M). Carbachol 42-51 gastrin Rattus norvegicus 12-19 6117505-0 1981 Inhibition by somatostatin of carbamylcholine-induced gastrin and glucagon release from the isolated perfused canine stomach. Carbachol 30-45 gastrin Canis lupus familiaris 54-61 6117505-1 1981 At an arterial plasma concentration of 61 nmol/l (100 ng/ml) synthetic cyclic somatostatin completely abolished basal glucagon and gastrin release as well as carbamylcholine-induced glucagon and gastrin release from the isolated perfused dog stomach. Carbachol 158-173 gastrin Canis lupus familiaris 195-202 6268193-10 1981 Cimetidine, a histamine H2 receptor antagonist, blocked the [14C]aminopyrine uptake induced either by histamine alone or by the potentiating combination of histamine plus carbachol. Carbachol 171-180 histamine H2 receptor Cavia porcellus 14-35 6117361-6 1981 intravenous infusion of carbachol (1 microgram.kg-1.min-1) strongly stimulated luminal somatostatin and gastrin release (from 5 +/- 1 to 192 +/- 52 pg.mL-1 and from 27 +/- 5 to 198 +/- 41 pg.mL-1, respectively) during perfusion with 0.1 M HCl. Carbachol 24-33 somatostatin Rattus norvegicus 87-99 6791509-6 1981 Carbachol mimicked both the stimulatory and inhibitory effects of CCK, whereas the Ca2+ ionophore A23187 mimicked only the inhibitory effects. Carbachol 0-9 cholecystokinin Rattus norvegicus 66-69 6170412-5 1981 Chlorpromazine, trifluoperazine, thioridazine, chlorprothixene and amitriptyline inhibited amylase secretion stimulated by carbachol, A-23187, and cholecystokinin-pancreozymin but not that elicited by dibutyryl cyclic AMP secretin or vasoactive intestinal peptide (VIP). Carbachol 123-132 vasoactive intestinal peptide Homo sapiens 265-268 7223893-7 1981 The data are interpreted to support our assumption that CCK-OP and CCh increase the plasma membrane permeability to Ca2+ in pancreatic acinar cells in addition to their action to initiate release of CA2+ from an intracellular Ca2+ trigger pool. Carbachol 67-70 carbonic anhydrase 2 Rattus norvegicus 116-119 6117361-6 1981 intravenous infusion of carbachol (1 microgram.kg-1.min-1) strongly stimulated luminal somatostatin and gastrin release (from 5 +/- 1 to 192 +/- 52 pg.mL-1 and from 27 +/- 5 to 198 +/- 41 pg.mL-1, respectively) during perfusion with 0.1 M HCl. Carbachol 24-33 gastrin Rattus norvegicus 104-111 6110537-10 1981 Plasma insulin and glucagon rose promptly after carbachol and were unchanged by atropine. Carbachol 48-57 insulin Canis lupus familiaris 7-14 6109792-5 1981 Because high carbachol concentrations were needed to produce gastrin secretion, and inhibition by atropine occurred also at high concentrations, these effects may be nonspecific. Carbachol 13-22 gastrin Rattus norvegicus 61-68 6109792-6 1981 Both carbachol- and norepinephrine-mediated gastrin release are modified by somatostatin and adenosine. Carbachol 5-14 gastrin Rattus norvegicus 44-51 7342828-0 1981 Feeding and growth hormone after cerebroventricular carbachol in sheep. Carbachol 52-61 somatotropin Ovis aries 12-26 7223893-7 1981 The data are interpreted to support our assumption that CCK-OP and CCh increase the plasma membrane permeability to Ca2+ in pancreatic acinar cells in addition to their action to initiate release of CA2+ from an intracellular Ca2+ trigger pool. Carbachol 67-70 carbonic anhydrase 2 Rattus norvegicus 199-202 7223893-7 1981 The data are interpreted to support our assumption that CCK-OP and CCh increase the plasma membrane permeability to Ca2+ in pancreatic acinar cells in addition to their action to initiate release of CA2+ from an intracellular Ca2+ trigger pool. Carbachol 67-70 carbonic anhydrase 2 Rattus norvegicus 226-229 6159206-8 1980 Carbamylcholine and the ionophore A23187, which can raise cGMP in thyroid slices, inhibited TSH and dibutyryl cAMP induced ODC increases, PGF1 alpha was inhibitory to ODC stimulation. Carbachol 0-15 ornithine decarboxylase 1 Canis lupus familiaris 123-126 7008812-3 1980 Carbamylcholine has been injected together with captopril, an inhibitor of the enzyme converting angiotensin I into angiotensin II, and with propranolol, which blocks the renin beta-receptors respectively. Carbachol 0-15 angiotensinogen Rattus norvegicus 116-130 7008812-3 1980 Carbamylcholine has been injected together with captopril, an inhibitor of the enzyme converting angiotensin I into angiotensin II, and with propranolol, which blocks the renin beta-receptors respectively. Carbachol 0-15 renin Rattus norvegicus 171-176 7009286-5 1981 Secretin inhibited insulin secretion induced by carbachol and L-IPNA, whereas VIP potentiated L-IPNA-induced insulin secretion and had no influence on the effect of carbachol. Carbachol 48-57 secretin Mus musculus 0-8 6109003-5 1980 Carbamylcholine-, ionophore X-537A-, and sodium azide-induced cyclic GMP formation increased with time in culture to a maximum of 13-, 9-, and 2.5-fold above basal, respectively. Carbachol 0-15 5'-nucleotidase, cytosolic II Mus musculus 69-72 6159206-8 1980 Carbamylcholine and the ionophore A23187, which can raise cGMP in thyroid slices, inhibited TSH and dibutyryl cAMP induced ODC increases, PGF1 alpha was inhibitory to ODC stimulation. Carbachol 0-15 ornithine decarboxylase 1 Canis lupus familiaris 167-170 7380199-1 1980 The purpose of the present study was to examine the effects of the cholinergic agent carbachol on gastrin synthesis and secretion by rat antral mucosa in organ culture. Carbachol 85-94 gastrin Rattus norvegicus 98-105 7394323-3 1980 Carbachol-contracted airway smooth muscle relaxed to PGE1, PGE2, isoproterenol, dimaprit (H2-agonist) and bradykinin (BK). Carbachol 0-9 kininogen 1 Canis lupus familiaris 106-116 7394323-3 1980 Carbachol-contracted airway smooth muscle relaxed to PGE1, PGE2, isoproterenol, dimaprit (H2-agonist) and bradykinin (BK). Carbachol 0-9 kininogen 1 Canis lupus familiaris 118-120 6245588-2 1980 Each enterocyte possessed approximately 60,000 binding sites and binding of the tracer to these sites could be inhibited by VIP [concentration for half-maximal effect (Kd), 12 nM] and by secretin (Kd greater than 1 micro M), but not by glucagon, gastrin, cholecystokinin, calcitonin, bombesin, litorin, physalaemin, substance P, eledoisin, serotonin, carbamylcholine, or histamine. Carbachol 351-366 VIP peptides Cavia porcellus 124-127 7380199-2 1980 In addition, the effect of atropine on basal and carbachol-stimulated gastrin release was investigated. Carbachol 49-58 gastrin Rattus norvegicus 70-77 7380199-3 1980 These in vitro studies demonstrate that carbachol stimulated both gastrin secretion and synthesis in a dose-dependent manner. Carbachol 40-49 gastrin Rattus norvegicus 66-73 7380199-4 1980 Maximal stimulation of gastrin synthesis and release occurred at a concentration of 1 X 10(-5) M carbachol. Carbachol 97-106 gastrin Rattus norvegicus 23-30 7380199-5 1980 The rate of gastrin release stimulated by carbachol (1 X10(-5) M) was 0.79 ng-hr-1-mg-1 which was significantly greater than the control value of 0.37 ng-hr-1 (P less than 0.001). Carbachol 42-51 gastrin Rattus norvegicus 12-19 7380199-7 1980 However, carbachol-stimulated gastrin release was inhibited progressively by inclusion of increasing concentrations of atropine in the culture media. Carbachol 9-18 gastrin Rattus norvegicus 30-37 7380199-8 1980 The results of these studies indicated that the acetylcholine analogue, carbachol, is capable of directly stimulating the antral gastrin cell to significantly increase the rate of synthesis and release of gastrin. Carbachol 72-81 gastrin Rattus norvegicus 129-136 7380199-8 1980 The results of these studies indicated that the acetylcholine analogue, carbachol, is capable of directly stimulating the antral gastrin cell to significantly increase the rate of synthesis and release of gastrin. Carbachol 72-81 gastrin Rattus norvegicus 205-212 7380199-9 1980 Whereas atropine, under these in vitro conditions, does not alter basal gastrin release, the muscarinic antagonist does competively inhibit carbachol-stimulated gastrin secretion. Carbachol 140-149 gastrin Rattus norvegicus 161-168 6965318-0 1980 Antibody to Thy-1 antigen injected into rat hypothalamus selectively inhibits carbamyl choline induced drinking. Carbachol 78-94 Thy-1 cell surface antigen Rattus norvegicus 12-25 6246192-4 1980 Voltage- jump relaxations follow an exponential time-course; the rate constants are about twice as large as those seen with 50 muM carbachol and have the same voltage and temperature sensitivity. Carbachol 131-140 latexin Homo sapiens 127-130 44657-1 1979 The perfused stomach in the anaesthetized rat was used to investigate the action of somatostatin on the gastric acid secretion stimulated by gastrin, histamine and carbamylcholine. Carbachol 164-179 somatostatin Rattus norvegicus 84-96 41059-9 1979 Betsamide antagonized the contractile effects of carbachol and 5-hydroxytryptamine on the rat vas deferens, but not the beta-responses of the guinea-pig trachea to adrenaline and isoproenaline. Carbachol 49-58 arginine vasopressin Rattus norvegicus 94-97 464093-9 1979 Carbachol may increase Ca2+ influx as well as utilize sequestered Ca2+. Carbachol 0-9 carbonic anhydrase 2 Homo sapiens 23-26 6153798-1 1980 Substance P inhibits carbamylcholine-induced 22Na+ uptake in the clonal cell line PC12. Carbachol 21-36 tachykinin precursor 1 Homo sapiens 0-11 37961-1 1979 1 Isolated lung parenchymal strips of the dog contracted in response to histamine > carbachol > prostaglandin F(2alpha) (PGF(2alpha)) > bradykinin (Bk) > 5-hydroxytryptamine (5-HT). Carbachol 87-96 kininogen 1 Canis lupus familiaris 145-155 37961-1 1979 1 Isolated lung parenchymal strips of the dog contracted in response to histamine > carbachol > prostaglandin F(2alpha) (PGF(2alpha)) > bradykinin (Bk) > 5-hydroxytryptamine (5-HT). Carbachol 87-96 kininogen 1 Canis lupus familiaris 157-159 464093-9 1979 Carbachol may increase Ca2+ influx as well as utilize sequestered Ca2+. Carbachol 0-9 carbonic anhydrase 2 Homo sapiens 66-69 217365-4 1978 The assay was used to study the time course of the cyclic GMP changes that occur after stimulation of neuroblastoma cells with carbamoylcholine and the dependence of the cyclic GMP changes on the carbamoylcholine concentration. Carbachol 127-143 5'-nucleotidase, cytosolic II Mus musculus 58-61 446418-3 1979 In stress-adapted normal mice, injection of carbamyl choline (CCh) reduced the BRI levels. Carbachol 44-60 integral membrane protein 2B Mus musculus 79-82 446418-3 1979 In stress-adapted normal mice, injection of carbamyl choline (CCh) reduced the BRI levels. Carbachol 62-65 integral membrane protein 2B Mus musculus 79-82 225471-10 1979 It is suggested that superficial AII-excited neurones have a cholinergic excitatory synapse with the deeper carbachol-excited neurones. Carbachol 108-117 angiotensinogen Rattus norvegicus 33-36 225471-18 1979 It is suggested that AII units driven antidromically are actually carbachol-sensitive neurones driven by the more superficial AII-sensitive cells. Carbachol 66-75 angiotensinogen Rattus norvegicus 21-24 469778-7 1979 20 microgram Carbachol elicited a highly significant rise in plasma growth hormone, suggesting a cholinergic component in the neural control of growth hormone in sheep. Carbachol 13-22 somatotropin Ovis aries 68-82 469778-7 1979 20 microgram Carbachol elicited a highly significant rise in plasma growth hormone, suggesting a cholinergic component in the neural control of growth hormone in sheep. Carbachol 13-22 somatotropin Ovis aries 144-158 222025-3 1979 Exogenous cyclic 3",5"-AMP (cAMP) and substances known to increase the intracellular concentration of this nucleotide (isoproterenol, theophylline, noradrenaline, lactate) were shown to inhibit the transport of fluorescein (a weak organic acid) into the rat renal proximal tubules at 20 degrees C. Carbacholine decreasing intracellular cAMP concentration stimulated the transport. Carbachol 298-310 cathelicidin antimicrobial peptide Rattus norvegicus 10-26 216273-6 1978 Carbachol increased modestly, but significantly, the levels of cyclic GMP in isolated toad bladder epithelial cells. Carbachol 0-9 5'-nucleotidase, cytosolic II Homo sapiens 70-73 31192-7 1978 N-Methylhydroxylamine also blocked the increases of cyclic GMP levels by carbachol, prostaglandin E1 and N-methyl-N"-nitro-N-nitrosoguanidine in neuroblastoma N1E 115 cells. Carbachol 73-82 5'-nucleotidase, cytosolic II Mus musculus 59-62 217365-4 1978 The assay was used to study the time course of the cyclic GMP changes that occur after stimulation of neuroblastoma cells with carbamoylcholine and the dependence of the cyclic GMP changes on the carbamoylcholine concentration. Carbachol 196-212 5'-nucleotidase, cytosolic II Mus musculus 177-180 401096-8 1978 VIP induced a dose-dependent relaxation upon contraction by carbamylcholine. Carbachol 60-75 vasoactive intestinal peptide Sus scrofa 0-3 210883-5 1978 Finally, position 8 aliphatic substituted analogues of AII were competitive antagonists of AII-induced drinking, and also inhibited drinking induced by renin, SRS and AI injected through the same intracranial cannula, but they did not inhibit carbachol-induced drinking. Carbachol 243-252 angiotensinogen Rattus norvegicus 55-58 216539-2 1978 Carbamylcholine inhibited somewhat the vasopressin depletion in the neurohypophysis, but not earlier than under severe dehydration (8th and 12th day). Carbachol 0-15 arginine vasopressin Rattus norvegicus 39-50 738215-0 1978 Vasopressin release from incubated in situ posterior pituitary lobe after intraventricular injection of carbachol or atropine. Carbachol 104-113 arginine vasopressin Rattus norvegicus 0-11 738215-3 1978 The increase in the release of vasopressin following intracarotid infusions of hypertonic solution augmented by intraventricular injection of carbachol was found. Carbachol 142-151 arginine vasopressin Rattus norvegicus 31-42 738215-5 1978 The effects of carbachol and atropine indicate that mediation at synapses in the nucleus supraopticus involved in the release of vasopressin induced by osmotic stimulation is cholinergic. Carbachol 15-24 arginine vasopressin Rattus norvegicus 129-140 202611-8 1978 This defect in beige mice could be corrected by chronic administration of carbamyl choline (t((1/2)) = 3.5 h), a cholinergic agonist which raises intracellular cyclic GMP levels. Carbachol 74-90 5'-nucleotidase, cytosolic II Mus musculus 167-170 198531-8 1977 The responses to CCK-OP or carbamylcholine were potentiated by secretin, VIP or dibutyryl cyclic AMP. Carbachol 27-42 vasoactive intestinal peptide Homo sapiens 73-76 198531-10 1977 The responses to secretin or VIP were potentiated by CCK-OP, carbamylcholine, or dibutyryl cyclic GMP. Carbachol 61-76 vasoactive intestinal peptide Homo sapiens 29-32 19825-7 1977 Cyclic GMP levels were increased by carbachol, acetylcholine, and the phosphodiesterase inhibitor, aminophylline, but not by DSCG, or beta-adrenergic agonists. Carbachol 36-45 5'-nucleotidase, cytosolic II Mus musculus 7-10 838187-3 1977 Somatostatin inhibited gastric secretion after all three stimuli, with decreasing potency against carbachol, pentagastrin and histamine. Carbachol 98-107 somatostatin Canis lupus familiaris 0-12 868526-0 1977 Content of vasopressin in the neurohypophysis of long-term dehydrated rats as influenced by carbachol treatment. Carbachol 92-101 arginine vasopressin Rattus norvegicus 11-22 6773423-2 1980 Carbachol caused a small increase in base-line water flow and inhibited, partially, vasopressin- (AVP) or cyclic AMP-stimulated water flow. Carbachol 0-9 arginine vasopressin Homo sapiens 84-95 929114-0 1977 The influence of graded distension and carbachol on the motor response to cholecystokinin in isolated guinea-pig antrum and fundus. Carbachol 39-48 cholecystokinin Cavia porcellus 74-89 929114-3 1977 The responses to cholecystokinin were additive with low doses (10(-7)M) of carbachol, but diminished or abolished by prestimulation with higher doses. Carbachol 75-84 cholecystokinin Cavia porcellus 17-32 868526-1 1977 Content of vasopressin in the neurohypophysis of long-term dehydrated rats as influenced by carbachol treatment. Carbachol 92-101 arginine vasopressin Rattus norvegicus 11-22 868526-5 1977 Under extreme dehydration (8 and 12 days) the vasopressin depletion in the neurohypophysis was significantly inhibited in the carbachol-treated animals. Carbachol 126-135 arginine vasopressin Rattus norvegicus 46-57 181380-4 1976 With CCK-OP an effect on both functions could be detected at 10(-10) M and maximal stimulation occurred at 3 X 10(-8) M. With carbamylcholine an effect on both functions could be detected at 10(-5) M and maximal stimulation occurred at 3 X 10(-3) M. Atropine inhibited stimulation of both cyclic GMP and calcium outflux by carbamylcholine but not by CCK-OP. Carbachol 126-141 cholecystokinin Cavia porcellus 5-8 197234-7 1977 Carbachol (100 micrometer) caused a twofold increase in cyclic GMP without affecting cyclic AMP levels. Carbachol 0-9 5'-nucleotidase, cytosolic II Homo sapiens 63-66 949726-8 1976 Stimulation of the submandibular gland with carbachol (2 mg/kg) led to a massive release of the content of the secretory granules, including NGF, into the salivary duct. Carbachol 44-53 nerve growth factor Mus musculus 141-144 962435-1 1976 Desensitization of the isolated rat vas deferens was demonstrated by using noradrenaline, 5-hydroxytryptamine, carbachol, acetylcholine or barium chloride as a test concentration after a large concentration (concentration producing a contraction that was 80% of the maximum) of the same agonist. Carbachol 111-120 arginine vasopressin Rattus norvegicus 36-39 181380-4 1976 With CCK-OP an effect on both functions could be detected at 10(-10) M and maximal stimulation occurred at 3 X 10(-8) M. With carbamylcholine an effect on both functions could be detected at 10(-5) M and maximal stimulation occurred at 3 X 10(-3) M. Atropine inhibited stimulation of both cyclic GMP and calcium outflux by carbamylcholine but not by CCK-OP. Carbachol 126-141 cholecystokinin Cavia porcellus 350-353 6897-0 1976 Induction of tyrosine 3-monooxygenase elicited by carbamylcholine in intact and denervated adrenal medulla: role of protein kinase activation and translocation. Carbachol 50-65 tyrosine hydroxylase Homo sapiens 13-37 1278094-5 1976 The cholinergic agonists arecoline, nicotine, and carbachol significantly inhibited the afternoon surge of prolactin. Carbachol 50-59 prolactin Rattus norvegicus 107-116 4115-8 1976 Conversely, carbamylcholine rapidly increased lymphocyte cyclic GMP but was not mitogenic. Carbachol 12-27 5'-nucleotidase, cytosolic II Mus musculus 64-67 1066992-8 1976 Rat diaphragms and eel electroplax incubated with antisera to Torpedo and eel AChR showed a 50%-60% reduction in carbamylcholine depolarization. Carbachol 113-128 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 78-82 1262469-13 1976 Animals treated for 3 wk and longer with carbamylcholine or carbamyl beta-methylcholline show normal granule morphology and a normal degree of concanavalin A cap formation in peripheral blood PMN leukocytes. Carbachol 41-56 tubulin-specific chaperone E Mus musculus 192-195 813769-9 1976 The carbachol effect is much less dependent on calcium, as it occurs even in a Ca2+ -free medium containing 10(-4) M ethyleneglycol-bis(beta-aminoethylether)-N,N-tetraacetic acid. Carbachol 4-13 carbonic anhydrase 2 Oryctolagus cuniculus 79-82 813769-11 1976 Carbachol causes a marked increase in the 45Ca2+ efflux from pre-loaded pancreas fragments in both a normal Krebs-Ringer bicarbonate medium and this Ca2+ -free medium. Carbachol 0-9 carbonic anhydrase 2 Oryctolagus cuniculus 44-47 813769-16 1976 It is concluded that both substances stimulate pancreatic enzyme secretion by increasing the cytoplasmic calcium concentration, through an increase in the calcium permeability of the plasma membrane in the case of the ionophore, and through a release of Ca2+ from intracellular stores in the case of carbachol. Carbachol 300-309 carbonic anhydrase 2 Oryctolagus cuniculus 254-257 173397-10 1976 The Ca2+ antagonist D600 blocks the stimulatory effects of both carbamylcholine and pancreozymin only partially. Carbachol 64-79 carbonic anhydrase 2 Rattus norvegicus 4-7 7752-7 1976 A 4 h pulse of 10(-4) M carbamylcholine produced optimal induction of DBH and TH 24 h and 48 h later respectively. Carbachol 24-39 tyrosine hydroxylase Rattus norvegicus 78-80 7752-5 1976 Carbamylcholine, acetylcholine and nicotine at concentrations of 10(-4) M elicited a selective induction of TH and DBH both in intact and decentralized ganglia via nicotinic receptor stimulation. Carbachol 0-15 tyrosine hydroxylase Rattus norvegicus 108-110 2926-4 1976 Hypertonic saline and carbachol suppressed renin release. Carbachol 22-31 renin Rattus norvegicus 43-48 7752-5 1976 Carbamylcholine, acetylcholine and nicotine at concentrations of 10(-4) M elicited a selective induction of TH and DBH both in intact and decentralized ganglia via nicotinic receptor stimulation. Carbachol 0-15 dopamine beta-hydroxylase Rattus norvegicus 115-118 7752-7 1976 A 4 h pulse of 10(-4) M carbamylcholine produced optimal induction of DBH and TH 24 h and 48 h later respectively. Carbachol 24-39 dopamine beta-hydroxylase Rattus norvegicus 70-73 7752-8 1976 Longer exposure to carbamylcholine resulted in a significantly smaller rise in TH activity. Carbachol 19-34 tyrosine hydroxylase Rattus norvegicus 79-81 1193326-2 1975 CCH induced a sharp decrease in D50 (dose of secretin which elicits half the calculated maximal response) but no increase in maximal response to secretin. Carbachol 0-3 SCT Canis lupus familiaris 45-53 1265437-5 1976 It is concluded, that the carbachol-induced rise in the basal sphincter pressure is not dependent on an increase in serum gastrin, but probably attributable to either direct cholinergic stimulation of the receptors or a cholinergically enhanced sensitivity of the receptors for gastrin. Carbachol 26-35 gastrin Homo sapiens 278-285 1167546-6 1975 The carbamylcholine-induced Na+ transport activity of the receptor is inhibited 50% by 4 muM D-tubocurarine, 100 muM atropine, or 1.6 nM diiodo-alpha-bungarotoxin but is not affected by tetrodotoxin. Carbachol 4-19 latexin Homo sapiens 89-92 1167546-6 1975 The carbamylcholine-induced Na+ transport activity of the receptor is inhibited 50% by 4 muM D-tubocurarine, 100 muM atropine, or 1.6 nM diiodo-alpha-bungarotoxin but is not affected by tetrodotoxin. Carbachol 4-19 latexin Homo sapiens 113-116 1193326-3 1975 Experiments performed under different haemodynamic conditions show that this potentiating effect (synergism) is partly due to vasomotor modifications and chiefly to the action of CCH on the receptor of secretin. Carbachol 179-182 SCT Canis lupus familiaris 202-210 235784-4 1975 The results indicate an effect of carbachol on acid, pepsin, and IF secretion, independent of changes in plasma gastrin concentration. Carbachol 34-43 cobalamin binding intrinsic factor Homo sapiens 65-67 4365701-1 1973 Low concentrations of insulin (120 muunits/ml) and of carbamylcholine (1 muM) increase cyclic GMP content in isolated fat cells by 350%. Carbachol 54-69 5'-nucleotidase, cytosolic II Homo sapiens 94-97 235784-2 1975 The volume of gastric secretion and the concentration and output of acid, pepsin, and IF increased significantly during infusion of carbachol alone, whereas the plasma gastrin concentration remained unchanged. Carbachol 132-141 cobalamin binding intrinsic factor Homo sapiens 86-88 4437728-0 1974 Reduced behavioural effects of intrahippocampally administered carbachol in rats with low cholinesterase activity. Carbachol 63-72 butyrylcholinesterase Rattus norvegicus 90-104 4357615-7 1974 Incubation of cells with exogenous cAMP or with agents that increase endogenous cAMP levels (prostaglandin E1, histamine, isoproterenol, and cholera enterotoxin) reduced extrusion of lysosomal enzymes; in contrast, exogenous cGMP and carbamylcholine chloride (which increases endogenous cGMP levels), increased beta-glucuronidase release. Carbachol 234-258 cathelicidin antimicrobial peptide Homo sapiens 35-39 4357615-7 1974 Incubation of cells with exogenous cAMP or with agents that increase endogenous cAMP levels (prostaglandin E1, histamine, isoproterenol, and cholera enterotoxin) reduced extrusion of lysosomal enzymes; in contrast, exogenous cGMP and carbamylcholine chloride (which increases endogenous cGMP levels), increased beta-glucuronidase release. Carbachol 234-258 cathelicidin antimicrobial peptide Homo sapiens 80-84 4365701-2 1973 The maximal amount of cyclic GMP, achieved within 2 min after addition of insulin or carbamylcholine, falls rapidly for insulin and much more slowly for carbamylcholine. Carbachol 85-100 5'-nucleotidase, cytosolic II Homo sapiens 29-32 4365701-2 1973 The maximal amount of cyclic GMP, achieved within 2 min after addition of insulin or carbamylcholine, falls rapidly for insulin and much more slowly for carbamylcholine. Carbachol 85-100 insulin Homo sapiens 120-127 4365701-2 1973 The maximal amount of cyclic GMP, achieved within 2 min after addition of insulin or carbamylcholine, falls rapidly for insulin and much more slowly for carbamylcholine. Carbachol 153-168 5'-nucleotidase, cytosolic II Homo sapiens 29-32 4365701-2 1973 The maximal amount of cyclic GMP, achieved within 2 min after addition of insulin or carbamylcholine, falls rapidly for insulin and much more slowly for carbamylcholine. Carbachol 153-168 insulin Homo sapiens 74-81 4365701-5 1973 Insulin and carbamylcholine increase the concentration of cyclic GMP in rat-liver slices by 400%; the effects of both agents occur rapidly and are relatively transient. Carbachol 12-27 5'-nucleotidase, cytosolic II Homo sapiens 65-68 4365701-7 1973 Carbamylcholine, however, causes a substantial increase in the cyclic GMP content of these lymphocytes. Carbachol 0-15 5'-nucleotidase, cytosolic II Homo sapiens 70-73 4764536-0 1973 The relationship between inhibition of acetylcholinesterase and sensitivity of the rat ileum to carbachol. Carbachol 96-105 acetylcholinesterase Rattus norvegicus 39-59 5569117-0 1971 Effect of carbachol on phosphoenolpyruvate carboxykinase and glucokinase in rat liver. Carbachol 10-19 glucokinase Rattus norvegicus 61-72 24173288-2 1971 The effect of carbamylcholine (Carb) on the release of(22)Na(+) by excitable microsacs is reversible: Carb does not promote an irreversible lysis of the microsacs. Carbachol 14-29 syntaxin 8 Homo sapiens 31-35 4764290-0 1973 Response of the rat ileum, uterus and vas deferens to carbachol and acetylcholine following repeated daily administration of a cholinesterase inhibitor. Carbachol 54-63 butyrylcholinesterase Rattus norvegicus 127-141 5104566-0 1971 Release of oxytocin and vasopressin in lactating rats after injection of carbachol into the third ventricle. Carbachol 73-82 arginine vasopressin Rattus norvegicus 24-35 5272331-1 1970 p-Nitrobenzene diazonium fluoroborate (NDF) is a potent inhibitor of the carbamylcholine-induced depolarization of the electroplax and of acetylcholinesterase. Carbachol 73-88 neuregulin 1 Homo sapiens 39-42 5272331-1 1970 p-Nitrobenzene diazonium fluoroborate (NDF) is a potent inhibitor of the carbamylcholine-induced depolarization of the electroplax and of acetylcholinesterase. Carbachol 73-88 acetylcholinesterase (Cartwright blood group) Homo sapiens 138-158 5767890-4 1969 In addition, these units are excited by intracarotid injections of carbachol, acetylcholine and NaCl (5%) which are less effective stimuli for vasopressin release.3. Carbachol 67-76 arginine vasopressin Rattus norvegicus 143-154 13824696-0 1960 Inhibition of cholinesterase with methylfluorophosphorylcholine and carbocholine: spontaneous return of activity. Carbachol 68-80 butyrylcholinesterase Homo sapiens 14-28 33915166-9 2021 In agreement with reduced CCKBC number in TLE, electrical recordings revealed a significant reduction in amplitude and frequency of IPSCs in CA1 PCs evoked by carbachol (commonly used to excite CCK+ interneurons) in ventral CA1 regions from epileptic mice versus sham controls. Carbachol 159-168 carbonic anhydrase 1 Mus musculus 141-144 33795544-6 2021 In carbachol-contracted gastric and ileal strips, contractile changes were recorded by adding NES- 1 (0.3 nmol/L), GLP-1, CCK-1, and gastrin/CCK-2 antagonists. Carbachol 3-12 gastrin Rattus norvegicus 133-140 4556090-0 1972 Influence of acetyl- -methylcholine, carbamoylcholine, and bis-pyridinium compounds on the activity of acetylcholinesterase. Carbachol 37-53 acetylcholinesterase (Cartwright blood group) Homo sapiens 103-123 4625268-13 1972 The time-course of post-carbachol hyperpolarization accorded with a Na(+) extrusion process whose rate was directly proportional to [Na(+)](i) with a rate constant of 0.38+/-0.02 min(-1) at 23-27 degrees C.9. Carbachol 24-33 complement C9 Rattus norvegicus 204-207 33915166-9 2021 In agreement with reduced CCKBC number in TLE, electrical recordings revealed a significant reduction in amplitude and frequency of IPSCs in CA1 PCs evoked by carbachol (commonly used to excite CCK+ interneurons) in ventral CA1 regions from epileptic mice versus sham controls. Carbachol 159-168 carbonic anhydrase 1 Mus musculus 224-227 33897375-5 2021 Application of the cholinomimetic drug carbachol leads to a decrease in DAT activity in primary cultures while the M1/M5-specific antagonist, pirenzepine, blocks these effects. Carbachol 39-48 solute carrier family 6 member 3 Homo sapiens 72-75 33865856-4 2021 We report here that monomeric wild type (WT) mouse SK1 (GFP-mSK1) translocates to the PM of MCF-7L cells stimulated with carbachol or phorbol myristate acetate (PMA), whereas the dimer translocates to the PM in response to sphingosine 1-phosphate (S1P); thus, the equilibrium between monomer and dimer is sensitive to cellular stimulus. Carbachol 121-130 sphingosine kinase 1 Mus musculus 51-54 33865856-4 2021 We report here that monomeric wild type (WT) mouse SK1 (GFP-mSK1) translocates to the PM of MCF-7L cells stimulated with carbachol or phorbol myristate acetate (PMA), whereas the dimer translocates to the PM in response to sphingosine 1-phosphate (S1P); thus, the equilibrium between monomer and dimer is sensitive to cellular stimulus. Carbachol 121-130 skin antigen 1 Mus musculus 60-64 33865856-5 2021 In addition, carbachol and PMA induced translocation of monomeric GFP-mSK1 to lamellipodia, while S1P induced translocation of dimeric GFP-mSK1 to filopodia, suggesting that SK1 regulates different cell biological processes dependent on dimerization. Carbachol 13-22 skin antigen 1 Mus musculus 70-74 33865856-5 2021 In addition, carbachol and PMA induced translocation of monomeric GFP-mSK1 to lamellipodia, while S1P induced translocation of dimeric GFP-mSK1 to filopodia, suggesting that SK1 regulates different cell biological processes dependent on dimerization. Carbachol 13-22 sphingosine kinase 1 Homo sapiens 71-74 33897375-6 2021 The M3 antagonist, DAU 5884, does not affect, but a positive modulator of M5, VU 0238429, enhances the loss of DAT function in response to carbachol and acetylcholine. Carbachol 139-148 solute carrier family 6 member 3 Homo sapiens 111-114 33897375-8 2021 Bisindolylmaleimide, a PKC inhibitor, blocks the effects of carbachol stimulation on dopamine uptake, supporting a role for PKC in muscarinic receptor-mediated DAT internalization. Carbachol 60-69 solute carrier family 6 member 3 Homo sapiens 160-163 33897375-10 2021 A Gq-specific inhibitor peptide also blocks the effects of carbachol on DAT in primary cultures, confirming Gq as the G-protein that couples M1/M5 receptors to PKC activation in these cells. Carbachol 59-68 solute carrier family 6 member 3 Homo sapiens 72-75 33561807-7 2021 RESULTS: Intraperitoneal injection, but not localized intraglandular administration, of AR-C118925 significantly enhanced carbachol-induced salivation and reduced lymphocytic foci and immune cell markers in SMGs of 5 month old NOD.H-2h4 DKO mice, compared to vehicle-injected control mice. Carbachol 122-131 ferredoxin reductase Mus musculus 88-90 33837176-6 2021 Performing extracellular recordings in brain slices from p75NTR knockout mice (p75-/-) in presence of the muscarinic agonist carbachol, we find that gamma oscillation power and rhythmicity are increased compared to wild-type (WT) mice. Carbachol 125-134 nerve growth factor receptor (TNFR superfamily, member 16) Mus musculus 57-63 33562815-5 2021 Live-cell imaging of cultured lacrimal gland acinar cells (LGAC) transduced with adenovirus encoding wild-type (WT) mCFP-Rab27a revealed carbachol-stimulated fusion and depletion of mCFP-Rab27a-enriched vesicles. Carbachol 137-146 complement factor properdin Mus musculus 116-120 33739386-2 2021 We discovered that the application of a cholinergic agonist, carbachol (Cch), which triggers oscillatory activity in the gamma range, induces the activity of matrix metalloproteinase 9 (MMP-9)-an enzyme necessary for the maintenance of synaptic plasticity. Carbachol 61-70 matrix metallopeptidase 9 Homo sapiens 158-184 33739386-2 2021 We discovered that the application of a cholinergic agonist, carbachol (Cch), which triggers oscillatory activity in the gamma range, induces the activity of matrix metalloproteinase 9 (MMP-9)-an enzyme necessary for the maintenance of synaptic plasticity. Carbachol 61-70 matrix metallopeptidase 9 Homo sapiens 186-191 33739386-2 2021 We discovered that the application of a cholinergic agonist, carbachol (Cch), which triggers oscillatory activity in the gamma range, induces the activity of matrix metalloproteinase 9 (MMP-9)-an enzyme necessary for the maintenance of synaptic plasticity. Carbachol 72-75 matrix metallopeptidase 9 Homo sapiens 158-184 33739386-2 2021 We discovered that the application of a cholinergic agonist, carbachol (Cch), which triggers oscillatory activity in the gamma range, induces the activity of matrix metalloproteinase 9 (MMP-9)-an enzyme necessary for the maintenance of synaptic plasticity. Carbachol 72-75 matrix metallopeptidase 9 Homo sapiens 186-191 33739386-3 2021 Using electrophysiological recordings in hippocampal organotypic slices, we show that Cch potentiates the frequency of miniature inhibitory and excitatory postsynaptic currents (mIPSCs and mEPSCs, respectively) in CA1 neurons and this effect is MMP-9 dependent. Carbachol 86-89 carbonic anhydrase 1 Homo sapiens 214-217 33739386-3 2021 Using electrophysiological recordings in hippocampal organotypic slices, we show that Cch potentiates the frequency of miniature inhibitory and excitatory postsynaptic currents (mIPSCs and mEPSCs, respectively) in CA1 neurons and this effect is MMP-9 dependent. Carbachol 86-89 matrix metallopeptidase 9 Homo sapiens 245-250 33562815-5 2021 Live-cell imaging of cultured lacrimal gland acinar cells (LGAC) transduced with adenovirus encoding wild-type (WT) mCFP-Rab27a revealed carbachol-stimulated fusion and depletion of mCFP-Rab27a-enriched vesicles. Carbachol 137-146 RAB27A, member RAS oncogene family Mus musculus 121-127 33562815-5 2021 Live-cell imaging of cultured lacrimal gland acinar cells (LGAC) transduced with adenovirus encoding wild-type (WT) mCFP-Rab27a revealed carbachol-stimulated fusion and depletion of mCFP-Rab27a-enriched vesicles. Carbachol 137-146 RAB27A, member RAS oncogene family Mus musculus 187-193 33528684-6 2021 However, 7,8-DHF enhanced CCh-stimulated PLCgamma1 activation and strip contraction. Carbachol 26-29 phospholipase C, gamma 1 Rattus norvegicus 41-50 33528684-11 2021 ANA-12, a specific TrkB antagonist, not only inhibited TrkB activation by 7,8-DHF but also suppressed 7,8-DHF-enhanced cholinergic contraction, 7,8-DHF/CCh-mediated activation of PLCgamma1/Akt, and M3 overexpression in colonic strips. Carbachol 152-155 neurotrophic receptor tyrosine kinase 2 Rattus norvegicus 19-23 33562815-6 2021 LGAC transduced with dominant-negative (DN) mCFP-Rab27a exhibited significantly reduced carbachol-stimulated CTSS secretion by 0.5-fold and beta-hexosaminidase by 0.3-fold, relative to stimulated LGAC transduced with WT mCFP-Rab27a. Carbachol 88-97 complement factor properdin Mus musculus 44-48 33562815-6 2021 LGAC transduced with dominant-negative (DN) mCFP-Rab27a exhibited significantly reduced carbachol-stimulated CTSS secretion by 0.5-fold and beta-hexosaminidase by 0.3-fold, relative to stimulated LGAC transduced with WT mCFP-Rab27a. Carbachol 88-97 RAB27A, member RAS oncogene family Mus musculus 49-55 33562815-6 2021 LGAC transduced with dominant-negative (DN) mCFP-Rab27a exhibited significantly reduced carbachol-stimulated CTSS secretion by 0.5-fold and beta-hexosaminidase by 0.3-fold, relative to stimulated LGAC transduced with WT mCFP-Rab27a. Carbachol 88-97 cathepsin S Mus musculus 109-113 33562815-6 2021 LGAC transduced with dominant-negative (DN) mCFP-Rab27a exhibited significantly reduced carbachol-stimulated CTSS secretion by 0.5-fold and beta-hexosaminidase by 0.3-fold, relative to stimulated LGAC transduced with WT mCFP-Rab27a. Carbachol 88-97 complement factor properdin Mus musculus 220-224 33562815-6 2021 LGAC transduced with dominant-negative (DN) mCFP-Rab27a exhibited significantly reduced carbachol-stimulated CTSS secretion by 0.5-fold and beta-hexosaminidase by 0.3-fold, relative to stimulated LGAC transduced with WT mCFP-Rab27a. Carbachol 88-97 RAB27A, member RAS oncogene family Mus musculus 225-231 33528684-7 2021 The PLCgamma1 antagonist U73122 suppressed both CCh-stimulated and 7,8-DHF-enhanced/CCh-stimulated contraction. Carbachol 48-51 phospholipase C, gamma 1 Rattus norvegicus 4-13 33528684-7 2021 The PLCgamma1 antagonist U73122 suppressed both CCh-stimulated and 7,8-DHF-enhanced/CCh-stimulated contraction. Carbachol 84-87 phospholipase C, gamma 1 Rattus norvegicus 4-13 33528684-10 2021 Since 7,8-DHF increased Akt phosphorylation, and LY294002 (an antagonist of PI3K upstream of Akt) dramatically inhibited both 7,8-DHF-augmented M3 expression and 7,8-DHF-enhanced/CCh-stimulated contractions, we assumed that 7,8-DHF/TrkB/Akt was associated with the modulation of M3 expression in the colonic strips. Carbachol 179-182 AKT serine/threonine kinase 1 Rattus norvegicus 93-96 33528684-10 2021 Since 7,8-DHF increased Akt phosphorylation, and LY294002 (an antagonist of PI3K upstream of Akt) dramatically inhibited both 7,8-DHF-augmented M3 expression and 7,8-DHF-enhanced/CCh-stimulated contractions, we assumed that 7,8-DHF/TrkB/Akt was associated with the modulation of M3 expression in the colonic strips. Carbachol 179-182 AKT serine/threonine kinase 1 Rattus norvegicus 93-96 33537462-4 2021 Here, we show that TMEM16A protein abundance correlates with Cl- secretion in different regions of native intestine activated by the Ca2+-elevating muscarinic agonist carbachol (CCH). Carbachol 167-176 anoctamin 1 Homo sapiens 19-26 33537462-4 2021 Here, we show that TMEM16A protein abundance correlates with Cl- secretion in different regions of native intestine activated by the Ca2+-elevating muscarinic agonist carbachol (CCH). Carbachol 178-181 anoctamin 1 Homo sapiens 19-26 33537462-5 2021 Basal, as well as both cAMP- and CCH-stimulated Isc, was largely reduced in Ano1 +- mouse intestine. Carbachol 33-36 anoctamin 1, calcium activated chloride channel Mus musculus 76-80 33537462-8 2021 Cellular depletion of NHERF1 in human colonic T84 cells caused a significant reduction of both cAMP- and CCH-stimulated Isc. Carbachol 105-108 SLC9A3 regulator 1 Homo sapiens 22-28 31910704-4 2020 Cumulative concentration response curves were constructed to OT and then the strips were incubated with atosiban (OT antagonist) and a second concentration response curve to OT were constructed.Results: Carbachol, contracted all human strips for the functionality test whereas OT in any concentrations did not produce significant contraction on all human strips. Carbachol 203-212 oxytocin/neurophysin I prepropeptide Homo sapiens 61-63 33390980-5 2020 Ketamine (100 microM) strongly inhibited both carbachol- and GTPgammaS-induced mICAT. Carbachol 46-55 catenin beta interacting protein 1 Mus musculus 79-84 33563876-3 2021 The Loxl1-/- rats showed increased looseness and redundancy of the skin, the decreased intercontraction interval and voided volume in cystometry, the lower leak-point pressure, thinner elastic fibers of the mesentery, bladder, urethra and vagina, and smaller contractile response of detrusor strips to carbachol when compared to the WT rats. Carbachol 302-311 lysyl oxidase-like 1 Rattus norvegicus 4-9 31910704-4 2020 Cumulative concentration response curves were constructed to OT and then the strips were incubated with atosiban (OT antagonist) and a second concentration response curve to OT were constructed.Results: Carbachol, contracted all human strips for the functionality test whereas OT in any concentrations did not produce significant contraction on all human strips. Carbachol 203-212 oxytocin/neurophysin I prepropeptide Homo sapiens 114-116 31910704-4 2020 Cumulative concentration response curves were constructed to OT and then the strips were incubated with atosiban (OT antagonist) and a second concentration response curve to OT were constructed.Results: Carbachol, contracted all human strips for the functionality test whereas OT in any concentrations did not produce significant contraction on all human strips. Carbachol 203-212 oxytocin/neurophysin I prepropeptide Homo sapiens 114-116 31910704-4 2020 Cumulative concentration response curves were constructed to OT and then the strips were incubated with atosiban (OT antagonist) and a second concentration response curve to OT were constructed.Results: Carbachol, contracted all human strips for the functionality test whereas OT in any concentrations did not produce significant contraction on all human strips. Carbachol 203-212 oxytocin/neurophysin I prepropeptide Homo sapiens 114-116 32801121-6 2020 In our established human salivary gland-derived organoid culture system, we successfully induced an organoid swelling revealing the function of saliva secretion by the stimulation of carbachol, a non-selective cholinergic agonist, and forskolin, an activator of cystic fibrosis transmembrane conductance regulator (CFTR). Carbachol 183-192 CF transmembrane conductance regulator Homo sapiens 262-313 33172956-6 2021 In contrast, Gnb5-/- mice exhibited profound bradycardia on treatment with carbachol, while sympathetic modulation of the cardiac stimulation was not altered. Carbachol 75-84 guanine nucleotide binding protein (G protein), beta 5 Mus musculus 13-17 32065420-7 2020 The gene and protein expression of collagen I (COL1), TIMP-1, and TIMP-2 was carbachol (CCH) concentration-dependently enhanced. Carbachol 77-86 TIMP metallopeptidase inhibitor 1 Homo sapiens 54-60 32065420-7 2020 The gene and protein expression of collagen I (COL1), TIMP-1, and TIMP-2 was carbachol (CCH) concentration-dependently enhanced. Carbachol 77-86 TIMP metallopeptidase inhibitor 2 Homo sapiens 66-72 32065420-7 2020 The gene and protein expression of collagen I (COL1), TIMP-1, and TIMP-2 was carbachol (CCH) concentration-dependently enhanced. Carbachol 88-91 TIMP metallopeptidase inhibitor 1 Homo sapiens 54-60 32065420-7 2020 The gene and protein expression of collagen I (COL1), TIMP-1, and TIMP-2 was carbachol (CCH) concentration-dependently enhanced. Carbachol 88-91 TIMP metallopeptidase inhibitor 2 Homo sapiens 66-72 32065420-9 2020 The CCH-induced protein expression of COL1, TIMP-1, and TIMP-2, however, was obviously reduced by the pretreatment of muscarinic receptor antagonists, atropine, and M3 -preferring antagonist (1,1-dimethyl-4-diphenyl-acetoxypiperidinium iodide [4-DAMP]). Carbachol 4-7 TIMP metallopeptidase inhibitor 1 Homo sapiens 44-50 32065420-9 2020 The CCH-induced protein expression of COL1, TIMP-1, and TIMP-2, however, was obviously reduced by the pretreatment of muscarinic receptor antagonists, atropine, and M3 -preferring antagonist (1,1-dimethyl-4-diphenyl-acetoxypiperidinium iodide [4-DAMP]). Carbachol 4-7 TIMP metallopeptidase inhibitor 2 Homo sapiens 56-62 32065420-10 2020 Furthermore, ERK1/2 was activated by 100 microM CCH when compared with the control group and the pretreatment of ERK1/2 inhibitor significantly suppressed the synthesis of COL1 induced by 100 microM CCH. Carbachol 48-51 mitogen-activated protein kinase 3 Homo sapiens 13-19 32065420-10 2020 Furthermore, ERK1/2 was activated by 100 microM CCH when compared with the control group and the pretreatment of ERK1/2 inhibitor significantly suppressed the synthesis of COL1 induced by 100 microM CCH. Carbachol 48-51 mitogen-activated protein kinase 3 Homo sapiens 113-119 32065420-10 2020 Furthermore, ERK1/2 was activated by 100 microM CCH when compared with the control group and the pretreatment of ERK1/2 inhibitor significantly suppressed the synthesis of COL1 induced by 100 microM CCH. Carbachol 199-202 mitogen-activated protein kinase 3 Homo sapiens 13-19 32065420-10 2020 Furthermore, ERK1/2 was activated by 100 microM CCH when compared with the control group and the pretreatment of ERK1/2 inhibitor significantly suppressed the synthesis of COL1 induced by 100 microM CCH. Carbachol 199-202 mitogen-activated protein kinase 3 Homo sapiens 113-119 32065420-11 2020 Besides, CCH-induced phosphorylation of ERK1/2 was remarkably restrained by the pretreatment of 4-DAMP. Carbachol 9-12 mitogen-activated protein kinase 3 Homo sapiens 40-46 31975215-8 2020 Knockdown of ACOX3 in HASMC, using siRNA, significantly repressed HASMC contraction triggered by carbachol. Carbachol 97-106 acyl-CoA oxidase 3, pristanoyl Homo sapiens 13-18 31975215-9 2020 The carbachol-induced HASMC contraction was restored by transfection with plasmids encoding siRNA-resistant wild-type ACOX3, but not by transfection with ACOX3 G222E or by co-transfection with ACOX3 F79 V and ACOX3 G222E, indicating that the two ACOX3 mutants suppress carbachol-induced HASMC contraction. Carbachol 4-13 acyl-CoA oxidase 3, pristanoyl Homo sapiens 118-123 33041794-10 2020 UCL 1684 reduced carbachol-evoked ASM contractions (>90%, IC50 0.43 muM), and calcium mobilization in rodent and human lung ASM cells. Carbachol 17-26 H19 imprinted maternally expressed transcript Homo sapiens 34-37 32394805-5 2020 We show that in MCA of rats with SCI, there is 55% reduction in the maximal vasodilator response to carbachol, which is associated with reduced expression of endothelial marker cluster of differentiation 31 (CD31) and transient receptor potential cation channel 4 (TRPV4) channels. Carbachol 100-109 transient receptor potential cation channel, subfamily V, member 4 Rattus norvegicus 265-270 32911509-5 2020 The addition of carbachol or arecaidine propargyl ester, a non-selective or a selective subtype 2 muscarinic receptor agonist respectively, plus paclitaxel reduces cell viability involving a down-regulation in the expression of ATP "binding cassette" G2 drug transporter and epidermal growth factor receptor. Carbachol 16-25 epidermal growth factor receptor Homo sapiens 275-307 32765779-0 2020 Carbachol protects the intestinal barrier in severe acute pancreatitis by regulating Cdc42/F-actin cytoskeleton. Carbachol 0-9 cell division cycle 42 Rattus norvegicus 85-90 32765779-10 2020 In addition, the expression of Cdc42, F-actin and claudin-2 was significantly higher in the SAP and SAP + carbachol groups compared with the SO and SO + carbachol groups (P<0.05), and the expression of occludin and zonula occludens-1 were significantly higher in the SO and SO + carbachol groups compared with the SAP and SAP + carbachol groups (P<0.05). Carbachol 106-115 cell division cycle 42 Rattus norvegicus 31-36 32765779-10 2020 In addition, the expression of Cdc42, F-actin and claudin-2 was significantly higher in the SAP and SAP + carbachol groups compared with the SO and SO + carbachol groups (P<0.05), and the expression of occludin and zonula occludens-1 were significantly higher in the SO and SO + carbachol groups compared with the SAP and SAP + carbachol groups (P<0.05). Carbachol 106-115 claudin 2 Rattus norvegicus 50-59 32765779-10 2020 In addition, the expression of Cdc42, F-actin and claudin-2 was significantly higher in the SAP and SAP + carbachol groups compared with the SO and SO + carbachol groups (P<0.05), and the expression of occludin and zonula occludens-1 were significantly higher in the SO and SO + carbachol groups compared with the SAP and SAP + carbachol groups (P<0.05). Carbachol 106-115 occludin Rattus norvegicus 202-210 32765779-10 2020 In addition, the expression of Cdc42, F-actin and claudin-2 was significantly higher in the SAP and SAP + carbachol groups compared with the SO and SO + carbachol groups (P<0.05), and the expression of occludin and zonula occludens-1 were significantly higher in the SO and SO + carbachol groups compared with the SAP and SAP + carbachol groups (P<0.05). Carbachol 153-162 cell division cycle 42 Rattus norvegicus 31-36 32765779-10 2020 In addition, the expression of Cdc42, F-actin and claudin-2 was significantly higher in the SAP and SAP + carbachol groups compared with the SO and SO + carbachol groups (P<0.05), and the expression of occludin and zonula occludens-1 were significantly higher in the SO and SO + carbachol groups compared with the SAP and SAP + carbachol groups (P<0.05). Carbachol 153-162 cell division cycle 42 Rattus norvegicus 31-36 32765779-10 2020 In addition, the expression of Cdc42, F-actin and claudin-2 was significantly higher in the SAP and SAP + carbachol groups compared with the SO and SO + carbachol groups (P<0.05), and the expression of occludin and zonula occludens-1 were significantly higher in the SO and SO + carbachol groups compared with the SAP and SAP + carbachol groups (P<0.05). Carbachol 153-162 cell division cycle 42 Rattus norvegicus 31-36 32765779-11 2020 In conclusion, these findings demonstrated that carbachol may protect the intestinal barrier in the SAP rat model without aggravating pancreatic injury via regulation of Cdc42/F-actin expression. Carbachol 48-57 cell division cycle 42 Rattus norvegicus 170-175 32801121-6 2020 In our established human salivary gland-derived organoid culture system, we successfully induced an organoid swelling revealing the function of saliva secretion by the stimulation of carbachol, a non-selective cholinergic agonist, and forskolin, an activator of cystic fibrosis transmembrane conductance regulator (CFTR). Carbachol 183-192 CF transmembrane conductance regulator Homo sapiens 315-319 32606911-10 2020 Discussion: The findings suggest that chemical stimulation of the LH by carbachol possibly activates the orexin projecting neurons and subsequently, the VTA dopaminergic neurons involved in the orofacial pain modulation. Carbachol 72-81 hypocretin neuropeptide precursor Rattus norvegicus 105-111 32224266-11 2020 Furthermore, isolated atrial preparations and SAN myocytes from AngII infused mice had impaired responses to both ISO and CCh. Carbachol 122-125 angiotensinogen (serpin peptidase inhibitor, clade A, member 8) Mus musculus 64-69 32652816-3 2020 EGFr TKIs (such as afatinib, erlotinib, and osimertinib) reversed the acute inhibitory effect of EGF on chloride secretion induced by carbachol (CCh) across T84 human colonic epithelial cells, which correlated with the diarrhea-inducing effect of each agent clinically. Carbachol 134-143 epidermal growth factor receptor Homo sapiens 0-4 32652816-3 2020 EGFr TKIs (such as afatinib, erlotinib, and osimertinib) reversed the acute inhibitory effect of EGF on chloride secretion induced by carbachol (CCh) across T84 human colonic epithelial cells, which correlated with the diarrhea-inducing effect of each agent clinically. Carbachol 134-143 epidermal growth factor Homo sapiens 0-3 32652816-3 2020 EGFr TKIs (such as afatinib, erlotinib, and osimertinib) reversed the acute inhibitory effect of EGF on chloride secretion induced by carbachol (CCh) across T84 human colonic epithelial cells, which correlated with the diarrhea-inducing effect of each agent clinically. Carbachol 145-148 epidermal growth factor receptor Homo sapiens 0-4 32652816-3 2020 EGFr TKIs (such as afatinib, erlotinib, and osimertinib) reversed the acute inhibitory effect of EGF on chloride secretion induced by carbachol (CCh) across T84 human colonic epithelial cells, which correlated with the diarrhea-inducing effect of each agent clinically. Carbachol 145-148 epidermal growth factor Homo sapiens 0-3 32268182-7 2020 It was also found that Hco-LGC-46 assembles with Hco-ACC-1 and produces a receptor that is over 5-fold more sensitive to ACh and responds to the cholinergic agonists methycholine and carbachol. Carbachol 183-192 putative ligand-gated ion channel 46 Caenorhabditis elegans 27-33 32652816-5 2020 Furthermore, afatinib and erlotinib prevented EGF- or CCh-induced EGFr phosphorylation. Carbachol 54-57 epidermal growth factor receptor Homo sapiens 66-70 32652816-6 2020 EGFr TKIs also suppressed phosphorylation of extracellular signal-regulated kinase (Erk)1/2 in response to EGF, whereas they had weaker effects on CCh-induced Erk1/2 phosphorylation. Carbachol 147-150 epidermal growth factor receptor Homo sapiens 0-4 32652816-6 2020 EGFr TKIs also suppressed phosphorylation of extracellular signal-regulated kinase (Erk)1/2 in response to EGF, whereas they had weaker effects on CCh-induced Erk1/2 phosphorylation. Carbachol 147-150 epidermal growth factor Homo sapiens 0-3 32652816-6 2020 EGFr TKIs also suppressed phosphorylation of extracellular signal-regulated kinase (Erk)1/2 in response to EGF, whereas they had weaker effects on CCh-induced Erk1/2 phosphorylation. Carbachol 147-150 mitogen-activated protein kinase 3 Homo sapiens 159-165 32652816-7 2020 In human EDMs, EGF potentiated ion transport induced by CCh, whereas afatinib reversed this effect. Carbachol 56-59 epidermal growth factor Homo sapiens 15-18 32652816-9 2020 CCh-activated Erk1/2 phosphorylation was relatively insensitive to EGFr TKIs and delayed the deleterious effects of EGFr TKIs on barrier function. Carbachol 0-3 mitogen-activated protein kinase 3 Homo sapiens 14-20 32652816-9 2020 CCh-activated Erk1/2 phosphorylation was relatively insensitive to EGFr TKIs and delayed the deleterious effects of EGFr TKIs on barrier function. Carbachol 0-3 epidermal growth factor receptor Homo sapiens 116-120 32562388-11 2020 We observed a significantly smaller CCh response in Ts65Dn versus control euploid mice in the 6- to 10-min-interval postcarbachol injection. Carbachol 36-39 reciprocal translocation, Chr 16, cytogenetic band C3-4; and Chr 17, cytogenetic band A2, Davisson 65 Mus musculus 52-58 32143816-5 2020 In carbachol pre-contracted strips, GLP-2 (20 nM) evoked a tetrodotoxin (TTX)-sensitive relaxation, similar in shape to the TTX-insensitive of 100 nM VIP. Carbachol 3-12 glucagon-like peptide 2 receptor Mus musculus 36-41 32495900-0 2020 Carbachol alleviates myocardial injury in septic rats through PI3K/AKT signaling pathway. Carbachol 0-9 AKT serine/threonine kinase 1 Rattus norvegicus 67-70 32495900-10 2020 CONCLUSIONS: Carbachol can reduce the release of inflammatory factors in myocardial cells, the expression of apoptotic proteins and the apoptosis of myocardial cells, and improve the cardiac function and survival rate of septic rats by inhibiting the PI3K/AKT signaling pathway. Carbachol 13-22 AKT serine/threonine kinase 1 Rattus norvegicus 256-259 32268182-7 2020 It was also found that Hco-LGC-46 assembles with Hco-ACC-1 and produces a receptor that is over 5-fold more sensitive to ACh and responds to the cholinergic agonists methycholine and carbachol. Carbachol 183-192 Acetylcholine-gated chloride channel subunit acc-1 Caenorhabditis elegans 53-58 31867686-8 2020 In male detrusor tissues, STK16-IN-1 inhibited contractions induced by the cholinergic agonists carbachol and metacholine, and contractions induced by U46619. Carbachol 96-105 serine/threonine kinase 16 Homo sapiens 26-31 32350310-6 2020 Whereas SLC10A7 knockout HAP1 cells showed significantly increased calcium influx after thapsigargin, ionomycin and ATP/carbachol treatment, SLC10A7 overexpression reduced this calcium influx. Carbachol 120-129 solute carrier family 10 member 7 Homo sapiens 8-15 32350310-6 2020 Whereas SLC10A7 knockout HAP1 cells showed significantly increased calcium influx after thapsigargin, ionomycin and ATP/carbachol treatment, SLC10A7 overexpression reduced this calcium influx. Carbachol 120-129 huntingtin associated protein 1 Homo sapiens 25-29 31954803-3 2020 Preincubation of M2R-expressing HEK 293T cells with serum IgG fractions from chagasic patients with cardiovascular dysautonomia, followed by the addition of carbachol, resulted in the attenuation of agonist-induced Gi protein activation and arrestin-2 recruitment. Carbachol 157-166 arrestin beta 1 Homo sapiens 241-251 32069351-8 2020 Furthermore, we examined how the intrinsic properties and action-potential firing were altered in CA2 pyramidal neurons treated with 10 microM carbachol. Carbachol 143-152 carbonic anhydrase 2 Homo sapiens 98-101 32135149-8 2020 Results showed that intra-CA1 administration of SCH-23390 or sulpiride could prevent the intra-LH carbachol-induced antinociception. Carbachol 98-107 carbonic anhydrase 1 Rattus norvegicus 26-29 32308799-3 2020 The antagonistic effect of these 1,4-DHP was tested by modulating the impact of carbachol-dependent mobilization of intracellular Ca2+ in SH-SY5Y cells. Carbachol 80-89 dihydropyrimidinase Homo sapiens 37-40 32089769-7 2020 In the presence of L-NAME, a nitric oxide synthase (NOS) inhibitor, the CCh curve remained unchanged in nonsensitized animals but had increased efficacy and potency in sensitized animals, indicating an inhibition of endothelial NOS but ineffective in inhibiting inducible NOS. Carbachol 72-75 nitric oxide synthase, inducible Cavia porcellus 29-50 32063920-15 2020 In addition, Mlx-8 also blocked the accumulation of total [3H]inositol phosphate induced by muscarinic agonist carbachol. Carbachol 111-120 MAX dimerization protein MLX Rattus norvegicus 13-16 31805312-4 2020 RESULTS: Treatment with IL-13 increased the potency of histamine, carbachol and leukotriene D4 as contractile agonists. Carbachol 66-75 interleukin 13 Homo sapiens 24-29 31932898-4 2020 VIP-induced secretion is mediated by cAMP-activated protein kinase A (PKA), independently of increased [Ca2+]i. Synergistic secretion between VIP and the muscarinic agonist, carbachol, was demonstrated but only with submaximal carbachol. Carbachol 174-183 vasoactive intestinal peptide Rattus norvegicus 0-3 31932898-4 2020 VIP-induced secretion is mediated by cAMP-activated protein kinase A (PKA), independently of increased [Ca2+]i. Synergistic secretion between VIP and the muscarinic agonist, carbachol, was demonstrated but only with submaximal carbachol. Carbachol 227-236 vasoactive intestinal peptide Rattus norvegicus 0-3 31932898-4 2020 VIP-induced secretion is mediated by cAMP-activated protein kinase A (PKA), independently of increased [Ca2+]i. Synergistic secretion between VIP and the muscarinic agonist, carbachol, was demonstrated but only with submaximal carbachol. Carbachol 227-236 vasoactive intestinal peptide Rattus norvegicus 142-145 31932898-11 2020 Additional data suggest ryanodine receptors control VIP/PKA-mediated Ca2+ release from a Ca2+ pool also responsive to maximal carbachol. Carbachol 126-135 vasoactive intestinal peptide Rattus norvegicus 52-55 31909533-6 2020 Murine BSM strips overexpressing desmin or vimentin generated less force in response to KCl and carbachol relative to the levels in control murine BSM strips, an effect associated with increased JNK2 phosphorylation and reduced myosin light chain (MLC20 ) phosphorylation. Carbachol 96-105 desmin Mus musculus 33-39 31909533-6 2020 Murine BSM strips overexpressing desmin or vimentin generated less force in response to KCl and carbachol relative to the levels in control murine BSM strips, an effect associated with increased JNK2 phosphorylation and reduced myosin light chain (MLC20 ) phosphorylation. Carbachol 96-105 vimentin Mus musculus 43-51 31910706-14 2020 Erythroid differentiated K562 cells treated with CCh (100 muM). Carbachol 49-52 latexin Homo sapiens 58-61 31260679-8 2019 The results showed that OX1R and OX2R antagonists dose-dependently decreased the antinociceptive effect of carbachol. Carbachol 107-116 hypocretin receptor 1 Rattus norvegicus 24-28 31767755-6 2019 Furthermore, DJ-1 ablation disturbed calcium efflux-induced IP3R3 degradation after carbachol treatment and caused IP3R3 accumulation at the MAM in vitro. Carbachol 84-93 Parkinsonism associated deglycase Homo sapiens 13-17 31767755-6 2019 Furthermore, DJ-1 ablation disturbed calcium efflux-induced IP3R3 degradation after carbachol treatment and caused IP3R3 accumulation at the MAM in vitro. Carbachol 84-93 inositol 1,4,5-trisphosphate receptor type 3 Homo sapiens 60-65 31493399-7 2019 We analyzed interactions between stromal interaction molecule 1 (STIM1) and SARAF in HEK cells stimulated with carbachol using fluorescence resonance energy transfer microscopy and immunoprecipitation. Carbachol 111-120 stromal interaction molecule 1 Mus musculus 33-63 31493399-7 2019 We analyzed interactions between stromal interaction molecule 1 (STIM1) and SARAF in HEK cells stimulated with carbachol using fluorescence resonance energy transfer microscopy and immunoprecipitation. Carbachol 111-120 stromal interaction molecule 1 Mus musculus 65-70 31493399-7 2019 We analyzed interactions between stromal interaction molecule 1 (STIM1) and SARAF in HEK cells stimulated with carbachol using fluorescence resonance energy transfer microscopy and immunoprecipitation. Carbachol 111-120 store-operated calcium entry-associated regulatory factor Mus musculus 76-81 31493399-13 2019 STIM1 interacted stably with SARAF following stimulation of HEK or mouse acinar cells with physiologic levels of carbachol, but only transiently following stimulation with pathologic levels of carbachol, leading to excessive Ca2+ influx. Carbachol 113-122 stromal interaction molecule 1 Mus musculus 0-5 31493399-13 2019 STIM1 interacted stably with SARAF following stimulation of HEK or mouse acinar cells with physiologic levels of carbachol, but only transiently following stimulation with pathologic levels of carbachol, leading to excessive Ca2+ influx. Carbachol 113-122 store-operated calcium entry-associated regulatory factor Mus musculus 29-34 31493399-13 2019 STIM1 interacted stably with SARAF following stimulation of HEK or mouse acinar cells with physiologic levels of carbachol, but only transiently following stimulation with pathologic levels of carbachol, leading to excessive Ca2+ influx. Carbachol 193-202 stromal interaction molecule 1 Mus musculus 0-5 31411061-7 2019 By contrast, carbachol-induced contraction of the ASM derived from Pkd2SM-CKO mice was significantly reduced compared with that in wild-type mice. Carbachol 13-22 H19 imprinted maternally expressed transcript Homo sapiens 50-53 31411061-7 2019 By contrast, carbachol-induced contraction of the ASM derived from Pkd2SM-CKO mice was significantly reduced compared with that in wild-type mice. Carbachol 13-22 polycystin 2, transient receptor potential cation channel Mus musculus 67-73 31411061-8 2019 In addition, relaxation of the carbachol-precontracted ASM by isoprenaline, a beta-adrenergic receptor agonist that acts through the cAMP/adenylyl cyclase pathway, was also significantly attenuated in Pkd2SM-CKO mice compared with that in wild-type mice. Carbachol 31-40 H19 imprinted maternally expressed transcript Homo sapiens 55-58 31411061-8 2019 In addition, relaxation of the carbachol-precontracted ASM by isoprenaline, a beta-adrenergic receptor agonist that acts through the cAMP/adenylyl cyclase pathway, was also significantly attenuated in Pkd2SM-CKO mice compared with that in wild-type mice. Carbachol 31-40 polycystin 2, transient receptor potential cation channel Mus musculus 201-207 31921474-5 2019 Sera from curdlan-injected SKG mice contained elevated titers of IgG (predominantly IgG1), autoantibody to the muscarinic type 3 receptor (M3R) and inhibited carbachol-induced Ca2+ signaling in salivary acinar cells. Carbachol 158-167 immunoglobulin heavy constant gamma 1 (G1m marker) Mus musculus 65-68 31260679-8 2019 The results showed that OX1R and OX2R antagonists dose-dependently decreased the antinociceptive effect of carbachol. Carbachol 107-116 hypocretin receptor 2 Rattus norvegicus 33-37 31260679-9 2019 In addition, the effective dose of SB334867 was lesser than that of TCS OX2 29, which indicates the more prominent role of OX1R of the DG in carbachol-induced antinociception. Carbachol 141-150 hypocretin receptor 1 Rattus norvegicus 123-127 30697954-6 2019 The Rac1 inhibitor EHT1864 inhibited carbachol (CCh)-induced BSM contractions, although high K+ depolarization-induced BSM contractions were not significantly attenuated by EHT1864. Carbachol 37-46 Rac family small GTPase 1 Mus musculus 4-8 31259654-5 2019 In rat tracheal rings, addition of both naringin and naringenin could concentration dependently relax carbachol (CCh)-evoked tonic contraction. Carbachol 102-111 neurogenin 3 Rattus norvegicus 96-101 31259654-5 2019 In rat tracheal rings, addition of both naringin and naringenin could concentration dependently relax carbachol (CCh)-evoked tonic contraction. Carbachol 113-116 neurogenin 3 Rattus norvegicus 96-101 31422097-9 2019 In addition, carbachol-induced contraction was reduced in isolated detrusor strips from the L-NAME group, and bladder expression of HIF-1 and connexin 43 showed upregulation. Carbachol 13-22 gap junction protein, alpha 1 Rattus norvegicus 142-153 31243195-0 2019 Increased Rac1 Activation in the Enhanced Carbachol-Induced Bronchial Smooth Muscle Contraction of Repeatedly Antigen-Challenged Mice. Carbachol 42-51 Rac family small GTPase 1 Mus musculus 10-14 31243195-4 2019 We here examined carbachol (CCh)-induced Rac1 activation in BSMs of Chal. Carbachol 17-26 Rac family small GTPase 1 Mus musculus 41-45 31243195-4 2019 We here examined carbachol (CCh)-induced Rac1 activation in BSMs of Chal. Carbachol 28-31 Rac family small GTPase 1 Mus musculus 41-45 31243195-8 2019 Furthermore, CCh-induced Rac1 activation was inhibited by pretreatment with Rac1-GEF inhibitor NSC23766 and Rac1 inhibitor EHT1864 in BSMs of sensitized-control (S.C.) and Chal. Carbachol 13-16 Rac family small GTPase 1 Mus musculus 25-29 31243195-8 2019 Furthermore, CCh-induced Rac1 activation was inhibited by pretreatment with Rac1-GEF inhibitor NSC23766 and Rac1 inhibitor EHT1864 in BSMs of sensitized-control (S.C.) and Chal. Carbachol 13-16 Rac family small GTPase 1 Mus musculus 76-80 31243195-8 2019 Furthermore, CCh-induced Rac1 activation was inhibited by pretreatment with Rac1-GEF inhibitor NSC23766 and Rac1 inhibitor EHT1864 in BSMs of sensitized-control (S.C.) and Chal. Carbachol 13-16 rho/rac guanine nucleotide exchange factor (GEF) 2 Mus musculus 81-84 31243195-8 2019 Furthermore, CCh-induced Rac1 activation was inhibited by pretreatment with Rac1-GEF inhibitor NSC23766 and Rac1 inhibitor EHT1864 in BSMs of sensitized-control (S.C.) and Chal. Carbachol 13-16 Rac family small GTPase 1 Mus musculus 76-80 31243195-10 2019 Compared with S.C. mice, CCh-induced Rac1 activation was increased in BSMs of Chal. Carbachol 25-28 Rac family small GTPase 1 Mus musculus 37-41 31243195-12 2019 In conclusion, we reported that increased CCh-induced Rac1 activation via Tiam1 and Trio upregulation, in addition to upregulate Rac1, may be involved in increased CCh-induced BSM contractions in Chal. Carbachol 42-45 Rac family small GTPase 1 Mus musculus 54-58 31243195-12 2019 In conclusion, we reported that increased CCh-induced Rac1 activation via Tiam1 and Trio upregulation, in addition to upregulate Rac1, may be involved in increased CCh-induced BSM contractions in Chal. Carbachol 42-45 T cell lymphoma invasion and metastasis 1 Mus musculus 74-79 31243195-12 2019 In conclusion, we reported that increased CCh-induced Rac1 activation via Tiam1 and Trio upregulation, in addition to upregulate Rac1, may be involved in increased CCh-induced BSM contractions in Chal. Carbachol 42-45 triple functional domain (PTPRF interacting) Mus musculus 84-88 31243195-12 2019 In conclusion, we reported that increased CCh-induced Rac1 activation via Tiam1 and Trio upregulation, in addition to upregulate Rac1, may be involved in increased CCh-induced BSM contractions in Chal. Carbachol 42-45 Rac family small GTPase 1 Mus musculus 129-133 31243195-12 2019 In conclusion, we reported that increased CCh-induced Rac1 activation via Tiam1 and Trio upregulation, in addition to upregulate Rac1, may be involved in increased CCh-induced BSM contractions in Chal. Carbachol 164-167 Rac family small GTPase 1 Mus musculus 54-58 31243195-12 2019 In conclusion, we reported that increased CCh-induced Rac1 activation via Tiam1 and Trio upregulation, in addition to upregulate Rac1, may be involved in increased CCh-induced BSM contractions in Chal. Carbachol 164-167 T cell lymphoma invasion and metastasis 1 Mus musculus 74-79 31243195-12 2019 In conclusion, we reported that increased CCh-induced Rac1 activation via Tiam1 and Trio upregulation, in addition to upregulate Rac1, may be involved in increased CCh-induced BSM contractions in Chal. Carbachol 164-167 triple functional domain (PTPRF interacting) Mus musculus 84-88 31243195-12 2019 In conclusion, we reported that increased CCh-induced Rac1 activation via Tiam1 and Trio upregulation, in addition to upregulate Rac1, may be involved in increased CCh-induced BSM contractions in Chal. Carbachol 164-167 Rac family small GTPase 1 Mus musculus 129-133 31227218-4 2019 When SNU-407 cells were treated with the cholinergic agonist carbachol, eEF2 phosphorylation at T56 was decreased in a dose- and time-dependent manner. Carbachol 61-70 eukaryotic translation elongation factor 2 Homo sapiens 72-76 31227218-5 2019 The muscarinic antagonist atropine almost completely blocked this effect of carbachol, demonstrating that mAChRs specifically regulate eEF2 dephosphorylation. Carbachol 76-85 eukaryotic translation elongation factor 2 Homo sapiens 135-139 31227218-7 2019 Treating cells with U0126, a potent MEK1/2 inhibitor, decreased carbachol-stimulated eEF2 dephosphorylation. Carbachol 64-73 mitogen-activated protein kinase kinase 1 Homo sapiens 36-42 31227218-7 2019 Treating cells with U0126, a potent MEK1/2 inhibitor, decreased carbachol-stimulated eEF2 dephosphorylation. Carbachol 64-73 eukaryotic translation elongation factor 2 Homo sapiens 85-89 31383845-0 2019 Anoctamin 1/TMEM16A controls intestinal Cl- secretion induced by carbachol and cholera toxin. Carbachol 65-74 anoctamin 1, calcium activated chloride channel Mus musculus 0-11 31383845-0 2019 Anoctamin 1/TMEM16A controls intestinal Cl- secretion induced by carbachol and cholera toxin. Carbachol 65-74 anoctamin 1, calcium activated chloride channel Mus musculus 12-19 31383845-3 2019 Although genetic ablation of ANO1/TMEM16A, a gene encoding a CaCC, reduces the carbachol-induced secretion of intestinal fluid, its mechanism of action is still unknown. Carbachol 79-88 anoctamin 1, calcium activated chloride channel Mus musculus 29-33 31383845-3 2019 Although genetic ablation of ANO1/TMEM16A, a gene encoding a CaCC, reduces the carbachol-induced secretion of intestinal fluid, its mechanism of action is still unknown. Carbachol 79-88 anoctamin 1, calcium activated chloride channel Mus musculus 34-41 31383845-3 2019 Although genetic ablation of ANO1/TMEM16A, a gene encoding a CaCC, reduces the carbachol-induced secretion of intestinal fluid, its mechanism of action is still unknown. Carbachol 79-88 chloride channel accessory 3A1 Mus musculus 61-65 31383845-5 2019 Carbachol-induced transepithelial currents were reduced in the proximal colon of Ano1-deficient mice. Carbachol 0-9 anoctamin 1, calcium activated chloride channel Mus musculus 81-85 31383845-10 2019 We thus conclude that ANO1 is necessary for cAMP- and carbachol-induced Cl- secretion in the intestine, which is essential for the protection of the intestinal epithelium from colitis. Carbachol 54-63 anoctamin 1, calcium activated chloride channel Mus musculus 22-26 30742476-4 2019 Overnight TGF-beta1 treatment significantly impaired isoproterenol (ISO)-induced relaxation of carbachol-stimulated, isolated HASM cells. Carbachol 95-104 transforming growth factor beta 1 Homo sapiens 10-19 30697954-6 2019 The Rac1 inhibitor EHT1864 inhibited carbachol (CCh)-induced BSM contractions, although high K+ depolarization-induced BSM contractions were not significantly attenuated by EHT1864. Carbachol 48-51 Rac family small GTPase 1 Mus musculus 4-8 31048413-3 2019 We report here that secretagogues (agonists that stimulate secretion) such as the peptide hormone vasoactive intestinal peptide (VIP) and muscarinic agonist carbachol increase CFTR aggregation into cholesterol-dependent clusters, reduce CFTR lateral mobility within and between membrane microdomains, and trigger the fusion of clusters into large (3.0 microm2) ceramide-rich platforms. Carbachol 157-166 CF transmembrane conductance regulator Homo sapiens 176-180 31042424-5 2019 The beta3-AR agonists BRL37344 and CL316243 (100 nM) inhibited cholinergic nerve-mediated contractions of the detrusor by 19 and 23%, respectively, but did not reduce contractions induced by the cholinergic agonist carbachol (300 nM). Carbachol 215-224 adrenergic receptor, beta 3 Mus musculus 4-12 31048413-3 2019 We report here that secretagogues (agonists that stimulate secretion) such as the peptide hormone vasoactive intestinal peptide (VIP) and muscarinic agonist carbachol increase CFTR aggregation into cholesterol-dependent clusters, reduce CFTR lateral mobility within and between membrane microdomains, and trigger the fusion of clusters into large (3.0 microm2) ceramide-rich platforms. Carbachol 157-166 CF transmembrane conductance regulator Homo sapiens 237-241 30382364-11 2019 Deletion of IP3R1 in the GI tract also reduced the contractile response to the muscarinic agonist, carbachol, as well as to electrical field stimulation. Carbachol 99-108 inositol 1,4,5-trisphosphate receptor 1 Mus musculus 12-17 30120731-4 2019 Mice with a single copy of Gtf2i (Gtf2i+/Del) had increased axonal outgrowth and increased TRPC3-mediated calcium entry upon carbachol stimulation. Carbachol 125-134 general transcription factor II I Mus musculus 27-32 30840492-4 2019 Costimulation with carbachol plus the beta-adrenergic agonist isoproterenol decreased the concentration of NaCl and produced a substantial increase in the protein and Ca2+ content of SMG but not SLG saliva, consistent with a sparse sympathetic innervation of the SLGs. Carbachol 19-28 small nuclear ribonucleoprotein polypeptide G Mus musculus 183-186 30120731-4 2019 Mice with a single copy of Gtf2i (Gtf2i+/Del) had increased axonal outgrowth and increased TRPC3-mediated calcium entry upon carbachol stimulation. Carbachol 125-134 general transcription factor II I Mus musculus 34-39 30120731-4 2019 Mice with a single copy of Gtf2i (Gtf2i+/Del) had increased axonal outgrowth and increased TRPC3-mediated calcium entry upon carbachol stimulation. Carbachol 125-134 transient receptor potential cation channel, subfamily C, member 3 Mus musculus 91-96 31008485-5 2019 The results showed that, the slope of field excitatory postsynaptic potential (fEPSP) and carbachol-induced theta oscillation were significantly decreased in the hippocampal CA1 neurons of alpha7 KO mice, compared with those of wild type mice. Carbachol 90-99 carbonic anhydrase 1 Mus musculus 174-177 30872401-4 2019 Here, we found that in human neuroblastoma cells, activation of ADAM10 with the muscarinic agonist carbachol promotes PrPC shedding and reduces the binding of AbetaO to the cell surface, which could be blocked with an ADAM10 inhibitor. Carbachol 99-108 ADAM metallopeptidase domain 10 Homo sapiens 64-70 30872401-4 2019 Here, we found that in human neuroblastoma cells, activation of ADAM10 with the muscarinic agonist carbachol promotes PrPC shedding and reduces the binding of AbetaO to the cell surface, which could be blocked with an ADAM10 inhibitor. Carbachol 99-108 prion protein Homo sapiens 118-122 30872401-4 2019 Here, we found that in human neuroblastoma cells, activation of ADAM10 with the muscarinic agonist carbachol promotes PrPC shedding and reduces the binding of AbetaO to the cell surface, which could be blocked with an ADAM10 inhibitor. Carbachol 99-108 ADAM metallopeptidase domain 10 Homo sapiens 218-224 30639512-13 2019 Finally, carbachol induced secretion of exosomal NEP in C2C12-derived myotube cells. Carbachol 9-18 membrane metallo endopeptidase Mus musculus 49-52 31040768-7 2019 In the current study, an ex vivo drug treatment assay was used to demonstrate that carbachol (CCh)-mediated cholinergic stimulation of the intact adult zebrafish brain induces phosphorylation of GSK3beta and ERK1/2 in the zebrafish telencephalon. Carbachol 83-92 glycogen synthase kinase 3 beta, genome duplicate a Danio rerio 195-203 31040768-7 2019 In the current study, an ex vivo drug treatment assay was used to demonstrate that carbachol (CCh)-mediated cholinergic stimulation of the intact adult zebrafish brain induces phosphorylation of GSK3beta and ERK1/2 in the zebrafish telencephalon. Carbachol 83-92 mitogen-activated protein kinase 3 Danio rerio 208-214 31040768-7 2019 In the current study, an ex vivo drug treatment assay was used to demonstrate that carbachol (CCh)-mediated cholinergic stimulation of the intact adult zebrafish brain induces phosphorylation of GSK3beta and ERK1/2 in the zebrafish telencephalon. Carbachol 94-97 glycogen synthase kinase 3 beta, genome duplicate a Danio rerio 195-203 31040768-7 2019 In the current study, an ex vivo drug treatment assay was used to demonstrate that carbachol (CCh)-mediated cholinergic stimulation of the intact adult zebrafish brain induces phosphorylation of GSK3beta and ERK1/2 in the zebrafish telencephalon. Carbachol 94-97 mitogen-activated protein kinase 3 Danio rerio 208-214 30223917-4 2018 Here we present a reaction for identification of carbamate-type drugs, based on the precipitation of barium carbonate upon treating the analytes with barium hydroxide solution at 80 C. This method works well for carbamate drugs with noteworthy water solubility like carbachol, neostigmine bromide, and pyridostigmine bromide, and could be considered as a method for second identification of these drugs in pharmacopoeias and in Deutscher Arzneimittel-Codex/Neues Rezeptur-Formularium (DAC-NRF). Carbachol 267-276 arylacetamide deacetylase Homo sapiens 486-489 30632283-7 2019 RESULTS: 30 muM Mirabegron impaired carbachol (0.03-1 muM)-induced contraction in human detrusor smooth muscle. Carbachol 36-45 latexin Homo sapiens 12-15 30632283-7 2019 RESULTS: 30 muM Mirabegron impaired carbachol (0.03-1 muM)-induced contraction in human detrusor smooth muscle. Carbachol 36-45 latexin Homo sapiens 54-57 30407877-4 2019 When the mAChR was activated by the agonist carbachol, the colocalization of the M1 mAChR and SUMO-1 protein markedly decreased in immunoprecipitation and immunofluorescence assays. Carbachol 44-53 small ubiquitin like modifier 1 Homo sapiens 94-100 29927500-7 2018 To pursue how 7,8-DHF could augment CCh-activated PLC-gamma phosphorylation, we first examined the effect of 7,8-DHF on the expression of muscarinic receptors in gastric muscle and found that 7,8-DHF specifically increased M3 but not M2 receptor expression possibly through TrkB/Akt (protein kinase B) pathway because the Akt antagonist, LY294002 significantly suppressed the 7,8-DHF-augmemted M3 expression and completely blocked the 7,8-DHF-enhanced cholinergic contraction. Carbachol 36-39 neurotrophic receptor tyrosine kinase 2 Rattus norvegicus 274-278 29927500-7 2018 To pursue how 7,8-DHF could augment CCh-activated PLC-gamma phosphorylation, we first examined the effect of 7,8-DHF on the expression of muscarinic receptors in gastric muscle and found that 7,8-DHF specifically increased M3 but not M2 receptor expression possibly through TrkB/Akt (protein kinase B) pathway because the Akt antagonist, LY294002 significantly suppressed the 7,8-DHF-augmemted M3 expression and completely blocked the 7,8-DHF-enhanced cholinergic contraction. Carbachol 36-39 AKT serine/threonine kinase 1 Rattus norvegicus 279-282 29927500-7 2018 To pursue how 7,8-DHF could augment CCh-activated PLC-gamma phosphorylation, we first examined the effect of 7,8-DHF on the expression of muscarinic receptors in gastric muscle and found that 7,8-DHF specifically increased M3 but not M2 receptor expression possibly through TrkB/Akt (protein kinase B) pathway because the Akt antagonist, LY294002 significantly suppressed the 7,8-DHF-augmemted M3 expression and completely blocked the 7,8-DHF-enhanced cholinergic contraction. Carbachol 36-39 AKT serine/threonine kinase 1 Rattus norvegicus 322-325 30311722-7 2019 In vitro, the RET kinase inhibitor GSK3179106 attenuated the mean increase in Isc induced by either EFS or carbachol but not bethanechol. Carbachol 107-116 ret proto-oncogene Rattus norvegicus 14-17 30798915-11 2019 Various compounds that accelerate emergence from anesthesia, thus mitigating problematic effects associated with delayed emergence such as delirium, also activate AMPK (e.g. nicotine, caffeine, forskolin, carbachol). Carbachol 205-214 protein kinase AMP-activated catalytic subunit alpha 1 Homo sapiens 163-167 30886671-4 2019 We have found that H2O2 in concentration range 10-100 muM increases the rise of [Ca2+]i induced by 5-hydroxytryptamine (5-HT) and carbachol and does not affect the calcium signals of ATP, agonist of type 1 protease-activated receptor SFLLRN, histamine and bradykinin. Carbachol 130-139 latexin Homo sapiens 54-57 30834352-5 2019 In CA1 minislices isolated from rat ventral hippocampus slices, MOR activation by DAMGO reduced the dominant frequency of intrinsic fast gamma, induced by carbachol. Carbachol 155-164 carbonic anhydrase 1 Rattus norvegicus 3-6 30203559-5 2019 Our results showed that high-K+ - or carbachol-induced contractions of mouse ASM were significantly greater after pretreatment with TNF-alpha or IL-8 for 24 hours. Carbachol 37-46 tumor necrosis factor Mus musculus 132-141 30203559-5 2019 Our results showed that high-K+ - or carbachol-induced contractions of mouse ASM were significantly greater after pretreatment with TNF-alpha or IL-8 for 24 hours. Carbachol 37-46 chemokine (C-X-C motif) ligand 15 Mus musculus 145-149 30203559-7 2019 Moreover, TNF-alpha treatment enhanced carbachol-induced Ca2+ influx in ASM cells, and this effect was abrogated by verapamil. Carbachol 39-48 tumor necrosis factor Mus musculus 10-19 29959979-3 2018 The cholinergic agonists carbachol and acetylcholine triggered heterogeneous Ca2+ oscillations that were strongly inhibited by antagonists with high affinity for the M3 muscarinic receptor subtype, while preferential block of M1 or M2 receptors was less effective. Carbachol 25-34 cholinergic receptor muscarinic 3 Homo sapiens 166-188 30618761-7 2018 Carbachol raised [Ca2+]cyt in mouse tracheal smooth muscle cells and induced murine tracheal contraction, all of which were significantly attenuated by KB-R7943, a selective NCX inhibitor. Carbachol 0-9 T cell leukemia, homeobox 2 Mus musculus 174-177 30160518-5 2018 Phosphorylation of MLC was measured after having been stimulated by carbachol. Carbachol 68-77 modulator of VRAC current 1 Homo sapiens 19-22 30001145-8 2018 The Gq/11 inhibitor YM-254890 (10 muM) and PLC inhibitor U73122 (1 muM), but not the PKC inhibitor calphostin C (1 muM), markedly decreased CCh-induced suppression of IKATP in WT cells. Carbachol 140-143 perlecan (heparan sulfate proteoglycan 2) Mus musculus 43-46 30223917-4 2018 Here we present a reaction for identification of carbamate-type drugs, based on the precipitation of barium carbonate upon treating the analytes with barium hydroxide solution at 80 C. This method works well for carbamate drugs with noteworthy water solubility like carbachol, neostigmine bromide, and pyridostigmine bromide, and could be considered as a method for second identification of these drugs in pharmacopoeias and in Deutscher Arzneimittel-Codex/Neues Rezeptur-Formularium (DAC-NRF). Carbachol 267-276 NFKB repressing factor Homo sapiens 490-493 30249045-8 2018 Finally, we used telemetry to monitor heart response to carbachol, a cholinergic receptor agonist, and found that heart rate recovered more quickly in Tbx1+/- animals versus controls. Carbachol 56-65 T-box 1 Mus musculus 151-155 29561662-4 2018 Therefore, we hypothesized that decreased carbachol (CCH)-stimulated Cl- secretion in DSS-induced colitis could be attributed to a loss of Ca2+-activated Cl- channels (CaCC) in apical membranes of colonic epithelium. Carbachol 42-51 chloride channel accessory 3A1 Mus musculus 139-166 29561662-4 2018 Therefore, we hypothesized that decreased carbachol (CCH)-stimulated Cl- secretion in DSS-induced colitis could be attributed to a loss of Ca2+-activated Cl- channels (CaCC) in apical membranes of colonic epithelium. Carbachol 42-51 chloride channel accessory 3A1 Mus musculus 168-172 29561662-4 2018 Therefore, we hypothesized that decreased carbachol (CCH)-stimulated Cl- secretion in DSS-induced colitis could be attributed to a loss of Ca2+-activated Cl- channels (CaCC) in apical membranes of colonic epithelium. Carbachol 53-56 chloride channel accessory 3A1 Mus musculus 139-166 29561662-4 2018 Therefore, we hypothesized that decreased carbachol (CCH)-stimulated Cl- secretion in DSS-induced colitis could be attributed to a loss of Ca2+-activated Cl- channels (CaCC) in apical membranes of colonic epithelium. Carbachol 53-56 chloride channel accessory 3A1 Mus musculus 168-172 29471582-7 2018 A significant reduction was observed in STZ-diabetic aortic endothelial cell eNOS and CAV-1 of 40% and 30%, respectively, accompanied by a compromised STZ-diabetic carbachol-induced vasodilation (STZ 29.6 +- 9.3% vs control 77.2 +- 2.5%, P < .001). Carbachol 164-173 nitric oxide synthase 3 Rattus norvegicus 77-81 29471582-7 2018 A significant reduction was observed in STZ-diabetic aortic endothelial cell eNOS and CAV-1 of 40% and 30%, respectively, accompanied by a compromised STZ-diabetic carbachol-induced vasodilation (STZ 29.6 +- 9.3% vs control 77.2 +- 2.5%, P < .001). Carbachol 164-173 caveolin 1 Rattus norvegicus 86-91 29668674-4 2018 Consistent with previous findings, mice lacking GIRK4 exhibited diminished HR and HRV responses to the cholinergic agonist carbachol (CCh), and resistance to CCh-induced arrhythmic episodes. Carbachol 123-132 potassium inwardly-rectifying channel, subfamily J, member 5 Mus musculus 48-53 29527918-8 2018 In carbachol pre-stimulated ciliary muscle, UCN2 (10(-5) M) enhanced contraction (maximal effect of 18.2 % increase vs. control). Carbachol 3-12 urocortin 2 Bos taurus 44-48 29527918-10 2018 Regarding ciliary muscle, UCN2 enhances carbachol-induced contraction, in higher doses. Carbachol 40-49 urocortin 2 Bos taurus 26-30 29211339-0 2018 beta3-adrenoceptor agonists inhibit carbachol-evoked Ca2+ oscillations in murine detrusor myocytes. Carbachol 36-45 adrenergic receptor, beta 3 Mus musculus 0-18 29211339-6 2018 The selective beta3-AR agonist BRL37344 reduced the amplitude of CCh-induced contractions of detrusor smooth muscle. Carbachol 65-68 adrenergic receptor, beta 3 Mus musculus 14-22 29676804-7 2018 The CCh-induced salivary flow was suppressed by phlorizin, an inhibitor of the sodium-glucose cotransporter 1 (SGLT1) located basolaterally in submandibular acinar cells, which is altered at the protein expression level in diabetic animal models. Carbachol 4-7 solute carrier family 5 (sodium/glucose cotransporter), member 1 Mus musculus 79-109 29676804-7 2018 The CCh-induced salivary flow was suppressed by phlorizin, an inhibitor of the sodium-glucose cotransporter 1 (SGLT1) located basolaterally in submandibular acinar cells, which is altered at the protein expression level in diabetic animal models. Carbachol 4-7 solute carrier family 5 (sodium/glucose cotransporter), member 1 Mus musculus 111-116 28984468-5 2018 In human precision-cut lung slices, overnight treatment with TGF-beta1 significantly augmented basal and carbachol-induced bronchoconstriction. Carbachol 105-114 transforming growth factor beta 1 Homo sapiens 61-70 29401590-7 2018 Activation of Galphaq by carbachol causes movement of PLCbeta from the cytosol to the plasma membrane, reducing its association with CDK16. Carbachol 25-34 G protein subunit alpha q Homo sapiens 14-21 29401590-7 2018 Activation of Galphaq by carbachol causes movement of PLCbeta from the cytosol to the plasma membrane, reducing its association with CDK16. Carbachol 25-34 cyclin dependent kinase 16 Homo sapiens 133-138 29430647-8 2018 Drugs that block Ano1 inhibited the conductance activated by carbachol in ICC-IM and EJPs and mechanical responses in tissues. Carbachol 61-70 anoctamin 1, calcium activated chloride channel Mus musculus 17-21 29792810-5 2018 RGS2 reduced the stochasticity of carbachol-stimulated calcium oscillations, and the feedback inhibition was coupled to the global calcium elevation by calmodulin/RGS2 interactions. Carbachol 34-43 regulator of G protein signaling 2 Homo sapiens 0-4 29915209-6 2018 Cholinergic responses, induced by electric-field stimulation (EFS), bath application of the cholinergic agonist carbachol, or the acetylcholinesterase inhibitor neostigmine were all significantly smaller in TRPC4-/- detrusor strips than wild-type. Carbachol 112-121 transient receptor potential cation channel, subfamily C, member 4 Mus musculus 207-212 29668674-4 2018 Consistent with previous findings, mice lacking GIRK4 exhibited diminished HR and HRV responses to the cholinergic agonist carbachol (CCh), and resistance to CCh-induced arrhythmic episodes. Carbachol 134-137 potassium inwardly-rectifying channel, subfamily J, member 5 Mus musculus 48-53 29668674-4 2018 Consistent with previous findings, mice lacking GIRK4 exhibited diminished HR and HRV responses to the cholinergic agonist carbachol (CCh), and resistance to CCh-induced arrhythmic episodes. Carbachol 158-161 potassium inwardly-rectifying channel, subfamily J, member 5 Mus musculus 48-53 29668674-5 2018 In line with its role as a negative regulator of atrial M2R-IKACh signaling, loss of RGS6 correlated with a mild resting bradycardia, enhanced HR and HRV responses to CCh, and increased propensity for arrhythmic episodes. Carbachol 167-170 regulator of G-protein signaling 6 Mus musculus 85-89 29668674-6 2018 Interestingly, ventricles from mice lacking GIRK4 or RGS6 both exhibited increased action potential duration (APD) at baseline, and APD was prolonged by CCh across all genotypes. Carbachol 153-156 potassium inwardly-rectifying channel, subfamily J, member 5 Mus musculus 44-49 29463029-6 2018 HEK293 cells deficient in a penta-EF-hand Ca2+-binding protein ALG-2 showed a higher RLA value than the parental cells by stimulation with an acetylcholine receptor agonist carbachol. Carbachol 173-182 ALG2 alpha-1,3/1,6-mannosyltransferase Homo sapiens 63-68 29055784-5 2018 Our results demonstrated that muscle contractions induced by carbachol (CCh) and endothelin-1 (ET-1) were inhibited by EHT1864, a selective Rac1 inhibitor, and NSC23766, a selective inhibitor of Rac1-specific guanine nucleotide exchange factors. Carbachol 61-70 Rac family small GTPase 1 Rattus norvegicus 140-144 29352184-4 2018 The cholinergic agonist carbachol (CCh) activated small GIRK currents in adult wild-type ventricular myocytes that exhibited relatively slow kinetics and low CCh sensitivity; these currents were absent in ventricular myocytes from Girk1-/- or Girk4-/- mice. Carbachol 24-33 potassium inwardly-rectifying channel, subfamily J, member 3 Mus musculus 231-236 29055784-5 2018 Our results demonstrated that muscle contractions induced by carbachol (CCh) and endothelin-1 (ET-1) were inhibited by EHT1864, a selective Rac1 inhibitor, and NSC23766, a selective inhibitor of Rac1-specific guanine nucleotide exchange factors. Carbachol 61-70 Rac family small GTPase 1 Rattus norvegicus 195-199 29352184-4 2018 The cholinergic agonist carbachol (CCh) activated small GIRK currents in adult wild-type ventricular myocytes that exhibited relatively slow kinetics and low CCh sensitivity; these currents were absent in ventricular myocytes from Girk1-/- or Girk4-/- mice. Carbachol 24-33 potassium inwardly-rectifying channel, subfamily J, member 5 Mus musculus 243-248 29352184-4 2018 The cholinergic agonist carbachol (CCh) activated small GIRK currents in adult wild-type ventricular myocytes that exhibited relatively slow kinetics and low CCh sensitivity; these currents were absent in ventricular myocytes from Girk1-/- or Girk4-/- mice. Carbachol 35-38 potassium inwardly-rectifying channel, subfamily J, member 3 Mus musculus 231-236 29352184-4 2018 The cholinergic agonist carbachol (CCh) activated small GIRK currents in adult wild-type ventricular myocytes that exhibited relatively slow kinetics and low CCh sensitivity; these currents were absent in ventricular myocytes from Girk1-/- or Girk4-/- mice. Carbachol 35-38 potassium inwardly-rectifying channel, subfamily J, member 5 Mus musculus 243-248 29122814-7 2018 We found that arteries from EC Calr Delta/Delta mice stimulated with CCh had unchanged activity of calcium signals and vasodilation; however, the same arteries were unable to increase calcium events at HIEL in response to PE. Carbachol 69-72 calreticulin Mus musculus 31-35 29055784-5 2018 Our results demonstrated that muscle contractions induced by carbachol (CCh) and endothelin-1 (ET-1) were inhibited by EHT1864, a selective Rac1 inhibitor, and NSC23766, a selective inhibitor of Rac1-specific guanine nucleotide exchange factors. Carbachol 72-75 Rac family small GTPase 1 Rattus norvegicus 140-144 29055784-5 2018 Our results demonstrated that muscle contractions induced by carbachol (CCh) and endothelin-1 (ET-1) were inhibited by EHT1864, a selective Rac1 inhibitor, and NSC23766, a selective inhibitor of Rac1-specific guanine nucleotide exchange factors. Carbachol 72-75 Rac family small GTPase 1 Rattus norvegicus 195-199 29055784-11 2018 These results suggest that Rac1, activated by G protein-coupled receptor agonists, such as CCh and ET-1, may induce myosin light chain and MYPT phosphorylation and modulate the contraction of bronchial smooth muscle. Carbachol 91-94 Rac family small GTPase 1 Rattus norvegicus 27-31 28972132-6 2017 LRRC8B-overexpressing cells exhibited a lesser release of Ca2+ from the ER in response to ATP, carbachol and intracellular administration of inositol (1,4,5)-trisphosphate (IP3). Carbachol 95-104 leucine rich repeat containing 8 VRAC subunit B Homo sapiens 0-6 29042271-4 2018 Intra-LH microinjection of carbachol was done 5min after intra-CA1 administration of SB-334867 (OX1R antagonist) or TCS OX2 29 (OX2R antagonist). Carbachol 27-36 hypocretin receptor 1 Rattus norvegicus 96-100 29042438-5 2017 In isolated pancreatic lobules, pretreatment with recombinant human renalase (rRNLS) blocked zymogen activation caused by cerulein, carbachol, and a bile acid. Carbachol 132-141 renalase, FAD dependent amine oxidase Homo sapiens 68-76 29270134-2 2017 Our previous in vitro studies with molecular approaches on AR42J cell showed that protein kinase D (PKD/PKD1) activation was required in NF-kappaB activation induced by cholecystokinin 8 (CCK) or carbachol (CCh) in pancreatic acinar cells. Carbachol 196-205 polycystin 1, transient receptor potential channel interacting Rattus norvegicus 104-108 29270134-2 2017 Our previous in vitro studies with molecular approaches on AR42J cell showed that protein kinase D (PKD/PKD1) activation was required in NF-kappaB activation induced by cholecystokinin 8 (CCK) or carbachol (CCh) in pancreatic acinar cells. Carbachol 207-210 polycystin 1, transient receptor potential channel interacting Rattus norvegicus 104-108 29270134-2 2017 Our previous in vitro studies with molecular approaches on AR42J cell showed that protein kinase D (PKD/PKD1) activation was required in NF-kappaB activation induced by cholecystokinin 8 (CCK) or carbachol (CCh) in pancreatic acinar cells. Carbachol 207-210 cholecystokinin Rattus norvegicus 188-191 29208957-7 2017 In isolated islets, carbachol and PACAP/VIP synergistically promote beta-cell proliferation through a FoxM1-dependent mechanism. Carbachol 20-29 forkhead box M1 Mus musculus 102-107 28921504-10 2017 Galpha12 coimmunoprecipitated with M3 receptors, and p115RhoGEF-RGS overexpression inhibited carbachol-mediated induction of SRE-luciferase reporter. Carbachol 93-102 Rho guanine nucleotide exchange factor 1 Homo sapiens 53-63 28921504-10 2017 Galpha12 coimmunoprecipitated with M3 receptors, and p115RhoGEF-RGS overexpression inhibited carbachol-mediated induction of SRE-luciferase reporter. Carbachol 93-102 paired like homeodomain 2 Homo sapiens 64-67 28921504-11 2017 p115RhoGEF-RGS overexpression inhibited carbachol-induced activation of Akt, HASMC contraction, and shortening. Carbachol 40-49 Rho guanine nucleotide exchange factor 1 Homo sapiens 0-10 28921504-11 2017 p115RhoGEF-RGS overexpression inhibited carbachol-induced activation of Akt, HASMC contraction, and shortening. Carbachol 40-49 paired like homeodomain 2 Homo sapiens 11-14 28921504-11 2017 p115RhoGEF-RGS overexpression inhibited carbachol-induced activation of Akt, HASMC contraction, and shortening. Carbachol 40-49 AKT serine/threonine kinase 1 Homo sapiens 72-75 29142468-10 2017 In the presence of guanethidine and carbachol, the addition of GLP-2 to the bath medium evoked TTX-sensitive relaxant responses that were unaffected by L-NNA (P > 0.05). Carbachol 36-45 glucagon-like peptide 2 receptor Mus musculus 63-68 28893976-6 2017 Stimulation of cells overexpressing M1R or M3R with CCh resulted in a similar reduction in phosphatidylinositol 4,5-bisphosphate (PIP2). Carbachol 52-55 cholinergic receptor, muscarinic 1, CNS Mus musculus 36-39 28893976-6 2017 Stimulation of cells overexpressing M1R or M3R with CCh resulted in a similar reduction in phosphatidylinositol 4,5-bisphosphate (PIP2). Carbachol 52-55 cholinergic receptor, muscarinic 3, cardiac Mus musculus 43-46 28893976-8 2017 Moreover, pilocarpine blocked CCh-stimulated PIP2 hydrolysis in M3R-overexpressing cells, thus, it acted as an antagonist. Carbachol 30-33 cholinergic receptor, muscarinic 3, cardiac Mus musculus 64-67 28798231-7 2017 Lastly, in vivo administration of the P2X7R antagonist A438079 in the CD28-/-, IFNgamma-/-, NOD.H-2h4 mouse model of salivary gland exocrinopathy ameliorated salivary gland inflammation and enhanced carbachol-induced saliva secretion. Carbachol 199-208 purinergic receptor P2X, ligand-gated ion channel, 7 Mus musculus 38-43 28645101-0 2017 Co-localization of endogenous Arf6 and its activator EFA6D in the granular convoluted tubule cells of mouse submandibular glands under normal conditions and when stimulated by isoproterenol, noradrenaline and carbachol. Carbachol 209-218 ADP-ribosylation factor 6 Mus musculus 30-34 28971603-4 2017 Carbachol (CCh)-induced contraction in rabbit muscle strips and isolated muscle cells was inhibited by l-cysteine (substrate of CSE) and NaHS (an exogenous H2 S donor) in a concentration-dependent fashion. Carbachol 0-9 cystathionine gamma-lyase Oryctolagus cuniculus 128-131 28971603-4 2017 Carbachol (CCh)-induced contraction in rabbit muscle strips and isolated muscle cells was inhibited by l-cysteine (substrate of CSE) and NaHS (an exogenous H2 S donor) in a concentration-dependent fashion. Carbachol 11-14 cystathionine gamma-lyase Oryctolagus cuniculus 128-131 28971603-6 2017 CCh-induced Rho kinase activity also was inhibited by l-cysteine and NaHS in a concentration-dependent fashion. Carbachol 0-3 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 12-22 28971603-7 2017 Inhibition of CCh-induced contraction by l-cysteine was blocked by the CSE inhibitor, dl-propargylglycine (DL-PPG) in dispersed muscle cells. Carbachol 14-17 cystathionine gamma-lyase Oryctolagus cuniculus 71-74 28971603-7 2017 Inhibition of CCh-induced contraction by l-cysteine was blocked by the CSE inhibitor, dl-propargylglycine (DL-PPG) in dispersed muscle cells. Carbachol 14-17 serglycin Homo sapiens 110-113 28971603-8 2017 Inhibition of CCh-induced Rho kinase activity by l-cysteine was blocked by CSE siRNA in cultured cells and DL-PPG in dispersed muscle cells. Carbachol 14-17 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 26-36 28971603-8 2017 Inhibition of CCh-induced Rho kinase activity by l-cysteine was blocked by CSE siRNA in cultured cells and DL-PPG in dispersed muscle cells. Carbachol 14-17 cystathionine gamma-lyase Oryctolagus cuniculus 75-78 28971603-8 2017 Inhibition of CCh-induced Rho kinase activity by l-cysteine was blocked by CSE siRNA in cultured cells and DL-PPG in dispersed muscle cells. Carbachol 14-17 serglycin Homo sapiens 110-113 28971603-9 2017 Stimulation of Rho kinase activity and muscle contraction in response to CCh was also inhibited by l-cysteine or NaHS in colonic muscle cells from mouse and human. Carbachol 73-76 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 15-25 28749013-3 2017 Concentration/temporal responses to carbachol demonstrated similar sensitivities for bovine tracheal force development and phosphorylation of RLC, MYPT1, MBS85 and paxillin. Carbachol 36-45 protein phosphatase 1, regulatory subunit 12A Mus musculus 147-152 28749013-3 2017 Concentration/temporal responses to carbachol demonstrated similar sensitivities for bovine tracheal force development and phosphorylation of RLC, MYPT1, MBS85 and paxillin. Carbachol 36-45 protein phosphatase 1, regulatory subunit 12C Mus musculus 154-159 28645101-0 2017 Co-localization of endogenous Arf6 and its activator EFA6D in the granular convoluted tubule cells of mouse submandibular glands under normal conditions and when stimulated by isoproterenol, noradrenaline and carbachol. Carbachol 209-218 pleckstrin and Sec7 domain containing 3 Mus musculus 53-58 29033789-5 2017 We report that multiple, unpredictable exposure to stress depresses carbachol (0.5 muM)-induced mLTP, while this effect of stress is not observed in hippocampal slices prepared from mice exposed only to a single stressful procedure. Carbachol 68-77 liver transport protein Mus musculus 96-100 28966579-7 2017 Inhibition of the MS with muscimol pre-treatment attenuated both carbachol-evoked c-Fos-ir and theta activation. Carbachol 65-74 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 82-87 28902875-6 2017 Although total cellular cathepsin S activity was not commensurately increased, IFN-gamma treated lacrimal gland acinar cells showed a significant increase in carbachol-stimulated secretion of cathepsin S similar to the lacrimal gland in disease. Carbachol 158-167 interferon gamma Mus musculus 79-88 28902875-6 2017 Although total cellular cathepsin S activity was not commensurately increased, IFN-gamma treated lacrimal gland acinar cells showed a significant increase in carbachol-stimulated secretion of cathepsin S similar to the lacrimal gland in disease. Carbachol 158-167 cathepsin S Oryctolagus cuniculus 192-203 28495796-3 2017 NHE3-S719A but not -S719D had multiple changes in NHE3 activity: 1) reduced basal NHE3 activity-specifically, inhibition of the PI3K/AKT-dependent component; 2) reduced acute stimulation of NHE3 activity by LPA/LPA5R stimulation; and 3) reduced acute inhibition of NHE3 activity-specifically, elevated Ca2+ related (carbachol/Ca2+ ionophore), but there was normal inhibition by forskolin and hyperosmolarity. Carbachol 316-325 solute carrier family 9 member A3 Homo sapiens 50-54 28549258-0 2017 Immunohistochemical localization of cannabinoid receptor 1 (CB1) in the submandibular gland of mice under normal conditions and when stimulated by isoproterenol or carbachol. Carbachol 164-173 cannabinoid receptor 1 (brain) Mus musculus 36-58 28549258-0 2017 Immunohistochemical localization of cannabinoid receptor 1 (CB1) in the submandibular gland of mice under normal conditions and when stimulated by isoproterenol or carbachol. Carbachol 164-173 cannabinoid receptor 1 (brain) Mus musculus 60-63 28786172-8 2017 Electrophysiological results demonstrated that in the hippocampal CA3-CA1 synapses of young Apoe4 mice, basic synaptic transmission, and paired-pulse facilitation were enhanced but long-term potentiation and carbachol-induced hippocampal theta oscillations were impaired compared to young Apoe3 mice. Carbachol 208-217 carbonic anhydrase 3 Mus musculus 66-73 28786172-8 2017 Electrophysiological results demonstrated that in the hippocampal CA3-CA1 synapses of young Apoe4 mice, basic synaptic transmission, and paired-pulse facilitation were enhanced but long-term potentiation and carbachol-induced hippocampal theta oscillations were impaired compared to young Apoe3 mice. Carbachol 208-217 apolipoprotein E Homo sapiens 92-97 28687907-5 2017 Besides, the carbachol-stimulated [35S]GTPgammaS binding to Galphaq was competitively antagonized by telenzepine (with a pA 2 value of 8.81), indicating the involvement of the M1 muscarinic acetylcholine receptor (mAChR). Carbachol 13-22 G protein subunit alpha q Homo sapiens 60-67 28687907-8 2017 When the pharmacological parameters were correlated with age, postmortem delay, freezing storage period, and tissue pH, no statistically significant correlation was observed except for the negative correlation between age and %E max value of carbachol-stimulated [35S]GTPgammaS binding to Galphaq. Carbachol 242-251 G protein subunit alpha q Homo sapiens 289-296 28736134-7 2017 In neuroblastoma cells expressing epitope tagged-Arc, we demonstrate ERK-dependent phosphorylation of Arc in response to activation of muscarinic cholinergic receptors with carbachol. Carbachol 173-182 activity-regulated cytoskeleton-associated protein Rattus norvegicus 49-52 28736134-7 2017 In neuroblastoma cells expressing epitope tagged-Arc, we demonstrate ERK-dependent phosphorylation of Arc in response to activation of muscarinic cholinergic receptors with carbachol. Carbachol 173-182 Eph receptor B1 Rattus norvegicus 69-72 28736134-7 2017 In neuroblastoma cells expressing epitope tagged-Arc, we demonstrate ERK-dependent phosphorylation of Arc in response to activation of muscarinic cholinergic receptors with carbachol. Carbachol 173-182 activity-regulated cytoskeleton-associated protein Rattus norvegicus 102-105 28495999-10 2017 Potential beta-arrestin signaling-mediated increases in hERG and IKr were also observed in hERG-HEK cells as well as in neonatal rat ventricular myocytes treated with the muscarinic agonist carbachol. Carbachol 190-199 ETS transcription factor ERG Homo sapiens 56-60 28687079-8 2017 Cilostamide, a specific inhibitor for PDE3, significantly inhibited the amplitude and frequency of carbachol-enhanced phasic contractions of neonatal rat bladder strips by 38.8% and 12.1%, respectively. Carbachol 99-108 phosphodiesterase 4D, cAMP-specific-like 1 Rattus norvegicus 38-42 28495796-3 2017 NHE3-S719A but not -S719D had multiple changes in NHE3 activity: 1) reduced basal NHE3 activity-specifically, inhibition of the PI3K/AKT-dependent component; 2) reduced acute stimulation of NHE3 activity by LPA/LPA5R stimulation; and 3) reduced acute inhibition of NHE3 activity-specifically, elevated Ca2+ related (carbachol/Ca2+ ionophore), but there was normal inhibition by forskolin and hyperosmolarity. Carbachol 316-325 solute carrier family 9 member A3 Homo sapiens 0-4 28495796-3 2017 NHE3-S719A but not -S719D had multiple changes in NHE3 activity: 1) reduced basal NHE3 activity-specifically, inhibition of the PI3K/AKT-dependent component; 2) reduced acute stimulation of NHE3 activity by LPA/LPA5R stimulation; and 3) reduced acute inhibition of NHE3 activity-specifically, elevated Ca2+ related (carbachol/Ca2+ ionophore), but there was normal inhibition by forskolin and hyperosmolarity. Carbachol 316-325 solute carrier family 9 member A3 Homo sapiens 50-54 28495796-3 2017 NHE3-S719A but not -S719D had multiple changes in NHE3 activity: 1) reduced basal NHE3 activity-specifically, inhibition of the PI3K/AKT-dependent component; 2) reduced acute stimulation of NHE3 activity by LPA/LPA5R stimulation; and 3) reduced acute inhibition of NHE3 activity-specifically, elevated Ca2+ related (carbachol/Ca2+ ionophore), but there was normal inhibition by forskolin and hyperosmolarity. Carbachol 316-325 solute carrier family 9 member A3 Homo sapiens 50-54 28495796-3 2017 NHE3-S719A but not -S719D had multiple changes in NHE3 activity: 1) reduced basal NHE3 activity-specifically, inhibition of the PI3K/AKT-dependent component; 2) reduced acute stimulation of NHE3 activity by LPA/LPA5R stimulation; and 3) reduced acute inhibition of NHE3 activity-specifically, elevated Ca2+ related (carbachol/Ca2+ ionophore), but there was normal inhibition by forskolin and hyperosmolarity. Carbachol 316-325 solute carrier family 9 member A3 Homo sapiens 50-54 28406538-4 2017 Here, we find that the power, but not frequency, of optogenetically induced gamma oscillations in the CA3 region of mouse hippocampal slices is enhanced by low concentrations of the broad-spectrum cholinergic agonist carbachol but reduced at higher concentrations. Carbachol 217-226 carbonic anhydrase 3 Mus musculus 102-105 28332063-8 2017 Moreover, activation of muscarinic acetylcholine receptor (mAChR) by carbachol directly decreased the interaction between ZO-1 and occludin and increased the acinar TJ width in the freshly isolated human SMGs, whereas these effects were abolished by pretreatment with CK2 inhibitor. Carbachol 69-78 tight junction protein 1 Homo sapiens 122-126 28332063-8 2017 Moreover, activation of muscarinic acetylcholine receptor (mAChR) by carbachol directly decreased the interaction between ZO-1 and occludin and increased the acinar TJ width in the freshly isolated human SMGs, whereas these effects were abolished by pretreatment with CK2 inhibitor. Carbachol 69-78 occludin Homo sapiens 131-139 28496430-11 2017 Both nicotine and carbachol induced intracellular Ca2+ transients in trigeminal neurons partially overlapping with expression of capsaicin-sensitive TRPV1 receptors. Carbachol 18-27 transient receptor potential cation channel, subfamily V, member 1 Rattus norvegicus 149-154 28496430-1 2017 BACKGROUND: Parasympathetic innervation of meninges and ability of carbachol, acetylcholine (ACh) receptor (AChR) agonist, to induce headaches suggests contribution of cholinergic mechanisms to primary headaches. Carbachol 67-76 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 108-112 28223240-7 2017 Our results suggest that in the presence of carbachol both subiculum and CA3 most often drive theta generators in the entorhinal cortex and that these oscillations are influenced but not abolished by altering GABAA receptor signaling. Carbachol 44-53 carbonic anhydrase 3 Homo sapiens 73-76 28411158-9 2017 vGluT2+ neurons located in ventromedial regions of BF (in or adjacent to the horizontal limb of the diagonal band) were strongly hyperpolarized by the cholinergic agonist, carbachol, a finding apparently in conflict with their increased discharge during wakefulness/REM sleep and hypothesized role in wake-promotion. Carbachol 172-181 solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6 Mus musculus 0-6 27936472-8 2017 High KCl or carbachol-evoked elevation in the intracellular Ca2+ concentration was also abrogated by sodium tanshinone IIA sulphonate in tracheal smooth muscle cells. Carbachol 12-21 ATPase, class II, type 9A Mus musculus 119-122 28428632-6 2017 Carbachol (CCH) and forskolin (FSK) exerted significant effects on bladder ICC-LC [Ca2+]i in CYP-treated wild-type (WT) mice, and HCN1 channel ablation significantly decreased the effects of CCH and FSK on bladder ICC-LC [Ca2+]i in both naive and CYP-treated mice. Carbachol 11-14 hyperpolarization activated cyclic nucleotide gated potassium channel 1 Mus musculus 130-134 28428632-6 2017 Carbachol (CCH) and forskolin (FSK) exerted significant effects on bladder ICC-LC [Ca2+]i in CYP-treated wild-type (WT) mice, and HCN1 channel ablation significantly decreased the effects of CCH and FSK on bladder ICC-LC [Ca2+]i in both naive and CYP-treated mice. Carbachol 191-194 hyperpolarization activated cyclic nucleotide gated potassium channel 1 Mus musculus 130-134 27906750-3 2017 Our previous study showed that chemical stimulation of the lateral hypothalamus with carbachol induces antinociception in the tail-flick test, a model of acute pain, and Ox1r-mediated antinociception in the vlPAG is modulated by the activity of vlPAG CB1 receptors. Carbachol 85-94 hypocretin receptor 1 Rattus norvegicus 170-174 28539832-7 2017 We further demonstrated that vascular smooth muscle cells derived from FBN1-mutant iPSCs showed less sensitivity to carbachol as demonstrated by contractility and Ca2+ influx assay, compared to the isogenic controls cells. Carbachol 116-125 fibrillin 1 Homo sapiens 71-75 28284212-0 2017 Combination of glycopyrronium and indacaterol inhibits carbachol-induced ERK5 signal in fibrotic processes. Carbachol 55-64 mitogen-activated protein kinase 7 Homo sapiens 73-77 28284212-8 2017 Blockade of autocrine TGF-beta1 attenuated CCh-mediated fibrotic responses, while TGF-beta1 did not stimulate acetylcholine release. Carbachol 43-46 transforming growth factor beta 1 Homo sapiens 22-31 28284212-9 2017 Glycopyrronium plus indacaterol significantly attenuated CCh- and TGF-beta1-mediated fibrotic responses through inhibition of ERK5 phosphorylation. Carbachol 57-60 mitogen-activated protein kinase 7 Homo sapiens 126-130 28284212-10 2017 Notably, the magnitudes of CCh- and TGF-beta1-stimulated gel contraction, CCh-induced TGF-beta1 release, and ERK5 phosphorylation were greater in fibroblasts isolated from COPD subjects than in those from non-smokers. Carbachol 74-77 transforming growth factor beta 1 Homo sapiens 86-95 28284212-11 2017 CONCLUSIONS: CCh induced TGF-beta1 self-sustaining signaling loops by potentiating ERK5 signaling and promoted myofibroblast activity. Carbachol 13-16 transforming growth factor beta 1 Homo sapiens 25-34 28284212-11 2017 CONCLUSIONS: CCh induced TGF-beta1 self-sustaining signaling loops by potentiating ERK5 signaling and promoted myofibroblast activity. Carbachol 13-16 mitogen-activated protein kinase 7 Homo sapiens 83-87 28057557-8 2017 Whereas 100 muM chelerythrine (an inhibitor of PKC) and 100 muM carbachol (an activator of PLC) and 20 muM PD-98059 (an inhibitor of MEK) had additive effects on propofol-induced inhibition of ERK1/2 phosphorylation. Carbachol 64-73 mitogen-activated protein kinase 3 Mus musculus 193-199 27979597-5 2017 BTP2 inhibited carbachol-activated TRPC3 and TRPC6 channel activities in HEK293 cells. Carbachol 15-24 transient receptor potential cation channel subfamily C member 3 Homo sapiens 35-40 27979597-5 2017 BTP2 inhibited carbachol-activated TRPC3 and TRPC6 channel activities in HEK293 cells. Carbachol 15-24 transient receptor potential cation channel subfamily C member 6 Homo sapiens 45-50 28276525-5 2017 The muscarinic agonist carbachol, but not photic stimulation, phase-shifted Bmal1 transcriptional rhythms with a type-1 phase response curve. Carbachol 23-32 aryl hydrocarbon receptor nuclear translocator like Homo sapiens 76-81 28280417-7 2017 Furthermore, H2O2-induced elevation of intracellular Ca2+ levels was abolished under sarco/endoplasmic reticulum Ca2+ ATPase-inactivated condition by TG pretreatment with CCh. Carbachol 171-174 ATPase, Ca++ transporting, ubiquitous Mus musculus 85-124 27765842-6 2017 In isolated soleus muscle, clenbuterol, a selective beta2-adrenoceptor agonist, reduced the basal Ca2+-dependent proteolysis and completely abolished the activation of this pathway by the cholinergic agonist carbachol. Carbachol 208-217 adrenoceptor beta 2 Rattus norvegicus 52-70 28099849-3 2017 PTH alone had no effect on the cytosolic Ca2+ concentration, but it potentiated the Ca2+ signals evoked by carbachol. Carbachol 107-116 parathyroid hormone Homo sapiens 0-3 28099849-6 2017 We conclude that Ca2+ stores within the ER that dynamically exchange Ca2+ with the cytosol maintain a functional independence that allows one store to be released by carbachol and another to be released by carbachol with PTH. Carbachol 206-215 parathyroid hormone Homo sapiens 221-224 27906750-3 2017 Our previous study showed that chemical stimulation of the lateral hypothalamus with carbachol induces antinociception in the tail-flick test, a model of acute pain, and Ox1r-mediated antinociception in the vlPAG is modulated by the activity of vlPAG CB1 receptors. Carbachol 85-94 cannabinoid receptor 1 Rattus norvegicus 251-254 27773818-7 2016 We also observed that the inhibition of protein kinase C (PKC) by GF109203X greatly diminished carbachol-stimulated eIF4B phosphorylation. Carbachol 95-104 eukaryotic translation initiation factor 4B Homo sapiens 116-121 27606721-2 2017 Additional studies report the proliferative effect of the cholinergic agonist carbachol on prostate cancer by its agonistic action on CHRM3. Carbachol 78-87 cholinergic receptor muscarinic 3 Homo sapiens 134-139 27167250-4 2016 When the cells were treated with the cholinergic agonist carbachol, Akt was activated in a dose- and time-dependent fashion. Carbachol 57-66 AKT serine/threonine kinase 1 Homo sapiens 68-71 27167250-5 2016 This carbachol effect was almost completely blocked by the PI3K inhibitor LY294002, implying that PI3K is responsible for the Akt activation. Carbachol 5-14 AKT serine/threonine kinase 1 Homo sapiens 126-129 27167250-6 2016 S6K1, a major downstream target of mTORC1, was also activated by carbachol in a temporal profile similar to that of the Akt activation. Carbachol 65-74 ribosomal protein S6 kinase B1 Homo sapiens 0-4 27167250-6 2016 S6K1, a major downstream target of mTORC1, was also activated by carbachol in a temporal profile similar to that of the Akt activation. Carbachol 65-74 CREB regulated transcription coactivator 1 Mus musculus 35-41 27167250-7 2016 This carbachol-stimulated S6K1 activation was abrogated by LY294002 or the mTORC1 inhibitor rapamycin, supporting the notion that mAChRs mediate S6K1 activation via the PI3K-Akt-mTORC1 pathway. Carbachol 5-14 ribosomal protein S6 kinase B1 Homo sapiens 26-30 27167250-7 2016 This carbachol-stimulated S6K1 activation was abrogated by LY294002 or the mTORC1 inhibitor rapamycin, supporting the notion that mAChRs mediate S6K1 activation via the PI3K-Akt-mTORC1 pathway. Carbachol 5-14 CREB regulated transcription coactivator 1 Mus musculus 75-81 27167250-7 2016 This carbachol-stimulated S6K1 activation was abrogated by LY294002 or the mTORC1 inhibitor rapamycin, supporting the notion that mAChRs mediate S6K1 activation via the PI3K-Akt-mTORC1 pathway. Carbachol 5-14 ribosomal protein S6 kinase B1 Homo sapiens 145-149 27167250-7 2016 This carbachol-stimulated S6K1 activation was abrogated by LY294002 or the mTORC1 inhibitor rapamycin, supporting the notion that mAChRs mediate S6K1 activation via the PI3K-Akt-mTORC1 pathway. Carbachol 5-14 AKT serine/threonine kinase 1 Homo sapiens 174-177 27167250-7 2016 This carbachol-stimulated S6K1 activation was abrogated by LY294002 or the mTORC1 inhibitor rapamycin, supporting the notion that mAChRs mediate S6K1 activation via the PI3K-Akt-mTORC1 pathway. Carbachol 5-14 CREB regulated transcription coactivator 1 Mus musculus 178-184 27167250-9 2016 Inhibition experiments indicated that the ERK1/2 and mTORC1 signaling pathways may be involved in carbachol-stimulated global protein biosynthesis. Carbachol 98-107 mitogen-activated protein kinase 3 Homo sapiens 42-48 27167250-9 2016 Inhibition experiments indicated that the ERK1/2 and mTORC1 signaling pathways may be involved in carbachol-stimulated global protein biosynthesis. Carbachol 98-107 CREB regulated transcription coactivator 1 Mus musculus 53-59 27803431-3 2016 Recently, carbachol was reported to stabilize the wild-type hERG-FLAG via activation of the muscarinic type 3 receptor (M3-mAChR). Carbachol 10-19 ETS transcription factor ERG Homo sapiens 60-64 27803431-6 2016 Carbachol restored stability of the mutant hERG-FLAG and facilitated cell-surface expression. Carbachol 0-9 ETS transcription factor ERG Homo sapiens 43-47 27803431-7 2016 Carbachol activated PKC, augmented phosphorylation of heat shock factor 1 (HSF1) and enhanced expression of heat shock proteins (hsps), hsp70 and hsp90. Carbachol 0-9 heat shock transcription factor 1 Homo sapiens 54-73 27803431-7 2016 Carbachol activated PKC, augmented phosphorylation of heat shock factor 1 (HSF1) and enhanced expression of heat shock proteins (hsps), hsp70 and hsp90. Carbachol 0-9 heat shock transcription factor 1 Homo sapiens 75-79 27803431-7 2016 Carbachol activated PKC, augmented phosphorylation of heat shock factor 1 (HSF1) and enhanced expression of heat shock proteins (hsps), hsp70 and hsp90. Carbachol 0-9 heat shock protein family A (Hsp70) member 4 Homo sapiens 136-141 27803431-7 2016 Carbachol activated PKC, augmented phosphorylation of heat shock factor 1 (HSF1) and enhanced expression of heat shock proteins (hsps), hsp70 and hsp90. Carbachol 0-9 heat shock protein 90 alpha family class A member 1 Homo sapiens 146-151 27803431-8 2016 Both a M3-mAChR antagonist, 4-DAMP, and a PKC inhibitor, bisindolylmaleimide, abolished carbachol-induced stabilization of the mutant hERG-FLAG. Carbachol 88-97 ETS transcription factor ERG Homo sapiens 134-138 27287524-6 2016 RESULTS: Cholinergic stimulation of the detrusor induced by electrical field stimulation or exogenous application of carbachol or neostigmine evoked contractions consisting of a transient plus a tonic response, which was blocked by ML204, an inhibitor of TRPC4 channels. Carbachol 117-126 transient receptor potential cation channel, subfamily C, member 4 Mus musculus 255-260 27983984-4 2016 We further showed that the AngII-induced cells showed significant contractile responses to carbachol. Carbachol 91-100 angiotensinogen (serpin peptidase inhibitor, clade A, member 8) Mus musculus 27-32 27773818-3 2016 When SNU-407 cells were treated with the cholinergic agonist carbachol, eIF4B was phosphorylated in a dose- and time-dependent manner. Carbachol 61-70 eukaryotic translation initiation factor 4B Homo sapiens 72-77 27773818-4 2016 This carbachol effect was almost completely blocked by the muscarinic antagonist atropine, demonstrating that mAChRs specifically mediate the phosphorylation of eIF4B. Carbachol 5-14 eukaryotic translation initiation factor 4B Homo sapiens 161-166 27773818-5 2016 Carbachol-stimulated eIF4B phosphorylation was significantly reduced by the MEK1/2 inhibitor U0126, indicating that the MEK1/2-ERK1/2 pathway plays an important role in mAChR-mediated eIF4B phosphorylation. Carbachol 0-9 eukaryotic translation initiation factor 4B Homo sapiens 21-26 27773818-5 2016 Carbachol-stimulated eIF4B phosphorylation was significantly reduced by the MEK1/2 inhibitor U0126, indicating that the MEK1/2-ERK1/2 pathway plays an important role in mAChR-mediated eIF4B phosphorylation. Carbachol 0-9 mitogen-activated protein kinase kinase 1 Homo sapiens 76-82 27773818-5 2016 Carbachol-stimulated eIF4B phosphorylation was significantly reduced by the MEK1/2 inhibitor U0126, indicating that the MEK1/2-ERK1/2 pathway plays an important role in mAChR-mediated eIF4B phosphorylation. Carbachol 0-9 mitogen-activated protein kinase kinase 1 Homo sapiens 120-126 27773818-5 2016 Carbachol-stimulated eIF4B phosphorylation was significantly reduced by the MEK1/2 inhibitor U0126, indicating that the MEK1/2-ERK1/2 pathway plays an important role in mAChR-mediated eIF4B phosphorylation. Carbachol 0-9 mitogen-activated protein kinase 3 Homo sapiens 127-133 27773818-5 2016 Carbachol-stimulated eIF4B phosphorylation was significantly reduced by the MEK1/2 inhibitor U0126, indicating that the MEK1/2-ERK1/2 pathway plays an important role in mAChR-mediated eIF4B phosphorylation. Carbachol 0-9 eukaryotic translation initiation factor 4B Homo sapiens 184-189 27625606-11 2016 CYN154806, the SST2 receptor antagonist, dose-dependently and significantly reversed the decreased acid response to CCh in M4 but not M3 KO mice. Carbachol 116-119 somatostatin receptor 2 Mus musculus 15-19 27842583-9 2016 Results of urodynamic record of the TRPV1-/- mice during NS infusion showed reduced bladder pressure and contraction which exhibited decreased response to alpha, beta-me ATP, KCl, and carbachol and no response to CAP. Carbachol 184-193 transient receptor potential cation channel, subfamily V, member 1 Mus musculus 36-41 27543846-7 2016 However, catalase significantly reversed the DSS-induced reduction in baseline ion transport as well as colonic Isc responses to CCh. Carbachol 129-132 catalase Mus musculus 9-17 27301294-7 2016 CONCLUSION: It seems that antinociceptive effect of intra-LH administration of carbachol is mediated, at least partially, through the activation of orexin-1 and CB1 receptors in the vlPAG. Carbachol 79-88 cannabinoid receptor 1 Rattus norvegicus 161-164 27301294-11 2016 CB1 receptor antagonist (AM251) microinjection in the vlPAG prevented carbachol-induced antinociception in a dose-dependent manner. Carbachol 70-79 cannabinoid receptor 1 Rattus norvegicus 0-3 26895748-3 2016 In the present study, to examine the cholinergic signaling pathways responsible for the induction of a memory-related postsynaptic protein, a cholinergic agonist carbachol was used to induce the expression of activity-regulated cytoskeleton associated protein (Arc) in primary rat cortical neurons. Carbachol 162-171 activity-regulated cytoskeleton-associated protein Rattus norvegicus 209-259 26969473-5 2016 CA inhibition suppressed the forskolin-induced decrease in pHi, while it allowed carbachol to consistently increase pHi by revealing that carbachol prominently activated NHE via Ca2+-calmodulin. Carbachol 81-90 glucose-6-phosphate isomerase Rattus norvegicus 116-119 26969473-5 2016 CA inhibition suppressed the forskolin-induced decrease in pHi, while it allowed carbachol to consistently increase pHi by revealing that carbachol prominently activated NHE via Ca2+-calmodulin. Carbachol 138-147 glucose-6-phosphate isomerase Rattus norvegicus 116-119 26969473-6 2016 Under NHE inhibition, forskolin and carbachol induced the remarkable decreases in pHi, which were slowed predominantly by CA inhibition and by CA or anion channel inhibition, respectively. Carbachol 36-45 glucose-6-phosphate isomerase Rattus norvegicus 82-85 26969473-7 2016 Our results suggest that forskolin and carbachol primarily activate the pHi-lowering CA and pHi-raising NHE, respectively, to regulate pHi for HCO3- secretion. Carbachol 39-48 glucose-6-phosphate isomerase Rattus norvegicus 72-75 26969473-7 2016 Our results suggest that forskolin and carbachol primarily activate the pHi-lowering CA and pHi-raising NHE, respectively, to regulate pHi for HCO3- secretion. Carbachol 39-48 glucose-6-phosphate isomerase Rattus norvegicus 92-95 26969473-7 2016 Our results suggest that forskolin and carbachol primarily activate the pHi-lowering CA and pHi-raising NHE, respectively, to regulate pHi for HCO3- secretion. Carbachol 39-48 glucose-6-phosphate isomerase Rattus norvegicus 92-95 27550942-12 2016 Increased carbachol-induced chloride secretion was seen in irinotecan-treated wild-type and Tlr4-/- mice at 24 hours (wild-type: 100.35 +- 18.37 muA/cm2; P = 0.022; Tlr4-/-: 102.72 +- 18.80 muA/cm2; P = 0.023). Carbachol 10-19 toll-like receptor 4 Mus musculus 92-96 27550942-12 2016 Increased carbachol-induced chloride secretion was seen in irinotecan-treated wild-type and Tlr4-/- mice at 24 hours (wild-type: 100.35 +- 18.37 muA/cm2; P = 0.022; Tlr4-/-: 102.72 +- 18.80 muA/cm2; P = 0.023). Carbachol 10-19 toll-like receptor 4 Mus musculus 165-169 27707967-8 2016 The necessity of neuronal dystroglycan for functional innervation by CCK-positive basket cell axon terminals was confirmed by reduced frequency of inhibitory events in pyramidal cells of dystroglycan-deficient mice and further corroborated by the inefficiency of carbachol to increase IPSC frequency in these cells. Carbachol 263-272 cholecystokinin Mus musculus 69-72 27567078-5 2016 Here we report that previously described ligand LM11A-24 shows significant inhibition of carbachol-induced persistent firing (PF) of entorhinal cortex (EC) pyramidal neurons in wild-type mice via selective interaction with p75(NTR). Carbachol 89-98 nerve growth factor receptor (TNFR superfamily, member 16) Mus musculus 223-226 27567078-5 2016 Here we report that previously described ligand LM11A-24 shows significant inhibition of carbachol-induced persistent firing (PF) of entorhinal cortex (EC) pyramidal neurons in wild-type mice via selective interaction with p75(NTR). Carbachol 89-98 nerve growth factor receptor (TNFR superfamily, member 16) Mus musculus 227-230 27474091-3 2016 EXPERIMENTAL APPROACH: The effects of the ACh receptor agonist carbachol (CCh) on IFN-beta-induced apoptosis of human SH-SY5Y neuroblastoma cells were examined by using western blots, immunofluorescence and cytofluorimetry. Carbachol 63-72 interferon beta 1 Homo sapiens 82-90 27474091-3 2016 EXPERIMENTAL APPROACH: The effects of the ACh receptor agonist carbachol (CCh) on IFN-beta-induced apoptosis of human SH-SY5Y neuroblastoma cells were examined by using western blots, immunofluorescence and cytofluorimetry. Carbachol 74-77 interferon beta 1 Homo sapiens 82-90 27474091-7 2016 KEY RESULTS: In SH-SY5Y cells, CCh inhibited IFN-beta-induced mitochondrial cytochrome c release, activation of caspases 9, 7 and 3, PARP cleavage and DNA fragmentation. Carbachol 31-34 interferon beta 1 Homo sapiens 45-53 27474091-7 2016 KEY RESULTS: In SH-SY5Y cells, CCh inhibited IFN-beta-induced mitochondrial cytochrome c release, activation of caspases 9, 7 and 3, PARP cleavage and DNA fragmentation. Carbachol 31-34 cytochrome c, somatic Homo sapiens 76-88 27474091-7 2016 KEY RESULTS: In SH-SY5Y cells, CCh inhibited IFN-beta-induced mitochondrial cytochrome c release, activation of caspases 9, 7 and 3, PARP cleavage and DNA fragmentation. Carbachol 31-34 caspase 9 Homo sapiens 112-131 27474091-7 2016 KEY RESULTS: In SH-SY5Y cells, CCh inhibited IFN-beta-induced mitochondrial cytochrome c release, activation of caspases 9, 7 and 3, PARP cleavage and DNA fragmentation. Carbachol 31-34 collagen type XI alpha 2 chain Homo sapiens 133-137 27474091-8 2016 The anti-apoptotic effect of CCh was mediated by M3 receptors, blocked by Gq/11 antagonist YM254890 and PKC inhibitor Go 6983, impaired by inhibition of ERK1/2 pathway, potentiated by overexpression of ERK2 and mimicked by ERK2-CA. Carbachol 29-32 mitogen-activated protein kinase 3 Homo sapiens 153-159 27474091-8 2016 The anti-apoptotic effect of CCh was mediated by M3 receptors, blocked by Gq/11 antagonist YM254890 and PKC inhibitor Go 6983, impaired by inhibition of ERK1/2 pathway, potentiated by overexpression of ERK2 and mimicked by ERK2-CA. Carbachol 29-32 mitogen-activated protein kinase 1 Homo sapiens 202-206 27474091-8 2016 The anti-apoptotic effect of CCh was mediated by M3 receptors, blocked by Gq/11 antagonist YM254890 and PKC inhibitor Go 6983, impaired by inhibition of ERK1/2 pathway, potentiated by overexpression of ERK2 and mimicked by ERK2-CA. Carbachol 29-32 mitogen-activated protein kinase 1 Homo sapiens 223-227 27352269-8 2016 KEY RESULTS: Soluble inhibitors or PI3Kdelta knockdown reversed carbachol-induced constriction of human airways, relaxed agonist-contracted HASM and inhibited pAkt, pMYPT1 and pMLC in HASM. Carbachol 64-73 phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta Homo sapiens 35-44 27830759-5 2016 MCCV was increased by the cAMP-elevating agonists, forskolin or isoproterenol (10 muM) and by the Ca2+-elevating agonist, carbachol (0.3 muM). Carbachol 122-131 latexin Homo sapiens 137-140 27830759-8 2016 MCC velocity was most dramatically accelerated by the synergistic combination of forskolin and carbachol, which produced near-maximal clearance rates regardless of prior treatment with CFTR or ENaC inhibitors. Carbachol 95-104 CF transmembrane conductance regulator Homo sapiens 185-189 27474091-9 2016 Blockade of JNK activation enhanced the CCh anti-apoptotic response. Carbachol 40-43 mitogen-activated protein kinase 8 Homo sapiens 12-15 27474091-10 2016 IFN-beta inhibited JNK activation and up-regulated CCh-induced ERK1/2 signalling. Carbachol 51-54 interferon beta 1 Homo sapiens 0-8 27474091-10 2016 IFN-beta inhibited JNK activation and up-regulated CCh-induced ERK1/2 signalling. Carbachol 51-54 mitogen-activated protein kinase 3 Homo sapiens 63-69 27625606-12 2016 Octreotide, the somatostatin analog, inhibited the secretion of acid under CCh-stimulated conditions in WT mice. Carbachol 75-78 somatostatin Mus musculus 16-28 27509878-7 2016 In the functional studies, the urothelium removal from human bladder strips leads to an increase in carbachol-induced contraction that is mimicked by CBS inhibition. Carbachol 100-109 cystathionine beta-synthase Homo sapiens 150-153 27509878-9 2016 The increase in H2S production and in turn of cGMP is driven by CBS-cGMP/PKG-dependent phosphorylation at Ser(227) following carbachol stimulation. Carbachol 125-134 cystathionine beta-synthase Homo sapiens 64-67 27509878-9 2016 The increase in H2S production and in turn of cGMP is driven by CBS-cGMP/PKG-dependent phosphorylation at Ser(227) following carbachol stimulation. Carbachol 125-134 protein kinase cGMP-dependent 1 Homo sapiens 73-76 26909644-7 2016 P20 peptide caused dose-dependent relaxation of carbachol-precontracted ASM and blocked carbachol-induced contraction. Carbachol 48-57 tubulin polymerization promoting protein family member 3 Homo sapiens 0-3 26909644-7 2016 P20 peptide caused dose-dependent relaxation of carbachol-precontracted ASM and blocked carbachol-induced contraction. Carbachol 88-97 tubulin polymerization promoting protein family member 3 Homo sapiens 0-3 26871404-0 2016 Role of orexin-2 receptors in the nucleus accumbens in antinociception induced by carbachol stimulation of the lateral hypothalamus in formalin test. Carbachol 82-91 hypocretin neuropeptide precursor Homo sapiens 8-14 26871404-7 2016 The findings showed that TCS OX2 29 administration dose dependently blocked carbachol-induced antinociception during both phases of formalin-induced pain. Carbachol 76-85 CD200 molecule Homo sapiens 29-32 27320188-9 2016 H2O2 -induced dilatation, which occurred only at concentrations >=100microM, was reduced by a blocking antibody to TRPM2, which had no effect on carbachol-induced responses. Carbachol 148-157 transient receptor potential cation channel, subfamily M, member 2 Rattus norvegicus 118-123 27283411-11 2016 In HEK293T cells coexpressing TREK-2 and type 3 muscarinic receptor, application of carbachol induced transient activation and sustained suppression of ITREK-2,w-c and cell-attached ITREK-2. Carbachol 84-93 potassium channel, subfamily K, member 10 Mus musculus 30-36 27118568-0 2016 Carbachol-induced signaling through Thr696-phosphorylation of myosin phosphatase-targeting subunit 1 (MYPT1) in rat bladder smooth muscle cells. Carbachol 0-9 protein phosphatase 1, regulatory subunit 12A Rattus norvegicus 62-100 27118568-0 2016 Carbachol-induced signaling through Thr696-phosphorylation of myosin phosphatase-targeting subunit 1 (MYPT1) in rat bladder smooth muscle cells. Carbachol 0-9 protein phosphatase 1, regulatory subunit 12A Rattus norvegicus 102-107 27118568-3 2016 We attempt to directly observe the quantitative protein expression of Rho A/ROCK and phosphorylation of MYPT1 at Thr696 after carbachol administration in rat bladder smooth muscle cells (RBMSCs). Carbachol 126-135 ras homolog family member A Rattus norvegicus 70-75 27118568-3 2016 We attempt to directly observe the quantitative protein expression of Rho A/ROCK and phosphorylation of MYPT1 at Thr696 after carbachol administration in rat bladder smooth muscle cells (RBMSCs). Carbachol 126-135 protein phosphatase 1, regulatory subunit 12A Rattus norvegicus 104-109 27118568-5 2016 The effects of both concentration and time-course induced by the muscarinic agonist carbachol were investigated by assessing the expression of Rho A/ROCK and MYPT1 phosphorylation at Thr696 using Western blot. Carbachol 84-93 ras homolog family member A Rattus norvegicus 143-148 27118568-5 2016 The effects of both concentration and time-course induced by the muscarinic agonist carbachol were investigated by assessing the expression of Rho A/ROCK and MYPT1 phosphorylation at Thr696 using Western blot. Carbachol 84-93 protein phosphatase 1, regulatory subunit 12A Rattus norvegicus 158-163 27118568-6 2016 RESULTS: In the dose-course studies, carbachol showed significant increase in phosphorylation of MYPT1 at Thr696 (p-MYPT1) from concentrations of 15-100 muM based on Western blot results (p < 0.05, ANOVA test). Carbachol 37-46 protein phosphatase 1, regulatory subunit 12A Rattus norvegicus 97-102 27118568-6 2016 RESULTS: In the dose-course studies, carbachol showed significant increase in phosphorylation of MYPT1 at Thr696 (p-MYPT1) from concentrations of 15-100 muM based on Western blot results (p < 0.05, ANOVA test). Carbachol 37-46 protein phosphatase 1, regulatory subunit 12A Rattus norvegicus 116-121 27118568-7 2016 In the time-course studies, treatment of cells with 15 muM of carbachol significantly enhanced the expression of p-MYPT1 from 3 to 15 h (p < 0.05, ANOVA test) and induced the expression of Rho A from 10 to 120 min (p < 0.05, ANOVA test). Carbachol 62-71 protein phosphatase 1, regulatory subunit 12A Rattus norvegicus 115-120 27118568-7 2016 In the time-course studies, treatment of cells with 15 muM of carbachol significantly enhanced the expression of p-MYPT1 from 3 to 15 h (p < 0.05, ANOVA test) and induced the expression of Rho A from 10 to 120 min (p < 0.05, ANOVA test). Carbachol 62-71 ras homolog family member A Rattus norvegicus 192-197 27118568-8 2016 CONCLUSIONS: Carbachol can induce the expression of ROCK pathway, leading to MYPT1 phosphorylation at Thr696 and thereby sustained RBSMCs contraction. Carbachol 13-22 protein phosphatase 1, regulatory subunit 12A Rattus norvegicus 77-82 27084847-5 2016 TLR7 agonists were added to guinea pig airways following precontraction with carbachol in vitro or histamine in vivo. Carbachol 77-86 toll-like receptor 7 Cavia porcellus 0-4 27226533-9 2016 The muscarinic agonist carbachol induced pronounced transient PKCbetaI translocation and sustained recruitment of PKCepsilon. Carbachol 23-32 protein kinase C, epsilon Mus musculus 114-124 27226533-10 2016 When rise of [Ca(2+)]pm was prevented, the carbachol-induced DAG and PKCepsilon responses were somewhat reduced, but PKCbetaI translocation was completely abolished. Carbachol 43-52 protein kinase C, epsilon Mus musculus 69-79 26847850-2 2016 MYPT1 showed significant constitutive T696 and T853 phosphorylation, which is predicted to inhibit MLCP activity in isolated ileal smooth muscle tissues, with additional phosphorylation upon pharmacological treatment with the muscarinic agonist carbachol. Carbachol 245-254 protein phosphatase 1, regulatory subunit 12A Mus musculus 0-5 26847850-8 2016 Isolated MYPT1-deficient tissues from MYPT1(SM-/-) mice contracted and relaxed rapidly with moderate differences in sustained responses to KCl and carbachol treatments and washouts, respectively. Carbachol 147-156 protein phosphatase 1, regulatory subunit 12A Mus musculus 9-14 26752781-3 2016 The anti-fibrillatory action of carbamylcholine was prevented by the nicotinic receptor antagonist mecamylamine, inhibitors of neuronal nitric oxide synthase (nNOS) and soluble guanylyl cyclase (sGC), and can be mimicked by the nitric oxide (NO) donor sodium nitroprusside. Carbachol 32-47 nitric oxide synthase 1 Homo sapiens 127-157 26752781-3 2016 The anti-fibrillatory action of carbamylcholine was prevented by the nicotinic receptor antagonist mecamylamine, inhibitors of neuronal nitric oxide synthase (nNOS) and soluble guanylyl cyclase (sGC), and can be mimicked by the nitric oxide (NO) donor sodium nitroprusside. Carbachol 32-47 nitric oxide synthase 1 Homo sapiens 159-163 26752781-6 2016 These data demonstrate a protective effect of carbamylcholine on VFT that depends upon both muscarinic and nicotinic receptor stimulation, where the generation of NO is likely to be via a neuronal nNOS-sGC dependent pathway. Carbachol 46-61 nitric oxide synthase 1 Homo sapiens 197-201 26752781-17 2016 These data demonstrate a protective effect of CCh on VFT that depends upon both muscarinic and nicotinic receptor stimulation, where the generation of NO is likely to be via a neuronal nNOS/sGC-dependent pathway. Carbachol 46-49 nitric oxide synthase 1 Homo sapiens 185-189 27282572-4 2016 Adding carbachol which is a cholinergic agonist to the ATRA treatment resulted in an increase of a granulocytic differentiation marker (CD11b) as compared with ATRA treatment alone (p<0.05), indicating that cholinergic activation enhanced ATRA in inducing NB-4 maturation. Carbachol 7-16 integrin subunit alpha M Homo sapiens 136-141 26966862-9 2016 The initial rates of CCh-stimulated cell volume reduction in acinar cells from hAQP1-expressing glands post IR were similar to those from control cells. Carbachol 21-24 aquaporin 1 (Colton blood group) Homo sapiens 79-84 27076615-7 2016 The reliance of CTSS secretion on Rab27 activity was supported by in vitro findings that newly synthesized CTSS was detected in and secreted from Rab27-enriched secretory vesicles and that expression of dominant negative Rab27b reduced carbachol-stimulated secretion of CTSS in cultured LGAC. Carbachol 236-245 cathepsin S Mus musculus 16-20 27076615-7 2016 The reliance of CTSS secretion on Rab27 activity was supported by in vitro findings that newly synthesized CTSS was detected in and secreted from Rab27-enriched secretory vesicles and that expression of dominant negative Rab27b reduced carbachol-stimulated secretion of CTSS in cultured LGAC. Carbachol 236-245 RAB27A, member RAS oncogene family Homo sapiens 34-39 27076615-7 2016 The reliance of CTSS secretion on Rab27 activity was supported by in vitro findings that newly synthesized CTSS was detected in and secreted from Rab27-enriched secretory vesicles and that expression of dominant negative Rab27b reduced carbachol-stimulated secretion of CTSS in cultured LGAC. Carbachol 236-245 cathepsin S Mus musculus 107-111 27076615-7 2016 The reliance of CTSS secretion on Rab27 activity was supported by in vitro findings that newly synthesized CTSS was detected in and secreted from Rab27-enriched secretory vesicles and that expression of dominant negative Rab27b reduced carbachol-stimulated secretion of CTSS in cultured LGAC. Carbachol 236-245 RAB27B, member RAS oncogene family Mus musculus 221-227 27076615-7 2016 The reliance of CTSS secretion on Rab27 activity was supported by in vitro findings that newly synthesized CTSS was detected in and secreted from Rab27-enriched secretory vesicles and that expression of dominant negative Rab27b reduced carbachol-stimulated secretion of CTSS in cultured LGAC. Carbachol 236-245 cathepsin S Mus musculus 107-111 26879866-9 2016 Plasma vasopressin levels evaluated by ELISA kit assay increased after carbachol into the 4th V. Intravenous blockade of V1 receptors prior to carbachol into the 4thV abolished the increase in IP evoked by carbachol. Carbachol 71-80 arginine vasopressin Rattus norvegicus 7-18 26911851-7 2016 The stimulatory effect of 8MM-IBMX on TBKCs was reversed upon activation of muscarinic acetylcholine receptors with carbachol (1 muM). Carbachol 116-125 latexin Homo sapiens 129-132 26879866-9 2016 Plasma vasopressin levels evaluated by ELISA kit assay increased after carbachol into the 4th V. Intravenous blockade of V1 receptors prior to carbachol into the 4thV abolished the increase in IP evoked by carbachol. Carbachol 143-152 arginine vasopressin Rattus norvegicus 7-18 26879866-9 2016 Plasma vasopressin levels evaluated by ELISA kit assay increased after carbachol into the 4th V. Intravenous blockade of V1 receptors prior to carbachol into the 4thV abolished the increase in IP evoked by carbachol. Carbachol 143-152 arginine vasopressin Rattus norvegicus 7-18 26879866-10 2016 Therefore, our findings suggest that cholinergic activation of neurons in the medulla oblongata by carbachol injections into the 4thV increases IP due to plasma vasopressin release, which acts in V1 receptors in the UB. Carbachol 99-108 arginine vasopressin Rattus norvegicus 161-172 26661936-10 2016 The GPER agonist G-1 caused a concentration-dependent inhibition of carbachol -induced circular muscle strips contraction, which was abolished by tetrodotoxin and the neuronal nitric oxide synthase (nNOS) inhibitor N-propyl-l-arginine. Carbachol 68-77 G protein-coupled estrogen receptor 1 Mus musculus 4-8 26961238-3 2016 Loss of Rgs5 provoked dramatically exaggerated bradycardia and significantly (P<0.05) prolonged sinus nodal recovery time in response to carbachol (0.1 mg/kg, intraperitoneally). Carbachol 140-149 regulator of G-protein signaling 5 Mus musculus 8-12 26956674-11 2016 Carbachol-induced ASM cell migration was reduced by selective inhibitors of PI3K/Akt (LY294002) and p38 (SB203580), suggesting that it occurred through p38 and Akt phosphorylation, which was inhibited by the M3 mAChR antagonist 4-DAMP. Carbachol 0-9 AKT serine/threonine kinase 1 Homo sapiens 81-84 26956674-11 2016 Carbachol-induced ASM cell migration was reduced by selective inhibitors of PI3K/Akt (LY294002) and p38 (SB203580), suggesting that it occurred through p38 and Akt phosphorylation, which was inhibited by the M3 mAChR antagonist 4-DAMP. Carbachol 0-9 mitogen-activated protein kinase 14 Homo sapiens 100-103 26956674-11 2016 Carbachol-induced ASM cell migration was reduced by selective inhibitors of PI3K/Akt (LY294002) and p38 (SB203580), suggesting that it occurred through p38 and Akt phosphorylation, which was inhibited by the M3 mAChR antagonist 4-DAMP. Carbachol 0-9 mitogen-activated protein kinase 14 Homo sapiens 152-155 26956674-11 2016 Carbachol-induced ASM cell migration was reduced by selective inhibitors of PI3K/Akt (LY294002) and p38 (SB203580), suggesting that it occurred through p38 and Akt phosphorylation, which was inhibited by the M3 mAChR antagonist 4-DAMP. Carbachol 0-9 AKT serine/threonine kinase 1 Homo sapiens 160-163 25523105-4 2016 Microglia cultured from adult and neonatal brain contained a carbachol-sensitive subpopulation (8 and 9 %), which was increased by treatment with interferon-gamma to around 60 %. Carbachol 61-70 interferon gamma Mus musculus 146-162 26660312-5 2016 When TRPP2 protein was knocked down in gallbladder muscle strips from guinea pig, carbachol (CCh)-evoked Ca(2+) release and extracellular Ca(2+) influx were reduced significantly, and gallbladder contractions induced by endothelin 1 and cholecystokinin were suppressed markedly as well. Carbachol 93-96 endothelin-1 Cavia porcellus 220-232 26661936-10 2016 The GPER agonist G-1 caused a concentration-dependent inhibition of carbachol -induced circular muscle strips contraction, which was abolished by tetrodotoxin and the neuronal nitric oxide synthase (nNOS) inhibitor N-propyl-l-arginine. Carbachol 68-77 nitric oxide synthase 1, neuronal Mus musculus 167-197 26661936-10 2016 The GPER agonist G-1 caused a concentration-dependent inhibition of carbachol -induced circular muscle strips contraction, which was abolished by tetrodotoxin and the neuronal nitric oxide synthase (nNOS) inhibitor N-propyl-l-arginine. Carbachol 68-77 nitric oxide synthase 1, neuronal Mus musculus 199-203 27555117-4 2016 The bradycardia following the microinjection of CCh into the PHN can be attenuated by the previous administration of the vasopressin V1 receptor antagonist [d(CH2 )5 Tyr(Me)] arginine vasopressin (AVPX). Carbachol 48-51 arginine vasopressin Rattus norvegicus 121-132 26656315-6 2016 KEY FINDINGS: Our study observed the protective effect of carbachol postconditioning on H/R-induced injury in human gastric epithelial cell lines (hGES-1) cells, which is achieved by direct activation of vanilloid receptor subtype 1 (VR1) and production of calcitonin gene-related peptide (CGRP), and in the inhibition of cell apoptosis. Carbachol 58-67 transient receptor potential cation channel subfamily V member 1 Homo sapiens 204-232 26656315-6 2016 KEY FINDINGS: Our study observed the protective effect of carbachol postconditioning on H/R-induced injury in human gastric epithelial cell lines (hGES-1) cells, which is achieved by direct activation of vanilloid receptor subtype 1 (VR1) and production of calcitonin gene-related peptide (CGRP), and in the inhibition of cell apoptosis. Carbachol 58-67 transient receptor potential cation channel subfamily V member 1 Homo sapiens 234-237 26656315-6 2016 KEY FINDINGS: Our study observed the protective effect of carbachol postconditioning on H/R-induced injury in human gastric epithelial cell lines (hGES-1) cells, which is achieved by direct activation of vanilloid receptor subtype 1 (VR1) and production of calcitonin gene-related peptide (CGRP), and in the inhibition of cell apoptosis. Carbachol 58-67 calcitonin related polypeptide alpha Homo sapiens 257-288 26656315-6 2016 KEY FINDINGS: Our study observed the protective effect of carbachol postconditioning on H/R-induced injury in human gastric epithelial cell lines (hGES-1) cells, which is achieved by direct activation of vanilloid receptor subtype 1 (VR1) and production of calcitonin gene-related peptide (CGRP), and in the inhibition of cell apoptosis. Carbachol 58-67 calcitonin related polypeptide alpha Homo sapiens 290-294 25520285-4 2016 Functionally, the contractile embryoid bodies (EBs) displayed calcium cycling and were responsive to the chronotropic agents isoprenaline (0.1 muM) and carbachol (1 muM). Carbachol 152-161 latexin Homo sapiens 165-168 27555117-4 2016 The bradycardia following the microinjection of CCh into the PHN can be attenuated by the previous administration of the vasopressin V1 receptor antagonist [d(CH2 )5 Tyr(Me)] arginine vasopressin (AVPX). Carbachol 48-51 arginine vasopressin Rattus norvegicus 184-195 27555117-6 2016 The attenuation by AVPX of the bradycardia that results following the high doses of CCh suggests that AVP is released into the circulation following stimulation of cholinergic systems within the PHN. Carbachol 84-87 arginine vasopressin Rattus norvegicus 19-22 27555117-7 2016 Thus, microinjection of a high dose of CCh (11 nmol) into the PHN alters the sensitivity of the baroreceptor reflex by increasing peripheral levels of AVP. Carbachol 39-42 arginine vasopressin Rattus norvegicus 151-154 26606130-3 2016 AF710B exhibits an allosteric agonistic profile on the M1 muscarinic receptor; very low concentrations of AF710B significantly potentiated the binding and efficacy of carbachol on M1 receptors and their downstream effects (p-ERK1/2, p-CREB). Carbachol 167-176 mitogen activated protein kinase 3 Rattus norvegicus 225-231 26129651-6 2016 IFN-gamma itself increased [Ca(2+)](i) in rat and human goblet cells and prevented the increase in [Ca(2+)](i) caused by carbachol. Carbachol 121-130 interferon gamma Rattus norvegicus 0-9 26129651-7 2016 Carbachol prevented IFN-gamma-mediated increase in [Ca(2+)](i). Carbachol 0-9 interferon gamma Homo sapiens 20-29 26129651-9 2016 IFN-gamma blocked carbachol-induced high molecular weight glycoconjugate secretion and reduced goblet cell proliferation. Carbachol 18-27 interferon gamma Homo sapiens 0-9 26606130-3 2016 AF710B exhibits an allosteric agonistic profile on the M1 muscarinic receptor; very low concentrations of AF710B significantly potentiated the binding and efficacy of carbachol on M1 receptors and their downstream effects (p-ERK1/2, p-CREB). Carbachol 167-176 cAMP responsive element binding protein 1 Rattus norvegicus 235-239 25919006-7 2015 Individually, IL-17A and IL-25 enhanced contractility of human bronchial smooth muscle induced by methacholine or carbachol. Carbachol 114-123 interleukin 17A Homo sapiens 14-20 25919006-7 2015 Individually, IL-17A and IL-25 enhanced contractility of human bronchial smooth muscle induced by methacholine or carbachol. Carbachol 114-123 interleukin 25 Homo sapiens 25-30 26344105-5 2015 This carbachol-induced inhibitory effect on Ca(2+) oscillations in myocytes and pericytes was reversed by ODQ, an inhibitor of soluble guanylyl cyclase (sGC) and by Rp-8-pCPT-cGMPS, an inhibitor of protein kinase G (PKG). Carbachol 5-14 protein kinase cGMP-dependent 1 Homo sapiens 198-214 26344105-5 2015 This carbachol-induced inhibitory effect on Ca(2+) oscillations in myocytes and pericytes was reversed by ODQ, an inhibitor of soluble guanylyl cyclase (sGC) and by Rp-8-pCPT-cGMPS, an inhibitor of protein kinase G (PKG). Carbachol 5-14 protein kinase cGMP-dependent 1 Homo sapiens 216-219 26494513-9 2015 The findings of this study showed that the OX2 receptor has a critical role in modulating reward circuit in the VTA and NAc, when the LH was stimulated by carbachol. Carbachol 155-164 CD200 receptor 1 Rattus norvegicus 43-55 26546746-4 2015 TGF-beta1+carbachol enhanced the generation of mesenchymal cells, which was significantly reduced by aclidinium bromide or formoterol. Carbachol 10-19 transforming growth factor beta 1 Homo sapiens 0-9 26648844-6 2015 Inhibition of the principal eCB CB1 receptor blocked carbachol induced LTD in both rats and mice. Carbachol 53-62 cannabinoid receptor 1 Rattus norvegicus 32-35 26648844-7 2015 Furthermore, when challenged with a sub-threshold carbachol application, LTD was induced in slices pretreated with the monoacylglycerol lipase (MAGL) inhibitor JZL184, suggesting that the eCB 2-arachidonylglyerol (2-AG) mediates M1 mAChR LTD. Carbachol 50-59 monoglyceride lipase Rattus norvegicus 119-142 26648844-7 2015 Furthermore, when challenged with a sub-threshold carbachol application, LTD was induced in slices pretreated with the monoacylglycerol lipase (MAGL) inhibitor JZL184, suggesting that the eCB 2-arachidonylglyerol (2-AG) mediates M1 mAChR LTD. Carbachol 50-59 monoglyceride lipase Rattus norvegicus 144-148 26546746-7 2015 In mesenchymal cells, TGF-beta1+carbachol induced the deposition of collagen-I and fibronectin which was prevented by both drugs dose-dependently. Carbachol 32-41 fibronectin 1 Homo sapiens 83-94 26294392-8 2015 CCh and BK increased phosphorylation of MYPT-1 and MLC20 and auto-phosphorylation of SrcFK and FAK. Carbachol 0-3 protein phosphatase 1 regulatory subunit 12A Homo sapiens 40-46 26909310-6 2015 RESULTS: Deletion of ABHD6 potentiated insulin secretion in response to the fuels glutamine plus leucine and alpha-ketoisocaproate and to the non-fuel stimuli glucagon-like peptide 1, carbamylcholine and elevated KCl. Carbachol 184-199 abhydrolase domain containing 6 Mus musculus 21-26 25979836-5 2015 PAK2 was activated by some pancreatic growth-factors [EGF, PDGF, bFGF], by secretagogues activating phospholipase-C (PLC) [CCK, carbachol, bombesin] and by post-receptor stimulants activating PKC [TPA], but not agents only mobilizing cellular calcium or increasing cyclic AMP. Carbachol 128-137 p21 (RAC1) activated kinase 2 Rattus norvegicus 0-4 25812766-8 2015 In this paper, we investigated the ability of a combination of the cytotoxic drug paclitaxel plus carbachol, a cholinergic agonist, at low doses, to induce death in breast tumor MCF-7 cells, via mAChR activation, and the role of nitric oxide synthase (NOS) and arginase in this effect. Carbachol 98-107 nitric oxide synthase 1 Homo sapiens 229-250 26051129-6 2015 All subtypes of prostaglandin E2 (PGE2) receptors (EP1-EP4) were expressed in ileum, and PGE2 and selective EP2 or EP4 agonist inhibited CCh-mediated contraction. Carbachol 137-140 prostaglandin E receptor 1 Rattus norvegicus 51-58 26051129-6 2015 All subtypes of prostaglandin E2 (PGE2) receptors (EP1-EP4) were expressed in ileum, and PGE2 and selective EP2 or EP4 agonist inhibited CCh-mediated contraction. Carbachol 137-140 prostaglandin E receptor 2 Rattus norvegicus 108-111 26051129-6 2015 All subtypes of prostaglandin E2 (PGE2) receptors (EP1-EP4) were expressed in ileum, and PGE2 and selective EP2 or EP4 agonist inhibited CCh-mediated contraction. Carbachol 137-140 prostaglandin E receptor 4 Rattus norvegicus 55-58 26051129-9 2015 Finally, in ileal tissues isolated from peritonitis model rat, iNOS expression was upregulated only at 4 hr after LPS administration, resulting in enhanced inhibitory action of LPS against CCh-induced contraction. Carbachol 189-192 nitric oxide synthase 2 Rattus norvegicus 63-67 26051129-11 2015 Moreover, in late phase of LPS treatment, iNOS is expressed to produce NO, which in turn inhibited the contraction by CCh. Carbachol 118-121 nitric oxide synthase 2 Rattus norvegicus 42-46 26071486-7 2015 Overexpression of CHRM3 or activation of CHRM3 by carbachol promoted cell proliferation, migration, and castration resistance. Carbachol 50-59 cholinergic receptor muscarinic 3 Homo sapiens 41-46 26355753-4 2015 Addition of the full agonist carbamoylcholine activated and opened the nAChR ion channel, as revealed by the increase in capacitance relative to that of the nAChR-thiolipid system under basal conditions. Carbachol 29-45 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 71-76 26355753-4 2015 Addition of the full agonist carbamoylcholine activated and opened the nAChR ion channel, as revealed by the increase in capacitance relative to that of the nAChR-thiolipid system under basal conditions. Carbachol 29-45 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 157-162 26378782-9 2015 Histamine/carbachol stimulated gastric acid secretion was significantly reduced (range 84-95%, P<0.005) in ClC-2-/- compared to WT, while pepsinogen secretion was unaffected. Carbachol 10-19 chloride channel, voltage-sensitive 2 Mus musculus 110-115 26365984-6 2015 Similar decreases in IP3R and carbachol (CCh)-stimulated [Ca(2+)]i elevation were detected in acinar cells from lymphotoxin-alpha (LTalpha) transgenic (TG) mice, a model for (SS). Carbachol 30-39 lymphotoxin A Mus musculus 112-129 26365984-6 2015 Similar decreases in IP3R and carbachol (CCh)-stimulated [Ca(2+)]i elevation were detected in acinar cells from lymphotoxin-alpha (LTalpha) transgenic (TG) mice, a model for (SS). Carbachol 30-39 lymphotoxin A Mus musculus 131-138 26365984-6 2015 Similar decreases in IP3R and carbachol (CCh)-stimulated [Ca(2+)]i elevation were detected in acinar cells from lymphotoxin-alpha (LTalpha) transgenic (TG) mice, a model for (SS). Carbachol 41-44 lymphotoxin A Mus musculus 112-129 26365984-6 2015 Similar decreases in IP3R and carbachol (CCh)-stimulated [Ca(2+)]i elevation were detected in acinar cells from lymphotoxin-alpha (LTalpha) transgenic (TG) mice, a model for (SS). Carbachol 41-44 lymphotoxin A Mus musculus 131-138 26215661-9 2015 The effect of carbachol (100 muM) and isoproterenol (4 mug mL(-1)) on single cells and groups of cells was demonstrated and the feature for immunostaining (beta-actin) applicability of the chip was revealed. Carbachol 14-23 actin, beta Rattus norvegicus 156-166 26294392-8 2015 CCh and BK increased phosphorylation of MYPT-1 and MLC20 and auto-phosphorylation of SrcFK and FAK. Carbachol 0-3 myosin light chain 12B Homo sapiens 51-56 26294392-8 2015 CCh and BK increased phosphorylation of MYPT-1 and MLC20 and auto-phosphorylation of SrcFK and FAK. Carbachol 0-3 protein tyrosine kinase 2 Homo sapiens 95-98 26229434-7 2015 On the contrary, carbachol could enhance the phosphorylation level of Nedd4-2 as an alternative to SGK1, and thus rescue the ubiquitin-mediated degradation of hERG channels caused by probucol. Carbachol 17-26 NEDD4 like E3 ubiquitin protein ligase Homo sapiens 70-77 26358343-5 2015 RESULTS: Carbachol-stimulated secretion rates were the following: controls, 1670 +- 381 pl min(-1) gland(-1); CRS, 965 +- 440 pl min(-1) gland(-1); and CF, 933 +- 588 pl min(-1) gland(-1) (p = 0.23, Kruskal-Wallis test). Carbachol 9-18 CD59 molecule (CD59 blood group) Homo sapiens 91-97 26358343-5 2015 RESULTS: Carbachol-stimulated secretion rates were the following: controls, 1670 +- 381 pl min(-1) gland(-1); CRS, 965 +- 440 pl min(-1) gland(-1); and CF, 933 +- 588 pl min(-1) gland(-1) (p = 0.23, Kruskal-Wallis test). Carbachol 9-18 CD59 molecule (CD59 blood group) Homo sapiens 129-135 26358343-5 2015 RESULTS: Carbachol-stimulated secretion rates were the following: controls, 1670 +- 381 pl min(-1) gland(-1); CRS, 965 +- 440 pl min(-1) gland(-1); and CF, 933 +- 588 pl min(-1) gland(-1) (p = 0.23, Kruskal-Wallis test). Carbachol 9-18 CD59 molecule (CD59 blood group) Homo sapiens 129-135 26169369-8 2015 Moreover, the activating phosphorylation and Golgi translocation of endothelial NO synthase in response to the M3R agonist carbachol were diminished. Carbachol 123-132 cholinergic receptor, muscarinic 3, cardiac Mus musculus 111-114 26258553-8 2015 Pre-contracted telokin-/- gastric fundus smooth muscles have increased contractile responses to KCl, CCh, or cholinergic neurotransmission and reduced relaxation to 8-Bromo-cGMP, SNP, and nitrergic neurotransmission. Carbachol 101-104 myosin, light polypeptide kinase Mus musculus 15-22 26405813-6 2015 RESULTS: Treatment of small intestinal tissue with clotrimazole inhibited the Cl- secretory currents that resulted from challenge with the cAMP-agonist vasoactive intestinal peptide (VIP) or Ca(2+)-agonist carbachol in a dose-dependent fashion. Carbachol 206-215 vasoactive intestinal peptide Homo sapiens 183-186 25934671-11 2015 Nitrofen-treated lungs exhibited an increased number of proliferating Sox9-positive distal epithelial progenitor cells, which were decreased and normalized by treatment with carbachol. Carbachol 174-183 SRY-box transcription factor 9 Homo sapiens 70-74 26229434-7 2015 On the contrary, carbachol could enhance the phosphorylation level of Nedd4-2 as an alternative to SGK1, and thus rescue the ubiquitin-mediated degradation of hERG channels caused by probucol. Carbachol 17-26 ETS transcription factor ERG Homo sapiens 159-163 26021821-8 2015 In addition, the glucagon-mediated impairment of carbachol-induced contraction was prevented by either removing epithelial cells or blocking NOS (L-NAME), COX (indomethacin) or COX-1 (SC-560). Carbachol 49-58 cytochrome c oxidase I, mitochondrial Mus musculus 177-182 25956403-10 2015 In the presence of carbachol, both beta-AR agonists increased MLC phosphorylation, an effect reduced by Y27,632 only in the presence of 1 muM carbachol. Carbachol 19-28 modulator of VRAC current 1 Homo sapiens 62-65 25956403-10 2015 In the presence of carbachol, both beta-AR agonists increased MLC phosphorylation, an effect reduced by Y27,632 only in the presence of 1 muM carbachol. Carbachol 142-151 latexin Homo sapiens 138-141 25703905-4 2015 Functional studies in the presence of muscarinic receptor and nitric oxide synthase (NOS) antagonists revealed that in M3 receptor-mediated carbachol secretion, nitric oxide, deriving mainly from neuronal NOS (nNOS) in control, and iNOS in diabetic rabbits, was involved. Carbachol 140-149 nitric oxide synthase, brain Oryctolagus cuniculus 62-83 25703905-4 2015 Functional studies in the presence of muscarinic receptor and nitric oxide synthase (NOS) antagonists revealed that in M3 receptor-mediated carbachol secretion, nitric oxide, deriving mainly from neuronal NOS (nNOS) in control, and iNOS in diabetic rabbits, was involved. Carbachol 140-149 nitric oxide synthase, brain Oryctolagus cuniculus 196-208 25703905-4 2015 Functional studies in the presence of muscarinic receptor and nitric oxide synthase (NOS) antagonists revealed that in M3 receptor-mediated carbachol secretion, nitric oxide, deriving mainly from neuronal NOS (nNOS) in control, and iNOS in diabetic rabbits, was involved. Carbachol 140-149 nitric oxide synthase, brain Oryctolagus cuniculus 210-214 25703905-4 2015 Functional studies in the presence of muscarinic receptor and nitric oxide synthase (NOS) antagonists revealed that in M3 receptor-mediated carbachol secretion, nitric oxide, deriving mainly from neuronal NOS (nNOS) in control, and iNOS in diabetic rabbits, was involved. Carbachol 140-149 nitric oxide synthase, inducible Oryctolagus cuniculus 232-236 25139191-0 2015 Carbachol-induced colonic mucus formation requires transport via NKCC1, K+ channels and CFTR. Carbachol 0-9 solute carrier family 12, member 2 Mus musculus 65-70 25139191-0 2015 Carbachol-induced colonic mucus formation requires transport via NKCC1, K+ channels and CFTR. Carbachol 0-9 cystic fibrosis transmembrane conductance regulator Mus musculus 88-92 25139191-7 2015 The results showed that the carbachol-induced increase in membrane current was dependent on NKCC1 co-transport, basolateral K(+) channels and Cftr activity. Carbachol 28-37 solute carrier family 12, member 2 Mus musculus 92-97 25139191-7 2015 The results showed that the carbachol-induced increase in membrane current was dependent on NKCC1 co-transport, basolateral K(+) channels and Cftr activity. Carbachol 28-37 cystic fibrosis transmembrane conductance regulator Mus musculus 142-146 25139191-8 2015 In contrast, the carbachol-induced increase in capacitance was partially dependent on NKCC1 and K(+) channel activity, but did not require Cftr activity. Carbachol 17-26 solute carrier family 12, member 2 Mus musculus 86-91 25139191-9 2015 Carbachol also induced an increase in mucus thickness that was inhibited by the NKCC1 blocker bumetanide. Carbachol 0-9 solute carrier family 12, member 2 Mus musculus 80-85 25139191-10 2015 However, mice that lacked a functional Cftr channel did not respond to carbachol with an increase in mucus thickness, suggesting that carbachol-induced mucin expansion requires Cftr channel activity. Carbachol 134-143 cystic fibrosis transmembrane conductance regulator Mus musculus 39-43 25139191-10 2015 However, mice that lacked a functional Cftr channel did not respond to carbachol with an increase in mucus thickness, suggesting that carbachol-induced mucin expansion requires Cftr channel activity. Carbachol 134-143 cystic fibrosis transmembrane conductance regulator Mus musculus 177-181 25948584-4 2015 Carbachol induced downregulation and redistribution of claudin-4, but not occludin or ZO-1 (also known as TJP1). Carbachol 0-9 claudin 4 Rattus norvegicus 55-64 25948584-5 2015 Small hairpin RNA (shRNA)-mediated claudin-4 knockdown suppressed, whereas claudin-4 overexpression retained, the TER response to carbachol. Carbachol 130-139 claudin 4 Rattus norvegicus 75-84 25948584-7 2015 Mutagenesis assay demonstrated that S195, but not S199, S203 or S207, of claudin-4, was the target for carbachol. Carbachol 103-112 claudin 4 Rattus norvegicus 73-82 25788576-9 2015 In HEK293 cells transfected with the M2 muscarinic receptor, the application of carbachol increased the FRET efficiency between TRPC4beta-CFP and Galphai2(WT)-YFP from 4.7 +- 0.4% (n = 7) to 12.6 +- 1.4% (n = 7). Carbachol 80-89 complement factor properdin Homo sapiens 138-141 25788576-12 2015 In response to the muscarinic agonist carbachol, M2-, Galphai2-, and TRPC4-expressing cells showed a prolonged Ca(2+) influx compared with cells expressing only M2. Carbachol 38-47 transient receptor potential cation channel subfamily C member 4 Homo sapiens 69-74 26742661-8 2015 Since the same response is elicited at a lower level by CBL administration, the hypothesis of an involvement of cholinoceptive ORX neurons in its generation is discussed. Carbachol 56-59 hypocretin neuropeptide precursor Rattus norvegicus 127-130 25382267-0 2015 The Role of Rac1 on Carbachol-induced Contractile Activity in Detrusor Smooth Muscle from Streptozotocin-induced Diabetic Rats. Carbachol 20-29 Rac family small GTPase 1 Rattus norvegicus 12-16 25382267-1 2015 This study was designed to determine the role of the small GTPase Rac1 on carbachol-induced contractile activity in detrusor smooth muscle using small inhibitor NSC 23766 in diabetic rats. Carbachol 74-83 Rac family small GTPase 1 Rattus norvegicus 66-70 25382267-8 2015 Rac1 inhibitor NSC 23766 inhibited CCh-induced contractile responses in all groups, but this inhibition seen in both diabetes groups was greater than in the control group. Carbachol 35-38 Rac family small GTPase 1 Rattus norvegicus 0-4 25382267-11 2015 In the diabetic bladders, increased expression of Rac1 and considerable inhibition of CCh-induced responses in the presence of NSC 23766 compared to those of the control group may indicate a specific role of Rac1 in diabetes-related bladder dysfunction, especially associated with cholinergic mediated detrusor overactivity. Carbachol 86-89 Rac family small GTPase 1 Rattus norvegicus 208-212 26021821-6 2015 Glucagon partially inhibited carbachol-induced tracheal contraction in a mechanism clearly sensitive to des-His1-[Glu9]-glucagon amide, a GcgR antagonist. Carbachol 29-38 glucagon receptor Mus musculus 138-142 25911613-4 2015 Carbachol-induced vasodilatation was increased in arteries from the RBP4-KO compared with the WT mice and was impaired in the RBP4-Tg mice. Carbachol 0-9 retinol binding protein 4, plasma Mus musculus 68-72 25911613-4 2015 Carbachol-induced vasodilatation was increased in arteries from the RBP4-KO compared with the WT mice and was impaired in the RBP4-Tg mice. Carbachol 0-9 retinol binding protein 4, plasma Mus musculus 126-130 25680367-6 2015 At the cellular level, carbachol induced an increase in the intracellular [Ca(2+)] that was more than 2-fold larger in PG and SMG than in SLG acinar cells. Carbachol 23-32 small nuclear ribonucleoprotein polypeptide G Mus musculus 126-129 24974903-9 2015 Ano1 is located in a basolateral compartment/membrane rather than in the apical membrane, where it supports CCH-induced Ca(2+) increase, while the essential and possibly only apical Cl(-) channel is CFTR. Carbachol 108-111 anoctamin 1, calcium activated chloride channel Mus musculus 0-4 26084221-8 2015 The decrease observed in the CCh-stimulated HCO3(-) response in M4 KO mice was reversed by the co-application of CYN154806, a somatostatin receptor type 2 (SST2) antagonist. Carbachol 29-32 somatostatin receptor 2 Mus musculus 126-154 26084221-8 2015 The decrease observed in the CCh-stimulated HCO3(-) response in M4 KO mice was reversed by the co-application of CYN154806, a somatostatin receptor type 2 (SST2) antagonist. Carbachol 29-32 somatostatin receptor 2 Mus musculus 156-160 26084221-9 2015 Octreotide (a somatostatin analogue) decreased the basal and CCh-stimulated secretion of HCO3(-) in wild-type mice. Carbachol 61-64 somatostatin Mus musculus 14-26 25680367-7 2015 Carbachol-stimulated Cl(-) efflux and the protein levels of the Ca(2+)-activated Cl(-) channel TMEM16A, the major apical Cl(-) efflux pathway in salivary acinar cells, were significantly greater in PG compared with SMG and SLG. Carbachol 0-9 anoctamin 1, calcium activated chloride channel Mus musculus 95-102 25680367-7 2015 Carbachol-stimulated Cl(-) efflux and the protein levels of the Ca(2+)-activated Cl(-) channel TMEM16A, the major apical Cl(-) efflux pathway in salivary acinar cells, were significantly greater in PG compared with SMG and SLG. Carbachol 0-9 small nuclear ribonucleoprotein polypeptide G Mus musculus 215-218 25639149-10 2015 We also found that desipramine inhibits the increase in membrane aquaporin-5 level triggered by carbachol and histamine treatments. Carbachol 96-105 aquaporin 5 Homo sapiens 65-76 25910195-10 2015 These mice exhibited endothelial dysfunction, increased amyloid-beta in cerebral microvessels, decreases in carbachol-induced pCREB and pERK formation in hippocampal slices, and brain atrophy. Carbachol 108-117 eukaryotic translation initiation factor 2 alpha kinase 3 Mus musculus 136-140 24867682-3 2015 We used the mAChR agonist, carbachol, to determine the changes in KCa1.1 channel activity upon mAChR activation in freshly isolated human DSM cells obtained from open bladder surgeries using the perforated whole cell and single KCa1.1 channel patch-clamp recordings. Carbachol 27-36 potassium calcium-activated channel subfamily M alpha 1 Homo sapiens 66-72 24867682-5 2015 Carbachol inhibited the amplitude and frequency of KCa1.1 channel-mediated spontaneous transient outward currents and spontaneous transient hyperpolarizations, which are triggered by the release of Ca(2+) from ryanodine receptors. Carbachol 0-9 potassium calcium-activated channel subfamily M alpha 1 Homo sapiens 51-57 24867682-6 2015 Carbachol also caused membrane potential depolarization, which was not observed in the presence of iberiotoxin, a KCa1.1 channel inhibitor, indicating the critical role of the KCa1.1 channels. Carbachol 0-9 potassium calcium-activated channel subfamily M alpha 1 Homo sapiens 114-120 24867682-6 2015 Carbachol also caused membrane potential depolarization, which was not observed in the presence of iberiotoxin, a KCa1.1 channel inhibitor, indicating the critical role of the KCa1.1 channels. Carbachol 0-9 potassium calcium-activated channel subfamily M alpha 1 Homo sapiens 176-182 24867682-7 2015 The potential direct carbachol effects on KCa1.1 channels were examined under conditions of removing the major cellular Ca(2+) sources for KCa1.1 channel activation with pharmacological inhibitors (thapsigargin, ryanodine, and nifedipine). Carbachol 21-30 potassium calcium-activated channel subfamily M alpha 1 Homo sapiens 42-48 25730677-8 2015 A marked but not full overlap was observed: all neurons depolarized by carbachol were depolarized by the CB1 receptor agonist ACEA, and all neurons lacking response to carbachol lacked response to ACEA as well. Carbachol 71-80 cannabinoid receptor 1 (brain) Mus musculus 105-108 25567809-5 2015 l-cysteine, an activator of CSE, and NaHS, a donor of H2S, inhibited carbachol-induced Rho kinase and PKC activity, Rho kinase-sensitive phosphorylation of MYPT1, PKC-sensitive phosphorylation of CPI-17, and MLC20 phosphorylation and sustained muscle contraction. Carbachol 69-78 protein phosphatase 1 regulatory subunit 12A Homo sapiens 156-161 25567809-5 2015 l-cysteine, an activator of CSE, and NaHS, a donor of H2S, inhibited carbachol-induced Rho kinase and PKC activity, Rho kinase-sensitive phosphorylation of MYPT1, PKC-sensitive phosphorylation of CPI-17, and MLC20 phosphorylation and sustained muscle contraction. Carbachol 69-78 protein phosphatase 1 regulatory inhibitor subunit 14A Homo sapiens 196-202 25567809-5 2015 l-cysteine, an activator of CSE, and NaHS, a donor of H2S, inhibited carbachol-induced Rho kinase and PKC activity, Rho kinase-sensitive phosphorylation of MYPT1, PKC-sensitive phosphorylation of CPI-17, and MLC20 phosphorylation and sustained muscle contraction. Carbachol 69-78 myosin light chain 12B Homo sapiens 208-213 25398705-8 2015 By analyzing tear secretion induced with carbachol in presence of a P2Y2 receptor siRNA, we found that tear secretion was diminished to 60 %. Carbachol 41-50 LOC100009486 Oryctolagus cuniculus 68-81 25421240-4 2014 Stimulation with the cholinergic agonist carbachol leads to activation of the MAP kinase extracellular signal regulated kinase, together with the protein kinase Akt. Carbachol 41-50 AKT serine/threonine kinase 1 Homo sapiens 161-164 25537671-7 2015 The contractility in response to carbachol was significantly decreased in the proximal colon of IL-10(-/-) mice compared to IL-10(+/+) mice, but no significant difference was found in the distal colon. Carbachol 33-42 interleukin 10 Mus musculus 96-101 25537671-7 2015 The contractility in response to carbachol was significantly decreased in the proximal colon of IL-10(-/-) mice compared to IL-10(+/+) mice, but no significant difference was found in the distal colon. Carbachol 33-42 interleukin 10 Mus musculus 124-129 25557225-9 2015 Application of ML-7 (MLCK inhibitor) during CCh-induced sustained contraction elicited an MLCP-dependent relaxation, the rate of which was accelerated by application of PD98059 and SB203580 with proportional changes in LC20 phosphorylation levels but not MYPT1 phosphorylation (Thr697 or Thr855). Carbachol 44-47 myosin light chain kinase Rattus norvegicus 21-25 25557225-10 2015 CONCLUSIONS & INFERENCES: ERK and p38MAPK contribute to CCh-induced sustained contraction in a LC20 phosphorylation dependent manner. Carbachol 60-63 Eph receptor B1 Rattus norvegicus 30-33 25660359-0 2015 beta-Adrenoceptor-Mediated Relaxation of Carbachol-Pre-Contracted Mouse Detrusor. Carbachol 41-50 adrenergic receptor, beta 1 Mus musculus 0-17 25660359-1 2015 AIMS: To study the beta-adrenoceptor subtypes involved in the relaxation responses to (-)-isoprenaline in carbachol-pre-contracted (CCh) mouse detrusor muscle with intact and denuded mucosa. Carbachol 106-115 adrenergic receptor, beta 1 Mus musculus 19-36 26068049-4 2015 KCl- or carbachol-precontracted strips were relaxed with increasing concentrations of noradrenaline in the absence and in the presence of nitric oxide synthase inhibitor, L-NAME; P2X-receptor antagonist, PPADS; ETA-receptor antagonist, BQ-123; ETB-receptor antagonist, BQ-788; cyclooxygenase inhibitor, diclofenac; AT1-receptor antagonist, candesartan; and NK1-receptor antagonist, L-703,606. Carbachol 8-17 tachykinin receptor 1 Homo sapiens 357-369 25242372-7 2014 CCh triggered the activation of the endoplasmic reticulum Ca(2+) sensor stromal interaction molecule (STIM) 1, as indicated by redistribution of STIM1 immunofluorescence into puncta, and promoted the association of STIM1 with the SOCE channel component Orai1. Carbachol 0-3 stromal interaction molecule 1 Homo sapiens 72-109 25242372-7 2014 CCh triggered the activation of the endoplasmic reticulum Ca(2+) sensor stromal interaction molecule (STIM) 1, as indicated by redistribution of STIM1 immunofluorescence into puncta, and promoted the association of STIM1 with the SOCE channel component Orai1. Carbachol 0-3 stromal interaction molecule 1 Homo sapiens 145-150 25242372-7 2014 CCh triggered the activation of the endoplasmic reticulum Ca(2+) sensor stromal interaction molecule (STIM) 1, as indicated by redistribution of STIM1 immunofluorescence into puncta, and promoted the association of STIM1 with the SOCE channel component Orai1. Carbachol 0-3 stromal interaction molecule 1 Homo sapiens 215-220 25242372-7 2014 CCh triggered the activation of the endoplasmic reticulum Ca(2+) sensor stromal interaction molecule (STIM) 1, as indicated by redistribution of STIM1 immunofluorescence into puncta, and promoted the association of STIM1 with the SOCE channel component Orai1. Carbachol 0-3 ORAI calcium release-activated calcium modulator 1 Homo sapiens 253-258 25242372-8 2014 Cell depletion of STIM1 by siRNA treatment reduced both CCh-induced [Ca(2+)]i plateau and AMPK activation. Carbachol 56-59 stromal interaction molecule 1 Homo sapiens 18-23 25431134-1 2015 Parathyroid hormone (PTH) stimulates adenylyl cyclase through type 1 PTH receptors (PTH1R) and potentiates the Ca(2+) signals evoked by carbachol, which stimulates formation of inositol 1,4,5-trisphosphate (IP3). Carbachol 136-145 parathyroid hormone Homo sapiens 0-19 25431134-1 2015 Parathyroid hormone (PTH) stimulates adenylyl cyclase through type 1 PTH receptors (PTH1R) and potentiates the Ca(2+) signals evoked by carbachol, which stimulates formation of inositol 1,4,5-trisphosphate (IP3). Carbachol 136-145 parathyroid hormone Homo sapiens 21-24 25431134-2 2015 We confirmed that in HEK cells expressing PTH1R, acute stimulation with PTH(1-34) potentiated carbachol-evoked Ca(2+) release. Carbachol 94-103 parathyroid hormone 1 receptor Homo sapiens 42-47 25431134-2 2015 We confirmed that in HEK cells expressing PTH1R, acute stimulation with PTH(1-34) potentiated carbachol-evoked Ca(2+) release. Carbachol 94-103 parathyroid hormone Homo sapiens 42-45 25431134-5 2015 Here, we show that sustained stimulation with PTH(1-34) or with PTH analogues that do not evoke receptor internalization reduced the potentiated Ca(2+) signals and attenuated carbachol-evoked increases in cytosolic IP3. Carbachol 175-184 parathyroid hormone Homo sapiens 46-49 25431134-5 2015 Here, we show that sustained stimulation with PTH(1-34) or with PTH analogues that do not evoke receptor internalization reduced the potentiated Ca(2+) signals and attenuated carbachol-evoked increases in cytosolic IP3. Carbachol 175-184 parathyroid hormone Homo sapiens 64-67 25234725-9 2014 Results suggest that acetylcholine and short-term electrical stimulation reduce GDNF secretion, while treatment with carbachol or long-term electrical stimulation enhances GDNF production by skeletal muscle. Carbachol 117-126 glial cell derived neurotrophic factor Homo sapiens 172-176 24846346-4 2014 RESULTS: In bladder strips from non-diabetic animals, the presence of the urothelium resulted in marked sensitivity to carbachol-induced force generation by modulators of MaxiK and Kv7 channel activity, whereas in the diabetic animal urothelial sensitivity to these agents was significantly diminished. Carbachol 119-128 potassium calcium-activated channel subfamily M alpha 1 Homo sapiens 171-176 24990429-7 2014 KEY RESULTS: The cholinoceptor agonist carbachol was more effective at stimulating proliferation in iNIH3T3 than in NIH3T3 cells, probably due to the de novo induction of M3 and M5 muscarinic receptors independently of NF-kappaB activation. Carbachol 39-48 nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 Mus musculus 219-228 24990429-11 2014 Inflammation also up-regulated the expression of NOS and COX-2, thus potentiating the effect of carbachol on NO and PGE2 production. Carbachol 96-105 cytochrome c oxidase II, mitochondrial Mus musculus 57-62 25230765-7 2014 Ghrelin enhanced smooth muscle strip contraction induced by CCh, but when CCh was absent, this effect was eliminated. Carbachol 60-63 ghrelin and obestatin prepropeptide Rattus norvegicus 0-7 25230765-11 2014 In conclusion the present study demonstrated that ghrelin may act as an adjuvant to regulate gastric smooth muscle contraction induced by CCh through GHS-R1s, which are expressed on myenteric nerve cells, Cajal cells and smooth muscle cells. Carbachol 138-141 ghrelin and obestatin prepropeptide Rattus norvegicus 50-57 25375115-9 2014 Menthol (300 microM) or nifedipine (1 microM) inhibited carbachol and EFS-induced contractions in both wild type and TRPM8 knockout bladder strips. Carbachol 56-65 transient receptor potential cation channel, subfamily M, member 8 Mus musculus 117-122 26417327-10 2014 Additionally, the blockade of Ox1r in the VTA by SB334867 can attenuate the conditioning score induced by concurrent administration of carbachol and an ineffective dose of morphine. Carbachol 135-144 hypocretin receptor 1 Rattus norvegicus 30-34 25031220-5 2014 Results indicate that mGluR5 activation promotes feed-forward inhibition that depends on recruitment of neuronal activity by carbachol-evoked up states. Carbachol 125-134 glutamate receptor, ionotropic, kainate 1 Mus musculus 22-28 25031220-6 2014 The rate of neuronal spiking activity under the influence of carbachol was reduced by the mGluR5 positive allosteric modulator, N-(1,3-Diphenyl-1H-pyrazolo-5-yl)-4-nitrobenzamide (VU-29), and enhanced by the mGluR5 negative allosteric modulator, 3-((2-methyl-1,3-thiazol-4-yl)ethynyl)pyridine hydrochloride (MTEP). Carbachol 61-70 glutamate receptor, ionotropic, kainate 1 Mus musculus 90-96 25031220-6 2014 The rate of neuronal spiking activity under the influence of carbachol was reduced by the mGluR5 positive allosteric modulator, N-(1,3-Diphenyl-1H-pyrazolo-5-yl)-4-nitrobenzamide (VU-29), and enhanced by the mGluR5 negative allosteric modulator, 3-((2-methyl-1,3-thiazol-4-yl)ethynyl)pyridine hydrochloride (MTEP). Carbachol 61-70 glutamate receptor, ionotropic, kainate 1 Mus musculus 208-214 25106095-4 2014 In neonatal rat ventricular myocytes (NRVMs), sphingosine 1-phosphate (S1P), lysophosphatidic acid (LPA) and endothelin-1 induced robust increases in CCN1 expression while phenylephrine, isoproterenol and carbachol had little or no effect. Carbachol 205-214 endothelin 1 Rattus norvegicus 109-121 24628494-10 2014 Based on this highly reliable assay, 50% of the pSS patients had antibodies which inhibited carbachol-induced activation of mAchR3; none of the SSc patients, 6% of the patients with MG and 12% of the blood donors had antibodies which reacted with the mAchR3. Carbachol 92-101 cholinergic receptor, nicotinic, gamma polypeptide Mus musculus 124-130 24835173-5 2014 Isolated MYPT1-deficient tissues from MYPT1(SM-/-) mice contracted with moderate differences in response to KCl and carbachol treatments, and relaxed rapidly with comparable rates after carbachol removal and only 1.5-fold slower after KCl removal. Carbachol 116-125 protein phosphatase 1, regulatory subunit 12A Mus musculus 9-14 24835173-5 2014 Isolated MYPT1-deficient tissues from MYPT1(SM-/-) mice contracted with moderate differences in response to KCl and carbachol treatments, and relaxed rapidly with comparable rates after carbachol removal and only 1.5-fold slower after KCl removal. Carbachol 186-195 protein phosphatase 1, regulatory subunit 12A Mus musculus 9-14 24928903-5 2014 Carbachol (CCH) and forskolin (FSK) stimulated NHE3 endocytosis in control but not in myosin VI KD cells. Carbachol 0-9 solute carrier family 9 member A3 Homo sapiens 47-51 24928903-5 2014 Carbachol (CCH) and forskolin (FSK) stimulated NHE3 endocytosis in control but not in myosin VI KD cells. Carbachol 11-14 solute carrier family 9 member A3 Homo sapiens 47-51 24828459-5 2014 SMG innervation could be restored by the acetylcholine analog carbachol (CCh), which also rescued cytokeratin 5 (CK5(+))-expressing epithelial progenitor cells. Carbachol 62-71 keratin 5 Homo sapiens 98-111 24828459-5 2014 SMG innervation could be restored by the acetylcholine analog carbachol (CCh), which also rescued cytokeratin 5 (CK5(+))-expressing epithelial progenitor cells. Carbachol 73-76 keratin 5 Homo sapiens 98-111 24628494-10 2014 Based on this highly reliable assay, 50% of the pSS patients had antibodies which inhibited carbachol-induced activation of mAchR3; none of the SSc patients, 6% of the patients with MG and 12% of the blood donors had antibodies which reacted with the mAchR3. Carbachol 92-101 cholinergic receptor, nicotinic, gamma polypeptide Mus musculus 251-257 24522860-4 2014 RESULTS: Activation of M3 mAChR with carbachol increased both IL-8 mRNA and protein expression in a concentration-dependent manner. Carbachol 37-46 C-X-C motif chemokine ligand 8 Homo sapiens 62-66 24867958-0 2014 NHERF2/NHERF3 protein heterodimerization and macrocomplex formation are required for the inhibition of NHE3 activity by carbachol. Carbachol 120-129 SLC9A3 regulator 2 Homo sapiens 0-6 24867958-0 2014 NHERF2/NHERF3 protein heterodimerization and macrocomplex formation are required for the inhibition of NHE3 activity by carbachol. Carbachol 120-129 PDZ domain containing 1 Homo sapiens 7-13 24867958-0 2014 NHERF2/NHERF3 protein heterodimerization and macrocomplex formation are required for the inhibition of NHE3 activity by carbachol. Carbachol 120-129 solute carrier family 9 member A3 Homo sapiens 103-107 24867958-11 2014 This study suggests that NHERF2/NHERF3 heterodimerization mediates the formation of NHE3 macrocomplexes, which are required for the inhibition of NHE3 activity by carbachol. Carbachol 163-172 SLC9A3 regulator 2 Homo sapiens 25-31 24867958-11 2014 This study suggests that NHERF2/NHERF3 heterodimerization mediates the formation of NHE3 macrocomplexes, which are required for the inhibition of NHE3 activity by carbachol. Carbachol 163-172 PDZ domain containing 1 Homo sapiens 32-38 24867958-11 2014 This study suggests that NHERF2/NHERF3 heterodimerization mediates the formation of NHE3 macrocomplexes, which are required for the inhibition of NHE3 activity by carbachol. Carbachol 163-172 solute carrier family 9 member A3 Homo sapiens 84-88 24867958-11 2014 This study suggests that NHERF2/NHERF3 heterodimerization mediates the formation of NHE3 macrocomplexes, which are required for the inhibition of NHE3 activity by carbachol. Carbachol 163-172 solute carrier family 9 member A3 Homo sapiens 146-150 24522860-7 2014 Furthermore, M3 mAChR-mediated NF-kappaB activation and IL-8 expression were simultaneously attenuated by the PKC inhibitor calphostin C, whereas PMA, a PKC activator, mimicked the effects of carbachol, inducing IL-8 expression. Carbachol 192-201 proline rich transmembrane protein 2 Homo sapiens 110-113 24688054-6 2014 Treatment of cells with carbachol reduced the hERG-ubiquitin interaction and slowed the rate of hERG degradation. Carbachol 24-33 ETS transcription factor ERG Homo sapiens 46-50 24688054-6 2014 Treatment of cells with carbachol reduced the hERG-ubiquitin interaction and slowed the rate of hERG degradation. Carbachol 24-33 ETS transcription factor ERG Homo sapiens 96-100 24688054-8 2014 Here, we found that disrupting the Nedd4-2 binding domain in hERG completely eliminated the effect of carbachol on hERG channels. Carbachol 102-111 NEDD4 like E3 ubiquitin protein ligase Homo sapiens 35-42 24688054-8 2014 Here, we found that disrupting the Nedd4-2 binding domain in hERG completely eliminated the effect of carbachol on hERG channels. Carbachol 102-111 ETS transcription factor ERG Homo sapiens 61-65 24688054-8 2014 Here, we found that disrupting the Nedd4-2 binding domain in hERG completely eliminated the effect of carbachol on hERG channels. Carbachol 102-111 ETS transcription factor ERG Homo sapiens 115-119 24688054-9 2014 Carbachol treatment enhanced the phosphorylation level, but not the total level, of Nedd4-2. Carbachol 0-9 NEDD4 like E3 ubiquitin protein ligase Homo sapiens 84-91 24688054-10 2014 Blockade of the protein kinase C (PKC) pathway abolished the carbachol-induced enhancement of hERG channels. Carbachol 61-70 ETS transcription factor ERG Homo sapiens 94-98 24487025-8 2014 Parasympathetic nerve activation by carbachol infusion for 5 days in rats increased serum adiponectin, with increased adiponectin production in visceral and subcutaneous adipose tissues without changes of body weight. Carbachol 36-45 adiponectin, C1Q and collagen domain containing Rattus norvegicus 90-101 24652077-3 2014 The effect of CB1 agonist (ACEA) on carbachol- and ATP-induced changes in intracellular calcium ([Ca(2+)]i) levels was measured using fluorimetry. Carbachol 36-45 cannabinoid receptor 1 Homo sapiens 14-17 24623142-8 2014 Therefore, the increase in CCh-induced fluid secretion in response to hypotonic conditions can be attributed, to a large extent, to the specific activation of the NKCC1. Carbachol 27-30 solute carrier family 12, member 2 Mus musculus 163-168 24688054-4 2014 Using cell biology and electrophysiological methods, we found that the muscarinic receptor agonist carbachol increased the expression and function of hERG, but not ether-a-go-go or Kv1.5 channels stably expressed in human embryonic kidney cells. Carbachol 99-108 ETS transcription factor ERG Homo sapiens 150-154 24688054-5 2014 The carbachol-mediated increase in hERG expression was abolished by the selective M3 antagonist 4-DAMP (1,1-dimethyl-4-diphenylacetoxypiperidinium iodide) but not by the M2 antagonist AF-DX 116 (11[[2-[(diethylamino)methyl]-1-piperidinyl]-acetyl]-5,11-dihydro-6H-pyrido[2,3-b] [1,4]benzodiazepine-6-one). Carbachol 4-13 ETS transcription factor ERG Homo sapiens 35-39 24695728-3 2014 The muscarinic receptor agonist carbachol activated ERK1/2 better in T1R3-depleted cells than in control cells. Carbachol 32-41 mitogen-activated protein kinase 3 Mus musculus 52-58 24695728-3 2014 The muscarinic receptor agonist carbachol activated ERK1/2 better in T1R3-depleted cells than in control cells. Carbachol 32-41 taste receptor, type 1, member 3 Mus musculus 69-73 24487025-8 2014 Parasympathetic nerve activation by carbachol infusion for 5 days in rats increased serum adiponectin, with increased adiponectin production in visceral and subcutaneous adipose tissues without changes of body weight. Carbachol 36-45 adiponectin, C1Q and collagen domain containing Rattus norvegicus 118-129 24586057-3 2014 Here, we show that, in addition to suppressing carbachol-stimulated Ca(2+) release, Gbeta5-RGS7 enhanced Ca(2+) influx. Carbachol 47-56 G protein subunit beta 5 Homo sapiens 84-90 24608858-6 2014 In addition, overexpression of DGKeta enhanced calcium mobilization after stimulating muscarinic receptors with carbachol and after stimulating purinergic receptors with ATP. Carbachol 112-121 diacylglycerol kinase eta Homo sapiens 31-37 24608858-8 2014 DGKeta was localized throughout the cytosol and did not translocate to the plasma membrane after stimulation with carbachol. Carbachol 114-123 diacylglycerol kinase eta Homo sapiens 0-6 24219573-8 2014 Human precision-cut lung slices treated with IL-13 caused a decrease in beta-agonist (formoterol)-mediated relaxation of carbachol-contracted airways compared with control slices. Carbachol 121-130 interleukin 13 Homo sapiens 45-50 24365239-7 2014 Carbachol (CCh) increased ERK and FAK phosphorylation with enhanced TER recovery, which was completely blocked by either MT-7 (M1 antagonist) or atropine. Carbachol 0-9 mitogen-activated protein kinase 1 Homo sapiens 26-29 24365239-7 2014 Carbachol (CCh) increased ERK and FAK phosphorylation with enhanced TER recovery, which was completely blocked by either MT-7 (M1 antagonist) or atropine. Carbachol 0-9 protein tyrosine kinase 2 Homo sapiens 34-37 24365239-7 2014 Carbachol (CCh) increased ERK and FAK phosphorylation with enhanced TER recovery, which was completely blocked by either MT-7 (M1 antagonist) or atropine. Carbachol 11-14 mitogen-activated protein kinase 1 Homo sapiens 26-29 24365239-7 2014 Carbachol (CCh) increased ERK and FAK phosphorylation with enhanced TER recovery, which was completely blocked by either MT-7 (M1 antagonist) or atropine. Carbachol 11-14 protein tyrosine kinase 2 Homo sapiens 34-37 24365239-8 2014 The CCh-induced enhancement of TER recovery was also blocked by either U0126 (ERK pathway inhibitor) or PF-228 (FAK inhibitor). Carbachol 4-7 mitogen-activated protein kinase 1 Homo sapiens 78-81 24365239-8 2014 The CCh-induced enhancement of TER recovery was also blocked by either U0126 (ERK pathway inhibitor) or PF-228 (FAK inhibitor). Carbachol 4-7 protein tyrosine kinase 2 Homo sapiens 112-115 24365239-10 2014 The CCh-induced ERK and FAK phosphorylation were also attenuated by the IFN-gamma treatment. Carbachol 4-7 mitogen-activated protein kinase 1 Homo sapiens 16-19 24365239-10 2014 The CCh-induced ERK and FAK phosphorylation were also attenuated by the IFN-gamma treatment. Carbachol 4-7 protein tyrosine kinase 2 Homo sapiens 24-27 24365239-10 2014 The CCh-induced ERK and FAK phosphorylation were also attenuated by the IFN-gamma treatment. Carbachol 4-7 interferon gamma Homo sapiens 72-81 24678619-8 2014 Moreover, carbachol induced TGF-beta1 production from A549 cells concomitantly with the EMT process. Carbachol 10-19 transforming growth factor beta 1 Homo sapiens 28-37 24678619-9 2014 Carbachol-induced EMT occurred through phosphorylation of Smad2/3 and ERK, which was inhibited by pirenzepine and 4-DAMP. Carbachol 0-9 SMAD family member 2 Homo sapiens 58-65 24678619-9 2014 Carbachol-induced EMT occurred through phosphorylation of Smad2/3 and ERK, which was inhibited by pirenzepine and 4-DAMP. Carbachol 0-9 mitogen-activated protein kinase 1 Homo sapiens 70-73 24642792-5 2014 The conventional and novel PKC inhibitors Go6976 or Go6850 did not alter NBC function or surface expression by themselves, but stimulation with forskolin (10(-5) M) or carbachol (10(-4) M) in their presence led to a significant decrease in NBC-mediated proton flux, and biotinylated NBCe1. Carbachol 168-177 solute carrier family 4 (anion exchanger), member 4 Mus musculus 240-243 24607024-9 2014 RESULTS: Prolonged culture of ASMCs with acetylcholine, carbachol or FBS, reduced the expression of alpha-actin, desmin and SM-MHC compared to cells cultured in serum free medium. Carbachol 56-65 desmin Oryctolagus cuniculus 113-119 24607024-9 2014 RESULTS: Prolonged culture of ASMCs with acetylcholine, carbachol or FBS, reduced the expression of alpha-actin, desmin and SM-MHC compared to cells cultured in serum free medium. Carbachol 56-65 myosin-11 Oryctolagus cuniculus 124-130 24485888-5 2014 Activation of Galphaq proteins was also detectable by the addition of carbachol via muscarinic acetylcholine M1 receptors, (-)-epinephrine, and dopamine, but not by L-glutamate or (+-)-baclofen. Carbachol 70-79 G protein subunit alpha q Rattus norvegicus 14-21 24356881-4 2014 The aim of this study was to identify the role of BDNF in carbachol (CCh)- and substance P (SP)-induced contraction of intestinal longitudinal smooth muscle. Carbachol 58-67 LOW QUALITY PROTEIN: brain-derived neurotrophic factor Oryctolagus cuniculus 50-54 24630173-5 2014 In contrast, Ca(2+) signals to carbachol were significantly increased in ApoE(-/-) cells, an effect methyl-beta-cyclodextrin reversed. Carbachol 31-40 apolipoprotein E Mus musculus 73-77 24630173-9 2014 In conclusion, carbachol-induced calcium signalling and handling are significantly altered in endothelial cells of ApoE(-/-) mice before plaque development. Carbachol 15-24 apolipoprotein E Mus musculus 115-119 24389807-8 2014 Carbachol (10 microM) increased the ratio of non-lipid microdomain to total AQP5 in the cultured control submandibular gland tissue. Carbachol 0-9 aquaporin 5 Homo sapiens 76-80 24558448-7 2014 3/Wash-resistant xanomeline selectively prevented further increase in intracellular calcium by carbachol at hM1 and hM4 receptors. Carbachol 95-104 cholinergic receptor muscarinic 1 Homo sapiens 108-111 24356881-4 2014 The aim of this study was to identify the role of BDNF in carbachol (CCh)- and substance P (SP)-induced contraction of intestinal longitudinal smooth muscle. Carbachol 69-72 LOW QUALITY PROTEIN: brain-derived neurotrophic factor Oryctolagus cuniculus 50-54 24356881-7 2014 One-hour preincubation with BDNF enhanced intestinal muscle contraction induced by CCh but not by SP. Carbachol 83-86 LOW QUALITY PROTEIN: brain-derived neurotrophic factor Oryctolagus cuniculus 28-32 24356881-10 2014 The enhancement of CCh-induced contraction by BDNF was blocked by the phospholipase C (PLC) antagonist U73122, but not by ERK1/2 or Akt antagonists. Carbachol 19-22 LOW QUALITY PROTEIN: brain-derived neurotrophic factor Oryctolagus cuniculus 46-50 24356881-10 2014 The enhancement of CCh-induced contraction by BDNF was blocked by the phospholipase C (PLC) antagonist U73122, but not by ERK1/2 or Akt antagonists. Carbachol 19-22 LOC100009319 Oryctolagus cuniculus 70-85 24356881-10 2014 The enhancement of CCh-induced contraction by BDNF was blocked by the phospholipase C (PLC) antagonist U73122, but not by ERK1/2 or Akt antagonists. Carbachol 19-22 LOC100009319 Oryctolagus cuniculus 87-90 24356881-12 2014 We conclude that exogenous BDNF augments the CCh-induced contraction of longitudinal muscle from rabbit intestine by activating TrkB receptors and subsequent PLC activation. Carbachol 45-48 LOW QUALITY PROTEIN: brain-derived neurotrophic factor Oryctolagus cuniculus 27-31 24356881-12 2014 We conclude that exogenous BDNF augments the CCh-induced contraction of longitudinal muscle from rabbit intestine by activating TrkB receptors and subsequent PLC activation. Carbachol 45-48 LOC100009319 Oryctolagus cuniculus 158-161 23827485-7 2014 With human bronchial rings, we observed that whatever the compound used including salbutamol, the activation of muscular CFTR leads to a bronchodilation after constriction with carbachol. Carbachol 177-186 CF transmembrane conductance regulator Homo sapiens 121-125 24269928-6 2014 Carbachol concentration-dependently enhanced the production of IL-1beta-induced IL-6 and IL-8, which was blocked by the simultaneous addition of tiotropium. Carbachol 0-9 interleukin 6 Homo sapiens 80-84 24269928-6 2014 Carbachol concentration-dependently enhanced the production of IL-1beta-induced IL-6 and IL-8, which was blocked by the simultaneous addition of tiotropium. Carbachol 0-9 C-X-C motif chemokine ligand 8 Homo sapiens 89-93 24269928-8 2014 Olodaterol induced cAMP and the phosphorylation of CREB, an effect counteracted by carbachol, but rescued by tiotropium. Carbachol 83-92 cAMP responsive element binding protein 1 Homo sapiens 51-55 24190932-10 2014 The low-frequency IPSC oscillations induced by CCh or optogenetically stimulated ACh release were also inhibited by a mu-opioid receptor (MOR) agonist, which was unexpected because MORs in CA1 are not usually associated with CCK-expressing cells. Carbachol 47-50 opioid receptor mu 1 Homo sapiens 118-136 24318880-6 2014 In retrogradely perfused hearts, ablation of RGS6, but not RGS4, correlated with decreased resting HR, increased heart rate variability, and enhanced sensitivity to the negative chronotropic effects of the muscarinic agonist carbachol. Carbachol 225-234 regulator of G-protein signaling 6 Mus musculus 45-49 24318880-7 2014 Similarly, loss of RGS6, but not RGS4, correlated with enhanced sensitivity of the M2R-IKACh signaling pathway in SAN cells to carbachol and a significant slowing of M2R-IKACh deactivation rate. Carbachol 127-136 regulator of G-protein signaling 6 Mus musculus 19-23 24122009-2 2014 The aim of this study was to determine the relative contributions of MLCK and ROCK to carbachol-induced contraction of human detrusor smooth muscle in vitro. Carbachol 86-95 myosin light chain kinase Homo sapiens 69-73 24122009-6 2014 Pre-incubation of detrusor specimens with either the MLCK inhibitor ML-9 or the ROCK inhibitors HA1100 and Y-27632 (each at 10 mumol/L) significantly blocked carbachol-induced contractions as compared to the time-control experiments. Carbachol 158-167 myosin light chain kinase Homo sapiens 53-57 24122009-7 2014 Moreover, MLCK and ROCK inhibition were equally effective in reducing carbachol-induced contractions. Carbachol 70-79 myosin light chain kinase Homo sapiens 10-14 24122009-8 2014 The residual carbachol-induced contractions in the presence of both MLCK and ROCK inhibitors were significantly smaller than the contractions obtained when only one enzyme (either MLCK or ROCK) was inhibited, suggesting an additive effect of the two kinases. Carbachol 13-22 myosin light chain kinase Homo sapiens 68-72 24122009-8 2014 The residual carbachol-induced contractions in the presence of both MLCK and ROCK inhibitors were significantly smaller than the contractions obtained when only one enzyme (either MLCK or ROCK) was inhibited, suggesting an additive effect of the two kinases. Carbachol 13-22 myosin light chain kinase Homo sapiens 180-184 24122009-10 2014 CONCLUSION: Both MLCK and ROCK contribute to carbachol-induced contractions of human detrusor smooth muscle. Carbachol 45-54 myosin light chain kinase Homo sapiens 17-21 24713550-0 2014 Carbachol-mediated endocytosis of NHE3 involves a clathrin-independent mechanism requiring lipid rafts and Cdc42. Carbachol 0-9 solute carrier family 9 member A3 Homo sapiens 34-38 24713550-0 2014 Carbachol-mediated endocytosis of NHE3 involves a clathrin-independent mechanism requiring lipid rafts and Cdc42. Carbachol 0-9 cell division cycle 42 Homo sapiens 107-112 24713550-3 2014 Carbachol (CCH), which elevates intracellular Ca(2+) ([Ca(2+)]i), decreases NHE3 activity and stimulates endocytosis; however, the mechanism involved in calcium-mediated endocytosis of NHE3 is unclear. Carbachol 0-9 solute carrier family 9 member A3 Homo sapiens 76-80 24713550-3 2014 Carbachol (CCH), which elevates intracellular Ca(2+) ([Ca(2+)]i), decreases NHE3 activity and stimulates endocytosis; however, the mechanism involved in calcium-mediated endocytosis of NHE3 is unclear. Carbachol 0-9 solute carrier family 9 member A3 Homo sapiens 185-189 24713550-3 2014 Carbachol (CCH), which elevates intracellular Ca(2+) ([Ca(2+)]i), decreases NHE3 activity and stimulates endocytosis; however, the mechanism involved in calcium-mediated endocytosis of NHE3 is unclear. Carbachol 11-14 solute carrier family 9 member A3 Homo sapiens 76-80 24713550-3 2014 Carbachol (CCH), which elevates intracellular Ca(2+) ([Ca(2+)]i), decreases NHE3 activity and stimulates endocytosis; however, the mechanism involved in calcium-mediated endocytosis of NHE3 is unclear. Carbachol 11-14 solute carrier family 9 member A3 Homo sapiens 185-189 24713550-9 2014 In contrast, CCH-inhibition of NHE3 activity was abolished in Caco-2/BBe cells treated with MbetaCD (to disrupt lipid rafts) as well as in Cdc42 knockdown cells but was unaffected by CME blockers. Carbachol 13-16 solute carrier family 9 member A3 Homo sapiens 31-35 24713550-9 2014 In contrast, CCH-inhibition of NHE3 activity was abolished in Caco-2/BBe cells treated with MbetaCD (to disrupt lipid rafts) as well as in Cdc42 knockdown cells but was unaffected by CME blockers. Carbachol 13-16 cell division cycle 42 Homo sapiens 139-144 24713550-10 2014 CONCLUSION: CCH-mediated inhibition of NHE3 activity is not dependent on clathrin and involves lipid rafts and requires Cdc42. Carbachol 12-15 solute carrier family 9 member A3 Homo sapiens 39-43 24713550-10 2014 CONCLUSION: CCH-mediated inhibition of NHE3 activity is not dependent on clathrin and involves lipid rafts and requires Cdc42. Carbachol 12-15 cell division cycle 42 Homo sapiens 120-125 24604007-0 2014 The C. elegans VIG-1 and FRM-1 modulate carbachol-stimulated ERK1/2 activation in chinese hamster ovary cells expressing the muscarinic acetylcholine receptor GAR-3. Carbachol 40-49 HABP4_PAI-RBP1 domain-containing protein Caenorhabditis elegans 15-20 24604007-0 2014 The C. elegans VIG-1 and FRM-1 modulate carbachol-stimulated ERK1/2 activation in chinese hamster ovary cells expressing the muscarinic acetylcholine receptor GAR-3. Carbachol 40-49 Moesin/ezrin/radixin homolog 1 Caenorhabditis elegans 25-30 24604007-0 2014 The C. elegans VIG-1 and FRM-1 modulate carbachol-stimulated ERK1/2 activation in chinese hamster ovary cells expressing the muscarinic acetylcholine receptor GAR-3. Carbachol 40-49 Muscarinic acetylcholine receptor gar-3 Caenorhabditis elegans 159-164 24604007-6 2014 When VIG-1 was transiently expressed in GAR-3/CHO cells, carbachol-stimulated ERK1/2 activation was substantially reduced. Carbachol 57-66 HABP4_PAI-RBP1 domain-containing protein Caenorhabditis elegans 5-10 24604007-6 2014 When VIG-1 was transiently expressed in GAR-3/CHO cells, carbachol-stimulated ERK1/2 activation was substantially reduced. Carbachol 57-66 Muscarinic acetylcholine receptor gar-3 Caenorhabditis elegans 40-45 24604007-7 2014 In contrast, transient expression of FRM-1 significantly enhanced carbachol-stimulated ERK1/2 activation. Carbachol 66-75 Moesin/ezrin/radixin homolog 1 Caenorhabditis elegans 37-42 25069526-7 2014 Treatment with IL-17A for 12 h and 3 days attenuated carbachol- and membrane depolarization-induced contractions in organ-cultured rat ileum. Carbachol 53-62 interleukin 17A Rattus norvegicus 15-21 25891767-9 2014 Selective inhibitors of Rho kinase, ERK1/2, CaMKK/AMPK, and CaMKII each reduced carbachol-induced contraction in the innervated muscle strips. Carbachol 80-89 mitogen activated protein kinase 3 Rattus norvegicus 36-42 25891767-11 2014 Thus unlike previously reported for isolated muscle cells where CaMKII and ERK1/2 are not involved in contraction, we conclude that the regulation of carbachol-induced contraction in innervated longitudinal muscle strips involves the interplay of Rho kinase, ERK1/2, CaMKK/AMPK, and CAMKII. Carbachol 150-159 mitogen activated protein kinase 3 Rattus norvegicus 259-265 24190932-10 2014 The low-frequency IPSC oscillations induced by CCh or optogenetically stimulated ACh release were also inhibited by a mu-opioid receptor (MOR) agonist, which was unexpected because MORs in CA1 are not usually associated with CCK-expressing cells. Carbachol 47-50 opioid receptor mu 1 Homo sapiens 138-141 24190932-10 2014 The low-frequency IPSC oscillations induced by CCh or optogenetically stimulated ACh release were also inhibited by a mu-opioid receptor (MOR) agonist, which was unexpected because MORs in CA1 are not usually associated with CCK-expressing cells. Carbachol 47-50 carbonic anhydrase 1 Homo sapiens 189-192 24190932-10 2014 The low-frequency IPSC oscillations induced by CCh or optogenetically stimulated ACh release were also inhibited by a mu-opioid receptor (MOR) agonist, which was unexpected because MORs in CA1 are not usually associated with CCK-expressing cells. Carbachol 47-50 cholecystokinin Homo sapiens 225-228 23832809-9 2013 Carbachol-stimulated Ca(2+) levels were reduced in ECs from Trpc6(-/-) mice. Carbachol 0-9 transient receptor potential cation channel, subfamily C, member 6 Mus musculus 60-65 24377382-9 2013 However, combination of Cch with other mitogens exhibited a dual effect, synergistic proliferation effect in the presence of EGF (5 ng/mL) and 5% FBS and inhibiting the proliferation induced by 10% FBS, EGF (10 ng/mL) and TNF-alpha (10 ng/mL). Carbachol 24-27 epidermal growth factor like 1 Rattus norvegicus 125-128 24377382-9 2013 However, combination of Cch with other mitogens exhibited a dual effect, synergistic proliferation effect in the presence of EGF (5 ng/mL) and 5% FBS and inhibiting the proliferation induced by 10% FBS, EGF (10 ng/mL) and TNF-alpha (10 ng/mL). Carbachol 24-27 epidermal growth factor like 1 Rattus norvegicus 203-206 24377382-9 2013 However, combination of Cch with other mitogens exhibited a dual effect, synergistic proliferation effect in the presence of EGF (5 ng/mL) and 5% FBS and inhibiting the proliferation induced by 10% FBS, EGF (10 ng/mL) and TNF-alpha (10 ng/mL). Carbachol 24-27 tumor necrosis factor Rattus norvegicus 222-231 23941257-8 2013 In HCSMC, carbachol-induced calcium transients were inhibited by OB (EC50 = 8.4 muM). Carbachol 10-19 latexin Homo sapiens 81-84 23941257-9 2013 Carbachol evoked 1-a smooth muscle depolarization (10 mV) that was antagonized by 100 muM OB; and 2-a contraction that was inhibited by OB (EC50 = 13.0 muM). Carbachol 0-9 latexin Homo sapiens 86-89 23941257-9 2013 Carbachol evoked 1-a smooth muscle depolarization (10 mV) that was antagonized by 100 muM OB; and 2-a contraction that was inhibited by OB (EC50 = 13.0 muM). Carbachol 0-9 latexin Homo sapiens 153-156 23993687-5 2013 1 muM SA could markedly inhibit carbachol (CCh)-mediated increase PKC-delta, PKC-eta, and CPI-17 mRNA but had no effect in PKC-epsilon.Treatment of ISMC with SA (1 muM, 30 min) caused a decrease in protein expression of PKC-delta. Carbachol 32-41 protein phosphatase 1, regulatory (inhibitor) subunit 14A Rattus norvegicus 90-96 23993687-5 2013 1 muM SA could markedly inhibit carbachol (CCh)-mediated increase PKC-delta, PKC-eta, and CPI-17 mRNA but had no effect in PKC-epsilon.Treatment of ISMC with SA (1 muM, 30 min) caused a decrease in protein expression of PKC-delta. Carbachol 43-46 protein phosphatase 1, regulatory (inhibitor) subunit 14A Rattus norvegicus 90-96 24295263-6 2013 RESULTS: Endothelin 1, oxytocin, prostaglandin F2alpha, norepinephrine, and phenylephrine induced dose-dependent contraction of the isolated urethral tissue, whereas acetylcholine, carbachol, and angiotensin II had no or only minor contractile effects. Carbachol 181-190 endothelin 1 Homo sapiens 9-21 24095671-5 2013 The pressor responses to ANG II (200ng/100nl) injected into the RVLM were reduced by acute (1 day) (12+-3 vs. sham lesions: 26+-4mmHg) or chronic (15 days) AV3V lesions (12+-5 vs. sham lesions: 27+-4mmHg), whereas acute or chronic AV3V lesions did not affect the pressor responses to carbachol (1nmol/100nl) injected into the RVLM. Carbachol 284-293 angiotensinogen Rattus norvegicus 25-31 23942896-8 2013 Perfusion of the entire submandibular gland with the TRPV1 agonist capsaicin (1 muM) via the submandibular artery significantly increased CCh-induced salivation, whereas perfusion with TRPM8 and TRPA1 agonists (0.5 muM WS12 and 100 muM allyl isothiocyanate) decreased it. Carbachol 138-141 transient receptor potential cation channel subfamily V member 1 Homo sapiens 53-58 24004375-8 2013 PSA-released CGRP also evokes a transient hyperpolarization in TSMCs upon the opening of KATP channels, which reduces contraction propagation but promotes the recruitment of TSMC Ca(2+) channels that underlie the delayed positive inotropic effects of CCh. Carbachol 251-254 calcitonin/calcitonin-related polypeptide, alpha Mus musculus 13-17 24141119-3 2013 In this report, we first examined whether Abeta reduces alpha-secretase activity, and showed that Abeta peptide 1-40 (0.001 and 0.01 muM) reduced the secretion of soluble amyloid precursor protein alpha (sAPPalpha) in carbachol-stimulated SH-SY5Y neuroblastoma cells. Carbachol 218-227 amyloid beta precursor protein Homo sapiens 171-196 23942896-8 2013 Perfusion of the entire submandibular gland with the TRPV1 agonist capsaicin (1 muM) via the submandibular artery significantly increased CCh-induced salivation, whereas perfusion with TRPM8 and TRPA1 agonists (0.5 muM WS12 and 100 muM allyl isothiocyanate) decreased it. Carbachol 138-141 latexin Homo sapiens 80-83 23748234-7 2013 CCh-stimulated [(35)S]GTPgammaS binding to Galphaq was inhibited by mAChR antagonists, including scopolamine, ipratropium, atropine, 4-DAMP, pirenzepine, and AF-DX 116, with a rank order of potency consistent with previous studies of M1-expressing cells. Carbachol 0-3 G protein subunit alpha q Rattus norvegicus 43-50 24076274-7 2013 To confirm our results we performed in vitro experiments on live hippocampal slices: we evaluated whether stimulation of the cholinergic system with the cholinergic receptor agonist carbachol (CCh) activated the mTOR pathway and whether the administration of the above-mentioned antagonists together with CCh could revert this activation. Carbachol 182-191 mechanistic target of rapamycin kinase Rattus norvegicus 212-216 23371862-10 2013 Adult heterozygotes, which have a reduced expression of Nmnat2 at E18.5, showed decreased responses to carbachol and electrical stimulation compared to wild-type controls. Carbachol 103-112 nicotinamide nucleotide adenylyltransferase 2 Mus musculus 56-62 24654541-7 2013 These could be attenuated by pretreatment with carbachol and arecoline at the concentration of 10 micromol/L for 12 h. Homocysteine induced apoptosis in RAECs and the molecular mechanisms were associated with the regulation of fas, fas-L and caspase-8 in the death receptor pathway, bcl-2, bcl-xL and bax in the mitochondrial pathway, caspase-12 in the endoplasmic reticulum pathway and caspase-3, caspase-6 and p53 as downstream effectors. Carbachol 47-56 Fas ligand Rattus norvegicus 232-237 24654541-7 2013 These could be attenuated by pretreatment with carbachol and arecoline at the concentration of 10 micromol/L for 12 h. Homocysteine induced apoptosis in RAECs and the molecular mechanisms were associated with the regulation of fas, fas-L and caspase-8 in the death receptor pathway, bcl-2, bcl-xL and bax in the mitochondrial pathway, caspase-12 in the endoplasmic reticulum pathway and caspase-3, caspase-6 and p53 as downstream effectors. Carbachol 47-56 caspase 8 Rattus norvegicus 242-251 24654541-7 2013 These could be attenuated by pretreatment with carbachol and arecoline at the concentration of 10 micromol/L for 12 h. Homocysteine induced apoptosis in RAECs and the molecular mechanisms were associated with the regulation of fas, fas-L and caspase-8 in the death receptor pathway, bcl-2, bcl-xL and bax in the mitochondrial pathway, caspase-12 in the endoplasmic reticulum pathway and caspase-3, caspase-6 and p53 as downstream effectors. Carbachol 47-56 BCL2, apoptosis regulator Rattus norvegicus 283-288 24654541-7 2013 These could be attenuated by pretreatment with carbachol and arecoline at the concentration of 10 micromol/L for 12 h. Homocysteine induced apoptosis in RAECs and the molecular mechanisms were associated with the regulation of fas, fas-L and caspase-8 in the death receptor pathway, bcl-2, bcl-xL and bax in the mitochondrial pathway, caspase-12 in the endoplasmic reticulum pathway and caspase-3, caspase-6 and p53 as downstream effectors. Carbachol 47-56 Bcl2-like 1 Rattus norvegicus 290-296 24654541-7 2013 These could be attenuated by pretreatment with carbachol and arecoline at the concentration of 10 micromol/L for 12 h. Homocysteine induced apoptosis in RAECs and the molecular mechanisms were associated with the regulation of fas, fas-L and caspase-8 in the death receptor pathway, bcl-2, bcl-xL and bax in the mitochondrial pathway, caspase-12 in the endoplasmic reticulum pathway and caspase-3, caspase-6 and p53 as downstream effectors. Carbachol 47-56 BCL2 associated X, apoptosis regulator Rattus norvegicus 301-304 24654541-7 2013 These could be attenuated by pretreatment with carbachol and arecoline at the concentration of 10 micromol/L for 12 h. Homocysteine induced apoptosis in RAECs and the molecular mechanisms were associated with the regulation of fas, fas-L and caspase-8 in the death receptor pathway, bcl-2, bcl-xL and bax in the mitochondrial pathway, caspase-12 in the endoplasmic reticulum pathway and caspase-3, caspase-6 and p53 as downstream effectors. Carbachol 47-56 caspase 12 Rattus norvegicus 335-345 24654541-7 2013 These could be attenuated by pretreatment with carbachol and arecoline at the concentration of 10 micromol/L for 12 h. Homocysteine induced apoptosis in RAECs and the molecular mechanisms were associated with the regulation of fas, fas-L and caspase-8 in the death receptor pathway, bcl-2, bcl-xL and bax in the mitochondrial pathway, caspase-12 in the endoplasmic reticulum pathway and caspase-3, caspase-6 and p53 as downstream effectors. Carbachol 47-56 caspase 3 Rattus norvegicus 387-396 24654541-7 2013 These could be attenuated by pretreatment with carbachol and arecoline at the concentration of 10 micromol/L for 12 h. Homocysteine induced apoptosis in RAECs and the molecular mechanisms were associated with the regulation of fas, fas-L and caspase-8 in the death receptor pathway, bcl-2, bcl-xL and bax in the mitochondrial pathway, caspase-12 in the endoplasmic reticulum pathway and caspase-3, caspase-6 and p53 as downstream effectors. Carbachol 47-56 caspase 6 Rattus norvegicus 398-407 24654541-7 2013 These could be attenuated by pretreatment with carbachol and arecoline at the concentration of 10 micromol/L for 12 h. Homocysteine induced apoptosis in RAECs and the molecular mechanisms were associated with the regulation of fas, fas-L and caspase-8 in the death receptor pathway, bcl-2, bcl-xL and bax in the mitochondrial pathway, caspase-12 in the endoplasmic reticulum pathway and caspase-3, caspase-6 and p53 as downstream effectors. Carbachol 47-56 Wistar clone pR53P1 p53 pseudogene Rattus norvegicus 412-415 23895769-6 2013 The best synchronized theta oscillations obtained after administration of 50 muM NMDA+50 muM BACL resembled theta activity induced by a bath perfusion of 50 muM carbachol. Carbachol 161-170 latexin Homo sapiens 77-80 24075004-0 2013 TRK-380, a novel selective human beta3-adrenoceptor agonist, ameliorates formalin-induced pollakiuria in rats and carbachol-induced bladder contraction in dogs. Carbachol 114-123 neurotrophic receptor tyrosine kinase 1 Rattus norvegicus 0-3 24075004-8 2013 In dogs, CCh-induced bladder contraction was dose-dependently suppressed by TRK-380; the plasma concentration required for 30% suppression of the CCh-induced bladder contraction (30% relaxation) was 4.90 ng/mL. Carbachol 9-12 neurotrophic receptor tyrosine kinase 1 Rattus norvegicus 76-79 24075004-8 2013 In dogs, CCh-induced bladder contraction was dose-dependently suppressed by TRK-380; the plasma concentration required for 30% suppression of the CCh-induced bladder contraction (30% relaxation) was 4.90 ng/mL. Carbachol 146-149 neurotrophic receptor tyrosine kinase 1 Rattus norvegicus 76-79 24023725-14 2013 Interestingly, the MEK blocker did reduce carbachol-mediated cleaved caspase 3 expression in HEK293-M1 cells. Carbachol 42-51 mitogen-activated protein kinase kinase 7 Homo sapiens 19-22 23895769-6 2013 The best synchronized theta oscillations obtained after administration of 50 muM NMDA+50 muM BACL resembled theta activity induced by a bath perfusion of 50 muM carbachol. Carbachol 161-170 latexin Homo sapiens 89-92 23895769-6 2013 The best synchronized theta oscillations obtained after administration of 50 muM NMDA+50 muM BACL resembled theta activity induced by a bath perfusion of 50 muM carbachol. Carbachol 161-170 latexin Homo sapiens 89-92 23784542-0 2013 Carbachol-induced MUC17 endocytosis is concomitant with NHE3 internalization and CFTR membrane recruitment in enterocytes. Carbachol 0-9 mucin 17, cell surface associated Homo sapiens 18-23 24040112-9 2013 CFTR dependent currents following 18-24 hours of cold storage for forskolin/IBMX, carbachol, and forskolin/IBMX+carbachol stimulation (n=17 non-CF subjects) were 44%, 47.5%, and 47.3%, respectively of those in fresh biopsies. Carbachol 82-91 CF transmembrane conductance regulator Homo sapiens 0-4 24040112-9 2013 CFTR dependent currents following 18-24 hours of cold storage for forskolin/IBMX, carbachol, and forskolin/IBMX+carbachol stimulation (n=17 non-CF subjects) were 44%, 47.5%, and 47.3%, respectively of those in fresh biopsies. Carbachol 112-121 CF transmembrane conductance regulator Homo sapiens 0-4 23784542-0 2013 Carbachol-induced MUC17 endocytosis is concomitant with NHE3 internalization and CFTR membrane recruitment in enterocytes. Carbachol 0-9 solute carrier family 9 member A3 Homo sapiens 56-60 23784542-0 2013 Carbachol-induced MUC17 endocytosis is concomitant with NHE3 internalization and CFTR membrane recruitment in enterocytes. Carbachol 0-9 CF transmembrane conductance regulator Homo sapiens 81-85 23784542-3 2013 Carbachol (CCh) stimulation of the small intestine results in gel-forming mucin secretion from goblet cells, something that requires adjacent enterocytes to secrete chloride and bicarbonate for proper mucin formation. Carbachol 0-9 LOC100508689 Homo sapiens 74-79 23784542-3 2013 Carbachol (CCh) stimulation of the small intestine results in gel-forming mucin secretion from goblet cells, something that requires adjacent enterocytes to secrete chloride and bicarbonate for proper mucin formation. Carbachol 0-9 LOC100508689 Homo sapiens 201-206 23784542-3 2013 Carbachol (CCh) stimulation of the small intestine results in gel-forming mucin secretion from goblet cells, something that requires adjacent enterocytes to secrete chloride and bicarbonate for proper mucin formation. Carbachol 11-14 LOC100508689 Homo sapiens 74-79 23784542-3 2013 Carbachol (CCh) stimulation of the small intestine results in gel-forming mucin secretion from goblet cells, something that requires adjacent enterocytes to secrete chloride and bicarbonate for proper mucin formation. Carbachol 11-14 LOC100508689 Homo sapiens 201-206 23784542-4 2013 Surface labeling and confocal imaging demonstrated that apically expressed MUC17 in Caco-2 cells and Muc3(17) in murine enterocytes were endocytosed upon stimulation with CCh. Carbachol 171-174 mucin 17, cell surface associated Homo sapiens 75-80 23784542-4 2013 Surface labeling and confocal imaging demonstrated that apically expressed MUC17 in Caco-2 cells and Muc3(17) in murine enterocytes were endocytosed upon stimulation with CCh. Carbachol 171-174 MUC3 Homo sapiens 101-105 23784542-5 2013 Relocation of MUC17 in response to CCh was specific as MUC3 and MUC12 did not relocate following CCh stimulation. Carbachol 35-38 mucin 17, cell surface associated Homo sapiens 14-19 23784542-5 2013 Relocation of MUC17 in response to CCh was specific as MUC3 and MUC12 did not relocate following CCh stimulation. Carbachol 35-38 MUC3 Homo sapiens 55-59 23784542-5 2013 Relocation of MUC17 in response to CCh was specific as MUC3 and MUC12 did not relocate following CCh stimulation. Carbachol 35-38 mucin 12, cell surface associated Homo sapiens 64-69 23784542-5 2013 Relocation of MUC17 in response to CCh was specific as MUC3 and MUC12 did not relocate following CCh stimulation. Carbachol 97-100 mucin 17, cell surface associated Homo sapiens 14-19 23784542-6 2013 MUC17 colocalized with PDZK1 under basal conditions, while MUC17 relocated to the terminal web and into early endosomes after CCh stimulation. Carbachol 126-129 mucin 17, cell surface associated Homo sapiens 59-64 23784542-7 2013 CCh stimulation concomitantly internalized the Na(+/)H(+) exchanger 3 (NHE3) and recruited cystic fibrosis transmembrane conductance regulator (CFTR) to the apical membranes, a process that was important for CFTR-mediated bicarbonate secretion necessary for proper gel-forming mucin unfolding. Carbachol 0-3 solute carrier family 9 member A3 Homo sapiens 47-69 23784542-7 2013 CCh stimulation concomitantly internalized the Na(+/)H(+) exchanger 3 (NHE3) and recruited cystic fibrosis transmembrane conductance regulator (CFTR) to the apical membranes, a process that was important for CFTR-mediated bicarbonate secretion necessary for proper gel-forming mucin unfolding. Carbachol 0-3 solute carrier family 9 member A3 Homo sapiens 71-75 23784542-7 2013 CCh stimulation concomitantly internalized the Na(+/)H(+) exchanger 3 (NHE3) and recruited cystic fibrosis transmembrane conductance regulator (CFTR) to the apical membranes, a process that was important for CFTR-mediated bicarbonate secretion necessary for proper gel-forming mucin unfolding. Carbachol 0-3 CF transmembrane conductance regulator Homo sapiens 91-142 23784542-7 2013 CCh stimulation concomitantly internalized the Na(+/)H(+) exchanger 3 (NHE3) and recruited cystic fibrosis transmembrane conductance regulator (CFTR) to the apical membranes, a process that was important for CFTR-mediated bicarbonate secretion necessary for proper gel-forming mucin unfolding. Carbachol 0-3 CF transmembrane conductance regulator Homo sapiens 144-148 23784542-7 2013 CCh stimulation concomitantly internalized the Na(+/)H(+) exchanger 3 (NHE3) and recruited cystic fibrosis transmembrane conductance regulator (CFTR) to the apical membranes, a process that was important for CFTR-mediated bicarbonate secretion necessary for proper gel-forming mucin unfolding. Carbachol 0-3 CF transmembrane conductance regulator Homo sapiens 208-212 23784542-7 2013 CCh stimulation concomitantly internalized the Na(+/)H(+) exchanger 3 (NHE3) and recruited cystic fibrosis transmembrane conductance regulator (CFTR) to the apical membranes, a process that was important for CFTR-mediated bicarbonate secretion necessary for proper gel-forming mucin unfolding. Carbachol 0-3 LOC100508689 Homo sapiens 277-282 23816471-9 2013 Anti-beta-arrestin2 antibody partly blocked carbachol-induced increases of distal colonic strips in diabetic rats. Carbachol 44-53 arrestin, beta 2, pseudogene Rattus norvegicus 5-19 23703528-0 2013 PLC-gamma directly binds activated c-Src, which is necessary for carbachol-mediated inhibition of NHE3 activity in Caco-2/BBe cells. Carbachol 65-74 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 35-40 23703528-0 2013 PLC-gamma directly binds activated c-Src, which is necessary for carbachol-mediated inhibition of NHE3 activity in Caco-2/BBe cells. Carbachol 65-74 solute carrier family 9 member A3 Homo sapiens 98-102 23703528-5 2013 In Caco-2/BBe cells, carbachol (CCh) decreased NHE3 activity by ~40%, an effect abolished with the c-Src inhibitor PP2. Carbachol 21-30 solute carrier family 9 member A3 Homo sapiens 47-51 23703528-5 2013 In Caco-2/BBe cells, carbachol (CCh) decreased NHE3 activity by ~40%, an effect abolished with the c-Src inhibitor PP2. Carbachol 21-30 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 99-104 23703528-5 2013 In Caco-2/BBe cells, carbachol (CCh) decreased NHE3 activity by ~40%, an effect abolished with the c-Src inhibitor PP2. Carbachol 21-30 neuropeptide Y receptor Y6 (pseudogene) Homo sapiens 115-118 23703528-5 2013 In Caco-2/BBe cells, carbachol (CCh) decreased NHE3 activity by ~40%, an effect abolished with the c-Src inhibitor PP2. Carbachol 32-35 solute carrier family 9 member A3 Homo sapiens 47-51 23703528-5 2013 In Caco-2/BBe cells, carbachol (CCh) decreased NHE3 activity by ~40%, an effect abolished with the c-Src inhibitor PP2. Carbachol 32-35 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 99-104 23703528-5 2013 In Caco-2/BBe cells, carbachol (CCh) decreased NHE3 activity by ~40%, an effect abolished with the c-Src inhibitor PP2. Carbachol 32-35 neuropeptide Y receptor Y6 (pseudogene) Homo sapiens 115-118 23703528-6 2013 CCh treatment increased the amount of active c-Src as early as 1 min through increased Y(416) phosphorylation. Carbachol 0-3 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 45-50 23884641-6 2013 Functionally, the contractile EBs displayed calcium cycling and were responsive to the chronotropic agents isoprenaline (0.1 muM) and carbachol (1 muM). Carbachol 134-143 latexin Homo sapiens 147-150 23784544-6 2013 Carbachol (CCh) stimulated phosphatidylinositol (PI) hydrolysis, Rho kinase and zipper-interacting protein (ZIP) kinase activity, induced myosin phosphatase 1 (MYPT1) phosphorylation (at Thr(696)) and 20-kDa myosin light chain (MLC20) phosphorylation (at Ser(19)) and muscle contraction, and inhibited cAMP formation. Carbachol 0-9 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 65-75 23703528-7 2013 Coimmunoprecipitation demonstrated that c-Src associated with PLC-gamma, but not NHE3, under basal conditions, an interaction that increased rapidly after CCh treatment and occurred before the dissociation of PLC-gamma and NHE3 that occurred 10 min after CCh treatment. Carbachol 155-158 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 40-45 23703528-7 2013 Coimmunoprecipitation demonstrated that c-Src associated with PLC-gamma, but not NHE3, under basal conditions, an interaction that increased rapidly after CCh treatment and occurred before the dissociation of PLC-gamma and NHE3 that occurred 10 min after CCh treatment. Carbachol 255-258 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 40-45 23703528-9 2013 This study demonstrated that c-Src 1) activity is necessary for [Ca(2+)]i inhibition of NHE3 activity, 2) activation occurs rapidly (~1 min) after CCh treatment, 3) directly binds PLC-gamma SH2 domains and associates dynamically with PLC-gamma under elevated [Ca(2+)]i conditions, and 4) does not directly bind NHE3. Carbachol 147-150 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 29-34 23703528-9 2013 This study demonstrated that c-Src 1) activity is necessary for [Ca(2+)]i inhibition of NHE3 activity, 2) activation occurs rapidly (~1 min) after CCh treatment, 3) directly binds PLC-gamma SH2 domains and associates dynamically with PLC-gamma under elevated [Ca(2+)]i conditions, and 4) does not directly bind NHE3. Carbachol 147-150 solute carrier family 9 member A3 Homo sapiens 88-92 23784544-6 2013 Carbachol (CCh) stimulated phosphatidylinositol (PI) hydrolysis, Rho kinase and zipper-interacting protein (ZIP) kinase activity, induced myosin phosphatase 1 (MYPT1) phosphorylation (at Thr(696)) and 20-kDa myosin light chain (MLC20) phosphorylation (at Ser(19)) and muscle contraction, and inhibited cAMP formation. Carbachol 0-9 protein phosphatase 1, regulatory subunit 12A Mus musculus 138-158 23784544-7 2013 Stimulation of PI hydrolysis, Rho kinase, and ZIP kinase activity, phosphorylation of MYPT1 and MLC20, and muscle contraction in response to CCh were attenuated by methyl beta-cyclodextrin (MbetaCD) or caveolin-1 small interfering RNA (siRNA). Carbachol 141-144 protein phosphatase 1, regulatory subunit 12A Mus musculus 86-91 23784544-6 2013 Carbachol (CCh) stimulated phosphatidylinositol (PI) hydrolysis, Rho kinase and zipper-interacting protein (ZIP) kinase activity, induced myosin phosphatase 1 (MYPT1) phosphorylation (at Thr(696)) and 20-kDa myosin light chain (MLC20) phosphorylation (at Ser(19)) and muscle contraction, and inhibited cAMP formation. Carbachol 0-9 protein phosphatase 1, regulatory subunit 12A Mus musculus 160-165 23784544-6 2013 Carbachol (CCh) stimulated phosphatidylinositol (PI) hydrolysis, Rho kinase and zipper-interacting protein (ZIP) kinase activity, induced myosin phosphatase 1 (MYPT1) phosphorylation (at Thr(696)) and 20-kDa myosin light chain (MLC20) phosphorylation (at Ser(19)) and muscle contraction, and inhibited cAMP formation. Carbachol 0-9 myosin, light polypeptide 9, regulatory Mus musculus 228-233 23784544-7 2013 Stimulation of PI hydrolysis, Rho kinase, and ZIP kinase activity, phosphorylation of MYPT1 and MLC20, and muscle contraction in response to CCh were attenuated by methyl beta-cyclodextrin (MbetaCD) or caveolin-1 small interfering RNA (siRNA). Carbachol 141-144 myosin, light polypeptide 9, regulatory Mus musculus 96-101 23784544-7 2013 Stimulation of PI hydrolysis, Rho kinase, and ZIP kinase activity, phosphorylation of MYPT1 and MLC20, and muscle contraction in response to CCh were attenuated by methyl beta-cyclodextrin (MbetaCD) or caveolin-1 small interfering RNA (siRNA). Carbachol 141-144 caveolin 1, caveolae protein Mus musculus 202-212 23784544-8 2013 Similar inhibition of PI hydrolysis, Rho kinase, and ZIP kinase activity and muscle contraction in response to CCh and gastric emptying in vivo was obtained in caveolin-1-knockout mice compared with wild-type mice. Carbachol 111-114 death-associated protein kinase 3 Mus musculus 53-63 23784544-8 2013 Similar inhibition of PI hydrolysis, Rho kinase, and ZIP kinase activity and muscle contraction in response to CCh and gastric emptying in vivo was obtained in caveolin-1-knockout mice compared with wild-type mice. Carbachol 111-114 caveolin 1, caveolae protein Mus musculus 160-170 23786223-5 2013 The carbachol response was unaffected in the M2KO strain but decreased 42% in M3KO mice (p < 0.01). Carbachol 4-13 cholinergic receptor, muscarinic 3, cardiac Mus musculus 78-82 23475395-0 2013 Histamine, carbachol, and serotonin induce hyperresponsiveness to ATP in guinea pig tracheas: involvement of COX-2 pathway. Carbachol 11-20 cytochrome c oxidase subunit II Cavia porcellus 109-114 23475395-8 2013 Airway epithelial cells showed increased COX-2 mRNA after stimulation with histamine or carbachol, but not serotonin, while COX-1 mRNA was unaffected. Carbachol 88-97 cytochrome c oxidase subunit II Cavia porcellus 41-46 23475395-10 2013 In conclusion, we showed for the first time that histamine and carbachol cause hyperresponsiveness to ATP by upregulating COX-2 in airway epithelium, which likely increases TXA2 production. Carbachol 63-72 cytochrome c oxidase subunit II Cavia porcellus 122-127 23361868-7 2013 Carbamylcholine chloride, a parasympathomimetic drug, equally effectively reduced the heart rates of wild-type and EAAT3 knockout mice. Carbachol 0-24 solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 Mus musculus 115-120 23784544-6 2013 Carbachol (CCh) stimulated phosphatidylinositol (PI) hydrolysis, Rho kinase and zipper-interacting protein (ZIP) kinase activity, induced myosin phosphatase 1 (MYPT1) phosphorylation (at Thr(696)) and 20-kDa myosin light chain (MLC20) phosphorylation (at Ser(19)) and muscle contraction, and inhibited cAMP formation. Carbachol 11-14 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 65-75 23784544-6 2013 Carbachol (CCh) stimulated phosphatidylinositol (PI) hydrolysis, Rho kinase and zipper-interacting protein (ZIP) kinase activity, induced myosin phosphatase 1 (MYPT1) phosphorylation (at Thr(696)) and 20-kDa myosin light chain (MLC20) phosphorylation (at Ser(19)) and muscle contraction, and inhibited cAMP formation. Carbachol 11-14 protein phosphatase 1, regulatory subunit 12A Mus musculus 138-158 23784544-6 2013 Carbachol (CCh) stimulated phosphatidylinositol (PI) hydrolysis, Rho kinase and zipper-interacting protein (ZIP) kinase activity, induced myosin phosphatase 1 (MYPT1) phosphorylation (at Thr(696)) and 20-kDa myosin light chain (MLC20) phosphorylation (at Ser(19)) and muscle contraction, and inhibited cAMP formation. Carbachol 11-14 protein phosphatase 1, regulatory subunit 12A Mus musculus 160-165 23784544-6 2013 Carbachol (CCh) stimulated phosphatidylinositol (PI) hydrolysis, Rho kinase and zipper-interacting protein (ZIP) kinase activity, induced myosin phosphatase 1 (MYPT1) phosphorylation (at Thr(696)) and 20-kDa myosin light chain (MLC20) phosphorylation (at Ser(19)) and muscle contraction, and inhibited cAMP formation. Carbachol 11-14 myosin, light polypeptide 9, regulatory Mus musculus 228-233 23760269-4 2013 In cells expressing the M3 muscarinic acetylcholine receptor, the agonist carbachol (Cch) caused strong activation of CFTR through two pathways; the canonical PKA-dependent mechanism and a second mechanism that involves tyrosine phosphorylation. Carbachol 74-83 cystic fibrosis transmembrane conductance regulator Mesocricetus auratus 118-122 23760269-4 2013 In cells expressing the M3 muscarinic acetylcholine receptor, the agonist carbachol (Cch) caused strong activation of CFTR through two pathways; the canonical PKA-dependent mechanism and a second mechanism that involves tyrosine phosphorylation. Carbachol 85-88 cystic fibrosis transmembrane conductance regulator Mesocricetus auratus 118-122 23760269-6 2013 The role of tyrosine kinases was suggested by Cch stimulation of 15SA-CFTR and 9CA-CFTR, mutants that lack 15 PKA or 9 PKC consensus sequences and are unresponsive to PKA or PKC stimulation, respectively. Carbachol 46-49 cystic fibrosis transmembrane conductance regulator Mesocricetus auratus 70-74 23760269-6 2013 The role of tyrosine kinases was suggested by Cch stimulation of 15SA-CFTR and 9CA-CFTR, mutants that lack 15 PKA or 9 PKC consensus sequences and are unresponsive to PKA or PKC stimulation, respectively. Carbachol 46-49 cystic fibrosis transmembrane conductance regulator Mesocricetus auratus 83-87 23894286-4 2013 Using phage display screening, we have previously identified a heptapeptide, termed IQ, homologous to most nAChR subtypes, binding with nanomolar affinity to soluble Abeta40 and blocking Abeta-induced inhibition of carbamylcholine-induced currents in PC12 cells expressing alpha7 nAChRs. Carbachol 215-230 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 107-112 23727356-8 2013 In CHO-M1 cells, M1-mAChR activation by carbachol resulted in Ca(2+) mobilization, ERK1/2 phosphorylation and a reduction in thymidine incorporation, all of which were completely inhibited by MT-7, indicating the involvement of surface M1-mAChRs. Carbachol 40-49 mitogen-activated protein kinase 3 Mus musculus 83-89 23732791-1 2013 Through microfluidic interrogation we analyzed real-time calcium responses of HEK293 cells stimulated with short pulses of the M3 muscarinic receptor ligand carbachol in two different concentration regimes. Carbachol 157-166 cholinergic receptor muscarinic 3 Homo sapiens 127-149 23583240-11 2013 RGS4 knockout mice showed significantly enhanced sensitivity to AF induction in the presence of carbachol. Carbachol 96-105 regulator of G-protein signaling 4 Mus musculus 0-4 23639814-7 2013 As opposed to the lack of strain differences in the U46619-induced force, the newborn hph-1 gastric muscle carbachol-induced contraction and nNOS-dependent relaxation were significantly reduced (P < 0.01). Carbachol 107-116 hyperphenylalaninemia 1 Mus musculus 86-91 23685950-7 2013 Pretreatment with carbachol (0.01 mumol/L) blocked glutamate-induced cell death and GSK-3beta overactivation, and markedly enhanced beta-catenin transcriptional activity. Carbachol 18-27 glycogen synthase kinase 3 beta Rattus norvegicus 84-93 23685950-7 2013 Pretreatment with carbachol (0.01 mumol/L) blocked glutamate-induced cell death and GSK-3beta overactivation, and markedly enhanced beta-catenin transcriptional activity. Carbachol 18-27 catenin beta 1 Rattus norvegicus 132-144 23826977-6 2013 Strips of denuded detrusor or mucosa were mounted in organ baths to study the effect of TRPM8 agonists on the contractile responses to 10 mumol/L carbachol. Carbachol 146-155 transient receptor potential cation channel subfamily M member 8 Sus scrofa 88-93 23721928-6 2013 In both exposed and control animals, 100 muM carbachol had a transient excitatory effect on spontaneous activity followed by a rapid weakening of activity to near or below normal levels. Carbachol 45-54 latexin Homo sapiens 41-44 23613531-4 2013 CCh increased MYPT1 phosphorylation at Thr696 (pT696) and Thr853 (pT853), CPI-17 at Thr38 (pT38), and myosin light chain at Ser19 (pS19). Carbachol 0-3 protein phosphatase 1, regulatory subunit 12A Mus musculus 14-19 23613531-4 2013 CCh increased MYPT1 phosphorylation at Thr696 (pT696) and Thr853 (pT853), CPI-17 at Thr38 (pT38), and myosin light chain at Ser19 (pS19). Carbachol 0-3 protein phosphatase 1, regulatory inhibitor subunit 14A Mus musculus 74-80 23613531-12 2013 Bath-applied CCh recruits additional ROCK-dependent MYPT1 phosphorylation due to exposure of the agonist to a wider population of muscarinic receptors. Carbachol 13-16 protein phosphatase 1, regulatory subunit 12A Mus musculus 52-57 23529130-2 2013 Induction of currents (ICCh) in TRPC6-expressing HEK293 cells by a muscarinic agonist carbachol (CCh; 100 mum) was strongly attenuated by a CaMKII-specific peptide, autocamtide-2-related inhibitory peptide (AIP; 10 mum). Carbachol 86-95 transient receptor potential cation channel subfamily C member 6 Homo sapiens 32-37 23651161-5 2013 In this study, using in vitro whole-cell patch-clamp recordings in a rat hippocampal slice preparation, we show that hippocampal CA3 pyramidal cells support persistent firing under perfusion of the cholinergic agonist carbachol (10 mum). Carbachol 218-227 carbonic anhydrase 3 Rattus norvegicus 129-132 23529130-2 2013 Induction of currents (ICCh) in TRPC6-expressing HEK293 cells by a muscarinic agonist carbachol (CCh; 100 mum) was strongly attenuated by a CaMKII-specific peptide, autocamtide-2-related inhibitory peptide (AIP; 10 mum). Carbachol 24-27 transient receptor potential cation channel subfamily C member 6 Homo sapiens 32-37 23529130-2 2013 Induction of currents (ICCh) in TRPC6-expressing HEK293 cells by a muscarinic agonist carbachol (CCh; 100 mum) was strongly attenuated by a CaMKII-specific peptide, autocamtide-2-related inhibitory peptide (AIP; 10 mum). Carbachol 86-95 calcium/calmodulin dependent protein kinase II gamma Homo sapiens 140-146 23529130-2 2013 Induction of currents (ICCh) in TRPC6-expressing HEK293 cells by a muscarinic agonist carbachol (CCh; 100 mum) was strongly attenuated by a CaMKII-specific peptide, autocamtide-2-related inhibitory peptide (AIP; 10 mum). Carbachol 24-27 calcium/calmodulin dependent protein kinase II gamma Homo sapiens 140-146 23608222-8 2013 Substantial glucose-induced insulin secretion was induced in the pancreas perfusion study of Kir6.2(-/-) mice only in the presence of carbamylcholine. Carbachol 134-149 potassium inwardly rectifying channel, subfamily J, member 11 Mus musculus 93-99 23525004-1 2013 Cholesterol depletion reversibly abolishes carbachol-evoked Ca(2+) release from inositol (1,4,5)-trisphosphate (IP3)-sensitive stores, without affecting the distribution of IP3 receptors (IP3R) or endoplasmic reticulum, IP3 formation or responses to photolysis of caged IP3. Carbachol 43-52 inositol 1,4,5-trisphosphate receptor type 3 Homo sapiens 188-192 23546599-8 2013 In organ bath study using bladder strips, the PTHrP peptide caused a marked reduction in the amplitude of spontaneous contraction but caused only modest suppression for carbachol-induced contraction. Carbachol 169-178 parathyroid hormone-like hormone Rattus norvegicus 46-51 23513060-4 2013 RESULTS: Exposure to IL-6 at concentrations of 1 and 10 ng/mL and IL-1beta at 10 ng/mL significantly decreased CCh-induced Cl(-) secretion. Carbachol 111-114 interleukin 6 Homo sapiens 21-25 23353793-0 2013 Involvement of the Tyr kinase/JNK pathway in carbachol-induced bronchial smooth muscle contraction in the rat. Carbachol 45-54 mitogen-activated protein kinase 8 Rattus norvegicus 30-33 23353793-7 2013 The contraction induced by carbachol (CCh) was significantly inhibited by pretreatment with selective Tyr kinase inhibitors (genistein and ST638, n = 6, respectively), and a JNK inhibitor (SP600125, n = 6). Carbachol 27-36 mitogen-activated protein kinase 8 Rattus norvegicus 174-177 23353793-7 2013 The contraction induced by carbachol (CCh) was significantly inhibited by pretreatment with selective Tyr kinase inhibitors (genistein and ST638, n = 6, respectively), and a JNK inhibitor (SP600125, n = 6). Carbachol 38-41 mitogen-activated protein kinase 8 Rattus norvegicus 174-177 23353793-11 2013 The JNK phosphorylation induced by CCh was significantly inhibited by SP600125 (n = 4). Carbachol 35-38 mitogen-activated protein kinase 8 Rattus norvegicus 4-7 23945308-9 2013 With 20 mmol/L caffeine and 100 micromol/L carbachol which stimulated Ca2+ release respectively from internal ryanodine receptor (RYR) and inositol triphosphate (IP3) Ca2+ storage, the fluorescence intensity F/F0 curve presented a peak pattern. Carbachol 43-52 ryanodine receptor 2 Rattus norvegicus 130-133 23513060-4 2013 RESULTS: Exposure to IL-6 at concentrations of 1 and 10 ng/mL and IL-1beta at 10 ng/mL significantly decreased CCh-induced Cl(-) secretion. Carbachol 111-114 interleukin 1 beta Homo sapiens 66-74 23513060-6 2013 CCh, 10 muM, prevented CCh, 100 muM, from eliciting Cl(-) secretion. Carbachol 0-3 latexin Homo sapiens 8-11 23339174-9 2013 Carbachol-stimulated acid secretion was higher in GFRalpha2-KO mice, while atropine reduced basal secretion similarly in both genotypes. Carbachol 0-9 glial cell line derived neurotrophic factor family receptor alpha 2 Mus musculus 50-59 23513060-6 2013 CCh, 10 muM, prevented CCh, 100 muM, from eliciting Cl(-) secretion. Carbachol 0-3 latexin Homo sapiens 32-35 23513060-6 2013 CCh, 10 muM, prevented CCh, 100 muM, from eliciting Cl(-) secretion. Carbachol 23-26 latexin Homo sapiens 8-11 23513060-6 2013 CCh, 10 muM, prevented CCh, 100 muM, from eliciting Cl(-) secretion. Carbachol 23-26 latexin Homo sapiens 32-35 22022845-4 2013 RESULTS: Carbachol (CCh), an analog of acetylcholine (ACh) significantly enhanced de novo differentiation into neurons on bFGF- and EGF-deprived stem cells as shown by the percentage of TUJ1 positive cells. Carbachol 9-18 fibroblast growth factor 2 Mus musculus 122-126 23365260-6 2013 Although carbachol (CCh)-induced (0.1-100 microM) time- and dose-dependent contractions in Adip-Sen mouse bladder were slightly enhanced, compared with those in the C57Bl mouse during a low range (0.3-1.0 microM) of CCh, differences could not be detected with other CCh concentrations. Carbachol 9-18 synovial sarcoma, X 2 interacting protein Mus musculus 91-95 23365260-6 2013 Although carbachol (CCh)-induced (0.1-100 microM) time- and dose-dependent contractions in Adip-Sen mouse bladder were slightly enhanced, compared with those in the C57Bl mouse during a low range (0.3-1.0 microM) of CCh, differences could not be detected with other CCh concentrations. Carbachol 20-23 synovial sarcoma, X 2 interacting protein Mus musculus 91-95 23365260-6 2013 Although carbachol (CCh)-induced (0.1-100 microM) time- and dose-dependent contractions in Adip-Sen mouse bladder were slightly enhanced, compared with those in the C57Bl mouse during a low range (0.3-1.0 microM) of CCh, differences could not be detected with other CCh concentrations. Carbachol 216-219 synovial sarcoma, X 2 interacting protein Mus musculus 91-95 23365260-6 2013 Although carbachol (CCh)-induced (0.1-100 microM) time- and dose-dependent contractions in Adip-Sen mouse bladder were slightly enhanced, compared with those in the C57Bl mouse during a low range (0.3-1.0 microM) of CCh, differences could not be detected with other CCh concentrations. Carbachol 216-219 synovial sarcoma, X 2 interacting protein Mus musculus 91-95 23365260-10 2013 Expression of the calcium-dependent isoform of PKC, PKCalpha, was increased in the Adip-Sen mouse bladder, and CCh-induced phosphorylation of PKCalpha was also enhanced, compared with those in the C57Bl mouse. Carbachol 111-114 protein kinase C, alpha Mus musculus 47-50 23365260-10 2013 Expression of the calcium-dependent isoform of PKC, PKCalpha, was increased in the Adip-Sen mouse bladder, and CCh-induced phosphorylation of PKCalpha was also enhanced, compared with those in the C57Bl mouse. Carbachol 111-114 synovial sarcoma, X 2 interacting protein Mus musculus 83-87 23365260-10 2013 Expression of the calcium-dependent isoform of PKC, PKCalpha, was increased in the Adip-Sen mouse bladder, and CCh-induced phosphorylation of PKCalpha was also enhanced, compared with those in the C57Bl mouse. Carbachol 111-114 protein kinase C, alpha Mus musculus 142-150 22022845-4 2013 RESULTS: Carbachol (CCh), an analog of acetylcholine (ACh) significantly enhanced de novo differentiation into neurons on bFGF- and EGF-deprived stem cells as shown by the percentage of TUJ1 positive cells. Carbachol 20-23 fibroblast growth factor 2 Mus musculus 122-126 23047022-7 2013 Cellular release of AChE by SH-SY5Y is significantly enhanced by the muscarinic acetylcholine receptor (mAChR) agonists carbachol or muscarine, with the effect of carbachol blocked by the mAChR antagonist atropine. Carbachol 120-129 acetylcholinesterase Mus musculus 20-24 23047022-7 2013 Cellular release of AChE by SH-SY5Y is significantly enhanced by the muscarinic acetylcholine receptor (mAChR) agonists carbachol or muscarine, with the effect of carbachol blocked by the mAChR antagonist atropine. Carbachol 163-172 acetylcholinesterase Mus musculus 20-24 23292809-4 2013 We found that the muscarinic agonists, carbachol (CCh) and oxotremorine, activated ENaC in a dose-dependent manner but that nicotine did not. Carbachol 39-48 sodium channel epithelial 1 subunit gamma Rattus norvegicus 83-87 23292809-4 2013 We found that the muscarinic agonists, carbachol (CCh) and oxotremorine, activated ENaC in a dose-dependent manner but that nicotine did not. Carbachol 50-53 sodium channel epithelial 1 subunit gamma Rattus norvegicus 83-87 23292809-5 2013 CCh-induced activation of ENaC was blocked by atropine. Carbachol 0-3 sodium channel epithelial 1 subunit gamma Rattus norvegicus 26-30 23292809-7 2013 Endogenous RhoA and GTP-RhoA increased in response to CCh and the increase was reduced by pretreatment with atropine. Carbachol 54-57 ras homolog family member A Rattus norvegicus 11-15 23292809-7 2013 Endogenous RhoA and GTP-RhoA increased in response to CCh and the increase was reduced by pretreatment with atropine. Carbachol 54-57 ras homolog family member A Rattus norvegicus 24-28 23292809-8 2013 We showed that Y-27632, an inhibitor of Rho-associated protein kinase (ROCK), abolished endogenous ENaC activity and inhibited the activation of ENaC by CCh. Carbachol 153-156 sodium channel epithelial 1 subunit gamma Rattus norvegicus 145-149 23362260-4 2013 Phosphorylation of the myosin light chain phosphatase regulatory subunit MYPT1 at Thr-696 and Thr-853 and the inhibitor protein CPI-17 were also stimulated with carbachol. Carbachol 161-170 protein phosphatase 1 regulatory subunit 12A Homo sapiens 73-78 23362260-4 2013 Phosphorylation of the myosin light chain phosphatase regulatory subunit MYPT1 at Thr-696 and Thr-853 and the inhibitor protein CPI-17 were also stimulated with carbachol. Carbachol 161-170 protein phosphatase 1 regulatory inhibitor subunit 14A Homo sapiens 128-134 23275618-6 2013 Both OA and Epac ligand stimulated Ras-related protein 1 (Rap1) and inhibited carbachol (CCh)-induced Rho kinase activity. Carbachol 78-87 Rap guanine nucleotide exchange factor (GEF) 3 Mus musculus 12-16 23275618-6 2013 Both OA and Epac ligand stimulated Ras-related protein 1 (Rap1) and inhibited carbachol (CCh)-induced Rho kinase activity. Carbachol 89-92 Rap guanine nucleotide exchange factor (GEF) 3 Mus musculus 12-16 22865467-3 2012 The inhibition of the epidermal growth factor receptor (EGFR) by AG1478 or protein kinase C (PKC) by GF109203X significantly reduced carbachol-stimulated ERK1/2 activation and cell proliferation. Carbachol 133-142 epidermal growth factor receptor Homo sapiens 22-54 23007238-8 2013 RESULTS: T1N0Mx-IgG promoted tumor cell migration and increased MMP9 activity mimicking the action of the muscarinic agonist carbachol. Carbachol 125-134 matrix metallopeptidase 9 Homo sapiens 64-68 23246623-5 2013 Detrusor contractility was evaluated by organ bath studies and strips were incubated with carbachol (1muM) to induce and enhance tension. Carbachol 90-99 latexin Homo sapiens 102-105 23095462-8 2013 Relaxation to CCh was reduced in TRPC1 KO and TRPC3 KO aortas with concomitant reduction in EC Ca(2+) response. Carbachol 14-17 transient receptor potential cation channel, subfamily C, member 1 Mus musculus 33-38 23095462-8 2013 Relaxation to CCh was reduced in TRPC1 KO and TRPC3 KO aortas with concomitant reduction in EC Ca(2+) response. Carbachol 14-17 transient receptor potential cation channel, subfamily C, member 3 Mus musculus 46-51 23095462-9 2013 Pyr3 (TRPC3 blocker) reduced the Ca(2+) response to CCh in EC from WT, but not TRPC3 KO mice. Carbachol 52-55 transient receptor potential cation channel, subfamily C, member 3 Mus musculus 6-11 23090691-4 2013 Coprecipitating Gbeta with Kir3.1, detected by Western blotting, was significantly augmented by carbachol. Carbachol 96-105 potassium inwardly-rectifying channel, subfamily J, member 3 Mus musculus 27-33 23090691-5 2013 Atropine, but not tertiapin, significantly inhibited the carbachol-induced coprecipitating Gbeta with Kir3.1. Carbachol 57-66 potassium inwardly-rectifying channel, subfamily J, member 3 Mus musculus 102-108 23520542-6 2013 Voltage-clamp experiments using ramped voltage commands showed that CCh resulted in the gradual development of an inward current that was partially blocked by concurrent application of the selective Kv7.2/3 channel antagonist XE-991, which inhibits the muscarine-dependent K(+) current I M. The remaining inward current also reversed near EK and was inhibited by the K(+) channel blocker Ba(2+), suggesting that M1 receptor activation attenuates both I M as well as an additional K(+) current. Carbachol 68-71 potassium voltage-gated channel subfamily Q member 2 Homo sapiens 199-206 23460876-7 2013 We demonstrated that T1N0Mx-IgG (10(-8) M) and carbachol (10(-9) M) increased the constitutive expression of VEGF-A in tumor cells, effect that was reverted by the muscarinic antagonist atropine. Carbachol 47-56 vascular endothelial growth factor A Homo sapiens 109-115 23460876-10 2013 In conclusion, T1N0Mx-IgG and carbachol may promote VEGF-A production and neovascularization induced by breast tumor cells via muscarinic receptors activation. Carbachol 30-39 vascular endothelial growth factor A Homo sapiens 52-58 23469045-3 2013 In combination with carbachol (10 microM), rivastigmine (1 microM) significantly decreased the release of nitric oxide, TNF-alpha, IL-1beta and IL-6 from lipopolysaccharide-activated RAW 264.7 macrophages and this effect was abolished by alpha7 nicotinic receptor blockade by bungarotoxin. Carbachol 20-29 tumor necrosis factor Rattus norvegicus 120-129 23469045-3 2013 In combination with carbachol (10 microM), rivastigmine (1 microM) significantly decreased the release of nitric oxide, TNF-alpha, IL-1beta and IL-6 from lipopolysaccharide-activated RAW 264.7 macrophages and this effect was abolished by alpha7 nicotinic receptor blockade by bungarotoxin. Carbachol 20-29 interleukin 1 beta Rattus norvegicus 131-139 23469045-3 2013 In combination with carbachol (10 microM), rivastigmine (1 microM) significantly decreased the release of nitric oxide, TNF-alpha, IL-1beta and IL-6 from lipopolysaccharide-activated RAW 264.7 macrophages and this effect was abolished by alpha7 nicotinic receptor blockade by bungarotoxin. Carbachol 20-29 interleukin 6 Rattus norvegicus 144-148 23382834-5 2013 HSG-hAQP5 pre-incubated with SjS plasma for 24 hours significantly reduced AQP5 trafficking with CCh, compared with HSG-hAQP5 pre-incubated with healthy control (HC) plasma. Carbachol 97-100 aquaporin 5 Homo sapiens 4-9 23382834-5 2013 HSG-hAQP5 pre-incubated with SjS plasma for 24 hours significantly reduced AQP5 trafficking with CCh, compared with HSG-hAQP5 pre-incubated with healthy control (HC) plasma. Carbachol 97-100 aquaporin 5 Homo sapiens 5-9 22902499-5 2012 KEY FINDINGS: Both nAChR and mAChR antagonists (hexamethonium and methacine, respectively), per se, elevated histamine-releasing activity of the HMC-1 and suppressed the MC responses to most of investigated activators (carbachol, compound 48/80, and to a lesser extent aIgG). Carbachol 219-228 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 19-24 23098909-7 2012 Carbachol exerts its beneficial effect on the amelioration of the TJ damage by inhibiting the NF-kappabeta and MLCK pathways in an alpha7nAchR-dependent manner. Carbachol 0-9 myosin light chain kinase Rattus norvegicus 111-115 23397206-10 2013 A carbachol-induced increase in the cytosolic Ca(2+) concentration was significantly reduced when cells were pretreated with siRNA against STIM1. Carbachol 2-11 stromal interaction molecule 1 Rattus norvegicus 139-144 23431067-7 2013 Tyrosine phosphorylation of heterologously expressed NCKX2-WT, but not NCKX2-Y365A, was increased by carbachol (CCh) in PC-12 cells. Carbachol 101-110 solute carrier family 24 member 2 Rattus norvegicus 53-58 23431067-7 2013 Tyrosine phosphorylation of heterologously expressed NCKX2-WT, but not NCKX2-Y365A, was increased by carbachol (CCh) in PC-12 cells. Carbachol 112-115 solute carrier family 24 member 2 Rattus norvegicus 53-58 23122879-4 2013 The retrograde tracer cholera toxin subunit b (CTb) was administered in pontine regions where carbachol microinjections induced REM sleep. Carbachol 94-103 phosphate cytidylyltransferase 1B, choline Homo sapiens 22-45 23122879-4 2013 The retrograde tracer cholera toxin subunit b (CTb) was administered in pontine regions where carbachol microinjections induced REM sleep. Carbachol 94-103 phosphate cytidylyltransferase 1B, choline Homo sapiens 47-50 23135699-3 2013 Switching from SMB to SMA MHC protein expression decreased the rate of the force transient and increased the sustained tonic force in SMB((-/-)) ileum and antrum with high potassium (KPSS) but not with carbachol (CCh) stimulation. Carbachol 213-216 immunoglobulin mu binding protein 2 Mus musculus 22-25 23135699-6 2013 However, specifically activating PKCalpha with phorbol dibutyrate (PDBu) was not significantly different in knockout and wild-type tissues, with total force being a fraction of the force generation with KPSS or CCh stimulation in SMB((-/-)) ileum and antrum. Carbachol 211-214 small nuclear ribonucleoprotein B Mus musculus 230-233 23246623-0 2013 The novel beta3-adrenoceptor agonist mirabegron reduces carbachol-induced contractile activity in detrusor tissue from patients with bladder outflow obstruction with or without detrusor overactivity. Carbachol 56-65 adrenoceptor beta 3 Homo sapiens 10-28 23022458-7 2013 Furthermore, moderate treatment of Slc10a4 null slices with the cholinergic agonist carbachol induced epileptiform activity. Carbachol 84-93 solute carrier family 10 (sodium/bile acid cotransporter family), member 4 Mus musculus 35-42 22861176-5 2013 When evaluated for inhibition of the carbachol-induced contraction of rat urinary bladder, 5-HMT MS showed a much longer and more potent effect than tolterodine tablets. Carbachol 37-46 histamine N-methyltransferase Rattus norvegicus 93-96 23098909-0 2012 Carbachol ameliorates lipopolysaccharide-induced intestinal epithelial tight junction damage by down-regulating NF-kappabeta and myosin light-chain kinase pathways. Carbachol 0-9 myosin light chain kinase Rattus norvegicus 129-154 22906005-3 2012 A double phosphorylation-deficient mutant (S490/543A) of AC2 was insensitive to PMA (phorbol myristic acid) and CCh (carbachol) stimulation, whereas a double phosphomimetic mutant (S490/543D) mimicked the activity of PKC-activated AC2. Carbachol 112-115 adenylate cyclase 2 Homo sapiens 57-60 22906005-3 2012 A double phosphorylation-deficient mutant (S490/543A) of AC2 was insensitive to PMA (phorbol myristic acid) and CCh (carbachol) stimulation, whereas a double phosphomimetic mutant (S490/543D) mimicked the activity of PKC-activated AC2. Carbachol 117-126 adenylate cyclase 2 Homo sapiens 57-60 22865467-3 2012 The inhibition of the epidermal growth factor receptor (EGFR) by AG1478 or protein kinase C (PKC) by GF109203X significantly reduced carbachol-stimulated ERK1/2 activation and cell proliferation. Carbachol 133-142 epidermal growth factor receptor Homo sapiens 56-60 22865467-3 2012 The inhibition of the epidermal growth factor receptor (EGFR) by AG1478 or protein kinase C (PKC) by GF109203X significantly reduced carbachol-stimulated ERK1/2 activation and cell proliferation. Carbachol 133-142 mitogen-activated protein kinase 3 Homo sapiens 154-160 22865467-4 2012 Cotreatment of the cells with AG1478 and GF109203X produced an additive effect on carbachol-stimulated ERK1/2 activation, suggesting that the EGFR and PKC pathways act in parallel. Carbachol 82-91 mitogen-activated protein kinase 3 Homo sapiens 103-109 22865467-4 2012 Cotreatment of the cells with AG1478 and GF109203X produced an additive effect on carbachol-stimulated ERK1/2 activation, suggesting that the EGFR and PKC pathways act in parallel. Carbachol 82-91 epidermal growth factor receptor Homo sapiens 142-146 22865467-6 2012 In SNU-407 cells, carbachol treatment induced RSK activation in an atropine-sensitive manner, and this RSK activation was decreased by the inhibition of either EGFR or PKC. Carbachol 18-27 ribosomal protein S6 kinase A1 Homo sapiens 46-49 22865467-6 2012 In SNU-407 cells, carbachol treatment induced RSK activation in an atropine-sensitive manner, and this RSK activation was decreased by the inhibition of either EGFR or PKC. Carbachol 18-27 ribosomal protein S6 kinase A1 Homo sapiens 103-106 22865467-6 2012 In SNU-407 cells, carbachol treatment induced RSK activation in an atropine-sensitive manner, and this RSK activation was decreased by the inhibition of either EGFR or PKC. Carbachol 18-27 epidermal growth factor receptor Homo sapiens 160-164 22865467-7 2012 Moreover, the RSK-specific inhibitor BRD7389 almost completely blocked carbachol-stimulated cell proliferation. Carbachol 71-80 ribosomal protein S6 kinase A1 Homo sapiens 14-17 22936272-8 2012 Both cAMP and carbachol recruited NKCC1 to the basolateral membrane of enterocytes, while luminal acid or HCO(3)(-) retained NKCC1 in intracellular vesicles. Carbachol 14-23 solute carrier family 12 member 2 Rattus norvegicus 34-39 22814700-3 2012 In addition, the effect of colitis on the possible involvement of NCX-1 in the reduced carbachol-induced contraction of the rat colon was examined. Carbachol 87-96 solute carrier family 8 member A1 Rattus norvegicus 66-71 22814700-13 2012 Functional experiments demonstrated that NCX in reverse mode played a role in carbachol-induced contraction of colon, and this was not affected by colitis. Carbachol 78-87 solute carrier family 8 member A1 Rattus norvegicus 41-44 23077334-8 2012 Our results indicate that a protocol which yields STP (5 Hz, 5 sec) when paired with coapplication of the beta-adrenergic agonist, isoproterenol (ISO), and the cholinergic agonist, carbachol (CCh), induces translation-dependent LTP in mouse CA1. Carbachol 181-190 sulfotransferase family 1A, phenol-preferring, member 1 Mus musculus 50-53 22962328-4 2012 METHODS AND RESULTS: We demonstrate that IL-1beta-conditioned medium from RAW 264.7 macrophages induces differentiation of human adipose tissue-derived mesenchymal stem cells to alpha-smooth muscle actin-positive SMCs, and the differentiated SMCs exhibited increased contractility in response to KCl and carbachol treatment. Carbachol 304-313 interleukin 1 beta Homo sapiens 41-49 22859298-3 2012 In this study we identify Raf-1 kinase inhibitory protein as an essential mediator of ethanol-induced sensitization of cholecystokinin- and carbachol-regulated Ca(2+) signaling in pancreatic acinar cells. Carbachol 140-149 phosphatidylethanolamine binding protein 1 Homo sapiens 26-57 22903161-0 2012 Roles of AQP5/AQP5-G103D in carbamylcholine-induced volume decrease and in reduction of the activation energy for water transport by rat parotid acinar cells. Carbachol 28-43 aquaporin 5 Rattus norvegicus 9-13 22903161-0 2012 Roles of AQP5/AQP5-G103D in carbamylcholine-induced volume decrease and in reduction of the activation energy for water transport by rat parotid acinar cells. Carbachol 28-43 aquaporin 5 Rattus norvegicus 14-18 22859298-5 2012 Furthermore, we show that either suppression of Raf-1 kinase inhibitory protein expression using short hairpin RNA or gene ablation prevented the sensitizing effects of ethanol on cholecystokinin- and carbachol-stimulated Ca(2+) signaling and intracellular chymotrypsin activation in pancreatic acinar cells, suggesting that the modulation of Raf-1 inhibitory protein expression may have future therapeutic utility in the prevention or treatment of alcohol-associated pancreatitis. Carbachol 201-210 phosphatidylethanolamine binding protein 1 Homo sapiens 48-79 22859298-5 2012 Furthermore, we show that either suppression of Raf-1 kinase inhibitory protein expression using short hairpin RNA or gene ablation prevented the sensitizing effects of ethanol on cholecystokinin- and carbachol-stimulated Ca(2+) signaling and intracellular chymotrypsin activation in pancreatic acinar cells, suggesting that the modulation of Raf-1 inhibitory protein expression may have future therapeutic utility in the prevention or treatment of alcohol-associated pancreatitis. Carbachol 201-210 Raf-1 proto-oncogene, serine/threonine kinase Homo sapiens 48-53 22644105-1 2012 This study aimed to characterize the beta-adrenoceptor (beta-AR) subtype mediating relaxation of isolated human bladder strips and to explore relaxation by the novel beta3-AR-selective agonist KUC-7322 for its relaxant effect on the human isolated detrusor and for its effect on the carbachol (CCh)-induced contractile response. Carbachol 283-292 adrenoceptor beta 3 Homo sapiens 166-174 22975404-11 2012 Cch-stimulated increase in [Ca(2+)](i) was blocked by inhibitors for the M(1)AchRs, matrix metalloproteinases, and EGF receptors. Carbachol 0-3 epidermal growth factor like 1 Rattus norvegicus 115-118 22975404-12 2012 Inhibitors against the EGF receptor and ERK 1/2 also blocked Cch-stimulated mucin secretion. Carbachol 61-64 epidermal growth factor receptor Rattus norvegicus 23-35 22975404-12 2012 Inhibitors against the EGF receptor and ERK 1/2 also blocked Cch-stimulated mucin secretion. Carbachol 61-64 mitogen activated protein kinase 3 Rattus norvegicus 40-47 22975404-12 2012 Inhibitors against the EGF receptor and ERK 1/2 also blocked Cch-stimulated mucin secretion. Carbachol 61-64 solute carrier family 13 member 2 Rattus norvegicus 76-81 22430195-1 2012 The relaxant effect of an aryloxypropanolamine beta3-adrenoceptor agonist on carbachol pre-contracted human detrusor muscle strips was evaluated and compared with literature results from reference compounds of similar mode of action, including mirabegron. Carbachol 77-86 adrenoceptor beta 3 Homo sapiens 47-65 22115428-8 2012 Bladder strips from Eln(+/-) -sham mice showed a significantly heightened contractile response to both EFS and carbachol compared with Wt-sham mice. Carbachol 111-120 elastin Mus musculus 20-28 22115428-10 2012 CONCLUSION: The results that elastin-deficient mice had decreased bladder compliance and capacity and increased bladder contractility; and that Wt-pBOO mice showed an enhanced contractile response to carbachol, but Eln(+/-) -pBOO mice did not, suggest that elastin is critical for normal bladder function and is involved in bladder response to pBOO. Carbachol 202-211 elastin Mus musculus 259-266 22644105-1 2012 This study aimed to characterize the beta-adrenoceptor (beta-AR) subtype mediating relaxation of isolated human bladder strips and to explore relaxation by the novel beta3-AR-selective agonist KUC-7322 for its relaxant effect on the human isolated detrusor and for its effect on the carbachol (CCh)-induced contractile response. Carbachol 294-297 adrenoceptor beta 3 Homo sapiens 166-174 25279100-6 2012 Intracellular calcium level measurements in transfected cells revealed a more intensive carbachol induced increase of calcium concentration in HEK 293 cells expressing TRPC6P112Q versus the cells expressing wild-type TRPC6. Carbachol 88-97 TRPC6 pseudogene 1 Homo sapiens 168-174 21878485-5 2012 Hippocampal slice preparations from NRG1(tg-type I) mice exhibited a reduced frequency of carbachol-induced gamma oscillations and an increased tendency to epileptiform activity. Carbachol 90-99 neuregulin 1 Mus musculus 36-40 22683571-10 2012 In human lung slices, chronic beta-agonist exposure culminated in 64 +- 5.7% (P < 0.001) desensitization of beta(2)AR-mediated dilation of carbachol-constricted airways that was reversed by chloroquine. Carbachol 142-151 adrenoceptor beta 2 Homo sapiens 111-120 25279100-6 2012 Intracellular calcium level measurements in transfected cells revealed a more intensive carbachol induced increase of calcium concentration in HEK 293 cells expressing TRPC6P112Q versus the cells expressing wild-type TRPC6. Carbachol 88-97 transient receptor potential cation channel subfamily C member 6 Homo sapiens 168-173 22511543-5 2012 Carbachol (CCh), a muscarinic receptor-specific agonist, abrogated tumor necrosis factor alpha/interferon gamma-induced apoptosis through pathways involving caspase 3/7, but its cytoprotective effect was decreased by a M3R antagonist, a mitogen-activated protein kinase kinase/extracellular signal-regulated kinase (ERK) inhibitor, a phosphatidylinositol 3-kinase/Akt inhibitor, or an epidermal growth factor receptor (EGFR) inhibitor. Carbachol 0-9 caspase 3 Homo sapiens 157-166 22511543-5 2012 Carbachol (CCh), a muscarinic receptor-specific agonist, abrogated tumor necrosis factor alpha/interferon gamma-induced apoptosis through pathways involving caspase 3/7, but its cytoprotective effect was decreased by a M3R antagonist, a mitogen-activated protein kinase kinase/extracellular signal-regulated kinase (ERK) inhibitor, a phosphatidylinositol 3-kinase/Akt inhibitor, or an epidermal growth factor receptor (EGFR) inhibitor. Carbachol 0-9 mitogen-activated protein kinase 1 Homo sapiens 316-319 22511543-5 2012 Carbachol (CCh), a muscarinic receptor-specific agonist, abrogated tumor necrosis factor alpha/interferon gamma-induced apoptosis through pathways involving caspase 3/7, but its cytoprotective effect was decreased by a M3R antagonist, a mitogen-activated protein kinase kinase/extracellular signal-regulated kinase (ERK) inhibitor, a phosphatidylinositol 3-kinase/Akt inhibitor, or an epidermal growth factor receptor (EGFR) inhibitor. Carbachol 0-9 AKT serine/threonine kinase 1 Homo sapiens 364-367 22511543-5 2012 Carbachol (CCh), a muscarinic receptor-specific agonist, abrogated tumor necrosis factor alpha/interferon gamma-induced apoptosis through pathways involving caspase 3/7, but its cytoprotective effect was decreased by a M3R antagonist, a mitogen-activated protein kinase kinase/extracellular signal-regulated kinase (ERK) inhibitor, a phosphatidylinositol 3-kinase/Akt inhibitor, or an epidermal growth factor receptor (EGFR) inhibitor. Carbachol 0-9 epidermal growth factor receptor Homo sapiens 385-417 22511543-5 2012 Carbachol (CCh), a muscarinic receptor-specific agonist, abrogated tumor necrosis factor alpha/interferon gamma-induced apoptosis through pathways involving caspase 3/7, but its cytoprotective effect was decreased by a M3R antagonist, a mitogen-activated protein kinase kinase/extracellular signal-regulated kinase (ERK) inhibitor, a phosphatidylinositol 3-kinase/Akt inhibitor, or an epidermal growth factor receptor (EGFR) inhibitor. Carbachol 0-9 epidermal growth factor receptor Homo sapiens 419-423 22511543-5 2012 Carbachol (CCh), a muscarinic receptor-specific agonist, abrogated tumor necrosis factor alpha/interferon gamma-induced apoptosis through pathways involving caspase 3/7, but its cytoprotective effect was decreased by a M3R antagonist, a mitogen-activated protein kinase kinase/extracellular signal-regulated kinase (ERK) inhibitor, a phosphatidylinositol 3-kinase/Akt inhibitor, or an epidermal growth factor receptor (EGFR) inhibitor. Carbachol 11-14 caspase 3 Homo sapiens 157-166 22511543-5 2012 Carbachol (CCh), a muscarinic receptor-specific agonist, abrogated tumor necrosis factor alpha/interferon gamma-induced apoptosis through pathways involving caspase 3/7, but its cytoprotective effect was decreased by a M3R antagonist, a mitogen-activated protein kinase kinase/extracellular signal-regulated kinase (ERK) inhibitor, a phosphatidylinositol 3-kinase/Akt inhibitor, or an epidermal growth factor receptor (EGFR) inhibitor. Carbachol 11-14 mitogen-activated protein kinase 1 Homo sapiens 316-319 22511543-5 2012 Carbachol (CCh), a muscarinic receptor-specific agonist, abrogated tumor necrosis factor alpha/interferon gamma-induced apoptosis through pathways involving caspase 3/7, but its cytoprotective effect was decreased by a M3R antagonist, a mitogen-activated protein kinase kinase/extracellular signal-regulated kinase (ERK) inhibitor, a phosphatidylinositol 3-kinase/Akt inhibitor, or an epidermal growth factor receptor (EGFR) inhibitor. Carbachol 11-14 AKT serine/threonine kinase 1 Homo sapiens 364-367 22511543-5 2012 Carbachol (CCh), a muscarinic receptor-specific agonist, abrogated tumor necrosis factor alpha/interferon gamma-induced apoptosis through pathways involving caspase 3/7, but its cytoprotective effect was decreased by a M3R antagonist, a mitogen-activated protein kinase kinase/extracellular signal-regulated kinase (ERK) inhibitor, a phosphatidylinositol 3-kinase/Akt inhibitor, or an epidermal growth factor receptor (EGFR) inhibitor. Carbachol 11-14 epidermal growth factor receptor Homo sapiens 385-417 22511543-5 2012 Carbachol (CCh), a muscarinic receptor-specific agonist, abrogated tumor necrosis factor alpha/interferon gamma-induced apoptosis through pathways involving caspase 3/7, but its cytoprotective effect was decreased by a M3R antagonist, a mitogen-activated protein kinase kinase/extracellular signal-regulated kinase (ERK) inhibitor, a phosphatidylinositol 3-kinase/Akt inhibitor, or an epidermal growth factor receptor (EGFR) inhibitor. Carbachol 11-14 epidermal growth factor receptor Homo sapiens 419-423 22511543-6 2012 Ligation of M3R with CCh transactivated EGFR and phosphorylated ERK and Akt, the downstream targets of EGFR. Carbachol 21-24 epidermal growth factor receptor Homo sapiens 40-44 22511543-6 2012 Ligation of M3R with CCh transactivated EGFR and phosphorylated ERK and Akt, the downstream targets of EGFR. Carbachol 21-24 mitogen-activated protein kinase 1 Homo sapiens 64-67 22511543-6 2012 Ligation of M3R with CCh transactivated EGFR and phosphorylated ERK and Akt, the downstream targets of EGFR. Carbachol 21-24 AKT serine/threonine kinase 1 Homo sapiens 72-75 22511543-6 2012 Ligation of M3R with CCh transactivated EGFR and phosphorylated ERK and Akt, the downstream targets of EGFR. Carbachol 21-24 epidermal growth factor receptor Homo sapiens 103-107 22511543-8 2012 CCh stimulated Src phosphorylation and binding to EGFR. Carbachol 0-3 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 15-18 22511543-8 2012 CCh stimulated Src phosphorylation and binding to EGFR. Carbachol 0-3 epidermal growth factor receptor Homo sapiens 50-54 22717634-7 2012 After the intravenous infusion of carbachol, blood concentrations of Ang I and Ang II in near-term ovine fetus were both significantly increased (P < 0.05); however, blood concentration of vasopressin, values of blood gas, electrolytes and plasma osmolality in near-term ovine fetus were not significantly changed (P > 0.05). Carbachol 34-43 angiotensinogen Homo sapiens 69-74 22717634-7 2012 After the intravenous infusion of carbachol, blood concentrations of Ang I and Ang II in near-term ovine fetus were both significantly increased (P < 0.05); however, blood concentration of vasopressin, values of blood gas, electrolytes and plasma osmolality in near-term ovine fetus were not significantly changed (P > 0.05). Carbachol 34-43 angiotensinogen Homo sapiens 79-85 22717634-7 2012 After the intravenous infusion of carbachol, blood concentrations of Ang I and Ang II in near-term ovine fetus were both significantly increased (P < 0.05); however, blood concentration of vasopressin, values of blood gas, electrolytes and plasma osmolality in near-term ovine fetus were not significantly changed (P > 0.05). Carbachol 34-43 arginine vasopressin Homo sapiens 192-203 22717634-8 2012 Blood levels of Ang I and Ang II in the atropine (M receptor antagonist) + carbachol intravenous administration group was lower than those in the carbachol group without atropine administration (P < 0.05). Carbachol 75-84 angiotensinogen Homo sapiens 16-21 22717634-8 2012 Blood levels of Ang I and Ang II in the atropine (M receptor antagonist) + carbachol intravenous administration group was lower than those in the carbachol group without atropine administration (P < 0.05). Carbachol 75-84 angiotensinogen Homo sapiens 26-32 22717634-8 2012 Blood levels of Ang I and Ang II in the atropine (M receptor antagonist) + carbachol intravenous administration group was lower than those in the carbachol group without atropine administration (P < 0.05). Carbachol 146-155 angiotensinogen Homo sapiens 16-21 22717634-9 2012 In conclusion, this study indicates that the near-term changes of cardiovascular system induced by intravenous administration of carbachol in ovine fetus, such as blood pressure and heart rate, are associated with the changes of hormones of circulatory renin-angiotensin system. Carbachol 129-138 renin Homo sapiens 253-258 22449386-10 2012 SCA-9 cells exhibit a constitutive activation of NF-kappaB, which regulates carbachol-induced NOS2 and 3, arginase II and COX-1 expressions. Carbachol 76-85 mitochondrially encoded cytochrome c oxidase I Homo sapiens 122-127 22493444-5 2012 The inhibition of PI3K by PIK-93, LY294002, or wortmannin decreased carbachol-induced translocation of TRPC6 to the plasma membrane and carbachol-induced net Ca(2+) entry into T6.11 cells. Carbachol 68-77 transient receptor potential cation channel, subfamily C, member 6 Rattus norvegicus 103-108 22449386-7 2012 Phospholipase C (PLC)/nitric oxide synthase (NOS)/arginase pathway is involved in this effect, since carbachol stimulated nitric oxide production, increased NOS2 and NOS3 expressions, urea production, and arginase II expression (P<0.001). Carbachol 101-110 nitric oxide synthase 2 Homo sapiens 157-161 22449386-7 2012 Phospholipase C (PLC)/nitric oxide synthase (NOS)/arginase pathway is involved in this effect, since carbachol stimulated nitric oxide production, increased NOS2 and NOS3 expressions, urea production, and arginase II expression (P<0.001). Carbachol 101-110 nitric oxide synthase 3 Homo sapiens 166-170 22449386-8 2012 Also, phospholipase A2 (PLA2)/cyclooxygenase (COX) pathway is up-regulated in carbachol-induced SCA-9 cell proliferation, because prostaglandin E2 liberation (P<0.001) is increased and COX-1 expression is turned up (P<0.001). Carbachol 78-87 phospholipase A2 group IB Homo sapiens 6-22 22449386-8 2012 Also, phospholipase A2 (PLA2)/cyclooxygenase (COX) pathway is up-regulated in carbachol-induced SCA-9 cell proliferation, because prostaglandin E2 liberation (P<0.001) is increased and COX-1 expression is turned up (P<0.001). Carbachol 78-87 phospholipase A2 group IB Homo sapiens 24-28 22449386-8 2012 Also, phospholipase A2 (PLA2)/cyclooxygenase (COX) pathway is up-regulated in carbachol-induced SCA-9 cell proliferation, because prostaglandin E2 liberation (P<0.001) is increased and COX-1 expression is turned up (P<0.001). Carbachol 78-87 SCA9 Homo sapiens 96-101 22449386-8 2012 Also, phospholipase A2 (PLA2)/cyclooxygenase (COX) pathway is up-regulated in carbachol-induced SCA-9 cell proliferation, because prostaglandin E2 liberation (P<0.001) is increased and COX-1 expression is turned up (P<0.001). Carbachol 78-87 mitochondrially encoded cytochrome c oxidase I Homo sapiens 188-193 22449386-10 2012 SCA-9 cells exhibit a constitutive activation of NF-kappaB, which regulates carbachol-induced NOS2 and 3, arginase II and COX-1 expressions. Carbachol 76-85 SCA9 Homo sapiens 0-5 22449386-10 2012 SCA-9 cells exhibit a constitutive activation of NF-kappaB, which regulates carbachol-induced NOS2 and 3, arginase II and COX-1 expressions. Carbachol 76-85 nuclear factor kappa B subunit 1 Homo sapiens 49-58 22449386-10 2012 SCA-9 cells exhibit a constitutive activation of NF-kappaB, which regulates carbachol-induced NOS2 and 3, arginase II and COX-1 expressions. Carbachol 76-85 nitric oxide synthase 2 Homo sapiens 94-104 21951618-15 2012 In contrast, carbachol-induced TRPC6 channel activity was significantly inhibited (87.3%) by SCE in green fluorescent protein-TRPC6 pcDNA transfected HEK 293 cells. Carbachol 13-22 transient receptor potential cation channel subfamily C member 6 Homo sapiens 31-36 21951618-15 2012 In contrast, carbachol-induced TRPC6 channel activity was significantly inhibited (87.3%) by SCE in green fluorescent protein-TRPC6 pcDNA transfected HEK 293 cells. Carbachol 13-22 transient receptor potential cation channel subfamily C member 6 Homo sapiens 126-131 22173970-2 2012 Agonists, such as carbachol, and hormones that increase intracellular calcium concentration can activate AKT leading to cancer cell survival. Carbachol 18-27 AKT serine/threonine kinase 1 Homo sapiens 105-108 22173970-6 2012 Our goals were to examine the mechanism of carbachol activation of AKT and BAD in LNCaP prostate cancer cells and evaluate whether CaM KK may be mediating carbachol"s activation of AKT and cell survival. Carbachol 43-52 AKT serine/threonine kinase 1 Homo sapiens 67-70 22173970-6 2012 Our goals were to examine the mechanism of carbachol activation of AKT and BAD in LNCaP prostate cancer cells and evaluate whether CaM KK may be mediating carbachol"s activation of AKT and cell survival. Carbachol 155-164 calcium/calmodulin dependent protein kinase kinase 2 Homo sapiens 131-137 22173970-6 2012 Our goals were to examine the mechanism of carbachol activation of AKT and BAD in LNCaP prostate cancer cells and evaluate whether CaM KK may be mediating carbachol"s activation of AKT and cell survival. Carbachol 155-164 AKT serine/threonine kinase 1 Homo sapiens 181-184 22173970-7 2012 Our results suggest that carbachol treatment of LNCaP cells promoted cell survival through CaM KK and its phosphorylation of AKT. Carbachol 25-34 calcium/calmodulin dependent protein kinase kinase 2 Homo sapiens 91-97 22173970-7 2012 Our results suggest that carbachol treatment of LNCaP cells promoted cell survival through CaM KK and its phosphorylation of AKT. Carbachol 25-34 AKT serine/threonine kinase 1 Homo sapiens 125-128 22173970-8 2012 The bacterial toxin anisomycin triggered caspase-3 activation in LNCaP cells that was blocked by carbachol in a CaM KK- and AKT-dependent manner. Carbachol 97-106 caspase 3 Homo sapiens 41-50 22173970-8 2012 The bacterial toxin anisomycin triggered caspase-3 activation in LNCaP cells that was blocked by carbachol in a CaM KK- and AKT-dependent manner. Carbachol 97-106 calcium/calmodulin dependent protein kinase kinase 2 Homo sapiens 112-118 22173970-8 2012 The bacterial toxin anisomycin triggered caspase-3 activation in LNCaP cells that was blocked by carbachol in a CaM KK- and AKT-dependent manner. Carbachol 97-106 AKT serine/threonine kinase 1 Homo sapiens 124-127 22449386-11 2012 In addition, protein kinase C is involved in the up-regulation of NOS2 and arginase II enzymes induced by carbachol via NF-kappaB. Carbachol 106-115 nitric oxide synthase 2 Homo sapiens 66-70 22449386-11 2012 In addition, protein kinase C is involved in the up-regulation of NOS2 and arginase II enzymes induced by carbachol via NF-kappaB. Carbachol 106-115 nuclear factor kappa B subunit 1 Homo sapiens 120-129 22205227-3 2012 Muscle activation with KCl, carbachol (CCh), or prostaglandin E(2) at short muscle lengths can increase APS that is revealed by elevated pseudo-steady-state T(p) at longer lengths compared with prior T(p) measurements at those lengths, and APS generation is inhibited by the Rho Kinase (ROCK) inhibitor H-1152. Carbachol 28-37 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 275-285 22205227-3 2012 Muscle activation with KCl, carbachol (CCh), or prostaglandin E(2) at short muscle lengths can increase APS that is revealed by elevated pseudo-steady-state T(p) at longer lengths compared with prior T(p) measurements at those lengths, and APS generation is inhibited by the Rho Kinase (ROCK) inhibitor H-1152. Carbachol 28-37 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 287-291 22205227-3 2012 Muscle activation with KCl, carbachol (CCh), or prostaglandin E(2) at short muscle lengths can increase APS that is revealed by elevated pseudo-steady-state T(p) at longer lengths compared with prior T(p) measurements at those lengths, and APS generation is inhibited by the Rho Kinase (ROCK) inhibitor H-1152. Carbachol 39-42 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 275-285 22205227-3 2012 Muscle activation with KCl, carbachol (CCh), or prostaglandin E(2) at short muscle lengths can increase APS that is revealed by elevated pseudo-steady-state T(p) at longer lengths compared with prior T(p) measurements at those lengths, and APS generation is inhibited by the Rho Kinase (ROCK) inhibitor H-1152. Carbachol 39-42 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 287-291 22205227-6 2012 In addition, CCh-induced APS in whole mouse bladder was inhibited by H-1152, indicating that ROCK activity may regulate bladder compliance during filling. Carbachol 13-16 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 93-97 22351639-7 2012 Under conditions that specifically isolate apical Ca(2+)-activated Cl(-) channel (CaCC) currents, EGF pretreatment (100 ng ml(-1) for 15 min) potentiated carbachol (CCh)-induced responses to 173 +- 25% of those in control cells, when measured 24 h later (n = 26; P < 0.01). Carbachol 154-163 anoctamin 1 Homo sapiens 82-86 21957094-7 2012 Carbachol-induced myofibroblast transition was mediated by an increase in ERK1/2 phosphorylation, RhoA-GTP activation and cyclic monophosphate downregulation as well as by the autocrine TGF-beta1 release, which were effectively reduced by aclidinium. Carbachol 0-9 mitogen-activated protein kinase 3 Homo sapiens 74-80 22466505-2 2012 We found that carbachol-induced oscillations in rat CA3 have biphasic phase-response curves, consistent with the ability to couple with oscillations in afferent projections. Carbachol 14-23 carbonic anhydrase 3 Rattus norvegicus 52-55 21885397-10 2012 Carbachol stimulated release of LTB(4) from lung macrophages (buffer 222.3 +- 75.1 versus carbachol 1,118 +- 622.4 pg mL(-1); n = 15, p<0.05) but not IL-6 or IL-8. Carbachol 0-9 interleukin 6 Homo sapiens 155-159 21885397-10 2012 Carbachol stimulated release of LTB(4) from lung macrophages (buffer 222.3 +- 75.1 versus carbachol 1,118 +- 622.4 pg mL(-1); n = 15, p<0.05) but not IL-6 or IL-8. Carbachol 0-9 C-X-C motif chemokine ligand 8 Homo sapiens 163-167 22138972-7 2012 In scratch wound assays we also demonstrated (using the selective NHE inhibitor HOE-642) that carbachol and insulin like growth factor II (IGF-II) partly stimulate migration of HGMs in a NHE1-dependent manner. Carbachol 94-103 solute carrier family 9 member C1 Homo sapiens 66-69 22138972-7 2012 In scratch wound assays we also demonstrated (using the selective NHE inhibitor HOE-642) that carbachol and insulin like growth factor II (IGF-II) partly stimulate migration of HGMs in a NHE1-dependent manner. Carbachol 94-103 solute carrier family 9 member A1 Homo sapiens 187-191 22138972-11 2012 In addition, we demonstrated that NHE1 activity contributes to both IGF-II- and carbachol-stimulated migration and that it is obligatory for IGF-II-induced proliferation of HGMs. Carbachol 80-89 solute carrier family 9 member A1 Homo sapiens 34-38 22281988-6 2012 The overexpression of RGS5 impaired the release of Ca(2+) to thrombin, histamine, and carbachol, and reduced the contraction of precision-cut lung slices to carbachol. Carbachol 86-95 regulator of G protein signaling 5 Homo sapiens 22-26 22281988-6 2012 The overexpression of RGS5 impaired the release of Ca(2+) to thrombin, histamine, and carbachol, and reduced the contraction of precision-cut lung slices to carbachol. Carbachol 157-166 regulator of G protein signaling 5 Homo sapiens 22-26 21692984-11 2012 Addition of the PKC activator, phorbol 12-myristate 13-acetate and the specific PKC(epsilon) agonist, carbachol, blocked the effects of nicorandil on connexin43 phosphorylation and dye permeability. Carbachol 102-111 gap junction protein, alpha 1 Rattus norvegicus 150-160 21899666-8 2012 CCh was found to reorganize alpha-fodrin in HSG cells in a Ca(2+) -dependent manner. Carbachol 0-3 spectrin alpha, non-erythrocytic 1 Homo sapiens 28-40 21899666-9 2012 However, pretreatment with SS IgG prevented the cytoskeletal reorganization of alpha-fodrin induced by CCh. Carbachol 103-106 spectrin alpha, non-erythrocytic 1 Homo sapiens 79-91 22197203-8 2012 TRK-380 had a concentration-dependent relaxing effect on the contractile responses to carbachol and KCl in human detrusor strips. Carbachol 86-95 neurotrophic receptor tyrosine kinase 1 Homo sapiens 0-3 21254300-12 2012 Moreover, we could also show that both chemical late-LTP and carbachol-reinforced early-LTP-induced STC processes are mediated by the neurotrophin BDNF. Carbachol 61-70 brain derived neurotrophic factor Homo sapiens 147-151 21957094-7 2012 Carbachol-induced myofibroblast transition was mediated by an increase in ERK1/2 phosphorylation, RhoA-GTP activation and cyclic monophosphate downregulation as well as by the autocrine TGF-beta1 release, which were effectively reduced by aclidinium. Carbachol 0-9 ras homolog family member A Homo sapiens 98-102 21957094-7 2012 Carbachol-induced myofibroblast transition was mediated by an increase in ERK1/2 phosphorylation, RhoA-GTP activation and cyclic monophosphate downregulation as well as by the autocrine TGF-beta1 release, which were effectively reduced by aclidinium. Carbachol 0-9 transforming growth factor beta 1 Homo sapiens 186-195 22069319-13 2012 IFN-gamma also abrogates the ability of EGF to inhibit carbachol-stimulated basolateral K(+) currents. Carbachol 55-64 interferon gamma Mus musculus 0-9 21956166-12 2012 Biotinylation studies revealed that carbachol inhibits oxalate transport by reducing SLC26A6 surface expression. Carbachol 36-45 solute carrier family 26 member 6 Homo sapiens 85-92 21956166-7 2012 Cholinergic stimulation with carbachol modulates intestinal ion transport through signaling pathways including PKC activation. Carbachol 29-38 protein kinase C delta Homo sapiens 111-114 21956166-13 2012 We conclude that carbachol negatively regulates oxalate transport by reducing SLC26A6 surface expression in T84 cells through signaling pathways including the M(3) muscarinic receptor, phospholipase C, PKC-delta, and c-Src. Carbachol 17-26 solute carrier family 26 member 6 Homo sapiens 78-85 21956166-10 2012 Carbachol also led to significant translocation of PKC-delta from the cytosol to the membrane of T84 cells. Carbachol 0-9 protein kinase C delta Homo sapiens 51-60 21956166-13 2012 We conclude that carbachol negatively regulates oxalate transport by reducing SLC26A6 surface expression in T84 cells through signaling pathways including the M(3) muscarinic receptor, phospholipase C, PKC-delta, and c-Src. Carbachol 17-26 cholinergic receptor muscarinic 3 Homo sapiens 159-183 21956166-11 2012 Using pharmacological inhibitors, we observed that carbachol inhibits oxalate transport through the M(3) muscarinic receptor and phospholipase C. Utilizing the Src inhibitor PP2 and phosphorylation studies, we found that the observed regulation downstream of PKC-delta is partially mediated by c-Src. Carbachol 51-60 cholinergic receptor muscarinic 3 Homo sapiens 100-124 21956166-11 2012 Using pharmacological inhibitors, we observed that carbachol inhibits oxalate transport through the M(3) muscarinic receptor and phospholipase C. Utilizing the Src inhibitor PP2 and phosphorylation studies, we found that the observed regulation downstream of PKC-delta is partially mediated by c-Src. Carbachol 51-60 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 160-163 21956166-13 2012 We conclude that carbachol negatively regulates oxalate transport by reducing SLC26A6 surface expression in T84 cells through signaling pathways including the M(3) muscarinic receptor, phospholipase C, PKC-delta, and c-Src. Carbachol 17-26 protein kinase C delta Homo sapiens 202-211 21956166-13 2012 We conclude that carbachol negatively regulates oxalate transport by reducing SLC26A6 surface expression in T84 cells through signaling pathways including the M(3) muscarinic receptor, phospholipase C, PKC-delta, and c-Src. Carbachol 17-26 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 217-222 21956166-11 2012 Using pharmacological inhibitors, we observed that carbachol inhibits oxalate transport through the M(3) muscarinic receptor and phospholipase C. Utilizing the Src inhibitor PP2 and phosphorylation studies, we found that the observed regulation downstream of PKC-delta is partially mediated by c-Src. Carbachol 51-60 neuropeptide Y receptor Y6 (pseudogene) Homo sapiens 174-177 22613970-5 2012 We found that the muscarinic agonist carbachol and the ryanodine receptor agonists caffeine and 4-chloro-m-cresol had more potent depolarizing effects on Anx7(+/-) beta-cells compared to controls. Carbachol 37-46 annexin A7 Mus musculus 154-158 21956166-11 2012 Using pharmacological inhibitors, we observed that carbachol inhibits oxalate transport through the M(3) muscarinic receptor and phospholipase C. Utilizing the Src inhibitor PP2 and phosphorylation studies, we found that the observed regulation downstream of PKC-delta is partially mediated by c-Src. Carbachol 51-60 protein kinase C delta Homo sapiens 259-268 21956166-11 2012 Using pharmacological inhibitors, we observed that carbachol inhibits oxalate transport through the M(3) muscarinic receptor and phospholipase C. Utilizing the Src inhibitor PP2 and phosphorylation studies, we found that the observed regulation downstream of PKC-delta is partially mediated by c-Src. Carbachol 51-60 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 294-299 22916223-6 2012 Stimulation of human tracheal mucosa with PAR2-activating peptide (PAR2-AP) elevated intracellular Ca(2+) and induced glandular secretion equal to approximately 30% of the carbachol response in the human airway. Carbachol 172-181 F2R like trypsin receptor 1 Homo sapiens 42-46 21883831-8 2012 Rho-kinase inhibitor Y27632 (10 microM) inhibited peak and sustained contractile responses to carbachol in control bladders (peak by 38%; plateau 57%) and obstructed bladders (peak 37% plateau 47%). Carbachol 94-103 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 0-10 22972200-8 2012 PrP-positive cells with automaticity showed positive and negative chronotropic responses to isoproterenol and carbamylcholine, respectively. Carbachol 110-125 prion protein Mus musculus 0-3 22916223-6 2012 Stimulation of human tracheal mucosa with PAR2-activating peptide (PAR2-AP) elevated intracellular Ca(2+) and induced glandular secretion equal to approximately 30% of the carbachol response in the human airway. Carbachol 172-181 coagulation factor II (thrombin) receptor-like 1 Mus musculus 67-71 21945499-9 2011 Furthermore, by introduction of miR-145, ES-pre-SMC proliferation was significantly inhibited and carbachol-stimulated contraction of ES-pre-SMCs was significantly increased. Carbachol 98-107 microRNA 145 Homo sapiens 32-39 23115638-7 2012 We report that Orai1-mediated Ca(2+) entry generates [Ca(2+)](i) oscillations at different [CCh], ranging from very low to high. Carbachol 92-95 ORAI calcium release-activated calcium modulator 1 Homo sapiens 15-20 23115638-8 2012 In contrast, TRPC1-mediated Ca(2+) entry generates sustained [Ca(2+)](i) elevation at high [CCh] and contributes to frequency of [Ca(2+)](i) oscillations at lower [agonist]. Carbachol 92-95 transient receptor potential cation channel subfamily C member 1 Homo sapiens 13-18 21884684-7 2011 In hippocampal slices carbachol increased the levels of two extracellular matrix protein, fibronectin and laminin-1, by 1.6-fold, as measured by Western blot. Carbachol 22-31 fibronectin 1 Rattus norvegicus 90-101 21884684-7 2011 In hippocampal slices carbachol increased the levels of two extracellular matrix protein, fibronectin and laminin-1, by 1.6-fold, as measured by Western blot. Carbachol 22-31 laminin subunit alpha 1 Rattus norvegicus 106-115 21884684-10 2011 Furthermore, function-blocking fibronectin or laminin-1 antibodies antagonized the effect of carbachol on neurite outgrowth. Carbachol 93-102 fibronectin 1 Rattus norvegicus 31-42 21884684-10 2011 Furthermore, function-blocking fibronectin or laminin-1 antibodies antagonized the effect of carbachol on neurite outgrowth. Carbachol 93-102 laminin subunit alpha 1 Rattus norvegicus 46-55 21884684-12 2011 By decreasing fibronectin and laminin levels ethanol prevents carbachol-induced neuritogenesis. Carbachol 62-71 fibronectin 1 Rattus norvegicus 14-25 21933938-4 2011 Interactions among carbachol, PKC inhibitors, phorbol 12-myristate 13-acetate, and thapsigargin to modulate [Ca(2+)](i) implicated conventional PKC isoforms in mediating sustained secretion. Carbachol 19-28 proline rich transmembrane protein 2 Homo sapiens 144-147 21933938-5 2011 With increasing times during carbachol perfusion of glands, in situ, PKC-alpha redistributed across glandular membrane compartments and underwent a rapid and persistent accumulation near the luminal borders of mucous cells. Carbachol 29-38 protein kinase C alpha Homo sapiens 69-78 22004286-5 2011 The PKC inhibitor Go6976 (1mumol L(-1) ) inhibited the carbachol-induced phosphorylation of CPI-17, whereas the Rho-associated kinase (ROCK) inhibitor, Y-27632 (10mumol L(-1) ) inhibited the carbachol-induced phosphorylation of both CPI-17 and MYPT1. Carbachol 55-64 protein phosphatase 1, regulatory (inhibitor) subunit 14A Rattus norvegicus 92-98 22004286-5 2011 The PKC inhibitor Go6976 (1mumol L(-1) ) inhibited the carbachol-induced phosphorylation of CPI-17, whereas the Rho-associated kinase (ROCK) inhibitor, Y-27632 (10mumol L(-1) ) inhibited the carbachol-induced phosphorylation of both CPI-17 and MYPT1. Carbachol 55-64 protein phosphatase 1, regulatory (inhibitor) subunit 14A Rattus norvegicus 233-239 22004286-5 2011 The PKC inhibitor Go6976 (1mumol L(-1) ) inhibited the carbachol-induced phosphorylation of CPI-17, whereas the Rho-associated kinase (ROCK) inhibitor, Y-27632 (10mumol L(-1) ) inhibited the carbachol-induced phosphorylation of both CPI-17 and MYPT1. Carbachol 55-64 protein phosphatase 1, regulatory subunit 12A Rattus norvegicus 244-249 22004286-5 2011 The PKC inhibitor Go6976 (1mumol L(-1) ) inhibited the carbachol-induced phosphorylation of CPI-17, whereas the Rho-associated kinase (ROCK) inhibitor, Y-27632 (10mumol L(-1) ) inhibited the carbachol-induced phosphorylation of both CPI-17 and MYPT1. Carbachol 191-200 protein phosphatase 1, regulatory (inhibitor) subunit 14A Rattus norvegicus 92-98 22001099-5 2011 Carbachol (1 muM) increased the spontaneous contractile rate of these tissue strips by 122% +- 27% (P < .001). Carbachol 0-9 latexin Homo sapiens 13-16 22001099-8 2011 Darifenacin, oxybutynin, tolterodine, and solifenacin (1 muM) all significantly depressed the frequency responses to carbachol (1 muM). Carbachol 117-126 latexin Homo sapiens 57-60 22001099-8 2011 Darifenacin, oxybutynin, tolterodine, and solifenacin (1 muM) all significantly depressed the frequency responses to carbachol (1 muM). Carbachol 117-126 latexin Homo sapiens 130-133 21859823-3 2011 We first verified that cannabinoids (CP55,940 and WIN55,212-2) readily suppressed carbachol-induced gamma oscillations in the CA3 region of hippocampal slices via activation of CB(1)Rs. Carbachol 82-91 carbonic anhydrase 3 Homo sapiens 126-129 22937150-5 2012 Myocardin overexpression from day 10 to day 28 of embryoid body differentiation increased the number of smooth muscle alpha-actin(+) and smooth muscle myosin heavy chain(+) SMC-like cells and increased carbachol-induced contractile function. Carbachol 202-211 myocardin Homo sapiens 0-9 22004286-4 2011 Key Results In rat ileal smooth muscle, phosphorylation level of CPI-17 at Thr(38) and MYPT1 at Thr(853) , but not MYPT1 at Thr(696) , were increased with carbachol (1mumolL(-1) ) accompanied with muscle contraction. Carbachol 156-165 protein phosphatase 1, regulatory (inhibitor) subunit 14A Rattus norvegicus 66-72 22004286-4 2011 Key Results In rat ileal smooth muscle, phosphorylation level of CPI-17 at Thr(38) and MYPT1 at Thr(853) , but not MYPT1 at Thr(696) , were increased with carbachol (1mumolL(-1) ) accompanied with muscle contraction. Carbachol 156-165 protein phosphatase 1, regulatory subunit 12A Rattus norvegicus 88-93 21940627-5 2011 Carbachol, a known nAChR and muscarinic receptor agonist, up-regulated both alpha4beta2 nAChR binding sites and subunit protein 2-fold more than did nicotine. Carbachol 0-9 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 19-24 21940627-5 2011 Carbachol, a known nAChR and muscarinic receptor agonist, up-regulated both alpha4beta2 nAChR binding sites and subunit protein 2-fold more than did nicotine. Carbachol 0-9 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 88-93 21874246-7 2011 In addition, ghrelin enhanced smooth muscle strip contraction induced by carbachol. Carbachol 73-82 ghrelin and obestatin prepropeptide Rattus norvegicus 13-20 21567383-5 2011 Carbachol was synthesized with an active choline group and was conjugated with an siRNA that targets caspase 3. Carbachol 0-9 caspase 3 Homo sapiens 101-110 21684833-11 2011 After ozone exposure, bronchial rings derived from mindin-/- mice demonstrated reduced constriction in response to carbachol. Carbachol 115-124 spondin 2, extracellular matrix protein Mus musculus 51-57 21410689-3 2011 EXPERIMENTAL APPROACH Effects of BNP on responses to carbachol and histamine were evaluated in non-sensitized, passively sensitized, epithelium-intact or denuded isolated bronchi and in the presence of methoctramine, N(omega) -nitro-L-arginine methyl ester (L-NAME) and aminoguanidine. Carbachol 53-62 natriuretic peptide B Homo sapiens 33-36 21918251-6 2011 RESULTS: According to conducted testing, activation of soluble guanylyl cyclase with the use of YC-1 and 8Br cGMP caused reduced reaction of the tracheal smooth muscle with carbachol on average to 80%. Carbachol 173-182 glutathione S-transferase alpha 1 Rattus norvegicus 96-106 21306750-7 2011 Insulin secretion induced by the protein kinase A (PKA) activators forskolin and 3-isobutyl-1-methyl-xanthine, in the presence of 11.1 mmol/L glucose, was lower in LDLR(-/-) islets and was normalized in the presence of the protein kinase C pathway activators carbachol and phorbol 12-myristate 13-acetate. Carbachol 259-268 low density lipoprotein receptor Mus musculus 164-168 20805763-8 2011 RESULTS: Carbachol inhibited expression of TNF-alpha and IL-6 after LPS injection and had no significant effect on IL-10 in rat endotoxemia model. Carbachol 9-18 tumor necrosis factor Rattus norvegicus 43-52 21371005-6 2011 KEY RESULTS: Ang-(1-7)-treated apoE (-/-) mice showed improved renal endothelium-dependent vasorelaxation induced by carbachol and increased renal basal cGMP production, compared with untreated apoE (-/-) mice. Carbachol 117-126 apolipoprotein E Mus musculus 31-35 21402151-7 2011 Phosphorylation of TRPC7 significantly suppressed carbachol-induced calcium influx and CREB phosphorylation. Carbachol 50-59 transient receptor potential cation channel, subfamily C, member 7 Mus musculus 19-24 21402151-7 2011 Phosphorylation of TRPC7 significantly suppressed carbachol-induced calcium influx and CREB phosphorylation. Carbachol 50-59 cAMP responsive element binding protein 1 Mus musculus 87-91 20805763-8 2011 RESULTS: Carbachol inhibited expression of TNF-alpha and IL-6 after LPS injection and had no significant effect on IL-10 in rat endotoxemia model. Carbachol 9-18 interleukin 6 Rattus norvegicus 57-61 21570469-4 2011 Carbachol- and PDBu-induced sAPPalpha secretions were blocked by the general PKC inhibitor GF109203X. Carbachol 0-9 protein kinase C alpha Homo sapiens 77-80 20805763-10 2011 Cell experiments also showed that increases of TNF-alpha and IL-6 after LPS stimulation could be significantly inhibited by carbachol or nicotine, whereas IL-10 was not apparently altered. Carbachol 124-133 tumor necrosis factor Rattus norvegicus 47-56 20805763-10 2011 Cell experiments also showed that increases of TNF-alpha and IL-6 after LPS stimulation could be significantly inhibited by carbachol or nicotine, whereas IL-10 was not apparently altered. Carbachol 124-133 interleukin 6 Rattus norvegicus 61-65 21338617-1 2011 In SH-SY5Y human neuroblastoma cells, the cholinergic agonist, carbachol, stimulates phosphorylation of the small heat shock protein 27 (HSP27). Carbachol 63-72 heat shock protein family B (small) member 1 Homo sapiens 114-135 21338617-1 2011 In SH-SY5Y human neuroblastoma cells, the cholinergic agonist, carbachol, stimulates phosphorylation of the small heat shock protein 27 (HSP27). Carbachol 63-72 heat shock protein family B (small) member 1 Homo sapiens 137-142 21338617-4 2011 This response to carbachol is partially reduced by inhibition of protein kinase C (PKC) with GF 109203X and p38 mitogen-activated protein kinase (MAPK) with SB 203580. Carbachol 17-26 mitogen-activated protein kinase 14 Homo sapiens 108-144 21338617-8 2011 SH-SY5Y cells differentiated with a low concentration of PDB and basic fibroblast growth factor to a more neuronal phenotype retain carbachol-, PDB- and Akti-1/2-responsive HSP27 phosphorylation. Carbachol 132-141 heat shock protein family B (small) member 1 Homo sapiens 173-178 21338617-9 2011 Immunofluorescence microscopy confirms increased HSP27 phosphorylation in response to carbachol or PDB. Carbachol 86-95 heat shock protein family B (small) member 1 Homo sapiens 49-54 21497590-5 2011 Activation of BKCa channels by NS1619 had a minor inhibitory effect on carbachol-induced phasic activity of bladder strips from control and diabetic rats, and significantly inhibited amplitude only at 30 muM. Carbachol 71-80 potassium calcium-activated channel subfamily M alpha 1 Rattus norvegicus 14-18 20705939-3 2011 We found that the carbachol-stimulated cytoskeletal recruitment of actin-related protein-3 (Arp3), metavinculin, and talin were up-regulated at short muscle lengths and down-regulated at long muscle lengths, suggesting that the actin cytoskeleton--integrin complex becomes enriched in cross-linked and branched actin filaments in shortened ASM. Carbachol 18-27 actin related protein 3 Homo sapiens 67-90 20705939-3 2011 We found that the carbachol-stimulated cytoskeletal recruitment of actin-related protein-3 (Arp3), metavinculin, and talin were up-regulated at short muscle lengths and down-regulated at long muscle lengths, suggesting that the actin cytoskeleton--integrin complex becomes enriched in cross-linked and branched actin filaments in shortened ASM. Carbachol 18-27 actin related protein 3 Homo sapiens 92-96 20705939-3 2011 We found that the carbachol-stimulated cytoskeletal recruitment of actin-related protein-3 (Arp3), metavinculin, and talin were up-regulated at short muscle lengths and down-regulated at long muscle lengths, suggesting that the actin cytoskeleton--integrin complex becomes enriched in cross-linked and branched actin filaments in shortened ASM. Carbachol 18-27 vinculin Homo sapiens 99-111 21527320-5 2011 When recording carbachol-evoked inhibitory postsynaptic currents (IPSCs) which presumably originate from CB1 expressing cholecystokinin (CCK) interneurons, we found that depolarization-induced suppression of inhibition (DSI) was impaired by the stress. Carbachol 15-24 cannabinoid receptor 1 Rattus norvegicus 105-108 21527320-5 2011 When recording carbachol-evoked inhibitory postsynaptic currents (IPSCs) which presumably originate from CB1 expressing cholecystokinin (CCK) interneurons, we found that depolarization-induced suppression of inhibition (DSI) was impaired by the stress. Carbachol 15-24 cholecystokinin Rattus norvegicus 137-140 21474425-9 2011 Desensitization of intracolonic TRPV1 receptors before the induction of acute colitis restored the response of isolated detrusor strips to CCh but not to EFS stimulation. Carbachol 139-142 transient receptor potential cation channel, subfamily V, member 1 Rattus norvegicus 32-37 21265824-5 2011 The Rho-kinase inhibitors decreased the spontaneous contractions and the responses to carbachol and substance P independently of neuronal inputs, suggesting Y-27632 acts directly on smooth muscle. Carbachol 86-95 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 4-14 21265824-6 2011 The Rho-kinase inhibitors significantly reduced the depolarization in response to carbachol, an effect that cannot be due to regulation of Ca(2+) sensitization. Carbachol 82-91 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 4-14 21265824-8 2011 CONCLUSIONS AND IMPLICATIONS: The Rho-kinase inhibitors decreased contractions evoked by nerve stimulation, carbachol and substance P. Carbachol 108-117 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 34-44 21466795-1 2011 Microinjection of the cholinergic agonist carbachol into the bed nucleus of the stria terminalis (BST) has been reported to cause pressor response in unanesthetized rats, which was shown to be mediated by an acute release of vasopressin into the systemic circulation and followed by baroreflex-mediated bradycardia. Carbachol 42-51 arginine vasopressin Rattus norvegicus 225-236 21466795-5 2011 Moreover, BST treatment with carbachol significantly increased plasma vasopressin levels, thus confirming previous evidences that carbachol microinjection into the BST evokes pressor response due to vasopressin release into the circulation. Carbachol 29-38 arginine vasopressin Rattus norvegicus 70-81 21466795-5 2011 Moreover, BST treatment with carbachol significantly increased plasma vasopressin levels, thus confirming previous evidences that carbachol microinjection into the BST evokes pressor response due to vasopressin release into the circulation. Carbachol 29-38 arginine vasopressin Rattus norvegicus 199-210 21466795-5 2011 Moreover, BST treatment with carbachol significantly increased plasma vasopressin levels, thus confirming previous evidences that carbachol microinjection into the BST evokes pressor response due to vasopressin release into the circulation. Carbachol 130-139 arginine vasopressin Rattus norvegicus 70-81 21466795-5 2011 Moreover, BST treatment with carbachol significantly increased plasma vasopressin levels, thus confirming previous evidences that carbachol microinjection into the BST evokes pressor response due to vasopressin release into the circulation. Carbachol 130-139 arginine vasopressin Rattus norvegicus 199-210 21453700-5 2011 Results show that multiple pathways are involved in the effects of carbachol on astrocyte-mediated increases in fibronectin expression and neuritogenesis. Carbachol 67-76 fibronectin 1 Rattus norvegicus 112-123 21406187-4 2011 HD (2 or 15 days) reduced the pressor responses to ANG II (50 ng/1mul) icv (8+-3 and 11+-3 mm Hg, respectively, vs. sham: 23+-3 and 21+-2 mm Hg) or carbachol (4 nmol/1 mul) icv (8+-2 and 21+-3 mm Hg, respectively, vs. sham: 33+-3 and 33+-3 mm Hg), without changing baseline arterial pressure. Carbachol 148-157 angiotensinogen Rattus norvegicus 51-57 21311027-8 2011 Furthermore, carbachol-induced expression of phospho-AKT (a marker of cholinergic response) was lost from day 35 CSMC in vitro, while retained in control cells. Carbachol 13-22 AKT serine/threonine kinase 1 Rattus norvegicus 53-56 21212180-9 2011 Incubation with catalytically inactive PLD1, but not catalytically inactive mutant PLD2 adenovirus, also increased Cch-stimulated protein secretion and decreased ERK activity. Carbachol 115-118 phospholipase D1 Rattus norvegicus 39-43 21713709-5 2011 RESULTS: In vitro, ghrelin enhanced the contraction of smooth muscle strips in the presence of carbachol, and the differences in contraction induced by different concentrations of ghrelin(0.1, 0.5, 1.0 mumol/L) were statistically significant [(223+-18)%, (245+-22)%, (264+-25)%, P<0.01]. Carbachol 95-104 ghrelin and obestatin prepropeptide Rattus norvegicus 19-26 20958290-9 2011 Carbachol affected both GDP association with and dissociation from G(i/o) G-proteins but only its dissociation from G(s/olf) G-proteins. Carbachol 0-9 transmembrane O-mannosyltransferase targeting cadherins 1 Homo sapiens 120-123 21384922-10 2011 Phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) was increased in both carbachol-stimulated and epiphora glands. Carbachol 92-101 mitogen-activated protein kinase 1 Homo sapiens 19-60 21384922-10 2011 Phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) was increased in both carbachol-stimulated and epiphora glands. Carbachol 92-101 mitogen-activated protein kinase 3 Homo sapiens 62-68 21384922-11 2011 Preincubation of submandibular glands with ERK1/2 inhibitors PD98059 or U0126 inhibited carbachol-induced F-actin redistribution. Carbachol 88-97 mitogen-activated protein kinase 3 Homo sapiens 43-49 21463572-7 2011 Carbachol stimulation releases Gbetagamma subunits from caveolae with a concurrent stabilization of activated Galpha(q) by caveolin-3 (Cav3). Carbachol 0-9 caveolin 3 Canis lupus familiaris 123-133 21463572-7 2011 Carbachol stimulation releases Gbetagamma subunits from caveolae with a concurrent stabilization of activated Galpha(q) by caveolin-3 (Cav3). Carbachol 0-9 caveolin 3 Canis lupus familiaris 135-139 21239638-12 2011 In conclusions, while Cx43(G60S/+) mice had severe AT/F, Cx40(-/-) mice were resistant to CCh-induced AT/F. Carbachol 90-93 gap junction protein, alpha 5 Mus musculus 57-61 20082292-5 2011 Here we show that, in layer V of rat medial entorhinal cortex, muscarinic receptor-evoked plateau potentials and persistent firing induced by carbachol require phospholipase C activation, decrease of PIP(2) levels, and permissive intracellular Ca(2+) concentrations. Carbachol 142-151 prolactin induced protein Rattus norvegicus 200-203 21278382-7 2011 PCLS from Rgs5(-/-) mice contracted more to carbachol than those from WT mice, indicating that RGS5 negatively regulates bronchial smooth muscle contraction. Carbachol 44-53 regulator of G-protein signaling 5 Mus musculus 10-14 21278382-7 2011 PCLS from Rgs5(-/-) mice contracted more to carbachol than those from WT mice, indicating that RGS5 negatively regulates bronchial smooth muscle contraction. Carbachol 44-53 regulator of G-protein signaling 5 Mus musculus 95-99 21212180-10 2011 Inhibition of Rho with C3 exotoxin and a dominant negative Rho adenovirus and inhibition of ROCK with Y-27632 inhibited Cch-stimulated PLD1 activity, increased protein secretion, and decreased ERK activity. Carbachol 120-123 phospholipase D1 Rattus norvegicus 135-139 21212180-10 2011 Inhibition of Rho with C3 exotoxin and a dominant negative Rho adenovirus and inhibition of ROCK with Y-27632 inhibited Cch-stimulated PLD1 activity, increased protein secretion, and decreased ERK activity. Carbachol 120-123 Eph receptor B1 Rattus norvegicus 193-196 21212180-11 2011 The association of PLD1 and ROCK increased with Cch stimulation, as determined by immunoprecipitation. Carbachol 48-51 phospholipase D1 Rattus norvegicus 19-23 20525722-4 2011 Carbachol increased MUC5AC mRNA and protein expression in human bronchus and cultured epithelial cells. Carbachol 0-9 mucin 5AC, oligomeric mucus/gel-forming Homo sapiens 20-26 21081155-10 2011 Exposure to carbamylcholine decreased GDNF protein content to 51%+-28% of controls in the EDL but not SOL. Carbachol 12-27 glial cell derived neurotrophic factor Rattus norvegicus 38-42 20525722-5 2011 Aclidinium inhibited the carbachol-induced MUC5AC mRNA and protein expression with potency (half maximal inhibitory concentration) ~1 nM in human bronchus and cultured airway epithelial cells. Carbachol 25-34 mucin 5AC, oligomeric mucus/gel-forming Homo sapiens 43-49 20525722-6 2011 AG1478, a selective inhibitor of epidermal growth factor receptor (EGFR) tyrosine kinase, inhibited carbachol-induced MUC5AC responses, indicating EGFR transactivation. Carbachol 100-109 epidermal growth factor receptor Homo sapiens 33-65 20525722-6 2011 AG1478, a selective inhibitor of epidermal growth factor receptor (EGFR) tyrosine kinase, inhibited carbachol-induced MUC5AC responses, indicating EGFR transactivation. Carbachol 100-109 epidermal growth factor receptor Homo sapiens 67-71 20525722-6 2011 AG1478, a selective inhibitor of epidermal growth factor receptor (EGFR) tyrosine kinase, inhibited carbachol-induced MUC5AC responses, indicating EGFR transactivation. Carbachol 100-109 mucin 5AC, oligomeric mucus/gel-forming Homo sapiens 118-124 20525722-6 2011 AG1478, a selective inhibitor of epidermal growth factor receptor (EGFR) tyrosine kinase, inhibited carbachol-induced MUC5AC responses, indicating EGFR transactivation. Carbachol 100-109 epidermal growth factor receptor Homo sapiens 147-151 20525722-7 2011 Aclidinium inhibited carbachol-induced phospho-EGFR and phospho-p44/42 MAPK expression. Carbachol 21-30 epidermal growth factor receptor Homo sapiens 47-51 20525722-8 2011 In cultured airway epithelial cells transfected with small interfering (si)RNA against muscarinic receptor subtypes, siRNA-M3 but not siRNA-M2 blocked carbachol-induced MUC5AC expression. Carbachol 151-160 mucin 5AC, oligomeric mucus/gel-forming Homo sapiens 169-175 21268094-6 2011 All the conditions known to modulate mTOR activity (IGF-1, wortmannin, rapamycin, PP242, and nutrient starvation) were shown to modify carbachol-induced Ca(2+) signaling in RINm5F cells. Carbachol 135-144 mechanistic target of rapamycin kinase Rattus norvegicus 37-41 21268094-6 2011 All the conditions known to modulate mTOR activity (IGF-1, wortmannin, rapamycin, PP242, and nutrient starvation) were shown to modify carbachol-induced Ca(2+) signaling in RINm5F cells. Carbachol 135-144 insulin-like growth factor 1 Rattus norvegicus 52-57 21160001-8 2011 At the Schaffer-collateral synapse, bath application of the cholinergic agonist carbachol suppressed stratum radiatum-evoked excitatory postsynaptic potentials (EPSPs) in wild-type CA1 neurons and in CA1 neurons from mice lacking M1 or M2 receptors. Carbachol 80-89 carbonic anhydrase 1 Mus musculus 200-203 21056967-8 2011 Treatment of H508 cells with carbachol, a hydrolytically stable acetylcholine analog, promoted H508 cell proliferation and activation of the mitogenic kinase, p90RSK. Carbachol 29-38 ribosomal protein S6 kinase A1 Homo sapiens 159-165 21056967-9 2011 Small interfering RNA-mediated knockdown of Tmem147 expression significantly augmented the stimulatory effects of carbachol on H508 cell proliferation and p90RSK activation. Carbachol 114-123 transmembrane protein 147 Homo sapiens 44-51 20939850-8 2011 Conditioned medium from CCh-pretreated cells mimicked its chronic antisecretory actions, suggesting involvement of an epithelial-derived soluble factor but further experimentation ruled out the involvement of epidermal growth factor receptor ligands. Carbachol 24-27 epidermal growth factor receptor Homo sapiens 209-241 21298058-2 2011 METHODOLOGY/PRINCIPAL FINDINGS: Here we examined carbachol-induced gamma oscillations in hippocampal slices lacking BDNF gene in the area CA3. Carbachol 49-58 carbonic anhydrase 3 Mus musculus 138-141 21044662-4 2011 In the present study we examined the c-fos expression of these neurons after carbachol-induced active sleep (C-AS). Carbachol 77-86 Fos proto-oncogene, AP-1 transcription factor subunit Felis catus 37-42 21030607-7 2011 The cholinergic agonist carbachol rapidly (within 10 min) reduced cell volume along the entire crypt/villus axis and promoted NHE3 internalization into early endosomes. Carbachol 24-33 solute carrier family 9 member A3 Homo sapiens 126-130 21030607-8 2011 In contrast, carbachol induced membrane recruitment of NKCC1 and CFTR in all crypt and villus enterocytes, NKCC1 in all goblet cells, and NBCe1 in all villus enterocytes. Carbachol 13-22 solute carrier family 12 member 2 Homo sapiens 55-60 21030607-8 2011 In contrast, carbachol induced membrane recruitment of NKCC1 and CFTR in all crypt and villus enterocytes, NKCC1 in all goblet cells, and NBCe1 in all villus enterocytes. Carbachol 13-22 CF transmembrane conductance regulator Homo sapiens 65-69 21030607-8 2011 In contrast, carbachol induced membrane recruitment of NKCC1 and CFTR in all crypt and villus enterocytes, NKCC1 in all goblet cells, and NBCe1 in all villus enterocytes. Carbachol 13-22 solute carrier family 12 member 2 Homo sapiens 107-112 22178951-5 2011 Also, besides this last mutant was able to physically couple to Galpha(q/11) after carbachol challenge it was neither capable to activate phospholipase C nor phospholipase D. On the other hand, we demonstrated that the Asn-7.49 is important for the interaction between M(3)R and ARF1 and also for the formation of the ARF/Rho/beta gamma signaling complex, a complex that might determine the rapid activation and desensitization of PLD. Carbachol 83-92 succinate-CoA ligase GDP/ADP-forming subunit alpha Homo sapiens 64-75 21160001-8 2011 At the Schaffer-collateral synapse, bath application of the cholinergic agonist carbachol suppressed stratum radiatum-evoked excitatory postsynaptic potentials (EPSPs) in wild-type CA1 neurons and in CA1 neurons from mice lacking M1 or M2 receptors. Carbachol 80-89 carbonic anhydrase 1 Mus musculus 181-184 20851763-4 2011 Using serum starved SK-N-SH neuroblastoma cells, we show that the muscarinic receptor agonists carbachol and pilocarpine enhance the activation of the mTOR substrate p70 S6 Kinase (S6K) and its target ribosomal protein S6 (S6) in a VEGFR2 dependent manner. Carbachol 95-104 mechanistic target of rapamycin kinase Homo sapiens 151-155 20727967-4 2011 Pretreatment with the mTOR inhibitor rapamycin, decreased carbachol-induced Ca(2+) release in AR4-2J cells. Carbachol 58-67 mechanistic target of rapamycin kinase Rattus norvegicus 22-26 20727967-6 2011 We also showed that IGF-1 potentiates carbachol-induced Ca(2+) release in AR4-2J cells, an effect that was prevented by rapamycin. Carbachol 38-47 insulin-like growth factor 1 Rattus norvegicus 20-25 20727967-7 2011 Rapamycin also decreased carbachol-induced Ca(2+) release in HEK 293A cells in which IP(3)R-1 and IP(3)R-3 had been knocked down. Carbachol 25-34 inositol 1,4,5-trisphosphate receptor type 1 Homo sapiens 85-93 20727967-7 2011 Rapamycin also decreased carbachol-induced Ca(2+) release in HEK 293A cells in which IP(3)R-1 and IP(3)R-3 had been knocked down. Carbachol 25-34 inositol 1,4,5-trisphosphate receptor type 3 Homo sapiens 98-106 21221778-4 2011 This study shows that treatment of the wild type HEK293 cells with low concentrations of carbachol (1-10 muM), an agonist of the muscarinic receptor, resulted in non-oscillated but sustained [Ca(2+)](i) increase by loading the cells with 1 muM fura2/AM. Carbachol 89-98 latexin Homo sapiens 105-108 21221778-4 2011 This study shows that treatment of the wild type HEK293 cells with low concentrations of carbachol (1-10 muM), an agonist of the muscarinic receptor, resulted in non-oscillated but sustained [Ca(2+)](i) increase by loading the cells with 1 muM fura2/AM. Carbachol 89-98 latexin Homo sapiens 240-243 20851763-4 2011 Using serum starved SK-N-SH neuroblastoma cells, we show that the muscarinic receptor agonists carbachol and pilocarpine enhance the activation of the mTOR substrate p70 S6 Kinase (S6K) and its target ribosomal protein S6 (S6) in a VEGFR2 dependent manner. Carbachol 95-104 ribosomal protein S6 kinase B1 Homo sapiens 181-184 20851763-4 2011 Using serum starved SK-N-SH neuroblastoma cells, we show that the muscarinic receptor agonists carbachol and pilocarpine enhance the activation of the mTOR substrate p70 S6 Kinase (S6K) and its target ribosomal protein S6 (S6) in a VEGFR2 dependent manner. Carbachol 95-104 ribosomal protein S6 Homo sapiens 201-221 20851763-4 2011 Using serum starved SK-N-SH neuroblastoma cells, we show that the muscarinic receptor agonists carbachol and pilocarpine enhance the activation of the mTOR substrate p70 S6 Kinase (S6K) and its target ribosomal protein S6 (S6) in a VEGFR2 dependent manner. Carbachol 95-104 kinase insert domain receptor Homo sapiens 232-238 20851763-5 2011 Treatments with carbachol increased VEGFR2 phosphorylation, suggesting that mAchRs stimulate VEGFR2 transactivation to enhance mTOR signaling. Carbachol 16-25 kinase insert domain receptor Homo sapiens 36-42 20851763-5 2011 Treatments with carbachol increased VEGFR2 phosphorylation, suggesting that mAchRs stimulate VEGFR2 transactivation to enhance mTOR signaling. Carbachol 16-25 kinase insert domain receptor Homo sapiens 93-99 20851763-5 2011 Treatments with carbachol increased VEGFR2 phosphorylation, suggesting that mAchRs stimulate VEGFR2 transactivation to enhance mTOR signaling. Carbachol 16-25 mechanistic target of rapamycin kinase Homo sapiens 127-131 21865666-8 2011 Similarly, EE increased phospholipase C activity in Ts65Dn mice, in response to carbachol and calcium. Carbachol 80-89 reciprocal translocation, Chr 16, cytogenetic band C3-4; and Chr 17, cytogenetic band A2, Davisson 65 Mus musculus 52-58 20658540-2 2011 Recently, we showed that carbachol, an acetylcholine analog, stimulates contraction of C2C12 myotube cultures and the rapid arrival of myc-epitope tagged GLUT4 glucose transporters at the cell surface. Carbachol 25-34 solute carrier family 2 member 4 Homo sapiens 154-159 20658540-4 2011 Cell surface carbachol-induced GLUT4myc levels were partly inhibited by the conventional/novel PKC inhibitors GF-109203X, Go6983, and Ro-31-8425 but not by the conventional PKC inhibitor Go6976. Carbachol 13-22 solute carrier family 2 member 4 Homo sapiens 31-36 20658540-4 2011 Cell surface carbachol-induced GLUT4myc levels were partly inhibited by the conventional/novel PKC inhibitors GF-109203X, Go6983, and Ro-31-8425 but not by the conventional PKC inhibitor Go6976. Carbachol 13-22 protein kinase C delta Homo sapiens 95-98 20658540-6 2011 Carbachol stimulated phosphorylation of PKC isoforms and translocation of PKCdelta and PKCepsilon to membranes within 5 min. Carbachol 0-9 protein kinase C delta Homo sapiens 40-43 20658540-6 2011 Carbachol stimulated phosphorylation of PKC isoforms and translocation of PKCdelta and PKCepsilon to membranes within 5 min. Carbachol 0-9 protein kinase C delta Homo sapiens 74-82 20658540-6 2011 Carbachol stimulated phosphorylation of PKC isoforms and translocation of PKCdelta and PKCepsilon to membranes within 5 min. Carbachol 0-9 protein kinase C epsilon Homo sapiens 87-97 20658540-7 2011 However, only a peptidic inhibitor of PKCepsilon translocation (myristoylated-EAVSLKPT), but not one of PKCdelta (myristoylated-SFNSYELGSL), prevented the GLUT4myc response to carbachol. Carbachol 176-185 solute carrier family 2 member 4 Homo sapiens 155-160 20658540-8 2011 Significant participation of PKCepsilon in the carbachol-induced gain of GLUT4myc at the surface of C2C12 myotubes was further supported through siRNA-mediated PKCepsilon protein knockdown. Carbachol 47-56 solute carrier family 2 member 4 Homo sapiens 73-78 20658540-8 2011 Significant participation of PKCepsilon in the carbachol-induced gain of GLUT4myc at the surface of C2C12 myotubes was further supported through siRNA-mediated PKCepsilon protein knockdown. Carbachol 47-56 protein kinase C epsilon Homo sapiens 29-39 20961851-6 2010 Activation of the cells with carbachol increased the phosphorylation of TRPC6, an effect that was prevented by the inhibition of PKC. Carbachol 29-38 transient receptor potential cation channel, subfamily C, member 6 Rattus norvegicus 72-77 20933560-7 2011 GLP-1 was without any effect in fundic strips, but it induced concentration-dependent relaxation in carbachol-precontracted antral strips. Carbachol 100-109 glucagon Mus musculus 0-5 21966394-13 2011 Interestingly, at the cellular signalling level, phosphorylation assays revealed abolished carbachol-triggered activation of ERK1/2 in mice lacking Galpha(i2). Carbachol 91-100 mitogen-activated protein kinase 3 Mus musculus 125-131 21966394-13 2011 Interestingly, at the cellular signalling level, phosphorylation assays revealed abolished carbachol-triggered activation of ERK1/2 in mice lacking Galpha(i2). Carbachol 91-100 guanine nucleotide binding protein (G protein), alpha inhibiting 2 Mus musculus 148-157 20961851-6 2010 Activation of the cells with carbachol increased the phosphorylation of TRPC6, an effect that was prevented by the inhibition of PKC. Carbachol 29-38 protein kinase C, gamma Rattus norvegicus 129-132 21203481-4 2010 Here, this principle is demonstrated for analysis of a G-protein coupled receptor system, the M3 muscarinic receptor-calcium signaling pathway, using microfluidic-mediated periodic chemical stimulation of the M3 receptor with carbachol and real-time imaging of resulting calcium transients. Carbachol 226-235 cholinergic receptor muscarinic 3 Homo sapiens 94-116 20816837-9 2010 Based on previous reports that Ca(2+)(i) release also stimulates acid secretion in parietal cells, we showed that gadolinium-, thapsigargin-, and carbachol-mediated release of Ca(2+)(i) induced Shh expression. Carbachol 146-155 sonic hedgehog Mus musculus 194-197 20671279-10 2010 Incubation with dbcAMP did not have any effect either on the EGF receptor or on Ras but significantly inhibited both basal and Raf-1 and MEK activity stimulated with Cch or EGF. Carbachol 166-169 Raf-1 proto-oncogene, serine/threonine kinase Rattus norvegicus 127-132 20863867-5 2010 The concentration-contraction curves to carbachol (CCh) were shifted to the left in the presence of Ang II. Carbachol 40-49 angiotensinogen Rattus norvegicus 100-106 20938046-5 2010 Application of the muscarinic agonist carbachol to the cells caused a rapid, time-dependent decrease in the fluorescent signal, indicative of K(+) efflux through the GIRK1/4 channel (carbachol vs. control solution, Z" factor = 0.5-0.6). Carbachol 38-47 potassium inwardly-rectifying channel, subfamily J, member 3 Mus musculus 166-171 20938046-5 2010 Application of the muscarinic agonist carbachol to the cells caused a rapid, time-dependent decrease in the fluorescent signal, indicative of K(+) efflux through the GIRK1/4 channel (carbachol vs. control solution, Z" factor = 0.5-0.6). Carbachol 183-192 potassium inwardly-rectifying channel, subfamily J, member 3 Mus musculus 166-171 20863867-5 2010 The concentration-contraction curves to carbachol (CCh) were shifted to the left in the presence of Ang II. Carbachol 51-54 angiotensinogen Rattus norvegicus 100-106 20863867-6 2010 The maximal contraction of CCh was also significantly increased by pretreatment with Ang II. Carbachol 27-30 angiotensinogen Rattus norvegicus 85-91 20976005-5 2010 Treatment of cells with the agonist carbachol disrupted the interaction of RACK1 with M(2). Carbachol 36-45 receptor for activated C kinase 1 Homo sapiens 75-80 20805306-3 2010 In the guinea pig isolated trachea and human isolated bronchus, CHF5407 produced a potent (pIC(50) = 9.0-9.6) and long-lasting (up to 24 h) inhibition of M3 receptor-mediated contractile responses to carbachol. Carbachol 200-209 cholinergic receptor muscarinic 3 Homo sapiens 154-156 20805306-4 2010 In the guinea pig electrically driven left atrium, the M2 receptor-mediated inhibitory response to carbachol was recovered more quickly in CHF5407-pretreated than in tiotropium-pretreated preparations. Carbachol 99-108 cholinergic receptor muscarinic 2 Homo sapiens 55-57 20864673-7 2010 Loss of RGS6 provoked dramatically exaggerated bradycardia in response to carbachol in mice and isolated perfused hearts and significantly enhanced the effect of carbachol on inhibition of spontaneous action potential firing in sinoatrial node cells. Carbachol 74-83 regulator of G-protein signaling 6 Mus musculus 8-12 20864673-7 2010 Loss of RGS6 provoked dramatically exaggerated bradycardia in response to carbachol in mice and isolated perfused hearts and significantly enhanced the effect of carbachol on inhibition of spontaneous action potential firing in sinoatrial node cells. Carbachol 162-171 regulator of G-protein signaling 6 Mus musculus 8-12 20876203-11 2010 Following TTX application, carbachol (1 muM), substance P (1 muM) and an NKI agonist (GR73632, 100 nM) produced the fast oscillations superimposed on a slow increase in Ca(2+) in ICC-MY, whereas SNP (an NO donor, 10 muM) abolished all activity in ICC-MY. Carbachol 27-36 latexin Homo sapiens 40-43 20413533-0 2010 FIZZ1 potentiates the carbachol-induced tracheal smooth muscle contraction. Carbachol 22-31 resistin like alpha Mus musculus 0-5 20495491-3 2010 RESULTS: The results indicated that PKCalpha and epsilon agonists, thymelea toxin and carbachol, restored shock-induced decrease of vascular calcium sensitivity; PKCalpha antagonist, Go-6976 and PKCepsilon pseudosubstrate inhibition peptide, aggravated shock-induced calcium desensitization, whereas the agonists and antagonists of PKCdelta and zeta had no effects on shock-induced calcium desensitization. Carbachol 86-95 protein kinase C, alpha Rattus norvegicus 36-44 20495491-3 2010 RESULTS: The results indicated that PKCalpha and epsilon agonists, thymelea toxin and carbachol, restored shock-induced decrease of vascular calcium sensitivity; PKCalpha antagonist, Go-6976 and PKCepsilon pseudosubstrate inhibition peptide, aggravated shock-induced calcium desensitization, whereas the agonists and antagonists of PKCdelta and zeta had no effects on shock-induced calcium desensitization. Carbachol 86-95 protein kinase C, alpha Rattus norvegicus 162-170 20648635-4 2010 As M(3) muscarinic receptor signaling in astroglial cells is strongly inhibited by ethanol, we hypothesized that ethanol may also inhibit neuritogenesis in hippocampal neurons induced by carbachol-stimulated astrocytes. Carbachol 187-196 cholinergic receptor muscarinic 3 Homo sapiens 3-27 20573995-6 2010 Only knockdown of RGS11 increased both carbachol-mediated calcium mobilization and inositol phosphate accumulation. Carbachol 39-48 regulator of G protein signaling 11 Homo sapiens 18-23 20573995-7 2010 Surprisingly, we found that knockdown of RGS8 and RGS9, but not other conventional RGS proteins, significantly decreased carbachol-mediated calcium mobilization, whereas only RGS8 knockdown decreased protease-activated receptor-1 (PAR-1)-mediated calcium mobilization. Carbachol 121-130 regulator of G protein signaling 8 Homo sapiens 41-45 20573995-7 2010 Surprisingly, we found that knockdown of RGS8 and RGS9, but not other conventional RGS proteins, significantly decreased carbachol-mediated calcium mobilization, whereas only RGS8 knockdown decreased protease-activated receptor-1 (PAR-1)-mediated calcium mobilization. Carbachol 121-130 regulator of G protein signaling 9 Homo sapiens 50-54 20573995-7 2010 Surprisingly, we found that knockdown of RGS8 and RGS9, but not other conventional RGS proteins, significantly decreased carbachol-mediated calcium mobilization, whereas only RGS8 knockdown decreased protease-activated receptor-1 (PAR-1)-mediated calcium mobilization. Carbachol 121-130 paired like homeodomain 2 Homo sapiens 41-44 20573995-8 2010 Loss of responsiveness toward carbachol and PAR-1 agonist peptide upon RGS8 knockdown appears due, at least in part, to a loss in respective receptor cell surface expression, although this is not the case for RGS9 knockdown. Carbachol 30-39 regulator of G protein signaling 8 Homo sapiens 71-75 20573995-9 2010 Our data suggest a cellular role for RGS8 in the stable surface expression of M3 muscarinic acetylcholine receptor and PAR-1, as well as a specific and opposing set of functions for RGS9 and RGS11 in modulating carbachol responsiveness similar to that seen in Caenorhabditis elegans. Carbachol 211-220 Regulator of G-protein signaling rgs-9 Caenorhabditis elegans 182-186 20573995-9 2010 Our data suggest a cellular role for RGS8 in the stable surface expression of M3 muscarinic acetylcholine receptor and PAR-1, as well as a specific and opposing set of functions for RGS9 and RGS11 in modulating carbachol responsiveness similar to that seen in Caenorhabditis elegans. Carbachol 211-220 Regulator of G-protein signaling rgs-11 Caenorhabditis elegans 191-196 20375347-0 2010 Mechanism for carbachol-induced secretion of lacritin in cultured monkey lacrimal acinar cells. Carbachol 14-23 lacritin Homo sapiens 45-53 20375347-10 2010 The cholinergic agonist carbachol (Cch) stimulated the secretion of lacritin and increased intracellular Ca2+. Carbachol 24-33 lacritin Homo sapiens 68-76 20375347-10 2010 The cholinergic agonist carbachol (Cch) stimulated the secretion of lacritin and increased intracellular Ca2+. Carbachol 35-38 lacritin Homo sapiens 68-76 20375347-11 2010 Cch-induced lacritin secretion was inhibited by the store-operated calcium (SOC) channel inhibitor YM58483 and the PKC inhibitors GF109203 and Ro-32-0432. Carbachol 0-3 lacritin Homo sapiens 12-20 20375347-12 2010 Cch-induced lacritin secretion was not inhibited by MAPKK inhibitor U0126, although p42/p44 MAPK was phosphorylated. Carbachol 0-3 lacritin Homo sapiens 12-20 20375347-12 2010 Cch-induced lacritin secretion was not inhibited by MAPKK inhibitor U0126, although p42/p44 MAPK was phosphorylated. Carbachol 0-3 cyclin dependent kinase 20 Homo sapiens 84-87 20375347-12 2010 Cch-induced lacritin secretion was not inhibited by MAPKK inhibitor U0126, although p42/p44 MAPK was phosphorylated. Carbachol 0-3 mitogen-activated protein kinase 3 Homo sapiens 88-96 20375347-15 2010 Induction of transcription by Cch involved the independent p42/p44 MAPK and PKC pathways. Carbachol 30-33 cyclin dependent kinase 20 Homo sapiens 59-62 20375347-15 2010 Induction of transcription by Cch involved the independent p42/p44 MAPK and PKC pathways. Carbachol 30-33 interferon induced protein 44 Homo sapiens 63-66 20375347-15 2010 Induction of transcription by Cch involved the independent p42/p44 MAPK and PKC pathways. Carbachol 30-33 mitogen-activated protein kinase 3 Homo sapiens 67-71 20871620-0 2010 Carbachol inhibits TNF-alpha-induced endothelial barrier dysfunction through alpha 7 nicotinic receptors. Carbachol 0-9 tumor necrosis factor Rattus norvegicus 19-28 20871620-6 2010 RESULTS: Carbachol (2 mumol/L-2 mmol/L) prevented increase in endothelial cell permeability induced by TNF-alpha (500 ng/mL) in a dose-dependent manner. Carbachol 9-18 tumor necrosis factor Rattus norvegicus 103-112 20871620-8 2010 In addition, treatment of endothelial cells with carbachol decreased phosphor-ERK1/2 and phosphor-JNK. Carbachol 49-58 mitogen activated protein kinase 3 Rattus norvegicus 78-84 20871620-8 2010 In addition, treatment of endothelial cells with carbachol decreased phosphor-ERK1/2 and phosphor-JNK. Carbachol 49-58 mitogen-activated protein kinase 8 Rattus norvegicus 98-101 20871620-10 2010 CONCLUSION: These data suggest that the inhibitory effect of carbachol on TNF-alpha-induced endothelial barrier dysfunction mediated by the alpha 7 nicotinic receptor. Carbachol 61-70 tumor necrosis factor Rattus norvegicus 74-83 20689057-7 2010 As a functional consequence, loss of PTPN2 potentiated EGF-induced inhibition of carbachol-stimulated Ca(2+)-dependent Cl(-) secretion. Carbachol 81-90 protein tyrosine phosphatase non-receptor type 2 Homo sapiens 37-42 20693290-11 2010 Carbachol increased the probability of SR input to drive action potential firing in CA1 pyramidal neurons, which was inhibited by SLM prepulses (150-225 ms). Carbachol 0-9 carbonic anhydrase 1 Rattus norvegicus 84-87 19783789-9 2010 In vitro incubation with IL-13, but not TGF-beta(1), induced changes in small airway sensitivity to CCh and 5HT. Carbachol 100-103 interleukin 13 Rattus norvegicus 25-30 20643770-9 2010 ATP and carbachol increased intracellular Ca(2+) activity, but responses depended on the gland type, presence of the P2X(7) receptor and the sex of the animal. Carbachol 8-17 purinergic receptor P2X, ligand-gated ion channel, 7 Mus musculus 117-132 20505521-8 2010 Pioglitazone (PPAR gamma agonist, 10 microM) abolished the CSA-induced attenuation of carbachol responses, an effect that was not manifest in presence of GW9662 or l-NAME. Carbachol 86-95 peroxisome proliferator-activated receptor gamma Rattus norvegicus 14-24 20505521-10 2010 More importantly, NOS signaling downstream of PPAR gamma mediates, at least partly, the inhibitory effect of CSA on carbachol vasodilations. Carbachol 116-125 peroxisome proliferator-activated receptor gamma Rattus norvegicus 46-56 20547680-6 2010 Intriguingly, however, we found that bath application of the GAT-1 transport blocker NO-711 (1 mum) produces inhibition of evoked IPSCs that is reversed by CGP52432, and that lower doses of CCh produce inhibition with greater CGP52432 sensitivity. Carbachol 190-193 solute carrier family 6 member 12 Rattus norvegicus 61-66 20495822-5 2010 The contractions induced by carbachol (CCh), high K(+) depolarization, and sodium fluoride (NaF; a G protein activator) were augmented by pretreatment with Ang II. Carbachol 28-37 angiotensinogen Rattus norvegicus 156-162 20495822-5 2010 The contractions induced by carbachol (CCh), high K(+) depolarization, and sodium fluoride (NaF; a G protein activator) were augmented by pretreatment with Ang II. Carbachol 39-42 angiotensinogen Rattus norvegicus 156-162 20495822-7 2010 Furthermore, the Ang II-induced BSM hyperresponsiveness to CCh was attenuated by pretreatment with U-0126, a p42/44 ERK kinase (MEK-1/2) inhibitor. Carbachol 59-62 angiotensinogen Rattus norvegicus 17-23 20495822-7 2010 Furthermore, the Ang II-induced BSM hyperresponsiveness to CCh was attenuated by pretreatment with U-0126, a p42/44 ERK kinase (MEK-1/2) inhibitor. Carbachol 59-62 Eph receptor B1 Rattus norvegicus 116-119 20495822-7 2010 Furthermore, the Ang II-induced BSM hyperresponsiveness to CCh was attenuated by pretreatment with U-0126, a p42/44 ERK kinase (MEK-1/2) inhibitor. Carbachol 59-62 mitogen activated protein kinase kinase 1 Rattus norvegicus 128-135 20398705-5 2010 In addition, the same peptide was able to abrogate the carbachol-induced mitogen-activated protein kinase phosphorylation and the carbachol-enhanced PHA-induced IL-2 production in derived lymphocytic T cells. Carbachol 130-139 interleukin 2 Homo sapiens 161-165 19655318-3 2010 Interestingly, such neural responses in CA1 are also elicited by microinjection of the cholinergic agonist carbachol into the hypothalamic supramammillary nucleus (SuM). Carbachol 107-116 carbonic anhydrase 1 Rattus norvegicus 40-43 19655318-5 2010 Pharmacological investigation showed that intra-SuM microinjection of either a muscarinic or a nicotinic receptor antagonist attenuated the SuM carbachol-induced neural effects in CA1, namely, theta activation and PS suppression. Carbachol 144-153 carbonic anhydrase 1 Rattus norvegicus 180-183 20739756-8 2010 First, CFTR-dependent secretion was strongly potentiated by low VIP and carbachol concentrations that individually were unable to stimulate secretion. Carbachol 72-81 CF transmembrane conductance regulator Homo sapiens 7-11 20371628-7 2010 Both carbachol and PDBu increased Ca(V)1.2 channel currents in isolated bladder myocytes. Carbachol 5-14 calcium channel, voltage-dependent, L type, alpha 1C subunit Mus musculus 34-42 20371628-8 2010 Blue native-PAGE electrophoresis revealed that Ca(V)1.2, PKC, and PLD are closely associated in muscles being previously stimulated by carbachol. Carbachol 135-144 calcium channel, voltage-dependent, L type, alpha 1C subunit Mus musculus 47-55 20505521-6 2010 The carbachol-induced renal vasodilations were also reduced after infusion of N-nitro-L-arginine methyl ester (L-NAME, NOS inhibitor, 100 microM) or 2-chloro-5-nitro-N-phenylbenzamide (GW9662, PPAR gamma antagonist, 1 microM). Carbachol 4-13 peroxisome proliferator-activated receptor gamma Rattus norvegicus 193-203 20505129-0 2010 Carbachol-induced long-term synaptic depression is enhanced during senescence at hippocampal CA3-CA1 synapses. Carbachol 0-9 carbonic anhydrase 3 Rattus norvegicus 93-100 20478977-3 2010 Prostaglandin E(2) (PGE(2))- and carbachol-stimulated mucus release was severely inhibited in the absence of serosal HCO(3)(-), HCO(3)(-) transport, or functional cystic fibrosis transmembrane conductance regulator (CFTR). Carbachol 33-42 CF transmembrane conductance regulator Homo sapiens 163-214 20478977-3 2010 Prostaglandin E(2) (PGE(2))- and carbachol-stimulated mucus release was severely inhibited in the absence of serosal HCO(3)(-), HCO(3)(-) transport, or functional cystic fibrosis transmembrane conductance regulator (CFTR). Carbachol 33-42 CF transmembrane conductance regulator Homo sapiens 216-220 20421298-11 2010 Consistent with this, carbachol potentiated GIP-mediated insulin release from in situ perfused pancreata of GIP/DT mice. Carbachol 22-31 gastric inhibitory polypeptide Mus musculus 44-47 20592220-8 2010 In mice with recurrent seizures, carbachol-induced enhancement of spontaneous IPSCs (sIPSCs) originating from CCK-containing basket cells was accordingly reduced in CA1 pyramidal cells. Carbachol 33-42 cholecystokinin Mus musculus 110-113 20592220-8 2010 In mice with recurrent seizures, carbachol-induced enhancement of spontaneous IPSCs (sIPSCs) originating from CCK-containing basket cells was accordingly reduced in CA1 pyramidal cells. Carbachol 33-42 carbonic anhydrase 1 Mus musculus 165-168 20592220-9 2010 By suppressing sIPSCs from CCK-expressing basket cells, a CB(1) agonist reverted the stimulatory effects of carbachol in naive mice to levels comparable with those observed in cells from epileptic mice. Carbachol 108-117 cholecystokinin Mus musculus 27-30 20592220-9 2010 By suppressing sIPSCs from CCK-expressing basket cells, a CB(1) agonist reverted the stimulatory effects of carbachol in naive mice to levels comparable with those observed in cells from epileptic mice. Carbachol 108-117 cannabinoid receptor 1 (brain) Mus musculus 58-63 21117404-6 2010 PKG also decreased stimulated NO production: acetylcholine produced raw fluorescence of 59999 +/- 702 and in the presence of 1 mM 8-Br-cGMP the value was 20645 +/- 292 (p < 0.0001) while carbachol produced raw fluorescence of 60600 +/- 890 and in the presence of 1 mM 8-Br-cGMP was 30442 +/- 2000 (p < 0.01). Carbachol 190-199 protein kinase cGMP-dependent 1 Homo sapiens 0-3 20421298-11 2010 Consistent with this, carbachol potentiated GIP-mediated insulin release from in situ perfused pancreata of GIP/DT mice. Carbachol 22-31 gastric inhibitory polypeptide Mus musculus 108-111 20572856-6 2010 Inhibition of TRPV4 with ruthenium red impaired both ATP- and carbachol-stimulated Ca(2+) signals. Carbachol 62-71 transient receptor potential cation channel, subfamily V, member 4 Rattus norvegicus 14-19 20572856-9 2010 Furthermore, even though Ca(2+) signals were not needed for this volume regulation, TRPV4 seems to play a role during ATP and carbachol stimulation. Carbachol 126-135 transient receptor potential cation channel, subfamily V, member 4 Rattus norvegicus 84-89 20390293-5 2010 TRPC5 activity could be evoked by carbachol acting at muscarinic receptors, lanthanum, or a reducing agent. Carbachol 34-43 transient receptor potential cation channel subfamily C member 5 Homo sapiens 0-5 20159855-3 2010 Carbachol (an acetylcholine receptor agonist) induced a gain in cell surface GLUT4myc that was mediated by nicotinic acetylcholine receptors. Carbachol 0-9 solute carrier family 2 member 4 Homo sapiens 77-82 20159855-5 2010 The gain in surface GLUT4myc elicited by carbachol or by the AMPK activator 5-amino-4-carboxamide-1 beta-ribose was sensitive to chemical inhibition of AMPK activity by compound C and partially reduced by siRNA-mediated knockdown of AMPK catalytic subunits or LKB1. Carbachol 41-50 solute carrier family 2 member 4 Homo sapiens 20-25 20159855-5 2010 The gain in surface GLUT4myc elicited by carbachol or by the AMPK activator 5-amino-4-carboxamide-1 beta-ribose was sensitive to chemical inhibition of AMPK activity by compound C and partially reduced by siRNA-mediated knockdown of AMPK catalytic subunits or LKB1. Carbachol 41-50 serine/threonine kinase 11 Homo sapiens 260-264 20159855-6 2010 In addition, the carbachol-induced gain in cell surface GLUT4myc was partially sensitive to chelation of intracellular calcium with BAPTA-AM. Carbachol 17-26 solute carrier family 2 member 4 Homo sapiens 56-61 20159855-7 2010 However, the carbachol-induced gain in cell surface GLUT4myc was not sensitive to the CaMKK inhibitor STO-609 despite expression of both isoforms of this enzyme and a rise in cytosolic calcium by carbachol. Carbachol 13-22 solute carrier family 2 member 4 Homo sapiens 52-57 20159855-8 2010 Therefore, separate AMPK- and calcium-dependent signals contribute to mobilizing GLUT4 in response to carbachol, providing an in vitro cell model that recapitulates the two major signals whereby acute contraction regulates glucose uptake in skeletal muscle. Carbachol 102-111 solute carrier family 2 member 4 Homo sapiens 81-86 20203064-7 2010 Moreover, pretreatment of T(84) cells with leptin for up to 1 h significantly potentiated carbachol- and forskolin-induced increases in I(sc). Carbachol 90-99 leptin Rattus norvegicus 43-49 20203064-8 2010 Pretreatment with an inhibitor of MAPK abolished the effect of leptin on basal, carbachol- and forskolin-induced chloride secretion (P < 0.05). Carbachol 80-89 leptin Rattus norvegicus 63-69 20233329-10 2010 Furthermore, carbachol directly increased the mRNA expression of AQP5 and phosphorylation of ERK1/2 in cultured neonatal rabbit SMG cells. Carbachol 13-22 aquaporin-5 Oryctolagus cuniculus 65-69 20229195-10 2010 Twenty-minute incubation with hBD-2 increased the CCh-induced Ca(2+) transient by 20-30% compared to either vehicle-treated cells or cells treated with the defensins hBD-1, hBD-3, or HD-5. Carbachol 50-53 defensin beta 4A Homo sapiens 30-35 20229195-10 2010 Twenty-minute incubation with hBD-2 increased the CCh-induced Ca(2+) transient by 20-30% compared to either vehicle-treated cells or cells treated with the defensins hBD-1, hBD-3, or HD-5. Carbachol 50-53 defensin beta 1 Homo sapiens 166-171 20229195-10 2010 Twenty-minute incubation with hBD-2 increased the CCh-induced Ca(2+) transient by 20-30% compared to either vehicle-treated cells or cells treated with the defensins hBD-1, hBD-3, or HD-5. Carbachol 50-53 defensin beta 103B Homo sapiens 173-178 20138847-3 2010 One such mouse is the GAD67-GFP (Deltaneo), and here we compare the properties of kainate- and carbachol-induced oscillatory activity generated in CA3 of hippocampal slices from heterozygous GAD67-GFP (Deltaneo) mice and wild type litter mates. Carbachol 95-104 carbonic anhydrase 3 Mus musculus 147-150 20061444-3 2010 We have investigated the relaxation of carbachol-contracted mouse tracheal segments to the beta(2)AR agonists formoterol, terbutaline, and salmeterol. Carbachol 39-48 adrenergic receptor, beta 2 Mus musculus 91-100 20399743-5 2010 In these cells optimal activation of endogenous Galphaq by expressing M3-muscarinic acetylcholine receptor (with or without carbachol treatment), or exposing the cells to thrombin led to an increase of 2 to 3-fold in endogenous cytoplasmic beta-Catenin protein levels. Carbachol 124-133 coagulation factor II, thrombin Homo sapiens 171-179 20543478-1 2010 OBJECTIVE: To investigate the regulation of atropine to the expression and secretion of TGF-beta2 in retinal pigment epithelium (RPE) cells by observing the changes of those under different treatments of atropine and carbachol. Carbachol 217-226 transforming growth factor beta 2 Homo sapiens 88-97 20543478-9 2010 CONCLUSION: Carbachol can promote the expression and secretion of TGF-beta2 in human RPE cells and atropine could reverse it effectively, suggesting that M receptor may be involved. Carbachol 12-21 transforming growth factor beta 2 Homo sapiens 66-75 20207737-3 2010 Here we report that the beta-adrenergic ligand isoproterenol blocks increases in extracellular signal-related kinase (ERK) phosphorylation, a protein kinase C-dependent event promoted by the muscarinic receptor ligand carbachol in freshly dispersed rat parotid acinar cells. Carbachol 218-227 Eph receptor B1 Rattus norvegicus 81-116 20207737-3 2010 Here we report that the beta-adrenergic ligand isoproterenol blocks increases in extracellular signal-related kinase (ERK) phosphorylation, a protein kinase C-dependent event promoted by the muscarinic receptor ligand carbachol in freshly dispersed rat parotid acinar cells. Carbachol 218-227 Eph receptor B1 Rattus norvegicus 118-121 20172859-5 2010 Elevation of intracellular calcium by ionomycin treatment, or activation of acetylcholine receptor or epidermal growth factor receptor by carbachol or epidermal growth factor stimulation induced activation of endogenous Nedd4 in vivo evaluated by assays of either Nedd4 E3 ligase activity or ubiquitination of Nedd4 substrate ENaC-beta. Carbachol 138-147 NEDD4 E3 ubiquitin protein ligase Homo sapiens 220-225 19666830-5 2010 Activation of phospholipase C (PLC), protein kinase C (PKC), and postsynaptic Ca(2+) release from inositol 1,4,5-triphosphate (IP(3)) receptor-sensitive internal stores are required for CCh-LTD induction. Carbachol 186-189 inositol 1,4,5-trisphosphate receptor, type 3 Rattus norvegicus 127-142 19666830-7 2010 CCh-LTD is blocked by nitric oxide (NO) synthase inhibitors, soluble guanylyl cyclase (sGC) inhibitor, and protein kinase G (PKG) inhibitor. Carbachol 0-3 guanylate cyclase 1 soluble subunit alpha 1 Rattus norvegicus 61-85 19666830-7 2010 CCh-LTD is blocked by nitric oxide (NO) synthase inhibitors, soluble guanylyl cyclase (sGC) inhibitor, and protein kinase G (PKG) inhibitor. Carbachol 0-3 guanylate cyclase 1 soluble subunit alpha 1 Rattus norvegicus 87-90 20012300-0 2010 Carbachol induces TGF-alpha expression and colonic epithelial cell proliferation in sensory-desensitised rats. Carbachol 0-9 transforming growth factor alpha Rattus norvegicus 18-27 20234002-3 2010 We found that ankyrin-B-deficient islets displayed impaired potentiation of insulin secretion by the muscarinic agonist carbachol, blunted carbachol-mediated intracellular Ca2+ release, and reduced the abundance of IP3R. Carbachol 120-129 ankyrin 2, brain Mus musculus 14-23 20234002-3 2010 We found that ankyrin-B-deficient islets displayed impaired potentiation of insulin secretion by the muscarinic agonist carbachol, blunted carbachol-mediated intracellular Ca2+ release, and reduced the abundance of IP3R. Carbachol 139-148 ankyrin 2, brain Mus musculus 14-23 20012300-3 2010 The aim of our present study was to determine the effects of carbachol on mucosal TGFalpha expression and epithelial cell proliferation in vivo. Carbachol 61-70 transforming growth factor alpha Rattus norvegicus 82-90 20012300-7 2010 RESULTS: Carbachol induced a significant increase in mucosal epithelial cell proliferation and TGFalpha expression. Carbachol 9-18 transforming growth factor alpha Rattus norvegicus 95-103 20136837-8 2010 The rate of NTUA accumulation was slower in the presence of the muscarinic agonist carbachol (10 microM) demonstrating muscarinic inhibition of NET rate. Carbachol 83-92 solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2 Mus musculus 144-147 19923416-9 2010 Carbachol increased RBF and decreased MAP in SMTC-treated rats, whereas it had no effect in L-NAME-treated rats, indicating that SMTC selectively inhibited NOS1. Carbachol 0-9 nitric oxide synthase 1 Rattus norvegicus 156-160 19823767-2 2010 In this work, we investigate the action of the muscarinic agonist carbachol (CARB) on the expression and function of nitric oxide synthase (NOS) and cyclooxygenase (COX) in fibroblasts under normal or inflammatory conditions. Carbachol 66-75 nitric oxide synthase 1, neuronal Mus musculus 117-138 19823767-2 2010 In this work, we investigate the action of the muscarinic agonist carbachol (CARB) on the expression and function of nitric oxide synthase (NOS) and cyclooxygenase (COX) in fibroblasts under normal or inflammatory conditions. Carbachol 77-81 nitric oxide synthase 1, neuronal Mus musculus 117-138 19823767-9 2010 CARB also upregulated NOS1 protein expression via NF-kappaB activation. Carbachol 0-4 nitric oxide synthase 1, neuronal Mus musculus 22-26 19823767-9 2010 CARB also upregulated NOS1 protein expression via NF-kappaB activation. Carbachol 0-4 nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 Mus musculus 50-59 19823767-10 2010 In addition CARB and LPS plus IFNgamma stimulated PGE(2) synthesis by 72 +/- 9 and 42 +/- 4%, respectively, while CARB added to LPS plus IFNgamma treated cells produced a synergism in PGE(2) liberation (130 +/- 12%) via COX-2. Carbachol 114-118 cytochrome c oxidase II, mitochondrial Mus musculus 220-225 19763792-1 2010 Previous studies on MCF-7 breast cancer cells have shown that the G-protein coupled receptor (GPCR) agonist carbachol increases intracellular calcium levels and the activation of extracellular signal-regulated kinase (ERK). Carbachol 108-117 C-X-C motif chemokine receptor 6 Homo sapiens 66-92 19763792-1 2010 Previous studies on MCF-7 breast cancer cells have shown that the G-protein coupled receptor (GPCR) agonist carbachol increases intracellular calcium levels and the activation of extracellular signal-regulated kinase (ERK). Carbachol 108-117 C-X-C motif chemokine receptor 6 Homo sapiens 94-98 19763792-1 2010 Previous studies on MCF-7 breast cancer cells have shown that the G-protein coupled receptor (GPCR) agonist carbachol increases intracellular calcium levels and the activation of extracellular signal-regulated kinase (ERK). Carbachol 108-117 mitogen-activated protein kinase 1 Homo sapiens 179-216 19763792-1 2010 Previous studies on MCF-7 breast cancer cells have shown that the G-protein coupled receptor (GPCR) agonist carbachol increases intracellular calcium levels and the activation of extracellular signal-regulated kinase (ERK). Carbachol 108-117 mitogen-activated protein kinase 1 Homo sapiens 218-221 19763792-3 2010 Our results suggest that both estrogen (E2) and carbachol treatment of MCF-7 breast cancer cells trigger phosphorylation of ERK1/2 and the transcription factor Elk-1. Carbachol 48-57 mitogen-activated protein kinase 3 Homo sapiens 124-130 19763792-3 2010 Our results suggest that both estrogen (E2) and carbachol treatment of MCF-7 breast cancer cells trigger phosphorylation of ERK1/2 and the transcription factor Elk-1. Carbachol 48-57 ETS transcription factor ELK1 Homo sapiens 160-165 19763792-5 2010 Carbachol-stimulated ERK activation and cell growth was completely blocked by the Muscarinic M(3)-subtype GPCR inhibitor, 4-DAMP, and siRNA against the M(3)-subtype GPCR. Carbachol 0-9 mitogen-activated protein kinase 1 Homo sapiens 21-24 19763792-5 2010 Carbachol-stimulated ERK activation and cell growth was completely blocked by the Muscarinic M(3)-subtype GPCR inhibitor, 4-DAMP, and siRNA against the M(3)-subtype GPCR. Carbachol 0-9 C-X-C motif chemokine receptor 6 Homo sapiens 106-110 19763792-5 2010 Carbachol-stimulated ERK activation and cell growth was completely blocked by the Muscarinic M(3)-subtype GPCR inhibitor, 4-DAMP, and siRNA against the M(3)-subtype GPCR. Carbachol 0-9 C-X-C motif chemokine receptor 6 Homo sapiens 165-169 19763792-6 2010 Interestingly, blockade of CaM KK with the selective inhibitor STO-609 prevented carbachol activation CaM KI, ERK, Elk-1, and cell growth. Carbachol 81-90 calcium/calmodulin dependent protein kinase kinase 2 Homo sapiens 27-33 19763792-6 2010 Interestingly, blockade of CaM KK with the selective inhibitor STO-609 prevented carbachol activation CaM KI, ERK, Elk-1, and cell growth. Carbachol 81-90 ETS transcription factor ELK1 Homo sapiens 115-120 19763792-7 2010 Consistent with these observations, knockdown of CaM KKalpha and CaM KIgamma with shRNA-containing plasmids blocked ERK activation by carbachol. Carbachol 134-143 mitogen-activated protein kinase 1 Homo sapiens 116-119 19763792-8 2010 In addition, Elk-1 phosphorylation and luciferase activity in response to carbachol treatment was also dependent upon CaM kinases and was inhibited by U0126, STO-609, and siRNA knockdown of CaM kinases and ERK2. Carbachol 74-83 ETS transcription factor ELK1 Homo sapiens 13-18 19763792-8 2010 In addition, Elk-1 phosphorylation and luciferase activity in response to carbachol treatment was also dependent upon CaM kinases and was inhibited by U0126, STO-609, and siRNA knockdown of CaM kinases and ERK2. Carbachol 74-83 mitogen-activated protein kinase 1 Homo sapiens 206-210 19763792-9 2010 Finally, blockade of either CaM KK (with STO-609) or ERK (with U0126) activities resulted in the inhibition of carbachol- and estrogen-mediated cyclin D1 expression and MCF-7 cell growth. Carbachol 111-120 calcium/calmodulin dependent protein kinase kinase 2 Homo sapiens 28-34 19763792-9 2010 Finally, blockade of either CaM KK (with STO-609) or ERK (with U0126) activities resulted in the inhibition of carbachol- and estrogen-mediated cyclin D1 expression and MCF-7 cell growth. Carbachol 111-120 mitogen-activated protein kinase 1 Homo sapiens 53-56 19763792-9 2010 Finally, blockade of either CaM KK (with STO-609) or ERK (with U0126) activities resulted in the inhibition of carbachol- and estrogen-mediated cyclin D1 expression and MCF-7 cell growth. Carbachol 111-120 cyclin D1 Homo sapiens 144-153 19864322-3 2010 We previously showed that activation of protein kinase C (PKC) by carbachol and phorbol 12-myristate 13-acetate (PMA) decreases NKCC1 surface expression in T84 cells. Carbachol 66-75 solute carrier family 12 member 2 Homo sapiens 128-133 19763792-10 2010 Taken together, our results suggest that carbachol treatment of MCF-7 cells activates CaM KI, ERK, the transcription factor Elk-1, cyclin D1, and cell growth through CaM KK. Carbachol 41-50 mitogen-activated protein kinase 1 Homo sapiens 94-97 19763792-10 2010 Taken together, our results suggest that carbachol treatment of MCF-7 cells activates CaM KI, ERK, the transcription factor Elk-1, cyclin D1, and cell growth through CaM KK. Carbachol 41-50 ETS transcription factor ELK1 Homo sapiens 124-129 19763792-10 2010 Taken together, our results suggest that carbachol treatment of MCF-7 cells activates CaM KI, ERK, the transcription factor Elk-1, cyclin D1, and cell growth through CaM KK. Carbachol 41-50 cyclin D1 Homo sapiens 131-140 19763792-10 2010 Taken together, our results suggest that carbachol treatment of MCF-7 cells activates CaM KI, ERK, the transcription factor Elk-1, cyclin D1, and cell growth through CaM KK. Carbachol 41-50 calcium/calmodulin dependent protein kinase kinase 2 Homo sapiens 166-172 20021253-9 2010 The increased expression and secretion of TGF-beta(2) caused by carbachol were suppressed by atropine (in the range of 10 nM-100 microM) when compared to treatment with carbachol alone (p < 0.001). Carbachol 169-178 transforming growth factor beta 2 Homo sapiens 42-50 20157258-6 2010 In CPu, the Abeta ability to impair the DA release evoked by the cholinergic agonist carbachol, observed in NAc, was confirmed only in vitro. Carbachol 85-94 amyloid beta precursor protein Rattus norvegicus 12-17 19875701-5 2010 Western blot analysis revealed that a prototypical Ca2+-dependent secretagogue, carbachol (CCh; 100 microM), induced phosphorylation of both the 46-kDa and 54-kDa isoforms of JNK. Carbachol 80-89 mitogen-activated protein kinase 8 Homo sapiens 175-178 19875701-5 2010 Western blot analysis revealed that a prototypical Ca2+-dependent secretagogue, carbachol (CCh; 100 microM), induced phosphorylation of both the 46-kDa and 54-kDa isoforms of JNK. Carbachol 91-94 mitogen-activated protein kinase 8 Homo sapiens 175-178 19875701-7 2010 CCh-induced JNK phosphorylation was attenuated by the EGFR inhibitor, tyrphostin-AG1478 (1 microM). Carbachol 0-3 mitogen-activated protein kinase 8 Homo sapiens 12-15 19875701-7 2010 CCh-induced JNK phosphorylation was attenuated by the EGFR inhibitor, tyrphostin-AG1478 (1 microM). Carbachol 0-3 epidermal growth factor receptor Homo sapiens 54-58 19875701-8 2010 Pretreatment of voltage-clamped T(84) cells with SP600125 (2 microM), a specific JNK inhibitor, potentiated secretory responses to both CCh and TG but not to FSK. Carbachol 136-139 mitogen-activated protein kinase 8 Homo sapiens 81-84 19373133-6 2010 The results indicated that PKC-alpha and PKC-epsilon agonists thymelea toxin and carbachol seemed to increase the calcium sensitivity and MLC20 phosphorylation of superior mesenteric artery after hemorrhagic shock and antagonize the increase of MLCP activity in VSMC after hypoxia. Carbachol 81-90 protein kinase C, alpha Rattus norvegicus 27-36 19921993-7 2010 An over-activated fetal renin-angiotensin-system (RAS) is associated with changes in vascular pressure following intravenous administration of carbachol, indicating that the cholinergic stimulation-mediated hormonal mechanism in the fetus might play a critical role in the regulation of cardiovascular homeostasis. Carbachol 143-152 renin Ovis aries 24-29 19373133-6 2010 The results indicated that PKC-alpha and PKC-epsilon agonists thymelea toxin and carbachol seemed to increase the calcium sensitivity and MLC20 phosphorylation of superior mesenteric artery after hemorrhagic shock and antagonize the increase of MLCP activity in VSMC after hypoxia. Carbachol 81-90 myosin light chain 12B Rattus norvegicus 138-143 19779011-5 2009 Accordingly, in isolated colonic crypts pretreated with forskolin and carbachol for 10 min, respectively, and subjected to immunohistochemistry, the NBCe1 signal showed a markedly stronger colocalization with the E-cadherin signal, which was used as a membrane marker, compared with the untreated control. Carbachol 70-79 cadherin 1 Mus musculus 213-223 20021253-6 2010 RESULTS: Carbachol induced a time-dependent increase in the levels of TGF-beta(2) mRNA and protein in the cytoplasm (p < 0.001). Carbachol 9-18 transforming growth factor beta 2 Homo sapiens 70-78 20021253-9 2010 The increased expression and secretion of TGF-beta(2) caused by carbachol were suppressed by atropine (in the range of 10 nM-100 microM) when compared to treatment with carbachol alone (p < 0.001). Carbachol 64-73 transforming growth factor beta 2 Homo sapiens 42-50 19749081-0 2009 Involvement of cyclooxygenase-2 in carbachol-induced positive inotropic response in mouse isolated left atrium. Carbachol 35-44 prostaglandin-endoperoxide synthase 2 Mus musculus 15-31 19460789-4 2009 The muscarinic receptor agonists carbachol and methacholine both induced modest effects on basal interleukin (IL)-8 and -6 secretion, whereas the secretion of RANTES, eotaxin, vascular endothelial growth factor-A and monocyte chemoattractant protein-1 was not affected. Carbachol 33-42 vascular endothelial growth factor A Homo sapiens 176-212 19460789-4 2009 The muscarinic receptor agonists carbachol and methacholine both induced modest effects on basal interleukin (IL)-8 and -6 secretion, whereas the secretion of RANTES, eotaxin, vascular endothelial growth factor-A and monocyte chemoattractant protein-1 was not affected. Carbachol 33-42 C-C motif chemokine ligand 2 Homo sapiens 217-251 20046026-6 2009 In organ culture, treatment of ileal tissue with 17beta-estradiol greatly suppressed the carbachol-induced increase in phosphorylation at Thr38 in CPI-17 without altering total CPI-17 protein expression. Carbachol 89-98 protein phosphatase 1, regulatory (inhibitor) subunit 14A Rattus norvegicus 147-153 19749081-3 2009 In this study, the involvement of cyclooxygenase (COX)-2 in carbachol (CCh)-induced inotropic response was investigated in mouse isolated left atrium. Carbachol 60-69 cytochrome c oxidase II, mitochondrial Mus musculus 34-56 19749081-3 2009 In this study, the involvement of cyclooxygenase (COX)-2 in carbachol (CCh)-induced inotropic response was investigated in mouse isolated left atrium. Carbachol 71-74 cytochrome c oxidase II, mitochondrial Mus musculus 34-56 19542247-10 2009 In separate experiments, incubation of PCLS with IL-13 or TNFalpha (100 ng/ml) increased airway sensitivity to carbachol. Carbachol 111-120 interleukin 13 Homo sapiens 49-54 19393327-6 2009 BNP induced a weak relaxant activity on carbachol-contracted bronchi in nonsensitized (relaxation: 4.23+/-0.51%) and passively sensitized bronchi (relaxation: 11.31+/-2.22%). Carbachol 40-49 natriuretic peptide B Homo sapiens 0-3 19673741-4 2009 The cholinergic agonist carbachol has been shown to induce axonal outgrowth through intracellular calcium mobilization, protein kinase C (PKC) activation, and ERK1/2 phosphorylation. Carbachol 24-33 protein kinase C, gamma Rattus norvegicus 120-136 19673741-4 2009 The cholinergic agonist carbachol has been shown to induce axonal outgrowth through intracellular calcium mobilization, protein kinase C (PKC) activation, and ERK1/2 phosphorylation. Carbachol 24-33 protein kinase C, gamma Rattus norvegicus 138-141 19673741-4 2009 The cholinergic agonist carbachol has been shown to induce axonal outgrowth through intracellular calcium mobilization, protein kinase C (PKC) activation, and ERK1/2 phosphorylation. Carbachol 24-33 mitogen activated protein kinase 3 Rattus norvegicus 159-165 19673741-13 2009 CONCLUSIONS: Ethanol inhibited carbachol-induced neurite outgrowth by inhibiting PKC and ERK1/2 activation. Carbachol 31-40 protein kinase C, gamma Rattus norvegicus 81-84 19673741-13 2009 CONCLUSIONS: Ethanol inhibited carbachol-induced neurite outgrowth by inhibiting PKC and ERK1/2 activation. Carbachol 31-40 mitogen activated protein kinase 3 Rattus norvegicus 89-95 19801823-5 2009 Carbacol and nerve growth factor (NGF), which are ERK1/2 activators, protected the neurons after CPF withdrawal, while atropine and PD98059, which are ERK1/2 inhibitors, exacerbated the cytotoxicity, indicating the involvement of inhibition of ERK1/2 phosphorylation in CPF-induced delayed cytotoxicity. Carbachol 0-8 mitogen activated protein kinase 3 Rattus norvegicus 50-56 19615971-0 2009 Carbachol induces p70S6K1 activation through an ERK-dependent but Akt-independent pathway in human colonic epithelial cells. Carbachol 0-9 EPH receptor B2 Homo sapiens 48-51 19615971-0 2009 Carbachol induces p70S6K1 activation through an ERK-dependent but Akt-independent pathway in human colonic epithelial cells. Carbachol 0-9 AKT serine/threonine kinase 1 Homo sapiens 66-69 19615971-1 2009 Stimulation of human colonic epithelial T84 cells with the muscarinic receptor agonist carbachol, a stable analog of acetylcholine, induced Akt, p70S6K1 and ERK activation. Carbachol 87-96 AKT serine/threonine kinase 1 Homo sapiens 140-143 19615971-1 2009 Stimulation of human colonic epithelial T84 cells with the muscarinic receptor agonist carbachol, a stable analog of acetylcholine, induced Akt, p70S6K1 and ERK activation. Carbachol 87-96 EPH receptor B2 Homo sapiens 157-160 19615971-2 2009 Treatment of T84 cells with the selective inhibitor of EGF receptor (EGFR) tyrosine kinase AG1478 abrogated Akt phosphorylation on Ser(473) induced by either carbachol or EGF, indicating that carbachol-induced Akt activation is mediated through EGFR transactivation. Carbachol 158-167 epidermal growth factor receptor Homo sapiens 55-67 19615971-2 2009 Treatment of T84 cells with the selective inhibitor of EGF receptor (EGFR) tyrosine kinase AG1478 abrogated Akt phosphorylation on Ser(473) induced by either carbachol or EGF, indicating that carbachol-induced Akt activation is mediated through EGFR transactivation. Carbachol 158-167 epidermal growth factor receptor Homo sapiens 69-73 19615971-2 2009 Treatment of T84 cells with the selective inhibitor of EGF receptor (EGFR) tyrosine kinase AG1478 abrogated Akt phosphorylation on Ser(473) induced by either carbachol or EGF, indicating that carbachol-induced Akt activation is mediated through EGFR transactivation. Carbachol 158-167 AKT serine/threonine kinase 1 Homo sapiens 108-111 19615971-2 2009 Treatment of T84 cells with the selective inhibitor of EGF receptor (EGFR) tyrosine kinase AG1478 abrogated Akt phosphorylation on Ser(473) induced by either carbachol or EGF, indicating that carbachol-induced Akt activation is mediated through EGFR transactivation. Carbachol 192-201 epidermal growth factor receptor Homo sapiens 55-67 19615971-2 2009 Treatment of T84 cells with the selective inhibitor of EGF receptor (EGFR) tyrosine kinase AG1478 abrogated Akt phosphorylation on Ser(473) induced by either carbachol or EGF, indicating that carbachol-induced Akt activation is mediated through EGFR transactivation. Carbachol 192-201 epidermal growth factor receptor Homo sapiens 69-73 19615971-2 2009 Treatment of T84 cells with the selective inhibitor of EGF receptor (EGFR) tyrosine kinase AG1478 abrogated Akt phosphorylation on Ser(473) induced by either carbachol or EGF, indicating that carbachol-induced Akt activation is mediated through EGFR transactivation. Carbachol 192-201 AKT serine/threonine kinase 1 Homo sapiens 108-111 19659456-1 2009 We tested the hypothesis that the de-endothelialized artery rings from the left anterior descending (LAD) coronary artery and its left ventricular branch (LVB) differ in their contractile responses to Na(+)-Ca(2+)-exchanger (NCX) mediated Ca(2+)-entry, muscarinic receptor activation with carbachol, and sarco/endoplasmic reticulum Ca(2+) pump (SERCA) inhibition with thapsigargin. Carbachol 289-298 solute carrier family 8 member A1 Homo sapiens 201-223 19751772-5 2009 Suppression of SPFH1 and SPFH2 expression by RNA interference markedly inhibited carbachol-induced IP(3) receptor polyubiquitination and degradation, but did not affect carbachol-induced calcium mobilization or IkappaBalpha processing, indicating that the SPFH1/2 complex is a key player in IP(3) receptor ERAD, acting at a step after IP(3) receptor activation, but prior to IP(3) receptor polyubiquitination. Carbachol 81-90 ER lipid raft associated 1 Homo sapiens 15-20 19751772-5 2009 Suppression of SPFH1 and SPFH2 expression by RNA interference markedly inhibited carbachol-induced IP(3) receptor polyubiquitination and degradation, but did not affect carbachol-induced calcium mobilization or IkappaBalpha processing, indicating that the SPFH1/2 complex is a key player in IP(3) receptor ERAD, acting at a step after IP(3) receptor activation, but prior to IP(3) receptor polyubiquitination. Carbachol 81-90 ER lipid raft associated 2 Homo sapiens 25-30 19751772-5 2009 Suppression of SPFH1 and SPFH2 expression by RNA interference markedly inhibited carbachol-induced IP(3) receptor polyubiquitination and degradation, but did not affect carbachol-induced calcium mobilization or IkappaBalpha processing, indicating that the SPFH1/2 complex is a key player in IP(3) receptor ERAD, acting at a step after IP(3) receptor activation, but prior to IP(3) receptor polyubiquitination. Carbachol 81-90 inositol 1,4,5-trisphosphate receptor type 3 Homo sapiens 99-113 19751772-5 2009 Suppression of SPFH1 and SPFH2 expression by RNA interference markedly inhibited carbachol-induced IP(3) receptor polyubiquitination and degradation, but did not affect carbachol-induced calcium mobilization or IkappaBalpha processing, indicating that the SPFH1/2 complex is a key player in IP(3) receptor ERAD, acting at a step after IP(3) receptor activation, but prior to IP(3) receptor polyubiquitination. Carbachol 81-90 ER lipid raft associated 1 Homo sapiens 256-263 19751772-5 2009 Suppression of SPFH1 and SPFH2 expression by RNA interference markedly inhibited carbachol-induced IP(3) receptor polyubiquitination and degradation, but did not affect carbachol-induced calcium mobilization or IkappaBalpha processing, indicating that the SPFH1/2 complex is a key player in IP(3) receptor ERAD, acting at a step after IP(3) receptor activation, but prior to IP(3) receptor polyubiquitination. Carbachol 81-90 inositol 1,4,5-trisphosphate receptor type 3 Homo sapiens 291-305 19751772-5 2009 Suppression of SPFH1 and SPFH2 expression by RNA interference markedly inhibited carbachol-induced IP(3) receptor polyubiquitination and degradation, but did not affect carbachol-induced calcium mobilization or IkappaBalpha processing, indicating that the SPFH1/2 complex is a key player in IP(3) receptor ERAD, acting at a step after IP(3) receptor activation, but prior to IP(3) receptor polyubiquitination. Carbachol 81-90 inositol 1,4,5-trisphosphate receptor type 3 Homo sapiens 291-305 19751772-5 2009 Suppression of SPFH1 and SPFH2 expression by RNA interference markedly inhibited carbachol-induced IP(3) receptor polyubiquitination and degradation, but did not affect carbachol-induced calcium mobilization or IkappaBalpha processing, indicating that the SPFH1/2 complex is a key player in IP(3) receptor ERAD, acting at a step after IP(3) receptor activation, but prior to IP(3) receptor polyubiquitination. Carbachol 81-90 inositol 1,4,5-trisphosphate receptor type 3 Homo sapiens 291-305 19626679-1 2009 Regulations of intracellular protein kinase C (PKC) on carbachol (CCh)-induced intracellular calcium ([Ca(2+)]i) responses were investigated in different stages of melanoma cells. Carbachol 55-64 protein kinase C alpha Homo sapiens 47-50 19626679-3 2009 Pretreatment of phorbol 12, 13-dibutyrate (PDBu, 2 microM), an activator of intracellular PKC, significantly suppressed CCh-induced peak reactions in WM793B, SK-MEL-5, and A2058 cells. Carbachol 120-123 protein kinase C alpha Homo sapiens 90-93 19626679-5 2009 Short interfering RNA (siRNA) targeting to PKCalpha in WM793B cells enhanced CCh-induced peak calcium reactions. Carbachol 77-80 protein kinase C alpha Homo sapiens 43-51 19626679-7 2009 Moreover, intracellular PKCalpha activated by exogenous agonist and expressed through endogenous gene transcription negatively regulated CCh-induced calcium responses. Carbachol 137-140 protein kinase C alpha Homo sapiens 24-32 19626679-8 2009 The functional analysis on the relationship between CCh-induced calcium response and endogenous PKCalpha expression might be helpful to predict the development of melanoma. Carbachol 52-55 protein kinase C alpha Homo sapiens 96-104 19680632-6 2009 In contrast, in HEK(hUT) cells, primary stimulation with carbachol (250 microM) reduced a secondary challenge to U-II (1 microM) by 84% and primary challenge with U-II reduced a secondary challenge to carbachol by 76%. Carbachol 57-66 urotensin 2 Homo sapiens 113-117 19680632-6 2009 In contrast, in HEK(hUT) cells, primary stimulation with carbachol (250 microM) reduced a secondary challenge to U-II (1 microM) by 84% and primary challenge with U-II reduced a secondary challenge to carbachol by 76%. Carbachol 201-210 urotensin 2 Homo sapiens 163-167 19628652-8 2009 In Western blot analysis, 8-Br-cGMP reduced the signal for phosphorylated MYPT-1 in carbachol-stimulated jejunum but not in colon. Carbachol 84-93 protein phosphatase 1, regulatory subunit 12A Mus musculus 74-80 19549525-6 2009 In TRPC4-deficient ileal myocytes the carbachol-induced membrane depolarizations are diminished greatly and the atropine-sensitive contraction elicited by acetylcholine release from excitatory motor neurons is reduced greatly. Carbachol 38-47 transient receptor potential cation channel, subfamily C, member 4 Mus musculus 3-8 20149029-5 2009 RESULTS: All curves were displaced to the right by hCG in a concentration-dependent manner with significant inhibition of contractions induced by carbachol (P < 0.001) and KCl (P = 0.016) but not those induced by alpha,beta-methylene ATP (P = 0.4). Carbachol 146-155 chorionic gonadotropin subunit beta 5 Homo sapiens 51-54 20149029-8 2009 CONCLUSIONS: hCG significantly inhibited in vitro detrusor contractions induced by depolarization (KCl) and cholinergic (carbachol) but not purinergic (alpha,beta-methylene ATP) stimulation in a dose-dependent manner in female rats. Carbachol 121-130 chorionic gonadotropin subunit beta 5 Homo sapiens 13-16 19615971-4 2009 In contrast, treatment with the selective MEK inhibitor U0126 (but not with the inactive analog U0124) inhibited carbachol-induced p70S6K1 activation, indicating that the MEK/ERK/RSK pathway plays a critical role in p70S6K1 activation in GPCR-stimulated T84 cells. Carbachol 113-122 mitogen-activated protein kinase kinase 7 Homo sapiens 42-45 19615971-4 2009 In contrast, treatment with the selective MEK inhibitor U0126 (but not with the inactive analog U0124) inhibited carbachol-induced p70S6K1 activation, indicating that the MEK/ERK/RSK pathway plays a critical role in p70S6K1 activation in GPCR-stimulated T84 cells. Carbachol 113-122 mitogen-activated protein kinase kinase 7 Homo sapiens 171-174 19615971-4 2009 In contrast, treatment with the selective MEK inhibitor U0126 (but not with the inactive analog U0124) inhibited carbachol-induced p70S6K1 activation, indicating that the MEK/ERK/RSK pathway plays a critical role in p70S6K1 activation in GPCR-stimulated T84 cells. Carbachol 113-122 EPH receptor B2 Homo sapiens 175-178 19615971-4 2009 In contrast, treatment with the selective MEK inhibitor U0126 (but not with the inactive analog U0124) inhibited carbachol-induced p70S6K1 activation, indicating that the MEK/ERK/RSK pathway plays a critical role in p70S6K1 activation in GPCR-stimulated T84 cells. Carbachol 113-122 ribosomal protein S6 kinase A2 Homo sapiens 179-182 19542247-10 2009 In separate experiments, incubation of PCLS with IL-13 or TNFalpha (100 ng/ml) increased airway sensitivity to carbachol. Carbachol 111-120 tumor necrosis factor Homo sapiens 58-66 19535329-6 2009 Similarly, in Caco-2BBe cells, NHERF3 and NHE3 colocalized in the BB under basal conditions but after elevation of [Ca(2+)](i) by carbachol, this overlap was abolished. Carbachol 130-139 PDZ domain containing 1 Homo sapiens 31-37 19497958-2 2009 Addition of PYY or neuropeptide-Y (NPY) to the bathing solution of mucosae in Ussing chambers suppressed the short-circuit current (Isc) corresponding to electrogenic Cl- secretion, whether stimulated by epinephrine (epi), prostaglandin-E2 (PGE2), or carbachol (CCh). Carbachol 251-260 peptide YY Cavia porcellus 12-15 19497958-2 2009 Addition of PYY or neuropeptide-Y (NPY) to the bathing solution of mucosae in Ussing chambers suppressed the short-circuit current (Isc) corresponding to electrogenic Cl- secretion, whether stimulated by epinephrine (epi), prostaglandin-E2 (PGE2), or carbachol (CCh). Carbachol 251-260 pro-neuropeptide Y Cavia porcellus 19-33 19497958-2 2009 Addition of PYY or neuropeptide-Y (NPY) to the bathing solution of mucosae in Ussing chambers suppressed the short-circuit current (Isc) corresponding to electrogenic Cl- secretion, whether stimulated by epinephrine (epi), prostaglandin-E2 (PGE2), or carbachol (CCh). Carbachol 251-260 pro-neuropeptide Y Cavia porcellus 35-38 19497958-2 2009 Addition of PYY or neuropeptide-Y (NPY) to the bathing solution of mucosae in Ussing chambers suppressed the short-circuit current (Isc) corresponding to electrogenic Cl- secretion, whether stimulated by epinephrine (epi), prostaglandin-E2 (PGE2), or carbachol (CCh). Carbachol 262-265 peptide YY Cavia porcellus 12-15 19497958-2 2009 Addition of PYY or neuropeptide-Y (NPY) to the bathing solution of mucosae in Ussing chambers suppressed the short-circuit current (Isc) corresponding to electrogenic Cl- secretion, whether stimulated by epinephrine (epi), prostaglandin-E2 (PGE2), or carbachol (CCh). Carbachol 262-265 pro-neuropeptide Y Cavia porcellus 19-33 19497958-2 2009 Addition of PYY or neuropeptide-Y (NPY) to the bathing solution of mucosae in Ussing chambers suppressed the short-circuit current (Isc) corresponding to electrogenic Cl- secretion, whether stimulated by epinephrine (epi), prostaglandin-E2 (PGE2), or carbachol (CCh). Carbachol 262-265 pro-neuropeptide Y Cavia porcellus 35-38 19535329-8 2009 Also, carbachol-mediated inhibition of NHE3 activity was abolished in Caco-2BBe cells in which NHERF3 protein expression was significantly reduced. Carbachol 6-15 solute carrier family 9 member A3 Homo sapiens 39-43 19535329-8 2009 Also, carbachol-mediated inhibition of NHE3 activity was abolished in Caco-2BBe cells in which NHERF3 protein expression was significantly reduced. Carbachol 6-15 PDZ domain containing 1 Homo sapiens 95-101 19656467-5 2009 Carbachol (CCH; 300 microM) was able to evoke glutamate release more efficiently from PC12-NCS-1 (15.3+/-1.0nmol/mg of protein) than wild type cells (PC12-wt; 8.3+/-0.9nmol/mg of protein). Carbachol 0-9 neuronal calcium sensor 1 Rattus norvegicus 86-96 19656467-5 2009 Carbachol (CCH; 300 microM) was able to evoke glutamate release more efficiently from PC12-NCS-1 (15.3+/-1.0nmol/mg of protein) than wild type cells (PC12-wt; 8.3+/-0.9nmol/mg of protein). Carbachol 11-14 neuronal calcium sensor 1 Rattus norvegicus 86-96 19656467-8 2009 and [Ca2+]i (766.4+/-40.0 and 687.8+/-37.1nmol/L) was observed after CCH stimulus of PC12-NCS-1 compared with PC12-wt. Carbachol 69-72 neuronal calcium sensor 1 Rattus norvegicus 85-95 19656467-13 2009 Together, our data show that overexpression of NCS-1 in PC12 cells induces an enhancement of intracellular second messenger and transmitter release dependent on CCH response, suggesting that muscarinic signaling is "up-regulated" in this cell model. Carbachol 161-164 neuronal calcium sensor 1 Rattus norvegicus 47-52 19457892-4 2009 Confocal fluorescence microscopy indicated that cp-Galpha(i)1/2 and/or cp-Galpha(i)3 peptides moderated carbachol attenuation of cellular Ca(2+) transients in isolated atrial myocytes. Carbachol 104-113 protein phosphatase 1 regulatory inhibitor subunit 1A Canis lupus familiaris 48-63 19535329-6 2009 Similarly, in Caco-2BBe cells, NHERF3 and NHE3 colocalized in the BB under basal conditions but after elevation of [Ca(2+)](i) by carbachol, this overlap was abolished. Carbachol 130-139 solute carrier family 9 member A3 Homo sapiens 42-46 19005736-6 2009 The increase in IP accumulation induced by CCh after TNF-alpha immersion was reduced in the BSMCs by LLLT. Carbachol 43-46 tumor necrosis factor Rattus norvegicus 53-62 19558456-2 2009 In hippocampal primary and cerebellar granule neuron cultures expressing endogenous M1 receptor, carbachol increased the levels of a prototypical phase II antioxidant enzyme, heme oxygenase-1. Carbachol 97-106 heme oxygenase 1 Rattus norvegicus 175-191 19352616-10 2009 CONCLUSION: Despite the fact that inhibitors of PDE1, PDE4, and PDE5 exerted only a weak relaxant response on detrusor strips precontracted by carbachol, our findings indicate that both the cAMP and cGMP pathways might be involved in the relaxation mechanism of human detrusor smooth muscle. Carbachol 143-152 phosphodiesterase 4A Homo sapiens 54-58 19578554-11 2009 Carbachol also activated p42/44 mitogen-activated protein kinase (MAPK) and Ras in a time-dependent manner. Carbachol 0-9 mitogen-activated protein kinase 1 Mus musculus 66-70 19352616-10 2009 CONCLUSION: Despite the fact that inhibitors of PDE1, PDE4, and PDE5 exerted only a weak relaxant response on detrusor strips precontracted by carbachol, our findings indicate that both the cAMP and cGMP pathways might be involved in the relaxation mechanism of human detrusor smooth muscle. Carbachol 143-152 phosphodiesterase 5A Homo sapiens 64-68 19190235-7 2009 In addition, down-regulation of ERK1/2 with small interfering RNA abolished the neuritogenic effect of carbachol. Carbachol 103-112 mitogen activated protein kinase 3 Rattus norvegicus 32-38 19356605-6 2009 In another set of experiments, the specific NK1 receptor antagonist L-703,606 (5 microg) was microinjected in the PAG following LH stimulation with carbachol abolished LH-induced antinociception as well. Carbachol 148-157 tachykinin receptor 1 Rattus norvegicus 44-56 19200344-2 2009 By activating M(3) muscarinic receptors, the cholinergic agonist carbachol inhibits DomA-induced apoptosis, and the anti-apoptotic action of carbachol is more pronounced in CGNs from Gclm (+/+) mice. Carbachol 141-150 glutamate-cysteine ligase, modifier subunit Mus musculus 183-187 19443836-4 2009 In HEK293 cells expressing TRPC6, application of mechanical stimuli (hypotonicity, shear, 2,4,6-trinitrophenol) caused, albeit not effective by themselves, a prominent potentiation of cationic currents (I(TRPC6)) induced by a muscarinic receptor agonist carbachol. Carbachol 254-263 transient receptor potential cation channel subfamily C member 6 Homo sapiens 27-32 19443836-7 2009 Single TRPC6 channel activity evoked by carbachol was also enhanced by a negative pressure added in the patch pipette. Carbachol 40-49 transient receptor potential cation channel subfamily C member 6 Homo sapiens 7-12 19443836-8 2009 Mechanical potentiation of carbachol- or OAG-induced I(TRPC6) was abolished by small interfering RNA knockdown of cytosolic phospholipase A(2) or pharmacological inhibition of omega-hydroxylation of arachidonic acid into 20-HETE (20-hydroxyeicosatetraenoic acid). Carbachol 27-36 transient receptor potential cation channel subfamily C member 6 Homo sapiens 55-60 19450258-9 2009 Decreased sensitivity to carbachol was associated with increased expression of IL-4 and IL-6 mRNA in jejunum. Carbachol 25-34 interleukin 4 Mus musculus 79-83 19450258-9 2009 Decreased sensitivity to carbachol was associated with increased expression of IL-4 and IL-6 mRNA in jejunum. Carbachol 25-34 interleukin 6 Mus musculus 88-92 19332491-5 2009 Specifically, Ca(2+) entry seen after carbachol treatment in cells transiently expressing TRPC1, TRPC3, TRPC5 or TRPC6 was not enhanced by the co-expression of STIM1. Carbachol 38-47 transient receptor potential cation channel subfamily C member 1 Homo sapiens 90-95 19332491-5 2009 Specifically, Ca(2+) entry seen after carbachol treatment in cells transiently expressing TRPC1, TRPC3, TRPC5 or TRPC6 was not enhanced by the co-expression of STIM1. Carbachol 38-47 transient receptor potential cation channel subfamily C member 3 Homo sapiens 97-102 19332491-5 2009 Specifically, Ca(2+) entry seen after carbachol treatment in cells transiently expressing TRPC1, TRPC3, TRPC5 or TRPC6 was not enhanced by the co-expression of STIM1. Carbachol 38-47 transient receptor potential cation channel subfamily C member 5 Homo sapiens 104-109 19332491-5 2009 Specifically, Ca(2+) entry seen after carbachol treatment in cells transiently expressing TRPC1, TRPC3, TRPC5 or TRPC6 was not enhanced by the co-expression of STIM1. Carbachol 38-47 transient receptor potential cation channel subfamily C member 6 Homo sapiens 113-118 19190235-8 2009 These data suggest an involvement of Ca(2+), PKC, and ERK1/2 in carbachol-induced axonal growth. Carbachol 64-73 protein kinase C, gamma Rattus norvegicus 45-48 19190235-8 2009 These data suggest an involvement of Ca(2+), PKC, and ERK1/2 in carbachol-induced axonal growth. Carbachol 64-73 mitogen activated protein kinase 3 Rattus norvegicus 54-60 19190235-9 2009 Carbachol indeed increased the release of Ca(2+) from intracellular stores and induced PKC and ERK1/2 activation. Carbachol 0-9 protein kinase C, gamma Rattus norvegicus 87-90 19190235-9 2009 Carbachol indeed increased the release of Ca(2+) from intracellular stores and induced PKC and ERK1/2 activation. Carbachol 0-9 mitogen activated protein kinase 3 Rattus norvegicus 95-101 19190235-10 2009 Additional experiments showed that PKC, but not Ca(2+), is involved in carbachol-induced ERK1/2 activation. Carbachol 71-80 protein kinase C, gamma Rattus norvegicus 35-38 19190235-10 2009 Additional experiments showed that PKC, but not Ca(2+), is involved in carbachol-induced ERK1/2 activation. Carbachol 71-80 mitogen activated protein kinase 3 Rattus norvegicus 89-95 19190174-8 2009 The contributions of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) to CCh-induced contractions were significantly increased during colitis. Carbachol 116-119 mitogen-activated protein kinase 1 Mus musculus 21-58 19190174-8 2009 The contributions of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) to CCh-induced contractions were significantly increased during colitis. Carbachol 116-119 mitogen-activated protein kinase 1 Mus musculus 60-63 19190174-8 2009 The contributions of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) to CCh-induced contractions were significantly increased during colitis. Carbachol 116-119 mitogen-activated protein kinase 1 Homo sapiens 107-111 19269274-3 2009 Consequently, we performed a quantitative, regionally-specific analysis of the Fos immunoreactivity of neurons in the POA of the cat during NREM sleep and REM sleep induced by microinjections of carbachol into the nucleus pontis oralis (REMc), as well as during quiet and alert wakefulness. Carbachol 195-204 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 79-82 19166928-8 2009 However, GI-hormones/neurotransmitters [CCK, bombesin, carbachol] activating phospholipase C (PLC) inhibited basal and growth-factor-stimulated Akt activation. Carbachol 55-64 AKT serine/threonine kinase 1 Homo sapiens 144-147 19200344-5 2009 Carbachol activates extracellular signal-regulated kinases 1/2 (ERK1/2) MAPK and phospahtidylinositol-3 kinase (PI3K) in CGNs from both genotypes. Carbachol 0-9 mitogen-activated protein kinase 3 Mus musculus 20-62 19200344-5 2009 Carbachol activates extracellular signal-regulated kinases 1/2 (ERK1/2) MAPK and phospahtidylinositol-3 kinase (PI3K) in CGNs from both genotypes. Carbachol 0-9 mitogen-activated protein kinase 3 Mus musculus 64-70 19200344-6 2009 However, while the protective effect of carbachol is mediated by ERK1/2 MAPK in CGNs from both mouse genotypes, inhibitors of PI3K are only effective at antagonizing the action of carbachol in CGNs from Gclm (+/+) mice. Carbachol 40-49 mitogen-activated protein kinase 3 Mus musculus 65-71 19200344-7 2009 In CGNs from both Gclm (+/+) and (-/-) mice, carbachol induces a MAPK-dependent increase in the level of the anti-apoptotic protein Bcl-2. Carbachol 45-54 glutamate-cysteine ligase, modifier subunit Mus musculus 18-22 19200344-7 2009 In CGNs from both Gclm (+/+) and (-/-) mice, carbachol induces a MAPK-dependent increase in the level of the anti-apoptotic protein Bcl-2. Carbachol 45-54 B cell leukemia/lymphoma 2 Mus musculus 132-137 19200344-8 2009 In contrast, carbachol causes a PI3K-dependent increase in GCL activity and of GSH levels only in CGNs from Gclm (+/+) mice. Carbachol 13-22 glutamate-cysteine ligase, modifier subunit Mus musculus 108-112 19200344-4 2009 Carbachol inhibits DomA-induced activation of Jun N-terminal (JNK) and p38 kinases, increased translocation to mitochondria of the pro-apoptotic protein Bax, and activation of caspase-3. Carbachol 0-9 mitogen-activated protein kinase 8 Mus musculus 62-65 19200344-4 2009 Carbachol inhibits DomA-induced activation of Jun N-terminal (JNK) and p38 kinases, increased translocation to mitochondria of the pro-apoptotic protein Bax, and activation of caspase-3. Carbachol 0-9 mitogen-activated protein kinase 14 Mus musculus 71-74 19200344-4 2009 Carbachol inhibits DomA-induced activation of Jun N-terminal (JNK) and p38 kinases, increased translocation to mitochondria of the pro-apoptotic protein Bax, and activation of caspase-3. Carbachol 0-9 BCL2-associated X protein Mus musculus 153-156 19200344-4 2009 Carbachol inhibits DomA-induced activation of Jun N-terminal (JNK) and p38 kinases, increased translocation to mitochondria of the pro-apoptotic protein Bax, and activation of caspase-3. Carbachol 0-9 caspase 3 Mus musculus 176-185 19109404-9 2009 GLP-2 induced concentration-dependent relaxation in carbachol-precontracted fundic strips but not in antral strips. Carbachol 52-61 glucagon-like peptide 2 receptor Mus musculus 0-5 19052105-3 2009 DSM strips from SM-B KO mice generated more force in response to electrical field stimulation, KCl, carbachol, and phorbol 12,13-dibutyrate than that of age-matched wild-type mice. Carbachol 100-109 small nuclear ribonucleoprotein B Mus musculus 16-20 19052105-7 2009 Two-dimensional gel electrophoresis revealed an increased level of MLC20 phosphorylation in response to carbachol. Carbachol 104-113 myosin, light chain 12B, regulatory Mus musculus 67-72 19109944-11 2009 A phospholipase C (PLC) inhibitor U73122, a protein kinase C (PKC) inhibitor chelerythrine and a phospholipase A(2) (PLA(2)) inhibitor AACOCF(3) inhibited the [Ca(2+)](i) response to carbamylcholine or oxotremorine M, while these inhibitors did not block the effect of nicotine. Carbachol 183-198 phospholipase A2, group IB, pancreas Mus musculus 117-123 19056381-0 2009 Transduced viral IL-10 is exocytosed from lacrimal acinar secretory vesicles in a myosin-dependent manner in response to carbachol. Carbachol 121-130 interleukin-10 Oryctolagus cuniculus 17-22 19141314-5 2009 The cholinergic agonist carbachol also depressed GluR1-3 mRNA levels, suggesting that AMPAR down-regulation is a global response to extended periods of elevated neuronal activity. Carbachol 24-33 glutamate ionotropic receptor AMPA type subunit 1 Rattus norvegicus 49-54 19070673-3 2009 Carbachol (CCh) dose-dependently stimulated both mTOR and p70S6K phosphorylations and these responses were abolished by pertussis toxin pretreatment, indicating the involvement of the G(i)-coupled M(4) mAChR. Carbachol 0-9 mechanistic target of rapamycin kinase Rattus norvegicus 49-53 19159405-9 2009 Carbachol-evoked inotropic responses and increase in phosphorylated MLC-2 were attenuated by MLC kinase (ML-9) and Rho-kinase inhibition (Y-27632), and inotropic responses were abolished by Pertussis toxin pretreatment. Carbachol 0-9 myosin light chain 2 Rattus norvegicus 68-73 18984743-10 2009 No difference in the switch-off rate of glucose-stimulated insulin secretion was observed between genotypes, but the cholinergic agonist carbamylcholine enhanced glucose-induced insulin secretion to a lesser extent in Sst(-/-) islets compared with controls. Carbachol 137-152 somatostatin Mus musculus 218-221 19166483-8 2009 However, when these stores were depleted by block of the sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA) pump or calcium release was activated by carbachol, the absence of Cav-1 or caveolae had little or no effect. Carbachol 148-157 ATPase, Ca++ transporting, ubiquitous Mus musculus 57-98 19166483-8 2009 However, when these stores were depleted by block of the sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA) pump or calcium release was activated by carbachol, the absence of Cav-1 or caveolae had little or no effect. Carbachol 148-157 ATPase, Ca++ transporting, ubiquitous Mus musculus 100-105 19070673-3 2009 Carbachol (CCh) dose-dependently stimulated both mTOR and p70S6K phosphorylations and these responses were abolished by pertussis toxin pretreatment, indicating the involvement of the G(i)-coupled M(4) mAChR. Carbachol 0-9 ribosomal protein S6 kinase B1 Rattus norvegicus 58-64 19070673-3 2009 Carbachol (CCh) dose-dependently stimulated both mTOR and p70S6K phosphorylations and these responses were abolished by pertussis toxin pretreatment, indicating the involvement of the G(i)-coupled M(4) mAChR. Carbachol 11-14 mechanistic target of rapamycin kinase Rattus norvegicus 49-53 19070673-3 2009 Carbachol (CCh) dose-dependently stimulated both mTOR and p70S6K phosphorylations and these responses were abolished by pertussis toxin pretreatment, indicating the involvement of the G(i)-coupled M(4) mAChR. Carbachol 11-14 ribosomal protein S6 kinase B1 Rattus norvegicus 58-64 19070673-6 2009 Although inhibition of protein phosphatase 2A by okadaic acid augmented the transient effects of CCh on Akt/tuberin phosphorylations, it failed to significantly prolong these responses. Carbachol 97-100 AKT serine/threonine kinase 1 Rattus norvegicus 104-107 19070673-6 2009 Although inhibition of protein phosphatase 2A by okadaic acid augmented the transient effects of CCh on Akt/tuberin phosphorylations, it failed to significantly prolong these responses. Carbachol 97-100 TSC complex subunit 2 Rattus norvegicus 108-115 19070673-7 2009 The total protein level of PTEN (tumor suppressor gene phosphatase and tensin homologue deleted on chromosome ten) was attenuated upon NGF, but not CCh treatment. Carbachol 148-151 phosphatase and tensin homolog Rattus norvegicus 27-31 19340558-8 2009 In addition, sensitivity to carbachol was impaired in mice without CCK-2R. Carbachol 28-37 cholecystokinin B receptor Mus musculus 67-73 18651719-2 2009 Although Abeta itself did not increase Ca(2+), it exacerbated the effects of carbachol. Carbachol 77-86 amyloid beta precursor protein Homo sapiens 9-14 19340558-10 2009 In conclusion, CCK-2R is necessary to respond to carbachol as well as to produce the maximal acid secretion, while the role of CCK-1R in acid secretion is less important. Carbachol 49-58 cholecystokinin B receptor Mus musculus 15-21 19093744-9 2009 In conclusion, ET(B) receptor stimulation relaxes the carbachol precontracted iris sphincter muscle, an effect that is mediated by the ET(B2) receptor subtype, through NO and the release of prostaglandins. Carbachol 54-63 endothelin receptor type B Oryctolagus cuniculus 15-19 18830899-15 2009 CONCLUSIONS: Based on these findings it is concluded that curcumin prevented the reduction in carbachol-induced contraction in trinitrobenzenesulphonic acid -treated rats by modulating NF-kB signaling pathway. Carbachol 94-103 nuclear factor kappa B subunit 1 Rattus norvegicus 185-190 18929546-4 2008 This paper examined the effects of acute PKC inhibition on firing responses to carbachol, NMDA or AMPA using patch clamp recordings from brain slices. Carbachol 79-88 proline rich transmembrane protein 2 Homo sapiens 41-44 19267388-9 2009 Pharmacological studies revealed a modest, gender-specific reduction in sensitivity of isolated detrusor strips from UPII KO female mice to carbachol-induced contractions. Carbachol 140-149 uroplakin 2 Mus musculus 117-121 19011095-6 2008 However, muscle strips from SM2(-/-) bladder showed increased contraction to K(+) depolarization or in response to M3 receptor agonist Carbachol. Carbachol 135-144 myosin, heavy polypeptide 11, smooth muscle Mus musculus 28-31 20201386-4 2009 Menthol (0.1-1 mmol/l) per se virtually unaffected the basal tone, but inhibited in a dose-dependent manner KCl-, CCh- and Nor-evoked contractions of both parts of the vas deference by 30-50%. Carbachol 114-117 arginine vasopressin Rattus norvegicus 168-171 18854172-7 2008 Coexpression with muscarinic receptor M2 induced TRPC4 current activation by muscarinic stimulation with carbachol, which was inhibited by pertussis toxin. Carbachol 105-114 transient receptor potential cation channel subfamily C member 4 Homo sapiens 49-54 18989912-3 2008 The high nAChR activity of carbamoylcholine analogue 5d was found to reside in its R-enantiomer, a characteristic most likely true for all other compounds in the series. Carbachol 27-43 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 9-14 19006330-0 2008 Analysis of the reaction of carbachol with acetylcholinesterase using thioflavin T as a coupled fluorescence reporter. Carbachol 28-37 acetylcholinesterase (Cartwright blood group) Homo sapiens 43-63 19006330-5 2008 Here, we focus on the reaction of carbachol (carbamoylcholine) with AChE. Carbachol 34-43 acetylcholinesterase (Cartwright blood group) Homo sapiens 68-72 19006330-5 2008 Here, we focus on the reaction of carbachol (carbamoylcholine) with AChE. Carbachol 45-61 acetylcholinesterase (Cartwright blood group) Homo sapiens 68-72 18832081-11 2008 Maximal cardioinhibitory response to the M(2)-receptor agonist carbachol (0.5 mg/kg) compared with basal heart rate was attenuated in Galphai2(-/-) mice (0.08 +/- 0.04; n = 6) compared to control (0.27 +/- 0.04; n = 7 P < 0.05). Carbachol 63-72 guanine nucleotide binding protein (G protein), alpha inhibiting 2 Mus musculus 134-142 18832449-9 2008 Carbachol-stimulated phosphorylation of S(136) was inhibited by the CAMK2 inhibitor KN93 (IC(50) 38 microM) and by the calmodulin antagonist W7 (IC(50) 3.3 nM). Carbachol 0-9 calcium/calmodulin dependent protein kinase II beta Homo sapiens 68-73 18832449-9 2008 Carbachol-stimulated phosphorylation of S(136) was inhibited by the CAMK2 inhibitor KN93 (IC(50) 38 microM) and by the calmodulin antagonist W7 (IC(50) 3.3 nM). Carbachol 0-9 calmodulin 1 Homo sapiens 119-129 18845574-4 2008 Here, we demonstrate that protein kinase D1 (PKD1) is a key downstream target of PKCdelta and PKCepsilon in pancreatic acinar cells stimulated by two major secretagogues, CCK-8 and the cholinergic agonist carbachol (CCh), and that PKD1 is necessary for NF-kappaB activation induced by CCK-8 and CCh. Carbachol 205-214 protein kinase D1 Rattus norvegicus 26-43 18845574-4 2008 Here, we demonstrate that protein kinase D1 (PKD1) is a key downstream target of PKCdelta and PKCepsilon in pancreatic acinar cells stimulated by two major secretagogues, CCK-8 and the cholinergic agonist carbachol (CCh), and that PKD1 is necessary for NF-kappaB activation induced by CCK-8 and CCh. Carbachol 205-214 protein kinase D1 Rattus norvegicus 45-49 18845574-4 2008 Here, we demonstrate that protein kinase D1 (PKD1) is a key downstream target of PKCdelta and PKCepsilon in pancreatic acinar cells stimulated by two major secretagogues, CCK-8 and the cholinergic agonist carbachol (CCh), and that PKD1 is necessary for NF-kappaB activation induced by CCK-8 and CCh. Carbachol 216-219 protein kinase D1 Rattus norvegicus 26-43 18845574-4 2008 Here, we demonstrate that protein kinase D1 (PKD1) is a key downstream target of PKCdelta and PKCepsilon in pancreatic acinar cells stimulated by two major secretagogues, CCK-8 and the cholinergic agonist carbachol (CCh), and that PKD1 is necessary for NF-kappaB activation induced by CCK-8 and CCh. Carbachol 216-219 protein kinase D1 Rattus norvegicus 45-49 18845574-4 2008 Here, we demonstrate that protein kinase D1 (PKD1) is a key downstream target of PKCdelta and PKCepsilon in pancreatic acinar cells stimulated by two major secretagogues, CCK-8 and the cholinergic agonist carbachol (CCh), and that PKD1 is necessary for NF-kappaB activation induced by CCK-8 and CCh. Carbachol 295-298 protein kinase D1 Rattus norvegicus 26-43 18845574-4 2008 Here, we demonstrate that protein kinase D1 (PKD1) is a key downstream target of PKCdelta and PKCepsilon in pancreatic acinar cells stimulated by two major secretagogues, CCK-8 and the cholinergic agonist carbachol (CCh), and that PKD1 is necessary for NF-kappaB activation induced by CCK-8 and CCh. Carbachol 295-298 protein kinase D1 Rattus norvegicus 45-49 18845574-5 2008 Both CCK-8 and CCh dose dependently induced a rapid and striking activation of PKD1 in rat pancreatic acinar cells, as measured by in vitro kinase assay and by phosphorylation at PKD1 activation loop (Ser744/748) or autophosphorylation site (Ser916). Carbachol 15-18 protein kinase D1 Rattus norvegicus 79-83 18845574-5 2008 Both CCK-8 and CCh dose dependently induced a rapid and striking activation of PKD1 in rat pancreatic acinar cells, as measured by in vitro kinase assay and by phosphorylation at PKD1 activation loop (Ser744/748) or autophosphorylation site (Ser916). Carbachol 15-18 protein kinase D1 Rattus norvegicus 179-183 18845574-6 2008 The phosphorylation and activation of PKD1 correlated with NF-kappaB activity stimulated by CCK-8 or CCh, as measured by NF-kappaB DNA binding. Carbachol 101-104 protein kinase D1 Rattus norvegicus 38-42 18845574-6 2008 The phosphorylation and activation of PKD1 correlated with NF-kappaB activity stimulated by CCK-8 or CCh, as measured by NF-kappaB DNA binding. Carbachol 101-104 nuclear factor kappa B subunit 1 Homo sapiens 59-68 18845574-6 2008 The phosphorylation and activation of PKD1 correlated with NF-kappaB activity stimulated by CCK-8 or CCh, as measured by NF-kappaB DNA binding. Carbachol 101-104 nuclear factor kappa B subunit 1 Homo sapiens 121-130 18845574-8 2008 Conversely, overexpression of PKD1 resulted in augmentation of CCK-8- and CCh-stimulated NF-kappaB activation. Carbachol 74-77 protein kinase D1 Rattus norvegicus 30-34 18845574-8 2008 Conversely, overexpression of PKD1 resulted in augmentation of CCK-8- and CCh-stimulated NF-kappaB activation. Carbachol 74-77 nuclear factor kappa B subunit 1 Homo sapiens 89-98 19017493-3 2008 Treatment of the four cell lines with the cholinergic agonist carbachol led to the activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2). Carbachol 62-71 mitogen-activated protein kinase 1 Homo sapiens 97-143 19017493-3 2008 Treatment of the four cell lines with the cholinergic agonist carbachol led to the activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2). Carbachol 62-71 mitogen-activated protein kinase 3 Homo sapiens 145-151 19017493-4 2008 In SNU-407 cells, carbachol significantly stimulated cell proliferation, which could be abolished by the muscarinic antagonist atropine and the ERK1/2 kinase inhibitor PD98059. Carbachol 18-27 mitogen-activated protein kinase 3 Homo sapiens 144-150 18755685-4 2008 Carbachol (CCh) stimulation of HEK293 cells expressing wild type TRPC3 induced recruitment of a ternary TRPC3-RACK1-IP(3)R complex and increased surface expression of TRPC3 and Ca(2+) entry. Carbachol 0-9 transient receptor potential cation channel subfamily C member 3 Homo sapiens 104-109 18755685-4 2008 Carbachol (CCh) stimulation of HEK293 cells expressing wild type TRPC3 induced recruitment of a ternary TRPC3-RACK1-IP(3)R complex and increased surface expression of TRPC3 and Ca(2+) entry. Carbachol 0-9 receptor for activated C kinase 1 Homo sapiens 110-115 18755685-4 2008 Carbachol (CCh) stimulation of HEK293 cells expressing wild type TRPC3 induced recruitment of a ternary TRPC3-RACK1-IP(3)R complex and increased surface expression of TRPC3 and Ca(2+) entry. Carbachol 0-9 inositol 1,4,5-trisphosphate receptor type 3 Homo sapiens 116-122 18755685-4 2008 Carbachol (CCh) stimulation of HEK293 cells expressing wild type TRPC3 induced recruitment of a ternary TRPC3-RACK1-IP(3)R complex and increased surface expression of TRPC3 and Ca(2+) entry. Carbachol 0-9 transient receptor potential cation channel subfamily C member 3 Homo sapiens 104-109 18755685-4 2008 Carbachol (CCh) stimulation of HEK293 cells expressing wild type TRPC3 induced recruitment of a ternary TRPC3-RACK1-IP(3)R complex and increased surface expression of TRPC3 and Ca(2+) entry. Carbachol 11-14 transient receptor potential cation channel subfamily C member 3 Homo sapiens 65-70 18755685-4 2008 Carbachol (CCh) stimulation of HEK293 cells expressing wild type TRPC3 induced recruitment of a ternary TRPC3-RACK1-IP(3)R complex and increased surface expression of TRPC3 and Ca(2+) entry. Carbachol 11-14 transient receptor potential cation channel subfamily C member 3 Homo sapiens 104-109 18755685-4 2008 Carbachol (CCh) stimulation of HEK293 cells expressing wild type TRPC3 induced recruitment of a ternary TRPC3-RACK1-IP(3)R complex and increased surface expression of TRPC3 and Ca(2+) entry. Carbachol 11-14 receptor for activated C kinase 1 Homo sapiens 110-115 18755685-4 2008 Carbachol (CCh) stimulation of HEK293 cells expressing wild type TRPC3 induced recruitment of a ternary TRPC3-RACK1-IP(3)R complex and increased surface expression of TRPC3 and Ca(2+) entry. Carbachol 11-14 inositol 1,4,5-trisphosphate receptor type 3 Homo sapiens 116-122 18755685-4 2008 Carbachol (CCh) stimulation of HEK293 cells expressing wild type TRPC3 induced recruitment of a ternary TRPC3-RACK1-IP(3)R complex and increased surface expression of TRPC3 and Ca(2+) entry. Carbachol 11-14 transient receptor potential cation channel subfamily C member 3 Homo sapiens 104-109 18755685-6 2008 CCh-stimulated recruitment of TRPC3-RACK1-IP(3)R complex as well as increased surface expression of TRPC3 and receptor-operated Ca(2+) entry were also attenuated. Carbachol 0-3 transient receptor potential cation channel subfamily C member 3 Homo sapiens 30-35 18755685-6 2008 CCh-stimulated recruitment of TRPC3-RACK1-IP(3)R complex as well as increased surface expression of TRPC3 and receptor-operated Ca(2+) entry were also attenuated. Carbachol 0-3 receptor for activated C kinase 1 Homo sapiens 36-41 18755685-6 2008 CCh-stimulated recruitment of TRPC3-RACK1-IP(3)R complex as well as increased surface expression of TRPC3 and receptor-operated Ca(2+) entry were also attenuated. Carbachol 0-3 inositol 1,4,5-trisphosphate receptor type 3 Homo sapiens 42-48 18755685-6 2008 CCh-stimulated recruitment of TRPC3-RACK1-IP(3)R complex as well as increased surface expression of TRPC3 and receptor-operated Ca(2+) entry were also attenuated. Carbachol 0-3 transient receptor potential cation channel subfamily C member 3 Homo sapiens 100-105 18755685-8 2008 Knockdown of endogenous TRPC3 also decreased RACK1-IP(3)R association and decreased CCh-stimulated Ca(2+) entry. Carbachol 84-87 transient receptor potential cation channel subfamily C member 3 Homo sapiens 24-29 18755685-10 2008 Similar oscillatory pattern of Ca(2+) release was seen after CCh stimulation of cells expressing the TRPC3 mutant. Carbachol 61-64 transient receptor potential cation channel subfamily C member 3 Homo sapiens 101-106 18755690-8 2008 Exposure of astrocytes to carbachol increased the expression of the extracellular matrix proteins fibronectin and laminin-1 in these cells. Carbachol 26-35 fibronectin 1 Rattus norvegicus 98-109 18755690-8 2008 Exposure of astrocytes to carbachol increased the expression of the extracellular matrix proteins fibronectin and laminin-1 in these cells. Carbachol 26-35 laminin subunit alpha 1 Rattus norvegicus 114-123 18755690-10 2008 The inhibition of fibronectin activity strongly reduced the effect of carbachol on the elongation of all the neurites, whereas inhibition of laminin-1 activity reduced the elongation of minor neurites only. Carbachol 70-79 fibronectin 1 Rattus norvegicus 18-29 18768927-3 2008 The present study demonstrates that chronic exposure of polarized rat parotid gland (Par-C10) epithelial cell monolayers to TNF-alpha and IFN-gamma decreases transepithelial resistance (TER) and anion secretion, as measured by changes in short-circuit current (I(sc)) induced by carbachol, a muscarinic cholinergic receptor agonist, or UTP, a P2Y(2) nucleotide receptor agonist. Carbachol 279-288 tumor necrosis factor Rattus norvegicus 124-133 18768927-3 2008 The present study demonstrates that chronic exposure of polarized rat parotid gland (Par-C10) epithelial cell monolayers to TNF-alpha and IFN-gamma decreases transepithelial resistance (TER) and anion secretion, as measured by changes in short-circuit current (I(sc)) induced by carbachol, a muscarinic cholinergic receptor agonist, or UTP, a P2Y(2) nucleotide receptor agonist. Carbachol 279-288 interferon gamma Rattus norvegicus 138-147 18929546-5 2008 The three ligands all induced a reversible increase in firing, however, only carbachol-induced increase in firing was attenuated by the PKC inhibitors chelerythrine or GF 109203X. Carbachol 77-86 proline rich transmembrane protein 2 Homo sapiens 136-139 18929546-9 2008 Furthermore, preincubation with the PKC inhibitor GF 109203X reduced the carbachol-induced increase in nifedipine-sensitive high-voltage gated calcium currents. Carbachol 73-82 proline rich transmembrane protein 2 Homo sapiens 36-39 18617156-7 2008 Difference between basal release and carbachol-induced secretion achieved statistical significance as to all the proteins/peptides under study but for statherin. Carbachol 37-46 statherin Homo sapiens 151-160 18815229-8 2008 Carbachol and PMA likewise caused redistribution of NBCe1 from BLM to early endosomes. Carbachol 0-9 solute carrier family 4 member 4 S homeolog Xenopus laevis 52-57 18755685-4 2008 Carbachol (CCh) stimulation of HEK293 cells expressing wild type TRPC3 induced recruitment of a ternary TRPC3-RACK1-IP(3)R complex and increased surface expression of TRPC3 and Ca(2+) entry. Carbachol 0-9 transient receptor potential cation channel subfamily C member 3 Homo sapiens 65-70 18567601-6 2008 In parietal cells of cultured gastric glands from wild-type mice treated with gastrin, histamine or carbachol, ezrin was localized to vesicular structures resembling secretory canaliculi. Carbachol 100-109 ezrin Mus musculus 111-116 18772167-6 2008 Brief exposure of INGAP-PP-treated islets to carbachol (Cch) significantly increased P70S6K(-Thr389) and MAPK3/1 phosphorylation and these islets released more insulin when challenged with Cch that was prevented by the M3 receptor antagonist 4-DAMP, in a concentration-dependent manner. Carbachol 45-54 ribosomal protein S6 kinase B1 Rattus norvegicus 85-91 18687805-2 2008 Thrombin also activates Ras homolog gene family member A (RhoA) and activating protein (AP-1) -mediated gene expression in 1321N1 astrocytoma cells, whereas the nonmitogenic agonist carbachol does not. Carbachol 182-191 coagulation factor II, thrombin Homo sapiens 0-8 18722532-5 2008 First we show that the PDBu- and carbachol-stimulated N1 secretions are blocked by the general PKC inhibitor GF109203X. Carbachol 33-42 protein kinase C alpha Homo sapiens 95-98 18772167-6 2008 Brief exposure of INGAP-PP-treated islets to carbachol (Cch) significantly increased P70S6K(-Thr389) and MAPK3/1 phosphorylation and these islets released more insulin when challenged with Cch that was prevented by the M3 receptor antagonist 4-DAMP, in a concentration-dependent manner. Carbachol 45-54 mitogen activated protein kinase 3 Rattus norvegicus 105-110 18772167-6 2008 Brief exposure of INGAP-PP-treated islets to carbachol (Cch) significantly increased P70S6K(-Thr389) and MAPK3/1 phosphorylation and these islets released more insulin when challenged with Cch that was prevented by the M3 receptor antagonist 4-DAMP, in a concentration-dependent manner. Carbachol 56-59 ribosomal protein S6 kinase B1 Rattus norvegicus 85-91 18772167-6 2008 Brief exposure of INGAP-PP-treated islets to carbachol (Cch) significantly increased P70S6K(-Thr389) and MAPK3/1 phosphorylation and these islets released more insulin when challenged with Cch that was prevented by the M3 receptor antagonist 4-DAMP, in a concentration-dependent manner. Carbachol 56-59 mitogen activated protein kinase 3 Rattus norvegicus 105-110 18774166-4 2008 METHODS: beta(2)-AR responsiveness to isoproterenol was characterized in human precision-cut lung slices (PCLSs) precontracted to carbachol after pretreatment with albuterol. Carbachol 130-139 adrenoceptor beta 2 Homo sapiens 9-19 18828925-13 2008 Double labeling demonstrated that a number of AVP- and OT-containing neurons in the fetal SON and PVN were expressing c-fos in response to central carbachol. Carbachol 147-156 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 118-123 18602908-0 2008 Monitoring the reaction of carbachol with acetylcholinesterase by thioflavin T fluorescence and acetylthiocholine hydrolysis. Carbachol 27-36 acetylcholinesterase (Cartwright blood group) Homo sapiens 42-62 18602908-5 2008 Here we show that the reaction of carbachol (carbamoylcholine) with AChE can be monitored both with acetylthiocholine as a reporter substrate and with thioflavin T as a fluorescent reporter group. Carbachol 34-43 acetylcholinesterase (Cartwright blood group) Homo sapiens 68-72 18602908-5 2008 Here we show that the reaction of carbachol (carbamoylcholine) with AChE can be monitored both with acetylthiocholine as a reporter substrate and with thioflavin T as a fluorescent reporter group. Carbachol 45-61 acetylcholinesterase (Cartwright blood group) Homo sapiens 68-72 18524939-4 2008 The dependence of force on MLCK activity was nonlinear such that at higher concentrations of CCh, force increased with no change in the net 20% activation of MLCK. Carbachol 93-96 myosin light chain kinase 3 Mus musculus 27-31 18632735-1 2008 We previously found that the phosphorylation of ERK1/2 by submaximal concentrations of the muscarinic receptor ligand carbachol was potentiated in rat parotid acinar cells exposed to ouabain, a cardiac glycoside that inhibits the Na-K-ATPase. Carbachol 118-127 mitogen activated protein kinase 3 Rattus norvegicus 48-54 18758068-4 2008 TRPC5 was initially activated by muscarinic stimulation using 50 microM carbachol (CCh) and decayed rapidly in the presence of CCh (desensitization). Carbachol 72-81 transient receptor potential cation channel subfamily C member 5 Homo sapiens 0-5 18758068-4 2008 TRPC5 was initially activated by muscarinic stimulation using 50 microM carbachol (CCh) and decayed rapidly in the presence of CCh (desensitization). Carbachol 83-86 transient receptor potential cation channel subfamily C member 5 Homo sapiens 0-5 18758068-4 2008 TRPC5 was initially activated by muscarinic stimulation using 50 microM carbachol (CCh) and decayed rapidly in the presence of CCh (desensitization). Carbachol 127-130 transient receptor potential cation channel subfamily C member 5 Homo sapiens 0-5 18758068-11 2008 In mouse ileal myocytes, the desensitization of CCh-activated inward current (I(CCh)) also slowed in the presence of PIP(2) in recording pipettes. Carbachol 48-51 prolactin induced protein Mus musculus 117-120 18758068-11 2008 In mouse ileal myocytes, the desensitization of CCh-activated inward current (I(CCh)) also slowed in the presence of PIP(2) in recording pipettes. Carbachol 80-83 prolactin induced protein Mus musculus 117-120 18627437-4 2008 Stable and transient depletion of CIB1 by short-hairpin RNA increased the Ca2+ response of HEK293 cells to the InsP3-generating ligands ATP, UTP and carbachol. Carbachol 149-158 calcium and integrin binding 1 Homo sapiens 34-38 18632735-8 2008 These results suggest that carbachol-initiated AMPK activation can produce a negative feedback on ERK1/2 signaling in response to submaximal muscarinic receptor activation and that increases in fluid secretion can modulate receptor-initiated signaling events indirectly by producing ion transport-dependent decreases in ATP. Carbachol 27-36 mitogen activated protein kinase 3 Rattus norvegicus 98-104 18617565-2 2008 Cationic currents due to TRPC6 expression were strongly suppressed (by approximately 70%) by a NO donor SNAP (100 microm) whether it was applied prior to muscarinic receptor stimulation with carbachol (CCh; 100 microm) or after G-protein activation with intracellular perfusion of GTPgammaS (100 microm). Carbachol 191-200 transient receptor potential cation channel subfamily C member 6 Homo sapiens 25-30 18617565-2 2008 Cationic currents due to TRPC6 expression were strongly suppressed (by approximately 70%) by a NO donor SNAP (100 microm) whether it was applied prior to muscarinic receptor stimulation with carbachol (CCh; 100 microm) or after G-protein activation with intracellular perfusion of GTPgammaS (100 microm). Carbachol 202-205 transient receptor potential cation channel subfamily C member 6 Homo sapiens 25-30 18577515-5 2008 Stimulation with cholecystokinin (CCK), carbachol, and vasoactive intestinal peptide all induced Rap1 activation, as did calcium ionophore A23187, phorbol ester, forskolin, 8-bromo-cyclic AMP, and the Epac-specific cAMP analog 8-pCPT-2"-O-Me-cAMP. Carbachol 40-49 RAS-related protein 1a Mus musculus 97-101 18577515-5 2008 Stimulation with cholecystokinin (CCK), carbachol, and vasoactive intestinal peptide all induced Rap1 activation, as did calcium ionophore A23187, phorbol ester, forskolin, 8-bromo-cyclic AMP, and the Epac-specific cAMP analog 8-pCPT-2"-O-Me-cAMP. Carbachol 40-49 Rap guanine nucleotide exchange factor (GEF) 3 Mus musculus 201-205 18577515-7 2008 Co-stimulation with carbachol and 8-pCPT-2"-O-Me-cAMP led to an additive effect on Rap1 activation, whereas a synergistic effect was seen on amylase release. Carbachol 20-29 RAS-related protein 1a Mus musculus 83-87 18577515-9 2008 Overexpression of Rap1 GTPase-activating protein, which blocked Rap1 activation, reduced the effect of 8-bromo-cyclic AMP, 8-pCPT-2"-O-Me-cAMP, and vasoactive intestinal peptide on amylase release by 60% and reduced CCK- as well as carbachol-stimulated pancreatic amylase release by 40%. Carbachol 232-241 RAS-related protein 1a Mus musculus 18-22 18577515-9 2008 Overexpression of Rap1 GTPase-activating protein, which blocked Rap1 activation, reduced the effect of 8-bromo-cyclic AMP, 8-pCPT-2"-O-Me-cAMP, and vasoactive intestinal peptide on amylase release by 60% and reduced CCK- as well as carbachol-stimulated pancreatic amylase release by 40%. Carbachol 232-241 RAS-related protein 1a Mus musculus 64-68 18577515-9 2008 Overexpression of Rap1 GTPase-activating protein, which blocked Rap1 activation, reduced the effect of 8-bromo-cyclic AMP, 8-pCPT-2"-O-Me-cAMP, and vasoactive intestinal peptide on amylase release by 60% and reduced CCK- as well as carbachol-stimulated pancreatic amylase release by 40%. Carbachol 232-241 cholecystokinin Mus musculus 216-219 18577515-11 2008 Rap1 activation not only mediates the cAMP-evoked response via Epac1 but is also involved in CCK- and carbachol-induced amylase release, with their action most likely mediated by CalDAG-GEF III. Carbachol 102-111 RAS-related protein 1a Mus musculus 0-4 18658048-8 2008 Finally, RGS4-null SAN cells showed decreased levels of G protein-coupled inward rectifying potassium (GIRK) channel desensitization and altered modulation of acetylcholine-sensitive potassium current (I(KACh)) kinetics following carbachol stimulation. Carbachol 230-239 regulator of G-protein signaling 4 Mus musculus 9-13 18524858-10 2008 The decrease in cGMP response to carbamyl-choline (eNOS agonist) was significantly attenuated by rosiglitazone in CRF. Carbachol 33-49 nitric oxide synthase 3 Rattus norvegicus 51-55 18524939-7 2008 CCh treatment, but not KCl, resulted in MYPT1 and CPI-17 phosphorylation. Carbachol 0-3 protein phosphatase 1, regulatory subunit 12A Mus musculus 40-45 18524939-7 2008 CCh treatment, but not KCl, resulted in MYPT1 and CPI-17 phosphorylation. Carbachol 0-3 protein phosphatase 1, regulatory inhibitor subunit 14A Mus musculus 50-56 18524939-8 2008 Both Y27632 (Rho-kinase inhibitor) and calphostin C (PKC inhibitor) reduced CCh-dependent force, RLC phosphorylation, and phosphorylation of MYPT1 (Thr694) without changing MLCK activation. Carbachol 76-79 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 13-23 18524939-8 2008 Both Y27632 (Rho-kinase inhibitor) and calphostin C (PKC inhibitor) reduced CCh-dependent force, RLC phosphorylation, and phosphorylation of MYPT1 (Thr694) without changing MLCK activation. Carbachol 76-79 myosin light chain kinase 3 Mus musculus 173-177 18524939-9 2008 Calphostin C, but not Y27632, also reduced CCh-induced phosphorylation of CPI-17. Carbachol 43-46 protein phosphatase 1, regulatory inhibitor subunit 14A Mus musculus 74-80 18524939-10 2008 CCh concentration responses showed that phosphorylation of CPI-17 was more sensitive than MYPT1. Carbachol 0-3 protein phosphatase 1, regulatory inhibitor subunit 14A Mus musculus 59-65 18524939-10 2008 CCh concentration responses showed that phosphorylation of CPI-17 was more sensitive than MYPT1. Carbachol 0-3 protein phosphatase 1, regulatory subunit 12A Mus musculus 90-95 18300276-5 2008 Among agents tested, carbachol and gastrin were strong inhibitors of RELMbeta mRNA accumulation. Carbachol 21-30 resistin like beta Homo sapiens 69-77 18348264-4 2008 In SK-N-SH cells, the acetylcholine analog carbachol stimulated phosphorylation of the ribosomal S6 protein, a downstream target of mTOR. Carbachol 43-52 mechanistic target of rapamycin kinase Homo sapiens 132-136 18348264-6 2008 Carbachol-evoked S6 phosphorylation was blocked by the mTOR inhibitor rapamycin, but was independent of phosphoinositide 3-kinase activation. Carbachol 0-9 mechanistic target of rapamycin kinase Homo sapiens 55-59 18434623-4 2008 Upon stimulation of secretion with the muscarinic agonist, carbachol, Myo5c was also detected in association with actin-coated fusion intermediates. Carbachol 59-68 unconventional myosin-Vc Oryctolagus cuniculus 70-75 18434623-8 2008 These studies revealed that the carbachol-stimulated increase in secretory vesicle diameter associated with compound fusion of secretory vesicles that was also exhibited by vesicles labeled with GFP-Myo5c-full was impaired in vesicles labeled with GFP-Myo5c-tail. Carbachol 32-41 unconventional myosin-Vc Oryctolagus cuniculus 199-204 18434623-8 2008 These studies revealed that the carbachol-stimulated increase in secretory vesicle diameter associated with compound fusion of secretory vesicles that was also exhibited by vesicles labeled with GFP-Myo5c-full was impaired in vesicles labeled with GFP-Myo5c-tail. Carbachol 32-41 unconventional myosin-Vc Oryctolagus cuniculus 252-257 18434623-9 2008 A significant decrease in GFP labeling of actin-coated fusion intermediates was also seen in carbachol-stimulated LGAC transduced with GFP-Myo5c-tail relative to LGAC transduced with GFP-Myo5c-full. Carbachol 93-102 unconventional myosin-Vc Oryctolagus cuniculus 139-144 18434623-9 2008 A significant decrease in GFP labeling of actin-coated fusion intermediates was also seen in carbachol-stimulated LGAC transduced with GFP-Myo5c-tail relative to LGAC transduced with GFP-Myo5c-full. Carbachol 93-102 unconventional myosin-Vc Oryctolagus cuniculus 187-192 18316702-10 2008 mRNA and protein for TRPC1 and TRPC6 were present in mouse Muller cells, and carbachol activated a Gd(3+)-sensitive, TRP-like cation channel. Carbachol 77-86 transient receptor potential cation channel, subfamily C, member 1 Mus musculus 21-26 18436530-2 2008 Here we demonstrate that the muscarinic receptor agonist carbachol activates AMPKalpha1-containing complexes in the human SH-SY5Y cell line via a mechanism specific for the AMPK upstream kinase, Ca(2+)/calmodulin-dependent protein kinase kinase beta. Carbachol 57-66 protein kinase AMP-activated catalytic subunit alpha 1 Homo sapiens 77-87 18436530-2 2008 Here we demonstrate that the muscarinic receptor agonist carbachol activates AMPKalpha1-containing complexes in the human SH-SY5Y cell line via a mechanism specific for the AMPK upstream kinase, Ca(2+)/calmodulin-dependent protein kinase kinase beta. Carbachol 57-66 protein kinase AMP-activated catalytic subunit alpha 1 Homo sapiens 77-81 18388243-3 2008 We show that knockdown of GRK2, GRK3, or GRK6, but not GRK5, significantly increased carbachol-mediated calcium mobilization. Carbachol 85-94 G protein-coupled receptor kinase 2 Homo sapiens 26-30 18388243-6 2008 Knockdown of GRK2 and the arrestins also significantly enhanced carbachol-mediated activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2), whereas prolonged ERK1/2 activation was only observed with GRK2 or arrestin-3 knockdown. Carbachol 64-73 G protein-coupled receptor kinase 2 Homo sapiens 13-17 18385290-0 2008 Distinct pathways of ERK activation by the muscarinic agonists pilocarpine and carbachol in a human salivary cell line. Carbachol 79-88 mitogen-activated protein kinase 1 Homo sapiens 21-24 18385290-2 2008 We examined the activation of ERK1/2 by two muscarinic agonists, pilocarpine and carbachol, in a human salivary cell line (HSY). Carbachol 81-90 mitogen-activated protein kinase 3 Homo sapiens 30-36 18385290-6 2008 Downregulation of PKC by prolonged treatment of cells with the phorbol ester PMA diminished carbachol-induced ERK phosphorylation but had no effect on pilocarpine responsiveness. Carbachol 92-101 mitogen-activated protein kinase 1 Homo sapiens 110-113 18385290-12 2008 Our results demonstrate that the actions of pilocarpine and carbachol in salivary cells are mediated through two distinct signaling mechanisms-pilocarpine acting via M3 receptors and Src-dependent transactivation of EGF receptors, and carbachol via M1/M3 receptors and PKC-converging on the ERK pathway. Carbachol 60-69 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 183-186 18385290-12 2008 Our results demonstrate that the actions of pilocarpine and carbachol in salivary cells are mediated through two distinct signaling mechanisms-pilocarpine acting via M3 receptors and Src-dependent transactivation of EGF receptors, and carbachol via M1/M3 receptors and PKC-converging on the ERK pathway. Carbachol 60-69 mitogen-activated protein kinase 1 Homo sapiens 291-294 18388188-5 2008 The apical-to-basal carbachol (1 muM)-stimulated Ca(2+) wave was 8.63 +/- 0.68 microm/s; it increased to 19.66 +/- 2.22 microm/s (*P < 0.0005) with VIP (100 nM), and similar increases were observed with 8-Br-cAMP (100 microM). Carbachol 20-29 vasoactive intestinal peptide Rattus norvegicus 151-154 18437352-8 2008 Weak, Ca(2+)-dependent stimulation with ionomycin or carbachol induced exclusive release of chromogranin B, suggesting a higher Ca(2+) sensitivity of the specific granules. Carbachol 53-62 chromogranin B Homo sapiens 92-106 18440028-0 2008 K+ACh channel activation with carbachol increases atrial ANP release. Carbachol 30-39 natriuretic peptides A Oryctolagus cuniculus 57-60 18298664-4 2008 The mixed nAChR-mAChR agonists acetylcholine (ACh) and carbachol provoked [(3)H]DA release partially sensitive to the mAChR antagonist atropine but totally blocked by the nAChR antagonist mecamylamine. Carbachol 55-64 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 10-15 18298664-4 2008 The mixed nAChR-mAChR agonists acetylcholine (ACh) and carbachol provoked [(3)H]DA release partially sensitive to the mAChR antagonist atropine but totally blocked by the nAChR antagonist mecamylamine. Carbachol 55-64 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 171-176 18440028-4 2008 Carbachol (CCh), an agonist of cardiac mAChR, increased atrial myocyte ANP release concomitantly with a decrease in stroke volume and intra-atrial pulse pressure in a concentration-dependent manner. Carbachol 0-9 natriuretic peptides A Oryctolagus cuniculus 71-74 18440028-4 2008 Carbachol (CCh), an agonist of cardiac mAChR, increased atrial myocyte ANP release concomitantly with a decrease in stroke volume and intra-atrial pulse pressure in a concentration-dependent manner. Carbachol 11-14 natriuretic peptides A Oryctolagus cuniculus 71-74 18440028-6 2008 In the presence of isoproterenol, the CCh-induced increase in ANP release and decrease in cAMP efflux levels and mechanical dynamics were able to be repeated. Carbachol 38-41 natriuretic peptides A Oryctolagus cuniculus 62-65 18440028-8 2008 Tertiapin, a selective G-protein-gated K(+)(ACh) channel blocker, attenuated the CCh-induced increase in ANP release and decrease in mechanical dynamics in a concentration-dependent manner, but without a significant effect on the CCh-induced decrease in cAMP efflux levels. Carbachol 81-84 natriuretic peptides A Oryctolagus cuniculus 105-108 18440028-9 2008 The CCh-induced changes in ANP release and atrial dynamics were inhibited in the atria from pertussis toxin-pretreated rabbits. Carbachol 4-7 natriuretic peptides A Oryctolagus cuniculus 27-30 18406062-9 2008 Furthermore activation of the ERK pathway by ex vivo cholinergic stimulation with carbachol caused significantly higher activation of ERK in the hippocampus of Wt mice than in Tg mice. Carbachol 82-91 mitogen-activated protein kinase 1 Mus musculus 30-33 18406062-9 2008 Furthermore activation of the ERK pathway by ex vivo cholinergic stimulation with carbachol caused significantly higher activation of ERK in the hippocampus of Wt mice than in Tg mice. Carbachol 82-91 mitogen-activated protein kinase 1 Mus musculus 134-137 18204473-6 2008 KEY RESULTS: TRPC5 activation by carbachol, Gd3+ or LPC was inhibited by halothane and chloroform at > or =0.1 and 0.2 mM respectively. Carbachol 33-42 transient receptor potential cation channel subfamily C member 5 Homo sapiens 13-18 18434353-5 2008 Activation of the endogenously expressed M3 receptor by the cholinergic agonist carbachol also potentiated CRH-induced insulin secretion, indicating that the phenomenon may be pathway specific (i.e. Ca2+-phospholipase C) rather than agonist specific. Carbachol 80-89 corticotropin releasing hormone Mus musculus 107-110 18171724-7 2008 Adenovirus-mediated overexpression of mutant Rab3DT36N in LGACs inhibited CCH-stimulated SC release, and, in CCH-stimulated LGACs, pull down of pIgR with Rab3DWT and colocalization of pIgR with endogenous Rab3D were decreased relative to resting cells, suggesting that the pIgR-Rab3D interaction may be modulated by secretagogues. Carbachol 74-77 ras-related protein Rab-3D Oryctolagus cuniculus 45-50 18171724-7 2008 Adenovirus-mediated overexpression of mutant Rab3DT36N in LGACs inhibited CCH-stimulated SC release, and, in CCH-stimulated LGACs, pull down of pIgR with Rab3DWT and colocalization of pIgR with endogenous Rab3D were decreased relative to resting cells, suggesting that the pIgR-Rab3D interaction may be modulated by secretagogues. Carbachol 109-112 polymeric immunoglobulin receptor Oryctolagus cuniculus 144-148 18171724-7 2008 Adenovirus-mediated overexpression of mutant Rab3DT36N in LGACs inhibited CCH-stimulated SC release, and, in CCH-stimulated LGACs, pull down of pIgR with Rab3DWT and colocalization of pIgR with endogenous Rab3D were decreased relative to resting cells, suggesting that the pIgR-Rab3D interaction may be modulated by secretagogues. Carbachol 109-112 ras-related protein Rab-3D Oryctolagus cuniculus 45-50 18457815-7 2008 In another set of experiments, the specific NK1 receptor antagonist L-703,606 (5 microg) was microinjected in the RVM following LH stimulation with carbachol and abolished LH-induced antinociception as well. Carbachol 148-157 tachykinin receptor 1 Rattus norvegicus 44-56 18276774-4 2008 Tracheal smooth muscle contractility is enhanced by overnight incubation with IL-13, resulting in increased maximal contractions (E(max)) to carbachol (CCh) and KCl. Carbachol 141-150 interleukin 13 Mus musculus 78-83 18276774-4 2008 Tracheal smooth muscle contractility is enhanced by overnight incubation with IL-13, resulting in increased maximal contractions (E(max)) to carbachol (CCh) and KCl. Carbachol 152-155 interleukin 13 Mus musculus 78-83 18379433-6 2008 Carbachol-stimulated release of intracellular calcium is significantly higher in cells expressing N-terminal mutant huntingtin and TG2 compared with vector-transfected cells; expression of either CaM-center or CaM-overlap in these cells returned the levels of carbachol-stimulated intracellular calcium release to control values. Carbachol 0-9 huntingtin Homo sapiens 116-126 18379433-6 2008 Carbachol-stimulated release of intracellular calcium is significantly higher in cells expressing N-terminal mutant huntingtin and TG2 compared with vector-transfected cells; expression of either CaM-center or CaM-overlap in these cells returned the levels of carbachol-stimulated intracellular calcium release to control values. Carbachol 0-9 calmodulin 1 Homo sapiens 196-199 18379433-6 2008 Carbachol-stimulated release of intracellular calcium is significantly higher in cells expressing N-terminal mutant huntingtin and TG2 compared with vector-transfected cells; expression of either CaM-center or CaM-overlap in these cells returned the levels of carbachol-stimulated intracellular calcium release to control values. Carbachol 0-9 calmodulin 1 Homo sapiens 210-213 18379433-6 2008 Carbachol-stimulated release of intracellular calcium is significantly higher in cells expressing N-terminal mutant huntingtin and TG2 compared with vector-transfected cells; expression of either CaM-center or CaM-overlap in these cells returned the levels of carbachol-stimulated intracellular calcium release to control values. Carbachol 260-269 calmodulin 1 Homo sapiens 196-199 18379433-6 2008 Carbachol-stimulated release of intracellular calcium is significantly higher in cells expressing N-terminal mutant huntingtin and TG2 compared with vector-transfected cells; expression of either CaM-center or CaM-overlap in these cells returned the levels of carbachol-stimulated intracellular calcium release to control values. Carbachol 260-269 calmodulin 1 Homo sapiens 210-213 17581530-8 2008 Abeta 1-40 in absence of neurotoxicity fully inhibited the DA release evoked by CCh and only marginally affected the DA release evoked by epibatidine. Carbachol 80-83 amyloid beta precursor protein Homo sapiens 0-5 17581530-9 2008 The PKC inhibitor GF109203X mimicked the effect of Abeta on DA release and, in turn, Abeta impaired PKC activation by CCh. Carbachol 118-121 amyloid beta precursor protein Homo sapiens 51-56 18155849-0 2008 Fos expression in pontomedullary catecholaminergic cells following rapid eye movement sleep-like episodes elicited by pontine carbachol in urethane-anesthetized rats. Carbachol 126-135 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 0-3 18155849-5 2008 The percentage of Fos-positive TH cells was negatively correlated with the cumulative duration of REMS-like episodes induced during 140 min prior to brain harvesting in the A7 and rostral A5 groups bilaterally (P < 0.01 for both), and in SubC neurons on the side opposite to carbachol injection (P < 0.05). Carbachol 278-287 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 18-21 18344611-2 2008 In the present study, we showed that a muscarinic receptor agonist, carbachol (CCh), activates almost 20% of orexin-producing neurons (orexin neurons), which play a critical role in maintenance of arousal. Carbachol 68-77 hypocretin Mus musculus 109-115 18344611-2 2008 In the present study, we showed that a muscarinic receptor agonist, carbachol (CCh), activates almost 20% of orexin-producing neurons (orexin neurons), which play a critical role in maintenance of arousal. Carbachol 68-77 hypocretin Mus musculus 135-141 18344611-2 2008 In the present study, we showed that a muscarinic receptor agonist, carbachol (CCh), activates almost 20% of orexin-producing neurons (orexin neurons), which play a critical role in maintenance of arousal. Carbachol 79-82 hypocretin Mus musculus 109-115 18344611-2 2008 In the present study, we showed that a muscarinic receptor agonist, carbachol (CCh), activates almost 20% of orexin-producing neurons (orexin neurons), which play a critical role in maintenance of arousal. Carbachol 79-82 hypocretin Mus musculus 135-141 18344611-3 2008 We also found that a very small population of orexin neurons (1%) was inhibited by CCh. Carbachol 83-86 hypocretin Mus musculus 46-52 18344611-4 2008 Muscarinic receptor antagonists inhibited the CCh-induced activation of orexin neurons in a dose-dependent manner. Carbachol 46-49 hypocretin Mus musculus 72-78 18344611-8 2008 These results indicate that CCh activates 20% of orexin neurons through the M(3) muscarinic receptor and subsequent activation of nonselective cation channels. Carbachol 28-31 hypocretin Mus musculus 49-55 18155321-2 2008 Here, the effect of the muscarinic agonist carbachol on NPY biosynthesis and release was analyzed utilizing the SH-SY5Y human neuroblastoma cell line. Carbachol 43-52 neuropeptide Y Homo sapiens 56-59 18310132-7 2008 Using local photolysis of caged carbachol, a broad-spectrum cholinergic agonist, we mapped alpha7 nAChR-mediated currents along the visible extent of filled SNr neurons and found that alpha7 nAChRs can be functionally detected as far as 60 microm away from the soma. Carbachol 32-41 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 98-103 18155321-3 2008 We observed that: (a) carbachol moderately increased the post-translational cleavage of proNPY to NPY; (b) carbachol treatment stimulated NPY accumulation into the medium in a time- and dose-related manner; (c) protein kinase C activation is involved in carbachol-mediated NPY synthesis/release (>6h). Carbachol 22-31 neuropeptide Y Homo sapiens 91-94 18155321-3 2008 We observed that: (a) carbachol moderately increased the post-translational cleavage of proNPY to NPY; (b) carbachol treatment stimulated NPY accumulation into the medium in a time- and dose-related manner; (c) protein kinase C activation is involved in carbachol-mediated NPY synthesis/release (>6h). Carbachol 22-31 neuropeptide Y Homo sapiens 98-101 18155321-3 2008 We observed that: (a) carbachol moderately increased the post-translational cleavage of proNPY to NPY; (b) carbachol treatment stimulated NPY accumulation into the medium in a time- and dose-related manner; (c) protein kinase C activation is involved in carbachol-mediated NPY synthesis/release (>6h). Carbachol 22-31 neuropeptide Y Homo sapiens 98-101 18155321-3 2008 We observed that: (a) carbachol moderately increased the post-translational cleavage of proNPY to NPY; (b) carbachol treatment stimulated NPY accumulation into the medium in a time- and dose-related manner; (c) protein kinase C activation is involved in carbachol-mediated NPY synthesis/release (>6h). Carbachol 107-116 neuropeptide Y Homo sapiens 91-94 18155321-3 2008 We observed that: (a) carbachol moderately increased the post-translational cleavage of proNPY to NPY; (b) carbachol treatment stimulated NPY accumulation into the medium in a time- and dose-related manner; (c) protein kinase C activation is involved in carbachol-mediated NPY synthesis/release (>6h). Carbachol 107-116 neuropeptide Y Homo sapiens 98-101 18155321-3 2008 We observed that: (a) carbachol moderately increased the post-translational cleavage of proNPY to NPY; (b) carbachol treatment stimulated NPY accumulation into the medium in a time- and dose-related manner; (c) protein kinase C activation is involved in carbachol-mediated NPY synthesis/release (>6h). Carbachol 107-116 neuropeptide Y Homo sapiens 98-101 18155321-3 2008 We observed that: (a) carbachol moderately increased the post-translational cleavage of proNPY to NPY; (b) carbachol treatment stimulated NPY accumulation into the medium in a time- and dose-related manner; (c) protein kinase C activation is involved in carbachol-mediated NPY synthesis/release (>6h). Carbachol 107-116 neuropeptide Y Homo sapiens 91-94 18155321-3 2008 We observed that: (a) carbachol moderately increased the post-translational cleavage of proNPY to NPY; (b) carbachol treatment stimulated NPY accumulation into the medium in a time- and dose-related manner; (c) protein kinase C activation is involved in carbachol-mediated NPY synthesis/release (>6h). Carbachol 107-116 neuropeptide Y Homo sapiens 98-101 18155321-3 2008 We observed that: (a) carbachol moderately increased the post-translational cleavage of proNPY to NPY; (b) carbachol treatment stimulated NPY accumulation into the medium in a time- and dose-related manner; (c) protein kinase C activation is involved in carbachol-mediated NPY synthesis/release (>6h). Carbachol 107-116 neuropeptide Y Homo sapiens 98-101 18037403-15 2008 Carbachol-induced phosphorylation of CPI-17 was also reduced in colonic segments from TNBS-treated rats. Carbachol 0-9 protein phosphatase 1, regulatory (inhibitor) subunit 14A Rattus norvegicus 37-43 17920706-4 2008 In endothelin-1 or carbachol-contracted mucosal muscle strips, CNP caused moderate, sustained and concentration-dependent relaxation. Carbachol 19-28 natriuretic peptide C Homo sapiens 63-66 18061571-0 2008 Transcriptional response to muscarinic acetylcholine receptor stimulation: regulation of Egr-1 biosynthesis by ERK, Elk-1, MKP-1, and calcineurin in carbachol-stimulated human neuroblastoma cells. Carbachol 149-158 early growth response 1 Homo sapiens 89-94 18061571-0 2008 Transcriptional response to muscarinic acetylcholine receptor stimulation: regulation of Egr-1 biosynthesis by ERK, Elk-1, MKP-1, and calcineurin in carbachol-stimulated human neuroblastoma cells. Carbachol 149-158 mitogen-activated protein kinase 1 Homo sapiens 111-114 18061571-0 2008 Transcriptional response to muscarinic acetylcholine receptor stimulation: regulation of Egr-1 biosynthesis by ERK, Elk-1, MKP-1, and calcineurin in carbachol-stimulated human neuroblastoma cells. Carbachol 149-158 ETS transcription factor ELK1 Homo sapiens 116-121 18061571-0 2008 Transcriptional response to muscarinic acetylcholine receptor stimulation: regulation of Egr-1 biosynthesis by ERK, Elk-1, MKP-1, and calcineurin in carbachol-stimulated human neuroblastoma cells. Carbachol 149-158 dual specificity phosphatase 1 Homo sapiens 123-128 18061571-1 2008 Carbachol-mediated activation of type M(3) muscarinic acetylcholine receptors induces the biosynthesis of the transcription factor Egr-1 in human SH-SY5Y neuroblastoma cells involving an activation of extracellular signal-regulated protein kinase. Carbachol 0-9 early growth response 1 Homo sapiens 131-136 18061571-2 2008 Carbachol triggered the phosphorylation of the ternary complex factor Elk-1, a key transcriptional regulator of serum response element-driven gene transcription, and strikingly enhanced the transcriptional activation potential of Elk-1. Carbachol 0-9 ETS transcription factor ELK1 Homo sapiens 70-75 18061571-2 2008 Carbachol triggered the phosphorylation of the ternary complex factor Elk-1, a key transcriptional regulator of serum response element-driven gene transcription, and strikingly enhanced the transcriptional activation potential of Elk-1. Carbachol 0-9 ETS transcription factor ELK1 Homo sapiens 230-235 18061571-5 2008 Lentiviral-mediated expression of either MAP kinase phosphatase-1 (MKP-1) or a constitutively active mutant of calcineurin A inhibited Egr-1 biosynthesis following carbachol stimulation, indicating that these phosphatases function as shut-off devices of muscarinic acetylcholine receptor signaling. Carbachol 164-173 dual specificity phosphatase 1 Homo sapiens 41-65 18061571-5 2008 Lentiviral-mediated expression of either MAP kinase phosphatase-1 (MKP-1) or a constitutively active mutant of calcineurin A inhibited Egr-1 biosynthesis following carbachol stimulation, indicating that these phosphatases function as shut-off devices of muscarinic acetylcholine receptor signaling. Carbachol 164-173 dual specificity phosphatase 1 Homo sapiens 67-72 18061571-5 2008 Lentiviral-mediated expression of either MAP kinase phosphatase-1 (MKP-1) or a constitutively active mutant of calcineurin A inhibited Egr-1 biosynthesis following carbachol stimulation, indicating that these phosphatases function as shut-off devices of muscarinic acetylcholine receptor signaling. Carbachol 164-173 early growth response 1 Homo sapiens 135-140 18061571-7 2008 Expression experiments revealed that both MKP-1 and a constitutively active mutant of calcineurin A impaired carbachol-induced upregulation of AP-1 activity. Carbachol 109-118 dual specificity phosphatase 1 Homo sapiens 42-47 18061571-8 2008 The fact that carbachol stimulation of neuroblastoma cells activates the transcription factors Egr-1 and AP-1 suggests that changes in the gene expression pattern are an integral part of muscarinic acetylcholine receptor signaling. Carbachol 14-23 early growth response 1 Homo sapiens 95-100 18214744-2 2008 MATERIALS AND METHODS: Obestatin (10(-5) M) or ghrelin (10(-5) M) were tested on two consecutive carbachol-or epinephrine-elicited contractions of iris rabbit sphincter or dilator muscles. Carbachol 97-106 LOC100101616 Oryctolagus cuniculus 47-54 18214744-4 2008 RESULTS: Compared with the first, tension of the second carbachol-induced contraction of the iris sphincter decreased 11.5+/-5.5% in the vehicle group, increased 19.0+/-10.2% in presence of obestatin, and remained unchanged by ghrelin. Carbachol 56-65 LOC100101616 Oryctolagus cuniculus 227-234 17582410-6 2008 In isolated intestinal bulb and mid/distal intestine preparations, ghrelin, motilin, and the motilin receptor agonist erythromycin all evoked contraction; these responses ranged between 9% and 51% of the contractions evoked by carbachol (10(-6) M). Carbachol 227-236 motilin Rattus norvegicus 76-83 17582410-6 2008 In isolated intestinal bulb and mid/distal intestine preparations, ghrelin, motilin, and the motilin receptor agonist erythromycin all evoked contraction; these responses ranged between 9% and 51% of the contractions evoked by carbachol (10(-6) M). Carbachol 227-236 motilin Rattus norvegicus 93-100 18514752-3 2008 Using our pharmacodynamic model to assess ex vivo AHR isolated murine tracheal rings, we found that TNFalpha-induced enhanced contractile responses to carbachol and bradykinin was abrogated by neutralizing anti-IFNbeta antibody or in tracheal rings deficient in CD38. Carbachol 151-160 tumor necrosis factor Mus musculus 100-108 18514752-3 2008 Using our pharmacodynamic model to assess ex vivo AHR isolated murine tracheal rings, we found that TNFalpha-induced enhanced contractile responses to carbachol and bradykinin was abrogated by neutralizing anti-IFNbeta antibody or in tracheal rings deficient in CD38. Carbachol 151-160 interferon beta 1, fibroblast Mus musculus 211-218 18514752-3 2008 Using our pharmacodynamic model to assess ex vivo AHR isolated murine tracheal rings, we found that TNFalpha-induced enhanced contractile responses to carbachol and bradykinin was abrogated by neutralizing anti-IFNbeta antibody or in tracheal rings deficient in CD38. Carbachol 151-160 CD38 antigen Mus musculus 262-266 18514752-4 2008 In cultured human ASM cells, where CD38 has been involved in TNFalpha-induced enhanced calcium signals to carbachol and bradykinin, we found that neutralizing anti-IFNbeta prevented TNFalpha enhancing action only on carbachol responses but not to that induced by bradykinin. Carbachol 106-115 CD38 molecule Homo sapiens 35-39 18514752-4 2008 In cultured human ASM cells, where CD38 has been involved in TNFalpha-induced enhanced calcium signals to carbachol and bradykinin, we found that neutralizing anti-IFNbeta prevented TNFalpha enhancing action only on carbachol responses but not to that induced by bradykinin. Carbachol 106-115 tumor necrosis factor Homo sapiens 61-69 18514752-4 2008 In cultured human ASM cells, where CD38 has been involved in TNFalpha-induced enhanced calcium signals to carbachol and bradykinin, we found that neutralizing anti-IFNbeta prevented TNFalpha enhancing action only on carbachol responses but not to that induced by bradykinin. Carbachol 106-115 interferon beta 1, fibroblast Mus musculus 164-171 18514752-4 2008 In cultured human ASM cells, where CD38 has been involved in TNFalpha-induced enhanced calcium signals to carbachol and bradykinin, we found that neutralizing anti-IFNbeta prevented TNFalpha enhancing action only on carbachol responses but not to that induced by bradykinin. Carbachol 106-115 tumor necrosis factor Homo sapiens 182-190 18514752-4 2008 In cultured human ASM cells, where CD38 has been involved in TNFalpha-induced enhanced calcium signals to carbachol and bradykinin, we found that neutralizing anti-IFNbeta prevented TNFalpha enhancing action only on carbachol responses but not to that induced by bradykinin. Carbachol 216-225 tumor necrosis factor Homo sapiens 61-69 18514752-4 2008 In cultured human ASM cells, where CD38 has been involved in TNFalpha-induced enhanced calcium signals to carbachol and bradykinin, we found that neutralizing anti-IFNbeta prevented TNFalpha enhancing action only on carbachol responses but not to that induced by bradykinin. Carbachol 216-225 interferon beta 1, fibroblast Mus musculus 164-171 18514752-4 2008 In cultured human ASM cells, where CD38 has been involved in TNFalpha-induced enhanced calcium signals to carbachol and bradykinin, we found that neutralizing anti-IFNbeta prevented TNFalpha enhancing action only on carbachol responses but not to that induced by bradykinin. Carbachol 216-225 tumor necrosis factor Homo sapiens 182-190 17540859-6 2008 In contrast, carbachol-induced internalization of mutant hM(1) receptors possessing either C259A/C260A or C262A/C263A or both double point mutations was significantly reduced compared to the wild-type hM(1) receptor. Carbachol 13-22 cholinergic receptor muscarinic 1 Homo sapiens 57-62 17540859-6 2008 In contrast, carbachol-induced internalization of mutant hM(1) receptors possessing either C259A/C260A or C262A/C263A or both double point mutations was significantly reduced compared to the wild-type hM(1) receptor. Carbachol 13-22 cholinergic receptor muscarinic 1 Homo sapiens 201-206 17540859-7 2008 Of the hM(1) receptor mutants tested, those possessing a C262D/C263D double point mutation had the least carbachol-induced internalization. Carbachol 105-114 cholinergic receptor muscarinic 1 Homo sapiens 7-12 17689570-8 2008 IPSPs that are increased by carbachol (CCh-sIPSPs), are depressed by CCK, omega-conotoxin GVIA, and endocannabinoids. Carbachol 28-37 cholecystokinin Homo sapiens 69-72 17997396-4 2007 The influence of carbachol, a non-specific cholinergic agonist, on locomotor activity, c-fos expression in the SCN, and the activity of this structure has been previously studied. Carbachol 17-26 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 87-92 17904551-13 2007 Endocytosed PRL was stored in intact form and released in response to stimulation with carbachol. Carbachol 87-96 prolactin Oryctolagus cuniculus 12-15 17919561-10 2007 Carbachol-stimulated MAPK activity was inhibited by three PKC inhibitors, calphostin C, chelethyrine, and staurosporine. Carbachol 0-9 mitogen activated protein kinase 3 Rattus norvegicus 21-25 17618452-9 2007 Carbachol (100 microM) and forskolin (5 microM) stimulated gastric acid secretion to a similar extent in sgk1 (-/-) and sgk1 (+/+)mice. Carbachol 0-9 serum/glucocorticoid regulated kinase 1 Mus musculus 105-109 17618452-9 2007 Carbachol (100 microM) and forskolin (5 microM) stimulated gastric acid secretion to a similar extent in sgk1 (-/-) and sgk1 (+/+)mice. Carbachol 0-9 serum/glucocorticoid regulated kinase 1 Mus musculus 120-124 17687004-10 2007 DBcAMP and VIP inhibited carbachol- and EGF-stimulated MAPK activity. Carbachol 25-34 vasoactive intestinal peptide Rattus norvegicus 11-14 17687004-10 2007 DBcAMP and VIP inhibited carbachol- and EGF-stimulated MAPK activity. Carbachol 25-34 mitogen activated protein kinase 3 Rattus norvegicus 55-59 17996892-5 2008 NPY reduced the heart rate response to vagal stimulation at 1, 3 and 5 Hz (significant at 100 nM and reaching a plateau at 250 nM NPY, p<0.05, n=6) but not to the stable analogue of acetylcholine, carbamylcholine (30, 60 or 90 nM, n=6) which produced similar degrees of bradycardia. Carbachol 200-215 pro-neuropeptide Y Cavia porcellus 0-3 17998138-11 2008 Even though adenosine and CPA alone had only a moderate effect on secretion, the observed synergistic effect with carbachol suggests a modulatory role for the adenosine A1 receptor in the lacrimal gland. Carbachol 114-123 adenosine receptor A1 Oryctolagus cuniculus 159-180 18306282-6 2008 We showed that carbachol activation of the MS-DB generated large theta oscillations in the CA1 and CA3 regions of the hippocampus. Carbachol 15-24 carbonic anhydrase 1 Rattus norvegicus 91-94 18306282-6 2008 We showed that carbachol activation of the MS-DB generated large theta oscillations in the CA1 and CA3 regions of the hippocampus. Carbachol 15-24 carbonic anhydrase 3 Rattus norvegicus 99-102 18184179-2 2008 METHODS: We have evaluated the effects of M-1 on the contractions induced by carbachol, KCl, CaCl(2), and electrical field stimulation (EFS) in human detrusor smooth muscles, and pelvic nerve stimulation-induced bladder contractions in rats. Carbachol 77-86 myoregulin Homo sapiens 42-45 18184179-5 2008 M-1 caused concentration-dependent reduction in the maximum contractile responses induced by carbachol. Carbachol 93-102 myoregulin Homo sapiens 0-3 17928569-8 2008 In the presence of M-2, the CCh-induced inhibition of I(Ca) was blocked. Carbachol 28-31 cholinergic receptor, muscarinic 2, cardiac Mus musculus 19-22 17925457-2 2008 Carbachol-activated TRPC5 currents were recorded by the whole-cell patch clamp technique from human embryonic kidney 293 cells transiently transfected with TRPC5 and the M1 muscarinic receptor. Carbachol 0-9 transient receptor potential cation channel subfamily C member 5 Homo sapiens 20-25 17925457-2 2008 Carbachol-activated TRPC5 currents were recorded by the whole-cell patch clamp technique from human embryonic kidney 293 cells transiently transfected with TRPC5 and the M1 muscarinic receptor. Carbachol 0-9 transient receptor potential cation channel subfamily C member 5 Homo sapiens 156-161 18066127-0 2007 Role of calmodulin and myosin light chain kinase in the activation of carbachol-activated cationic current in murine ileal myocytes. Carbachol 70-79 calmodulin 2 Mus musculus 8-18 18066127-0 2007 Role of calmodulin and myosin light chain kinase in the activation of carbachol-activated cationic current in murine ileal myocytes. Carbachol 70-79 myosin light chain kinase 3 Mus musculus 23-48 18066127-5 2007 The amplitude of I CCh was reduced by pretreatment either with W-7, trifluoroperazine, W-5, and melittin (CaM inhibitors) or with ML-7 and ML-9 (selective MLCK inhibitors), and the inhibitory effects were reversible. Carbachol 19-22 calmodulin 2 Mus musculus 106-109 18066127-5 2007 The amplitude of I CCh was reduced by pretreatment either with W-7, trifluoroperazine, W-5, and melittin (CaM inhibitors) or with ML-7 and ML-9 (selective MLCK inhibitors), and the inhibitory effects were reversible. Carbachol 19-22 solute carrier family 25 (mitochondrial carrier, Graves disease autoantigen), member 16 Mus musculus 130-134 18066127-5 2007 The amplitude of I CCh was reduced by pretreatment either with W-7, trifluoroperazine, W-5, and melittin (CaM inhibitors) or with ML-7 and ML-9 (selective MLCK inhibitors), and the inhibitory effects were reversible. Carbachol 19-22 myosin light chain kinase 3 Mus musculus 155-159 18066127-8 2007 We conclude that CaM and MLCK modulate the activation process of I CCh in murine ileal myocytes and suggest that the classical type transient receptor potential (TRPC) channel 5 might be a candidate for nonselective cationic currents (NSCC) activated by muscarinic stimulation in gastrointestinal smooth muscle cells. Carbachol 67-70 calmodulin 2 Mus musculus 17-20 18066127-8 2007 We conclude that CaM and MLCK modulate the activation process of I CCh in murine ileal myocytes and suggest that the classical type transient receptor potential (TRPC) channel 5 might be a candidate for nonselective cationic currents (NSCC) activated by muscarinic stimulation in gastrointestinal smooth muscle cells. Carbachol 67-70 myosin light chain kinase 3 Mus musculus 25-29 17394068-4 2007 In the perfused esophagus, addition of carbachol increased mucin secretion by approximately 2-fold. Carbachol 39-48 LOC100508689 Homo sapiens 59-64 17920045-4 2007 Carbachol injected into the IGL of hamsters in their subjective day (CT8) induced phase advances similar to shifts that are induced by arousal at the same circadian time. Carbachol 0-9 leucine zipper protein 4 Homo sapiens 69-72 17707768-3 2007 Zymography of N18TG2 culture medium revealed no gelatinolytic activity, whereas after carbachol treatment of cells both MMP-9 and activated MMP-2 forms were detected. Carbachol 86-95 matrix metallopeptidase 9 Mus musculus 120-125 17707768-3 2007 Zymography of N18TG2 culture medium revealed no gelatinolytic activity, whereas after carbachol treatment of cells both MMP-9 and activated MMP-2 forms were detected. Carbachol 86-95 matrix metallopeptidase 2 Mus musculus 140-145 17707768-5 2007 Carbachol treatment increased MMP-2 and MMP-9 gene expression in N18TG2 cells and higher levels for both genes were also observed in ChAT transfected cells. Carbachol 0-9 matrix metallopeptidase 2 Mus musculus 30-35 17707768-5 2007 Carbachol treatment increased MMP-2 and MMP-9 gene expression in N18TG2 cells and higher levels for both genes were also observed in ChAT transfected cells. Carbachol 0-9 matrix metallopeptidase 9 Mus musculus 40-45 17644755-3 2007 The isometric tension of tracheal rings from CAT-2-/- mice showed a significant decrease in carbachol (CCh)-induced force generation (33.01%, P < 0.05, n = 8) compared with controls. Carbachol 92-101 dominant cataract 2 Mus musculus 45-50 17964734-4 2007 In region CA1 of the rat (Sprague Dawley) hippocampus, the cholinergic agonist carbachol (CCh) suppresses transmission in stratum radiatum (SR), at synapses of the Schaffer collateral projection from CA3, while having lesser effects in stratum lacunosum-moleculare (SLM), the perforant path projection from entorhinal cortex (Hasselmo and Schnell, 1994). Carbachol 79-88 carbonic anhydrase 1 Rattus norvegicus 10-13 17964734-4 2007 In region CA1 of the rat (Sprague Dawley) hippocampus, the cholinergic agonist carbachol (CCh) suppresses transmission in stratum radiatum (SR), at synapses of the Schaffer collateral projection from CA3, while having lesser effects in stratum lacunosum-moleculare (SLM), the perforant path projection from entorhinal cortex (Hasselmo and Schnell, 1994). Carbachol 79-88 carbonic anhydrase 3 Rattus norvegicus 200-203 17964734-4 2007 In region CA1 of the rat (Sprague Dawley) hippocampus, the cholinergic agonist carbachol (CCh) suppresses transmission in stratum radiatum (SR), at synapses of the Schaffer collateral projection from CA3, while having lesser effects in stratum lacunosum-moleculare (SLM), the perforant path projection from entorhinal cortex (Hasselmo and Schnell, 1994). Carbachol 90-93 carbonic anhydrase 1 Rattus norvegicus 10-13 17964734-4 2007 In region CA1 of the rat (Sprague Dawley) hippocampus, the cholinergic agonist carbachol (CCh) suppresses transmission in stratum radiatum (SR), at synapses of the Schaffer collateral projection from CA3, while having lesser effects in stratum lacunosum-moleculare (SLM), the perforant path projection from entorhinal cortex (Hasselmo and Schnell, 1994). Carbachol 90-93 carbonic anhydrase 3 Rattus norvegicus 200-203 17644755-3 2007 The isometric tension of tracheal rings from CAT-2-/- mice showed a significant decrease in carbachol (CCh)-induced force generation (33.01%, P < 0.05, n = 8) compared with controls. Carbachol 103-106 dominant cataract 2 Mus musculus 45-50 17880368-7 2007 Contractile responses to muscarinic agonists (carbachol or AHR-602) and high K(+) were desensitized by pretreatment with 10(-6) mol/L carbachol for 30 min in a manner dependent on the presence of extracellular Ca(2+). Carbachol 134-143 aryl hydrocarbon receptor Cavia porcellus 59-62 17442131-8 2007 Immunohistochemistry for proliferating cell nuclear antigen (PCNA) showed that the 3-day Cch infusion significantly increased the number of PCNA-immunoreactive cells in the liver. Carbachol 89-92 proliferating cell nuclear antigen Rattus norvegicus 25-59 17984593-9 2007 The suppression of the carbachol-induced contraction was completely restored by COX inhibitor, indomethacin. Carbachol 23-32 coproporphyrinogen oxidase Rattus norvegicus 80-83 17320950-10 2007 PMA also reduced the Ca2+ response of intact RINm5F cells stimulated with carbachol and EGF, two agonists that use different receptor types to activate phospholipase C. These results suggest the existence of a negative feedback mechanism involving two components of the Ca2+ signalling cascade, whereby activated PKC dampens IP3R-3 activity. Carbachol 74-83 protein kinase C, gamma Rattus norvegicus 313-316 17320950-10 2007 PMA also reduced the Ca2+ response of intact RINm5F cells stimulated with carbachol and EGF, two agonists that use different receptor types to activate phospholipase C. These results suggest the existence of a negative feedback mechanism involving two components of the Ca2+ signalling cascade, whereby activated PKC dampens IP3R-3 activity. Carbachol 74-83 inositol 1,4,5-trisphosphate receptor, type 3 Rattus norvegicus 325-331 17560078-6 2007 The increased efficacy of iG(q)alpha compared to mG(q)alpha was also seen downstream of PLC with carbachol stimulation of the mitogen-activated protein kinase, ERK1/2. Carbachol 97-106 heparan sulfate proteoglycan 2 Homo sapiens 88-91 17560078-6 2007 The increased efficacy of iG(q)alpha compared to mG(q)alpha was also seen downstream of PLC with carbachol stimulation of the mitogen-activated protein kinase, ERK1/2. Carbachol 97-106 mitogen-activated protein kinase 3 Homo sapiens 160-166 17928641-6 2007 pY-ERK level was strongly elevated by 10 nM CCK-8, 100 microM carbachol (CAR), or 100 nM EGF. Carbachol 62-71 mitogen-activated protein kinase 1 Homo sapiens 3-6 17928641-6 2007 pY-ERK level was strongly elevated by 10 nM CCK-8, 100 microM carbachol (CAR), or 100 nM EGF. Carbachol 73-76 mitogen-activated protein kinase 1 Homo sapiens 3-6 17688889-7 2007 Endothelin-1 and norepinephrine also increased the CCh (10 microM)-induced [Ca2+]i elevation. Carbachol 51-54 endothelin 1 Homo sapiens 0-12 17803048-0 2007 Carbamoylcholine chloride induces a rapid increase in IL6 in the nasal cavity of C57BL/6 mice. Carbachol 0-25 interleukin 6 Mus musculus 54-57 17803048-2 2007 In our work to determine the basal levels of cytokines in saliva, nasal wash fluid (NWF), bronchoalveolar lavage fluid (BALF), and serum of mice, we found that injection of carbamoylcholine chloride, used to stimulate saliva production, induced variations in the interleukin (IL) 6 levels of NWF and BALF supernatants. Carbachol 173-198 interleukin 6 Mus musculus 263-281 17803048-5 2007 To determine whether carbamoylcholine chloride increased Th1 cytokine (IL2, IL12[p70], and interferon gamma), Th2 cytokine (IL4, IL5, and IL10), granulocyte-macrophage colony-stimulating factor (GM-CSF), or proinflammatory cytokine (IL1beta, tumor necrosis factor alpha, and IL6 in saliva and serum) levels, mice were given 10 microg carbamoylcholine chloride and euthanized. Carbachol 21-46 negative elongation factor complex member C/D, Th1l Mus musculus 57-60 17803048-5 2007 To determine whether carbamoylcholine chloride increased Th1 cytokine (IL2, IL12[p70], and interferon gamma), Th2 cytokine (IL4, IL5, and IL10), granulocyte-macrophage colony-stimulating factor (GM-CSF), or proinflammatory cytokine (IL1beta, tumor necrosis factor alpha, and IL6 in saliva and serum) levels, mice were given 10 microg carbamoylcholine chloride and euthanized. Carbachol 21-46 interleukin 2 Mus musculus 71-74 17803048-5 2007 To determine whether carbamoylcholine chloride increased Th1 cytokine (IL2, IL12[p70], and interferon gamma), Th2 cytokine (IL4, IL5, and IL10), granulocyte-macrophage colony-stimulating factor (GM-CSF), or proinflammatory cytokine (IL1beta, tumor necrosis factor alpha, and IL6 in saliva and serum) levels, mice were given 10 microg carbamoylcholine chloride and euthanized. Carbachol 21-46 interferon gamma Mus musculus 91-107 17803048-5 2007 To determine whether carbamoylcholine chloride increased Th1 cytokine (IL2, IL12[p70], and interferon gamma), Th2 cytokine (IL4, IL5, and IL10), granulocyte-macrophage colony-stimulating factor (GM-CSF), or proinflammatory cytokine (IL1beta, tumor necrosis factor alpha, and IL6 in saliva and serum) levels, mice were given 10 microg carbamoylcholine chloride and euthanized. Carbachol 21-46 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 195-201 17803048-5 2007 To determine whether carbamoylcholine chloride increased Th1 cytokine (IL2, IL12[p70], and interferon gamma), Th2 cytokine (IL4, IL5, and IL10), granulocyte-macrophage colony-stimulating factor (GM-CSF), or proinflammatory cytokine (IL1beta, tumor necrosis factor alpha, and IL6 in saliva and serum) levels, mice were given 10 microg carbamoylcholine chloride and euthanized. Carbachol 21-46 interleukin 1 beta Mus musculus 233-240 17803048-5 2007 To determine whether carbamoylcholine chloride increased Th1 cytokine (IL2, IL12[p70], and interferon gamma), Th2 cytokine (IL4, IL5, and IL10), granulocyte-macrophage colony-stimulating factor (GM-CSF), or proinflammatory cytokine (IL1beta, tumor necrosis factor alpha, and IL6 in saliva and serum) levels, mice were given 10 microg carbamoylcholine chloride and euthanized. Carbachol 21-46 tumor necrosis factor Mus musculus 242-269 17803048-5 2007 To determine whether carbamoylcholine chloride increased Th1 cytokine (IL2, IL12[p70], and interferon gamma), Th2 cytokine (IL4, IL5, and IL10), granulocyte-macrophage colony-stimulating factor (GM-CSF), or proinflammatory cytokine (IL1beta, tumor necrosis factor alpha, and IL6 in saliva and serum) levels, mice were given 10 microg carbamoylcholine chloride and euthanized. Carbachol 21-46 interleukin 6 Mus musculus 275-278 17803048-7 2007 In summary, carbamoylcholine chloride induces a rapid, elevated IL6 response in the nasal cavity and respiratory tract of mice but does not alter the levels of other Th1, Th2, or proinflammatory cytokines. Carbachol 12-37 interleukin 6 Mus musculus 64-67 17652747-8 2007 Parasympathomimetic stimulation by carbachol stimulated NHE and AE through the elevation of intracellular calcium concentration. Carbachol 35-44 solute carrier family 9 member C1 Homo sapiens 56-59 17675796-6 2007 Furthermore, the AChR agonists nicotine and carbachol did not affect the neurite outgrowth induced by NGF. Carbachol 44-53 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 17-21 17395899-11 2007 Incubation of colon tissue with carbamylcholine or deoxycholate to stimulate exocytosis by goblet cells caused a partial redistribution of Rab3D to the cytoplasm and mucous granule field and a concomitant transformation of the Golgi architecture. Carbachol 32-47 RAB3D, member RAS oncogene family Rattus norvegicus 139-144 17611397-9 2007 NOS1 and NOS3 isoforms are expressed in MCF-7 cells and its activation by CARB triggers nitric oxide synthesis and vascular endothelial growth factor expression increasing blood vessels formation induced by mammary tumor cells in vivo. Carbachol 74-78 nitric oxide synthase 1 Homo sapiens 0-4 17611397-9 2007 NOS1 and NOS3 isoforms are expressed in MCF-7 cells and its activation by CARB triggers nitric oxide synthesis and vascular endothelial growth factor expression increasing blood vessels formation induced by mammary tumor cells in vivo. Carbachol 74-78 nitric oxide synthase 3 Homo sapiens 9-13 17611397-9 2007 NOS1 and NOS3 isoforms are expressed in MCF-7 cells and its activation by CARB triggers nitric oxide synthesis and vascular endothelial growth factor expression increasing blood vessels formation induced by mammary tumor cells in vivo. Carbachol 74-78 vascular endothelial growth factor A Homo sapiens 115-149 17644755-8 2007 The reduced airway smooth muscle (ASM) contractility to CCh seen in the CAT-2-/- tracheal rings was completely reversed by pretreating the rings with 100 muM spermine. Carbachol 56-59 dominant cataract 2 Mus musculus 72-77 17643958-1 2007 We previously showed that stimulation of muscarinic acetylcholine receptors (mAChR) by carbachol (Cch) caused a time- and dose-dependent increase of mitogen-activated protein kinase/extracellular signal-regulated kinases (MAPK/ERK) phosphorylation in thyroid epithelial cells. Carbachol 87-96 mitogen-activated protein kinase 1 Homo sapiens 227-230 17643958-1 2007 We previously showed that stimulation of muscarinic acetylcholine receptors (mAChR) by carbachol (Cch) caused a time- and dose-dependent increase of mitogen-activated protein kinase/extracellular signal-regulated kinases (MAPK/ERK) phosphorylation in thyroid epithelial cells. Carbachol 98-101 mitogen-activated protein kinase 1 Homo sapiens 227-230 17643958-4 2007 Incorporation of Pyk2 antisense oligonucleotides in thyroid epithelial cells to down-regulated Pyk2 expression or pretreatment of cells with the Ca(2+)/calmodulin protein kinase II (CaM kinase II) inhibitor KN-62 significantly reduced Cch-induced MAPK/ERK phosphorylation. Carbachol 235-238 protein tyrosine kinase 2 beta Homo sapiens 17-21 17643958-5 2007 In addition, Cch-induced MAPK/ERK phosphorylation was partially inhibited by LY294002 and wortmannin, two selective inhibitors of phosphatidylinositol 3-kinase (PI3K), tyrphostin AG1478, a specific inhibitor of epidermal growth factor receptor (EGFR) kinase, and (-)-perillic acid, a post-translational inhibitor of small G-proteins isoprenylation. Carbachol 13-16 mitogen-activated protein kinase 1 Homo sapiens 30-33 17643958-5 2007 In addition, Cch-induced MAPK/ERK phosphorylation was partially inhibited by LY294002 and wortmannin, two selective inhibitors of phosphatidylinositol 3-kinase (PI3K), tyrphostin AG1478, a specific inhibitor of epidermal growth factor receptor (EGFR) kinase, and (-)-perillic acid, a post-translational inhibitor of small G-proteins isoprenylation. Carbachol 13-16 epidermal growth factor receptor Homo sapiens 211-243 17643958-5 2007 In addition, Cch-induced MAPK/ERK phosphorylation was partially inhibited by LY294002 and wortmannin, two selective inhibitors of phosphatidylinositol 3-kinase (PI3K), tyrphostin AG1478, a specific inhibitor of epidermal growth factor receptor (EGFR) kinase, and (-)-perillic acid, a post-translational inhibitor of small G-proteins isoprenylation. Carbachol 13-16 epidermal growth factor receptor Homo sapiens 245-249 17669399-10 2007 Activation of ET-BR by IRL-1620 (5 x 10(-7)M) results in contraction of native BTM (41% of the carbachol value) and also in a transient increase in [Ca(2+)](i) in cultured BTM and HTM cells (365 and 273% of the basal level, respectively). Carbachol 95-104 endothelin receptor type B Bos taurus 14-19 17923193-9 2007 RESULTS: The peak levels of force development induced by carbachol were 139.1% +/- 5.0% in EDNRB(-/-) rat, whereas the peak levels were 242.1% +/- 27.7% in EDNRB(+/+) rat. Carbachol 57-66 endothelin receptor type B Rattus norvegicus 91-96 17603544-4 2007 KEY RESULTS: Cationic currents elicited by carbachol (CCh; 100 microM) in HEK293 cells overexpressing murine TRPC6 (I(TRPC6)) were dose-dependently inhibited by externally applied ML-9 (IC(50)=7.8 microM). Carbachol 43-52 transient receptor potential cation channel, subfamily C, member 6 Mus musculus 109-114 17603544-4 2007 KEY RESULTS: Cationic currents elicited by carbachol (CCh; 100 microM) in HEK293 cells overexpressing murine TRPC6 (I(TRPC6)) were dose-dependently inhibited by externally applied ML-9 (IC(50)=7.8 microM). Carbachol 43-52 transient receptor potential cation channel, subfamily C, member 6 Mus musculus 118-123 17603544-4 2007 KEY RESULTS: Cationic currents elicited by carbachol (CCh; 100 microM) in HEK293 cells overexpressing murine TRPC6 (I(TRPC6)) were dose-dependently inhibited by externally applied ML-9 (IC(50)=7.8 microM). Carbachol 54-57 transient receptor potential cation channel, subfamily C, member 6 Mus musculus 109-114 17603544-4 2007 KEY RESULTS: Cationic currents elicited by carbachol (CCh; 100 microM) in HEK293 cells overexpressing murine TRPC6 (I(TRPC6)) were dose-dependently inhibited by externally applied ML-9 (IC(50)=7.8 microM). Carbachol 54-57 transient receptor potential cation channel, subfamily C, member 6 Mus musculus 118-123 17569886-4 2007 In contrast, when CD34+ cells are cultured in EGM-2 supplemented with platelet-derived growth factor-BB (50 ng/mL), they give rise to SM-like cells characterized by spindle-shape morphology, expression of SM cell markers (alpha-SM actin, SM myosin heavy chain, calponin, caldesmon, SM alpha-22), and the ability to contract and relax in response to common pharmacological agents such as carbachol and atropine but rarely form capillary-like structures when placed in Matrigel. Carbachol 387-396 CD34 molecule Homo sapiens 18-22 17573185-9 2007 There was an increase in SNARE complex formation in islets stimulated with prolactin, 22 mM glucose, 40 mM K(+), 200 microM carbachol and 1 microM PMA. Carbachol 124-133 prolactin Rattus norvegicus 75-84 17373911-8 2007 The PKA-induced enhancement of IP(3)R-3 activity was also observed in intact RINm5F cells stimulated with carbachol and epidermal growth factor, two agonists that use different receptor types to activate phospholipase C. CONCLUSION: The results of the present study reveal a converging step where the cAMP and the Ca2+ signalling systems act co-operatively in endocrine cell responses to external stimuli. Carbachol 106-115 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 4-7 17373911-8 2007 The PKA-induced enhancement of IP(3)R-3 activity was also observed in intact RINm5F cells stimulated with carbachol and epidermal growth factor, two agonists that use different receptor types to activate phospholipase C. CONCLUSION: The results of the present study reveal a converging step where the cAMP and the Ca2+ signalling systems act co-operatively in endocrine cell responses to external stimuli. Carbachol 106-115 inositol 1,4,5-trisphosphate receptor, type 3 Rattus norvegicus 31-39 17591863-10 2007 Carbachol also activated p42/44 MAPK in a time-dependent manner. Carbachol 0-9 cyclin dependent kinase 20 Homo sapiens 25-28 17591863-11 2007 Preincubation with U0126 abolished carbachol-induced p42/44 MAPK activation and cell proliferation. Carbachol 35-44 cyclin dependent kinase 20 Homo sapiens 53-56 17395173-1 2007 Carbachol-induced detrusor contractions are mainly mediated via M3 receptor subtype and depend not only on Ca2+ release from the intracellular calcium stores but also on Ca2+ influx via L-type Ca2+ channels. Carbachol 0-9 cholinergic receptor muscarinic 3 Homo sapiens 64-66 17395173-6 2007 Carbachol-induced release of intracellular Ca2+ in Chinese hamster ovary cells expressing muscarinic hM3 receptors was completely prevented by 100 microM 2-APB. Carbachol 0-9 cholinergic receptor muscarinic 3 Homo sapiens 101-104 17395173-7 2007 The direct intracellular IP3 receptor antagonist xestospongin C (10 microM) reduced carbachol-stimulated intracellular Ca2+ to 41% of the control value. Carbachol 84-93 inositol 1,4,5-trisphosphate receptor type 3 Homo sapiens 25-37 17307332-4 2007 Similarly, PKC inhibition enhanced EGFR transactivation in human colonic epithelial T84 cells stimulated with carbachol, as well as in bombesin-stimulated Rat-1 fibroblasts stably transfected with the bombesin receptor. Carbachol 110-119 protein kinase C, alpha Rattus norvegicus 11-14 17307332-4 2007 Similarly, PKC inhibition enhanced EGFR transactivation in human colonic epithelial T84 cells stimulated with carbachol, as well as in bombesin-stimulated Rat-1 fibroblasts stably transfected with the bombesin receptor. Carbachol 110-119 epidermal growth factor receptor Homo sapiens 35-39 17442131-8 2007 Immunohistochemistry for proliferating cell nuclear antigen (PCNA) showed that the 3-day Cch infusion significantly increased the number of PCNA-immunoreactive cells in the liver. Carbachol 89-92 proliferating cell nuclear antigen Rattus norvegicus 61-65 17442131-8 2007 Immunohistochemistry for proliferating cell nuclear antigen (PCNA) showed that the 3-day Cch infusion significantly increased the number of PCNA-immunoreactive cells in the liver. Carbachol 89-92 proliferating cell nuclear antigen Rattus norvegicus 140-144 17442131-9 2007 Moreover, the sera from the Cch-infused rats increased the number of PCNA-immunoreactive hepatocytes in culture. Carbachol 28-31 proliferating cell nuclear antigen Rattus norvegicus 69-73 17442131-11 2007 When compared with the monoculture of hepatocytes, the coculture of those with hepatic NPCs resulted in enhanced PCNA immunoreactivity after a 4-day treatment with 3 mM Cch. Carbachol 169-172 proliferating cell nuclear antigen Rattus norvegicus 113-117 17637176-6 2007 The H1R level was also up-regulated by M3R activation (150% of control by treatment with carbachol for 24 h). Carbachol 89-98 histamine receptor H1 Homo sapiens 4-7 17564632-8 2007 These results suggest that the suppression of carbachol-induced contraction in mice with colitis is attributable at least partially to the increased activity of myosin phosphatase following the downregulation of CPI-17. Carbachol 46-55 protein phosphatase 1, regulatory inhibitor subunit 14A Mus musculus 212-218 17383686-4 2007 As a measure of cell proliferation ((3)H)-thymidine incorporation in primary human lung fibroblasts and MRC-5 fibroblasts was increased by about 2 fold in presence of the muscarinic receptor agonist carbachol (10 microM) and this effect could be prevented by the MEK inhibitor PD 98059 (30 microM). Carbachol 199-208 mitogen-activated protein kinase kinase 7 Homo sapiens 263-266 17383686-5 2007 Western blot analysis revealed a rapid (within 2 min) activation of p42/44 MAPK (ERK1, ERK2) following exposure to 10 microM carbachol or oxotremorine, effects blocked by tiotropium as well as atropine. Carbachol 125-134 mitogen-activated protein kinase 1 Homo sapiens 68-79 17383686-5 2007 Western blot analysis revealed a rapid (within 2 min) activation of p42/44 MAPK (ERK1, ERK2) following exposure to 10 microM carbachol or oxotremorine, effects blocked by tiotropium as well as atropine. Carbachol 125-134 mitogen-activated protein kinase 3 Homo sapiens 81-85 17383686-5 2007 Western blot analysis revealed a rapid (within 2 min) activation of p42/44 MAPK (ERK1, ERK2) following exposure to 10 microM carbachol or oxotremorine, effects blocked by tiotropium as well as atropine. Carbachol 125-134 mitogen-activated protein kinase 1 Homo sapiens 87-91 17277016-12 2007 The severe carbachol-induced sinus bradycardia in Galpha(i2)G184S mice suggests a possible role for alterations of Galpha(i2) or RGS proteins in sick sinus syndrome and pathological AV block. Carbachol 11-20 guanine nucleotide binding protein (G protein), alpha inhibiting 2 Mus musculus 50-59 17203464-8 2007 Pretreatment with the cAMP generating agent"s forskolin and vasoactive intestinal peptide (VIP) enhanced carbachol (Cch)-induced and epidermal growth factor (EGF)-induced Ca(2+) responses in intact AR4-2J cells. Carbachol 105-114 vasoactive intestinal peptide Rattus norvegicus 91-94 17203464-8 2007 Pretreatment with the cAMP generating agent"s forskolin and vasoactive intestinal peptide (VIP) enhanced carbachol (Cch)-induced and epidermal growth factor (EGF)-induced Ca(2+) responses in intact AR4-2J cells. Carbachol 116-119 vasoactive intestinal peptide Rattus norvegicus 91-94 17463038-2 2007 In cells from M2 subtype-knockout (M2-KO) or M3-KO mice, carbachol (100 microM) evoked a muscarinic cationic current (mI(Cat)) as small as approximately 10% of mI(Cat) in wild-type (WT) cells. Carbachol 57-66 catalase Mus musculus 89-125 17277016-12 2007 The severe carbachol-induced sinus bradycardia in Galpha(i2)G184S mice suggests a possible role for alterations of Galpha(i2) or RGS proteins in sick sinus syndrome and pathological AV block. Carbachol 11-20 guanine nucleotide binding protein (G protein), alpha inhibiting 2 Mus musculus 115-124 17240116-7 2007 We found that either carbachol or EGF promoted a striking ERK-dependent phosphorylation of FAK at Ser-910, but these agonists caused only slight stimulation of FAK at Tyr-397 in T84 cells. Carbachol 21-30 EPH receptor B2 Homo sapiens 58-61 17234888-6 2007 The activation of both cAMP-dependent pathways or the selective activation of Epac was found to enhance amylase secretion induced by physiological and supraphysiological concentrations of the muscarinic agonist carbachol. Carbachol 211-220 Rap guanine nucleotide exchange factor 3 Homo sapiens 78-82 17234888-7 2007 Similarly, activation of both PKA or the specific activation of Epac enhanced carbachol-induced activation of trypsinogen and chymotrypsinogen. Carbachol 78-87 Rap guanine nucleotide exchange factor 3 Homo sapiens 64-68 17240116-7 2007 We found that either carbachol or EGF promoted a striking ERK-dependent phosphorylation of FAK at Ser-910, but these agonists caused only slight stimulation of FAK at Tyr-397 in T84 cells. Carbachol 21-30 protein tyrosine kinase 2 Homo sapiens 91-94 16956963-6 2007 The responses to carbachol (CCh: 10 muM) were also greater in Pmca1(+/-) (120-150%) than in WT bladders. Carbachol 17-26 ATPase, Ca++ transporting, plasma membrane 1 Mus musculus 62-67 17384670-6 2007 These inhibitors also attenuated the carbachol induced increase in nNOS- and eNOS-mRNA levels. Carbachol 37-46 nitric oxide synthase 1 Rattus norvegicus 67-71 17384670-7 2007 Inhibition of nNOS shifted the dose response curve of carbachol on contractility to the right, whereas inhibition of eNOS shifted it to the left. Carbachol 54-63 nitric oxide synthase 1 Rattus norvegicus 14-18 17250650-2 2007 In this study, we report that agonists for G protein-coupled receptors (GPCRs), including endothelin, lysophosphatidic acid and carbachol, effectively promote the expression of SMC-specific proteins in the presence of transforming growth factor-beta (TGF-beta). Carbachol 128-137 transforming growth factor beta 1 Homo sapiens 218-249 17250650-2 2007 In this study, we report that agonists for G protein-coupled receptors (GPCRs), including endothelin, lysophosphatidic acid and carbachol, effectively promote the expression of SMC-specific proteins in the presence of transforming growth factor-beta (TGF-beta). Carbachol 128-137 transforming growth factor beta 1 Homo sapiens 251-259 17448648-1 2007 The role of endothelin, PAF and thromboxane A2 in airway hyperreactivity (AHR) to carbachol induced by ovalbumin sensitization and challenge in Balb/c mice was investigated. Carbachol 82-91 serine (or cysteine) peptidase inhibitor, clade B, member 1, pseudogene Mus musculus 103-112 17448648-2 2007 Ovalbumin sensitization and challenge induced significant AHR to carbachol in actively sensitized and challenged mice. Carbachol 65-74 serine (or cysteine) peptidase inhibitor, clade B, member 1, pseudogene Mus musculus 0-9 17448648-11 2007 ), endothelin-1 (100 pmol, intranasally) or the ET(B) receptor agonist IRL-1620 (100 pmol, intranasally) showed a marked increase in airway reactivity to carbachol. Carbachol 154-163 endothelin 1 Mus musculus 3-15 17306779-10 2007 In conclusion, results suggest that microinjection of CBH in the BST activates local M(2)-muscarinic receptor evoking pressor and bradycardiac responses, which are mediated by acute vasopressin release into circulation. Carbachol 54-57 arginine vasopressin Rattus norvegicus 182-193 17255165-2 2007 In this study, we investigated the mechanism of the receptor-mediated regulation of I(GIRK) in acutely isolated hippocampal CA1 neurons by the muscarinic receptor agonist, carbachol (CCh), and the group I metabotropic glutamate receptor (mGluR) agonist, 3,5-dihydroxyphenylglycine (DHPG). Carbachol 172-181 carbonic anhydrase 1 Rattus norvegicus 124-127 17255165-2 2007 In this study, we investigated the mechanism of the receptor-mediated regulation of I(GIRK) in acutely isolated hippocampal CA1 neurons by the muscarinic receptor agonist, carbachol (CCh), and the group I metabotropic glutamate receptor (mGluR) agonist, 3,5-dihydroxyphenylglycine (DHPG). Carbachol 183-186 carbonic anhydrase 1 Rattus norvegicus 124-127 17255165-6 2007 In PLCbeta1 knockout mice, the effect of CCh on I(GIRK) was significantly reduced, whereas the effect of DHPG remained unchanged. Carbachol 41-44 phospholipase C, beta 1 Mus musculus 3-11 17428986-6 2007 Furthermore, we establish that carbachol affects the overall phosphorylation of ADAM17 on its threonine and tyrosine but not serine residues, whereas levels of phosphorylated ADAM9 were not affected. Carbachol 31-40 ADAM metallopeptidase domain 17 Homo sapiens 80-86 17428986-8 2007 Mutations of threonine 735 but not of tyrosine 702 of the ADAM17 cytoplasmic tail abolishes the carbachol-induced increase of N1, ADAM17 phosphorylation, and JMV2770-hydrolyzing activity in M1- and M3-expressing HEK293 cells. Carbachol 96-105 ADAM metallopeptidase domain 17 Homo sapiens 58-64 17428986-8 2007 Mutations of threonine 735 but not of tyrosine 702 of the ADAM17 cytoplasmic tail abolishes the carbachol-induced increase of N1, ADAM17 phosphorylation, and JMV2770-hydrolyzing activity in M1- and M3-expressing HEK293 cells. Carbachol 96-105 ADAM metallopeptidase domain 17 Homo sapiens 130-136 17164431-6 2007 Cells secreted PRL consititutively and at increased rates in response to CCh. Carbachol 73-76 prolactin Oryctolagus cuniculus 15-18 17408632-0 2007 Alcohol-induced protein kinase Calpha phosphorylation of Munc18c in carbachol-stimulated acini causes basolateral exocytosis. Carbachol 68-77 syntaxin binding protein 3 Rattus norvegicus 57-64 17197450-6 2007 Stimulation with concentrations of glucose and carbachol that accelerate hydrolysis of endogenous AA from islet phosphoplipids also results in accelerated Kv2.1 inactivation and a shorter time-to-peak current interval. Carbachol 47-56 potassium voltage-gated channel subfamily B member 1 Rattus norvegicus 155-160 17305705-13 2007 The residual CCH effect observed in K(Ca)3.1 KO mice suggests that yet another K+ channel is driving the CCH-stimulated Cl- secretion. Carbachol 13-16 potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 Mus musculus 36-44 17305705-13 2007 The residual CCH effect observed in K(Ca)3.1 KO mice suggests that yet another K+ channel is driving the CCH-stimulated Cl- secretion. Carbachol 105-108 potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 Mus musculus 36-44 17446468-9 2007 Studies of bladder smooth muscle from PMCA4 null mutants and PMCA1 heterozygous mice suggest that PMCA1 and PMCA4 play different roles in smooth muscle contractility, with PMCA1 contributing to overall Ca2+ clearance and PMCA4 being required for carbachol-stimulated contraction. Carbachol 246-255 ATPase, Ca++ transporting, plasma membrane 1 Mus musculus 98-103 17446468-9 2007 Studies of bladder smooth muscle from PMCA4 null mutants and PMCA1 heterozygous mice suggest that PMCA1 and PMCA4 play different roles in smooth muscle contractility, with PMCA1 contributing to overall Ca2+ clearance and PMCA4 being required for carbachol-stimulated contraction. Carbachol 246-255 ATPase, Ca++ transporting, plasma membrane 4 Mus musculus 108-113 17446468-9 2007 Studies of bladder smooth muscle from PMCA4 null mutants and PMCA1 heterozygous mice suggest that PMCA1 and PMCA4 play different roles in smooth muscle contractility, with PMCA1 contributing to overall Ca2+ clearance and PMCA4 being required for carbachol-stimulated contraction. Carbachol 246-255 ATPase, Ca++ transporting, plasma membrane 1 Mus musculus 98-103 17446468-9 2007 Studies of bladder smooth muscle from PMCA4 null mutants and PMCA1 heterozygous mice suggest that PMCA1 and PMCA4 play different roles in smooth muscle contractility, with PMCA1 contributing to overall Ca2+ clearance and PMCA4 being required for carbachol-stimulated contraction. Carbachol 246-255 ATPase, Ca++ transporting, plasma membrane 4 Mus musculus 108-113 17301171-5 2007 Surprisingly, marked gender differences in GHRH neuronal activity were observed in response to the muscarinic agonist carbachol (CCh). Carbachol 118-127 growth hormone releasing hormone Mus musculus 43-47 17301171-5 2007 Surprisingly, marked gender differences in GHRH neuronal activity were observed in response to the muscarinic agonist carbachol (CCh). Carbachol 129-132 growth hormone releasing hormone Mus musculus 43-47 17301171-6 2007 In females, CCh enhanced action potential firing in all GHRH neurons. Carbachol 12-15 growth hormone releasing hormone Mus musculus 56-60 17301171-7 2007 In males, CCh enhanced action potential firing in two-thirds of GHRH neurons, whereas it decreased firing in the remainders. Carbachol 10-13 growth hormone releasing hormone Mus musculus 64-68 17307724-9 2007 In beta-escin-permeabilized ileal tissues, pretreatment with anti-phosphorylated CPI-17 antibody inhibited the carbachol-induced Ca(2+) sensitization in the presence of GTP. Carbachol 111-120 protein phosphatase 1, regulatory inhibitor subunit 14A Mus musculus 81-87 17204498-8 2007 It is important to note that glands from CFTR knockout mice responded to carbachol but did not secrete when exposed to VIP or forskolin, as has been shown previously for glands from CF patients. Carbachol 73-82 cystic fibrosis transmembrane conductance regulator Mus musculus 41-45 17305705-12 2007 CCH-stimulated DeltaIsc was significantly reduced in K(Ca)3.1 KO mice, underscoring the known relevance of this channel in the activation of Cl- secretion by an intracellular Ca2+ increasing agonist. Carbachol 0-3 potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 Mus musculus 53-61 17095754-4 2007 Although blocking the presumably apically located K(+) channel KCNQ1 with chromanol 293b reduced both the forskolin- and carbachol-induced cell shrinkage, inhibition of Ca(2+)-sensitive K(+) channels with charybdotoxin strongly inhibited the cell volume decrease after carbachol, but not after forskolin stimulation. Carbachol 121-130 potassium voltage-gated channel subfamily KQT member 1 Oryctolagus cuniculus 63-68 17095754-4 2007 Although blocking the presumably apically located K(+) channel KCNQ1 with chromanol 293b reduced both the forskolin- and carbachol-induced cell shrinkage, inhibition of Ca(2+)-sensitive K(+) channels with charybdotoxin strongly inhibited the cell volume decrease after carbachol, but not after forskolin stimulation. Carbachol 269-278 potassium voltage-gated channel subfamily KQT member 1 Oryctolagus cuniculus 63-68 17213482-6 2007 Treatment with ANG-(1-7) or AVE-0991 also prevented the diabetes-induced abnormal vascular responsiveness to norepinephrine, endothelin-1, angiotensin II, carbachol, and histamine in the perfused mesenteric bed and isolated carotid and renal arteries. Carbachol 155-164 angiopoietin 1 Homo sapiens 15-23 17310102-9 2007 PRL attenuated the increase in intracellular Ca(2+) that was caused by stimulation of isolated colonic crypts with carbachol. Carbachol 115-124 prolactin Mus musculus 0-3 16868751-3 2007 In comparison with the control animals, all Cftr (TgH(neoim)1Hgu) congenic mice had a distinctly reduced basal chloride secretion and a reduced chloride secretion after stimulation with carbachol and forskolin. Carbachol 186-195 cystic fibrosis transmembrane conductance regulator Mus musculus 44-48 17268693-8 2007 The response of the EBs to carbachol was more in the bFGF group than 7+14 days. Carbachol 27-36 fibroblast growth factor 2 Mus musculus 53-57 16899552-1 2007 EGF inhibits carbachol-induced chloride secretion by regulating a basolateral potassium channel via phosphatidylinositol 3-kinase (PI 3-kinase) and PKCepsilon activation. Carbachol 13-22 protein kinase C epsilon Homo sapiens 148-158 16899552-2 2007 Although both EGF and carbachol cause tyrosine phosphorylation of p85 of PI 3-kinase, only EGF activates the enzyme. Carbachol 22-31 phosphoinositide-3-kinase regulatory subunit 2 Homo sapiens 66-69 16899552-4 2007 Our present study examined whether the differential effects of carbachol and EGF on PI 3-kinase activity correspond to varying phosphorylation of p85, and the mechanisms and consequences. Carbachol 63-72 phosphoinositide-3-kinase regulatory subunit 2 Homo sapiens 146-149 16899552-8 2007 Both tyrosine and serine residues of p85 were phosphorylated by carbachol, whereas EGF induced only tyrosine phosphorylation. Carbachol 64-73 phosphoinositide-3-kinase regulatory subunit 2 Homo sapiens 37-40 16899552-9 2007 Moreover, EGF abolished carbachol-induced serine phosphorylation of p85 and activated PP2A without affecting PP1. Carbachol 24-33 phosphoinositide-3-kinase regulatory subunit 2 Homo sapiens 68-71 16899552-9 2007 Moreover, EGF abolished carbachol-induced serine phosphorylation of p85 and activated PP2A without affecting PP1. Carbachol 24-33 protein phosphatase 2 phosphatase activator Homo sapiens 86-90 16899552-12 2007 Although carbachol recruits p85, it phosphorylates both serine and tyrosine residues so that the lipid kinase of PI 3-kinase is inhibited. Carbachol 9-18 phosphoinositide-3-kinase regulatory subunit 2 Homo sapiens 28-31 16868751-3 2007 In comparison with the control animals, all Cftr (TgH(neoim)1Hgu) congenic mice had a distinctly reduced basal chloride secretion and a reduced chloride secretion after stimulation with carbachol and forskolin. Carbachol 186-195 carboxylesterase 1D Mus musculus 50-53 16782699-4 2006 The relaxation response to (-)-isoprenaline in carbachol-contracted small intestinal tissue segments was reduced in caveolin-1 knockout mice (cav1(-/-)) compared with their genetic controls (cav1(+/+)). Carbachol 47-56 caveolin 1, caveolae protein Mus musculus 116-126 17005918-8 2007 In separate groups of ApoE-/- mice, NCX 6550 significantly enhanced endothelium-dependent relaxation to carbachol in aortic segments precon-tracted with phenylephrine (-logEC(50), 6.37 +/- 0.37) compared with both vehicle-treated (-logEC50, 5.81 +/- 0.15; P < 0.001) and pravastatin-treated (-logEC50, 5.57 +/- 0.45; P < 0.05) mice. Carbachol 104-113 T cell leukemia, homeobox 2 Mus musculus 36-39 17026971-5 2006 Carbachol stimulation of SH-SY5Y cells dose-dependently stimulated the activation of the transcription factors NFkappaB and AP-1 as revealed by electrophoretic mobility shift assay (EMSA), while pre-exposure of SH-SY5Y cells for 24 h with 1 ng/ml IL-1beta completely suppressed the carbachol response. Carbachol 0-9 JunB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 124-128 17026971-5 2006 Carbachol stimulation of SH-SY5Y cells dose-dependently stimulated the activation of the transcription factors NFkappaB and AP-1 as revealed by electrophoretic mobility shift assay (EMSA), while pre-exposure of SH-SY5Y cells for 24 h with 1 ng/ml IL-1beta completely suppressed the carbachol response. Carbachol 0-9 interleukin 1 alpha Homo sapiens 247-255 17026971-5 2006 Carbachol stimulation of SH-SY5Y cells dose-dependently stimulated the activation of the transcription factors NFkappaB and AP-1 as revealed by electrophoretic mobility shift assay (EMSA), while pre-exposure of SH-SY5Y cells for 24 h with 1 ng/ml IL-1beta completely suppressed the carbachol response. Carbachol 282-291 JunB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 124-128 17026971-6 2006 mAChR-mediated enhancements of AChE activity by carbachol were impaired following pre-exposure of SH-SY5Y cells with IL-1beta, already detectable at a concentration of 1 ng/ml and 1 h of exposure time. Carbachol 48-57 acetylcholinesterase (Cartwright blood group) Homo sapiens 31-35 17026971-6 2006 mAChR-mediated enhancements of AChE activity by carbachol were impaired following pre-exposure of SH-SY5Y cells with IL-1beta, already detectable at a concentration of 1 ng/ml and 1 h of exposure time. Carbachol 48-57 interleukin 1 alpha Homo sapiens 117-125 16838106-4 2007 TRPC7-transfected HEK293 cells showed an augmentation of carbachol-induced intracellular Ca2+ transient, which was attenuated under a Ca2+-free condition or in the presence of SK&F96365 (a Ca2+-permeable channel blocker). Carbachol 57-66 transient receptor potential cation channel subfamily C member 7 Homo sapiens 0-5 16838106-4 2007 TRPC7-transfected HEK293 cells showed an augmentation of carbachol-induced intracellular Ca2+ transient, which was attenuated under a Ca2+-free condition or in the presence of SK&F96365 (a Ca2+-permeable channel blocker). Carbachol 57-66 carbonic anhydrase 2 Homo sapiens 89-92 16838106-4 2007 TRPC7-transfected HEK293 cells showed an augmentation of carbachol-induced intracellular Ca2+ transient, which was attenuated under a Ca2+-free condition or in the presence of SK&F96365 (a Ca2+-permeable channel blocker). Carbachol 57-66 carbonic anhydrase 2 Homo sapiens 134-137 16838106-4 2007 TRPC7-transfected HEK293 cells showed an augmentation of carbachol-induced intracellular Ca2+ transient, which was attenuated under a Ca2+-free condition or in the presence of SK&F96365 (a Ca2+-permeable channel blocker). Carbachol 57-66 carbonic anhydrase 2 Homo sapiens 134-137 16925472-5 2007 Immunohistochemistry showed that carbachol induced an increase of neuronal nitric oxide synthase immunoreactivity (nNOS-IR) in the LC and renal proximal tubular cells. Carbachol 33-42 nitric oxide synthase 1 Homo sapiens 66-96 16925472-5 2007 Immunohistochemistry showed that carbachol induced an increase of neuronal nitric oxide synthase immunoreactivity (nNOS-IR) in the LC and renal proximal tubular cells. Carbachol 33-42 nitric oxide synthase 1 Homo sapiens 115-119 16925472-7 2007 The same was true for carbachol-induced increase of nNOS-IR in the LC and PCT. Carbachol 22-31 nitric oxide synthase 1 Homo sapiens 52-56 17035232-5 2006 CCK-8, carbachol, and bombesin, but not VIP/secretin, decreased c-Met. Carbachol 7-16 MET proto-oncogene, receptor tyrosine kinase Rattus norvegicus 64-69 17065500-3 2006 The effects of PGF(2)alpha and CCh on the phosphorylation of myosin light chain (MLC) were also analyzed. Carbachol 31-34 myosin light chain 1 Sus scrofa 81-84 16782699-4 2006 The relaxation response to (-)-isoprenaline in carbachol-contracted small intestinal tissue segments was reduced in caveolin-1 knockout mice (cav1(-/-)) compared with their genetic controls (cav1(+/+)). Carbachol 47-56 caveolin 1, caveolae protein Mus musculus 142-146 17092423-8 2006 While in carbachol treatment group, the number of splenic DC had no significant change, the expression of IL-1 beta was down-regulated, dramatically. Carbachol 9-18 interleukin 1 beta Mus musculus 106-115 16675744-9 2006 We conclude that NBC in the murine colon is thus activated by carbachol, consistent with its presumed function as an anion uptake pathway during intestinal anion secretion, but that the signal transductions pathways are distinct from those involved in the cholinergic activation of renal NBC1. Carbachol 62-71 solute carrier family 4 (anion exchanger), member 4 Mus musculus 17-20 16675744-9 2006 We conclude that NBC in the murine colon is thus activated by carbachol, consistent with its presumed function as an anion uptake pathway during intestinal anion secretion, but that the signal transductions pathways are distinct from those involved in the cholinergic activation of renal NBC1. Carbachol 62-71 solute carrier family 4 (anion exchanger), member 4 Mus musculus 288-292 17005856-3 2006 To better understand the synaptic mechanisms involved in gamma oscillogenesis, we recorded action potentials and synaptic currents in distinct types of anatomically identified CA3 neurons during carbachol-induced (20-25 microM) gamma oscillations in rat hippocampal slices. Carbachol 195-204 carbonic anhydrase 3 Rattus norvegicus 176-179 16741513-9 2006 Finally, transient receptor potential canonical 7 (TRPC7) specific knockdown by antisense oligonucleotides led to a decrease in ATP- and CCh-induced calcium entry, as well as OAG-evoked current. Carbachol 137-140 transient receptor potential cation channel subfamily C member 7 Homo sapiens 9-49 16809362-10 2006 Synaptically activating waves in the presence of low concentrations of carbachol, which probably increased the tonic level of IP3 throughout the cell, enhanced the extent of propagation and generated waves that invaded the soma, as long as low-affinity indicators were used to detect the [Ca2+]i changes. Carbachol 71-80 carbonic anhydrase 2 Rattus norvegicus 289-292 17033098-12 2006 The carbachol-induced corticosterone response was significantly increased by pretreatment with nNOS inhibitor L-NNA and was considerably reduced by indomethacin, a general COX inhibitor. Carbachol 4-13 nitric oxide synthase 1 Rattus norvegicus 95-99 16806302-5 2006 Extracellular recordings from CA1 and CA3 regions suggest that carbachol-mediated population activity was regionalized in our preparations. Carbachol 63-72 carbonic anhydrase 1 Mus musculus 30-33 16806302-5 2006 Extracellular recordings from CA1 and CA3 regions suggest that carbachol-mediated population activity was regionalized in our preparations. Carbachol 63-72 carbonic anhydrase 3 Mus musculus 38-41 16951552-0 2006 Involvement of the phospholipase C beta1 pathway in desensitization of the carbachol-activated nonselective cationic current in murine gastric myocytes. Carbachol 75-84 phospholipase C, beta 1 Mus musculus 19-40 16938132-7 2006 Using rat tracheal rings, we observed that the activation of CFTR by benzoquinolizinium derivatives in TSMC leads to CFTRinh-172-sensitive bronchodilation after constriction with carbachol. Carbachol 179-188 CF transmembrane conductance regulator Rattus norvegicus 61-65 16741513-9 2006 Finally, transient receptor potential canonical 7 (TRPC7) specific knockdown by antisense oligonucleotides led to a decrease in ATP- and CCh-induced calcium entry, as well as OAG-evoked current. Carbachol 137-140 transient receptor potential cation channel subfamily C member 7 Homo sapiens 51-56 16865060-9 2006 RESULTS: The results of this study suggest that blockade of chemokine receptors significantly potentiated erythropoietin- and quercetin-induced inhibition of carbachol-induced bronchoconstriction (P<0.05) compared with the control group. Carbachol 158-167 erythropoietin Mus musculus 106-120 16765919-8 2006 Activation of TRPC1 protein by either thapsigargin or carbachol further protected SH-SY5Y cells from salsolinol treatments. Carbachol 54-63 transient receptor potential cation channel subfamily C member 1 Homo sapiens 14-19 16185843-4 2006 HGF and EGF stimulated total Gab1 tyrosine phosphorylation (TyrP) and TyrP of Gab1 phospho-specific sites (Y307, Y627), but not other pancreatic growth factors, GI GPCRs (CCK, bombesin, carbachol, VIP, secretin), or agents directly activating PKC or increasing Ca2+. Carbachol 186-195 hepatocyte growth factor Rattus norvegicus 0-3 16877402-7 2006 Inhibition of PKC partially inhibited carbachol-stimulated MAPK activation while completely inhibiting phenylephrine- and EGF-stimulated MAPK activation. Carbachol 38-47 mitogen activated protein kinase 3 Rattus norvegicus 59-63 16877402-8 2006 Chelation of Ca(2+) also partially inhibited carbachol-stimulated MAPK with no effect on phenylephrine- and EGF-stimulated MAPK activation. Carbachol 45-54 mitogen activated protein kinase 3 Rattus norvegicus 66-70 16877402-9 2006 Carbachol increased the phosphorylation of Pyk2 on tyrosine 402 and c-src on tyrosine 416 in a time-dependent manner. Carbachol 0-9 protein tyrosine kinase 2 beta Rattus norvegicus 43-47 16877402-9 2006 Carbachol increased the phosphorylation of Pyk2 on tyrosine 402 and c-src on tyrosine 416 in a time-dependent manner. Carbachol 0-9 SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus 68-73 16877402-10 2006 The c-src inhibitor PP1 inhibited carbachol-stimulated phosphorylation of Pyk2. Carbachol 34-43 SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus 4-9 16877402-10 2006 The c-src inhibitor PP1 inhibited carbachol-stimulated phosphorylation of Pyk2. Carbachol 34-43 neuropeptide Y receptor Y4 Rattus norvegicus 20-23 16877402-10 2006 The c-src inhibitor PP1 inhibited carbachol-stimulated phosphorylation of Pyk2. Carbachol 34-43 protein tyrosine kinase 2 beta Rattus norvegicus 74-78 16467403-5 2006 In a test of the specific hypothesis that the level of APP intracellular domain (AICD), the intracellular fragment of the beta-amyloid precursor protein (APP) resulting from gamma-secretase cleavage, can modulate the Ca(2+) content of the endoplasmic reticulum, we were unable to demonstrate any effect of APP small interfering RNA on the magnitude of carbachol-induced intracellular calcium release in HSG cells. Carbachol 352-361 amyloid beta precursor protein Homo sapiens 122-152 16879495-4 2006 Carbachol stimulates DNA synthesis, InsP and the expression of CD40. Carbachol 0-9 CD40 molecule Homo sapiens 63-67 16879498-10 2006 Tissue incubation with TNF-alpha (100 ng ml(-1))/IL-1beta (10 ng ml(-1)) increased in vitro tracheal responsiveness to carbachol. Carbachol 119-128 tumor necrosis factor Rattus norvegicus 23-32 16879498-10 2006 Tissue incubation with TNF-alpha (100 ng ml(-1))/IL-1beta (10 ng ml(-1)) increased in vitro tracheal responsiveness to carbachol. Carbachol 119-128 interleukin 1 beta Rattus norvegicus 49-57 16185843-4 2006 HGF and EGF stimulated total Gab1 tyrosine phosphorylation (TyrP) and TyrP of Gab1 phospho-specific sites (Y307, Y627), but not other pancreatic growth factors, GI GPCRs (CCK, bombesin, carbachol, VIP, secretin), or agents directly activating PKC or increasing Ca2+. Carbachol 186-195 GRB2-associated binding protein 1 Rattus norvegicus 29-33 16756988-5 2006 Importantly, RGS2 selectively inhibited Gq/11 signaling, whereas RGS3, RGS4 and RGS5 had the capacity to regulate both Gq/11 and Gi/o signaling (carbachol-induced cAMP inhibition). Carbachol 145-154 regulator of G protein signaling 2 Homo sapiens 13-17 16756988-5 2006 Importantly, RGS2 selectively inhibited Gq/11 signaling, whereas RGS3, RGS4 and RGS5 had the capacity to regulate both Gq/11 and Gi/o signaling (carbachol-induced cAMP inhibition). Carbachol 145-154 regulator of G protein signaling 3 Homo sapiens 65-69 16756988-5 2006 Importantly, RGS2 selectively inhibited Gq/11 signaling, whereas RGS3, RGS4 and RGS5 had the capacity to regulate both Gq/11 and Gi/o signaling (carbachol-induced cAMP inhibition). Carbachol 145-154 regulator of G protein signaling 4 Homo sapiens 71-75 16756988-5 2006 Importantly, RGS2 selectively inhibited Gq/11 signaling, whereas RGS3, RGS4 and RGS5 had the capacity to regulate both Gq/11 and Gi/o signaling (carbachol-induced cAMP inhibition). Carbachol 145-154 regulator of G protein signaling 5 Homo sapiens 80-84 16631594-2 2006 Using Chinese hamster ovary (CHO) cells stably expressing GAR-3b, the major alternatively spliced isoform of GAR-3, we observed that carbachol stimulated cyclic AMP (cAMP) production in a dose- and time-dependent manner. Carbachol 133-142 Muscarinic acetylcholine receptor gar-3 Caenorhabditis elegans 58-63 16773404-10 2006 Carbachol and ATP, which both induce a Ca(2+) release from internal Ca(2+) stores, also increased the NHE3 activity. Carbachol 0-9 solute carrier family 9 member A3 Sus scrofa 102-106 16741047-7 2006 Serum withdrawal from the incubation medium as well as addition of carbachol or PMA for 24 hrs also led to a significant reduction of the levels of ECE-1 protein in NB7 cells. Carbachol 67-76 endothelin converting enzyme 1 Homo sapiens 148-153 16620785-8 2006 Stimulation of muscarinic receptors with carbachol in SH-SY5Y cells also activated AMPK and transiently caused dephosphorylation of Akt. Carbachol 41-50 AKT serine/threonine kinase 1 Homo sapiens 132-135 16794864-4 2006 When Chinese hamster ovary cells stably expressing GAR-3 were treated with carbachol, GAR-3 was internalized in a dose- and time-dependent manner. Carbachol 75-84 Muscarinic acetylcholine receptor gar-3 Caenorhabditis elegans 51-56 16794864-4 2006 When Chinese hamster ovary cells stably expressing GAR-3 were treated with carbachol, GAR-3 was internalized in a dose- and time-dependent manner. Carbachol 75-84 Muscarinic acetylcholine receptor gar-3 Caenorhabditis elegans 86-91 16794864-5 2006 Approximately 60% of the cell surface receptor was internalized by exposure to 1 mM carbachol for 1 h. Carbachol-induced GAR-3 internalization was suppressed by treatment with hypertonic sucrose, which blocks the formation of clathrin-coated pits. Carbachol 84-93 Muscarinic acetylcholine receptor gar-3 Caenorhabditis elegans 121-126 16794864-5 2006 Approximately 60% of the cell surface receptor was internalized by exposure to 1 mM carbachol for 1 h. Carbachol-induced GAR-3 internalization was suppressed by treatment with hypertonic sucrose, which blocks the formation of clathrin-coated pits. Carbachol 103-112 Muscarinic acetylcholine receptor gar-3 Caenorhabditis elegans 121-126 16794864-6 2006 Overexpression of a dominant-negative dynamin mutant (DynK44A), but not of a dominant-negative beta-arrestin mutant (Arr319-418), substantially inhibited carbachol-induced internalization of GAR-3. Carbachol 154-163 Muscarinic acetylcholine receptor gar-3 Caenorhabditis elegans 191-196 16520739-3 2006 Stimulation of PAR-2 by trypsin-induced relaxation of carbachol- and KCl-induced contractions in normal rat colonic smooth muscle was completely resolved by tissue pretreatment with apamin, but not by pretreatment with l-NMMA or a cocktail of neuronal blockers (tetrodotoxin, hexamethonium and propranolol). Carbachol 54-63 F2R like trypsin receptor 1 Rattus norvegicus 15-20 16393138-3 2006 In the present study, we used subsarcolemmal- and mitochondrial-targeted aequorin to study the effect of the antiapoptotic Bcl-2 protein overexpression on carbachol-induced near-plasma membrane and mitochondrial calcium responses in myotubes derived from control C57 and dystrophic (mdx) mice. Carbachol 155-164 B cell leukemia/lymphoma 2 Mus musculus 123-128 16510245-6 2006 Microinjections of carbachol into the LSV and corticotropin-releasing factor into the MeA, and intracerebroventricular injection of hypertonic saline, activated AHA angiotensin II-sensitive neurons. Carbachol 19-28 angiotensinogen Rattus norvegicus 165-179 16213169-8 2006 Also, hM1 receptors elicited phosphoinositide hydrolysis to carbachol once expressed at the plasma membrane and the pharmacology of the response varied depending upon the concentration of AP21998 used to cause plasma membrane expression. Carbachol 60-69 cholinergic receptor muscarinic 1 Homo sapiens 6-9 16306123-3 2006 TRPC5 was initially activated by muscarinic stimulation with 50 microM carbachol and then decayed rapidly even in the presence of carbachol. Carbachol 71-80 transient receptor potential cation channel subfamily C member 5 Homo sapiens 0-5 16306123-3 2006 TRPC5 was initially activated by muscarinic stimulation with 50 microM carbachol and then decayed rapidly even in the presence of carbachol. Carbachol 130-139 transient receptor potential cation channel subfamily C member 5 Homo sapiens 0-5 16339998-2 2006 Carbachol (1 microM) induced significant increases in the expression of cyclooxygenase (COX)-1, COX-2, IL-8, and plasminogen activator, urokinase type (PLAU) to levels ranging from 1.3- to 3.1-fold of control. Carbachol 0-9 cytochrome c oxidase subunit I Bos taurus 72-94 16339998-2 2006 Carbachol (1 microM) induced significant increases in the expression of cyclooxygenase (COX)-1, COX-2, IL-8, and plasminogen activator, urokinase type (PLAU) to levels ranging from 1.3- to 3.1-fold of control. Carbachol 0-9 cytochrome c oxidase subunit II Bos taurus 96-101 16339998-2 2006 Carbachol (1 microM) induced significant increases in the expression of cyclooxygenase (COX)-1, COX-2, IL-8, and plasminogen activator, urokinase type (PLAU) to levels ranging from 1.3- to 3.1-fold of control. Carbachol 0-9 C-X-C motif chemokine ligand 8 Bos taurus 103-107 16339998-2 2006 Carbachol (1 microM) induced significant increases in the expression of cyclooxygenase (COX)-1, COX-2, IL-8, and plasminogen activator, urokinase type (PLAU) to levels ranging from 1.3- to 3.1-fold of control. Carbachol 0-9 plasminogen activator, urokinase Bos taurus 152-156 16469785-4 2006 Mutant NCS-1 does not inhibit surface-expression of TRPC5 but generally suppresses channel activity, irrespective of whether it is evoked by carbachol, store depletion, lanthanides or elevated intracellular calcium. Carbachol 141-150 neuronal calcium sensor 1 Rattus norvegicus 7-12 16227319-6 2006 In both nontransformed human cultured airway smooth muscle cells and cells transduced with wild-type human Lyn kinase, carbachol increased Lyn kinase activity. Carbachol 119-128 LYN proto-oncogene, Src family tyrosine kinase Homo sapiens 107-110 16227319-6 2006 In both nontransformed human cultured airway smooth muscle cells and cells transduced with wild-type human Lyn kinase, carbachol increased Lyn kinase activity. Carbachol 119-128 LYN proto-oncogene, Src family tyrosine kinase Homo sapiens 139-142 16227319-7 2006 Pertussis toxin pretreatment failed to block carbachol activation of Lyn kinase but did attenuate the carbachol-induced increase in ERK/MAPK phosphorylation. Carbachol 102-111 EPH receptor B2 Homo sapiens 132-135 16565731-0 2006 Involvement of Rho kinase and protein kinase C in carbachol-induced calcium sensitization in beta-escin skinned rat and guinea-pig bladders. Carbachol 50-59 Rho-associated coiled-coil containing protein kinase 2 Rattus norvegicus 15-25 16565731-12 2006 The Rho kinase (ROK) inhibitor Y-27632 (5 microM) added in the presence of CCh reversed the calcium sensitization in rat bladder, whereas a transient contraction followed by a relaxation to a level not significantly different from the CCh contraction was seen in both guinea-pig bladder and taenia caecum. Carbachol 75-78 Rho-associated coiled-coil containing protein kinase 2 Rattus norvegicus 4-14 16565731-12 2006 The Rho kinase (ROK) inhibitor Y-27632 (5 microM) added in the presence of CCh reversed the calcium sensitization in rat bladder, whereas a transient contraction followed by a relaxation to a level not significantly different from the CCh contraction was seen in both guinea-pig bladder and taenia caecum. Carbachol 75-78 Rho-associated coiled-coil containing protein kinase 2 Rattus norvegicus 16-19 16439036-2 2006 Here we present evidence that incubation of oligodendrocyte progenitors, deprived of growth factor, with the acetylcholine analog carbachol significantly reduced cell death by apoptosis and blocked caspase-3 cleavage. Carbachol 130-139 caspase 3 Homo sapiens 198-207 16439036-4 2006 Activation of Akt by carbachol was antagonized by atropine and inhibited by LY294002 and PP2. Carbachol 21-30 AKT serine/threonine kinase 1 Homo sapiens 14-17 16439036-4 2006 Activation of Akt by carbachol was antagonized by atropine and inhibited by LY294002 and PP2. Carbachol 21-30 neuropeptide Y receptor Y6 (pseudogene) Homo sapiens 89-92 16439036-5 2006 The Src-like tyrosine kinase inhibitor, PP2, also reduced carbachol stimulation of extracellular signal-regulated kinases 1/2 and cAMP-response element binding protein in a dose-dependent manner. Carbachol 58-67 neuropeptide Y receptor Y6 (pseudogene) Homo sapiens 40-43 16439036-5 2006 The Src-like tyrosine kinase inhibitor, PP2, also reduced carbachol stimulation of extracellular signal-regulated kinases 1/2 and cAMP-response element binding protein in a dose-dependent manner. Carbachol 58-67 mitogen-activated protein kinase 3 Homo sapiens 83-125 16439036-6 2006 Furthermore, carbachol increased tyrosine-phosphorylation of Fyn, a member of the Src-like tyrosine kinases. Carbachol 13-22 FYN proto-oncogene, Src family tyrosine kinase Homo sapiens 61-64 16546360-2 2006 Carbachol and PGE(2) increase endogenous PGE(2) production and the COX-1 mRNA levels by activation of PLA(2)s. The COX-1 and COX-2 activity participated in the production of PGE(2) triggered by exogenous PGE(2). Carbachol 0-9 cytochrome c oxidase I, mitochondrial Rattus norvegicus 67-72 16546360-2 2006 Carbachol and PGE(2) increase endogenous PGE(2) production and the COX-1 mRNA levels by activation of PLA(2)s. The COX-1 and COX-2 activity participated in the production of PGE(2) triggered by exogenous PGE(2). Carbachol 0-9 cytochrome c oxidase I, mitochondrial Rattus norvegicus 115-120 16546360-2 2006 Carbachol and PGE(2) increase endogenous PGE(2) production and the COX-1 mRNA levels by activation of PLA(2)s. The COX-1 and COX-2 activity participated in the production of PGE(2) triggered by exogenous PGE(2). Carbachol 0-9 cytochrome c oxidase II, mitochondrial Rattus norvegicus 125-130 16680828-4 2006 Carbachol-stimulated glutamate release was prolonged by perfusion of the selective D2 dopamine receptor antagonist raclopride (100 microM) into the SN and was attenuated by the perfusion of the selective D2-like receptor agonist quinpirole (10 microM). Carbachol 0-9 dopamine receptor D2 Rattus norvegicus 83-103 16236826-0 2006 Ouabain potentiates the activation of ERK1/2 by carbachol in parotid gland epithelial cells; inhibition of ERK1/2 reduces Na(+)-K(+)-ATPase activity. Carbachol 48-57 mitogen activated protein kinase 3 Rattus norvegicus 38-44 16236826-8 2006 Although ERK1/2 is only modestly phosphorylated when cells are exposed to 1 mM ouabain or 10(-6) M carbachol, the combination of these agents promotes ERK1/2 phosphorylation to near-maximal levels achieved by a log order carbachol concentration. Carbachol 99-108 mitogen activated protein kinase 3 Rattus norvegicus 9-15 16236826-8 2006 Although ERK1/2 is only modestly phosphorylated when cells are exposed to 1 mM ouabain or 10(-6) M carbachol, the combination of these agents promotes ERK1/2 phosphorylation to near-maximal levels achieved by a log order carbachol concentration. Carbachol 99-108 mitogen activated protein kinase 3 Rattus norvegicus 151-157 16236826-8 2006 Although ERK1/2 is only modestly phosphorylated when cells are exposed to 1 mM ouabain or 10(-6) M carbachol, the combination of these agents promotes ERK1/2 phosphorylation to near-maximal levels achieved by a log order carbachol concentration. Carbachol 221-230 mitogen activated protein kinase 3 Rattus norvegicus 9-15 16236826-8 2006 Although ERK1/2 is only modestly phosphorylated when cells are exposed to 1 mM ouabain or 10(-6) M carbachol, the combination of these agents promotes ERK1/2 phosphorylation to near-maximal levels achieved by a log order carbachol concentration. Carbachol 221-230 mitogen activated protein kinase 3 Rattus norvegicus 151-157 16236826-11 2006 In addition, inhibition of ERK1/2 reduces Na(+)-K(+)-ATPase activity (measured as stimulation of Qo(2) by carbachol and the cationophore nystatin). Carbachol 106-115 mitogen activated protein kinase 3 Rattus norvegicus 27-33 15979279-4 2006 Western blotting analysis using a phosphospecific anti-Akt antibody revealed a dose- and time-dependent increase in Akt phosphorylation in cells stimulated with mAChR specific agonist carbachol (CCh). Carbachol 184-193 AKT serine/threonine kinase 1 Rattus norvegicus 55-58 15979279-4 2006 Western blotting analysis using a phosphospecific anti-Akt antibody revealed a dose- and time-dependent increase in Akt phosphorylation in cells stimulated with mAChR specific agonist carbachol (CCh). Carbachol 184-193 AKT serine/threonine kinase 1 Rattus norvegicus 116-119 15979279-4 2006 Western blotting analysis using a phosphospecific anti-Akt antibody revealed a dose- and time-dependent increase in Akt phosphorylation in cells stimulated with mAChR specific agonist carbachol (CCh). Carbachol 195-198 AKT serine/threonine kinase 1 Rattus norvegicus 55-58 15979279-4 2006 Western blotting analysis using a phosphospecific anti-Akt antibody revealed a dose- and time-dependent increase in Akt phosphorylation in cells stimulated with mAChR specific agonist carbachol (CCh). Carbachol 195-198 AKT serine/threonine kinase 1 Rattus norvegicus 116-119 15979279-5 2006 Co-stimulation with CCh and NGF resulted in augmentation of Akt activity in a pertussis toxin (PTX)-sensitive manner, suggesting that M4 mAChR, but not M1 and M5 mAChRs, was associated with this synergistic Akt activation. Carbachol 20-23 AKT serine/threonine kinase 1 Rattus norvegicus 60-63 15979279-5 2006 Co-stimulation with CCh and NGF resulted in augmentation of Akt activity in a pertussis toxin (PTX)-sensitive manner, suggesting that M4 mAChR, but not M1 and M5 mAChRs, was associated with this synergistic Akt activation. Carbachol 20-23 AKT serine/threonine kinase 1 Rattus norvegicus 207-210 15979279-7 2006 Additional experiments showed that CCh treatment augmented NGF-induced phosphorylation and degradation of the Akt-regulated translation regulator tuberin. Carbachol 35-38 AKT serine/threonine kinase 1 Rattus norvegicus 110-113 15979279-7 2006 Additional experiments showed that CCh treatment augmented NGF-induced phosphorylation and degradation of the Akt-regulated translation regulator tuberin. Carbachol 35-38 TSC complex subunit 2 Rattus norvegicus 146-153 16553779-6 2006 In contrast, SNX-482 (100 nm), a selective antagonist of R-type calcium channels containing the alpha1E (Cav2.3) subunit, attenuated carbachol inhibition of the somatic spike-evoked calcium transient. Carbachol 133-142 calcium channel, voltage-dependent, R type, alpha 1E subunit Mus musculus 96-103 16553779-6 2006 In contrast, SNX-482 (100 nm), a selective antagonist of R-type calcium channels containing the alpha1E (Cav2.3) subunit, attenuated carbachol inhibition of the somatic spike-evoked calcium transient. Carbachol 133-142 calcium channel, voltage-dependent, R type, alpha 1E subunit Mus musculus 105-111 16567411-7 2006 Exogenous expression of antisense RNA encoding the rat canonical-transient receptor potential Ca2+ channel subtype 6 (TRPC6) or the N-terminal domain of TRPC6 blocks carbachol-stimulated Ba2+ influx in PC12D cells. Carbachol 166-175 transient receptor potential cation channel, subfamily C, member 6 Rattus norvegicus 55-116 16567411-7 2006 Exogenous expression of antisense RNA encoding the rat canonical-transient receptor potential Ca2+ channel subtype 6 (TRPC6) or the N-terminal domain of TRPC6 blocks carbachol-stimulated Ba2+ influx in PC12D cells. Carbachol 166-175 transient receptor potential cation channel, subfamily C, member 6 Rattus norvegicus 118-123 16567411-7 2006 Exogenous expression of antisense RNA encoding the rat canonical-transient receptor potential Ca2+ channel subtype 6 (TRPC6) or the N-terminal domain of TRPC6 blocks carbachol-stimulated Ba2+ influx in PC12D cells. Carbachol 166-175 transient receptor potential cation channel, subfamily C, member 6 Rattus norvegicus 153-158 16316991-4 2006 Employing caspase-3 for IP3R cleavage, we show that Cch promotes polyubiquitination in the N-terminal domain and monoubiquitination in the C-terminal domain. Carbachol 52-55 caspase-3 Cricetulus griseus 10-19 16227319-9 2006 The present study shows that Lyn kinase is expressed in human cultured airway smooth muscle cells at both the mRNA and protein levels and that carbachol, an M2 muscarinic receptor agonist in these cells, activates Lyn kinase by a pertussis toxin-insensitive signaling pathway. Carbachol 143-152 LYN proto-oncogene, Src family tyrosine kinase Homo sapiens 214-217 16243961-11 2006 Inhibition of rho kinase, PKA, and PKG with N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide.2HCl (H89) reduces carbachol maximum and carbachol potency. Carbachol 123-132 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 26-29 16403946-6 2006 The abnormal vascular responsiveness to endothelin-1, carbachol, and sodium nitroprusside in perfused mesenteric vascular bed of SHR-L-NAME was improved by ANG-(1-7) or captopril, with no additive effect of ANG-(1-7) + captopril. Carbachol 54-63 angiogenin Rattus norvegicus 156-164 16472591-2 2006 The present study was designed to examine the role of SLC26A6 in prostaglandin E(2) (PGE(2))-, forskolin-, and carbachol-induced duodenal HCO(3)(-) secretion. Carbachol 111-120 solute carrier family 26, member 6 Mus musculus 54-61 16472591-4 2006 RESULTS: Basal HCO(3)(-) secretion was diminished by 20%, PGE(2)-stimulated HCO(3)(-) secretory response by 59%, and carbachol-stimulated response was reduced by 35% in SLC26A6-/- compared with +/+ duodenal mucosa, whereas the forskolin-stimulated HCO(3)(-) secretory response was not different. Carbachol 117-126 solute carrier family 26, member 6 Mus musculus 169-176 16243961-9 2006 Inhibition of rho kinase, protein kinase A (PKA), and protein kinase G (PKG) with 1-(5-isoquinolinesulfonyl)-homopiperazine.HCl (HA-1077) reduces the carbachol maximal contraction, carbachol potency, and darifenacin affinity. Carbachol 150-159 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 26-42 16243961-9 2006 Inhibition of rho kinase, protein kinase A (PKA), and protein kinase G (PKG) with 1-(5-isoquinolinesulfonyl)-homopiperazine.HCl (HA-1077) reduces the carbachol maximal contraction, carbachol potency, and darifenacin affinity. Carbachol 150-159 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 44-47 16243962-10 2006 Inhibition of rho kinase, protein kinase A (PKA), and protein kinase G (PKG) with 1-(5-isoquinolinesulfonyl)-homopiperazine.HCl (HA-1077) reduces the carbachol maximum and potency. Carbachol 150-159 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 26-42 16243962-10 2006 Inhibition of rho kinase, protein kinase A (PKA), and protein kinase G (PKG) with 1-(5-isoquinolinesulfonyl)-homopiperazine.HCl (HA-1077) reduces the carbachol maximum and potency. Carbachol 150-159 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 44-47 16456221-5 2006 Both the muscarinic GPCR agonist carbachol and the ER Ca2+-adenosine triphosphate inhibitor thapsigargin (Tg) induced [Ca2+]i oscillations in NT2 cells. Carbachol 33-42 C-X-C motif chemokine receptor 6 Homo sapiens 20-24 21186577-6 2006 CONCLUSION: The results above suggest that i. c. v. injection of cholinergic agonist carbachol can enhance the activity of adrenergic neurons and the expression of AT1 receptor in locus coeruleus. Carbachol 85-94 angiotensin II receptor, type 1a Rattus norvegicus 164-167 21186577-7 2006 The blockade of AT1 receptor may down regulate the above action induced by carbachol in locus coeruleus. Carbachol 75-84 angiotensin II receptor, type 1a Rattus norvegicus 16-19 16354194-7 2005 In the presence of the agonist carbachol, the effects of SLURP-1 on gene expression were augmented, which is in keeping with the notion that SLURP-1 acts as an allosteric agonist at the KC nAChR. Carbachol 31-40 secreted LY6/PLAUR domain containing 1 Homo sapiens 57-64 16354194-7 2005 In the presence of the agonist carbachol, the effects of SLURP-1 on gene expression were augmented, which is in keeping with the notion that SLURP-1 acts as an allosteric agonist at the KC nAChR. Carbachol 31-40 secreted LY6/PLAUR domain containing 1 Homo sapiens 141-148 16354194-7 2005 In the presence of the agonist carbachol, the effects of SLURP-1 on gene expression were augmented, which is in keeping with the notion that SLURP-1 acts as an allosteric agonist at the KC nAChR. Carbachol 31-40 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 189-194 16324225-0 2005 Restoration by VIP of the carbachol-stimulated Cl- secretion in TTX-treated guinea pig distal colon. Carbachol 26-35 VIP peptides Cavia porcellus 15-18 16324225-5 2005 However, a serosal application, not mucosal, of VIP (10(-7) M) and 8-bromo-cAMP (10(-3) M) restored the Cch-stimulated, TTX-inhibited I(sc) by 113% and 75.8%, respectively. Carbachol 104-107 VIP peptides Cavia porcellus 48-51 16183168-2 2005 In the present study, we reported that carbachol, a muscarinic cholinergic receptor agonist, regulated Bim in human SH-SY5Y neuroblastoma cells. Carbachol 39-48 BCL2 like 11 Homo sapiens 103-106 16183168-3 2005 Carbachol rapidly induced an upward gel mobility shift of Bim, which was abolished by protein phosphatase treatment, indicating an increased Bim phosphorylation by carbachol. Carbachol 0-9 BCL2 like 11 Homo sapiens 58-61 16183168-3 2005 Carbachol rapidly induced an upward gel mobility shift of Bim, which was abolished by protein phosphatase treatment, indicating an increased Bim phosphorylation by carbachol. Carbachol 0-9 BCL2 like 11 Homo sapiens 141-144 16183168-3 2005 Carbachol rapidly induced an upward gel mobility shift of Bim, which was abolished by protein phosphatase treatment, indicating an increased Bim phosphorylation by carbachol. Carbachol 164-173 BCL2 like 11 Homo sapiens 58-61 16183168-3 2005 Carbachol rapidly induced an upward gel mobility shift of Bim, which was abolished by protein phosphatase treatment, indicating an increased Bim phosphorylation by carbachol. Carbachol 164-173 BCL2 like 11 Homo sapiens 141-144 16183168-4 2005 The effect of carbachol was mimicked by the protein kinase C activator 12-myristate 13-acetate (PMA) and was blocked by the protein kinase C inhibitor rottlerin, suggesting that activation of protein kinase C was required for carbachol-induced phosphorylation of Bim. Carbachol 14-23 BCL2 like 11 Homo sapiens 263-266 16183168-4 2005 The effect of carbachol was mimicked by the protein kinase C activator 12-myristate 13-acetate (PMA) and was blocked by the protein kinase C inhibitor rottlerin, suggesting that activation of protein kinase C was required for carbachol-induced phosphorylation of Bim. Carbachol 226-235 BCL2 like 11 Homo sapiens 263-266 16183168-5 2005 Prolonged treatment with carbachol and PMA significantly decreased Bim protein levels in total cell lysates and mitrochondria. Carbachol 25-34 BCL2 like 11 Homo sapiens 67-70 16183168-6 2005 Carbachol and PMA had no effect in the transcriptional regulation of Bim, whereas the reduction of Bim by both carbachol and PMA was reversed by the proteosome inhibitors, suggesting that carbachol and PMA facilitated the proteosome-dependent Bim degradation. Carbachol 111-120 BCL2 like 11 Homo sapiens 99-102 16183168-6 2005 Carbachol and PMA had no effect in the transcriptional regulation of Bim, whereas the reduction of Bim by both carbachol and PMA was reversed by the proteosome inhibitors, suggesting that carbachol and PMA facilitated the proteosome-dependent Bim degradation. Carbachol 111-120 BCL2 like 11 Homo sapiens 99-102 16183168-6 2005 Carbachol and PMA had no effect in the transcriptional regulation of Bim, whereas the reduction of Bim by both carbachol and PMA was reversed by the proteosome inhibitors, suggesting that carbachol and PMA facilitated the proteosome-dependent Bim degradation. Carbachol 188-197 BCL2 like 11 Homo sapiens 99-102 16183168-6 2005 Carbachol and PMA had no effect in the transcriptional regulation of Bim, whereas the reduction of Bim by both carbachol and PMA was reversed by the proteosome inhibitors, suggesting that carbachol and PMA facilitated the proteosome-dependent Bim degradation. Carbachol 188-197 BCL2 like 11 Homo sapiens 99-102 16387931-9 2006 Adventitially, trypsin, thrombin and PAR4 AP (but not PAR2 AP) relaxed carbachol-toned airways after <3 min. Carbachol 71-80 coagulation factor II, thrombin Sus scrofa 24-32 16280365-2 2006 A novel finding was that brief exposure of airway smooth muscle cells to IL-4 inhibited carbachol-stimulated calcium transients. Carbachol 88-97 interleukin 4 Bos taurus 73-77 16967497-0 2006 Microinjection of carbachol in the supramammillary region suppresses CA1 pyramidal cell synaptic excitability. Carbachol 18-27 carbonic anhydrase 1 Rattus norvegicus 69-72 16967497-4 2006 It was observed that microinjection of the cholinergic muscarinic receptor agonist, carbachol (0.1 microl, concentration of either 0.0052, 0.156, or 0.625 microg/microl), evoked concentration-dependent suppression of CA1 pyramidal cell excitability that was dissociated from theta activation. Carbachol 84-93 carbonic anhydrase 1 Rattus norvegicus 217-220 16331108-6 2006 Apocynin abolished these effects of Ang II in a specific manner, as carbachol-induced increases in MAP were unaffected by the inhibition of NAD(P)H oxidase (MAP increased by 9 +/- 2 and 8 +/- 1 mmHg in the absence and presence of apocynin, respectively). Carbachol 68-77 angiotensinogen Rattus norvegicus 36-42 16785762-5 2006 RESULTS: Carbachol (a muscarinic acetylcholine agonist) and isoproterenol (a beta-adrenergic agonist) markedly activated CREB in parotid acinar cells. Carbachol 9-18 cAMP responsive element binding protein 1 Mus musculus 121-125 16785762-6 2006 Carbachol and isoproterenol-induced CREB phosphorylation was blocked by atropine (a muscarinic acetylcholine antagonist) and propranolol (a beta-adrenergic antagonist), respectively. Carbachol 0-9 cAMP responsive element binding protein 1 Mus musculus 36-40 16785762-8 2006 Moreover, carbachol- and isoproterenol-stimulated amylase secretion from parotid acinar cells was inhibited by H89 and adenoviral dominant-negative CREB. Carbachol 10-19 cAMP responsive element binding protein 1 Mus musculus 148-152 16216227-7 2005 Microinjection of the cholinoceptor agonist carbachol, the cholinesterase inhibitor physostigmine and the excitatory amino acid glutamate into the PHN caused increases in firing rate of AHA angiotensin-II-sensitive neurons in anesthetized WKY and SHR. Carbachol 44-53 angiotensinogen Rattus norvegicus 190-204 16330775-6 2005 In addition, carbachol-induced oscillations were obliterated in hippocampal slices of PLC-beta1(-/-) mice. Carbachol 13-22 phospholipase C, beta 1 Mus musculus 86-95 16141265-3 2005 By combining patch-clamp electrophysiology with local photolysis of caged carbachol to rapidly activate the alpha7-containing nAChRs in rat hippocampal CA1 stratum radiatum interneurones in slices, we describe a novel transient up-regulation of channel function. Carbachol 74-83 carbonic anhydrase 1 Rattus norvegicus 152-155 16000638-1 2005 In secretory epithelia, activation of PKC by phorbol ester and carbachol negatively regulates Cl(-) secretion, the transport event of secretory diarrhea. Carbachol 63-72 proline rich transmembrane protein 2 Homo sapiens 38-41 16000638-9 2005 Like PMA, carbachol reduced the amount of NKCC1 accessible to basolateral surface biotinylation in a PKC-epsilon-dependent manner. Carbachol 10-19 solute carrier family 12 member 2 Homo sapiens 42-47 16000638-9 2005 Like PMA, carbachol reduced the amount of NKCC1 accessible to basolateral surface biotinylation in a PKC-epsilon-dependent manner. Carbachol 10-19 proline rich transmembrane protein 2 Homo sapiens 101-104 16000638-10 2005 However, long-term exposure to carbachol did not result in degradation of NKCC1; rather, NKCC1 that was internalized after exposure to carbachol was recycled back to the cell membrane. Carbachol 31-40 solute carrier family 12 member 2 Homo sapiens 89-94 16000638-10 2005 However, long-term exposure to carbachol did not result in degradation of NKCC1; rather, NKCC1 that was internalized after exposure to carbachol was recycled back to the cell membrane. Carbachol 135-144 solute carrier family 12 member 2 Homo sapiens 89-94 16129413-4 2005 In SH-SY5Y cells expressing NPFF(2) receptors dNPA, in the presence of carbachol, stimulates Ca(2+) release from the intracellular stores. Carbachol 71-80 pro-FMRFamide-related neuropeptide FF Cricetulus griseus 28-32 16293767-6 2005 Interestingly, the increased responsiveness to CCh and OT was associated with an up-regulation of PLCbeta1 and PLCbeta3 enzymes. Carbachol 47-50 phospholipase C beta 1 Rattus norvegicus 98-106 16293767-6 2005 Interestingly, the increased responsiveness to CCh and OT was associated with an up-regulation of PLCbeta1 and PLCbeta3 enzymes. Carbachol 47-50 phospholipase C beta 3 Rattus norvegicus 111-119 16144656-7 2005 Microinjection of carbachol, physostigmine and glutamate into the PHN caused an increase in firing rate of AHA angiotensin II-sensitive neurons in anesthetized rats. Carbachol 18-27 angiotensinogen Rattus norvegicus 111-125 16144656-8 2005 The carbachol-induced increase of firing rate was inhibited by pressure application of the AT1 receptor antagonist losartan onto AHA angiotensin II-sensitive neurons. Carbachol 4-13 angiotensinogen Rattus norvegicus 133-147 15930141-5 2005 The chemical inhibitor GF-109203X (10 microM, 3 h), which inhibits PKC-alpha, -beta, -delta, and -epsilon, also elicited more potent inhibition of carbachol-stimulated secretion relative to Go-6976 (10 microM, 3 h), which inhibits only PKC-alpha and -beta. Carbachol 147-156 protein kinase C alpha type Oryctolagus cuniculus 236-255 16219687-2 2005 Here we use time-lapse confocal fluorescence microscopy and fluorescence recovery after photobleaching to investigate the changes in actin filaments located beneath the apical membrane during exocytosis evoked by the muscarinic agonist, carbachol (100 microM). Carbachol 237-246 actin Oryctolagus cuniculus 133-138 16219687-4 2005 Carbachol markedly increased apical actin filament turnover and also promoted transient actin assembly around apparent fusion intermediates. Carbachol 0-9 actin Oryctolagus cuniculus 36-41 16219687-4 2005 Carbachol markedly increased apical actin filament turnover and also promoted transient actin assembly around apparent fusion intermediates. Carbachol 0-9 actin Oryctolagus cuniculus 88-93 16219687-5 2005 Fluorescence recovery after photobleaching measurements revealed significant (P< or =0.05) increases and decreases, respectively, in mobile fraction (Mf) and turnover times (t1/2) for apical actin filaments in carbachol-stimulated acini relative to untreated acini. Carbachol 213-222 actin Oryctolagus cuniculus 194-199 16219687-6 2005 The myosin inhibitors, 2,3-butanedione monoxime (BDM, 10 mM, 15 minutes) and ML-7 (40 microM, 15 minutes), significantly decreased carbachol-stimulated secretion of bulk protein and the exogenous secretory vesicle marker, syncollin-GFP; these agents also promoted accumulation of actin-coated structures which were enriched, in transduced acini, in syncollin-GFP, confirming their identity as fusion intermediates. Carbachol 131-140 actin Oryctolagus cuniculus 280-285 15928025-11 2005 Overexpression of PANDER in mouse islets or addition of recombinant PANDER decreased insulin secretion induced by carbachol plus glucose or high potassium but not that by glucose alone. Carbachol 114-123 FAM3 metabolism regulating signaling molecule B Mus musculus 18-24 15928025-11 2005 Overexpression of PANDER in mouse islets or addition of recombinant PANDER decreased insulin secretion induced by carbachol plus glucose or high potassium but not that by glucose alone. Carbachol 114-123 FAM3 metabolism regulating signaling molecule B Mus musculus 68-74 16107881-3 2005 Here, we show that intracellular Ca2+ mobilization via the purinergic receptor agonist ATP, the muscarinic receptor agonist carbachol or the Ca(2+)-ATPase inhibitor thapsigargin leads to formation of anandamide, and subsequent TRPV1-dependent Ca2+ influx in transfected cells and sensory neurons of rat dorsal root ganglia (DRG). Carbachol 124-133 transient receptor potential cation channel, subfamily V, member 1 Rattus norvegicus 227-232 16093422-4 2005 Pretreatment with TNF-alpha (100 ng/ml) hyperpolarized the membrane (from -31.0 +/- 2.7 mV under control conditions to -61.2 +/- 3.2 mV in the presence of the cytokine) and potentiated the depolarization induced by carbachol (from 5.2 +/- 0.7 mV under control conditions to 27.5 +/- 2.0 mV in the presence of the cytokine). Carbachol 215-224 tumor necrosis factor Rattus norvegicus 18-27 16045692-11 2005 After addition of carbachol to the upper compartment, an increase of fibronectin induced chemotaxis of approximately 30% was observed, an effect abrogated by atropine. Carbachol 18-27 fibronectin 1 Homo sapiens 69-80 15937149-5 2005 Calcium entry through SOCs induced by the calcium-ATPase inhibitor thapsigargin or the receptor agonists UTP or carbachol inhibited guanylyl cyclase C-dependent cGMP accumulation. Carbachol 112-121 guanylate cyclase 2C Homo sapiens 132-150 15843439-3 2005 TRPC5 was initially activated by muscarinic stimulation using 100 microM carbachol (CCh) and then decayed rapidly even in the presence of CCh (desensitization). Carbachol 73-82 transient receptor potential cation channel subfamily C member 5 Homo sapiens 0-5 15843439-3 2005 TRPC5 was initially activated by muscarinic stimulation using 100 microM carbachol (CCh) and then decayed rapidly even in the presence of CCh (desensitization). Carbachol 84-87 transient receptor potential cation channel subfamily C member 5 Homo sapiens 0-5 15843439-3 2005 TRPC5 was initially activated by muscarinic stimulation using 100 microM carbachol (CCh) and then decayed rapidly even in the presence of CCh (desensitization). Carbachol 138-141 transient receptor potential cation channel subfamily C member 5 Homo sapiens 0-5 15843439-5 2005 The protein kinase C (PKC) inhibitors, 1 microM chelerythrine, 100 nM GF109203X, or PKC peptide inhibitor (19-36), inhibited this desensitization of TRPC5 activated by 100 microM CCh. Carbachol 179-182 transient receptor potential cation channel subfamily C member 5 Homo sapiens 149-154 15744749-1 2005 Carbachol (Cch), a muscarinic acetylcholine receptor (mAChR) agonist, increases intracellular-free Ca(2+) mobilization and induces mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) phosphorylation in MCF-7 human breast cancer cells. Carbachol 0-9 mitogen-activated protein kinase 1 Homo sapiens 208-211 15744749-1 2005 Carbachol (Cch), a muscarinic acetylcholine receptor (mAChR) agonist, increases intracellular-free Ca(2+) mobilization and induces mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) phosphorylation in MCF-7 human breast cancer cells. Carbachol 11-14 mitogen-activated protein kinase 1 Homo sapiens 208-211 15744749-3 2005 Phosphorylation of MAPK/ERK was mimicked by phorbol 12-myristate acetate (PMA), an activator of protein kinase C (PKC), but Cch-evoked MAPK/ERK activation was unaffected by down-regulation of PKC or by pretreatment of cells with GF109203X, a PKC inhibitor. Carbachol 124-127 mitogen-activated protein kinase 1 Homo sapiens 140-143 15744749-3 2005 Phosphorylation of MAPK/ERK was mimicked by phorbol 12-myristate acetate (PMA), an activator of protein kinase C (PKC), but Cch-evoked MAPK/ERK activation was unaffected by down-regulation of PKC or by pretreatment of cells with GF109203X, a PKC inhibitor. Carbachol 124-127 protein kinase C zeta Homo sapiens 192-195 15744749-3 2005 Phosphorylation of MAPK/ERK was mimicked by phorbol 12-myristate acetate (PMA), an activator of protein kinase C (PKC), but Cch-evoked MAPK/ERK activation was unaffected by down-regulation of PKC or by pretreatment of cells with GF109203X, a PKC inhibitor. Carbachol 124-127 protein kinase C zeta Homo sapiens 192-195 16099848-8 2005 In an ex vivo assay, we found that the Ca2+-dependent (carbachol-stimulated) glycoprotein secretion strongly inhibited by the calcium-activated chloride channel blocker niflumic acid (100 microm) was impaired in the distal colon of cftr-/- mice. Carbachol 55-64 cystic fibrosis transmembrane conductance regulator Mus musculus 232-236 15935407-5 2005 The nicotinic acetylcholine receptor antagonist, mecamylamine (5.0 and 10.0 microg), injected into the nucleus accumbens shell, which alone did not elicit any turning behaviour, significantly suppressed both the contraversive circling induced by carbachol (5.0 microg) and the contraversive pivoting induced by the mixture of SKF 38393 (5.0 microg) and quinpirole (10.0 microg). Carbachol 246-255 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 4-36 16083715-5 2005 RESULTS: EGF pretreatment of normal colonic mucosa inhibited ion transport responses to carbachol and forskolin but potentiated the reduced ion transport responses seen in dextran sulfate sodium (DSS)-treated and mdr1a knockout mouse colon. Carbachol 88-97 epidermal growth factor Mus musculus 9-12 15744749-4 2005 However, Cch-stimulated MAPK/ERK phosphorylation was completely blocked by myristoylated PKC-zeta pseudosubstrate, a specific inhibitor of PKC-zeta, and high doses of staurosporine. Carbachol 9-12 mitogen-activated protein kinase 1 Homo sapiens 29-32 15744749-4 2005 However, Cch-stimulated MAPK/ERK phosphorylation was completely blocked by myristoylated PKC-zeta pseudosubstrate, a specific inhibitor of PKC-zeta, and high doses of staurosporine. Carbachol 9-12 protein kinase C zeta Homo sapiens 89-97 15744749-4 2005 However, Cch-stimulated MAPK/ERK phosphorylation was completely blocked by myristoylated PKC-zeta pseudosubstrate, a specific inhibitor of PKC-zeta, and high doses of staurosporine. Carbachol 9-12 protein kinase C zeta Homo sapiens 139-147 15744749-5 2005 Pretreatment of human breast cancer cells with wortmannin or LY294002, selective inhibitors of phosphoinositide 3-kinase (PI3K), diminished Cch-mediated MAPK/ERK phosphorylation. Carbachol 140-143 mitogen-activated protein kinase 1 Homo sapiens 158-161 15894801-5 2005 Moreover, RyR1 and/or RyR3 in mouse airway smooth muscle also appear to mediate bronchoconstriction caused by the muscarinic receptor agonist carbachol. Carbachol 142-151 ryanodine receptor 1, skeletal muscle Mus musculus 10-14 15963958-6 2005 Microinjection of carbachol into the LSV caused an increase in firing rate of AHA angiotensin II-sensitive neurons. Carbachol 18-27 angiotensinogen Rattus norvegicus 82-96 15963958-7 2005 The carbachol-induced increase of firing rate of AHA angiotensin II-sensitive neurons was inhibited by pressure application of the excitatory amino acid receptor antagonist kynurenate but not by the AT1 receptor antagonist losartan onto the same neurons. Carbachol 4-13 angiotensinogen Rattus norvegicus 53-67 15963958-11 2005 It seems likely that the carbachol-induced activation of AHA angiotensin II-sensitive neurons is mainly mediated via excitatory amino acid receptors at AHA neurons. Carbachol 25-34 angiotensinogen Rattus norvegicus 61-75 15743890-3 2005 Secretagogues, including cholecystokinin (CCK) and the acetylcholine analog carbachol, increased the amount of GTP-bound RhoA and Rac1 and induced translocation from cytosol to a membrane fraction. Carbachol 76-85 ras homolog family member A Mus musculus 121-125 15743890-3 2005 Secretagogues, including cholecystokinin (CCK) and the acetylcholine analog carbachol, increased the amount of GTP-bound RhoA and Rac1 and induced translocation from cytosol to a membrane fraction. Carbachol 76-85 Rac family small GTPase 1 Mus musculus 130-134 15885660-8 2005 We demonstrate that calcium chelation or inhibition of calmodulin attenuates the effect of carbachol on AC6 activation. Carbachol 91-100 adenylate cyclase 6 Homo sapiens 104-107 15880140-8 2005 Atropine, propiverine and M-6 significantly shifted the cumulative concentration-response curves for carbachol (CCh) to higher concentrations. Carbachol 101-110 homeobox A7 Mus musculus 26-29 15880140-8 2005 Atropine, propiverine and M-6 significantly shifted the cumulative concentration-response curves for carbachol (CCh) to higher concentrations. Carbachol 112-115 homeobox A7 Mus musculus 26-29 15880140-10 2005 Propiverine, M-5 and M-14 reduced the maximum CCh effect, suggesting at least one additional mode of action. Carbachol 46-49 cholinergic receptor, muscarinic 5 Mus musculus 13-16 15955028-6 2005 Carbachol stimulation of M(3) and M(4) muscarinic AChR increased contractility, IPs accumulation, NOS activity and cGMP production. Carbachol 0-9 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 50-54 15955028-14 2005 The results obtained suggest that carbachol activation of M(3) and M(4) muscarinic AChRs, exerts a contractile effect on rat detrusor that is accompanied by an increased production of cGMP and nNOS activity. Carbachol 34-43 nitric oxide synthase 1 Rattus norvegicus 193-197 16012943-9 2005 Incubation of tissue with IL-4, IL-13, TGF-beta1, or PGE 2 enhanced carbachol-induced muscle cell contractility ( P < .05). Carbachol 68-77 interleukin 4 Mus musculus 26-30 16012943-9 2005 Incubation of tissue with IL-4, IL-13, TGF-beta1, or PGE 2 enhanced carbachol-induced muscle cell contractility ( P < .05). Carbachol 68-77 interleukin 13 Mus musculus 32-37 16012943-9 2005 Incubation of tissue with IL-4, IL-13, TGF-beta1, or PGE 2 enhanced carbachol-induced muscle cell contractility ( P < .05). Carbachol 68-77 transforming growth factor, beta 1 Mus musculus 39-48 15894801-5 2005 Moreover, RyR1 and/or RyR3 in mouse airway smooth muscle also appear to mediate bronchoconstriction caused by the muscarinic receptor agonist carbachol. Carbachol 142-151 ryanodine receptor 3 Mus musculus 22-26 15894801-6 2005 Inhibiting all RyR isoforms with > or = 200 microM ryanodine attenuated the graded carbachol-induced contractile responses of mouse bronchial rings and calcium responses of ASMCs throughout the range of carbachol used (50 nM to > or = 3 microM). Carbachol 86-95 ryanodine receptor 2, cardiac Mus musculus 15-18 15894801-6 2005 Inhibiting all RyR isoforms with > or = 200 microM ryanodine attenuated the graded carbachol-induced contractile responses of mouse bronchial rings and calcium responses of ASMCs throughout the range of carbachol used (50 nM to > or = 3 microM). Carbachol 206-215 ryanodine receptor 2, cardiac Mus musculus 15-18 15978011-8 2005 At network level, the effect of zolpidem (10 microm) on carbachol-induced oscillations in the CA3 area of gamma2I77/I77 mice was significantly different compared with controls. Carbachol 56-65 carbonic anhydrase 3 Mus musculus 94-97 15894015-3 2005 Carbachol (CCh) caused translocations of PKC-alpha, betaII, delta, and epsilon from the cytosol to the plasma membrane. Carbachol 0-9 protein kinase C, alpha Rattus norvegicus 41-50 15894015-3 2005 Carbachol (CCh) caused translocations of PKC-alpha, betaII, delta, and epsilon from the cytosol to the plasma membrane. Carbachol 11-14 protein kinase C, alpha Rattus norvegicus 41-50 15894015-5 2005 The CCh-stimulated insulin secretion was significantly suppressed by the generic PKC inhibitor chelerythrine. Carbachol 4-7 protein kinase C, alpha Rattus norvegicus 81-84 15894015-7 2005 These results suggest that the novel PKC isoforms activated by CCh, i.e., PKC-delta and/or epsilon, participate in the stimulatory effect of CCh on insulin secretion. Carbachol 63-66 protein kinase C, alpha Rattus norvegicus 37-40 15894015-7 2005 These results suggest that the novel PKC isoforms activated by CCh, i.e., PKC-delta and/or epsilon, participate in the stimulatory effect of CCh on insulin secretion. Carbachol 141-144 protein kinase C, alpha Rattus norvegicus 37-40 15779001-5 2005 The muscarinic agonist carbachol more efficiently promoted stress fiber formation and tyrosine phosphorylation of focal adhesion-associated proteins in M3 receptor-expressing cells adherent to fibronectin or collagen type I, as compared to polylysine. Carbachol 23-32 fibronectin 1 Homo sapiens 193-204 15779001-7 2005 However, total levels of MAPK and mitogen-activated protein kinase kinase (MEK) in the nucleus were significantly greater in cells adherent to ECM proteins for 2.5 h, and levels of activated MAPK and MEK in the nuclei of these cells were higher following carbachol stimulation, relative to levels in cells adherent to polylysine. Carbachol 255-264 mitogen-activated protein kinase kinase 7 Homo sapiens 75-78 15779001-7 2005 However, total levels of MAPK and mitogen-activated protein kinase kinase (MEK) in the nucleus were significantly greater in cells adherent to ECM proteins for 2.5 h, and levels of activated MAPK and MEK in the nuclei of these cells were higher following carbachol stimulation, relative to levels in cells adherent to polylysine. Carbachol 255-264 mitogen-activated protein kinase kinase 7 Homo sapiens 200-203 15855345-0 2005 Inhibitory effects of antipsychotics on carbachol-enhanced insulin secretion from perifused rat islets: role of muscarinic antagonism in antipsychotic-induced diabetes and hyperglycemia. Carbachol 40-49 insulin Homo sapiens 59-66 15855345-3 2005 At concentrations encompassing therapeutically relevant levels, olanzapine and clozapine reduced insulin secretion stimulated by 10 micromol/l carbachol plus 7 mmol/l glucose. Carbachol 143-152 insulin Homo sapiens 97-104 15855345-4 2005 This inhibition of insulin secretion was paralleled by significant reductions in carbachol-potentiated inositol phosphate accumulation. Carbachol 81-90 insulin Homo sapiens 19-26 15862177-5 2005 In the presence of both the CB1 antagonist AM251 (10(-6)m) and CP 55,940 (10(-5)m), the contractile response to carbachol reached 84+/-3% (n=6) of the original level. Carbachol 112-121 cannabinoid receptor 1 Homo sapiens 28-31 15760932-6 2005 Uncoupling the receptor-mediated activation of cytosolic phospholipase A(2) (cPLA(2)) with isotetrandrine reduces the activation of the ARC channels by carbachol and, correspondingly, markedly inhibits the [Ca(2+)](i) signals induced by low carbachol concentrations, whilst those signals seen at high agonist concentrations are essentially unaffected. Carbachol 152-161 phospholipase A2, group IVA (cytosolic, calcium-dependent) Mus musculus 47-84 15760932-6 2005 Uncoupling the receptor-mediated activation of cytosolic phospholipase A(2) (cPLA(2)) with isotetrandrine reduces the activation of the ARC channels by carbachol and, correspondingly, markedly inhibits the [Ca(2+)](i) signals induced by low carbachol concentrations, whilst those signals seen at high agonist concentrations are essentially unaffected. Carbachol 241-250 phospholipase A2, group IVA (cytosolic, calcium-dependent) Mus musculus 47-84 16079011-5 2005 Stimulation of M(1) and M(3) mAChR with carbachol caused an increase in vessel diameter, in iNOS-mRNA levels and in NOS activity in the retina. Carbachol 40-49 nitric oxide synthase 2 Rattus norvegicus 92-96 15857612-5 2005 Ca(2+) sensitization induced by phenylephrine, norepinephrine and carbachol was markedly antagonized by all three ROK inhibitors. Carbachol 66-75 Rho-associated coiled-coil containing protein kinase 2 Rattus norvegicus 114-117 15848807-6 2005 Electrophysiological study showed that carbachol excites almost one-third of orexin neurons and inhibits a small population of orexin neurons. Carbachol 39-48 hypocretin Mus musculus 77-83 15848807-6 2005 Electrophysiological study showed that carbachol excites almost one-third of orexin neurons and inhibits a small population of orexin neurons. Carbachol 39-48 hypocretin Mus musculus 127-133 15528258-5 2005 Preincubation of human cultured smooth muscle cells with IL-4 (P < 0.001) or IL-13 (P < 0.05) significantly enhanced carbachol-induced contraction, and this was significantly inhibited by the STAT6 inhibitor leflunomide (P < 0.0001). Carbachol 123-132 interleukin 4 Homo sapiens 57-61 15893601-0 2005 From synapse to gene product: prolonged expression of c-fos induced by a single microinjection of carbachol in the pontomesencephalic tegmentum. Carbachol 98-107 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 54-59 15893601-6 2005 These results demonstrate a sustained c-fos expression in response to pharmacological stimulation of the brain and suggest that carbachol"s acute effects induce LTPE via cholinergic receptors, with subsequent transsynaptic activation of the LDT/PPT maintaining the LTPE effect. Carbachol 128-137 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 38-43 15893601-6 2005 These results demonstrate a sustained c-fos expression in response to pharmacological stimulation of the brain and suggest that carbachol"s acute effects induce LTPE via cholinergic receptors, with subsequent transsynaptic activation of the LDT/PPT maintaining the LTPE effect. Carbachol 128-137 tachykinin precursor 1 Homo sapiens 245-248 15528258-5 2005 Preincubation of human cultured smooth muscle cells with IL-4 (P < 0.001) or IL-13 (P < 0.05) significantly enhanced carbachol-induced contraction, and this was significantly inhibited by the STAT6 inhibitor leflunomide (P < 0.0001). Carbachol 123-132 interleukin 13 Homo sapiens 80-85 15528258-5 2005 Preincubation of human cultured smooth muscle cells with IL-4 (P < 0.001) or IL-13 (P < 0.05) significantly enhanced carbachol-induced contraction, and this was significantly inhibited by the STAT6 inhibitor leflunomide (P < 0.0001). Carbachol 123-132 signal transducer and activator of transcription 6 Homo sapiens 198-203 15389641-1 2005 We have previously shown that the Gq protein coupled receptor (GqPCR) agonist, carbachol (CCh), transactivates and recruits epidermal growth factor receptor (EGFr)-dependent signaling mechanisms in intestinal epithelial cells. Carbachol 79-88 epidermal growth factor receptor Homo sapiens 124-156 15757506-4 2005 3 In diabetic carotid artery, the vasoconstrictor responses induced by noradrenaline (NE), endothelin-1 (ET-1), and angiotensin II (Ang II), were significantly increased whereas vasodilator responses to carbachol and histamine were significantly reduced. Carbachol 203-212 angiotensinogen Rattus norvegicus 132-138 15389641-8 2005 CCh also induced association of FAK with the EGFr and FAK phosphorylation was attenuated by an EGFr inhibitor, tyrphostin AG1478, and an inhibitor of Src family kinases, PP2. Carbachol 0-3 protein tyrosine kinase 2 Homo sapiens 32-35 15389641-1 2005 We have previously shown that the Gq protein coupled receptor (GqPCR) agonist, carbachol (CCh), transactivates and recruits epidermal growth factor receptor (EGFr)-dependent signaling mechanisms in intestinal epithelial cells. Carbachol 79-88 epidermal growth factor receptor Homo sapiens 158-162 15389641-8 2005 CCh also induced association of FAK with the EGFr and FAK phosphorylation was attenuated by an EGFr inhibitor, tyrphostin AG1478, and an inhibitor of Src family kinases, PP2. Carbachol 0-3 epidermal growth factor receptor Homo sapiens 45-49 15389641-1 2005 We have previously shown that the Gq protein coupled receptor (GqPCR) agonist, carbachol (CCh), transactivates and recruits epidermal growth factor receptor (EGFr)-dependent signaling mechanisms in intestinal epithelial cells. Carbachol 90-93 epidermal growth factor receptor Homo sapiens 124-156 15389641-8 2005 CCh also induced association of FAK with the EGFr and FAK phosphorylation was attenuated by an EGFr inhibitor, tyrphostin AG1478, and an inhibitor of Src family kinases, PP2. Carbachol 0-3 protein tyrosine kinase 2 Homo sapiens 54-57 15389641-1 2005 We have previously shown that the Gq protein coupled receptor (GqPCR) agonist, carbachol (CCh), transactivates and recruits epidermal growth factor receptor (EGFr)-dependent signaling mechanisms in intestinal epithelial cells. Carbachol 90-93 epidermal growth factor receptor Homo sapiens 158-162 15389641-8 2005 CCh also induced association of FAK with the EGFr and FAK phosphorylation was attenuated by an EGFr inhibitor, tyrphostin AG1478, and an inhibitor of Src family kinases, PP2. Carbachol 0-3 epidermal growth factor receptor Homo sapiens 95-99 15389641-3 2005 Therefore, the aim of the present study was to investigate if CCh stimulates activation of the focal adhesion-associated protein, focal adhesion kinase (FAK), in intestinal epithelia and, if so, to examine the signaling mechanisms involved. Carbachol 62-65 RNA 2',3'-cyclic phosphate and 5'-OH ligase Homo sapiens 95-128 15389641-8 2005 CCh also induced association of FAK with the EGFr and FAK phosphorylation was attenuated by an EGFr inhibitor, tyrphostin AG1478, and an inhibitor of Src family kinases, PP2. Carbachol 0-3 neuropeptide Y receptor Y6 (pseudogene) Homo sapiens 170-173 15389641-9 2005 The actin cytoskeleton disruptor, cytochalasin D (20 microM), abolished FAK phosphorylation in response to CCh but did not alter CCh-induced EGFr or ERK MAPK activation. Carbachol 107-110 protein tyrosine kinase 2 Homo sapiens 72-75 15389641-3 2005 Therefore, the aim of the present study was to investigate if CCh stimulates activation of the focal adhesion-associated protein, focal adhesion kinase (FAK), in intestinal epithelia and, if so, to examine the signaling mechanisms involved. Carbachol 62-65 protein tyrosine kinase 2 Homo sapiens 130-151 15389641-3 2005 Therefore, the aim of the present study was to investigate if CCh stimulates activation of the focal adhesion-associated protein, focal adhesion kinase (FAK), in intestinal epithelia and, if so, to examine the signaling mechanisms involved. Carbachol 62-65 protein tyrosine kinase 2 Homo sapiens 153-156 15389641-5 2005 CCh rapidly induced tyrosine phosphorylation of FAK in T84 cells. Carbachol 0-3 protein tyrosine kinase 2 Homo sapiens 48-51 15389641-7 2005 CCh-stimulated FAK phosphorylation was inhibited by a chelator of intracellular Ca2+, BAPTA/AM (20 microM), and was mimicked by thapsigargin (2 microM), which mobilizes intracellular Ca2+ in a receptor-independent fashion. Carbachol 0-3 protein tyrosine kinase 2 Homo sapiens 15-18 15762196-7 2005 DESIGN AND METHODS: Ionomycin- and carbamylcholine-stimulated H2O2 generation was measured in dog thyroid cells pretreated with the Clostridium difficile toxin B, which inhibits Rac proteins. Carbachol 35-50 Rac family small GTPase 1 Canis lupus familiaris 178-181 16245034-9 2005 The activation of apical Cl(-) conductance by carbachol was resistant against any blockers of the phospholipase C/IP3/protein kinase C pathway tested (e.g., U-73122, 2-ABP, Li(+), staurosporine), but was inhibited by the NO-synthase blocker L: -NNA. Carbachol 46-55 glutamate receptor interacting protein 2 Rattus norvegicus 168-171 15634940-1 2005 In airway smooth muscle cells, interleukin (IL)-4 inhibited both carbachol- and caffeine-induced calcium mobilization from the sarcoplasmic reticulum (SR). Carbachol 65-74 interleukin 4 Bos taurus 31-49 15634940-4 2005 Calcium transients in response to carbachol (10 microM) were significantly decreased to 0.34 +/- 0.10 of control after 20-min treatment with IL-4 but were 1.10 +/- 0.26 and 1.08 +/- 0.23 when wortmannin or deguelin, respectively, was added along with IL-4. Carbachol 34-43 interleukin 4 Bos taurus 141-145 15634940-4 2005 Calcium transients in response to carbachol (10 microM) were significantly decreased to 0.34 +/- 0.10 of control after 20-min treatment with IL-4 but were 1.10 +/- 0.26 and 1.08 +/- 0.23 when wortmannin or deguelin, respectively, was added along with IL-4. Carbachol 34-43 interleukin 4 Bos taurus 251-255 15814109-4 2005 Inhibitors of phospholipase A(2) (PLA(2)), COX and phospholipase C (PLC), calcium/calmodulin (CaM), NOS and soluble guanylate cyclase prevent the carbachol effect. Carbachol 146-155 phospholipase A2 group IB Rattus norvegicus 14-32 15647288-10 2005 Whereas whole-cell carbachol-induced TRPC3 current was blocked by 3 microM BTP2, single TRPC3 channel recordings revealed persistent short openings suggesting BTP2 reduces the open probability of the channel rather than its pore properties. Carbachol 19-28 transient receptor potential cation channel subfamily C member 3 Homo sapiens 37-42 15716122-3 2005 We found that carbachol-pretreatment enhanced both VIP- and forskolin-activated AC activities. Carbachol 14-23 vasoactive intestinal peptide Rattus norvegicus 51-54 15626773-3 2005 ATR exhibited significantly higher sensitivity to carbachol than CTR. Carbachol 50-59 ATR serine/threonine kinase Homo sapiens 0-3 15626773-4 2005 Pretreatment of ATR with E(2) shifted the carbachol concentration-response curve (CCRC) toward that of CTR. Carbachol 42-51 ATR serine/threonine kinase Homo sapiens 16-19 15721621-6 2005 Carbachol-stimulation of M1/M3 mAChR increased nNOS-mRNA levels associated with an increase of endogenous NO and cGMP production. Carbachol 0-9 nitric oxide synthase 1 Rattus norvegicus 47-51 15725947-7 2005 Third, the relaxant effect of carbachol was partially preserved in TAs of endothelial NOS deficient (eNOS-/-) animals and remained unchanged in the presence of indomethacin, indicating the involvement of an eNOS- and cyclooxygenase-independent mechanism in the mediation of the response. Carbachol 30-39 nitric oxide synthase 3, endothelial cell Mus musculus 74-89 15637297-6 2005 In contrast to WT, bradykinin- or carbachol-induced reduction in MVO2 was attenuated in nNOS-/-. Carbachol 34-43 nitric oxide synthase 1, neuronal Mus musculus 88-92 15707678-0 2005 High-affinity neurotensin receptor is involved in phosphoinositide hydrolysis stimulation by carbachol in neonatal rat brain. Carbachol 93-102 neurotensin Rattus norvegicus 14-25 15707678-5 2005 In 20-min incubation experiments, inositol phosphate accumulation by 10(-3) M carbachol was circa 240%, a value which attained 320-360% plus 10(-7) M neurotensin; this effect was totally blocked by 10(-7) M SR 48692. Carbachol 78-87 neurotensin Rattus norvegicus 150-161 15458922-13 2005 Stimulation of glands with carbachol, which elevates [Ca2+]i and activates PKC, induced apparent translocation of IQGAP1, but not IQGAP2, to apical poles of chief (zymogen) and mucous neck cells. Carbachol 27-36 IQ motif containing GTPase activating protein 2 Homo sapiens 130-136 15377496-8 2005 Western blot showed that carbachol, but not histamine, caused intense phosphorylation of extracellular signal-regulated kinase 1/2 and that LTD(4) significantly enhanced the phosphorylation induced by histamine, but not by carbachol. Carbachol 25-34 mitogen-activated protein kinase 3 Bos taurus 89-130 15987429-15 2005 Carbachol significantly increased VEGF expression in TMps, and this effect was totally reversed by methoctramine and pirenzepine. Carbachol 0-9 vascular endothelial growth factor A Mus musculus 34-38 16245207-5 2005 The results showed that the non hydrolysable agonist of mChR, carbachol (1 mM) together with GTP(g)S (100 microM) stimulated PARP-1 activity in the hippocampus by about 100%. Carbachol 62-71 melanin-concentrating hormone receptor 1 Mus musculus 56-60 16245207-5 2005 The results showed that the non hydrolysable agonist of mChR, carbachol (1 mM) together with GTP(g)S (100 microM) stimulated PARP-1 activity in the hippocampus by about 100%. Carbachol 62-71 poly(ADP-ribose) polymerase 1 Homo sapiens 125-131 16245207-6 2005 TMB-8, inhibitor of IP3 receptor decreased PARP-1 activation evoked by carbachol/GTP(g)S. Stimulation of mChR did not lead to free radicals generation but activate PARP-1 through IP3/Ca2+ regulated processes. Carbachol 71-80 inositol 1,4,5-trisphosphate receptor type 3 Homo sapiens 20-32 16245207-6 2005 TMB-8, inhibitor of IP3 receptor decreased PARP-1 activation evoked by carbachol/GTP(g)S. Stimulation of mChR did not lead to free radicals generation but activate PARP-1 through IP3/Ca2+ regulated processes. Carbachol 71-80 poly(ADP-ribose) polymerase 1 Homo sapiens 43-49 16245207-6 2005 TMB-8, inhibitor of IP3 receptor decreased PARP-1 activation evoked by carbachol/GTP(g)S. Stimulation of mChR did not lead to free radicals generation but activate PARP-1 through IP3/Ca2+ regulated processes. Carbachol 71-80 melanin-concentrating hormone receptor 1 Mus musculus 105-109 15649983-6 2005 Carbacholine (10(-4) M) significantly reduced tetanic force to 31 +/- 3% of controls, which underwent significant recovery upon application of Na+-K+ pump stimulators: salbutamol (10(-5) M), adrenaline (10(-5) M) and calcitonin gene-related peptide (CGRP; 10(-7) M). Carbachol 0-12 calcitonin-related polypeptide alpha Rattus norvegicus 217-248 15649983-6 2005 Carbacholine (10(-4) M) significantly reduced tetanic force to 31 +/- 3% of controls, which underwent significant recovery upon application of Na+-K+ pump stimulators: salbutamol (10(-5) M), adrenaline (10(-5) M) and calcitonin gene-related peptide (CGRP; 10(-7) M). Carbachol 0-12 calcitonin-related polypeptide alpha Rattus norvegicus 250-254 15721170-9 2005 These results strongly implicate 5-HT(1A) and 5-HT(2) receptors in the modulation of spectral power of carbachol-induced rhythmic activity and that 5-HT(1A) receptors are responsible for the prevailing effect of 5-HT. Carbachol 103-112 5-hydroxytryptamine receptor 1A Homo sapiens 33-40 15548524-10 2005 Interaction between PLD2 and tubulin was increased only after 1-2 min of carbachol stimulation when carbachol-stimulated PLD2 activity was decreased. Carbachol 73-82 phospholipase D2 Homo sapiens 20-24 15548524-10 2005 Interaction between PLD2 and tubulin was increased only after 1-2 min of carbachol stimulation when carbachol-stimulated PLD2 activity was decreased. Carbachol 100-109 phospholipase D2 Homo sapiens 20-24 15548524-10 2005 Interaction between PLD2 and tubulin was increased only after 1-2 min of carbachol stimulation when carbachol-stimulated PLD2 activity was decreased. Carbachol 100-109 phospholipase D2 Homo sapiens 121-125 15548524-11 2005 The expression of the tubulin binding region of PLD2 blocked the later decrease in carbachol-induced PLD activity by masking tubulin binding. Carbachol 83-92 phospholipase D2 Homo sapiens 48-52 15548524-11 2005 The expression of the tubulin binding region of PLD2 blocked the later decrease in carbachol-induced PLD activity by masking tubulin binding. Carbachol 83-92 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 48-51 15458922-13 2005 Stimulation of glands with carbachol, which elevates [Ca2+]i and activates PKC, induced apparent translocation of IQGAP1, but not IQGAP2, to apical poles of chief (zymogen) and mucous neck cells. Carbachol 27-36 IQ motif containing GTPase activating protein 1 Homo sapiens 114-120 15655501-4 2005 Genistein and daidzein antagonized the negative inotropic effect and the decrease in Ca(2+) transients induced by ET-1 by crosstalk with NE at high concentrations, but genistein did not affect the antiadrenergic effect of carbachol. Carbachol 222-231 endothelin 1 Canis lupus familiaris 114-118 15542611-8 2005 Activation of TRPC1 by thapsigargin or carbachol decreased MPP(+) neurotoxicity, which was partially dependent on external Ca(2+). Carbachol 39-48 transient receptor potential cation channel subfamily C member 1 Homo sapiens 14-19 15358594-8 2005 The pharmacological agonists phorbol 12-myristate 13-acetate and carbachol also led to the translocation of PKC-epsilon. Carbachol 65-74 protein kinase C epsilon Homo sapiens 108-119 15358594-10 2005 In response to carbachol, MARCKS translocates to the cytosol, indicating its phosphorylation, which is additionally confirmed biochemically. Carbachol 15-24 myristoylated alanine rich protein kinase C substrate Homo sapiens 26-32 15358594-11 2005 Consistent with this observation, carbachol induces the translocation of PKC-epsilon to proximity with MARCKS at the lateral membrane. Carbachol 34-43 protein kinase C epsilon Homo sapiens 73-84 15358594-11 2005 Consistent with this observation, carbachol induces the translocation of PKC-epsilon to proximity with MARCKS at the lateral membrane. Carbachol 34-43 myristoylated alanine rich protein kinase C substrate Homo sapiens 103-109 15762196-9 2005 RESULTS: Among the various agents inducing H2O2 generation in dog thyrocytes, carbamylcholine is the only one which activates Rac1, whereas phorbol ester and calcium increase alone have no effect, and cAMP inactivates it. Carbachol 78-93 Rac family small GTPase 1 Canis lupus familiaris 126-130 15637342-5 2005 We have evaluated changes in pancreatic amylase mRNA and total RNA after a single injection of carbachol and under fasting conditions. Carbachol 95-104 amylase 2a3 Rattus norvegicus 29-47 15998525-6 2005 At network level, 1-5 microM LY354740 significantly reduced the power of gamma frequency oscillations induced by 20 microM carbachol, 600 nM kainate and 5 mM K+ in hippocampal CA1 area. Carbachol 123-132 carbonic anhydrase 1 Homo sapiens 176-179 15617733-4 2005 It was found that activation of muscarinic acetylcholine receptors (mAChR) (1-10 microM carbachol), inhibited evoked responses across all layers of CA1. Carbachol 88-97 carbonic anhydrase 1 Homo sapiens 148-151 16125858-7 2005 In addition, patterns of tyrosine kinase receptor and Fos immunoreactivity similar to those observed in nerve growth factor-injected cats were present, in conjunction with long-lasting rapid eye movement sleep, following the microinjection of carbachol into the dorsal pons. Carbachol 243-252 Fos proto-oncogene, AP-1 transcription factor subunit Felis catus 54-57 16125858-7 2005 In addition, patterns of tyrosine kinase receptor and Fos immunoreactivity similar to those observed in nerve growth factor-injected cats were present, in conjunction with long-lasting rapid eye movement sleep, following the microinjection of carbachol into the dorsal pons. Carbachol 243-252 beta-nerve growth factor Felis catus 104-123 15636715-10 2005 While in carbachol treatment groups, the levels of TNF-alpha, Cr, ALT, CK-MB in plasma were decreased dramatically after enteral administration of carbachol during ischemia stage. Carbachol 9-18 tumor necrosis factor Oryctolagus cuniculus 51-60 15636715-10 2005 While in carbachol treatment groups, the levels of TNF-alpha, Cr, ALT, CK-MB in plasma were decreased dramatically after enteral administration of carbachol during ischemia stage. Carbachol 147-156 tumor necrosis factor Oryctolagus cuniculus 51-60 15485827-3 2004 The fragment is able to recognize its receptor on Chinese hamster ovary cells transfected with the M2 muscarinic acetylcholine receptor to block the effect of carbachol on this receptor and to exert an inverse agonist activity on the basal activity of the receptor. Carbachol 159-168 cholinergic receptor, muscarinic 2 Rattus norvegicus 99-135 15857717-6 2005 Compared with young, the power in the 20-80 Hz frequency range in area CA3 of slices from aged mice was reduced to 14% for kainate-induced oscillations and to 7% for carbachol-induced oscillations, whereas waveform, dominant frequency and coherence of the oscillation were unchanged. Carbachol 166-175 carbonic anhydrase 3 Mus musculus 71-74 16055947-6 2005 Pretreatment with the caspase inhibitor Boc-Asp-(OMe)-fluoromethylketone (BAF) plus neurotrophin-3 (NT-3) reduced C2- ceramide-induced CAD cell death, delaying cell death more effectively than either agent alone; and, most significantly, BAF and NT-3 enabled the cells remaining 24 h after toxin treatment to generate a normal metabolic response to the muscarinic agonist carbachol. Carbachol 372-381 neurotrophin 3 Homo sapiens 84-98 16055947-6 2005 Pretreatment with the caspase inhibitor Boc-Asp-(OMe)-fluoromethylketone (BAF) plus neurotrophin-3 (NT-3) reduced C2- ceramide-induced CAD cell death, delaying cell death more effectively than either agent alone; and, most significantly, BAF and NT-3 enabled the cells remaining 24 h after toxin treatment to generate a normal metabolic response to the muscarinic agonist carbachol. Carbachol 372-381 neurotrophin 3 Homo sapiens 100-104 15317664-5 2004 Whereas carbachol significantly upregulated myosin heavy chain SMA isoform expression in muscle strips held at slack length, carbachol did not significantly alter SMA expression in muscle strips at sinusoidal length oscillation. Carbachol 8-17 myosin heavy chain 11 Bos taurus 44-66 15317664-5 2004 Whereas carbachol significantly upregulated myosin heavy chain SMA isoform expression in muscle strips held at slack length, carbachol did not significantly alter SMA expression in muscle strips at sinusoidal length oscillation. Carbachol 8-17 survival of motor neuron 1, telomeric Bos taurus 63-66 15317664-6 2004 Carbachol also significantly upregulated GAPDH expression in bovine tracheal smooth muscle. Carbachol 0-9 glyceraldehyde-3-phosphate dehydrogenase Bos taurus 41-46 15317664-9 2004 U0126 (10 muM) completely inhibited carbachol-induced ERK1/2 MAPK phosphorylation but did not significantly affect carbachol-induced upregulation of GAPDH and SMA expression, suggesting that the ERK1/2 MAPK pathway was not the underlying mechanism. Carbachol 36-45 mitogen-activated protein kinase 3 Bos taurus 54-60 15317664-9 2004 U0126 (10 muM) completely inhibited carbachol-induced ERK1/2 MAPK phosphorylation but did not significantly affect carbachol-induced upregulation of GAPDH and SMA expression, suggesting that the ERK1/2 MAPK pathway was not the underlying mechanism. Carbachol 36-45 mitogen-activated protein kinase 1 Bos taurus 61-65 15317664-9 2004 U0126 (10 muM) completely inhibited carbachol-induced ERK1/2 MAPK phosphorylation but did not significantly affect carbachol-induced upregulation of GAPDH and SMA expression, suggesting that the ERK1/2 MAPK pathway was not the underlying mechanism. Carbachol 36-45 mitogen-activated protein kinase 3 Bos taurus 195-201 15317664-9 2004 U0126 (10 muM) completely inhibited carbachol-induced ERK1/2 MAPK phosphorylation but did not significantly affect carbachol-induced upregulation of GAPDH and SMA expression, suggesting that the ERK1/2 MAPK pathway was not the underlying mechanism. Carbachol 36-45 mitogen-activated protein kinase 1 Bos taurus 202-206 15588249-7 2004 UII (1 nmol/l) also inhibited the insulin responses induced by carbachol, glucagon-like peptide-1, and a calcium channel agonist (BAY K 8644). Carbachol 63-72 urotensin 2 Rattus norvegicus 0-3 15579537-2 2004 With nystatin-perforated recording, the magnitude and time courses of activation and inactivation of carbachol (CCh; 100 microM)-activated TRPC6 currents (I(TRPC6)) were enhanced and accelerated, respectively, by extracellular Ca2+ (Ca2+(o)) whether it was continuously present or applied after receptor stimulation. Carbachol 101-110 transient receptor potential cation channel subfamily C member 6 Homo sapiens 139-144 15579537-2 2004 With nystatin-perforated recording, the magnitude and time courses of activation and inactivation of carbachol (CCh; 100 microM)-activated TRPC6 currents (I(TRPC6)) were enhanced and accelerated, respectively, by extracellular Ca2+ (Ca2+(o)) whether it was continuously present or applied after receptor stimulation. Carbachol 101-110 transient receptor potential cation channel subfamily C member 6 Homo sapiens 157-162 15579537-2 2004 With nystatin-perforated recording, the magnitude and time courses of activation and inactivation of carbachol (CCh; 100 microM)-activated TRPC6 currents (I(TRPC6)) were enhanced and accelerated, respectively, by extracellular Ca2+ (Ca2+(o)) whether it was continuously present or applied after receptor stimulation. Carbachol 112-115 transient receptor potential cation channel subfamily C member 6 Homo sapiens 139-144 15579537-2 2004 With nystatin-perforated recording, the magnitude and time courses of activation and inactivation of carbachol (CCh; 100 microM)-activated TRPC6 currents (I(TRPC6)) were enhanced and accelerated, respectively, by extracellular Ca2+ (Ca2+(o)) whether it was continuously present or applied after receptor stimulation. Carbachol 112-115 transient receptor potential cation channel subfamily C member 6 Homo sapiens 157-162 15579537-8 2004 Instead, single CCh-activated TRPC7 channel activity was concentration-dependently suppressed by nanomolar Ca2+(i) via CaM and conversely enhanced by IP3. Carbachol 16-19 transient receptor potential cation channel subfamily C member 7 Homo sapiens 30-35 15613736-3 2004 The muscarinic agent - carbachol-induced HPA response was considerably supressed by piroxicam, a predominantly constitutive cyclooxygenase (COX-1) inhibitor and significantly diminished by indomethacin, a non-selective COX blocker, but was unaffected by compound NS-398, an inducible cyclooxygenase (COX-2) antagonist. Carbachol 23-32 mitochondrially encoded cytochrome c oxidase I Homo sapiens 140-145 15518913-1 2004 A novel VIP derivative, [R15, 20, 21, L17]-VIP-GRR (IK312532), relaxed potently the carbachol-induced contraction of guinea-pig isolated trachea with longer duration than that induced by VIP. Carbachol 84-93 vasoactive intestinal peptide Rattus norvegicus 8-11 15518913-1 2004 A novel VIP derivative, [R15, 20, 21, L17]-VIP-GRR (IK312532), relaxed potently the carbachol-induced contraction of guinea-pig isolated trachea with longer duration than that induced by VIP. Carbachol 84-93 vasoactive intestinal peptide Rattus norvegicus 43-46 15518913-1 2004 A novel VIP derivative, [R15, 20, 21, L17]-VIP-GRR (IK312532), relaxed potently the carbachol-induced contraction of guinea-pig isolated trachea with longer duration than that induced by VIP. Carbachol 84-93 VIP peptides Cavia porcellus 43-46 15380628-6 2004 A 60-75% decline in contractility was observed when Cav1 knockout muscle strips were stimulated with electric current or carbachol, compared to wildtype muscle strips. Carbachol 121-130 caveolin 1, caveolae protein Mus musculus 52-56 15742997-7 2004 The effects of ghrelin on spontaneous contractile activities of isolated strips from stomach and jejunum were also investigated and the influence of ghrelin on motor responses to carbachol and electrical field stimulation was examined. Carbachol 179-188 ghrelin and obestatin prepropeptide Rattus norvegicus 149-156 15269004-5 2004 Carbachol induced ERK1/2 phosphorylation by 300% of basal value. Carbachol 0-9 mitogen-activated protein kinase 3 Bos taurus 18-24 15269004-6 2004 U0126 (10 microM) completely inhibited carbachol-induced ERK1/2 phosphorylation but did not significantly affect the correlation between alpha-actinin P/S and carbachol concentration. Carbachol 39-48 mitogen-activated protein kinase 3 Bos taurus 57-63 15613736-3 2004 The muscarinic agent - carbachol-induced HPA response was considerably supressed by piroxicam, a predominantly constitutive cyclooxygenase (COX-1) inhibitor and significantly diminished by indomethacin, a non-selective COX blocker, but was unaffected by compound NS-398, an inducible cyclooxygenase (COX-2) antagonist. Carbachol 23-32 mitochondrially encoded cytochrome c oxidase II Homo sapiens 300-305 15500825-6 2004 Overnight stimulation of primary cultured rabbit lacrimal gland acinar cells with 10 microM carbachol (CCh) significantly decreased the abundance of mature cathepsin B in the pre-lysosome and lysosome; decreased the abundance of preprocathepsin B in fractions containing the TGN and late endosome; increased the abundance of procathepsin B in fractions containing the basal-lateral membrane; and increased the accumulation of endocytosed [(125)I]-EGF in the recycling endosome. Carbachol 92-101 cathepsin B Oryctolagus cuniculus 156-167 15500825-6 2004 Overnight stimulation of primary cultured rabbit lacrimal gland acinar cells with 10 microM carbachol (CCh) significantly decreased the abundance of mature cathepsin B in the pre-lysosome and lysosome; decreased the abundance of preprocathepsin B in fractions containing the TGN and late endosome; increased the abundance of procathepsin B in fractions containing the basal-lateral membrane; and increased the accumulation of endocytosed [(125)I]-EGF in the recycling endosome. Carbachol 103-106 cathepsin B Oryctolagus cuniculus 156-167 15485492-7 2004 Moreover, cholinergic stimulation of neuroblastoma cells by carbachol, which activated endogenous Ras/ERK1/2, induced a significant increase in ROS levels and elicited membrane translocation of p67(phox) and Rac. Carbachol 60-69 mitogen-activated protein kinase 3 Homo sapiens 102-108 15485492-7 2004 Moreover, cholinergic stimulation of neuroblastoma cells by carbachol, which activated endogenous Ras/ERK1/2, induced a significant increase in ROS levels and elicited membrane translocation of p67(phox) and Rac. Carbachol 60-69 CD33 molecule Homo sapiens 194-197 15485492-7 2004 Moreover, cholinergic stimulation of neuroblastoma cells by carbachol, which activated endogenous Ras/ERK1/2, induced a significant increase in ROS levels and elicited membrane translocation of p67(phox) and Rac. Carbachol 60-69 AKT serine/threonine kinase 1 Homo sapiens 208-211 15485492-8 2004 ROS generation induced by carbachol required the activation of ERK1/2 and PI3K. Carbachol 26-35 mitogen-activated protein kinase 3 Homo sapiens 63-69 15502635-5 2004 Glucose-induced Crk-p130Cas association was rapid and sustained and was maximal with the combination of glucose and carbachol, paralleling insulin secretion. Carbachol 116-125 CRK proto-oncogene, adaptor protein Rattus norvegicus 16-19 15514253-9 2004 In colon, there was a change of sensitivity (50% effective concentration) to angiotensin II in WKY (P < 0.05) due to increased SF and a change of sensitivity to prostaglandin (PG)E(2) and carbachol in SHR (P < 0.05). Carbachol 191-200 angiotensinogen Rattus norvegicus 77-91 15502635-5 2004 Glucose-induced Crk-p130Cas association was rapid and sustained and was maximal with the combination of glucose and carbachol, paralleling insulin secretion. Carbachol 116-125 BCAR1 scaffold protein, Cas family member Rattus norvegicus 20-27 15467192-0 2004 Carbachol-induced membrane ruffling and its desensitization in rat basophilic leukemia (RBL-2H3) cells transfected with m3 muscarinic acetylcholine receptors. Carbachol 0-9 cholinergic receptor muscarinic 3 Homo sapiens 120-157 15483626-5 2004 Carbachol- and phenylephrine-contracted smooth muscle strips from colon and aorta, respectively, of IRAG(Delta12/Delta12) mice were not relaxed by cGMP, while cAMP-mediated relaxation was unperturbed. Carbachol 0-9 inositol 1,4,5-triphosphate receptor associated 1 Mus musculus 100-104 15217780-1 2004 PKC is known to be activated by pancreatic secretagogues such as CCK and carbachol and to participate along with calcium in amylase release. Carbachol 73-82 protein kinase C, alpha Rattus norvegicus 0-3 15371786-11 2004 Similarly, bladders from SMalphaA-null mice generated less force than wild-type mice in response to pretreatment EFS, and EFS after carbachol and atropine, although the difference was not significant. Carbachol 132-141 actin alpha 2, smooth muscle, aorta Mus musculus 25-33 15277524-1 2004 We have seen that protein kinase Calpha (PKCalpha) is transiently translocated to the plasma membrane by carbachol stimulation of neuroblastoma cells. Carbachol 105-114 protein kinase C alpha Homo sapiens 18-39 15190096-5 2004 In contrast, just two suprathreshold stimuli >50 Hz triggered a prominent sAHP sensitive to bath-applications of isoproterenol, carbachol, or intracellularly applied BAPTA, suggesting that the underlying current is the Ca2+-activated K+ current, the sIAHP. Carbachol 131-140 carbonic anhydrase 2 Rattus norvegicus 222-225 15277524-1 2004 We have seen that protein kinase Calpha (PKCalpha) is transiently translocated to the plasma membrane by carbachol stimulation of neuroblastoma cells. Carbachol 105-114 protein kinase C alpha Homo sapiens 41-49 15277524-6 2004 Carbachol stimulation induced a transient translocation of PKCalpha to the plasma membrane and a sustained translocation of kinase-dead PKCalpha. Carbachol 0-9 protein kinase C alpha Homo sapiens 59-67 15277524-6 2004 Carbachol stimulation induced a transient translocation of PKCalpha to the plasma membrane and a sustained translocation of kinase-dead PKCalpha. Carbachol 0-9 protein kinase C alpha Homo sapiens 136-144 15275856-8 2004 Amylase release stimulated by carbachol and GSNO was inhibited by addition of the sGC inhibitor, ODQ, and cGMP-dependent protein kinase inhibitor, KT-5823. Carbachol 30-39 guanylate cyclase 1 soluble subunit alpha 1 Rattus norvegicus 82-85 15359086-1 2004 Carbachol (CCh) caused a dose-dependent release of beta-hexosaminidase and an increase in the production of inositol 1,4,5-trisphosphate (IP3) in RBL-2H3 cells transfected with m2 mAChR cDNA (RBL-m2 cells). Carbachol 0-9 O-GlcNAcase Rattus norvegicus 51-70 15359086-1 2004 Carbachol (CCh) caused a dose-dependent release of beta-hexosaminidase and an increase in the production of inositol 1,4,5-trisphosphate (IP3) in RBL-2H3 cells transfected with m2 mAChR cDNA (RBL-m2 cells). Carbachol 11-14 O-GlcNAcase Rattus norvegicus 51-70 15322258-5 2004 These AChE inhibitors had no significant effect on either the amplitude or kinetics of alpha7 nAChRs activated by ACh, but they slowed the rate of recovery from desensitization through an indirect mechanism; responses activated with either choline or carbachol were unaffected. Carbachol 251-260 acetylcholinesterase Rattus norvegicus 6-10 15272012-6 2004 In 1321N1 human astrocytoma cells, the endogenous PDE1 (identified as PDE1A by reverse transcriptase-PCR) was largely insensitive to Ca2+ released from carbachol-sensitive stores but was robustly stimulated by a similar rise in [Ca2+]i due to carbachol-induced Ca2+ influx. Carbachol 152-161 phosphodiesterase 1A Homo sapiens 70-75 15272012-6 2004 In 1321N1 human astrocytoma cells, the endogenous PDE1 (identified as PDE1A by reverse transcriptase-PCR) was largely insensitive to Ca2+ released from carbachol-sensitive stores but was robustly stimulated by a similar rise in [Ca2+]i due to carbachol-induced Ca2+ influx. Carbachol 243-252 phosphodiesterase 1A Homo sapiens 70-75 15279909-7 2004 We also found a marked decrease on Cch-stimulated Ca2+ mobilization in pretreated FRT cells with forskolin, an activator of protein kinase A (PKA), but the preincubation of cells with genistein, an inhibitor of protein tyrosine kinases, had no effect on Ca2+ mobilization induced by Cch. Carbachol 35-38 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 124-140 15279909-7 2004 We also found a marked decrease on Cch-stimulated Ca2+ mobilization in pretreated FRT cells with forskolin, an activator of protein kinase A (PKA), but the preincubation of cells with genistein, an inhibitor of protein tyrosine kinases, had no effect on Ca2+ mobilization induced by Cch. Carbachol 35-38 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 142-145 15327778-8 2004 Importantly, carbachol, not thapsigargin, increased surface expression of TRPC3 that was attenuated by TeNT and not by BAPTA. Carbachol 13-22 transient receptor potential cation channel subfamily C member 3 Homo sapiens 74-79 15327778-9 2004 In aggregate, these data suggest that VAMP2-dependent exocytosis regulates plasma membrane insertion of TRPC3 channels and contributes to carbachol-stimulation of Ca2+ influx. Carbachol 138-147 vesicle associated membrane protein 2 Homo sapiens 38-43 15264218-3 2004 Histamine, glutamate, carbachol, serotonin or gamma-aminobutyric acid (GABA) caused an increase of FGF-1 content. Carbachol 22-31 fibroblast growth factor 1 Rattus norvegicus 99-104 15287742-8 2004 BC3H1 cells containing a fetal mouse muscle-type nAChR were used, and the receptor was activated by carbamoylcholine. Carbachol 100-116 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 49-54 15287742-11 2004 Inhibitors and compounds that alleviate inhibition were tested by determining their effects on the whole-cell current due to activation of the nAChR by carbamoylcholine. Carbachol 152-168 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 143-148 15242775-3 2004 Overexpression of Bcl-2 inhibited carbachol-induced ROCK-I cleavage, indicating a mitochondrial apoptotic pathway. Carbachol 34-43 BCL2 apoptosis regulator Homo sapiens 18-23 15242775-3 2004 Overexpression of Bcl-2 inhibited carbachol-induced ROCK-I cleavage, indicating a mitochondrial apoptotic pathway. Carbachol 34-43 Rho associated coiled-coil containing protein kinase 1 Homo sapiens 52-58 15242775-5 2004 Insulin, a major survival factor in many cells, strongly increased phosphorylation of Akt, which was completely blocked by carbachol. Carbachol 123-132 insulin Homo sapiens 0-7 15242775-5 2004 Insulin, a major survival factor in many cells, strongly increased phosphorylation of Akt, which was completely blocked by carbachol. Carbachol 123-132 AKT serine/threonine kinase 1 Homo sapiens 86-89 15242775-7 2004 In parallel with these observations, carbachol attenuated insulin-stimulated tyrosine phosphorylation of insulin receptor substrate-1, an effect eliminated by orthovanadate. Carbachol 37-46 insulin Homo sapiens 58-65 15242775-7 2004 In parallel with these observations, carbachol attenuated insulin-stimulated tyrosine phosphorylation of insulin receptor substrate-1, an effect eliminated by orthovanadate. Carbachol 37-46 insulin Homo sapiens 105-112 15242775-8 2004 On the other hand, carbachol induced rapid stimulation of endogenous RhoA, and expression of a constitutively active mutant of RhoA increased ROCK-I cleavage. Carbachol 19-28 ras homolog family member A Homo sapiens 69-73 15242775-8 2004 On the other hand, carbachol induced rapid stimulation of endogenous RhoA, and expression of a constitutively active mutant of RhoA increased ROCK-I cleavage. Carbachol 19-28 ras homolog family member A Homo sapiens 127-131 15242775-8 2004 On the other hand, carbachol induced rapid stimulation of endogenous RhoA, and expression of a constitutively active mutant of RhoA increased ROCK-I cleavage. Carbachol 19-28 Rho associated coiled-coil containing protein kinase 1 Homo sapiens 142-148 15242775-9 2004 Orthovanadate and the dominant negative mutant of RhoA partially, and their combination completely, inhibited carbachol-induced ROCK-I cleavage and apoptosis. Carbachol 110-119 ras homolog family member A Homo sapiens 50-54 15242775-9 2004 Orthovanadate and the dominant negative mutant of RhoA partially, and their combination completely, inhibited carbachol-induced ROCK-I cleavage and apoptosis. Carbachol 110-119 Rho associated coiled-coil containing protein kinase 1 Homo sapiens 128-134 15286428-2 2004 Among the CHO-H1/M1, CHO-H1/M3, and CHO-H1/M5 cells, carbachol treatment of the CHO-H1/M3 cells time-dependently led to remarkable down-regulation of the H1R to 60% of the control level. Carbachol 53-62 histamine receptor H1 Homo sapiens 154-157 15286428-4 2004 Stimulation of CHO-H1/M5 cells by carbachol induced significant but only small H1R down-regulation. Carbachol 34-43 histamine receptor H1 Homo sapiens 79-82 15286428-6 2004 H1R-mediated accumulation of inositol phosphates in CHO-H1/M3 cells with long-term expose to carbachol was decreased to 60% compared with non-treated cells. Carbachol 93-102 histamine receptor H1 Homo sapiens 0-3 15233804-5 2004 Treatment of PKCalpha-deficient cells with carbachol induced a significant release of sAPPalpha. Carbachol 43-52 protein kinase C alpha Homo sapiens 13-21 15233804-7 2004 The response to carbachol is instead completely blocked in PKCepsilon-deficient cells suggesting the importance of PKCepsilon in coupling cholinergic receptors with APP metabolism. Carbachol 16-25 protein kinase C epsilon Homo sapiens 59-69 15087463-4 2004 Cellular PLD activity was increased in response to a variety of secretagogues including the nutrient glucose and the cholinergic receptor agonist carbamoylcholine. Carbachol 146-162 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 9-12 15182196-3 2004 In co-immunoprecipitation studies, both glutamate and carbachol increased the association of GRK2 with mGluR1a. Carbachol 54-63 G protein-coupled receptor kinase 2 Homo sapiens 93-97 15182196-4 2004 Co-addition of the protein kinase C (PKC) inhibitor GF109203X and the Ca(2+) calmodulin-dependent kinase II (CaMKII) inhibitor KN-93 blocked the ability of glutamate and carbachol to increase the association of GRK2 with mGluR1a. Carbachol 170-179 calcium/calmodulin dependent protein kinase II gamma Homo sapiens 109-115 15182196-8 2004 The carbachol-induced heterologous desensitization and internalization of mGluR1a was blocked by LY367385, an mGluR1a antagonist with inverse agonist activity. Carbachol 4-13 glutamate metabotropic receptor 1 Homo sapiens 74-80 15182196-8 2004 The carbachol-induced heterologous desensitization and internalization of mGluR1a was blocked by LY367385, an mGluR1a antagonist with inverse agonist activity. Carbachol 4-13 glutamate metabotropic receptor 1 Homo sapiens 110-116 15272012-7 2004 Gd3+, which effectively blocked thapsigargin-induced CCE and its effect on PDE1A, also inhibited the activation of PDE1A by carbachol-induced Ca2+ entry. Carbachol 124-133 phosphodiesterase 1A Homo sapiens 115-120 15255942-0 2004 Role of phospholipase D signaling in ethanol-induced inhibition of carbachol-stimulated DNA synthesis of 1321N1 astrocytoma cells. Carbachol 67-76 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 8-23 15255942-4 2004 1-Butanol, which is a substrate for PLD and inhibits PA formation, inhibited carbachol-induced cell proliferation and the underlying intracellular signaling, whereas its analog tert-butanol, which is a poor substrate for PLD, was much less effective. Carbachol 77-86 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 36-39 15255942-7 2004 Taken together, these results indicate that PLD activation plays an important role in carbachol-induced astroglial cell proliferation by generating the second messenger PA, which activates PKC zeta. Carbachol 86-95 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 44-47 15255942-7 2004 Taken together, these results indicate that PLD activation plays an important role in carbachol-induced astroglial cell proliferation by generating the second messenger PA, which activates PKC zeta. Carbachol 86-95 protein kinase C zeta Homo sapiens 189-197 15255942-8 2004 Moreover, the effect of ethanol on carbachol-induced proliferation appears to be mediated, at least in part, by its ability to interact with PLD leading to a decreased synthesis of PA. Carbachol 35-44 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 141-144 15247775-9 2004 CONCLUSIONS: These results indicate that in human UBSM CCh induces contraction, not only by increasing [Ca2+]i, but also by increasing the Ca2+ sensitivity of the contractile apparatus in a ROCK and protein kinase C dependent manner. Carbachol 55-58 Rho associated coiled-coil containing protein kinase 1 Homo sapiens 190-194 15150258-4 2004 However, activation of ERK by epidermal growth factor or carbachol were not suppressed by inhibition of CaMKK, indicating specificity for this "cross-talk." Carbachol 57-66 mitogen-activated protein kinase 1 Homo sapiens 23-26 15029229-7 2004 In acini exposed to replication-defective or UV-inactivated Ad, carbachol-stimulated release of bulk protein and beta-hexosaminidase were significantly (P< or =0.05) inhibited to an extent proportional to the loss of rab3D-enriched mature secretory vesicles associated with these treatments. Carbachol 64-73 ras-related protein Rab-3D Oryctolagus cuniculus 220-225 15155843-4 2004 The heterologous internalization of the mGluR1 splice variants triggered by carbachol was also inhibited by isoprenaline and forskolin in a PKA-sensitive fashion, whereas the constitutive (agonist-independent) internalization of mGluR1a was inhibited only modestly by PKA activation. Carbachol 76-85 glutamate metabotropic receptor 1 Homo sapiens 40-46 15023993-2 2004 Whole cell membrane currents with distinctive inward and outward rectification were activated by carbachol (CCh) in TRPC6-expressing cells, but not in lacZ-transfected controls. Carbachol 97-106 transient receptor potential cation channel subfamily C member 6 Homo sapiens 116-121 15182196-9 2004 Furthermore, LY367385 blocked the ability of carbachol to increase the association of GRK2 with mGluR1a. Carbachol 45-54 G protein-coupled receptor kinase 2 Homo sapiens 86-90 15023993-2 2004 Whole cell membrane currents with distinctive inward and outward rectification were activated by carbachol (CCh) in TRPC6-expressing cells, but not in lacZ-transfected controls. Carbachol 108-111 transient receptor potential cation channel subfamily C member 6 Homo sapiens 116-121 15023993-9 2004 Surprisingly, concentrations of CCh that produced little or no response in the absence of OAG, produced increases in TRPC6 currents in the presence of OAG that were larger than the sum of either agent alone. Carbachol 32-35 transient receptor potential cation channel subfamily C member 6 Homo sapiens 117-122 15023993-12 2004 This synergy may explain, at least in part, the steep dose-response relationship observed for CCh-induced TRPC6 currents expressed in HEK cells. Carbachol 94-97 transient receptor potential cation channel subfamily C member 6 Homo sapiens 106-111 15059931-7 2004 Treatment with TGF-beta also induces a contractile phenotype that responds to the muscarinic agonist carbachol and is not immediately reversed on TGF-beta withdrawal. Carbachol 101-110 transforming growth factor beta 1 Homo sapiens 15-23 15086448-5 2004 The depolarization evoked by carbachol (50 microm) was potentiated from 5.2 +/- 0.7 mV (n = 6) in control neurones to 27.5 +/- 2.0 mV (n = 10) in TNF-alpha-treated cells. Carbachol 29-38 tumor necrosis factor Rattus norvegicus 146-155 14978207-0 2004 Carbachol regulation of rabbit ileal brush border Na+-H+ exchanger 3 (NHE3) occurs through changes in NHE3 trafficking and complex formation and is Src dependent. Carbachol 0-9 sodium/hydrogen exchanger 3 Oryctolagus cuniculus 50-68 14769130-4 2004 However, the effects of carbachol were prevented by sodium vanadate, a protein tyrosine phosphatase inhibitor, and were accompanied by reduced insulin-stimulated IRS-1 tyrosine phosphorylation and recruitment of the 85 kDa regulatory subunit of PI3K to IRS-1, but not by reduced IGF-1 receptor kinase activity. Carbachol 24-33 insulin receptor substrate 1 Homo sapiens 162-167 14769130-4 2004 However, the effects of carbachol were prevented by sodium vanadate, a protein tyrosine phosphatase inhibitor, and were accompanied by reduced insulin-stimulated IRS-1 tyrosine phosphorylation and recruitment of the 85 kDa regulatory subunit of PI3K to IRS-1, but not by reduced IGF-1 receptor kinase activity. Carbachol 24-33 insulin receptor substrate 1 Homo sapiens 253-258 14769130-4 2004 However, the effects of carbachol were prevented by sodium vanadate, a protein tyrosine phosphatase inhibitor, and were accompanied by reduced insulin-stimulated IRS-1 tyrosine phosphorylation and recruitment of the 85 kDa regulatory subunit of PI3K to IRS-1, but not by reduced IGF-1 receptor kinase activity. Carbachol 24-33 insulin like growth factor 1 Homo sapiens 279-284 14769130-5 2004 The inhibitory effect of carbachol was reproduced by okadaic acid, a protein serine/threonine phosphatase inhibitor, but not by PDGF, yet all three agents stimulated the serine phosphorylation of IRS-1 at residues Ser312, Ser616 and Ser636/639, albeit to different extents. Carbachol 25-34 insulin receptor substrate 1 Homo sapiens 196-201 14978207-11 2004 Moreover, Src was involved in the carbachol-mediated inhibition since: (1) c-Src was rapidly activated in the detergent-resistant membranes by carbachol; and (2) carbachol inhibition of ileal Na(+) absorption was completely abolished by the Src family inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2). Carbachol 143-152 proto-oncogene tyrosine-protein kinase Src Oryctolagus cuniculus 77-80 14978207-11 2004 Moreover, Src was involved in the carbachol-mediated inhibition since: (1) c-Src was rapidly activated in the detergent-resistant membranes by carbachol; and (2) carbachol inhibition of ileal Na(+) absorption was completely abolished by the Src family inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2). Carbachol 143-152 proto-oncogene tyrosine-protein kinase Src Oryctolagus cuniculus 10-13 14978207-11 2004 Moreover, Src was involved in the carbachol-mediated inhibition since: (1) c-Src was rapidly activated in the detergent-resistant membranes by carbachol; and (2) carbachol inhibition of ileal Na(+) absorption was completely abolished by the Src family inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2). Carbachol 143-152 proto-oncogene tyrosine-protein kinase Src Oryctolagus cuniculus 77-80 14978207-11 2004 Moreover, Src was involved in the carbachol-mediated inhibition since: (1) c-Src was rapidly activated in the detergent-resistant membranes by carbachol; and (2) carbachol inhibition of ileal Na(+) absorption was completely abolished by the Src family inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2). Carbachol 143-152 proto-oncogene tyrosine-protein kinase Src Oryctolagus cuniculus 77-80 14978207-0 2004 Carbachol regulation of rabbit ileal brush border Na+-H+ exchanger 3 (NHE3) occurs through changes in NHE3 trafficking and complex formation and is Src dependent. Carbachol 0-9 sodium/hydrogen exchanger 3 Oryctolagus cuniculus 70-74 14978207-0 2004 Carbachol regulation of rabbit ileal brush border Na+-H+ exchanger 3 (NHE3) occurs through changes in NHE3 trafficking and complex formation and is Src dependent. Carbachol 0-9 sodium/hydrogen exchanger 3 Oryctolagus cuniculus 102-106 14978207-0 2004 Carbachol regulation of rabbit ileal brush border Na+-H+ exchanger 3 (NHE3) occurs through changes in NHE3 trafficking and complex formation and is Src dependent. Carbachol 0-9 proto-oncogene tyrosine-protein kinase Src Oryctolagus cuniculus 148-151 14978207-12 2004 Moreover, the carbachol-induced increase in the size of NHE3-containing complexes was reversed by PP2. Carbachol 14-23 sodium/hydrogen exchanger 3 Oryctolagus cuniculus 56-60 14978207-2 2004 We have previously shown that ileal BBM NHE3 activity is rapidly inhibited by carbachol, an agonist that mimics cholinergic activation in digestion. Carbachol 78-87 sodium/hydrogen exchanger 3 Oryctolagus cuniculus 40-44 14978207-14 2004 The study further describes NHE3 presence simultaneously in multiple dynamic BBM pools in which NHE3 distribution and associated proteins are altered as part of carbachol-induced and Src-mediated rapid signal transduction, which decreases the amount of BBM NHE3 and thus inhibits NHE3 activity. Carbachol 161-170 sodium/hydrogen exchanger 3 Oryctolagus cuniculus 28-32 14978207-4 2004 Carbachol decreased the amount of ileal Na(+) absorptive cell BBM NHE3 within 10 min of exposure. Carbachol 0-9 sodium/hydrogen exchanger 3 Oryctolagus cuniculus 66-70 14978207-14 2004 The study further describes NHE3 presence simultaneously in multiple dynamic BBM pools in which NHE3 distribution and associated proteins are altered as part of carbachol-induced and Src-mediated rapid signal transduction, which decreases the amount of BBM NHE3 and thus inhibits NHE3 activity. Carbachol 161-170 sodium/hydrogen exchanger 3 Oryctolagus cuniculus 96-100 14978207-5 2004 Based on OptiPrep gradient centrifugation, carbachol increased the amount of NHE3 in early endosomes and decreased the amount of NHE3 in BBM, consistent with effects on NHE3 trafficking. Carbachol 43-52 sodium/hydrogen exchanger 3 Oryctolagus cuniculus 77-81 14978207-14 2004 The study further describes NHE3 presence simultaneously in multiple dynamic BBM pools in which NHE3 distribution and associated proteins are altered as part of carbachol-induced and Src-mediated rapid signal transduction, which decreases the amount of BBM NHE3 and thus inhibits NHE3 activity. Carbachol 161-170 proto-oncogene tyrosine-protein kinase Src Oryctolagus cuniculus 183-186 14978207-14 2004 The study further describes NHE3 presence simultaneously in multiple dynamic BBM pools in which NHE3 distribution and associated proteins are altered as part of carbachol-induced and Src-mediated rapid signal transduction, which decreases the amount of BBM NHE3 and thus inhibits NHE3 activity. Carbachol 161-170 sodium/hydrogen exchanger 3 Oryctolagus cuniculus 96-100 14978207-5 2004 Based on OptiPrep gradient centrifugation, carbachol increased the amount of NHE3 in early endosomes and decreased the amount of NHE3 in BBM, consistent with effects on NHE3 trafficking. Carbachol 43-52 sodium/hydrogen exchanger 3 Oryctolagus cuniculus 129-133 14978207-14 2004 The study further describes NHE3 presence simultaneously in multiple dynamic BBM pools in which NHE3 distribution and associated proteins are altered as part of carbachol-induced and Src-mediated rapid signal transduction, which decreases the amount of BBM NHE3 and thus inhibits NHE3 activity. Carbachol 161-170 sodium/hydrogen exchanger 3 Oryctolagus cuniculus 96-100 14978207-5 2004 Based on OptiPrep gradient centrifugation, carbachol increased the amount of NHE3 in early endosomes and decreased the amount of NHE3 in BBM, consistent with effects on NHE3 trafficking. Carbachol 43-52 sodium/hydrogen exchanger 3 Oryctolagus cuniculus 129-133 14978207-7 2004 The size of BBM NHE3 complexes increased in carbachol-exposed ileum, as studied with sucrose gradient centrifugation. Carbachol 44-53 sodium/hydrogen exchanger 3 Oryctolagus cuniculus 16-20 14978207-9 2004 This suggests that carbachol treatment enhanced the association of proteins with NHE3 complexes specifically in the detergent-resistant fraction of ileal BBM. Carbachol 19-28 sodium/hydrogen exchanger 3 Oryctolagus cuniculus 81-85 14978207-10 2004 NHERF2, alpha-actinin-4 and protein kinase C were among those NHE3-associated proteins because they were more efficiently coimmunoprecipitated from total BBM after carbachol treatment. Carbachol 164-173 sodium/hydrogen exchanger 3 Oryctolagus cuniculus 62-66 14978207-11 2004 Moreover, Src was involved in the carbachol-mediated inhibition since: (1) c-Src was rapidly activated in the detergent-resistant membranes by carbachol; and (2) carbachol inhibition of ileal Na(+) absorption was completely abolished by the Src family inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2). Carbachol 34-43 proto-oncogene tyrosine-protein kinase Src Oryctolagus cuniculus 10-13 14978207-11 2004 Moreover, Src was involved in the carbachol-mediated inhibition since: (1) c-Src was rapidly activated in the detergent-resistant membranes by carbachol; and (2) carbachol inhibition of ileal Na(+) absorption was completely abolished by the Src family inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2). Carbachol 34-43 proto-oncogene tyrosine-protein kinase Src Oryctolagus cuniculus 77-80 14978207-11 2004 Moreover, Src was involved in the carbachol-mediated inhibition since: (1) c-Src was rapidly activated in the detergent-resistant membranes by carbachol; and (2) carbachol inhibition of ileal Na(+) absorption was completely abolished by the Src family inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2). Carbachol 34-43 proto-oncogene tyrosine-protein kinase Src Oryctolagus cuniculus 77-80 14978207-11 2004 Moreover, Src was involved in the carbachol-mediated inhibition since: (1) c-Src was rapidly activated in the detergent-resistant membranes by carbachol; and (2) carbachol inhibition of ileal Na(+) absorption was completely abolished by the Src family inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2). Carbachol 143-152 proto-oncogene tyrosine-protein kinase Src Oryctolagus cuniculus 10-13 14978207-11 2004 Moreover, Src was involved in the carbachol-mediated inhibition since: (1) c-Src was rapidly activated in the detergent-resistant membranes by carbachol; and (2) carbachol inhibition of ileal Na(+) absorption was completely abolished by the Src family inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2). Carbachol 143-152 proto-oncogene tyrosine-protein kinase Src Oryctolagus cuniculus 77-80 15020229-5 2004 Glutamate was a potent activator of PLD in neurons but not in astrocytes, whereas noradrenaline and carbachol increased PLD activity only in astrocytes. Carbachol 100-109 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 120-123 15023564-12 2004 They also displayed a carbachol-induced contractility in a medium containing IGF1. Carbachol 22-31 insulin like growth factor 1 Homo sapiens 77-81 15048931-10 2004 Carbachol also increased BDNF mRNA levels without changing NGF or NT(3). Carbachol 0-9 brain derived neurotrophic factor Homo sapiens 25-29 14752030-8 2004 ISO-activated systolic function was inhibited 85% by concomitant muscarinic stimulation (carbachol) in NOS3(-/-)+AdV(NOS3) but not NOS3(-/-)+AdVbeta(gal) hearts. Carbachol 89-98 nitric oxide synthase 3, endothelial cell Mus musculus 103-107 14736881-3 2004 Ca(2+) signals generated by TRPC3 overexpression in HEK293 cells were found to be dependent on extracellular Na(+), in that carbachol-stimulated Ca(2+) entry into TRPC3 expressing cells was significantly suppressed when extracellular Na(+) was reduced to 5 mm. Carbachol 124-133 transient receptor potential cation channel subfamily C member 3 Homo sapiens 28-33 14736881-3 2004 Ca(2+) signals generated by TRPC3 overexpression in HEK293 cells were found to be dependent on extracellular Na(+), in that carbachol-stimulated Ca(2+) entry into TRPC3 expressing cells was significantly suppressed when extracellular Na(+) was reduced to 5 mm. Carbachol 124-133 transient receptor potential cation channel subfamily C member 3 Homo sapiens 163-168 14736881-6 2004 Similar rises in Ca(2+)(i) were recorded in TRPC3-overexpressing cells upon the reduction of extracellular Na(+) subsequent to stimulation with carbachol. Carbachol 144-153 transient receptor potential cation channel subfamily C member 3 Homo sapiens 44-49 14988028-1 2004 An earlier study showed that the neuropeptide Y (NPY) receptor antagonist PYX-2 blocks the enhancement of a carbachol (CCh)-evoked pressor response produced by prior NPY administration into the posterior hypothalamic nucleus (PHN). Carbachol 108-117 neuropeptide Y Rattus norvegicus 49-52 14988028-1 2004 An earlier study showed that the neuropeptide Y (NPY) receptor antagonist PYX-2 blocks the enhancement of a carbachol (CCh)-evoked pressor response produced by prior NPY administration into the posterior hypothalamic nucleus (PHN). Carbachol 108-117 neuropeptide Y Rattus norvegicus 166-169 14988028-1 2004 An earlier study showed that the neuropeptide Y (NPY) receptor antagonist PYX-2 blocks the enhancement of a carbachol (CCh)-evoked pressor response produced by prior NPY administration into the posterior hypothalamic nucleus (PHN). Carbachol 119-122 neuropeptide Y Rattus norvegicus 49-52 14988028-1 2004 An earlier study showed that the neuropeptide Y (NPY) receptor antagonist PYX-2 blocks the enhancement of a carbachol (CCh)-evoked pressor response produced by prior NPY administration into the posterior hypothalamic nucleus (PHN). Carbachol 119-122 neuropeptide Y Rattus norvegicus 166-169 14724209-4 2004 An analysis of mutant channels expressed in Xenopus oocytes revealed two amino acid substitutions in the C-terminal domain of GIRK2, GIRK2(L344E) and GIRK2(G347H), that exhibited decreased carbachol-activated currents but significantly enhanced basal currents with coexpression of G(betagamma) subunits. Carbachol 189-198 potassium inwardly rectifying channel subfamily J member 6 Homo sapiens 126-131 14724209-4 2004 An analysis of mutant channels expressed in Xenopus oocytes revealed two amino acid substitutions in the C-terminal domain of GIRK2, GIRK2(L344E) and GIRK2(G347H), that exhibited decreased carbachol-activated currents but significantly enhanced basal currents with coexpression of G(betagamma) subunits. Carbachol 189-198 potassium inwardly rectifying channel subfamily J member 6 Homo sapiens 133-138 14724209-4 2004 An analysis of mutant channels expressed in Xenopus oocytes revealed two amino acid substitutions in the C-terminal domain of GIRK2, GIRK2(L344E) and GIRK2(G347H), that exhibited decreased carbachol-activated currents but significantly enhanced basal currents with coexpression of G(betagamma) subunits. Carbachol 189-198 potassium inwardly rectifying channel subfamily J member 6 Homo sapiens 133-138 14724209-5 2004 Combining the two mutations (GIRK2(EH)) led to a more severe reduction in carbachol-activated and G(betagamma)-stimulated currents. Carbachol 74-83 potassium inwardly rectifying channel subfamily J member 6 Homo sapiens 29-34 14724209-7 2004 Both GIRK2(L344E) and GIRK2(EH) also showed reduced carbachol activation and normal ethanol activation when expressed in HEK-293T cells. Carbachol 52-61 potassium inwardly rectifying channel subfamily J member 6 Homo sapiens 5-10 14724209-7 2004 Both GIRK2(L344E) and GIRK2(EH) also showed reduced carbachol activation and normal ethanol activation when expressed in HEK-293T cells. Carbachol 52-61 potassium inwardly rectifying channel subfamily J member 6 Homo sapiens 22-27 15196660-3 2004 Time course studies revealed peak translocation after 40 min incubation with carbachol for PKCgamma (110% increase from basal, i.e. no carbachol level, P < 0.01), 30 min for phosphorylated PKCbetaII (130%, P < 0.05) and 5 min for non-phosphorylated PKCbetaII (64%, P < 0.05) with no peak for alpha. Carbachol 77-86 protein kinase C, gamma Mus musculus 91-99 15023776-6 2004 Carbachol caused an elevation of the relative amount of E-cadherin in keratinocytes (P<.05) without changing that of plakoglobin (P>.05). Carbachol 0-9 cadherin 1 Homo sapiens 56-66 15023776-7 2004 The phosphorylation level of E-cadherin and plakoglobin was increased by PV IgG, whereas this effect of PV IgG was attenuated in the presence of 0.5mM carbachol. Carbachol 151-160 cadherin 1 Homo sapiens 29-39 15152572-0 2004 [Carbachol effect on kinetics of the alpha1-adrenergic contractile response of the rat vas deferens]. Carbachol 1-10 arginine vasopressin Rattus norvegicus 87-90 14662757-7 2004 The carbachol concentration at which TRPC6 externalization occurred was lower than the concentration required to activate TRPC6. Carbachol 4-13 transient receptor potential cation channel subfamily C member 6 Homo sapiens 37-42 14662757-7 2004 The carbachol concentration at which TRPC6 externalization occurred was lower than the concentration required to activate TRPC6. Carbachol 4-13 transient receptor potential cation channel subfamily C member 6 Homo sapiens 122-127 14706287-2 2004 We have shown that RNAi knock down of either the inositol 1,4,5-trisphosphate receptor (Ins(1,4,5)P(3)R), or the SERCA calcium pump in the S2-DM1 cells blocks the increase in intracellular calcium concentration ([Ca(2+)](i)) resulting from activation of the DM1 receptor by 100 microM carbamylcholine (CCh). Carbachol 285-300 muscarinic Acetylcholine Receptor, A-type Drosophila melanogaster 142-145 14706287-2 2004 We have shown that RNAi knock down of either the inositol 1,4,5-trisphosphate receptor (Ins(1,4,5)P(3)R), or the SERCA calcium pump in the S2-DM1 cells blocks the increase in intracellular calcium concentration ([Ca(2+)](i)) resulting from activation of the DM1 receptor by 100 microM carbamylcholine (CCh). Carbachol 302-305 muscarinic Acetylcholine Receptor, A-type Drosophila melanogaster 142-145 14729360-3 2004 The aim of this study was to determine the agonist activity of PGF2alpha, latanoprost and carbachol (CCh) on the MLCK pathway in isolated bovine iris sphincter and furthermore to investigate the existence of the FP receptor in this tissue. Carbachol 90-99 myosin light chain kinase, smooth muscle Bos taurus 113-117 14729360-3 2004 The aim of this study was to determine the agonist activity of PGF2alpha, latanoprost and carbachol (CCh) on the MLCK pathway in isolated bovine iris sphincter and furthermore to investigate the existence of the FP receptor in this tissue. Carbachol 101-104 myosin light chain kinase, smooth muscle Bos taurus 113-117 14729360-6 2004 The data obtained on the MLCK pathway showed that the three agonists stimulated the biochemical and pharmacological responses in a concentration and time-dependent manner and that the order of potency and efficacy is PGF2alpha>latanoprost>CCh. Carbachol 245-248 myosin light chain kinase, smooth muscle Bos taurus 25-29 14713860-9 2004 The loss of Cav-1 generated caveolae led to significant urogenital changes in male mice (most marked by 12 months of age), namely 1) bladder weight-to-body weight ratios were increased, 2) the bladder smooth muscle layer was thickened, 3) the bladders had increased baseline, threshold and spontaneous pressures, 4) bladder strips showed a decreased contractile response to carbachol and KCl, and 5) these smooth muscle changes were accompanied by marked fluid accumulation in the prostate and seminal vesicles, with intracellular vacuolization in the kidneys. Carbachol 374-383 caveolin 1, caveolae protein Mus musculus 12-17 14764222-1 2004 OBJECTIVE: To investigate the effects of carbachol injection in intestine on plasma levels of tumor necrosis factor-alpha(TNF-alpha), interleukin-10 (IL-10) and cortisol in rats during gut ischemia/reperfusion. Carbachol 41-50 tumor necrosis factor Rattus norvegicus 122-131 14764222-1 2004 OBJECTIVE: To investigate the effects of carbachol injection in intestine on plasma levels of tumor necrosis factor-alpha(TNF-alpha), interleukin-10 (IL-10) and cortisol in rats during gut ischemia/reperfusion. Carbachol 41-50 interleukin 10 Rattus norvegicus 134-148 14764222-1 2004 OBJECTIVE: To investigate the effects of carbachol injection in intestine on plasma levels of tumor necrosis factor-alpha(TNF-alpha), interleukin-10 (IL-10) and cortisol in rats during gut ischemia/reperfusion. Carbachol 41-50 interleukin 10 Rattus norvegicus 150-155 14764222-5 2004 RESULTS: The plasma levels of TNF-alpha significantly decreased in pretreated and treated groups than those in controls after carbachol injection (both P<0.01). Carbachol 126-135 tumor necrosis factor Rattus norvegicus 30-39 14752030-8 2004 ISO-activated systolic function was inhibited 85% by concomitant muscarinic stimulation (carbachol) in NOS3(-/-)+AdV(NOS3) but not NOS3(-/-)+AdVbeta(gal) hearts. Carbachol 89-98 nitric oxide synthase 3, endothelial cell Mus musculus 117-121 14752030-8 2004 ISO-activated systolic function was inhibited 85% by concomitant muscarinic stimulation (carbachol) in NOS3(-/-)+AdV(NOS3) but not NOS3(-/-)+AdVbeta(gal) hearts. Carbachol 89-98 nitric oxide synthase 3, endothelial cell Mus musculus 117-121 14670369-2 2004 To assess the PLC activity underlying carbachol-induced [Ca(2+)](i) oscillations in single HEK293 cells, we co-imaged [Ca(2+)](i) with fluorescent fusion proteins of protein kinase C (PKC) isotypes and the PH domain of PLC-delta 1 (PLC-delta 1(PH)). Carbachol 38-47 protein kinase C alpha Homo sapiens 184-187 14625299-7 2004 Both PS1 M146V and PS1 L250S cells showed a significant increase in carbachol-induced [Ca2+]i as compared with nontransfected or wild type PS1 transfected cells. Carbachol 68-77 presenilin 1 Homo sapiens 5-8 14625299-7 2004 Both PS1 M146V and PS1 L250S cells showed a significant increase in carbachol-induced [Ca2+]i as compared with nontransfected or wild type PS1 transfected cells. Carbachol 68-77 presenilin 1 Homo sapiens 19-22 14625299-7 2004 Both PS1 M146V and PS1 L250S cells showed a significant increase in carbachol-induced [Ca2+]i as compared with nontransfected or wild type PS1 transfected cells. Carbachol 68-77 presenilin 1 Homo sapiens 19-22 14625299-9 2004 The cells expressing either PS1 D257A or PS1 D385N had attenuated carbachol-stimulated PI hydrolysis and [Ca2+]i responses. Carbachol 66-75 presenilin 1 Homo sapiens 28-31 14625299-9 2004 The cells expressing either PS1 D257A or PS1 D385N had attenuated carbachol-stimulated PI hydrolysis and [Ca2+]i responses. Carbachol 66-75 presenilin 1 Homo sapiens 41-44 14670369-2 2004 To assess the PLC activity underlying carbachol-induced [Ca(2+)](i) oscillations in single HEK293 cells, we co-imaged [Ca(2+)](i) with fluorescent fusion proteins of protein kinase C (PKC) isotypes and the PH domain of PLC-delta 1 (PLC-delta 1(PH)). Carbachol 38-47 phospholipase C delta 1 Homo sapiens 219-230 14670369-2 2004 To assess the PLC activity underlying carbachol-induced [Ca(2+)](i) oscillations in single HEK293 cells, we co-imaged [Ca(2+)](i) with fluorescent fusion proteins of protein kinase C (PKC) isotypes and the PH domain of PLC-delta 1 (PLC-delta 1(PH)). Carbachol 38-47 phospholipase C delta 1 Homo sapiens 232-243 15315164-5 2004 The results showed that as compared with control group, M3 cholinergic receptor agonist (10(-3) mol/L, 10(-4) mol/L carbachol) could induce a concentration-dependent and time-dependent increase in the expression of MOB-1, MCP-1 mRNA in pancreatic acinar cells. Carbachol 116-125 multigenic obesity QTL 1 Mus musculus 215-220 15315164-5 2004 The results showed that as compared with control group, M3 cholinergic receptor agonist (10(-3) mol/L, 10(-4) mol/L carbachol) could induce a concentration-dependent and time-dependent increase in the expression of MOB-1, MCP-1 mRNA in pancreatic acinar cells. Carbachol 116-125 mast cell protease 1 Mus musculus 222-227 15315164-6 2004 After treatment with 10(-3) mol/L carbachol for 2 h, the expression of MOB-1, MCP-1 mRNA was strongest. Carbachol 34-43 multigenic obesity QTL 1 Mus musculus 71-76 15315164-6 2004 After treatment with 10(-3) mol/L carbachol for 2 h, the expression of MOB-1, MCP-1 mRNA was strongest. Carbachol 34-43 mast cell protease 1 Mus musculus 78-83 15315164-7 2004 The activity of NF-kappaB in pancreatic acinar cells was significantly increased (P<0.01) after treated with M3 cholinergic receptor agonist (10(-3) mol/L carbachol) in vitro for 30 min. Carbachol 158-167 nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 Mus musculus 16-25 15315164-8 2004 Either M3 cholinergic receptor antagonist (10(-5) mol/L atropine) or NF-kappaB inhibitor (10(-2) mol/L PDTC) could obviously inhibit the activation of NF-kappaB and the chemokine MOB-1, MCP-1 mRNA expression induced by carbachol (P<0.05). Carbachol 219-228 nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 Mus musculus 69-78 15315164-8 2004 Either M3 cholinergic receptor antagonist (10(-5) mol/L atropine) or NF-kappaB inhibitor (10(-2) mol/L PDTC) could obviously inhibit the activation of NF-kappaB and the chemokine MOB-1, MCP-1 mRNA expression induced by carbachol (P<0.05). Carbachol 219-228 nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 Mus musculus 151-160 15477126-4 2004 Our results indicate that the cholinergic agonist acetylcholine (ACh) and its analog carbachol (CCh) exhibited comparable CCRC profiles in contracting isolated tracheae from both WT and ITK-/- mice, with no alteration in their efficacies. Carbachol 85-94 IL2 inducible T cell kinase Mus musculus 186-189 15390173-4 2004 Carbachol (CCh, applied to the bath) induced a decrease in the field responses (40-50% at 50 microM; 60% at 100 microM) to CA1, presubicular (PreS), and medial entorhinal (MEC) stimulation. Carbachol 0-9 carbonic anhydrase 1 Rattus norvegicus 123-126 15390173-4 2004 Carbachol (CCh, applied to the bath) induced a decrease in the field responses (40-50% at 50 microM; 60% at 100 microM) to CA1, presubicular (PreS), and medial entorhinal (MEC) stimulation. Carbachol 11-14 carbonic anhydrase 1 Rattus norvegicus 123-126 15390173-8 2004 When CA1 afferents were repetitively activated with submaximal stimuli in the presence of CCh, population excitatory postsynaptic potentials (EPSPs) showed modest summation, but every response was smaller than a corresponding events in normal media. Carbachol 90-93 carbonic anhydrase 1 Rattus norvegicus 5-8 15390173-11 2004 We conclude that CA1, PreS, and MEC afferents to the subiculum exhibit CCh sensitivity similar to that established for area CA3 afferents to CA1, and LEC afferents to subiculum exhibit CCh resistance. Carbachol 71-74 carbonic anhydrase 1 Rattus norvegicus 17-20 14512413-3 2003 When ileal smooth muscle strips were cultured with IL-1beta (10 ng/ml), contractions elicited by high K+ and carbachol were inhibited in a time-dependent manner. Carbachol 109-118 interleukin 1 beta Rattus norvegicus 51-59 14597600-3 2003 In murine tracheal rings, IL-13 (100 ng ml-1, 24 h) significantly increased both the carbachol- and KCl-induced maximal force generation without affecting ASM sensitivity. Carbachol 85-94 interleukin 13 Mus musculus 26-31 14597600-4 2003 In cultured human ASM cells, IL-13 (50 ng ml-1, 24 h) also augmented cytosolic calcium levels to bradykinin, histamine and carbachol by 60, 35 and 26%, respectively. Carbachol 123-132 interleukin 13 Homo sapiens 29-34 14575865-2 2003 In this study, we show that PAK1 can be stimulated by carbachol, lysophosphatidic acid (LPA), epidermal growth factor (EGF), and phorbol 12-myristate 13-acetate (PMA) by multiple independent and overlapping pathways. Carbachol 54-63 p21 (RAC1) activated kinase 1 Homo sapiens 28-32 14717603-9 2004 For the dominant, rapidly desensitizing isoform, the carbamoylcholine dissociation constant for the site controlling receptor activation, Kd, is 2 mM; the channel-opening equilibrium constant, Phi(-1), is 4; and the dominant desensitization rate constant, k34, is 20 s(-1). Carbachol 53-69 protein phosphatase 1 regulatory inhibitor subunit 14B Homo sapiens 193-199 14717603-9 2004 For the dominant, rapidly desensitizing isoform, the carbamoylcholine dissociation constant for the site controlling receptor activation, Kd, is 2 mM; the channel-opening equilibrium constant, Phi(-1), is 4; and the dominant desensitization rate constant, k34, is 20 s(-1). Carbachol 53-69 keratin 34 Homo sapiens 256-259 14512413-4 2003 IL-1beta more strongly inhibited the carbachol-induced contractions than high K+ with decreasing myosin light chain phosphorylation. Carbachol 37-46 interleukin 1 beta Rattus norvegicus 0-8 14512413-5 2003 In the alpha-toxin-permeabilized ileal muscle, carbachol with GTP or guanosine 5"-3-O-(thio)triphosphate increased the Ca2+ sensitivity of contractile elements, and this G protein-coupled Ca2+ sensitization was significantly reduced in the IL-1beta-treated ileum. Carbachol 47-56 interleukin 1 beta Rattus norvegicus 240-248 14512413-7 2003 The phosphorylation level of CPI-17 by carbachol was low in accordance with the decrease in CPI-17 expression due to IL-1beta treatment. Carbachol 39-48 protein phosphatase 1, regulatory (inhibitor) subunit 14A Rattus norvegicus 29-35 12880387-3 2003 In the present study, fast-scanning confocal microscopy revealed that activation of mu-opioid receptors alone by 1 muM DAMGO ([L-Ala, NMe-Phe, Gly-ol]-enkephalin) did not stimulate the Ins P3-dependent elementary Ca2+-signalling events (Ca2+ puffs), whereas DAMGO did evoke Ca2+ puffs when applied during concomitant activation of M3 muscarinic receptors with 1 muM carbachol. Carbachol 366-375 latexin Homo sapiens 115-118 12827518-3 2003 Activation of S2-DM1 receptors using CCh resulted in an increase in intracellular calcium ([Ca(2+)](i)) that was biphasic. Carbachol 37-40 muscarinic Acetylcholine Receptor, A-type Drosophila melanogaster 17-20 12827518-5 2003 Spatiotemporal imaging of individual S2-DM1 cells showed that the CCh-induced [Ca(2+)](i) transient resulted from a homogeneous calcium increase throughout the cell, indicative of calcium release from internal stores. Carbachol 66-69 muscarinic Acetylcholine Receptor, A-type Drosophila melanogaster 40-43 14629627-4 2003 Exposure to CCh diminished the cells" ability to elevate cytosolic Ca2+ and secrete beta-hexosaminidase in response to acute stimulation with 100 microM CCh, but it enhanced their secretory responses to phenylephrine and ionomycin. Carbachol 12-15 O-GlcNAcase Homo sapiens 84-103 14534236-3 2003 We mapped the distribution of receptors on the membrane of rat hippocampal CA1 stratum radiatum interneurons and pyramidal cells in acute slices by recording nAChR-mediated currents elicited by local UV laser-based photolysis of caged carbachol in patch-clamped neurons. Carbachol 235-244 carbonic anhydrase 1 Rattus norvegicus 75-78 14534236-3 2003 We mapped the distribution of receptors on the membrane of rat hippocampal CA1 stratum radiatum interneurons and pyramidal cells in acute slices by recording nAChR-mediated currents elicited by local UV laser-based photolysis of caged carbachol in patch-clamped neurons. Carbachol 235-244 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 158-163 14517795-3 2003 We examined whether GH inhibits chloride secretion induced by carbachol (CCh, a calcium-dependent pathway), and the downstream effectors responsible. Carbachol 62-71 growth hormone 1 Homo sapiens 20-22 14517795-3 2003 We examined whether GH inhibits chloride secretion induced by carbachol (CCh, a calcium-dependent pathway), and the downstream effectors responsible. Carbachol 73-76 growth hormone 1 Homo sapiens 20-22 14517795-7 2003 RESULTS: GH inhibited CCh-induced chloride secretion at up to 10 nmol/L, but higher concentrations were less effective. Carbachol 22-25 growth hormone 1 Homo sapiens 9-11 14517795-14 2003 CONCLUSIONS: GH inhibits CCh-induced chloride secretion via a JAK2-dependent mechanism involving transactivation of EGFr and consequent recruitment of ERK1/2. Carbachol 25-28 growth hormone 1 Homo sapiens 13-15 14517795-14 2003 CONCLUSIONS: GH inhibits CCh-induced chloride secretion via a JAK2-dependent mechanism involving transactivation of EGFr and consequent recruitment of ERK1/2. Carbachol 25-28 Janus kinase 2 Homo sapiens 62-66 14517795-14 2003 CONCLUSIONS: GH inhibits CCh-induced chloride secretion via a JAK2-dependent mechanism involving transactivation of EGFr and consequent recruitment of ERK1/2. Carbachol 25-28 epidermal growth factor receptor Homo sapiens 116-120 14517795-14 2003 CONCLUSIONS: GH inhibits CCh-induced chloride secretion via a JAK2-dependent mechanism involving transactivation of EGFr and consequent recruitment of ERK1/2. Carbachol 25-28 mitogen-activated protein kinase 3 Homo sapiens 151-157 14519434-9 2003 The carbachol (10 micromol/l)-induced increase in aaGTP binding was significantly higher in RA than in LV for Goalpha-1/3 (336 +/- 95% of LV, n = 4) and for Gialpha-3 (211 +/- 83%), lower for Gialpha-2 (42 +/- 5%), and was similar in both regions for Goalpha-2 (130 +/- 62%). Carbachol 4-13 tripartite motif containing 47 Homo sapiens 110-117 14519434-9 2003 The carbachol (10 micromol/l)-induced increase in aaGTP binding was significantly higher in RA than in LV for Goalpha-1/3 (336 +/- 95% of LV, n = 4) and for Gialpha-3 (211 +/- 83%), lower for Gialpha-2 (42 +/- 5%), and was similar in both regions for Goalpha-2 (130 +/- 62%). Carbachol 4-13 G protein subunit alpha i3 Homo sapiens 157-166 14519434-9 2003 The carbachol (10 micromol/l)-induced increase in aaGTP binding was significantly higher in RA than in LV for Goalpha-1/3 (336 +/- 95% of LV, n = 4) and for Gialpha-3 (211 +/- 83%), lower for Gialpha-2 (42 +/- 5%), and was similar in both regions for Goalpha-2 (130 +/- 62%). Carbachol 4-13 tripartite motif containing 47 Homo sapiens 251-258 12893840-4 2003 In cultured hippocampal slices, CX614, a second ampakine CX546, and the cholinergic agonist carbachol each increased BDNF mRNA levels with acute (3-h) treatment. Carbachol 92-101 brain-derived neurotrophic factor Rattus norvegicus 117-121 14629627-4 2003 Exposure to CCh diminished the cells" ability to elevate cytosolic Ca2+ and secrete beta-hexosaminidase in response to acute stimulation with 100 microM CCh, but it enhanced their secretory responses to phenylephrine and ionomycin. Carbachol 153-156 O-GlcNAcase Homo sapiens 84-103 14500755-5 2003 The inhibition of Kir2.1 by carbachol was reversible and atropine-sensitive. Carbachol 28-37 potassium inwardly rectifying channel subfamily J member 2 Homo sapiens 18-24 14500755-6 2003 Cotransfection with a dominant-negative mutant of the small GTPase Rho abolished the inhibition of Kir2.1 with current amplitudes remaining at control levels in the presence of carbachol. Carbachol 177-186 potassium inwardly rectifying channel subfamily J member 2 Homo sapiens 99-105 14500755-8 2003 To further confirm the involvement of Rho in the signal transduction pathway, cotransfection with C3 transferase (EFC3), a selective inhibitor of Rho, abolished the reduction in Kir2.1 currents noted upon application of carbachol under control conditions. Carbachol 220-229 potassium inwardly rectifying channel subfamily J member 2 Homo sapiens 178-184 13679249-8 2003 Repeated injections of carbachol (30 pmol) into the LSD produced Fos immunoreactivity in the ipsilateral side of the LSV. Carbachol 23-32 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 65-68 12807915-3 2003 Purified G beta gamma, alone or with phosphatidylinositol 4,5-bisphosphate (PIP2), inhibited carbachol-evoked membrane recruitment of tubulin and G alpha q transactivation by tubulin. Carbachol 93-102 succinate-CoA ligase GDP-forming subunit beta Homo sapiens 9-15 12807915-3 2003 Purified G beta gamma, alone or with phosphatidylinositol 4,5-bisphosphate (PIP2), inhibited carbachol-evoked membrane recruitment of tubulin and G alpha q transactivation by tubulin. Carbachol 93-102 G protein subunit alpha q Homo sapiens 146-155 12807915-4 2003 Polymerization of microtubules elicited by G beta gamma overrode tubulin translocation to the membrane in response to carbachol stimulation. Carbachol 118-127 succinate-CoA ligase GDP-forming subunit beta Homo sapiens 43-49 12807915-8 2003 Both confocal microscopy and coimmunoprecipitation studies revealed the spatiotemporal pattern of G beta gamma/tubulin interaction during carbachol stimulation of neuroblastoma SK-N-SH cells. Carbachol 138-147 succinate-CoA ligase GDP-forming subunit beta Homo sapiens 98-104 12807915-11 2003 Fifteen min post-carbachol addition, tubulin and G beta colocalized in vesicle-like structures in the cytosol. Carbachol 17-26 succinate-CoA ligase GDP-forming subunit beta Homo sapiens 49-55 12747804-1 2003 In 1321N1 astrocytoma cells, carbachol stimulation of M3 muscarinic cholinergic receptors, coupled to phospholipase C, evoked a persistent 10-20-fold activation of p70 S6 kinase (S6K1). Carbachol 29-38 ribosomal protein S6 kinase B1 Homo sapiens 179-183 12893847-11 2003 Although forskolin interacted with carbachol, allowing acid secretion in HDC-/- mice, similar results were not achieved with gastrin. Carbachol 35-44 histidine decarboxylase Mus musculus 73-76 12893847-12 2003 Such results suggest that 1) histamine is essential for carbachol- and gastrin-stimulated gastric acid secretion in mice; and 2) histamine-induced cAMP production contributes to the in vivo response to carbachol or gastrin. Carbachol 202-211 gastrin Mus musculus 71-78 12890474-5 2003 Activation of cholinergic receptors in cultured chick atrial myocytes by carbachol produced an outward potassium current (I(K(ACh))), which was attenuated by 24-48-h pre-treatment with neuregulin-1. Carbachol 73-82 neuregulin 1 Gallus gallus 185-197 12922936-6 2003 Induction of inducible nitric oxide synthase (iNOS) impaired the stimulated NO synthesis from eNOS (100 nM carbachol-stimulated NO: control 5.7+/-0.6, iNOS 0.3+/-0.3 nM). Carbachol 107-116 nitric oxide synthase 2 Rattus norvegicus 13-44 12922936-6 2003 Induction of inducible nitric oxide synthase (iNOS) impaired the stimulated NO synthesis from eNOS (100 nM carbachol-stimulated NO: control 5.7+/-0.6, iNOS 0.3+/-0.3 nM). Carbachol 107-116 nitric oxide synthase 2 Rattus norvegicus 46-50 12922936-6 2003 Induction of inducible nitric oxide synthase (iNOS) impaired the stimulated NO synthesis from eNOS (100 nM carbachol-stimulated NO: control 5.7+/-0.6, iNOS 0.3+/-0.3 nM). Carbachol 107-116 nitric oxide synthase 2 Rattus norvegicus 151-155 12922936-9 2003 Impairment of eNOS by iNOS was also prevented by L-arginine 100 micro M administered simultaneously with carbachol, but not by L-arginine administered during incubation with lipopolysaccharide. Carbachol 105-114 nitric oxide synthase 2 Rattus norvegicus 22-26 14616247-9 2003 Addition of carbachol (10-4 m) increased average DAF-2 fluorescence by 22.8% and endothelial calcium concentration by 28.9%, whereas the arteriolar diameter remained essentially unchanged. Carbachol 12-21 CD55 molecule (Cromer blood group) Homo sapiens 49-52 12747804-2 2003 This response was abolished by chelation of cytosolic Ca2+ and reproduced by the Ca2+ ionophore ionomycin, but was not prevented by down-regulation or inhibition of protein kinase C. Carbachol-stimulated activation and phosphorylation of S6K1 at Thr389 were prevented by rapamycin, an inhibitor of mTOR (mammalian target of rapamycin), or by wortmannin, a phosphoinositide 3-kinase (PI3K) inhibitor. Carbachol 183-192 ribosomal protein S6 kinase B1 Homo sapiens 238-242 12730147-4 2003 We found that in tracheal rings treated with 50 ng/ml TNF-alpha, carbachol-induced isometric force was significantly increased by 30% compared with those treated with diluent alone (P < 0.05). Carbachol 65-74 tumor necrosis factor Mus musculus 54-63 12747804-2 2003 This response was abolished by chelation of cytosolic Ca2+ and reproduced by the Ca2+ ionophore ionomycin, but was not prevented by down-regulation or inhibition of protein kinase C. Carbachol-stimulated activation and phosphorylation of S6K1 at Thr389 were prevented by rapamycin, an inhibitor of mTOR (mammalian target of rapamycin), or by wortmannin, a phosphoinositide 3-kinase (PI3K) inhibitor. Carbachol 183-192 mechanistic target of rapamycin kinase Homo sapiens 298-302 12747804-2 2003 This response was abolished by chelation of cytosolic Ca2+ and reproduced by the Ca2+ ionophore ionomycin, but was not prevented by down-regulation or inhibition of protein kinase C. Carbachol-stimulated activation and phosphorylation of S6K1 at Thr389 were prevented by rapamycin, an inhibitor of mTOR (mammalian target of rapamycin), or by wortmannin, a phosphoinositide 3-kinase (PI3K) inhibitor. Carbachol 183-192 mechanistic target of rapamycin kinase Homo sapiens 304-333 12747804-2 2003 This response was abolished by chelation of cytosolic Ca2+ and reproduced by the Ca2+ ionophore ionomycin, but was not prevented by down-regulation or inhibition of protein kinase C. Carbachol-stimulated activation and phosphorylation of S6K1 at Thr389 were prevented by rapamycin, an inhibitor of mTOR (mammalian target of rapamycin), or by wortmannin, a phosphoinositide 3-kinase (PI3K) inhibitor. Carbachol 183-192 phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta Homo sapiens 356-381 12747804-3 2003 Carbachol also stimulated the phosphorylation of eukaryotic initiation factor 4E-binding protein-1 (4E-BP1), a second mTOR-dependent event, with similar potency to its effect on S6K1. Carbachol 0-9 eukaryotic translation initiation factor 4E binding protein 1 Homo sapiens 100-106 12747804-3 2003 Carbachol also stimulated the phosphorylation of eukaryotic initiation factor 4E-binding protein-1 (4E-BP1), a second mTOR-dependent event, with similar potency to its effect on S6K1. Carbachol 0-9 mechanistic target of rapamycin kinase Homo sapiens 118-122 12747804-3 2003 Carbachol also stimulated the phosphorylation of eukaryotic initiation factor 4E-binding protein-1 (4E-BP1), a second mTOR-dependent event, with similar potency to its effect on S6K1. Carbachol 0-9 ribosomal protein S6 kinase B1 Homo sapiens 178-182 12747804-6 2003 By contrast, an inhibitor of epidermal growth factor receptor kinase, AG1478, which prevents carbachol-stimulated ErbB3 transactivation, PI3K recruitment and protein kinase B activation in 1321N1 cells, reduced activation of S6K1 by no more than 30%. Carbachol 93-102 erb-b2 receptor tyrosine kinase 3 Homo sapiens 114-119 12747804-6 2003 By contrast, an inhibitor of epidermal growth factor receptor kinase, AG1478, which prevents carbachol-stimulated ErbB3 transactivation, PI3K recruitment and protein kinase B activation in 1321N1 cells, reduced activation of S6K1 by no more than 30%. Carbachol 93-102 ribosomal protein S6 kinase B1 Homo sapiens 225-229 12911748-8 2003 DSI was observed in CA1 pyramidal neurons under control conditions, and its incidence was greatly increased by the cholinergic agonist carbachol. Carbachol 135-144 carbonic anhydrase 1 Rattus norvegicus 20-23 12799421-3 2003 In both cell types, GPI-ACE, but not WT-ACE, was sequestered in caveolin or flotillin-enriched lipid rafts and was released from the cell surface by treatment with phosphatidylinositol-specific phospholipase C. When cells were treated with activators of the protein kinase C signalling cascade (phorbol myristate acetate or carbachol) the shedding of GPI-ACE was stimulated to a similar extent to that of WT-ACE. Carbachol 324-333 angiotensin I converting enzyme Homo sapiens 24-27 12730147-6 2003 The enhancing effect of TNF-alpha on carbachol-induced isometric force generation was completely abrogated in the tracheal rings obtained from TNF-alpha receptor (TNFR)1-deficient mice and in control rings treated with a TNF-alpha mutant that solely activates TNFR2. Carbachol 37-46 tumor necrosis factor Mus musculus 24-33 12730147-6 2003 The enhancing effect of TNF-alpha on carbachol-induced isometric force generation was completely abrogated in the tracheal rings obtained from TNF-alpha receptor (TNFR)1-deficient mice and in control rings treated with a TNF-alpha mutant that solely activates TNFR2. Carbachol 37-46 tumor necrosis factor Mus musculus 143-152 12730147-6 2003 The enhancing effect of TNF-alpha on carbachol-induced isometric force generation was completely abrogated in the tracheal rings obtained from TNF-alpha receptor (TNFR)1-deficient mice and in control rings treated with a TNF-alpha mutant that solely activates TNFR2. Carbachol 37-46 tumor necrosis factor receptor superfamily, member 1a Mus musculus 163-169 12730147-6 2003 The enhancing effect of TNF-alpha on carbachol-induced isometric force generation was completely abrogated in the tracheal rings obtained from TNF-alpha receptor (TNFR)1-deficient mice and in control rings treated with a TNF-alpha mutant that solely activates TNFR2. Carbachol 37-46 tumor necrosis factor Mus musculus 143-152 12730147-6 2003 The enhancing effect of TNF-alpha on carbachol-induced isometric force generation was completely abrogated in the tracheal rings obtained from TNF-alpha receptor (TNFR)1-deficient mice and in control rings treated with a TNF-alpha mutant that solely activates TNFR2. Carbachol 37-46 tumor necrosis factor receptor superfamily, member 1a Mus musculus 260-265 12842132-5 2003 These results suggest that carbachol-activation of M(1) mAChRs increases m1 mAChR, nNOS and iNOS mRNA levels associated with increased production of nitric oxide (NO). Carbachol 27-36 nitric oxide synthase 1 Homo sapiens 83-87 12842132-5 2003 These results suggest that carbachol-activation of M(1) mAChRs increases m1 mAChR, nNOS and iNOS mRNA levels associated with increased production of nitric oxide (NO). Carbachol 27-36 nitric oxide synthase 2 Homo sapiens 92-96 12606302-12 2003 Finally, in parallel with the reduction in SK4 message observed in animals deprived of dietary K+, carbachol-induced 86Rb+ secretion was abolished in dietary K+-depleted animals. Carbachol 99-108 potassium calcium-activated channel subfamily N member 4 Rattus norvegicus 43-46 12732636-13 2003 Cav1Delta51-169 also suppressed thapsigarginand carbachol-stimulated Ca2+ influx and increased the detergent solubility of TRPC1, although plasma membrane lipid raft domains were not disrupted. Carbachol 48-57 caveolin 1 Canis lupus familiaris 0-15 12665513-0 2003 TAO (thousand-and-one amino acid) protein kinases mediate signaling from carbachol to p38 mitogen-activated protein kinase and ternary complex factors. Carbachol 73-82 mitogen-activated protein kinase 14 Homo sapiens 86-122 12665513-2 2003 We found that TAO2 activity was increased by carbachol and that carbachol and the heterotrimeric G protein Galphao could activate p38 in 293 cells. Carbachol 45-54 TAO kinase 2 Homo sapiens 14-18 12665513-2 2003 We found that TAO2 activity was increased by carbachol and that carbachol and the heterotrimeric G protein Galphao could activate p38 in 293 cells. Carbachol 64-73 TAO kinase 2 Homo sapiens 14-18 12665513-2 2003 We found that TAO2 activity was increased by carbachol and that carbachol and the heterotrimeric G protein Galphao could activate p38 in 293 cells. Carbachol 64-73 mitogen-activated protein kinase 14 Homo sapiens 130-133 12665513-3 2003 Using dominant interfering kinase mutants, we found that MEKs 3 and 6 and TAOs were required for p38 activation by carbachol or the constitutively active mutant GalphaoQ205L. Carbachol 115-124 mitogen-activated protein kinase 14 Homo sapiens 97-100 12665513-7 2003 Taken together, these studies suggest that TAO protein kinases relay signals from carbachol through heterotrimeric G proteins to the p38 MAP kinase, which then activates TCFs in the nucleus. Carbachol 82-91 mitogen-activated protein kinase 14 Homo sapiens 133-147 12672827-9 2003 Depletion of intracellular Ca2+ stores by lowering extracellular Ca2+ concentration and treatment with the Ca2+-ATPase inhibitor thapsigargin or the muscarinic receptor agonist carbachol further augmented hTRPM3-mediated Ca2+ entry. Carbachol 177-186 transient receptor potential cation channel subfamily M member 3 Homo sapiens 205-211 12922941-4 2003 The Rho-kinase inhibitor Y27632 produced concentration-dependent decreases in tone raised by either the muscarinic receptor agonist carbachol (CCh), or the sarco-endoplasmic reticulum calcium ATPase inhibitor thapsigargin (Tg) (EC(50) values against CCh and Tg of 8.4+/-3.3 (n=6) and 6.1+/-2.1 (n=7) micro M, respectively). Carbachol 132-141 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 4-14 12922941-4 2003 The Rho-kinase inhibitor Y27632 produced concentration-dependent decreases in tone raised by either the muscarinic receptor agonist carbachol (CCh), or the sarco-endoplasmic reticulum calcium ATPase inhibitor thapsigargin (Tg) (EC(50) values against CCh and Tg of 8.4+/-3.3 (n=6) and 6.1+/-2.1 (n=7) micro M, respectively). Carbachol 143-146 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 4-14 12922941-4 2003 The Rho-kinase inhibitor Y27632 produced concentration-dependent decreases in tone raised by either the muscarinic receptor agonist carbachol (CCh), or the sarco-endoplasmic reticulum calcium ATPase inhibitor thapsigargin (Tg) (EC(50) values against CCh and Tg of 8.4+/-3.3 (n=6) and 6.1+/-2.1 (n=7) micro M, respectively). Carbachol 250-253 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 4-14 12922941-11 2003 Western blot analysis of fractionated tissue samples probed for RhoA immunoreactivity, indicated that both CCh and Tg were able to induce translocation of RhoA from the cytosol to the membrane. Carbachol 107-110 ras homolog family member A Mus musculus 64-68 12922941-11 2003 Western blot analysis of fractionated tissue samples probed for RhoA immunoreactivity, indicated that both CCh and Tg were able to induce translocation of RhoA from the cytosol to the membrane. Carbachol 107-110 ras homolog family member A Mus musculus 155-159 12850283-2 2003 In PRL-treated islets, stimulation by glucose (8 mM), carbamylcholine chloride (CCh) and phorbol dibutyrate increased cAMP levels 40, 89, and 151%, respectively, above similarly stimulated control islets without PRL. Carbachol 54-78 prolactin Rattus norvegicus 3-6 12850283-2 2003 In PRL-treated islets, stimulation by glucose (8 mM), carbamylcholine chloride (CCh) and phorbol dibutyrate increased cAMP levels 40, 89, and 151%, respectively, above similarly stimulated control islets without PRL. Carbachol 54-78 prolactin Rattus norvegicus 212-215 12850283-2 2003 In PRL-treated islets, stimulation by glucose (8 mM), carbamylcholine chloride (CCh) and phorbol dibutyrate increased cAMP levels 40, 89, and 151%, respectively, above similarly stimulated control islets without PRL. Carbachol 80-83 prolactin Rattus norvegicus 3-6 12850283-2 2003 In PRL-treated islets, stimulation by glucose (8 mM), carbamylcholine chloride (CCh) and phorbol dibutyrate increased cAMP levels 40, 89, and 151%, respectively, above similarly stimulated control islets without PRL. Carbachol 80-83 prolactin Rattus norvegicus 212-215 12781924-2 2003 We also observed that, though several signal transduction pathways are relevant for carbachol-induced cell proliferation, activation of PKC zeta and p70S6 kinase is selectively inhibited by low concentrations of ethanol. Carbachol 84-93 protein kinase C zeta Homo sapiens 136-144 12781924-7 2003 Carbachol also induced phosphorylation of (Thr410)PKC zeta, (Ser473)Akt, and (Thr389)p70S6 kinase, and ethanol (50 mM) inhibited phosphorylation of PKC zeta and p70S6 kinase, but not of Akt. Carbachol 0-9 protein kinase C zeta Homo sapiens 50-58 12781924-7 2003 Carbachol also induced phosphorylation of (Thr410)PKC zeta, (Ser473)Akt, and (Thr389)p70S6 kinase, and ethanol (50 mM) inhibited phosphorylation of PKC zeta and p70S6 kinase, but not of Akt. Carbachol 0-9 protein kinase C zeta Homo sapiens 148-156 12781924-7 2003 Carbachol also induced phosphorylation of (Thr410)PKC zeta, (Ser473)Akt, and (Thr389)p70S6 kinase, and ethanol (50 mM) inhibited phosphorylation of PKC zeta and p70S6 kinase, but not of Akt. Carbachol 0-9 AKT serine/threonine kinase 1 Homo sapiens 68-71 12788814-5 2003 (3) Go 6976, an inhibitor of calcium-dependent PKC, concentration-dependently antagonized the inhibitory effect of TPA, and, therefore, revealed the action of PKC-alpha on carbachol-induced acid secretion in rabbit parietal cells. Carbachol 172-181 protein kinase C alpha type Oryctolagus cuniculus 159-168 12745080-1 2003 In isolated rat pancreatic acini, protein expression of RhoA and Rho-associated kinase, ROCK-II, and the formation of immunocomplex of RhoA with ROCK-II were enhanced by CCK-8, carbachol, and the phorbol ester TPA. Carbachol 177-186 ras homolog family member A Rattus norvegicus 56-60 12745080-1 2003 In isolated rat pancreatic acini, protein expression of RhoA and Rho-associated kinase, ROCK-II, and the formation of immunocomplex of RhoA with ROCK-II were enhanced by CCK-8, carbachol, and the phorbol ester TPA. Carbachol 177-186 Rho-associated coiled-coil containing protein kinase 2 Rattus norvegicus 88-95 12745080-1 2003 In isolated rat pancreatic acini, protein expression of RhoA and Rho-associated kinase, ROCK-II, and the formation of immunocomplex of RhoA with ROCK-II were enhanced by CCK-8, carbachol, and the phorbol ester TPA. Carbachol 177-186 ras homolog family member A Rattus norvegicus 135-139 12745080-1 2003 In isolated rat pancreatic acini, protein expression of RhoA and Rho-associated kinase, ROCK-II, and the formation of immunocomplex of RhoA with ROCK-II were enhanced by CCK-8, carbachol, and the phorbol ester TPA. Carbachol 177-186 Rho-associated coiled-coil containing protein kinase 2 Rattus norvegicus 145-152 12652642-0 2003 Human brain synembryn interacts with Gsalpha and Gqalpha and is translocated to the plasma membrane in response to isoproterenol and carbachol. Carbachol 133-142 Synembryn Caenorhabditis elegans 12-21 12743035-3 2003 We demonstrate that in squamous cell carcinoma cells, stimulation with the GPCR agonists LPA or carbachol specifically results in metalloprotease cleavage and release of amphiregulin (AR). Carbachol 96-105 C-X-C motif chemokine receptor 6 Homo sapiens 75-79 12743035-3 2003 We demonstrate that in squamous cell carcinoma cells, stimulation with the GPCR agonists LPA or carbachol specifically results in metalloprotease cleavage and release of amphiregulin (AR). Carbachol 96-105 amphiregulin Homo sapiens 170-182 12743035-3 2003 We demonstrate that in squamous cell carcinoma cells, stimulation with the GPCR agonists LPA or carbachol specifically results in metalloprotease cleavage and release of amphiregulin (AR). Carbachol 96-105 amphiregulin Homo sapiens 184-186 12729842-5 2003 Although carbachol but not gastrin induced in vivo gastric acid production in histamine H(2) receptor-null mice, gastric pH was elevated by both muscarinic M(3) and gastrin antagonists. Carbachol 9-18 histamine receptor H2 Mus musculus 78-101 12772861-4 2003 Carbachol increased (P < 0.10) alpha-amylase and trypsin release in tissue collected from all calves. Carbachol 0-9 alpha amylase Bos taurus 34-47 12748850-6 2003 Carbachol also dose-dependently stimulated extracellular signal-regulated protein kinase (ERK) activation. Carbachol 0-9 mitogen-activated protein kinase 1 Homo sapiens 43-88 12748850-6 2003 Carbachol also dose-dependently stimulated extracellular signal-regulated protein kinase (ERK) activation. Carbachol 0-9 mitogen-activated protein kinase 1 Homo sapiens 90-93 12748850-7 2003 This effect was inhibited by PD98059, an inhibitor of extracellular signal-regulated protein kinase kinase, which also blocked carbachol activation of cell proliferation, indicating that the p21Ras-ERK pathway is an important signaling cascade in the mitogenic effect. Carbachol 127-136 mitogen-activated protein kinase 1 Homo sapiens 54-99 12748850-7 2003 This effect was inhibited by PD98059, an inhibitor of extracellular signal-regulated protein kinase kinase, which also blocked carbachol activation of cell proliferation, indicating that the p21Ras-ERK pathway is an important signaling cascade in the mitogenic effect. Carbachol 127-136 HRas proto-oncogene, GTPase Homo sapiens 191-197 12748850-7 2003 This effect was inhibited by PD98059, an inhibitor of extracellular signal-regulated protein kinase kinase, which also blocked carbachol activation of cell proliferation, indicating that the p21Ras-ERK pathway is an important signaling cascade in the mitogenic effect. Carbachol 127-136 mitogen-activated protein kinase 1 Homo sapiens 198-201 12748850-9 2003 RESULTS: Carbachol induced tyrosine phosphorylation of EGFR, which was abolished by an EGFR tyrosine kinase inhibitor AG1478. Carbachol 9-18 epidermal growth factor receptor Homo sapiens 55-59 12748850-9 2003 RESULTS: Carbachol induced tyrosine phosphorylation of EGFR, which was abolished by an EGFR tyrosine kinase inhibitor AG1478. Carbachol 9-18 epidermal growth factor receptor Homo sapiens 87-91 12748850-10 2003 Transactivation by carbachol was also abrogated by a metalloproteinases (MMPs) inhibitor GM6001 or an EGFR-blocking antibody (LA-1), suggesting that binding of EGFR ligand(s) produced by MMPs may initiate transactivation in a manner dependent on EGFR tyrosine kinase. Carbachol 19-28 epidermal growth factor receptor Homo sapiens 102-106 12748850-10 2003 Transactivation by carbachol was also abrogated by a metalloproteinases (MMPs) inhibitor GM6001 or an EGFR-blocking antibody (LA-1), suggesting that binding of EGFR ligand(s) produced by MMPs may initiate transactivation in a manner dependent on EGFR tyrosine kinase. Carbachol 19-28 epidermal growth factor receptor Homo sapiens 160-164 12748850-10 2003 Transactivation by carbachol was also abrogated by a metalloproteinases (MMPs) inhibitor GM6001 or an EGFR-blocking antibody (LA-1), suggesting that binding of EGFR ligand(s) produced by MMPs may initiate transactivation in a manner dependent on EGFR tyrosine kinase. Carbachol 19-28 epidermal growth factor receptor Homo sapiens 160-164 12692900-4 2003 Stimulation of neural precursor cells dissociated from embryonic day 13 rat cortical neuroepithelium with the muscarinic receptor agonist carbachol (CCh) induced phosphorylations of c-src that were detected by antibodies raised against phospho-Tyr416 (Ptyr416), phospho-Tyr527 (Ptyr527), and phospho-Tyr215 (Ptyr215) of the kinase. Carbachol 138-147 SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus 182-187 12692900-4 2003 Stimulation of neural precursor cells dissociated from embryonic day 13 rat cortical neuroepithelium with the muscarinic receptor agonist carbachol (CCh) induced phosphorylations of c-src that were detected by antibodies raised against phospho-Tyr416 (Ptyr416), phospho-Tyr527 (Ptyr527), and phospho-Tyr215 (Ptyr215) of the kinase. Carbachol 149-152 SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus 182-187 12755612-9 2003 Because the nAChR desensitizes rapidly, to make the required measurements a cell-flow technique with a time resolution of 10 ms was used to equilibrate BCH(3) cells containing the fetal mouse muscle-type nAChR with carbamoylcholine. Carbachol 215-231 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 204-209 12692900-6 2003 Both extracellular signal-regulated kinase (Erk1/2) and CREB were significantly activated after CCh treatment indicated by increases in phosphorylation of these two proteins. Carbachol 96-99 mitogen activated protein kinase 3 Rattus norvegicus 44-50 12692900-6 2003 Both extracellular signal-regulated kinase (Erk1/2) and CREB were significantly activated after CCh treatment indicated by increases in phosphorylation of these two proteins. Carbachol 96-99 cAMP responsive element binding protein 1 Rattus norvegicus 56-60 12692900-7 2003 The c-Src inhibitor PP1 abolished the CCh-induced activation of Erk1/2 and CREB in a dose-dependent manner. Carbachol 38-41 SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus 4-9 12692900-7 2003 The c-Src inhibitor PP1 abolished the CCh-induced activation of Erk1/2 and CREB in a dose-dependent manner. Carbachol 38-41 neuropeptide Y receptor Y4 Rattus norvegicus 20-23 12692900-7 2003 The c-Src inhibitor PP1 abolished the CCh-induced activation of Erk1/2 and CREB in a dose-dependent manner. Carbachol 38-41 mitogen activated protein kinase 3 Rattus norvegicus 64-70 12692900-7 2003 The c-Src inhibitor PP1 abolished the CCh-induced activation of Erk1/2 and CREB in a dose-dependent manner. Carbachol 38-41 cAMP responsive element binding protein 1 Rattus norvegicus 75-79 12692900-8 2003 Moreover, CCh stimulated expression of the neuronal specific marker MAP2, which was inhibited by PP1. Carbachol 10-13 microtubule-associated protein 2 Rattus norvegicus 68-72 12692900-8 2003 Moreover, CCh stimulated expression of the neuronal specific marker MAP2, which was inhibited by PP1. Carbachol 10-13 neuropeptide Y receptor Y4 Rattus norvegicus 97-100 12692900-9 2003 Cell proliferation assays and immunocytochemistry revealed that PP1 inhibited the CCh-induced DNA synthesis and MAP2(+) production. Carbachol 82-85 neuropeptide Y receptor Y4 Rattus norvegicus 64-67 12652642-6 2003 Furthermore, synembryn was shown to translocate to the plasma membrane in response to carbachol and isoproterenol. Carbachol 86-95 Synembryn Caenorhabditis elegans 13-22 12594217-9 2003 An investigation of tyrosine phosphorylation levels of the plasma membrane Ca(2+)-ATPase (PMCA) demonstrated that CCh stimulates an increase in tyrosine phosphorylation levels, which has been reported to inhibit Ca(2+) pump activity, whereas in contrast, BK stimulates a reduction of PMCA tyrosine phosphorylation levels. Carbachol 114-117 ATPase plasma membrane Ca2+ transporting 2 Homo sapiens 59-88 12594217-2 2003 After depletion of Ca(2+) stores by either TG, BK, or CCh, the addition of Ca(2+) gave a much larger rise in Ca(2+) levels in CCh-treated and TG-treated cells than in cells treated with BK. Carbachol 126-129 kininogen 1 Homo sapiens 186-188 12676367-0 2003 Rewarding injections of the cholinergic agonist carbachol into the ventral tegmental area induce locomotion and c-Fos expression in the retrosplenial area and supramammillary nucleus. Carbachol 48-57 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 112-117 12676367-2 2003 To determine what brain regions are activated by such rewarding injections we studied the expression of the transcription factor c-Fos in local and distant brain regions following ventral tegmental injections of carbachol in rats. Carbachol 212-221 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 129-134 12676367-5 2003 Ventral tegmental injections of carbachol induced c-Fos expression throughout the brain. Carbachol 32-41 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 50-55 12670478-2 2003 Two Rho-kinase inhibitors, Y-27632 and fasudil (HA-1077), conspicuously suppressed the contractile responses to carbachol (CCh) and KCl as well as electrical field stimulation (EFS, 40 V, 0.5 ms, and 20 s). Carbachol 112-121 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 4-14 12670478-2 2003 Two Rho-kinase inhibitors, Y-27632 and fasudil (HA-1077), conspicuously suppressed the contractile responses to carbachol (CCh) and KCl as well as electrical field stimulation (EFS, 40 V, 0.5 ms, and 20 s). Carbachol 123-126 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 4-14 12670478-5 2003 The Rho-kinase inhibitors relaxed the fundic strips preconstricted by submaximal concentration of CCh or KCl in a concentration dependent manner. Carbachol 98-101 Rho-associated coiled-coil containing protein kinase 2 Mus musculus 4-14 12529321-11 2003 Surprisingly, carbachol-stimulated degranulation was blocked by antibody-mediated inhibition of the Class III PI 3-kinase hVPS34 or by titration of its product with FYVE domains. Carbachol 14-23 phosphatidylinositol 3-kinase catalytic subunit type 3 Homo sapiens 122-128 12620895-9 2003 Carbachol also increased tyrosine phosphorylation of Pyk2, p60Src, and the epidermal growth factor receptor (EGFR). Carbachol 0-9 protein tyrosine kinase 2 beta Rattus norvegicus 53-57 12620895-9 2003 Carbachol also increased tyrosine phosphorylation of Pyk2, p60Src, and the epidermal growth factor receptor (EGFR). Carbachol 0-9 SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus 59-65 12620895-9 2003 Carbachol also increased tyrosine phosphorylation of Pyk2, p60Src, and the epidermal growth factor receptor (EGFR). Carbachol 0-9 epidermal growth factor receptor Rattus norvegicus 75-107 12620895-9 2003 Carbachol also increased tyrosine phosphorylation of Pyk2, p60Src, and the epidermal growth factor receptor (EGFR). Carbachol 0-9 epidermal growth factor receptor Rattus norvegicus 109-113 12620895-10 2003 The Src inhibitor PP1 and the EGFR inhibitor AG-1478 completely inhibited carbachol-stimulated MAPK activation. Carbachol 74-83 SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus 4-7 12620895-10 2003 The Src inhibitor PP1 and the EGFR inhibitor AG-1478 completely inhibited carbachol-stimulated MAPK activation. Carbachol 74-83 neuropeptide Y receptor Y4 Rattus norvegicus 18-21 12620895-10 2003 The Src inhibitor PP1 and the EGFR inhibitor AG-1478 completely inhibited carbachol-stimulated MAPK activation. Carbachol 74-83 epidermal growth factor receptor Rattus norvegicus 30-34 12620895-12 2003 We conclude that carbachol transactivates the EGFR to activate MAPK, leading to conjunctival goblet cell secretion. Carbachol 17-26 epidermal growth factor receptor Rattus norvegicus 46-50 12620895-13 2003 In addition, carbachol also activates Pyk2 and p60Src that could play a role in the transactivation of the EGFR. Carbachol 13-22 protein tyrosine kinase 2 beta Rattus norvegicus 38-42 12620895-13 2003 In addition, carbachol also activates Pyk2 and p60Src that could play a role in the transactivation of the EGFR. Carbachol 13-22 SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus 47-53 12620895-13 2003 In addition, carbachol also activates Pyk2 and p60Src that could play a role in the transactivation of the EGFR. Carbachol 13-22 epidermal growth factor receptor Rattus norvegicus 107-111 12631560-6 2003 Both NSCC activated by CCh in murine stomach and mTRP5 were inhibited by intracellularly applied anti-G(q/11) antibody, PLC inhibitor U-73122, IICR inhibitor 2-aminoethoxydiphenylborate, and nonspecific cation channel blockers La(3+) and flufenamate. Carbachol 23-26 perlecan (heparan sulfate proteoglycan 2) Mus musculus 120-123 12631560-10 2003 From the above results, we suggest that mTRP5 might be a candidate for the NSCC activated by ACh or CCh in murine stomach. Carbachol 100-103 transient receptor potential cation channel, subfamily C, member 5 Mus musculus 40-45 12721115-9 2003 Moreover, treatment of OA-sensitized mice with HOE-140 (100 microg kg(-1)) completely abolished the AHR to carbachol. Carbachol 107-116 aryl-hydrocarbon receptor Mus musculus 100-103 12594217-9 2003 An investigation of tyrosine phosphorylation levels of the plasma membrane Ca(2+)-ATPase (PMCA) demonstrated that CCh stimulates an increase in tyrosine phosphorylation levels, which has been reported to inhibit Ca(2+) pump activity, whereas in contrast, BK stimulates a reduction of PMCA tyrosine phosphorylation levels. Carbachol 114-117 ATPase plasma membrane Ca2+ transporting 2 Homo sapiens 90-94 12594217-9 2003 An investigation of tyrosine phosphorylation levels of the plasma membrane Ca(2+)-ATPase (PMCA) demonstrated that CCh stimulates an increase in tyrosine phosphorylation levels, which has been reported to inhibit Ca(2+) pump activity, whereas in contrast, BK stimulates a reduction of PMCA tyrosine phosphorylation levels. Carbachol 114-117 kininogen 1 Homo sapiens 255-257 12594217-9 2003 An investigation of tyrosine phosphorylation levels of the plasma membrane Ca(2+)-ATPase (PMCA) demonstrated that CCh stimulates an increase in tyrosine phosphorylation levels, which has been reported to inhibit Ca(2+) pump activity, whereas in contrast, BK stimulates a reduction of PMCA tyrosine phosphorylation levels. Carbachol 114-117 ATPase plasma membrane Ca2+ transporting 2 Homo sapiens 284-288 12594217-10 2003 Thus, BK and CCh have a differential effect both on Ca(2+) pump activity and on tyrosine phosphorylation levels of the PMCA. Carbachol 13-16 ATPase plasma membrane Ca2+ transporting 2 Homo sapiens 119-123 12609749-6 2003 Hence, low concentrations of IAPP brought about a modest increase of basal insulin secretion at 7 mM glucose and also of insulin release stimulated by carbachol. Carbachol 151-160 islet amyloid polypeptide Homo sapiens 29-33 12628460-4 2003 Both CCh and PBr increased the relative amounts of Dsg 1 and Dsg 3. Carbachol 5-8 desmoglein 1 Homo sapiens 51-56 12628460-4 2003 Both CCh and PBr increased the relative amounts of Dsg 1 and Dsg 3. Carbachol 5-8 desmoglein 3 Homo sapiens 61-66 12628460-7 2003 The Atr-dependent phosphorylation of Dsg 3 was inhibited in the presence of 0.5 mM CCh. Carbachol 83-86 desmoglein 3 Homo sapiens 37-42 12646175-5 2003 The neurotransmitters, which were implicated in sleep/wake regulation, affected the activity of orexin neurons; noradrenaline and serotonin hyperpolarized, while carbachol depolarized orexin neurons in either the presence or absence of tetrodotoxin. Carbachol 162-171 hypocretin Mus musculus 96-102 12646175-5 2003 The neurotransmitters, which were implicated in sleep/wake regulation, affected the activity of orexin neurons; noradrenaline and serotonin hyperpolarized, while carbachol depolarized orexin neurons in either the presence or absence of tetrodotoxin. Carbachol 162-171 hypocretin Mus musculus 184-190 12460119-2 2003 Exposure to carbachol elicited transient translocation of PKC alpha-EGFP and beta II-EGFP in most of the cells, PKC delta-EGFP in a few cells and induced sustained translocation of PKC epsilon-EGFP. Carbachol 12-21 protein kinase C alpha Homo sapiens 58-67 12460119-2 2003 Exposure to carbachol elicited transient translocation of PKC alpha-EGFP and beta II-EGFP in most of the cells, PKC delta-EGFP in a few cells and induced sustained translocation of PKC epsilon-EGFP. Carbachol 12-21 protein kinase C delta Homo sapiens 112-121 12460119-8 2003 Experiments with individual C1 domains showed that treatment with carbachol or phorbol 12,13-dibutyrate elicited translocation of PKC alpha C1a, PKC epsilon C1a and PKC epsilon C1b, whereas PKC alpha C1b was largely insensitive to these agents. Carbachol 66-75 protein kinase C alpha Homo sapiens 130-139 12460119-8 2003 Experiments with individual C1 domains showed that treatment with carbachol or phorbol 12,13-dibutyrate elicited translocation of PKC alpha C1a, PKC epsilon C1a and PKC epsilon C1b, whereas PKC alpha C1b was largely insensitive to these agents. Carbachol 66-75 endogenous retrovirus group K member 1 Homo sapiens 140-143 12460119-8 2003 Experiments with individual C1 domains showed that treatment with carbachol or phorbol 12,13-dibutyrate elicited translocation of PKC alpha C1a, PKC epsilon C1a and PKC epsilon C1b, whereas PKC alpha C1b was largely insensitive to these agents. Carbachol 66-75 proline rich transmembrane protein 2 Homo sapiens 130-133 12460119-9 2003 In contrast with full-length PKC alpha, the regulatory domain of PKC alpha and pseudosubstrate-devoid PKC alpha responded to the carbachol-stimulated increase in diacylglycerol. Carbachol 129-138 protein kinase C alpha Homo sapiens 65-74 12460119-9 2003 In contrast with full-length PKC alpha, the regulatory domain of PKC alpha and pseudosubstrate-devoid PKC alpha responded to the carbachol-stimulated increase in diacylglycerol. Carbachol 129-138 protein kinase C alpha Homo sapiens 65-74 12612139-9 2003 In conclusion, a reduced expression of PKCalpha and PLCbeta(1) may be involved in the decreased insulin secretion by islets from LP rats after stimulation with CCh and PMA. Carbachol 160-163 protein kinase C, alpha Rattus norvegicus 39-47 12612139-9 2003 In conclusion, a reduced expression of PKCalpha and PLCbeta(1) may be involved in the decreased insulin secretion by islets from LP rats after stimulation with CCh and PMA. Carbachol 160-163 phospholipase C beta 1 Rattus norvegicus 52-62 12494403-4 2003 An in vitro electrophysiological study investigated the effect of isolation rearing on postsynaptic 5-HT(1A) function on CA1 hippocampal neurones activated with the muscarinic agonist carbachol and found no change in the sensitivity of these postsynaptic receptors between the groups. Carbachol 184-193 carbonic anhydrase 1 Rattus norvegicus 121-124 12388102-8 2003 When cells were pretreated with SB-203580 and PD-98059 to simultaneously inhibit p38 and ERK MAPKs, respectively, I(sc) responses to TG and CCh were significantly greater than those observed with either inhibitor alone. Carbachol 140-143 mitogen-activated protein kinase 14 Homo sapiens 81-84 12797549-4 2003 Both IFNgamma and carbachol triggered IFNgamma secretion in SMG. Carbachol 18-27 interferon gamma Mus musculus 38-46 12797549-7 2003 Moreover, cyclooxygenase-2 (COX-2) activation and subsequent PGE2 liberation, in a nitric oxide independent manner, seem to be involved in M3 and M2 receptor activation by carbachol. Carbachol 172-181 prostaglandin-endoperoxide synthase 2 Mus musculus 10-26 12797549-7 2003 Moreover, cyclooxygenase-2 (COX-2) activation and subsequent PGE2 liberation, in a nitric oxide independent manner, seem to be involved in M3 and M2 receptor activation by carbachol. Carbachol 172-181 prostaglandin-endoperoxide synthase 2 Mus musculus 28-33 12831623-0 2003 [Effects of carbachol on plasma levels of tumor necrosis factor-alpha and interleukin-10 in rats during gut ischemia-reperfusion]. Carbachol 12-21 tumor necrosis factor Rattus norvegicus 42-69 12831623-0 2003 [Effects of carbachol on plasma levels of tumor necrosis factor-alpha and interleukin-10 in rats during gut ischemia-reperfusion]. Carbachol 12-21 interleukin 10 Rattus norvegicus 74-88 12831623-1 2003 OBJECTIVE: To investigate the effects of carbachol on the levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-10 (IL-10) and cortisol in plasma of rats during gut ischemia-reperfusion. Carbachol 41-50 tumor necrosis factor Rattus norvegicus 68-95 12831623-1 2003 OBJECTIVE: To investigate the effects of carbachol on the levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-10 (IL-10) and cortisol in plasma of rats during gut ischemia-reperfusion. Carbachol 41-50 tumor necrosis factor Rattus norvegicus 97-106 12831623-1 2003 OBJECTIVE: To investigate the effects of carbachol on the levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-10 (IL-10) and cortisol in plasma of rats during gut ischemia-reperfusion. Carbachol 41-50 interleukin 10 Rattus norvegicus 109-123 12831623-1 2003 OBJECTIVE: To investigate the effects of carbachol on the levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-10 (IL-10) and cortisol in plasma of rats during gut ischemia-reperfusion. Carbachol 41-50 interleukin 10 Rattus norvegicus 125-130 12831623-6 2003 The levels of TNF-alpha significantly decreased in pretreated and treated groups than that in control after the intramuscular injection of carbachol (all P<0.01). Carbachol 139-148 tumor necrosis factor Rattus norvegicus 14-23 12609749-7 2003 High concentrations of IAPP, however, inhibited insulin release stimulated by glucose (10 and 16.7 mM), IBMX, carbachol and L-arginine. Carbachol 110-119 islet amyloid polypeptide Homo sapiens 23-27 12609749-7 2003 High concentrations of IAPP, however, inhibited insulin release stimulated by glucose (10 and 16.7 mM), IBMX, carbachol and L-arginine. Carbachol 110-119 insulin Homo sapiens 48-55 12388102-3 2003 Western blot analysis of T(84) colonic epithelial cells revealed that the muscarinic agonist carbachol (CCh; 100 microM) stimulated phosphorylation and activation of p38 MAPK. Carbachol 93-102 mitogen-activated protein kinase 14 Homo sapiens 166-169 12388102-3 2003 Western blot analysis of T(84) colonic epithelial cells revealed that the muscarinic agonist carbachol (CCh; 100 microM) stimulated phosphorylation and activation of p38 MAPK. Carbachol 104-107 mitogen-activated protein kinase 14 Homo sapiens 166-169 12388102-4 2003 The p38 inhibitor SB-203580 (10 microM) potentiated and prolonged short-circuit current (I(sc)) responses to CCh across voltage-clamped T(84) cells to 157.4 +/- 6.9% of those in control cells (n = 21; P < 0.001). Carbachol 109-112 mitogen-activated protein kinase 14 Homo sapiens 4-7 12388102-5 2003 CCh-induced p38 phosphorylation was attenuated by the EGFR inhibitor tyrphostin AG-1478 (0.1 nM-10 microM) and by the Src family kinase inhibitor PP2 (20 nM-2 microM). Carbachol 0-3 mitogen-activated protein kinase 14 Homo sapiens 12-15 12388102-5 2003 CCh-induced p38 phosphorylation was attenuated by the EGFR inhibitor tyrphostin AG-1478 (0.1 nM-10 microM) and by the Src family kinase inhibitor PP2 (20 nM-2 microM). Carbachol 0-3 epidermal growth factor receptor Homo sapiens 54-58 12388102-5 2003 CCh-induced p38 phosphorylation was attenuated by the EGFR inhibitor tyrphostin AG-1478 (0.1 nM-10 microM) and by the Src family kinase inhibitor PP2 (20 nM-2 microM). Carbachol 0-3 neuropeptide Y receptor Y6 (pseudogene) Homo sapiens 146-149 12388102-6 2003 The effects of CCh on p38 phosphorylation were mimicked by thapsigargin (TG; 2 microM), which specifically elevates intracellular Ca(2+), and were abolished by the Ca(2+) chelator BAPTA-AM (20 microM), implying a role for intracellular Ca(2+) in mediating p38 activation. Carbachol 15-18 mitogen-activated protein kinase 14 Homo sapiens 22-25 12388102-6 2003 The effects of CCh on p38 phosphorylation were mimicked by thapsigargin (TG; 2 microM), which specifically elevates intracellular Ca(2+), and were abolished by the Ca(2+) chelator BAPTA-AM (20 microM), implying a role for intracellular Ca(2+) in mediating p38 activation. Carbachol 15-18 mitogen-activated protein kinase 14 Homo sapiens 256-259 12388397-3 2003 Stimulation with carbachol or ATP decreased initial uptake by 44 +/- 3 (n = 14) and 34 +/- 4% (n = 21), respectively, independently of PKC but dependent on phosphatidylinositol 3-kinase (PI3K). Carbachol 17-26 phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta Homo sapiens 156-185 12610653-6 2003 In contrast, carbachol increases the secretion of sAPPalpha to similar levels in wild-type cells and in cells transfected with antisense PKCalpha by acting on APP metabolism through an indirect pathway partially involving the activation of PKC. Carbachol 13-22 protein kinase C alpha Homo sapiens 137-145 12610653-6 2003 In contrast, carbachol increases the secretion of sAPPalpha to similar levels in wild-type cells and in cells transfected with antisense PKCalpha by acting on APP metabolism through an indirect pathway partially involving the activation of PKC. Carbachol 13-22 protein kinase C alpha Homo sapiens 137-140 12524182-4 2003 In young adulthood (50 days old), control offspring showed an increase in hippocampal cell membrane PKCgamma after incubation with the muscarinic cholinergic receptor agonist, carbachol, indicative of translocation from the cytosol. Carbachol 176-185 protein kinase C, gamma Mus musculus 100-108 12388118-5 2003 Inhibition of EGF receptor (EGFR) with AG1478, an inhibitor of the EGFR tyrosine kinase activity, significantly increased phenylephrine- but not carbachol-induced protein secretion. Carbachol 145-154 epidermal growth factor receptor Rattus norvegicus 28-32 12388118-7 2003 Phenylephrine stimulated tyrosine phosphorylation of the EGFR, whereas carbachol stimulated p60(Src), and possibly Pyk2, to activate MAPK. Carbachol 71-80 SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus 96-99 12388102-10 2003 CCh-stimulated p38 activation constitutes a similar, but distinct and complementary, antisecretory signaling pathway to that of ERK MAPK. Carbachol 0-3 mitogen-activated protein kinase 14 Homo sapiens 15-18 12388118-7 2003 Phenylephrine stimulated tyrosine phosphorylation of the EGFR, whereas carbachol stimulated p60(Src), and possibly Pyk2, to activate MAPK. Carbachol 71-80 protein tyrosine kinase 2 beta Rattus norvegicus 115-119 12388102-10 2003 CCh-stimulated p38 activation constitutes a similar, but distinct and complementary, antisecretory signaling pathway to that of ERK MAPK. Carbachol 0-3 mitogen-activated protein kinase 1 Homo sapiens 128-131 12388102-10 2003 CCh-stimulated p38 activation constitutes a similar, but distinct and complementary, antisecretory signaling pathway to that of ERK MAPK. Carbachol 0-3 mitogen-activated protein kinase 1 Homo sapiens 132-136 12522076-1 2003 1 In fura 2-loaded HEK-293 cells stably expressing human type 1 parathyroid hormone (PTH) receptors, PTH potentiated the Ca(2+) mobilization evoked by carbachol by >4 fold without itself increasing the intracellular [Ca(2+)]. Carbachol 151-160 parathyroid hormone Homo sapiens 64-83 12388338-2 2003 Allergen challenge significantly reduced the relaxant effect of salbutamol on carbachol-induced contractions, suggesting beta(2)-adrenoceptor (beta(2)-AR) pathway dysfunction. Carbachol 78-87 adrenoceptor beta 2 Homo sapiens 121-141 12388338-2 2003 Allergen challenge significantly reduced the relaxant effect of salbutamol on carbachol-induced contractions, suggesting beta(2)-adrenoceptor (beta(2)-AR) pathway dysfunction. Carbachol 78-87 adrenoceptor beta 2 Homo sapiens 143-153 12388338-4 2003 Incubation with the G(s)alpha protein-stimulating cholera toxin attenuated contractile responses to carbachol significantly less in challenged than in unchallenged rings. Carbachol 100-109 GNAS complex locus Homo sapiens 20-29 12522076-1 2003 1 In fura 2-loaded HEK-293 cells stably expressing human type 1 parathyroid hormone (PTH) receptors, PTH potentiated the Ca(2+) mobilization evoked by carbachol by >4 fold without itself increasing the intracellular [Ca(2+)]. Carbachol 151-160 parathyroid hormone Homo sapiens 85-88 12522076-1 2003 1 In fura 2-loaded HEK-293 cells stably expressing human type 1 parathyroid hormone (PTH) receptors, PTH potentiated the Ca(2+) mobilization evoked by carbachol by >4 fold without itself increasing the intracellular [Ca(2+)]. Carbachol 151-160 parathyroid hormone Homo sapiens 101-104 12498921-0 2003 The indirect negative inotropic effect of carbachol in beta1-adrenoceptor antagonist-treated human right atria. Carbachol 42-51 adrenoceptor beta 1 Homo sapiens 55-73 12498921-4 2003 pD(2) values for carbachol, however, were higher in atria from non-beta(1)-adrenoceptor antagonist-treated vs. beta(1)-adrenoceptor antagonist-treated patients.We conclude that, in isolated human right atria, carbachol-induced indirect negative inotropic effect is not dependent from the agonist employed to increase (via cyclic AMP accumulation) contractile force. Carbachol 17-26 adrenoceptor beta 1 Homo sapiens 67-87 12498921-4 2003 pD(2) values for carbachol, however, were higher in atria from non-beta(1)-adrenoceptor antagonist-treated vs. beta(1)-adrenoceptor antagonist-treated patients.We conclude that, in isolated human right atria, carbachol-induced indirect negative inotropic effect is not dependent from the agonist employed to increase (via cyclic AMP accumulation) contractile force. Carbachol 17-26 adrenoceptor beta 1 Homo sapiens 111-131 12498921-5 2003 However, in atria from beta(1)-adrenoceptor antagonist-treated patients, carbachol-induced indirect negative inotropic effect is attenuated. Carbachol 73-82 adrenoceptor beta 1 Homo sapiens 23-43 12768388-0 2003 Relaxation effects of adrenomedullin in carbachol-precontracted rabbit internal anal sphincter. Carbachol 40-49 ADM Oryctolagus cuniculus 22-36 12576703-3 2003 In measurements of the relationship between [Ca(2+)](i) and tension in intact tissue, Y-27632, a ROK inhibitor, significantly attenuated the carbachol-induced contraction without changing [Ca (2+)](i). Carbachol 141-150 Rho-associated coiled-coil containing protein kinase 2 Rattus norvegicus 97-100 12598604-4 2003 Activation of muscarinic acetylcholine receptors (mAChRs) with carbachol produced an enhancement of GABA(A) receptor currents in acutely dissociated cells after a short treatment with insulin. Carbachol 63-72 insulin Homo sapiens 184-191 12598604-5 2003 Inhibiting phosphoinositide-3 kinase (PI3K), a downstream target of insulin signaling, eliminated this effect as well as the carbachol-induced enhancement of GABAergic miniature IPSC amplitudes in PFC slices. Carbachol 125-134 phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta Homo sapiens 11-36 12598604-5 2003 Inhibiting phosphoinositide-3 kinase (PI3K), a downstream target of insulin signaling, eliminated this effect as well as the carbachol-induced enhancement of GABAergic miniature IPSC amplitudes in PFC slices. Carbachol 125-134 insulin Homo sapiens 68-75 12576703-4 2003 Phosphorylation of MLC(20) was increased by carbachol and this increased phosphorylation was blocked by treatment of tissue with Y-27632. Carbachol 44-53 myosin light chain 12B Rattus norvegicus 19-26 12763063-3 2003 We found that carbachol diminished excitatory post-synaptic responses induced by CA1 stratum radiatum stimulation in wild type mice, but caused an unexpected increase in knockout animals. Carbachol 14-23 carbonic anhydrase 1 Mus musculus 81-84 12927200-5 2003 Carbachol activated NF-kappaB in human 1321N1 astrocytoma cells, as evidenced by translocation of the p65 subunit of NF-kappaB to the nucleus, phosphorylation and degradation of IkappaBalpha in the cytosol, and increase NF-kappaB binding to DNA. Carbachol 0-9 nuclear factor kappa B subunit 1 Homo sapiens 20-29 12927200-5 2003 Carbachol activated NF-kappaB in human 1321N1 astrocytoma cells, as evidenced by translocation of the p65 subunit of NF-kappaB to the nucleus, phosphorylation and degradation of IkappaBalpha in the cytosol, and increase NF-kappaB binding to DNA. Carbachol 0-9 RELA proto-oncogene, NF-kB subunit Homo sapiens 102-105 12927200-5 2003 Carbachol activated NF-kappaB in human 1321N1 astrocytoma cells, as evidenced by translocation of the p65 subunit of NF-kappaB to the nucleus, phosphorylation and degradation of IkappaBalpha in the cytosol, and increase NF-kappaB binding to DNA. Carbachol 0-9 nuclear factor kappa B subunit 1 Homo sapiens 117-126 12927200-5 2003 Carbachol activated NF-kappaB in human 1321N1 astrocytoma cells, as evidenced by translocation of the p65 subunit of NF-kappaB to the nucleus, phosphorylation and degradation of IkappaBalpha in the cytosol, and increase NF-kappaB binding to DNA. Carbachol 0-9 NFKB inhibitor alpha Homo sapiens 178-190 12927200-5 2003 Carbachol activated NF-kappaB in human 1321N1 astrocytoma cells, as evidenced by translocation of the p65 subunit of NF-kappaB to the nucleus, phosphorylation and degradation of IkappaBalpha in the cytosol, and increase NF-kappaB binding to DNA. Carbachol 0-9 nuclear factor kappa B subunit 1 Homo sapiens 117-126 12927200-6 2003 Carbachol also induced translocation of p65 to the nucleus in primary rat astrocytes. Carbachol 0-9 synaptotagmin 1 Rattus norvegicus 40-43 12927200-7 2003 Carbachol-induced NF-kappaB activation was mediated by the M3 subtype of muscarinic receptors and appeared to involve Ca(2+) mobilization and activation of PKC epsilon and PKC zeta, but not PI3-kinase and mitogen-activated protein kinase. Carbachol 0-9 nuclear factor kappa B subunit 1 Homo sapiens 18-27 12927200-8 2003 The NF-kappaB peptide inhibitor SN50, but not the inactive peptide SN50M, strongly inhibited carbachol-induced astrocytoma cells proliferation and p65 translocation to the nucleus. Carbachol 93-102 nuclear factor kappa B subunit 1 Homo sapiens 4-13 12927200-11 2003 Together, these results suggest that activation of NF-kappaB by muscarinic receptors in astroglial cells is important for carbachol-induced DNA synthesis and that ethanol-mediated inhibition of cell proliferation may be due in part to inhibition of NF-kappaB activation. Carbachol 122-131 nuclear factor kappa B subunit 1 Homo sapiens 51-60 12518226-4 2003 In the present study, it was found that carbachol not only activated MEK/ERK-1/2 signaling pathways, but also increased the expression levels of Bcl-2 and phospho-Bad proteins in human neuroblastoma SH-SY5Y cells. Carbachol 40-49 mitogen-activated protein kinase kinase 7 Homo sapiens 69-72 12518226-4 2003 In the present study, it was found that carbachol not only activated MEK/ERK-1/2 signaling pathways, but also increased the expression levels of Bcl-2 and phospho-Bad proteins in human neuroblastoma SH-SY5Y cells. Carbachol 40-49 mitogen-activated protein kinase 3 Homo sapiens 73-80 12518226-4 2003 In the present study, it was found that carbachol not only activated MEK/ERK-1/2 signaling pathways, but also increased the expression levels of Bcl-2 and phospho-Bad proteins in human neuroblastoma SH-SY5Y cells. Carbachol 40-49 BCL2 apoptosis regulator Homo sapiens 145-150 12518226-6 2003 Furthermore, carbachol also stimulated Bcl-2 promoter-driven luciferase gene expression in transfected SH-SY5Y cells. Carbachol 13-22 BCL2 apoptosis regulator Homo sapiens 39-44 12368283-11 2002 Finally, in situ phosphorylation assays demonstrated that PMCA was phosphorylated by treatment with forskolin but only in the presence of carbamylcholine (carbachol). Carbachol 138-153 ATPase plasma membrane Ca2+ transporting 2 Homo sapiens 58-62 12368283-11 2002 Finally, in situ phosphorylation assays demonstrated that PMCA was phosphorylated by treatment with forskolin but only in the presence of carbamylcholine (carbachol). Carbachol 155-164 ATPase plasma membrane Ca2+ transporting 2 Homo sapiens 58-62 12368284-11 2002 Transient expression of the hTRPC3 protein enhanced Ca(2+) influx responsive to carbachol but did not increase EGF-activated Ca(2+) influx. Carbachol 80-89 transient receptor potential cation channel subfamily C member 3 Homo sapiens 28-34 12374786-6 2002 The loss of Kir3.1 did not affect the level of atrial Kir3.4 protein but was correlated with a loss of carbachol-induced current in atrial myocytes. Carbachol 103-112 potassium inwardly-rectifying channel, subfamily J, member 3 Mus musculus 12-18 12388311-9 2002 MAP 4 protein decorated the microtubules extensively, and receptor recovery upon carbachol withdrawal was reduced to 54% of control. Carbachol 81-90 microtubule associated protein 4 Homo sapiens 0-5 12490593-2 2003 Magnitudes of acute, nAChR-mediated, specific 86Rb+ efflux responses to 1 mM carbamylcholine were reduced after pretreatment with specific nAChR ligands in effects that depended on pretreatment drug dose, duration of drug pretreatment, and duration of drug-free recovery. Carbachol 77-92 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 21-26 12490593-2 2003 Magnitudes of acute, nAChR-mediated, specific 86Rb+ efflux responses to 1 mM carbamylcholine were reduced after pretreatment with specific nAChR ligands in effects that depended on pretreatment drug dose, duration of drug pretreatment, and duration of drug-free recovery. Carbachol 77-92 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 139-144 12185080-7 2002 However, eNOS was tightly coupled with caveolin-1, and was dissociated from heat shock protein 90 or calmodulin in the hypoxic pulmonary artery in either the presence or absence of carbachol. Carbachol 181-190 nitric oxide synthase 3 Rattus norvegicus 9-13 12429569-12 2002 Culture with TNFalpha produced a time- and concentration-dependent increase in the maximal contraction to 5-HT, evidently mediated by 5-HT(2A) receptors, whereas, the potency for carbachol was reduced. Carbachol 179-188 tumor necrosis factor Mus musculus 13-21 12492954-7 2002 The CCh-induced outward current was inhibited by iberiotoxin, a selective inhibitor of large-conductance Ca2+-activated K+ channels (BKCa). Carbachol 4-7 potassium calcium-activated channel subfamily M alpha 1 Rattus norvegicus 133-137 12492954-8 2002 CONCLUSION: CCh induces BKCa, which is inhibited by M2- and Gi-mediated signal transduction pathway. Carbachol 12-15 potassium calcium-activated channel subfamily M alpha 1 Rattus norvegicus 24-28 12372816-7 2002 The PKCepsilon-selective agonist carbachol (100 microM) induced prolonged stimulation of endocytosis devoid of an inhibitory phase. Carbachol 33-42 protein kinase C epsilon Homo sapiens 4-14 12202486-9 2002 A metalloproteinase inhibitor, WAY171318, reduced CCh-induced phosphorylation of ERK and completely blocked EGFr phosphorylation and TGF-alpha release. Carbachol 50-53 mitogen-activated protein kinase 1 Homo sapiens 81-84 12202486-10 2002 We conclude that CCh-stimulated EGFr transactivation and subsequent ERK activation, a pathway that limits CCh-induced chloride secretion, is mediated by metalloproteinase-dependent extracellular release of TGF-alpha and intracellular Src activation. Carbachol 17-20 epidermal growth factor receptor Homo sapiens 32-36 12438084-10 2002 By contrast, while muscarine, nicotine, or carbachol (100 micro M) also evoke rapid increases in rat PNMT promoter activity, peak activity is observed at 6 hours, followed by a decline and restoration to basal levels by 24 hours. Carbachol 43-52 phenylethanolamine-N-methyltransferase Rattus norvegicus 101-105 12202486-10 2002 We conclude that CCh-stimulated EGFr transactivation and subsequent ERK activation, a pathway that limits CCh-induced chloride secretion, is mediated by metalloproteinase-dependent extracellular release of TGF-alpha and intracellular Src activation. Carbachol 17-20 mitogen-activated protein kinase 1 Homo sapiens 68-71 12202486-10 2002 We conclude that CCh-stimulated EGFr transactivation and subsequent ERK activation, a pathway that limits CCh-induced chloride secretion, is mediated by metalloproteinase-dependent extracellular release of TGF-alpha and intracellular Src activation. Carbachol 17-20 transforming growth factor alpha Homo sapiens 206-215 12202486-10 2002 We conclude that CCh-stimulated EGFr transactivation and subsequent ERK activation, a pathway that limits CCh-induced chloride secretion, is mediated by metalloproteinase-dependent extracellular release of TGF-alpha and intracellular Src activation. Carbachol 106-109 epidermal growth factor receptor Homo sapiens 32-36 12202486-10 2002 We conclude that CCh-stimulated EGFr transactivation and subsequent ERK activation, a pathway that limits CCh-induced chloride secretion, is mediated by metalloproteinase-dependent extracellular release of TGF-alpha and intracellular Src activation. Carbachol 106-109 mitogen-activated protein kinase 1 Homo sapiens 68-71 12149271-7 2002 We found that perfused Sur1 null pancreata secreted insulin in response to the cholinergic agonist carbachol in a glucose-dependent manner. Carbachol 99-108 ATP-binding cassette, sub-family C (CFTR/MRP), member 8 Mus musculus 23-27 12223352-6 2002 However, whereas IFN-gamma significantly inhibited carbachol-induced Cl(-) secretion, neither neutralizing antibodies to TGF-alpha nor an EGFr inhibitor (1 microM tyrphostin AG 1478) were able to reverse this inhibitory effect. Carbachol 51-60 interferon gamma Homo sapiens 17-26 14566596-7 2002 Combination of CCh and ISP in different concentrations resulted in distinctive morphological changes which reflect fluid secretion and mucin secretion. Carbachol 15-18 solute carrier family 13 member 2 Rattus norvegicus 135-140 12202486-0 2002 Transactivation of the epidermal growth factor receptor in colonic epithelial cells by carbachol requires extracellular release of transforming growth factor-alpha. Carbachol 87-96 epidermal growth factor receptor Homo sapiens 23-55 12202486-0 2002 Transactivation of the epidermal growth factor receptor in colonic epithelial cells by carbachol requires extracellular release of transforming growth factor-alpha. Carbachol 87-96 tumor necrosis factor Homo sapiens 131-163 12202486-1 2002 We have shown previously that the muscarinic agonist, carbachol (CCh), transactivates the epidermal growth factor receptor (EGFr) via calmodulin, Pyk-2, and Src kinase activation. Carbachol 54-63 epidermal growth factor receptor Homo sapiens 90-122 12202486-1 2002 We have shown previously that the muscarinic agonist, carbachol (CCh), transactivates the epidermal growth factor receptor (EGFr) via calmodulin, Pyk-2, and Src kinase activation. Carbachol 54-63 epidermal growth factor receptor Homo sapiens 124-128 12202486-1 2002 We have shown previously that the muscarinic agonist, carbachol (CCh), transactivates the epidermal growth factor receptor (EGFr) via calmodulin, Pyk-2, and Src kinase activation. Carbachol 54-63 protein tyrosine kinase 2 beta Homo sapiens 146-151 12202486-1 2002 We have shown previously that the muscarinic agonist, carbachol (CCh), transactivates the epidermal growth factor receptor (EGFr) via calmodulin, Pyk-2, and Src kinase activation. Carbachol 65-68 epidermal growth factor receptor Homo sapiens 90-122 12202486-1 2002 We have shown previously that the muscarinic agonist, carbachol (CCh), transactivates the epidermal growth factor receptor (EGFr) via calmodulin, Pyk-2, and Src kinase activation. Carbachol 65-68 epidermal growth factor receptor Homo sapiens 124-128 12202486-1 2002 We have shown previously that the muscarinic agonist, carbachol (CCh), transactivates the epidermal growth factor receptor (EGFr) via calmodulin, Pyk-2, and Src kinase activation. Carbachol 65-68 protein tyrosine kinase 2 beta Homo sapiens 146-151 12202486-2 2002 EGFr phosphorylation causes extracellular signal-regulated kinase (ERK) activation and inhibits CCh-stimulated chloride secretion across intestinal epithelial cells. Carbachol 96-99 epidermal growth factor receptor Homo sapiens 0-4 12202486-3 2002 Here we investigated whether CCh-stimulated EGFr transactivation involves EGFr ligand release. Carbachol 29-32 epidermal growth factor receptor Homo sapiens 44-48 12202486-3 2002 Here we investigated whether CCh-stimulated EGFr transactivation involves EGFr ligand release. Carbachol 29-32 epidermal growth factor receptor Homo sapiens 74-78 12202486-4 2002 Pre-incubation of T(84) cell monolayers with a neutralizing antibody to the EGFr ligand binding domain decreased CCh-induced phosphorylation of EGFr and ERK. Carbachol 113-116 epidermal growth factor receptor Homo sapiens 76-80 12202486-4 2002 Pre-incubation of T(84) cell monolayers with a neutralizing antibody to the EGFr ligand binding domain decreased CCh-induced phosphorylation of EGFr and ERK. Carbachol 113-116 epidermal growth factor receptor Homo sapiens 144-148 12202486-4 2002 Pre-incubation of T(84) cell monolayers with a neutralizing antibody to the EGFr ligand binding domain decreased CCh-induced phosphorylation of EGFr and ERK. Carbachol 113-116 mitogen-activated protein kinase 1 Homo sapiens 153-156 12202486-5 2002 CCh-stimulated efflux of (86)Rb+ from T(84) cell monolayers, which parallels changes in chloride secretion, was potentiated by anti-EGFr pre-incubation. Carbachol 0-3 epidermal growth factor receptor Homo sapiens 132-136 12202486-7 2002 Co-incubation with the Src kinase inhibitor PP2 and anti-EGFr had an additive inhibitory effect on CCh-induced ERK phosphorylation greater than either inhibitor alone. Carbachol 99-102 neuropeptide Y receptor Y6 (pseudogene) Homo sapiens 44-47 12202486-7 2002 Co-incubation with the Src kinase inhibitor PP2 and anti-EGFr had an additive inhibitory effect on CCh-induced ERK phosphorylation greater than either inhibitor alone. Carbachol 99-102 epidermal growth factor receptor Homo sapiens 57-61 12202486-7 2002 Co-incubation with the Src kinase inhibitor PP2 and anti-EGFr had an additive inhibitory effect on CCh-induced ERK phosphorylation greater than either inhibitor alone. Carbachol 99-102 mitogen-activated protein kinase 1 Homo sapiens 111-114 12202486-8 2002 CCh caused the basolateral release of transforming growth factor alpha (TGF-alpha) into T(84) cell bathing media. Carbachol 0-3 tumor necrosis factor Homo sapiens 38-70 12202486-8 2002 CCh caused the basolateral release of transforming growth factor alpha (TGF-alpha) into T(84) cell bathing media. Carbachol 0-3 transforming growth factor alpha Homo sapiens 72-81 12370534-0 2002 Regulation of carbachol-induced c-fos mRNA expression in AR42J cells by somatostatin receptor subtypes 1, 2, and 3. Carbachol 14-23 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 32-37 12370534-0 2002 Regulation of carbachol-induced c-fos mRNA expression in AR42J cells by somatostatin receptor subtypes 1, 2, and 3. Carbachol 14-23 somatostatin receptor 1 Rattus norvegicus 72-114 12370534-5 2002 When AR42J cells were exposed to the cholinergic agonist carbachol in the presence of somatostatin or selective SSTR agonists, significant and dose-dependent reductions in agonist-induced levels of mRNA were noted. Carbachol 57-66 somatostatin receptor 3 Rattus norvegicus 112-116 12370534-9 2002 CONCLUSION: The current studies demonstrate that somatostatin inhibits carbachol-induced increases in expression by interacting with somatostatin receptor subtypes 1, 2, and 3. Carbachol 71-80 somatostatin receptor 1 Rattus norvegicus 133-175 12121968-1 2002 We studied effects of the familial Alzheimer"s disease presenilin 1 (PS1) exon 9 deletion (PS1-DeltaE9) mutation on basal and carbachol-stimulated phosphoinositide (PI) hydrolysis and intracellular Ca(2+) concentrations ([Ca(2+)](i)) in human SH-SY5Y neuroblastoma cells. Carbachol 126-135 presenilin 1 Homo sapiens 55-67 12121968-1 2002 We studied effects of the familial Alzheimer"s disease presenilin 1 (PS1) exon 9 deletion (PS1-DeltaE9) mutation on basal and carbachol-stimulated phosphoinositide (PI) hydrolysis and intracellular Ca(2+) concentrations ([Ca(2+)](i)) in human SH-SY5Y neuroblastoma cells. Carbachol 126-135 presenilin 1 Homo sapiens 69-72 12121968-5 2002 Carbachol gave a greater stimulation of [Ca(2+)](i) in PS1-DeltaE9 cells that took longer to return to basal as compared with responses seen in NT and PS1-WT cells. Carbachol 0-9 presenilin 1 Homo sapiens 55-58 12121968-6 2002 This long tail-off effect seen in PS1-DeltaE9 cells after carbachol stimulation was reversed by xestospongin C and dantrolene, suggesting that it was mediated by inositol trisphosphate receptor and ryanodine receptor amplification of Ca(2+). Carbachol 58-67 presenilin 1 Homo sapiens 34-37 12372560-1 2002 Phase and amplitude depth profiles of EEG theta-like activity (TLA) induced by bath perfusion of 50 micro M of the cholinergic agonist carbachol were investigated using rat hippocampal formation slices. Carbachol 135-144 RT1 class Ib, locus T18 Rattus norvegicus 63-66 12213302-0 2002 Neuropeptide Y potentiates the pressor response evoked by carbachol administration into the posterior hypothalamic nucleus of conscious rat. Carbachol 58-67 neuropeptide Y Rattus norvegicus 0-14 12213302-6 2002 Administration of 0.23 nmol of NPY 60 min prior to CCh induced a parallel shift to the left of the dose-response curves for CCh resulting in an increase in relative potency for CCh of 6.4 to 6.9 times. Carbachol 51-54 neuropeptide Y Rattus norvegicus 31-34 12213302-6 2002 Administration of 0.23 nmol of NPY 60 min prior to CCh induced a parallel shift to the left of the dose-response curves for CCh resulting in an increase in relative potency for CCh of 6.4 to 6.9 times. Carbachol 124-127 neuropeptide Y Rattus norvegicus 31-34 12213302-6 2002 Administration of 0.23 nmol of NPY 60 min prior to CCh induced a parallel shift to the left of the dose-response curves for CCh resulting in an increase in relative potency for CCh of 6.4 to 6.9 times. Carbachol 124-127 neuropeptide Y Rattus norvegicus 31-34 12213302-7 2002 This NPY-mediated enhancement was prevented by pretreatment with PYX-2 which alone did not affect the CCh-induced pressor response. Carbachol 102-105 neuropeptide Y Rattus norvegicus 5-8 12213302-8 2002 These results show that NPY enhances the pressor response evoked by CCh administration into the PHN of the conscious rat and that this enhancement is mediated by stimulation of a Y receptor. Carbachol 68-71 neuropeptide Y Rattus norvegicus 24-27 12231388-3 2002 PNU-171990 caused a parallel shift in the concentration-response curve for carbachol-induced contraction of smooth muscle from guinea pig bladder (pK(B), 7.65), guinea pig ileum (pK(B), 8.48), and human ileum (pK(B), 7.10). Carbachol 75-84 AKT serine/threonine kinase 1 Homo sapiens 147-151 12231388-3 2002 PNU-171990 caused a parallel shift in the concentration-response curve for carbachol-induced contraction of smooth muscle from guinea pig bladder (pK(B), 7.65), guinea pig ileum (pK(B), 8.48), and human ileum (pK(B), 7.10). Carbachol 75-84 AKT serine/threonine kinase 1 Homo sapiens 179-183 12231388-3 2002 PNU-171990 caused a parallel shift in the concentration-response curve for carbachol-induced contraction of smooth muscle from guinea pig bladder (pK(B), 7.65), guinea pig ileum (pK(B), 8.48), and human ileum (pK(B), 7.10). Carbachol 75-84 AKT serine/threonine kinase 1 Homo sapiens 179-183 12128250-1 2002 The activation of muscle PKC isozymes following treatment with carbachol, an acetylcholine receptor agonist, has been investigated. Carbachol 63-72 protein kinase C, alpha Mus musculus 25-28 12369741-5 2002 The present study sought to determine 1) the functional selectivity of carbachol for cholinergic muscarinic and/or nicotinic receptors involved in the stimulation of HPA axis; 2) the involvement of prostaglandins (PGs) generated by constitutive and inducible cyclooxygenase (COX-1 and COX-2) in the carbachol-induced ACTH and corticosterone secretion in non-stressed rats and animals exposed to social crowding stress for 7 days (24 per a cage for 6). Carbachol 71-80 cytochrome c oxidase I, mitochondrial Rattus norvegicus 275-280 12369741-5 2002 The present study sought to determine 1) the functional selectivity of carbachol for cholinergic muscarinic and/or nicotinic receptors involved in the stimulation of HPA axis; 2) the involvement of prostaglandins (PGs) generated by constitutive and inducible cyclooxygenase (COX-1 and COX-2) in the carbachol-induced ACTH and corticosterone secretion in non-stressed rats and animals exposed to social crowding stress for 7 days (24 per a cage for 6). Carbachol 71-80 cytochrome c oxidase II, mitochondrial Rattus norvegicus 285-290 12369741-21 2002 ), a COX-1 inhibitor, considerably impaired the carbachol-induced ACTH and corticosterone responses in control rats and markedly diminished these responses in stressed rats. Carbachol 48-57 cytochrome c oxidase I, mitochondrial Rattus norvegicus 5-10 12369741-24 2002 These results indicate that in the carbachol-induced HPA axis activation PGs generated by COX-1 are considerably and to a much greater extent involved than PGs generated by COX-2. Carbachol 35-44 cytochrome c oxidase I, mitochondrial Rattus norvegicus 90-95 12020766-8 2002 Pretreatment of the cells with PP2 markedly decreased Cch-induced ERK1/2 phosphorylation, suggesting a role of Src family of tyrosine kinases in the signal transduction pathway involved in ERK1/2 activation by mAChR. Carbachol 54-57 mitogen activated protein kinase 3 Rattus norvegicus 66-72 12020766-8 2002 Pretreatment of the cells with PP2 markedly decreased Cch-induced ERK1/2 phosphorylation, suggesting a role of Src family of tyrosine kinases in the signal transduction pathway involved in ERK1/2 activation by mAChR. Carbachol 54-57 mitogen activated protein kinase 3 Rattus norvegicus 189-195 12154174-0 2002 Carbachol triggers RyR-dependent Ca(2+) release via activation of IP(3) receptors in isolated rat gastric myocytes. Carbachol 0-9 ryanodine receptor 2 Rattus norvegicus 19-22 11994295-5 2002 In vivo, the ratio of bound GDP/GTP and phosphorylation of annexin 7 change in direct proportion to the extent of catecholamine release from chromaffin cells in response to stimulation by carbachol, or to inhibition by various protein kinase C inhibitors. Carbachol 188-197 annexin A7 Homo sapiens 59-68 12392895-3 2002 The response to the acetylcholine analogue, carbamylcholine, decreased from a 95+/-2% (+/-SEM, n=8) inhibition of beat rate in control cells to 18+/-2% (+/-SEM,n =8) in TGFbeta(1) treated cells. Carbachol 44-59 transforming growth factor beta 1 Gallus gallus 169-179 12215855-9 2002 F, P and VIP most effectively reversed the carbachol-induced tension of isolated human detrusor strips. Carbachol 43-52 vasoactive intestinal peptide Homo sapiens 9-12 12136125-9 2002 Adenovirus-mediated transfer of MGL cDNA into rat cortical neurons increased MGL expression and attenuated N-methyl-D-aspartate/carbachol-induced 2-AG accumulation in these cells. Carbachol 128-137 monoglyceride lipase Rattus norvegicus 32-35 12020766-1 2002 Carbachol (Cch), a muscarinic acetylcholine receptors (mAChR) agonist, produces time- and dose-dependent increases in mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) phosphorylation in nondifferentiated Fischer rat thyroid (FRT) epithelial cells. Carbachol 0-9 mitogen activated protein kinase 3 Rattus norvegicus 190-194 12020766-1 2002 Carbachol (Cch), a muscarinic acetylcholine receptors (mAChR) agonist, produces time- and dose-dependent increases in mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) phosphorylation in nondifferentiated Fischer rat thyroid (FRT) epithelial cells. Carbachol 0-9 Eph receptor B1 Rattus norvegicus 195-198 12020766-1 2002 Carbachol (Cch), a muscarinic acetylcholine receptors (mAChR) agonist, produces time- and dose-dependent increases in mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) phosphorylation in nondifferentiated Fischer rat thyroid (FRT) epithelial cells. Carbachol 11-14 mitogen activated protein kinase 3 Rattus norvegicus 190-194 12020766-1 2002 Carbachol (Cch), a muscarinic acetylcholine receptors (mAChR) agonist, produces time- and dose-dependent increases in mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) phosphorylation in nondifferentiated Fischer rat thyroid (FRT) epithelial cells. Carbachol 11-14 Eph receptor B1 Rattus norvegicus 195-198 12020766-2 2002 Cells pretreatment with the selective phospholipase C inhibitor U73122 resulted in a decrease of Cch-stimulated ERK1/2 phosphorylation. Carbachol 97-100 mitogen activated protein kinase 3 Rattus norvegicus 112-118 12020766-6 2002 Additionally, Cch-induced ERK1/2 phosphorylation was reduced after either inhibition of Ca(2+) influx or intracellular Ca(2+) release. Carbachol 14-17 mitogen activated protein kinase 3 Rattus norvegicus 26-32 12020766-7 2002 Nevertheless, Cch-mediated ERK1/2 activation was genistein sensitive, indicating the involvement of protein tyrosine kinases on the downstream signalling of mAChR. Carbachol 14-17 mitogen activated protein kinase 3 Rattus norvegicus 27-33 12011082-0 2002 Effects of glucose, exogenous insulin, and carbachol on C-peptide and insulin secretion from isolated perifused rat islets. Carbachol 43-52 insulin Homo sapiens 70-77 12011082-6 2002 Stimulation with carbachol plus 7 mm glucose enhanced both C-peptide and insulin secretion, and the further addition of 100 nm bovine insulin had no inhibitory effect on C-peptide secretory rates under this condition. Carbachol 17-26 insulin Bos taurus 73-80 12006602-5 2002 RGS3 and RGS5 ribozymes differentially enhanced carbachol- and angiotensin II-induced MAP kinase activity, respectively, whereas RGS2 and RGS7 ribozymes had no effect. Carbachol 48-57 regulator of G-protein signaling 3 Rattus norvegicus 0-4 12006602-5 2002 RGS3 and RGS5 ribozymes differentially enhanced carbachol- and angiotensin II-induced MAP kinase activity, respectively, whereas RGS2 and RGS7 ribozymes had no effect. Carbachol 48-57 regulator of G-protein signaling 5 Rattus norvegicus 9-13 12006602-9 2002 These results indicate the feasibility of using the ribozyme technology to determine the functional role of endogenous RGS proteins in signaling pathways and to define novel receptor-selective roles of endogenous RGS3 and RGS5 in modulating MAP kinase responses to either carbachol or angiotensin. Carbachol 272-281 regulator of G-protein signaling 3 Rattus norvegicus 213-217 12006602-9 2002 These results indicate the feasibility of using the ribozyme technology to determine the functional role of endogenous RGS proteins in signaling pathways and to define novel receptor-selective roles of endogenous RGS3 and RGS5 in modulating MAP kinase responses to either carbachol or angiotensin. Carbachol 272-281 regulator of G-protein signaling 5 Rattus norvegicus 222-226 12110618-6 2002 Inhibition of PKC-alpha by the indolocarbazole Go 6976 revealed that about 28% of carbachol-induced acid secretion was inhibited by PKC-alpha. Carbachol 82-91 protein kinase C alpha Homo sapiens 14-23 12110618-6 2002 Inhibition of PKC-alpha by the indolocarbazole Go 6976 revealed that about 28% of carbachol-induced acid secretion was inhibited by PKC-alpha. Carbachol 82-91 protein kinase C alpha Homo sapiens 132-141 12110618-7 2002 In the presence of Go 6976 approximately 64% of the carbachol-induced signal transduction is mediated by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII), and 14% is conveyed by PKC-epsilon as deduced from the inhibition with the bisindolylmaleimide Ro 31-8220. Carbachol 52-61 calcium/calmodulin dependent protein kinase II gamma Homo sapiens 105-150 12110618-7 2002 In the presence of Go 6976 approximately 64% of the carbachol-induced signal transduction is mediated by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII), and 14% is conveyed by PKC-epsilon as deduced from the inhibition with the bisindolylmaleimide Ro 31-8220. Carbachol 52-61 calcium/calmodulin dependent protein kinase II gamma Homo sapiens 152-158 12110618-9 2002 Inhibition of carbachol-induced acid secretion by TPA was accompanied by a decrease in CaMKII activity. Carbachol 14-23 calcium/calmodulin dependent protein kinase II gamma Homo sapiens 87-93 12091463-0 2002 Effect of ethanol on protein kinase Czeta and p70S6 kinase activation by carbachol: a possible mechanism for ethanol-induced inhibition of glial cell proliferation. Carbachol 73-82 ribosomal protein S6 kinase B1 Homo sapiens 46-58 12091463-2 2002 In this study we investigated the activation of p70S6 kinase (p70S6K) by carbachol in 1321 N1 astroctyoma cells. Carbachol 73-82 ribosomal protein S6 kinase B1 Homo sapiens 48-60 12091463-2 2002 In this study we investigated the activation of p70S6 kinase (p70S6K) by carbachol in 1321 N1 astroctyoma cells. Carbachol 73-82 ribosomal protein S6 kinase B1 Homo sapiens 62-68 12091463-3 2002 Carbachol induced a dose- and time-dependent activation of p70S6K, as evidenced by increased phosphorylation at Thr-389, Thr-421 and Ser-424, by increased p70S6K activity, and by a shift in its molecular weight. Carbachol 0-9 ribosomal protein S6 kinase B1 Homo sapiens 59-65 12091463-3 2002 Carbachol induced a dose- and time-dependent activation of p70S6K, as evidenced by increased phosphorylation at Thr-389, Thr-421 and Ser-424, by increased p70S6K activity, and by a shift in its molecular weight. Carbachol 0-9 ribosomal protein S6 kinase B1 Homo sapiens 155-161 12091463-5 2002 Carbachol-induced DNA synthesis was strongly inhibited by rapamycin, suggesting that p70S6K activation plays an important role in carbachol-induced cell proliferation. Carbachol 0-9 ribosomal protein S6 kinase B1 Homo sapiens 85-91 12091463-5 2002 Carbachol-induced DNA synthesis was strongly inhibited by rapamycin, suggesting that p70S6K activation plays an important role in carbachol-induced cell proliferation. Carbachol 130-139 ribosomal protein S6 kinase B1 Homo sapiens 85-91 12091463-7 2002 In the same range of concentrations, ethanol also inhibits carbachol-induced activation of PKCzeta and of p70S6K. Carbachol 59-68 protein kinase C zeta Homo sapiens 91-98 12091463-7 2002 In the same range of concentrations, ethanol also inhibits carbachol-induced activation of PKCzeta and of p70S6K. Carbachol 59-68 ribosomal protein S6 kinase B1 Homo sapiens 106-112 12124440-2 2002 In SK-N-SH neuroblastoma cells, treatment with the cholinergic agonist carbachol led to maximal induction of EGR1 1 h after stimulation. Carbachol 71-80 early growth response 1 Homo sapiens 109-113 12124440-3 2002 This was preceded by the phosphorylation of CREB, which peaked as early as 5 minutes after carbachol treatment. Carbachol 91-100 cAMP responsive element binding protein 1 Homo sapiens 44-48 12124440-4 2002 The levels of both EGR1 and phosphorylated CREB (pCREB) slowly decayed over 4-8 h. CREB phosphorylation and EGR1 induction showed similar sensitivity to carbachol concentration, with EC(50) values in the range of 1-10 microM, and the changes in both transcription factors were blocked by the muscarinic antagonist atropine. Carbachol 153-162 cAMP responsive element binding protein 1 Homo sapiens 43-47 12124440-4 2002 The levels of both EGR1 and phosphorylated CREB (pCREB) slowly decayed over 4-8 h. CREB phosphorylation and EGR1 induction showed similar sensitivity to carbachol concentration, with EC(50) values in the range of 1-10 microM, and the changes in both transcription factors were blocked by the muscarinic antagonist atropine. Carbachol 153-162 cAMP responsive element binding protein 1 Homo sapiens 50-54 12124440-4 2002 The levels of both EGR1 and phosphorylated CREB (pCREB) slowly decayed over 4-8 h. CREB phosphorylation and EGR1 induction showed similar sensitivity to carbachol concentration, with EC(50) values in the range of 1-10 microM, and the changes in both transcription factors were blocked by the muscarinic antagonist atropine. Carbachol 153-162 early growth response 1 Homo sapiens 108-112 12124440-6 2002 However, CREB phosphorylation by carbachol was largely unaffected by MAP kinase blockade. Carbachol 33-42 cAMP responsive element binding protein 1 Homo sapiens 9-13 12184734-5 2002 Next, we determined the effects of toluene on carbamylcholine-stimulated [35S]GTPgammaS binding using membrane fractions of CHO cell expressing hm2 receptors. Carbachol 46-61 cholinergic receptor muscarinic 2 Homo sapiens 144-147 12128250-3 2002 Carbachol treatment resulted in a rapid translocation of PKC-theta; to the membrane. Carbachol 0-9 protein kinase C, theta Mus musculus 57-66 12128250-6 2002 The regulation of PKC-alpha in response to carbachol was quite distinct from that produced by the PKC activator, PMA, which rapidly translocated PKC-alpha from the cytosol to the membrane without any increases in PKC-alpha in the cytosol. Carbachol 43-52 protein kinase C, alpha Mus musculus 18-27 12128250-6 2002 The regulation of PKC-alpha in response to carbachol was quite distinct from that produced by the PKC activator, PMA, which rapidly translocated PKC-alpha from the cytosol to the membrane without any increases in PKC-alpha in the cytosol. Carbachol 43-52 protein kinase C, alpha Mus musculus 18-21 12128250-7 2002 Confocal microscopy demonstrated an enhanced membrane localization of PKC-theta; and overall increased intensity of PKC-alpha staining in the cytosol accompanied by a characteristic membrane staining of PKC-alpha in the myotubes treated with carbachol. Carbachol 242-251 protein kinase C, theta Mus musculus 70-79 12128250-7 2002 Confocal microscopy demonstrated an enhanced membrane localization of PKC-theta; and overall increased intensity of PKC-alpha staining in the cytosol accompanied by a characteristic membrane staining of PKC-alpha in the myotubes treated with carbachol. Carbachol 242-251 protein kinase C, alpha Mus musculus 116-125 12128250-7 2002 Confocal microscopy demonstrated an enhanced membrane localization of PKC-theta; and overall increased intensity of PKC-alpha staining in the cytosol accompanied by a characteristic membrane staining of PKC-alpha in the myotubes treated with carbachol. Carbachol 242-251 protein kinase C, alpha Mus musculus 203-212 12016133-3 2002 Carbachol was the most potent inducer of COX-2 gene expression. Carbachol 0-9 mitochondrially encoded cytochrome c oxidase II Homo sapiens 41-46 12106807-4 2002 The PAR-2-activating peptide SLIGRL-NH(2), but not the inactive control peptide, when administered i.v., strongly suppressed gastric acid secretion in response to carbachol, pentagastrin or 2-deoxy-D-glucose in the rats with a pylorus ligation. Carbachol 163-172 F2R like trypsin receptor 1 Rattus norvegicus 4-9 12011984-5 2002 The nitric oxide synthase (NOS) inhibitor, NGmono-methyl-L-arginine (L-NMMA), blunted this effect only in LM3 cells while in LM2 cells the action of CARB was blocked by Nomega hydroxy-L-arginine (L-OH-Arg), which is known to inhibit the arginase pathway. Carbachol 149-153 nitric oxide synthase 1, neuronal Mus musculus 4-25 12016133-8 2002 Addition of SB-203580 with Ad.dom.neg.IkappaB almost completely blocked carbachol stimulation of COX-2 gene expression. Carbachol 72-81 mitochondrially encoded cytochrome c oxidase II Homo sapiens 97-102 12016133-12 2002 Selective COX-2 inhibitor NS-398 blocked carbachol-stimulated PGE(2) release without affecting basal PGE(2) production. Carbachol 41-50 mitochondrially encoded cytochrome c oxidase II Homo sapiens 10-15 12016133-14 2002 Carbachol induces COX-2 gene expression in the parietal cells through signaling pathways that involve intracellular Ca(2+), PKC, p38 kinase, and activation of NF-kappaB. Carbachol 0-9 mitochondrially encoded cytochrome c oxidase II Homo sapiens 18-23 12016133-14 2002 Carbachol induces COX-2 gene expression in the parietal cells through signaling pathways that involve intracellular Ca(2+), PKC, p38 kinase, and activation of NF-kappaB. Carbachol 0-9 nuclear factor kappa B subunit 1 Homo sapiens 159-168 11939793-5 2002 The basal and carbachol-stimulated shedding of APP and ACE from human SH-SY5Y neuroblastoma cells could not be differentiated by any of the hydroxamate compounds, implying that the same or very similar activities are involved in the constitutive and regulated shedding of these two proteins. Carbachol 14-23 angiotensin I converting enzyme Homo sapiens 55-58 11961129-7 2002 Activation by carbachol of endogenously expressed M(1) muscarinic receptors in human embryonic kidney 293 cells, induced the internalization of mGluR1 splice variants, which was partially blocked by pretreatment with inhibitors of either PKC or Ca(2+) calmodulin-dependent kinase II (CaMKII). Carbachol 14-23 glutamate metabotropic receptor 1 Homo sapiens 144-150 11961129-7 2002 Activation by carbachol of endogenously expressed M(1) muscarinic receptors in human embryonic kidney 293 cells, induced the internalization of mGluR1 splice variants, which was partially blocked by pretreatment with inhibitors of either PKC or Ca(2+) calmodulin-dependent kinase II (CaMKII). Carbachol 14-23 calcium/calmodulin dependent protein kinase II gamma Homo sapiens 284-290 11961129-10 2002 In addition, arrestin-2-GFP or arrestin-3-GFP underwent significant carbachol-induced translocation from cytosol to membrane in cells coexpressing mGluR1a or 1c but not in cells coexpressing mGluR1b. Carbachol 68-77 arrestin beta 1 Homo sapiens 13-23 11961129-10 2002 In addition, arrestin-2-GFP or arrestin-3-GFP underwent significant carbachol-induced translocation from cytosol to membrane in cells coexpressing mGluR1a or 1c but not in cells coexpressing mGluR1b. Carbachol 68-77 arrestin 3 Homo sapiens 31-41 12398158-3 2002 Among several spasmogens, neurokinin A was the most potent with the following order of potency: carbachol, prostaglandin F2alpha and acetylcholine. Carbachol 96-105 tachykinin precursor 1 Homo sapiens 26-38 11830588-0 2002 The role of endogenous human Trp4 in regulating carbachol-induced calcium oscillations in HEK-293 cells. Carbachol 48-57 transient receptor potential cation channel subfamily C member 4 Homo sapiens 29-33 11830588-4 2002 Expression of HTRP4 antisense inhibited 35% of the carbachol (CCh)-stimulated Ba(2+) entry and 46% of the OAG-stimulated Sr(2+) entry but in contrast had no effect on the thapsigargin-stimulated Ba(2+) or Sr(2+) entry. Carbachol 51-60 transient receptor potential cation channel subfamily C member 4 Homo sapiens 14-19 11830588-4 2002 Expression of HTRP4 antisense inhibited 35% of the carbachol (CCh)-stimulated Ba(2+) entry and 46% of the OAG-stimulated Sr(2+) entry but in contrast had no effect on the thapsigargin-stimulated Ba(2+) or Sr(2+) entry. Carbachol 62-65 transient receptor potential cation channel subfamily C member 4 Homo sapiens 14-19 11830588-6 2002 Of greater importance, HTRP4 antisense expression, but not HTRP3 antisense expression, blocked the sustained Ca(2+) oscillations produced by low doses of CCh (15 microm), arguing that receptor-stimulated rather than store-operated channels are involved in these sustained oscillations. Carbachol 154-157 transient receptor potential cation channel subfamily C member 4 Homo sapiens 23-28 11830588-8 2002 In summary, these data suggest that HTRP4 proteins in HEK-293 cells, differing from HTRP3 and HTRP1 proteins, do not serve as functional subunits of store-operated channels but do function as subunits for CCh- and OAG-stimulated channels. Carbachol 205-208 transient receptor potential cation channel subfamily C member 4 Homo sapiens 36-41 11965549-5 2002 Carbachol-mediated release of Ca(2+) from intracellular stores was significantly higher in Bax transfectants compared to control transfectants (empty vector). Carbachol 0-9 BCL2 associated X, apoptosis regulator Homo sapiens 91-94 12167246-5 2002 Whereas the cholinergic agonist, carbachol, impairs traffic from the trans-Golgi network to prelysosomes, causing Golgi, secretory, and lysosomal proteins to reflux into domains of the trans-Golgi network that communicate with the blm and to accumulate in the blm, EGF specifically causes a 2.6-fold (P < 0.05) increase in the beta-hexosaminidase content of the blm fraction, apparently by impairing traffic from early endosomes to prelysosome. Carbachol 33-42 O-GlcNAcase Homo sapiens 330-349 11830588-9 2002 Furthermore, evidence is provided for the first time for the involvement of a Trp isoform (HTRP4) in the formation of the channel responsible for both arachidonic acid-induced Ca(2+) entry and the Ca(2+) entry needed to sustain long term Ca(2+) oscillations induced by low doses of carbachol. Carbachol 282-291 transient receptor potential cation channel subfamily C member 4 Homo sapiens 91-96 11805119-8 2002 In comparison with untransfected cells depletion of intracellular calcium stores in hTRP7-expressing cells, using either carbachol or thapsigargin, produced a marked increase in the subsequent level of Ca(2+) influx. Carbachol 121-130 transient receptor potential cation channel subfamily C member 7 Homo sapiens 84-89 12148843-8 2002 We found that around two-thirds of cells tested still showed some swelling-induced Ca2+ release (SICR) even after maximal concentrations (10(-5) M - 10(-4) M) of carbachol had been applied to empty agonist-sensitive intracellular Ca2+ stores. Carbachol 162-171 carbonic anhydrase 2 Rattus norvegicus 230-233 11832335-8 2002 Stimulated fluid production by the glands from Cx32-deficient mice was abnormally low in female glands compared with controls at low topical doses of carbachol. Carbachol 150-159 gap junction protein, beta 1 Mus musculus 47-51 11982704-7 2002 RESULTS: Endothelin-3 induced a concentration-dependent increase in tone in both human and pos-sum strips (P < 0.05) and at 100 nmol/L represented 44.2 +/- 4.5% and 40.3 +/- 4.6% of carbachol-induced tone, respectively. Carbachol 185-194 endothelin 3 Homo sapiens 9-21 11714707-3 2002 Applying carbachol to the contralateral hippocampal slices from ischemic rats increased the phosphorylation of ERK1/2 but did not increase phosphorylation in the ipsilateral hippocampus. Carbachol 9-18 mitogen activated protein kinase 3 Rattus norvegicus 111-117 11714707-8 2002 Carbachol-stimulated tyrosine phosphorylation of the G alpha(q/11), inositol 1,4,5-trisphosphate formation, and association of G alpha(q/11) with phospholipase C beta 1 were attenuated in the ipsilateral hippocampus. Carbachol 0-9 phospholipase C beta 1 Rattus norvegicus 146-168 11804843-7 2002 Incubation of longitudinal muscle-myenteric plexus (LMMP) with IL-4 and IL-13 enhanced Carbachol-induced muscle contraction (R(max) 35.5 +/- 1.9 and 32.4 +/- 2.9%, respectively). Carbachol 87-96 interleukin 4 Mus musculus 63-67 11812654-8 2002 The sensitivity to guanosine 5"-[gamma-thio]trisphosphate (GTP-gamma-S) and carbachol stimulation of PLC-beta1 activity was increased by PA. Carbachol 76-85 phospholipase C beta 1 Homo sapiens 101-110 11889209-8 2002 Disruption of GJ communication of GCs is probably due to increased phosphorylation of connexin 43 at serine residues, as shown in immunoprecipitation experiments with carbachol-challenged GCs. Carbachol 167-176 gap junction protein alpha 1 Homo sapiens 86-97 11804843-7 2002 Incubation of longitudinal muscle-myenteric plexus (LMMP) with IL-4 and IL-13 enhanced Carbachol-induced muscle contraction (R(max) 35.5 +/- 1.9 and 32.4 +/- 2.9%, respectively). Carbachol 87-96 interleukin 13 Mus musculus 72-77 11834435-6 2002 RESULTS: Addition of 10 nM ghrelin to the perfusate significantly reduced the insulin response to the secretagogues glucose, arginine and carbachol, which act on the B-cell via different mechanisms, as well as the somatostatin response to arginine. Carbachol 138-147 ghrelin and obestatin prepropeptide Rattus norvegicus 27-34 11882609-8 2002 Stimulation of PKC by other means, such as phorbol-12-myristate-13-acetate or carbachol, also resulted in the redistribution of fluorescence in a manner similar to that observed for angiotensin II. Carbachol 78-87 protein kinase C beta Homo sapiens 15-18 11818389-14 2002 CONCLUSIONS: These results suggest that pretreatment of cultured rat retinal neurons with ACh or the nAChR agonists, nicotine and carbachol, has a protective action against glutamate neurotoxicity through nAChRs and that the dopamine release induced by nicotinic stimulation subsequently protects the retinal neurons by way of dopamine D1 receptors. Carbachol 130-139 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 101-106 11821019-4 2002 hP2X3 receptor desensitization was reversible and was not observed following the increase in intracellular Ca2+ levels produced by carbachol. Carbachol 131-140 purinergic receptor P2X 3 Homo sapiens 0-5 11790384-4 2002 In distal common bile duct, indomethacin (5.6 microM) unmasked potent contractile effects of endothelin-1 [EC(50) 7.8 (5.5-11.1) nM; E(MAX) 80+/-6% of response to 80 mM KCl] and enhanced the contractile potency of carbachol (585-fold at EC(50) level), but not cholecystokinin C-terminal octapeptide. Carbachol 214-223 endothelin-1 Cavia porcellus 93-105 11790384-3 2002 At 100 nM, endothelin-1 or sarafotoxin S6c (selective endothelin ET(B) receptor agonist) inhibited contractions of choledochal (but not papillary) sphincter of Oddi to carbachol (1 microM) by 63+/-5 and 45+/-9%, respectively. Carbachol 168-177 endothelin-1 Cavia porcellus 11-23 11673466-3 2002 The stimulation of the cells by carbachol initiated the translocation of green fluorescent protein-tagged wild type RhoA to the plasma membrane within a minute. Carbachol 32-41 ras homolog family member A Homo sapiens 116-120 11804848-4 2002 Carbamylcholine chloride (Carbachol, CCh), A-23187, and thapsigargin also inhibited eIF2B and protein synthesis, whereas bombesin and the CCK analog JMV-180 were without effect. Carbachol 0-24 eukaryotic translation initiation factor 2B subunit delta Rattus norvegicus 84-89 11804848-4 2002 Carbamylcholine chloride (Carbachol, CCh), A-23187, and thapsigargin also inhibited eIF2B and protein synthesis, whereas bombesin and the CCK analog JMV-180 were without effect. Carbachol 26-35 eukaryotic translation initiation factor 2B subunit delta Rattus norvegicus 84-89 11673466-4 2002 The change in MLC20 phosphorylation level after carbachol stimulation was monitored by using phospho-Ser-19-specific antibody recognizing the phosphorylated MLC20 in single cells. Carbachol 48-57 myosin light chain 12B Homo sapiens 14-19 12613913-0 2002 Prolactin inhibits carbachol-dependent secretion by lacrimal acinar cells in vitro. Carbachol 19-28 prolactin Homo sapiens 0-9 11673466-4 2002 The change in MLC20 phosphorylation level after carbachol stimulation was monitored by using phospho-Ser-19-specific antibody recognizing the phosphorylated MLC20 in single cells. Carbachol 48-57 myosin light chain 12B Homo sapiens 157-162 11673466-7 2002 Carbachol stimulation increased myosin phosphorylation within a minute both at the cortical and the central region. Carbachol 0-9 myosin heavy chain 14 Homo sapiens 32-38 12201629-3 2002 In the present study, we show that activation of synaptic inputs within and to the medial entorhinal cortex (mEC) of the in vitro isolated guinea pig brain preparation resets the phase of ongoing gamma activity induced by muscarinic receptor agonism with carbachol (frequency: 24 +/- 2 Hz at 32 degrees C). Carbachol 255-264 chemokine (C-C motif) ligand 28 Mus musculus 109-112 11780929-4 2002 Combined intracavernosal carbachol administration and submaximal CN electrical stimulation raised the recorded ICP, elicited by CN electrical stimulation alone in wild-type mice (from 35.7 +/- 2.7 to 48.1 +/- 5.5 mm Hg, P < .05) but not in eNOS-/ - mice (from 54.9 +/- 6.3 to 51.0 +/- 9.5 mm Hg, not significant [NS]). Carbachol 25-34 nitric oxide synthase 3, endothelial cell Mus musculus 243-247 11679428-6 2001 The effects of phorbol ester, CCh, and CCK were inhibited by as much as 75% by the PKC inhibitors GF 109203X and Ro-32-0432 and after PKC downregulation. Carbachol 30-33 protein kinase C, gamma Rattus norvegicus 83-86 14517621-10 2002 CCh-induced IP(1) accumulation was potentiated significantly by NE and Phe, but not by DA. Carbachol 0-3 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 12-17 14517621-11 2002 CONCLUSION: Although NE and Phe potentiated CCh-induced IP(1) accumulation, they could not potentiate CCh-induced contraction, suggesting that in clinical settings, vasopressors such as NE, DA, and Phe might be safely used in patients with asthma. Carbachol 44-47 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 56-61 11755214-3 2001 Carbachol-stimulated increases in endogenous RGS2 protein levels appeared by immunocytochemical analysis to be largely confined to the nucleus, and this localization was confirmed by Western blot analysis which showed increased nuclear, but not cytosolic, RGS2 after carbachol treatment. Carbachol 0-9 regulator of G protein signaling 2 Homo sapiens 256-260 11755214-3 2001 Carbachol-stimulated increases in endogenous RGS2 protein levels appeared by immunocytochemical analysis to be largely confined to the nucleus, and this localization was confirmed by Western blot analysis which showed increased nuclear, but not cytosolic, RGS2 after carbachol treatment. Carbachol 267-276 regulator of G protein signaling 2 Homo sapiens 45-49 11590149-5 2001 We show that following carbachol stimulation, m4 co-localizes with transferrin, and the selective marker of early endosomes, EEA1. Carbachol 23-32 transferrin Homo sapiens 67-78 11590149-5 2001 We show that following carbachol stimulation, m4 co-localizes with transferrin, and the selective marker of early endosomes, EEA1. Carbachol 23-32 early endosome antigen 1 Homo sapiens 125-129 11546796-6 2001 In addition, cleavage of ROCK-I was observed when cells overexpressing m1 muscarinic acetylcholine receptors were stimulated with carbachol. Carbachol 130-139 Rho associated coiled-coil containing protein kinase 1 Homo sapiens 25-31 11694521-3 2002 In support of this, carbachol increased PI 3-kinase activity in PI 3-kinase (p85) immunoprecipitates. Carbachol 20-29 phosphoinositide-3-kinase regulatory subunit 2 Homo sapiens 77-80 11927149-7 2002 Intracellular injection of the IP(3) receptor blocker heparin prevented both the mAchR-mediated occurrence of IP(3)-assisted CICR and enhancement of spike-frequency adaptation with 10 microM carbachol. Carbachol 191-200 inositol 1,4,5-trisphosphate receptor, type 3 Rattus norvegicus 31-45 11755214-2 2001 In human astrocytoma 1321N1 cells RGS2 expression was increased by activation of muscarinic receptors coupled to phosphoinositide signaling with carbachol, or by increased cyclic AMP production, demonstrating that both signaling systems can increase the expression of a RGS family member in a single cell type. Carbachol 145-154 regulator of G protein signaling 2 Homo sapiens 34-38 11755214-2 2001 In human astrocytoma 1321N1 cells RGS2 expression was increased by activation of muscarinic receptors coupled to phosphoinositide signaling with carbachol, or by increased cyclic AMP production, demonstrating that both signaling systems can increase the expression of a RGS family member in a single cell type. Carbachol 145-154 paired like homeodomain 2 Homo sapiens 34-37 11755214-3 2001 Carbachol-stimulated increases in endogenous RGS2 protein levels appeared by immunocytochemical analysis to be largely confined to the nucleus, and this localization was confirmed by Western blot analysis which showed increased nuclear, but not cytosolic, RGS2 after carbachol treatment. Carbachol 0-9 regulator of G protein signaling 2 Homo sapiens 45-49 11679428-6 2001 The effects of phorbol ester, CCh, and CCK were inhibited by as much as 75% by the PKC inhibitors GF 109203X and Ro-32-0432 and after PKC downregulation. Carbachol 30-33 protein kinase C, gamma Rattus norvegicus 134-137 12086906-6 2001 CCh induced time-and dose-dependent increases in the c-fos and c-jun early-response genes, which were blocked by m1 and m3 inhibition but not by m2 inhibition. Carbachol 0-3 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 53-58 11679428-9 2001 Stringent Ca(2+) depletion by removal of extracellular Ca(2+) and inclusion of BAPTA/AM allowed for increased cAMP production in response to CCh and CCK; PKC inhibitors and PKC downregulation prevented this stimulation. Carbachol 141-144 protein kinase C, gamma Rattus norvegicus 154-157 11606435-10 2001 Furthermore, gel shift assays indicated that carbachol also induced a time-dependent stimulation of the activator protein-1 DNA-binding activity, suggesting that activation of mAChRs may play a role in the modulation of gene expression in Sertoli cells. Carbachol 45-54 Jun proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 104-123 11922142-3 2001 The cholinergic agonist carbachol caused an atropine-sensitive ERK activation in the dendrites and somata CA1 pyramidal neurons. Carbachol 24-33 mitogen-activated protein kinase 1 Mus musculus 63-66 11922142-3 2001 The cholinergic agonist carbachol caused an atropine-sensitive ERK activation in the dendrites and somata CA1 pyramidal neurons. Carbachol 24-33 carbonic anhydrase 1 Mus musculus 106-109 11517225-2 2001 Transient and okadaic acid-sensitive inhibition by 70-85% of Ins(1,4,5)P(3) and Ins(1,3,4,5)P(4) 5-phosphatase activities was observed in homogenates from rat cortical astrocytes, human astrocytoma 1321N1 cells, and rat basophilic leukemia RBL-2H3 cells after incubation with carbachol. Carbachol 276-285 insulin 1 Rattus norvegicus 80-110 11557526-9 2001 However, Ht31, but not Ht31P, inhibited carbachol- and A23187-stimulated augmentation of secretion from cells preincubated with cholera toxin. Carbachol 40-49 A-kinase anchoring protein 13 Homo sapiens 9-13 11557586-3 2001 Forskolin-induced adenylyl cyclase activity was inhibited by carbachol in GGTI-2147-pretreated cells, demonstrating that the effect of geranylgeranyltransferase I inhibition on stress fiber formation was not due to uncoupling of signaling between the heterotrimeric G(i) protein (the Ggamma subunit is isoprenylated) and distal effectors. Carbachol 61-70 protein geranylgeranyltransferase type I subunit beta Homo sapiens 74-78 11846996-3 2001 AChR function measured by carbachol-induced (22)Na+ influx demonstrated that dimethoate may inhibit the nAChR function either by binding to a noncompetitive site and changing the conformational state of nAChR or by blocking the nAChR channel directly. Carbachol 26-35 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 104-109 11559781-11 2001 In PLB-SMOE bladders, in contrast, the response of [Ca2+]i and force to CCh was significantly increased and the EC50 values were decreased. Carbachol 72-75 phospholamban Mus musculus 3-6 11420052-9 2001 CCh also caused a dose-dependent increase in [Ca2+]i measured by fura-2 in microspectrofluorimetric studies, with a half-maximal response at approximately 3x10(-6) M. Neither isoproterenol (10(-5) M) nor substance P (10(-6) M) affected K+-channel activity or [Ca2+]i. Carbachol 0-3 tachykinin precursor 1 Homo sapiens 204-215 11564718-5 2001 Nutrients, such as palmitic acid, oleic acid, and meat hydrolysate, stimulated GLP-1 secretion in a dose-dependent manner, as did the cholinergic agonist carbachol and the neuromediator gastrin-releasing peptide. Carbachol 154-163 glucagon Homo sapiens 79-84 11533128-8 2001 The physiological concentration of secretin (50 pM) had a relatively weak effect on I(Ks) (160 % increase), which was significantly enhanced by transient co-stimulation with carbachol (CCh) (10 microM). Carbachol 174-183 secretin Rattus norvegicus 35-43 11533128-8 2001 The physiological concentration of secretin (50 pM) had a relatively weak effect on I(Ks) (160 % increase), which was significantly enhanced by transient co-stimulation with carbachol (CCh) (10 microM). Carbachol 185-188 secretin Rattus norvegicus 35-43 11443064-11 2001 Carbachol (100 microM) translocated only PKC epsilon and inhibited I(sc) with no effect on TER. Carbachol 0-9 protein kinase C epsilon Homo sapiens 41-52 11509335-0 2001 Interleukin-4 rapidly inhibits calcium transients in response to carbachol in bovine airway smooth muscle cells. Carbachol 65-74 interleukin 4 Bos taurus 0-13 11600318-4 2001 The effect of carbamylcholine was abolished by 10 microM BAPTA-AM (an intracellular Ca2+ chelator), 30 microM dantrolene (an inhibitor of ryanodinic receptors), 500 nM H-89 (an inhibitor of PKA), 1.25 microM chelerythrine chloride (an inhibitor of PKC) and 50 microM PD-98059 (a MEK inhibitor). Carbachol 14-29 mitogen-activated protein kinase kinase 7 Homo sapiens 279-282 11470473-4 2001 Dialyzed (30-100 microM) ODQ (1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one), a soluble guanylyl cyclase (sGC) inactivator, blocked inhibition of IBMX-stimulated I(Ca(L)) by SIN-1 (10 microM) but not by CCh (1-100 microM) or ANP (100 nM). Carbachol 201-204 sarcoglycan beta Homo sapiens 104-107 11470473-4 2001 Dialyzed (30-100 microM) ODQ (1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one), a soluble guanylyl cyclase (sGC) inactivator, blocked inhibition of IBMX-stimulated I(Ca(L)) by SIN-1 (10 microM) but not by CCh (1-100 microM) or ANP (100 nM). Carbachol 201-204 MAPK associated protein 1 Homo sapiens 172-177 11470473-6 2001 Thus CCh can increase cGMP synthesis via pGC. Carbachol 5-8 progastricsin Homo sapiens 41-44 11569612-3 2001 NOR 1, NO donor, relaxed the longitudinal muscle of the rat proximal colon, which was precontracted by carbachol, with a concomitant decrease in [Ca2+]. Carbachol 103-112 nuclear receptor subfamily 4 group A member 3 Homo sapiens 0-5 11460267-3 2001 Carbachol caused a rapid and transient phorphorylation of MAPK (ERK1/2) in all cell types, with an increase in MAPK activity, without changing the levels of MAPK proteins. Carbachol 0-9 mitogen-activated protein kinase 3 Homo sapiens 64-70 11460267-6 2001 Pretreatment of cells with the protein kinase C (PKC) inhibitor bisindolylmaleimide I, or downregulation of PKC by 24-h treatment with the phorbol ester TPA inhibited carbachol-induced MAPK activation. Carbachol 167-176 protein kinase C alpha Homo sapiens 49-52 11460267-6 2001 Pretreatment of cells with the protein kinase C (PKC) inhibitor bisindolylmaleimide I, or downregulation of PKC by 24-h treatment with the phorbol ester TPA inhibited carbachol-induced MAPK activation. Carbachol 167-176 protein kinase C alpha Homo sapiens 108-111 11460267-7 2001 Additional experiments with PKC alpha- or PKC epsilon-specific compounds indicated that the epsilon isozyme of PKC is primarily involved in MAPK activation by carbachol. Carbachol 159-168 protein kinase C alpha Homo sapiens 28-37 11460267-7 2001 Additional experiments with PKC alpha- or PKC epsilon-specific compounds indicated that the epsilon isozyme of PKC is primarily involved in MAPK activation by carbachol. Carbachol 159-168 protein kinase C epsilon Homo sapiens 42-53 11460267-7 2001 Additional experiments with PKC alpha- or PKC epsilon-specific compounds indicated that the epsilon isozyme of PKC is primarily involved in MAPK activation by carbachol. Carbachol 159-168 protein kinase C alpha Homo sapiens 28-31 11454952-4 2001 At 40 mM [BAPTA]pip, 100 microM CCh reversibly suppressed I(Ca(L)) maximally by 42%; half-maximal inhibition (20%) required 1 microM. Carbachol 32-35 prolactin-inducible protein homolog Cavia porcellus 16-19 11454952-7 2001 Inhibition by 100 microM CCh averaged -1.8 +/- 0.6, -2.3 +/- 0.4, and -4.1 +/- 0.4 pA/pF at 20, 30, and 40 mM [BAPTA](pip), respectively. Carbachol 25-28 prolactin-inducible protein homolog Cavia porcellus 118-121 11534853-4 2001 Activation of PLC through Galpha15 in response to carbachol did not increase cytosolic [Ca2+] ([Ca2+]i) but stimulated protein kinase C. While carbachol decreased the secretory activity in non-induced GH3 cells, it increased secretion in cells expressing Galpha15. Carbachol 50-59 G protein subunit alpha 15 Rattus norvegicus 26-34 11534853-4 2001 Activation of PLC through Galpha15 in response to carbachol did not increase cytosolic [Ca2+] ([Ca2+]i) but stimulated protein kinase C. While carbachol decreased the secretory activity in non-induced GH3 cells, it increased secretion in cells expressing Galpha15. Carbachol 143-152 G protein subunit alpha 15 Rattus norvegicus 26-34 11534853-4 2001 Activation of PLC through Galpha15 in response to carbachol did not increase cytosolic [Ca2+] ([Ca2+]i) but stimulated protein kinase C. While carbachol decreased the secretory activity in non-induced GH3 cells, it increased secretion in cells expressing Galpha15. Carbachol 143-152 G protein subunit alpha 15 Rattus norvegicus 255-263 11292831-2 2001 We first show that activation of ERK1/2 by the M(1) mAChR agonist carbachol takes place primarily via a Ras-independent pathway that depends largely upon Rap1, another small GTP-binding protein in the Ras family. Carbachol 66-75 mitogen activated protein kinase 3 Rattus norvegicus 33-39 11337491-6 2001 Muscarinic stimulation by carbachol reduced the alpha-synuclein oligomer in plasma membrane over a 30-min period, with a concomitant increase of both the oligomer and the monomer in the cytoplasmic fraction. Carbachol 26-35 synuclein alpha Homo sapiens 48-63 11337491-8 2001 The carbachol-induced alteration of alpha-synuclein was blocked by atropine. Carbachol 4-13 synuclein alpha Homo sapiens 36-51 11337491-9 2001 Translocation of the alpha-synuclein oligomer in response to carbachol stimulation corresponds closely with the time course of ligand-stimulated muscarinic receptor endocytosis. Carbachol 61-70 synuclein alpha Homo sapiens 21-36 11401853-5 2001 These data indicate that PI(4,5)P2 synthesis rates and other parameters of CCh-stimulated inositol phospholipid turnover are muscle length-dependent and provide evidence that supports the hypothesis that length-dependent beta1-integrin signals may exert control on CCh-activated PI(4,5)P2 synthesis. Carbachol 75-78 integrin subunit beta 1 Homo sapiens 221-235 11401853-5 2001 These data indicate that PI(4,5)P2 synthesis rates and other parameters of CCh-stimulated inositol phospholipid turnover are muscle length-dependent and provide evidence that supports the hypothesis that length-dependent beta1-integrin signals may exert control on CCh-activated PI(4,5)P2 synthesis. Carbachol 265-268 integrin subunit beta 1 Homo sapiens 221-235 11408264-5 2001 Basolateral, but not apical, addition of insulin inhibited carbachol- and thapsigargin-induced chloride secretion in a time- and concentration-dependent fashion. Carbachol 59-68 insulin Homo sapiens 41-48 11565612-2 2001 Treatment of differentiated cells with 1 mM carbachol caused rapid increases in the tyrosine phosphorylation of focal adhesion kinase (FAK), Cas, and paxillin. Carbachol 44-53 protein tyrosine kinase 2 Homo sapiens 112-133 11565612-2 2001 Treatment of differentiated cells with 1 mM carbachol caused rapid increases in the tyrosine phosphorylation of focal adhesion kinase (FAK), Cas, and paxillin. Carbachol 44-53 protein tyrosine kinase 2 Homo sapiens 135-138 11565612-3 2001 The src family kinase-selective inhibitor PP1 reduced carbachol-stimulated tyrosine phosphorylation of FAK, Cas, and paxillin by 50 to 75%. Carbachol 54-63 FYN proto-oncogene, Src family tyrosine kinase Homo sapiens 4-7 11565612-3 2001 The src family kinase-selective inhibitor PP1 reduced carbachol-stimulated tyrosine phosphorylation of FAK, Cas, and paxillin by 50 to 75%. Carbachol 54-63 neuropeptide Y receptor Y4 Homo sapiens 42-45 11565612-3 2001 The src family kinase-selective inhibitor PP1 reduced carbachol-stimulated tyrosine phosphorylation of FAK, Cas, and paxillin by 50 to 75%. Carbachol 54-63 protein tyrosine kinase 2 Homo sapiens 103-106 11565612-4 2001 In contrast, carbachol-stimulated activation of ERK1/2 was unaffected by PP1. Carbachol 13-22 mitogen-activated protein kinase 3 Homo sapiens 48-54 11565612-5 2001 Src family kinase activation by carbachol was further demonstrated by increased carbachol-induced tyrosine phosphorylation of the src-substrate, p120, and tyrosine phosphorylation of the src family kinase activation-associated autophosphorylation site. Carbachol 32-41 FYN proto-oncogene, Src family tyrosine kinase Homo sapiens 0-3 11565612-5 2001 Src family kinase activation by carbachol was further demonstrated by increased carbachol-induced tyrosine phosphorylation of the src-substrate, p120, and tyrosine phosphorylation of the src family kinase activation-associated autophosphorylation site. Carbachol 32-41 FYN proto-oncogene, Src family tyrosine kinase Homo sapiens 130-133 11565612-5 2001 Src family kinase activation by carbachol was further demonstrated by increased carbachol-induced tyrosine phosphorylation of the src-substrate, p120, and tyrosine phosphorylation of the src family kinase activation-associated autophosphorylation site. Carbachol 32-41 catenin delta 1 Homo sapiens 145-149 11565612-5 2001 Src family kinase activation by carbachol was further demonstrated by increased carbachol-induced tyrosine phosphorylation of the src-substrate, p120, and tyrosine phosphorylation of the src family kinase activation-associated autophosphorylation site. Carbachol 80-89 FYN proto-oncogene, Src family tyrosine kinase Homo sapiens 0-3 11565612-5 2001 Src family kinase activation by carbachol was further demonstrated by increased carbachol-induced tyrosine phosphorylation of the src-substrate, p120, and tyrosine phosphorylation of the src family kinase activation-associated autophosphorylation site. Carbachol 80-89 FYN proto-oncogene, Src family tyrosine kinase Homo sapiens 130-133 11565612-5 2001 Src family kinase activation by carbachol was further demonstrated by increased carbachol-induced tyrosine phosphorylation of the src-substrate, p120, and tyrosine phosphorylation of the src family kinase activation-associated autophosphorylation site. Carbachol 80-89 catenin delta 1 Homo sapiens 145-149 11565612-6 2001 Site-specific FAK phosphotyrosine antibodies were used to determine that the carbachol-stimulated increase in the autophosphorylation of FAK was unaffected by pretreatment with PP1, whereas the carbachol-stimulated increase in the src family kinase-mediated phosphotyrosine of FAK was completely blocked by pretreatment with PP1. Carbachol 77-86 protein tyrosine kinase 2 Homo sapiens 14-17 11565612-6 2001 Site-specific FAK phosphotyrosine antibodies were used to determine that the carbachol-stimulated increase in the autophosphorylation of FAK was unaffected by pretreatment with PP1, whereas the carbachol-stimulated increase in the src family kinase-mediated phosphotyrosine of FAK was completely blocked by pretreatment with PP1. Carbachol 77-86 protein tyrosine kinase 2 Homo sapiens 137-140 11565612-6 2001 Site-specific FAK phosphotyrosine antibodies were used to determine that the carbachol-stimulated increase in the autophosphorylation of FAK was unaffected by pretreatment with PP1, whereas the carbachol-stimulated increase in the src family kinase-mediated phosphotyrosine of FAK was completely blocked by pretreatment with PP1. Carbachol 77-86 FYN proto-oncogene, Src family tyrosine kinase Homo sapiens 231-234 11565612-6 2001 Site-specific FAK phosphotyrosine antibodies were used to determine that the carbachol-stimulated increase in the autophosphorylation of FAK was unaffected by pretreatment with PP1, whereas the carbachol-stimulated increase in the src family kinase-mediated phosphotyrosine of FAK was completely blocked by pretreatment with PP1. Carbachol 77-86 protein tyrosine kinase 2 Homo sapiens 137-140 11565612-6 2001 Site-specific FAK phosphotyrosine antibodies were used to determine that the carbachol-stimulated increase in the autophosphorylation of FAK was unaffected by pretreatment with PP1, whereas the carbachol-stimulated increase in the src family kinase-mediated phosphotyrosine of FAK was completely blocked by pretreatment with PP1. Carbachol 77-86 neuropeptide Y receptor Y4 Homo sapiens 325-328 11423385-4 2001 On the other hand, when carbachol and AVP were associated, a significant decrease of AVP hydrosmotic activity occurred (23%). Carbachol 24-33 arginine vasopressin Homo sapiens 85-88 11522294-2 2001 We report that apoE4, but not apoE3, disrupts carbachol-stimulated phosphoinositide (PI) hydrolysis in SH-SY5Y neuroblastoma cells. Carbachol 46-55 apolipoprotein E Homo sapiens 15-20 11522294-3 2001 Carbachol responses were also disrupted by beta-amyloid (Abeta) (1-42) and apoE4/Abeta(1-42) complexes, but not by apoE3/Abeta(1-42). Carbachol 0-9 apolipoprotein E Homo sapiens 75-91 11409731-2 2001 In this study, we report on the ability of carbachol to stimulate the phosphorylation of Akt/PKB, an important target of phosphatidylinositol 3 kinase (PI3 kinase) in 1321N1 human astrocytoma cells. Carbachol 43-52 AKT serine/threonine kinase 1 Homo sapiens 89-96 11409731-3 2001 Carbachol induced a dose-dependent phosphorylation of Ser473 on Akt, peaking after 15 min. Carbachol 0-9 AKT serine/threonine kinase 1 Homo sapiens 64-67 11375256-10 2001 In another set of rabbits, the same treatment with EPO also decreased vasodilating responses to carbachol, bradykinin and substance P besides ACh as compared with control group. Carbachol 96-105 erythropoietin Oryctolagus cuniculus 51-54 11375962-4 2001 RESULTS: Both cell-permeant serine protease inhibitors blocked amylase secretion in response to secretagogues that use calcium as a second messenger (e.g., cerulein, carbamylcholine, and bombesin) but not to those that use adenosine 3",5"-cyclic monophosphate (cAMP) as a second messenger (e.g., secretin and vasoactive intestinal polypeptide). Carbachol 166-181 coagulation factor II, thrombin Homo sapiens 28-43 11358895-0 2001 Inhibition of carbachol stimulated acid secretion by interleukin 1beta in rabbit parietal cells requires protein kinase C. BACKGROUND: Interleukin 1beta (IL-1beta) is a potent inhibitor of gastric acid secretion. Carbachol 14-23 interleukin-1 beta Oryctolagus cuniculus 53-70 11358895-0 2001 Inhibition of carbachol stimulated acid secretion by interleukin 1beta in rabbit parietal cells requires protein kinase C. BACKGROUND: Interleukin 1beta (IL-1beta) is a potent inhibitor of gastric acid secretion. Carbachol 14-23 interleukin-1 beta Oryctolagus cuniculus 135-152 11358895-0 2001 Inhibition of carbachol stimulated acid secretion by interleukin 1beta in rabbit parietal cells requires protein kinase C. BACKGROUND: Interleukin 1beta (IL-1beta) is a potent inhibitor of gastric acid secretion. Carbachol 14-23 interleukin-1 beta Oryctolagus cuniculus 154-162 11358895-6 2001 RESULTS: IL-1beta inhibited carbachol and A23187 stimulated acid secretion in a dose dependent manner. Carbachol 28-37 interleukin-1 beta Oryctolagus cuniculus 9-17 11358895-12 2001 CONCLUSIONS: IL-1beta directly inhibits parietal cell carbachol stimulated acid secretion. Carbachol 54-63 interleukin-1 beta Oryctolagus cuniculus 13-21 11292831-6 2001 Using specific antibodies, we show that a recently identified Rap1 GEF, calcium- and diacylglycerol-regulated guanine nucleotide exchange factor I (CalDAG-GEFI), is expressed in PC12D cells and that carbachol stimulates the formation of a complex containing CalDAG-GEFI, Rap1, and activated B-Raf. Carbachol 199-208 Rap guanine nucleotide exchange factor 5 Rattus norvegicus 67-70 11292831-6 2001 Using specific antibodies, we show that a recently identified Rap1 GEF, calcium- and diacylglycerol-regulated guanine nucleotide exchange factor I (CalDAG-GEFI), is expressed in PC12D cells and that carbachol stimulates the formation of a complex containing CalDAG-GEFI, Rap1, and activated B-Raf. Carbachol 199-208 RAS guanyl releasing protein 2 Rattus norvegicus 72-146 11292831-6 2001 Using specific antibodies, we show that a recently identified Rap1 GEF, calcium- and diacylglycerol-regulated guanine nucleotide exchange factor I (CalDAG-GEFI), is expressed in PC12D cells and that carbachol stimulates the formation of a complex containing CalDAG-GEFI, Rap1, and activated B-Raf. Carbachol 199-208 RAS guanyl releasing protein 2 Rattus norvegicus 148-159 11423385-5 2001 The inhibitory effect of carbachol on the AVP action was almost completely abolished by the cholinergic antagonists atropine, pirenzepine, 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP) and the calcium antagonist lanthanum. Carbachol 25-34 arginine vasopressin Homo sapiens 42-45 11292831-6 2001 Using specific antibodies, we show that a recently identified Rap1 GEF, calcium- and diacylglycerol-regulated guanine nucleotide exchange factor I (CalDAG-GEFI), is expressed in PC12D cells and that carbachol stimulates the formation of a complex containing CalDAG-GEFI, Rap1, and activated B-Raf. Carbachol 199-208 RAS guanyl releasing protein 2 Rattus norvegicus 258-269 11292831-6 2001 Using specific antibodies, we show that a recently identified Rap1 GEF, calcium- and diacylglycerol-regulated guanine nucleotide exchange factor I (CalDAG-GEFI), is expressed in PC12D cells and that carbachol stimulates the formation of a complex containing CalDAG-GEFI, Rap1, and activated B-Raf. Carbachol 199-208 B-Raf proto-oncogene, serine/threonine kinase Rattus norvegicus 291-296 11292831-7 2001 Finally, we show that expression of CalDAG-GEFI antisense RNA largely blocks carbachol-stimulated activation of hemagglutinin (HA)1-tagged B-Raf and formation of the CalDAG-GEFI/Rap1/HA1-tagged B-Raf complex. Carbachol 77-86 RAS guanyl releasing protein 2 Rattus norvegicus 36-47 11292831-7 2001 Finally, we show that expression of CalDAG-GEFI antisense RNA largely blocks carbachol-stimulated activation of hemagglutinin (HA)1-tagged B-Raf and formation of the CalDAG-GEFI/Rap1/HA1-tagged B-Raf complex. Carbachol 77-86 B-Raf proto-oncogene, serine/threonine kinase Rattus norvegicus 139-144 11292831-7 2001 Finally, we show that expression of CalDAG-GEFI antisense RNA largely blocks carbachol-stimulated activation of hemagglutinin (HA)1-tagged B-Raf and formation of the CalDAG-GEFI/Rap1/HA1-tagged B-Raf complex. Carbachol 77-86 RAS guanyl releasing protein 2 Rattus norvegicus 166-177 11292831-7 2001 Finally, we show that expression of CalDAG-GEFI antisense RNA largely blocks carbachol-stimulated activation of hemagglutinin (HA)1-tagged B-Raf and formation of the CalDAG-GEFI/Rap1/HA1-tagged B-Raf complex. Carbachol 77-86 B-Raf proto-oncogene, serine/threonine kinase Rattus norvegicus 194-199 11259416-4 2001 In HEK293 cells stably transfected with human TRPC3 channels, the actions of 2-APB to block carbachol-induced InsP(3)R-mediated store release and carbachol-induced Sr(2+) entry through TRPC3 channels were both reversed at high agonist levels, suggesting InsP(3)Rs mediate TRPC3 activation. Carbachol 92-101 transient receptor potential cation channel subfamily C member 3 Homo sapiens 46-51 11389190-9 2001 Functional consequences of these events are a reduction in carbachol-stimulated p42/44(MAPK) and CREB phosphorylation, as well as induction of c-fos. Carbachol 59-68 cyclin dependent kinase 20 Homo sapiens 80-83 11389190-9 2001 Functional consequences of these events are a reduction in carbachol-stimulated p42/44(MAPK) and CREB phosphorylation, as well as induction of c-fos. Carbachol 59-68 cAMP responsive element binding protein 1 Homo sapiens 97-101 11423971-5 2001 Although Gem expression is rare in epithelial and hematopoietic cancer cell lines, constitutive Gem levels were detected in several neuroblastoma cell lines and could be further induced as much as 10-fold following treatment with PMA or the acetylcholine muscarinic agonist, carbachol. Carbachol 275-284 GTP binding protein (gene overexpressed in skeletal muscle) Mus musculus 96-99 11292627-5 2001 As demonstrated previously for acute CO2-regulated cotransport activity, we found that inhibitors of Src family kinases (SFKs) or the classic mitogen-activated protein kinase (MAPK) pathway prevented the stimulation of NBC activity by carbachol. Carbachol 235-244 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 101-104 11292627-5 2001 As demonstrated previously for acute CO2-regulated cotransport activity, we found that inhibitors of Src family kinases (SFKs) or the classic mitogen-activated protein kinase (MAPK) pathway prevented the stimulation of NBC activity by carbachol. Carbachol 235-244 mitogen-activated protein kinase 3 Homo sapiens 176-180 11292627-6 2001 The ability of carbachol to activate Src, as well as the proximal (Raf) and distal [extracellular signal-regulated kinases 1 and 2 (ERK1/2)] elements of the classic MAPK module, was compatible with these findings. Carbachol 15-24 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 37-40 11292627-6 2001 The ability of carbachol to activate Src, as well as the proximal (Raf) and distal [extracellular signal-regulated kinases 1 and 2 (ERK1/2)] elements of the classic MAPK module, was compatible with these findings. Carbachol 15-24 mitogen-activated protein kinase 3 Homo sapiens 165-169 11323367-4 2001 Carbachol (0.5-4.0 microg) administered into the mPRF produced significant dose- and time-dependent antinociception, sedation, and motor dysfunction. Carbachol 0-9 Spi-C transcription factor (Spi-1/PU.1 related) Mus musculus 49-53 11323367-7 2001 These results suggest that the antinociceptive action of carbachol is mediated by muscarinic cholinergic receptor activation, especially by M(2) receptor subtype in mPRF and spinal cord, and that although oxycodone seems unlikely to affect the cholinergic transmission of mPRF, spinal oxycodone-induced analgesia is at least partly mediated via the activation of M(2) receptor subtype at the spinal cord. Carbachol 57-66 Spi-C transcription factor (Spi-1/PU.1 related) Mus musculus 165-169 11292391-14 2001 The anti-adrenergic effect of ADO on I(NaCa) was mimicked by externally applied carbachol (CCh, 10 microM), a muscarinic receptor agonist. Carbachol 80-89 nascent polypeptide-associated complex subunit alpha Cavia porcellus 39-43 11292391-14 2001 The anti-adrenergic effect of ADO on I(NaCa) was mimicked by externally applied carbachol (CCh, 10 microM), a muscarinic receptor agonist. Carbachol 91-94 nascent polypeptide-associated complex subunit alpha Cavia porcellus 39-43 11316737-1 2001 Inositol 1,4,5-trisphosphate receptor (IP3R) protein levels in isolated rat pancreatic islets were investigated in response to carbachol (CCh) and sulfated cholecystokinin 26-33 amide stimulation. Carbachol 127-136 inositol 1,4,5-trisphosphate receptor, type 1 Rattus norvegicus 39-43 11316737-1 2001 Inositol 1,4,5-trisphosphate receptor (IP3R) protein levels in isolated rat pancreatic islets were investigated in response to carbachol (CCh) and sulfated cholecystokinin 26-33 amide stimulation. Carbachol 138-141 inositol 1,4,5-trisphosphate receptor, type 1 Rattus norvegicus 39-43 11316737-2 2001 Within 2 h, CCh reduced IP3R-I protein levels by 22% and IP3R-II and -III levels to 65% or more below basal. Carbachol 12-15 inositol 1,4,5-trisphosphate receptor, type 1 Rattus norvegicus 24-28 11316737-2 2001 Within 2 h, CCh reduced IP3R-I protein levels by 22% and IP3R-II and -III levels to 65% or more below basal. Carbachol 12-15 inositol 1,4,5-trisphosphate receptor, type 1 Rattus norvegicus 57-61 11316737-4 2001 The effect of CCh was concentration- and time-dependent, with a persistent decline in IP3R levels for up to 6 h after the onset of stimulation. Carbachol 14-17 inositol 1,4,5-trisphosphate receptor, type 1 Rattus norvegicus 86-90 11316737-6 2001 Proteasome inhibition completely blocked the down-regulatory effects of CCh on IP3Rs and significantly increased the insulin secretory response to glucose stimulation in the presence of CCH: Islet stimulation by glucose, alpha-ketoisocaproic acid, and tolbutamide completely protected IP3Rs against the down-regulatory effects of CCH: 2-deoxyglucose and 3-O-methyl glucose failed to affect CCh-induced IP3R down-regulation. Carbachol 72-75 inositol 1,4,5-trisphosphate receptor, type 1 Rattus norvegicus 79-83 11311050-5 2001 We found that: (a) PGF(2alpha)and CCh increased p38 MAP kinase activity by 197 and 215%, respectively, and increased p42/p44 MAP kinase activity by 200 and 125%, respectively. Carbachol 34-37 mitogen-activated protein kinase 14 Homo sapiens 48-51 11311050-5 2001 We found that: (a) PGF(2alpha)and CCh increased p38 MAP kinase activity by 197 and 215%, respectively, and increased p42/p44 MAP kinase activity by 200 and 125%, respectively. Carbachol 34-37 cyclin dependent kinase 20 Homo sapiens 117-120 11311050-5 2001 We found that: (a) PGF(2alpha)and CCh increased p38 MAP kinase activity by 197 and 215%, respectively, and increased p42/p44 MAP kinase activity by 200 and 125%, respectively. Carbachol 34-37 interferon induced protein 44 Homo sapiens 121-124 11311050-6 2001 (b) SB202190, a p38 MAP kinase specific inhibitor, inhibited PGF(2alpha)- and CCh-induced cPLA(2)phosphorylation by 92 and 85%, respectively, and AA release by 62 and 78%, respectively. Carbachol 78-81 mitogen-activated protein kinase 14 Homo sapiens 16-19 11311050-7 2001 (c) PD98059, a p42/p44 MAP kinase inhibitor, inhibited CCh-induced cPLA(2)phosphorylation by 70% and AA release by 71%, but had no effect on that of PGF(2alpha). Carbachol 55-58 cyclin dependent kinase 20 Homo sapiens 15-18 11311050-7 2001 (c) PD98059, a p42/p44 MAP kinase inhibitor, inhibited CCh-induced cPLA(2)phosphorylation by 70% and AA release by 71%, but had no effect on that of PGF(2alpha). Carbachol 55-58 interferon induced protein 44 Homo sapiens 19-22 11311050-8 2001 (d) Inhibition of PKC activity by RO 31-8220 inhibited both PGF(2alpha)- and CCh-stimulation of p38 MAP kinase, p42/p44 MAP kinases and cPLA(2)phosphorylation. Carbachol 77-80 mitogen-activated protein kinase 14 Homo sapiens 96-99 11311050-8 2001 (d) Inhibition of PKC activity by RO 31-8220 inhibited both PGF(2alpha)- and CCh-stimulation of p38 MAP kinase, p42/p44 MAP kinases and cPLA(2)phosphorylation. Carbachol 77-80 cyclin dependent kinase 20 Homo sapiens 112-115 11311050-8 2001 (d) Inhibition of PKC activity by RO 31-8220 inhibited both PGF(2alpha)- and CCh-stimulation of p38 MAP kinase, p42/p44 MAP kinases and cPLA(2)phosphorylation. Carbachol 77-80 interferon induced protein 44 Homo sapiens 116-119 11259416-4 2001 In HEK293 cells stably transfected with human TRPC3 channels, the actions of 2-APB to block carbachol-induced InsP(3)R-mediated store release and carbachol-induced Sr(2+) entry through TRPC3 channels were both reversed at high agonist levels, suggesting InsP(3)Rs mediate TRPC3 activation. Carbachol 92-101 transient receptor potential cation channel subfamily C member 3 Homo sapiens 185-190 11259416-4 2001 In HEK293 cells stably transfected with human TRPC3 channels, the actions of 2-APB to block carbachol-induced InsP(3)R-mediated store release and carbachol-induced Sr(2+) entry through TRPC3 channels were both reversed at high agonist levels, suggesting InsP(3)Rs mediate TRPC3 activation. Carbachol 92-101 transient receptor potential cation channel subfamily C member 3 Homo sapiens 185-190 11287327-3 2001 Acute exposure to angiostatin or endostatin nearly abolished subsequent endothelial [Ca(2+)](i) responses to carbachol or to thapsigargin; conversely, thapsigargin attenuated the Ca(2+) signal elicited by endostatin. Carbachol 109-118 plasminogen Mus musculus 18-29 11287327-3 2001 Acute exposure to angiostatin or endostatin nearly abolished subsequent endothelial [Ca(2+)](i) responses to carbachol or to thapsigargin; conversely, thapsigargin attenuated the Ca(2+) signal elicited by endostatin. Carbachol 109-118 collagen, type XVIII, alpha 1 Mus musculus 33-43 11303068-4 2001 Thapsigargin, xestospongin C, and carbachol/Ca(2+)-free buffer blocked significantly the PCB-induced Ca(2+) transient, whereas both ryanodine (to deplete ryanodine-sensitive stores) and the L-type Ca(2+) channel blocker nifedipine were without effect on the A1254 initial Ca(2+) transient. Carbachol 34-43 pyruvate carboxylase Rattus norvegicus 89-92 11306714-6 2001 Scabronine G-ME concentration-dependently inhibited the carbachol-induced inositol phosphate accumulation in 1321N1 cells, which was reversed by GF109203X, an inhibitor of protein kinase C (PKC) isoforms. Carbachol 56-65 protein kinase C zeta Homo sapiens 190-193 11322774-4 2001 RGS2 mRNA levels were increased in differentiated cells by heat shock, carbachol, and activation of protein kinase C. After transient transfection of GFP-tagged RGS2, a predominant nuclear localization was observed by confocal microscopy. Carbachol 71-80 regulator of G protein signaling 2 Homo sapiens 0-4 11322774-4 2001 RGS2 mRNA levels were increased in differentiated cells by heat shock, carbachol, and activation of protein kinase C. After transient transfection of GFP-tagged RGS2, a predominant nuclear localization was observed by confocal microscopy. Carbachol 71-80 regulator of G protein signaling 2 Homo sapiens 161-165 11379045-7 2001 In addition, both IFN gamma and carbachol increase prostaglandin E2 production in SMG, but while indomethacin potentiates IFN gamma effect on amylase secretion, it blunted amylase secretion exerted by carbachol. Carbachol 201-210 interferon gamma Mus musculus 18-27 11379045-8 2001 Thus, IFN gamma and carbachol stimulate IFN gamma secretion on SMG in a dose-dependent manner. Carbachol 20-29 interferon gamma Mus musculus 40-49 11259416-4 2001 In HEK293 cells stably transfected with human TRPC3 channels, the actions of 2-APB to block carbachol-induced InsP(3)R-mediated store release and carbachol-induced Sr(2+) entry through TRPC3 channels were both reversed at high agonist levels, suggesting InsP(3)Rs mediate TRPC3 activation. Carbachol 146-155 transient receptor potential cation channel subfamily C member 3 Homo sapiens 46-51 11352632-0 2001 Carbachol stimulates TYR phosphorylation and association of PKCdelta and PYK2 in pancreas. Carbachol 0-9 protein tyrosine kinase 2 beta Rattus norvegicus 73-77 11259416-4 2001 In HEK293 cells stably transfected with human TRPC3 channels, the actions of 2-APB to block carbachol-induced InsP(3)R-mediated store release and carbachol-induced Sr(2+) entry through TRPC3 channels were both reversed at high agonist levels, suggesting InsP(3)Rs mediate TRPC3 activation. Carbachol 146-155 transient receptor potential cation channel subfamily C member 3 Homo sapiens 185-190 11352632-2 2001 The Ca2+-dependent tyrosine kinase PYK2 coimmunoprecipitated with PKCdelta from carbachol-exposed cells and also exhibited increased tyrosine phosphorylation. Carbachol 80-89 protein tyrosine kinase 2 beta Rattus norvegicus 35-39 11352632-3 2001 Tyrosine phosphorylation of both PKCdelta and PYK2 was concentration-dependent with respect to carbachol, and rapid, reaching maximal levels by 5 min of treatment. Carbachol 95-104 protein tyrosine kinase 2 beta Rattus norvegicus 46-50 11259416-4 2001 In HEK293 cells stably transfected with human TRPC3 channels, the actions of 2-APB to block carbachol-induced InsP(3)R-mediated store release and carbachol-induced Sr(2+) entry through TRPC3 channels were both reversed at high agonist levels, suggesting InsP(3)Rs mediate TRPC3 activation. Carbachol 146-155 transient receptor potential cation channel subfamily C member 3 Homo sapiens 185-190 11245606-4 2001 Carbachol enhances photoaffinity labeling ([alpha-(32)P]GTP-azidoaniline) of only 42-kDa proteins that are subsequently tractable to immunoprecipitation by antibodies specific for Galpha(q) or Galpha(11) but not Galpha(12) or Galpha(13). Carbachol 0-9 G protein subunit alpha q Rattus norvegicus 180-203 11245606-4 2001 Carbachol enhances photoaffinity labeling ([alpha-(32)P]GTP-azidoaniline) of only 42-kDa proteins that are subsequently tractable to immunoprecipitation by antibodies specific for Galpha(q) or Galpha(11) but not Galpha(12) or Galpha(13). Carbachol 0-9 G protein subunit alpha 12 Rattus norvegicus 212-222 11245606-4 2001 Carbachol enhances photoaffinity labeling ([alpha-(32)P]GTP-azidoaniline) of only 42-kDa proteins that are subsequently tractable to immunoprecipitation by antibodies specific for Galpha(q) or Galpha(11) but not Galpha(12) or Galpha(13). Carbachol 0-9 G protein subunit alpha 13 Rattus norvegicus 226-236 11347653-4 2001 By contrast, the CCh-stimulated initial [Ca2+]i increase was reduced at 0.5 and 4 h post-irradiation in all cell types and remained decreased at 24 h in wild-type and control-transfected cells, but recovered in Bcl-2- and Bcl-XL-transfectants. Carbachol 17-20 BCL2 apoptosis regulator Homo sapiens 211-216 11347653-4 2001 By contrast, the CCh-stimulated initial [Ca2+]i increase was reduced at 0.5 and 4 h post-irradiation in all cell types and remained decreased at 24 h in wild-type and control-transfected cells, but recovered in Bcl-2- and Bcl-XL-transfectants. Carbachol 17-20 BCL2 like 1 Homo sapiens 222-228 11347653-5 2001 The formation of inositol 1,4,5-trisphosphate (IP3) in response to CCh at 4-h post-irradiation was decreased in wild-type and control-transfected cells, but not in Bcl-2 and Bcl-XL transfectants. Carbachol 67-70 BCL2 like 1 Homo sapiens 174-180 11383923-2 2001 In pancreases from fed rats, leptin failed to alter the insulin secretion elicited by glucose, arginine or tolbutamide, but inhibited the insulin response to both CCK-8 and carbachol, secretagogues known to act on the B-cell by increasing phospholipid turnover. Carbachol 173-182 leptin Rattus norvegicus 29-35 11353015-18 2001 In conclusion, st. radiatum interneurons of CA3 hippocampal region represent a class of nonpyramidal cells with action potentials followed by an AHP of relatively short duration, partially generated by apamin and carbachol-sensitive conductances involved in the regulation of the cell firing rate. Carbachol 213-222 carbonic anhydrase 3 Rattus norvegicus 44-47 11383923-7 2001 Leptin inhibition of carbachol-induced insulin output might reflect a restraining effect of this peptide on the cholinergic stimulation of insulin release. Carbachol 21-30 leptin Rattus norvegicus 0-6 11259766-0 2001 Intracerebroventricular carbachol induces FOS immunoreactivity in lamina terminalis neurons projecting to the supraoptic nucleus. Carbachol 24-33 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 42-45 11679020-10 2001 Further evidence for muscarininc receptor antagonism by tacrine was a small rightward shift of the concentration-response curve for carbachol, an agonist immune to cholinesterase. Carbachol 132-141 butyrylcholinesterase Rattus norvegicus 164-178 11305656-3 2001 The nicotinic acetylcholine receptor (nAChr) agonists acetylcholine, carbachol, and (-)-nicotine were fractionated and detected by patch-clamped pheochromcytoma detector cells. Carbachol 69-78 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 38-43 11181542-2 2001 In dog thyroid primary cultures, the use of the phosphodiesterase-resistant analog of cAMP (Bu)(2)cAMP instead of TSH allowed to unveil a potent comitogenic activity of carbamylcholine, which can substitute for insulin and was shown to mimic insulin action on cell cycle regulatory proteins. Carbachol 169-184 insulin Canis lupus familiaris 211-218 11181542-2 2001 In dog thyroid primary cultures, the use of the phosphodiesterase-resistant analog of cAMP (Bu)(2)cAMP instead of TSH allowed to unveil a potent comitogenic activity of carbamylcholine, which can substitute for insulin and was shown to mimic insulin action on cell cycle regulatory proteins. Carbachol 169-184 insulin Canis lupus familiaris 242-249 11181542-3 2001 Like insulin, carbamylcholine induced the accumulation of cyclin D3 and overcame the repression by cAMP of this protein, which was shown 1) to be essential for cell cycle progression by means of microinjections of a neutralizing antibody; and 2) to be rate limiting for the cAMP-dependent assembly of cyclin D3-cdk4 complexes, their nuclear translocation and the phosphorylation of pRb. Carbachol 14-29 insulin Canis lupus familiaris 5-12 11181542-3 2001 Like insulin, carbamylcholine induced the accumulation of cyclin D3 and overcame the repression by cAMP of this protein, which was shown 1) to be essential for cell cycle progression by means of microinjections of a neutralizing antibody; and 2) to be rate limiting for the cAMP-dependent assembly of cyclin D3-cdk4 complexes, their nuclear translocation and the phosphorylation of pRb. Carbachol 14-29 cyclin D3 Canis lupus familiaris 58-67 11181542-3 2001 Like insulin, carbamylcholine induced the accumulation of cyclin D3 and overcame the repression by cAMP of this protein, which was shown 1) to be essential for cell cycle progression by means of microinjections of a neutralizing antibody; and 2) to be rate limiting for the cAMP-dependent assembly of cyclin D3-cdk4 complexes, their nuclear translocation and the phosphorylation of pRb. Carbachol 14-29 cyclin D3 Canis lupus familiaris 301-315 11245595-0 2001 PLD pathway involved in carbachol-induced Cl- secretion: possible role of TNF-alpha. Carbachol 24-33 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 0-3 11245595-0 2001 PLD pathway involved in carbachol-induced Cl- secretion: possible role of TNF-alpha. Carbachol 24-33 tumor necrosis factor Homo sapiens 74-83 11245595-1 2001 In a previous study, it was found that exposure to tumor necrosis factor-alpha (TNF-alpha) potentiated the electrophysiological response to carbachol in a time-dependent and cycloheximide-sensitive manner. Carbachol 140-149 tumor necrosis factor Homo sapiens 51-78 11245595-1 2001 In a previous study, it was found that exposure to tumor necrosis factor-alpha (TNF-alpha) potentiated the electrophysiological response to carbachol in a time-dependent and cycloheximide-sensitive manner. Carbachol 140-149 tumor necrosis factor Homo sapiens 80-89 11245595-5 2001 The aim of the present study was to investigate whether the phospholipase D (PLD) pathway plays a role in the carbachol response and the potentiating effect of TNF-alpha. Carbachol 110-119 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 60-75 11245595-5 2001 The aim of the present study was to investigate whether the phospholipase D (PLD) pathway plays a role in the carbachol response and the potentiating effect of TNF-alpha. Carbachol 110-119 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 77-80 11259487-2 2001 However, when DPDPE was applied during concomitant Gq-coupled m3 muscarinic receptor stimulation by carbachol or oxotremorine-M, it produced an elevation of [Ca(2+)](i). Carbachol 100-109 cholinergic receptor muscarinic 3 Homo sapiens 62-84 11166329-6 2001 When challenged with carbachol, IRK1 currents recorded from cells co-transfected with Galpha(q) were potently inhibited compared with controls. Carbachol 21-30 potassium inwardly rectifying channel subfamily J member 2 Homo sapiens 32-36 11222647-4 2001 We demonstrate that stimulation of these mAChRs with carbachol, a muscarinic agonist, activated extracellular-regulated kinases (Erk1/2) and phosphatidylinositol-3 kinase (PI-3K). Carbachol 53-62 mitogen activated protein kinase 3 Rattus norvegicus 129-135 11166329-6 2001 When challenged with carbachol, IRK1 currents recorded from cells co-transfected with Galpha(q) were potently inhibited compared with controls. Carbachol 21-30 succinate-CoA ligase GDP/ADP-forming subunit alpha Homo sapiens 86-92 11166329-8 2001 Concentration response curves revealed that carbachol was 16 times more potent at inhibiting IRK1 currents in cells co-transfected with Galpha(q) as compared with Galpha(12) co-transfected cells. Carbachol 44-53 potassium inwardly rectifying channel subfamily J member 2 Homo sapiens 93-97 11166329-8 2001 Concentration response curves revealed that carbachol was 16 times more potent at inhibiting IRK1 currents in cells co-transfected with Galpha(q) as compared with Galpha(12) co-transfected cells. Carbachol 44-53 succinate-CoA ligase GDP/ADP-forming subunit alpha Homo sapiens 136-142 11172778-0 2001 GABAergic neurons of the laterodorsal and pedunculopontine tegmental nuclei of the cat express c-fos during carbachol-induced active sleep. Carbachol 108-117 Fos proto-oncogene, AP-1 transcription factor subunit Felis catus 95-100 11287096-7 2001 Leptin and carbachol also caused an increased intracellular content of NPY. Carbachol 11-20 neuropeptide Y Homo sapiens 71-74 11172778-3 2001 Consequently, we sought to determine if these neurons are activated (as indicated by their c-fos expression) during active sleep induced by the microinjection of carbachol into the rostro-dorsal pons (AS-carbachol). Carbachol 162-171 Fos proto-oncogene, AP-1 transcription factor subunit Felis catus 91-96 11172737-5 2001 Culture of islets or betaTC3 cells with carbachol (0.5 mM) reduced IP3R-I mRNA expression levels below control. Carbachol 40-49 inositol 1,4,5-trisphosphate receptor 1 Mus musculus 67-71 11256182-1 2001 Since the early "60s, injections of a broad-spectrum muscarinic cholinergic agonist, carbachol, into the medial pontine reticular formation (mPRF) of cats have been extensively used as a tool with which to study the neural mechanisms of rapid eye movement (REM) sleep. Carbachol 85-94 Spi-C transcription factor (Spi-1/PU.1 related) Mus musculus 141-145 11256182-9 2001 While carbachol and many other neurotransmitters and peptides microinjected into the mPRF evoke, enhance or suppress REM sleep, the most sensitive site(s) of their actions have not been fully mapped, and the nature of the cellular and neurochemical interactions taking place at the sites where carbachol triggers the REM sleep-like state remain largely unknown. Carbachol 6-15 Spi-C transcription factor (Spi-1/PU.1 related) Mus musculus 85-89 11256182-10 2001 Similarly, little is known about the pathways between the mPRF and medial medullary reticular formation, but the existing evidence suggests that they are reciprocal and essential for the generation of both natural and carbachol-induced REM sleep. Carbachol 218-227 Spi-C transcription factor (Spi-1/PU.1 related) Mus musculus 58-62 11172737-7 2001 Culture of islets for up to 6 hr with carbachol reduced IP3R-I and -III protein expression in a time-dependent manner with a half-maximal effect on type I at 1 hr. Carbachol 38-47 inositol 1,4,5-trisphosphate receptor 1 Mus musculus 56-60 11172737-9 2001 The carbachol-induced decrease in IP3R-I and -III protein expression was reversed by carbobenzoxyl-leucinyl-leucinyl-leucinyl-H (MG-132), a proteasome inhibitor. Carbachol 4-13 inositol 1,4,5-trisphosphate receptor 1 Mus musculus 34-38 11172737-10 2001 Thus, glucose failed to regulate mouse islet IP3R mRNA expression, whereas carbachol stimulation down-regulated IP3R mRNA and protein. Carbachol 75-84 inositol 1,4,5-trisphosphate receptor 1 Mus musculus 112-116 11165010-4 2001 This is likely due to the effect of carbachol, observed after 24 h, to increase the levels of steroid acute regulatory (StAR) protein, as found in Western blots. Carbachol 36-45 steroidogenic acute regulatory protein Homo sapiens 120-124 11181421-12 2001 Carbachol and Tg produced further increases in calcium/GTP-induced tone and, in both cases, this additional tone was relaxed by NO and 8-Br-cyclic GMP. Carbachol 0-9 5'-nucleotidase, cytosolic II Mus musculus 147-150 11769308-3 2001 In a network model of area CA3, the time scale for CCH-delta corresponded to the decay constant of the gating variable of the calcium-dependent potassium (K-AHP) current, that of CCH-theta to an intrinsic subthreshold membrane potential oscillation of the pyramidal cells, and that of CCH-gamma to the decay constant of GABAergic inhibitory synaptic potentials onto the pyramidal cells. Carbachol 51-54 carbonic anhydrase 3 Rattus norvegicus 27-30 11159694-5 2001 A concentration-dependent increase in cyclic GMP production was observed in HEL299 cells incubated with carbachol, cocaine, or MEG for 24 h. The increase in cyclic GMP content was 3.6 fold for 1 microM carbachol (P < 0.01), 3.1 fold for 1 microM cocaine (P < 0.01), and 7.8 fold for 1 microM MEG (P < 0.001), respectively. Carbachol 104-113 5'-nucleotidase, cytosolic II Homo sapiens 45-48 11159694-5 2001 A concentration-dependent increase in cyclic GMP production was observed in HEL299 cells incubated with carbachol, cocaine, or MEG for 24 h. The increase in cyclic GMP content was 3.6 fold for 1 microM carbachol (P < 0.01), 3.1 fold for 1 microM cocaine (P < 0.01), and 7.8 fold for 1 microM MEG (P < 0.001), respectively. Carbachol 104-113 5'-nucleotidase, cytosolic II Homo sapiens 164-167 11159694-5 2001 A concentration-dependent increase in cyclic GMP production was observed in HEL299 cells incubated with carbachol, cocaine, or MEG for 24 h. The increase in cyclic GMP content was 3.6 fold for 1 microM carbachol (P < 0.01), 3.1 fold for 1 microM cocaine (P < 0.01), and 7.8 fold for 1 microM MEG (P < 0.001), respectively. Carbachol 202-211 5'-nucleotidase, cytosolic II Homo sapiens 45-48 11159694-5 2001 A concentration-dependent increase in cyclic GMP production was observed in HEL299 cells incubated with carbachol, cocaine, or MEG for 24 h. The increase in cyclic GMP content was 3.6 fold for 1 microM carbachol (P < 0.01), 3.1 fold for 1 microM cocaine (P < 0.01), and 7.8 fold for 1 microM MEG (P < 0.001), respectively. Carbachol 202-211 5'-nucleotidase, cytosolic II Homo sapiens 164-167 11159694-17 2001 However, the inducible isoform of nitric oxide synthase (iNOS) was induced in the presence of cocaine or carbachol and this induction was significantly attenuated after addition of atropine or methoctramine. Carbachol 105-114 nitric oxide synthase 2 Homo sapiens 57-61 11769308-3 2001 In a network model of area CA3, the time scale for CCH-delta corresponded to the decay constant of the gating variable of the calcium-dependent potassium (K-AHP) current, that of CCH-theta to an intrinsic subthreshold membrane potential oscillation of the pyramidal cells, and that of CCH-gamma to the decay constant of GABAergic inhibitory synaptic potentials onto the pyramidal cells. Carbachol 179-182 carbonic anhydrase 3 Rattus norvegicus 27-30 11769308-3 2001 In a network model of area CA3, the time scale for CCH-delta corresponded to the decay constant of the gating variable of the calcium-dependent potassium (K-AHP) current, that of CCH-theta to an intrinsic subthreshold membrane potential oscillation of the pyramidal cells, and that of CCH-gamma to the decay constant of GABAergic inhibitory synaptic potentials onto the pyramidal cells. Carbachol 179-182 carbonic anhydrase 3 Rattus norvegicus 27-30 11301206-6 2001 Such destruction also abolished; (i) intraseptal carbachol-induced suppression of CA1 population spike, and (ii) stimulation-intensity dependent increase in amplitude, but not frequency, of theta evoked on electrical stimulation in the region of oral part of pontine reticular nucleus. Carbachol 49-58 carbonic anhydrase 1 Rattus norvegicus 82-85 11020447-4 2000 In the present paper, we showed that in 123-1N1 human astrocytoma cells, which express the G-protein-coupled M2, M3, and M5 muscarinic receptors, PKC zeta is activated by carbachol in a concentration-dependent manner, resulting in the translocation of PKC zeta from the cytoplasm to granules in the perinuclear region. Carbachol 171-180 protein kinase C zeta Homo sapiens 146-154 10986289-13 2000 In m1 muscarinic receptor transfected HEK-293 cells, carbachol inhibited insulin-like growth factor-1 stimulated phosphorylation at Ser(473) of endogenous Akt in an atropine-reversible fashion. Carbachol 53-62 insulin like growth factor 1 Homo sapiens 73-101 10986289-13 2000 In m1 muscarinic receptor transfected HEK-293 cells, carbachol inhibited insulin-like growth factor-1 stimulated phosphorylation at Ser(473) of endogenous Akt in an atropine-reversible fashion. Carbachol 53-62 AKT serine/threonine kinase 1 Homo sapiens 155-158 11073886-6 2000 Carbachol significantly attenuated the isoproterenol response in wild-type and Galpha(i3)-null cells but had no effect in Galpha(i2)-null cells. Carbachol 0-9 brain protein I3 Mus musculus 79-88 10945986-1 2000 Stimulation of RBL-2H3 m1 mast cells through the IgE receptor with antigen, or through a G protein-coupled receptor with carbachol, leads to the rapid appearance of phosphothreonine in nonmuscle myosin heavy chain II-A (NMHC-IIA). Carbachol 121-130 myosin heavy chain 9-like 1 Rattus norvegicus 185-218 10945986-1 2000 Stimulation of RBL-2H3 m1 mast cells through the IgE receptor with antigen, or through a G protein-coupled receptor with carbachol, leads to the rapid appearance of phosphothreonine in nonmuscle myosin heavy chain II-A (NMHC-IIA). Carbachol 121-130 myosin heavy chain 9-like 1 Rattus norvegicus 220-228 11139286-12 2000 Activation of the Fas pathway in the Bcl-2 and Bcl-xL transfectants by antibody also inhibited carbachol and EGF responsiveness (i.e., Ca2+ mobilization and/or influx) by 50-60%. Carbachol 95-104 BCL2 apoptosis regulator Homo sapiens 37-42 11139286-12 2000 Activation of the Fas pathway in the Bcl-2 and Bcl-xL transfectants by antibody also inhibited carbachol and EGF responsiveness (i.e., Ca2+ mobilization and/or influx) by 50-60%. Carbachol 95-104 BCL2 like 1 Homo sapiens 47-53 11069606-0 2000 A model of gamma-frequency network oscillations induced in the rat CA3 region by carbachol in vitro. Carbachol 81-90 carbonic anhydrase 3 Rattus norvegicus 67-70 11186246-10 2000 These results suggested that amlexanox may not affect either Ca2+ mobilization or calmodulin activity, although it inhibits myosin light-chain kinase, which may inhibit carbachol-induced contraction. Carbachol 169-178 myosin light chain kinase, smooth muscle Oryctolagus cuniculus 124-149 11090124-11 2000 On normal detrusor in vitro, 4-DAMP and methoctramine caused surmountable antagonism of responses to carbachol with pK(B) values of 9.37+/-0.07 and 6.05+/-0.05 respectively. Carbachol 101-110 AKT serine/threonine kinase 1 Sus scrofa 116-121 11074189-8 2000 Rats with seizures induced by PAG carbachol microinjections exhibited dense c-fos-like immunoreactivity in the dentate gyrus but not the CA(1) or CA(3) regions, amygdala, piriform cortex, perirhinal cortex or hypothalamus. Carbachol 34-43 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 76-81 11122360-4 2000 When administered alone, PACAP (3 pmol) or carbachol (110 pmol) induced an enhancement of REM sleep during 8 h (+61%, n = 8; +70%, n = 5), which was totally prevented by infusion of atropine (290 pmol) for PACAP, or of PACAP6-27 (3 pmol) for carbachol. Carbachol 43-52 adenylate cyclase activating polypeptide 1 Rattus norvegicus 206-211 11122360-4 2000 When administered alone, PACAP (3 pmol) or carbachol (110 pmol) induced an enhancement of REM sleep during 8 h (+61%, n = 8; +70%, n = 5), which was totally prevented by infusion of atropine (290 pmol) for PACAP, or of PACAP6-27 (3 pmol) for carbachol. Carbachol 242-251 adenylate cyclase activating polypeptide 1 Rattus norvegicus 25-30 11233758-5 2000 Carbachol caused an increase in phorbol ester binding and translocation of PKCepsilon, however, these were inhibited only by 100-200 mM ethanol. Carbachol 0-9 protein kinase C epsilon Homo sapiens 75-85 11233758-6 2000 On the other hand, translocation of the atypical PKCzeta to the perinuclear area by carbachol was inhibited by ethanol in a dose-dependent manner (10-100 mM). Carbachol 84-93 protein kinase C zeta Homo sapiens 49-56 11082488-0 2000 GABAergic neurons of the cat dorsal raphe nucleus express c-fos during carbachol-induced active sleep. Carbachol 71-80 Fos proto-oncogene, AP-1 transcription factor subunit Felis catus 58-63 11069954-8 2000 (6) Twenty Hertz activity after orthodromic activation of field CA3 was distributed in the same manner as carbachol-induced beta waves and was generated by a current source in the apical dendrites of CA3. Carbachol 106-115 carbonic anhydrase 3 Homo sapiens 64-67 11069954-8 2000 (6) Twenty Hertz activity after orthodromic activation of field CA3 was distributed in the same manner as carbachol-induced beta waves and was generated by a current source in the apical dendrites of CA3. Carbachol 106-115 carbonic anhydrase 3 Homo sapiens 200-203 11078394-2 2000 Endothelin-1 (ET-1; 0.1-100.0 nM) caused graded tonic contractions with a CK50 (concentration causing response equivalent to 50% of KCl 80 mM) of 3.4 nM and EH (response to highest concentration) of 186 +/- 22, being 40-fold less potent than cholecystokinin-8 (CCK-8), 103-fold more potent than carbachol, but equipotent to ET-3, sarafotoxin S6c (S6c) and IRL 1620. Carbachol 295-304 endothelin-1 Oryctolagus cuniculus 0-12 11050286-3 2000 We found that treatment of the muscle with 2"-Amino-3"-methoxyflavone (PD98059) (10 microM), a specific inhibitor of MAP kinase kinase (MEK), inhibited significantly prostaglandin F(2alpha)- and latanoprost-induced phosphorylation and contraction, but had little effect on those evoked by carbachol. Carbachol 289-298 mitogen-activated protein kinase kinase 7 Homo sapiens 136-139 11027250-4 2000 gamma activity induced by either arterial perfusion or intraparenchymal application of CCh showed a phase reversal across mEC layer II and was reduced or abolished in a spatially localized region by focal infusions of atropine, bicuculline, and CNQX. Carbachol 87-90 chemokine (C-C motif) ligand 28 Mus musculus 122-125 11027250-6 2000 Finally, gamma oscillations recorded at multiple sites across the surface of the mEC using array electrodes during arterial perfusion of CCh demonstrated a decline in synchronization (coherence) as the interelectrode distance increased. Carbachol 137-140 chemokine (C-C motif) ligand 28 Mus musculus 81-84 11027250-8 2000 These results suggest that CCh-induced gamma oscillations in the mEC are mediated through direct muscarinic excitation of a highly localized reciprocal inhibitory-excitatory network located in superficial layers. Carbachol 27-30 chemokine (C-C motif) ligand 28 Mus musculus 65-68 18968085-9 2000 The interaction with propranolol, clonidine, phenylephrine, carbachol and tripeptide fMLP, which hinder adenylate cyclase (AdC) and activate Ca-polyphosphoinisitide (Ca-PPI) signaling system of a cell, initiates structural rearrangements similar to acidic transitions of albumin. Carbachol 60-69 albumin Homo sapiens 271-278 11020447-4 2000 In the present paper, we showed that in 123-1N1 human astrocytoma cells, which express the G-protein-coupled M2, M3, and M5 muscarinic receptors, PKC zeta is activated by carbachol in a concentration-dependent manner, resulting in the translocation of PKC zeta from the cytoplasm to granules in the perinuclear region. Carbachol 171-180 protein kinase C zeta Homo sapiens 252-260 11020447-6 2000 A selective PKC zeta inhibitor peptide (peptide Z) inhibited PKC zeta translocation as well as carbachol-induced DNA synthesis. Carbachol 95-104 protein kinase C zeta Homo sapiens 12-20 11020447-7 2000 Inhibition of both phosphatidylinositol 3-kinase and phospholipase D decreased carbachol-induced [(3)H]thymidine incorporation and blocked carbachol-induced PKC zeta translocation, suggesting an involvement of both pathways in these effects. Carbachol 139-148 protein kinase C zeta Homo sapiens 157-165 11003583-6 2000 Inhibition of the carbachol response by EGF was not altered by phorbol ester-induced downregulation of protein kinase C (PKC) but was enhanced upon PKC activation by a diacylglycerol analog. Carbachol 18-27 epidermal growth factor Homo sapiens 40-43 11003583-1 2000 The effects of epidermal growth factor (EGF) on intracellular calcium ([Ca(2+)](i)) responses to the muscarinic agonist carbachol were studied in a human salivary cell line (HSY). Carbachol 120-129 epidermal growth factor Homo sapiens 15-38 11003583-7 2000 Phosphorylation of mitogen-activated protein kinase (MAP kinase) and inhibition of the carbachol response by EGF were both blocked by the MAP kinase pathway inhibitor PD-98059. Carbachol 87-96 epidermal growth factor Homo sapiens 109-112 11003583-1 2000 The effects of epidermal growth factor (EGF) on intracellular calcium ([Ca(2+)](i)) responses to the muscarinic agonist carbachol were studied in a human salivary cell line (HSY). Carbachol 120-129 epidermal growth factor Homo sapiens 40-43 11003583-8 2000 The results suggest that EGF decreases carbachol-induced Ca(2+) release from internal stores and also exerts a direct inhibitory action on Ca(2+) influx. Carbachol 39-48 epidermal growth factor Homo sapiens 25-28 11003583-2 2000 Carbachol (10(-4) M)-stimulated [Ca(2+)](i) mobilization was inhibited by 40% after 48-h treatment with 5 x 10(-10) M EGF. Carbachol 0-9 epidermal growth factor Homo sapiens 118-121 11003583-9 2000 A decline in muscarinic receptor density may contribute to EGF inhibition of carbachol responsiveness. Carbachol 77-86 epidermal growth factor Homo sapiens 59-62 11003583-3 2000 EGF also reduced carbachol-induced [Ca(2+)](i) in Ca(2+)-free medium and Ca(2+) influx following repletion of extracellular Ca(2+). Carbachol 17-26 epidermal growth factor Homo sapiens 0-3 11017921-3 2000 In this study, we show that numerous inflammatory or contractile agents, including thrombin, histamine, and carbachol, potentiate epidermal growth factor (EGF)-stimulated proliferation of human airway smooth muscle (ASM), thus demonstrating a clear synergy between RTK and GPCR activation. Carbachol 108-117 vomeronasal 1 receptor 17 pseudogene Homo sapiens 273-277 11015310-6 2000 In oesophageal preparations precontracted with carbachol, thrombin produced a dual action i.e. relaxation followed by contraction. Carbachol 47-56 coagulation factor II Rattus norvegicus 58-66 11015294-3 2000 In N1E-115 neuroblastoma cells endogenously expressing the M(1) and M(4) receptor subtypes, MT-7 (0.3 - 3.0 nM) inhibited the carbachol (CCh)-stimulated inositol phosphates accumulation, but failed to affect the CCh-induced inhibition of pituitary adenylate cyclase activating polypeptide (PACAP) 38-stimulated cyclic AMP accumulation. Carbachol 126-135 adenylate cyclase activating polypeptide 1 Mus musculus 290-295 11015294-3 2000 In N1E-115 neuroblastoma cells endogenously expressing the M(1) and M(4) receptor subtypes, MT-7 (0.3 - 3.0 nM) inhibited the carbachol (CCh)-stimulated inositol phosphates accumulation, but failed to affect the CCh-induced inhibition of pituitary adenylate cyclase activating polypeptide (PACAP) 38-stimulated cyclic AMP accumulation. Carbachol 137-140 adenylate cyclase activating polypeptide 1 Mus musculus 290-295 11023534-7 2000 Exposure of HT-29 cells to carbachol, a stable receptor agonist, results in a 10-fold increase in cyclooxygenase-2 (COX-2) protein. Carbachol 27-36 prostaglandin-endoperoxide synthase 2 Homo sapiens 98-114 11023534-7 2000 Exposure of HT-29 cells to carbachol, a stable receptor agonist, results in a 10-fold increase in cyclooxygenase-2 (COX-2) protein. Carbachol 27-36 prostaglandin-endoperoxide synthase 2 Homo sapiens 116-121 10882720-5 2000 Trp1-expressing cells displayed only modest carbachol-induced Ca(2+) entry and lacked OAG-induced Sr(2+) entry, whereas Trp3-expressing cells responded to both agents with a substantial divalent cation entry. Carbachol 44-53 transient receptor potential cation channel subfamily C member 1 Homo sapiens 0-4 11775829-4 2000 RESULTS: Both the muscarinic receptor agonist, carbachol (Carb 10 mumol/L), and anti-peptide antibodies (Abs 100 nmol/L) could decrease basal cAMP levels (by 46.9% +/- 4.2% and 60.2% +/- 4.6%, respectively) and basal Ica. Carbachol 47-56 syntaxin 8 Homo sapiens 58-62 10882720-6 2000 Coexpression of Trp1 plus Trp3 suppressed carbachol-induced Ca(2+) entry compared with Trp3 expression and abolished OAG-induced Sr(2+) entry signals. Carbachol 42-51 transient receptor potential cation channel subfamily C member 1 Homo sapiens 16-20 10882720-6 2000 Coexpression of Trp1 plus Trp3 suppressed carbachol-induced Ca(2+) entry compared with Trp3 expression and abolished OAG-induced Sr(2+) entry signals. Carbachol 42-51 transient receptor potential cation channel subfamily C member 3 Homo sapiens 26-30 10971280-7 2000 RESULTS: The potency of AII was similar in child and adult detrusor strips, with mean (SEM) pD2 values of 6.9 (1.0) (n = 25) and 6.7 (0.2) (n = 9) respectively, and the maximum responses (to 10 micromol/L AII) rather low (39% and 49%, respectively, P > 0.05), compared with carbachol (100 micromol/L). Carbachol 277-286 NLR family pyrin domain containing 3 Homo sapiens 24-27 10956273-6 2000 Bladder strips from cGKI-/- mice responded normally to electrical field stimulation and to carbachol but not to 8-BrcGMP. Carbachol 91-100 protein kinase, cGMP-dependent, type I Mus musculus 20-24 10960062-3 2000 However, there were regional differences in the efficacy of hU-II, with a progressive increase in the maximum contraction from trachea to smaller airway regions (from 9 to 41% of the contraction to 10 microM carbachol). Carbachol 208-217 urotensin 2 Homo sapiens 60-65 10944110-7 2000 Finally, repeated carbachol stimulations of mAchRs co-expressed in COS cells with endothelial nitric oxide synthase (eNOS) and wild-type, but not mutant, dynamin led to a progressive increase in mAchR sequestration and a concurrent stabilization of the inhibitory eNOS-caveolin complex. Carbachol 18-27 nitric oxide synthase 3 Homo sapiens 82-115 10965900-3 2000 Wortmannin amplified insulin release induced by the combination of 6-8 mM glucose plus 1 microM carbachol; however, it had no effect on phorbol ester- or alpha-ketoisocaproate-induced insulin secretion. Carbachol 96-105 insulin Homo sapiens 21-28 10945862-3 2000 The PTX-sensitive GPC mitogens carbachol and endothelin-1 and the PTX-insensitive GPC mitogens sphingosine-1-phosphate and thrombin exhibited synergistic stimulation together with EGF. Carbachol 31-40 glycophorin C (Gerbich blood group) Homo sapiens 18-21 10953050-6 2000 Although RGS14 does not act as a GAP for G12/13alpha, it impairs c-fos serum response element activation induced by either a constitutively active mutant of G13alpha (G13alphaQ226L) or by carbachol stimulation of muscarinic type 1 receptors. Carbachol 188-197 regulator of G-protein signaling 14 Mus musculus 9-14 11032727-7 2000 It was found that cell proliferation, viability, insulin production and the stimulation of insulin release evoked by carbamylcholine and phorbol ester were impeded by IL-1beta or spermine-NONOate, whereas the hormone output by the other secretagogues was not altered by NO. Carbachol 117-132 interleukin 1 beta Rattus norvegicus 167-175 10801833-10 2000 Thus, the inhibitory effect of EGF on carbachol-induced chloride secretion involves the activation of PKCepsilon mediated by PI 3-kinase. Carbachol 38-47 protein kinase C epsilon Homo sapiens 102-112 10924670-7 2000 Our data suggest that activation of CaMKII by carbachol is crucial for local theta-like activity in the CA1 area of the rat hippocampus in vitro. Carbachol 46-55 carbonic anhydrase 1 Rattus norvegicus 104-107 10934239-4 2000 Although carbachol did not enhance ISO-induced increases in cAMP, coapplication of ISO and carbachol synergistically activated p42 mitogen-activated protein kinase (p42 MAPK). Carbachol 91-100 mitogen-activated protein kinase 1 Homo sapiens 127-163 10934239-4 2000 Although carbachol did not enhance ISO-induced increases in cAMP, coapplication of ISO and carbachol synergistically activated p42 mitogen-activated protein kinase (p42 MAPK). Carbachol 91-100 mitogen-activated protein kinase 1 Homo sapiens 165-173 10926555-2 2000 We have shown previously that TNF-alpha upregulates the expression of Galpha(i-2) protein without significantly increasing G(s)alpha protein and enhances adenylyl cyclase inhibition by carbachol in cultured human airway smooth muscle cells (Hotta K, Emala CW, and Hirshman CA. Carbachol 185-194 tumor necrosis factor Homo sapiens 30-39 10801833-1 2000 Epidermal growth factor (EGF) inhibits carbachol-induced chloride secretion in T(84) colonic epithelial cells and has been shown to activate phosphatidylinositol (PI) 3-kinase, leading to inhibition of a basolateral potassium conductance. Carbachol 39-48 epidermal growth factor Homo sapiens 0-23 10801833-1 2000 Epidermal growth factor (EGF) inhibits carbachol-induced chloride secretion in T(84) colonic epithelial cells and has been shown to activate phosphatidylinositol (PI) 3-kinase, leading to inhibition of a basolateral potassium conductance. Carbachol 39-48 epidermal growth factor Homo sapiens 25-28 10787424-8 2000 Adenovirus-directed overexpression of the human beta(2)-AR results in elevated base-line cAMP and contraction associated with a marked attenuation of beta(1)-AR response; carbachol pretreatment fully revives the diminished beta(1)-AR contractile response. Carbachol 171-180 adrenoceptor beta 2 Homo sapiens 48-58 10959486-3 2000 On the other hand, SIN-1 reduced carbachol-induced inositol 1,4,5-trisphosphate (IP3) formation. Carbachol 33-42 MAPK associated protein 1 Homo sapiens 19-24 10787424-8 2000 Adenovirus-directed overexpression of the human beta(2)-AR results in elevated base-line cAMP and contraction associated with a marked attenuation of beta(1)-AR response; carbachol pretreatment fully revives the diminished beta(1)-AR contractile response. Carbachol 171-180 adrenoceptor beta 1 Homo sapiens 150-160 10787424-8 2000 Adenovirus-directed overexpression of the human beta(2)-AR results in elevated base-line cAMP and contraction associated with a marked attenuation of beta(1)-AR response; carbachol pretreatment fully revives the diminished beta(1)-AR contractile response. Carbachol 171-180 adrenoceptor beta 1 Homo sapiens 223-233 10801833-9 2000 Antisense oligonucleotides against PKCepsilon decreased PKCepsilon mass and prevented the inhibitory effect of EGF on carbachol-induced (86)Rb(+) efflux. Carbachol 118-127 epidermal growth factor Homo sapiens 111-114 10801833-10 2000 Thus, the inhibitory effect of EGF on carbachol-induced chloride secretion involves the activation of PKCepsilon mediated by PI 3-kinase. Carbachol 38-47 epidermal growth factor Homo sapiens 31-34 10860637-1 2000 In this study we examined the mechanism of the concentration-dependent relaxant effect of angiotensin II (A II) on mouse isolated tracheal rings precontracted by carbachol. Carbachol 162-171 arginase type II Mus musculus 90-104 10852825-9 2000 Similarly, in RBL-2H3 cells that stably express physiological levels of m1AChR, the addition of carbachol selectively induces the localization of arrestin-3, but not arrestin-2, to coated pits. Carbachol 96-105 arrestin 3 Rattus norvegicus 146-156 10860637-1 2000 In this study we examined the mechanism of the concentration-dependent relaxant effect of angiotensin II (A II) on mouse isolated tracheal rings precontracted by carbachol. Carbachol 162-171 arginase type II Mus musculus 106-110 10860637-6 2000 K(+)-channel blockers partially inhibited the relaxant effect of A II in carbachol-precontracted mouse tracheal rings. Carbachol 73-82 arginase type II Mus musculus 65-69 10860637-9 2000 These results suggest that the mechanisms, involved in the relaxation induced by A II in the isolated mouse tracheal rings precontracted by carbachol, were firstly l -arginine NO and cyclo-oxygenase products of arachidonic acid, secondly K(+)channels. Carbachol 140-149 arginase type II Mus musculus 81-85 10833448-3 2000 We studied whether islet phospholipase A(2) (PLA(2)) contributes by using C57BL/6J mice fed a high-fat diet, since we previously showed that the insulin responses to the two PLA(2)-activating insulin secretagogues carbachol and cholecystokinin (CCK) are enhanced in this model. Carbachol 214-223 phospholipase A2, group IB, pancreas Mus musculus 174-180 10770956-9 2000 Carbachol also activated p38 kinase, and the protective effect of carbachol was abolished by SB-202190. Carbachol 0-9 mitogen activated protein kinase 14 Rattus norvegicus 25-28 10748023-8 2000 Interestingly, NHERF2 potentiated the PLC-beta activation by carbachol in COS7 and HeLa cells, while mutant NHERF2, lacking the second PDZ domain, had no such effect. Carbachol 61-70 SLC9A3 regulator 2 Homo sapiens 15-21 10838169-9 2000 In separate studies that used peptide gamma, CCK-8, CCh and CCK-OPE dose-dependently increased CaMKIV activities. Carbachol 52-55 calcium/calmodulin-dependent protein kinase IV Rattus norvegicus 95-101 10838169-13 2000 CCK-8, CCh and CCK-OPE also significantly increased phosphotransferase activities of myosin light chain kinase (MLCK) substrate (basal: 4.4+/-0.7 pmol/min/mg protein). Carbachol 7-10 myosin light chain kinase Rattus norvegicus 85-110 10838169-13 2000 CCK-8, CCh and CCK-OPE also significantly increased phosphotransferase activities of myosin light chain kinase (MLCK) substrate (basal: 4.4+/-0.7 pmol/min/mg protein). Carbachol 7-10 myosin light chain kinase Rattus norvegicus 112-116 10839927-0 2000 Halothane and isoflurane augment depolarization-induced cytosolic CA2+ transients and attenuate carbachol-stimulated CA2+ transients. Carbachol 96-105 carbonic anhydrase 2 Homo sapiens 117-120 10839927-3 2000 Using a human neuroblastoma cell line, the effects of halothane and isoflurane on cytosolic Ca2+ concentration ([Ca2+]cyt) in response to K+ and carbachol stimulation were investigated. Carbachol 145-154 carbonic anhydrase 2 Homo sapiens 92-95 10839927-3 2000 Using a human neuroblastoma cell line, the effects of halothane and isoflurane on cytosolic Ca2+ concentration ([Ca2+]cyt) in response to K+ and carbachol stimulation were investigated. Carbachol 145-154 carbonic anhydrase 2 Homo sapiens 113-116 10839927-8 2000 In contrast, halothane and isoflurane reduced the carbachol-evoked [Ca2+]cyt transient when the intracellular Ca2+ stores were full but had no effect when the Ca2+ stores were partially depleted by KCl stimulation. Carbachol 50-59 carbonic anhydrase 2 Homo sapiens 68-71 10839927-8 2000 In contrast, halothane and isoflurane reduced the carbachol-evoked [Ca2+]cyt transient when the intracellular Ca2+ stores were full but had no effect when the Ca2+ stores were partially depleted by KCl stimulation. Carbachol 50-59 carbonic anhydrase 2 Homo sapiens 110-113 10839927-8 2000 In contrast, halothane and isoflurane reduced the carbachol-evoked [Ca2+]cyt transient when the intracellular Ca2+ stores were full but had no effect when the Ca2+ stores were partially depleted by KCl stimulation. Carbachol 50-59 carbonic anhydrase 2 Homo sapiens 110-113 10839927-9 2000 CONCLUSIONS: Volatile anesthetics acted on sites that differently affect the K+- and carbachol-evoked [Ca2+]cyt transients. Carbachol 85-94 carbonic anhydrase 2 Homo sapiens 103-106 10816433-1 2000 The acetylcholine analogue carbachol rapidly activated mitogen-activated protein kinase (MAPK), and caused tyrosine phosphorylation of the adapter protein p52 Shc and the epidermalgrowth factor (EGF) receptor, in human embryonic kidney cells stably expressing m3 muscarinic receptors. Carbachol 27-36 SHC adaptor protein 1 Homo sapiens 159-162 10816433-1 2000 The acetylcholine analogue carbachol rapidly activated mitogen-activated protein kinase (MAPK), and caused tyrosine phosphorylation of the adapter protein p52 Shc and the epidermalgrowth factor (EGF) receptor, in human embryonic kidney cells stably expressing m3 muscarinic receptors. Carbachol 27-36 epidermal growth factor receptor Homo sapiens 171-208 10816433-3 2000 The PKC-independent MAPK activity elicited by carbachol in the presence of GF109203X was reproducibly abolished by AG1478, an inhibitor of EGF-receptor tyrosine kinase activity, and by the Src tyrosine kinase inhibitor PP1. Carbachol 46-55 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 189-192 10816433-3 2000 The PKC-independent MAPK activity elicited by carbachol in the presence of GF109203X was reproducibly abolished by AG1478, an inhibitor of EGF-receptor tyrosine kinase activity, and by the Src tyrosine kinase inhibitor PP1. Carbachol 46-55 neuropeptide Y receptor Y4 Homo sapiens 219-222 10816433-4 2000 In a subset of these experiments, GF109203X concomitantly increased carbachol-induced tyrosine phosphorylation of p52 Shc and the EGF receptor. Carbachol 68-77 SHC adaptor protein 1 Homo sapiens 118-121 10816433-4 2000 In a subset of these experiments, GF109203X concomitantly increased carbachol-induced tyrosine phosphorylation of p52 Shc and the EGF receptor. Carbachol 68-77 epidermal growth factor receptor Homo sapiens 130-142 10816433-5 2000 In co-stimulation experiments, carbachol and EGF activated MAPK in a non-additive fashion; moreover, EGF-induced association of Shc with the phosphorylated EGF receptor was inhibited by carbachol. Carbachol 31-40 SHC adaptor protein 1 Homo sapiens 128-131 10816433-5 2000 In co-stimulation experiments, carbachol and EGF activated MAPK in a non-additive fashion; moreover, EGF-induced association of Shc with the phosphorylated EGF receptor was inhibited by carbachol. Carbachol 31-40 epidermal growth factor receptor Homo sapiens 156-168 10816433-5 2000 In co-stimulation experiments, carbachol and EGF activated MAPK in a non-additive fashion; moreover, EGF-induced association of Shc with the phosphorylated EGF receptor was inhibited by carbachol. Carbachol 186-195 SHC adaptor protein 1 Homo sapiens 128-131 10816433-5 2000 In co-stimulation experiments, carbachol and EGF activated MAPK in a non-additive fashion; moreover, EGF-induced association of Shc with the phosphorylated EGF receptor was inhibited by carbachol. Carbachol 186-195 epidermal growth factor receptor Homo sapiens 156-168 10936763-2 2000 Carbachol stimulated gastrin release in a dose-dependent manner but had no effect on somatostatin release. Carbachol 0-9 gastrin Homo sapiens 21-28 10936763-3 2000 As atropine blocked the effect of carbachol, cholinergic agonists appear to stimulate gastrin secretion directly through muscarinic receptors on the G-cell and not by inhibition of somatostatin secretion. Carbachol 34-43 gastrin Homo sapiens 86-93 11014771-0 2000 Carbachol potentiates cholera toxin-induced secretion in a colonic epithelial cell line (HT29-19A) and rat ileal mucosa in vitro. Carbachol 0-9 SLAM family member 7 Homo sapiens 94-97 11014771-5 2000 Pre-treatment of the HT29-19A cell lines with carbachol potentiated cholera toxin-induced secretory response, and enhanced accumulation of cAMP. Carbachol 46-55 SLAM family member 7 Homo sapiens 26-29 10848561-9 2000 Verruculogen (100 nM), carbachol (100 microM), apamin (100 nM), TEA (1 mM), and iberiotoxin (50 nM) significantly reduced early I(K(Ca)) on CA1 pyramidal neurons; early I(K(Ca)) on L-M interneurons was inhibited by apamin and TEA. Carbachol 23-32 carbonic anhydrase 1 Rattus norvegicus 140-143 10833448-4 2000 CCK (100 nM) and carbachol (100 microM) stimulated [(3)H]AA efflux, reflecting PLA(2) activation, both in islets from mice after 12 weeks on high-fat diet and in controls. Carbachol 17-26 phospholipase A2, group IB, pancreas Mus musculus 79-85 10775445-4 2000 Results show that stimulation of pancreatic acini with carbachol resulted in a rapid and transient increase in tyrosine phosphorylation of p125(FAK), p130(cas), and paxillin. Carbachol 55-64 protein tyrosine kinase 2 Rattus norvegicus 144-147 10799557-7 2000 Interestingly, treatment of acini from AC8-KO mice with agents, i.e. carbachol and thapsigargin that increase intracellular Ca(2+), lowered cAMP levels. Carbachol 69-78 adenylate cyclase 8 Mus musculus 39-42 10775445-4 2000 Results show that stimulation of pancreatic acini with carbachol resulted in a rapid and transient increase in tyrosine phosphorylation of p125(FAK), p130(cas), and paxillin. Carbachol 55-64 phospholipase C-like 1 Rattus norvegicus 150-154 10775445-7 2000 Pretreatment of pancreatic acini with Clostridium botulinum C3 transferase, which specifically inactivates p21(rho), partially inhibited carbachol-induced p125(FAK), p130(cas), and paxillin tyrosine phosphorylation. Carbachol 137-146 KRAS proto-oncogene, GTPase Rattus norvegicus 107-110 10775445-7 2000 Pretreatment of pancreatic acini with Clostridium botulinum C3 transferase, which specifically inactivates p21(rho), partially inhibited carbachol-induced p125(FAK), p130(cas), and paxillin tyrosine phosphorylation. Carbachol 137-146 protein tyrosine kinase 2 Rattus norvegicus 160-163 10775445-7 2000 Pretreatment of pancreatic acini with Clostridium botulinum C3 transferase, which specifically inactivates p21(rho), partially inhibited carbachol-induced p125(FAK), p130(cas), and paxillin tyrosine phosphorylation. Carbachol 137-146 phospholipase C-like 1 Rattus norvegicus 166-170 10813386-2 2000 Pretreatment with C3 exoenzyme of Clostridium botulinum, which selectively inactivates rho p21 by adenosine diphosphate (ADP) ribosylation, resulted in a significant inhibition of ET-1-induced Ca2+ sensitization, but had no effect on carbachol-induced Ca2+ sensitization. Carbachol 234-243 endothelin-1 Oryctolagus cuniculus 180-184 10777795-1 2000 In the present study, we report that the cuneiform (Cun) nucleus, a brainstem structure that before now has not been implicated in sleep processes, exhibits a large number of neurons that express c-fos during carbachol-induced active sleep (AS-carbachol). Carbachol 209-218 Fos proto-oncogene, AP-1 transcription factor subunit Felis catus 196-201 10959486-1 2000 We have investigated the effect of 3-morpholinosydnonimine (SIN-1), a peroxynitrite donor, on carbachol-induced increase in intracellular Ca2+ concentration ([Ca2+]i) in human neuroblastoma SH-SY5Y cells by means of single cell imaging of [Ca2+]i. Carbachol 94-103 MAPK associated protein 1 Homo sapiens 60-65 10959486-2 2000 SIN-1 potentiated carbachol-induced [Ca2+]i rise regardless of external Ca2+, and the potentiation was completely inhibited by superoxide dismutase, indicating that peroxynitrite may enhance Ca2+ release from intracellular stores. Carbachol 18-27 MAPK associated protein 1 Homo sapiens 0-5 10800946-3 2000 The carbachol-induced arachidonate release is potentiated two- to threefold by pretreatment of A2058 cells with either of the inflammatory cytokines, tumor necrosis factor-alpha or interleukin-1beta . Carbachol 4-13 tumor necrosis factor Homo sapiens 150-177 10800946-3 2000 The carbachol-induced arachidonate release is potentiated two- to threefold by pretreatment of A2058 cells with either of the inflammatory cytokines, tumor necrosis factor-alpha or interleukin-1beta . Carbachol 4-13 interleukin 1 beta Homo sapiens 181-198 10777553-8 2000 CCh also induced tyrosine phosphorylation of p60(src) and association of p60(src) with both PYK-2 and the EGFR. Carbachol 0-3 protein tyrosine kinase 2 beta Homo sapiens 92-97 10777553-9 2000 The Src family kinase inhibitor, PP2 (20 nM-20 microM) attenuated CCh-stimulated EGFR and ERK phosphorylation and potentiated chloride secretory responses to CCh. Carbachol 66-69 neuropeptide Y receptor Y6 (pseudogene) Homo sapiens 33-36 10777553-0 2000 Carbachol-stimulated transactivation of epidermal growth factor receptor and mitogen-activated protein kinase in T(84) cells is mediated by intracellular Ca2+, PYK-2, and p60(src). Carbachol 0-9 epidermal growth factor receptor Homo sapiens 40-72 10777553-0 2000 Carbachol-stimulated transactivation of epidermal growth factor receptor and mitogen-activated protein kinase in T(84) cells is mediated by intracellular Ca2+, PYK-2, and p60(src). Carbachol 0-9 protein tyrosine kinase 2 beta Homo sapiens 160-165 10777553-0 2000 Carbachol-stimulated transactivation of epidermal growth factor receptor and mitogen-activated protein kinase in T(84) cells is mediated by intracellular Ca2+, PYK-2, and p60(src). Carbachol 0-9 sequestosome 1 Homo sapiens 171-174 10777553-9 2000 The Src family kinase inhibitor, PP2 (20 nM-20 microM) attenuated CCh-stimulated EGFR and ERK phosphorylation and potentiated chloride secretory responses to CCh. Carbachol 66-69 epidermal growth factor receptor Homo sapiens 81-85 10777553-0 2000 Carbachol-stimulated transactivation of epidermal growth factor receptor and mitogen-activated protein kinase in T(84) cells is mediated by intracellular Ca2+, PYK-2, and p60(src). Carbachol 0-9 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 175-178 10777553-9 2000 The Src family kinase inhibitor, PP2 (20 nM-20 microM) attenuated CCh-stimulated EGFR and ERK phosphorylation and potentiated chloride secretory responses to CCh. Carbachol 66-69 mitogen-activated protein kinase 1 Homo sapiens 90-93 10777553-1 2000 Ca(2+)-dependent agonists, such as carbachol (CCh), stimulate epidermal growth factor receptor (EGFR) transactivation and mitogen-activated protein kinase activation in T(84) intestinal epithelial cells. Carbachol 35-44 epidermal growth factor receptor Homo sapiens 62-94 10777553-1 2000 Ca(2+)-dependent agonists, such as carbachol (CCh), stimulate epidermal growth factor receptor (EGFR) transactivation and mitogen-activated protein kinase activation in T(84) intestinal epithelial cells. Carbachol 35-44 epidermal growth factor receptor Homo sapiens 96-100 10779394-3 2000 Studies to test whether the selective enhancement in beta-adrenergic receptor (AR) response might result from inhibition of AC6 by Galpha(i) and Gbetagamma indicated that pertussis toxin-sensitive inhibition by the muscarinic cholinergic agonist carbachol was unaltered in myocytes overexpressing AC6. Carbachol 246-255 adenylate cyclase 6 Homo sapiens 124-127 10777553-9 2000 The Src family kinase inhibitor, PP2 (20 nM-20 microM) attenuated CCh-stimulated EGFR and ERK phosphorylation and potentiated chloride secretory responses to CCh. Carbachol 158-161 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 4-7 10777553-1 2000 Ca(2+)-dependent agonists, such as carbachol (CCh), stimulate epidermal growth factor receptor (EGFR) transactivation and mitogen-activated protein kinase activation in T(84) intestinal epithelial cells. Carbachol 46-49 epidermal growth factor receptor Homo sapiens 62-94 10777553-1 2000 Ca(2+)-dependent agonists, such as carbachol (CCh), stimulate epidermal growth factor receptor (EGFR) transactivation and mitogen-activated protein kinase activation in T(84) intestinal epithelial cells. Carbachol 46-49 epidermal growth factor receptor Homo sapiens 96-100 10777553-9 2000 The Src family kinase inhibitor, PP2 (20 nM-20 microM) attenuated CCh-stimulated EGFR and ERK phosphorylation and potentiated chloride secretory responses to CCh. Carbachol 158-161 neuropeptide Y receptor Y6 (pseudogene) Homo sapiens 33-36 10777553-3 2000 Here, we investigated mechanisms underlying CCh-stimulated epidermal growth factor receptor (EGFR) transactivation. Carbachol 44-47 epidermal growth factor receptor Homo sapiens 59-91 10777553-3 2000 Here, we investigated mechanisms underlying CCh-stimulated epidermal growth factor receptor (EGFR) transactivation. Carbachol 44-47 epidermal growth factor receptor Homo sapiens 93-97 10777553-6 2000 CCh (100 microM) stimulated tyrosine phosphorylation and association of the Ca(2+)-dependent tyrosine kinase, PYK-2, with the EGFR, which was inhibited by the Ca(2+) chelator, BAPTA (20 microM). Carbachol 0-3 protein tyrosine kinase 2 beta Homo sapiens 110-115 10777553-6 2000 CCh (100 microM) stimulated tyrosine phosphorylation and association of the Ca(2+)-dependent tyrosine kinase, PYK-2, with the EGFR, which was inhibited by the Ca(2+) chelator, BAPTA (20 microM). Carbachol 0-3 epidermal growth factor receptor Homo sapiens 126-130 10777553-7 2000 The calmodulin inhibitor, fluphenazine (50 microM) inhibited CCh-stimulated PYK-2 association with the EGFR and phosphorylation of EGFR and ERK. Carbachol 61-64 calmodulin 1 Homo sapiens 4-14 10777553-10 2000 We conclude that CCh-stimulated transactivation of the EGFR is mediated by a pathway involving elevations in intracellular Ca(2+), calmodulin, PYK-2, and p60(src). Carbachol 17-20 epidermal growth factor receptor Homo sapiens 55-59 10777553-10 2000 We conclude that CCh-stimulated transactivation of the EGFR is mediated by a pathway involving elevations in intracellular Ca(2+), calmodulin, PYK-2, and p60(src). Carbachol 17-20 calmodulin 1 Homo sapiens 131-141 10777553-10 2000 We conclude that CCh-stimulated transactivation of the EGFR is mediated by a pathway involving elevations in intracellular Ca(2+), calmodulin, PYK-2, and p60(src). Carbachol 17-20 protein tyrosine kinase 2 beta Homo sapiens 143-148 10777553-10 2000 We conclude that CCh-stimulated transactivation of the EGFR is mediated by a pathway involving elevations in intracellular Ca(2+), calmodulin, PYK-2, and p60(src). Carbachol 17-20 sequestosome 1 Homo sapiens 154-157 10777553-10 2000 We conclude that CCh-stimulated transactivation of the EGFR is mediated by a pathway involving elevations in intracellular Ca(2+), calmodulin, PYK-2, and p60(src). Carbachol 17-20 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 158-161 10777553-7 2000 The calmodulin inhibitor, fluphenazine (50 microM) inhibited CCh-stimulated PYK-2 association with the EGFR and phosphorylation of EGFR and ERK. Carbachol 61-64 protein tyrosine kinase 2 beta Homo sapiens 76-81 10758335-3 2000 The results showed that (1) simultaneous incubation with carbachol dose- and time-dependently blocked the specific DNA ladder formation induced by exposure to A(beta31-35) and (2) the A(beta31-35)-induced downregulation of bcl-2 and upregulations of bax, p53, and c-fos genes were reversed or ameliorated by the coadministration of carbachol. Carbachol 57-66 B cell leukemia/lymphoma 2 Mus musculus 223-228 10777553-7 2000 The calmodulin inhibitor, fluphenazine (50 microM) inhibited CCh-stimulated PYK-2 association with the EGFR and phosphorylation of EGFR and ERK. Carbachol 61-64 epidermal growth factor receptor Homo sapiens 103-107 10777553-7 2000 The calmodulin inhibitor, fluphenazine (50 microM) inhibited CCh-stimulated PYK-2 association with the EGFR and phosphorylation of EGFR and ERK. Carbachol 61-64 epidermal growth factor receptor Homo sapiens 131-135 10777553-7 2000 The calmodulin inhibitor, fluphenazine (50 microM) inhibited CCh-stimulated PYK-2 association with the EGFR and phosphorylation of EGFR and ERK. Carbachol 61-64 mitogen-activated protein kinase 1 Homo sapiens 140-143 10777553-8 2000 CCh also induced tyrosine phosphorylation of p60(src) and association of p60(src) with both PYK-2 and the EGFR. Carbachol 0-3 sequestosome 1 Homo sapiens 45-48 10777553-8 2000 CCh also induced tyrosine phosphorylation of p60(src) and association of p60(src) with both PYK-2 and the EGFR. Carbachol 0-3 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 49-52 10777553-8 2000 CCh also induced tyrosine phosphorylation of p60(src) and association of p60(src) with both PYK-2 and the EGFR. Carbachol 0-3 sequestosome 1 Homo sapiens 73-76 10777553-8 2000 CCh also induced tyrosine phosphorylation of p60(src) and association of p60(src) with both PYK-2 and the EGFR. Carbachol 0-3 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 77-80 10777553-8 2000 CCh also induced tyrosine phosphorylation of p60(src) and association of p60(src) with both PYK-2 and the EGFR. Carbachol 0-3 epidermal growth factor receptor Homo sapiens 106-110 10777553-9 2000 The Src family kinase inhibitor, PP2 (20 nM-20 microM) attenuated CCh-stimulated EGFR and ERK phosphorylation and potentiated chloride secretory responses to CCh. Carbachol 66-69 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 4-7 10751428-4 2000 Tubulin, at nanomolar concentrations, transactivated Galphaq by the direct transfer of a GTP analog and potentiated carbachol-activated PLCbeta(1). Carbachol 116-125 phospholipase C beta 1 Homo sapiens 136-146 10751321-7 2000 Carbachol stimulation increased caldesmon phosphorylation at Ser789 in intact tracheal smooth muscle, which was blocked by the M(2) antagonist AF-DX 116 (1 microM). Carbachol 0-9 caldesmon 1 Homo sapiens 32-41 10840221-6 2000 In the colon, while SST inhibited the carbachol induced increase in I(sc), pre-treatment with tetrodotoxin (750 nM) profoundly inhibited the carbachol induced increase in I(sc), thus markedly reducing the inhibitory effect of SST. Carbachol 38-47 somatostatin Mus musculus 20-23 10840221-6 2000 In the colon, while SST inhibited the carbachol induced increase in I(sc), pre-treatment with tetrodotoxin (750 nM) profoundly inhibited the carbachol induced increase in I(sc), thus markedly reducing the inhibitory effect of SST. Carbachol 141-150 somatostatin Mus musculus 226-229 10752952-1 2000 PURPOSE: To determine the cholinergic (carbachol, CCH) and adrenergic (norepinephrine, NE) modulation of Ca2+ response to endothelin-1 in human ciliary smooth muscle (HCSM) cells. Carbachol 39-48 endothelin 1 Homo sapiens 122-134 10752952-5 2000 Carbachol also dose-dependently increased [Ca2+]i; however, subsequent additions of ET-1 (200 nM) resulted in lower [Ca2+]i (100 microM CCH + ET-1; 300 +/- 21 nM) compared with that observed with 200 nM ET-1 alone (2564 +/- 359 nM). Carbachol 0-9 endothelin 1 Homo sapiens 84-88 10752952-5 2000 Carbachol also dose-dependently increased [Ca2+]i; however, subsequent additions of ET-1 (200 nM) resulted in lower [Ca2+]i (100 microM CCH + ET-1; 300 +/- 21 nM) compared with that observed with 200 nM ET-1 alone (2564 +/- 359 nM). Carbachol 0-9 endothelin 1 Homo sapiens 142-146 10752952-5 2000 Carbachol also dose-dependently increased [Ca2+]i; however, subsequent additions of ET-1 (200 nM) resulted in lower [Ca2+]i (100 microM CCH + ET-1; 300 +/- 21 nM) compared with that observed with 200 nM ET-1 alone (2564 +/- 359 nM). Carbachol 0-9 endothelin 1 Homo sapiens 142-146 10752933-8 2000 Similarly, cAMP increased by 9%, 70%, and 210% in response to 10(-9), 10(-7), and 10(-5) M carbachol, respectively. Carbachol 91-100 cathelicidin antimicrobial peptide Homo sapiens 11-15 10752933-9 2000 In addition, cAMP levels significantly increased by 39% in isolated TM strips incubated with 10(-7) M carbachol. Carbachol 102-111 cathelicidin antimicrobial peptide Homo sapiens 13-17 10702271-7 2000 Activation of Pyk2 via the muscarinic and nicotinic receptors using carbachol or via intracellular Ca(2+) rise using ionomycin/phorbol myristate acetate promoted survival in the absence of IL-7. Carbachol 68-77 PTK2 protein tyrosine kinase 2 beta Mus musculus 14-18 10758335-3 2000 The results showed that (1) simultaneous incubation with carbachol dose- and time-dependently blocked the specific DNA ladder formation induced by exposure to A(beta31-35) and (2) the A(beta31-35)-induced downregulation of bcl-2 and upregulations of bax, p53, and c-fos genes were reversed or ameliorated by the coadministration of carbachol. Carbachol 57-66 BCL2-associated X protein Mus musculus 250-253 10758335-3 2000 The results showed that (1) simultaneous incubation with carbachol dose- and time-dependently blocked the specific DNA ladder formation induced by exposure to A(beta31-35) and (2) the A(beta31-35)-induced downregulation of bcl-2 and upregulations of bax, p53, and c-fos genes were reversed or ameliorated by the coadministration of carbachol. Carbachol 57-66 transformation related protein 53, pseudogene Mus musculus 255-258 10683200-2 2000 VIP induced a concentration-dependent inhibition of carbachol-induced contraction in smooth muscle cells with a maximum at 10(-6) M. The relaxation by 10(-6) M VIP was inhibited for 79.1+/-5.8% (mean+/-s.e. Carbachol 52-61 VIP peptides Cavia porcellus 0-3 10712234-7 2000 However, the secretory response to the muscarinic receptor agonist carbachol was strongly increased after exposure to TNF-alpha. Carbachol 67-76 tumor necrosis factor Homo sapiens 118-127 10712234-8 2000 Application of the protein kinase C (PKC) inhibitor GF 109203X (bisindolylmaleimide I) inhibited the response to carbachol as well as the TNF-alpha-potentiated response, indicating that PKC mediates the effect of carbachol in this cell line. Carbachol 213-222 tumor necrosis factor Homo sapiens 138-147 10704824-3 2000 Addition of carbachol or CCK-8 to pancreatic acini resulted in rapid increases in the tyrosine phosphorylation of p125(FAK), p130(Cas), and paxillin. Carbachol 12-21 protein tyrosine kinase 2 Rattus norvegicus 119-122 10704824-3 2000 Addition of carbachol or CCK-8 to pancreatic acini resulted in rapid increases in the tyrosine phosphorylation of p125(FAK), p130(Cas), and paxillin. Carbachol 12-21 phospholipase C-like 1 Rattus norvegicus 125-129 10758335-3 2000 The results showed that (1) simultaneous incubation with carbachol dose- and time-dependently blocked the specific DNA ladder formation induced by exposure to A(beta31-35) and (2) the A(beta31-35)-induced downregulation of bcl-2 and upregulations of bax, p53, and c-fos genes were reversed or ameliorated by the coadministration of carbachol. Carbachol 57-66 FBJ osteosarcoma oncogene Mus musculus 264-269 10758335-3 2000 The results showed that (1) simultaneous incubation with carbachol dose- and time-dependently blocked the specific DNA ladder formation induced by exposure to A(beta31-35) and (2) the A(beta31-35)-induced downregulation of bcl-2 and upregulations of bax, p53, and c-fos genes were reversed or ameliorated by the coadministration of carbachol. Carbachol 332-341 B cell leukemia/lymphoma 2 Mus musculus 223-228 10670466-3 2000 RESULTS: Preactivation of PKC by phorbol 12-myristate 13-acetate (PMA), or inhibition of protein phosphatase type 1/2A (PP1/2A) by calyculin A, decreased both the [Ca2+]i transient and the plateau of [Ca2+]i induced by increasing concentrations of carbachol, a cholinergic agonist. Carbachol 248-257 neuropeptide Y receptor Y4 Rattus norvegicus 120-126 10676879-3 2000 Exposure of granule cells from wild-type or tenascin-C-negative mice to the muscarinic acetylcholine receptor agonist carbachol (1 mM) resulted in normal sequestration of cell-surface muscarinic acetylcholine receptors as assessed by [3H]N-methylscopolamine binding; however, down-regulation of total muscarinic acetylcholine receptors, measured with [3H]quinuclidinyl benzilate, was inhibited in granule cells from tenascin-C-negative mice. Carbachol 118-127 tenascin C Mus musculus 44-52 10676879-3 2000 Exposure of granule cells from wild-type or tenascin-C-negative mice to the muscarinic acetylcholine receptor agonist carbachol (1 mM) resulted in normal sequestration of cell-surface muscarinic acetylcholine receptors as assessed by [3H]N-methylscopolamine binding; however, down-regulation of total muscarinic acetylcholine receptors, measured with [3H]quinuclidinyl benzilate, was inhibited in granule cells from tenascin-C-negative mice. Carbachol 118-127 tenascin C Mus musculus 416-424 10685865-4 2000 Stimulation with low concentrations of carbachol or cholecystokinin octapeptide (CCK-8) induces [Ca2+]i oscillations whereas higher concentrations cause sustained elevation of [Ca2+]i. Carbachol 39-48 cholecystokinin Cavia porcellus 81-84 10786716-5 2000 Prolonged exposure to a sublethal dose of A(beta) 25-35 or 1-42 disrupted carbachol-mediated release of Ins(1,4,5)P3 and [Ca2+]i, which was inhibited in media supplemented with B27 or the antioxidant vitamin E. Carbachol 74-83 amyloid beta precursor protein Rattus norvegicus 42-49 10636879-1 2000 In HEK 293 cells stably expressing type 1 parathyroid (PTH) receptors, PTH stimulated release of intracellular Ca(2+) stores in only 27% of cells, whereas 96% of cells responded to carbachol. Carbachol 181-190 parathyroid hormone Homo sapiens 55-58 10636879-2 2000 However, in almost all cells PTH potentiated the response to carbachol by about 3-fold. Carbachol 61-70 parathyroid hormone Homo sapiens 29-32 10636879-6 2000 Intracellular heparin inhibited responses to carbachol and PTH, and pretreatment with ATP and carbachol abolished responses to PTH, suggesting that the effects of PTH involve inositol trisphosphate (IP(3)) receptors. Carbachol 94-103 parathyroid hormone Homo sapiens 127-130 10636879-6 2000 Intracellular heparin inhibited responses to carbachol and PTH, and pretreatment with ATP and carbachol abolished responses to PTH, suggesting that the effects of PTH involve inositol trisphosphate (IP(3)) receptors. Carbachol 94-103 parathyroid hormone Homo sapiens 127-130 10683200-2 2000 VIP induced a concentration-dependent inhibition of carbachol-induced contraction in smooth muscle cells with a maximum at 10(-6) M. The relaxation by 10(-6) M VIP was inhibited for 79.1+/-5.8% (mean+/-s.e. Carbachol 52-61 VIP peptides Cavia porcellus 160-163 10652198-5 2000 The potency and efficacy to carbachol in the presence of (-)-isoprenaline were higher in right atria from mdx compared to C57 mice (P<0.05), although in left atria only a greater efficacy was evident in mdx mice. Carbachol 28-37 dystrophin, muscular dystrophy Mus musculus 106-109 10651876-5 2000 Both Ca2+ and PKC responses were observed within seconds following KCl or carbachol application, and were reversible upon stimulus withdrawal. Carbachol 74-83 protein kinase C, gamma Rattus norvegicus 14-17 10902895-11 2000 2) Carbachol induces IPSC activity that can be recorded in CA1 and CA3a. Carbachol 3-12 carbonic anhydrase 1 Rattus norvegicus 59-62 10652198-5 2000 The potency and efficacy to carbachol in the presence of (-)-isoprenaline were higher in right atria from mdx compared to C57 mice (P<0.05), although in left atria only a greater efficacy was evident in mdx mice. Carbachol 28-37 dystrophin, muscular dystrophy Mus musculus 206-209 11129110-5 2000 In neurons a nonspecific muscarinic agonist, carbachol, reduced tau phosphorylation. Carbachol 45-54 microtubule associated protein tau Homo sapiens 64-67 10791841-1 2000 Carbachol, a muscarinic receptor agonist, produced three distinct spontaneous oscillations in the CA3 region of rat hippocampal slices. Carbachol 0-9 carbonic anhydrase 3 Rattus norvegicus 98-101 10601308-4 1999 Co-expression of Ras-GRF1 with subtype 1 human muscarinic receptors in COS-7 cells allowed mapping of a carbachol-stimulated phosphorylation site to a region composed of residues 916-976. Carbachol 104-113 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 17-25 10791841-2 2000 Carbachol concentrations in the 4-13 microM range produced regular synchronized CA3 discharges at 0.5-2 Hz (carbachol-delta). Carbachol 0-9 carbonic anhydrase 3 Rattus norvegicus 80-83 10791841-2 2000 Carbachol concentrations in the 4-13 microM range produced regular synchronized CA3 discharges at 0.5-2 Hz (carbachol-delta). Carbachol 108-117 carbonic anhydrase 3 Rattus norvegicus 80-83 10791841-8 2000 Field and intracellular recordings from CA1 and CA3 pyramidal cells and interneurons during carbachol-induced rhythms revealed that the hippocampal circuitry preserved in the slice was capable of spontaneous activity over the range of frequencies observed in vivo and suggests that the presence of these rhythms could be under neuromodulatory control. Carbachol 92-101 carbonic anhydrase 1 Rattus norvegicus 40-43 10791841-8 2000 Field and intracellular recordings from CA1 and CA3 pyramidal cells and interneurons during carbachol-induced rhythms revealed that the hippocampal circuitry preserved in the slice was capable of spontaneous activity over the range of frequencies observed in vivo and suggests that the presence of these rhythms could be under neuromodulatory control. Carbachol 92-101 carbonic anhydrase 3 Rattus norvegicus 48-51 10794846-6 2000 In contrast to phosphoinositide hydrolysis, carbachol-stimulated AP-1 DNA binding activity was lower in Alzheimer"s disease than control cybrid cells, and this deficit was associated with deficient protein kinase C-mediated activation of AP-1. Carbachol 44-53 FosB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 65-69 10794846-6 2000 In contrast to phosphoinositide hydrolysis, carbachol-stimulated AP-1 DNA binding activity was lower in Alzheimer"s disease than control cybrid cells, and this deficit was associated with deficient protein kinase C-mediated activation of AP-1. Carbachol 44-53 FosB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 238-242 10622253-2 1999 The transactivation of epidermal growth factor receptor (EGFR)-dependent signalling pathways upon stimulation of G-protein-coupled receptors (GPCRs), which are critical for the mitogenic activity of ligands such as lysophosphatidic acid, endothelin, thrombin, bombesin and carbachol, provides evidence for such an interconnected communication network. Carbachol 273-282 epidermal growth factor receptor Homo sapiens 23-55 10622253-2 1999 The transactivation of epidermal growth factor receptor (EGFR)-dependent signalling pathways upon stimulation of G-protein-coupled receptors (GPCRs), which are critical for the mitogenic activity of ligands such as lysophosphatidic acid, endothelin, thrombin, bombesin and carbachol, provides evidence for such an interconnected communication network. Carbachol 273-282 epidermal growth factor receptor Homo sapiens 57-61 10622253-2 1999 The transactivation of epidermal growth factor receptor (EGFR)-dependent signalling pathways upon stimulation of G-protein-coupled receptors (GPCRs), which are critical for the mitogenic activity of ligands such as lysophosphatidic acid, endothelin, thrombin, bombesin and carbachol, provides evidence for such an interconnected communication network. Carbachol 273-282 coagulation factor II, thrombin Homo sapiens 250-258 10622253-2 1999 The transactivation of epidermal growth factor receptor (EGFR)-dependent signalling pathways upon stimulation of G-protein-coupled receptors (GPCRs), which are critical for the mitogenic activity of ligands such as lysophosphatidic acid, endothelin, thrombin, bombesin and carbachol, provides evidence for such an interconnected communication network. Carbachol 273-282 gastrin releasing peptide Homo sapiens 260-268 10601308-8 1999 Full-length Ras-GRF1 that contains an alanine 916 mutation was only partially activated by carbachol, suggesting that phosphorylation at residue 916 is necessary for full activation. Carbachol 91-100 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 12-20 10602325-2 1999 The dose-response parameters of recombinant mouse adult neuromuscular acetylcholine receptor channels (nAChR) activated by carbamylcholine, nicotine, muscarine and oxotremorine were measured. Carbachol 123-138 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 103-108 10600935-7 1999 CO (100 nM) also suppressed NO release induced by 100 microM carbachol, a potent agonist for endothelial NOS (eNOS). Carbachol 61-70 nitric oxide synthase 3 Rattus norvegicus 93-108 10559227-2 1999 The muscarinic agonist of chloride secretion, carbachol (CCh), also stimulates an antisecretory pathway that involves transactivation of the EGF receptor (EGFR) but does not involve PI3-K. Carbachol 46-55 epidermal growth factor receptor Homo sapiens 141-153 10586949-12 1999 ADM and CGRP inhibited carbachol-induced contraction in a concentration-dependent manner with IC50 values of 10 and 90 nM, respectively. Carbachol 23-32 adrenomedullin Homo sapiens 0-3 10586949-12 1999 ADM and CGRP inhibited carbachol-induced contraction in a concentration-dependent manner with IC50 values of 10 and 90 nM, respectively. Carbachol 23-32 calcitonin related polypeptide alpha Homo sapiens 8-12 10586950-7 1999 RESULTS: In tissues precontracted by carbachol PKC antagonist H7 led to a relaxation of TM (25+/-7.2 versus 100%; n = 8) with no effect on CM. Carbachol 37-46 protein kinase C alpha Bos taurus 47-50 10559227-2 1999 The muscarinic agonist of chloride secretion, carbachol (CCh), also stimulates an antisecretory pathway that involves transactivation of the EGF receptor (EGFR) but does not involve PI3-K. Carbachol 46-55 epidermal growth factor receptor Homo sapiens 155-159 10559227-2 1999 The muscarinic agonist of chloride secretion, carbachol (CCh), also stimulates an antisecretory pathway that involves transactivation of the EGF receptor (EGFR) but does not involve PI3-K. Carbachol 57-60 epidermal growth factor receptor Homo sapiens 141-153 10559227-2 1999 The muscarinic agonist of chloride secretion, carbachol (CCh), also stimulates an antisecretory pathway that involves transactivation of the EGF receptor (EGFR) but does not involve PI3-K. Carbachol 57-60 epidermal growth factor receptor Homo sapiens 155-159 10559227-3 1999 Here, we have examined if ErbB receptors, other than the EGFR, have a role in regulation of colonic secretion and if differential effects on ErbB receptor activation may explain the ability of the EGFR to propagate diverse signaling pathways in response to EGF versus CCh. Carbachol 268-271 epidermal growth factor receptor Homo sapiens 197-201 10559227-4 1999 Basolateral, but not apical, addition of the ErbB3/ErbB4 ligand alpha-heregulin (HRG; 1-100 ng/ml) inhibited secretory responses to CCh (100 microM) across voltage-clamped T(84) epithelial cells. Carbachol 132-135 erb-b2 receptor tyrosine kinase 3 Homo sapiens 45-50 10559227-4 1999 Basolateral, but not apical, addition of the ErbB3/ErbB4 ligand alpha-heregulin (HRG; 1-100 ng/ml) inhibited secretory responses to CCh (100 microM) across voltage-clamped T(84) epithelial cells. Carbachol 132-135 erb-b2 receptor tyrosine kinase 4 Homo sapiens 51-56 10559227-7 1999 Further studies revealed that, while both EGF (100 ng/ml) and CCh (100 microM) stimulated phosphorylation of the EGFR, only EGF stimulated phosphorylation of ErbB2, and neither stimulated ErbB3 phosphorylation. Carbachol 62-65 epidermal growth factor receptor Homo sapiens 113-117 10559227-7 1999 Further studies revealed that, while both EGF (100 ng/ml) and CCh (100 microM) stimulated phosphorylation of the EGFR, only EGF stimulated phosphorylation of ErbB2, and neither stimulated ErbB3 phosphorylation. Carbachol 62-65 epidermal growth factor Homo sapiens 113-116 10559227-7 1999 Further studies revealed that, while both EGF (100 ng/ml) and CCh (100 microM) stimulated phosphorylation of the EGFR, only EGF stimulated phosphorylation of ErbB2, and neither stimulated ErbB3 phosphorylation. Carbachol 62-65 erb-b2 receptor tyrosine kinase 3 Homo sapiens 188-193 10559227-10 1999 Differential dimerization with other ErbB family members may underlie the ability of the EGFR to propagate diverse inhibitory signals in response to activation by EGF or transactivation by CCh. Carbachol 189-192 epidermal growth factor receptor Homo sapiens 37-41 10559227-10 1999 Differential dimerization with other ErbB family members may underlie the ability of the EGFR to propagate diverse inhibitory signals in response to activation by EGF or transactivation by CCh. Carbachol 189-192 epidermal growth factor receptor Homo sapiens 89-93 10559227-10 1999 Differential dimerization with other ErbB family members may underlie the ability of the EGFR to propagate diverse inhibitory signals in response to activation by EGF or transactivation by CCh. Carbachol 189-192 epidermal growth factor Homo sapiens 89-92 10514440-4 1999 Inhibition of protein kinase C with Ro31-8220, GF109203x, or Go6976 or down-regulation of protein kinase C inhibited increases in RGS2 mRNA levels induced by carbachol or by the activation of protein kinase C. Blockade of calcium signaling did not alter carbachol-induced increases in RGS2 mRNA levels. Carbachol 158-167 regulator of G protein signaling 2 Homo sapiens 130-134 10561809-8 1999 Aortas also differed by a reduced ability to relax in response to carbamylcholine in hypertensive rats; this effect is hypertension (P < 0.05) and group (P < 0.005) dependent, without any change in carbamylcholine pD2 values. Carbachol 66-81 neurogenin 3 Rattus norvegicus 45-50 10561809-8 1999 Aortas also differed by a reduced ability to relax in response to carbamylcholine in hypertensive rats; this effect is hypertension (P < 0.05) and group (P < 0.005) dependent, without any change in carbamylcholine pD2 values. Carbachol 204-219 neurogenin 3 Rattus norvegicus 45-50 10585535-1 1999 In this study we investigated effects of acute and chronic ethanol exposure on carbachol-induced activator protein-1 (AP-1) DNA binding in rat cerebellar granule cells. Carbachol 79-88 Jun proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 97-116 10585535-1 1999 In this study we investigated effects of acute and chronic ethanol exposure on carbachol-induced activator protein-1 (AP-1) DNA binding in rat cerebellar granule cells. Carbachol 79-88 Jun proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 118-122 10585535-2 1999 Acute ethanol application did not alter, whereas chronic ethanol exposure potentiated the carbachol-induced AP-1 DNA binding. Carbachol 90-99 Jun proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 108-112 10585535-3 1999 The protein composition of the AP-1 transcription factor complex activated by carbachol stimulation of muscarinic receptors was analysed in control and chronic ethanol-exposed cells using a supershift assay with specific antibodies against c-Fos, Fos B, c-Jun, Jun B and Jun D proteins. Carbachol 78-87 Jun proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 31-35 10585535-4 1999 Supershift analysis revealed that the carbachol-induced AP-1 complex was composed predominantly of Jun D and c-Fos. Carbachol 38-47 Jun proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 56-60 10585535-4 1999 Supershift analysis revealed that the carbachol-induced AP-1 complex was composed predominantly of Jun D and c-Fos. Carbachol 38-47 JunD proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 99-104 10585535-4 1999 Supershift analysis revealed that the carbachol-induced AP-1 complex was composed predominantly of Jun D and c-Fos. Carbachol 38-47 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 109-114 10585535-5 1999 The composition of the AP-1 complex activated by carbachol in chronic ethanol-exposed cells did not differ from control. Carbachol 49-58 Jun proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 23-27 10585535-6 1999 These findings indicate that chronic ethanol treatment can modulate carbachol-induced AP-1 DNA binding activity in cerebellar granule cells. Carbachol 68-77 Jun proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 86-90 10514440-2 1999 In human neuroblastoma SH-SY5Y cells stimulation of muscarinic receptors by carbachol activates phosphoinositide signaling and also caused a rapid, large, and long lasting increase in RGS2 mRNA levels. Carbachol 76-85 regulator of G protein signaling 2 Homo sapiens 184-188 10506152-4 1999 The lack of NHE1, but not NHE2 or NHE3, prevented intracellular pH recovery from an acid load in resting acinar cells, in acini stimulated to secrete with the muscarinic agonist carbachol, and in acini shrunken by hypertonic addition of sucrose. Carbachol 178-187 solute carrier family 9 (sodium/hydrogen exchanger), member 1 Mus musculus 12-16 10571577-0 1999 Effect of carbachol on regulation of the mACh receptor mRNA expression ADN insulin secretion in mouse pancreatic islets. Carbachol 10-19 complement factor D (adipsin) Mus musculus 71-74 10519505-2 1999 METHODS: Protein tyrosine phosphorylation and ERK activation induced by carbachol, an agonist for muscarinic acetylcholine receptors, were examined in rat pheochromocytoma PC12 cells, a model for investigating signal transduction. Carbachol 72-81 Eph receptor B1 Rattus norvegicus 46-49 10519505-3 1999 Carbachol-induced tyrosine-phosphorylated proteins of 44 and 42 kd were determined by Western blot analysis and identified as activated ERK1 and ERK2 using anti-ERK antibody. Carbachol 0-9 mitogen activated protein kinase 3 Rattus norvegicus 136-140 10519505-3 1999 Carbachol-induced tyrosine-phosphorylated proteins of 44 and 42 kd were determined by Western blot analysis and identified as activated ERK1 and ERK2 using anti-ERK antibody. Carbachol 0-9 mitogen activated protein kinase 1 Rattus norvegicus 145-149 10519505-3 1999 Carbachol-induced tyrosine-phosphorylated proteins of 44 and 42 kd were determined by Western blot analysis and identified as activated ERK1 and ERK2 using anti-ERK antibody. Carbachol 0-9 Eph receptor B1 Rattus norvegicus 136-139 10519505-6 1999 The effects of three Na+ current-modifying reagents on carbachol-induced ERK activation were also evaluated. Carbachol 55-64 Eph receptor B1 Rattus norvegicus 73-76 10519505-10 1999 The inhibition of carbachol-induced ERK activation by procaine was not modified by a phosphatase inhibitor, calyculin A. Carbachol 18-27 Eph receptor B1 Rattus norvegicus 36-39 10493909-2 1999 Ca(2+) imaging was used to show caffeine-, carbachol- and thapsigargin-induced Ca(2+) release in HEK-293 cells transfected with ryanodine receptor (RyR) cDNA, but only carbachol- and thapsigargin-induced Ca(2+) release in untransfected HEK-293 cells. Carbachol 43-52 ryanodine receptor 1 Homo sapiens 128-146 10493909-2 1999 Ca(2+) imaging was used to show caffeine-, carbachol- and thapsigargin-induced Ca(2+) release in HEK-293 cells transfected with ryanodine receptor (RyR) cDNA, but only carbachol- and thapsigargin-induced Ca(2+) release in untransfected HEK-293 cells. Carbachol 43-52 ryanodine receptor 1 Homo sapiens 148-151 10493909-2 1999 Ca(2+) imaging was used to show caffeine-, carbachol- and thapsigargin-induced Ca(2+) release in HEK-293 cells transfected with ryanodine receptor (RyR) cDNA, but only carbachol- and thapsigargin-induced Ca(2+) release in untransfected HEK-293 cells. Carbachol 168-177 ryanodine receptor 1 Homo sapiens 148-151 10529472-0 1999 Modulation of carbachol-stimulated AP-1 DNA binding activity by therapeutic agents for bipolar disorder in human neuroblastoma SH-SY5Y cells. Carbachol 14-23 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 35-39 10529472-3 1999 AP-1 activation stimulated by carbachol was reduced by pretreatment for 1 h, 24 h or 7 days with 1 mM lithium by 15%, 37%, and 60%, respectively, and with 0.05 mM carbamazepine by 3%, 21%, and 46%, respectively, but not by pretreatment with 0.5 mM sodium valproate. Carbachol 30-39 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 0-4 10529472-4 1999 AP-1 DNA binding activity stimulated by carbachol or by phorbol ester-induced activation of protein kinase C was inhibited by the protein kinase C inhibitor Ro31-8220, but phorbol ester-stimulated AP-1 activation was unaltered by 7-day pretreatments with lithium or carbamazepine. Carbachol 40-49 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 0-4 10529472-5 1999 Activation of AP-1 by carbachol was dependent on calcium, as it was inhibited by treatment with the extracellular calcium chelator EGTA, the intracellular calcium chelator BAPTA-AM, and the calcium/calmodulin kinase II inhibitor KN62. Carbachol 22-31 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 14-18 10529472-7 1999 Thus, chronic treatment with the antibipolar agents lithium and carbamazepine attenuates carbachol-stimulated AP-1 DNA binding activity, and these agents preferentially inhibit signaling cascades activated by the calcium rather than the protein kinase C arm of the phosphoinositide signaling pathway. Carbachol 89-98 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 110-114 10568524-0 1999 Induction of tyrosine hydroxylase and neuropeptide Y by carbachol: modulation with age. Carbachol 56-65 neuropeptide Y Rattus norvegicus 38-52 10501181-1 1999 Activation of muscarinic receptors in human neuroblastoma SH-SY5Y cells with carbachol stimulated a rapid and large increase in early growth response-1 (Egr-1, also called zif268 and NGF1-A) protein levels and DNA binding activity. Carbachol 77-86 early growth response 1 Homo sapiens 128-151 10501181-1 1999 Activation of muscarinic receptors in human neuroblastoma SH-SY5Y cells with carbachol stimulated a rapid and large increase in early growth response-1 (Egr-1, also called zif268 and NGF1-A) protein levels and DNA binding activity. Carbachol 77-86 early growth response 1 Homo sapiens 153-158 10501181-1 1999 Activation of muscarinic receptors in human neuroblastoma SH-SY5Y cells with carbachol stimulated a rapid and large increase in early growth response-1 (Egr-1, also called zif268 and NGF1-A) protein levels and DNA binding activity. Carbachol 77-86 early growth response 1 Homo sapiens 172-178 10501181-2 1999 Egr-1 DNA binding activity was stimulated within 15 min of treatment with carbachol and maintained a maximum 20-fold increase over basal between 1 and 2 h after treatment, and the EC50 was approximately 1 microM carbachol. Carbachol 74-83 early growth response 1 Homo sapiens 0-5 10501181-2 1999 Egr-1 DNA binding activity was stimulated within 15 min of treatment with carbachol and maintained a maximum 20-fold increase over basal between 1 and 2 h after treatment, and the EC50 was approximately 1 microM carbachol. Carbachol 212-221 early growth response 1 Homo sapiens 0-5 10501181-3 1999 Carbachol-stimulated Egr-1 DNA binding activity was dependent on protein kinase C, as it was potently inhibited by GF109203X (IC50 approximately 0.1 microM) and was reduced by 85 +/- 5% by down-regulation of protein kinase C. Inhibitors of increases in intracellular calcium levels reduced carbachol-induced Egr-1 DNA binding activity by 25-35%. Carbachol 0-9 early growth response 1 Homo sapiens 21-26 10501181-3 1999 Carbachol-stimulated Egr-1 DNA binding activity was dependent on protein kinase C, as it was potently inhibited by GF109203X (IC50 approximately 0.1 microM) and was reduced by 85 +/- 5% by down-regulation of protein kinase C. Inhibitors of increases in intracellular calcium levels reduced carbachol-induced Egr-1 DNA binding activity by 25-35%. Carbachol 0-9 early growth response 1 Homo sapiens 308-313 10501181-3 1999 Carbachol-stimulated Egr-1 DNA binding activity was dependent on protein kinase C, as it was potently inhibited by GF109203X (IC50 approximately 0.1 microM) and was reduced by 85 +/- 5% by down-regulation of protein kinase C. Inhibitors of increases in intracellular calcium levels reduced carbachol-induced Egr-1 DNA binding activity by 25-35%. Carbachol 290-299 early growth response 1 Homo sapiens 21-26 10501181-4 1999 Carbachol-stimulated activation of Egr-1 was reduced 35% by genistein, a tyrosine kinase inhibitor, and 60% by PD098059, an inhibitor of mitogen-activated protein kinase kinases 1/2 (MEK1/2) that activates extracellular-regulated kinases 1/2 (ERK1/2). Carbachol 0-9 early growth response 1 Homo sapiens 35-40 10501181-4 1999 Carbachol-stimulated activation of Egr-1 was reduced 35% by genistein, a tyrosine kinase inhibitor, and 60% by PD098059, an inhibitor of mitogen-activated protein kinase kinases 1/2 (MEK1/2) that activates extracellular-regulated kinases 1/2 (ERK1/2). Carbachol 0-9 mitogen-activated protein kinase kinase 1 Homo sapiens 137-181 10501181-4 1999 Carbachol-stimulated activation of Egr-1 was reduced 35% by genistein, a tyrosine kinase inhibitor, and 60% by PD098059, an inhibitor of mitogen-activated protein kinase kinases 1/2 (MEK1/2) that activates extracellular-regulated kinases 1/2 (ERK1/2). Carbachol 0-9 mitogen-activated protein kinase kinase 1 Homo sapiens 183-189 10501181-4 1999 Carbachol-stimulated activation of Egr-1 was reduced 35% by genistein, a tyrosine kinase inhibitor, and 60% by PD098059, an inhibitor of mitogen-activated protein kinase kinases 1/2 (MEK1/2) that activates extracellular-regulated kinases 1/2 (ERK1/2). Carbachol 0-9 mitogen-activated protein kinase 3 Homo sapiens 243-249 10501181-6 1999 Valproate treatment reduced carbachol-stimulated Egr-1 DNA binding activity by 60% but did not alter carbachol-induced activation of ERK1/2 or p38 or increases in Egr-1 protein levels. Carbachol 28-37 early growth response 1 Homo sapiens 49-54 10501181-7 1999 These results reveal that muscarinic receptors activate Egr-1 through a signaling cascade primarily encompassing protein kinase C, MEK1/2, and ERK1/2 and that valproate substantially inhibits Egr-1 DNA binding activity stimulated by carbachol or protein kinase C. Carbachol 233-242 early growth response 1 Homo sapiens 56-61 10501181-7 1999 These results reveal that muscarinic receptors activate Egr-1 through a signaling cascade primarily encompassing protein kinase C, MEK1/2, and ERK1/2 and that valproate substantially inhibits Egr-1 DNA binding activity stimulated by carbachol or protein kinase C. Carbachol 233-242 early growth response 1 Homo sapiens 192-197 10514440-4 1999 Inhibition of protein kinase C with Ro31-8220, GF109203x, or Go6976 or down-regulation of protein kinase C inhibited increases in RGS2 mRNA levels induced by carbachol or by the activation of protein kinase C. Blockade of calcium signaling did not alter carbachol-induced increases in RGS2 mRNA levels. Carbachol 254-263 regulator of G protein signaling 2 Homo sapiens 130-134 10461917-4 1999 Overexpression of G protein-coupled receptor kinase (GRK) 2 caused an increase in the rate constant for receptor endocytosis (from 0.06 to 0.18 min(-1)) and a decrease in the EC50 for carbachol stimulation of internalization (from 15 to 3 microM). Carbachol 184-193 G protein-coupled receptor kinase 2 Mus musculus 18-59 10484474-3 1999 Carbachol-, lysophosphatidic acid (LPA)-, and endothelin-1-induced increases in filamentous actin staining are indicative of actin reorganization (filamentous-to-globular actin ratios of 2.4 +/- 0.3 in control cells, 6.7 +/- 0.8 with carbachol, 7.2 +/- 0.8 with LPA, and 7.4 +/- 0.9 with endothelin-1; P < 0.001; n = 14 experiments). Carbachol 0-9 endothelin 1 Homo sapiens 288-300 10484474-3 1999 Carbachol-, lysophosphatidic acid (LPA)-, and endothelin-1-induced increases in filamentous actin staining are indicative of actin reorganization (filamentous-to-globular actin ratios of 2.4 +/- 0.3 in control cells, 6.7 +/- 0.8 with carbachol, 7.2 +/- 0.8 with LPA, and 7.4 +/- 0.9 with endothelin-1; P < 0.001; n = 14 experiments). Carbachol 234-243 endothelin 1 Homo sapiens 46-58 10484474-5 1999 Although carbachol-induced actin reorganization was blocked in cells pretreated with antisense oligonucleotides directed against Galphai-2 alone, LPA- and endothelin-1-induced actin reorganization were only blocked when both Galphai-2 and G(q)alpha were depleted. Carbachol 9-18 endothelin 1 Homo sapiens 155-167 10519135-9 1999 In human colonocytes (T84) exposed to hSK4 antisense probes, but not to sense probes, carbachol-induced K+ currents were attenuated. Carbachol 86-95 potassium calcium-activated channel subfamily N member 4 Homo sapiens 38-42 10404113-1 1999 We used immunocytochemistry to determine the regional and temporal distribution of Fos protein expression in awake and unrestrained rats after a unilateral stereotaxic microinjection of a cholinergic agonist, carbachol, in the thalamic ventroposterolateral and reticular nuclei, previously shown to cause limbic and generalized convulsive seizures. Carbachol 209-218 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 83-86 10404113-2 1999 The microinjection of carbachol elicits behavioral alterations including immobilization, staring, facial and jaw clonus, rearing, and falling, followed by recurrent generalized convulsive seizures, and a pattern of c-fos expression throughout the brain. Carbachol 22-31 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 215-220 10404113-3 1999 In addition to the hypothalamic paraventricular and supraoptic nuclei, the initial induction of c-fos expression was observed as early as 15 minutes after the carbachol microinjection, in the piriform and entorhinal cortices, the thalamic paraventricular, the supramammilary, the lateral parabrachial nuclei, and the central gray. Carbachol 159-168 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 96-101 10441519-6 1999 Carbachol also induced degradation of IkappaBalpha, which was reversed by addition of both GF109203X and PDTC and stimulated the activity of a NF-kappaB-luciferase reporter gene plasmid in COS-7 cells stably expressing the human M3 muscarinic receptor. Carbachol 0-9 cholinergic receptor muscarinic 3 Homo sapiens 229-251 10423442-7 1999 After treatment with carbachol, which stimulates contraction and PKC activity, in addition to the membrane localization, the activated PKC exhibited a pronounced cytosolic fibrillar distribution and an increased total fluorescence intensity relative to vinculin. Carbachol 21-30 proline rich transmembrane protein 2 Homo sapiens 65-68 10423442-7 1999 After treatment with carbachol, which stimulates contraction and PKC activity, in addition to the membrane localization, the activated PKC exhibited a pronounced cytosolic fibrillar distribution and an increased total fluorescence intensity relative to vinculin. Carbachol 21-30 proline rich transmembrane protein 2 Homo sapiens 135-138 10423442-7 1999 After treatment with carbachol, which stimulates contraction and PKC activity, in addition to the membrane localization, the activated PKC exhibited a pronounced cytosolic fibrillar distribution and an increased total fluorescence intensity relative to vinculin. Carbachol 21-30 vinculin Homo sapiens 253-261 10461917-5 1999 Overexpression of a dominant negative form of GRK2 had more modest effects, reducing the rate constant for endocytosis (from 0.11 to 0.07 min(-1)) and increasing the EC50 for carbachol stimulation of internalization (from 8 to 17 microM). Carbachol 175-184 G protein-coupled receptor kinase 2 Mus musculus 46-50 10362652-13 1999 We then investigated the role of betagamma-subunits in carbachol induction of HA-ERK2. Carbachol 55-64 mitogen-activated protein kinase 1 Canis lupus familiaris 78-85 10419481-8 1999 Investigating the cell cycle mechanisms involved in growth inhibition, we found that carbachol treatment decreased cyclin D1 levels, increased p21(cip1) expression, and led to hypophosphorylation of the retinoblastoma gene product (Rb). Carbachol 85-94 cyclin D1 Mus musculus 115-124 10448929-0 1999 Involvement of calmodulin and protein kinase C in the regulation of K+ transport by carbachol across the rat distal colon. Carbachol 84-93 calmodulin 1 Rattus norvegicus 15-25 10448929-4 1999 The Ca2+-calmodulin antagonist calmidazolium (10(-7) mol l(-1)) inhibited the stimulation of mucosal and serosal Rb+ efflux by carbachol. Carbachol 127-136 calmodulin 1 Rattus norvegicus 9-19 10448929-8 1999 Both calmidazolium and staurosporine, but not KN-62, prevented the stimulatory action of carbachol on the H+-K+-ATPase, suggesting a synergistic control of this ion pump by both Ca2+-calmodulin and protein kinase C. Carbachol 89-98 calmodulin 1 Rattus norvegicus 183-193 10409227-5 1999 With the use of intact human bronchial rings, alpha-thrombin (1-20 U/ml) increased bronchial tone to 19 +/- 3% of basal tone (P = 0.008; n = 5 experiments) and represents 20 +/- 8% of the maximum carbachol response. Carbachol 196-205 coagulation factor II, thrombin Homo sapiens 52-60 10419481-8 1999 Investigating the cell cycle mechanisms involved in growth inhibition, we found that carbachol treatment decreased cyclin D1 levels, increased p21(cip1) expression, and led to hypophosphorylation of the retinoblastoma gene product (Rb). Carbachol 85-94 cyclin-dependent kinase inhibitor 1A (P21) Mus musculus 143-146 10419481-8 1999 Investigating the cell cycle mechanisms involved in growth inhibition, we found that carbachol treatment decreased cyclin D1 levels, increased p21(cip1) expression, and led to hypophosphorylation of the retinoblastoma gene product (Rb). Carbachol 85-94 cyclin-dependent kinase inhibitor 1A (P21) Mus musculus 147-151 10419481-9 1999 Proteasome inhibitors blocked the carbachol-induced degradation of cyclin D1. Carbachol 34-43 cyclin D1 Mus musculus 67-76 10362652-0 1999 Carbachol activates ERK2 in isolated gastric parietal cells via multiple signaling pathways. Carbachol 0-9 mitogen-activated protein kinase 1 Canis lupus familiaris 20-24 10362652-1 1999 We previously reported that both carbachol and epidermal growth factor (EGF) are potent inducers of the extracellular signal-regulated protein kinases (ERKs) in isolated gastric canine parietal cells and that induction of these kinases leads to acute inhibitory and chronic stimulatory effects on gastric acid secretion. Carbachol 33-42 mitogen-activated protein kinase 1 Canis lupus familiaris 152-156 10362652-6 1999 Dominant negative Ras reduced carbachol induction of HA-ERK2 activity by 60% and completely inhibited the stimulatory effect of EGF. Carbachol 30-39 mitogen-activated protein kinase 1 Canis lupus familiaris 53-60 10362652-15 1999 In the presence of this vector, carbachol induction of HA-ERK2 was inhibited by 40%. Carbachol 32-41 mitogen-activated protein kinase 1 Canis lupus familiaris 55-62 10362652-16 1999 Together these data suggest that, in the gastric parietal cells, carbachol activates the ERKs through Ras- and betagamma-dependent mechanisms that require guanine nucleotide exchange factors other than Sos. Carbachol 65-74 mitogen-activated protein kinase 1 Canis lupus familiaris 89-93 10417666-4 1999 Carbachol (CCh, 10 micromol/l ) induced a 335+/-10 nmol/l (n=8) rise in [Ca2+ ]i in control myotubes and 531.9+/-32 nmol/l (n=23) in LGMD2C myotubes. Carbachol 0-9 sarcoglycan gamma Homo sapiens 133-139 10417666-4 1999 Carbachol (CCh, 10 micromol/l ) induced a 335+/-10 nmol/l (n=8) rise in [Ca2+ ]i in control myotubes and 531.9+/-32 nmol/l (n=23) in LGMD2C myotubes. Carbachol 11-14 sarcoglycan gamma Homo sapiens 133-139 10234035-1 1999 Although it has long been known that microinjection of the cholinergic agonist carbachol into the medial pontine reticular formation (mPRF) induces a state that resembles rapid eye movement (REM) sleep, it is likely that other transmitters contribute to mPRF regulation of behavioral states. Carbachol 79-88 Spi-C transcription factor (Spi-1/PU.1 related) Mus musculus 134-138 10234035-1 1999 Although it has long been known that microinjection of the cholinergic agonist carbachol into the medial pontine reticular formation (mPRF) induces a state that resembles rapid eye movement (REM) sleep, it is likely that other transmitters contribute to mPRF regulation of behavioral states. Carbachol 79-88 Spi-C transcription factor (Spi-1/PU.1 related) Mus musculus 254-258 10331408-4 1999 Adenovirus-mediated overexpression of PLC-delta1, -beta1, or -beta3 in INS-1 or betaG 40/110 cells results in little or no enhancement in inositol phosphate (IP) accumulation and no improvement in insulin secretion when the cells are stimulated with glucose or carbachol, despite the fact that the overexpressed proteins are fully active in cell extracts. Carbachol 261-270 phospholipase C, delta 1 Rattus norvegicus 38-67 10579054-4 1999 Application of the cholinergic agonist carbamylcholine (100 microM) to transfected S2-DM1 cells expressing a Drosophila muscarinic acetylcholine receptor (DM1) emptied the InsP3-sensitive Ca2+ store but failed to affect the amplitude of alkalinization-evoked Ca2+ release. Carbachol 39-54 muscarinic Acetylcholine Receptor, A-type Drosophila melanogaster 86-89 10579054-4 1999 Application of the cholinergic agonist carbamylcholine (100 microM) to transfected S2-DM1 cells expressing a Drosophila muscarinic acetylcholine receptor (DM1) emptied the InsP3-sensitive Ca2+ store but failed to affect the amplitude of alkalinization-evoked Ca2+ release. Carbachol 39-54 muscarinic Acetylcholine Receptor, A-type Drosophila melanogaster 120-153 10579054-4 1999 Application of the cholinergic agonist carbamylcholine (100 microM) to transfected S2-DM1 cells expressing a Drosophila muscarinic acetylcholine receptor (DM1) emptied the InsP3-sensitive Ca2+ store but failed to affect the amplitude of alkalinization-evoked Ca2+ release. Carbachol 39-54 muscarinic Acetylcholine Receptor, A-type Drosophila melanogaster 155-158 10579054-4 1999 Application of the cholinergic agonist carbamylcholine (100 microM) to transfected S2-DM1 cells expressing a Drosophila muscarinic acetylcholine receptor (DM1) emptied the InsP3-sensitive Ca2+ store but failed to affect the amplitude of alkalinization-evoked Ca2+ release. Carbachol 39-54 Inositol 1,4,5,-trisphosphate receptor Drosophila melanogaster 172-177 10436402-3 1999 To study the role of NO in mediating the effect of carbachol on Na-HCO(3) cotransporter, we measured the activity of the cotransporter in rabbit proximal tubule cells treated with carbachol (10(-4 )M) or the NO inhibitor, L-NAME (10(-3) M), or carbachol+L-NAME. Carbachol 51-60 electrogenic sodium bicarbonate cotransporter 1 Oryctolagus cuniculus 64-87 10215681-4 1999 SLPI also inhibited the development of airway hyperresponsiveness to carbachol (84%, p <. Carbachol 69-78 antileukoproteinase Ovis aries 0-4 10352035-20 1999 We conclude that the differences between CCh- and CCK-induced calcium oscillations in pancreatic acinar cells can be explained by two principal mechanisms: (a) CCK causes more phosphorylation of the IP3 receptor than does CCh, and the phosphorylated receptor cannot pass calcium current; and (b) the rate of calcium ATPase pumping and the rate of calcium influx from the outside the cell are greater in the presence of CCh than in the presence of CCK. Carbachol 41-44 cholecystokinin Homo sapiens 160-163 10352035-20 1999 We conclude that the differences between CCh- and CCK-induced calcium oscillations in pancreatic acinar cells can be explained by two principal mechanisms: (a) CCK causes more phosphorylation of the IP3 receptor than does CCh, and the phosphorylated receptor cannot pass calcium current; and (b) the rate of calcium ATPase pumping and the rate of calcium influx from the outside the cell are greater in the presence of CCh than in the presence of CCK. Carbachol 41-44 inositol 1,4,5-trisphosphate receptor type 3 Homo sapiens 199-211 10352035-20 1999 We conclude that the differences between CCh- and CCK-induced calcium oscillations in pancreatic acinar cells can be explained by two principal mechanisms: (a) CCK causes more phosphorylation of the IP3 receptor than does CCh, and the phosphorylated receptor cannot pass calcium current; and (b) the rate of calcium ATPase pumping and the rate of calcium influx from the outside the cell are greater in the presence of CCh than in the presence of CCK. Carbachol 41-44 cholecystokinin Homo sapiens 160-163 10352035-20 1999 We conclude that the differences between CCh- and CCK-induced calcium oscillations in pancreatic acinar cells can be explained by two principal mechanisms: (a) CCK causes more phosphorylation of the IP3 receptor than does CCh, and the phosphorylated receptor cannot pass calcium current; and (b) the rate of calcium ATPase pumping and the rate of calcium influx from the outside the cell are greater in the presence of CCh than in the presence of CCK. Carbachol 222-225 cholecystokinin Homo sapiens 50-53 10352035-20 1999 We conclude that the differences between CCh- and CCK-induced calcium oscillations in pancreatic acinar cells can be explained by two principal mechanisms: (a) CCK causes more phosphorylation of the IP3 receptor than does CCh, and the phosphorylated receptor cannot pass calcium current; and (b) the rate of calcium ATPase pumping and the rate of calcium influx from the outside the cell are greater in the presence of CCh than in the presence of CCK. Carbachol 222-225 cholecystokinin Homo sapiens 50-53 10200428-5 1999 Recombinant prenylated Rnd1 (0.01-0.1 mg ml-1) dose dependently inhibited carbachol- and GTPgammaS-induced Ca2+ sensitization in beta-escin-permeabilized ileal smooth muscle strips but had no effect on the tension at submaximal [Ca2+] (pCa 6.3). Carbachol 74-83 Rho family GTPase 1 Rattus norvegicus 23-27 10213914-8 1999 The cholinergic agonist carbachol (CAR) (10(-7) M) also released CRH and this action was blocked by ATR (10(-7) M). Carbachol 24-33 nuclear receptor subfamily 1, group I, member 3 Rattus norvegicus 35-38 10213914-8 1999 The cholinergic agonist carbachol (CAR) (10(-7) M) also released CRH and this action was blocked by ATR (10(-7) M). Carbachol 24-33 corticotropin releasing hormone Rattus norvegicus 65-68 10213914-8 1999 The cholinergic agonist carbachol (CAR) (10(-7) M) also released CRH and this action was blocked by ATR (10(-7) M). Carbachol 24-33 ATR serine/threonine kinase Rattus norvegicus 100-103 10411313-1 1999 Pituitary adenylyl cyclase activating polypeptide (PACAP-27), forskoline and carbachol increased type A atrial natriuretic peptide receptor (NPR-A) density, as well as NPR-A mRNA level, in the human neuroblastoma NB-OK-1 cell line. Carbachol 77-86 natriuretic peptide receptor 1 Homo sapiens 141-146 10411313-1 1999 Pituitary adenylyl cyclase activating polypeptide (PACAP-27), forskoline and carbachol increased type A atrial natriuretic peptide receptor (NPR-A) density, as well as NPR-A mRNA level, in the human neuroblastoma NB-OK-1 cell line. Carbachol 77-86 natriuretic peptide receptor 1 Homo sapiens 168-173 10411313-4 1999 Our data support an original transcriptional upregulation of human NPR-A in response to cAMP-induced agents, and in response to carbachol. Carbachol 128-137 natriuretic peptide receptor 1 Homo sapiens 67-72 10101239-0 1999 Hippocampal neurotrophin and trk receptor mRNA levels are altered by local administration of nicotine, carbachol and pilocarpine. Carbachol 103-112 brain derived neurotrophic factor Homo sapiens 12-24 10436402-5 1999 In control cells, carbachol significantly increased Na-HCO(3) cotransporter activity while L-NAME did not affect the activity of the cotransporter but completely blocked the enhancement induced by carbachol. Carbachol 18-27 solute carrier family 4 member 4 Homo sapiens 52-75 10101239-8 1999 Nicotine and carbachol caused transient decreases in NT-3 mRNA levels in dentate gyrus and CA2 with pilocarpine showing a similar trend. Carbachol 13-22 carbonic anhydrase 2 Homo sapiens 91-94 10187833-5 1999 In these studies we confirmed that H7, but not HA1004, potently blocks the induction of zif268 and c-fos mRNA by nerve growth factor, carbachol, phorbol ester, Ca2+ ionophore, or forskolin. Carbachol 134-143 early growth response 1 Rattus norvegicus 88-94 10187833-5 1999 In these studies we confirmed that H7, but not HA1004, potently blocks the induction of zif268 and c-fos mRNA by nerve growth factor, carbachol, phorbol ester, Ca2+ ionophore, or forskolin. Carbachol 134-143 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 99-104 10101239-0 1999 Hippocampal neurotrophin and trk receptor mRNA levels are altered by local administration of nicotine, carbachol and pilocarpine. Carbachol 103-112 neurotrophic receptor tyrosine kinase 1 Homo sapiens 29-32 10101239-6 1999 In contrast, carbachol and pilocarpine produced a transient increase in NGF mRNA levels present 4-8 h after drug administration. Carbachol 13-22 nerve growth factor Homo sapiens 72-75 9989779-8 1999 Moreover, the muscarinic agonist carbachol was found to inhibit MELC differentiation by decreasing by approximately 35% the amount of benzidine-positive (B+) cells in HMBA-induced cultures and, to a lesser degree, also AChE levels. Carbachol 33-42 acetylcholinesterase Mus musculus 219-223 10209246-5 1999 To test the hypothesis that activation of mAchR also increases phosphorylation of B-50, protein phosphorylation has been examined in cerebral cortical slices in response to the cholinergic agonist, carbachol (Cch) in comparison to the phorbol ester, 4beta-phorbol 12, 13-dibutyrate (PDB), a known activator of PKC. Carbachol 198-207 growth associated protein 43 Homo sapiens 82-86 10209246-6 1999 At short times of incubation with 1 mM Cch, a concentration which maximally activates PI metabolism, increased phosphorylation of a group of synaptosomal proteins, including B-50 and myristoylated, alanine-rich C kinase substrate (MARCKS), was observed. Carbachol 39-42 growth associated protein 43 Homo sapiens 174-229 10209246-6 1999 At short times of incubation with 1 mM Cch, a concentration which maximally activates PI metabolism, increased phosphorylation of a group of synaptosomal proteins, including B-50 and myristoylated, alanine-rich C kinase substrate (MARCKS), was observed. Carbachol 39-42 myristoylated alanine rich protein kinase C substrate Homo sapiens 231-237 10209246-9 1999 Phosphorylation of B-50 and MARCKS was sensitive to Cch in both cases. Carbachol 52-55 growth associated protein 43 Homo sapiens 19-23 10209246-9 1999 Phosphorylation of B-50 and MARCKS was sensitive to Cch in both cases. Carbachol 52-55 myristoylated alanine rich protein kinase C substrate Homo sapiens 28-34 10198350-3 1999 Secretin also elicited relaxation of carbachol-stimulated rat forestomach muscle strips by binding to its receptors, suggesting a direct action on this peripheral tissue. Carbachol 37-46 secretin Rattus norvegicus 0-8 10323594-6 1999 In binding assays none of the compounds studied displayed preferential affinity for the M1,3,4 or M5 subtypes although carbachol was less potent at hM1 than hM3,4,5. Carbachol 119-128 cholinergic receptor muscarinic 1 Homo sapiens 148-151 10103113-3 1999 We report that in rat hippocampal neuronal cultures, GP120 induces a dramatic and persistent increase in [Ca2+]i which is prevented by drugs that either deplete (caffeine, carbachol, thapsigargin) or block (dantrolene) Ca2+ release from intracellular stores. Carbachol 172-181 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 53-58 10103124-5 1999 Responses induced by NMDA, carbachol or both agonists on microdiscs were reduced by phospholipase A2 inhibitors, the most striking effects being observed with mepacrine. Carbachol 27-36 phospholipase A2, group IB, pancreas Mus musculus 84-100 10203138-6 1999 However, Cftr-/- pancreatic acini showed a significantly greater amylase response to the combination of BrcAMP and carbachol than the sum of the individual responses in separate experiments (p < 0.05). Carbachol 115-124 cystic fibrosis transmembrane conductance regulator Mus musculus 9-13 10203138-10 1999 However, Cftr-/- pancreatic acini exhibited a synergistic secretory response following stimulation by BrcAMP plus carbachol. Carbachol 114-123 cystic fibrosis transmembrane conductance regulator Mus musculus 9-13 10070131-4 1999 Jejunal segments from anti-CD3-treated mice displayed a significantly elevated epithelial baseline short-circuit current (which indicates increased ion transport) and a concomitant reduction in responsiveness to prosecretory stimuli (nerve stimulation, carbachol, and forskolin). Carbachol 253-262 CD3 antigen, epsilon polypeptide Mus musculus 27-30 10198335-4 1999 CCK (10 pM-10 nM) and carbachol (0.1-100 microM) dose dependently increased the amount of immunodetectable RhoA with a peak increase occurring at 3 min. Carbachol 22-31 ras homolog family member A Rattus norvegicus 107-111 10198335-6 1999 Although an increase in RhoA did not require the presence of extracellular Ca2+, the intracellular Ca2+ chelator 1, 2-bis(2-aminophenoxy)ethane-N,N,N",N"-tetraacetic acid-AM abolished the appearance of the RhoA band in response to CCK and carbachol. Carbachol 239-248 ras homolog family member A Rattus norvegicus 24-28 10198335-13 1999 We concluded that the small GTP-binding protein RhoA p21 exists in pancreatic acini and appears to be involved in the mediation of pancreatic enzyme secretion evoked by CCK and carbachol. Carbachol 177-186 ras homolog family member A Rattus norvegicus 48-52 10198335-13 1999 We concluded that the small GTP-binding protein RhoA p21 exists in pancreatic acini and appears to be involved in the mediation of pancreatic enzyme secretion evoked by CCK and carbachol. Carbachol 177-186 KRAS proto-oncogene, GTPase Rattus norvegicus 53-56 10198354-4 1999 Carbachol and endothelin-1 increased GTP-bound p21(ras) in a pertussis toxin-sensitive manner [ratio of [32P]GTP to ([32P]GTP + [32P]GDP): control, 30 +/- 1.7; 3 min of 1 microM carbachol, 39 +/- 1.1; 3 min of 1 microM endothelin-1, 40 +/- 1.2], whereas histamine, bradykinin, and KCl were without effect. Carbachol 0-9 H3 histone pseudogene 16 Homo sapiens 47-50 10198354-4 1999 Carbachol and endothelin-1 increased GTP-bound p21(ras) in a pertussis toxin-sensitive manner [ratio of [32P]GTP to ([32P]GTP + [32P]GDP): control, 30 +/- 1.7; 3 min of 1 microM carbachol, 39 +/- 1.1; 3 min of 1 microM endothelin-1, 40 +/- 1.2], whereas histamine, bradykinin, and KCl were without effect. Carbachol 0-9 endothelin 1 Homo sapiens 219-231 10198354-4 1999 Carbachol and endothelin-1 increased GTP-bound p21(ras) in a pertussis toxin-sensitive manner [ratio of [32P]GTP to ([32P]GTP + [32P]GDP): control, 30 +/- 1.7; 3 min of 1 microM carbachol, 39 +/- 1.1; 3 min of 1 microM endothelin-1, 40 +/- 1.2], whereas histamine, bradykinin, and KCl were without effect. Carbachol 0-9 kininogen 1 Homo sapiens 265-275 10198354-4 1999 Carbachol and endothelin-1 increased GTP-bound p21(ras) in a pertussis toxin-sensitive manner [ratio of [32P]GTP to ([32P]GTP + [32P]GDP): control, 30 +/- 1.7; 3 min of 1 microM carbachol, 39 +/- 1.1; 3 min of 1 microM endothelin-1, 40 +/- 1.2], whereas histamine, bradykinin, and KCl were without effect. Carbachol 178-187 endothelin 1 Homo sapiens 14-26 10198354-4 1999 Carbachol and endothelin-1 increased GTP-bound p21(ras) in a pertussis toxin-sensitive manner [ratio of [32P]GTP to ([32P]GTP + [32P]GDP): control, 30 +/- 1.7; 3 min of 1 microM carbachol, 39 +/- 1.1; 3 min of 1 microM endothelin-1, 40 +/- 1.2], whereas histamine, bradykinin, and KCl were without effect. Carbachol 178-187 H3 histone pseudogene 16 Homo sapiens 47-50 10203138-4 1999 Carbachol and BrcAMP or BrcAMP and forskolin, given in combination, produced additive effects on enzyme secretion in the Cftr+/+ acini. Carbachol 0-9 cystic fibrosis transmembrane conductance regulator Mus musculus 121-125 10066893-7 1999 Carbachol (CCh, 1-30 microM) increased the inwardly rectifying current in 70 % of GIRK1+ GIRK2-transfected cells by 261 +/- 53 % (n = 6, CCh 30 microM) at -120 mV, but had no effect in non-transfected cells or in cells transfected with GIRK1 or GIRK2 alone. Carbachol 0-9 potassium inwardly-rectifying channel, subfamily J, member 3 Rattus norvegicus 82-87 10066893-7 1999 Carbachol (CCh, 1-30 microM) increased the inwardly rectifying current in 70 % of GIRK1+ GIRK2-transfected cells by 261 +/- 53 % (n = 6, CCh 30 microM) at -120 mV, but had no effect in non-transfected cells or in cells transfected with GIRK1 or GIRK2 alone. Carbachol 0-9 potassium inwardly-rectifying channel, subfamily J, member 6 Rattus norvegicus 89-94 10066893-7 1999 Carbachol (CCh, 1-30 microM) increased the inwardly rectifying current in 70 % of GIRK1+ GIRK2-transfected cells by 261 +/- 53 % (n = 6, CCh 30 microM) at -120 mV, but had no effect in non-transfected cells or in cells transfected with GIRK1 or GIRK2 alone. Carbachol 0-9 potassium inwardly-rectifying channel, subfamily J, member 3 Rattus norvegicus 236-241 10066893-7 1999 Carbachol (CCh, 1-30 microM) increased the inwardly rectifying current in 70 % of GIRK1+ GIRK2-transfected cells by 261 +/- 53 % (n = 6, CCh 30 microM) at -120 mV, but had no effect in non-transfected cells or in cells transfected with GIRK1 or GIRK2 alone. Carbachol 0-9 potassium inwardly-rectifying channel, subfamily J, member 6 Rattus norvegicus 245-250 10066893-7 1999 Carbachol (CCh, 1-30 microM) increased the inwardly rectifying current in 70 % of GIRK1+ GIRK2-transfected cells by 261 +/- 53 % (n = 6, CCh 30 microM) at -120 mV, but had no effect in non-transfected cells or in cells transfected with GIRK1 or GIRK2 alone. Carbachol 11-14 potassium inwardly-rectifying channel, subfamily J, member 3 Rattus norvegicus 82-87 10066893-7 1999 Carbachol (CCh, 1-30 microM) increased the inwardly rectifying current in 70 % of GIRK1+ GIRK2-transfected cells by 261 +/- 53 % (n = 6, CCh 30 microM) at -120 mV, but had no effect in non-transfected cells or in cells transfected with GIRK1 or GIRK2 alone. Carbachol 11-14 potassium inwardly-rectifying channel, subfamily J, member 6 Rattus norvegicus 89-94 10066893-7 1999 Carbachol (CCh, 1-30 microM) increased the inwardly rectifying current in 70 % of GIRK1+ GIRK2-transfected cells by 261 +/- 53 % (n = 6, CCh 30 microM) at -120 mV, but had no effect in non-transfected cells or in cells transfected with GIRK1 or GIRK2 alone. Carbachol 11-14 potassium inwardly-rectifying channel, subfamily J, member 3 Rattus norvegicus 236-241 10066893-7 1999 Carbachol (CCh, 1-30 microM) increased the inwardly rectifying current in 70 % of GIRK1+ GIRK2-transfected cells by 261 +/- 53 % (n = 6, CCh 30 microM) at -120 mV, but had no effect in non-transfected cells or in cells transfected with GIRK1 or GIRK2 alone. Carbachol 11-14 potassium inwardly-rectifying channel, subfamily J, member 6 Rattus norvegicus 245-250 10070103-5 1999 TNF-alpha treatment for 72 h significantly increased the expression of Galphai-2 and Gqalpha proteins and enhanced carbachol (10(-7) M)-mediated inhibition of adenylyl cyclase activity and inositol phosphate synthesis. Carbachol 115-124 tumor necrosis factor Homo sapiens 0-9 9989779-9 1999 The carbachol effect on erythroid differentiation was reverted by atropine that was found to restore the original amount of B+ cells, while it reduced acetylcholinesterase (AChE) to levels of approximately 66% of control. Carbachol 4-13 acetylcholinesterase Mus musculus 151-171 9989779-9 1999 The carbachol effect on erythroid differentiation was reverted by atropine that was found to restore the original amount of B+ cells, while it reduced acetylcholinesterase (AChE) to levels of approximately 66% of control. Carbachol 4-13 acetylcholinesterase Mus musculus 173-177 10208302-7 1999 Linear Schild plots with slopes near to unity indicated that [F/G]NC(1-13)NH2 is a competitive antagonist, specific for NC receptors both in vitro (since it was inactive on opioid receptors) and in vivo (since it was inactive against carbachol). Carbachol 234-243 prepronociceptin Rattus norvegicus 66-68 10049786-3 1999 Activation of muscarinic receptors with carbachol was found to inhibit EGF-induced signaling, including tyrosine phosphorylation of the adaptor protein Cbl and of the EGF receptor, and complex formation between Shc proteins and the EGF receptor and Grb2. Carbachol 40-49 epidermal growth factor Homo sapiens 71-74 10049786-3 1999 Activation of muscarinic receptors with carbachol was found to inhibit EGF-induced signaling, including tyrosine phosphorylation of the adaptor protein Cbl and of the EGF receptor, and complex formation between Shc proteins and the EGF receptor and Grb2. Carbachol 40-49 Cbl proto-oncogene Homo sapiens 152-155 10049786-3 1999 Activation of muscarinic receptors with carbachol was found to inhibit EGF-induced signaling, including tyrosine phosphorylation of the adaptor protein Cbl and of the EGF receptor, and complex formation between Shc proteins and the EGF receptor and Grb2. Carbachol 40-49 epidermal growth factor receptor Homo sapiens 167-179 10049786-3 1999 Activation of muscarinic receptors with carbachol was found to inhibit EGF-induced signaling, including tyrosine phosphorylation of the adaptor protein Cbl and of the EGF receptor, and complex formation between Shc proteins and the EGF receptor and Grb2. Carbachol 40-49 epidermal growth factor receptor Homo sapiens 232-244 10049786-3 1999 Activation of muscarinic receptors with carbachol was found to inhibit EGF-induced signaling, including tyrosine phosphorylation of the adaptor protein Cbl and of the EGF receptor, and complex formation between Shc proteins and the EGF receptor and Grb2. Carbachol 40-49 growth factor receptor bound protein 2 Homo sapiens 249-253 10208302-7 1999 Linear Schild plots with slopes near to unity indicated that [F/G]NC(1-13)NH2 is a competitive antagonist, specific for NC receptors both in vitro (since it was inactive on opioid receptors) and in vivo (since it was inactive against carbachol). Carbachol 234-243 prepronociceptin Rattus norvegicus 120-122 9920901-6 1999 The response of mouse pancreatic acini to carbachol was about 4- and 33-fold more sensitive to RGS4 than that of bombesin and cholecystokinin (CCK), respectively. Carbachol 42-51 regulator of G-protein signaling 4 Mus musculus 95-99 10081931-3 1999 These Ca2+-responses were augmented by +70% by focally applied carbachol. Carbachol 63-72 carbonic anhydrase 2 Rattus norvegicus 6-9 10048785-3 1999 Thus, ANP (1 microM) increased cGMP production in the SV-CISM-2 cells and CISM cells by 487- and 1.7-fold, respectively, and inhibited CCh-induced [Ca2+]i mobilisation by 95 and 3%, respectively. Carbachol 135-138 natriuretic peptide A Homo sapiens 6-9 10048785-4 1999 In the SV-CISM-2 cells, ANP and CNP dose dependently inhibited CCh-induced [Ca2+]i mobilisation with IC50 values of 156 and 412 nM, respectively, and dose dependently stimulated cGMP formation with EC50 values of 24 and 88 nM, respectively, suggesting that the inhibitory actions of the peptides are mediated through cGMP. Carbachol 63-66 natriuretic peptide A Homo sapiens 24-27 10048785-4 1999 In the SV-CISM-2 cells, ANP and CNP dose dependently inhibited CCh-induced [Ca2+]i mobilisation with IC50 values of 156 and 412 nM, respectively, and dose dependently stimulated cGMP formation with EC50 values of 24 and 88 nM, respectively, suggesting that the inhibitory actions of the peptides are mediated through cGMP. Carbachol 63-66 natriuretic peptide C Homo sapiens 32-35 10348664-5 1999 That carbachol can activate src family kinases was indicated further by the finding that carbachol induced an increase in tyrosine phosphorylation of p120-src substrate, which was inhibited by PP1. Carbachol 5-14 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 28-31 10348664-5 1999 That carbachol can activate src family kinases was indicated further by the finding that carbachol induced an increase in tyrosine phosphorylation of p120-src substrate, which was inhibited by PP1. Carbachol 5-14 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 155-158 10348664-5 1999 That carbachol can activate src family kinases was indicated further by the finding that carbachol induced an increase in tyrosine phosphorylation of p120-src substrate, which was inhibited by PP1. Carbachol 5-14 neuropeptide Y receptor Y4 Homo sapiens 193-196 10348664-5 1999 That carbachol can activate src family kinases was indicated further by the finding that carbachol induced an increase in tyrosine phosphorylation of p120-src substrate, which was inhibited by PP1. Carbachol 89-98 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 28-31 10348664-5 1999 That carbachol can activate src family kinases was indicated further by the finding that carbachol induced an increase in tyrosine phosphorylation of p120-src substrate, which was inhibited by PP1. Carbachol 89-98 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 155-158 10348664-5 1999 That carbachol can activate src family kinases was indicated further by the finding that carbachol induced an increase in tyrosine phosphorylation of p120-src substrate, which was inhibited by PP1. Carbachol 89-98 neuropeptide Y receptor Y4 Homo sapiens 193-196 10348664-8 1999 In contrast to the results with carbachol, H2O2 potentiated EGF-induced tyrosine phosphorylation. Carbachol 32-41 epidermal growth factor Homo sapiens 60-63 10050013-2 1999 We have investigated, with a combined in vitro and in vivo approach, the influence on insulin and glucagon release stimulated by the cholinergic, muscarinic agonist carbachol of different NO modulators, i.e. the nitric oxide synthase (NOS) inhibitors NG-nitro-L-arginine methyl ester (L-NAME), NG-monomethyl-L-arginine (L-NMMA) and 7-nitroindazole as well as the intracellular NO donor hydroxylamine. Carbachol 165-174 insulin Homo sapiens 86-93 10050013-4 1999 At basal glucose (7 mM) carbachol dose-dependently stimulated insulin release from isolated islets with a half-maximal response at approximately 1 microM of the agonist. Carbachol 24-33 insulin Homo sapiens 62-69 10050013-7 1999 Carbachol-stimulated islets displayed an increased insulin release and a suppressed glucagon release in the presence of L-NAME, L-NMMA or 7-nitroindazole. Carbachol 0-9 insulin Homo sapiens 51-58 10050013-9 1999 The intracellular NO donor hydroxylamine dose-dependently inhibited carbachol-induced insulin release but stimulated glucagon release only at a low concentration (3 microM). Carbachol 68-77 insulin Homo sapiens 86-93 10050013-11 1999 In islets depolarized with 30 mM K+ in the presence of the KATP channel opener diazoxide, NOS inhibition by 5 mM L-NAME still markedly potentiated carbachol-induced insulin release (although less so than in normal islets) and suppressed glucagon release. Carbachol 147-156 insulin Homo sapiens 165-172 10082202-2 1999 ATP, carbachol and thapsigargin increased endothelial [Ca2+]i in rabbit aortic valve loaded with a leakage resistant, fluorescent Ca2+ indicator, fura-PE3. Carbachol 5-14 carbonic anhydrase 2 Oryctolagus cuniculus 55-58 10082202-2 1999 ATP, carbachol and thapsigargin increased endothelial [Ca2+]i in rabbit aortic valve loaded with a leakage resistant, fluorescent Ca2+ indicator, fura-PE3. Carbachol 5-14 carbonic anhydrase 2 Oryctolagus cuniculus 130-133 10082202-7 1999 When the increase in endothelial [Ca2+]i was plotted against the relaxation, the carbachol-induced increase in [Ca2+]i elicited greater relaxation than did ATP or thapsigargin at a given [Ca2+]i. Carbachol 81-90 carbonic anhydrase 2 Oryctolagus cuniculus 34-37 10082202-7 1999 When the increase in endothelial [Ca2+]i was plotted against the relaxation, the carbachol-induced increase in [Ca2+]i elicited greater relaxation than did ATP or thapsigargin at a given [Ca2+]i. Carbachol 81-90 carbonic anhydrase 2 Oryctolagus cuniculus 112-115 10082202-7 1999 When the increase in endothelial [Ca2+]i was plotted against the relaxation, the carbachol-induced increase in [Ca2+]i elicited greater relaxation than did ATP or thapsigargin at a given [Ca2+]i. Carbachol 81-90 carbonic anhydrase 2 Oryctolagus cuniculus 112-115 9915989-2 1999 We previously demonstrated that TNF-alpha induces a small, delayed follicular stage-dependent increase in intracellular Ca2+ concentration ([Ca2+]i) in hen granulosa cells and promotes carbachol (Cch)-induced mobilization of Ca2+ from intracellular stores in cells otherwise unresponsive to the cytokine. Carbachol 185-194 tumor necrosis factor Homo sapiens 32-41 9915989-2 1999 We previously demonstrated that TNF-alpha induces a small, delayed follicular stage-dependent increase in intracellular Ca2+ concentration ([Ca2+]i) in hen granulosa cells and promotes carbachol (Cch)-induced mobilization of Ca2+ from intracellular stores in cells otherwise unresponsive to the cytokine. Carbachol 196-199 tumor necrosis factor Homo sapiens 32-41 9925822-8 1999 Moreover, carbachol stimulated GFP-MK2 translocation to the cytoplasm in the absence of anisomycin. Carbachol 10-19 MAPK activated protein kinase 2 Homo sapiens 35-38 10348664-3 1999 Carbachol induced time-dependent increases in phosphotyrosine immunoreactivity of several protein bands, which were quantitated, and immunoprecipitation was used to identify the adhesion-related proteins focal adhesion kinase, p130Cas/HEF1, and paxillin, and three shc adapter proteins. Carbachol 0-9 BCAR1 scaffold protein, Cas family member Homo sapiens 227-234 10348664-3 1999 Carbachol induced time-dependent increases in phosphotyrosine immunoreactivity of several protein bands, which were quantitated, and immunoprecipitation was used to identify the adhesion-related proteins focal adhesion kinase, p130Cas/HEF1, and paxillin, and three shc adapter proteins. Carbachol 0-9 neural precursor cell expressed, developmentally down-regulated 9 Homo sapiens 235-239 10348664-4 1999 Carbachol-induced tyrosine phosphorylation of the adhesion-related proteins was mediated by muscarinic receptors, and was inhibited by a src family kinase inhibitor, PP1. Carbachol 0-9 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 137-140 10348664-4 1999 Carbachol-induced tyrosine phosphorylation of the adhesion-related proteins was mediated by muscarinic receptors, and was inhibited by a src family kinase inhibitor, PP1. Carbachol 0-9 neuropeptide Y receptor Y4 Homo sapiens 166-169 9882625-1 1999 The signalling pathway leading to an activation of mitogen-activated protein (MAP) kinase subtypes Erk-1 and -2 upon stimulation of muscarinic receptor with carbachol in human neuroblastoma SK-N-BE2(C) cells was investigated. Carbachol 157-166 mitogen-activated protein kinase 3 Homo sapiens 99-111 9882625-2 1999 Carbachol activated Erk-1/-2 by stimulating M3 muscarinic receptor, as determined by specific antagonists for individual muscarinic receptors. Carbachol 0-9 mitogen-activated protein kinase 3 Homo sapiens 20-28 9882625-2 1999 Carbachol activated Erk-1/-2 by stimulating M3 muscarinic receptor, as determined by specific antagonists for individual muscarinic receptors. Carbachol 0-9 cholinergic receptor muscarinic 3 Homo sapiens 44-66 9882625-3 1999 The activation of Erk-1/-2 by carbachol was blocked by the inhibition or down-regulation of protein kinase C (PKC). Carbachol 30-39 mitogen-activated protein kinase 3 Homo sapiens 18-26 9882625-3 1999 The activation of Erk-1/-2 by carbachol was blocked by the inhibition or down-regulation of protein kinase C (PKC). Carbachol 30-39 protein kinase C epsilon Homo sapiens 110-113 9882625-4 1999 Among the multiple PKC isoforms expressed in SK-N-BE2(C) cells, only PKCepsilon was activated by the treatment of carbachol, and selective down-regulation of PKCepsilon was sufficient to block Erk-1/-2 activation. Carbachol 114-123 protein kinase C epsilon Homo sapiens 19-22 9882625-4 1999 Among the multiple PKC isoforms expressed in SK-N-BE2(C) cells, only PKCepsilon was activated by the treatment of carbachol, and selective down-regulation of PKCepsilon was sufficient to block Erk-1/-2 activation. Carbachol 114-123 protein kinase C epsilon Homo sapiens 69-79 9882625-5 1999 Carbachol treatment induced activation of the serine/threonine protein kinase Raf, and an inhibition of Raf blocked Erk-1/-2 activation. Carbachol 0-9 zinc fingers and homeoboxes 2 Homo sapiens 78-81 9882625-5 1999 Carbachol treatment induced activation of the serine/threonine protein kinase Raf, and an inhibition of Raf blocked Erk-1/-2 activation. Carbachol 0-9 zinc fingers and homeoboxes 2 Homo sapiens 104-107 9882625-5 1999 Carbachol treatment induced activation of the serine/threonine protein kinase Raf, and an inhibition of Raf blocked Erk-1/-2 activation. Carbachol 0-9 mitogen-activated protein kinase 3 Homo sapiens 116-124 9882625-6 1999 Ectopic expression of inhibitory small GTPase Ras, RasN17, blocked the carbachol-induced Raf activation without affecting the activation of PKCepsilon, while the inhibition of PKC blocked the Raf activation. Carbachol 71-80 zinc fingers and homeoboxes 2 Homo sapiens 89-92 9882625-7 1999 Thus, these results suggest that carbachol-induced activation of PKCepsilon mediates Erk-1/-2 activation by a sequential activation of Ras, Raf and MAP kinase kinase. Carbachol 33-42 protein kinase C epsilon Homo sapiens 65-75 9882625-7 1999 Thus, these results suggest that carbachol-induced activation of PKCepsilon mediates Erk-1/-2 activation by a sequential activation of Ras, Raf and MAP kinase kinase. Carbachol 33-42 mitogen-activated protein kinase 3 Homo sapiens 85-93 9882625-7 1999 Thus, these results suggest that carbachol-induced activation of PKCepsilon mediates Erk-1/-2 activation by a sequential activation of Ras, Raf and MAP kinase kinase. Carbachol 33-42 zinc fingers and homeoboxes 2 Homo sapiens 140-143 9920901-8 1999 RGS1 showed approximately 1000-fold higher potency in inhibiting carbachol than CCK-dependent signaling. Carbachol 65-74 regulator of G-protein signaling 1 Mus musculus 0-4 9920901-9 1999 RGS16 was as effective as RGS1 in inhibiting carbachol-dependent signaling but only partially inhibited the response to CCK. Carbachol 45-54 regulator of G-protein signaling 16 Mus musculus 0-5 9920901-9 1999 RGS16 was as effective as RGS1 in inhibiting carbachol-dependent signaling but only partially inhibited the response to CCK. Carbachol 45-54 regulator of G-protein signaling 1 Mus musculus 0-4 9920901-10 1999 By contrast, RGS2 inhibited the response to carbachol and CCK with equal potency. Carbachol 44-53 regulator of G-protein signaling 2 Mus musculus 13-17 10226762-7 1999 BRL 37344A, a selective beta 3-adrenoceptor agonist produced concentration-dependent relaxation of carbachol-precontracted fundic and ductal strips. Carbachol 99-108 beta-3 adrenergic receptor Ovis aries 24-43 9873004-4 1999 In cells expressing homotetrameric wild type or mutant RyR1, the amplitude of 10 mM caffeine-induced Ca2+ release was correlated significantly with the amplitude of carbachol- or thapsigargin-induced Ca2+ release, indicating that maximal drug-induced Ca2+ release depends on the size of the endoplasmic reticulum Ca2+ store. Carbachol 165-174 ryanodine receptor 1 Homo sapiens 55-59 9872844-8 1999 CGRP (10(-)6 M) reduced by more than 75% the extent of the contraction evoked by 10(-)6 M of carbamylcholine and its protector effect was totally abolished in bronchi showing clear morphological manifestation of inflammatory reaction. Carbachol 93-108 calcitonin-related polypeptide alpha Rattus norvegicus 0-4 9886064-5 1999 The results also showed that CREB phosphorylation in immature OLG precursor cells could be up-regulated by treatment with histamine, carbachol, glutamate, and ATP (neuroligands known to increase Ca2+ levels in these cells), by signaling cascade(s) that involve a protein kinase C activity, as well as the mitogen-activated protein kinase pathway. Carbachol 133-142 cAMP responsive element binding protein 1 Rattus norvegicus 29-33 9874691-5 1999 In control cells expressing alpha1B, alpha2, and beta3 Ca channel subunits and m2 receptors, carbachol (1 microM) inhibited whole-cell currents by approximately 80% compared with only approximately 55% inhibition in cells also expressing exogenous RGS protein. Carbachol 93-102 eukaryotic translation elongation factor 1 beta 2 pseudogene 2 Homo sapiens 37-54 9874691-5 1999 In control cells expressing alpha1B, alpha2, and beta3 Ca channel subunits and m2 receptors, carbachol (1 microM) inhibited whole-cell currents by approximately 80% compared with only approximately 55% inhibition in cells also expressing exogenous RGS protein. Carbachol 93-102 paired like homeodomain 2 Homo sapiens 248-251 9973241-3 1999 Two NO donors inhibited activation of neuronal NO synthase (nNOS) in response to the muscarinic receptor agonist carbachol in Chinese hamster ovary (CHO) cells stably transfected with the M1 muscarinic receptor and nNOS. Carbachol 113-122 nitric oxide synthase 1 Homo sapiens 60-64 9973241-3 1999 Two NO donors inhibited activation of neuronal NO synthase (nNOS) in response to the muscarinic receptor agonist carbachol in Chinese hamster ovary (CHO) cells stably transfected with the M1 muscarinic receptor and nNOS. Carbachol 113-122 nitric oxide synthase 1 Homo sapiens 215-219 9973241-11 1999 A decrease in the carbachol-mediated transient Ca2+ peak was observed in cells that express nNOS as compared to cells lacking the enzyme, suggesting that endogenous NO might inhibit receptor mediated Ca2+ signaling. Carbachol 18-27 nitric oxide synthase 1 Homo sapiens 92-96 9815052-0 1998 Supramaximal CCK and CCh concentrations abolish VIP potentiation by inhibiting adenylyl cyclase activity. Carbachol 21-24 vasoactive intestinal peptide Rattus norvegicus 48-51 10193897-4 1999 The inhibition of the carbachol (1 mM) response by CRH was concentration-dependent (EC50 = 154 +/- 1.8 nM). Carbachol 22-31 corticotropin releasing hormone Mus musculus 51-54 10193897-5 1999 Calcium responses to sub-maximally effective concentrations of PACAP (5 nM), VIP (400 nM) and carbachol (1 mM) were abolished by prior exposure to CRH (1 microM). Carbachol 94-103 corticotropin releasing hormone Mus musculus 147-150 10049140-1 1998 The difference between infrared spectra of the nicotinic acetylcholine receptor (nAChR) recorded in the absence and presence of the agonist carbamylcholine (Carb) reveals a complex pattern of positive and negative bands that provides a spectral map of Carb-induced structural change. Carbachol 140-155 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 47-79 10049140-1 1998 The difference between infrared spectra of the nicotinic acetylcholine receptor (nAChR) recorded in the absence and presence of the agonist carbamylcholine (Carb) reveals a complex pattern of positive and negative bands that provides a spectral map of Carb-induced structural change. Carbachol 140-155 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 81-86 10049140-1 1998 The difference between infrared spectra of the nicotinic acetylcholine receptor (nAChR) recorded in the absence and presence of the agonist carbamylcholine (Carb) reveals a complex pattern of positive and negative bands that provides a spectral map of Carb-induced structural change. Carbachol 157-161 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 47-79 10049140-1 1998 The difference between infrared spectra of the nicotinic acetylcholine receptor (nAChR) recorded in the absence and presence of the agonist carbamylcholine (Carb) reveals a complex pattern of positive and negative bands that provides a spectral map of Carb-induced structural change. Carbachol 157-161 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 81-86 10049140-1 1998 The difference between infrared spectra of the nicotinic acetylcholine receptor (nAChR) recorded in the absence and presence of the agonist carbamylcholine (Carb) reveals a complex pattern of positive and negative bands that provides a spectral map of Carb-induced structural change. Carbachol 252-256 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 47-79 10049140-1 1998 The difference between infrared spectra of the nicotinic acetylcholine receptor (nAChR) recorded in the absence and presence of the agonist carbamylcholine (Carb) reveals a complex pattern of positive and negative bands that provides a spectral map of Carb-induced structural change. Carbachol 252-256 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 81-86 10049140-4 1998 Spectral variations are also observed that are indicative of both the displacement of the anesthetics from the nAChR upon the addition of Carb and physical interactions that occur between the anesthetics and binding site residues. Carbachol 138-142 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 111-116 9802318-3 1998 Relaxation by CGRP (1 microM) was determined in cells maximally contracted by carbachol (CCh, 1 nM). Carbachol 78-87 calcitonin related polypeptide alpha Homo sapiens 14-18 9802318-3 1998 Relaxation by CGRP (1 microM) was determined in cells maximally contracted by carbachol (CCh, 1 nM). Carbachol 89-92 calcitonin related polypeptide alpha Homo sapiens 14-18 9802318-5 1998 CCh-induced contraction was inhibited by 1 microM CGRP (maximum: 69+/-5% within 60 sec); similarly, exposure of cells to sodium nitroprussiate (SNP), 1 microM, fully inhibited contraction (maximum: 89+/-8% within 30 sec). Carbachol 0-3 calcitonin related polypeptide alpha Homo sapiens 50-54 9863995-4 1998 In tracheal strips precontracted with carbachol, hypocapnic challenge (0% CO2) produced increases in tension, pHi, and [Ca2+]i. Carbachol 38-47 glucose-6-phosphate isomerase Homo sapiens 110-113 9853304-0 1998 Potentiation by neurotensin of carbachol-induced tension development in beta-escin-skinned smooth muscle of guinea-pig ileum. Carbachol 31-40 neurotensin/neuromedin N Cavia porcellus 16-27 9853304-1 1998 Effect of neurotensin (NT) on carbachol(CCh)-induced tension development due to Ca2+ release from intracellular stores was investigated in beta-escin-skinned smooth muscle of guinea-pig ileum. Carbachol 30-39 neurotensin/neuromedin N Cavia porcellus 10-21 9853304-1 1998 Effect of neurotensin (NT) on carbachol(CCh)-induced tension development due to Ca2+ release from intracellular stores was investigated in beta-escin-skinned smooth muscle of guinea-pig ileum. Carbachol 30-39 neurotensin/neuromedin N Cavia porcellus 23-25 11286343-4 1998 The mPRF was microinjected with 0.25 ml saline, carbachol (4.0 microg), neostigmine (6.7 microg), or morphine sulfate (14.7 microg), and TFL measures were obtained in response to radiant heat. Carbachol 48-57 Spi-C transcription factor (Spi-1/PU.1 related) Mus musculus 4-8 11286343-5 1998 During wakefulness TFL (% increase) was not increased by morphine or saline, but was significantly increased by mPRF administration of carbachol (42.4%) and neostigmine (35.2%). Carbachol 135-144 Spi-C transcription factor (Spi-1/PU.1 related) Mus musculus 112-116 9786868-4 1998 Chronic exposure of cells expressing alpha3 beta2 AChRs or alpha3 beta2 alpha5 AChRs to nicotine or carbamylcholine increased their amount up to 24-fold but had no effect on the amount of alpha3beta4 or alpha3 beta4 alpha5 AChRs, i.e. the up-regulation of alpha3 AChRs depended on the presence of beta2 but not beta4 subunits in the AChRs. Carbachol 100-115 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 44-49 9786868-4 1998 Chronic exposure of cells expressing alpha3 beta2 AChRs or alpha3 beta2 alpha5 AChRs to nicotine or carbamylcholine increased their amount up to 24-fold but had no effect on the amount of alpha3beta4 or alpha3 beta4 alpha5 AChRs, i.e. the up-regulation of alpha3 AChRs depended on the presence of beta2 but not beta4 subunits in the AChRs. Carbachol 100-115 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 66-71 9786868-4 1998 Chronic exposure of cells expressing alpha3 beta2 AChRs or alpha3 beta2 alpha5 AChRs to nicotine or carbamylcholine increased their amount up to 24-fold but had no effect on the amount of alpha3beta4 or alpha3 beta4 alpha5 AChRs, i.e. the up-regulation of alpha3 AChRs depended on the presence of beta2 but not beta4 subunits in the AChRs. Carbachol 100-115 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 66-71 9765228-0 1998 Carbachol stimulates transactivation of epidermal growth factor receptor and mitogen-activated protein kinase in T84 cells. Carbachol 0-9 epidermal growth factor receptor Homo sapiens 40-72 9765228-5 1998 Further studies revealed that CCh stimulated an increase in both phosphorylation and activity of the extracellular signal-regulated kinase (ERK) isoforms of mitogen-activated protein kinase. Carbachol 30-33 mitogen-activated protein kinase 1 Homo sapiens 101-138 9765228-5 1998 Further studies revealed that CCh stimulated an increase in both phosphorylation and activity of the extracellular signal-regulated kinase (ERK) isoforms of mitogen-activated protein kinase. Carbachol 30-33 mitogen-activated protein kinase 1 Homo sapiens 140-143 9765228-7 1998 Phosphorylation of ERK in response to CCh was mimicked by the protein kinase C (PKC) activator, phorbol myristate acetate (100 nM), but was not altered by the PKC inhibitor GF 109203X (1 microM). Carbachol 38-41 mitogen-activated protein kinase 1 Homo sapiens 19-22 9765228-9 1998 Immunoprecipitation/Western blot studies revealed that CCh stimulated tyrosine phosphorylation of the EGF receptor (EGFr) and increased co-immunoprecipitation of the adapter proteins, Shc and Grb2, with the EGFr. Carbachol 55-58 epidermal growth factor receptor Homo sapiens 102-114 9765228-9 1998 Immunoprecipitation/Western blot studies revealed that CCh stimulated tyrosine phosphorylation of the EGF receptor (EGFr) and increased co-immunoprecipitation of the adapter proteins, Shc and Grb2, with the EGFr. Carbachol 55-58 epidermal growth factor receptor Homo sapiens 116-120 9765228-9 1998 Immunoprecipitation/Western blot studies revealed that CCh stimulated tyrosine phosphorylation of the EGF receptor (EGFr) and increased co-immunoprecipitation of the adapter proteins, Shc and Grb2, with the EGFr. Carbachol 55-58 SHC adaptor protein 1 Homo sapiens 184-187 9765228-9 1998 Immunoprecipitation/Western blot studies revealed that CCh stimulated tyrosine phosphorylation of the EGF receptor (EGFr) and increased co-immunoprecipitation of the adapter proteins, Shc and Grb2, with the EGFr. Carbachol 55-58 growth factor receptor bound protein 2 Homo sapiens 192-196 9765228-9 1998 Immunoprecipitation/Western blot studies revealed that CCh stimulated tyrosine phosphorylation of the EGF receptor (EGFr) and increased co-immunoprecipitation of the adapter proteins, Shc and Grb2, with the EGFr. Carbachol 55-58 epidermal growth factor receptor Homo sapiens 207-211 9765228-10 1998 An inhibitor of EGFr phosphorylation, tyrphostin AG1478 (1 microM), reversed CCh-stimulated phosphorylation of both EGFr and ERK. Carbachol 77-80 epidermal growth factor receptor Homo sapiens 16-20 9765228-10 1998 An inhibitor of EGFr phosphorylation, tyrphostin AG1478 (1 microM), reversed CCh-stimulated phosphorylation of both EGFr and ERK. Carbachol 77-80 epidermal growth factor receptor Homo sapiens 116-120 9765228-10 1998 An inhibitor of EGFr phosphorylation, tyrphostin AG1478 (1 microM), reversed CCh-stimulated phosphorylation of both EGFr and ERK. Carbachol 77-80 mitogen-activated protein kinase 1 Homo sapiens 125-128 9765228-12 1998 We conclude that CCh activates ERK in T84 cells via a mechanism involving transactivation of the EGFr, and that this pathway constitutes an inhibitory signaling pathway by which chloride secretory responses to CCh may be negatively regulated. Carbachol 17-20 mitogen-activated protein kinase 1 Homo sapiens 31-34 9765228-12 1998 We conclude that CCh activates ERK in T84 cells via a mechanism involving transactivation of the EGFr, and that this pathway constitutes an inhibitory signaling pathway by which chloride secretory responses to CCh may be negatively regulated. Carbachol 17-20 epidermal growth factor receptor Homo sapiens 97-101 9809810-6 1998 Carbachol, insulin, and EGF caused a significant increase in TK immunoreactive protein content which was blocked by HAC and DHC. Carbachol 0-9 TXK tyrosine kinase Homo sapiens 61-63 9809810-7 1998 In BLM, carbachol significantly stimulated HCO3-dependent 22Na uptake and this effect was totally prevented by the monoclonal antibody against TK. Carbachol 8-17 TXK tyrosine kinase Homo sapiens 143-145 9809810-8 1998 In cultured proximal tubule cells, carbachol, EGF and insulin at physiologic concentration caused a significant stimulation of the cotransporter activity and this effect was completely blocked by the TK inhibitor, HAC. Carbachol 35-44 TXK tyrosine kinase Homo sapiens 200-202 9756505-10 1998 Carbachol activation of JNK1 resulted in induction of c-Jun (protein) transcriptional activity and in stimulation of parietal cell mRNA content of c-jun. Carbachol 0-9 Jun proto-oncogene, AP-1 transcription factor subunit Canis lupus familiaris 54-59 9756505-10 1998 Carbachol activation of JNK1 resulted in induction of c-Jun (protein) transcriptional activity and in stimulation of parietal cell mRNA content of c-jun. Carbachol 0-9 Jun proto-oncogene, AP-1 transcription factor subunit Canis lupus familiaris 147-152 9756505-11 1998 In conclusion, our data indicate that carbachol induces JNK activity in gastric parietal cells via intracellular Ca2+-dependent, PKC-independent pathways, leading to induction of c-jun gene expression via phosphorylation and transcriptional activation of c-Jun. Carbachol 38-47 Jun proto-oncogene, AP-1 transcription factor subunit Canis lupus familiaris 179-184 9756505-11 1998 In conclusion, our data indicate that carbachol induces JNK activity in gastric parietal cells via intracellular Ca2+-dependent, PKC-independent pathways, leading to induction of c-jun gene expression via phosphorylation and transcriptional activation of c-Jun. Carbachol 38-47 Jun proto-oncogene, AP-1 transcription factor subunit Canis lupus familiaris 255-260 9751489-5 1998 In tests of several neurotransmitters, only the cholinergic agonists carbachol and bethanechol stimulated peptide secretion in a dose-dependent fashion (by 2.3 +/- 0.5- and 1.7 +/- 0.3-fold at 1000 microM; P < 0.05-0.001); the beta-adrenergic agonist isoproterenol and the chloride channel inhibitor gamma-aminobutyric acid did not affect release of GLP-1. Carbachol 69-78 glucagon Mus musculus 353-358 9756635-1 1998 Desensitization of human muscarinic acetylcholine receptor m2 subtypes (hm2 receptors) stably expressed in chinese hamster ovary cells was measured as decreases in the carbamylcholine-stimulated [35S]GTPgammaS binding activity in membrane preparations after pre-treatment of cells with carbamylcholine. Carbachol 168-183 cholinergic receptor muscarinic 2 Homo sapiens 25-61 9756635-1 1998 Desensitization of human muscarinic acetylcholine receptor m2 subtypes (hm2 receptors) stably expressed in chinese hamster ovary cells was measured as decreases in the carbamylcholine-stimulated [35S]GTPgammaS binding activity in membrane preparations after pre-treatment of cells with carbamylcholine. Carbachol 168-183 cholinergic receptor muscarinic 2 Homo sapiens 72-75 9756635-1 1998 Desensitization of human muscarinic acetylcholine receptor m2 subtypes (hm2 receptors) stably expressed in chinese hamster ovary cells was measured as decreases in the carbamylcholine-stimulated [35S]GTPgammaS binding activity in membrane preparations after pre-treatment of cells with carbamylcholine. Carbachol 286-301 cholinergic receptor muscarinic 2 Homo sapiens 72-75 9756635-2 1998 The extent of carbamylcholine-stimulated [35S]GTPgammaS binding activity was found to decrease to 64% following pretreatment of cells with 10 microM carbamylcholine for 30 min, and under the same conditions 51-59% of hm2 receptors were sequestered/internalized as assessed by decreases in the [3H]N-methylscopolamine binding activity on the cell surface. Carbachol 14-29 cholinergic receptor muscarinic 2 Homo sapiens 217-220 9756635-2 1998 The extent of carbamylcholine-stimulated [35S]GTPgammaS binding activity was found to decrease to 64% following pretreatment of cells with 10 microM carbamylcholine for 30 min, and under the same conditions 51-59% of hm2 receptors were sequestered/internalized as assessed by decreases in the [3H]N-methylscopolamine binding activity on the cell surface. Carbachol 149-164 cholinergic receptor muscarinic 2 Homo sapiens 217-220 9756635-3 1998 A similar reduction in the carbamylcholine-stimulated [35S]GTPgammaS binding activity was observed by pretreatment of cells with 5 nM propylbenzylylcholine mustard, which irreversibly bound to and inactivated 58% of the hm2 receptors. Carbachol 27-42 cholinergic receptor muscarinic 2 Homo sapiens 220-223 9727040-4 1998 The effects of carbachol and bombesin on p38 MAP kinase activity were similar to those of CCK, whereas phorbol ester, epidermal growth factor, and vasoactive intestinal polypeptide stimulated p38 MAP kinase by 2-fold or less. Carbachol 15-24 mitogen activated protein kinase 14 Rattus norvegicus 41-44 10353591-3 1999 In diabetics, the PKC activity in the nucleus fraction was significantly decreased by about 63% in the resting condition and that in the cytosol fraction was significantly increased by about 135% after the treatment with 10 microM CCh for 10 min compared to controls. Carbachol 231-234 protein kinase C, beta Rattus norvegicus 18-21 9875705-5 1998 Protein kinase C (PKC) inhibitors, H-7 and calphostin C, inhibited the carbachol-induced upregulation. Carbachol 71-80 solute carrier family 9 member A2 Rattus norvegicus 35-55 9815039-3 1998 Carbachol (10(-4) M) was the weakest acid secretagogue but caused the strongest Na+/H+ exchange activation, which was completely blocked by 1 microM HOE-642 (selective for NHE1); histamine (10(-4) M) and forskolin (10(-5) M) were stronger stimulants of [14C]aminopyrine accumulation but weaker stimulants of Na+/H+ exchange activity. Carbachol 0-9 sodium/hydrogen exchanger 1 Oryctolagus cuniculus 172-176 9815040-7 1998 However, when parietal cells were preincubated with IL-1beta (0.5-5 pg/ml) for 10 min before the addition of histamine or carbachol, the response to these secretagogues was reduced by 35 and 67%, respectively. Carbachol 122-131 interleukin 1 beta Rattus norvegicus 52-60 9815040-9 1998 Preincubation of parietal cells with IL-1beta failed to alter histamine-stimulated cAMP production but markedly inhibited carbachol-induced formation of D-myo-inositol 1,4, 5-trisphosphate. Carbachol 122-131 interleukin 1 beta Rattus norvegicus 37-45 9815040-10 1998 In fura 2-loaded, purified parietal cells, 10 min preincubation with IL-1beta dramatically reduced the initial transient peak elevation of intracellular Ca2+ concentration in response to carbachol. Carbachol 187-196 interleukin 1 beta Rattus norvegicus 69-77 11717867-2 1998 METHODS: The fura-2 microfluorimetry was used to measure changes of [Ca2+]i in OHCs of the guinea pig cochlea after application of acetylcholine, ATP and carbacholine. Carbachol 154-166 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 69-72 11717867-3 1998 RESULTS: Acetylcholine, ATP and carbacholine increased [Ca2+]i (acetylcholine: 0.74 +/- 0.12 mumol/L, ATP: 0.65 +/- 0.11 mumol/L, carbacholine: 1.16 +/- 0.27 mumol/L) in OHCs in the presence of extracellular Ca2+. Carbachol 32-44 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 56-59 11717867-3 1998 RESULTS: Acetylcholine, ATP and carbacholine increased [Ca2+]i (acetylcholine: 0.74 +/- 0.12 mumol/L, ATP: 0.65 +/- 0.11 mumol/L, carbacholine: 1.16 +/- 0.27 mumol/L) in OHCs in the presence of extracellular Ca2+. Carbachol 32-44 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 208-211 11717867-3 1998 RESULTS: Acetylcholine, ATP and carbacholine increased [Ca2+]i (acetylcholine: 0.74 +/- 0.12 mumol/L, ATP: 0.65 +/- 0.11 mumol/L, carbacholine: 1.16 +/- 0.27 mumol/L) in OHCs in the presence of extracellular Ca2+. Carbachol 130-142 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 56-59 11717867-5 1998 CONCLUSION: Acetylcholine and carbacholine, the cholinergic mascarinic agonists, increased [Ca2+]i in OHCs by acting at receptor-induced ion channel resulting in Ca2+ efflux. Carbachol 30-42 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 92-95 11717867-5 1998 CONCLUSION: Acetylcholine and carbacholine, the cholinergic mascarinic agonists, increased [Ca2+]i in OHCs by acting at receptor-induced ion channel resulting in Ca2+ efflux. Carbachol 30-42 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 162-165 9736657-7 1998 The developmental increase in atrial CNTF receptor mRNA was enhanced by stimulating muscarinic receptors with carbachol in ovo and was inhibited by blocking muscarinic cholinergic receptors with atropine. Carbachol 110-119 ciliary neurotrophic factor Gallus gallus 37-41 9730946-1 1998 In the estrogen-treated rat myometrium, carbachol increased the generation of inositol phosphates by stimulating the muscarinic receptor-Gq/G11-phospholipase C-beta3 (PLC-beta3) cascade. Carbachol 40-49 phospholipase C beta 3 Rattus norvegicus 167-176 9730946-2 1998 Exposure to carbachol resulted in a rapid and specific (homologous) attenuation of the subsequent muscarinic responses in terms of inositol phosphate production, PLC-beta3 translocation to membrane, and contraction. Carbachol 12-21 phospholipase C beta 3 Rattus norvegicus 162-171 9726232-2 1998 In this study, we examined whether CCK-8 stimulates the Ca2+-independent form of PLA2 in isolated rat islets, in comparison with stimulation by the PLA2-activating cholinergic agonist carbachol. Carbachol 184-193 phospholipase A2 group IB Rattus norvegicus 148-152 9732369-4 1998 Choline, a normal component of growth media, showed an efficacy comparable to acetylcholine and carbachol at Hm1(Ser388Tyr, Thr389Pro) receptors. Carbachol 96-105 cholinergic receptor muscarinic 1 Homo sapiens 109-112 9774217-2 1998 Low density lipoprotein (LDL: 20-80 mg/dl) and lipoprotein(a) [Lp(a); 10-80 mg/dl] inhibited catecholamine secretion induced by carbachol, an activator of nicotinic acetylcholine receptor-ion channels. Carbachol 128-137 plasminogen Bos taurus 47-61 9774217-2 1998 Low density lipoprotein (LDL: 20-80 mg/dl) and lipoprotein(a) [Lp(a); 10-80 mg/dl] inhibited catecholamine secretion induced by carbachol, an activator of nicotinic acetylcholine receptor-ion channels. Carbachol 128-137 plasminogen Bos taurus 63-68 9774217-3 1998 LDL and Lp(a) suppressed carbachol-induced 22Na+ influx as well as 45Ca2+ influx in a concentration-dependent manner similar to that of catecholamine secretion. Carbachol 25-34 plasminogen Bos taurus 8-13 9774217-6 1998 Like LDL and Lp(a), a synthetic peptide homologous to human plasma apolipoprotein B (apoB), apoB fragment(3358-3372)-amide (3-60 microM), attenuated 22Na+ influx, 45Ca2+ influx, and catecholamine secretion caused by carbachol. Carbachol 216-225 lipoprotein(a) Homo sapiens 13-18 9774217-6 1998 Like LDL and Lp(a), a synthetic peptide homologous to human plasma apolipoprotein B (apoB), apoB fragment(3358-3372)-amide (3-60 microM), attenuated 22Na+ influx, 45Ca2+ influx, and catecholamine secretion caused by carbachol. Carbachol 216-225 apolipoprotein B Homo sapiens 67-83 9774217-6 1998 Like LDL and Lp(a), a synthetic peptide homologous to human plasma apolipoprotein B (apoB), apoB fragment(3358-3372)-amide (3-60 microM), attenuated 22Na+ influx, 45Ca2+ influx, and catecholamine secretion caused by carbachol. Carbachol 216-225 apolipoprotein B Homo sapiens 85-89 9774217-6 1998 Like LDL and Lp(a), a synthetic peptide homologous to human plasma apolipoprotein B (apoB), apoB fragment(3358-3372)-amide (3-60 microM), attenuated 22Na+ influx, 45Ca2+ influx, and catecholamine secretion caused by carbachol. Carbachol 216-225 apolipoprotein B Homo sapiens 92-96 9815052-1 1998 Exocrine pancreatic secretion stimulated by vasoactive intestinal polypeptide (VIP), which acts through the adenylyl cyclase-cAMP pathway, is potentiated by stimulation with other secretagogues such as CCK and carbachol (CCh). Carbachol 210-219 vasoactive intestinal peptide Rattus norvegicus 44-77 9815052-1 1998 Exocrine pancreatic secretion stimulated by vasoactive intestinal polypeptide (VIP), which acts through the adenylyl cyclase-cAMP pathway, is potentiated by stimulation with other secretagogues such as CCK and carbachol (CCh). Carbachol 210-219 vasoactive intestinal peptide Rattus norvegicus 79-82 9815052-1 1998 Exocrine pancreatic secretion stimulated by vasoactive intestinal polypeptide (VIP), which acts through the adenylyl cyclase-cAMP pathway, is potentiated by stimulation with other secretagogues such as CCK and carbachol (CCh). Carbachol 210-219 cholecystokinin Rattus norvegicus 202-205 9815052-1 1998 Exocrine pancreatic secretion stimulated by vasoactive intestinal polypeptide (VIP), which acts through the adenylyl cyclase-cAMP pathway, is potentiated by stimulation with other secretagogues such as CCK and carbachol (CCh). Carbachol 221-224 vasoactive intestinal peptide Rattus norvegicus 44-77 9815052-1 1998 Exocrine pancreatic secretion stimulated by vasoactive intestinal polypeptide (VIP), which acts through the adenylyl cyclase-cAMP pathway, is potentiated by stimulation with other secretagogues such as CCK and carbachol (CCh). Carbachol 221-224 vasoactive intestinal peptide Rattus norvegicus 79-82 9815052-1 1998 Exocrine pancreatic secretion stimulated by vasoactive intestinal polypeptide (VIP), which acts through the adenylyl cyclase-cAMP pathway, is potentiated by stimulation with other secretagogues such as CCK and carbachol (CCh). Carbachol 221-224 cholecystokinin Rattus norvegicus 202-205 9815052-3 1998 In the present study, we examined the mechanisms by which supramaximal concentrations of CCK octapeptide (CCK-8) or CCh reduce the VIP-induced potentiation of amylase secretion from isolated rat pancreatic acini. Carbachol 116-119 vasoactive intestinal peptide Rattus norvegicus 131-134 9815052-4 1998 VIP-stimulated amylase secretion was potentiated by submaximal stimulatory concentrations of CCK-8 and CCh but was reduced by the same reagents at higher concentrations. Carbachol 103-106 vasoactive intestinal peptide Rattus norvegicus 0-3 9815052-6 1998 Moreover, supramaximal concentrations of CCK-8 or CCh inhibited VIP-stimulated intracellular cAMP production as well as adenylyl cyclase activity. Carbachol 50-53 vasoactive intestinal peptide Rattus norvegicus 64-67 9815052-8 1998 These results indicate that supramaximal concentrations of CCK-8 and CCh reduce the potentiating effect of VIP and forskolin on amylase secretion by inhibiting the adenylyl cyclase activity. Carbachol 69-72 vasoactive intestinal peptide Rattus norvegicus 107-110 9688692-3 1998 Intracerebroventricular infusions of ANG II, 0.75 mol/l NaCl, or carbachol caused increases in renal Na+ and K+ excretion, arterial pressure, and plasma AVP levels. Carbachol 65-74 vasopressin-neurophysin 2-copeptin Ovis aries 153-156 9688612-8 1998 We suggest that the Ca2+ influx-dependent regulation of the sustained KCa current in CCh-stimulated HSG cells is mediated by the uptake of Ca2+ into the internal Ca2+ store and release via the inositol 1,4,5-trisphosphate-sensitive channel. Carbachol 85-88 casein kappa Homo sapiens 70-73 9720586-7 1998 PTHrP (1-100 nM) relaxed carbachol-contracted bladder body and base by 15% and 45% respectively. Carbachol 25-34 parathyroid hormone like hormone Homo sapiens 0-5 9745899-1 1998 The report describes the results of a study the effect of pH and binding of six physiologically active compounds (isoproterenol, yohimbine, theophylline, propranolol, clonidine and carbachol) on the molecular structure of human serum albumin (HSA) using dynamic light scattering. Carbachol 181-190 albumin Homo sapiens 228-241 9825917-4 1998 However, our results shown that carbachol (a muscarinic acetylcholine receptor agonist), and ligands to other phospholipase C-linked receptors, promoted a rapid increase in the tyrosine phosphorylation of protein kinase Cdelta (PKCdelta), a member of the PKC family of proteins. Carbachol 32-41 protein kinase C, delta Rattus norvegicus 205-226 9825917-4 1998 However, our results shown that carbachol (a muscarinic acetylcholine receptor agonist), and ligands to other phospholipase C-linked receptors, promoted a rapid increase in the tyrosine phosphorylation of protein kinase Cdelta (PKCdelta), a member of the PKC family of proteins. Carbachol 32-41 protein kinase C, delta Rattus norvegicus 228-236 9825917-4 1998 However, our results shown that carbachol (a muscarinic acetylcholine receptor agonist), and ligands to other phospholipase C-linked receptors, promoted a rapid increase in the tyrosine phosphorylation of protein kinase Cdelta (PKCdelta), a member of the PKC family of proteins. Carbachol 32-41 protein kinase C, delta Rattus norvegicus 228-231 9825926-6 1998 VIP thus serves to decrease the EC50 for carbachol nearly three-fold. Carbachol 41-50 vasoactive intestinal peptide Rattus norvegicus 0-3 9825926-7 1998 Results are thus far consistent with the hypothesis that a sustained rise in the intracellular concentration of calcium ions induced by VIP accounts for synergistic secretion and the heightened sensitivity to carbachol. Carbachol 209-218 vasoactive intestinal peptide Rattus norvegicus 136-139 9877233-2 1998 It was observed that the NO synthase (NOS) inhibitor N(G)-nitro-L-arginine methylester (L-NAME) markedly potentiated insulin release and modestly inhibited glucagon release induced by carbachol. Carbachol 184-193 nitric oxide synthase 2 Homo sapiens 25-36 9877233-8 1998 Further, in the presence of diazoxide, a potent K+ ATP-channel opener, plus a depolarizing concentration of K+ the NOS-inhibitor L-NAME still markedly potentiated carbachol-induced insulin release and inhibited glucagon release. Carbachol 163-172 insulin Homo sapiens 181-188 9877233-12 1998 Furthermore, a series of perifusion experiments revealed that hydroxylamine greatly inhibited carbachol-induced insulin release without affecting the 45Ca2+ -efflux pattern. Carbachol 94-103 insulin Homo sapiens 112-119 9877233-13 1998 In summary, our results suggest that the inhibitory effect of NO on carbachol-induced insulin release is not to any significant extent exerted on the IP3-Ca2+ messenger system but rather through S-nitrosylation of critical thiol-residues in protein kinase C and/or other secretion-regulatory thiol groups. Carbachol 68-77 insulin Homo sapiens 86-93 9694941-2 1998 Bethanechol and carbachol produce dose-dependent increases in rat adrenal TH activity. Carbachol 16-25 tyrosine hydroxylase Rattus norvegicus 74-76 9678662-4 1998 Stimulation of the VH (defined as the ventral CA1 and its borders, ventral subiculum, and entorhinal cortex) with the cholinergic agonist carbachol was found to elevate locomotor activity, while pretreatment with the D2 receptor antagonist haloperidol blocked this effect. Carbachol 138-147 carbonic anhydrase 1 Rattus norvegicus 46-49 9681447-2 1998 Serendipitously, however, we observed that pretreatment of Chinese hamster ovary (CHO) cells, which coexpress muscarinic M1 receptors and nNOS, with 3.3 microM or 1 mM carbachol (CCh) for 48 h resulted in marked enhancement of maximal muscarinic receptor-stimulated nNOS activity as determined by L-[3H]citrulline and cyclic [3H]GMP production. Carbachol 168-177 nitric oxide synthase 1, neuronal Mus musculus 138-142 9681447-2 1998 Serendipitously, however, we observed that pretreatment of Chinese hamster ovary (CHO) cells, which coexpress muscarinic M1 receptors and nNOS, with 3.3 microM or 1 mM carbachol (CCh) for 48 h resulted in marked enhancement of maximal muscarinic receptor-stimulated nNOS activity as determined by L-[3H]citrulline and cyclic [3H]GMP production. Carbachol 168-177 nitric oxide synthase 1, neuronal Mus musculus 266-270 9681447-6 1998 It is interesting that ionomycin-stimulated nNOS activity was greater in CCh-pretreated cells. Carbachol 73-76 nitric oxide synthase 1, neuronal Mus musculus 44-48 9655696-3 1998 The NO-donor SIN-1 and the cGMP analogs were able to inhibit contractions induced by activation of L-type Ca2+ channels (BAY-K-8644), by carbachol (CCh), and by cyclopiazonic acid (CPA), a blocker of sarcoplasmic Ca2+-ATPase. Carbachol 137-146 MAPK associated protein 1 Homo sapiens 13-18 9655696-3 1998 The NO-donor SIN-1 and the cGMP analogs were able to inhibit contractions induced by activation of L-type Ca2+ channels (BAY-K-8644), by carbachol (CCh), and by cyclopiazonic acid (CPA), a blocker of sarcoplasmic Ca2+-ATPase. Carbachol 148-151 MAPK associated protein 1 Homo sapiens 13-18 9683560-5 1998 Administration of angiotensin II as well as endothelin into the observation chamber caused a significant decrease of the mean capillary diameter (13 and 16% reduction, respectively) within 90 s. Carbachol, bradykinin, and histamine significantly increased the capillary diameter within 90 s (13, 20, and 18% increase, respectively). Carbachol 195-204 angiotensinogen Rattus norvegicus 18-32 9683731-5 1998 In rectal gland slices sgk-1 transcription was induced by exposure to hypertonic solution, reduction of the extracellular urea concentration, and addition of the secretagogues vasoactive intestinal polypeptide (VIP) and carbachol. Carbachol 220-229 serum/glucocorticoid regulated kinase 1 Homo sapiens 23-28 9636140-4 1998 This report demonstrates that tyrosine phosphorylation of paxillin and FAK elicited by stimulation of muscarinic m3 receptors with the acetylcholine analog carbachol is inhibited by soluble peptides containing the arginine-glycine-aspartate motif (the recognition site for integrins found in adhesion proteins such as fibronectin) but is unaffected by peptides containing the inactive sequence arginine-glycine-glutamate. Carbachol 156-165 protein tyrosine kinase 2 Homo sapiens 71-74 9787266-0 1998 Serotonin and carbachol induced suppression of synaptic responses in rat CA1 hippocampal area: effects of corticosteroid receptor activation in vivo. Carbachol 14-23 carbonic anhydrase 1 Rattus norvegicus 73-76 9787266-1 1998 Previous studies have shown that corticosteroids affect the changes in membrane potential evoked in CA1 hippocampal neurons by serotonin and the metabolically stable cholinergic analogue carbachol: Low corticosteroid levels induced by steroid administration to adrenalectomized rats or obtained in adrenally intact rats were associated with small transmitter responses. Carbachol 187-196 carbonic anhydrase 1 Rattus norvegicus 100-103 9667520-3 1998 Amylase and lipase secretion was measured from isolated pancreatic acini in response to CCK-OP (10(-12)-10(-8) M) and carbachol (10(-8)-10(-3) M). Carbachol 118-127 lipase G, endothelial type Rattus norvegicus 12-18 9667520-8 1998 Lipase secretion increased in response to CCK-OP in AI compared to PFC and FAC rats but was similar in all three groups in response to carbachol. Carbachol 135-144 lipase G, endothelial type Rattus norvegicus 0-6 9636140-4 1998 This report demonstrates that tyrosine phosphorylation of paxillin and FAK elicited by stimulation of muscarinic m3 receptors with the acetylcholine analog carbachol is inhibited by soluble peptides containing the arginine-glycine-aspartate motif (the recognition site for integrins found in adhesion proteins such as fibronectin) but is unaffected by peptides containing the inactive sequence arginine-glycine-glutamate. Carbachol 156-165 fibronectin 1 Homo sapiens 318-329 9690052-12 1998 Parasympathetic cholinergic influence was studied using 500 mumol/l carbamylcholine, which increased insulin secretion. Carbachol 68-83 insulin Homo sapiens 101-108 9636140-6 1998 The response to carbachol is dependent on the presence of fibronectin. Carbachol 16-25 fibronectin 1 Homo sapiens 58-69 9690869-17 1998 The similar beta3-mRNA levels in ileum of obese and lean mice were associated with indistinguishable responses of carbachol-contracted ileum to a beta3-agonist and similar affinity for beta-antagonists. Carbachol 114-123 calcium channel, voltage-dependent, beta 3 subunit Mus musculus 12-17 9690869-17 1998 The similar beta3-mRNA levels in ileum of obese and lean mice were associated with indistinguishable responses of carbachol-contracted ileum to a beta3-agonist and similar affinity for beta-antagonists. Carbachol 114-123 histocompatibility 2, P region beta locus Mus musculus 144-151 9560440-1 1998 The possibility of the protein kinase C (PKC) pathway being a mechanism underlying the desensitization of carbachol- (CCh-)activated nonselective cationic current (ICCh) was investigated in a study of guinea-pig gastric myocytes. Carbachol 106-115 Prkca Cavia porcellus 23-39 9560440-1 1998 The possibility of the protein kinase C (PKC) pathway being a mechanism underlying the desensitization of carbachol- (CCh-)activated nonselective cationic current (ICCh) was investigated in a study of guinea-pig gastric myocytes. Carbachol 106-115 Prkca Cavia porcellus 41-44 9622444-0 1998 Carbachol-stimulated Ca2+ increase in single neuroblastoma SH-SY5Y cells: effects of ethanol. Carbachol 0-9 carbonic anhydrase 2 Homo sapiens 21-24 9622444-1 1998 The effect of ethanol on the characteristics of carbachol-stimulated release of Ca2+ from intracellular Ca2+ stores was studied in single SH-SY5Y cells. Carbachol 48-57 carbonic anhydrase 2 Homo sapiens 80-83 9622444-1 1998 The effect of ethanol on the characteristics of carbachol-stimulated release of Ca2+ from intracellular Ca2+ stores was studied in single SH-SY5Y cells. Carbachol 48-57 carbonic anhydrase 2 Homo sapiens 104-107 9622444-2 1998 Stimulation with carbachol, in the absence of extracellular Ca2+, elicited a rapid Ca2+ increase in SH-SY5Y cells peaking within seconds after addition of maximal agonist concentration. Carbachol 17-26 carbonic anhydrase 2 Homo sapiens 83-86 9622444-5 1998 In a carbachol dose-response analysis, the EC50 for the number of responsive cells and for the peak [Ca2+]i response was lower than that for carbachol-induced inositol 1,4,5-trisphosphate formation by a factor of 5 to 50. Carbachol 5-14 carbonic anhydrase 2 Homo sapiens 101-104 9751139-5 1998 Phosphorylation of FAK+ was markedly increased by carbachol and LPA. Carbachol 50-59 protein tyrosine kinase 2 Rattus norvegicus 19-22 9579481-11 1998 Stimulation of a receptor with PTK activity by EGF induced a relaxation in trabecular meshwork and a contraction in ciliary muscle precontracted by carbachol. Carbachol 148-157 LOC521832 Bos taurus 47-50 9582440-5 1998 Carbachol or nicotine increased expression of transfected FGF-2 gene promoter-luciferase constructs and were more potent than the muscarinic agonist ABMCB. Carbachol 0-9 fibroblast growth factor 2 Bos taurus 58-63 9604867-7 1998 Thus the carbachol activation of PKC appeared also to be dissociated from the stimulation of insulin release. Carbachol 9-18 protein kinase C, alpha Rattus norvegicus 33-36 9604867-8 1998 However, when the activation of several different PKC isozymes was studied, an atypical PKC isozyme, zeta, was found to be translocated by carbachol. Carbachol 139-148 protein kinase C, alpha Rattus norvegicus 50-53 9604867-8 1998 However, when the activation of several different PKC isozymes was studied, an atypical PKC isozyme, zeta, was found to be translocated by carbachol. Carbachol 139-148 protein kinase C, alpha Rattus norvegicus 88-91 9604867-9 1998 By Western blotting analysis, carbachol selectively translocated the conventional PKC isozymes alpha and beta (the activation of which is dependent on Ca2+ and DAG) from the cytosol to the membrane. Carbachol 30-39 protein kinase C, alpha Rattus norvegicus 82-85 9604867-15 1998 The data indicate that carbachol-stimulated insulin release in RINm5F cells is mediated to a large degree by the activation of the atypical PKC isozyme zeta. Carbachol 23-32 protein kinase C, alpha Rattus norvegicus 140-143 9488697-7 1998 Thus, VIP and pituitary adenylyl cyclase activating peptide (acting through Gs-coupled pituitary adenylyl cyclase activating peptide-I receptors) were potent stimuli of type I receptor phosphorylation, and remarkably, even slight increases in cAMP concentration induced by carbachol (acting through Gq-coupled muscarinic receptors) or other Ca2+ mobilizing agents were sufficient to cause phosphorylation. Carbachol 273-282 vasoactive intestinal peptide Homo sapiens 6-9 9681185-4 1998 Activation of DM1 receptor in S2-DM1-TRPL cells by 100 microM carbamylcholine induced Ca2+ release from an intracellular Ca2+ pool followed by a Gd(3+)-insensitive Ca2+ influx. Carbachol 62-77 muscarinic Acetylcholine Receptor, A-type Drosophila melanogaster 14-17 9681185-4 1998 Activation of DM1 receptor in S2-DM1-TRPL cells by 100 microM carbamylcholine induced Ca2+ release from an intracellular Ca2+ pool followed by a Gd(3+)-insensitive Ca2+ influx. Carbachol 62-77 DM1 protein kinase Homo sapiens 33-36 9681185-5 1998 Pretreatment of S2-DM1-TRPL cells with 10 microM atropine abolished Gd(3+)-insensitive Ca2+ influx triggered by carbamylcholine, but the response was not blocked by prior incubation with pertussis toxin. Carbachol 112-127 muscarinic Acetylcholine Receptor, A-type Drosophila melanogaster 19-22 9553056-2 1998 This cytoskeletal response is rapid, peaking 2 h after thrombin stimulation, and reverses by 50% after 24 h. The thrombin receptor peptide SFLLRNP also induces cell rounding, whereas other G protein-linked receptor agonists such as carbachol, lysophosphatidic acid, or bradykinin fail to do so. Carbachol 232-241 coagulation factor II, thrombin Homo sapiens 113-121 9553056-7 1998 Thrombin also leads to a rapid, 2.4-fold increase in 32P incorporation into myosin light chain while carbachol does not. Carbachol 101-110 coagulation factor II, thrombin Homo sapiens 0-8 9523591-2 1998 Stimulation with carbachol induced expression of c-fos, fosB, c-jun, junB, and junD. Carbachol 17-26 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 49-54 9523591-2 1998 Stimulation with carbachol induced expression of c-fos, fosB, c-jun, junB, and junD. Carbachol 17-26 FosB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 56-60 9523591-2 1998 Stimulation with carbachol induced expression of c-fos, fosB, c-jun, junB, and junD. Carbachol 17-26 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 62-67 9523591-2 1998 Stimulation with carbachol induced expression of c-fos, fosB, c-jun, junB, and junD. Carbachol 17-26 JunB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 69-73 9523591-2 1998 Stimulation with carbachol induced expression of c-fos, fosB, c-jun, junB, and junD. Carbachol 17-26 JunD proto-oncogene, AP-1 transcription factor subunit Homo sapiens 79-83 9523591-6 1998 Inhibition of protein kinase C with GF109203X suppressed the carbachol-stimulated increase in mRNA levels of c-fos, fosB, and junB by approximately 70% but had only minor effects on the expression of c-jun and junD. Carbachol 61-70 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 109-114 9523591-6 1998 Inhibition of protein kinase C with GF109203X suppressed the carbachol-stimulated increase in mRNA levels of c-fos, fosB, and junB by approximately 70% but had only minor effects on the expression of c-jun and junD. Carbachol 61-70 FosB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 116-120 9523591-6 1998 Inhibition of protein kinase C with GF109203X suppressed the carbachol-stimulated increase in mRNA levels of c-fos, fosB, and junB by approximately 70% but had only minor effects on the expression of c-jun and junD. Carbachol 61-70 JunB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 126-130 9523591-6 1998 Inhibition of protein kinase C with GF109203X suppressed the carbachol-stimulated increase in mRNA levels of c-fos, fosB, and junB by approximately 70% but had only minor effects on the expression of c-jun and junD. Carbachol 61-70 JunD proto-oncogene, AP-1 transcription factor subunit Homo sapiens 210-214 9523591-7 1998 On the other hand, preincubation with KN-62 attenuated the carbachol-induced increase in c-jun and junD expression by 70% but had no effect on c-fos, fosB, and junB mRNA levels. Carbachol 59-68 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 89-94 9523591-7 1998 On the other hand, preincubation with KN-62 attenuated the carbachol-induced increase in c-jun and junD expression by 70% but had no effect on c-fos, fosB, and junB mRNA levels. Carbachol 59-68 JunD proto-oncogene, AP-1 transcription factor subunit Homo sapiens 99-103 9523591-8 1998 Simultaneous inhibition of both protein kinase C and Ca2+/calmodulin-dependent kinase II completely abolished the carbachol-stimulated expression of c-jun and junD, but c-fos, fosB, and junB were still expressed to a certain extent under this condition. Carbachol 114-123 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 149-154 9523591-8 1998 Simultaneous inhibition of both protein kinase C and Ca2+/calmodulin-dependent kinase II completely abolished the carbachol-stimulated expression of c-jun and junD, but c-fos, fosB, and junB were still expressed to a certain extent under this condition. Carbachol 114-123 JunD proto-oncogene, AP-1 transcription factor subunit Homo sapiens 159-163 9523591-8 1998 Simultaneous inhibition of both protein kinase C and Ca2+/calmodulin-dependent kinase II completely abolished the carbachol-stimulated expression of c-jun and junD, but c-fos, fosB, and junB were still expressed to a certain extent under this condition. Carbachol 114-123 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 169-174 9523591-8 1998 Simultaneous inhibition of both protein kinase C and Ca2+/calmodulin-dependent kinase II completely abolished the carbachol-stimulated expression of c-jun and junD, but c-fos, fosB, and junB were still expressed to a certain extent under this condition. Carbachol 114-123 FosB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 176-180 9523591-8 1998 Simultaneous inhibition of both protein kinase C and Ca2+/calmodulin-dependent kinase II completely abolished the carbachol-stimulated expression of c-jun and junD, but c-fos, fosB, and junB were still expressed to a certain extent under this condition. Carbachol 114-123 JunB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 186-190 9514902-5 1998 Concentrations of carbachol and xanomeline producing maximal effects on APPs release reduced the secretion of A beta by 28 and 46%, respectively. Carbachol 18-27 cathepsin B Homo sapiens 72-76 9514902-5 1998 Concentrations of carbachol and xanomeline producing maximal effects on APPs release reduced the secretion of A beta by 28 and 46%, respectively. Carbachol 18-27 amyloid beta precursor protein Homo sapiens 110-116 9478991-7 1998 The rates of both internalization and down-regulation of hm2 receptors in the presence of 10(-6) M or lower concentrations of carbamylcholine were markedly increased by coexpression of GRK2. Carbachol 126-141 cholinergic receptor muscarinic 2 Homo sapiens 57-60 9507142-3 1998 Reversible acetylcholinesterase (AChE) inhibitors typically caused large transient increases in firing that decayed more slowly than responses to carbachol. Carbachol 146-155 acetylcholinesterase Rattus norvegicus 33-37 9498810-2 1998 When stably expressed in HEK293 cells, hTrp3 formed ion channels that were active under resting conditions but could be further stimulated by carbachol or ATP via endogenous muscarinic or purinergic receptors, respectively. Carbachol 142-151 transient receptor potential cation channel subfamily C member 3 Homo sapiens 39-44 9514311-3 1998 Carbachol-stimulated fosB and junB expression was elevated in ethanol-exposed cells compared with control cells. Carbachol 0-9 FosB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 21-25 9514311-3 1998 Carbachol-stimulated fosB and junB expression was elevated in ethanol-exposed cells compared with control cells. Carbachol 0-9 JunB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 30-34 9514311-5 1998 Preincubation with muscarinic antagonists or protein kinase C inhibitor demonstrated that the carbachol-stimulated increase in fosB and junB mRNA levels was primarily mediated via M1 receptors and dependent on the activity of protein kinase C in both control and ethanol-exposed cells. Carbachol 94-103 FosB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 127-131 9514311-5 1998 Preincubation with muscarinic antagonists or protein kinase C inhibitor demonstrated that the carbachol-stimulated increase in fosB and junB mRNA levels was primarily mediated via M1 receptors and dependent on the activity of protein kinase C in both control and ethanol-exposed cells. Carbachol 94-103 JunB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 136-140 9635156-1 1998 We examined the effects of the muscarinic agonist carbachol on ion secretion induced by substance P (SP) in piglet jejunal tissues mounted in Ussing chambers. Carbachol 50-59 tachykinin precursor 1 Homo sapiens 88-99 9466430-1 1998 We investigated the effects of the cholinergic agonist carbachol (25 microM) on the synaptic potentials recorded extracellularly and intracellularly from the CA3 area of immature hippocampal slices of the rat (postnatal days 10-20). Carbachol 55-64 carbonic anhydrase 3 Rattus norvegicus 158-161 9490877-12 1998 Tissue incubation with carbachol or VIP significantly potentiated delta Isc induced by VIP and carbachol, respectively, indicating cross-potentiation. Carbachol 23-32 VIP peptides Cavia porcellus 87-90 9490877-12 1998 Tissue incubation with carbachol or VIP significantly potentiated delta Isc induced by VIP and carbachol, respectively, indicating cross-potentiation. Carbachol 95-104 VIP peptides Cavia porcellus 36-39 9592059-6 1998 Carbachol stimulated 2DG uptake in both G(q alpha) and GLUT1-transfected cells. Carbachol 0-9 solute carrier family 2 member 1 Homo sapiens 55-60 9530149-2 1998 Stimulation of motilin receptors by exogenously applied motilin (1 nM) resulted in a large increase in carbachol (CCh)-induced atropine-sensitive cation current (ICCh) at threshold concentrations of CCh (0.3-1 microM) at 30 degrees C. This potentiation was abolished in the presence of a specific blocker of motilin receptor (GM109) and was attenuated with increased concentrations of either motilin or CCh, being virtually absent with maximally effective concentrations of these agonists. Carbachol 103-112 promotilin Oryctolagus cuniculus 15-22 9530149-2 1998 Stimulation of motilin receptors by exogenously applied motilin (1 nM) resulted in a large increase in carbachol (CCh)-induced atropine-sensitive cation current (ICCh) at threshold concentrations of CCh (0.3-1 microM) at 30 degrees C. This potentiation was abolished in the presence of a specific blocker of motilin receptor (GM109) and was attenuated with increased concentrations of either motilin or CCh, being virtually absent with maximally effective concentrations of these agonists. Carbachol 103-112 promotilin Oryctolagus cuniculus 56-63 9530149-2 1998 Stimulation of motilin receptors by exogenously applied motilin (1 nM) resulted in a large increase in carbachol (CCh)-induced atropine-sensitive cation current (ICCh) at threshold concentrations of CCh (0.3-1 microM) at 30 degrees C. This potentiation was abolished in the presence of a specific blocker of motilin receptor (GM109) and was attenuated with increased concentrations of either motilin or CCh, being virtually absent with maximally effective concentrations of these agonists. Carbachol 103-112 promotilin Oryctolagus cuniculus 56-63 9530149-2 1998 Stimulation of motilin receptors by exogenously applied motilin (1 nM) resulted in a large increase in carbachol (CCh)-induced atropine-sensitive cation current (ICCh) at threshold concentrations of CCh (0.3-1 microM) at 30 degrees C. This potentiation was abolished in the presence of a specific blocker of motilin receptor (GM109) and was attenuated with increased concentrations of either motilin or CCh, being virtually absent with maximally effective concentrations of these agonists. Carbachol 103-112 promotilin Oryctolagus cuniculus 56-63 9530149-2 1998 Stimulation of motilin receptors by exogenously applied motilin (1 nM) resulted in a large increase in carbachol (CCh)-induced atropine-sensitive cation current (ICCh) at threshold concentrations of CCh (0.3-1 microM) at 30 degrees C. This potentiation was abolished in the presence of a specific blocker of motilin receptor (GM109) and was attenuated with increased concentrations of either motilin or CCh, being virtually absent with maximally effective concentrations of these agonists. Carbachol 114-117 promotilin Oryctolagus cuniculus 15-22 9530149-2 1998 Stimulation of motilin receptors by exogenously applied motilin (1 nM) resulted in a large increase in carbachol (CCh)-induced atropine-sensitive cation current (ICCh) at threshold concentrations of CCh (0.3-1 microM) at 30 degrees C. This potentiation was abolished in the presence of a specific blocker of motilin receptor (GM109) and was attenuated with increased concentrations of either motilin or CCh, being virtually absent with maximally effective concentrations of these agonists. Carbachol 114-117 promotilin Oryctolagus cuniculus 56-63 9530149-2 1998 Stimulation of motilin receptors by exogenously applied motilin (1 nM) resulted in a large increase in carbachol (CCh)-induced atropine-sensitive cation current (ICCh) at threshold concentrations of CCh (0.3-1 microM) at 30 degrees C. This potentiation was abolished in the presence of a specific blocker of motilin receptor (GM109) and was attenuated with increased concentrations of either motilin or CCh, being virtually absent with maximally effective concentrations of these agonists. Carbachol 114-117 promotilin Oryctolagus cuniculus 56-63 9530149-2 1998 Stimulation of motilin receptors by exogenously applied motilin (1 nM) resulted in a large increase in carbachol (CCh)-induced atropine-sensitive cation current (ICCh) at threshold concentrations of CCh (0.3-1 microM) at 30 degrees C. This potentiation was abolished in the presence of a specific blocker of motilin receptor (GM109) and was attenuated with increased concentrations of either motilin or CCh, being virtually absent with maximally effective concentrations of these agonists. Carbachol 114-117 promotilin Oryctolagus cuniculus 56-63 9530149-2 1998 Stimulation of motilin receptors by exogenously applied motilin (1 nM) resulted in a large increase in carbachol (CCh)-induced atropine-sensitive cation current (ICCh) at threshold concentrations of CCh (0.3-1 microM) at 30 degrees C. This potentiation was abolished in the presence of a specific blocker of motilin receptor (GM109) and was attenuated with increased concentrations of either motilin or CCh, being virtually absent with maximally effective concentrations of these agonists. Carbachol 163-166 promotilin Oryctolagus cuniculus 15-22 9530149-2 1998 Stimulation of motilin receptors by exogenously applied motilin (1 nM) resulted in a large increase in carbachol (CCh)-induced atropine-sensitive cation current (ICCh) at threshold concentrations of CCh (0.3-1 microM) at 30 degrees C. This potentiation was abolished in the presence of a specific blocker of motilin receptor (GM109) and was attenuated with increased concentrations of either motilin or CCh, being virtually absent with maximally effective concentrations of these agonists. Carbachol 163-166 promotilin Oryctolagus cuniculus 56-63 9530149-2 1998 Stimulation of motilin receptors by exogenously applied motilin (1 nM) resulted in a large increase in carbachol (CCh)-induced atropine-sensitive cation current (ICCh) at threshold concentrations of CCh (0.3-1 microM) at 30 degrees C. This potentiation was abolished in the presence of a specific blocker of motilin receptor (GM109) and was attenuated with increased concentrations of either motilin or CCh, being virtually absent with maximally effective concentrations of these agonists. Carbachol 163-166 promotilin Oryctolagus cuniculus 56-63 9530149-2 1998 Stimulation of motilin receptors by exogenously applied motilin (1 nM) resulted in a large increase in carbachol (CCh)-induced atropine-sensitive cation current (ICCh) at threshold concentrations of CCh (0.3-1 microM) at 30 degrees C. This potentiation was abolished in the presence of a specific blocker of motilin receptor (GM109) and was attenuated with increased concentrations of either motilin or CCh, being virtually absent with maximally effective concentrations of these agonists. Carbachol 163-166 promotilin Oryctolagus cuniculus 56-63 9530149-2 1998 Stimulation of motilin receptors by exogenously applied motilin (1 nM) resulted in a large increase in carbachol (CCh)-induced atropine-sensitive cation current (ICCh) at threshold concentrations of CCh (0.3-1 microM) at 30 degrees C. This potentiation was abolished in the presence of a specific blocker of motilin receptor (GM109) and was attenuated with increased concentrations of either motilin or CCh, being virtually absent with maximally effective concentrations of these agonists. Carbachol 163-166 promotilin Oryctolagus cuniculus 15-22 9530149-2 1998 Stimulation of motilin receptors by exogenously applied motilin (1 nM) resulted in a large increase in carbachol (CCh)-induced atropine-sensitive cation current (ICCh) at threshold concentrations of CCh (0.3-1 microM) at 30 degrees C. This potentiation was abolished in the presence of a specific blocker of motilin receptor (GM109) and was attenuated with increased concentrations of either motilin or CCh, being virtually absent with maximally effective concentrations of these agonists. Carbachol 163-166 promotilin Oryctolagus cuniculus 56-63 9530149-2 1998 Stimulation of motilin receptors by exogenously applied motilin (1 nM) resulted in a large increase in carbachol (CCh)-induced atropine-sensitive cation current (ICCh) at threshold concentrations of CCh (0.3-1 microM) at 30 degrees C. This potentiation was abolished in the presence of a specific blocker of motilin receptor (GM109) and was attenuated with increased concentrations of either motilin or CCh, being virtually absent with maximally effective concentrations of these agonists. Carbachol 163-166 promotilin Oryctolagus cuniculus 56-63 9530149-2 1998 Stimulation of motilin receptors by exogenously applied motilin (1 nM) resulted in a large increase in carbachol (CCh)-induced atropine-sensitive cation current (ICCh) at threshold concentrations of CCh (0.3-1 microM) at 30 degrees C. This potentiation was abolished in the presence of a specific blocker of motilin receptor (GM109) and was attenuated with increased concentrations of either motilin or CCh, being virtually absent with maximally effective concentrations of these agonists. Carbachol 163-166 promotilin Oryctolagus cuniculus 56-63 9484239-6 1998 In the SH-SY5Y cells, both the basal and the carbachol-stimulated release of the amyloid precursor protein were blocked by batimastat. Carbachol 45-54 amyloid beta precursor protein Homo sapiens 81-106 9478991-7 1998 The rates of both internalization and down-regulation of hm2 receptors in the presence of 10(-6) M or lower concentrations of carbamylcholine were markedly increased by coexpression of GRK2. Carbachol 126-141 G protein-coupled receptor kinase 2 Homo sapiens 185-189 9634927-5 1998 For example, 24 h treatment of acinar cells with IL-6 or IFN-gamma did not alter basal or carbachol-stimulated protein (beta-hexosaminidase) secretion, whereas a combination of IL-1 alpha and IL-1 beta decreased carbachol-stimulated beta-hexosaminidase secretion by 80%. Carbachol 212-221 interleukin 1 alpha Mus musculus 177-187 9634927-5 1998 For example, 24 h treatment of acinar cells with IL-6 or IFN-gamma did not alter basal or carbachol-stimulated protein (beta-hexosaminidase) secretion, whereas a combination of IL-1 alpha and IL-1 beta decreased carbachol-stimulated beta-hexosaminidase secretion by 80%. Carbachol 212-221 interleukin 1 beta Mus musculus 192-201 9458722-12 1998 Finally, the effect of the cytosolic phospholipase A2 inhibitor arachidonyltrifluoromethyl ketone (AACOCF3) on the carbachol-induced short-circuit current (Isc) response was determined. Carbachol 115-124 phospholipase A2 group IVA Homo sapiens 27-53 9492905-1 1998 The effects of 3 alpha-hydroxy-5 alpha-pregnan-20-one (allopregnanolone) and carbachol on CA1 and dentate gyrus action potentials were studied in hippocampus slices in premature, follicular and luteal phase rats. Carbachol 77-86 carbonic anhydrase 1 Rattus norvegicus 90-93 9458783-0 1998 Distinct effects of tetragastrin, histamine, and CCh on rat gastric mucin synthesis and contribution of NO. Carbachol 49-52 solute carrier family 13 member 2 Rattus norvegicus 68-73 9458783-1 1998 Although gastrin, histamine, and carbachol (CCh) accelerate gastric mucin metabolism, information about their target cells of mucin production is lacking. Carbachol 33-42 solute carrier family 13 member 2 Rattus norvegicus 68-73 9458783-1 1998 Although gastrin, histamine, and carbachol (CCh) accelerate gastric mucin metabolism, information about their target cells of mucin production is lacking. Carbachol 44-47 solute carrier family 13 member 2 Rattus norvegicus 68-73 9871443-6 1998 In the present study, it will be shown that the co-application of NMDA and carbachol synergistically increases the release of [3H]-DA and that this effect is reduced by mepacrine or 4-bromophenacylbromide (10(-7) M), two inhibitors of PLA2. Carbachol 75-84 phospholipase A2 group IB Homo sapiens 235-239 9458783-6 1998 Both histamine and CCh dose dependently increased 3H- and 14C-labeled corpus mucin. Carbachol 19-22 solute carrier family 13 member 2 Rattus norvegicus 77-82 9458783-7 1998 Only CCh stimulated antral mucin biosynthesis. Carbachol 5-8 solute carrier family 13 member 2 Rattus norvegicus 27-32 9468094-12 1998 In this preparation carbachol (10(-4) M) and neuromedin C (10(-9) M) significantly stimulated gastrin release from 2.6 +/- 0.4 to 4.9 +/- 0.3 and 8.5 +/- 0.9% of the total cellular content, respectively, while GABA (10(-10)-10(-3) M) changed neither basal nor carbachol- and neuromedin C-stimulated gastrin release. Carbachol 20-29 gastrin Rattus norvegicus 94-101 9492905-3 1998 The amplitude of CA1 population spike (POPSP) was reduced by allopregnanolone (-38 +/- 3%) and carbachol (-21 +/- 4%) in the luteal phase slices. Carbachol 95-104 carbonic anhydrase 1 Rattus norvegicus 17-20 9492905-5 1998 The inhibition caused by allopregnanolone and the mixture of allopregnanolone and carbachol in CA1 was significantly larger in the luteal phase than in the follicular phase (P = 0.02 and 0.0002). Carbachol 82-91 carbonic anhydrase 1 Rattus norvegicus 95-98 9852206-5 1998 Stimulation of SH-SY5Y cells with carbachol and KCl shows that noradrenaline and neuropeptide Y are released in the same proportion as stored in the large dense cored vesicles. Carbachol 34-43 neuropeptide Y Homo sapiens 81-95 9468094-12 1998 In this preparation carbachol (10(-4) M) and neuromedin C (10(-9) M) significantly stimulated gastrin release from 2.6 +/- 0.4 to 4.9 +/- 0.3 and 8.5 +/- 0.9% of the total cellular content, respectively, while GABA (10(-10)-10(-3) M) changed neither basal nor carbachol- and neuromedin C-stimulated gastrin release. Carbachol 20-29 gastrin Rattus norvegicus 299-306 9422356-8 1998 On the other hand, prevention of the carbachol-mediated increase of intracellular free Ca2+ by pretreatment with the cell-permeant Ca2+ chelator BAPTA/AM did attenuate the carbachol-mediated increase in cytosolic CaM (221 +/- 37% of control without BAPTA/AM vs. 136 +/- 13% with BAPTA/AM). Carbachol 37-46 calmodulin 1 Homo sapiens 213-216 9422356-8 1998 On the other hand, prevention of the carbachol-mediated increase of intracellular free Ca2+ by pretreatment with the cell-permeant Ca2+ chelator BAPTA/AM did attenuate the carbachol-mediated increase in cytosolic CaM (221 +/- 37% of control without BAPTA/AM vs. 136 +/- 13% with BAPTA/AM). Carbachol 172-181 calmodulin 1 Homo sapiens 213-216 9468094-12 1998 In this preparation carbachol (10(-4) M) and neuromedin C (10(-9) M) significantly stimulated gastrin release from 2.6 +/- 0.4 to 4.9 +/- 0.3 and 8.5 +/- 0.9% of the total cellular content, respectively, while GABA (10(-10)-10(-3) M) changed neither basal nor carbachol- and neuromedin C-stimulated gastrin release. Carbachol 260-269 gastrin Rattus norvegicus 94-101 9422356-14 1998 These data demonstrate that release of Ca2+ from the intracellular store is important for the carbachol-mediated redistribution of CaM in human neuroblastoma SK-N-SH cells. Carbachol 94-103 calmodulin 1 Homo sapiens 131-134 9510054-3 1998 In the present study, we examined the consequences of steroid modulation of these post-synaptic membrane effects and/or possible pre-synaptic effects by 5HT and CCh for the excitability in the CA1 area, using extracellular field potential or intracellular recordings from individual pyramidal neurons. Carbachol 161-164 carbonic anhydrase 1 Rattus norvegicus 193-196 9481674-8 1998 Carbachol induced a biphasic contraction: an initial rapid increase in force (peak 1) followed by a partial relaxation and a second delayed increase in force (peak 2). Carbachol 0-9 pseudopodium-enriched atypical kinase 1 Cavia porcellus 78-84 9459569-4 1998 Furthermore, 5-HT-induced contraction of the rat fundus (5-HT2B) and 5-HT-induced relaxation of the rabbit aorta in the presence of ketanserin (5-HT1) and carbachol-induced contraction of the guinea-pig ileum (muscarinic M3) were not affected by gamma-mangostin (5 microM). Carbachol 155-164 5-hydroxytryptamine receptor 2B Rattus norvegicus 57-63 9382934-7 1998 NPY caused 50% inhibition of the effect of carbachol. Carbachol 43-52 neuropeptide Y Homo sapiens 0-3 9382934-8 1998 Measurements of [Ca2+]i showed that NPY inhibited the carbachol-induced rise in [Ca2+]i, which correlates with the reduced activation of basolateral K+ channels. Carbachol 54-63 neuropeptide Y Homo sapiens 36-39 9700763-3 1998 PACAP-27 had no effect on basal cells but significantly increased the response to histamine (10(-4) M) at 10(-9) M and to carbachol (10(-4) M) in the presence of ranitidine (10(-4) M) at 10(-7) M and 10(-8) M. PACAP (6-38), an antagonist of PACAP, inhibited the effect of PACAP-27 on carbachol-stimulated cells. Carbachol 122-131 LOW QUALITY PROTEIN: pituitary adenylate cyclase-activating polypeptide Oryctolagus cuniculus 0-5 9700763-3 1998 PACAP-27 had no effect on basal cells but significantly increased the response to histamine (10(-4) M) at 10(-9) M and to carbachol (10(-4) M) in the presence of ranitidine (10(-4) M) at 10(-7) M and 10(-8) M. PACAP (6-38), an antagonist of PACAP, inhibited the effect of PACAP-27 on carbachol-stimulated cells. Carbachol 284-293 LOW QUALITY PROTEIN: pituitary adenylate cyclase-activating polypeptide Oryctolagus cuniculus 0-5 9826910-5 1998 Molecular dynamic simulations of the Drosophila muscarinic receptor are presented in the free, carbamylcholine-bound and NMS-bound forms. Carbachol 95-110 muscarinic Acetylcholine Receptor, A-type Drosophila melanogaster 48-67 9435551-4 1997 Of the primary gastric secretagogues, carbachol was the most potent inducer of ERK2 activity. Carbachol 38-47 mitogen-activated protein kinase 1 Canis lupus familiaris 79-83 11382855-0 1998 c-fos Expression in mesopontine noradrenergic and cholinergic neurons of the cat during carbachol-induced active sleep: a double-labeling study. Carbachol 88-97 Fos proto-oncogene, AP-1 transcription factor subunit Felis catus 0-5 11382855-2 1998 To further examine this hypothesis, we sought to determine the pattern of neuronal activation (via c-fos expression) of catecholaminergic and cholinergic neurons in this region during active sleep induced by the pontine microapplication of carbachol (designated as active sleep-carbachol). Carbachol 240-249 Fos proto-oncogene, AP-1 transcription factor subunit Felis catus 99-104 11382855-4 1998 Compared to control cats, active sleep-carbachol cats exhibited a significantly greater number of Fos-expressing neurons in the dorsolateral region of the pons, which encompasses the locus coeruleus, the lateral pontine reticular formation, the peribrachial nuclei and the latero-dorsal and pedunculo-pontine tegmental nuclei. Carbachol 39-48 Fos proto-oncogene, AP-1 transcription factor subunit Felis catus 98-101 11382855-6 1998 A large number of c-fos-expressing neurons in the active sleep-carbachol cats whose neurotransmitter phenotype was not identified suggests that non-catecholaminergic, non-cholinergic neuronal populations in mesopontine regions are involved in the generation and maintenance of active sleep. Carbachol 63-72 Fos proto-oncogene, AP-1 transcription factor subunit Felis catus 18-23 9510054-0 1998 Serotonin and carbachol induced suppression of synaptic excitability in rat CA1 hippocampal area: effects of corticosteroid receptor activation. Carbachol 14-23 carbonic anhydrase 1 Rattus norvegicus 76-79 9510054-1 1998 Serotonin (5HT) and the cholinergic analogue carbachol (CCh) act on neurons in the hippocampal CA1 area through pre- and post-synaptic receptors. Carbachol 45-54 carbonic anhydrase 1 Rattus norvegicus 95-98 9510054-1 1998 Serotonin (5HT) and the cholinergic analogue carbachol (CCh) act on neurons in the hippocampal CA1 area through pre- and post-synaptic receptors. Carbachol 56-59 carbonic anhydrase 1 Rattus norvegicus 95-98 9510054-5 1998 In slices from adrenally intact rats, both 5HT (3-30 microM) and CCh (1-10 microM) induced a dose-dependent suppression of the synaptic field responses evoked in the CA1 area by stimulation of the Schaffer collaterals. Carbachol 65-68 carbonic anhydrase 1 Rattus norvegicus 166-169 9459616-3 1997 In fact, on SK-N-SH neuroblastoma cells, IL-1beta can inhibit the Ca++ increase induced by stimulation of acetylcholine receptors with carbachol. Carbachol 135-144 interleukin 1 beta Homo sapiens 41-49 9459616-4 1997 In parallel to IL-1beta, the neurotrophic factor CNTF also shows an inhibitory effect on carbachol-stimulated Ca++ increase in CNTFRalpha-expressing SK-N-SH cells. Carbachol 89-98 ciliary neurotrophic factor Homo sapiens 49-53 9459616-4 1997 In parallel to IL-1beta, the neurotrophic factor CNTF also shows an inhibitory effect on carbachol-stimulated Ca++ increase in CNTFRalpha-expressing SK-N-SH cells. Carbachol 89-98 ciliary neurotrophic factor receptor Homo sapiens 127-137 9418962-2 1997 Activation of this neurotransmitter receptor by the stable acetylcholine analog carbachol (CCh) triggers transducing events, modulating c-fos expression and cellular proliferation. Carbachol 80-89 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 136-141 9418962-2 1997 Activation of this neurotransmitter receptor by the stable acetylcholine analog carbachol (CCh) triggers transducing events, modulating c-fos expression and cellular proliferation. Carbachol 91-94 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 136-141 9418962-4 1997 CCh produced a concentration- and time-dependent increase in MAPK activity (predominantly the p42mapk or ERK2) as determined by in-gel MBP kinase assays. Carbachol 0-3 mitogen activated protein kinase 1 Rattus norvegicus 94-101 9418962-4 1997 CCh produced a concentration- and time-dependent increase in MAPK activity (predominantly the p42mapk or ERK2) as determined by in-gel MBP kinase assays. Carbachol 0-3 mitogen activated protein kinase 1 Rattus norvegicus 105-109 9418962-4 1997 CCh produced a concentration- and time-dependent increase in MAPK activity (predominantly the p42mapk or ERK2) as determined by in-gel MBP kinase assays. Carbachol 0-3 myelin basic protein Rattus norvegicus 135-138 9374490-3 1997 This carbachol effect involves (i) activation of brush border phosphatidylinositol 4,5-bisphosphate-specific phospholipase C (PLC) activity and brush border but not basolateral membrane translocation of PLC-gamma1 (Khurana, S., Kreydiyyeh, S., Aronzon, A., Hoogerwerf, W. A., Rhee, S. G., Donowitz, M., and Cohen, M. E. (1996) Biochem. Carbachol 5-14 LOC100009319 Oryctolagus cuniculus 109-124 9374490-3 1997 This carbachol effect involves (i) activation of brush border phosphatidylinositol 4,5-bisphosphate-specific phospholipase C (PLC) activity and brush border but not basolateral membrane translocation of PLC-gamma1 (Khurana, S., Kreydiyyeh, S., Aronzon, A., Hoogerwerf, W. A., Rhee, S. G., Donowitz, M., and Cohen, M. E. (1996) Biochem. Carbachol 5-14 LOC100009319 Oryctolagus cuniculus 126-129 9374490-3 1997 This carbachol effect involves (i) activation of brush border phosphatidylinositol 4,5-bisphosphate-specific phospholipase C (PLC) activity and brush border but not basolateral membrane translocation of PLC-gamma1 (Khurana, S., Kreydiyyeh, S., Aronzon, A., Hoogerwerf, W. A., Rhee, S. G., Donowitz, M., and Cohen, M. E. (1996) Biochem. Carbachol 5-14 LOC100009319 Oryctolagus cuniculus 203-206 9435551-7 1997 PD-98059, a selective inhibitor of the upstream ERK activator mitogen-activated protein kinase/ERK kinase, dose-dependently inhibited both carbachol- and EGF-stimulated ERK2 activity, with a maximal effect observed between 50 and 100 microM. Carbachol 139-148 mitogen-activated protein kinase 1 Canis lupus familiaris 169-173 9435551-11 1997 Acute inhibition of the ERKs by PD-98059 led to a small increase in AP uptake and a complete reversal of the acute inhibitory effect of EGF on AP uptake induced by either carbachol or histamine. Carbachol 171-180 epidermal growth factor Canis lupus familiaris 136-139 9435551-12 1997 In contrast, exposure of the cells to PD-98059 for 16 h led to a reversal of the chronic stimulatory effect of EGF on AP uptake induced by carbachol. Carbachol 139-148 epidermal growth factor Canis lupus familiaris 111-114 9368034-7 1997 Transient expression of mTrp6 in COS.M6 cells by transfection of the full-length mTrp6 cDNA increases Ca2+ entry induced by stimulation of co-transfected M5 muscarinic acetylcholine receptor with carbachol (CCh), as seen by dual wavelength fura 2 fluorescence ratio measurements. Carbachol 196-205 transient receptor potential cation channel, subfamily C, member 6 Mus musculus 24-29 9368034-7 1997 Transient expression of mTrp6 in COS.M6 cells by transfection of the full-length mTrp6 cDNA increases Ca2+ entry induced by stimulation of co-transfected M5 muscarinic acetylcholine receptor with carbachol (CCh), as seen by dual wavelength fura 2 fluorescence ratio measurements. Carbachol 196-205 transient receptor potential cation channel, subfamily C, member 6 Mus musculus 81-86 9368034-7 1997 Transient expression of mTrp6 in COS.M6 cells by transfection of the full-length mTrp6 cDNA increases Ca2+ entry induced by stimulation of co-transfected M5 muscarinic acetylcholine receptor with carbachol (CCh), as seen by dual wavelength fura 2 fluorescence ratio measurements. Carbachol 207-210 transient receptor potential cation channel, subfamily C, member 6 Mus musculus 24-29 9368034-7 1997 Transient expression of mTrp6 in COS.M6 cells by transfection of the full-length mTrp6 cDNA increases Ca2+ entry induced by stimulation of co-transfected M5 muscarinic acetylcholine receptor with carbachol (CCh), as seen by dual wavelength fura 2 fluorescence ratio measurements. Carbachol 207-210 transient receptor potential cation channel, subfamily C, member 6 Mus musculus 81-86 9348338-1 1997 Exposure of rat hippocampal slices to low concentrations of the muscarinic agonist carbachol (CCh) has been shown to produce a slow onset long-term potentiation (LTP) of reactivity to afferent stimulation in CA1 neurons. Carbachol 83-92 carbonic anhydrase 1 Rattus norvegicus 208-211 9348338-1 1997 Exposure of rat hippocampal slices to low concentrations of the muscarinic agonist carbachol (CCh) has been shown to produce a slow onset long-term potentiation (LTP) of reactivity to afferent stimulation in CA1 neurons. Carbachol 94-97 carbonic anhydrase 1 Rattus norvegicus 208-211 9439833-1 1997 The effects of carbamylcholine (CCh) on the gene expression of the neuropeptide vasoactive intestinal polypeptide (VIP) were studied using two human neuroblastoma cell lines. Carbachol 15-30 vasoactive intestinal peptide Homo sapiens 115-118 9439833-1 1997 The effects of carbamylcholine (CCh) on the gene expression of the neuropeptide vasoactive intestinal polypeptide (VIP) were studied using two human neuroblastoma cell lines. Carbachol 32-35 vasoactive intestinal peptide Homo sapiens 115-118 9439833-3 1997 CCh caused a fast increase in VIP mRNA level in both cell lines which was followed by an increase in VIP immunoreactivity. Carbachol 0-3 vasoactive intestinal peptide Homo sapiens 30-33 9439833-3 1997 CCh caused a fast increase in VIP mRNA level in both cell lines which was followed by an increase in VIP immunoreactivity. Carbachol 0-3 vasoactive intestinal peptide Homo sapiens 101-104 9439833-8 1997 Experiments with the translational inhibitor, cycloheximide, showed that CCh mediated a direct effect on the VIP gene expression. Carbachol 73-76 vasoactive intestinal peptide Homo sapiens 109-112 9439833-11 1997 After CCh induction the concentration of all prepro VIP-derived products increased, and there was a tendency towards a shift to more fully processed VIP. Carbachol 6-9 vasoactive intestinal peptide Homo sapiens 52-55 9374680-6 1997 Furthermore, when IL-1 beta-treated islets were exposed to 100 microM carbachol (which activates PLC partially independent of extracellular Ca2+), the effects were still obliterated by IL-1 beta. Carbachol 70-79 interleukin 1 beta Homo sapiens 18-27 9374490-11 1997 Carbachol also increases the amount of tyrosine-phosphorylated villin that associates with PLC-gamma1. Carbachol 0-9 LOC100009319 Oryctolagus cuniculus 91-94 9374490-12 1997 These studies demonstrate that carbachol effects on NaCl absorption are accompanied by an increase in brush border PLC-gamma1 association with villin and an increase in tyrosine phosphorylation of villin. Carbachol 31-40 LOC100009319 Oryctolagus cuniculus 115-118 9374680-6 1997 Furthermore, when IL-1 beta-treated islets were exposed to 100 microM carbachol (which activates PLC partially independent of extracellular Ca2+), the effects were still obliterated by IL-1 beta. Carbachol 70-79 interleukin 1 beta Homo sapiens 185-194 9401759-5 1997 The potency order for the affinity of these agents for muscarinic receptors was carbachol > McN-A-343 >> AHR-602. Carbachol 80-89 aryl hydrocarbon receptor Cavia porcellus 114-117 9374719-3 1997 We have investigated phosphorylation of HSP27 in airway smooth muscle in response to the muscarinic agonist carbachol. Carbachol 108-117 heat shock protein family B (small) member 1 Canis lupus familiaris 40-45 9374719-4 1997 Carbachol increased 32P incorporation into canine tracheal HSP27 and induced a shift in the distribution of charge isoforms on two-dimensional gels to more acidic, phosphorylated forms. Carbachol 0-9 heat shock protein family B (small) member 1 Canis lupus familiaris 59-64 9374719-7 1997 Recombinant canine HSP27 expressed in Escherichia coli was a substrate for MAPKAP kinase-2 in vitro as well as a substrate for endogenous smooth muscle HSP27 kinase, which was activated by carbachol. Carbachol 189-198 heat shock protein family B (small) member 1 Canis lupus familiaris 19-24 9374719-7 1997 Recombinant canine HSP27 expressed in Escherichia coli was a substrate for MAPKAP kinase-2 in vitro as well as a substrate for endogenous smooth muscle HSP27 kinase, which was activated by carbachol. Carbachol 189-198 heat shock protein family B (small) member 1 Canis lupus familiaris 152-157 9401759-7 1997 In the presence of 2.2 mM extracellular Ca2+, McN-A-343 and AHR-602 induced contraction corresponding to 79 and 85%, respectively, of the maximal contraction to 0.1 mM carbachol. Carbachol 168-177 aryl hydrocarbon receptor Cavia porcellus 60-63 9401759-12 1997 Application of McN-A-343 or AHR-602 inhibited the carbachol-induced contraction in Ca(2+)-free solution, and this inhibition was surmounted by a higher concentration of carbachol. Carbachol 50-59 aryl hydrocarbon receptor Cavia porcellus 28-31 9401759-12 1997 Application of McN-A-343 or AHR-602 inhibited the carbachol-induced contraction in Ca(2+)-free solution, and this inhibition was surmounted by a higher concentration of carbachol. Carbachol 169-178 aryl hydrocarbon receptor Cavia porcellus 28-31 9375582-0 1997 Actions of C-type natriuretic peptide and sodium nitroprusside on carbachol-stimulated inositol phosphate formation and contraction in ciliary and iris sphincter smooth muscles. Carbachol 66-75 natriuretic peptide C Bos taurus 11-37 9349533-7 1997 This segregation of InsP3R types explains a previous observation, showing that the muscarinic agonist carbachol causes the reduction or "down-regulation" of type I but not type II InsP3Rs. Carbachol 102-111 inositol 1,4,5-trisphosphate receptor, type 1 Rattus norvegicus 20-26 9349535-5 1997 Incubation with carbachol, a cholinergic agonist known to activate PKC and increase intracellular calcium levels via phosphatidylinositol breakdown, also down-regulated CRF-R1 mRNA levels. Carbachol 16-25 corticotropin releasing hormone receptor 1 Mus musculus 169-175 9356412-5 1997 In extracellular recordings, a regularly spaced bursting pattern of field potentials was observed in both CA3 and CA1 subfields in the presence of carbachol. Carbachol 147-156 carbonic anhydrase 3 Rattus norvegicus 106-109 9356412-5 1997 In extracellular recordings, a regularly spaced bursting pattern of field potentials was observed in both CA3 and CA1 subfields in the presence of carbachol. Carbachol 147-156 carbonic anhydrase 1 Rattus norvegicus 114-117 9356412-10 1997 In the presence of carbachol, individual CA3 pyramidal cells exhibited a slow, rhythmic intrinsic oscillation that was not blocked by DNQX and that was enhanced by membrane hyperpolarization. Carbachol 19-28 carbonic anhydrase 3 Rattus norvegicus 41-44 9300429-6 1997 Carbachol activation of parafascicular neurons also induced the synthesis of c-Fos-like immunoreactive proteins in the pallidal neurons. Carbachol 0-9 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 77-82 9362243-6 1997 Phenylephrine or carbachol inhibited Kv4 currents only when coexpressed, respectively, with alpha1C-adrenergic or M1 muscarinic receptors (this inhibition was also prevented by chelerythrine). Carbachol 17-26 potassium voltage-gated channel subfamily A member 4 Rattus norvegicus 37-40 9430424-9 1997 The maximal tension, frequency and dose-dependency of carbachol-induced contractions were not influenced by motilin (pEC50, carbachol: 6.48 +/- 0.06 (control), 6.49 +/- 0.07 (motilin)). Carbachol 54-63 promotilin Oryctolagus cuniculus 175-182 9362309-5 1997 Therefore, the present study tested the hypothesis that cAMP and PKA within the mPRF modulate the carbachol-induced REM sleep-like state. Carbachol 98-107 Spi-C transcription factor (Spi-1/PU.1 related) Mus musculus 80-84 9375582-10 1997 In bovine Sph, CNP increased cGMP accumulation in a time- and dose-dependent manner and dose dependently inhibited CCh-induced IP3 production and contraction. Carbachol 115-118 natriuretic peptide C Bos taurus 15-18 9375582-12 1997 C-type natriuretic peptide attenuated CCh-induced contraction in CM isolated from monkey and human, but it had no influence on this response in CM isolated from cows, cats, and dogs. Carbachol 38-41 natriuretic peptide C Homo sapiens 0-26 9375582-14 1997 Agents that strongly increase intracellular cGMP levels, including SNP and CNP, produce significant inhibition of CCh-induced IP3 production and contraction. Carbachol 114-117 natriuretic peptide C Bos taurus 75-78 9324135-5 1997 Centrally administered neurotensin inhibits basal, pentagastrin-, carbachol-, and 2-deoxy-D-glucose-induced but not histamine-induced gastric acid secretion. Carbachol 66-75 neurotensin Rattus norvegicus 23-34 9406932-0 1997 AP-1 and NF-kappaB stimulated by carbachol in human neuroblastoma SH-SY5Y cells are differentially sensitive to inhibition by lithium. Carbachol 33-42 JunB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 0-4 9406932-0 1997 AP-1 and NF-kappaB stimulated by carbachol in human neuroblastoma SH-SY5Y cells are differentially sensitive to inhibition by lithium. Carbachol 33-42 nuclear factor kappa B subunit 1 Homo sapiens 9-18 9406932-2 1997 The cholinergic agonist carbachol concentration-dependently stimulated AP-1 (EC50 = 2 microM) and NF-kappaB (EC50 = 14 microM). Carbachol 24-33 JunB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 71-75 9406932-2 1997 The cholinergic agonist carbachol concentration-dependently stimulated AP-1 (EC50 = 2 microM) and NF-kappaB (EC50 = 14 microM). Carbachol 24-33 nuclear factor kappa B subunit 1 Homo sapiens 98-107 9406932-3 1997 Pretreatment for 24 h with a therapeutically relevant concentration of lithium (1 mM) substantially inhibited (30-35%) carbachol-stimulation AP-1 but not NF-kappaB. Carbachol 119-128 JunB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 141-145 9406932-4 1997 Inhibition of carbachol-induced AP-1 was directly related to the concentration of lithium (1-20 mM). Carbachol 14-23 JunB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 32-36 9406932-5 1997 Besides being differentially sensitive to inhibition by lithium, activation of AP-1 and NF-kappaB demonstrated different carbachol EC50 concentrations, and carbachol-induced activation of AP-1, but not NF-kappaB, was inhibited by treating cells with Ni2+, which blocks receptor-mediated calcium influx. Carbachol 121-130 JunB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 79-83 9406932-5 1997 Besides being differentially sensitive to inhibition by lithium, activation of AP-1 and NF-kappaB demonstrated different carbachol EC50 concentrations, and carbachol-induced activation of AP-1, but not NF-kappaB, was inhibited by treating cells with Ni2+, which blocks receptor-mediated calcium influx. Carbachol 121-130 nuclear factor kappa B subunit 1 Homo sapiens 88-97 9406932-5 1997 Besides being differentially sensitive to inhibition by lithium, activation of AP-1 and NF-kappaB demonstrated different carbachol EC50 concentrations, and carbachol-induced activation of AP-1, but not NF-kappaB, was inhibited by treating cells with Ni2+, which blocks receptor-mediated calcium influx. Carbachol 156-165 JunB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 188-192 9406932-5 1997 Besides being differentially sensitive to inhibition by lithium, activation of AP-1 and NF-kappaB demonstrated different carbachol EC50 concentrations, and carbachol-induced activation of AP-1, but not NF-kappaB, was inhibited by treating cells with Ni2+, which blocks receptor-mediated calcium influx. Carbachol 156-165 nuclear factor kappa B subunit 1 Homo sapiens 202-211 9352075-3 1997 In contrast, carbachol (CCH) and A23187 decreased CREB phosphorylation, but CCH did not decrease it in the absence of extracellular Ca2+. Carbachol 13-22 cAMP responsive element binding protein 1 Homo sapiens 50-54 9352075-3 1997 In contrast, carbachol (CCH) and A23187 decreased CREB phosphorylation, but CCH did not decrease it in the absence of extracellular Ca2+. Carbachol 24-27 cAMP responsive element binding protein 1 Homo sapiens 50-54 9487089-4 1997 administration of carbachol (10(-9) and 10(-8) mol/kg) produced decrease in arterial plasma ACE activity in dose-dependent manner (from 40 to 20 nmol/ml.min) and diminution of lung ACE activity (on 18%). Carbachol 18-27 angiotensin I converting enzyme Rattus norvegicus 92-95 9487089-4 1997 administration of carbachol (10(-9) and 10(-8) mol/kg) produced decrease in arterial plasma ACE activity in dose-dependent manner (from 40 to 20 nmol/ml.min) and diminution of lung ACE activity (on 18%). Carbachol 18-27 angiotensin I converting enzyme Rattus norvegicus 181-184 9487089-5 1997 It is suggested that carbachol produces an inactivation and inhibition of ACE secretion by lung and lung ACE biosynthesis in sympathetic overactivity rats. Carbachol 21-30 angiotensin I converting enzyme Rattus norvegicus 74-77 9487089-5 1997 It is suggested that carbachol produces an inactivation and inhibition of ACE secretion by lung and lung ACE biosynthesis in sympathetic overactivity rats. Carbachol 21-30 angiotensin I converting enzyme Rattus norvegicus 105-108 9309207-0 1997 A9 fibroblasts transfected with the m3 muscarinic receptor clone express a Ca2+ channel activated by carbachol, GTP and GDP. Carbachol 101-110 cholinergic receptor muscarinic 3 Homo sapiens 36-58 9376222-5 1997 Both CCh-induced IP3 production and CCh-induced [Ca2+]i mobilization were more potently antagonized by 4-DAMP, an M3 muscarinic receptor antagonist, than by pirenzepine, an M1 receptor antagonist, suggesting that both responses are mediated through the M3 receptor subtype. Carbachol 5-8 cholinergic receptor muscarinic 3 Homo sapiens 114-136 9376222-5 1997 Both CCh-induced IP3 production and CCh-induced [Ca2+]i mobilization were more potently antagonized by 4-DAMP, an M3 muscarinic receptor antagonist, than by pirenzepine, an M1 receptor antagonist, suggesting that both responses are mediated through the M3 receptor subtype. Carbachol 36-39 cholinergic receptor muscarinic 3 Homo sapiens 114-136 9322224-3 1997 Stimulation in this region by carbachol produced natriuresis accompanied by a dramatic increase in plasma ANP concentrations and increased content of the peptide in medial basal hypothalamus (MBH), neurohypophysis (NH) and anterior pituitary gland (AP), without alterations in the content of ANP in lungs or atria. Carbachol 30-39 natriuretic peptide A Rattus norvegicus 106-109 9277413-6 1997 Carbachol, an analog of acetylcholine, and the neuroendocrine peptides somatostatin and vasoactive intestinal polypeptide (VIP) stimulated increased expression of hITF mRNA within 5 min. Carbachol 0-9 vasoactive intestinal peptide Homo sapiens 123-126 9322224-3 1997 Stimulation in this region by carbachol produced natriuresis accompanied by a dramatic increase in plasma ANP concentrations and increased content of the peptide in medial basal hypothalamus (MBH), neurohypophysis (NH) and anterior pituitary gland (AP), without alterations in the content of ANP in lungs or atria. Carbachol 30-39 natriuretic peptide A Rattus norvegicus 292-295 9313780-3 1997 Chick heart cells treated with TGF-beta 1 exhibited a decreased sensitivity for carbachol-mediated inhibition of adenylyl cyclase activity compared with control cells. Carbachol 80-89 transforming growth factor beta 1 Gallus gallus 31-41 9252389-4 1997 Using NIH 3T3 cells expressing G protein-coupled m1 acetylcholine receptors as an experimental model, we have recently shown that the cholinergic agonist carbachol, but not platelet-derived growth factor, potently elevates JNK activity. Carbachol 154-163 mitogen-activated protein kinase 8 Mus musculus 223-226 9252389-5 1997 Consistent with these findings, carbachol, but not platelet-derived growth factor, increased the activity of a c-jun promoter-driven reporter gene (for chloramphenicol acetyltransferase). Carbachol 32-41 jun proto-oncogene Mus musculus 111-116 9252389-10 1997 Furthermore, cotransfection with MEF2C and MEF2D cDNAs potently enhanced the activity of the c-jun promoter in response to carbachol, and stimulation of m1 receptors, but not direct JNK activation, induced expression of a MEF2-responsive plasmid. Carbachol 123-132 myocyte enhancer factor 2C Mus musculus 33-38 9252389-10 1997 Furthermore, cotransfection with MEF2C and MEF2D cDNAs potently enhanced the activity of the c-jun promoter in response to carbachol, and stimulation of m1 receptors, but not direct JNK activation, induced expression of a MEF2-responsive plasmid. Carbachol 123-132 myocyte enhancer factor 2D Mus musculus 43-48 9252389-10 1997 Furthermore, cotransfection with MEF2C and MEF2D cDNAs potently enhanced the activity of the c-jun promoter in response to carbachol, and stimulation of m1 receptors, but not direct JNK activation, induced expression of a MEF2-responsive plasmid. Carbachol 123-132 jun proto-oncogene Mus musculus 93-98 9252389-10 1997 Furthermore, cotransfection with MEF2C and MEF2D cDNAs potently enhanced the activity of the c-jun promoter in response to carbachol, and stimulation of m1 receptors, but not direct JNK activation, induced expression of a MEF2-responsive plasmid. Carbachol 123-132 myocyte enhancer factor 2C Mus musculus 33-37 9258410-9 1997 Treatment with SOD and tiron potentiated carbachol stimulated relaxation in ITA and SV. Carbachol 41-50 superoxide dismutase 1 Homo sapiens 15-18 9277413-6 1997 Carbachol, an analog of acetylcholine, and the neuroendocrine peptides somatostatin and vasoactive intestinal polypeptide (VIP) stimulated increased expression of hITF mRNA within 5 min. Carbachol 0-9 trefoil factor 3 Homo sapiens 163-167 9277413-7 1997 These same factors stimulated parallel secretion of the hITF peptide, with maximal stimulation observed at concentrations ranging from 10(-6) M (carbachol and somatostatin) to 10(-7) M (VIP). Carbachol 145-154 trefoil factor 3 Homo sapiens 56-60 9277413-8 1997 Expression and secretion of hITF in response to carbachol, VIP, and somatostatin was independent of production of apomucin. Carbachol 48-57 trefoil factor 3 Homo sapiens 28-32 9211814-4 1997 The magnitudes of the peak and plateau of [Ca2+]i transients induced by carbachol (CCH, 10(-6) mol/l) were greatly enhanced by an acidic pHi and nearly abolished by an alkaline pHi. Carbachol 72-81 glucose-6-phosphate isomerase Homo sapiens 137-140 9221776-11 1997 In association with the enhancement of spike backpropagation, CCh increased the amplitude and duration of the train-evoked [Ca2+]i changes. Carbachol 62-65 carbonic anhydrase 2 Rattus norvegicus 124-127 9221776-12 1997 These effects of CCh on dendritic spike potentials and associated [Ca2+]i changes may be important in modulating synaptic integration and plasticity in these neurons. Carbachol 17-20 carbonic anhydrase 2 Rattus norvegicus 67-70 9211926-3 1997 In this report, using a detergent-free method for isolation of sarcolemmal caveolae from primary cultures of adult rat ventricular myocytes, we demonstrated that the muscarinic cholinergic agonist carbachol promotes the translocation of mAchR into low density gradient fractions containing most myocyte caveolin-3 and eNOS. Carbachol 197-206 caveolin 3 Rattus norvegicus 303-313 9211926-3 1997 In this report, using a detergent-free method for isolation of sarcolemmal caveolae from primary cultures of adult rat ventricular myocytes, we demonstrated that the muscarinic cholinergic agonist carbachol promotes the translocation of mAchR into low density gradient fractions containing most myocyte caveolin-3 and eNOS. Carbachol 197-206 nitric oxide synthase 3 Rattus norvegicus 318-322 9211799-0 1997 Effects of myosin light chain kinase inhibitors on carbachol-activated nonselective cationic current in guinea-pig gastric myocytes. Carbachol 51-60 myosin light chain kinase, smooth muscle Cavia porcellus 11-36 9211814-4 1997 The magnitudes of the peak and plateau of [Ca2+]i transients induced by carbachol (CCH, 10(-6) mol/l) were greatly enhanced by an acidic pHi and nearly abolished by an alkaline pHi. Carbachol 72-81 glucose-6-phosphate isomerase Homo sapiens 177-180 9211814-4 1997 The magnitudes of the peak and plateau of [Ca2+]i transients induced by carbachol (CCH, 10(-6) mol/l) were greatly enhanced by an acidic pHi and nearly abolished by an alkaline pHi. Carbachol 83-86 glucose-6-phosphate isomerase Homo sapiens 137-140 9211814-4 1997 The magnitudes of the peak and plateau of [Ca2+]i transients induced by carbachol (CCH, 10(-6) mol/l) were greatly enhanced by an acidic pHi and nearly abolished by an alkaline pHi. Carbachol 83-86 glucose-6-phosphate isomerase Homo sapiens 177-180 9211814-6 1997 This effect of pHi was also observed at higher CCH concentrations (10(-4 )and 10(-5) mol/l), at which the inhibitory effect of an alkaline pHi was more pronounced than the stimulatory effect of an acidic pHi. Carbachol 47-50 glucose-6-phosphate isomerase Homo sapiens 15-18 9211814-6 1997 This effect of pHi was also observed at higher CCH concentrations (10(-4 )and 10(-5) mol/l), at which the inhibitory effect of an alkaline pHi was more pronounced than the stimulatory effect of an acidic pHi. Carbachol 47-50 glucose-6-phosphate isomerase Homo sapiens 139-142 9211814-6 1997 This effect of pHi was also observed at higher CCH concentrations (10(-4 )and 10(-5) mol/l), at which the inhibitory effect of an alkaline pHi was more pronounced than the stimulatory effect of an acidic pHi. Carbachol 47-50 glucose-6-phosphate isomerase Homo sapiens 139-142 9211814-7 1997 An acidic pHi shifted the CCH concentration/response curve to the left, whereas an alkaline pHi led to a rightward shift. Carbachol 26-29 glucose-6-phosphate isomerase Homo sapiens 10-13 9211814-10 1997 The InsP3 increase induced by CCH was unaltered at an acidic pHi, but was augmented at an alkaline pHi. Carbachol 30-33 glucose-6-phosphate isomerase Homo sapiens 61-64 9211814-10 1997 The InsP3 increase induced by CCH was unaltered at an acidic pHi, but was augmented at an alkaline pHi. Carbachol 30-33 glucose-6-phosphate isomerase Homo sapiens 99-102 9207273-8 1997 IFN-gamma and IL-10 both separately reduced peak forskolin and carbachol-stimulated Isc. Carbachol 63-72 interferon gamma Homo sapiens 0-9 9252447-4 1997 Carbachol and bombesin, but not vasoactive intestinal peptide, also activated p70s6k. Carbachol 0-9 ribosomal protein S6 kinase B1 Rattus norvegicus 78-84 9207273-8 1997 IFN-gamma and IL-10 both separately reduced peak forskolin and carbachol-stimulated Isc. Carbachol 63-72 interleukin 10 Homo sapiens 14-19 9207273-11 1997 In addition, both IL-10 and IFN-gamma limit carbachol and forskolin-induced increase in Isc. Carbachol 44-53 interleukin 10 Homo sapiens 18-23 9207273-11 1997 In addition, both IL-10 and IFN-gamma limit carbachol and forskolin-induced increase in Isc. Carbachol 44-53 interferon gamma Homo sapiens 28-37 9191080-3 1997 Pre-incubation with muscarinic antagonists or the protein kinase C inhibitor GF109203X demonstrated that, in both control and ethanol-treated cells, carbachol-induced c-fos expression was mediated via muscarinic M1 receptors and to a large extent through protein kinase C. However, phorbol ester-induced c-fos expression was unaffected in ethanol-treated cells. Carbachol 149-158 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 304-309 9191080-2 1997 Four days of ethanol exposure enhanced carbachol-stimulated c-fos mRNA expression, analyzed with Northern blot, and Fos/AP-1 binding activity, measured with gel mobility super shift assay. Carbachol 39-48 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 60-65 9191080-4 1997 Acute exposure to ethanol caused a suppression of both carbachol- and phorbol ester-stimulated c-fos expression. Carbachol 55-64 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 95-100 9191080-3 1997 Pre-incubation with muscarinic antagonists or the protein kinase C inhibitor GF109203X demonstrated that, in both control and ethanol-treated cells, carbachol-induced c-fos expression was mediated via muscarinic M1 receptors and to a large extent through protein kinase C. However, phorbol ester-induced c-fos expression was unaffected in ethanol-treated cells. Carbachol 149-158 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 167-172 9176219-7 1997 IGF-I, but not GH, attenuated TPN-induced increases in tissue conductance and carbachol-stimulated ion secretion. Carbachol 78-87 insulin-like growth factor 1 Rattus norvegicus 0-5 9178925-8 1997 Carbachol, secretin, CCK-8 and prostaglandin E2 (PGE2) strongly stimulated mucin secretion, and gastrin I weakly did. Carbachol 0-9 solute carrier family 13 member 2 Rattus norvegicus 75-80 9228206-4 1997 In PRL-treated islets, the 45Ca2+ content after a 5 min incubation in the presence of G, Leu, Arg and Cch was significantly higher than the control only in islets cultured for 19 days. Carbachol 102-105 prolactin Rattus norvegicus 3-6 9131640-5 1997 Carbachol-stimulated CDP-DAG formation required trace amount of Ca2+ and the response was inhibited by NMDA at low but not high extracellular Ca2+ concentrations. Carbachol 0-9 cut-like homeobox 1 Rattus norvegicus 21-24 9131640-7 1997 The inhibition of carbachol-stimulated CDP-DAG formation was not affected by adding tetrodotoxin or cobalt chloride suggesting the inhibitory effect was not due to releasing of neurotransmitters. Carbachol 18-27 cut-like homeobox 1 Rattus norvegicus 39-42 9144240-7 1997 PKC-stimulated phosphorylation occurs predominantly on serine residues and can be induced either by direct stimulation of PKC with phorbol-12,13-dibutyrate or by activation of the m1 acetylcholine receptor-signaling pathway with the muscarinic agonist carbachol. Carbachol 252-261 proline rich transmembrane protein 2 Homo sapiens 0-3 9131640-9 1997 When cortical slices were preincubated with ligands and lithium to allow the build up of CDP-DAG, carbachol stimulated the incorporation of [3H]PtdIns. Carbachol 98-107 cut-like homeobox 1 Rattus norvegicus 89-92 9142920-9 1997 Moreover, in CFTR(-/-) mice, both forskolin- and carbachol-stimulated peak HCO3- secretions were fourfold less compared with those in CFTR(+/+) littermates (3.7 +/- 0.2 vs. 15.6 +/- 2.1 and 4.7 +/- 0.3 vs. 14.2 +/- 2.5 micromol x cm(-1) x h(-1), respectively; P < 0.01). Carbachol 49-58 cystic fibrosis transmembrane conductance regulator Mus musculus 13-17 9142910-5 1997 A-23187, bombesin, substance P, and carbachol increased the MP mRNA level. Carbachol 36-45 serine peptidase inhibitor, Kazal type 1 Rattus norvegicus 60-62 9151658-8 1997 PKCepsilon was the most responsive to PdBu reaching almost maximal translocation at a PdBu concentration as low as 10(-9) M. The cholinergic agonist, carbachol (10(-5) and 10(-3) M), induced translocation which was transient for PKCdelta, and -mu, but persisted for 10 min for PKCepsilon. Carbachol 150-159 protein kinase C epsilon Homo sapiens 0-10 9105698-7 1997 In contrast, in bronchi contracted with carbachol, 1 microM, the concentration-response curve to SCA 40 was monophasic and yielded a pD2 of 6.31 +/- 0.29. Carbachol 40-49 coiled-coil domain containing 88C Homo sapiens 97-103 9085978-8 1997 ET-1 (3 x 10(-8) M) induced contractions that were 76 +/- 3% of those induced by carbachol. Carbachol 81-90 endothelin 1 Homo sapiens 0-4 9085978-10 1997 Similarly, ET-2 (3 x 10(-8) M) induced contractions that were 74 +/- 5% of those induced by carbachol, and KET also reversed this response in a dose-dependent manner. Carbachol 92-101 endothelin 2 Homo sapiens 11-15 9085978-11 1997 In epithelium-denuded strips, ET-1 induced contractions that were 104 +/- 3% of those induced by carbachol, and KET still reversed this response. Carbachol 97-106 endothelin 1 Homo sapiens 30-34 9251770-0 1997 Functional evidence that the central renin-angiotensin system plays a role in the pressor response induced by central injection of carbachol. Carbachol 131-140 renin Rattus norvegicus 37-42 9105698-19 1997 In bronchi contracted with carbachol, 1 microM, the nature of the interaction between SCA 40 and the beta 2-adrenoceptor agonist, salbutamol, was synergistic. Carbachol 27-36 coiled-coil domain containing 88C Homo sapiens 86-92 9105698-19 1997 In bronchi contracted with carbachol, 1 microM, the nature of the interaction between SCA 40 and the beta 2-adrenoceptor agonist, salbutamol, was synergistic. Carbachol 27-36 adrenoceptor beta 2 Homo sapiens 101-120 9083272-3 1997 We examined the effect of the cholinergic agent carbachol on the activity of the Na-HCO3 cotransporter in primary cultures of the proximal tubule of the rabbit. Carbachol 48-57 electrogenic sodium bicarbonate cotransporter 1 Oryctolagus cuniculus 81-102 9209957-9 1997 With addition of 100 microM carbamylcholine, the dissociation was expressed as normal secretion of insulin and hypersecretion of IAPP. Carbachol 28-43 insulin Homo sapiens 99-106 9209957-9 1997 With addition of 100 microM carbamylcholine, the dissociation was expressed as normal secretion of insulin and hypersecretion of IAPP. Carbachol 28-43 islet amyloid polypeptide Homo sapiens 129-133 9173894-4 1997 This was consistent with the observation that carbachol elicited Ca2+ mobilization and PKC-dependent phosphorylation of cytosolic phospholipase A2 (cPLA2; phosphatide sn-2-acylhydrolase, EC 3.1.1.4) as measured by a decrease in electrophoretic mobility. Carbachol 46-55 phospholipase A2 group IVA Homo sapiens 120-146 9173894-4 1997 This was consistent with the observation that carbachol elicited Ca2+ mobilization and PKC-dependent phosphorylation of cytosolic phospholipase A2 (cPLA2; phosphatide sn-2-acylhydrolase, EC 3.1.1.4) as measured by a decrease in electrophoretic mobility. Carbachol 46-55 phospholipase A2 group IVA Homo sapiens 148-153 9083272-8 1997 Because carbachol activates phospholipase C and protein kinase C, we examined the effect of carbachol in the presence of the phospholipase C inhibitor, U73122, or the PKC inhibitor, calphostin C, or PKC depletion. Carbachol 8-17 LOC100009319 Oryctolagus cuniculus 28-43 9083272-9 1997 The phospholipase C inhibitor prevented both the effect of carbachol on the cotransporter and on the intracellular Ca. Carbachol 59-68 LOC100009319 Oryctolagus cuniculus 4-19 9132013-1 1997 The difference between infrared spectra of the nicotinic acetylcholine receptor (nAChR) recorded using the attenuated total reflectance technique in the presence and absence of carbamylcholine exhibits a complex pattern of positive and negative bands that provides a spectral map of the structural changes that occur in the nAChR upon agonist binding and subsequent desensitization. Carbachol 177-192 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 47-79 9094163-3 1997 From enzymatic assays, PLA2 and diacylglycerol (DAG) lipase were activated by Cch and respectively inhibited by the PLA2 inhibitors, mepacrine and aristolochic acid, and by the DAG lipase inhibitor, RHC 80267. Carbachol 78-81 phospholipase A2 group IB Rattus norvegicus 23-27 9094163-3 1997 From enzymatic assays, PLA2 and diacylglycerol (DAG) lipase were activated by Cch and respectively inhibited by the PLA2 inhibitors, mepacrine and aristolochic acid, and by the DAG lipase inhibitor, RHC 80267. Carbachol 78-81 phospholipase A2 group IB Rattus norvegicus 116-120 9094163-8 1997 RHC 80267 and the PLA2 inhibitors separately and partially suppressed Cch-stimulated amylase secretion, with an additive effect observed when the DAG lipase and the PLA2 inhibitors were combined. Carbachol 70-73 phospholipase A2 group IB Rattus norvegicus 18-22 9132013-1 1997 The difference between infrared spectra of the nicotinic acetylcholine receptor (nAChR) recorded using the attenuated total reflectance technique in the presence and absence of carbamylcholine exhibits a complex pattern of positive and negative bands that provides a spectral map of the structural changes that occur in the nAChR upon agonist binding and subsequent desensitization. Carbachol 177-192 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 81-86 9132013-1 1997 The difference between infrared spectra of the nicotinic acetylcholine receptor (nAChR) recorded using the attenuated total reflectance technique in the presence and absence of carbamylcholine exhibits a complex pattern of positive and negative bands that provides a spectral map of the structural changes that occur in the nAChR upon agonist binding and subsequent desensitization. Carbachol 177-192 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 324-329 9073150-0 1997 Effect of carbachol on vascular and luminal release of immunoreactive gastrin from isolated perfused rat duodenum. Carbachol 10-19 gastrin Rattus norvegicus 70-77 9130158-8 1997 Somatostatin (1-300 nM), carbamylcholine (0.1-1 microM) and muscarine (0.1-1 microM) each had a dose-dependent inhibitory effect, from a decrease of Ca2+ spike duration and frequency to a complete block of the GHRH-evoked action potentials. Carbachol 25-40 growth hormone releasing hormone Rattus norvegicus 210-214 9045727-6 1997 Biochemical assays and focal applications of several agonists (methoxamine, carbachol, glutamate) of membrane receptors to neurotransmitters and peptides (endothelin 1) demonstrated that their ability to trigger regenerative calcium waves depended on phospholipase C activity and inositol phosphate production. Carbachol 76-85 endothelin 1 Rattus norvegicus 155-167 9175612-5 1997 L-AP4 (IC50 = >300 microM), D-AP4 (IC50 = >100 microM) and L-SOP (IC50 = 199 +/- 6 microM) inhibited 6 microM (S)-AMPA-stimulated 57Co2+ influx, whereas L-CCG-1 (up to 10 microM), 300 microM (RS)-3,5-dihydroxyphenylglycine, 300 microM (+/-)-baclofen and 1 mM carbachol were ineffective. Carbachol 265-274 DLG associated protein 4 Homo sapiens 31-36 9196397-10 1997 Thus, 17-phenyl PGF2 alpha (1 microM) weakly contracted the ciliary muscle (4.8%), sulprostone (1 microM) the trabecular meshwork (10.1%), 11-deoxy PGE1 (1 microM) and AH13205 (10 microM) elicited relaxations in both tissue precontracted with carbachol (1 microM). Carbachol 243-252 prostaglandin F synthase 2 Bos taurus 16-20 9049150-0 1997 Differential stimulation of intestinal mucin secretion by cholera toxin and carbachol. Carbachol 76-85 LOC100508689 Homo sapiens 39-44 9049150-6 1997 Cholera toxin caused a 116-fold increase of intracellular cAMP and strongly stimulated the secretion of both preformed and newly synthesized mucin for more than 20 h. Carbachol only triggered the release of preformed mucin immediately after addition. Carbachol 167-176 LOC100508689 Homo sapiens 141-146 9038886-2 1997 The present study was designed to investigate activation of PLC-beta isoenzymes by three different PLC-activating agonists that bind to different receptor entities, i.e., cholecystokinin octapeptide (CCK-8), bombesin, and carbachol in rat pancreatic acinar membranes. Carbachol 222-231 phospholipase C, gamma 1 Rattus norvegicus 60-63 9049150-6 1997 Cholera toxin caused a 116-fold increase of intracellular cAMP and strongly stimulated the secretion of both preformed and newly synthesized mucin for more than 20 h. Carbachol only triggered the release of preformed mucin immediately after addition. Carbachol 167-176 LOC100508689 Homo sapiens 217-222 9100288-6 1997 Gastrin treatment (5 micrograms.kg-1.h-1) of CR rats restored the gastric acid responses to both histamine and carbachol. Carbachol 111-120 gastrin Rattus norvegicus 0-7 9100288-7 1997 These results suggest that CR can selectively decrease the gastric acid responses to both histamine and carbachol by depletion of the endogenous tissue stores of gastrin. Carbachol 104-113 gastrin Rattus norvegicus 162-169 9124375-3 1997 Myelin basic protein kinase activities corresponding to ERK1 and ERK2 immunoreactive proteins were activated twofold above the basal level within 5 min by 1 microM carbachol. Carbachol 164-173 mitogen-activated protein kinase 3 Mus musculus 56-60 9124375-3 1997 Myelin basic protein kinase activities corresponding to ERK1 and ERK2 immunoreactive proteins were activated twofold above the basal level within 5 min by 1 microM carbachol. Carbachol 164-173 mitogen-activated protein kinase 1 Mus musculus 65-69 9124375-6 1997 Carbachol stimulation increased caldesmon phosphorylation in intact muscle, and purified caldesmon was a substrate for activated murine ERK2 MAP kinase. Carbachol 0-9 mitogen-activated protein kinase 1 Mus musculus 136-140 9100260-0 1997 Involvement of M2 receptor in an enhancement of long-term potentiation by carbachol in Schaffer collateral-CA1 synapses of hippocampal slices. Carbachol 74-83 carbonic anhydrase 1 Cavia porcellus 107-110 9100260-1 1997 We examined effects of carbachol (CCh), muscarinic receptor agonist, on long-term potentiation (LTP) of field excitatory postsynaptic potential (fEPSP) at Schaffer collateral-CA1 synapse of guinea pig hippocampal slices using extracellular recording technique. Carbachol 23-32 carbonic anhydrase 1 Cavia porcellus 175-178 9100260-1 1997 We examined effects of carbachol (CCh), muscarinic receptor agonist, on long-term potentiation (LTP) of field excitatory postsynaptic potential (fEPSP) at Schaffer collateral-CA1 synapse of guinea pig hippocampal slices using extracellular recording technique. Carbachol 34-37 carbonic anhydrase 1 Cavia porcellus 175-178 9100260-6 1997 These results suggest that the induction of LTP at Schaffer collateral-CA1 synapse was enhanced through the activation of M2 receptors by CCh at a lower concentration. Carbachol 138-141 carbonic anhydrase 1 Cavia porcellus 71-74 9080376-11 1997 Both carbachol and PGF2 alpha potentiated phosphorylation of MLC20 in depolarized tissues. Carbachol 5-14 myosin light chain 12B Rattus norvegicus 61-66 9031749-6 1997 However, when either NPY (300 pM-1 microM) or SRIF (300 pM-1 microM) was applied in the presence of the cholinoceptor agonist carbachol (1 microM or 100 microM) they evoked an elevation of [Ca2+]i above that caused by carbachol alone. Carbachol 126-135 neuropeptide Y Homo sapiens 21-24 9031749-6 1997 However, when either NPY (300 pM-1 microM) or SRIF (300 pM-1 microM) was applied in the presence of the cholinoceptor agonist carbachol (1 microM or 100 microM) they evoked an elevation of [Ca2+]i above that caused by carbachol alone. Carbachol 218-227 neuropeptide Y Homo sapiens 21-24 9031749-9 1997 In the presence of 1 microM or 100 microM carbachol NPY elevated [Ca2+]i with a pEC50 of 7.80 and 7.86 respectively. Carbachol 42-51 neuropeptide Y Homo sapiens 52-55 9031749-19 1997 Block of carbachol activation of muscarinic receptors with atropine (1 microM) abolished the elevation of [Ca2+]i by the SRIF and NPY. Carbachol 9-18 neuropeptide Y Homo sapiens 130-133 9000425-7 1997 Refilling of internal Ca2+ store(s) in carbachol-treated cells (incubation with Ca2++atropine) induced complete inhibition of divalent cation influx, which was not prevented by treatment with protein kinase inhibitors. Carbachol 39-48 KIT proto-oncogene receptor tyrosine kinase Rattus norvegicus 192-206 8995260-7 1997 The predominant role of NHE-1 in carbachol-induced Na+/H+ exchange was established pharmacologically using HOE694, an inhibitor with differential potency toward the individual isoforms. Carbachol 33-42 solute carrier family 9 member A1 Homo sapiens 24-29 8978749-3 1997 The potentiation effect of arginine on carbachol-induced calcium mobilization was mimicked by either 8-bromo cyclic GMP or sodium nitroprusside. Carbachol 39-48 5'-nucleotidase, cytosolic II Homo sapiens 116-119 9038869-7 1997 Both phenylephrine and octreotide decreased the release of motilin stimulated by carbachol in a jejunal segment pretreated and denervated with tetrodotoxin. Carbachol 81-90 motilin Canis lupus familiaris 59-66 9038886-6 1997 The order of sensitivity toward inhibition by anti-PLC-beta 1 antibody was CCK-8 > bombesin > carbachol. Carbachol 100-109 phospholipase C beta 1 Rattus norvegicus 51-61 9038886-7 1997 An opposite order of sensitivity was found for inhibition of PLC activity by anti-PLC-beta 3 antibody (carbachol > bombesin > CCK-8). Carbachol 103-112 phospholipase C, gamma 1 Rattus norvegicus 61-64 9038886-7 1997 An opposite order of sensitivity was found for inhibition of PLC activity by anti-PLC-beta 3 antibody (carbachol > bombesin > CCK-8). Carbachol 103-112 phospholipase C beta 3 Rattus norvegicus 82-92 9038886-7 1997 An opposite order of sensitivity was found for inhibition of PLC activity by anti-PLC-beta 3 antibody (carbachol > bombesin > CCK-8). Carbachol 103-112 cholecystokinin Rattus norvegicus 132-135 9038886-10 1997 In conclusion, the data of the present study indicate that CCK-8 and carbachol activate PLC-beta 1 and PLC-beta 3, respectively, whereas bombesin activates both PLC-beta 1 and PLC-beta 3. Carbachol 69-78 phospholipase C beta 1 Rattus norvegicus 88-98 9038886-10 1997 In conclusion, the data of the present study indicate that CCK-8 and carbachol activate PLC-beta 1 and PLC-beta 3, respectively, whereas bombesin activates both PLC-beta 1 and PLC-beta 3. Carbachol 69-78 phospholipase C beta 3 Rattus norvegicus 103-113 8978749-6 1997 It is suggested that the NO production and subsequent cyclic GMP elevation induced by arginine are responsible for the potentiation of carbachol-induced Ca2+ increase. Carbachol 135-144 5'-nucleotidase, cytosolic II Homo sapiens 61-64 9121353-3 1997 Treatment of chick embryos in ovo with carbachol results in decreased levels of mRNA encoding the potassium channel subunits GIRK1 and GIRK4 as well as the m2 receptor. Carbachol 39-48 Potassium inwardly rectifying channel subfamily J member 3 Gallus gallus 125-130 9121353-3 1997 Treatment of chick embryos in ovo with carbachol results in decreased levels of mRNA encoding the potassium channel subunits GIRK1 and GIRK4 as well as the m2 receptor. Carbachol 39-48 Potassium inwardly rectifying channel subfamily J member 5 Gallus gallus 135-140 9121362-5 1997 Only the PC3 cells responded to carbachol with an increase in turnover of polyphosphoinositides, and none of the cell lines responded with effects on cAMP metabolism. Carbachol 32-41 proprotein convertase subtilisin/kexin type 1 Homo sapiens 9-12 9121362-7 1997 Mitogen activated protein kinase (ERK) activity was seen in response to carbachol in PC3 and DU145 but not LnCaP cells. Carbachol 72-81 mitogen-activated protein kinase 1 Homo sapiens 34-37 9121362-7 1997 Mitogen activated protein kinase (ERK) activity was seen in response to carbachol in PC3 and DU145 but not LnCaP cells. Carbachol 72-81 proprotein convertase subtilisin/kexin type 1 Homo sapiens 85-88 9247321-1 1997 In rat olfactory bulb membranes, the stimulation of adenylyl cyclase by the cholinergic agonist carbachol (CCh) was markedly inhibited by heparin at concentrations (0.3-10 microM) that had smaller or no effects on the enzyme stimulations elicited by vasoactive intestinal peptide, pituitary adenylate cyclase activating polypeptide (PACAP), 1-isoproterenol and corticotropin releasing hormone. Carbachol 96-105 adenylate cyclase activating polypeptide 1 Rattus norvegicus 333-338 9503254-3 1997 At high concentrations, the 5-HT1A agonist 8-OH-DPAT attenuated the carbachol-induced stimulation of inositol phosphates (InsPs) production, but this was not blocked by the presence of 5-HT1A antagonists. Carbachol 68-77 5-hydroxytryptamine receptor 1A Oryctolagus cuniculus 28-34 9247321-1 1997 In rat olfactory bulb membranes, the stimulation of adenylyl cyclase by the cholinergic agonist carbachol (CCh) was markedly inhibited by heparin at concentrations (0.3-10 microM) that had smaller or no effects on the enzyme stimulations elicited by vasoactive intestinal peptide, pituitary adenylate cyclase activating polypeptide (PACAP), 1-isoproterenol and corticotropin releasing hormone. Carbachol 107-110 adenylate cyclase activating polypeptide 1 Rattus norvegicus 333-338 9213360-4 1997 Only 4-DAMP inhibited carbachol-induced motilin release in perifused duodenal mucosal cells. Carbachol 22-31 motilin Canis lupus familiaris 40-47 8942735-6 1996 Guanylin-stimulated HCO3- secretion was independent of luminal Cl-, inhibited by the Cl- channel blocker 5-nitro-2-(3-phenylpropylamino)-benzoate, and additive to the HCO3- secretory rate stimulated by glucagon and carbachol but not by the tested adenosine 3",5"-cyclic monophosphate (cAMP)-dependent agonists. Carbachol 215-224 guanylate cyclase activator 2A Rattus norvegicus 0-8 9037533-0 1996 Carbachol stimulates c-fos expression and proliferation in oligodendrocyte progenitors. Carbachol 0-9 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 21-26 9037533-2 1996 Using Northern blot analysis we showed that carbachol caused a time and concentration-dependent increase in c-fos mRNA. Carbachol 44-53 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 108-113 9037533-5 1996 Down-regulation of PKC by overnight pre-treatment with 12-O-tetradecanoylphorbol 13-acetate (TPA) blocked only the phorbol ester-stimulated c-fos accumulation while no effect was observed in the carbachol-induced response. Carbachol 195-204 proline rich transmembrane protein 2 Homo sapiens 19-22 9037533-6 1996 These results suggested that carbachol stimulated an H-7 sensitive PKC pathway which may be different than that activated by TPA. Carbachol 29-38 proline rich transmembrane protein 2 Homo sapiens 67-70 9037533-8 1996 Induction of c-fos mRNA by carbachol was dependent on both influx of extracellular Ca2+ and release from intracellular stores, as both EDTA and BAPTA blocked the response. Carbachol 27-36 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 13-18 8942723-6 1996 RESULTS: In vitro, c-motilin release was stimulated by carbachol but not by p-motilin, and the carbachol-induced c-motilin release was inhibited by atropine. Carbachol 55-64 motilin Canis lupus familiaris 21-28 8942723-6 1996 RESULTS: In vitro, c-motilin release was stimulated by carbachol but not by p-motilin, and the carbachol-induced c-motilin release was inhibited by atropine. Carbachol 95-104 motilin Canis lupus familiaris 21-28 9008642-4 1997 RESULTS: Pharmacologic agents [Arg8]-vasopressin, carbachol, adenosine triphosphate, substance P, fetal calf serum, and histamine all elicited, to some extent, a biphasic [Ca2+]i transient by releasing Ca2+ from InsP3-sensitive Ca2+ stores. Carbachol 50-59 carbonic anhydrase 2 Rattus norvegicus 172-175 9008642-4 1997 RESULTS: Pharmacologic agents [Arg8]-vasopressin, carbachol, adenosine triphosphate, substance P, fetal calf serum, and histamine all elicited, to some extent, a biphasic [Ca2+]i transient by releasing Ca2+ from InsP3-sensitive Ca2+ stores. Carbachol 50-59 carbonic anhydrase 2 Rattus norvegicus 202-205 9008642-4 1997 RESULTS: Pharmacologic agents [Arg8]-vasopressin, carbachol, adenosine triphosphate, substance P, fetal calf serum, and histamine all elicited, to some extent, a biphasic [Ca2+]i transient by releasing Ca2+ from InsP3-sensitive Ca2+ stores. Carbachol 50-59 carbonic anhydrase 2 Rattus norvegicus 202-205 9051814-3 1996 We report that expression of PS-1 mutation L286V in cultured PC12 cells exaggerates Ca2+ responses to agonists (carbachol and bradykinin) that induce Ca2+ release from ER. Carbachol 112-121 presenilin 1 Rattus norvegicus 29-33 9117394-9 1996 Finally, the release of [3H]GABA evoked by the combined application of NMDA and carbachol (a treatment known to markedly stimulate arachidonic acid formation) was reduced by inhibitors of phospholipase A2 further indicating that endogenously formed arachidonic acid significantly facilitates the release of GABA in the striatum. Carbachol 80-89 phospholipase A2 group IB Rattus norvegicus 188-204 8922737-4 1996 The ETB receptor-selective agonist, sarafotoxin S6c (30 nM) induced large transient contractions (118 +/- 5% Cmax, n = 13; where Cmax is the contraction induced by 10 microM carbachol) of isolated tracheal segments from control mice. Carbachol 174-183 endothelin receptor type B Mus musculus 4-7 9019738-12 1996 An acid pHi attenuated, and an alkaline pHi enhanced, carbachol- and thapsigargin-induced [Ca2+]i influx. Carbachol 54-63 glucose-6-phosphate isomerase Homo sapiens 8-11 9019738-12 1996 An acid pHi attenuated, and an alkaline pHi enhanced, carbachol- and thapsigargin-induced [Ca2+]i influx. Carbachol 54-63 glucose-6-phosphate isomerase Homo sapiens 40-43 8910218-2 1996 The aim of this study was to determine whether the low molecular mass GTPase RhoA or related proteins are involved in carbachol- and high-K(+)-induced contractions in intact intestinal smooth muscle as well as the carbachol-induced increase in Ca2+ sensitivity of the myofilaments in permeabilized preparations. Carbachol 118-127 transforming protein RhoA Cavia porcellus 77-81 8910218-2 1996 The aim of this study was to determine whether the low molecular mass GTPase RhoA or related proteins are involved in carbachol- and high-K(+)-induced contractions in intact intestinal smooth muscle as well as the carbachol-induced increase in Ca2+ sensitivity of the myofilaments in permeabilized preparations. Carbachol 214-223 transforming protein RhoA Cavia porcellus 77-81 8910218-4 1996 The carbachol-induced increase in the Ca2+ sensitivity of force production in beta-escin-permeabilized intestinal smooth muscle was enhanced in preparations that were loaded with the constitutively active mutant of RhoA, Val14RhoA, and was inhibited by exoenzyme C3 from Clostridium botulinum, which ADP-ribosylates and inactivates small GTPases of the Rho family. Carbachol 4-13 transforming protein RhoA Cavia porcellus 215-219 8900438-9 1996 Other cellular effects of CAI included an attenuation of the elevation of intracellular free calcium in response to bombesin and carbachol in PC3 cells and a marked dose-dependent inhibition of prostate-specific antigen secretion in LNCaP cell cultures. Carbachol 129-138 chromobox 8 Homo sapiens 142-145 8897815-3 1996 Stimulation of insulin release evoked by glucose, phospholipase C activation with carbachol, and protein kinase C activation with phorbol ester were obtained by SIN-1, whereas the response to adenylyl cyclase activation or K(+)-induced depolarization was not affected. Carbachol 82-91 insulin Homo sapiens 15-22 8897815-3 1996 Stimulation of insulin release evoked by glucose, phospholipase C activation with carbachol, and protein kinase C activation with phorbol ester were obtained by SIN-1, whereas the response to adenylyl cyclase activation or K(+)-induced depolarization was not affected. Carbachol 82-91 MAPK associated protein 1 Homo sapiens 161-166 8897836-3 1996 In contrast, at higher supermaximal concentrations of agonists (> 300 pM CCK, > 1 microM CCh), which induce a large "peak-and-plateau" intracellular Ca2+ signal, all cells in the acinus appeared to increase Ca2+ concentration ([Ca2+]) in synchrony. Carbachol 95-98 cholecystokinin Rattus norvegicus 76-79 8897883-13 1996 Carbachol, but not EGF or TPA, also activated an unidentified 70-kDa protein kinase as detected with in-gel myelin basic protein (MBP) kinase renaturation assays. Carbachol 0-9 myelin basic protein Oryctolagus cuniculus 108-128 8897883-13 1996 Carbachol, but not EGF or TPA, also activated an unidentified 70-kDa protein kinase as detected with in-gel myelin basic protein (MBP) kinase renaturation assays. Carbachol 0-9 myelin basic protein Oryctolagus cuniculus 130-133 8831588-7 1996 IL-10 reversed, or markedly attenuated, forskolin- and carbachol-induced net chloride secretion. Carbachol 55-64 interleukin 10 Rattus norvegicus 0-5 8815874-4 1996 H2O2 and carbachol individually induced dose- and time-dependent increases in AP-1 and NF kappa B. Carbachol 9-18 nuclear factor kappa B subunit 1 Homo sapiens 87-97 8815874-6 1996 Carbachol"s stimulation of NF kappa B was not inhibited except with a high concentration (300 microM) of H2O2, which was associated with impaired activation of protein kinase C. Lower concentrations of H2O2 (30-300 microM) inhibited carbachol-induced [3H]phosphoinositide hydrolysis, and this inhibition correlated (r = 0.95) with the inhibition of carbachol-induced AP-1. Carbachol 0-9 nuclear factor kappa B subunit 1 Homo sapiens 27-37 8892103-8 1996 Rp-adenosine-3",5"-cyclic monophosphothioate (Rp-cAMPS; 25 microM), a potent inhibitor of cAMP-dependent protein kinase A (PKA), alone decreased the amplitude of EPSP below baseline values and mimicked the inhibitory effect of DA on the carbachol-induced depression of the EPSP amplitude. Carbachol 237-246 calmodulin 2, pseudogene 1 Rattus norvegicus 49-54 8863852-6 1996 Furthermore, carbachol pretreatment also enhanced, by approximately 2.5-fold, stimulation of PLC activity on direct activation of G proteins by AIF4- and guanosine-5"-O-(3-thio)-triphosphate in intact and permeabilized cells, respectively. Carbachol 13-22 itchy E3 ubiquitin protein ligase Homo sapiens 144-148 8923487-3 1996 Acute exposure to SP inhibits carbamylcholine- or nicotine-stimulated function measured using 86Rb+ efflux assays of human ganglionic (alpha 3 beta 4) nAChR expressed in SH-SY5Y neuroblastoma cells (IC50 approximately 2.3 microM) or of human muscle-type (alpha 1 beta 1 gamma delta) nAChR expressed in TE671/RD clonal cells (IC50 approximately 21 microM). Carbachol 30-45 cholinergic receptor nicotinic alpha 4 subunit Homo sapiens 151-156 8810600-12 1996 Inhaled tryptase (500 ng) also caused airway hyperresponsiveness to aerosolized carbachol 2 h after tryptase challenge. Carbachol 80-89 tryptase beta-2 Ovis aries 8-16 8810600-12 1996 Inhaled tryptase (500 ng) also caused airway hyperresponsiveness to aerosolized carbachol 2 h after tryptase challenge. Carbachol 80-89 tryptase beta-2 Ovis aries 100-108 8843722-2 1996 Basolateral EGF inhibited Cl- secretion induced by carbachol or thapsigargin, without blocking the rise in intracellular Ca2+. Carbachol 51-60 epidermal growth factor Homo sapiens 12-15 8843732-5 1996 However, stimulation of mouse islets with the protein kinase C (PKC) activator tetradecanoyl phorbol acetate (TPA) or the muscarinic agonist carbachol, which significantly activates an isozyme of PLC distinct from that activated by high glucose, induces a rising and sustained second-phase insulin secretory response. Carbachol 141-150 insulin Homo sapiens 290-297 8809056-5 1996 In insulin-secreting beta TC6-F7 cells, the secretagogues glucose and carbachol (at maximally effective concentrations of 15 mM and 0.5 mM respectively) augmented fluid-phase pinocytosis 1.65-fold over the basal rate. Carbachol 70-79 insulin Cricetulus griseus 3-10 8809065-7 1996 When neuronally differentiated PC12 cells were stimulated with carbamylcholine or potassium, sPLA2 was released into the medium and reached a maximal approximately 40% release by 15 min. Carbachol 63-78 phospholipase A2 group IIA Rattus norvegicus 93-98 8809065-8 1996 Inhibitors specific to type II sPLA2 suppressed catecholamine secretion by PC12 cells which had been activated by carbamylcholine. Carbachol 114-129 phospholipase A2 group IIA Rattus norvegicus 31-36 8877593-0 1996 Evidence of systemic neuropeptide Y release after carbachol administration into the posterior hypothalamic nucleus. Carbachol 50-59 neuropeptide Y Rattus norvegicus 21-35 8858914-2 1996 Carbachol stimulated luciferase expression in cells transfected with a rat phenylethanolamine N-methyltransferase promoter-luciferase reporter gene construct and also elevated Egr-1 mRNA levels in untransfected cells. Carbachol 0-9 phenylethanolamine-N-methyltransferase Rattus norvegicus 75-113 8858914-2 1996 Carbachol stimulated luciferase expression in cells transfected with a rat phenylethanolamine N-methyltransferase promoter-luciferase reporter gene construct and also elevated Egr-1 mRNA levels in untransfected cells. Carbachol 0-9 early growth response 1 Rattus norvegicus 176-181 8858914-3 1996 Maximum induction of Egr-1 mRNA by carbachol was rapid (0.5 h), whereas by comparison, peak luciferase activity was delayed (6 h). Carbachol 35-44 early growth response 1 Rattus norvegicus 21-26 8858914-4 1996 In addition, carbachol stimulation of both luciferase and Egr-1 mRNA expression could be completely inhibited by atropine but not hexamethonium. Carbachol 13-22 early growth response 1 Rattus norvegicus 58-63 8858914-7 1996 These results suggest that carbachol activates the phenylethanolamine N-methyltransferase promotor through stimulation of Egr-1 expression, and are consistent with the potential involvement of Egr-1 in the cholinergic activation of the phenylethanolamine N-methyltransferase gene. Carbachol 27-36 phenylethanolamine-N-methyltransferase Rattus norvegicus 51-89 8858914-7 1996 These results suggest that carbachol activates the phenylethanolamine N-methyltransferase promotor through stimulation of Egr-1 expression, and are consistent with the potential involvement of Egr-1 in the cholinergic activation of the phenylethanolamine N-methyltransferase gene. Carbachol 27-36 early growth response 1 Rattus norvegicus 122-127 8858914-7 1996 These results suggest that carbachol activates the phenylethanolamine N-methyltransferase promotor through stimulation of Egr-1 expression, and are consistent with the potential involvement of Egr-1 in the cholinergic activation of the phenylethanolamine N-methyltransferase gene. Carbachol 27-36 early growth response 1 Rattus norvegicus 193-198 8858914-7 1996 These results suggest that carbachol activates the phenylethanolamine N-methyltransferase promotor through stimulation of Egr-1 expression, and are consistent with the potential involvement of Egr-1 in the cholinergic activation of the phenylethanolamine N-methyltransferase gene. Carbachol 27-36 phenylethanolamine-N-methyltransferase Rattus norvegicus 236-274 8906568-2 1996 Depletion of extracellular Ca2+ abolished the Cch-mediated phospholipase D (PLD) activation, indicating the requirement of Ca2+ influx. Carbachol 46-49 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 76-79 8718877-8 1996 Photolysis of acetylcholinesterase complexed with the 2-nitrobenzyl derivative of carbamylcholine led to time-dependent inactivation, resulting from carbamylation of acetylcholinesterase, which could be reversed upon dilution, due to decarbamylation. Carbachol 82-97 acetylcholinesterase (Cartwright blood group) Homo sapiens 14-34 8718877-8 1996 Photolysis of acetylcholinesterase complexed with the 2-nitrobenzyl derivative of carbamylcholine led to time-dependent inactivation, resulting from carbamylation of acetylcholinesterase, which could be reversed upon dilution, due to decarbamylation. Carbachol 82-97 acetylcholinesterase (Cartwright blood group) Homo sapiens 166-186 8770076-2 1996 Perfusion of hearts with carbachol and isoproterenol concurrently for 1.5 min prevented the increase in left ventricular pressure (LVP) found with isoproterenol alone, although isoproterenol-induced changes in total and particulate cAMP levels and soluble and particulate PKA activity were unaffected. Carbachol 25-34 cathelicidin antimicrobial peptide Rattus norvegicus 232-236 8770076-3 1996 However, perfusion of hearts with carbachol for 1 min then with isoproterenol and carbachol for 1.5 min abolished the isoproterenol-induced increase in LVP and in total and particulate cAMP levels, although changes in total and particulate PKA activity were only partially attenuated. Carbachol 34-43 cathelicidin antimicrobial peptide Rattus norvegicus 185-189 8770076-3 1996 However, perfusion of hearts with carbachol for 1 min then with isoproterenol and carbachol for 1.5 min abolished the isoproterenol-induced increase in LVP and in total and particulate cAMP levels, although changes in total and particulate PKA activity were only partially attenuated. Carbachol 82-91 cathelicidin antimicrobial peptide Rattus norvegicus 185-189 21153096-4 1996 From enzymatic assays, phospholipase A(2) and diacylglycerol lipase were activated by carbamylcholine and these activations were inhibited by the phospholipase A(2) inhibitors, mepacrine and aristolochic acid, and by the diacylglycerol lipase inhibitor RHC 80267. Carbachol 86-101 phospholipase A2 group IB Rattus norvegicus 23-67 8856671-8 1996 Agents that induce translocation of endogenous FGF-2 to the nucleus (forskolin, carbachol, or angiotensin II) increased the intranuclear accumulation of FGFR1. Carbachol 80-89 fibroblast growth factor 2 Bos taurus 47-52 8856671-8 1996 Agents that induce translocation of endogenous FGF-2 to the nucleus (forskolin, carbachol, or angiotensin II) increased the intranuclear accumulation of FGFR1. Carbachol 80-89 fibroblast growth factor receptor 1 Bos taurus 153-158 8692890-6 1996 Carbachol-induced accumulation of inositol phosphates (IP, IP2, IP3, and IP4) was decreased and calcium imaging studies revealed that carbachol-induced release of calcium was severely impaired in neurons pretreated with Abeta. Carbachol 0-9 amyloid beta precursor protein Rattus norvegicus 220-225 8692890-6 1996 Carbachol-induced accumulation of inositol phosphates (IP, IP2, IP3, and IP4) was decreased and calcium imaging studies revealed that carbachol-induced release of calcium was severely impaired in neurons pretreated with Abeta. Carbachol 134-143 amyloid beta precursor protein Rattus norvegicus 220-225 8692890-8 1996 The effects of Abeta on carbachol-induced GTPase activity and calcium release were attenuated by antioxidants, implicating free radicals in the mechanism whereby Abeta induced uncoupling of muscarinic receptors. Carbachol 24-33 amyloid beta precursor protein Rattus norvegicus 15-20 8692890-8 1996 The effects of Abeta on carbachol-induced GTPase activity and calcium release were attenuated by antioxidants, implicating free radicals in the mechanism whereby Abeta induced uncoupling of muscarinic receptors. Carbachol 24-33 amyloid beta precursor protein Rattus norvegicus 162-167 8670170-10 1996 Carbachol or PDBu induced cytosol to membrane translocation of PKC alpha. Carbachol 0-9 protein kinase C alpha Homo sapiens 63-72 8660365-1 1996 Three sialagogues, isoproterenol (IPR), carbachol, and methoxamine, caused induction of ornithine decarboxylase (ODC) in cultured rat parotid explants. Carbachol 40-49 ornithine decarboxylase 1 Rattus norvegicus 88-111 8660365-1 1996 Three sialagogues, isoproterenol (IPR), carbachol, and methoxamine, caused induction of ornithine decarboxylase (ODC) in cultured rat parotid explants. Carbachol 40-49 ornithine decarboxylase 1 Rattus norvegicus 113-116 8762078-7 1996 VIP was a more potent relaxant in tissues that were contracted with carbachol than those contracted with an equi-effective depolarizing concentration of K+. Carbachol 68-77 VIP peptides Cavia porcellus 0-3 8670764-6 1996 Supernatants of unstimulated and carbachol-stimulated human lacrimal gland explant cultures were evaluated for secretion of TGF-beta1 and TGF-beta2 by ELISA: RESULTS: TGF-beta1 and TGF-beta2 mRNA expression was found in all human and rabbit lacrimal gland specimens by RT-PCR. Carbachol 33-42 transforming growth factor beta 1 Homo sapiens 167-176 8670764-10 1996 TGF-beta1 was detected in supernatants of human lacrimal gland explants, and the concentration of TGF-beta1 increased by an average of 280% after carbachol-stimulation (p = 0.004). Carbachol 146-155 transforming growth factor beta 1 Homo sapiens 98-107 8964409-7 1996 Gastrin cells respond to both gastrin-releasing peptide and carbachol but not to cholecystokinin-receptor agonists. Carbachol 60-69 gastrin Rattus norvegicus 0-7 8806932-7 1996 Similarly, for carbachol, the responses were significantly lower in the alpha-1-antitrypsin deficiency group (P = 0.001; n = 10) and in specimens resected for carcinoma (P = 0.001; n = 6) than in the nondiseased group (n = 9). Carbachol 15-24 serpin family A member 1 Homo sapiens 72-91 8717044-3 1996 In NIH-3T3 cells expressing subtype 1 human muscarinic receptors (hm1), the agonist carbachol selectively increased the specific activity and phosphorylation state of epitope-tagged Ras-GRF. Carbachol 84-93 cholinergic receptor muscarinic 1 Homo sapiens 66-69 8717044-6 1996 In COS-7 cells, cotransfection of hm1 or hm2 receptors with Ras-GRF conferred carbachol-dependent increases in exchange-factor activity, whereas cotransfection with G-protein beta gamma subunits caused a constitutive activation that was sensitive to PP1. Carbachol 78-87 cholinergic receptor muscarinic 1 Homo sapiens 34-37 8679222-3 1996 Pretreatment of human TSMC with TNF alpha potentiated cytosolic calcium [(Ca2+)i] transients evoked by carbachol. Carbachol 103-112 tumor necrosis factor Homo sapiens 32-41 8679222-4 1996 In a similar manner, selective TNF alpha-p55 receptor agonists such as htr-9, an activating monoclonal antibody, or a recombinant TNF-p55 (rTNF-p55), which specifically activates the TNF alpha-p55 receptor but not the TNF alpha-p75 receptor, also augmented [Ca2+]i transients evoked by carbachol. Carbachol 286-295 tumor necrosis factor Homo sapiens 31-40 8679222-4 1996 In a similar manner, selective TNF alpha-p55 receptor agonists such as htr-9, an activating monoclonal antibody, or a recombinant TNF-p55 (rTNF-p55), which specifically activates the TNF alpha-p55 receptor but not the TNF alpha-p75 receptor, also augmented [Ca2+]i transients evoked by carbachol. Carbachol 286-295 tumor necrosis factor Rattus norvegicus 31-34 8679222-4 1996 In a similar manner, selective TNF alpha-p55 receptor agonists such as htr-9, an activating monoclonal antibody, or a recombinant TNF-p55 (rTNF-p55), which specifically activates the TNF alpha-p55 receptor but not the TNF alpha-p75 receptor, also augmented [Ca2+]i transients evoked by carbachol. Carbachol 286-295 tumor necrosis factor Rattus norvegicus 139-143 8679222-4 1996 In a similar manner, selective TNF alpha-p55 receptor agonists such as htr-9, an activating monoclonal antibody, or a recombinant TNF-p55 (rTNF-p55), which specifically activates the TNF alpha-p55 receptor but not the TNF alpha-p75 receptor, also augmented [Ca2+]i transients evoked by carbachol. Carbachol 286-295 tumor necrosis factor Homo sapiens 183-192 8679222-4 1996 In a similar manner, selective TNF alpha-p55 receptor agonists such as htr-9, an activating monoclonal antibody, or a recombinant TNF-p55 (rTNF-p55), which specifically activates the TNF alpha-p55 receptor but not the TNF alpha-p75 receptor, also augmented [Ca2+]i transients evoked by carbachol. Carbachol 286-295 tumor necrosis factor Homo sapiens 183-192 9807062-2 1996 We found that corticosterone, administered to adrenalectomized rats in vivo, dose-dependently modulates carbachol responsiveness of CA1 hippocampal neurons, recorded subsequently in vitro. Carbachol 104-113 carbonic anhydrase 1 Rattus norvegicus 132-135 9807062-3 1996 Thus, the carbachol (3 microM) induced membrane depolarization in CA1 neurons was relatively large in hippocampal slices where either (almost) no corticosteroid receptors were activated (0-1 microg corticosterone/100g body weight) or where both MRs and GRs were occupied by high corticosterone doses (100-1000 microg/100g). Carbachol 10-19 carbonic anhydrase 1 Rattus norvegicus 66-69 8761858-6 1996 In contrast, the increase in MAP evoked by 5.5 and 13.2 nmol CCh could be attenuated by prazosin, yohimbine, or D[(CH2)5Tyr(Me)]AVP (AVPX, a V 1-vasopressin receptor blocker), and completely blocked by the combination of prazosin and AVPX. Carbachol 61-64 arginine vasopressin Rattus norvegicus 145-156 8632160-2 1996 The PKC inhibitor Ro 31-7549 inhibited carbachol-evoked NA release (IC(50) 0.6 microM) but not 100 mM (K+)-evoked release. Carbachol 39-48 protein kinase C alpha Homo sapiens 4-7 8632160-7 1996 The PKC inhibitor Go-6976 (2 microM), which has been shown to inhibit selectively PKC-alpha and beta in vitro, also inhibited the TPA enhancement of carbachol- and (K+)-evoked NA release by > 50%. Carbachol 149-158 protein kinase C alpha Homo sapiens 4-7 8632160-8 1996 These data suggest that in SH-SY5Y cells, the ability of TPA to enhance carbachol- and (K+)-evoked NA secretion is due to activation of PKC-alpha. Carbachol 72-81 protein kinase C alpha Homo sapiens 136-145 8877593-5 1996 Benextramine noncompetitively inhibited the pressor response to intravenous injection of NPY and the increase in MAP evoked by CCh microinjection into adrenergic and V1-vasopressin receptor-blocked rats, whereas benextramine competitively inhibited the pressor response to angiotensin II (AII). Carbachol 127-130 angiotensinogen Rattus norvegicus 273-287 8877593-5 1996 Benextramine noncompetitively inhibited the pressor response to intravenous injection of NPY and the increase in MAP evoked by CCh microinjection into adrenergic and V1-vasopressin receptor-blocked rats, whereas benextramine competitively inhibited the pressor response to angiotensin II (AII). Carbachol 127-130 angiotensinogen Rattus norvegicus 289-292 8877593-8 1996 The similarity in the antagonism of the increase in MAP evoked by intravenous NPY injection and by CCh microinjection into the PHN of adrenergic- and V1-vasopressin receptor-blocked rats suggests that NPY might be released from sympathetic neurons after activation of the sympathetic nervous system by central administration of CCh into the PHN. Carbachol 328-331 neuropeptide Y Rattus norvegicus 201-204 8762162-9 1996 Comparisons of the results of AChE inhibition which would elevate acetylcholine (ACh) levels with those of carbachol administration revealed that AChE inhibition affects both cholinergic and non-cholinergic mechanisms underlying the two behaviors. Carbachol 107-116 acetylcholinesterase (Cartwright blood group) Homo sapiens 146-150 8776663-9 1996 Differential centrifugation studies revealed that Fyn resides predominantly (> 95%) in the crude plasma membrane fraction and undergoes nicotinic-and carbachol-induced activation. Carbachol 153-162 FYN proto-oncogene, Src family tyrosine kinase Bos taurus 50-53 8856685-3 1996 Here we report that microinjection of carbachol into the taste cortex modulates protein tyrosine phosphorylation similarly to the effect of unfamiliar taste, and that a 180 kDa protein whose tyrosine phosphorylation is enhanced in vivo by carbachol is NR2B. Carbachol 38-47 glutamate ionotropic receptor NMDA type subunit 2B Rattus norvegicus 252-256 8856685-3 1996 Here we report that microinjection of carbachol into the taste cortex modulates protein tyrosine phosphorylation similarly to the effect of unfamiliar taste, and that a 180 kDa protein whose tyrosine phosphorylation is enhanced in vivo by carbachol is NR2B. Carbachol 239-248 glutamate ionotropic receptor NMDA type subunit 2B Rattus norvegicus 252-256 8631834-3 1996 Carbachol induces robust luciferase responses in Jurkat and pheochromocytoma PC12 cells expressing an NFAT-luciferase reporter construct and a Gq-coupled m3 muscarinic receptor. Carbachol 0-9 nuclear factor of activated T-cells 5 Rattus norvegicus 102-106 8631834-5 1996 In PC12 cells expressing a Gi-coupled m2 muscarinic receptor, carbachol induces NFAT-mediated luciferase activity that is strictly dependent upon co-expression of a chimeric G alpha q/alpha i subunit, which confers Gq-effector coupling on Gi-linked receptors. Carbachol 62-71 nuclear factor of activated T-cells 5 Rattus norvegicus 80-84 8626481-3 1996 In this system, the muscarinic agonist carbachol stimulated steady-state PLC activity up to 90-fold in the presence of GTP. Carbachol 39-48 heparan sulfate proteoglycan 2 Homo sapiens 73-76 8735615-3 1996 In addition, in the presence of carbachol, nociceptin increased the intracellular concentration of Ca2+ (EC50 60 nM). Carbachol 32-41 prepronociceptin Homo sapiens 43-53 8735615-3 1996 In addition, in the presence of carbachol, nociceptin increased the intracellular concentration of Ca2+ (EC50 60 nM). Carbachol 32-41 carbonic anhydrase 2 Homo sapiens 99-102 8638850-10 1996 Microinjection of carbachol into the mPRF before halothane administration caused a significant reduction in number of halothane-induced EEG spindles. Carbachol 18-27 Spi-C transcription factor (Spi-1/PU.1 related) Mus musculus 37-41 8732292-5 1996 The Ca2+ response evoked by carbachol (10 microM) was completely blocked by the nAChR antagonist, pancuronium (3 microM), but was not affected by the muscarinic antagonist, atropine (3 microM), or under conditions when Ca2+ entry was blocked by La3+ (50 microM) or diltiazem (10 microM). Carbachol 28-37 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 80-85 8736117-1 1996 We determined the effect of intracerebroventricular (icv) administration of losartan, an angiotensin II (ANG II) subtype 1 receptor (AT1) antagonist, on icv carbachol-induced natriuresis, kaliuresis and antidiuresis in water-loaded male Holtzman rats (250-300 g) with a cannula implanted into the lateral ventricle (LV). Carbachol 157-166 angiotensin II receptor, type 1a Rattus norvegicus 89-136 8609894-2 1996 After transfection with IRK1 and m1 muscarinic receptor genes, tsA cells expressed a cesium-sensitive inwardly rectifying potassium conductance that was reduced on application of the muscarinic receptor agonist carbachol. Carbachol 211-220 potassium inwardly rectifying channel subfamily J member 2 Homo sapiens 24-28 8609894-6 1996 Preincubation with staurosporine or the specific PKC inhibitor calphostin C, before application of carbachol, fully prevented the inhibition of IRK1 by m1 muscarinic receptor stimulation. Carbachol 99-108 potassium inwardly rectifying channel subfamily J member 2 Homo sapiens 144-148 8626438-9 1996 In Xenopus laetis oocytes co-expression of GIRK1 with either the chick M2 or M4 mAChR gave carbamylcholine (10 microm)-stimulated K+ currents of 308 +/-26 nA and 298 +/-29 nA, respectively, which were both Ba2+- and pertussis toxin-sensitive. Carbachol 91-106 potassium inwardly rectifying channel subfamily J member 3 S homeolog Xenopus laevis 43-48 8630331-3 1996 The increase of cytosolic free Ca2+ promoted by gastrin, or carbachol, was abolished by the intracellular Ca2+ chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N",N"-tetraacetic acid (BAPTA, 10 microM). Carbachol 60-69 gastrin Rattus norvegicus 48-55 8882619-9 1996 Endothelin-1 was a potent spasmogen in isolated tracheal airway smooth muscle preparations from control mice (ED70 = concentration producing 70% of contraction induced by 10 microM carbachol = 6.3 nM (95% confidence limits, 4.0-10; n = 6 mice)). Carbachol 181-190 endothelin 1 Mus musculus 0-12 8851175-3 1996 Interestingly, when EoL-1 cells were treated with interferon-gamma, mRNAs for muscarinic M3 and M5 receptors could be detected in these cells, along with an increase in [Ca2+]i and chemotaxis induced by carbachol that could be blocked with 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP) and pirenzepine. Carbachol 203-212 interferon gamma Homo sapiens 50-66 8778283-7 1996 Coexpression of the m1 muscarinic receptor with the dopamine-D2 receptor permitted dopamine to stimulate AC-II in the presence of carbachol. Carbachol 130-139 dopamine receptor D2 Homo sapiens 52-72 8621433-5 1996 Carbachol and bombesin also stimulated JNK activity, while vasoactive intestinal peptide did not. Carbachol 0-9 mitogen-activated protein kinase 8 Rattus norvegicus 39-42 8769893-2 1996 Carbachol treatment increased the levels of intracellular Ca2+ and inositol 1,4,5-trisphosphate (IP3) and enhanced transcription of the TH gene. Carbachol 0-9 tyrosine hydroxylase Rattus norvegicus 136-138 8769893-3 1996 The muscarinic receptor antagonist atropine completely abolished the carbachol effect on TH gene expression. Carbachol 69-78 tyrosine hydroxylase Rattus norvegicus 89-91 8769893-5 1996 Transient transfection analysis of the 5" upstream region of TH gene revealed that the AP1 cis-acting element at -205 to -199 bp was responsible for carbachol stimulation. Carbachol 149-158 tyrosine hydroxylase Rattus norvegicus 61-63 8769893-5 1996 Transient transfection analysis of the 5" upstream region of TH gene revealed that the AP1 cis-acting element at -205 to -199 bp was responsible for carbachol stimulation. Carbachol 149-158 Jun proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 87-90 8769893-7 1996 Thus, Ca(2+)-independent PKC may play a role in carbachol-induced TH gene expression. Carbachol 48-57 tyrosine hydroxylase Rattus norvegicus 66-68 8596722-4 1996 In contrast, two specific peptide inhibitors of Ca2+/calmodulin-dependent protein kinase II (CaM-K II), each partially blocked the effect of carbachol, but not the effect of t-ACPD on IAHP. Carbachol 141-150 calcium/calmodulin dependent protein kinase II gamma Homo sapiens 48-91 8596722-4 1996 In contrast, two specific peptide inhibitors of Ca2+/calmodulin-dependent protein kinase II (CaM-K II), each partially blocked the effect of carbachol, but not the effect of t-ACPD on IAHP. Carbachol 141-150 calcium/calmodulin dependent protein kinase II gamma Homo sapiens 93-102 8779914-9 1996 Serum-, carbachol-, and thapsigargin-stimulated [Ca2+]i elevations were reduced by 90, 60, and > 65%, respectively, in cells treated with IFN-gamma +/- TNF-alpha and 30, 45, and 45%, respectively, in cells treated with TNF-alpha. Carbachol 8-17 interferon gamma Homo sapiens 141-150 8812728-0 1996 Effects of carbamylcholine and pyridostigmine on mitochondrial-bound hexokinase in skeletal muscle and heart. Carbachol 11-26 hexokinase 1 Homo sapiens 69-79 8812728-1 1996 We show here that carbamylcholine (acetylcholine agonist) or pyridostigmine (acetylcholinesterase inhibitor), drugs which are widely used in medical treatments, exerted a rapid reduction in mitochondrial-bound hexokinase. Carbachol 18-33 hexokinase 1 Homo sapiens 210-220 8779914-9 1996 Serum-, carbachol-, and thapsigargin-stimulated [Ca2+]i elevations were reduced by 90, 60, and > 65%, respectively, in cells treated with IFN-gamma +/- TNF-alpha and 30, 45, and 45%, respectively, in cells treated with TNF-alpha. Carbachol 8-17 tumor necrosis factor Homo sapiens 155-164 8779914-9 1996 Serum-, carbachol-, and thapsigargin-stimulated [Ca2+]i elevations were reduced by 90, 60, and > 65%, respectively, in cells treated with IFN-gamma +/- TNF-alpha and 30, 45, and 45%, respectively, in cells treated with TNF-alpha. Carbachol 8-17 tumor necrosis factor Homo sapiens 222-231 8660281-14 1996 Although down regulation of total PKC by up to 90% did not significantly affect the secretory response to carbachol, RO 31-8220, a relatively specific inhibitor of PKC, abolished carbachol-induced secretion in normal as well as in PMA-down-regulated cells. Carbachol 179-188 Prkca Cavia porcellus 164-167 8660281-15 1996 This indicates that a PKC isoform resistant to down regulation by PMA is involved in carbachol- but not in cAMP-mediated secretion. Carbachol 85-94 Prkca Cavia porcellus 22-25