PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 3259134-5 1988 The positions of the cysteine residues in each domain also are conserved, indicating that PS beta G and CEA are two members of the same gene family. Cysteine 21-29 protein S (beta) pseudogene Homo sapiens 90-97 3259134-5 1988 The positions of the cysteine residues in each domain also are conserved, indicating that PS beta G and CEA are two members of the same gene family. Cysteine 21-29 pregnancy specific beta-1-glycoprotein 2 Homo sapiens 104-107 2826990-7 1987 Displacement of 3H-NKA with different tachykinin-related peptides gave the following rank order of potencies: NKA greater than NKA (4-10) greater than kassinin greater than eledoisin greater than NKB much greater than substance P greater than physalaemin, which suggests that the binding site labeled by 3H-NKA is different from substance P (NK-1)-and NKB (NK-3)-binding sites. Tritium 16-18 Natural killer alloreactivity QTL 1 Rattus norvegicus 110-113 2826990-7 1987 Displacement of 3H-NKA with different tachykinin-related peptides gave the following rank order of potencies: NKA greater than NKA (4-10) greater than kassinin greater than eledoisin greater than NKB much greater than substance P greater than physalaemin, which suggests that the binding site labeled by 3H-NKA is different from substance P (NK-1)-and NKB (NK-3)-binding sites. Tritium 16-18 Natural killer alloreactivity QTL 1 Rattus norvegicus 110-113 2826990-7 1987 Displacement of 3H-NKA with different tachykinin-related peptides gave the following rank order of potencies: NKA greater than NKA (4-10) greater than kassinin greater than eledoisin greater than NKB much greater than substance P greater than physalaemin, which suggests that the binding site labeled by 3H-NKA is different from substance P (NK-1)-and NKB (NK-3)-binding sites. Tritium 304-306 Natural killer alloreactivity QTL 1 Rattus norvegicus 19-22 3756906-7 1986 528 IgG2a, but not 225 IgG1, was cytotoxic to A431 cells in the presence of activated peritoneal macrophages, as demonstrated by release of incorporated [3H]thymidine. Tritium 154-156 immunoglobulin heavy variable V1-9 Mus musculus 4-9 3778462-2 1986 In the present work 125I or 3H-labeled ceruloplasmin was chased through the endothelium and the supernate was subjected to affinity chromatography using RCA120 column. Tritium 28-30 ceruloplasmin Homo sapiens 39-52 3019413-7 1986 Among the individual apolipoproteins tested, apolipoprotein A-I and the C apolipoproteins reduced [3H]cholesteryl linoleyl ether uptake, while apolipoprotein E was not effective. Tritium 99-101 apolipoprotein A1 Rattus norvegicus 45-63 3781132-4 1986 At steady state, 93 +/- 4% of the delivered [3H]T-2 was extracted and metabolized by the liver, while 4.6 +/- 0.3% remained unmetabolized in the effluent perfusate. Tritium 45-47 brachyury 2 Rattus norvegicus 48-51 3144065-7 1987 Both IGF1 and EGF increased the [3H]thymidine incorporation into the DNA of bovine granulosa cells in vitro, IGF1 being a more potent mitogen. Tritium 33-35 insulin like growth factor 1 Bos taurus 5-9 3144065-7 1987 Both IGF1 and EGF increased the [3H]thymidine incorporation into the DNA of bovine granulosa cells in vitro, IGF1 being a more potent mitogen. Tritium 33-35 epidermal growth factor Bos taurus 14-17 2442157-6 1987 The IGF-II-stimulated [3H]thymidine incorporation was also dependent on extracellular calcium and was inhibited by cobalt and tetramethlin. Tritium 23-25 insulin-like growth factor 2 Mus musculus 4-10 2442157-8 1987 Pretreatment of primed competent cells with pertussis toxin completely abolished subsequent action of IGF-II on both calcium influx and [3H]thymidine incorporation. Tritium 137-139 insulin-like growth factor 2 Mus musculus 102-108 8577222-3 1995 Binding analysis indicated SCP-2 bound [3H]cholesterol and dehydroergosterol with a Kd of 0.3 and 1.7 microM, respectively, and suggested the presence of a single binding site. Tritium 40-42 sterol carrier protein 2 Homo sapiens 27-32 3307454-8 1987 Incubation of rat anterior pituitary cells with [3H]LHRH yielded metabolites 1-4 but not metabolites 5 or 9 as in homogenates. Tritium 49-51 gonadotropin releasing hormone 1 Rattus norvegicus 52-56 3307454-9 1987 Incubation of [3H]LHRH with porcine follicular granulosa cells resulted in the generation of metabolites 2-7 and 9, similar to the profile in homogenates. Tritium 15-17 gonadotropin releasing hormone 1 Rattus norvegicus 18-22 3773496-6 1986 PLA2 activities in the plasma were measured by using the substrate with a 3H-labeled fatty acid in position 2. Tritium 74-76 phospholipase A2 group IB Rattus norvegicus 0-4 7646443-13 1995 The sensitivity of [3H]IP3 binding in permeabilized hepatocytes to varied ratios of GSSG and GSH suggests that the IP3 receptor responds to an oxidized redox environment such as that found in the lumen of the endoplasmic reticulum. Tritium 20-22 inositol 1,4,5-trisphosphate receptor type 3 Homo sapiens 115-127 2464378-3 1987 We found that SOM and VIP always inhibited cell proliferation for incubation times of 2 to 72 h. In contrast when cells were exposed to SP for 24 h or less, there was inhibition of [3H]thymidine uptake by both Peyer"s patches and splenic lymphocytes. Tritium 182-184 tachykinin 1 Mus musculus 136-138 7646458-4 1995 Both receptor splice variants bound [3H]TRH with a Kd of some 80 nM when binding assays were performed in the presence of guanosine 5"-[beta gamma-imido]-triphosphate. Tritium 37-39 thyrotropin releasing hormone Rattus norvegicus 40-43 3621464-2 1987 The formation of gap junctions was studied by measurement of the incorporation of [3H]hypoxanthine in HGPRT deficient V79 mutant cells after co-cultivation with the hepatocytes. Tritium 83-85 hypoxanthine-guanine phosphoribosyltransferase Cricetulus griseus 102-107 7543575-1 1995 Neuropeptide Y (NPY) is a widely distributed peptide with varied activities including inhibition of [3H]NE secretion from chromaffin cells. Tritium 101-103 neuropeptide Y Bos taurus 0-14 7543575-1 1995 Neuropeptide Y (NPY) is a widely distributed peptide with varied activities including inhibition of [3H]NE secretion from chromaffin cells. Tritium 101-103 neuropeptide Y Bos taurus 16-19 7477436-6 1995 At no time during the treatment, was the specific binding of [3H]8-OH-DPAT (agonist radioligand) or [3H]WAY-100 635 (antagonist radioligand) to 5-HT1A receptors modified in the dorsal raphe nucleus or in other brain areas, suggesting that neither the density nor the coupling of these receptors to G-proteins were probably altered in rats injected with fluoxetine or paroxetine for up to 21 days. Tritium 62-64 5-hydroxytryptamine receptor 1A Rattus norvegicus 144-150 3612088-1 1987 The surface protein gp70 of an ecotropic murine retrovirus was followed during entry of [3H]glucosamine-labelled virions into SC-1 mouse fibroblasts. Tritium 89-91 embigin Mus musculus 20-24 3650602-2 1987 Neutrophil elastase and chymotrypsin digested [3H]dexamethasone 21-mesylate labeled receptors at different sites to produce 52 kDa and 42 kDa fragments respectively. Tritium 47-50 elastase, neutrophil expressed Mus musculus 0-19 7477436-6 1995 At no time during the treatment, was the specific binding of [3H]8-OH-DPAT (agonist radioligand) or [3H]WAY-100 635 (antagonist radioligand) to 5-HT1A receptors modified in the dorsal raphe nucleus or in other brain areas, suggesting that neither the density nor the coupling of these receptors to G-proteins were probably altered in rats injected with fluoxetine or paroxetine for up to 21 days. Tritium 101-103 5-hydroxytryptamine receptor 1A Rattus norvegicus 144-150 7540662-2 1995 Pretreatment of membranes with secretory phospholipase A2 (sPLA2) elicited a concentration-dependent decrease in specific [3H]AMPA binding due mainly to a decrease in affinity (KD). Tritium 123-125 phospholipase A2 group IIA Rattus norvegicus 31-57 2825044-2 1987 At the GABA-receptor complex the barbiturates inhibited the binding of [35S]t-butylbicyclophosphorothionate ([35S]TBPT) and enhanced the binding of [3H]diazepam. Tritium 149-151 GABA type A receptor-associated protein Homo sapiens 7-20 3313005-5 1987 The R-ras p23 protein was found to be 3H labeled in the presence of [9,10(n)-3H]palmitic acid and is associated with the P100 membrane fraction of HT1080 cells. Tritium 38-40 RAS related Homo sapiens 4-9 7540662-2 1995 Pretreatment of membranes with secretory phospholipase A2 (sPLA2) elicited a concentration-dependent decrease in specific [3H]AMPA binding due mainly to a decrease in affinity (KD). Tritium 123-125 phospholipase A2 group IIA Rattus norvegicus 59-64 3313005-5 1987 The R-ras p23 protein was found to be 3H labeled in the presence of [9,10(n)-3H]palmitic acid and is associated with the P100 membrane fraction of HT1080 cells. Tritium 38-40 RAS related Homo sapiens 10-13 7556378-9 1995 Only the angiotensin AT1 receptor subtype interacted with G-proteins, as indicated by the 80% inhibition of [3H]angiotensin II binding in the presence of guanosine 5"-O-(3-thiophosphate) or fluoroaluminates. Tritium 109-111 angiotensin II receptor type 1 Homo sapiens 21-24 8529048-4 1995 Dopamine D2-like receptor sites, which probably belong to the dopamine D2 receptor subtype, were characterized using [3H]-spiroperidol as a ligand. Tritium 118-120 dopamine receptor D2 Homo sapiens 62-82 3109877-1 1987 Enkephalin convertase (carboxypeptidase EH; EC 3.4.17.10), a carboxypeptidase B-like enzyme which processes hormone and neuropeptide precursors, has been characterized in the gastrointestinal tract, submandibular gland, and pancreas using a binding assay with [3H]guanidinoethylmercaptosuccinic acid. Tritium 261-263 carboxypeptidase E Homo sapiens 0-21 2956529-4 1987 The permeability-surface area constant for [3H]quinolinic acid at the rat blood-brain barrier was 1.5 +/- 1.3 X 10(-5) sec-1 compared to 2.8 +/- 0.4 X 10(-5) sec-1 for [3H]mannitol. Tritium 44-46 secretory blood group 1 Rattus norvegicus 119-124 2956529-4 1987 The permeability-surface area constant for [3H]quinolinic acid at the rat blood-brain barrier was 1.5 +/- 1.3 X 10(-5) sec-1 compared to 2.8 +/- 0.4 X 10(-5) sec-1 for [3H]mannitol. Tritium 44-46 secretory blood group 1 Rattus norvegicus 158-163 3036952-4 1987 Thus, the biological activities of the growth factors IL-2 or IL-1 could be detected and quantitated at the same level of sensitivity as with the conventional [3H]thymidine incorporation. Tritium 160-162 interleukin 1 alpha Homo sapiens 62-66 7583680-5 1995 [3H]-spiperone autoradiography performed one day after the termination of dopamine infusion into the striatum revealed a dramatic reduction of D2-dopamine receptor binding. Tritium 1-3 dopamine receptor D2 Rattus norvegicus 143-163 3040036-4 1987 This enzyme which splits the substrate [3H]globin and 125I-bovine serum albumin (125I-BSA) has many similarities to the ATP-dependent proteolytic enzyme system from reticulocytes which utilizes ubiquitin: 1) The specific activities in reticulocyte lysates and cardiac muscle extracts are of the same magnitude (0.5-1 arb. Tritium 40-42 ubiquitin Oryctolagus cuniculus 194-203 7759504-4 1995 These events were observed in parallel with GH-stimulated release of [3H]arachidonic acid ([3H]AA) from cellular phospholipids of rat hepatocytes labeled with [3H]AA. Tritium 70-72 gonadotropin releasing hormone receptor Rattus norvegicus 44-46 3105869-1 1987 To investigate the role of hormones in regulating growth of neoplastic mammary cells, we established a heterologous assay for studying interactions of partially purified calf uterine [3H]estradiol-charged estrogen receptor ([3H]ER) with rat tumor nuclei in vitro. Tritium 184-186 estrogen receptor 1 Rattus norvegicus 205-222 7759504-4 1995 These events were observed in parallel with GH-stimulated release of [3H]arachidonic acid ([3H]AA) from cellular phospholipids of rat hepatocytes labeled with [3H]AA. Tritium 92-94 gonadotropin releasing hormone receptor Rattus norvegicus 44-46 7759504-6 1995 Similarly, when the GH-induced liberation of [3H]AA was blocked by the PLA2 inhibitor mepacrine or the Ca2+ channel blocker verapamil, GH-induced accumulation of P4502C12 mRNA was absent. Tritium 46-48 gonadotropin releasing hormone receptor Rattus norvegicus 20-22 3109408-3 1987 Incubation of [3H]-B(c)Ph with highly purified cytochrome P450c in a reconstituted monooxygenase system followed by trapping of the metabolically formed arene oxides as above indicated that the 3,4-oxide was predominantly the (+)-(3S,4R)-enantiomer (90%) and that the 5,6-oxide consisted mainly of the (+)-(5S,6R)-enantiomer (76%). Tritium 15-17 cytochrome P450, family 1, subfamily a, polypeptide 1 Rattus norvegicus 47-63 7759504-6 1995 Similarly, when the GH-induced liberation of [3H]AA was blocked by the PLA2 inhibitor mepacrine or the Ca2+ channel blocker verapamil, GH-induced accumulation of P4502C12 mRNA was absent. Tritium 46-48 gonadotropin releasing hormone receptor Rattus norvegicus 135-137 7783126-1 1995 In order to explore the structural requirements for high 5-HT1A affinity, a series of aryl-substituted N1-phenylpiperazines were synthesized and evaluated for their ability to displace [3H]-8-OH-DPAT from its specific binding sites in rat frontal cortex homogenates. Tritium 186-188 5-hydroxytryptamine receptor 1A Rattus norvegicus 57-63 3033070-5 1987 MSU-induced supernatants with IL 1 activity were neutralized with rabbit antiserum to human IL 1 and also stimulated the growth ([3H]thymidine incorporation) of long-term fibroblast-like cell lines derived from human synovial rheumatoid exudate. Tritium 130-132 interleukin 1 alpha Homo sapiens 30-34 7670733-6 1995 Antagonist potency at prejunctional alpha 2-adrenoceptors in pithed rat heart was correlated with antagonist affinity at alpha 2-adrenoceptor ligand binding sites in membranes of rat kidney and submandibular gland labelled with [3H]-yohimbine. Tritium 229-231 adrenoceptor alpha 2A Rattus norvegicus 36-57 3311026-1 1987 Insulin and insulin-related growth factor 1 (IGF-1) increase by 1.5-1.6-fold the rate of [3H]leucine incorporation into protein in primary monolayer cultures of chick-embryo fibroblasts (CEF); half-maximal hormone concentrations are 10 and 0.25 nM respectively. Tritium 90-92 insulin like growth factor 1 Gallus gallus 12-43 3311026-1 1987 Insulin and insulin-related growth factor 1 (IGF-1) increase by 1.5-1.6-fold the rate of [3H]leucine incorporation into protein in primary monolayer cultures of chick-embryo fibroblasts (CEF); half-maximal hormone concentrations are 10 and 0.25 nM respectively. Tritium 90-92 insulin like growth factor 1 Gallus gallus 45-50 7624833-5 1995 Pretreatment with haloperidol (2 mg/kg) significantly decreases the radioactivity measured in the brain 30-120 min after injection of [3H]4-PPBP. Tritium 135-138 pro-platelet basic protein Mus musculus 140-144 3107404-2 1987 Hamsters, which are hibernators, have been considered to be "primed" for thermogenesis and thus not to show cold-acclimation effects, but here a significant increase in [3H]GDP-binding capacity was observed (from 0.5 nmol in control to 0.9 nmol GDP/mg in cold-acclimated hamsters), and this increase was paralleled by an increase in thermogenin antigen amount, as measured in an enzyme-linked immunosorbent assay. Tritium 170-172 mitochondrial brown fat uncoupling protein 1 Mesocricetus auratus 333-344 7537270-2 1995 To identify the site(s) of inhibition in the LPS recognition pathway, we analyzed the apparent binding affinities and interactions of 3H-labeled enzymatically deacylated LPS (dLPS) and [3H]LPS with CD14, the LPS receptor, on THP-1 cells. Tritium 134-136 CD14 molecule Homo sapiens 198-202 7537270-2 1995 To identify the site(s) of inhibition in the LPS recognition pathway, we analyzed the apparent binding affinities and interactions of 3H-labeled enzymatically deacylated LPS (dLPS) and [3H]LPS with CD14, the LPS receptor, on THP-1 cells. Tritium 186-188 CD14 molecule Homo sapiens 198-202 3556195-10 1987 Both CTLL contain a [3H]DFP-labeled protein that migrates with a molecular mass of 60 kDa under nonreducing conditions and with 30 kDa under reducing conditions and which can be inactivated by the TSP-1-specific inhibitor H-D-Pro-Phe-Arg-chloromethylketone. Tritium 21-23 tumor suppressor region 1 Mus musculus 197-202 7552250-3 1995 Binding of [3H]Sch-23390 and [3H]spiperone to D1 and D2 dopamine receptors, respectively, and [3H]8-hydroxy-2-(di-n-propylamino)-tetralin ([3H]8OH-DPAT) to 5-HT1A receptors were measured in various brain regions using quantitative autoradiography. Tritium 12-14 dopamine receptor D2 Rattus norvegicus 46-74 3595720-10 1987 NPY reduced the nerve stimulation-evoked [3H]NA overflow from the mesenteric veins via a nifedipine resistant mechanism. Tritium 42-44 neuropeptide Y Homo sapiens 0-3 2881925-5 1987 In the wild-type cells, Thy-1 can be labeled with [3H] mannose, [3H]galactose, [3H]fucose, [3H]ethanolamine, and [3H]palmitic acid. Tritium 64-68 Thy-1 cell surface antigen Homo sapiens 24-29 2874836-6 1986 However, the various enkephalins exerted this effect at different concentrations; only the EC50 values (half-maximal effective concentrations) of their insulinotropic effect were in the same range as the IC50 values of inhibition of [3H]met-enkephalin binding. Tritium 234-236 proenkephalin Rattus norvegicus 21-31 2881925-5 1987 In the wild-type cells, Thy-1 can be labeled with [3H] mannose, [3H]galactose, [3H]fucose, [3H]ethanolamine, and [3H]palmitic acid. Tritium 51-53 Thy-1 cell surface antigen Homo sapiens 24-29 7552250-3 1995 Binding of [3H]Sch-23390 and [3H]spiperone to D1 and D2 dopamine receptors, respectively, and [3H]8-hydroxy-2-(di-n-propylamino)-tetralin ([3H]8OH-DPAT) to 5-HT1A receptors were measured in various brain regions using quantitative autoradiography. Tritium 30-32 dopamine receptor D2 Rattus norvegicus 46-74 7796138-1 1995 alpha 2A-Adrenergic receptor (AR) and non-adrenergic imidazoline receptor (I-R) binding sites have been previously characterized in rat cerebral cortex membranes using the N-substituted oxazoline, [3H]rilmenidine ([3H]Ril) [King, P.R. Tritium 198-200 adrenoceptor alpha 2A Rattus norvegicus 0-28 2882398-4 1987 In the presence of either clonidine or [Leu5]enkephalin, which by themselves inhibited the electrically stimulated release of [3H]NA, the effect of PDB was significantly reduced. Tritium 127-129 proenkephalin Rattus norvegicus 45-55 3494448-3 1987 This report also shows that EGF and TPA stimulate [3H] thymidine incorporation. Tritium 51-53 epidermal growth factor Homo sapiens 28-31 3567645-4 1987 In the frontal cortex where a greater decrease in acetylcholinesterase (AChE) is obtained, a similar but attenuated reversal occurred, the K+-evoked release of [3H]ACh reaching 75% of the control value at 128 days after lesion. Tritium 161-163 acetylcholinesterase Rattus norvegicus 50-70 3018478-6 1986 As described for other physiologically relevant receptor systems, the potency for displacement of [3H]-(+/-)-L-364,718 binding by CCK agonists, but not antagonists, was reduced by guanosine 5"-(beta, gamma-imido)triphosphate and NaCl and enhanced by MgCl2. Tritium 99-101 cholecystokinin Bos taurus 130-133 3018738-5 1986 In addition to regulating the antigenic phenotype of these cells, both interferon gamma and GM-CSF had antiproliferative effects on SCLC lines as determined by [3H]thymidine incorporation and clonal growth in agar. Tritium 161-163 colony stimulating factor 2 Homo sapiens 92-98 3766957-6 1986 The optimal irradiation time for covalent [3H]R5020-PR complex formation was determined by irradiation for up to 5 min, and separation of the products by sodium dodecylsulfate (SDS)-polyacrylamide gel electrophoresis. Tritium 43-45 progesterone receptor Oryctolagus cuniculus 52-54 3766957-13 1986 However, maximal labeling with [3H]R5020 was obtainable with minimal photodestruction of PR which suggests that photolabeled receptor can be used as a marker for PR under nondenaturing conditions. Tritium 32-34 progesterone receptor Oryctolagus cuniculus 162-164 3567645-4 1987 In the frontal cortex where a greater decrease in acetylcholinesterase (AChE) is obtained, a similar but attenuated reversal occurred, the K+-evoked release of [3H]ACh reaching 75% of the control value at 128 days after lesion. Tritium 161-163 acetylcholinesterase Rattus norvegicus 72-76 3567645-5 1987 The compensatory changes in [3H]ACh release were paralleled by changes in AChE in both regions. Tritium 29-31 acetylcholinesterase Rattus norvegicus 74-78 7796138-1 1995 alpha 2A-Adrenergic receptor (AR) and non-adrenergic imidazoline receptor (I-R) binding sites have been previously characterized in rat cerebral cortex membranes using the N-substituted oxazoline, [3H]rilmenidine ([3H]Ril) [King, P.R. Tritium 215-217 adrenoceptor alpha 2A Rattus norvegicus 0-28 3770601-2 1986 The radiotracer, (+)-2-[125I]iodo-LSD, allows the use of gamma-counting rather than the liquid scintillation counting currently used for existing 3H radioimmunoassays. Tritium 146-148 deoxyribonuclease 1 like 3 Homo sapiens 34-37 7796138-8 1995 In forebrain, high levels of [3H]Ril-labelled alpha 2A-AR sites were observed in the anterior olfactory nucleus, the piriform, entorhinal and perirhinal cortices, lateral septum, bed nucleus of the stria terminalis, several thalamic nuclei, the amygdala and the arcuate, dorsomedial and posterior hypothalamic nuclei. Tritium 30-32 adrenoceptor alpha 2A Rattus norvegicus 46-57 2881310-1 1987 Exposure of IEC-6 cells for 24 hr to either gastrin (50-500 ng/ml) or EGF (100-500 ng/ml) significantly stimulated (100-165%) the rate of [3H]thymidine incorporation into DNA (referred to as DNA synthesis) when compared with the corresponding basal levels. Tritium 139-141 epidermal growth factor Rattus norvegicus 70-73 7796139-2 1995 It was found that diazepam inhibited the ability of basic fibroblast growth factor (bFGF) to stimulate [3H]thymidine incorporation; the IC50 was approximately 5 microM. Tritium 104-106 fibroblast growth factor 2 Rattus norvegicus 52-82 7796139-2 1995 It was found that diazepam inhibited the ability of basic fibroblast growth factor (bFGF) to stimulate [3H]thymidine incorporation; the IC50 was approximately 5 microM. Tritium 104-106 fibroblast growth factor 2 Rattus norvegicus 84-88 3016247-2 1986 Slide-mounted sections of rat brain were labeled with [3H]-[2-D-penicillamine, 5-D-penicillamine]enkephalin([3H]DPDPE), a highly selective delta opioid agonist. Tritium 55-57 proenkephalin Rattus norvegicus 97-107 3016247-4 1986 DPDPE and met-enkephalin inhibited [3H]DPDPE binding with high affinity (lC50 values of 6.3 and 13.8 nM, respectively). Tritium 36-38 proenkephalin Rattus norvegicus 14-24 7769798-2 1995 The compound potently displaced [3H]AII from AT1 but not from AT2 receptors in rat adrenal cortex (Ki 3 nM), but did not modify the dissociation rate of the radioligand from the receptors. Tritium 33-35 angiotensin II receptor, type 1a Rattus norvegicus 45-48 3011254-7 1986 Binding of [3H]TCDD to the 9S component was competitively inhibited by incubation in the presence of 2,3,7,8-tetrachlorodibenzofuran, dibenz(a,h)anthracene, and nonradioactive TCDD, all known to be potent agonists for Ah-receptor-mediated induction of aryl hydrocarbon hydroxylase. Tritium 12-14 cytochrome P450 family 1 subfamily A member 2 Homo sapiens 252-280 3027172-2 1987 Anti-idiotypic IgG and Fab both inhibited the binding of [3H]LTB4, but not [3H]N-formylmethionyl-leucylphenylalanine (fMLP), to PMN leukocytes with similar concentration-effect relationships, whereas neither nonimmune rabbit IgG nor Fab had any inhibitory activity. Tritium 58-60 FA complementation group B Homo sapiens 23-26 3027172-4 1987 Anti-idiotypic IgG and Fab inhibited PMN leukocyte chemotactic responses to LTB4, but not fMLP, with concentration-effect relationships resembling those characteristic of the inhibition of binding of [3H] LTB4, without altering the LTB4-induced release of beta-glucuronidase. Tritium 201-203 FA complementation group B Homo sapiens 23-26 7700260-8 1995 Both diastereomers displaced [3H]progesterone from baculovirus-expressed hPR. Tritium 30-32 haptoglobin-related protein Homo sapiens 73-76 2879844-2 1987 Guanosine 5"-triphosphate (GTP) was found to inhibit guinea pig liver transglutaminase activity as measured by [3H]putrescine incorporation into casein. Tritium 112-114 protein-glutamine gamma-glutamyltransferase 2 Cavia porcellus 70-86 3459916-7 1986 These results indicate that a proliferation assay based on the incorporation of [3H]dThd into spleen cells in response to Epo can be used as a quantitative means of assessing and comparing the effects of erythroleukemia-inducing retroviruses on the proliferation of their target cells. Tritium 81-83 erythropoietin Mus musculus 122-125 7850789-3 1995 Beta 2-m acted to stimulate [3H]thymidine incorporation in PC-3 cells in a linear, concentration-dependent and saturable manner in serum-free medium. Tritium 29-31 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 0-6 3487640-1 1986 The binding of [3H]cyclosporine A (CsA) to BALB/c mouse spleen cells was examined with a novel centrifugation assay which rapidly removes free [3H]CsA from cell surfaces with a minimal loss of low affinity specifically bound [3H]CsA. Tritium 16-18 heat shock protein 9 Mus musculus 35-38 3034195-4 1987 In mouse brain membrane preparations CDM inhibited the binding of [3H]-clonidine to alpha 2- adrenoceptors and of [3H]-WB4101 to alpha 1-adrenoceptors with IC50 values of 18.2 and 87 microM, respectively. Tritium 67-69 cadmium resistance Mus musculus 37-40 7852361-4 1995 PDMP abolished serum and IGF-1-stimulated cell proliferation, as measured by a reduction in [3H]thymidine incorporation, protein, and DNA levels. Tritium 93-95 insulin-like growth factor 1 Mus musculus 25-30 3034195-4 1987 In mouse brain membrane preparations CDM inhibited the binding of [3H]-clonidine to alpha 2- adrenoceptors and of [3H]-WB4101 to alpha 1-adrenoceptors with IC50 values of 18.2 and 87 microM, respectively. Tritium 115-117 cadmium resistance Mus musculus 37-40 3034195-8 1987 Interaction of CDM and DCDM with [3H]-WB4101 binding, on the other hand, was more complex, and not of the competitive type. Tritium 34-36 cadmium resistance Mus musculus 15-18 3028403-1 1987 Stimulation of [3H]-arachidonic acid labeled human synovial cells with 3.0 X 10(-10)M recombinant interleukin-1 or tumor necrosis factor resulted in the release of incorporated radiolabel (41.1% and 27.7% respectively). Tritium 16-18 interleukin 1 alpha Homo sapiens 98-136 3028403-2 1987 Analysis of [3H]-arachidonic acid labeled phospholipids showed that interleukin-1 and tumor necrosis factor diminished [3H]-arachidonic acid in phosphatidyl-choline phosphatidylserine and phosphatidylinositol. Tritium 13-15 interleukin 1 alpha Homo sapiens 68-107 3028403-2 1987 Analysis of [3H]-arachidonic acid labeled phospholipids showed that interleukin-1 and tumor necrosis factor diminished [3H]-arachidonic acid in phosphatidyl-choline phosphatidylserine and phosphatidylinositol. Tritium 13-16 interleukin 1 alpha Homo sapiens 68-107 2820832-13 1987 Similarly the Bmax and Kd values for the binding of [3H]naltrexone, [3H]D-Ser2-Thr6-leucine enkephalin and [3H]ethylketocyclazocine to brain membranes prepared from rats kept on 4, 20 and 50% protein concentration did not differ. Tritium 69-71 proenkephalin Rattus norvegicus 92-102 3487640-1 1986 The binding of [3H]cyclosporine A (CsA) to BALB/c mouse spleen cells was examined with a novel centrifugation assay which rapidly removes free [3H]CsA from cell surfaces with a minimal loss of low affinity specifically bound [3H]CsA. Tritium 16-18 heat shock protein 9 Mus musculus 147-150 3487640-1 1986 The binding of [3H]cyclosporine A (CsA) to BALB/c mouse spleen cells was examined with a novel centrifugation assay which rapidly removes free [3H]CsA from cell surfaces with a minimal loss of low affinity specifically bound [3H]CsA. Tritium 16-18 heat shock protein 9 Mus musculus 147-150 3487640-1 1986 The binding of [3H]cyclosporine A (CsA) to BALB/c mouse spleen cells was examined with a novel centrifugation assay which rapidly removes free [3H]CsA from cell surfaces with a minimal loss of low affinity specifically bound [3H]CsA. Tritium 144-146 heat shock protein 9 Mus musculus 35-38 3487640-1 1986 The binding of [3H]cyclosporine A (CsA) to BALB/c mouse spleen cells was examined with a novel centrifugation assay which rapidly removes free [3H]CsA from cell surfaces with a minimal loss of low affinity specifically bound [3H]CsA. Tritium 144-146 heat shock protein 9 Mus musculus 147-150 3487640-1 1986 The binding of [3H]cyclosporine A (CsA) to BALB/c mouse spleen cells was examined with a novel centrifugation assay which rapidly removes free [3H]CsA from cell surfaces with a minimal loss of low affinity specifically bound [3H]CsA. Tritium 144-146 heat shock protein 9 Mus musculus 147-150 3487640-1 1986 The binding of [3H]cyclosporine A (CsA) to BALB/c mouse spleen cells was examined with a novel centrifugation assay which rapidly removes free [3H]CsA from cell surfaces with a minimal loss of low affinity specifically bound [3H]CsA. Tritium 144-146 heat shock protein 9 Mus musculus 35-38 3487640-1 1986 The binding of [3H]cyclosporine A (CsA) to BALB/c mouse spleen cells was examined with a novel centrifugation assay which rapidly removes free [3H]CsA from cell surfaces with a minimal loss of low affinity specifically bound [3H]CsA. Tritium 144-146 heat shock protein 9 Mus musculus 147-150 3487640-1 1986 The binding of [3H]cyclosporine A (CsA) to BALB/c mouse spleen cells was examined with a novel centrifugation assay which rapidly removes free [3H]CsA from cell surfaces with a minimal loss of low affinity specifically bound [3H]CsA. Tritium 144-146 heat shock protein 9 Mus musculus 147-150 3487640-4 1986 Kinetic studies conducted with a wider range of [3H]CsA concentrations revealed two distinct binding sites, with KD"s of 290 nM and 9.6 microM, respectively. Tritium 49-51 heat shock protein 9 Mus musculus 52-55 3487640-5 1986 [3H]CsA bound only nonspecifically to phosphatidylcholine:cholesterol liposomes. Tritium 1-3 heat shock protein 9 Mus musculus 4-7 3487640-6 1986 Specific [3H]CsA binding sites were found in murine WEHI-5 B-lymphoma cells, murine N1E-115 neuroblastoma cells and human A204 rhabdosarcoma cells. Tritium 10-12 heat shock protein 9 Mus musculus 13-16 3017727-5 1986 Furthermore, in vitro receptor binding was measured to investigate whether or not ethanol, within a non-lethal concentration range, would change the binding parameters of the delta receptor ligand [3H][D-ala2, D-Leu5]enkephalin. Tritium 198-200 proenkephalin Rattus norvegicus 217-227 3040984-8 1987 Treatment with phospholipase A2 or mellitin, a stimulant of endogenous PLA2, led to a selective loss of [3H] RO5-4864 binding with no change in the binding of [3H]PK 11195. Tritium 105-107 phospholipase A2 group IB Rattus norvegicus 15-31 3790152-6 1986 Chromatographic analysis of this (3H)CsA-macromolecule complex on a Bio-Gel P-60 exclusion column demonstrates that the molecular weight of this protein(s), likely cyclophilin, is around 15,000-20,000 daltons. Tritium 34-36 interferon induced protein with tetratricopeptide repeats 3 Homo sapiens 76-80 7773546-19 1995 Quantitative autoradiographic studies in rat brain indicated a differential distribution of 5-HT3receptor sites by [3H]-RS 25259-197. Tritium 116-118 5-hydroxytryptamine receptor 3A Rattus norvegicus 92-105 3096991-4 1986 The nature of the fatty acyl group incorporated into the sn-2 position of the [3H]alkyl-acyl-GPC formed was analyzed by argentation chromatography. Tritium 79-81 glycophorin C Rattus norvegicus 93-96 3011797-3 1986 Virtually all the tritium was removed from C-2 during the reaction confirming the absence of an NIH shift. Tritium 18-25 complement C2 Rattus norvegicus 43-46 3096991-6 1986 At 3 min of incubation with [3H]PAF and [3H]lyso-PAF, control PMN had small but significant amounts of [3H]alkyl-acyl-GPC containing tetraenoic fatty acids, the concentration of which gradually increased as the incubation time progressed. Tritium 29-31 glycophorin C Rattus norvegicus 118-121 7607049-5 1995 Addition of Fe-TRF to islet cultures significantly increased [3H]thymidine uptake at a concentration of 45 micrograms/ml compared with baseline (23,149 counts/min per microgram DNA +/- 6387 S.E.M., P < 0.01). Tritium 62-64 interleukin 5 Rattus norvegicus 15-18 3096991-9 1986 While the concentration of [3H]alkyl-acyl-GPC with dienoic fatty acids in the 20:4-depleted cells gradually increased with the progress of incubation time, these molecular species of GPC in the control PMN remained more or less constant. Tritium 28-30 glycophorin C Rattus norvegicus 42-45 3769859-2 1986 The rate of synthesis of 3H-labeled proteins and [3H]mPL was constant for 17 h of incubation, confirming the viability of the placental tissue. Tritium 50-52 myeloproliferative leukemia virus oncogene Mus musculus 53-56 3093479-5 1986 Electrophoretic analysis of stressed cells pulsed with different metabolic precursors revealed that p32 and p34 were radiolabeled with [35S]methionine or 3H-amino acids but not by [3H]mannose or [35S]cysteine. Tritium 154-156 complement component 1, q subcomponent binding protein Mus musculus 100-103 3010743-1 1986 Angiotensin-converting enzyme (ACE) activity of the cerebral microcirculation of anesthetized dogs was measured from cerebral venous outflow curves after bolus injection of a synthetic ACE substrate, [3H]benzoyl-phenylalanyl-alanylproline ([3H]BPAP), into a common carotid artery. Tritium 201-203 angiotensin I converting enzyme Canis lupus familiaris 0-29 3010743-1 1986 Angiotensin-converting enzyme (ACE) activity of the cerebral microcirculation of anesthetized dogs was measured from cerebral venous outflow curves after bolus injection of a synthetic ACE substrate, [3H]benzoyl-phenylalanyl-alanylproline ([3H]BPAP), into a common carotid artery. Tritium 201-203 angiotensin I converting enzyme Canis lupus familiaris 31-34 3010743-1 1986 Angiotensin-converting enzyme (ACE) activity of the cerebral microcirculation of anesthetized dogs was measured from cerebral venous outflow curves after bolus injection of a synthetic ACE substrate, [3H]benzoyl-phenylalanyl-alanylproline ([3H]BPAP), into a common carotid artery. Tritium 201-203 angiotensin I converting enzyme Canis lupus familiaris 185-188 3019576-4 1986 In the presence of concentrations of IL-2 that resulted in suboptimal stimulation of HT-2 cells (5-20% of maximum proliferation), LTB4 at 10(-8) or 10(-10) M produced a several-fold increase in [3H]thymidine incorporation, resulting in levels of [3H]thymidine incorporation that were comparable to those produced by HT-2 cells stimulated with optimal concentrations of IL-2. Tritium 195-197 interleukin 2 Mus musculus 37-41 3707902-3 1986 Chinese hamster ovary cells grown in medium containing low-density lipoprotein (LDL) express high acyl coenzyme A:cholesterol acyltransferase (ACAT) activity as measured by an [3H]oleate pulse. Tritium 177-179 sterol O-acyltransferase 1 Cricetulus griseus 143-147 3495258-2 1986 In the 3H-thymidine uptake assay, one of three rat and one of three human colon tumour cell lines, i.e., r237 and LIM1215, were stimulated by mEGF. Tritium 7-9 epidermal growth factor Mus musculus 142-146 3495258-3 1986 In the clonogenic assay, mEGF stimulated the one clonogenic rat tumour cell line r113, which did not respond in the 3H-thymidine uptake assay, and the human tumour cell line LIM1215. Tritium 116-118 epidermal growth factor Mus musculus 25-29 3027723-2 1986 [3H]-dihydromorphine ([3H]-DHM)3 [3H]-D-Ala2-D-Leu5-enkephalin ([3H]-DADLE) and [3H]-dynorphin A (1-8) ([3H]-DYN) as markers of mu (mu), delta (delta) and Kappa (kappa) sites were utilized respectively. Tritium 1-3 proenkephalin Rattus norvegicus 52-62 7529746-14 1995 125I-labeled LPS and 3H-labeled taxol was reported to bind to J774.1 cells predominantly via CD14 and microtubules, respectively. Tritium 21-23 CD14 antigen Mus musculus 93-97 3027723-2 1986 [3H]-dihydromorphine ([3H]-DHM)3 [3H]-D-Ala2-D-Leu5-enkephalin ([3H]-DADLE) and [3H]-dynorphin A (1-8) ([3H]-DYN) as markers of mu (mu), delta (delta) and Kappa (kappa) sites were utilized respectively. Tritium 1-5 proenkephalin Rattus norvegicus 52-62 3027723-2 1986 [3H]-dihydromorphine ([3H]-DHM)3 [3H]-D-Ala2-D-Leu5-enkephalin ([3H]-DADLE) and [3H]-dynorphin A (1-8) ([3H]-DYN) as markers of mu (mu), delta (delta) and Kappa (kappa) sites were utilized respectively. Tritium 23-25 proenkephalin Rattus norvegicus 52-62 3958047-7 1986 Desialation was confirmed by incubation of endothelium with double-labeled CP (3H label on sialic acid and 125I on the protein part). Tritium 79-81 ceruloplasmin Homo sapiens 75-77 7870045-6 1995 Transfection of WSS-1 cells with cDNAs encoding either beta 2 or beta 3 subunits resulted in a concentration-dependent enhancement of [3H]flunitrazepam binding by these nonvolatile anesthetics. Tritium 135-137 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 55-71 2942782-2 1986 5-HT and the preferential 5-HT1A receptor agonist 8-OH-DPAT [8-hydroxy-2-(di-n-propylamino)tetralin] concentration-dependently inhibited the evoked 3H overflow. Tritium 148-150 5-hydroxytryptamine receptor 1A Homo sapiens 26-41 3768708-1 1986 Rat hippocampal minces were loaded with N-methyl-[3H]acetylcholine ([3H]ACh) in the presence of the "poorly penetrating" acetylcholinesterase (EC 3.1.1.7, AChE) inhibitor echothiophate and the effect of the depolarizing agent veratridine determined on the subcellular storage and release of [3H]ACh and [3H]choline. Tritium 50-52 acetylcholinesterase Rattus norvegicus 121-141 3768708-1 1986 Rat hippocampal minces were loaded with N-methyl-[3H]acetylcholine ([3H]ACh) in the presence of the "poorly penetrating" acetylcholinesterase (EC 3.1.1.7, AChE) inhibitor echothiophate and the effect of the depolarizing agent veratridine determined on the subcellular storage and release of [3H]ACh and [3H]choline. Tritium 49-52 acetylcholinesterase Rattus norvegicus 121-141 3768708-12 1986 Veratridine depolarization of minces also increased the activity of membrane-bound AChE, but this enzyme was not inhibited by the low NVP concentration which prevented the veratridine-induced breakdown of S3 [3H]ACh. Tritium 209-211 acetylcholinesterase Rattus norvegicus 83-87 3026352-4 1986 Experiments using double (3H/14C) labelling, to distinguish pools with different rates of turnover, suggest the major pool of arachidonic acid used for synthesis of lipoxygenase metabolites turns over more slowly than arachidonic acid in diacylglycerol, but at about the same rate as arachidonic acid esterified in phosphatidylcholine or phosphatidylinositol. Tritium 26-28 polyunsaturated fatty acid lipoxygenase ALOX15 Oryctolagus cuniculus 165-177 3083784-13 1986 Biochemical studies have indicated that the molecular weight of FT-1 antigen on leukemia cells is about 100,000 by means of biosynthetic labeling with either [3H]-galactose or [35S]-methionine. Tritium 159-161 AKT interacting protein Mus musculus 64-68 7899461-3 1995 EGF, IGF-I, and bFGF caused significant and dose-dependent increases in [3H]phosphatidic acid (PA) accumulation in the presence of propranolol, a phosphatidic acid phosphohydrolase inhibitor. Tritium 73-75 fibroblast growth factor 2 Rattus norvegicus 16-20 2935540-3 1986 A monoclonal antibody that binds to the 140k complex specifically inhibits the direct binding of 3H-labeled 75,000-D fibronectin cell-binding fragment (f75k) to chicken embryo fibroblasts in suspension. Tritium 97-99 fibronectin 1 Gallus gallus 117-128 3528288-7 1986 When 3H-labeled AMA were used, CSF-H-treated neutrophils released greater amounts of radiolabeled substances than mock-treated cells, indicating a stimulatory effect of CSF-H on the killing capacity of neutrophils. Tritium 5-7 colony stimulating factor 2 Homo sapiens 31-34 3528288-7 1986 When 3H-labeled AMA were used, CSF-H-treated neutrophils released greater amounts of radiolabeled substances than mock-treated cells, indicating a stimulatory effect of CSF-H on the killing capacity of neutrophils. Tritium 5-7 colony stimulating factor 2 Homo sapiens 169-172 3528288-8 1986 This was confirmed by the fact that untreated neutrophils that had internalized 3H-AMA killed the parasites at a faster rate when subsequently incubated with CSF-H. Catalase, but not superoxide dismutase, mannitol, benzoate, or histidine, inhibited neutrophil killing of the 3H-AMA whether the granulocytes had been exposed to CSF-H or not. Tritium 80-82 colony stimulating factor 2 Homo sapiens 327-330 8532598-4 1995 NMB stimulated [3H]arachidonic acid release from C6 cells and the increase in [3H]arachidonic acid release was reversed [APTTL]SP. Tritium 16-18 neuromedin B Homo sapiens 0-3 3769200-4 1986 This study indicates that in some patients with heterozygous FH, abnormalities of [3H]cholesterol efflux from fibroblasts can be detected. Tritium 83-85 low density lipoprotein receptor Homo sapiens 61-63 3003349-6 1986 The binding site for [3H] tryptamine in the choroid plexus (termed T-2) was pharmacologically distinct from that in the rest of the brain (termed T-1); several compounds, including kynuramine, were potent inhibitors of [3H]tryptamine binding at the brain site, but not at the choroid plexus site. Tritium 22-24 brachyury 2 Rattus norvegicus 67-70 2874015-4 1986 When added together, IGF-I and TSH were markedly synergistic in stimulating DNA synthesis, producing increases in 3H-thymidine incorporation that were far greater than the sum of the effects of each alone. Tritium 114-116 insulin-like growth factor 1 Rattus norvegicus 21-26 2430885-4 1986 The association constant (Ka) for the 3H-Aox-red binding population was determined to be 1.02 X 10(7) M-1. Tritium 38-40 acyl-CoA oxidase 1 Rattus norvegicus 41-44 3020473-3 1986 Preincubation of the membrane fraction with enkephalin chloromethyl ketones causes a significant inhibition of /3H/-naloxone binding which cannot be reversed by extensive washing. Tritium 112-114 proenkephalin Rattus norvegicus 44-54 3724260-2 1986 The rate of the cellular uptake of free [3H]cholesterol from lipid vesicles was similar in both LDL-receptor positive lung fibroblasts and in LDL-receptor negative fibroblasts. Tritium 40-44 low density lipoprotein receptor Homo sapiens 96-108 3724260-3 1986 When human LDL was used as the carrier of free [3H]cholesterol, however, the LDL-receptor positive lung fibroblasts incorporated significantly more [3H]cholesterol than did the LDL-receptor negative cells. Tritium 48-50 low density lipoprotein receptor Homo sapiens 77-89 3724260-3 1986 When human LDL was used as the carrier of free [3H]cholesterol, however, the LDL-receptor positive lung fibroblasts incorporated significantly more [3H]cholesterol than did the LDL-receptor negative cells. Tritium 149-151 low density lipoprotein receptor Homo sapiens 77-89 3008013-2 1986 The effect of these treatments on [3H]muscimol binding to the GABA receptor was also determined. Tritium 35-37 GABA type A receptor-associated protein Homo sapiens 62-75 7528929-9 1994 The pharmacological profile of [3H]kainate binding to the recombinant GluR6 resembled the high-affinity [3H]kainate binding sites in rat brain, showing high affinity for domoate (Ki = 5.1 +/- 3.0 nM) and kainate (Kd = 12.9 +/- 2.4 nM). Tritium 32-34 glutamate ionotropic receptor kainate type subunit 2 Rattus norvegicus 70-75 2999128-5 1985 The purified immunoglobulin inhibited the binding of [3H]dihydromorphine in a titrable, saturable, and reversible manner, as well as the binding of the delta-ligand [3H][D-Ala2,D-Leu5]enkephalin, the kappa-ligand [3H] ethylketocyclazocine, and 3H-labeled antagonists. Tritium 166-168 proenkephalin Rattus norvegicus 184-194 2999128-5 1985 The purified immunoglobulin inhibited the binding of [3H]dihydromorphine in a titrable, saturable, and reversible manner, as well as the binding of the delta-ligand [3H][D-Ala2,D-Leu5]enkephalin, the kappa-ligand [3H] ethylketocyclazocine, and 3H-labeled antagonists. Tritium 166-168 proenkephalin Rattus norvegicus 184-194 3018718-3 1986 Binding of [3H]PIA characterized a high-affinity low-capacity receptor while the [3H]NECA displayed lower-affinity, higher-capacity binding. Tritium 12-14 RPTOR independent companion of MTOR complex 2 Homo sapiens 15-18 7528929-9 1994 The pharmacological profile of [3H]kainate binding to the recombinant GluR6 resembled the high-affinity [3H]kainate binding sites in rat brain, showing high affinity for domoate (Ki = 5.1 +/- 3.0 nM) and kainate (Kd = 12.9 +/- 2.4 nM). Tritium 32-35 glutamate ionotropic receptor kainate type subunit 2 Rattus norvegicus 70-75 2999545-3 1985 Opiate agonists inhibited K+-induced 3H-GABA efflux in the following decreasing order of potency: bremazocine greater than D-Ala2-Met5-enkephalinamide (ENK) greater than SKF 10047 much greater than morphine, consistent with the participation of kappa, delta, sigma and to a lesser extent mu opiate receptors respectively. Tritium 37-39 proenkephalin Rattus norvegicus 152-155 2872895-5 1986 Accordingly, the kinetics of 2 microM [3H] SKF 93479 binding to HGT-1 cells revealed a half-time for association of 10 min and a dissociation half-time of 120 min. Tritium 39-41 solute carrier family 25 member 16 Homo sapiens 64-69 7888492-6 1994 OTr assays were accomplished by isolating uterine cell membranes and performing saturation analysis with cold OTAs and tritium-labeled oxytocin. Tritium 119-126 oxytocin receptor Rattus norvegicus 0-3 3009164-12 1986 In cells prelabeled with [3H] arachidonic acid, GnRH significantly increased levels of DAG in incubations lasting 2-5 min. Tritium 25-28 gonadotropin releasing hormone 1 Rattus norvegicus 48-52 3841402-9 1985 NPY depressed the nerve stimulation-evoked 3H-NA release from human submandibular arteries via a prejunctional mechanism which was resistant to nifedipine. Tritium 43-45 neuropeptide Y Homo sapiens 0-3 7872059-4 1994 The prostaglandin F receptor (FP) selective agonist, fluprostenol, was the most potent agonist tested, significantly inhibiting incorporation of [3H]proline into both collagen and noncollagen protein at 10(-11) M, with more than 90% inhibition of collagen synthesis at 10(-8) M. The PGE2 analog, sulprostone, and PGD2 showed activity similar to that of PGE2. Tritium 146-148 prostaglandin F receptor Rattus norvegicus 4-28 2415394-8 1985 Using [3H]thymidine autoradiography and indirect immunofluorescence of the same cells we show a remarkable correlation between cyclin antigen distribution and topographical patterns of DNA synthesis. Tritium 7-9 proliferating cell nuclear antigen Homo sapiens 127-133 2997214-7 1985 Thus, the high affinity binding of (-)-[3H]HBI to membranes prepared from wild-type and cyc- S49 lymphoma cells was stereoselectively inhibited by the active isomers of isoproterenol and propranolol, and was inhibited by GTP. Tritium 40-42 peptidylprolyl isomerase A, pseudogene 1 Mus musculus 88-91 3013077-3 1986 In the present experiments, opiate receptor binding was measured in spinal cord after traumatic injury in rats using the selective radioligands [3H] [D-Ala2, D-Leu5]enkephalin (delta-receptor agonist); [3H] [D-Ala2,MePhe4,Gly-(ol)5]enkephalin (mu-receptor agonist); and [3H]ethylketocyclazocine (kappa-receptor agonist). Tritium 145-147 proenkephalin Rattus norvegicus 165-175 7999113-1 1994 Eotaxin was recently identified as the major eosinophil chemoattractant in bronchoalveolar lavage fluid obtained 3h after allergen challenge of sensitised guinea-pigs. Tritium 113-115 eotaxin Cavia porcellus 0-7 3461852-1 1986 Serotonin and histamine H1, H2 receptor agonists or antagonists inhibited [3H]histamine uptake by HL-60 cells, according to the following order of potency: impromidine greater than 4-MH greater than histamine greater than AET greater than PEA and: cimetidine, histamine greater than diphenhydramine, serotonin. Tritium 75-77 histamine receptor H1 Homo sapiens 14-39 3512592-9 1986 The antibody substantially inhibited the incorporation of [3H]thymidine by these cells in response to hPL, but was less effective in blocking hPL-stimulated [3H]AIB uptake. Tritium 59-61 galectin 1 Homo sapiens 102-105 3715065-1 1986 A pharmacokinetic study with [3H]des-enkephalin-gamma-endorphin (3H-DE gamma E) was performed in rats after the intravenous, subcutaneous and intramuscular route of administration. Tritium 29-32 proenkephalin Rattus norvegicus 37-47 2934978-1 1985 To clarify the relationship between hexosaminidase A (HEX A) activity and GM2-ganglioside hydrolysis in atypical clinical situations of HEX A deficiency, we have developed a simple method to assess GM2-ganglioside metabolism in cultured fibroblasts utilizing GM2 labeled with tritium in the sphingosine portion of the molecule. Tritium 276-283 hexosaminidase subunit alpha Homo sapiens 54-59 3002965-7 1985 The minimal effective dose varied between experiments and ranged from 10(-11) to 10(-8) M. Moreover, proliferation (3H-TdR incorporation) of mouse thymocytes treated with phytohemagglutinin and IL 1 was decreased in a dose-dependent fashion by 1,25(OH)2D3 starting at 10-11) M. This effect might be secondary to a decrease of endogenous IL 2 production. Tritium 116-118 interleukin 2 Mus musculus 337-341 3902860-0 1985 Incorporation of [3H]thymidine by isolated fetal myoblasts and fibroblasts in response to human placental lactogen (HPL): possible mediation of HPL action by release of immunoreactive SM-C. We investigated the actions of human placental lactogen (HPL) and human growth hormone (HGH) on [3H]thymidine incorporation and the release of immunoassayable somatomedin-C (SM-C) by isolated myoblasts, dermal fibroblasts, and costal cartilage explants taken from human fetuses at 11-21 weeks of gestation. Tritium 18-20 galectin 1 Homo sapiens 116-119 3902860-0 1985 Incorporation of [3H]thymidine by isolated fetal myoblasts and fibroblasts in response to human placental lactogen (HPL): possible mediation of HPL action by release of immunoreactive SM-C. We investigated the actions of human placental lactogen (HPL) and human growth hormone (HGH) on [3H]thymidine incorporation and the release of immunoassayable somatomedin-C (SM-C) by isolated myoblasts, dermal fibroblasts, and costal cartilage explants taken from human fetuses at 11-21 weeks of gestation. Tritium 18-20 galectin 1 Homo sapiens 144-147 3902860-0 1985 Incorporation of [3H]thymidine by isolated fetal myoblasts and fibroblasts in response to human placental lactogen (HPL): possible mediation of HPL action by release of immunoreactive SM-C. We investigated the actions of human placental lactogen (HPL) and human growth hormone (HGH) on [3H]thymidine incorporation and the release of immunoassayable somatomedin-C (SM-C) by isolated myoblasts, dermal fibroblasts, and costal cartilage explants taken from human fetuses at 11-21 weeks of gestation. Tritium 18-20 galectin 1 Homo sapiens 144-147 3902860-1 1985 The incorporation of [3H]thymidine by myoblasts and fibroblasts was significantly increased after incubation for 20 hr or 44 hr, and cell number after incubation for 7 days, in the presence of 50-250 ng/ml HPL. Tritium 22-24 galectin 1 Homo sapiens 206-209 3930401-2 1985 Macrophages preincubated overnight with doses of rIFN-gamma from 5 to 500 U/ml killed [3H]thymidine-labeled mouse L929 tumor targets, as measured by the release of [3H]thymidine into the supernatant after 48 h. Counts of macrophages initially infected with leishmania promastigotes showed that rIFN-gamma-pretreated macrophages could both inhibit the replication of and kill the resulting intramacrophage amastigotes over a 7-day period. Tritium 87-89 interferon gamma Rattus norvegicus 49-59 3930401-2 1985 Macrophages preincubated overnight with doses of rIFN-gamma from 5 to 500 U/ml killed [3H]thymidine-labeled mouse L929 tumor targets, as measured by the release of [3H]thymidine into the supernatant after 48 h. Counts of macrophages initially infected with leishmania promastigotes showed that rIFN-gamma-pretreated macrophages could both inhibit the replication of and kill the resulting intramacrophage amastigotes over a 7-day period. Tritium 165-167 interferon gamma Rattus norvegicus 49-59 3515818-6 1986 Progesterone in a high concentration, 1 microgram/ml, inhibited while 0.1 and 1 microgram/ml hPL stimulated the [3H]thymidine incorporation. Tritium 113-115 galectin 1 Homo sapiens 93-96 2869404-3 1986 The selectivity and potency of these agents were first determined by evaluating their IC50 values for inhibition of [3H](Met5)enkephalin hydrolysis by increasingly complex preparations comprising semipurified aminopeptidases, pallidal membranes, and pallidal slices. Tritium 117-119 proenkephalin Rattus norvegicus 126-136 3009340-3 1986 In a comparison of potencies against [3H]-[D-Ala2,D-Leu5] enkephalin (3H-DADLE) and [3H]-[D-Ala2,MePhe4,Gly-ol5] enkephalin (3H-DAGO) as delta and mu tracers, respectively, enkephalin dimer showed a very high affinity, especially for the delta opiate receptors. Tritium 38-40 tripartite motif containing 13 Homo sapiens 52-56 2993419-8 1985 Functional studies revealed that phorbol ester-activated (but not resting) CLL B cells responded to rIL 2 with [3H]thymidine incorporation and with enhanced secretion of IgM. Tritium 112-114 interleukin 2 Rattus norvegicus 100-105 2993419-10 1985 In one patient, stimulation of Tac+ B cells with rIL 2 resulted in enhanced [3H]thymidine incorporation but no change in IgM secretion, as compared with Tac- B cells; in the second patient, stimulation of Tac+ B cells with rIL 2 did not result in [3H]thymidine uptake, but did result in significant IgM secretion. Tritium 77-79 interleukin 2 Rattus norvegicus 49-54 7954433-13 1994 Affinity chromatography of [3H]glucosamine-labeled KM12 cell extracts on immobilized galectin-1 followed by immunoprecipitation from the lactose-eluted material demonstrated that lysosome-associated membrane glycoprotein-1, carcinoembryonic antigen, and nonspecific cross-reacting antigen are the major galectin-1-binding proteins in these cells. Tritium 28-30 lysosomal associated membrane protein 1 Homo sapiens 179-222 2993419-10 1985 In one patient, stimulation of Tac+ B cells with rIL 2 resulted in enhanced [3H]thymidine incorporation but no change in IgM secretion, as compared with Tac- B cells; in the second patient, stimulation of Tac+ B cells with rIL 2 did not result in [3H]thymidine uptake, but did result in significant IgM secretion. Tritium 248-250 interleukin 2 Rattus norvegicus 49-54 2995978-2 1985 Low concentrations of [D-Ala2, D-Leu5]enkephalin selectively inhibit the mu1 binding of [3H]dihydromorphine, leaving mu2 sites, while low morphine concentrations eliminate the mu1 binding of [3H][D-Ala2, D-Leu5]enkephalin, leaving delta sites. Tritium 89-92 proenkephalin Rattus norvegicus 38-48 3002855-2 1986 The chemical modification by DNFB strongly inhibits the reductase activity of the complex, this being accompanied by labelling by [3H]DNFB of core protein I, the apoprotein of b cytochromes and the 12 kDa subunit. Tritium 131-133 ubiquinol-cytochrome c reductase core protein 1 Homo sapiens 142-156 3485427-4 1986 Material specifically immunoabsorbed with Sepharose-anti-C1q antibody from a culture medium of cells that was metabolically labelled with [3H] proline or [35S] methionine demonstrated a polypeptide pattern identical with that of serum C1q on SDS/polyacrylamide-gel electrophoresis. Tritium 139-141 complement C1q A chain Homo sapiens 57-60 3510622-6 1986 With the use of a novel in situ assay which measured the release of [3H]water from [5-3H] uridine in intact cells, we were able to show that one of the earliest biochemical events is the inhibition of ribonucleotide reductase and that the inhibition of thymidylate synthase, which is delayed by approximately 30 min, was indirectly mediated possibly through effects on ribonucleotide reductase. Tritium 69-71 thymidylate synthetase Homo sapiens 253-273 7979373-3 1994 However, the development of ion-exchange and reverse-phase HPLC methods for the purification and quantitation of the kappa- and beta-caseins, along with tritium labeling of the carbohydrate of the kappa-casein in human milk to aid in its detection, provided the tools for probing micelle structure by examining the composition of micelles fractionated according to size by differential centrifugation. Tritium 153-160 casein kappa Homo sapiens 197-209 3699194-6 1986 The resulting E1 and E2 were finally isolated by co-crystallization to constant specific activity and 3H/14C ratio of crystal crops. Tritium 102-104 small nucleolar RNA, H/ACA box 73A Homo sapiens 14-23 3931041-5 1985 When lactase synthesis was expressed as the quantity of microvillus membrane lactase synthesized relative to total microvillus membrane protein synthesized, a significantly greater proportion of 3H-leucine incorporation into lactase was demonstrated in the suckling animals. Tritium 195-197 lactase Rattus norvegicus 5-12 3931041-5 1985 When lactase synthesis was expressed as the quantity of microvillus membrane lactase synthesized relative to total microvillus membrane protein synthesized, a significantly greater proportion of 3H-leucine incorporation into lactase was demonstrated in the suckling animals. Tritium 195-197 lactase Rattus norvegicus 77-84 3931041-5 1985 When lactase synthesis was expressed as the quantity of microvillus membrane lactase synthesized relative to total microvillus membrane protein synthesized, a significantly greater proportion of 3H-leucine incorporation into lactase was demonstrated in the suckling animals. Tritium 195-197 lactase Rattus norvegicus 77-84 3841006-7 1985 Immunopurified, [3H]DM-labeled GBP subjected to NEPHGE reached isoelectric equilibrium after 6 h of electrophoresis at 400 V. A single, broad peak of radioactivity was identified at pH approximately equal to 6.3. Tritium 17-19 transmembrane protein 132A Homo sapiens 31-34 3841006-9 1985 The GBP charge heterogeneity was confirmed by NEPHGE 2D analysis of [3H]DM-labeled GBP prepared directly from crude cytosol. Tritium 69-71 transmembrane protein 132A Homo sapiens 4-7 3841006-9 1985 The GBP charge heterogeneity was confirmed by NEPHGE 2D analysis of [3H]DM-labeled GBP prepared directly from crude cytosol. Tritium 69-71 transmembrane protein 132A Homo sapiens 83-86 2418827-2 1986 After incubation with [3H]leucine the cells secreted radioactively labeled alpha 2-macroglobulin consisting of subunits with a molecular weight of 180,000. Tritium 23-25 alpha-2-macroglobulin Homo sapiens 75-96 2828974-2 1986 In a titrable manner, the Fab fragments noncompetitively inhibited the binding of the mu selective peptide, [3H][D-Ala2, MePhe4, 2Gly-ol5] enkephalin (DAGO) and the delta selective peptide, [3H][D-Pen2, D-Pen5] enkephalin (DPDPE), to neural membranes. Tritium 109-111 proenkephalin Rattus norvegicus 139-149 2828974-2 1986 In a titrable manner, the Fab fragments noncompetitively inhibited the binding of the mu selective peptide, [3H][D-Ala2, MePhe4, 2Gly-ol5] enkephalin (DAGO) and the delta selective peptide, [3H][D-Pen2, D-Pen5] enkephalin (DPDPE), to neural membranes. Tritium 109-111 proenkephalin Rattus norvegicus 211-221 2992504-4 1985 LHRH evoked increase in [3H]IP3 and [3H]IP2 accumulation as early as 30 sec, while the increase in [3H]IP became significant at 2 min. Tritium 25-27 gonadotropin releasing hormone 1 Rattus norvegicus 0-4 2828974-2 1986 In a titrable manner, the Fab fragments noncompetitively inhibited the binding of the mu selective peptide, [3H][D-Ala2, MePhe4, 2Gly-ol5] enkephalin (DAGO) and the delta selective peptide, [3H][D-Pen2, D-Pen5] enkephalin (DPDPE), to neural membranes. Tritium 191-193 proenkephalin Rattus norvegicus 139-149 7522953-5 1994 Gn-RHR was characterized by [3H]Gn-RH binding to plasma membrane preparations. Tritium 29-31 gonadotropin releasing hormone receptor Homo sapiens 0-6 2829009-1 1986 The development of rat brain delta-opioid receptors has been studied in the postnatal period using the highly selective delta-site ligand [3H]-[D-Pen2, D-Pen5] enkephalin (DPDPE) in binding studies. Tritium 139-141 proenkephalin Rattus norvegicus 160-170 2992504-4 1985 LHRH evoked increase in [3H]IP3 and [3H]IP2 accumulation as early as 30 sec, while the increase in [3H]IP became significant at 2 min. Tritium 37-39 gonadotropin releasing hormone 1 Rattus norvegicus 0-4 2992504-4 1985 LHRH evoked increase in [3H]IP3 and [3H]IP2 accumulation as early as 30 sec, while the increase in [3H]IP became significant at 2 min. Tritium 37-39 gonadotropin releasing hormone 1 Rattus norvegicus 0-4 2866714-2 1985 Tritium-labeled prostaglandin E1 and F2 alpha binding was saturable and reversible. Tritium 0-7 small nucleolar RNA, H/ACA box 73A Homo sapiens 30-45 7522953-5 1994 Gn-RHR was characterized by [3H]Gn-RH binding to plasma membrane preparations. Tritium 29-31 gonadotropin releasing hormone 1 Homo sapiens 0-5 2992490-5 1985 Both types of AK- mutants showed reduced uptake and phosphorylation of [3H]formycin B in comparison to the parental (AK+) cells. Tritium 72-74 adenosine kinase Cricetulus griseus 14-16 7877623-1 1994 The effects of holoprotein formation in human CG (hCG) on the reactivities of several of the individual amino groups of each subunit were investigated by differential trace labeling with [3H]acetic anhydride. Tritium 188-190 chorionic gonadotropin subunit beta 5 Homo sapiens 50-53 4063532-6 1985 The assay for PAF acetylhydrolase was based on the release of 3H from PAF that was labelled in the acetate moiety of the sn-2 position. Tritium 62-64 PCNA clamp associated factor Homo sapiens 14-17 7855308-3 1994 TEI-3356 selectively displaced the [3H]PGE2 binding to EP3-expressing cell membranes, but showed very low affinity for both EP1 and EP2. Tritium 36-38 prostaglandin E receptor 3 (subtype EP3) Mus musculus 55-58 4091799-6 1985 [3H]Hydralazine, used as an affinity label for SSAO, shows saturable binding to brown-fat membranes. Tritium 1-3 amine oxidase, copper containing 3 Rattus norvegicus 47-51 3998730-1 1985 The changes in activity of phospholipid methyltransferase I and [3H]S-adenosyl-L-homocysteine ([3H]SAH) binding were determined in cortical membrane preparations from newborn rats and rats 1, 2, and 8 months old. Tritium 96-98 acyl-CoA synthetase medium-chain family member 3 Rattus norvegicus 99-102 4091799-10 1985 3H-labelled material solubilized from [3H]hydralazine-treated membrane pellets by Triton X-100 at that detergent/protein ratio which releases SSAO from membranes shows the same gel-filtration characteristics as SSAO and appears by lentil lectin-agarose affinity chromatography to contain similar carbohydrate moieties. Tritium 0-2 amine oxidase, copper containing 3 Rattus norvegicus 142-146 3998730-2 1985 The activity of phospholipid methyltransferase I and the [3H]SAH binding were significantly greater (respectively, +30 and +40%) in newborn rats than in 1-, 2-, and 8-month-old rats. Tritium 58-60 acyl-CoA synthetase medium-chain family member 3 Rattus norvegicus 61-64 7855308-5 1994 On the other hand, sulprostone strongly displaced the [3H]PGE2 binding to EP1 and EP3, but not to EP2. Tritium 55-57 prostaglandin E receptor 3 (subtype EP3) Mus musculus 82-85 4091799-10 1985 3H-labelled material solubilized from [3H]hydralazine-treated membrane pellets by Triton X-100 at that detergent/protein ratio which releases SSAO from membranes shows the same gel-filtration characteristics as SSAO and appears by lentil lectin-agarose affinity chromatography to contain similar carbohydrate moieties. Tritium 0-2 amine oxidase, copper containing 3 Rattus norvegicus 211-215 4091799-10 1985 3H-labelled material solubilized from [3H]hydralazine-treated membrane pellets by Triton X-100 at that detergent/protein ratio which releases SSAO from membranes shows the same gel-filtration characteristics as SSAO and appears by lentil lectin-agarose affinity chromatography to contain similar carbohydrate moieties. Tritium 39-41 amine oxidase, copper containing 3 Rattus norvegicus 142-146 7943276-5 1994 ET-1 was more potent than ET-3 in the activation of p42 MAPK, induction of MAPK kinase (MAPKK) gel retardation and uptake of [3H]thymidine in ETA-transfected cells, whereas sarafotoxin (S6c) showed no stimulatory effect on the kinases and [3H]thymidine uptake. Tritium 126-128 endothelin receptor type A Homo sapiens 142-145 4091799-10 1985 3H-labelled material solubilized from [3H]hydralazine-treated membrane pellets by Triton X-100 at that detergent/protein ratio which releases SSAO from membranes shows the same gel-filtration characteristics as SSAO and appears by lentil lectin-agarose affinity chromatography to contain similar carbohydrate moieties. Tritium 39-41 amine oxidase, copper containing 3 Rattus norvegicus 211-215 2987638-1 1985 Displacement studies of [3H]-[D-Ala2-MePhe4-Gly-ol5]-enkephalin ([3H]-DAGO) and [3H]-[D-Ala2-D-Leu5]-enkephalin ([3H]-DADL) by the corresponding unlabeled ligands show that there are at least three classes of sites which bind these enkephalin analogs with high affinity. Tritium 25-27 proenkephalin Rattus norvegicus 53-63 3987619-3 1985 These compounds have a range of affinities for the estrogen receptor (ER) and are able to compete with [3H]E2 binding in the uterus in vivo. Tritium 104-106 estrogen receptor 1 Rattus norvegicus 51-68 3877752-5 1985 A fragment of one of these clones, pmegf10, containing the EGF coding region, was tritium-labeled by nick-translation and used as a probe for in situ hybridization to EGF mRNA. Tritium 82-89 epidermal growth factor Mus musculus 59-62 4053057-5 1985 Cysteamine inhibited the spontaneous degradation of N-OH-Trp-P-2 and enhanced the covalent binding of [3H]N-OH-Trp-P-2 to DNA. Tritium 103-105 polycystin 2, transient receptor potential cation channel Mus musculus 111-118 3838767-5 1985 [3H]Thymidine incorporation was inhibited by NK-CIA-containing supernatants in HLA-DR+ but not HLA-DR- bone marrow cell populations stimulated to proliferative by colony-stimulating factor (CSF). Tritium 1-3 colony stimulating factor 2 Homo sapiens 163-188 3838767-5 1985 [3H]Thymidine incorporation was inhibited by NK-CIA-containing supernatants in HLA-DR+ but not HLA-DR- bone marrow cell populations stimulated to proliferative by colony-stimulating factor (CSF). Tritium 1-3 colony stimulating factor 2 Homo sapiens 190-193 3988735-1 1985 Bovine milk lipase was noncovalently bound to a heparin-Sepharose support and a [3H]glycerol/[14C]triolein emulsion was circulated through it. Tritium 81-83 carboxyl ester lipase Homo sapiens 7-18 3930222-2 1985 In cells prelabeled with [3H]arachidonic acid, LHRH caused an increase in the level of [3H]arachidonic acid released in the culture medium, to 125-150% of control levels at the end of a 60-min incubation period. Tritium 25-28 gonadotropin releasing hormone 1 Rattus norvegicus 47-51 3930222-2 1985 In cells prelabeled with [3H]arachidonic acid, LHRH caused an increase in the level of [3H]arachidonic acid released in the culture medium, to 125-150% of control levels at the end of a 60-min incubation period. Tritium 26-28 gonadotropin releasing hormone 1 Rattus norvegicus 47-51 7923647-6 1994 [3H]iloprost, a prostacyclin receptor agonist, specifically bound to the receptor transiently expressed in COS-7 cells. Tritium 1-3 prostaglandin I2 receptor Homo sapiens 16-37 3932087-6 1985 Exposure of myeloblasts to CSF induced increased triatiated thymidine (3H-TdR) incorporation within a few hours, while TPA did not induce 3H-TdR incorporation by itself and was inhibitory to CSF-induced 3H-TdR uptake. Tritium 71-73 colony stimulating factor 2 Homo sapiens 27-30 2988685-5 1985 The mu-agonists, [D-Ala2,MePhe4,Gly-ol5]enkephalin and morphine, displaced a small portion of the binding of [3H]-[D-Pen2,D-Pen5]enkephalin with high affinities. Tritium 110-112 proenkephalin Rattus norvegicus 40-50 2988685-5 1985 The mu-agonists, [D-Ala2,MePhe4,Gly-ol5]enkephalin and morphine, displaced a small portion of the binding of [3H]-[D-Pen2,D-Pen5]enkephalin with high affinities. Tritium 110-112 proenkephalin Rattus norvegicus 129-139 4067997-2 1985 Each of these compounds was tested as an inhibitor of the specific binding of [3H]-17 alpha,21-dimethyl-19-nor-4,9-pregnadiene-3,20-dione (R5020) to the progesterone receptor in rabbit uterine cytosol. Tritium 79-81 progesterone receptor Oryctolagus cuniculus 153-174 7959256-3 1994 Gn-RH receptor was characterized by [3H]Gn-RH binding to plasma membrane preparations. Tritium 37-39 gonadotropin releasing hormone receptor Homo sapiens 0-14 2578523-7 1985 Incorporation of 3H-methyl groups into phospholipids and intracellular cAMP levels both reached a maximum 30 sec after challenge with GEF or bradykinin, and then declined to base-line levels within 2 to 3 min. Tritium 17-19 rho/rac guanine nucleotide exchange factor (GEF) 2 Mus musculus 134-137 7845533-3 1994 We have assessed the distribution of transferrin receptors in the normal and AD hippocampal formation using [3H]-transferrin ([3H]-Tf) binding and tritium film autoradiography, in order to assess the role of the transferrin receptor in AD. Tritium 109-111 transferrin receptor Homo sapiens 37-57 4052436-4 1985 The addition of unlabeled lyso-PAF to incubations initiated with [3H]PAF results in an increase in the amount of lyso-[3H]PAF product formed and a decrease in the final product, [3H]alkylacyl-GPC; however, the total amount of [3H]PAF metabolized remains unchanged. Tritium 66-68 glycophorin C Rattus norvegicus 192-195 4052436-4 1985 The addition of unlabeled lyso-PAF to incubations initiated with [3H]PAF results in an increase in the amount of lyso-[3H]PAF product formed and a decrease in the final product, [3H]alkylacyl-GPC; however, the total amount of [3H]PAF metabolized remains unchanged. Tritium 66-69 glycophorin C Rattus norvegicus 192-195 7845533-3 1994 We have assessed the distribution of transferrin receptors in the normal and AD hippocampal formation using [3H]-transferrin ([3H]-Tf) binding and tritium film autoradiography, in order to assess the role of the transferrin receptor in AD. Tritium 127-129 transferrin receptor Homo sapiens 37-57 3881885-7 1985 The rise of endogenous rat IGF as well as the infused human IGF I and II caused a widening of the tibial epiphysis and an increase of the [3H]thymidine incorporation into costal cartilage. Tritium 139-141 insulin-like growth factor 1 Rattus norvegicus 27-30 7845533-4 1994 In normal brain, [3H]-Tf binding was highest in the pyramidal cell layers with CA2 > dentate gyrus granule cell layer > or = CA1 > CA3 > or = CA4 > subiculum > parahippocampal gyrus. Tritium 18-20 carbonic anhydrase 4 Homo sapiens 142-145 7845533-5 1994 In AD, significant reductions in [3H]-Tf binding were found in CA1, CA2 and CA4 pyramidal cell layers. Tritium 34-36 carbonic anhydrase 4 Homo sapiens 76-79 3968538-5 1985 [3H]Mannose-labelled E1, E2, GP59(E1) and GP43(E2) were digested with Pronase and the glycopeptides separated by gel filtration. Tritium 1-3 small nucleolar RNA, H/ACA box 73A Homo sapiens 29-37 3875510-5 1985 EGF stimulated cell proliferation in the explants measured as [3H]thymidine incorporation in DNA. Tritium 63-65 epidermal growth factor Mus musculus 0-3 7841937-3 1994 The starting time of [3H]thymidine incorporation into EC was reduced by a maximum of 4 h by pretreatment with bFGF for 12 h but not for 3 h. DNA synthesis in EC was inhibited by pretreatment with bFGF for 24 h. The rate of [3H]thymidine incorporation into SMC was accelerated both by persistent treatment with bFGF and pretreatment for 3 h in the presence of 3% FBS. Tritium 22-24 fibroblast growth factor 2 Rattus norvegicus 110-114 2997666-0 1985 Regional differences in mu 1-binding of [3H][D-Ala2,D-Leu5]-enkephalin: comparisons of thalamus and cortex in the rat. Tritium 41-43 proenkephalin Rattus norvegicus 60-70 2997666-2 1985 Competition studies indicated that approximately 60% of [3H][D-Ala2,D-Leu5]-enkephalin ([3H]DADLE) binding in the thalamus was inhibited by low concentrations of morphine (2-5 nM). Tritium 57-60 proenkephalin Rattus norvegicus 76-86 2997666-2 1985 Competition studies indicated that approximately 60% of [3H][D-Ala2,D-Leu5]-enkephalin ([3H]DADLE) binding in the thalamus was inhibited by low concentrations of morphine (2-5 nM). Tritium 57-59 proenkephalin Rattus norvegicus 76-86 2995942-1 1985 The interactions among guanine nucleotides, transition metals and enkephalin (ENK) receptors were investigated by assaying the [3H]ENK binding to brain synaptic membranes. Tritium 128-130 proenkephalin Rattus norvegicus 66-76 2995942-1 1985 The interactions among guanine nucleotides, transition metals and enkephalin (ENK) receptors were investigated by assaying the [3H]ENK binding to brain synaptic membranes. Tritium 128-130 proenkephalin Rattus norvegicus 78-81 2995942-2 1985 In the presence of 100 mM NaCl, GTP and Gpp(NH)p reduced the binding of [3H]ENK at 0 degree C and 25 degrees C, reflecting a decrease mainly in the affinity of the binding sites. Tritium 73-75 proenkephalin Rattus norvegicus 76-79 7841937-3 1994 The starting time of [3H]thymidine incorporation into EC was reduced by a maximum of 4 h by pretreatment with bFGF for 12 h but not for 3 h. DNA synthesis in EC was inhibited by pretreatment with bFGF for 24 h. The rate of [3H]thymidine incorporation into SMC was accelerated both by persistent treatment with bFGF and pretreatment for 3 h in the presence of 3% FBS. Tritium 22-24 fibroblast growth factor 2 Rattus norvegicus 196-200 6440143-4 1984 In a competitive radioimmunoassay using [3H]aflatoxin B1, 3 pmol (1 ng) of aflatoxin B1, aflatoxin B2, or aflatoxin M1 caused 50% inhibition with this antibody. Tritium 41-43 membrane spanning 4-domains A1 Homo sapiens 85-101 6147756-4 1984 We report here that the majority of specific 3H-neurotensin binding sites is present in the guinea pig ileum circular smooth muscle layer, which is known to be innervated by a large proportion of the ileal non-adrenergic inhibitory nerves. Tritium 45-47 neurotensin/neuromedin N Cavia porcellus 48-59 2990038-1 1985 Glucose-6-phosphatase activity in the rat brain in vivo was estimated by measuring the differential loss of tritium and carbon-14 from the glucose pool labeled by a mixture of [2-3H]glucose and [U-14C]glucose. Tritium 108-115 glucose-6-phosphatase catalytic subunit 1 Rattus norvegicus 0-21 2990038-1 1985 Glucose-6-phosphatase activity in the rat brain in vivo was estimated by measuring the differential loss of tritium and carbon-14 from the glucose pool labeled by a mixture of [2-3H]glucose and [U-14C]glucose. Tritium 179-181 glucose-6-phosphatase catalytic subunit 1 Rattus norvegicus 0-21 2408950-7 1985 Concentrations of IGF-I attained in culture media, approximately 0.1 ng/ml, were sufficient to stimulate [3H]thymidine incorporation into B-cells. Tritium 106-108 insulin-like growth factor 1 Rattus norvegicus 18-23 7841937-3 1994 The starting time of [3H]thymidine incorporation into EC was reduced by a maximum of 4 h by pretreatment with bFGF for 12 h but not for 3 h. DNA synthesis in EC was inhibited by pretreatment with bFGF for 24 h. The rate of [3H]thymidine incorporation into SMC was accelerated both by persistent treatment with bFGF and pretreatment for 3 h in the presence of 3% FBS. Tritium 22-24 fibroblast growth factor 2 Rattus norvegicus 196-200 7841937-3 1994 The starting time of [3H]thymidine incorporation into EC was reduced by a maximum of 4 h by pretreatment with bFGF for 12 h but not for 3 h. DNA synthesis in EC was inhibited by pretreatment with bFGF for 24 h. The rate of [3H]thymidine incorporation into SMC was accelerated both by persistent treatment with bFGF and pretreatment for 3 h in the presence of 3% FBS. Tritium 224-226 fibroblast growth factor 2 Rattus norvegicus 110-114 2995863-2 1985 Using [3H]DADLE as the competitor, we demonstrated that sulfonic-acid enkephalin binds to rat brain opiate receptors, and using [3H]DSLET and [3H]DAGO as the competitors, we demonstrated that sulfonic-acid enkephalin binds preferentially to delta-opiate receptors. Tritium 7-9 proenkephalin Rattus norvegicus 70-80 6236274-7 1984 The number of TCGF surface receptors on PC60 cells was measured using purified 3H-TCGF. Tritium 79-81 interleukin 2 Rattus norvegicus 14-18 7841937-3 1994 The starting time of [3H]thymidine incorporation into EC was reduced by a maximum of 4 h by pretreatment with bFGF for 12 h but not for 3 h. DNA synthesis in EC was inhibited by pretreatment with bFGF for 24 h. The rate of [3H]thymidine incorporation into SMC was accelerated both by persistent treatment with bFGF and pretreatment for 3 h in the presence of 3% FBS. Tritium 224-226 fibroblast growth factor 2 Rattus norvegicus 196-200 6236274-7 1984 The number of TCGF surface receptors on PC60 cells was measured using purified 3H-TCGF. Tritium 79-81 interleukin 2 Rattus norvegicus 82-86 2995863-2 1985 Using [3H]DADLE as the competitor, we demonstrated that sulfonic-acid enkephalin binds to rat brain opiate receptors, and using [3H]DSLET and [3H]DAGO as the competitors, we demonstrated that sulfonic-acid enkephalin binds preferentially to delta-opiate receptors. Tritium 129-131 proenkephalin Rattus norvegicus 70-80 2995863-2 1985 Using [3H]DADLE as the competitor, we demonstrated that sulfonic-acid enkephalin binds to rat brain opiate receptors, and using [3H]DSLET and [3H]DAGO as the competitors, we demonstrated that sulfonic-acid enkephalin binds preferentially to delta-opiate receptors. Tritium 129-131 proenkephalin Rattus norvegicus 70-80 7841937-3 1994 The starting time of [3H]thymidine incorporation into EC was reduced by a maximum of 4 h by pretreatment with bFGF for 12 h but not for 3 h. DNA synthesis in EC was inhibited by pretreatment with bFGF for 24 h. The rate of [3H]thymidine incorporation into SMC was accelerated both by persistent treatment with bFGF and pretreatment for 3 h in the presence of 3% FBS. Tritium 224-226 fibroblast growth factor 2 Rattus norvegicus 196-200 7841937-4 1994 In serum-free medium, bFGF (30 ng/ml) stimulated [3H]thymidine incorporation into SMC but not into EC after incubation for 36 h. Together, bFGF (10 ng/ml) and insulin (10 micrograms/ml) synergistically stimulated [3H]thymidine incorporation into EC, but insulin alone did not. Tritium 50-52 fibroblast growth factor 2 Rattus norvegicus 22-26 3888674-3 1985 Moreover, radioactivity was mainly recovered from the alimentary canal 3h after 125I-labelled tomato lectin administration with only traces in the circulation or internal organs. Tritium 71-73 LTL Solanum lycopersicum 101-107 6380584-1 1984 The kinetics of the reduction of quinonoid 2-amino-4-hydroxy-6, 7-dimethyldihydropteridine (DMPH2) catalyzed by bovine liver dihydropteridine reductase were examined with NADH, (S)-NADD, (S)-NADT, and [3H]-NADH as substrates. Tritium 201-204 quinoid dihydropteridine reductase Bos taurus 125-151 7841937-4 1994 In serum-free medium, bFGF (30 ng/ml) stimulated [3H]thymidine incorporation into SMC but not into EC after incubation for 36 h. Together, bFGF (10 ng/ml) and insulin (10 micrograms/ml) synergistically stimulated [3H]thymidine incorporation into EC, but insulin alone did not. Tritium 214-216 fibroblast growth factor 2 Rattus norvegicus 22-26 7935327-4 1994 The equilibrium dissociation constants for specific [3H]neurotensin binding to these mutants were not different from that for the wild-type receptor. Tritium 53-55 neurotensin/neuromedin N Cricetulus griseus 56-67 6726661-2 1984 Radioautography of brain sections incubated with 6 nM [3H]LSD in vitro revealed substantial specific binding in cortex (CTX), especially in layers III to IV and anterior cingulate gyrus, and in areas CA1 and dentate gyrus of hippocampus (HIP). Tritium 55-57 carbonic anhydrase 1 Mus musculus 200-203 4004913-4 1985 administration, accumulation of [G-3H]VNP in tumors was biphasic and progressive for at least 72 hr. Tritium 35-37 immunoglobulin heavy variable 1-64 Mus musculus 38-41 7528680-7 1994 The channel-site ligand [3H]MK 801 ((+)-5-methyl-10,11-dihydro-5H- dibenzo[a,d]cyclo-hepten-5,10-imine hydrogen maleate) showed the affinity ranking NR1-NR2A = NR1-NR2B >> NR1 > NR1-NR2C = NR1-NR2D. Tritium 25-27 glutamate ionotropic receptor NMDA type subunit 2B Homo sapiens 164-168 2581780-1 1985 The binding of [3H]cGMP (guanosine 3",5"-monophosphate) to purified bovine adrenal cGMP-stimulated phosphodiesterase was measured by Millipore filtration on cellulose ester filter. Tritium 16-18 phosphodiesterase 2A Bos taurus 83-116 6431266-11 1984 Binding of mIgG was mediated through the Fab as well as the Fc part of the molecules: the 3H-Fc as well as the 3H-Fab fragment of mIgG bound to IgG-latex. Tritium 111-113 FA complementation group B Homo sapiens 114-117 7528680-7 1994 The channel-site ligand [3H]MK 801 ((+)-5-methyl-10,11-dihydro-5H- dibenzo[a,d]cyclo-hepten-5,10-imine hydrogen maleate) showed the affinity ranking NR1-NR2A = NR1-NR2B >> NR1 > NR1-NR2C = NR1-NR2D. Tritium 25-27 glutamate ionotropic receptor NMDA type subunit 2C Homo sapiens 191-195 7952881-8 1994 The stimulatory effect of AII (3 nM) on [3H]-thymidine incorporation in VSMC was antagonized by the non-selective AT1/AT2 receptor antagonist, [Sar1, Ile8]-AII (IC50 = 5.6 nM), by the AT1A/AT1B receptor antagonist, losartan (IC50 = 10.5 nM) and the AT1 receptor antagonist, L-158809 (IC50 = 0.20 nM). Tritium 41-43 angiotensin II receptor, type 1a Rattus norvegicus 114-117 7952881-8 1994 The stimulatory effect of AII (3 nM) on [3H]-thymidine incorporation in VSMC was antagonized by the non-selective AT1/AT2 receptor antagonist, [Sar1, Ile8]-AII (IC50 = 5.6 nM), by the AT1A/AT1B receptor antagonist, losartan (IC50 = 10.5 nM) and the AT1 receptor antagonist, L-158809 (IC50 = 0.20 nM). Tritium 41-43 angiotensin II receptor, type 2 Rattus norvegicus 118-121 6327663-11 1984 Thus, if it is assumed that the mitochondrial enzyme catalyzing this covalent attachment of heme to synthetic apo-N alpha-[3H]acetyl-(1-25) is a cytochrome c synthetase, the results may be interpreted as indicating that the amino acid sequence of residues 1 to 25 of horse cytochrome c would contain the principal recognition site of the enzyme. Tritium 122-125 cytochrome c, somatic Equus caballus 145-157 6327663-11 1984 Thus, if it is assumed that the mitochondrial enzyme catalyzing this covalent attachment of heme to synthetic apo-N alpha-[3H]acetyl-(1-25) is a cytochrome c synthetase, the results may be interpreted as indicating that the amino acid sequence of residues 1 to 25 of horse cytochrome c would contain the principal recognition site of the enzyme. Tritium 122-125 cytochrome c, somatic Equus caballus 273-285 6427215-17 1984 Gradual processing of circulating proapo -A-I to mature apo-A-I was observed in vivo following pulse-labeling of apo-A-I with [3H]leucine. Tritium 127-129 apolipoprotein A1 Rattus norvegicus 56-63 6427215-17 1984 Gradual processing of circulating proapo -A-I to mature apo-A-I was observed in vivo following pulse-labeling of apo-A-I with [3H]leucine. Tritium 127-129 apolipoprotein A1 Rattus norvegicus 113-120 6330621-3 1984 Incubations of horizontal sections through the hippocampus with [3H]spiperone (1 nM) resulted in dense labeling restricted to the pyramid cell layer in CA1, the parasubiculum and layers I and II of the entorhinal area (EA), while the other hippocampal subfields contained moderate to low binding. Tritium 65-67 carbonic anhydrase 1 Rattus norvegicus 152-155 2986648-5 1985 CV-3988 also inhibited the specific binding of [3H]-PAF to human and guinea-pig platelets with IC50 values of 1.6 X 10(-7) and 1.8 X 10(-7) M, respectively. Tritium 48-50 PCNA clamp associated factor Homo sapiens 52-55 3987685-6 1985 Studies of the interaction between [3H]RU486 and the progesterone receptor have been undertaken with the aim of understanding the antagonistic effect of this compound. Tritium 35-38 progesterone receptor Oryctolagus cuniculus 53-74 2986120-1 1985 Specific binding properties of the tritium-labeled delta opiate receptor agonist [3H][2-D-penicillamine, 5-D-penicillamine]enkephalin [( 3H][D-Pen2, D-Pen5]enkephalin) were characterized in the rat brain and in a mouse neuroblastoma-rat glioma hybrid cell line (NG 108-15). Tritium 35-42 proenkephalin Rattus norvegicus 123-133 2986120-1 1985 Specific binding properties of the tritium-labeled delta opiate receptor agonist [3H][2-D-penicillamine, 5-D-penicillamine]enkephalin [( 3H][D-Pen2, D-Pen5]enkephalin) were characterized in the rat brain and in a mouse neuroblastoma-rat glioma hybrid cell line (NG 108-15). Tritium 82-84 proenkephalin Rattus norvegicus 123-133 6199505-6 1984 The effectiveness of the in vivo cross-linking procedure was demonstrated by the complete inhibition of viral RNA synthesis in treated cells and by direct binding of [3H]AMT to RI molecules in vivo. Tritium 167-169 aminomethyltransferase Homo sapiens 170-173 2986120-1 1985 Specific binding properties of the tritium-labeled delta opiate receptor agonist [3H][2-D-penicillamine, 5-D-penicillamine]enkephalin [( 3H][D-Pen2, D-Pen5]enkephalin) were characterized in the rat brain and in a mouse neuroblastoma-rat glioma hybrid cell line (NG 108-15). Tritium 137-139 proenkephalin Rattus norvegicus 123-133 7952881-8 1994 The stimulatory effect of AII (3 nM) on [3H]-thymidine incorporation in VSMC was antagonized by the non-selective AT1/AT2 receptor antagonist, [Sar1, Ile8]-AII (IC50 = 5.6 nM), by the AT1A/AT1B receptor antagonist, losartan (IC50 = 10.5 nM) and the AT1 receptor antagonist, L-158809 (IC50 = 0.20 nM). Tritium 41-43 angiotensin II receptor, type 1a Rattus norvegicus 184-188 2986120-2 1985 Saturation isotherms of [3H][D-Pen2, D-Pen5]enkephalin binding to rat brain and NG 108-15 membranes gave apparent Kd values of 1-6 nM. Tritium 25-27 proenkephalin Rattus norvegicus 44-54 2986120-5 1985 An apparent regional distribution of [3H][D-Pen2, D-Pen5]enkephalin binding was observed in the rat brain. Tritium 38-41 proenkephalin Rattus norvegicus 57-67 7952881-8 1994 The stimulatory effect of AII (3 nM) on [3H]-thymidine incorporation in VSMC was antagonized by the non-selective AT1/AT2 receptor antagonist, [Sar1, Ile8]-AII (IC50 = 5.6 nM), by the AT1A/AT1B receptor antagonist, losartan (IC50 = 10.5 nM) and the AT1 receptor antagonist, L-158809 (IC50 = 0.20 nM). Tritium 41-43 angiotensin II receptor, type 1a Rattus norvegicus 184-187 6609095-2 1984 Epidermal growth factor (EGF, 19 ng/ml) was used as a reference for maximum growth stimulation and produced a 9-fold increase in [3H]thymidine incorporation. Tritium 130-132 epidermal growth factor Homo sapiens 0-23 8034622-7 1994 Second, high specific activity [3H]deschloroetomoxir (a covalent ligand for CPT I) reacted with a single form of CPT I in liver (approximately 88 kDa) and skeletal muscle (approximately 82 kDa), while proteins of both sizes were labeled in the cardiac myocyte. Tritium 32-34 carnitine palmitoyltransferase 1B Rattus norvegicus 76-81 6609095-2 1984 Epidermal growth factor (EGF, 19 ng/ml) was used as a reference for maximum growth stimulation and produced a 9-fold increase in [3H]thymidine incorporation. Tritium 130-132 epidermal growth factor Homo sapiens 25-28 6320974-4 1984 In fact, the Kd values of [3H]Met-enkephalin and [3H]DHM are enhanced when measured in striata of ethanol-dependent rats. Tritium 27-30 proenkephalin Rattus norvegicus 34-44 6320974-4 1984 In fact, the Kd values of [3H]Met-enkephalin and [3H]DHM are enhanced when measured in striata of ethanol-dependent rats. Tritium 27-29 proenkephalin Rattus norvegicus 34-44 4035669-7 1985 An increase was also observed in the migration of epithelial cells along the villus, as revealed by [3H]thymidine incorporation and autoradiography in rats fed 900 ppm Sn2+ for 4 weeks. Tritium 101-103 solute carrier family 38, member 5 Rattus norvegicus 168-171 2984251-8 1985 For growth-promoting effects, as measured by the incorporation of [3H]thymidine into DNA, confluent retinal endothelial cells responded to IGF-I and MSA by up to threefold increase in the rate of DNA synthesis, whereas confluent aortic endothelial cells did not respond at all. Tritium 67-69 insulin like growth factor 1 Bos taurus 139-144 6093476-6 1984 Rats were then orally dosed with [3H] T-2 toxin and urine and feces were collected for 21 hours after which all animals were killed and tissues excised. Tritium 34-36 brachyury 2 Rattus norvegicus 38-41 8034622-7 1994 Second, high specific activity [3H]deschloroetomoxir (a covalent ligand for CPT I) reacted with a single form of CPT I in liver (approximately 88 kDa) and skeletal muscle (approximately 82 kDa), while proteins of both sizes were labeled in the cardiac myocyte. Tritium 32-34 carnitine palmitoyltransferase 1B Rattus norvegicus 113-118 7953692-3 1994 PTH increased, and calcitonin inhibited, in a dose-dependent way, K(+)-elicited [3H]acetylcholine release, with apparent effective doses 50 of about 10(-9) M. The effect of PTH was inhibited by co-incubation with the PTH receptor antagonist NLe [8-18]-PTH (3-34) amide. Tritium 81-83 calcitonin-related polypeptide alpha Rattus norvegicus 19-29 6320925-0 1984 Effect of polyethyleneglycols (PEG 600 and PEG 6000) and bovine serum albumin on myeloperoxidase-mediated release of [3H]-serotonin from rat peritoneal mast cells in vitro. Tritium 118-120 myeloperoxidase Rattus norvegicus 81-96 6320925-1 1984 Rat peritoneal mast cells in vitro released [3H]-serotonin in the presence of halide (I-), myeloperoxidase (MPO), and a H2O2-generating system. Tritium 45-47 myeloperoxidase Rattus norvegicus 91-106 6320181-6 1984 If, for example, the ratio of eIF-2 X GEF to total (phosphorylated and unphosphorylated) eIF-2 X [3H]GDP was 0.25, the exchange was found to be maximally inhibited when the proportion of eIF-2(alpha P) X [3H]GDP in hte mixture reached 25%. Tritium 98-100 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 30-35 6320181-6 1984 If, for example, the ratio of eIF-2 X GEF to total (phosphorylated and unphosphorylated) eIF-2 X [3H]GDP was 0.25, the exchange was found to be maximally inhibited when the proportion of eIF-2(alpha P) X [3H]GDP in hte mixture reached 25%. Tritium 98-100 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 89-94 6320181-6 1984 If, for example, the ratio of eIF-2 X GEF to total (phosphorylated and unphosphorylated) eIF-2 X [3H]GDP was 0.25, the exchange was found to be maximally inhibited when the proportion of eIF-2(alpha P) X [3H]GDP in hte mixture reached 25%. Tritium 98-100 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 89-94 6320181-6 1984 If, for example, the ratio of eIF-2 X GEF to total (phosphorylated and unphosphorylated) eIF-2 X [3H]GDP was 0.25, the exchange was found to be maximally inhibited when the proportion of eIF-2(alpha P) X [3H]GDP in hte mixture reached 25%. Tritium 205-207 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 30-35 6320181-6 1984 If, for example, the ratio of eIF-2 X GEF to total (phosphorylated and unphosphorylated) eIF-2 X [3H]GDP was 0.25, the exchange was found to be maximally inhibited when the proportion of eIF-2(alpha P) X [3H]GDP in hte mixture reached 25%. Tritium 205-207 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 89-94 6320181-6 1984 If, for example, the ratio of eIF-2 X GEF to total (phosphorylated and unphosphorylated) eIF-2 X [3H]GDP was 0.25, the exchange was found to be maximally inhibited when the proportion of eIF-2(alpha P) X [3H]GDP in hte mixture reached 25%. Tritium 205-207 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 89-94 6320181-10 1984 Incubation of eIF-2 X GEF with excess [3H]GDP leads to the formation of eIF-2 X [3H] GDP and free GEF and, if eIF-2(alpha 32P) X GDP is also present, all of the GEF is converted to eIF-2(alpha 32P) X GEF. Tritium 39-41 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 14-19 3970724-2 1985 Cytochrome P-450-dependent monooxygenases were induced in mouse lung by intratracheal instillation of BaP, Aroclor-1254, or coal gas condensate (CGC) 24 hr before instillation of [3H]BaP, [3H]-2-AA, [14C]-1-NP, or [14C]PNDO. Tritium 180-182 cytochrome P450, family 21, subfamily a, polypeptide 1 Mus musculus 0-16 3970724-2 1985 Cytochrome P-450-dependent monooxygenases were induced in mouse lung by intratracheal instillation of BaP, Aroclor-1254, or coal gas condensate (CGC) 24 hr before instillation of [3H]BaP, [3H]-2-AA, [14C]-1-NP, or [14C]PNDO. Tritium 189-191 cytochrome P450, family 21, subfamily a, polypeptide 1 Mus musculus 0-16 3919058-7 1985 Intravenous administration of the 7 alpha-3H- and 4-14C-labeled cholesterol to a patient with cerebrotendinous xanthomatosis (CTX) resulted in formation of cholestanol which had 70-75% lower 3H/14C ratio. Tritium 42-44 cytochrome P450 family 27 subfamily A member 1 Homo sapiens 126-129 3919058-11 1985 After intravenous administration of 7 beta-3H-labeled 7 alpha-hydroxycholesterol in a patient with CTX, significant amounts of 3H were incorporated into plasma and fecal cholestanol. Tritium 43-45 cytochrome P450 family 27 subfamily A member 1 Homo sapiens 99-102 3994977-7 1985 Monoclonal antibody M-TS-9 (IgM subclass) decreased the rate of binding of [3H]FdUMP to thymidylate synthase in the presence of 5,10-methylenetetrahydrofolate while M-TS-4 (IgG1) did not change the rate of ternary complex formation. Tritium 76-78 thymidylate synthetase Homo sapiens 88-108 6320181-10 1984 Incubation of eIF-2 X GEF with excess [3H]GDP leads to the formation of eIF-2 X [3H] GDP and free GEF and, if eIF-2(alpha 32P) X GDP is also present, all of the GEF is converted to eIF-2(alpha 32P) X GEF. Tritium 39-41 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 72-77 6320181-10 1984 Incubation of eIF-2 X GEF with excess [3H]GDP leads to the formation of eIF-2 X [3H] GDP and free GEF and, if eIF-2(alpha 32P) X GDP is also present, all of the GEF is converted to eIF-2(alpha 32P) X GEF. Tritium 39-41 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 72-77 7953692-4 1994 Incubation of SCG for 120 min with PTH or calcitonin resulted in dose-dependent augmentation or inhibition of K(+)-induced increase of high affinity [3H]choline uptake, respectively, with a maximal effect at 10(-8) M concentration (PTH) and 10(-9) M concentration (calcitonin) and declining at higher concentrations. Tritium 150-152 calcitonin-related polypeptide alpha Rattus norvegicus 42-52 6320181-10 1984 Incubation of eIF-2 X GEF with excess [3H]GDP leads to the formation of eIF-2 X [3H] GDP and free GEF and, if eIF-2(alpha 32P) X GDP is also present, all of the GEF is converted to eIF-2(alpha 32P) X GEF. Tritium 39-41 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 181-203 6320181-10 1984 Incubation of eIF-2 X GEF with excess [3H]GDP leads to the formation of eIF-2 X [3H] GDP and free GEF and, if eIF-2(alpha 32P) X GDP is also present, all of the GEF is converted to eIF-2(alpha 32P) X GEF. Tritium 81-83 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 14-19 6320181-10 1984 Incubation of eIF-2 X GEF with excess [3H]GDP leads to the formation of eIF-2 X [3H] GDP and free GEF and, if eIF-2(alpha 32P) X GDP is also present, all of the GEF is converted to eIF-2(alpha 32P) X GEF. Tritium 81-83 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 72-77 4074410-4 1985 In order to enhance the possibility to demonstrate torasemide and PAH interference, small volumes of [3H]-PAH + [14C]-inulin solutions were directly injected into the peritubular capillary system using calibrated micropipettes. Tritium 102-104 phenylalanine hydroxylase Rattus norvegicus 106-109 4074410-5 1985 When the concentration of microinjected PAH was less than or equal to 0.6 mmol/l, fractional recovery of [3H]-PAH from the micropunctured kidney was not significantly decreased by the diuretics in contrast to probenecid. Tritium 106-108 phenylalanine hydroxylase Rattus norvegicus 40-43 6320181-10 1984 Incubation of eIF-2 X GEF with excess [3H]GDP leads to the formation of eIF-2 X [3H] GDP and free GEF and, if eIF-2(alpha 32P) X GDP is also present, all of the GEF is converted to eIF-2(alpha 32P) X GEF. Tritium 81-83 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 72-77 4074410-5 1985 When the concentration of microinjected PAH was less than or equal to 0.6 mmol/l, fractional recovery of [3H]-PAH from the micropunctured kidney was not significantly decreased by the diuretics in contrast to probenecid. Tritium 106-108 phenylalanine hydroxylase Rattus norvegicus 110-113 7519030-7 1994 Depletion of CD1a+ Langerhans-like cells from the dermal cell preparation, by 95, 74 and 90% in three separate experiments using immunomagnetic beads, reduced 3H-TdR incorporation at optimal responder-to-stimulator cell ratios by 90, 64, and 87%, respectively. Tritium 159-161 CD1a molecule Homo sapiens 13-17 4074410-6 1985 When the concentration of microinjected PAH was greater than or equal to 5 mmol/l, fractional recovery of [3H]-PAH from the micropunctured kidney was significantly decreased by torasemide from 252% (control) to 210% (P less than 0.005), by furosemide to 217% (P less than 0.005), and by probenecid to 205% (P less than 0.01). Tritium 107-109 phenylalanine hydroxylase Rattus norvegicus 40-43 4074410-6 1985 When the concentration of microinjected PAH was greater than or equal to 5 mmol/l, fractional recovery of [3H]-PAH from the micropunctured kidney was significantly decreased by torasemide from 252% (control) to 210% (P less than 0.005), by furosemide to 217% (P less than 0.005), and by probenecid to 205% (P less than 0.01). Tritium 107-109 phenylalanine hydroxylase Rattus norvegicus 111-114 4074410-7 1985 Under these conditions, differential transit times between [3H]-PAH and [14C]-inulin in the micropunctured kidney were decreased from -15 to -4 s in the presence of torasemide (P less than 0.05). Tritium 60-62 phenylalanine hydroxylase Rattus norvegicus 64-67 3884352-10 1985 The in vitro actions of EGF or FGF alone or in combination with dexamethasone were tested for ability to stimulate [3H]-thymidine incorporation into pure cultures of human corneal fibroblasts (HCF) in defined culture medium. Tritium 116-118 epidermal growth factor Homo sapiens 24-27 2999203-1 1985 The specificity of binding of [3H]cGMP to purified bovine adrenal cGMP-stimulated phosphodiesterase was investigated by adding increasing concentrations of unlabelled analogs of cAMP and cGMP. Tritium 31-33 phosphodiesterase 2A Bos taurus 66-99 3973514-5 1985 [3H]Leucine incorporation into apoA-I also was positively correlated with the triglyceride secretion rate. Tritium 1-3 apolipoprotein A1 Rattus norvegicus 31-37 6320486-3 1983 The rate and degree of 3H-serotonin release by CLP is similar to that produced by thrombin. Tritium 23-25 calmodulin like 3 Homo sapiens 47-50 6674289-7 1983 Rats were fed 0, 5, 7.5 or 10% bentonite for 2 wk and then dosed with [3H] T-2 toxin. Tritium 71-73 brachyury 2 Rattus norvegicus 75-78 6317438-2 1983 A heterologous complex is formed by reconstitution of forskolin-Sepharose bound catalytic function from rabbit myocardial membranes with the homogenous [3H]methyl-GTP-binding protein from duck erythrocyte membranes. Tritium 153-155 mitochondrial ribosome-associated GTPase 1 Oryctolagus cuniculus 163-182 2995943-3 1985 In contrast, specific binding of the delta ligand [3H]D-Ala2,D-Leu5-enkephalin was virtually totally eliminated as a result of formaldehyde perfusion. Tritium 51-53 proenkephalin Rattus norvegicus 68-78 7520123-2 1994 [3H]NT binding to membranes prepared from the NTR cDNA-transfected cells displayed specificity and saturability, with an apparent Kd of 1.25 nM and a Bmax of 43.4 pmol/mg of protein (approximately 3.5 x 10(6) binding sites/cell). Tritium 1-3 neurotensin receptor 1 Homo sapiens 46-49 6140060-12 1983 For example, the L-(+)-2-amino-4-phosphonobutyrate isomer was 15 times more active than the D-(-)-form in inhibiting the binding of DL-[3H]-APB. Tritium 136-139 arginyl aminopeptidase Rattus norvegicus 140-143 8058062-11 1994 When the AHD-M1 and AHD-2 cDNAs were inserted into the expression vector pSG5 and transfected into cultured COS cells, 3-5-fold and 100-fold increases, respectively, in the conversion of [3H]retinaldehyde to [3H]retinoic acid could be detected by high performance liquid chromatographic assay. Tritium 188-190 aldehyde dehydrogenase family 1, subfamily A1 Mus musculus 20-25 6192909-4 1983 Further analysis of MM1 by antibody binding inhibition assay with 3H-labeled MM1-gamma 2b-1 antibody revealed that 8 of the 10 antibodies as well as monoclonal anti-Ly-6.2 showed significant inhibition. Tritium 66-68 prefoldin 5 Mus musculus 20-23 6192909-4 1983 Further analysis of MM1 by antibody binding inhibition assay with 3H-labeled MM1-gamma 2b-1 antibody revealed that 8 of the 10 antibodies as well as monoclonal anti-Ly-6.2 showed significant inhibition. Tritium 66-68 prefoldin 5 Mus musculus 77-80 6511794-9 1984 Culture medium containing [3H]leucine-labeled VLDL was incubated with plasma deficient in lipoproteins of rho less than 1.006 g/ml. Tritium 27-29 very low density lipoprotein receptor Gallus gallus 46-50 6511794-10 1984 Within 30 min of incubation at 37 degrees C, 3H-labeled apo-A-I and apo-9 kDa exchanged between VLDL and high-density lipoprotein, whereas apo-37 kDa exchanged between VLDL and the rho greater than 1.21 g/ml fraction. Tritium 45-47 very low density lipoprotein receptor Gallus gallus 96-100 6511794-10 1984 Within 30 min of incubation at 37 degrees C, 3H-labeled apo-A-I and apo-9 kDa exchanged between VLDL and high-density lipoprotein, whereas apo-37 kDa exchanged between VLDL and the rho greater than 1.21 g/ml fraction. Tritium 45-47 very low density lipoprotein receptor Gallus gallus 168-172 6092547-2 1984 [3H]Flunitrazepam reproducibly and irreversibly labeled mainly one protein (P51) in cerebellum and at least two proteins (P51 and P55) in hippocampus at both temperatures. Tritium 1-3 tumor protein p63 Homo sapiens 76-79 8058062-11 1994 When the AHD-M1 and AHD-2 cDNAs were inserted into the expression vector pSG5 and transfected into cultured COS cells, 3-5-fold and 100-fold increases, respectively, in the conversion of [3H]retinaldehyde to [3H]retinoic acid could be detected by high performance liquid chromatographic assay. Tritium 209-211 aldehyde dehydrogenase family 1, subfamily A1 Mus musculus 20-25 6092547-2 1984 [3H]Flunitrazepam reproducibly and irreversibly labeled mainly one protein (P51) in cerebellum and at least two proteins (P51 and P55) in hippocampus at both temperatures. Tritium 1-3 H3 histone pseudogene 44 Homo sapiens 130-133 6619916-4 1983 Neurons were allowed to specifically take up the labeled amine and attempts were made to recover the in vivo formed [3H]5-HT X SBP complex. Tritium 117-119 spermine binding protein Rattus norvegicus 127-130 6619916-8 1983 Studies with [3H]5-HT added just prior to homogenization indicated that the [3H]5-HT X SBP complex had formed intraneuronally, prior to homogenization. Tritium 14-16 spermine binding protein Rattus norvegicus 87-90 7953635-3 1994 Receptor autoradiographic studies with [3H]CGS 21680 (30 nM), a selective adenosine A2a receptor agonist, showed specific binding sites in the hippocampus. Tritium 40-42 adenosine A2a receptor Rattus norvegicus 74-96 6619916-8 1983 Studies with [3H]5-HT added just prior to homogenization indicated that the [3H]5-HT X SBP complex had formed intraneuronally, prior to homogenization. Tritium 77-79 spermine binding protein Rattus norvegicus 87-90 6619916-9 1983 The protein X [3H]5-HT complexes from brain and gut migrated on the gels with apparent molecular weights of 45,000 and 56,000, corresponding to those measured by SDS-PAGE for purified SBP; however, the 45-kilodalton (kd) molecule predominated when the SBP complex was formed in vivo, whereas the 56-kd molecule predominated when the SBP X [3H]5-HT complex was formed with extracted SBP. Tritium 15-17 spermine binding protein Rattus norvegicus 184-187 6619916-9 1983 The protein X [3H]5-HT complexes from brain and gut migrated on the gels with apparent molecular weights of 45,000 and 56,000, corresponding to those measured by SDS-PAGE for purified SBP; however, the 45-kilodalton (kd) molecule predominated when the SBP complex was formed in vivo, whereas the 56-kd molecule predominated when the SBP X [3H]5-HT complex was formed with extracted SBP. Tritium 15-17 spermine binding protein Rattus norvegicus 252-255 6619916-9 1983 The protein X [3H]5-HT complexes from brain and gut migrated on the gels with apparent molecular weights of 45,000 and 56,000, corresponding to those measured by SDS-PAGE for purified SBP; however, the 45-kilodalton (kd) molecule predominated when the SBP complex was formed in vivo, whereas the 56-kd molecule predominated when the SBP X [3H]5-HT complex was formed with extracted SBP. Tritium 15-17 spermine binding protein Rattus norvegicus 252-255 6619916-9 1983 The protein X [3H]5-HT complexes from brain and gut migrated on the gels with apparent molecular weights of 45,000 and 56,000, corresponding to those measured by SDS-PAGE for purified SBP; however, the 45-kilodalton (kd) molecule predominated when the SBP complex was formed in vivo, whereas the 56-kd molecule predominated when the SBP X [3H]5-HT complex was formed with extracted SBP. Tritium 15-17 spermine binding protein Rattus norvegicus 252-255 6619916-11 1983 In any case, newly taken up [3H]5-HT preferentially labels 45-kd SBP. Tritium 29-31 spermine binding protein Rattus norvegicus 65-68 6619916-12 1983 Depletion of endogenous 5-HT by placing animals on a tryptophan-deficient diet increased the amount of exogenous [3H]5-HT bound to SBP in vivo. Tritium 114-116 spermine binding protein Rattus norvegicus 131-134 6577469-4 1983 Experiments in which [3H]lysine was added to the perfusates showed that the apolipoprotein B that accumulated in VLDL was newly synthesized by the liver whereas the small amount of apolipoprotein B found in lipoproteins of higher density appeared to be washed out of extravascular spaces during perfusion. Tritium 22-24 apolipoprotein B Oryctolagus cuniculus 76-92 6436372-5 1984 Although the mechanisms of this effect remain to be elucidated, a direct effect of IL 2 on B cells may be involved, because the addition of IL 2 along with SAC induced a limited but significant increase of 3H-TdR incorporation in the highly enriched B cell population, which showed very little response to PHA and Con A even in the presence of IL 2, and, as mentioned above, IL 2 induced IgG production in the B cell preparation without any supplement of T cells provided the late-acting BCDF fraction was present in the culture. Tritium 206-208 interleukin 2 Mus musculus 83-87 6436372-5 1984 Although the mechanisms of this effect remain to be elucidated, a direct effect of IL 2 on B cells may be involved, because the addition of IL 2 along with SAC induced a limited but significant increase of 3H-TdR incorporation in the highly enriched B cell population, which showed very little response to PHA and Con A even in the presence of IL 2, and, as mentioned above, IL 2 induced IgG production in the B cell preparation without any supplement of T cells provided the late-acting BCDF fraction was present in the culture. Tritium 206-208 interleukin 2 Mus musculus 140-144 6436372-5 1984 Although the mechanisms of this effect remain to be elucidated, a direct effect of IL 2 on B cells may be involved, because the addition of IL 2 along with SAC induced a limited but significant increase of 3H-TdR incorporation in the highly enriched B cell population, which showed very little response to PHA and Con A even in the presence of IL 2, and, as mentioned above, IL 2 induced IgG production in the B cell preparation without any supplement of T cells provided the late-acting BCDF fraction was present in the culture. Tritium 206-208 interleukin 2 Mus musculus 140-144 6436372-5 1984 Although the mechanisms of this effect remain to be elucidated, a direct effect of IL 2 on B cells may be involved, because the addition of IL 2 along with SAC induced a limited but significant increase of 3H-TdR incorporation in the highly enriched B cell population, which showed very little response to PHA and Con A even in the presence of IL 2, and, as mentioned above, IL 2 induced IgG production in the B cell preparation without any supplement of T cells provided the late-acting BCDF fraction was present in the culture. Tritium 206-208 interleukin 2 Mus musculus 140-144 6595180-5 1984 Coincubation of [3H]PGE1 or [3H]PGF2 alpha with only corresponding excess unlabeled PGs resulted in complete disappearance of silver grains from all the subcellular organelles. Tritium 29-31 prostaglandin F synthase 2 Bos taurus 32-36 6595180-8 1984 For both [3H]PGE1 and [3H]PGF2 alpha the plasma membrane associated grains progressively decreased, while the intracellular organelle associated grains increased with time at 38 degrees C. There were no grains in nuclei at 5 min but they appeared at 10 min and increased until 60 or 120 min. Tritium 23-25 prostaglandin F synthase 2 Bos taurus 26-30 6595180-10 1984 In summary, the present studies demonstrate for the first time that exogenously added [3H]PGE1 and [3H]PGF2 alpha internalize in bovine luteal cells in a ligand-specific, time- and temperature-dependent manner. Tritium 100-102 prostaglandin F synthase 2 Bos taurus 103-107 6595180-11 1984 The observations that the internalized [3H]PGE1 does not associate with rough endoplasmic reticulum and that [3H]PGF2 alpha does not associate with rough endoplasmic reticulum and Golgi, even though they contain the binding sites, suggest the presence of mechanism(s) to direct the internalized [3H]PGs to only certain intracellular organelles of luteal cells. Tritium 110-112 prostaglandin F synthase 2 Bos taurus 113-117 6595180-11 1984 The observations that the internalized [3H]PGE1 does not associate with rough endoplasmic reticulum and that [3H]PGF2 alpha does not associate with rough endoplasmic reticulum and Golgi, even though they contain the binding sites, suggest the presence of mechanism(s) to direct the internalized [3H]PGs to only certain intracellular organelles of luteal cells. Tritium 110-112 prostaglandin F synthase 2 Bos taurus 113-117 6196873-5 1983 Ml-1 antibody (IgG2a) reacted with all the panel cells tested and immunoprecipitated a molecule of 43,000 daltons from Nonidet P-40 lysates of 3H-glucosamine-labelled cells. Tritium 143-145 immunoglobulin heavy variable V1-9 Mus musculus 15-20 8079656-4 1994 Elevated G6PD levels in response to loading and PGI2 persisted for 18 h, by which time, ALP activity in surface osteoblasts was elevated and [3H]proline incorporation into collagen increased. Tritium 142-144 glucose-6-phosphate dehydrogenase Rattus norvegicus 9-13 6625605-4 1983 Recovery of progesterone receptor binding was improved by prelabeling with [3H]R5020, by adding 1.5 mM ethylene glycol bis(beta-aminoethylether)N,N"-tetraacetic acid (EGTA) to all buffers, and at high tissue concentrations. Tritium 76-78 progesterone receptor Rattus norvegicus 12-33 6150705-1 1984 The binding of [3H]PAF-acether (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) to intact human gel-filtered platelets was measured at 22 degrees C. Specific binding reached saturation within 15 min at high doses of [3H]PAF-acether (0.5-0.9 nM), whereas about 90 min were required when low doses (0.02-0.5 nM) were used. Tritium 16-18 PCNA clamp associated factor Homo sapiens 19-22 6150705-1 1984 The binding of [3H]PAF-acether (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) to intact human gel-filtered platelets was measured at 22 degrees C. Specific binding reached saturation within 15 min at high doses of [3H]PAF-acether (0.5-0.9 nM), whereas about 90 min were required when low doses (0.02-0.5 nM) were used. Tritium 16-18 PCNA clamp associated factor Homo sapiens 220-223 6150705-2 1984 Above 1 nM, [3H]PAF-acether non-specific binding increased progressively, which together with the demonstration of a 3H-labelled metabolite suggested uptake and metabolism of [3H]PAF-acether. Tritium 13-15 PCNA clamp associated factor Homo sapiens 16-19 7528644-5 1994 MBP P89-101 specificity of cells were measured by 3H-TdR incorporation. Tritium 50-52 myelin basic protein Mus musculus 0-3 6150705-2 1984 Above 1 nM, [3H]PAF-acether non-specific binding increased progressively, which together with the demonstration of a 3H-labelled metabolite suggested uptake and metabolism of [3H]PAF-acether. Tritium 13-15 PCNA clamp associated factor Homo sapiens 179-182 6135604-4 1983 The dopamine-beta-hydroxylase inhibitor diethyldithiocarbamate (DDC), administered 2 h before [3H]E2 to reduce levels of only NE and Ep, increased the total and specific uptake of [3H]E2 by 62-140% in nuclear and supernatant fractions of the anterior pituitary and also increased uptake in several brain areas. Tritium 95-97 dopamine beta-hydroxylase Rattus norvegicus 4-29 6135604-4 1983 The dopamine-beta-hydroxylase inhibitor diethyldithiocarbamate (DDC), administered 2 h before [3H]E2 to reduce levels of only NE and Ep, increased the total and specific uptake of [3H]E2 by 62-140% in nuclear and supernatant fractions of the anterior pituitary and also increased uptake in several brain areas. Tritium 181-183 dopamine beta-hydroxylase Rattus norvegicus 4-29 6412229-2 1983 The apo A-I was labeled with covalently linked 125I-labeled tyramine cellobiose, which accumulates in the cells degrading the apoprotein; [3H]cholesterol ethers, which cannot be hydrolyzed or mobilized after uptake, were incorporated into the lipid core of reconstituted HDL to reflect the fate of the cholesterol esters. Tritium 139-141 apolipoprotein A1 Rattus norvegicus 4-11 6412634-2 1983 3H-Labeled amino acid incorporation into tyrosinase present in the microsome, melanosome, and soluble fractions confirmed the precursor-product relationship of the enzyme in the microsome fraction and in the melanosome fraction. Tritium 0-2 tyrosinase Mus musculus 41-51 6092039-3 1984 Tritium was displaced less readily from C-1 than from C-2 or C-4 of the aromatic ring. Tritium 0-7 complement C2 Rattus norvegicus 54-57 6092897-2 1984 Prior administration of either morphine or D-Ala2-D-Leu5-enkephalin to the homogenates inhibits in a concentration-dependent manner the specific binding of [3H]naloxonazine. Tritium 157-159 proenkephalin Rattus norvegicus 57-67 6090098-0 1984 Pituitary binding of 3H-labeled Sertoli cell factor in vitro: a potential radioreceptor assay for inhibin. Tritium 21-23 KIT ligand Rattus norvegicus 32-51 6090098-1 1984 We have previously demonstrated that binding of partially purified, 3H-labeled Sertoli cell factor (SCF) to rat anterior pituitary homogenates was tissue specific, saturable, time and temperature dependent, and competitively inhibited by unlabeled SCF. Tritium 68-70 KIT ligand Rattus norvegicus 79-98 8197200-4 1994 Upon transient expression in HeLa cells, dSERT exhibited saturable, high-affinity, and sodium-dependent [3H]5HT uptake with estimated Km and Vmax values of approximately 500 nM and 5.2 x 10(-18) mol per cell per min, respectively. Tritium 105-107 Serotonin transporter Drosophila melanogaster 41-46 6090098-1 1984 We have previously demonstrated that binding of partially purified, 3H-labeled Sertoli cell factor (SCF) to rat anterior pituitary homogenates was tissue specific, saturable, time and temperature dependent, and competitively inhibited by unlabeled SCF. Tritium 68-70 KIT ligand Rattus norvegicus 100-103 6090098-1 1984 We have previously demonstrated that binding of partially purified, 3H-labeled Sertoli cell factor (SCF) to rat anterior pituitary homogenates was tissue specific, saturable, time and temperature dependent, and competitively inhibited by unlabeled SCF. Tritium 68-70 KIT ligand Rattus norvegicus 248-251 6090098-2 1984 The present study further characterized the binding of [3H]SCF to rat anterior pituitary in vitro and explored its potential use as a radioreceptor assay for SCF and other inhibin preparations. Tritium 56-58 KIT ligand Rattus norvegicus 59-62 6090098-3 1984 [3H]SCF was synthesized by rat Sertoli cells cultured in the presence of [3H]leucine (5 mu Ci/ml) and was then partially purified by Sephadex gel filtration and high pressure liquid chromatography. Tritium 1-3 KIT ligand Rattus norvegicus 4-7 6875853-0 1983 [3H]Nicotine binding in rat brain: alteration after chronic acetylcholinesterase inhibition. Tritium 1-3 acetylcholinesterase Rattus norvegicus 60-80 8032656-4 1994 Endothelin-1 (ET-1), 5-hydroxytryptamine (5-HT) and the protein kinase C (PKC) activator tetradecanoylphorbol acetate (TPA), stimulated a time- and concentration-dependent increase in [3H]-phosphatidylbutanol accumulation. Tritium 185-187 EDN1 Ovis aries 0-12 6353264-0 1983 Correlation between the distribution of 3H-labelled enkephalin in rat brain and the anatomical regions involved in enkephalin-induced seizures. Tritium 40-42 proenkephalin Rattus norvegicus 52-62 6092561-3 1984 A portion of [3H]-beta-endorphin binding is sensitive to low concentrations of morphine or D-Ala2-Leu5-enkephalin (less than 5 nM). Tritium 14-16 proenkephalin Rattus norvegicus 103-113 6092561-6 1984 The portion of [3H]-beta-endorphin binding that is inhibited under these conditions appears to be equally sensitive to both morphine and the enkephalin and may correspond to mu1 sites. Tritium 16-18 proenkephalin Rattus norvegicus 141-151 6353264-2 1983 administered delta agonist [3H](D-ala2,D-leu5)-enkephalin ([3H]DADL) and the anatomical regions involved in enkephalin-induced seizures has been studied in rat by using an autoradiographic method and recording of the electromyogram (EMG) and the electroencephalogram (EEG). Tritium 28-30 proenkephalin Rattus norvegicus 47-57 8032656-4 1994 Endothelin-1 (ET-1), 5-hydroxytryptamine (5-HT) and the protein kinase C (PKC) activator tetradecanoylphorbol acetate (TPA), stimulated a time- and concentration-dependent increase in [3H]-phosphatidylbutanol accumulation. Tritium 185-187 EDN1 Ovis aries 14-18 6353264-2 1983 administered delta agonist [3H](D-ala2,D-leu5)-enkephalin ([3H]DADL) and the anatomical regions involved in enkephalin-induced seizures has been studied in rat by using an autoradiographic method and recording of the electromyogram (EMG) and the electroencephalogram (EEG). Tritium 60-62 proenkephalin Rattus norvegicus 47-57 6332147-7 1984 The [3H]dsDNA/anti-DNA complexes, which were released from the cells as intact antigen/antibody/complement complexes, did not rebind to RBC, but did bind to Raji cells and could be precipitated by monoclonal antibody to C3d. Tritium 5-7 endogenous retrovirus group K member 13 Homo sapiens 220-223 8163582-2 1994 At 30-min of reperfusion after 20 min of forebrain ischemia, [3H]nimodipine binding was significantly increased in striatum, CA3 and CA4, and dentate relative to binding in sham-operated rats, suggesting that VSCCs were responding to ischemic depolarization. Tritium 62-64 carbonic anhydrase 4 Rattus norvegicus 133-136 6611369-3 1984 By using [3H]phorbol dibutyrate ([3H]PDBu), both to stimulate MER-switch and assay binding of PDBu to CLL cells, it was shown that MER-switch was an irreversible, time-dependent event which occurred some time after maximal binding of [3H]PDBu to cells. Tritium 10-12 MER proto-oncogene, tyrosine kinase Homo sapiens 62-65 6611369-3 1984 By using [3H]phorbol dibutyrate ([3H]PDBu), both to stimulate MER-switch and assay binding of PDBu to CLL cells, it was shown that MER-switch was an irreversible, time-dependent event which occurred some time after maximal binding of [3H]PDBu to cells. Tritium 10-12 MER proto-oncogene, tyrosine kinase Homo sapiens 131-134 6136279-1 1983 The subcellular distributions of the precursor form and mature form of gamma-glutamyltranspeptidase of rat kidney were studied by labeling the enzyme with [3H]fucose in vivo. Tritium 156-158 gamma-glutamyltransferase 1 Rattus norvegicus 71-99 8163517-14 1994 [3H]MC photolabeling of the human Ah receptor from LS180 cells was detected only in experiments using enriched cytosolic preparations. Tritium 1-3 aryl hydrocarbon receptor Homo sapiens 34-45 6344654-3 1983 [3H]LHRH was microinfused at 1.5 X 10(-5 M (concentration 10(6)-10(7) higher than in plasma) and analysis of urinary metabolites was performed by high-performance liquid chromatography. Tritium 1-3 gonadotropin releasing hormone 1 Rattus norvegicus 4-8 6611369-3 1984 By using [3H]phorbol dibutyrate ([3H]PDBu), both to stimulate MER-switch and assay binding of PDBu to CLL cells, it was shown that MER-switch was an irreversible, time-dependent event which occurred some time after maximal binding of [3H]PDBu to cells. Tritium 34-36 MER proto-oncogene, tyrosine kinase Homo sapiens 62-65 6611369-3 1984 By using [3H]phorbol dibutyrate ([3H]PDBu), both to stimulate MER-switch and assay binding of PDBu to CLL cells, it was shown that MER-switch was an irreversible, time-dependent event which occurred some time after maximal binding of [3H]PDBu to cells. Tritium 34-36 MER proto-oncogene, tyrosine kinase Homo sapiens 131-134 6611369-3 1984 By using [3H]phorbol dibutyrate ([3H]PDBu), both to stimulate MER-switch and assay binding of PDBu to CLL cells, it was shown that MER-switch was an irreversible, time-dependent event which occurred some time after maximal binding of [3H]PDBu to cells. Tritium 34-36 MER proto-oncogene, tyrosine kinase Homo sapiens 62-65 6611369-3 1984 By using [3H]phorbol dibutyrate ([3H]PDBu), both to stimulate MER-switch and assay binding of PDBu to CLL cells, it was shown that MER-switch was an irreversible, time-dependent event which occurred some time after maximal binding of [3H]PDBu to cells. Tritium 34-36 MER proto-oncogene, tyrosine kinase Homo sapiens 131-134 6611369-5 1984 Binding of [3H]PDBu was inhibited by phorbol ester analogs that stimulated MER-switch, but not by inactive analogs. Tritium 12-14 MER proto-oncogene, tyrosine kinase Homo sapiens 75-78 6208480-9 1984 EGF strongly stimulated [3H]thymidine incorporation in confluent cells, increased final saturation density three to fourfold, and increased whole-cell levels of phosphotyrosine about threefold. Tritium 25-27 epidermal growth factor Homo sapiens 0-3 6334278-3 1984 EGF was shown to stimulate [3H]TdR incorporation into DNA into several organs as previously reported. Tritium 28-30 epidermal growth factor Mus musculus 0-3 6467009-1 1984 Previous radioautographic experiments demonstrated that binding sites labeled by [3H]5-HT and [3H]LSD in rat brain were seen in all layers of CA1, CA4 and the dentate gyrus but not in fields CA2 and CA3 of the hippocampus. Tritium 82-84 carbonic anhydrase 1 Rattus norvegicus 142-145 6467009-1 1984 Previous radioautographic experiments demonstrated that binding sites labeled by [3H]5-HT and [3H]LSD in rat brain were seen in all layers of CA1, CA4 and the dentate gyrus but not in fields CA2 and CA3 of the hippocampus. Tritium 95-97 carbonic anhydrase 1 Rattus norvegicus 142-145 6222914-4 1983 The substrate for these studies was bovine rhodopsin labeled by in vitro techniques with [3H]-glucosamine or [3H]-mannose, the former being the derivative most extensively examined. Tritium 90-92 rhodopsin Bos taurus 43-52 6222914-4 1983 The substrate for these studies was bovine rhodopsin labeled by in vitro techniques with [3H]-glucosamine or [3H]-mannose, the former being the derivative most extensively examined. Tritium 110-112 rhodopsin Bos taurus 43-52 6302432-1 1983 Treatment of rat cerebellar membranes with phospholipase A2 (PLA2) or phospholipase C (PLC) increased basal [3H]diazepam binding at 0 degrees C with concomitant disappearance of the stimulatory effect of Cl- ion on the binding. Tritium 109-111 phospholipase A2 group IB Rattus norvegicus 43-59 6302432-1 1983 Treatment of rat cerebellar membranes with phospholipase A2 (PLA2) or phospholipase C (PLC) increased basal [3H]diazepam binding at 0 degrees C with concomitant disappearance of the stimulatory effect of Cl- ion on the binding. Tritium 109-111 phospholipase A2 group IB Rattus norvegicus 61-65 8144547-11 1994 A slight decrease in SMC proliferation as judged by DNA content, [3H]thymidine incorporation, and steady-state levels of histone H3.2 mRNA resulted from TGF-beta 1 treatment. Tritium 66-68 transforming growth factor beta 1 Bos taurus 153-163 6188531-2 1983 In serum free RPMI 1640 medium, FTS stimulated [3H]thymidine incorporation into the DNA of young adult mouse thymocytes in a time and concentration dependent manner. Tritium 48-50 AKT interacting protein Mus musculus 32-35 6090160-1 1984 Barbiturates and the related depressant drugs, etazolate and etomidate, inhibited both the binding of [3H]bicuculline methochloride (BMC) to gamma-aminobutyric acid (GABA) receptor sites and the binding of [3H] beta-carboline-3-carboxylic acid methyl ester (beta CCM) to benzodiazepine receptor sites in mammalian brain. Tritium 103-105 GABA type A receptor-associated protein Homo sapiens 141-180 6540339-3 1984 It was possible to quantitate [3H]estradiol binding to SSB by performing the measurements in the presence of excess diethylstilbestrol, which saturates the estrogen receptor and does not bind to SSB, and by using excess progesterone to determine nonspecific binding. Tritium 31-33 Sjogren syndrome antigen B Gallus gallus 55-58 7515824-4 1994 We examined the effects of interferon-gamma 1) on mucin exocytosis, measured as release of [3H]glucosamine-labeled macromolecules trapped at the stacking/running gel interface of polyacrylamide gels, and 2) on mucin biosynthesis, examined at the RNA level using a cDNA probe directed to the MUC2 mucin gene. Tritium 92-94 LOC100508689 Homo sapiens 50-55 6327910-6 1984 When [3H]D-ala2-D-leu5-enkephalin was used as ligand, microsomes possessed 60% fewer high-affinity sites than did synaptic membranes, and a large number of low-affinity sites. Tritium 6-8 proenkephalin Rattus norvegicus 23-33 6327968-3 1984 Plasma ACE activity was also determined radiometrically with [3H] BPAP as substrate. Tritium 61-64 angiotensin-converting enzyme Oryctolagus cuniculus 7-10 6408762-0 1983 Microautoradiographic study on the tissue localization of liposome-entrapped or unentrapped 3H-labeled beta-galactosidase injected into rats. Tritium 92-94 galactosidase, beta 1 Rattus norvegicus 103-121 7898565-6 1994 Androgen-binding protein in the epididymal lumen may be required to maintain uphill proluminal movement of 3H-androgen into the tubules. Tritium 107-109 sex hormone binding globulin Rattus norvegicus 0-24 6406252-2 1983 CHW-1102, which is deficient in hypoxanthine guanine phosphoribosyl transferase (HGPRT+), incorporated a [3H]purine metabolite(s) from medium in which B82 cells, but not V79, A9 and BHK cells, had been grown for 24 h with [3H]hypoxanthine. Tritium 106-108 hypoxanthine-guanine phosphoribosyltransferase Cricetulus griseus 32-79 6406252-2 1983 CHW-1102, which is deficient in hypoxanthine guanine phosphoribosyl transferase (HGPRT+), incorporated a [3H]purine metabolite(s) from medium in which B82 cells, but not V79, A9 and BHK cells, had been grown for 24 h with [3H]hypoxanthine. Tritium 106-108 hypoxanthine-guanine phosphoribosyltransferase Cricetulus griseus 81-87 6406252-2 1983 CHW-1102, which is deficient in hypoxanthine guanine phosphoribosyl transferase (HGPRT+), incorporated a [3H]purine metabolite(s) from medium in which B82 cells, but not V79, A9 and BHK cells, had been grown for 24 h with [3H]hypoxanthine. Tritium 223-225 hypoxanthine-guanine phosphoribosyltransferase Cricetulus griseus 81-87 6133770-1 1983 The histamine H2-receptor antagonist cimetidine potently inhibited [3H]muscimol and enhanced [3H]flunitrazepam binding in membranes prepared from several brain regions in the rat, including the dorsal raphe nucleus. Tritium 68-70 histamine receptor H 2 Rattus norvegicus 4-25 6206436-1 1984 The relative potency of substance P, substance K and neuromedin K on [3H]substance P binding has been compared in rat brain membrane preparations. Tritium 70-72 tachykinin precursor 3 Homo sapiens 53-65 6202319-11 1984 Two alpha 2M-kallikrein light chain complexes incorporated [3H]diisopropyl fluorophosphate ( [3H]DFP) whereas the Mr 330 000 complex did not react with [3H]DFP. Tritium 60-62 alpha-2-macroglobulin Homo sapiens 4-12 6202319-11 1984 Two alpha 2M-kallikrein light chain complexes incorporated [3H]diisopropyl fluorophosphate ( [3H]DFP) whereas the Mr 330 000 complex did not react with [3H]DFP. Tritium 94-96 alpha-2-macroglobulin Homo sapiens 4-12 6133770-1 1983 The histamine H2-receptor antagonist cimetidine potently inhibited [3H]muscimol and enhanced [3H]flunitrazepam binding in membranes prepared from several brain regions in the rat, including the dorsal raphe nucleus. Tritium 94-96 histamine receptor H 2 Rattus norvegicus 4-25 8025534-1 1994 We have examined the ability of divalent cations to inhibit 3H-GR 65630 binding to 5-HT3 receptor recognition sites in rat brain cortical membranes. Tritium 60-62 5-hydroxytryptamine receptor 3A Rattus norvegicus 83-97 6296341-6 1983 Rats were fed diets containing 0, 5, 12.5 or 20% alfalfa for 2 weeks and then dosed orally with [3H]T-2 toxin. Tritium 97-99 brachyury 2 Rattus norvegicus 100-103 6296341-8 1983 Residual 3H in kidney and muscle was reduced with alfalfa feeding when [3H]T-2 toxin was administered orally. Tritium 9-11 brachyury 2 Rattus norvegicus 75-78 6296341-8 1983 Residual 3H in kidney and muscle was reduced with alfalfa feeding when [3H]T-2 toxin was administered orally. Tritium 72-74 brachyury 2 Rattus norvegicus 75-78 6304550-6 1983 We conclude that the biphasic off-kinetics of [3H]leucine enkephalin is not evidence for a two-site binding model. Tritium 47-49 proenkephalin Rattus norvegicus 58-68 6608576-8 1984 The molecular weight of FT-1 antigen on leukemia cells was estimated to be 130,000 by means of biosynthetic labeling with [3H]galactose and [35S]methionine. Tritium 123-125 AKT interacting protein Mus musculus 24-28 6716181-5 1984 The similar effect of FBP was also observed when the biliary excretion of 3H-labeled folate compounds was investigated in situ. Tritium 74-76 glycine N-methyltransferase Rattus norvegicus 22-25 6330592-3 1984 An age dependent change in the maximum number of 3H-PrCC binding sites in the CA1 and CA4/area dentate subfields of the hippocampus were assessed by using a saturating (6 nM) concentration of 3H-PrCC. Tritium 49-51 carbonic anhydrase 1 Rattus norvegicus 78-81 6330592-4 1984 Although 3H-PrCC specific binding at a saturating concentration (6 nM) was significantly less in both the CA1 and CA4/area dentata subfields of the senescent rat, the magnitude of the decrease was greater in the CA1 region. Tritium 9-11 carbonic anhydrase 1 Rattus norvegicus 106-109 6326476-1 1984 GABA receptor activity, as measured by [3H]muscimol binding, was investigated in visual and auditory cortex of 5 weeks old kittens with different visual experience. Tritium 40-42 GABA type A receptor-associated protein Homo sapiens 0-13 6336756-6 1983 [35S]Methionine and [3H]glucosamine incorporation into Mac-3 by purified macrophages show it is a glycoprotein synthesized by these cells. Tritium 21-23 lysosomal-associated membrane protein 2 Mus musculus 55-60 8208308-2 1994 Displacement of the specific [3H]harman binding in bovine adrenal medulla and rat liver by several beta-carbolines and monoamine oxidase (MAO) inhibitors revealed the pharmacological profile of a single, high-affinity binding site (KD 4.92 +/- 0.43 nmol/l, Bmax 8.47 +/- 0.17 pmol/mg protein; adrenal medulla) which corresponded to the active site of MAO type A (MAO-A). Tritium 30-32 monoamine oxidase A Rattus norvegicus 138-141 6335051-5 1984 Under the enkephalin influence the inclusion of [3H]tyrosine and [14C]lisine in water-soluble and water-insoluble proteins of different cerebral structures lowered. Tritium 49-51 proenkephalin Rattus norvegicus 10-20 8106454-5 1994 In contrast, only AT2 receptor-specific antagonists were potent inhibitors of [3H]ARA release (CGP 42112A IC50 approximately 7 nM, EXP 3880 IC50 approximately 2 nM, and PD 123177 IC50 approximately 10 nM). Tritium 79-81 angiotensin II receptor type 2 Homo sapiens 18-21 6324150-2 1984 In the standard radioreceptor assays, DHET components displaced the CNS-receptor binding of [3H]-enkephalin (ENK) and [3H]thyrotropin releasing hormone (TRH). Tritium 93-95 proenkephalin Rattus norvegicus 97-107 6324150-2 1984 In the standard radioreceptor assays, DHET components displaced the CNS-receptor binding of [3H]-enkephalin (ENK) and [3H]thyrotropin releasing hormone (TRH). Tritium 93-95 proenkephalin Rattus norvegicus 109-112 6320181-5 1984 To understand the nature of the molecular lesion in eIF-2-alpha phosphorylation we used a system of pure components in which the rate of exchange of eIF-2-bound [3H]GDP with unlabeled GDP (via the reaction eIF-2-GDP + GEF in equilibrium eIF-2-GEF + GDP) was measured by using mixtures of eIF-2(alpha P) X [eH]GDP and eIF-2 X [3H]GDP in different proportions at constant concentration of eIF-2 X GEF. Tritium 162-164 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 149-154 6320181-5 1984 To understand the nature of the molecular lesion in eIF-2-alpha phosphorylation we used a system of pure components in which the rate of exchange of eIF-2-bound [3H]GDP with unlabeled GDP (via the reaction eIF-2-GDP + GEF in equilibrium eIF-2-GEF + GDP) was measured by using mixtures of eIF-2(alpha P) X [eH]GDP and eIF-2 X [3H]GDP in different proportions at constant concentration of eIF-2 X GEF. Tritium 162-164 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 149-154 6320181-5 1984 To understand the nature of the molecular lesion in eIF-2-alpha phosphorylation we used a system of pure components in which the rate of exchange of eIF-2-bound [3H]GDP with unlabeled GDP (via the reaction eIF-2-GDP + GEF in equilibrium eIF-2-GEF + GDP) was measured by using mixtures of eIF-2(alpha P) X [eH]GDP and eIF-2 X [3H]GDP in different proportions at constant concentration of eIF-2 X GEF. Tritium 162-164 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 149-154 6291693-3 1982 For the delta-site, [(3)H]-[D-Ala(2),D-Leu(5)]enkephalin was used in the presence of unlabelled [D-Ala(2),MePhe(4),Gly-ol(5)]enkephalin in order to suppress cross-reactivity to the mu-binding site. Tritium 20-25 proenkephalin Rattus norvegicus 46-56 7153783-6 1982 Incorporation of [14C]proline and [3H]leucine into extracellular proteins was greater in DMD fibroblast cultures. Tritium 35-37 dystrophin Homo sapiens 89-92 7119865-4 1982 The data indicate that [3H]STX binding increases in the NGF-treated cells by 15- to 20-fold per cell, 3- to 10-fold per mg of protein, and an estimated 7-fold per unit area of membrane. Tritium 24-26 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 Rattus norvegicus 27-30 6320181-5 1984 To understand the nature of the molecular lesion in eIF-2-alpha phosphorylation we used a system of pure components in which the rate of exchange of eIF-2-bound [3H]GDP with unlabeled GDP (via the reaction eIF-2-GDP + GEF in equilibrium eIF-2-GEF + GDP) was measured by using mixtures of eIF-2(alpha P) X [eH]GDP and eIF-2 X [3H]GDP in different proportions at constant concentration of eIF-2 X GEF. Tritium 162-164 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 149-154 7119865-5 1982 The kinetic properties for [3H]STX binding are unchanged, however, by NGF treatment. Tritium 28-30 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 Rattus norvegicus 31-34 6320181-5 1984 To understand the nature of the molecular lesion in eIF-2-alpha phosphorylation we used a system of pure components in which the rate of exchange of eIF-2-bound [3H]GDP with unlabeled GDP (via the reaction eIF-2-GDP + GEF in equilibrium eIF-2-GEF + GDP) was measured by using mixtures of eIF-2(alpha P) X [eH]GDP and eIF-2 X [3H]GDP in different proportions at constant concentration of eIF-2 X GEF. Tritium 162-164 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 149-154 8206647-3 1994 Epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) added singly caused significant increases relative to control in both the uptake of [3H]thymidine into the cellular DNA of subconfluent monolayers and of [3H]proline into collagenase-sensitive protein. Tritium 157-159 epidermal growth factor like 1 Rattus norvegicus 0-23 6360218-0 1983 Direct tritium-labelling into histidine of luteinizing hormone-releasing hormone agonists and use of the tracers in studies on proteolytic breakdown. Tritium 7-14 gonadotropin releasing hormone 1 Rattus norvegicus 43-80 6360218-2 1983 In the present study, conditions for the direct 3H-labelling at the histidine residue of analogs of LHRH were worked out, circumventing the synthesis of precursor peptides for labelling. Tritium 48-50 gonadotropin releasing hormone 1 Rattus norvegicus 100-104 7094890-2 1982 Tritium-labeled SCF (3H-SCF), synthesized by rat Sertoli cells in vitro in the presence of 3H-leucine and partly purified by Sephadex gel chromatography, was used to the ligand. Tritium 0-7 KIT ligand Rattus norvegicus 16-19 7094890-2 1982 Tritium-labeled SCF (3H-SCF), synthesized by rat Sertoli cells in vitro in the presence of 3H-leucine and partly purified by Sephadex gel chromatography, was used to the ligand. Tritium 0-7 KIT ligand Rattus norvegicus 21-27 7200983-4 1982 The relative rate of RBP synthesis was estimated by measuring the extent of incorporation of [3H]leucine, [3H]lysine, and [3H]phenylalanine after a 12-min pulse label into immunoprecipitable RBP, relative to the incorporation of these amino acids into total liver trichloroacetic acid-precipitable protein. Tritium 94-97 retinol binding protein 4 Rattus norvegicus 21-24 7200983-4 1982 The relative rate of RBP synthesis was estimated by measuring the extent of incorporation of [3H]leucine, [3H]lysine, and [3H]phenylalanine after a 12-min pulse label into immunoprecipitable RBP, relative to the incorporation of these amino acids into total liver trichloroacetic acid-precipitable protein. Tritium 94-96 retinol binding protein 4 Rattus norvegicus 21-24 6125418-11 1982 Newly synthesized ([3H]-labelled) hPL was secreted into the medium within two hours. Tritium 20-22 galectin 1 Homo sapiens 34-37 6643499-3 1983 Maximal rates of VLDL [3H]triacylglycerol secretion were reached by 20-25 min into the chase, preceding the initial appearance of 3H-apolipoproteins in the medium at 30-35 min of chase time. Tritium 23-25 very low density lipoprotein receptor Gallus gallus 17-21 6139416-3 1983 Inhibitors of phospholipase A2 [rho-bromo-phenacylbromide (10 microM), trifluoperazine (3 microM), and quinacrine (3 microM) reduced the potassium-stimulated [3H]catecholamine release from synaptosomes to 78, 39, and 55%, respectively, of depolarized controls. Tritium 159-161 phospholipase A2 group IB Rattus norvegicus 14-30 8206647-3 1994 Epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) added singly caused significant increases relative to control in both the uptake of [3H]thymidine into the cellular DNA of subconfluent monolayers and of [3H]proline into collagenase-sensitive protein. Tritium 157-159 epidermal growth factor like 1 Rattus norvegicus 25-28 6633967-5 1983 The immunoreactive fractions were also active in inhibiting the binding of [3H]enkephalin to rat brain membranes. Tritium 76-78 proenkephalin Rattus norvegicus 79-89 7076122-6 1982 By radiolabeling with [3H]dansyl chloride, two poly(A)-associated proteins with molecular masses of 77,000 Da (P77) and 54,000 Da (P54) were identified. Tritium 23-25 interferon-induced protein with tetratricopeptide repeats 2 Mus musculus 131-134 8206647-3 1994 Epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) added singly caused significant increases relative to control in both the uptake of [3H]thymidine into the cellular DNA of subconfluent monolayers and of [3H]proline into collagenase-sensitive protein. Tritium 227-229 epidermal growth factor like 1 Rattus norvegicus 0-23 8206647-3 1994 Epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) added singly caused significant increases relative to control in both the uptake of [3H]thymidine into the cellular DNA of subconfluent monolayers and of [3H]proline into collagenase-sensitive protein. Tritium 227-229 epidermal growth factor like 1 Rattus norvegicus 25-28 6224755-10 1983 In biochemical experiments, incorporation of [3H]-retinol into mouse rhodopsin was seen to occur very rapidly (less than 30 min), without an appreciable lag period. Tritium 46-48 rhodopsin Mus musculus 69-78 8106564-2 1994 In the Pola mutant x senescent heterodikaryons, both human and murine nuclei display significantly diminished levels of DNA synthesis at the restrictive temperature (39.5 degrees C) as determined by [3H]thymidine labeling in autoradiographs. Tritium 200-202 DNA polymerase alpha 1, catalytic subunit Homo sapiens 7-11 6459382-8 1982 In allogeneic MLTC, "old" T lymphocytes produce little IL-2 compared with "young" cells, and both "young" and "old" cells respond to exogenous IL-2 with enhanced 3H-TdR uptake and increased cytotoxic activity. Tritium 162-164 interleukin 2 Rattus norvegicus 143-147 8113839-6 1994 Two patients with evidence of synergistic stimulation of [3H]-TdR incorporation showed IL7-R gene expression by RT-PCR and IL7 affinity cross-linking. Tritium 58-60 interleukin 7 receptor Homo sapiens 87-92 6798118-9 1982 IL-2 restored the response in B cell cultures depleted of T cells and macrophages, and enhanced 3H-TdR uptake in whole spleen cells of Lewis but not BN rats. Tritium 96-98 interleukin 2 Rattus norvegicus 0-4 6192230-11 1983 Substance P at 100 microM induced a peak release of 2.19% of tissue stores of 3H. Tritium 78-80 tachykinin 1 Mus musculus 0-11 8309245-5 1994 Fourteen of the 18 cases with concomitant rises of 3H-TdR and 3H-araC incorporation into the DNA after GM-CSF had elevated DNA polymerase alpha activity (16-531%, median 72%) and in ten cases overall DNA polymerase activity was enhanced (10-70%, median 22.5%). Tritium 51-53 DNA polymerase alpha 1, catalytic subunit Homo sapiens 123-143 6869531-4 1983 In vivo synthesis of [3H]NE from [3H]dopamine by the enzyme dopamine beta-hydroxylase was 60% lower in IBAT but was equal in hearts of obese compared with lean rats. Tritium 22-24 dopamine beta-hydroxylase Rattus norvegicus 60-85 6344654-7 1983 [3H]LHRH was presented to the renal artery of the filtering rat kidney in vivo, and urine and renal venous blood were analyzed for breakdown products. Tritium 1-3 gonadotropin releasing hormone 1 Rattus norvegicus 4-8 6344654-9 1983 When [3H]LHRH was perfused in vivo through the nonfiltering rat kidney or rat lower limb, renal or femoral venous blood was found to contain only LHRH. Tritium 6-8 gonadotropin releasing hormone 1 Rattus norvegicus 9-13 6344654-10 1983 These studies suggest that [3H]LHRH undergoes glomerular filtration and contact digestion by brush border enzymes of the proximal tubule to produce metabolites 2, 3, and 4. Tritium 28-30 gonadotropin releasing hormone 1 Rattus norvegicus 31-35 7131592-5 1982 However, depolarization-dependent release of [3H]GABA from synaptosomes treated with phospholipase A2 was significantly inhibited. Tritium 45-48 phospholipase A2 Apis mellifera 85-101 6271752-2 1981 The solubilized sodium channel migrated on a sucrose density gradient with an apparent S20,w of approximately 12 S, retained [3H]saxitoxin ([3H]STX) binding activity that was labile at 36 degrees C but no longer bound 125I-labeled scorpion toxin (125I-ScTX). Tritium 141-143 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 Rattus norvegicus 144-147 6271752-3 1981 Following reconstitution into phosphatidylcholine vesicles, the channel regained 125I-ScTX binding and thermal stability of [3H]STX binding. Tritium 125-127 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 Rattus norvegicus 128-131 8309245-5 1994 Fourteen of the 18 cases with concomitant rises of 3H-TdR and 3H-araC incorporation into the DNA after GM-CSF had elevated DNA polymerase alpha activity (16-531%, median 72%) and in ten cases overall DNA polymerase activity was enhanced (10-70%, median 22.5%). Tritium 62-64 DNA polymerase alpha 1, catalytic subunit Homo sapiens 123-143 7888147-0 1994 [3H]paroxetine and [3H]citalopram as markers of the human brain 5-HT uptake site: a comparison study. Tritium 1-3 POU class 6 homeobox 1 Homo sapiens 58-65 6272946-9 1981 The delayed appearance of the [3H]enkephalin-labeled receptors may reflect the dependence of the peptide binding on later developing molecules of adenylate cyclase. Tritium 31-33 proenkephalin Rattus norvegicus 34-44 6882554-3 1983 A difference in AChE activity in the distal and proximal head of the long palmar was observed by autoradiography with 3H-DFP. Tritium 118-120 acetylcholinesterase Rattus norvegicus 16-20 7510997-4 1994 Immunopurification from cytosol of [3H]steroid-labeled tungstate-stabilized PR with anti-PR immunoadsorbent yielded "9S"-PR species in which hsp90, hsp70 and p59/HBI were present. Tritium 36-38 progesterone receptor Homo sapiens 76-78 6300148-6 1983 Quantitation of EGF-stimulated DNA synthesis revealed a decrease in the percentage of cells incorporating [3H]thymidine ([3H]TdR) during a 30-h exposure from 45% in young cells to 0.25% in senescent cells, although [125I]EGF binding or processing-degradation did not differ significantly in young and old cells. Tritium 107-109 epidermal growth factor Homo sapiens 16-19 6300148-6 1983 Quantitation of EGF-stimulated DNA synthesis revealed a decrease in the percentage of cells incorporating [3H]thymidine ([3H]TdR) during a 30-h exposure from 45% in young cells to 0.25% in senescent cells, although [125I]EGF binding or processing-degradation did not differ significantly in young and old cells. Tritium 122-124 epidermal growth factor Homo sapiens 16-19 6132825-9 1983 Moreover, the binding affinity of sufentanil for delta-type binding sites labelled by [3H] [D-Ala2,D-Leu5]enkephalin was found to be 100 times lower than its binding affinity for the mu-receptor sites. Tritium 87-90 proenkephalin Rattus norvegicus 106-116 6847697-8 1983 Furthermore, when SDS-solubilized, [3H]pargyline-labeled MAO A and B proteins from these cell lines were subjected to limited proteolysis and one-dimensional peptide mapping in SDS gels, different patterns of [3H]pargyline-labeled peptides were obtained. Tritium 36-38 monoamine oxidase A Homo sapiens 57-62 6298774-4 1983 In contrast, incorporation of (3)H-labeled amino acids into newly synthesized fibronectin in SAM is not significantly influenced by the transformation process during this time. Tritium 30-34 fibronectin 1 Gallus gallus 78-89 6266515-1 1981 FK-33-824 (Try-D-Ala-Gly-MePhe-Met(O)ol) is a potent enkephalin analog which has been tritium labelled with a high specific radioactivity (41 Ci/mmole). Tritium 86-93 proenkephalin Rattus norvegicus 53-63 6945896-6 1981 The kinetic disappearance of [3H]chol-VLDL and its remnants in the perfused liver system indicated that remnant of d less than 1.019 g. mL-1 was taken up by the liver faster than the original VLDL preparation (t1/2 of 8 min vs. 51 min). Tritium 30-32 L1 cell adhesion molecule Mus musculus 136-140 6783656-14 1981 In contrast, lysine 108, which is not sufficiently close to the interaction site to be perturbed in differential labeling studies but is greatly decreased in tritium content in the cross-linked complex, appears to represent the major site through which alpha-lactalbumin is cross-linked to galactosyltransferase as a result of the exclusion of protein molecules acetylated in this position from covalent cross-linking. Tritium 158-165 lactalbumin alpha Bos taurus 253-270 7225408-2 1981 Solutions of different ratios of alpha 1 and alpha 2 chains, one of which was labelled with [14C]- or [3H]glycine, were renatured to form triple-helical, rod-shaped molecules, then cross-linked intramolecularly with formaldehyde. Tritium 103-105 adrenoceptor alpha 1D Homo sapiens 33-52 6302675-1 1983 The regulation of mu- and delta-opiate receptors by guanine nucleotides and cations was studied by examining the binding of [3H][DAla2, DLeu5]enkephalin to rat brain membranes. Tritium 125-127 proenkephalin Rattus norvegicus 142-152 7510997-4 1994 Immunopurification from cytosol of [3H]steroid-labeled tungstate-stabilized PR with anti-PR immunoadsorbent yielded "9S"-PR species in which hsp90, hsp70 and p59/HBI were present. Tritium 36-38 progesterone receptor Homo sapiens 89-91 7214150-7 1981 [3H]mianserin interactions with 5-HT2 sites are studied selectively by displacing histamine H1-receptor binding with the H1-antihistamine triprolidine. Tritium 1-3 histamine receptor H1 Homo sapiens 82-103 7510997-4 1994 Immunopurification from cytosol of [3H]steroid-labeled tungstate-stabilized PR with anti-PR immunoadsorbent yielded "9S"-PR species in which hsp90, hsp70 and p59/HBI were present. Tritium 36-38 progesterone receptor Homo sapiens 89-91 6652985-8 1983 3H content remained higher in arteries of SHR than in those of WKY but after catechol-O-methyltransferase (COMT) was inhibited by U-0521, the difference was not significant. Tritium 0-2 catechol-O-methyltransferase Rattus norvegicus 107-111 7505392-4 1994 Spheroplasts expressing wild-type Mdr3 (Ser-939) exhibit an ATP-dependent and verapamil-sensitive decreased accumulation of [3H]vinblastine as compared with spheroplasts expressing a mutant form of Mdr3 with impaired transport activity (Phe-939). Tritium 125-127 ATP-binding cassette, sub-family B (MDR/TAP), member 1A Mus musculus 34-38 6323709-3 1983 The order of agonist competition for the [3H]-DHA binding site of DDT1 cell membranes was isoproterenol (Ki = 0.20 +/- 0.07 microM) greater than epinephrine (Ki = 0.4 +/- 0.2 microM) greater than norepinephrine (Ki = 66.5 +/- 5.15 microM) consistent with a beta 2-adrenergic receptor interaction. Tritium 42-44 beta-2 adrenergic receptor Mesocricetus auratus 257-283 6259878-2 1981 The 3H-labeled OT with adequate specific activity (24 Ci/mM) and biologic activity (350 IU/mg) was employed for the OT radioreceptor assay and the apparent dissociation constant (Kds) and the number of binding sites (NBS) were analyzed by Scatchard plot. Tritium 4-6 nibrin Homo sapiens 217-220 6785376-1 1981 The tyrosinase activity of Himalayan mouse skin homogenates was measured over a range of temperatures using two sensitive radiometric assay--namely, (1) the measurement of 14C-tyrosine incorporation into melanin, and (2) the measurement of 3HOH released as a by-product of 3H-tyrosine hydroxylation. Tritium 240-242 tyrosinase Mus musculus 4-14 8186462-3 1994 However, such 3-kinase-transfected cells, when labeled with [3H]inositol for 48-72 h, showed lower levels of [3H]InsP5 and [3H]InsP6, as well as [3H]Ins(1,3,4,6)P4 and D/L[3H]Ins(1,4,5,6)P4, than their vector-transfected counterparts. Tritium 61-63 inositol hexaphosphate kinase 1 Mus musculus 127-132 7002926-2 1980 Primary tritium isotope effects have been measured for the hydroxylation of [2-3H]dopamine catalyzed by dopamine beta-hydroxylase. Tritium 8-15 dopamine beta-hydroxylase Homo sapiens 104-129 7161327-8 1982 Maximum inhibition of [3H]Leu-Enk degradation is obtained at a concentration of 10(-3) M Phe-Ala. Tritium 23-25 proenkephalin Rattus norvegicus 30-33 6749839-6 1982 Fibronectin and collagen were detected on the bases of their relative mobilities, differential labeling with 3H-versus 35S-labeled amino acids and their solubilization by protease free collagenase. Tritium 109-111 fibronectin 1 Mus musculus 0-11 8297925-1 1993 The effects of 5-HT1A antagonists spiperone, methiothepin and BMY 7378 on [3H]-8-OH-DPAT binding were determined in vitro and ex vivo in rat hippocampus CA3 membrane preparations, and ex vivo in tissue sections of CA1 and CA3 subfields using quantitative autoradiography. Tritium 75-77 5-hydroxytryptamine receptor 1A Rattus norvegicus 15-21 7118891-5 1982 In the presence of cycloheximide such that VLDL apoprotein synthesis is inhibited 98%, the secretion of lipids labeled from a [3H]palmitate pulse by hepatocyte monolayers was halted only after completed apoprotein chains had cleared the cell. Tritium 127-129 very low density lipoprotein receptor Gallus gallus 43-47 7450912-0 1980 Tritium production in LiF thermoluminescent dosimeters. Tritium 0-7 LIF interleukin 6 family cytokine Homo sapiens 22-25 8282003-1 1993 [3H]SR 47436, a selective and potent novel non-peptide antagonist of angiotensin receptors, was used to characterize the cardiac angiotensin AT1 receptors. Tritium 1-3 angiotensin II receptor, type 1a Rattus norvegicus 141-144 6286709-6 1982 Stimulation of [3H] aminoisobutyric acid uptake by insulin, epidermal growth factor (EGF), multiplication-stimulating activity, and somatomedin C (Sm-C) in Leprechaun cells was diminished relative to control cells at all concentrations tested. Tritium 16-18 epidermal growth factor Homo sapiens 85-88 6286709-7 1982 Furthermore, stimulation of [3H]thymidine incorporation in Leprechaum cells by EGF, Sm-C, and fibroblast growth factor was also subnormal. Tritium 29-31 epidermal growth factor Homo sapiens 79-82 6286683-3 1982 32Pi introduced into MEP by metabolic labeling of intact cells is exclusively associated with asparagine-linked oligosaccharides as indicated by sensitivity to endohexosaminidase H. Labeling studies utilizing [2-3H]mannose indicate that approximately one-fifth of the mannose residues of MEP are phosphorylated. Tritium 212-214 cathepsin L Mus musculus 21-24 6809819-1 1982 T cell and interleukin 2 (IL 2) regulation of two phases of B cell activation, 3H-TdR uptake and differentiation to antibody-forming cells, were examined. Tritium 79-81 interleukin 2 Mus musculus 26-30 7462205-3 1980 [3H]E-64 is incorporated into cathepsin B in a one/one molar ratio in parallel with inactivation of the enzyme. Tritium 1-3 cathepsin B Rattus norvegicus 30-41 6450610-2 1980 Rhodopsin containing [11-3H]retinal was prepared in chromatographically pure form, and the [3H]retinal was reductively linked to its binding site on opsin by using borane--dimethylamine. Tritium 25-27 rhodopsin Bos taurus 0-9 6450610-2 1980 Rhodopsin containing [11-3H]retinal was prepared in chromatographically pure form, and the [3H]retinal was reductively linked to its binding site on opsin by using borane--dimethylamine. Tritium 92-94 rhodopsin Bos taurus 0-9 6450610-5 1980 The retinyl binding site is located in the carboxyl-terminal region of rhodopsin: when rod cell disk membranes containing [3H]retinal rhodopsin were digested with thermolysin and then reacted with sodium borohydride or borane--dimethylamine, [3H]retinal was reduced onto the F2 (Mr congruent to 6000) fragment, which derives from rhodopsin"s carboxyl-terminal region. Tritium 123-125 rhodopsin Bos taurus 71-80 6450610-5 1980 The retinyl binding site is located in the carboxyl-terminal region of rhodopsin: when rod cell disk membranes containing [3H]retinal rhodopsin were digested with thermolysin and then reacted with sodium borohydride or borane--dimethylamine, [3H]retinal was reduced onto the F2 (Mr congruent to 6000) fragment, which derives from rhodopsin"s carboxyl-terminal region. Tritium 123-125 rhodopsin Bos taurus 134-143 6450610-5 1980 The retinyl binding site is located in the carboxyl-terminal region of rhodopsin: when rod cell disk membranes containing [3H]retinal rhodopsin were digested with thermolysin and then reacted with sodium borohydride or borane--dimethylamine, [3H]retinal was reduced onto the F2 (Mr congruent to 6000) fragment, which derives from rhodopsin"s carboxyl-terminal region. Tritium 123-125 rhodopsin Bos taurus 134-143 6450610-5 1980 The retinyl binding site is located in the carboxyl-terminal region of rhodopsin: when rod cell disk membranes containing [3H]retinal rhodopsin were digested with thermolysin and then reacted with sodium borohydride or borane--dimethylamine, [3H]retinal was reduced onto the F2 (Mr congruent to 6000) fragment, which derives from rhodopsin"s carboxyl-terminal region. Tritium 243-245 rhodopsin Bos taurus 71-80 6286904-6 1982 Visualization of [3H]naloxone- or [3H]enkephalin-labeled opiate receptor distributions in rat and human brains is achieved by tritium-sensitive film or by classical "wet" emulsion autoradiography. Tritium 126-133 proenkephalin Rattus norvegicus 38-48 8253120-5 1993 An identical distribution of specific binding was detected in adjacent sections incubated with the selective 5-HT3 receptor radioligand, [3H](S)-zacopride (0.5 nM; non-specific binding defined by the inclusion of granisetron, 1.0 microM). Tritium 138-140 5-hydroxytryptamine receptor 3A Rattus norvegicus 109-123 7106368-8 1982 This induced dissociation allows the complete dissociation of estrogen receptor-[3H]-ligand complexes in a short time (less than 24 h) at low temperatures without alteration of the level of [3H] ligand bound non-specifically and can therefore be used to measure the [3H] ligand bound to the receptor by exchange at 0-4 degree C. From the specificity and the high doses of inducers required to make possible the observation of a significant effect, we conclude that the induced dissociation probably does not have a biological role. Tritium 81-83 estrogen receptor 1 Rattus norvegicus 62-79 6997310-1 1980 The steric course of the methyl group transfer catalyzed by catechol O-methyltransferase was studied using S-adenosylmethionine (AdoMet) carrying a methyl group made chiral by labeling with 1H, 2H, and 3H in an asymmetrical arrangement. Tritium 202-204 catechol-O-methyltransferase Rattus norvegicus 60-88 7398581-4 1980 Term placental sections were incubated with [3H]cDNA transcribed from sucrose gradient-purified hPL mRNA. Tritium 45-47 galectin 1 Homo sapiens 96-99 7106368-8 1982 This induced dissociation allows the complete dissociation of estrogen receptor-[3H]-ligand complexes in a short time (less than 24 h) at low temperatures without alteration of the level of [3H] ligand bound non-specifically and can therefore be used to measure the [3H] ligand bound to the receptor by exchange at 0-4 degree C. From the specificity and the high doses of inducers required to make possible the observation of a significant effect, we conclude that the induced dissociation probably does not have a biological role. Tritium 191-193 estrogen receptor 1 Rattus norvegicus 62-79 8253120-8 1993 These studies indicate that [3H]mCPBG labels the 5-HT3 receptor in rat brain tissue. Tritium 29-31 5-hydroxytryptamine receptor 3A Rattus norvegicus 49-63 7106368-8 1982 This induced dissociation allows the complete dissociation of estrogen receptor-[3H]-ligand complexes in a short time (less than 24 h) at low temperatures without alteration of the level of [3H] ligand bound non-specifically and can therefore be used to measure the [3H] ligand bound to the receptor by exchange at 0-4 degree C. From the specificity and the high doses of inducers required to make possible the observation of a significant effect, we conclude that the induced dissociation probably does not have a biological role. Tritium 191-193 estrogen receptor 1 Rattus norvegicus 62-79 8281995-1 1993 Neuropeptide Y (NPY-(1-36)), peptide YY (PYY) and various other peptides were investigated for their interaction with the binding of [3H](+)-SKF10,047 to sigma binding sites in mouse hippocampus in vivo. Tritium 134-136 neuropeptide Y Mus musculus 0-14 7110115-3 1982 In contrast, Scatchard analysis of the binding of [3H]leucine enkephalin in the absence and presence of 10, 50, and 100 nM morphine demonstrated that these concentrations of morphine decreased the number of binding sites by 23%, 32%, and 42%, respectively, with no change in the KD. Tritium 51-54 proenkephalin Rattus norvegicus 62-72 7398581-7 1980 The amount of this radioactivity was greatly reduced when placental fragments were incubated with globin [3H]cDNA or if hPL [3H]cDNA was hybridized to sections of human tonsil tissue. Tritium 125-127 galectin 1 Homo sapiens 120-123 6933473-5 1980 Sequence analysis of each CNBr fragment intrinsically labeled with 3H- and 35S-labeled amino acids identified a total of 87 residues in the H-2Kk heavy chain. Tritium 67-69 histocompatibility 2, K1, K region Mus musculus 140-145 8281995-1 1993 Neuropeptide Y (NPY-(1-36)), peptide YY (PYY) and various other peptides were investigated for their interaction with the binding of [3H](+)-SKF10,047 to sigma binding sites in mouse hippocampus in vivo. Tritium 134-136 neuropeptide Y Mus musculus 16-19 7110115-7 1982 Computer analysis of the displacement [3H]leucine enkephalin binding by various concentrations of morphine has demonstrated that an allosteric model, not a two-site model, best describes the observed data. Tritium 39-41 proenkephalin Rattus norvegicus 50-60 8399136-8 1993 Saturation binding analysis showed that while the presence of any of the dihydroxyl ligands or that of estratrien-17 beta-ol decreased the positive cooperativity of the [3H]estradiol-estrogen receptor interaction, the presence of the 3-hydroxyestratriene ligand increased it. Tritium 170-172 estrogen receptor 1 Bos taurus 183-200 6287305-5 1982 However, in saturation binding studies, performed in diencephalic membranes, in which [D-Ala2,D-Leu5]-enkephalin binds predominantly to mu-receptors, Ca2+ also increased the binding affinity of [3H]- [D-Ala2,D-Leu5]-enkephalin. Tritium 195-197 proenkephalin Rattus norvegicus 102-112 6287305-5 1982 However, in saturation binding studies, performed in diencephalic membranes, in which [D-Ala2,D-Leu5]-enkephalin binds predominantly to mu-receptors, Ca2+ also increased the binding affinity of [3H]- [D-Ala2,D-Leu5]-enkephalin. Tritium 195-197 proenkephalin Rattus norvegicus 216-226 7060000-1 1982 The binding of tritium-labeled 5-methylchrysene to DNA of CD-1 mouse skin 24 hr after treatment has been studied. Tritium 15-22 CD1 antigen complex Mus musculus 58-62 6121002-2 1982 [3H]PLG and metabolites were determined in trichloroacetic acid extracts by reverse-phase paired-ion HPLC. Tritium 1-3 plasminogen Rattus norvegicus 4-7 6121002-4 1982 The uptake of [3H]PLG in the brain ranged from 0.0013% to 0.0017% of the administered dose per g tissue at 6-30 min following subcutaneous injection. Tritium 15-17 plasminogen Rattus norvegicus 18-21 6121002-5 1982 After comparing these results with our previous findings with intravenous injection of [3H]PLG, it seemed likely that the subcutaneous route of administration might be more effective in eliciting CNS effects of PLG than the intravenous route of administration. Tritium 88-90 plasminogen Rattus norvegicus 91-94 6121002-5 1982 After comparing these results with our previous findings with intravenous injection of [3H]PLG, it seemed likely that the subcutaneous route of administration might be more effective in eliciting CNS effects of PLG than the intravenous route of administration. Tritium 88-90 plasminogen Rattus norvegicus 211-214 6245752-1 1980 The ontogenesis of opiate receptors in several brain regions of the developing rat was measured by specific binding of [3H]methionine-enkephalin ([3H]Met). Tritium 120-122 proenkephalin Rattus norvegicus 134-144 6245752-1 1980 The ontogenesis of opiate receptors in several brain regions of the developing rat was measured by specific binding of [3H]methionine-enkephalin ([3H]Met). Tritium 147-149 proenkephalin Rattus norvegicus 134-144 230477-4 1979 Similar to their effect on 3H-labeled agonist binding, Na+ and other monovalent cations, GTP, trypsin, chymotrypsin, phospholipase A2, and N-ethylmaleimide all inhibited the specific binding of beta h-[3H]endorphin. Tritium 27-29 phospholipase A2 group IB Rattus norvegicus 117-133 8258357-5 1993 Recombinant receptors consisting of beta 2 and gamma 2 subunits together with the wildtype alpha 6 or mutant alpha 6(Q100) subunit showed positive modulation of [3H]Ro 15-4513 binding by GABA, whereas alpha 1 beta 2 gamma 2 receptors showed negative modulation. Tritium 162-164 crystallin, gamma E Rattus norvegicus 47-54 447724-1 1979 The sugar chains of bovine rhodopsin were released from the polypeptide moiety by hydrazinolysis and reduced with NaB[3H]4 after N-acetylation. Tritium 118-120 rhodopsin Bos taurus 27-36 476785-2 1979 A low level incorporation of [3H]TdT into the nuclear DNA of Purkinje neurons was found in autoradiographs. Tritium 30-32 deoxynucleotidyltransferase, terminal Mus musculus 33-36 6953412-3 1982 Furthermore, in the presence of Mg2+, eIF-2-SP catalyzes the exchange of eIF-2-bound [3H]GDP with unlabeled GDP or GTP (GDP exchange) and the release of [3H]GDP when the ternary complex is formed from eIF-2-[3H]GDP, GTP, and [35S]Met-tRNAi. Tritium 86-88 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 38-46 6953412-3 1982 Furthermore, in the presence of Mg2+, eIF-2-SP catalyzes the exchange of eIF-2-bound [3H]GDP with unlabeled GDP or GTP (GDP exchange) and the release of [3H]GDP when the ternary complex is formed from eIF-2-[3H]GDP, GTP, and [35S]Met-tRNAi. Tritium 86-88 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 38-43 6953412-3 1982 Furthermore, in the presence of Mg2+, eIF-2-SP catalyzes the exchange of eIF-2-bound [3H]GDP with unlabeled GDP or GTP (GDP exchange) and the release of [3H]GDP when the ternary complex is formed from eIF-2-[3H]GDP, GTP, and [35S]Met-tRNAi. Tritium 86-88 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 73-78 7688745-6 1993 Treatment with alpha 2M-MA or TGF-beta 1 stimulated a 3- or 3.5-fold increase in [3H]thymidine incorporation, respectively. Tritium 82-84 alpha-2-macroglobulin Rattus norvegicus 15-23 6953412-3 1982 Furthermore, in the presence of Mg2+, eIF-2-SP catalyzes the exchange of eIF-2-bound [3H]GDP with unlabeled GDP or GTP (GDP exchange) and the release of [3H]GDP when the ternary complex is formed from eIF-2-[3H]GDP, GTP, and [35S]Met-tRNAi. Tritium 154-156 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 38-46 6953412-3 1982 Furthermore, in the presence of Mg2+, eIF-2-SP catalyzes the exchange of eIF-2-bound [3H]GDP with unlabeled GDP or GTP (GDP exchange) and the release of [3H]GDP when the ternary complex is formed from eIF-2-[3H]GDP, GTP, and [35S]Met-tRNAi. Tritium 154-156 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 38-43 6953412-3 1982 Furthermore, in the presence of Mg2+, eIF-2-SP catalyzes the exchange of eIF-2-bound [3H]GDP with unlabeled GDP or GTP (GDP exchange) and the release of [3H]GDP when the ternary complex is formed from eIF-2-[3H]GDP, GTP, and [35S]Met-tRNAi. Tritium 154-156 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 73-78 108280-2 1979 We now present evidence that these fluctuations result from adaptations intrinsic to adipose tissue glycogen metabolism that persist in vitro: in response to insulin (1 milliunit/ml), [3H]glucose incorporation into rat fat pad glycogen was reduced to 10% of control after a 3-day fast; incorporation increased 6-fold over fed control on the 4th day of refeeding following a 3-day fast. Tritium 185-187 insulin 1 Rattus norvegicus 158-168 6953412-3 1982 Furthermore, in the presence of Mg2+, eIF-2-SP catalyzes the exchange of eIF-2-bound [3H]GDP with unlabeled GDP or GTP (GDP exchange) and the release of [3H]GDP when the ternary complex is formed from eIF-2-[3H]GDP, GTP, and [35S]Met-tRNAi. Tritium 154-156 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 38-46 7688745-7 1993 Cotreatment with TGF-beta 1 and alpha 2M-MA resulted in a 70-fold increase in [3H]thymidine incorporation. Tritium 79-81 alpha-2-macroglobulin Rattus norvegicus 32-40 8350385-6 1993 Maximal reduction in Bmax of [3H]QNB and [3H]-4-DAMP binding occurred in mice with highest AChE inhibition, while equivalent maximal reduction in Bmax of [3H]NMS occurred in mice with only approximately 10% AChE inhibition, without further change at higher parathion doses. Tritium 30-32 acetylcholinesterase Mus musculus 91-95 6279240-4 1982 The specific [3H]muscimol binding to the soluble fraction obtained by Nonidet P-40 treatment and subsequent ammonium sulfate precipitation, was saturable with KD 13 and 64 nM, and Bmax 3.4 and 1.8 pmol/mg protein, respectively. Tritium 14-16 septin 3 Rattus norvegicus 78-82 92923-4 1979 3.--After intracisternally injection of [14C]DA or [3H]NA, SAH increases the level of [14C]MD and [3H]NM. Tritium 52-54 acyl-CoA synthetase medium-chain family member 3 Rattus norvegicus 59-62 8234033-1 1993 We have investigated the intracellular mechanisms underlying thyrotropin-releasing hormone (TRH)-mediated [3H]dopamine ([3H]DA) release from dispersed rat tuberoinfundibular dopaminergic (TIDA) neurons. Tritium 107-109 thyrotropin releasing hormone Rattus norvegicus 61-90 111806-1 1979 The effects of various inhibitors of aryl hydrocarbon hydroxylase (AHH), antioxidants, inhibitors of DNA, RNA, and protein synthesis, and protease inhibitors on the binding of [7,12-3H]dimethylbenz[a]anthracene ([3H] DMBA) to DNA of murine epidermal cells in culture have been investigated. Tritium 182-184 cytochrome P450, family 1, subfamily a, polypeptide 1 Mus musculus 37-65 469850-1 1979 The synthesis of a soluble protein (referred to as "decidualization-associated protein", DAP), has been examined in uterine and placental tissues of rats during pregnancy by means of polyacrylamide gel electrophoretic analysis of [3H]leucine-labelled soluble proteins. Tritium 231-233 death-associated protein Rattus norvegicus 89-92 7072716-1 1982 Recombinant plasmid HCS-pBR322 containing a 550-base-pair (bp) insert of cDNA to human placental lactogen (hPL) mRNA was 3H-labeled by nick translation and hybridized in situ to human chromosome preparations in the presence of 10% dextran sulfate. Tritium 121-123 galectin 1 Homo sapiens 107-110 6290171-4 1982 [3H]-dihydroalprenolol binding was significantly reduced in weaver, reeler, and staggerer (14-22% of control values) at 20 days, but not in the jimpy mutant. Tritium 1-3 RAR-related orphan receptor alpha Mus musculus 80-89 7087686-2 1982 In the first series of experiments involving the utilization as substrates of PI with different 14C- or 3H-labeled fatty acids in the 2-position, the purified phospholipase A2 most readily removed 16:0 palmitic acid, followed by 18:0 stearic acid, 18:1 oleic acid and 20:4 arachidonic acid. Tritium 104-106 LOC104974671 Bos taurus 159-175 8234033-1 1993 We have investigated the intracellular mechanisms underlying thyrotropin-releasing hormone (TRH)-mediated [3H]dopamine ([3H]DA) release from dispersed rat tuberoinfundibular dopaminergic (TIDA) neurons. Tritium 107-109 thyrotropin releasing hormone Rattus norvegicus 92-95 220063-5 1979 [3H]Neurotensin bound specifically to membranes prepared from isolated longitudinal muscle strips of the guinea-pig intestine. Tritium 1-3 neurotensin/neuromedin N Cavia porcellus 4-15 220063-6 1979 In binding studies using [3H]neurotensin and unlabelled peptides, the KDs of neurotensin, D-Arg 8-neurotensin and D-Arg9 neurotensin were 4 nM, 2 nM and 300 nM, respectively, values which are in close agreement with the EC50 of the three peptides. Tritium 26-28 neurotensin/neuromedin N Cavia porcellus 29-40 8364729-3 1993 Stress, but not antidepressant, depressed 5-HT1A sites labelled with [3H]8-hydroxy-DPAT in hippocampal fields CA3, CA4 and dentate gyrus. Tritium 70-72 5-hydroxytryptamine receptor 1A Rattus norvegicus 42-48 220063-6 1979 In binding studies using [3H]neurotensin and unlabelled peptides, the KDs of neurotensin, D-Arg 8-neurotensin and D-Arg9 neurotensin were 4 nM, 2 nM and 300 nM, respectively, values which are in close agreement with the EC50 of the three peptides. Tritium 26-28 neurotensin/neuromedin N Cavia porcellus 77-120 6288583-3 1982 [delta Ala2, Leu5]-enkephalin has been prepared and shown to be more active than the parent saturated enkephalin in a binding assay using rat brain membranes and [3H]dihydromorphine as a tracer. Tritium 163-165 proenkephalin Rattus norvegicus 19-29 220063-6 1979 In binding studies using [3H]neurotensin and unlabelled peptides, the KDs of neurotensin, D-Arg 8-neurotensin and D-Arg9 neurotensin were 4 nM, 2 nM and 300 nM, respectively, values which are in close agreement with the EC50 of the three peptides. Tritium 26-28 neurotensin/neuromedin N Cavia porcellus 77-88 8514774-5 1993 The methyl group is not stable on the protein and is lost as [3H]methanol with a half-life of about 180 min at pH 7.0, 37 degrees C. The methyltransferase responsible for this reaction is a cytosolic protein with a native molecular mass of about 40 kDa that is readily separated from the well described protein-L-isoaspartate (D-aspartate) O-methyltransferase (EC 2.1.1.77). Tritium 62-64 protein-L-isoaspartate (D-aspartate) O-methyltransferase Bos taurus 303-359 6952216-4 1982 The concentration of this immunoreactive protein in the cytoplasm of human leukemia and normal leukocytes in all instances greatly exceeded the concentration of functional dihydrofolate reductase, which was measured by the binding of [3H]methotrexate. Tritium 235-237 dihydrofolate reductase Homo sapiens 172-195 8388511-6 1993 9E3/CEF4 was slightly mitogenic for CEF, causing a doubling of [3H]thymidine uptake when added to serum-starved CEF.9E3/CEF4 was found associated not only with the cell and in the culture medium of Rous sarcoma virus-transformed CEF but also with the extracellular matrix. Tritium 64-66 interleukin 8-like 2 Gallus gallus 4-8 7309750-11 1981 Proteolytic fragmentation of [3H]mannose-labeled beta-glucuronidase and partial digestion of [3H]leucine-labeled beta-glucuronidase with endo-beta-N-acetylglucosaminidase H suggest that there are 3 glycosylation sites per subunit. Tritium 30-32 glucuronidase, beta Mus musculus 49-67 7309750-11 1981 Proteolytic fragmentation of [3H]mannose-labeled beta-glucuronidase and partial digestion of [3H]leucine-labeled beta-glucuronidase with endo-beta-N-acetylglucosaminidase H suggest that there are 3 glycosylation sites per subunit. Tritium 94-96 glucuronidase, beta Mus musculus 113-131 7310821-7 1981 The rank order of displacement of [3H]APO by potent aporphines (IC50 less than or equal to 30 nM) correlated approximately with their ability to stimulate cyclic AMP synthesis. Tritium 35-37 aminopeptidase O Rattus norvegicus 38-41 218679-8 1979 The data presented strongly support the proposal that the measurement of [3H]GABA binding in postmortem human brain offers a reflection of the state of the physiologically relevant GABA receptor. Tritium 74-76 GABA type A receptor-associated protein Homo sapiens 181-194 446374-6 1979 The only detectable somatomedin activity (measured by [3H]thymidine incorporation into chick embryo fibroblast DNA) in fractions of normal rat serum chromatographed on Sephadex G-200 coincides with peak II binding of [125I]iodo-MSA. Tritium 55-57 insulin like growth factor 1 Gallus gallus 20-31 8388511-6 1993 9E3/CEF4 was slightly mitogenic for CEF, causing a doubling of [3H]thymidine uptake when added to serum-starved CEF.9E3/CEF4 was found associated not only with the cell and in the culture medium of Rous sarcoma virus-transformed CEF but also with the extracellular matrix. Tritium 64-66 interleukin 8-like 2 Gallus gallus 120-124 8392757-5 1993 The working hypothesis was that if carfentanil was acting through another high-affinity site besides the mu opiate receptor, the distribution pattern of the autoradiographic image produced by [3H]carfentanil should be significantly different than the autoradiographic pattern displayed by the well-characterized and selective mu opiate [3H]DAMGO. Tritium 193-195 opioid related nociceptin receptor 1 Rattus norvegicus 105-123 721806-3 1978 After [3H]ADP-ribosylation by [3H]nad+ and diphtheria toxin, EF-2 was digested with trypsin and a homogeneous [3H]ADP-ribosyl peptide was isolated by chromatography on DEAE-Sephadex and dihydroxyboryl-substituted cellulose. Tritium 7-9 elongation factor 2 Saccharomyces cerevisiae S288C 61-65 6271191-4 1981 MP-2 and MP-3, but not MP-1, bound [3H]fMet-Leu-Phe in equilibrium dialysis chambers. Tritium 36-38 tryptase pseudogene 1 Homo sapiens 0-13 8392757-5 1993 The working hypothesis was that if carfentanil was acting through another high-affinity site besides the mu opiate receptor, the distribution pattern of the autoradiographic image produced by [3H]carfentanil should be significantly different than the autoradiographic pattern displayed by the well-characterized and selective mu opiate [3H]DAMGO. Tritium 337-339 opioid related nociceptin receptor 1 Rattus norvegicus 105-123 6895327-10 1981 The interaction of heparin with lipoprotein lipase was studied using a specific [3H]heparin, which was isolated by affinity chromatography on immobilized lipoprotein lipase; the [3H]heparin eluted with 0.6 M NaCl. Tritium 81-83 lipoprotein lipase Homo sapiens 32-50 6895327-10 1981 The interaction of heparin with lipoprotein lipase was studied using a specific [3H]heparin, which was isolated by affinity chromatography on immobilized lipoprotein lipase; the [3H]heparin eluted with 0.6 M NaCl. Tritium 81-83 lipoprotein lipase Homo sapiens 154-172 6895327-10 1981 The interaction of heparin with lipoprotein lipase was studied using a specific [3H]heparin, which was isolated by affinity chromatography on immobilized lipoprotein lipase; the [3H]heparin eluted with 0.6 M NaCl. Tritium 179-181 lipoprotein lipase Homo sapiens 32-50 6895327-11 1981 Specific [3H]heparin coeluted with lipoprotein lipase when the enzyme was associated with phospholipid; the [3H]heparin was released from the enzyme by 0.75 M NaCl. Tritium 10-12 lipoprotein lipase Homo sapiens 35-53 721806-3 1978 After [3H]ADP-ribosylation by [3H]nad+ and diphtheria toxin, EF-2 was digested with trypsin and a homogeneous [3H]ADP-ribosyl peptide was isolated by chromatography on DEAE-Sephadex and dihydroxyboryl-substituted cellulose. Tritium 31-33 elongation factor 2 Saccharomyces cerevisiae S288C 61-65 721806-3 1978 After [3H]ADP-ribosylation by [3H]nad+ and diphtheria toxin, EF-2 was digested with trypsin and a homogeneous [3H]ADP-ribosyl peptide was isolated by chromatography on DEAE-Sephadex and dihydroxyboryl-substituted cellulose. Tritium 31-33 elongation factor 2 Saccharomyces cerevisiae S288C 61-65 9762146-4 1978 Nuclease levels were monitored by coincubating [3H]-labeled Escherichia coli ribosomal RNA with the experimental RNA samples. Tritium 48-50 nuclease Escherichia coli 0-8 8518231-3 1993 EGF and TGF-alpha stimulated uptake of [3H]-thymidine in cultured cytotrophoblasts from first and second trimester placentae, demonstrating both functional EGFR and the mitogenic ability of either growth factor in these cells. Tritium 40-42 transforming growth factor alpha Homo sapiens 8-17 84755-10 1978 The increase in tissue to serum ratios of [3H]estradiol during postnatal development probably resulted from the decline in serum EBP, since injection of neonatal serum into 28-day-old rats reduced tissue to serum ratios of [3H]estradiol to levels which were similar to those observed in 16-day-old animals. Tritium 224-226 EBP, cholestenol delta-isomerase Rattus norvegicus 129-132 6795966-0 1981 The use of a tritium release assay to measure 6-N-trimethyl-L-lysine hydroxylase activity: synthesis of 6-N-[3-3H]trimethyl-DL-lysine. Tritium 13-20 procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 Homo sapiens 62-80 8360278-4 1993 In vitro [3H]fucose incorporation studies combined with in vivo light and electron microscopic autoradiographic analysis, showed that the IRBP-like glycoprotein is synthesized by the neural retina and secreted into the interphotoreceptor matrix. Tritium 10-12 retinol binding protein 3 Homo sapiens 138-142 6457976-0 1981 Specific desensitization of histamine H1 receptor-mediated [3H]glycogen hydrolysis in brain slices. Tritium 60-62 histamine receptor H1 Homo sapiens 28-49 369847-1 1978 In the first experiment, the influence of synthetic gonadotropin-releasing hormone (GnRH) on the time course of [3H]glucosamine ([3H]GA) and [14C]alanine ([14C]A) incorporation into LH by quartered rat anterior pituitary glands and the sequence of release of radiolabeled and total immunoreactive LH (IR-LH) was investigated. Tritium 113-115 gonadotropin releasing hormone 1 Rattus norvegicus 52-82 369847-1 1978 In the first experiment, the influence of synthetic gonadotropin-releasing hormone (GnRH) on the time course of [3H]glucosamine ([3H]GA) and [14C]alanine ([14C]A) incorporation into LH by quartered rat anterior pituitary glands and the sequence of release of radiolabeled and total immunoreactive LH (IR-LH) was investigated. Tritium 113-115 gonadotropin releasing hormone 1 Rattus norvegicus 84-88 369847-1 1978 In the first experiment, the influence of synthetic gonadotropin-releasing hormone (GnRH) on the time course of [3H]glucosamine ([3H]GA) and [14C]alanine ([14C]A) incorporation into LH by quartered rat anterior pituitary glands and the sequence of release of radiolabeled and total immunoreactive LH (IR-LH) was investigated. Tritium 130-132 gonadotropin releasing hormone 1 Rattus norvegicus 52-82 369847-1 1978 In the first experiment, the influence of synthetic gonadotropin-releasing hormone (GnRH) on the time course of [3H]glucosamine ([3H]GA) and [14C]alanine ([14C]A) incorporation into LH by quartered rat anterior pituitary glands and the sequence of release of radiolabeled and total immunoreactive LH (IR-LH) was investigated. Tritium 130-132 gonadotropin releasing hormone 1 Rattus norvegicus 84-88 369847-3 1978 After lag periods of 5 and 20 min, respectively, incorporation of [14C]A and [3H]GA into tissue LH increased linearly for 4 h. GnRH stimulated incorporation of [3H]GA only. Tritium 161-163 gonadotropin releasing hormone 1 Rattus norvegicus 127-131 6942054-3 1981 The effects of detergents on the size of shed I-Ak and H-2Kk and the coprecipitation of (3H) lipids with these proteins suggest that I-Ak and H-2Kk are shed in association with local membrane lipids as small membrane-derived vesicles, whereas membrane IgM is shed without detectable associated lipid. Tritium 89-91 histocompatibility 2, K1, K region Mus musculus 142-147 8390625-11 1993 Finally, we report a loss in the ability of 8-bromo-cyclic AMP- or 8-bromo-cyclic GMP-treated cells to fully induce the activity of phenylethanolamine N-methyltransferase in response to cortisol, indicating that decreases in soluble [3H]dexamethasone binding translate into a decrease in the functional consequence of glucocorticoid receptor binding in adrenal medullary cells. Tritium 234-236 phenylethanolamine N-methyltransferase Bos taurus 132-170 699918-5 1978 The conformational stability of rhodopsin in these two detergents is also demonstrated by the similarity of the tritium exchange-out kinetics and the infrared amide I band frequencies for both membrane-bound and detergent-solubilised rhodopsin. Tritium 112-119 rhodopsin Bos taurus 32-41 7264664-2 1981 [3H]Pargyline was bound to MAO A in a crude mitochondrial fraction from the placental trophoblast of a male newborn and to MAO B in blood platelets from the umbilical vein of the same newborn. Tritium 1-3 monoamine oxidase A Homo sapiens 27-32 8390115-2 1993 Angiotensin converting enzyme (ACE) activity was determined in confluent monolayers, under first-order reaction conditions, at 6, 24, 48, and 96 hr after treatment, using [3H]benzoyl-Phe-Ala-Pro as substrate. Tritium 172-174 angiotensin I converting enzyme Bos taurus 0-29 7264664-3 1981 [3H]Pargyline was also bound to MAO A and B in a crude mitochondrial fraction from cultured skin fibroblasts of a male adult and to MAO B in blood platelets from the same individual. Tritium 1-3 monoamine oxidase A Homo sapiens 32-43 7264664-5 1981 For all tissues, SDS-PAGE of [3H]pargyline-bound samples revealed a labeled protein band of apparent molecular weight 63,000 for MAO A and 60,000 for MAO B. Tritium 30-32 monoamine oxidase A Homo sapiens 129-134 7264664-6 1981 When SDS-solubilized, [3H]pargyline-labeled MAO A and B proteins from the same male newborn were subjected to limited proteolysis and one-dimensional peptide mapping in SDS gels, different patterns of [3H]pargyline-labeled peptides were obtained. Tritium 23-25 monoamine oxidase A Homo sapiens 44-49 80324-7 1978 Finally, a correlation could be demonstrated between the frequency of PPD-reactive T cells and the [3H]dThd incorporation of 4 X 10(4) PPD-stimulated lymphocytes. Tritium 100-102 cellular communication network factor 6 Homo sapiens 70-73 80324-7 1978 Finally, a correlation could be demonstrated between the frequency of PPD-reactive T cells and the [3H]dThd incorporation of 4 X 10(4) PPD-stimulated lymphocytes. Tritium 100-102 cellular communication network factor 6 Homo sapiens 135-138 632283-3 1978 Of the 3H incorporated into individual apolipoproteins, separated by polyacrylamide gel electrophoresis, 25% was recovered in apolipoprotein B (apoB), 50% in apoA-I, 21% in apoA-IV, and 4% in C apolipoproteins, nearly all in apoC-II and apoC-III-0. Tritium 7-9 apolipoprotein A1 Rattus norvegicus 158-164 912599-1 1977 The stereochemical course of in vivo hydrolysis of triacylglycerols by lipoprotein lipase was investigated by determining the structure of diacylglycerol intermediates in postheparin plasma of rats which had been fed [3H]glycerol-labeled Intralipid 2 h before an injection of heparin or had been given an injection of a mixture of [3H]glycerol-Intralipid and heparin. Tritium 218-220 lipase G, endothelial type Rattus norvegicus 83-89 912599-1 1977 The stereochemical course of in vivo hydrolysis of triacylglycerols by lipoprotein lipase was investigated by determining the structure of diacylglycerol intermediates in postheparin plasma of rats which had been fed [3H]glycerol-labeled Intralipid 2 h before an injection of heparin or had been given an injection of a mixture of [3H]glycerol-Intralipid and heparin. Tritium 332-334 lipase G, endothelial type Rattus norvegicus 83-89 861949-5 1977 The addition of HPL increased the mean number of [3H]thymidine-radiolabeled epithelial cells (p less than 0.05) and the mean number of epithelial cells bearing mitotic figures (p less than 0.05). Tritium 50-52 galectin 1 Homo sapiens 16-19 7278870-0 1981 Isolation of hypoxanthine phosphoribosyltransferase-defective mutants in Chinese hamster V79 cells by tritium suicide. Tritium 102-109 hypoxanthine-guanine phosphoribosyltransferase Cricetulus griseus 13-51 7246069-3 1981 The immuno-precipitate caused by the reaction with anti-HPL serum in the crude extract from chorionic villi incubated with MEM medium containing [3H]-leucine was subjected to SDS gel electrophoresis. Tritium 146-148 galectin 1 Homo sapiens 56-59 8390115-2 1993 Angiotensin converting enzyme (ACE) activity was determined in confluent monolayers, under first-order reaction conditions, at 6, 24, 48, and 96 hr after treatment, using [3H]benzoyl-Phe-Ala-Pro as substrate. Tritium 172-174 angiotensin I converting enzyme Bos taurus 31-34 7227306-3 1981 Furthermore, [3H]mannose was incorporated into the 70,000 Mr peak, which corresponds to the parathyroid secretory protein (PSP) recognized as a glycoprotein. Tritium 14-16 phosphoserine phosphatase Bos taurus 92-121 861949-6 1977 [3H]Thymidine incorporation into chemically extracted DNA was also increased when HPL was added to the medium, although this increase did not quite achieve the 5% level of significance. Tritium 1-3 galectin 1 Homo sapiens 82-85 7227306-3 1981 Furthermore, [3H]mannose was incorporated into the 70,000 Mr peak, which corresponds to the parathyroid secretory protein (PSP) recognized as a glycoprotein. Tritium 14-16 phosphoserine phosphatase Bos taurus 123-126 8462464-4 1993 The results showed RLX (1-1000 ng/ml), insulin (1-1000 ng/ml), and IGF-I (1-30 ng/ml) alone stimulated [3H]thymidine incorporation into DNA by GC from small, medium, and large porcine follicles (P < 0.05). Tritium 104-106 prorelaxin Sus scrofa 19-22 8462464-4 1993 The results showed RLX (1-1000 ng/ml), insulin (1-1000 ng/ml), and IGF-I (1-30 ng/ml) alone stimulated [3H]thymidine incorporation into DNA by GC from small, medium, and large porcine follicles (P < 0.05). Tritium 104-106 insulin Sus scrofa 39-46 192733-11 1977 Whereas [3H]leucine incorporation into total protein is unaffected by estrogen treatment, [3H]leucine incorporation into cellular and secreted immunoprecipitable VLDL is markedly increased indicating specific activation of VLDL apopeptide synthesis; 8-10% of the labeled protein synthesized and secreted is VLDL. Tritium 91-93 very low density lipoprotein receptor Gallus gallus 162-166 192733-11 1977 Whereas [3H]leucine incorporation into total protein is unaffected by estrogen treatment, [3H]leucine incorporation into cellular and secreted immunoprecipitable VLDL is markedly increased indicating specific activation of VLDL apopeptide synthesis; 8-10% of the labeled protein synthesized and secreted is VLDL. Tritium 91-93 very low density lipoprotein receptor Gallus gallus 223-227 8462464-4 1993 The results showed RLX (1-1000 ng/ml), insulin (1-1000 ng/ml), and IGF-I (1-30 ng/ml) alone stimulated [3H]thymidine incorporation into DNA by GC from small, medium, and large porcine follicles (P < 0.05). Tritium 104-106 insulin like growth factor 1 Sus scrofa 67-72 192733-11 1977 Whereas [3H]leucine incorporation into total protein is unaffected by estrogen treatment, [3H]leucine incorporation into cellular and secreted immunoprecipitable VLDL is markedly increased indicating specific activation of VLDL apopeptide synthesis; 8-10% of the labeled protein synthesized and secreted is VLDL. Tritium 91-93 very low density lipoprotein receptor Gallus gallus 223-227 7220146-5 1981 To study this process, we administered 100 mg of tritiated bovine serum albumin ([3H]BSA) by gavage to rabbits at birth, one wk, 2 wk, 6 wk, and one year of age and measured plasma radioactivity 4 hr after gavage. Tritium 82-84 albumin Oryctolagus cuniculus 66-79 192733-12 1977 Dodecyl sulfate-acrylamide gel electrophoresis of immunoprecipitated 3H-VLDL reveals three major apopepetides of 300,000, 11,000, and 8,000 daltons corresponding to those of purified chicken VLDL. Tritium 69-71 very low density lipoprotein receptor Gallus gallus 72-76 7682222-1 1993 In the chemically transformed mouse fibroblasts (BP-A31) placed in a serum-free medium, the cdc2 mRNA content decreases in parallel with the cessation of [3H]thymidine incorporation. Tritium 155-157 cyclin-dependent kinase 1 Mus musculus 92-96 192305-8 1977 The C17 acids formed had a 3H/14C ratio similar to that of the C16 acids, indicating that most label incorporation into C17 was due to beta-oxidation to acetate followed by resynthesis into fatty acids. Tritium 27-29 cytokine like 1 Homo sapiens 4-7 7192625-4 1981 Localization on the autoradiograms of both [125I]RBP and [3H]retinol-RBP was interstitial; there was no interaction of RBP with cells in or on the seminiferous tubules. Tritium 58-60 retinol binding protein 4 Rattus norvegicus 69-72 7192625-4 1981 Localization on the autoradiograms of both [125I]RBP and [3H]retinol-RBP was interstitial; there was no interaction of RBP with cells in or on the seminiferous tubules. Tritium 58-60 retinol binding protein 4 Rattus norvegicus 69-72 8473484-2 1993 MBP inhibited specific binding of [3H]NMS to M2 receptors but not to M3 receptors. Tritium 35-37 myelin basic protein Homo sapiens 0-3 8473484-3 1993 MBP also inhibited atropine-induced dissociation of [3H]NMS-receptor complexes in a dose-dependent fashion, demonstrating that the interaction of MBP with the M2 muscarinic receptor is allosteric. Tritium 53-55 myelin basic protein Homo sapiens 0-3 6258708-3 1981 Whereas the selective delta-receptor agonist [3H]D-Ala2,D-Leu5-enkephalin specifically labelled binding sites in the CA2 area, [3H]etorphine grains displayed a uniform dense distribution throughout the pyramidal cell layers from CA1 to CA4. Tritium 46-48 proenkephalin Rattus norvegicus 63-73 867450-2 1977 It was shown that the steroid and amino acid were associated in a 1:2 or 1:3 ratio and evidence was provided by 3H-exchange for the interaction of tyrosine with ring A of estradiol at C-2 and C-4. Tritium 112-114 complement C2 Homo sapiens 184-187 8473484-3 1993 MBP also inhibited atropine-induced dissociation of [3H]NMS-receptor complexes in a dose-dependent fashion, demonstrating that the interaction of MBP with the M2 muscarinic receptor is allosteric. Tritium 53-55 myelin basic protein Homo sapiens 146-149 8473484-5 1993 Inhibition of [3H]NMS binding by MBP was reversible by treatment with heparin, which binds and neutralizes MBP. Tritium 15-17 myelin basic protein Homo sapiens 33-36 999825-3 1976 The zein mRNA sedimented as a homogeneous peak on sucrose gradients and contained a poly(A)-rich region based upon hybridization to [3H]poly(U). Tritium 133-135 zein Zea mays 4-8 10245-1 1976 The hydroxylation at C-2 of testosterone and 5alpha-dihydrotestosterone is measured by isolation of HTO after incubating "metabolically labile" tritium labeled steroids with the enzymes. Tritium 144-151 complement C2 Rattus norvegicus 21-24 6972416-3 1981 The resulting TCGF preparation is free of Con A as evidenced by its inability to activate fresh resting lymphoid cells and by measurements using [3H]Con A in tracer amounts. Tritium 146-148 interleukin 2 Mus musculus 14-18 7016116-5 1980 In the normal group, refeeding caused a biphasic increase in liver ornithine decarboxylase; there was a 20-fold increase in activity at 3h followed by a decrease in activity, and a second peak between 9 and 24h. Tritium 136-138 ornithine decarboxylase 1 Rattus norvegicus 67-90 7016116-7 1980 The increase in liver ornithine decarboxylase in diabetic rats was greater than in normal rats after 3h of refeeding, but there was no second peak. Tritium 101-103 ornithine decarboxylase 1 Rattus norvegicus 22-45 7016116-10 1980 Refeeding these rats after a 48h period without food caused a 5-fold increase in ornithine decarboxylase in skeletal muscle at 3h in control rats but failed to increase activity in diabetic rats. Tritium 127-129 ornithine decarboxylase 1 Rattus norvegicus 81-104 182538-0 1976 Interaction of 3H-labelled synthetic human gastrin I with rat gastric plasma membranes. Tritium 15-17 gastrin Homo sapiens 43-50 8473484-5 1993 Inhibition of [3H]NMS binding by MBP was reversible by treatment with heparin, which binds and neutralizes MBP. Tritium 15-17 myelin basic protein Homo sapiens 107-110 7689816-7 1993 When anti IGF-I antiserum was administrated to pregnant mice, both fetal and placental weight were significantly suppressed and transfer of 3H-AIB to fetus was also suppressed. Tritium 140-142 insulin-like growth factor 1 Mus musculus 10-15 1278160-2 1976 The effect of a number of different antibiotics and toxins on the capacity of Krebs II mouse ascites ribosomes to bind 3H-labelled elongation factors (EF-1 and EF-2) has been examined. Tritium 119-121 eukaryotic translation elongation factor 2 Mus musculus 151-164 6448924-2 1980 Definitive evidence for aromatase activity and oestrogen synthesis in preimplantation trophoblast was obtained for the pig with the formation of oestrone, oestradiol-17 beta and oestradiol-17 alpha from 3H-labelled androstenedione and dehydroepiandrosterone. Tritium 203-205 aromatase Bos taurus 24-33 7428109-6 1980 The induction of AHH by MC in these strains correlated well with the radioactive metabolites of [3H] BP remaining in the alkali extract derived from the AHH assay mixture and with the increased binding of [3H] BP to microsomal protein and DNA. Tritium 97-99 cytochrome P450, family 1, subfamily a, polypeptide 1 Mus musculus 17-20 7689816-8 1993 In contrast, antiserum to IGFBP-1 stimulated fetal growth and transfer of 3H-AIB to fetus. Tritium 74-76 insulin-like growth factor binding protein 1 Mus musculus 26-33 7428109-6 1980 The induction of AHH by MC in these strains correlated well with the radioactive metabolites of [3H] BP remaining in the alkali extract derived from the AHH assay mixture and with the increased binding of [3H] BP to microsomal protein and DNA. Tritium 206-208 cytochrome P450, family 1, subfamily a, polypeptide 1 Mus musculus 17-20 765113-10 1976 GnRH increased release of either [3H]leucine- or [3H]alanine-labeled LH into medium, but had no significant effect on incorporation of labeled amino acids into total LH in the system. Tritium 34-36 gonadotropin releasing hormone 1 Rattus norvegicus 0-4 8385528-0 1993 Chronic treatment with the monoamine oxidase inhibitors clorgyline and pargyline down-regulates non-adrenoceptor [3H]-idazoxan binding sites in the rat brain. Tritium 114-116 monoamine oxidase A Rattus norvegicus 27-44 8095334-6 1993 In situ hybridization of a 3H-labeled probe to human metaphase spreads localized the NDF gene to the short arm of chromosome 8 at bands p12-p21. Tritium 27-29 neuregulin 1 Homo sapiens 85-88 954783-5 1976 Basal and stimulated gastrin concentration in serum, as determined by radioimmunoassay, were higher 3 months after the operation and 3h after the test meal gastrin levels were still 50% higher than the basal levels. Tritium 133-135 gastrin Homo sapiens 156-163 6773577-4 1980 Binding for [125I]-alpha BuTx was slightly reduced by trypsin digestion of the membranes while both [125I]alpha BuTx and [3H]QNB binding were reduced by phospholipase A2 digestion (Crotalus adamantus phospholipase A2 and beta-bungarotoxin). Tritium 122-124 phospholipase A2 group IB Rattus norvegicus 153-169 8382518-6 1993 A single purified [3H]BZDC-IP3-labeled peptide, corresponding to IP3R amino acids 476-501, was sequenced and shown to match specific sequences in the N-terminal 20% of the IP3 receptor, an area suggested on the basis of mutagenesis studies to contain the IP3 recognition site. Tritium 19-21 inositol 1,4,5-trisphosphate receptor type 3 Homo sapiens 65-69 6261902-1 1980 The development profiles of [3H]methionine-enkephalin ([3H]Met-enk) binding sites and radioimmunoassayable (RIA) beta-endorphin in regions of rat brain were determined. Tritium 29-31 proenkephalin Rattus norvegicus 43-53 6261902-1 1980 The development profiles of [3H]methionine-enkephalin ([3H]Met-enk) binding sites and radioimmunoassayable (RIA) beta-endorphin in regions of rat brain were determined. Tritium 29-31 proenkephalin Rattus norvegicus 43-46 1249186-2 1976 Metabolism at the C-2 position was monitored by measuring the tritium incorporated into water in the incubate. Tritium 62-69 complement C2 Homo sapiens 18-21 8382518-6 1993 A single purified [3H]BZDC-IP3-labeled peptide, corresponding to IP3R amino acids 476-501, was sequenced and shown to match specific sequences in the N-terminal 20% of the IP3 receptor, an area suggested on the basis of mutagenesis studies to contain the IP3 recognition site. Tritium 19-21 inositol 1,4,5-trisphosphate receptor type 3 Homo sapiens 172-184 6261902-2 1980 The amount [3H]Met-enk bound reached its maximum in the 1st week after birth in the cerebellum, in the brainstem at the 2nd week, and in the whole forebrain at the 3rd week, that of RIA beta-endorphin reached its highest level at day 2 postpartum (p.p.) Tritium 12-14 proenkephalin Rattus norvegicus 19-22 6261902-7 1980 These findings suggest that both the development of RIA beta-endorphin and [3H]met-enk sites in rat brain follow a caudal to rostral sequence. Tritium 76-78 proenkephalin Rattus norvegicus 83-86 1165441-6 1975 Mesurement of [3H]thymidine uptake by isolated articular chondrocytes may provide a useful assay for both liver and serum somatomedin. Tritium 15-17 insulin-like growth factor 1 Rattus norvegicus 122-133 8440329-6 1993 IGF-I induced [3H]thymidine incorporation only weakly and only in the 2-h pulse. Tritium 15-17 insulin-like growth factor 1 Mus musculus 0-5 6261902-8 1980 Also, the close interrelationship between the elevation and decline in the amount of [3H]Met-enk bound and RIA beta-endorphin levels in each brain region suggests that these two components are important entities of the central nervous system. Tritium 86-88 proenkephalin Rattus norvegicus 93-96 4664584-2 1972 In a dimethyl sulphoxide-water medium it reacts rapidly (3h) with bovine insulin, and substitution occurs quantitatively at the N-terminal amino groups and at the in-amino groups of lysine residues. Tritium 57-59 insulin Bos taurus 73-80 8450176-3 1993 The purpose of these studies was to assess and characterize hypotensive responses to a 5-HT1A agonist, (8-hydroxy-dipropylaminotetraline, 8-OH-DPAT), administered to the ventral medulla of the rat, to correlate the responsive ventral medullary sites with the distribution of 3H-8-OH-DPAT binding sites, and to assess the role of serotonergic systems in mediating the hypotensive responses. Tritium 275-277 5-hydroxytryptamine receptor 1A Rattus norvegicus 87-93 4997465-0 1971 The preparation of tritium-labeled Fab antibody fragments. Tritium 19-26 FA complementation group B Homo sapiens 35-38 5685263-1 1968 When a 3-keto bile acid methyl ester was chromatographed on basic alumina inactivated with tritiated water, the enolic hydrogen atoms at C-2 and C-4 exchanged with tritium atoms. Tritium 164-171 complement C2 Homo sapiens 137-140 6256486-3 1980 UV irradiation caused an irreversible decrease in the total number of high-affinity [3H]STX binding sites in rat synaptosomes, while the dissociation constant of the remaining sites did not change. Tritium 85-87 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 Rattus norvegicus 88-91 6256486-6 1980 A22Na flux assay in irradiated synaptosomes directly demonstrated that [3H]STX binding sites and veratridine-stimulated, STX-blocked 22Na efflux had similar sensitivities to UV radiation. Tritium 72-74 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 Rattus norvegicus 75-78 6968757-1 1980 Antibodies to synthetic 3H-poly dAT have the advantage over antibody to native DNA in that they have a higher degree of specificity for systemic lupus erythematosus (SLE). Tritium 24-26 Dopamine transporter Drosophila melanogaster 32-35 6244551-1 1980 Stereospecific binding of 3H-labeled [D-Ala2,D-Leu5]enkephalin is irreversibly inactivated by the sulfhydryl group alkylating agent N-ethylmaleimide. Tritium 26-28 proenkephalin Rattus norvegicus 52-62 6244551-5 1980 [D-Ala2,Leu]Enkephalin and [D-Ala2,Met]enkephalin, however, are more effective (7 and 30 times, respectively) for the protection of 3H-labeled [D-Ala2,D-Leu5]enkephalin binding. Tritium 132-134 proenkephalin Rattus norvegicus 12-22 6244551-5 1980 [D-Ala2,Leu]Enkephalin and [D-Ala2,Met]enkephalin, however, are more effective (7 and 30 times, respectively) for the protection of 3H-labeled [D-Ala2,D-Leu5]enkephalin binding. Tritium 132-134 proenkephalin Rattus norvegicus 39-49 6244551-5 1980 [D-Ala2,Leu]Enkephalin and [D-Ala2,Met]enkephalin, however, are more effective (7 and 30 times, respectively) for the protection of 3H-labeled [D-Ala2,D-Leu5]enkephalin binding. Tritium 132-134 proenkephalin Rattus norvegicus 158-168 5685263-1 1968 When a 3-keto bile acid methyl ester was chromatographed on basic alumina inactivated with tritiated water, the enolic hydrogen atoms at C-2 and C-4 exchanged with tritium atoms. Tritium 164-171 complement C4A (Rodgers blood group) Homo sapiens 145-148 8419650-8 1993 Charge analysis of the [3H]galactose- and [3H]glucosamine-labeled glycopeptides from gp120 and gp160 indicates that approximately 14% of the complex-type N-linked oligosaccharides are sulfated. Tritium 24-26 glutamyl aminopeptidase Homo sapiens 95-100 8418891-8 1993 Efflux of [3H]cholesterol from plasma membranes was stimulated to similar extents by Lp A-I and Lp A-I/A-II isolated from either HDL2, HDL3 or plasma. Tritium 11-13 lipoprotein(a) Homo sapiens 85-89 5665127-0 1968 Stereochemistry of tritium at C-1 and C-7 in cholesterol derived from (3R,2R)-2T-mevalonic acid. Tritium 19-26 heterogeneous nuclear ribonucleoprotein C Homo sapiens 30-41 121266-1 1979 We report that after in vivo administration of (3H) tamoxifen, the cytosol and nuclear estrogen receptor sites of Rat uterus and Chicken oviduct are mostly occupied by polar metabolites. Tritium 47-50 estrogen receptor 1 Rattus norvegicus 87-104 8418891-8 1993 Efflux of [3H]cholesterol from plasma membranes was stimulated to similar extents by Lp A-I and Lp A-I/A-II isolated from either HDL2, HDL3 or plasma. Tritium 11-13 lipoprotein(a) Homo sapiens 96-100 497857-1 1979 The postnatal development of the estrogen receptor system of the rat brain is described by means of data from in vitro cytosol binding assays and from in vivo cell nuclear experiments using 3H-labeled estrogens. Tritium 190-192 estrogen receptor 1 Rattus norvegicus 33-50 8418891-8 1993 Efflux of [3H]cholesterol from plasma membranes was stimulated to similar extents by Lp A-I and Lp A-I/A-II isolated from either HDL2, HDL3 or plasma. Tritium 11-13 junctophilin 3 Homo sapiens 129-133 8495229-2 1993 At concentrations above 1.0 ng/ml, both native and recombinant human HGF/SF stimulated the DNA synthesis determined by [3H]thymidine incorporation, which was completely inhibited by an anti-human HGF/SF monoclonal antibody. Tritium 120-122 hepatocyte growth factor Homo sapiens 69-75 531226-2 1979 Binding assays revealed the existence of high affinity binding of 3H-prostaglandin E2 (3H-PGE2) and 3H-prostaglandin F1 alpha (3H-PGF2 alpha) in low speed supernatants of pineal homogenates. Tritium 66-68 prostaglandin F synthase 2 Bos taurus 130-134 33935047-4 2021 The uptake of [3H]L-lysine was Na+-independent, voltage-sensitive, and strongly inhibited by inhibitors and substrates of cationic amino acid transporter 1 (system y+). Tritium 15-17 solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 Mus musculus 122-155 531226-6 1979 A single population of binding sites for 3H-PGF2 alpha was also detected with a Kd= 1.7 nM and a similar binding site concentration. Tritium 41-43 prostaglandin F synthase 2 Bos taurus 44-48 8495229-2 1993 At concentrations above 1.0 ng/ml, both native and recombinant human HGF/SF stimulated the DNA synthesis determined by [3H]thymidine incorporation, which was completely inhibited by an anti-human HGF/SF monoclonal antibody. Tritium 120-122 hepatocyte growth factor Homo sapiens 196-202 531226-8 1979 Unlabeled PGF1 was relatively ineffective to compete for 3H-PGE2 binding (ED50 greater than 1000 nM) but displaced effectively 3H-PGF2 alpha binding (ED20=1.2 nM). Tritium 127-129 prostaglandin F synthase 2 Bos taurus 10-14 531226-8 1979 Unlabeled PGF1 was relatively ineffective to compete for 3H-PGE2 binding (ED50 greater than 1000 nM) but displaced effectively 3H-PGF2 alpha binding (ED20=1.2 nM). Tritium 127-129 prostaglandin F synthase 2 Bos taurus 130-134 32970217-13 2021 CONCLUSIONS: 3H-MK6240 has a high binding affinity for tau deposits in AD brain tissue but also has different binding characteristics from those of the first-generation tau tracers. Tritium 13-15 microtubule associated protein tau Homo sapiens 55-58 8093604-1 1993 We examined the effect of three distinct depolarizing conditions on [3H]ACh release from cardiac postganglionic parasympathetic neurons in age-matched controls and insulin-treated STZ-induced diabetic rats to determine whether alterations in neurotransmitter release were present in the diabetic group. Tritium 69-71 acyl-CoA thioesterase 12 Rattus norvegicus 72-75 32970217-13 2021 CONCLUSIONS: 3H-MK6240 has a high binding affinity for tau deposits in AD brain tissue but also has different binding characteristics from those of the first-generation tau tracers. Tritium 13-15 microtubule associated protein tau Homo sapiens 169-172 509696-2 1979 Measurements on the radioactivity of nuclear components after in vivo treatment with [3H]DBD for 1 h indicated preferential drug binding to the high molecular weight component of the nuclear residual acidic protein (non-histones) and also to Histone 1 (H1) (very lysine rich, F1). Tritium 86-88 H1.5 linker histone, cluster member Homo sapiens 242-251 509696-2 1979 Measurements on the radioactivity of nuclear components after in vivo treatment with [3H]DBD for 1 h indicated preferential drug binding to the high molecular weight component of the nuclear residual acidic protein (non-histones) and also to Histone 1 (H1) (very lysine rich, F1). Tritium 86-88 H1.5 linker histone, cluster member Homo sapiens 253-255 33248188-6 2021 Our results indicate that chronic developmental Pb2+ exposure increases the levels of [3H]-DAMGO specific binding to MOR in juvenile and early adolescent Pb2+-exposed male and female rat brain with no changes in late-adolescent (PN50) and minor changes in Pb2+-exposed adult male rats (PN120). Tritium 87-89 opioid receptor mu 1 Homo sapiens 117-120 33222700-8 2020 3H-MK6240 Tau binding correlated with AT8 immunostaining but to a lesser degree than the 3H-THK5117 tracer, suggesting differences in binding sites between the two Tau tracers. Tritium 0-2 microtubule associated protein tau Homo sapiens 10-13 33222700-8 2020 3H-MK6240 Tau binding correlated with AT8 immunostaining but to a lesser degree than the 3H-THK5117 tracer, suggesting differences in binding sites between the two Tau tracers. Tritium 0-2 microtubule associated protein tau Homo sapiens 164-167 383231-2 1979 These cells actively incorporated [3H]leucine into proinsulin and insulin. Tritium 35-37 insulin Bos taurus 51-61 383231-2 1979 These cells actively incorporated [3H]leucine into proinsulin and insulin. Tritium 35-37 insulin Bos taurus 54-61 87244-4 1979 6-Hydroxydopamine variably blocked transport of all 4 waves of 3H protein and bilateral injections decreased hypothalamic levels of TH, DBH and NE by 58.2, 56.9 and 52.2% of controls, respectively. Tritium 63-65 dopamine beta-hydroxylase Rattus norvegicus 136-139 92923-4 1979 3.--After intracisternally injection of [14C]DA or [3H]NA, SAH increases the level of [14C]MD and [3H]NM. Tritium 99-101 acyl-CoA synthetase medium-chain family member 3 Rattus norvegicus 59-62 8093604-3 1993 We also studied the effect of STZ-induced diabetes on [3H]ACh release by the relatively site-specific depolarizing agent VT (preferentially acts at the axon) and high potassium (non-site-specific). Tritium 55-57 acyl-CoA thioesterase 12 Rattus norvegicus 58-61 8093604-4 1993 Basal, SRIF14-(10(-7) M), VT-(10(-4) M), and K+ (100 mM)-stimulated [3H]ACh release was similar in control and STZ-induced diabetic animals. Tritium 69-71 acyl-CoA thioesterase 12 Rattus norvegicus 72-75 31540330-4 2019 The mGluR1/5 agonist (S)-3,5-Dihydroxyphenylglycine (3,5-DHPG) concentration dependently stimulated the release of [3H]d-Aspartate ([3H]d-Asp), which was comparable in 30- and 60-day-old wild type mice and SOD1G93A mice. Tritium 116-118 glutamate receptor, metabotropic 1 Mus musculus 4-10 31540330-4 2019 The mGluR1/5 agonist (S)-3,5-Dihydroxyphenylglycine (3,5-DHPG) concentration dependently stimulated the release of [3H]d-Aspartate ([3H]d-Asp), which was comparable in 30- and 60-day-old wild type mice and SOD1G93A mice. Tritium 133-135 glutamate receptor, metabotropic 1 Mus musculus 4-10 8093604-5 1993 However, in STZ-induced diabetic but not control rats, SRIF14-induced [3H]ACh release was resistant to TTX (2 x 10(-7) M). Tritium 71-73 acyl-CoA thioesterase 12 Rattus norvegicus 74-77 460548-0 1979 The membrane binding of choline acetyltransferase: uptake and acetylation of [3H]-choline in rabbit cortical slices. Tritium 78-80 LOW QUALITY PROTEIN: choline O-acetyltransferase Oryctolagus cuniculus 24-49 8380386-14 1993 DBI added to MA-10 cells affected DNA synthesis and cell growth in a biphasic manner; at low concentrations (1 nM), DBI was mitogenic, increasing [3H]thymidine incorporation and cell numbers by 30-40%, while at high concentrations (1 microM), DBI inhibited cell growth (30-40%). Tritium 147-149 diazepam binding inhibitor Mus musculus 0-3 481582-3 1979 DES-associated radioactivity in the fetal plasma approximated maternal plasma one-half hour after iv administration of [3H]DES; [3H]-acitivity associated with DES in the fetal genital tract was about threefold higher. Tritium 120-122 desmin Mus musculus 0-3 481582-3 1979 DES-associated radioactivity in the fetal plasma approximated maternal plasma one-half hour after iv administration of [3H]DES; [3H]-acitivity associated with DES in the fetal genital tract was about threefold higher. Tritium 120-122 desmin Mus musculus 123-126 8380386-14 1993 DBI added to MA-10 cells affected DNA synthesis and cell growth in a biphasic manner; at low concentrations (1 nM), DBI was mitogenic, increasing [3H]thymidine incorporation and cell numbers by 30-40%, while at high concentrations (1 microM), DBI inhibited cell growth (30-40%). Tritium 147-149 diazepam binding inhibitor Mus musculus 116-119 30630742-9 2019 Hepcidin-25 concentrations increased 3h post-exercise (p<0.001), however, they did not differ between trials (p=0.46) or diets (p=0.84). Tritium 37-39 hepcidin antimicrobial peptide Homo sapiens 0-8 8380386-14 1993 DBI added to MA-10 cells affected DNA synthesis and cell growth in a biphasic manner; at low concentrations (1 nM), DBI was mitogenic, increasing [3H]thymidine incorporation and cell numbers by 30-40%, while at high concentrations (1 microM), DBI inhibited cell growth (30-40%). Tritium 147-149 diazepam binding inhibitor Mus musculus 116-119 457625-8 1979 The sucrose density gradient centrifugation patterns of 131I and 3H-radioactivity of cytoplasmic extracts indicated that 131I-thyroglobulin is contained in particulates, especially in vesicles with low density(d=1.12) and that some of them are released into the soluble fraction within 10 min. Tritium 65-67 thyroglobulin Sus scrofa 126-139 8383782-4 1993 In cultured rabbit proximal tubule cells, recombinant human HGF (10(-10) M) increased DNA synthesis, measured as [3H] thymidine incorporation, from 1345 +/- 213 to 2931 +/- 636 cpm/10(6) cells; n = 9; p < 0.005). Tritium 114-116 hepatocyte growth factor Homo sapiens 60-63 17734139-2 1979 The tritium results show that the waters below the ice have exchanged with Ross Sea water since the advent of nuclear testing. Tritium 4-11 S13 erythroblastosis (avian) oncogene homolog Homo sapiens 80-83 31019305-4 2019 Comparison of the structures of MT2 with a published structure8 of MT1 reveals that, despite conservation of the orthosteric ligand-binding site residues, there are notable conformational variations as well as differences in [3H]melatonin dissociation kinetics that provide insights into the selectivity between melatonin receptor subtypes. Tritium 226-228 metallothionein 2A Homo sapiens 32-35 31019305-4 2019 Comparison of the structures of MT2 with a published structure8 of MT1 reveals that, despite conservation of the orthosteric ligand-binding site residues, there are notable conformational variations as well as differences in [3H]melatonin dissociation kinetics that provide insights into the selectivity between melatonin receptor subtypes. Tritium 226-228 metallothionein 1I, pseudogene Homo sapiens 67-70 8383782-6 1993 HGF was additive with EGF in stimulating [3H] thymidine incorporation. Tritium 42-44 pro-epidermal growth factor Oryctolagus cuniculus 22-25 8429920-10 1993 The efficacy of lovastatin blockage of protein methylation and enhancement of 3H-MVA-metabolites incorporation into GTP-binding proteins was lower in NGF-induced cells than in controls. Tritium 78-80 nerve growth factor Rattus norvegicus 150-153 1429634-2 1992 In attempting to isolate and biochemically characterize mammalian proteins capable of regulating various activities of CDC42Hs, we have identified an activity in bovine brain cytosol which effectively inhibits the dissociation of [3H]GDP from the platelet- or the Spodoptera frugiperda-expressed CDC42Hs protein. Tritium 231-233 cell division cycle 42 Homo sapiens 119-126 30900874-7 2019 Conversely, filter binding assays demonstrated that EFV inhibited 3H-labeled dNTP binding to RT-P/T complexes with displacement of Mn2+-dNTP complexes requiring much greater concentrations of EFV than the more weakly bound Mg2+-dNTP complexes. Tritium 66-68 MORN repeat containing 4 Homo sapiens 93-99 155649-1 1979 We detected lipase activity in an homogenate of mouse spleen- and lymph node cells by using [3H]-triolein as a lipase substrate. Tritium 93-95 lipase, endothelial Mus musculus 12-18 155649-1 1979 We detected lipase activity in an homogenate of mouse spleen- and lymph node cells by using [3H]-triolein as a lipase substrate. Tritium 93-95 lipase, endothelial Mus musculus 111-117 160192-2 1979 Together with the post-mortem stability exhibited for [3H]GABA-binding, this provides an approach for determining the functional state of the GABA-receptor in various disease states; (2) In Parkinson"s disease [3H]GABA-binding in the substantia nigra is significantly decreased whereas that in the putamen and caudate nucleus is unaltered. Tritium 55-57 GABA type A receptor-associated protein Homo sapiens 142-155 160192-2 1979 Together with the post-mortem stability exhibited for [3H]GABA-binding, this provides an approach for determining the functional state of the GABA-receptor in various disease states; (2) In Parkinson"s disease [3H]GABA-binding in the substantia nigra is significantly decreased whereas that in the putamen and caudate nucleus is unaltered. Tritium 211-213 GABA type A receptor-associated protein Homo sapiens 142-155 363726-5 1978 Synthesis and secretion of fibronectin into the medium are shown by anabolic labeling with [35S]methionine or [3H]glycine, and identification of the secreted proteins by immunoprecipitation and sodium dodecyl sulfate (SDS)-disc gel electrophoresis. Tritium 111-113 fibronectin 1 Gallus gallus 27-38 30959853-2 2019 The BHC/AgNW/THC TCFs show high scratch resistance with a hardness of 3H due to the enhanced adhesion to the substrate. Tritium 70-72 WASP actin nucleation promoting factor Homo sapiens 13-16 30359033-6 2018 The protein expression and activity of OATP2B1 were also decreased by APNP exposure, as determined by Western blotting and measurements of [3H]estrone-3-sulfate uptake by Caco-2 cells, respectively. Tritium 140-142 solute carrier organic anion transporter family member 2B1 Homo sapiens 39-46 1429634-2 1992 In attempting to isolate and biochemically characterize mammalian proteins capable of regulating various activities of CDC42Hs, we have identified an activity in bovine brain cytosol which effectively inhibits the dissociation of [3H]GDP from the platelet- or the Spodoptera frugiperda-expressed CDC42Hs protein. Tritium 231-233 cell division cycle 42 Homo sapiens 296-303 29185365-3 2018 Among them, compound 3f (IC50 = 1.05 +- 0.17 microM) and 3h (IC50 = 0.96 +- 0.18 microM) exhibited potent inhibition of PKM2, and their inhibitory activities are superior to compound 3k (IC50 = 2.95 +- 0.53 microM) and the known PKM2 inhibitor shikonin (IC50 = 8.82 +- 2.62 microM). Tritium 57-59 pyruvate kinase M1/2 Homo sapiens 120-124 29185365-3 2018 Among them, compound 3f (IC50 = 1.05 +- 0.17 microM) and 3h (IC50 = 0.96 +- 0.18 microM) exhibited potent inhibition of PKM2, and their inhibitory activities are superior to compound 3k (IC50 = 2.95 +- 0.53 microM) and the known PKM2 inhibitor shikonin (IC50 = 8.82 +- 2.62 microM). Tritium 57-59 pyruvate kinase M1/2 Homo sapiens 229-233 1328234-4 1992 These three types of cell lines were cultured in the presence of 50 nM [3H]retinoic acid, and the metabolism of retinoic acid was followed over the next 24 h. The results demonstrate that CRABP-I has the ability to alter both the levels and types of RA metabolites produced in the cytoplasm of differentiating embryonic stem cells. Tritium 72-75 cellular retinoic acid binding protein 1 Homo sapiens 188-195 30279647-6 2018 The ( +- )baclofen-induced inhibition of [3H]GABA exocytosis was more pronounced in cortical synaptosomes from Grm1crv4/crv4 mice, which bear a spontaneous mutation of the Grm1 gene leading to the functional inactivation of the mGlu1 receptor. Tritium 42-44 glutamate receptor, metabotropic 1 Mus musculus 111-115 215286-5 1978 Treatment of the purified material with different detergents resulted in loss of all [3H]oxytocin binding capacity. Tritium 86-88 oxytocin Oryctolagus cuniculus 89-97 1444891-5 1992 Inhibitory effects of insulin on the [3H]-thymidine incorporation were potentiated by EGF, though EGF alone strongly increased the effect; whereas the addition of TGF-beta had no significant effect on the insulin action. Tritium 38-40 epidermal growth factor like 1 Rattus norvegicus 86-89 714185-3 1978 The 14C-oxalate to 3H-inulin clearance ratio (Cox/Cin) was 1.36 +/- 0.04 and was lowered by probenecid (200 mg/kg) to 1.11 +/- 0.03 (+/- S.E., n = 6, P less than 0.005). Tritium 19-21 coproporphyrinogen oxidase Rattus norvegicus 46-49 691975-2 1978 Phlorizin also diminishes the rate of 3H-PAH washout from preloaded slices into PAH-free medium. Tritium 38-40 phenylalanine hydroxylase Rattus norvegicus 41-44 29748333-6 2018 In parallel, plasma 3H-cholesterol counts from the EL-expressing mice decreased by 85% compared with control. Tritium 20-22 lipase, endothelial Mus musculus 51-53 29636415-5 2018 Here, using [3H]phorbol 12,13-dibutyrate ([3H]PDBu) competition binding assays, we found that a synthetic DAG-lactone, AJH-836, preferentially binds to the novel PKC isoforms PKCdelta and PKCepsilon relative to classical PKCalpha and PKCbetaII. Tritium 13-15 protein kinase C epsilon Homo sapiens 188-198 691975-2 1978 Phlorizin also diminishes the rate of 3H-PAH washout from preloaded slices into PAH-free medium. Tritium 38-40 phenylalanine hydroxylase Rattus norvegicus 80-83 1468448-7 1992 3T3 [3H]thymidine incorporation assays indicated that the beta-D xyloside-induced reduction of matrix-associated bFGF coincided with a significant increase in bFGF activity in the conditioned media. Tritium 5-7 fibroblast growth factor 2 Mus musculus 113-117 691975-3 1978 At higher concentrations, phlorizin (5.0 mM) reduces slice uptake of 3H-PAH following short incubations but increases 3H-PAH accumulation after more prolonged incubations. Tritium 69-71 phenylalanine hydroxylase Rattus norvegicus 72-75 713129-3 1978 HPL responded will to PHA while n-ML did not, as assessed by 3H-TdR incorporation in the presence of the mitogen. Tritium 61-63 galectin 1 Homo sapiens 0-3 28970331-3 2018 Methods: RO6958948, RO6931643, and RO6924963 were identified as high-affinity competitors at the 3H-T808 binding site on native tau aggregates in human late-stage AD brain tissue. Tritium 97-99 microtubule associated protein tau Homo sapiens 128-131 1358662-2 1992 Since [3H]emonapride ([3H]YM-09151-2), a benzamide neuroleptic, consistently detects more dopamine D2 receptors than [3H]spiperone in the same tissue, we tested whether this property was inherent in the cloned dopamine D2 receptor. Tritium 23-25 dopamine receptor D2 Homo sapiens 90-110 28970331-6 2018 Results:3H-RO6958948, 3H-RO6931643, and 3H-RO6924963 bound with high affinity and specificity to tau aggregates, clearly lacking affinity for concomitant Abeta plaques in human AD Braak V tissue sections. Tritium 8-10 microtubule associated protein tau Homo sapiens 97-100 28970331-7 2018 The specificity of all 3 radioligands for tau aggregates was supported, first, by binding patterns in AD sections comparable to the tau-specific radioligand 3H-T808; second, by very low nonspecific binding in brain tissue devoid of tau pathology, excluding significant radioligand binding to any other central nervous system target; and third, by macroscopic and microscopic colocalization and quantitative correlation of radioligand binding and tau antibody staining on the same tissue section. Tritium 157-159 microtubule associated protein tau Homo sapiens 42-45 28970331-7 2018 The specificity of all 3 radioligands for tau aggregates was supported, first, by binding patterns in AD sections comparable to the tau-specific radioligand 3H-T808; second, by very low nonspecific binding in brain tissue devoid of tau pathology, excluding significant radioligand binding to any other central nervous system target; and third, by macroscopic and microscopic colocalization and quantitative correlation of radioligand binding and tau antibody staining on the same tissue section. Tritium 157-159 microtubule associated protein tau Homo sapiens 132-135 28970331-7 2018 The specificity of all 3 radioligands for tau aggregates was supported, first, by binding patterns in AD sections comparable to the tau-specific radioligand 3H-T808; second, by very low nonspecific binding in brain tissue devoid of tau pathology, excluding significant radioligand binding to any other central nervous system target; and third, by macroscopic and microscopic colocalization and quantitative correlation of radioligand binding and tau antibody staining on the same tissue section. Tritium 157-159 microtubule associated protein tau Homo sapiens 132-135 28970331-7 2018 The specificity of all 3 radioligands for tau aggregates was supported, first, by binding patterns in AD sections comparable to the tau-specific radioligand 3H-T808; second, by very low nonspecific binding in brain tissue devoid of tau pathology, excluding significant radioligand binding to any other central nervous system target; and third, by macroscopic and microscopic colocalization and quantitative correlation of radioligand binding and tau antibody staining on the same tissue section. Tritium 157-159 microtubule associated protein tau Homo sapiens 132-135 29364035-7 2018 Consistent with increased [3H]WIN 35428 binding, dopamine transporter-specific [3H]dopamine uptake in RGS12-null ventral striatal synaptosomes was found to be increased. Tritium 80-82 regulator of G-protein signaling 12 Mus musculus 102-107 618728-6 1978 Thirdly, naloxone, morphine, levorphanol and its non-analgesic enantiomer dextrorphan displaced 3H-GABA from GABA receptor sites in homogenates of human cerebellum, all with comparable low potencies (IC50 = 250--400 micron). Tritium 96-98 GABA type A receptor-associated protein Homo sapiens 109-122 627404-1 1978 The hydroxylation of testosterone and 5alpha-dihydrotestosterone at C-2 was measured quantitatively in vivo and in vitro in rats by using "metabolically labile" tritium labelled substrates. Tritium 161-168 complement C2 Rattus norvegicus 68-71 894168-0 1977 An assay for plasma somatomedin: [3H]thymidine incorporation by isolated rabbit chondrocytes. Tritium 34-36 insulin-like growth factor I Oryctolagus cuniculus 20-31 1358662-2 1992 Since [3H]emonapride ([3H]YM-09151-2), a benzamide neuroleptic, consistently detects more dopamine D2 receptors than [3H]spiperone in the same tissue, we tested whether this property was inherent in the cloned dopamine D2 receptor. Tritium 23-25 dopamine receptor D2 Homo sapiens 90-110 1358662-5 1992 The apparently different dopamine D2 receptor densities revealed by these two types of 3H-ligands (i.e. [3H]spiperone and the [3H]benzamides), therefore, arise from an inherent property of the dopamine D2 receptor protein. Tritium 87-89 dopamine receptor D2 Homo sapiens 25-45 562863-3 1977 This paper deals with the effect in vitro of flavonoids and flavonoid-copper complexes on the oxidative deamination of lysine epsilon-amino groups in [4,5-3H]-lysine-labelled elastin. Tritium 155-157 elastin Mus musculus 175-182 1358662-5 1992 The apparently different dopamine D2 receptor densities revealed by these two types of 3H-ligands (i.e. [3H]spiperone and the [3H]benzamides), therefore, arise from an inherent property of the dopamine D2 receptor protein. Tritium 87-89 dopamine receptor D2 Homo sapiens 193-213 29414147-7 2018 Clobenpropit inhibited [3H]-dopamine uptake (maximum inhibition 82.7+-2.8%, IC50 490nM), and the effect was reproduced by the H3R antagonist/inverse agonist iodophenpropit, but not by the agonists R-alpha-methylhistamine and immepip or the antagonists/inverse agonists ciproxifan and A-331440. Tritium 24-26 histamine receptor H3 Homo sapiens 126-129 1358662-5 1992 The apparently different dopamine D2 receptor densities revealed by these two types of 3H-ligands (i.e. [3H]spiperone and the [3H]benzamides), therefore, arise from an inherent property of the dopamine D2 receptor protein. Tritium 105-107 dopamine receptor D2 Homo sapiens 25-45 1358662-5 1992 The apparently different dopamine D2 receptor densities revealed by these two types of 3H-ligands (i.e. [3H]spiperone and the [3H]benzamides), therefore, arise from an inherent property of the dopamine D2 receptor protein. Tritium 105-107 dopamine receptor D2 Homo sapiens 193-213 1452414-4 1992 Parameters of anti-CD3-induced T-cell activation included 3H-TdR incorporation (+/- recombinant murine IL-2), and flow cytometric analysis of CD3 and IL-2 receptor (IL-2R) expression on CD4+ and CD8+ cells. Tritium 58-60 CD3 antigen, epsilon polypeptide Mus musculus 19-22 321219-5 1977 Both EB and GnRH significantly elevated the amount of [3H]glucosamine-LH appearing in the medium, the tissue, and the total system (medium + tissue), but they increased the amount of [14C]alanine-LH only in the medium. Tritium 55-58 gonadotropin releasing hormone 1 Rattus norvegicus 12-16 1452414-6 1992 Increased 3H-TdR incorporation was maximal over a dose range of 0.25-25 micrograms anti-CD3, while maximal increases in the percentage of CD4+ and CD8+ cycling occurred after a dose of 2.5 micrograms anti-CD3. Tritium 10-12 CD3 antigen, epsilon polypeptide Mus musculus 88-91 192733-12 1977 Dodecyl sulfate-acrylamide gel electrophoresis of immunoprecipitated 3H-VLDL reveals three major apopepetides of 300,000, 11,000, and 8,000 daltons corresponding to those of purified chicken VLDL. Tritium 69-71 very low density lipoprotein receptor Gallus gallus 191-195 29248751-4 2018 The compounds 3f and 3h showed promising hMAO-A inhibition with an IC50 values of 0.012 muM and 0.011 muM and significant selectivity indexes of 1214 and 1601 towards hMAO-A, respectively. Tritium 21-23 monoamine oxidase A Homo sapiens 41-47 29248751-4 2018 The compounds 3f and 3h showed promising hMAO-A inhibition with an IC50 values of 0.012 muM and 0.011 muM and significant selectivity indexes of 1214 and 1601 towards hMAO-A, respectively. Tritium 21-23 monoamine oxidase A Homo sapiens 167-173 1358403-1 1992 The binding profile of [3H]8-hydroxy-2-(di-N-propylamino)-tetralin ([3H]8-OH-DPAT) to serotonin1A (5-HT1A) sites in rat hippocampal, frontocortical and striatal membranes has been compared. Tritium 24-27 5-hydroxytryptamine receptor 1A Rattus norvegicus 99-105 29248751-5 2018 The mechanism of hMAO-A inhibition of compounds 3f and 3h was investigated by Lineweaver-Burk graphics and reversible-competitive inhibition of hMAO-A was determined. Tritium 55-57 monoamine oxidase A Homo sapiens 17-23 29248751-5 2018 The mechanism of hMAO-A inhibition of compounds 3f and 3h was investigated by Lineweaver-Burk graphics and reversible-competitive inhibition of hMAO-A was determined. Tritium 55-57 monoamine oxidase A Homo sapiens 144-150 1380434-6 1992 In particular, addition of increasing concentrations of EGF from 0.25-10 ng/ml to a constant concentration of 50 ng/ml IGF-I resulted in significant potentiation of [3H]thymidine incorporation of astroglial cells. Tritium 166-168 epidermal growth factor like 1 Rattus norvegicus 56-59 29248751-8 2018 The docking study of compound 3f and 3h revealed that there is a strong interaction between the active sites of hMAO-A and analyzed compound. Tritium 37-39 monoamine oxidase A Homo sapiens 112-118 17756096-0 1977 Tritium-helium dating in the sargasso sea: a measurement of oxygen utilization rates. Tritium 0-7 S13 erythroblastosis (avian) oncogene homolog Homo sapiens 38-41 1510725-11 1992 Photoaffinity labeling of TPMT with [3H]Ado-Met should make it possible to purify the enzyme to homogeneity and to study amino acid sequences at or near its active site. Tritium 37-39 thiopurine S-methyltransferase Homo sapiens 26-30 191181-4 1977 Newly synthesized 3H-casein and 3H-alphaLA were separately precipitated with specific antibodies and identified by their mobilities on sodium dodecyl sulfate-acrylamide gels and by competition with nonradioactive casein and alphaLA. Tritium 32-34 lactalbumin, alpha Rattus norvegicus 35-42 193169-4 1977 However, opiates without the oxygen bridge as in the structure of morphine, such as levorphanol and the benzomorphans show affinities for the receptors of 3H-enkephalin equal or greater than their affinities for the receptors of 3H-opiates. Tritium 155-157 proenkephalin Rattus norvegicus 158-168 138443-8 1977 Treatment of the enzyme with phospholipase A results in time-dependent changes in the number of binding sites for [3H]ouabain at equilibrium. Tritium 115-117 phospholipase A and acyltransferase 1 Homo sapiens 29-44 138443-10 1977 Treatment of the membrane enzyme preparations with detergents reveals additional [3H]ouabain binding sites which are extremely sensitive to lipolysis with phospholipase A. Tritium 82-84 phospholipase A and acyltransferase 1 Homo sapiens 155-170 28796418-6 2017 By photolabeling experiments performed with a nitrophenylazide containing GM1 oligosaccharide, labeled with tritium, we showed a direct interaction between the GM1 oligosaccharide and the extracellular domain of TrkA receptor. Tritium 108-115 neurotrophic tyrosine kinase, receptor, type 1 Mus musculus 212-216 28789975-4 2017 NPY injection increased glycerol-3-phosphate dehydrogenase (G3PDH) activity at 1 and 3h and reduced plasma non-esterified fatty acids (NEFAs) at 1 and 12h. Tritium 85-87 neuropeptide Y Homo sapiens 0-3 28630284-5 2017 With OAT3, a dose-dependent inhibition of [3H]ES uptake and a downward shift in [3H]ES equilibrium were observed, indicating that all four drugs bind to OAT3 and may possibly be translocated. Tritium 43-45 solute carrier family 22 member 8 Homo sapiens 5-9 28630284-5 2017 With OAT3, a dose-dependent inhibition of [3H]ES uptake and a downward shift in [3H]ES equilibrium were observed, indicating that all four drugs bind to OAT3 and may possibly be translocated. Tritium 81-83 solute carrier family 22 member 8 Homo sapiens 5-9 28630284-5 2017 With OAT3, a dose-dependent inhibition of [3H]ES uptake and a downward shift in [3H]ES equilibrium were observed, indicating that all four drugs bind to OAT3 and may possibly be translocated. Tritium 81-83 solute carrier family 22 member 8 Homo sapiens 153-157 28630284-8 2017 Using [3H]PAH as a substrate of OAT1, nalidixic acid inhibited but dantrolene, glafenine, and prazosin stimulated uptake. Tritium 7-9 solute carrier family 22 member 6 Homo sapiens 32-36 28034785-11 2017 The results indicated that in the CA1 region of the hippocampus the 5-HT5A, 5-HT6 and 5-HT7 serotonin receptors participate in the reconsolidation of the CFC memory 3h post-reactivation. Tritium 165-167 carbonic anhydrase 1 Rattus norvegicus 34-37 194233-4 1977 After reaction of 3H-peptide with cytochrome c, about one-third of the tritium counts migrated with cytochrome c during gel filtration. Tritium 18-20 cytochrome c, somatic Equus caballus 34-46 194233-4 1977 After reaction of 3H-peptide with cytochrome c, about one-third of the tritium counts migrated with cytochrome c during gel filtration. Tritium 18-20 cytochrome c, somatic Equus caballus 100-112 1356570-13 1992 In the rat spinal cord [3H]-MK 912 bound to alpha 2A- and alpha 2C-adrenoceptor sites with similar affinities as in the cerebral cortex and with densities 172 and 7.4 fmol mg-1 protein, respectively. Tritium 24-26 adrenoceptor alpha 2A Rattus norvegicus 44-79 194233-4 1977 After reaction of 3H-peptide with cytochrome c, about one-third of the tritium counts migrated with cytochrome c during gel filtration. Tritium 71-78 cytochrome c, somatic Equus caballus 34-46 194233-4 1977 After reaction of 3H-peptide with cytochrome c, about one-third of the tritium counts migrated with cytochrome c during gel filtration. Tritium 71-78 cytochrome c, somatic Equus caballus 100-112 28669381-11 2017 Bovine MPO may have a lethal effect on T. gondii tachyzoites in vitro during a 3h incubation. Tritium 79-81 myeloperoxidase Bos taurus 7-10 1421756-3 1992 A 70 kDa fragment from the external domain of the TfR was generated by trypsin treatment of the [3H]glucosamine-labelled receptor purified from human K562 cells. Tritium 97-99 transferrin receptor Homo sapiens 50-53 28138029-7 2017 Two FRalpha knockdown clones (KD-4 and KD-10) showed markedly reduced binding and uptake of [3H]folic acid and [3H]AGF154 by FRalpha, but maintained high levels of [3H]AGF154 uptake by PCFT compared to nontargeted control cells. Tritium 93-95 FOS like 1, AP-1 transcription factor subunit Homo sapiens 4-11 28138029-7 2017 Two FRalpha knockdown clones (KD-4 and KD-10) showed markedly reduced binding and uptake of [3H]folic acid and [3H]AGF154 by FRalpha, but maintained high levels of [3H]AGF154 uptake by PCFT compared to nontargeted control cells. Tritium 112-114 FOS like 1, AP-1 transcription factor subunit Homo sapiens 4-11 824287-6 1976 The uptake of [3H]retinol by the cells could be inhibited by RBP containing unlabeled retinol, vitamin A-depleted RBP, and Fab" fragments against RBP. Tritium 15-17 FA complementation group B Homo sapiens 123-126 1504012-11 1992 (4) Comparative binding studies disclosed that the apparent Kd-values (86.5 +/- 7.3 and 77.0 +/- 14.3 nM, respectively) of the [3H]B interactions with CBG and GCR did not differ (P greater than 0.3). Tritium 128-130 serpin family A member 6 Rattus norvegicus 151-154 824166-1 1976 Specific binding studies of tritium-labeled LH-RH to sheep anterior pituitary membranes at 37 degrees C showed a maximum of binding capacity of 110 +/- 20 mol/mg protein with an association rate constant of 2 +/- 1 X 10(5) M-1 S-1 and a dissociation rate constant of 0.7 +/- 0.4 X 10(-2) S-1. Tritium 28-35 Progonadoliberin-1 Ovis aries 44-49 952873-6 1976 Galactosyltransferase was inactivated with a [3H]UDP derivative and the predominant labeled peptide, from thermolysin digestion, isolated and characterized as: Ser-Gly-Lys-UDP. Tritium 46-48 N-acetyllactosaminide alpha-1,3-galactosyltransferase Bos taurus 0-21 27889577-3 2017 Previous studies in C57BL/6 mice using [3H-arachidonoyl]anandamide demonstrated deposition of tritium in thalamus and cortical areas that was blocked by treatment with an FAAH inhibitor and that was not seen in FAAH-knockout mice. Tritium 94-101 fatty acid amide hydrolase Mus musculus 171-175 1386081-13 1992 These findings and other results showing that bromosulfophthalein will completely inhibit efflux are consistent with a role for an ATPase in [3H]MTX efflux, and suggest that the process under study is the bromosulfophthalein-sensitive, ATP-dependent route responsible for the majority of [3H]MTX efflux in intact L1210 cells. Tritium 142-144 dynein, axonemal, heavy chain 8 Mus musculus 131-137 27794115-4 2016 RESULTS: 3H-THK5351 was able to bind to tau deposits in the postmortem brain with CBD. Tritium 9-11 microtubule associated protein tau Homo sapiens 40-43 55487-0 1976 3H-thymidine autoradiography of the CSF cells in cases of non-neoplastic disease. Tritium 0-2 colony stimulating factor 2 Homo sapiens 36-39 945283-2 1976 Stereospecific syntheses of tritium-labelled (2R)- and (2S)-dopamines: stereochemical course of hydroxylation of dopamine by dopamine beta-hydroxylase (E.C. Tritium 28-35 dopamine beta-hydroxylase Homo sapiens 125-150 1386081-13 1992 These findings and other results showing that bromosulfophthalein will completely inhibit efflux are consistent with a role for an ATPase in [3H]MTX efflux, and suggest that the process under study is the bromosulfophthalein-sensitive, ATP-dependent route responsible for the majority of [3H]MTX efflux in intact L1210 cells. Tritium 289-291 dynein, axonemal, heavy chain 8 Mus musculus 131-137 1320049-13 1992 During a short incubation period (3 h), the two LHRH agonists rapidly inhibited [3H]thymidine incorporation into the cells. Tritium 81-83 gonadotropin releasing hormone 1 Homo sapiens 48-52 11352-4 1976 When polar metabolites from incubation with rat liver microsomes were incubated with catechol O-methyltransferase and S-adenosyl[methyl-3 H]-L-methionine there was conversion to 3H-labeled nonpolar products. Tritium 178-180 catechol-O-methyltransferase Rattus norvegicus 85-113 51071-0 1975 3H-thymidine autoradiography of CSF cells in primary reticulum cell sarcoma of the brain. Tritium 0-2 colony stimulating factor 2 Homo sapiens 32-35 51071-1 1975 CSF cells in a case of primary reticulum cell sarcoma of the brain with diffuse subarachnoid spreading were examined by 3H-thymidine autoradiography. Tritium 120-122 colony stimulating factor 2 Homo sapiens 0-3 1165246-1 1975 The results of a double isotope experiment using 3H- and 14C-labeled leucine as precursors of protein synthesis demonstrated that the aldolase C to A subunit transition which is associated with chick skeletal muscle development involves the preferential synthesis of different aldolase isoenzymes. Tritium 49-51 aldolase A, fructose-bisphosphate Gallus gallus 134-144 27576176-3 2016 In particular, combination of the 3,5-dimethoxy-N,N-dimethylanilin-4-yl moiety and the donor part of MCAAD-3 endowed the resulting probes, 3g and 3h, with significant selectivity toward tau aggregates (selectivity for tau over Abeta = 5.7 and 3.8); they showed much higher fluorescence intensities upon binding to tau aggregates (FItau = 49 and 108) than when bound to Abeta fibrils (FIAbeta = 9 and 28). Tritium 146-148 microtubule associated protein tau Homo sapiens 186-189 27576176-3 2016 In particular, combination of the 3,5-dimethoxy-N,N-dimethylanilin-4-yl moiety and the donor part of MCAAD-3 endowed the resulting probes, 3g and 3h, with significant selectivity toward tau aggregates (selectivity for tau over Abeta = 5.7 and 3.8); they showed much higher fluorescence intensities upon binding to tau aggregates (FItau = 49 and 108) than when bound to Abeta fibrils (FIAbeta = 9 and 28). Tritium 146-148 microtubule associated protein tau Homo sapiens 218-221 27576176-3 2016 In particular, combination of the 3,5-dimethoxy-N,N-dimethylanilin-4-yl moiety and the donor part of MCAAD-3 endowed the resulting probes, 3g and 3h, with significant selectivity toward tau aggregates (selectivity for tau over Abeta = 5.7 and 3.8); they showed much higher fluorescence intensities upon binding to tau aggregates (FItau = 49 and 108) than when bound to Abeta fibrils (FIAbeta = 9 and 28). Tritium 146-148 microtubule associated protein tau Homo sapiens 218-221 27576176-4 2016 Quantitative analysis of binding affinities and fluorescence properties of 3g and 3h revealed that microenvironment-sensitive molecular rotor-like behavior, rather than binding affinity to the target, is responsible for their selective turn-on fluorescence detection of tau fibrils. Tritium 82-84 microtubule associated protein tau Homo sapiens 270-273 27576176-5 2016 Selective fluorescent labeling of tau fibrils by 3g and 3h was further demonstrated by immunofluorescence staining of human Alzheimer"s disease brain sections, which showed colocalization of the probes (3g and 3h) and phosphorylated tau antibody. Tritium 56-58 microtubule associated protein tau Homo sapiens 34-37 27576176-5 2016 Selective fluorescent labeling of tau fibrils by 3g and 3h was further demonstrated by immunofluorescence staining of human Alzheimer"s disease brain sections, which showed colocalization of the probes (3g and 3h) and phosphorylated tau antibody. Tritium 210-212 microtubule associated protein tau Homo sapiens 34-37 27655909-6 2016 However, l-Gln and 3H-labeled methyl amino isobutyrate (MeAIB) was significantly compromised in N-glycosylation-impaired mutants and deglycosylated SNAT1 when compared with the wild-type control. Tritium 19-21 solute carrier family 38 member 1 Homo sapiens 148-153 6025563-0 1967 The tritium-hydrogen exchange of myosin and its proteolytic fragments. Tritium 4-11 myosin heavy chain 14 Homo sapiens 33-39 1619025-4 1992 Both GnRH concentrations significantly increased iLH release and enhanced secreted and cellular [3H]gln-LH. Tritium 97-99 gonadotropin releasing hormone 1 Homo sapiens 5-9 26896627-8 2016 NLRP3 myocardial expression was significantly increased at 24h and 6h vs 3h (P<0.01). Tritium 73-75 NLR family, pyrin domain containing 3 Mus musculus 0-5 1322575-6 1992 In cells cotreated with TCDD plus 100 ng/ml insulin or 10 nM 17 beta-estradiol, EGF, TGF-alpha and IGF-I, TCDD caused a concentration-dependent decrease in cell proliferation and [3H]thymidine uptake. Tritium 180-182 transforming growth factor alpha Homo sapiens 85-94 33742656-4 2021 We characterised the binding of the tau tracers PI2620, RO948, MK6240 and JNJ067 in human post-mortem brain tissue from a cohort of 25 dementia cases and age-matched controls, using quantitative phosphorimaging with tritium labelled radiotracers in conjunction with phospho-tau specific immunohistochemistry. Tritium 216-223 microtubule associated protein tau Homo sapiens 36-39 26999091-6 2016 In a Madin-Darby canine kidney (MDCK)II-MDR1 cell-based transport study, Compound 3h was able to permeate the BBB-mimicking monolayer and did not result in a glycoprotein-p (P-gp) substrate, showing an efflux ratio = 0.96, close to that of diazepam. Tritium 82-84 PGP Canis lupus familiaris 174-178 1616054-4 1992 The association of [3H]SP-B with type II cells was characterized via light and electron microscopic autoradiography. Tritium 20-22 surfactant protein B Rattus norvegicus 23-27 26977299-3 2016 Therefore, the aim of this study was to determine the mass balance, pharmacokinetic, and absorption, distribution, metabolism, and excretion (ADME)-properties of tritium-labeled 2"-O-methyluridine, following a single intravenous dose to male CD-1 mice. Tritium 162-169 CD1 antigen complex Mus musculus 242-246 33374302-7 2020 Gene knockdown of LAT1 (l-type amino acid transporter 1) and CAT1 (cationic amino acid transporter 1) demonstrated that LAT1 is primarily involved in the transport of [3H]l-tryptophan in both TR-BBB and NSC-34 cell lines. Tritium 168-170 solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 Mus musculus 61-65 1616054-5 1992 Significant quantities of [3H]SP-B were found at the plasma membrane, in the endocytic pathway, and in lamellar bodies. Tritium 27-29 surfactant protein B Rattus norvegicus 30-34 33374302-7 2020 Gene knockdown of LAT1 (l-type amino acid transporter 1) and CAT1 (cationic amino acid transporter 1) demonstrated that LAT1 is primarily involved in the transport of [3H]l-tryptophan in both TR-BBB and NSC-34 cell lines. Tritium 168-170 solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 Mus musculus 67-100 1338336-4 1992 For cells testing positive for ecto-PLC activity, rapid uptake of [3H]-lysoPI is accompanied by the simultaneous appearance of [3H]-inositol phosphate in the external medium. Tritium 67-69 tripartite motif-containing 33 Mus musculus 31-35 32711231-4 2020 The most potent SIRT1 inhibitor 3h, bearing an isopropyl substituent, was as potent as EX-527, and more selective for SIRT1 over SIRT2. Tritium 32-34 sirtuin 2 Homo sapiens 129-134 26153065-4 2015 Radioligand binding with tritium-labelled diprenorphine confirmed the expression of KOP receptors. Tritium 25-32 serine peptidase inhibitor, Kunitz type 2 Homo sapiens 84-87 1338336-4 1992 For cells testing positive for ecto-PLC activity, rapid uptake of [3H]-lysoPI is accompanied by the simultaneous appearance of [3H]-inositol phosphate in the external medium. Tritium 67-69 perlecan (heparan sulfate proteoglycan 2) Mus musculus 36-39 1534919-0 1992 Thromboxane A2 receptor characterization in human astrocytoma cells and rabbit platelets by a new thromboxane antagonist, [3H]ONO NT-126. Tritium 123-125 thromboxane A2 receptor Homo sapiens 0-23 26147003-7 2015 A subsequent autoradiography was performed with [3H]DAMGO, a selective MOR agonist. Tritium 49-51 opioid receptor mu 1 Homo sapiens 71-74 32593790-5 2020 Mu-opioid receptor expression, measured by [3H] ([D-Ala2, N-MePhe4, Gly-ol]-enkephalin) (DAMGO), showed no effect of exercise while showing an effect of sex, with increased [3H] DAMGO binding in the brains of sedentary males compared to that of sedentary females. Tritium 44-46 proenkephalin Rattus norvegicus 76-86 32481639-6 2020 Moreover, the Annexin-V/PI apoptotic assay elucidate that compound 3h showed 33% and potentially 140% increase in early and late apoptosis to MCF-7 cells respectively, compared to the control. Tritium 67-69 annexin A5 Homo sapiens 14-23 26195725-6 2015 Specific binding of RvD2 to recombinant GPR18 was confirmed using a synthetic (3)H-labeled-RvD2. Tritium 78-82 G protein-coupled receptor 18 Homo sapiens 40-45 25452000-5 2014 Tritium-labeled (E,E)-1-[(3)H]-2,5-bis(4"-hydroxy-3"-carbomethoxystyryl)benzene possesses an improved level of chemical stability relative to a previously reported radioiodinated analog for radiometric quantification of Abeta plaque and tau pathology in brain tissue and in vitro studies with synthetic Abeta and tau fibrils. Tritium 0-7 microtubule associated protein tau Homo sapiens 237-240 25452000-5 2014 Tritium-labeled (E,E)-1-[(3)H]-2,5-bis(4"-hydroxy-3"-carbomethoxystyryl)benzene possesses an improved level of chemical stability relative to a previously reported radioiodinated analog for radiometric quantification of Abeta plaque and tau pathology in brain tissue and in vitro studies with synthetic Abeta and tau fibrils. Tritium 0-7 microtubule associated protein tau Homo sapiens 313-316 31842924-8 2019 RESULTS: In vitro BBB and in silico transporter studies indicated that [3H]amisulpride and [3H]haloperidol were transported by the influx transporter, OCT1, and efflux transporters MATE1 and PMAT. Tritium 72-74 solute carrier family 29 member 4 Homo sapiens 191-195 1318585-2 1992 5HT3 receptor density was determined to be 97 +/- 5 fmol/mg and 124 +/- 10 fmol/mg of protein using the commercially available 5HT3 radioligands, 3H-GR65630 and 3H-BRL43694, respectively. Tritium 146-148 5-hydroxytryptamine receptor 3A Rattus norvegicus 0-13 31469288-0 2019 [3H]UR-DEBa176: A 2,4-Diaminopyrimidine-Type Radioligand Enabling Binding Studies at the Human, Mouse, and Rat Histamine H4 Receptors. Tritium 1-3 histamine receptor H4 Rattus norvegicus 121-133 1318585-2 1992 5HT3 receptor density was determined to be 97 +/- 5 fmol/mg and 124 +/- 10 fmol/mg of protein using the commercially available 5HT3 radioligands, 3H-GR65630 and 3H-BRL43694, respectively. Tritium 161-163 5-hydroxytryptamine receptor 3A Rattus norvegicus 0-13 25041389-3 2014 In this study, [(3) H]-labeled LS-3-134 was prepared and evaluated to further characterize its use as a D3 dopamine receptor selective radioligand. Tritium 16-21 dopamine receptor D3 Homo sapiens 104-124 1323478-2 1992 Endothelin-1 (ET-1) and endothelin-3 (ET-3) produced concentration-dependent increases in [3H]thymidine incorporation (EC50 = 0.1 and 25 nM, respectively). Tritium 91-93 endothelin 3 Rattus norvegicus 24-36 1323478-2 1992 Endothelin-1 (ET-1) and endothelin-3 (ET-3) produced concentration-dependent increases in [3H]thymidine incorporation (EC50 = 0.1 and 25 nM, respectively). Tritium 91-93 endothelin 3 Rattus norvegicus 38-42 31375704-4 2019 When present during co-polymerization of Tpm3.1 with actin, 3H-ATM-3507 is incorporated into the filaments and saturates at approximately one molecule per Tpm3.1 dimer and with an apparent binding affinity of approximately 2 microM. Tritium 60-62 ATM serine/threonine kinase Homo sapiens 63-66 25546922-12 2014 The mu-opioid receptor incubation in selected brain structures was performed with a specific radioisotope [3H]DAMGO [tyrosyl-3,5,-3H(N)-D-Ala-Gly-N-methyl-Phe-Gly-enkephalin]. Tritium 107-109 proenkephalin Rattus norvegicus 163-173 31375704-5 2019 In contrast, 3H-ATM-3507 is poorly incorporated into preformed Tpm3.1/actin co-polymers. Tritium 13-15 ATM serine/threonine kinase Homo sapiens 16-19 1319105-3 1992 In 24 h cultures, cystatin C (50 micrograms/ml) significantly inhibited the release of 45Ca and 3H stimulated by parathyroid hormone (PTH, 15 nmol/liter) or parathyroid hormone-related peptide of malignancy (PTHrP, 15 nmol/liter). Tritium 96-98 cystatin C Homo sapiens 18-28 1355520-3 1992 infusion of TRH on the release and synthesis of prolactin in the rat pituitary gland and on the changes in binding of [3H]MeTRH and dopamine turnover rates in rat hypothalamus. Tritium 119-121 thyrotropin releasing hormone Rattus norvegicus 12-15 30376629-4 2018 Transcellular transport studies showed that prazosin, [14C]l-lactate, [3H]l-arginine, and [3H]l-glutamate (substrates of BCRP, MCT1, CAT1, and GLAST, respectively) were transported asymmetrically across the hiPS-BMEC monolayer. Tritium 91-93 BCR pseudogene 1 Homo sapiens 121-125 30376629-4 2018 Transcellular transport studies showed that prazosin, [14C]l-lactate, [3H]l-arginine, and [3H]l-glutamate (substrates of BCRP, MCT1, CAT1, and GLAST, respectively) were transported asymmetrically across the hiPS-BMEC monolayer. Tritium 91-93 GIT ArfGAP 1 Homo sapiens 133-137 24697602-6 2014 UDP-induced bladder hyperactvity was also prevented by blocking P2X3 and P2Y1 receptors, respectively, with A317491 and MRS2179 applied i.v.. UDP decreased [(3) H]-ACh release from stimulated bladder strips with urothelium, but not in its absence. Tritium 157-162 purinergic receptor P2X 3 Rattus norvegicus 64-68 1355520-13 1992 Moreover, exposure to TRH stimulated [3H]dopamine release from rat tuberoinfundibular neurones in a time- and dose-dependent manner. Tritium 38-40 thyrotropin releasing hormone Rattus norvegicus 22-25 24481648-7 2014 Ponatinib significantly enhances the accumulation of [3H]-paclitaxel in cells expressing MRP7. Tritium 53-57 ATP binding cassette subfamily C member 10 Homo sapiens 89-93 1515017-2 1992 Within the range of 10-1000 pg/ml, interleukin-1 beta produced over 40% of angiotensin II-stimulated [3H]thymidine incorporation (P less than 0.005 compared with basal for 10 pg/ml and 1000 pg/ml; P less than 0.05 for 100 pg/ml; two-tailed unpaired Student"s t-test). Tritium 102-104 interleukin 1 beta Bos taurus 35-53 24117008-4 2014 KEY RESULTS: The binding of [(3)H]-melatonin to the hMT1 and hMT2 receptors displayed two sites on the saturation curves. Tritium 28-35 ALG1 chitobiosyldiphosphodolichol beta-mannosyltransferase Homo sapiens 52-56 24117008-4 2014 KEY RESULTS: The binding of [(3)H]-melatonin to the hMT1 and hMT2 receptors displayed two sites on the saturation curves. Tritium 28-35 metallothionein 2A Homo sapiens 61-65 30443021-4 2018 TMEM97 KO resulted in a complete loss of binding of [125I]RHM-4 and a significant reduction in binding of [3H]DTG. Tritium 107-109 transmembrane protein 97 Homo sapiens 0-6 30443021-5 2018 TMEM97 KO and PGRMC1 KO resulted in an equal reduction in the rate of uptake of fluorescently-tagged or 3H-labeled LDL, and knocking out both proteins did not result in a further rate of reduction of LDL uptake. Tritium 104-106 transmembrane protein 97 Homo sapiens 0-6 30443021-5 2018 TMEM97 KO and PGRMC1 KO resulted in an equal reduction in the rate of uptake of fluorescently-tagged or 3H-labeled LDL, and knocking out both proteins did not result in a further rate of reduction of LDL uptake. Tritium 104-106 progesterone receptor membrane component 1 Homo sapiens 14-20 1554348-8 1992 These values were consistent with the coupling of an M3 muscarinic receptor to the release of [3H]NA in SH-SY5Y cells. Tritium 95-97 cholinergic receptor muscarinic 3 Homo sapiens 53-75 30108159-2 2018 Both [3H]BUP and [3H]SAM bound to the mu-, kappa-, and delta-opioid receptors (MOR, KOR, and DOR, respectively) with Kd values of 3 nM or less. Tritium 6-8 opioid receptor mu 1 Homo sapiens 79-82 30108159-2 2018 Both [3H]BUP and [3H]SAM bound to the mu-, kappa-, and delta-opioid receptors (MOR, KOR, and DOR, respectively) with Kd values of 3 nM or less. Tritium 18-20 opioid receptor mu 1 Homo sapiens 79-82 23867331-10 2013 The absolute value change of the hs-TnT from T0 to T3 sampling 3h later improved the ROC-AUC up to 0.972 and the specificity was 92% at 22 ng/L (ROC-optimized cut-off) and 100% at 105 ng/L (rule-in cut-off). Tritium 63-65 troponin T1, slow skeletal type Homo sapiens 36-39 24056801-4 2013 First, we demonstrate a cell-based targeted photocrosslinking technology to identify the residues in the ligand-binding pocket of GPCR where a tritium-labeled small-molecule ligand is crosslinked to a genetically-encoded azido amino acid. Tritium 143-150 C-X-C motif chemokine receptor 6 Homo sapiens 130-134 29675746-4 2018 Custom [3H]-azido-taxane analogues were synthesized for the photoaffinity labeling of P-gp. Tritium 8-10 phosphoglycolate phosphatase Homo sapiens 86-90 29353375-4 2018 A dual inhibitor of the biosynthetic enzymes for H2S, cystathionine beta-synthase (CBS) and cystathionine gamma-lyase (CSE), amino-oxyacetic acid (AOA; 3 mM) reversed the inhibitory responses caused by GYY 4137, L-cysteine and N-acetylcysteine on K+-evoked [3H]D-aspartate release. Tritium 258-260 cystathionine gamma-lyase Bos taurus 92-117 1371963-6 1992 The mean marrow transit time derived from myelocyte metamyelocyte transition time and the emergence of [3H]TdR-labeled cells into the peripheral blood was 45 h in the G-CSF group and 116 h in the controls. Tritium 104-106 colony stimulating factor 3 (granulocyte) Mus musculus 167-172 29491210-4 2018 [3H]GABA uptake by TauT-NHERF1-coexpressing oocytes and TauT-singly expressing oocytes exhibited saturable kinetics with Michaelis-Menten constant values of 0.835+-0.288 and 0.982+-0.569 mM and a maximal transport velocity of 206+-37 and 283+-28 pmol/(h oocyte), respectively. Tritium 1-3 SLC9A3 regulator 1 Rattus norvegicus 24-30 23146993-11 2013 However HBOT administered 3h after ischemia reversed these molecular events and resulted in CREB stabilization, infarct size reduction and neurobehavioral improvement. Tritium 26-28 cAMP responsive element binding protein 1 Rattus norvegicus 92-96 1733730-0 1992 Nerve growth factor enhances [3H]nicotine binding to a nicotinic cholinergic receptor on PC 12 cells. Tritium 30-32 nerve growth factor Rattus norvegicus 0-19 23347011-2 2013 An increase of [3H]-labeled inositol phosphate (IP1) formation by histamine (100 muM) or A23187 (100 nM) via the activation of phosphatidylinositol-specific phospholipase C (PI-PLC) to breakdown labeled substrate was observed, and this effect could be partially blocked by about half at 100 muM of 2-azafluorenones. Tritium 16-18 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 48-51 30213230-3 2018 [3H]BF-227 bound to alpha-Syn and amyloid beta peptide (Abeta) fibrils with affinities (KD) of 46.0 nM and 15.7 nM, respectively. Tritium 1-3 synuclein alpha Homo sapiens 20-29 1733730-2 1992 We, therefore, studied PC 12 cells incubated in the presence of nerve growth factor (NGF) and determined that specific [3H]nicotine-binding sites were induced approximately 2.5-fold in the presence of NGF (50 or 100 ng/ml). Tritium 120-122 nerve growth factor Rattus norvegicus 64-83 1733730-2 1992 We, therefore, studied PC 12 cells incubated in the presence of nerve growth factor (NGF) and determined that specific [3H]nicotine-binding sites were induced approximately 2.5-fold in the presence of NGF (50 or 100 ng/ml). Tritium 120-122 nerve growth factor Rattus norvegicus 85-88 29353278-7 2018 The IC50 value of (S)-lansoprazole against hOAT3-mediated transport of [3H]ES (0.61 +- 0.08 micromol/L) was significantly lower than that of (R)-lansoprazole (1.75 +- 0.31 micromol/L). Tritium 72-74 solute carrier family 22 member 8 Homo sapiens 43-48 1733730-2 1992 We, therefore, studied PC 12 cells incubated in the presence of nerve growth factor (NGF) and determined that specific [3H]nicotine-binding sites were induced approximately 2.5-fold in the presence of NGF (50 or 100 ng/ml). Tritium 120-122 nerve growth factor Rattus norvegicus 201-204 23221528-4 2012 The P-gp inhibitory effect of TSAHC was assessed by [3H]digoxin accumulation in the LLCPK1-MDR1 cell system. Tritium 53-55 phosphoglycolate phosphatase Homo sapiens 4-8 1304863-3 1992 The so-called muscarinic toxins (MTX1 and MTX2) have been shown to inhibit the specific binding of [3H]QNB (0.15 nM), [3H]PZ (2.5 nM) and [3H]oxoM (2 nM) to bovine cerebral cortex membranes by 60, 88 and 82% respectively. Tritium 100-102 metaxin 2 Bos taurus 42-46 1304863-3 1992 The so-called muscarinic toxins (MTX1 and MTX2) have been shown to inhibit the specific binding of [3H]QNB (0.15 nM), [3H]PZ (2.5 nM) and [3H]oxoM (2 nM) to bovine cerebral cortex membranes by 60, 88 and 82% respectively. Tritium 119-121 metaxin 2 Bos taurus 42-46 22940695-6 2012 As measured by autoradiography with [(3)H]8-hydroxy-2-(di-n-propyl) aminotetralin (8-OH-DPAT), a decrease of 5-HT(1A) receptor specific binding was observed in the posterior/dorsal region of the anterior cingulate gyrus and posterior/ventral area of the superior frontal gyrus of MPTP monkeys compared to controls. Tritium 36-41 5-hydroxytryptamine receptor 1A Homo sapiens 109-126 29304533-5 2018 Transport studies in membrane vesicles of Sf9 cells containing recombinant human MRP4 revealed that MRP4 mediates ATP-dependent transport of fluorescein- and tritium-labelled S1P. Tritium 158-165 sphingosine-1-phosphate receptor 1 Mus musculus 175-178 1304863-3 1992 The so-called muscarinic toxins (MTX1 and MTX2) have been shown to inhibit the specific binding of [3H]QNB (0.15 nM), [3H]PZ (2.5 nM) and [3H]oxoM (2 nM) to bovine cerebral cortex membranes by 60, 88 and 82% respectively. Tritium 119-121 metaxin 2 Bos taurus 42-46 1304863-5 1992 The Hill number for the [3H]PZ binding inhibition by the putative muscarinic toxin MTX2 was 0.95 suggesting homogeneity in the behaviour of the sites involved. Tritium 25-27 metaxin 2 Bos taurus 83-87 1304863-8 1992 The Ki for MTX2/[3H]PZ was 22.58 +/- 3.52 nM; for MTX2/[3H]oxoM, 144.9 +/- 21.07 nM and for MTX2/[3H]QNB, 134.98 +/- 18.35 nM. Tritium 17-19 metaxin 2 Bos taurus 11-15 28005438-11 2018 Gefitinib accelerated OATP1B3-mediated [3H]TCA uptake and inhibited OATP2B1-mediated [3H]E3S uptake. Tritium 86-88 solute carrier organic anion transporter family member 2B1 Homo sapiens 68-75 22820264-4 2012 Daily intraperitoneal injection of alpha7 nAChR agonist PNU-282987 (3-10mg/kg) for 3 days, starting from 3h after induction of ICH, significantly increased the number of surviving neurons in the central and the peripheral regions of hematoma at 3 days after ICH. Tritium 105-107 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 35-47 1304863-8 1992 The Ki for MTX2/[3H]PZ was 22.58 +/- 3.52 nM; for MTX2/[3H]oxoM, 144.9 +/- 21.07 nM and for MTX2/[3H]QNB, 134.98 +/- 18.35 nM. Tritium 16-19 metaxin 2 Bos taurus 11-15 1370604-2 1992 Both gp120 and gp160, at concentrations from 0.01 to 10 micrograms/ml, caused a progressive and significant (p less than 0.05) decrease in viable CD34+ cell count in liquid cultures supplemented with 2 ng/ml of human recombinant (r) interleukin-3 (IL-3), evaluated by means of Trypan-blue exclusion and [3H]thymidine ([3H]TdR) incorporation. Tritium 304-306 glutamyl aminopeptidase Homo sapiens 15-20 22538052-4 2012 Apparent K(m) values for the OATP1B1-mediated transport of [(3)H] substrates were determined prior to their use as probes in inhibition studies, and ranged from 0.6 to 29 muM for statins. Tritium 60-64 solute carrier organic anion transporter family member 1B1 Homo sapiens 29-36 1370604-2 1992 Both gp120 and gp160, at concentrations from 0.01 to 10 micrograms/ml, caused a progressive and significant (p less than 0.05) decrease in viable CD34+ cell count in liquid cultures supplemented with 2 ng/ml of human recombinant (r) interleukin-3 (IL-3), evaluated by means of Trypan-blue exclusion and [3H]thymidine ([3H]TdR) incorporation. Tritium 319-321 glutamyl aminopeptidase Homo sapiens 15-20 22394605-4 2012 Transporter expressing HEK293 cell lines were used with [3H]sulfobromophthalein ([3H]BSP) as substrate for OATP1B1 and OATP1B3, [3H]atorvastatin as substrate for OATP1B1, and [3H]1-methyl-4-phenylpyridinium ([3H]MPP(+)) as substrate for OCT1. Tritium 82-84 solute carrier organic anion transporter family member 1B1 Homo sapiens 107-114 22394605-5 2012 The G. biloba flavonoids showed a competitive inhibition of the OATP1B1- and OATP1B3-mediated [3H]BSP and the OATP1B1-mediated [3H]atorvastatin uptake. Tritium 95-97 solute carrier organic anion transporter family member 1B1 Homo sapiens 64-71 22394605-5 2012 The G. biloba flavonoids showed a competitive inhibition of the OATP1B1- and OATP1B3-mediated [3H]BSP and the OATP1B1-mediated [3H]atorvastatin uptake. Tritium 128-130 solute carrier organic anion transporter family member 1B1 Homo sapiens 110-117 22394605-6 2012 Quercetin was the most potent inhibitor of the OATP1B1- and OATP1B3-mediated [3H]BSP transport with K(i)-values of 8.8+-0.8muM and 7.8+-1.7muM, respectively. Tritium 78-80 solute carrier organic anion transporter family member 1B1 Homo sapiens 47-54 22394605-7 2012 For the inhibition of the OATP1B1-mediated [3H]atorvastatin transport, apigenin was the most potent inhibitor with a K(i) value of 0.6+-0.2muM. Tritium 44-46 solute carrier organic anion transporter family member 1B1 Homo sapiens 26-33 22394605-8 2012 Among the grapefruit flavonoids, naringenin was the most potent inhibitor of the OATP1B1- and OATP1B3-mediated [3H]BSP transport with IC(50)-values of 81.6+-1.1muM and 101.1+-1.1muM, respectively. Tritium 112-114 solute carrier organic anion transporter family member 1B1 Homo sapiens 81-88 29383190-7 2017 Results: Loss of Oct3-/- in primary hepatocytes resulted in significantly reduced OCT activity determined by [3H]MPP+ uptake in vivo. Tritium 110-112 solute carrier family 22 (organic cation transporter), member 3 Mus musculus 17-21 1294362-5 1992 Calcitonin (10(-7) M), as well as CGRP (10(-8) M), suppressed the stimulatory effect of TSH on 3H-thymidine incorporation. Tritium 95-97 calcitonin-related polypeptide alpha Rattus norvegicus 0-10 22261097-2 2012 The number of HPG molecules grafted per RBC was measured using tritium labeled HPGs ((3)H-HPG) of different molecular weights; the values ranged from 1 x 10(5) to 2 x 10(6) molecules per RBC. Tritium 63-70 gonadotropin releasing hormone 1 Mus musculus 14-17 1294362-5 1992 Calcitonin (10(-7) M), as well as CGRP (10(-8) M), suppressed the stimulatory effect of TSH on 3H-thymidine incorporation. Tritium 95-97 calcitonin-related polypeptide alpha Rattus norvegicus 34-38 22281494-2 2012 Pretreatment with 5mug/ml of tunicamycin or 600nM thapsigargin for 3h decreased insulin-mediated tyrosine phosphorylation of IRS-1 and glucose uptake, and increased the level of mTOR/S6K1 phosphorylation in L6 myotubes. Tritium 67-69 insulin receptor substrate 1 Homo sapiens 125-130 1374666-4 1992 Electrophoretic analysis of the total nucleic acids extracted from the different organs chased for 3 or 12 months showed all the tritium radioactivity to be present in the DNA fraction before digestion with DNase or RNase A, and to be completely absent from the DNA fraction and shifted to the RNA fraction, or to be largely destroyed by degradation, after each digestion, respectively. Tritium 129-136 ribonuclease A family member 1, pancreatic Homo sapiens 216-223 22281494-2 2012 Pretreatment with 5mug/ml of tunicamycin or 600nM thapsigargin for 3h decreased insulin-mediated tyrosine phosphorylation of IRS-1 and glucose uptake, and increased the level of mTOR/S6K1 phosphorylation in L6 myotubes. Tritium 67-69 ribosomal protein S6 kinase B1 Homo sapiens 183-187 22074644-11 2012 Finally, [(3)H]-MK-801 binding was decreased in the CPU and CA1 of TRPV1KO versus WT mice. Tritium 10-14 carbonic anhydrase 1 Mus musculus 60-63 1593845-5 1992 Exogenous TGF-beta inhibited [3H]-thymidine incorporation in a dose-dependent fashion and with high affinity (picomolar range), but with slightly lower potency in high versus normal glucose media. Tritium 30-32 transforming growth factor, beta 1 Mus musculus 10-18 23788904-5 2012 As compared to the control cultures the Pgp transport function was the most strongly inhibited by 1a, 1b, 1d, 3f, 3h and 3i analogues (approximately by 25%). Tritium 114-116 phosphoglycolate phosphatase Homo sapiens 40-43 1347404-2 1992 The stimulation-evoked overflow of tritium, representing action potential-induced, exocytotic release of 5-HT, was inhibited concentration-dependently by 5-HT receptor ligands, the potencies of which were compatible with the assumption of a 5-HT1D-like autoreceptor. Tritium 35-42 5-hydroxytryptamine receptor 1D Oryctolagus cuniculus 241-247 21854757-4 2011 Further the level of hRFT-2 mRNA expression in Caco-2 cells and in native human intestine is significantly higher than that of hRFT-1 and -3; hRFT-2 was also more efficient in transporting 3H-RF than hRFT-1 and -3. Tritium 189-191 solute carrier family 52 member 3 Homo sapiens 21-27 21854757-4 2011 Further the level of hRFT-2 mRNA expression in Caco-2 cells and in native human intestine is significantly higher than that of hRFT-1 and -3; hRFT-2 was also more efficient in transporting 3H-RF than hRFT-1 and -3. Tritium 189-191 solute carrier family 52 member 3 Homo sapiens 142-148 1665747-19 1991 We conclude that three pharmacological subtypes of alpha2-adrenoceptors are labelled by [3H]-RX821002 in the rat kidney. Tritium 89-91 adrenoceptor alpha 2A Rattus norvegicus 51-71 1935798-5 1991 Both cortisol and IL-1 alpha decreased the incorporation of [3H]proline into collagenase-digestible protein (CDP) and reduced alpha 1(I)procollagen mRNA levels at 72 h. Noncollagen protein (NCP) labeling was increased by IL-1 and decreased by cortisol. Tritium 61-63 interleukin 1 complex Mus musculus 18-22 21873053-3 2011 The resulting bioconjugates bind with high affinity and specificity to their cell-surface receptor targets in vitro and appear stable to degradation in mouse serum over 3h of incubation at 37 C. Tritium 169-171 CD177 molecule Homo sapiens 77-98 1939061-6 1991 Since only 18% of [6-3H]5-iodouracil reduced by DHPDHase was covalently bound to the enzyme and radiolabel was not lost to the solvent as tritium, the partition coefficient for inactivation was 4.5. Tritium 138-145 dihydropyrimidine dehydrogenase Bos taurus 48-56 21943380-6 2011 Furthermore, the effect of ectopic overexpression of SOX11 on proliferation was studied through [3H]-thymidine incorporation. Tritium 97-99 SRY-box transcription factor 11 Homo sapiens 53-58 1910817-5 1991 The ability of BPAEC to incorporate fatty acids or lysophosphatides into complex phospholipids is similarly impaired after exposure to Cd2+, suggesting that the liberation of [3H]AA might be due to impairment of reacylation mechanisms and not to increased hydrolytic activity of phospholipase A2. Tritium 176-178 CD2 molecule Bos taurus 135-138 1910817-7 1991 Cd2+ also causes a profound time- and dose-dependent depletion of adenosine triphosphate levels in BPAEC, and these changes closely correlate with the liberation of [3H]AA. Tritium 166-168 CD2 molecule Bos taurus 0-3 21456079-5 2011 Compound 3h which exhibited good antimicrobial activity was incorporated into SLN and characterized for particle size, entrapment efficiency (EE%), scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and in-vitro release studies. Tritium 9-11 sarcolipin Homo sapiens 78-81 1774470-5 1991 Increased [3H]TdR uptake was observed in 5 patients and was suppressed by the addition of IFN-beta. Tritium 11-13 interferon beta 1 Homo sapiens 90-98 21145044-6 2011 As the results of SQGD (30mug/ml) stimulation for 3h at 37 C, significant induction in IL-12p40, IL-23p19 and RelA gene expression was observed in PBMCs compared to unstimulated control cells. Tritium 50-52 interleukin 23 subunit alpha Homo sapiens 97-105 21282933-2 2011 NPT4 variants carrying SNP V257F, G279R, or P378L exhibited reduced transport of [(14)C]para-aminohippurate, [(3)H]bumetanide, [(3)H]estrone sulfate, and [(14)C]urate, when each variant clone was expressed in the plasma membrane of oocytes. Tritium 109-115 solute carrier family 17 member 3 Homo sapiens 0-4 20851116-5 2010 EPO significantly and concentration-dependently increased DNA synthesis as assessed by [3H]thymidine incorporation, cell proliferation as assessed by WST-1 assay, and activation of the p44/42MAPK pathway. Tritium 88-90 erythropoietin Rattus norvegicus 0-3 1680161-2 1991 The dose-response curves for the PTX-induced increases in [3H]ACh release and in [Ca2+]i were depressed by removing extracellular Ca2+ or by decreasing extracellular Na+ concentrations. Tritium 59-61 acyl-CoA thioesterase 12 Rattus norvegicus 62-65 20801125-8 2010 A two-step, sandwich-type enzymatic microplate assay has been developed to measure AMH levels in 20 muL of sample in less than 3h. Tritium 127-129 anti-Mullerian hormone Homo sapiens 83-86 1680161-3 1991 The release of [3H]ACh induced by concentrations of PTX less than 10(-10) M was more dependent on the simultaneous presence of both Ca2+ and Na+ than the release induced by higher concentrations of PTX. Tritium 16-18 acyl-CoA thioesterase 12 Rattus norvegicus 19-22 1680161-4 1991 The PTX-induced increase both in [3H]ACh release and in [Ca2+]i was almost completely abolished by the combination of Ca2+ deprivation and Na+ concentration reduction. Tritium 34-36 acyl-CoA thioesterase 12 Rattus norvegicus 37-40 20630221-13 2010 Insulin concentrations were greater in PB cows assigned to GLUC compared with SAL cohorts at 0.5 and 3h following infusion, whereas NB cows assigned to GLUC had greater insulin concentrations compared with SAL cohorts at 0.5, 1, 2, and 3h. Tritium 101-103 insulin Bos taurus 0-7 1892885-8 1991 The activity of phospholipase A in cytosol, mitochondria and microsomes isolated from choline-deficient rat liver was similar to the activity in control liver, when determined with [3H]PC vesicles as the substrate. Tritium 182-184 phospholipase A and acyltransferase 1 Rattus norvegicus 16-31 20083572-5 2010 Overexpression of StAR, but not mutated "loss-of-function" (R181L) StAR, enhanced efflux of [3H]cholesterol to apoAI, and this effect was maintained in macrophages pretreated with LDL or acetylated LDL. Tritium 93-95 steroidogenic acute regulatory protein Mus musculus 18-22 20083572-6 2010 The effect of StAR overexpression on apoAI-dependent [3H]cholesterol efflux was mimicked by non-sterol agonist, T901317, and 27-hydroxycholesterol, and blocked by LXR inhibitor, geranylgeranyl pyrophosphate, sterol 27-hydroxylase inhibitor, GW273297x, and probucol, inhibitor of ATP binding cassette transporter A1 (ABCA1). Tritium 54-56 steroidogenic acute regulatory protein Mus musculus 14-18 19800951-4 2010 In rat brain membranes Ac-RYYRIR-ol displaced both [(3)H]nociceptin/OFQ and [(3)H]Ac-RYYRIK-ol with high affinity (pK(i) 9.35 and 8.81, respectively) and stimulated [(35)S]GTPgammaS binding showing however lower maximal effects than N/OFQ (alpha=0.28). Tritium 51-56 prepronociceptin Rattus norvegicus 57-67 20816162-5 2010 T-synthase activity is commonly measured by its ability to transfer [3H]Gal from UDP-[3H]Gal to an artificial acceptor GalNAcalpha-1-O-phenyl to form [3H]Galbeta1-3GalNAcalpha-1-O-phenyl, which can then be isolated and quantified. Tritium 69-71 core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 Homo sapiens 0-10 20816162-5 2010 T-synthase activity is commonly measured by its ability to transfer [3H]Gal from UDP-[3H]Gal to an artificial acceptor GalNAcalpha-1-O-phenyl to form [3H]Galbeta1-3GalNAcalpha-1-O-phenyl, which can then be isolated and quantified. Tritium 86-88 core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 Homo sapiens 0-10 28709952-4 2017 Inhibition was tested on the antiport catalysed by hLAT1 as transport of extraliposomal [3H]histidine in exchange with intraliposomal histidine. Tritium 88-92 solute carrier family 7 member 5 Homo sapiens 51-56 28893623-5 2017 Cells were treated with varying concentrations of abiraterone for 24h and CYP21A2 activity was measured using [3H] 17-hydroxyprogesterone as substrate. Tritium 111-113 cytochrome P450 family 21 subfamily A member 2 Homo sapiens 74-81 28431350-4 2017 Multiple regression analysis revealed that a portion of Alb and Alc, which reacted with the ferron reagent during the period from 30 min to 3 h, that is, the (Al30min-3h) fraction, had the highest arsenate sorption capacity, followed by a colloidal aluminum fraction (Al>3h, which reacted with ferron at a time of >3 h). Tritium 167-169 allantoicase Homo sapiens 64-67 28431350-4 2017 Multiple regression analysis revealed that a portion of Alb and Alc, which reacted with the ferron reagent during the period from 30 min to 3 h, that is, the (Al30min-3h) fraction, had the highest arsenate sorption capacity, followed by a colloidal aluminum fraction (Al>3h, which reacted with ferron at a time of >3 h). Tritium 274-276 allantoicase Homo sapiens 64-67 1892885-9 1991 Measurement of the activity of phospholipase A with endogenously [3H]choline-labeled PC showed that the formation of lysoPC in mitochondria isolated form choline-supplemented cells was 40% lower than in choline-deficient cells. Tritium 66-68 phospholipase A and acyltransferase 1 Rattus norvegicus 31-46 28246041-9 2017 Photolabelling studies with [3H]-azidopine and molecular docking studies have identified that 2,4-dimethylacridones have potential to modulate the P-gp mediated multidrug resistance. Tritium 29-31 phosphoglycolate phosphatase Homo sapiens 147-151 19787211-6 2009 We determined 3H-testosterone uptake in the myosin knocked-down LNCaP cells. Tritium 14-16 myosin heavy chain 14 Homo sapiens 44-50 1651844-1 1991 Treatment of pituitary GH4C1 cells with epidermal growth factor (EGF) caused up to a 60% reduction in the amount of [3H]MeTRH bound to specific TRH receptors. Tritium 117-119 epidermal growth factor like 1 Rattus norvegicus 40-63 19405101-3 2009 NIH3T3ChAT 112-1 cells showed increased binding of the CHT1 inhibitor [(3)H]hemicholinium-3 (HC-3) and greater [(3)H]choline uptake and ACh synthesis than NIH3T3ChAT 103-1 cells, a CHT1-negative control cell line. Tritium 70-76 solute carrier family 5 (choline transporter), member 7 Mus musculus 55-59 27702575-4 2017 In neurons cultured for 3 days, 8-OH-DPAT treatment for both 3h and 24h increased the mRNA levels of BDNF and GluR1, but not GluR2. Tritium 61-63 brain derived neurotrophic factor Mus musculus 101-105 1651844-1 1991 Treatment of pituitary GH4C1 cells with epidermal growth factor (EGF) caused up to a 60% reduction in the amount of [3H]MeTRH bound to specific TRH receptors. Tritium 117-119 epidermal growth factor like 1 Rattus norvegicus 65-68 27141878-11 2017 Compound 3h negatively regulated activity of sphingosine kinase 1 in HeLa cells. Tritium 9-11 sphingosine kinase 1 Homo sapiens 45-65 1651844-1 1991 Treatment of pituitary GH4C1 cells with epidermal growth factor (EGF) caused up to a 60% reduction in the amount of [3H]MeTRH bound to specific TRH receptors. Tritium 117-119 thyrotropin releasing hormone Rattus norvegicus 122-125 19420288-8 2009 3H-thymidine incorporation assay showed that SU5416 blocked the actions of both exogenous and endogenous VEGF on the proliferation of HRPTEC. Tritium 0-2 vascular endothelial growth factor A Rattus norvegicus 105-109 1748404-2 1991 At the presence of 20 mmol/L NH4Cl, the SOKT1-RTA conjugate killed 82% of the human peripheral blood T cells (PBT), and decreased their 3H-Leu incorporation to 6.2% of the untreated controls. Tritium 136-138 RNA binding fox-1 homolog 2 Homo sapiens 46-49 20387628-6 2009 Anti-CD40-induced B lymphocyte proliferation studied by [3H]thymidine incorporation was increased upon alpha7 nAChR inhibition with methyllicaconitine, choline or antibiotic gentamicin, as well as in the presence of the inhibitor of acetylcholine synthesis hemicholine-3. Tritium 57-59 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 103-115 19008332-3 2009 To determine whether osteoblasts possess the enzymes required to bioconvert a progesterone receptor (PR) agonist into A-ring reduced metabolites with affinity to bind estrogen receptor (ER), we studied the in vitro metabolism of [(3)H]-labeled NET in cultured neonatal rat osteoblasts and the interaction of its metabolic conversion products with cytosolic -osteoblast ER, employing a competition analysis. Tritium 230-234 estrogen receptor 1 Rattus norvegicus 186-188 19053588-1 2009 The reversible deprotonation of 3(2H)-furanone (3H-O) and 3(2H)-thiophenone (3H-S) by a series of delocalized carbanions and by CN(-), and the identity proton transfer of 3H-O to its conjugate base (3(-)-O) and of 3H-S to 3(-)-S have been studied at the MP2//6-31+G** level. Tritium 77-81 tryptase pseudogene 1 Homo sapiens 254-257 28566636-6 2017 In this study, [3H]MTX uptake was observed by using human OAT1 and OAT3-overexpressing HEK293 cells in the presence or absence of NSAIDs-Glu. Tritium 16-18 solute carrier family 22 member 6 Homo sapiens 58-62 28566636-6 2017 In this study, [3H]MTX uptake was observed by using human OAT1 and OAT3-overexpressing HEK293 cells in the presence or absence of NSAIDs-Glu. Tritium 16-18 solute carrier family 22 member 8 Homo sapiens 67-71 27760753-10 2016 Application of MLCK inhibitors to ischemia-injured porcine ileal mucosa induced significant increases in TER and reduced mucosal-to-serosal fluxes of 3H-labeled mannitol. Tritium 150-152 myosin light chain kinase Homo sapiens 15-19 27594697-8 2016 Thus, we tested and validated the hypothesis that selective uptake of HexHDL-[3H]CE by Chinese Hamster Ovary cells expressing SR-B1 is less than that of HDL-[3H]CE; thus, selective SR-B1 uptake of HDL-CE depends on HDL instability. Tritium 78-80 scavenger receptor class B member 1 Cricetulus griseus 126-131 18501699-2 2008 Rat Slc6a6/TauT-transfected HeLa cells exhibited Na(+)-, Cl(-)-, and concentration-dependent [3H]GABA uptake with a Km of 1.5 mM. Tritium 94-96 solute carrier family 6 member 6 Rattus norvegicus 4-10 1650214-15 1991 The [3H]TCDD.Ah receptor complex extracted from nuclei of Mz-Hep-1 cells incubated with [3H]TCDD in culture at 37 degrees sedimented at approximately 6 S under conditions of high ionic strength. Tritium 5-7 aryl hydrocarbon receptor Homo sapiens 13-24 18501699-3 2008 Taurine, beta-alanine, and GABA markedly inhibited Slc6a6/TauT-mediated uptake of [3H]GABA. Tritium 83-85 solute carrier family 6 member 6 Rattus norvegicus 51-57 27594697-8 2016 Thus, we tested and validated the hypothesis that selective uptake of HexHDL-[3H]CE by Chinese Hamster Ovary cells expressing SR-B1 is less than that of HDL-[3H]CE; thus, selective SR-B1 uptake of HDL-CE depends on HDL instability. Tritium 78-80 scavenger receptor class B member 1 Cricetulus griseus 181-186 1650214-15 1991 The [3H]TCDD.Ah receptor complex extracted from nuclei of Mz-Hep-1 cells incubated with [3H]TCDD in culture at 37 degrees sedimented at approximately 6 S under conditions of high ionic strength. Tritium 89-91 aryl hydrocarbon receptor Homo sapiens 13-24 1711984-4 1991 MAb IVd4 blocked binding of [3H]hyaluronan to brain HABP and to simian virus-transformed 3T3 cells, indicating a possible relationship with the 85-kDa hyaluronan receptor of these cells. Tritium 29-31 hyaluronic acid binding protein 2 Mus musculus 52-56 27617342-5 2016 Relative oral bioavailability (RBA) of the BaP from soil was estimated by comparing the area under the curve (AUC) for 3H concentration versus time in blood with the AUC observed from the same PAH mixture dosed in a food matrix. Tritium 119-121 prohibitin 2 Rattus norvegicus 43-46 27460209-2 2016 Subsequently a ring cyclization strategy was utilized to improve ADME properties leading to the discovery of a series of 1H-imidazo[4,5-c]pyridin-2(3H)-one drug like Alk5 inhibitors. Tritium 148-150 transforming growth factor beta receptor 1 Homo sapiens 166-170 18655784-0 2008 [3H]BMS-599240--a novel tritiated ligand for the characterization of BACE1 inhibitors. Tritium 1-3 beta-site APP cleaving enzyme 1 Mus musculus 69-74 1675425-2 1991 We also show that X. laevis oocytes injected with RNA encoding the rat erythroid/brain glucose transport protein (GLUT1) and expressing the corresponding functional transporter exhibit a lower accumulation of [3H]vinblastine and show a greater capacity to extrude the drug than do control oocytes not expressing the rat GLUT1 protein. Tritium 210-212 solute carrier family 2 member 1 Rattus norvegicus 114-119 18572425-3 2008 Rats were injected with different doses (0, 0.05, 0.5 or 5 microg/0.5 microl) of [3H]-[D-Ala2,MePhe4,Gly-ol5]-enkephalin (DAMGO) into the dorsal striatum daily (Exp. Tritium 82-84 proenkephalin Rattus norvegicus 110-120 18355801-6 2008 In addition, aspirin reduces the capacity of unlabeled bradykinin or the B2 receptor antagonist icatibant to destabilize pre-formed [3H]-bradykinin-receptor complexes. Tritium 133-135 bradykinin receptor B2 Homo sapiens 73-84 1648177-9 1991 In situ hybridizations reveal a pattern of GluR6 gene expression reminiscent of the binding pattern obtained with [3H]kainate. Tritium 115-117 glutamate ionotropic receptor kainate type subunit 2 Rattus norvegicus 43-48 18282641-6 2008 The anesthetic-induced [3H]5-HT release depends on the ryanodine-sensitive intracellular calcium store since it was blocked by dantrolene and azumolene (inhibitors of the calcium-release through ryanodine receptors) but was not affected by aminoethoxydiphenylborate (2-APB), an inhibitor of inositol 1,4,5-triphosphate receptor. Tritium 24-26 arginyl aminopeptidase Rattus norvegicus 269-272 26305376-4 2015 Co-expression of DAT and DJ-1 in HEK-293T cells leads to an increase in [3H] dopamine uptake that does not appear to be mediated by increased total DAT expression but rather through an increase in DAT cell surface localization. Tritium 73-75 Parkinsonism associated deglycase Homo sapiens 25-29 26305376-7 2015 Co-expression of a mini-gene that can disrupt the DAT/DJ-1 complex appears to block the increase in [3H] dopamine uptake by DJ-1. Tritium 101-103 Parkinsonism associated deglycase Homo sapiens 54-58 26305376-7 2015 Co-expression of a mini-gene that can disrupt the DAT/DJ-1 complex appears to block the increase in [3H] dopamine uptake by DJ-1. Tritium 101-103 Parkinsonism associated deglycase Homo sapiens 124-128 1903729-4 1991 The ability of hCG, human FSH, and/or IGF-I to induce aromatase activity was assayed by the stereospecific release of tritium from [1B-3H]androstenedione. Tritium 118-125 chorionic gonadotropin subunit beta 5 Homo sapiens 15-18 1654803-9 1991 These studies demonstrate the utility of [3H]TCDD and [125I]DBDD as photoaffinity labels for the Ah receptor and illustrate the structural and photochemical differences between the rodent and the human nuclear Ah receptor complexes. Tritium 42-44 aryl hydrocarbon receptor Homo sapiens 97-108 18094327-7 2008 3H-serotonin and ATP secretion were greatly reduced in SG-/- platelets. Tritium 0-2 serglycin Mus musculus 55-57 26081760-4 2015 In addition, our biological results indicated that 3d and 3h could suppress RANKL-induced osteoclastogenesis-related marker genes, such as NFATc1, c-fos, TRAP, and cathepsin K. Tritium 58-60 cathepsin K Homo sapiens 164-175 1654803-9 1991 These studies demonstrate the utility of [3H]TCDD and [125I]DBDD as photoaffinity labels for the Ah receptor and illustrate the structural and photochemical differences between the rodent and the human nuclear Ah receptor complexes. Tritium 42-44 aryl hydrocarbon receptor Homo sapiens 210-221 1902169-12 1991 In bones labeled with [3H]AA, IL-1 and PTH increased [3H]PGE2 and [3H]6-keto-PGF1 alpha release, as measured by HPLC and TLC. Tritium 23-25 interleukin 1 complex Mus musculus 30-34 25777939-11 2015 However, sulfite inhibited the activities of GPx, GST and G6PDH when cortical slices were exposed for 3h to sulfite. Tritium 102-104 hematopoietic prostaglandin D synthase Rattus norvegicus 50-53 26031461-3 2015 To this end, the current study determined the disposition and metabolism of DM1 following single intravenous administration of [(3)H]-DM1 in Sprague Dawley rats. Tritium 127-133 immunoglobulin heavy diversity 1-7 Homo sapiens 76-79 26031461-3 2015 To this end, the current study determined the disposition and metabolism of DM1 following single intravenous administration of [(3)H]-DM1 in Sprague Dawley rats. Tritium 127-133 immunoglobulin heavy diversity 1-7 Homo sapiens 134-137 17707505-4 2008 At < or =200 nM, H1 long-forms (H1b or H1c possessing coiled-coil (CC) domains) and short-forms (H1a or H1EVH1 lacking CC domains) enhance specific [3H]Ry binding to RyR1. Tritium 152-154 H1.2 linker histone, cluster member Homo sapiens 42-45 17707505-6 2008 Importantly, the combinations of H1c+H1EVH1, or H1b+H1a acted in an additive manner to enhance or inhibit [3H]Ry-binding activity. Tritium 107-109 H1.2 linker histone, cluster member Homo sapiens 33-36 18261865-5 2008 Steroid sulfatase (STS) activity was evaluated by incubating homogenized breast tissue with [3H]-estrone sulfate. Tritium 93-95 steroid sulfatase Homo sapiens 0-17 17586510-7 2008 A single 8 mg/kg infusion of lipid-free apoA-I administered 3h post-collar insertion reduced neutrophil recruitment into the vessel wall, and MPO expression, as well as endothelial expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) by >85% (p<0.01 for all). Tritium 60-62 vascular cell adhesion protein 1 Oryctolagus cuniculus 230-236 17586510-7 2008 A single 8 mg/kg infusion of lipid-free apoA-I administered 3h post-collar insertion reduced neutrophil recruitment into the vessel wall, and MPO expression, as well as endothelial expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) by >85% (p<0.01 for all). Tritium 60-62 ICAM-1 Oryctolagus cuniculus 242-275 24373771-6 2015 RESULTS: In both trials, serum IL-6 was significantly elevated (p<0.05) immediately post-exercise, while serum hepcidin was significantly elevated (p<0.05) 3h post-exercise, with no significant differences recorded between trials. Tritium 162-164 hepcidin antimicrobial peptide Homo sapiens 114-122 1902169-12 1991 In bones labeled with [3H]AA, IL-1 and PTH increased [3H]PGE2 and [3H]6-keto-PGF1 alpha release, as measured by HPLC and TLC. Tritium 23-25 parathyroid hormone Mus musculus 39-42 25016575-10 2014 In parallel, 3h after antioxidants incubation, we have observed a decrease in SOCS1 and SOCS3 protein levels, which seems time-related to antioxidant treatment. Tritium 13-15 suppressor of cytokine signaling 1 Homo sapiens 78-83 25016214-4 2014 Microarray studies from lung homogenates of mice exposed to only 3h of hypoxia revealed endothelin-1 (ET-1) and connective tissue growth factor (CTGF) as the most upregulated genes, and the mitogen-activated protein kinase (MAPK) pathway as the most differentially regulated pathway. Tritium 65-67 endothelin 1 Mus musculus 88-100 17586510-7 2008 A single 8 mg/kg infusion of lipid-free apoA-I administered 3h post-collar insertion reduced neutrophil recruitment into the vessel wall, and MPO expression, as well as endothelial expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) by >85% (p<0.01 for all). Tritium 60-62 ICAM-1 Oryctolagus cuniculus 277-283 1902169-12 1991 In bones labeled with [3H]AA, IL-1 and PTH increased [3H]PGE2 and [3H]6-keto-PGF1 alpha release, as measured by HPLC and TLC. Tritium 54-56 interleukin 1 complex Mus musculus 30-34 24884070-0 2014 Histamine H3 receptor activation counteracts adenosine A2A receptor-mediated enhancement of depolarization-evoked [3H]-GABA release from rat globus pallidus synaptosomes. Tritium 115-117 histamine receptor H3 Rattus norvegicus 0-21 1902169-12 1991 In bones labeled with [3H]AA, IL-1 and PTH increased [3H]PGE2 and [3H]6-keto-PGF1 alpha release, as measured by HPLC and TLC. Tritium 54-56 parathyroid hormone Mus musculus 39-42 17855655-5 2007 Labeling studies using [3H]dThd showed that the cells expressing the mitochondrial enzyme had an increased incorporation of [3H]dThd into DNA, shown to be due to a higher [3H]dTTP specific activity of the total dTTP pool in the cells in which Dm-dNK was targeted to the mitochondria. Tritium 24-26 deoxyribonucleoside kinase Drosophila melanogaster 243-249 1902169-12 1991 In bones labeled with [3H]AA, IL-1 and PTH increased [3H]PGE2 and [3H]6-keto-PGF1 alpha release, as measured by HPLC and TLC. Tritium 54-56 interleukin 1 complex Mus musculus 30-34 17855655-5 2007 Labeling studies using [3H]dThd showed that the cells expressing the mitochondrial enzyme had an increased incorporation of [3H]dThd into DNA, shown to be due to a higher [3H]dTTP specific activity of the total dTTP pool in the cells in which Dm-dNK was targeted to the mitochondria. Tritium 125-127 deoxyribonucleoside kinase Drosophila melanogaster 243-249 24264046-7 2014 The [(3)H]riboflavin uptake from the apical side of T84 cells was prominently inhibited by the RFVT3 selective inhibitor methylene blue and significantly decreased by transfection of RFVT3-small-interfering RNA. Tritium 5-9 solute carrier family 52 member 3 Homo sapiens 95-100 1902169-12 1991 In bones labeled with [3H]AA, IL-1 and PTH increased [3H]PGE2 and [3H]6-keto-PGF1 alpha release, as measured by HPLC and TLC. Tritium 54-56 parathyroid hormone Mus musculus 39-42 24264046-7 2014 The [(3)H]riboflavin uptake from the apical side of T84 cells was prominently inhibited by the RFVT3 selective inhibitor methylene blue and significantly decreased by transfection of RFVT3-small-interfering RNA. Tritium 5-9 solute carrier family 52 member 3 Homo sapiens 183-188 1902169-13 1991 IL-1 slightly increased [3H]AA release, but PTH did not. Tritium 25-27 interleukin 1 complex Mus musculus 0-4 1708783-8 1991 As with serum, epidermal growth factor and transforming growth factor alpha (TGF alpha) (10 pM to 1 nM), insulin (10 nM to 1 microM) and insulinlike growth factor-I (IGF-I, 1-100 nM) increased [3H]-thymidine uptake. Tritium 194-196 insulin like growth factor 1 Canis lupus familiaris 137-164 23417430-9 2013 3h significantly reduced total ganglioside sialic acid, GM2, and GA2 content in cerebrum, cerebellum and liver of Sandhoff mice. Tritium 0-2 cytochrome b5 domain containing 2 Mus musculus 56-59 17898208-7 2007 Analysis of the in vitro binding studies indicated that [3H]-PIB binds to alpha-synuclein fibrils but with lower affinity than that demonstrated/reported for Abeta(1-42) fibrils. Tritium 57-59 synuclein alpha Homo sapiens 74-89 17631923-9 2007 Deletion of alpha4 virtually eliminated cytisine-sensitive, higher-affinity [3H]epibatidine binding as did beta2 deletion, confirming that these sites are alpha4beta2*-nAChR. Tritium 77-79 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 168-173 1658766-6 1991 While the binding of [3H][3-Me-His2]TRH to the rat anterior pituitary, hypothalamus, cortex and brainstem homogenates was saturable and of high affinity, no saturable binding was observed in the duodenal smooth muscle. Tritium 22-24 thyrotropin releasing hormone Rattus norvegicus 36-39 17329004-2 2007 TUBU (10 microM, for 3h) treatment resulted in rapid and sustained phosphorylation of Cdc25C (Ser-216) leading to increased 14-3-3beta binding. Tritium 21-23 cell division cycle 25C Homo sapiens 86-92 23202986-2 2013 In this work, we find that this coefficient strongly correlates with the temperature coefficient of the through-hydrogen-bond coupling, (3h)J(NC"), based on NMR measurements of protein GB3. Tritium 137-139 alpha 1,4-galactosyltransferase (P blood group) Homo sapiens 185-188 23123743-2 2013 17beta-Estradiol (E2) and ESR1-selective agonist PPT, but not ESR2-selective agonist DPN, induced a rapid increase on total [3H]-inositol phosphate accumulation in the hippocampus from both rats. Tritium 125-127 estrogen receptor 1 Rattus norvegicus 26-30 23123743-4 2013 The pretreatment with ESR1 and ESR2 antagonist ICI 182,780, but not with GPER antagonist G-15, blocked the total [3H]-inositol phosphate accumulation induced by E2 and PPT, confirming that ESR1 is upstream component regulating this rapid effect. Tritium 114-117 estrogen receptor 1 Rattus norvegicus 22-26 23123743-4 2013 The pretreatment with ESR1 and ESR2 antagonist ICI 182,780, but not with GPER antagonist G-15, blocked the total [3H]-inositol phosphate accumulation induced by E2 and PPT, confirming that ESR1 is upstream component regulating this rapid effect. Tritium 114-117 estrogen receptor 2 Rattus norvegicus 31-35 23123743-5 2013 SRC family of protein tyrosine kinases inhibitor PP2 blocked the total [3H]-inositol phosphate accumulation induced by E2 and PPT in hippocampus, suggesting that ESR1 undergoes translocation from the nuclei to the plasma membrane region via SRC to activate rapid signaling pathways. Tritium 71-75 estrogen receptor 1 Rattus norvegicus 162-166 17706920-4 2007 The time and dose of BPA exposure were set after semiquantitative (sq) RT-PCR analysis of response of candidate genes (StAR, Cyp 17 a1 and AR) to 3h at 10 microg/l hCG +/- 10(-5)M BPA. Tritium 146-148 steroidogenic acute regulatory protein Mus musculus 119-123 17606337-5 2007 As(3+) potentiated the induction of Cyp1a1 mRNA by TCDD only at 3h and 6h after treatment. Tritium 64-66 cytochrome P450, family 1, subfamily a, polypeptide 1 Mus musculus 36-42 2016859-3 1991 TGF-beta inhibited both baseline and EGF-stimulated [3H]thymidine incorporation after 48 hours of exposure but enhanced EGF-stimulated DNA synthesis at 24 hours. Tritium 53-55 pro-epidermal growth factor Oryctolagus cuniculus 37-40 17628607-9 2007 [(3)H]-phenylalanine uptake into cardiomyocytes, myofilament reorganization, and cardiomyocyte size were also augmented with neurotrophin-3 stimulation, indicating that neurotrophin-3 is a novel cardiac hypertrophic factor. Tritium 0-5 neurotrophin 3 Rattus norvegicus 125-139 17628607-9 2007 [(3)H]-phenylalanine uptake into cardiomyocytes, myofilament reorganization, and cardiomyocyte size were also augmented with neurotrophin-3 stimulation, indicating that neurotrophin-3 is a novel cardiac hypertrophic factor. Tritium 0-5 neurotrophin 3 Rattus norvegicus 169-183 22843228-7 2012 The FA-inhibited [3H]thymidine incorporation was completely blocked when the expressions of p21 and p27 were knocked-down together. Tritium 18-20 H3 histone pseudogene 16 Homo sapiens 92-95 22843228-7 2012 The FA-inhibited [3H]thymidine incorporation was completely blocked when the expressions of p21 and p27 were knocked-down together. Tritium 18-20 interferon alpha inducible protein 27 Homo sapiens 100-103 22683359-8 2012 Ca(++)/calmodulin and protein kinase A (PKA) pathways are found to play an important role in the intracellular regulation of [3H]-riboflavin. Tritium 126-128 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 22-38 22683359-8 2012 Ca(++)/calmodulin and protein kinase A (PKA) pathways are found to play an important role in the intracellular regulation of [3H]-riboflavin. Tritium 126-128 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 40-43 1830134-2 1991 ([3H]8-OH-DPAT), a serotonergic agonist, to 5-HT1A receptors was determined in membranes from the brain of the rat. Tritium 2-4 5-hydroxytryptamine receptor 1A Rattus norvegicus 44-50 22394605-4 2012 Transporter expressing HEK293 cell lines were used with [3H]sulfobromophthalein ([3H]BSP) as substrate for OATP1B1 and OATP1B3, [3H]atorvastatin as substrate for OATP1B1, and [3H]1-methyl-4-phenylpyridinium ([3H]MPP(+)) as substrate for OCT1. Tritium 57-59 solute carrier organic anion transporter family member 1B1 Homo sapiens 107-114 21929836-3 2012 Conversely to NBD-cholesterol, the uptakes of [3H]cholesterol and tocopherol by Caco-2 cells were impaired by both block lipid transport-1 and ezetimibe, which inhibit SR-BI and NPC1L1, respectively. Tritium 46-49 NPC1 like intracellular cholesterol transporter 1 Homo sapiens 178-184 17437547-5 2007 Retigabine-induced inhibition of K+-evoked [3H]DA release was also abolished by synaptosomal entrapment of blocking anti-KCNQ2 polyclonal antibodies, an effect prevented by antibody pre-absorption with the KCNQ2 immunizing peptide. Tritium 44-46 potassium voltage-gated channel subfamily Q member 2 Rattus norvegicus 121-126 1946036-2 1991 [3H]5-HT binding to 5-HT1A and non-5-HT1A sites, of high and low affinity for spiperone, respectively, was investigated in three parts of the hippocampus: dorsal, medial and ventral. Tritium 1-3 5-hydroxytryptamine receptor 1A Rattus norvegicus 20-26 17258238-6 2007 The administration of E2 (1mg/kg) 3h before ischemia was able to protect CA1 neurons from these ischemia-induced synaptic dysfunctions. Tritium 34-36 carbonic anhydrase 1 Rattus norvegicus 73-76 16426477-3 2007 PMS777 (1-100 microM) dose-dependently inhibited PAF-induced rabbit platelet aggregation by competing with [3H]PAF for its receptor on platelets, and protected a human neuroblastoma cell line SH-SY5Y against PAF-induced neurotoxicity. Tritium 108-110 PCNA clamp associated factor Homo sapiens 49-52 16426477-3 2007 PMS777 (1-100 microM) dose-dependently inhibited PAF-induced rabbit platelet aggregation by competing with [3H]PAF for its receptor on platelets, and protected a human neuroblastoma cell line SH-SY5Y against PAF-induced neurotoxicity. Tritium 108-110 PCNA clamp associated factor Homo sapiens 111-114 22270541-4 2012 beta-AR-stimulation (isoproterenol; 3h) significantly increased expression of ER stress proteins, such as GRP-78, Gadd-153, and Gadd-34, while activating caspase-12 in ARVMs. Tritium 36-38 DNA-damage inducible transcript 3 Rattus norvegicus 114-122 22117525-2 2012 At a concentration of 100 muM, 42 natural products inhibited OATP1B1-mediated [(3)H]E3S uptake by more than 50%, and five of them significantly inhibited OATP1B1-mediated [(3)H]E3S by more than 80% with the following rank order of potency: quercetin > astragaloside IV > icariin > glycyrrhizic acid > ginsenoside Rc. Tritium 80-83 solute carrier organic anion transporter family member 1B1 Homo sapiens 61-68 16426477-3 2007 PMS777 (1-100 microM) dose-dependently inhibited PAF-induced rabbit platelet aggregation by competing with [3H]PAF for its receptor on platelets, and protected a human neuroblastoma cell line SH-SY5Y against PAF-induced neurotoxicity. Tritium 108-110 PCNA clamp associated factor Homo sapiens 111-114 1946036-2 1991 [3H]5-HT binding to 5-HT1A and non-5-HT1A sites, of high and low affinity for spiperone, respectively, was investigated in three parts of the hippocampus: dorsal, medial and ventral. Tritium 1-3 5-hydroxytryptamine receptor 1A Rattus norvegicus 35-41 1946036-4 1991 [3H]5-HT uptake and [3H]5-HT binding to 5-HT1A and non-5-HT1A sites were significantly higher in the ventral than in the dorsal part of the hippocampus. Tritium 21-23 5-hydroxytryptamine receptor 1A Rattus norvegicus 40-46 1946036-4 1991 [3H]5-HT uptake and [3H]5-HT binding to 5-HT1A and non-5-HT1A sites were significantly higher in the ventral than in the dorsal part of the hippocampus. Tritium 21-23 5-hydroxytryptamine receptor 1A Rattus norvegicus 55-61 1946036-5 1991 The dorso-ventral hippocampal gradient in [3H]5-HT binding to both 5-HT1A and non-5-HT1A sites was due to an increase of the density (Bmax) of these sites along the longitudinal hippocampal axis, but not their affinity (Kd) for [3H]5-HT. Tritium 43-45 5-hydroxytryptamine receptor 1A Rattus norvegicus 67-73 17177511-3 2006 The 100% methanol, 75% ethanol, and 40% 2-propanol extracts of black cohosh effectively displaced the specific binding of [3H]DAMGO to hMOR. Tritium 123-125 opioid receptor mu 1 Homo sapiens 135-139 17177511-4 2006 Further studies of the clinically used ethanol extract indicated that black cohosh acted as a mixed competitive ligand, displacing 77 +/- 4% [3H]DAMGO to hMOR (Ki = 62.9 microg/mL). Tritium 142-144 opioid receptor mu 1 Homo sapiens 154-158 17331325-4 2006 However, haloperidol was found to have an inhibitory effect on the MDR1-mediated transport of [(3)H]digoxin and [(3)H]vinblastine with an IC50 value of 7.84+/-0.76 and 3.60+/-0.64 microM, respectively, suggesting that the intestinal absorption, not distribution into the brain, of MDR1 substrate drugs could be altered by the co-administration of haloperidol in the clinical setting, although further clinical studies are needed. Tritium 94-99 ATP-binding cassette, sub-family B (MDR/TAP), member 1 Sus scrofa 67-71 17331325-4 2006 However, haloperidol was found to have an inhibitory effect on the MDR1-mediated transport of [(3)H]digoxin and [(3)H]vinblastine with an IC50 value of 7.84+/-0.76 and 3.60+/-0.64 microM, respectively, suggesting that the intestinal absorption, not distribution into the brain, of MDR1 substrate drugs could be altered by the co-administration of haloperidol in the clinical setting, although further clinical studies are needed. Tritium 94-99 ATP-binding cassette, sub-family B (MDR/TAP), member 1 Sus scrofa 281-285 17079869-7 2006 U0126, a MEK inhibitor, SP600125, a JNK inhibitor, or SB203580, a p38 inhibitor, attenuates the Ang II-induced [3H]thymidine incorporation into the VSMC. Tritium 112-114 mitogen-activated protein kinase 8 Rattus norvegicus 36-39 22380043-2 2012 The ground states of TiF(3), CrF(3), and NiF(3) are nondegenerate and exhibit minima of D(3h) symmetry. Tritium 90-92 CTD small phosphatase 1 Homo sapiens 41-47 21734190-6 2011 The involvement of GS and GLNase in the conversion of (3)H-labeled substrates was verified using irreversible pharmacological inhibitors for each of the enzymes and additionally validated by measuring intracellular amino acid levels using an HPLC. Tritium 54-58 glutaminase Rattus norvegicus 26-32 21295853-4 2011 In these studies, [3H]-LY686017 bound with a distribution and pharmacology consistent with the NK-1 receptor. Tritium 19-21 substance-P receptor Cavia porcellus 95-108 1946036-5 1991 The dorso-ventral hippocampal gradient in [3H]5-HT binding to both 5-HT1A and non-5-HT1A sites was due to an increase of the density (Bmax) of these sites along the longitudinal hippocampal axis, but not their affinity (Kd) for [3H]5-HT. Tritium 43-45 5-hydroxytryptamine receptor 1A Rattus norvegicus 82-88 1946036-5 1991 The dorso-ventral hippocampal gradient in [3H]5-HT binding to both 5-HT1A and non-5-HT1A sites was due to an increase of the density (Bmax) of these sites along the longitudinal hippocampal axis, but not their affinity (Kd) for [3H]5-HT. Tritium 229-231 5-hydroxytryptamine receptor 1A Rattus norvegicus 67-73 2054651-3 1991 CaM antagonists, including trifluoperazine (TFP), W-5, W-7, promethazine and haloperidol, dose-dependently inhibited potassium depolarization-stimulated [3H]HCh-3 binding with IC50s of 20, 40, 70, 30 and 48 (microM), respectively. Tritium 154-156 calmodulin 1 Rattus norvegicus 0-3 21130866-3 2011 H(2)O(2) (5-50muM) caused rapid activation (1-3h) of the Fas death receptor pathway of apoptosis, which was evident by up-regulation of the death ligand FasL and recruitment of the adaptor protein Fas-associated death domain to the plasma membrane. Tritium 46-48 Fas ligand Homo sapiens 153-157 17107593-4 2006 Saturation binding assay using [3H]ZM 241385, a selective A2A antagonist, as radioligand revealed a significant increase in total adenosine A2AR numbers (Bmax) in frontal cortex from PiD samples (191% of control Bmax), suggesting up-regulation of this receptor. Tritium 32-34 adenosine A2a receptor Homo sapiens 140-144 1705563-3 1991 Thyrotropin-releasing hormone (TRH), tetradecanoylphorbol acetate (TPA), and epidermal growth factor (EGF), each known to enhance calcium entry in GH4 cells, decrease GH4 cell number and incorporation of [3H]-thymidine. Tritium 205-208 thyrotropin releasing hormone Rattus norvegicus 0-29 17283870-5 2006 On interaction with mox-LDL or H2O2, U-II induced the greatest increase in [3H]thymidine incorporation among these vasoactive agents. Tritium 76-78 urotensin 2 Homo sapiens 37-41 21114976-6 2011 Isoprenaline and the selective beta(2)-adrenoceptor agonist formoterol enhanced tritium overflow independently of alpha(2)-autoinhibition in the vein. Tritium 80-87 adrenoceptor beta 2 Rattus norvegicus 31-51 1705563-3 1991 Thyrotropin-releasing hormone (TRH), tetradecanoylphorbol acetate (TPA), and epidermal growth factor (EGF), each known to enhance calcium entry in GH4 cells, decrease GH4 cell number and incorporation of [3H]-thymidine. Tritium 205-208 thyrotropin releasing hormone Rattus norvegicus 31-34 20837679-7 2010 However, [(3)H]UBP310 also binds to GluK3 (K(D) = 0.65 +- 0.19 muM) but with ~30-fold lower affinity than that observed for GluK1. Tritium 10-14 glutamate ionotropic receptor kainate type subunit 3 Homo sapiens 36-41 16920085-5 2006 The [3H]CGP-12177 bindings to beta1- and beta2-ARs increased both 12 h and 5 days after the exposure. Tritium 5-7 hemoglobin, beta adult major chain Mus musculus 30-46 1705563-3 1991 Thyrotropin-releasing hormone (TRH), tetradecanoylphorbol acetate (TPA), and epidermal growth factor (EGF), each known to enhance calcium entry in GH4 cells, decrease GH4 cell number and incorporation of [3H]-thymidine. Tritium 205-208 epidermal growth factor like 1 Rattus norvegicus 77-100 1705563-3 1991 Thyrotropin-releasing hormone (TRH), tetradecanoylphorbol acetate (TPA), and epidermal growth factor (EGF), each known to enhance calcium entry in GH4 cells, decrease GH4 cell number and incorporation of [3H]-thymidine. Tritium 205-208 epidermal growth factor like 1 Rattus norvegicus 102-105 2004034-1 1991 The specific binding of luteinizing hormone-releasing hormone (LH-RH) agonist in estradiol-dependent MCF-7 and estradiol-independent MDA-MB-231 human breast cancer cells has been studied using [3H]Ovurelin [(D-3H-Phe6),des-Gly10-LH-RH- ethylamide]. Tritium 194-196 gonadotropin releasing hormone 1 Homo sapiens 24-61 16846720-2 2006 Compounds were screened for Pgp interaction based on inhibition of Pgp mediated [3H]-taxol transport in Caco-2 cells. Tritium 81-83 phosphoglycolate phosphatase Homo sapiens 28-31 16846720-2 2006 Compounds were screened for Pgp interaction based on inhibition of Pgp mediated [3H]-taxol transport in Caco-2 cells. Tritium 81-83 phosphoglycolate phosphatase Homo sapiens 67-70 16846720-10 2006 In conclusion, we have demonstrated that a substantial number of antiparasitic and natural compounds, in a range of concentrations, are capable of inhibiting Pgp mediated [3H]-taxol efflux in Caco-2 cells, without being substrates and this may have implications for drug interactions with Pgp. Tritium 172-174 phosphoglycolate phosphatase Homo sapiens 158-161 20652203-8 2010 A columnar crystal structure is inherent for PdL(1).3H(2)O with the water molecules present as discrete (H(2)O)(3) clusters. Tritium 52-54 CD274 molecule Homo sapiens 45-50 20678474-7 2010 MMP-2 gene expression as well as enzymatic activities of MMP-2 and MMP-9 were increased by IL-1beta (P<0.05 vs. control; 100ng/ml; 3h for gene expression, 48 and 72h for enzymatic activities of MMP-2 and MMP-9, respectively). Tritium 134-136 matrix metallopeptidase 2 Sus scrofa 0-5 2004034-1 1991 The specific binding of luteinizing hormone-releasing hormone (LH-RH) agonist in estradiol-dependent MCF-7 and estradiol-independent MDA-MB-231 human breast cancer cells has been studied using [3H]Ovurelin [(D-3H-Phe6),des-Gly10-LH-RH- ethylamide]. Tritium 194-196 gonadotropin releasing hormone 1 Homo sapiens 63-68 20678474-7 2010 MMP-2 gene expression as well as enzymatic activities of MMP-2 and MMP-9 were increased by IL-1beta (P<0.05 vs. control; 100ng/ml; 3h for gene expression, 48 and 72h for enzymatic activities of MMP-2 and MMP-9, respectively). Tritium 134-136 matrix metallopeptidase 2 Sus scrofa 57-62 20678474-7 2010 MMP-2 gene expression as well as enzymatic activities of MMP-2 and MMP-9 were increased by IL-1beta (P<0.05 vs. control; 100ng/ml; 3h for gene expression, 48 and 72h for enzymatic activities of MMP-2 and MMP-9, respectively). Tritium 134-136 matrix metallopeptidase 2 Sus scrofa 57-62 20478302-5 2010 [3H]methotrexate uptake into S2-hOAT3 cells was significantly inhibited by celecoxib in a concentration-dependent manner and the Ki value was 35.3 microM. Tritium 1-3 solute carrier family 22 member 8 Homo sapiens 32-37 16846720-10 2006 In conclusion, we have demonstrated that a substantial number of antiparasitic and natural compounds, in a range of concentrations, are capable of inhibiting Pgp mediated [3H]-taxol efflux in Caco-2 cells, without being substrates and this may have implications for drug interactions with Pgp. Tritium 172-174 phosphoglycolate phosphatase Homo sapiens 289-292 16714376-2 2006 In this study, we have examined the contribution of OATP-B to the uptake of [3H]estrone-3-sulfate in Caco-2 cells in comparison with those of its homologs OATP-D (OATP3A1) and OATP-E (OATP4A1). Tritium 77-79 solute carrier organic anion transporter family member 2B1 Homo sapiens 52-58 16715122-0 2006 Detection of multiple H3 receptor affinity states utilizing [3H]A-349821, a novel, selective, non-imidazole histamine H3 receptor inverse agonist radioligand. Tritium 61-63 histamine receptor H3 Homo sapiens 108-129 20346999-7 2010 Additionally, bolus (3h) doxorubicin incubation led to phosphorylation of p53 at serine 392, induction of p21, G2 arrest and increase of proapoptotic protein Bax. Tritium 21-23 H3 histone pseudogene 16 Homo sapiens 106-109 1829232-8 1991 Quantitative autoradiographic studies 5 weeks after 5,7-DHT treatment revealed a significant increase in radioligand binding to 5-HT1A, 5-HT1B and 5-HT1C sites in many brain regions studied, except for the raphe nuclei where [3H]8-OH-DPAT binding to 5-HT1A sites was markedly reduced. Tritium 226-228 5-hydroxytryptamine receptor 1A Rattus norvegicus 128-134 19890975-6 2010 [3H]L-leucine efflux from ARPE-19 cells was trans-stimulated by substrates of LAT1 and LAT2 through the obligatory exchange mechanism of system L. Although RT-PCR analysis demonstrated that LAT1 and LAT2 mRNA are expressed in ARPE-19 cells, the LAT1 mRNA concentration is 42-fold higher than that of LAT2. Tritium 1-3 solute carrier family 7 member 5 Homo sapiens 78-82 19890975-6 2010 [3H]L-leucine efflux from ARPE-19 cells was trans-stimulated by substrates of LAT1 and LAT2 through the obligatory exchange mechanism of system L. Although RT-PCR analysis demonstrated that LAT1 and LAT2 mRNA are expressed in ARPE-19 cells, the LAT1 mRNA concentration is 42-fold higher than that of LAT2. Tritium 1-3 solute carrier family 7 member 5 Homo sapiens 190-194 19890975-6 2010 [3H]L-leucine efflux from ARPE-19 cells was trans-stimulated by substrates of LAT1 and LAT2 through the obligatory exchange mechanism of system L. Although RT-PCR analysis demonstrated that LAT1 and LAT2 mRNA are expressed in ARPE-19 cells, the LAT1 mRNA concentration is 42-fold higher than that of LAT2. Tritium 1-3 solute carrier family 7 member 5 Homo sapiens 190-194 16723377-3 2006 Thrombin, histamine, and U46619 all enhanced EGF-stimulated [3H]-thymidine incorporation as well as late-phase Akt and p70S6K phosphorylation in ASM cultures. Tritium 61-63 epidermal growth factor Homo sapiens 45-48 1828235-2 1991 [3H]SCH 23390, used as a ligand of DA1 receptors, was bound by sections of rat cerebral vessels in a manner consistent with the labeling of DA1 receptors, with a value of 2.2 nM for the dissociation constant (Kd) and of 68.4 fmol/mg protein for the density of binding sites (Bmax). Tritium 1-3 RT1 class II, locus Da Rattus norvegicus 35-38 16439081-6 2006 Both extracts showed significant affinities to the mu-opiate receptor, as indicated by the concentration-dependent displacement of [3H]DAMGO binding in Chinese hamster ovary (CHO)-human mu-opiate receptor (hMOR) cells. Tritium 132-134 opioid receptor mu 1 Homo sapiens 51-69 16439081-6 2006 Both extracts showed significant affinities to the mu-opiate receptor, as indicated by the concentration-dependent displacement of [3H]DAMGO binding in Chinese hamster ovary (CHO)-human mu-opiate receptor (hMOR) cells. Tritium 132-134 opioid receptor mu 1 Homo sapiens 186-204 16793548-3 2006 In the present study, we showed that IRBIT suppresses the activation of IP3R by competing with IP3 by [3H]IP3 binding assays, in vitro Ca2+ release assays, and Ca2+ imaging of intact cells. Tritium 103-105 adenosylhomocysteinase like 1 Homo sapiens 37-42 20171721-3 2010 Treatment with BDNF enhanced phosphorylation of PKB in oocytes at 2h (P=0.0006) and 3h (P<0.0001) of in vitro maturation, compared with control oocytes. Tritium 84-86 brain derived neurotrophic factor Mus musculus 15-19 1828235-2 1991 [3H]SCH 23390, used as a ligand of DA1 receptors, was bound by sections of rat cerebral vessels in a manner consistent with the labeling of DA1 receptors, with a value of 2.2 nM for the dissociation constant (Kd) and of 68.4 fmol/mg protein for the density of binding sites (Bmax). Tritium 1-3 RT1 class II, locus Da Rattus norvegicus 140-143 19913081-8 2010 Only Uncaria hook (3.13-50 microg/ml), of the seven constituent herbal extracts, inhibited the [(3)H]8-OH-DPAT binding to 5-HT1A receptors in a concentration-dependent manner, and the IC(50) value was estimated to be 7.42 microg/ml. Tritium 96-100 5-hydroxytryptamine receptor 1A Homo sapiens 122-128 1785775-2 1991 Human SBP was purified from late-pregnancy serum and labeled either with colloidal gold particles (18 nm) or with [3H]delta 6-testosterone by photoaffinity treatment. Tritium 115-117 selenium binding protein 1 Homo sapiens 6-9 16278311-4 2006 As a result, wild-type (WT) brains accumulate tritium in a regionally specific manner that is dependent upon regional FAAH activity, whereas brains from FAAH knockout (KO) mice show a uniform [3H]anandamide distribution. Tritium 193-195 fatty acid amide hydrolase Mus musculus 153-157 19577658-5 2009 Gene expression array analysis demonstrated a 2-3-fold increase in PKCepsilon expression in the bystander human primary fibroblast cells that were co-cultured in double-sided Mylar dishes for 3h with human primary fibroblast cells irradiated with 5Gy of alpha-particles. Tritium 192-194 protein kinase C epsilon Homo sapiens 67-77 1670924-4 1991 These morphological changes were accompanied by the functional expression of D2 dopamine receptors as shown by the presence of a specific, saturable, and stereoselective high affinity binding for [3H]spiroperidol in epidermal growth factor-treated cells and by the fact that the selective D2 agonist quinpirole recovered the property to inhibit PRL secretion in the cell cultures exposed to the neurotrophic factor. Tritium 197-199 epidermal growth factor like 1 Rattus norvegicus 216-239 19722701-5 2009 P-gp-mediated transport of [3H]-TPP was assessed in Caco-2 cells and ex vivo in rat intestinal wall by the use of a diffusion cell system. Tritium 28-32 phosphoglycolate phosphatase Homo sapiens 0-4 16571921-4 2006 [3H]-Thymidine and BrdU incorporation also indicated that both hsp25 and inducible hsp70 were involved in G1 arrest after 1 cGy irradiation. Tritium 1-3 heat shock protein family B (small) member 1 Homo sapiens 63-68 21186585-5 2006 RESULTS: The level of tyrosine-phosphorylated STAT3 increased at 6 h and peaked at 12 h. The expression of c-myc mRNA increased after 2 h of hypoxia and reached maximal level at 4 h, then declined at 6 h and to the basal levels at 12 h. With the prolonging of hypoxia time, 3H-TdR incorporation in PASMC under hypoxia conditions was significantly higher. Tritium 274-276 signal transducer and activator of transcription 3 Rattus norvegicus 46-51 16107508-10 2006 Inhibition of p44/42 MAPKs with PD-98059 (MEK inhibitor) attenuated EGF-induced increase of [3H]thymidine incorporation. Tritium 93-95 epidermal growth factor Mus musculus 68-71 16141367-6 2005 [3H]Ranitidine uptake was stimulated by hOCT1 (Km= 70 +/- 9 microM) and to a much smaller extent by hOCT2. Tritium 1-3 solute carrier family 22 member 2 Homo sapiens 100-105 19789505-3 2009 MATERIAL/METHODS: We investigated MAO labeling with mechanism-based inhibitor [3H]pargyline activities of MAO A, MAO B, and SSAO in healthy and inflamed human dental pulp. Tritium 79-81 monoamine oxidase A Homo sapiens 106-111 19789505-3 2009 MATERIAL/METHODS: We investigated MAO labeling with mechanism-based inhibitor [3H]pargyline activities of MAO A, MAO B, and SSAO in healthy and inflamed human dental pulp. Tritium 79-81 amine oxidase copper containing 2 Homo sapiens 124-128 1997123-1 1991 The [3H]corticosterone-transcortin complexes from kidney cytosol show elution positions on DEAE-cellulose identical to serum transcortin. Tritium 5-7 serpin family A member 6 Rattus norvegicus 23-34 19101971-1 2009 This study reports the synthesis and pharmacological characterization of tritium-labeled N(1)-[3-(1H-imidazol-4-yl)propyl]-N(2)-propionylguanidine ([(3)H]UR-PI294), a novel and readily accessible radioligand for the human histamine H(3) receptor (hH(3)R) and H(4) receptor (hH(4)R). Tritium 73-80 histamine receptor H3 Homo sapiens 222-245 19101971-1 2009 This study reports the synthesis and pharmacological characterization of tritium-labeled N(1)-[3-(1H-imidazol-4-yl)propyl]-N(2)-propionylguanidine ([(3)H]UR-PI294), a novel and readily accessible radioligand for the human histamine H(3) receptor (hH(3)R) and H(4) receptor (hH(4)R). Tritium 73-80 histamine receptor H3 Homo sapiens 247-253 19101971-1 2009 This study reports the synthesis and pharmacological characterization of tritium-labeled N(1)-[3-(1H-imidazol-4-yl)propyl]-N(2)-propionylguanidine ([(3)H]UR-PI294), a novel and readily accessible radioligand for the human histamine H(3) receptor (hH(3)R) and H(4) receptor (hH(4)R). Tritium 73-80 histamine receptor H4 Homo sapiens 259-280 16422106-2 2005 The circadian rhythms of 3H-TdR incorporation and expression of c-myc gene in untreated and treated Eca-109 cells were measured by 3H-thymidine uptake assay and flow cytometry. Tritium 131-133 MYC proto-oncogene, bHLH transcription factor Homo sapiens 64-69 16207632-3 2005 In the [3H]BaP-DNA binding assay, an increase of BaP-DNA adduct formation was observed with 17beta-estradiol (E2)-induced ERalpha. Tritium 8-10 prohibitin 2 Homo sapiens 11-14 1768424-3 1991 We show that [3H]CD-270 carboxylic acid binds specifically to CRABP, whereas the alcohol derivative binds strongly to CRBP. Tritium 14-16 cellular retinoic acid binding protein 1 Homo sapiens 62-67 16207632-3 2005 In the [3H]BaP-DNA binding assay, an increase of BaP-DNA adduct formation was observed with 17beta-estradiol (E2)-induced ERalpha. Tritium 8-10 prohibitin 2 Homo sapiens 49-52 16207632-4 2005 Treatments of [3H]BaP in conjunction with the E2 induced a 2.1-fold increase in BaP-DNA adduct over BaP alone in the ER-positive MCF-7 cell line. Tritium 15-17 prohibitin 2 Homo sapiens 18-21 16207632-4 2005 Treatments of [3H]BaP in conjunction with the E2 induced a 2.1-fold increase in BaP-DNA adduct over BaP alone in the ER-positive MCF-7 cell line. Tritium 15-17 prohibitin 2 Homo sapiens 80-83 20067883-9 2009 Only the concentration of 100 muM of docetaxel inhibited incorporation [3H] thymidine into CRL-11731 cancer cells. Tritium 72-74 interleukin 31 receptor A Homo sapiens 91-94 1768424-8 1991 Moreover, when using these derivatives as ligands 2.4 times more CRABP and 3.9 times more CRBP could be measured with the PAGE technique as compared when using the natural [3H]ligands. Tritium 173-175 cellular retinoic acid binding protein 1 Homo sapiens 65-76 16207632-4 2005 Treatments of [3H]BaP in conjunction with the E2 induced a 2.1-fold increase in BaP-DNA adduct over BaP alone in the ER-positive MCF-7 cell line. Tritium 15-17 prohibitin 2 Homo sapiens 80-83 1768424-8 1991 Moreover, when using these derivatives as ligands 2.4 times more CRABP and 3.9 times more CRBP could be measured with the PAGE technique as compared when using the natural [3H]ligands. Tritium 173-175 retinol binding protein 1 Homo sapiens 90-94 2243353-1 1990 The 5-HT2 (serotonin) receptor has traditionally been labeled with antagonist radioligands such as [3H]ketanserin and [3H]spiperone, which label both agonist high-affinity (guanyl nucleotide-sensitive) and agonist low-affinity (guanyl nucleotide-insensitive) states of this receptor. Tritium 99-102 5-hydroxytryptamine receptor 2A Rattus norvegicus 4-30 18850315-5 2008 The progesterone-inhibited [3H]thymidine incorporation was completely blocked when the expressions of p21 and p27 were knocked-down together. Tritium 28-30 H3 histone pseudogene 16 Homo sapiens 102-105 18850315-5 2008 The progesterone-inhibited [3H]thymidine incorporation was completely blocked when the expressions of p21 and p27 were knocked-down together. Tritium 28-30 interferon alpha inducible protein 27 Homo sapiens 110-113 2272361-5 1990 Competition with eltoprazine for [3H]ligand binding to the various 5-HT1 receptor subtypes revealed preferential binding to 5-HT1A (IC50 values ranging from 42 to 50 nM) and 5-HT1B (IC50 values ranging from 25 to 38 nM) recognition sites. Tritium 34-36 5-hydroxytryptamine receptor 1A Rattus norvegicus 124-130 18583416-4 2008 Under basal conditions, caveolin-1-deficient mouse embryonic fibroblast cells [MF(-/-)] incorporated significantly more [3H]thymidine than wild-type mouse embryonic fibroblast cells [MF(+/+)]; however, small hairpin RNA-mediated knockdown of caveolin-1 (80% reduction) in 3T3L1 fibroblasts had no effect on basal proliferation. Tritium 121-123 caveolin 1, caveolae protein Mus musculus 24-34 2119296-1 1990 The insulin receptor was immunoprecipitated from cultured human lymphocytes (IM-9) and rat hepatocytes (Fao) after biosynthetic labeling with [3H]glucosamine or [3H]mannose, and the nature of the carbohydrate units was investigated. Tritium 143-145 insulin receptor Homo sapiens 4-20 18428029-2 2008 TGF-alpha increased [3H] thymidine and BrdU incorporation in a time- (0-72 h) and dose-dependent (0-10 ng/ml) manner. Tritium 21-23 transforming growth factor alpha Mus musculus 0-9 2119296-1 1990 The insulin receptor was immunoprecipitated from cultured human lymphocytes (IM-9) and rat hepatocytes (Fao) after biosynthetic labeling with [3H]glucosamine or [3H]mannose, and the nature of the carbohydrate units was investigated. Tritium 162-164 insulin receptor Homo sapiens 4-20 2212861-4 1990 Three isoforms (CTAP-III des 1-13, des 1-14, and des 1-15/NAP-2) stimulate [14C]glycosaminoglycan synthesis; two isoforms also promote [3H]DNA synthesis in human fibroblast cultures. Tritium 136-138 pro-platelet basic protein Homo sapiens 16-24 17675218-7 2008 The patients with cardiovascular disease showed a reduction (-50.40%) (p<0.01) of the 3H-8OH-DPAT bound to the platelet membranes 5-HT1A receptors (1.652+/-0.79 fmol/mg protein) with respect to the control group (3.331+/-0.16 fmol/mg protein). Tritium 89-92 5-hydroxytryptamine receptor 1A Homo sapiens 133-139 2205108-5 1990 During conversion of alanine to lactate with glutamic-pyruvic transaminase (GPT) and lactate dehydrogenase (LDH) in vitro, 32 +/- 2% of 3H in [3-3H]alanine was found in water and 68 +/- 2% in lactate. Tritium 136-138 glutamic--pyruvic transaminase Homo sapiens 45-74 17993726-5 2007 MAIN OUTCOME MEASURES: Cell proliferation was measured in the pancreatic beta-cell line INS-1 using 3H-thymidine incorporation. Tritium 100-102 insulin 1 Rattus norvegicus 88-93 2205108-5 1990 During conversion of alanine to lactate with glutamic-pyruvic transaminase (GPT) and lactate dehydrogenase (LDH) in vitro, 32 +/- 2% of 3H in [3-3H]alanine was found in water and 68 +/- 2% in lactate. Tritium 136-138 glutamic--pyruvic transaminase Homo sapiens 76-79 2205108-7 1990 In conclusion 1) loss of 3H relative to 14C from position 6 in glucose occurs during lactate formation in extrahepatic tissues possibly due to the GPT reaction (alanine conversion to pyruvate), and 2) even under supraphysiologic hyperinsulinemic conditions not all of plasma lactate originates from plasma glucose. Tritium 25-27 glutamic--pyruvic transaminase Homo sapiens 147-150 2204684-6 1990 Combined immunostaining and autoradiography of cultures derived from HVcs exposed to 3H-thymidine in vivo revealed a population of newly generated, MAP-2/3H-thymidine-positive neurons. Tritium 85-87 microtubule-associated protein 2 Serinus canaria 148-153 2386801-7 1990 Only a small fraction (less than 10% after 24 h) of the free [3H]cholesterol generated in the lysosomes was esterified by ACAT resulting in a doubling of the cell free cholesterol content. Tritium 62-64 acetyl-Coenzyme A acetyltransferase 1 Mus musculus 122-126 2386801-9 1990 ACAT was active as endogenous free [14C]cholesterol was esterified in a linear manner over 24 h and was responsive to the presence of lysosomally-derived cholesterol, as the extent of esterification of the endogenous pool was directly proportional to the mass of [3H]cholesterol generated in the lysosomes. Tritium 264-266 acetyl-Coenzyme A acetyltransferase 1 Mus musculus 0-4 1977590-1 1990 Clozapine, an atypical neuroleptic drug devoid of extrapyramidal side effects, was a moderately potent, competitive inhibitor of the binding of [3H]quaternised ICS 205-930 to 5-HT3 receptor sites in rat cortical membranes, possessing a pKi value of 7.0. Tritium 145-147 5-hydroxytryptamine receptor 3A Rattus norvegicus 175-189 2375770-10 1990 In contrast, crude rat brain PKC, which had been proteolysed to generate a PS and Ca2+ independent enzyme (PK-M) retained the ability to bind [3H]DMS, but not [3H]PDBu. Tritium 143-145 pyruvate kinase M1/2 Rattus norvegicus 107-111 2173350-3 1990 In the membrane suspensions, the highest level of specific [3H]PGE2 binding was detected in the outer medulla (Bmax = 335 +/- 28 fmol mg-1 protein) followed by the inner medulla (Bmax = 258 +/- 21 fmol mg-1 protein) and the cortex (Bmax = 143 +/- 22 fmol mg-1 protein). Tritium 60-62 mucin 5B, oligomeric mucus/gel-forming Homo sapiens 134-138 2173350-3 1990 In the membrane suspensions, the highest level of specific [3H]PGE2 binding was detected in the outer medulla (Bmax = 335 +/- 28 fmol mg-1 protein) followed by the inner medulla (Bmax = 258 +/- 21 fmol mg-1 protein) and the cortex (Bmax = 143 +/- 22 fmol mg-1 protein). Tritium 60-62 mucin 5B, oligomeric mucus/gel-forming Homo sapiens 202-206 2173350-3 1990 In the membrane suspensions, the highest level of specific [3H]PGE2 binding was detected in the outer medulla (Bmax = 335 +/- 28 fmol mg-1 protein) followed by the inner medulla (Bmax = 258 +/- 21 fmol mg-1 protein) and the cortex (Bmax = 143 +/- 22 fmol mg-1 protein). Tritium 60-62 mucin 5B, oligomeric mucus/gel-forming Homo sapiens 202-206 2393908-2 1990 Lectin differently affects 3H and 35SO4 incorporation. Tritium 27-29 galectin 3 Gallus gallus 0-6 1972921-0 1990 Evidence for direct action of estradiol on growth hormone-releasing factor (GRF) in rat hypothalamus: localization of [3H]estradiol in GRF neurons. Tritium 119-121 growth hormone releasing hormone Rattus norvegicus 135-138 2116496-5 1990 [3H]3-methyl-histidine2-TRH [( 3H]Me-TRH) was located in the pituitary gland and peripheral plasma within 5 min of its i.c.v. Tritium 1-3 thyrotropin releasing hormone Gallus gallus 24-27 2116496-5 1990 [3H]3-methyl-histidine2-TRH [( 3H]Me-TRH) was located in the pituitary gland and peripheral plasma within 5 min of its i.c.v. Tritium 1-3 thyrotropin releasing hormone Gallus gallus 37-40 2116496-7 1990 [3H]Me-TRH rapidly accumulated in the hypothalamus following its i.c.v. Tritium 1-3 thyrotropin releasing hormone Gallus gallus 7-10 1698505-10 1990 Quantitative autoradiographic analysis of the density of 5-HT1A receptors labeled with [3H]8-OH-DPAT revealed that these sites were unchanged in regions of the brain (frontal cortex, hippocampus and brain stem) and in the spinal cord. Tritium 88-90 5-hydroxytryptamine receptor 1A Rattus norvegicus 57-63 16081670-5 2005 Many of the tested flavonoids (including biochanin A, genistein, and epigallocatechin-3-gallate) significantly inhibited [3H]DHEAS uptake in a concentration-dependent manner in OATP1B1-expressing cells, with biochanin A being one of the most potent inhibitors with an IC50 of 11.3 +/- 3.22 microM. Tritium 122-124 solute carrier organic anion transporter family member 1B1 Homo sapiens 177-184 2200435-2 1990 3H-SCH 23390 (DA-1 ligand) was bound by the medial layer of cerebral, mesenteric and renal arteries without different density in large, medium and small sized arteries. Tritium 0-2 RT1 class II, locus Da Rattus norvegicus 14-18 16216068-6 2005 [3H]APFBzcholine photolabeled gammaLeu-109/deltaLeu-111, gammaTyr-111, and gammaTyr-117 in binding site segment E as well as alphaTyr-198 in alpha subunit binding site segment C. The observed pattern of photolabeling is examined in relation to the predicted orientation of the azide when APFBzcholine is docked in the agonist binding site of a homology model of the nAChR extracellular domain based upon the structure of the snail acetylcholine binding protein. Tritium 1-3 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 366-371 2162851-4 1990 In contrast, IGF-I increased calcium entry, radioactivity in [3H]diacylglycerol, and [3H]thymidine incorporation in primed competent cells while these reactions were not induced by IGF-I in quiescent cells. Tritium 62-64 insulin-like growth factor 1 Mus musculus 13-18 16092934-8 2005 [3H]Nisoxetine binding affinities were decreased 13-fold for hP97C and 5-fold for hF101C. Tritium 1-4 melanotransferrin Homo sapiens 61-65 2163861-3 1990 Apparent Ki values at high affinity TRH-binding sites, labelled predominantly by [3H][3-Me-His2]TRH, ranged from 17.0 to 36.9 microM in all tested regions. Tritium 82-84 thyrotropin releasing hormone Rattus norvegicus 36-39 15948180-3 2005 In control rats, [3H]nociceptin and nociceptin-stimulated [35S]GTPgammaS bindings were found to be correlated in several brain regions. Tritium 18-20 prepronociceptin Rattus norvegicus 21-31 2163861-4 1990 Additionally, [Nva2]TRH was shown to compete with [3H]TRH at low affinity TRH-binding sites with similar affinities. Tritium 51-53 thyrotropin releasing hormone Rattus norvegicus 20-23 15948180-3 2005 In control rats, [3H]nociceptin and nociceptin-stimulated [35S]GTPgammaS bindings were found to be correlated in several brain regions. Tritium 18-20 prepronociceptin Rattus norvegicus 36-46 2163861-4 1990 Additionally, [Nva2]TRH was shown to compete with [3H]TRH at low affinity TRH-binding sites with similar affinities. Tritium 51-53 thyrotropin releasing hormone Rattus norvegicus 54-57 15948180-6 2005 In dorsal raphe nucleus, [3H]nociceptin binding was decreased to the same extent as [3H]citalopram binding, whereas it was unchanged in the other regions studied. Tritium 26-28 prepronociceptin Rattus norvegicus 29-39 2163861-4 1990 Additionally, [Nva2]TRH was shown to compete with [3H]TRH at low affinity TRH-binding sites with similar affinities. Tritium 51-53 thyrotropin releasing hormone Rattus norvegicus 54-57 2163861-6 1990 Its analeptic activity, however, may be mediated by low affinity TRH binding sites which are predominantly labelled by [3H]TRH or by yet unidentified mechanisms. Tritium 120-122 thyrotropin releasing hormone Rattus norvegicus 65-68 15824923-6 2005 The dose-dependent increase in [3H]-VBL uptake (IC50 26.5 microM) induced by cnidiadin in the dose range 1-100 microM correlated with inhibition of Pgp photolabeling. Tritium 32-34 PGP Canis lupus familiaris 148-151 16000584-3 2005 RESULTS: We showed that five LPAAT-beta inhibitors at doses of 500 nmol/L routinely inhibited growth in a panel of human lymphoma cell lines in vitro by >90%, as measured by [3H]thymidine incorporation. Tritium 178-180 1-acylglycerol-3-phosphate O-acyltransferase 2 Homo sapiens 29-39 2163861-6 1990 Its analeptic activity, however, may be mediated by low affinity TRH binding sites which are predominantly labelled by [3H]TRH or by yet unidentified mechanisms. Tritium 120-122 thyrotropin releasing hormone Rattus norvegicus 123-126 1692840-7 1990 In addition, EDC1 treatment of Raji cells prelabeled with 3H-labeled DNA did not release more radioactivity into the conditioned medium than the untreated labeled cells. Tritium 58-60 alpha-1-microglobulin/bikunin precursor Homo sapiens 13-17 15980244-8 2005 [3H]L-Leucine efflux from TR-iBRB2 cells was trans-stimulated by substrates of LAT1. Tritium 1-3 solute carrier family 7 member 5 Homo sapiens 79-83 1969352-0 1990 Evidence for a relationship between B-50 (GAP-43) and [3H]noradrenaline release in rat brain synaptosomes. Tritium 55-57 growth associated protein 43 Rattus norvegicus 42-48 15854583-4 2005 These results suggest that some [3H]isatin binding in the brain may be to NPR-A and NPR-C. Tritium 33-35 natriuretic peptide receptor 1 Rattus norvegicus 74-79 15854583-4 2005 These results suggest that some [3H]isatin binding in the brain may be to NPR-A and NPR-C. Tritium 33-35 natriuretic peptide receptor 3 Rattus norvegicus 84-89 2314388-0 1990 Characterization of the binding of [3H]Ro 41-1049 to the active site of human monoamine oxidase-A. Tritium 36-38 monoamine oxidase A Homo sapiens 78-97 15722356-9 2005 The dramatic reduction in [3H]PGE2 formation from mPGES-1-/- macrophages compared with controls resulted in TxB2 and 6-keto PGF1alpha becoming the two most abundant prostaglandins in these samples. Tritium 27-29 prostaglandin E synthase Mus musculus 50-57 15862800-4 2005 Both the canine 5-HT2A and 5-HT2B receptors have high affinity for [3H]5-HT (KD=4.50+/-0.89 nM and 3.10+/-0.82 nM, respectively) and, in general, the pharmacology of these two receptors matches closely the pharmacology of their human homologs for the 19 serotonergic ligands tested. Tritium 68-70 5-hydroxytryptamine receptor 2A Canis lupus familiaris 16-22 15695516-5 2005 Pharmacological inhibitors of the GTP-binding proteins G(i)/G(o), phospholipase C, Src, and mitogenic-extracellular signal-regulated kinase 1/2 (MEK 1/2) decreased oleate-induced [3H]thymidine incorporation in the breast cancer cell line MDA-MB-231. Tritium 180-182 mitogen-activated protein kinase kinase 1 Homo sapiens 145-152 15368538-5 2005 EGF similarly activates phosphorylation of p44/42 MAPK and stimulates [3H]-AA release. Tritium 71-73 epidermal growth factor Homo sapiens 0-3 15368538-6 2005 When PTCs were exposed to 100 microM H2O2 and 50 ng/ml EGF simultaneously, a further increase in the phosphorylation of p44/42 MAPK, of [3H]-AA release, and of prostaglandin E2 (PGE2) production was elicited as compared with the effects of each individual agonist alone. Tritium 137-139 epidermal growth factor Homo sapiens 55-58 15368538-7 2005 Moreover, the additive phosphorylation of p44/42 MAPK, [3H]-AA release, and PGE2 production by H2O2 and EGF was almost completely inhibited by the p44/42 MAPK inhibitor, PD 98059. Tritium 56-58 epidermal growth factor Homo sapiens 95-107 15678090-0 2005 Addition of a signal peptide sequence to the alpha1D-adrenoceptor gene increases the density of receptors, as determined by [3H]-prazosin binding in the membranes. Tritium 125-127 adrenoceptor alpha 1D Homo sapiens 45-65 15678090-15 2005 This indicates that the low expression level of [3H]-prazosin binding sites, after transfection with the wild-type alpha1D-adrenoceptor, is not caused by constitutive activity of the receptor and subsequent receptor downregulation. Tritium 49-51 adrenoceptor alpha 1D Homo sapiens 115-135 15680395-4 2005 Cells were treated with 10 nM CypA for 24 h. The cell proliferation was determined by [3H]thymidine incorporation. Tritium 87-89 peptidylprolyl isomerase A Homo sapiens 30-34 15690320-5 2005 Specific binding of [3H]nicotine was demonstrated in rat islets and in a beta -cell line of rat origin, INS-1. Tritium 21-23 insulin 1 Rattus norvegicus 104-109 15381114-2 2004 Galphai2 protein expression was significantly reduced 0 and 3h after exercise but increased 24h after exercise, without alterations in Galphai2 mRNA expressions. Tritium 60-62 G protein subunit alpha i2 Rattus norvegicus 0-8 15381114-4 2004 Both the 26S proteasome activity and polyubiquitination of Galphai2 protein were significantly increased 0 and 3h after exercise. Tritium 111-113 G protein subunit alpha i2 Rattus norvegicus 59-67 15178549-10 2004 IGFBP-3 also caused IGF-I-independent inhibition of basal [3H]thymidine incorporation. Tritium 59-61 insulin like growth factor binding protein 3 Homo sapiens 0-7 15178549-12 2004 Immunoneutralization of IGFBP-3 increased basal [3H]thymidine incorporation, implying that endogenous IGFBP-3 inhibits proliferation. Tritium 49-51 insulin like growth factor binding protein 3 Homo sapiens 24-31 15178549-12 2004 Immunoneutralization of IGFBP-3 increased basal [3H]thymidine incorporation, implying that endogenous IGFBP-3 inhibits proliferation. Tritium 49-51 insulin like growth factor binding protein 3 Homo sapiens 102-109 15493879-2 2004 When sequestration of the B2 bradykinin receptor (B2R) was inhibited by either 0.5 M sucrose or phenylarsine oxide (PAO), a shift in the affinity was detected when the incubation temperature was raised from 4 degrees C to 37 degrees C or lowered from 37 degrees C to 4 degrees C. In contrast, binding of the antagonist [3H]NPC 17731 was temperature-independent. Tritium 320-322 bradykinin receptor B2 Homo sapiens 26-48 15493879-2 2004 When sequestration of the B2 bradykinin receptor (B2R) was inhibited by either 0.5 M sucrose or phenylarsine oxide (PAO), a shift in the affinity was detected when the incubation temperature was raised from 4 degrees C to 37 degrees C or lowered from 37 degrees C to 4 degrees C. In contrast, binding of the antagonist [3H]NPC 17731 was temperature-independent. Tritium 320-322 bradykinin receptor B2 Homo sapiens 50-53 15571037-4 2004 By inhibition of poly(ADP-ribose) polymerase (PARP) with 3-aminobenzamidine and treatment of ADP-ribosylated proteins with phosphodiesterase I, it was demonstrated that about 90% of [3H]-adenin bound by proteins in the nuclei and 70% in the mitochondria was the result of PARP activity. Tritium 183-185 poly (ADP-ribose) polymerase 1 Rattus norvegicus 46-50 15571037-4 2004 By inhibition of poly(ADP-ribose) polymerase (PARP) with 3-aminobenzamidine and treatment of ADP-ribosylated proteins with phosphodiesterase I, it was demonstrated that about 90% of [3H]-adenin bound by proteins in the nuclei and 70% in the mitochondria was the result of PARP activity. Tritium 183-185 poly (ADP-ribose) polymerase 1 Rattus norvegicus 272-276 15272206-0 2004 Validation of a [3H]astemizole binding assay in HEK293 cells expressing HERG K+ channels. Tritium 17-19 potassium voltage-gated channel subfamily H member 2 Homo sapiens 72-76 15234345-6 2004 Functionally, coexpression of GAT1 and Pals1 in COS-7 cells increases [3H]-GABA uptake by GAT1. Tritium 71-73 protein associated with LIN7 1, MAGUK family member Mus musculus 39-44 15130767-8 2004 Pretreatment of HDMVECs with a p21 antisense oligonucleotide, which blocked the expression of p21 protein, reversed the BJ-601-induced inhibition of [3H]thymidine incorporation into HDMVECs. Tritium 150-152 H3 histone pseudogene 16 Homo sapiens 31-34 15130767-8 2004 Pretreatment of HDMVECs with a p21 antisense oligonucleotide, which blocked the expression of p21 protein, reversed the BJ-601-induced inhibition of [3H]thymidine incorporation into HDMVECs. Tritium 150-152 H3 histone pseudogene 16 Homo sapiens 94-97 14966990-3 2004 Extension toward polymetallic magnetically noncoupled rhombic lanthanide complexes in [Ln2Lu(TACI-3H)2(H2O)6]3+ (two paramagnetic centers with Cs microsymmetry) and [Ln3(TACI-3H)2(H2O)6]3+ (three paramagnetic centers with C2v microsymmetry) requires only minor modifications of the original three-nuclei equation. Tritium 98-100 TNF receptor superfamily member 13B Homo sapiens 93-97 14645259-3 2004 We demonstrate that treatment of monocyte RAW 264.7 cells with RANKL induces dose-dependent growth inhibition (IC50 = 10 ng/ml) as determined by dye uptake and [3H]thymidine incorporation methods. Tritium 161-163 tumor necrosis factor (ligand) superfamily, member 11 Mus musculus 63-68 14660561-7 2004 One novel finding with each Homer agonist (H1c and H1-EVH1) was that in combination their actions on [3H]ryanodine binding was additive, an effect also observed for these Homer agonists in the Ca2+ spark studies. Tritium 102-104 H1.2 linker histone, cluster member Homo sapiens 43-46 15102475-11 2004 SCF-induced 3H-thymidine incorporation of FACS-purified Kit+ fetal liver deficient for JAK2 was impaired by approximately 50%, whereas colony formation in methylcellulose was reduced 95%. Tritium 12-14 kit ligand Mus musculus 0-3 15102475-11 2004 SCF-induced 3H-thymidine incorporation of FACS-purified Kit+ fetal liver deficient for JAK2 was impaired by approximately 50%, whereas colony formation in methylcellulose was reduced 95%. Tritium 12-14 Janus kinase 2 Mus musculus 87-91 14742685-2 2004 Quantification by protein staining and with a radioligand binding assay using the high-affinity P2Y1-R antagonist [3H]MRS2279 ([3H]2-chloro-N6-methyl-(N)-methanocarba-2"-deoxyadenosine 3",5"-bis-phosphate) indicated a nearly homogenous preparation of receptor protein. Tritium 115-117 purinergic receptor P2Y1 Homo sapiens 96-102 14742685-3 2004 Ki values determined in [3H]MRS2279 binding assays for antagonists with the purified P2Y1-R were in good agreement with the Ki and KB values determined for these molecules in membrane binding and activity assays, respectively. Tritium 25-27 purinergic receptor P2Y1 Homo sapiens 85-91 14691161-5 2004 RESULTS: Incubation of PECs and PFs with HB-EGF resulted in a significant increase in [3H]thymidine incorporation compared with that of control cells. Tritium 87-89 heparin binding EGF like growth factor Homo sapiens 41-47 14556235-5 2003 In CHO-K1 cells expressing 5-HT1A receptors, 5-HT and DA inhibited the specific binding of selective antagonist [3H]-8-OH-DPAT with IC50 values of 10.2 nM and 1.4 microM, and both 5-HT and DA inhibited the forskolin-induced accumulation of cAMP. Tritium 113-115 5-hydroxytryptamine receptor 1A Homo sapiens 27-33 12829440-8 2003 Furthermore, rhRANTES induced ERK phosphorylation, ERK-dependent [3H]thymidine incorporation, and HSC proliferation. Tritium 66-68 EPH receptor B2 Homo sapiens 51-54 14499349-3 2003 Cd2+ increased [3H]thymidine uptake and cell number twofold. Tritium 16-18 CD2 molecule Homo sapiens 0-3 12958153-3 2003 In cotransfected Ltk-, HEK 293, and SK-N-MC cells, alpha-synuclein induced a 35% decrease in [3H]DA uptake. Tritium 94-96 synuclein alpha Homo sapiens 51-66 14595755-12 2003 The level of IGF 2 peptide in the conditioned media strongly correlated with [3H]thymidine incorporation and cell proliferation. Tritium 78-80 insulin like growth factor 2 Homo sapiens 13-18 12937991-8 2003 SU5416 treatment also resulted in a dose-dependent prohibition of VEGF-induced p44/p42 MAPK phosphorylation, [3H]thymidine uptake and in vitro tube formation with corresponding concentrations that inhibited KDR phosphorylation. Tritium 110-112 vascular endothelial growth factor A Rattus norvegicus 66-70 12943799-10 2003 After 3h, BPI/LBP gene expression level was gradually become less in the stimulated times. Tritium 6-8 LBP (LPS binding protein)/BPI (bactericidal/permeability-increasing protein)-1 Oncorhynchus mykiss 14-17 12870960-2 2003 At pH = 8.0 in water, its deprotonated form [L13 - 3H](3-) reacts with Ln(ClO(4))(3) to give the poorly soluble and stable podates [Ln(L13 - 3H)] (log(beta(110)) = 6.7-7.0, Ln = La-Lu). Tritium 51-53 mitochondrial ribosomal protein L13 Homo sapiens 45-48 12870960-2 2003 At pH = 8.0 in water, its deprotonated form [L13 - 3H](3-) reacts with Ln(ClO(4))(3) to give the poorly soluble and stable podates [Ln(L13 - 3H)] (log(beta(110)) = 6.7-7.0, Ln = La-Lu). Tritium 51-53 mitochondrial ribosomal protein L13 Homo sapiens 135-138 12606305-4 2003 Amidated gastrin (G-17), one of the major circulating forms of gastrin, induced a concentration-dependent increase in [3H]thymidine incorporation of cells in culture, with the maximum effective concentration occurring with 20 nM G-17. Tritium 119-121 gastrin Homo sapiens 9-16 12606305-4 2003 Amidated gastrin (G-17), one of the major circulating forms of gastrin, induced a concentration-dependent increase in [3H]thymidine incorporation of cells in culture, with the maximum effective concentration occurring with 20 nM G-17. Tritium 119-121 gastrin Homo sapiens 63-70 12944143-8 2003 Displacement studies with native LDL studies showed that ECDI-LDL-[3H]-labeled plasmid DNA complexes retained the ability to recognize the LDL receptor on normal skin fibroblasts. Tritium 67-69 low density lipoprotein receptor Homo sapiens 139-151 12729599-2 2003 Diethylaminoethyl cellulose or glass fiber filters impregnated with polyethyleneimine were used to capture the [3H]AAG-Hsp90 complex, and conditions which washed >98% of free [3H]AAG from the filters were developed. Tritium 112-114 heat shock protein 90 alpha family class A member 1 Homo sapiens 119-124 12729599-5 2003 [3H]AAG had similar affinities for full-length hHsp90alpha and for hHsp90alpha9-236 variants containing biotinylated N-terminal biotinylation signal sequences and N- or C-terminal His(6) tags. Tritium 1-3 heat shock protein 90 alpha family class A member 1 Homo sapiens 47-58 12729599-6 2003 Geldanamycin, ADP, ATP, and radicicol-all known to bind to the ATP domain of Hsp90-competed with [3H]AAG for binding to hHsp90alpha9-236, showing K(d) values in good agreement with reported values. Tritium 98-100 heat shock protein 90 alpha family class A member 1 Homo sapiens 77-82 12721093-14 2003 Rauwolscine and phentolamine increased tritium overflow evoked by 120 pulses at 3 Hz up to seven-fold in WT and alpha(2B)KO tissues, three-fold in alpha(2A)KO and alpha(2C)KO tissues, and two-fold in alpha(2AC)KO tissues. Tritium 39-46 adrenergic receptor, alpha 2b Mus musculus 112-120 12833632-5 2003 Scatchard analysis of [3H]Ap3A binding data yielded dissociation constants of 0.3 microM for gelsolin and 4.1 microM for PAI-1. Tritium 23-25 gelsolin Homo sapiens 93-101 12542695-6 2003 Adenovirus-mediated overexpression of SR-BI in all three choriocarcinoma cell lines resulted in an enhanced capacity for cell association of [3H]CE-HDL3 (20-fold in BeWo; fivefold in JAr and Jeg3). Tritium 142-144 scavenger receptor class B member 2 Homo sapiens 38-43 12565696-2 2003 Incubation of synaptosomal membranes or brain sections with purified AChE caused a developmentally modulated enhancement in the binding of [3H]-(S)-AMPA and the specific AMPA receptor ligand [3H]-(S)-5-fluorowillardiine, but did not modify binding to kainate neither N-methyl-D-aspartate receptors. Tritium 140-142 acetylcholinesterase Rattus norvegicus 69-73 12565696-2 2003 Incubation of synaptosomal membranes or brain sections with purified AChE caused a developmentally modulated enhancement in the binding of [3H]-(S)-AMPA and the specific AMPA receptor ligand [3H]-(S)-5-fluorowillardiine, but did not modify binding to kainate neither N-methyl-D-aspartate receptors. Tritium 192-194 acetylcholinesterase Rattus norvegicus 69-73 12565696-4 2003 AChE-mediated enhancement of [3H]-fluorowillardiine binding was measurable in all major CNS areas, but displayed remarkable anatomical selectivity and developmental regulation. Tritium 30-32 acetylcholinesterase Rattus norvegicus 0-4 12527415-5 2003 To determine if these same residues affect the binding of the sialic acid moiety of GM2 ganglioside, an alphaArg(424)Gln form of Hex A was expressed and its kinetics analyzed using the GM2 activator protein:[3H]-GM2 ganglioside complex as a substrate. Tritium 208-210 hexosaminidase subunit alpha Homo sapiens 129-134 12621157-6 2003 In addition, AGE-BSA significantly inhibited the efflux of [3H] cholesterol from CHO-SR-BI cells to HDL. Tritium 60-62 scavenger receptor class B member 1 Cricetulus griseus 85-90 12693096-21 2002 The recombinant rat IGF-I can promote cell proliferation, shown by the elevation of 3H-TdR incorporation. Tritium 84-86 insulin-like growth factor 1 Rattus norvegicus 20-25 12386145-3 2002 Adenoviral gene transfer of TRX enhanced TRX enzyme activity 2.7+/-0.4-fold (P<0.05 versus cells infected with adenoviral vector expressing green fluorescent protein [AdGFP]) and resulted in a 3.8+/-0.5-fold increase of cellular DNA synthesis as detected by methyl-[3H]thymidine incorporation (P<0.001). Tritium 269-271 thioredoxin Homo sapiens 28-31 12405983-6 2002 To correlate these projection patterns with the neurochemical characteristics of RE neurons, the retrograde tracer [3H]D-aspartate, which is selectively taken up by high affinity uptake sites that use glutamate as neurotransmitter, and the nonselective retrograde tracer wheatgerm agglutinin-conjugated colloidal gold was injected into the stratum lacunosum moleculare of the hippocampal CA1 subfield and into the medial septum. Tritium 116-118 carbonic anhydrase 1 Rattus norvegicus 388-391 12392696-4 2002 Saturation analysis of [3H]testosterone binding showed that brain AR1 levels were significantly reduced (2-3-fold) in either the nuclear or cytosolic fractions of both males and females three weeks after gonadectomy. Tritium 24-26 transcription factor 20 Homo sapiens 66-69 12106612-9 2002 Addition of secretory PLA2 from bee venom to the assay mixture stimulated [3H]AA release, and PAO enhanced the response synergistically. Tritium 75-77 phospholipase A2 group IB Rattus norvegicus 22-26 12063164-6 2002 The increase in [3H]thymidine incorporation and ALPase specific activity were also regulated by protein kinase A (PKA) signaling, since the effects of TGF-beta1 were partially blocked by the PKA inhibitor H-8. Tritium 17-19 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 96-112 12063164-6 2002 The increase in [3H]thymidine incorporation and ALPase specific activity were also regulated by protein kinase A (PKA) signaling, since the effects of TGF-beta1 were partially blocked by the PKA inhibitor H-8. Tritium 17-19 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 114-117 12063164-6 2002 The increase in [3H]thymidine incorporation and ALPase specific activity were also regulated by protein kinase A (PKA) signaling, since the effects of TGF-beta1 were partially blocked by the PKA inhibitor H-8. Tritium 17-19 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 191-194 12089904-3 2002 Mice lacking the GluR epsilon 1 subunit exhibited a malfunction of NMDA receptors, as evidenced by reduction of NMDA receptor channel current, hippocampal long-term potentiation, [3H]MK-801 binding, and NMDA-stimulated 45Ca2+ uptake. Tritium 180-182 glutamate receptor, ionotropic, NMDA2A (epsilon 1) Mus musculus 17-31 11956655-3 2002 11betaHSD1 and 11betaHSD2 activity, as evaluated by the capacity of the microsomal fraction to convert [3H]cortisone to [3H]cortisol and vice versa, was detected in both human adrenal cortex and aldosteronomas, although it was less elevated than in liver and kidneys. Tritium 103-107 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 0-25 11956655-3 2002 11betaHSD1 and 11betaHSD2 activity, as evaluated by the capacity of the microsomal fraction to convert [3H]cortisone to [3H]cortisol and vice versa, was detected in both human adrenal cortex and aldosteronomas, although it was less elevated than in liver and kidneys. Tritium 104-106 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 0-25 11999132-2 2002 We have conducted a detailed pharmacological characterization of the binding of [3H]-PGE2 to recombinant human EP1 prostanoid receptors expressed in human embryonic kidney (HEK-293) cells using a broad panel of natural and synthetic prostanoids. Tritium 81-83 prostaglandin E receptor 1 Homo sapiens 111-114 11944962-5 2002 The Atlantic salmon ER (asER) and rainbow trout ER (rtER) exhibit similar [(3)H]-E2 binding characteristics, although livers from female fish contained a two to three times higher amounts of ER than the males in the two species. Tritium 75-79 estrogen receptor Salmo salar 20-22 12047048-6 2002 Mox-LDL and ox-LDL stimulated [3H]thymidine incorporation with a maximal effect at 5 microg/ml (211%, 154%), which values were significantly greater than that for native LDL (128%). Tritium 31-33 monooxygenase DBH like 1 Homo sapiens 0-3 12047048-9 2002 When added together, mox-LDL (100 ng/ml)-induced [3H]thymidine incorporation was potentiated by low concentrations of 5-HT (1 micromol/l), Ang-II (0.5 micromol/l), ET-1 (1 nmol/l), or U-II (10 nmol/l) (114% to 330%, 325%, 338%, or 345%, respectively). Tritium 50-52 monooxygenase DBH like 1 Homo sapiens 21-24 12047048-9 2002 When added together, mox-LDL (100 ng/ml)-induced [3H]thymidine incorporation was potentiated by low concentrations of 5-HT (1 micromol/l), Ang-II (0.5 micromol/l), ET-1 (1 nmol/l), or U-II (10 nmol/l) (114% to 330%, 325%, 338%, or 345%, respectively). Tritium 50-52 urotensin 2 Homo sapiens 184-188 11834704-7 2002 Exogenous CT-1 markedly stimulated [(3)H]thymidine and [(3)H]proline incorporations (P<0.01), with accumulation of cells in the S phase. Tritium 36-40 cardiotrophin 1 Canis lupus familiaris 10-14 11834704-7 2002 Exogenous CT-1 markedly stimulated [(3)H]thymidine and [(3)H]proline incorporations (P<0.01), with accumulation of cells in the S phase. Tritium 37-40 cardiotrophin 1 Canis lupus familiaris 10-14 11804958-7 2002 Production of PAF and lyso-PAF was measured with a biological assay using [3H]serotonin release from rabbit platelets. Tritium 75-77 PCNA clamp associated factor Homo sapiens 14-17 11804958-7 2002 Production of PAF and lyso-PAF was measured with a biological assay using [3H]serotonin release from rabbit platelets. Tritium 75-77 PCNA clamp associated factor Homo sapiens 27-30 11812233-8 2002 The K(m) of the diacetyl-[(3)H]-Lys-tRNA(Lys) substrate for S. aureus Pth was determined to be 2.8 microM. Tritium 25-31 AT695_RS00135 Staphylococcus aureus 70-73 21179843-7 2002 In the transfected cells with wild MKP-1 gene, Ang II-induced incorporation of 3H-TdR lowered 63%, compared with untransfected cells (P < 0.05). Tritium 79-81 dual specificity phosphatase 1 Rattus norvegicus 35-40 11924870-0 2002 Chronic paroxetine increases [3H]nociceptin binding in rat dorsal raphe nucleus. Tritium 30-32 prepronociceptin Rattus norvegicus 33-43 11924870-3 2002 A significant increase (22-50%) in [3H]nociceptin binding sites density appeared specifically in the dorsal raphe nucleus after 4, 7 or 21 days of treatment, whereas no change was observed at any time in any other brain regions studied. Tritium 36-38 prepronociceptin Rattus norvegicus 39-49 11747988-5 2002 Both fragments expressed as GST-cGB fusion proteins bound cGMP specifically, as determined by competitive [3H]-cGMP ligand binding. Tritium 107-109 glutathione S-transferase kappa 1 Homo sapiens 28-31 12386270-5 2002 Under identical conditions of dose and adjusted specific radioactivity between [(3)H]OCT and [(3)H]1,25(OH)(2)D(3), the plasma concentration of [(3)H]OCT was much lower than that of [(3)H]1,25(OH)(2)D(3). Tritium 81-85 plexin A2 Homo sapiens 150-153 11676490-6 2001 P-selectin affinity chromatography can precipitate a approximately 110-kDa major band and a approximately 220-kDa minor band from [3H]-glucosamine-labeled NCI-H345 cells. Tritium 131-133 selectin P Homo sapiens 0-10 11673895-3 2001 The fusion protein was immobilized in avidin-coated multiwell plates, and varying concentrations of test ligands were allowed to compete with [3H]FK506 for FKBP12 sites on the plate. Tritium 143-145 FKBP prolyl isomerase 1A pseudogene 4 Homo sapiens 156-162 11722589-3 2001 We studied binding of (3)H-labeled hydrophobic hormones (estradiol, progesterone, testosterone, cortisol, aldosterone, and all-trans-retinoic acid) to the inhibitory serpins antithrombin III, heparin cofactor II, plasminogen activator inhibitor-1, and protein C inhibitor (PCI). Tritium 22-26 serpin family C member 1 Homo sapiens 174-190 11602689-6 2001 [3H]PAH was used to show the transport activity of hOAT1 (Km = 3.9 +/-1.3 microM). Tritium 1-3 solute carrier family 22 member 6 Homo sapiens 51-56 11589924-5 2001 Dopamine D1-like receptors, labelled by [3H]-SCH 23390, in young rats displayed a dentate gyrus-CA1 subfield gradient. Tritium 41-43 carbonic anhydrase 1 Rattus norvegicus 96-99 11698075-0 2001 [3H]dofetilide binding to HERG transfected membranes: a potential high throughput preclinical screen. Tritium 1-3 potassium voltage-gated channel subfamily H member 2 Homo sapiens 26-30 11591401-7 2001 MAIN OUTCOME MEASURE(S): We examined basal and IL-3-stimulated cellular proliferation by [3H] thymidine incorporation assay and secretion of beta2-glycoprotein I into culture medium by semiquantitative immunoblot analysis. Tritium 90-92 interleukin 3 Homo sapiens 47-51 12030809-2 2001 Ranked affinities for the inhibition of [3H]nociceptin binding to CTX and CHO"s were: Ac-RYYRWK-NH(2) identical withAc-RYYRIK-NH(2) identical withnociceptin>F/GNC13>Dynorphin A>naloxone. Tritium 41-43 prepronociceptin Rattus norvegicus 44-54 11495676-9 2001 In vitro, gastrin decreased 3H-thymidine labelling in phytohaemagglutinin-pretreated mononuclear cells at a half-maximally effective concentration of 1.5 nM. Tritium 28-30 gastrin Homo sapiens 10-17 12899322-4 2001 RESULTS: IL-10 (10(-8) approximately 10(-10) g/ml) inhibited the increase of 3H-TdR and 3H-proline incorporation as well as FAK activity, which was induced by 10(-7) mol/L AngII (P < 0.05 or P < 0.01). Tritium 77-79 interleukin 10 Rattus norvegicus 9-14 11510482-0 2001 Characterization of specific [3H]nociceptin binding in rat brain and spinal cord. Tritium 30-32 prepronociceptin Rattus norvegicus 33-43 11510482-1 2001 The present study was undertaken to characterize simultaneously [3H]nociceptin binding to opioid receptor-like 1 (ORL1) receptors in the rat brain and spinal cord. Tritium 65-67 prepronociceptin Rattus norvegicus 68-78 11510482-3 2001 The Kd and Bmax values for [3H]nociceptin in the spinal cord were significantly lower than those in the brain. Tritium 28-30 prepronociceptin Rattus norvegicus 31-41 11510482-6 2001 There were regional variations in the specific [3H]nociceptin binding to crude membranes from the rat brain: a relatively high density of [3H]nociceptin binding in the cerebral cortex, hippocampus, thalamus and midbrain, moderately dense binding in the corpus striatum and pons/medulla oblongata, and the lowest density of binding in the cerebellum. Tritium 48-50 prepronociceptin Rattus norvegicus 51-61 11510482-6 2001 There were regional variations in the specific [3H]nociceptin binding to crude membranes from the rat brain: a relatively high density of [3H]nociceptin binding in the cerebral cortex, hippocampus, thalamus and midbrain, moderately dense binding in the corpus striatum and pons/medulla oblongata, and the lowest density of binding in the cerebellum. Tritium 139-141 prepronociceptin Rattus norvegicus 51-61 11510482-6 2001 There were regional variations in the specific [3H]nociceptin binding to crude membranes from the rat brain: a relatively high density of [3H]nociceptin binding in the cerebral cortex, hippocampus, thalamus and midbrain, moderately dense binding in the corpus striatum and pons/medulla oblongata, and the lowest density of binding in the cerebellum. Tritium 139-141 prepronociceptin Rattus norvegicus 142-152 11396983-2 2001 Equilibrium [3H]ryanodine binding assays applied to total membrane fractions from sf 21 cells expressing recombinant RyR1 showed a non-hyperbolic saturation curve (Hill coefficient = 2.1). Tritium 13-15 ryanodine receptor 1 Oryctolagus cuniculus 117-121 11396983-5 2001 The recombinant RyR1 showed a bell-shaped [3H]ryanodine binding curve when free [Ca(2+)] was increased, with an optimal concentration around 100 microM.Confocal microscopy studies using the Ca(2+) ATPase selective inhibitor, thapsigargin coupled to fluorescein and ryanodine coupled to Texas red demonstrated that the recombinant RyR1 and the Ca(2+) ATPase co-localize to the same intracellular membrane. Tritium 43-45 ryanodine receptor 1 Oryctolagus cuniculus 16-20 11356255-3 2001 Stimulation of BAEC with recombinant bovine tumor necrosis factor alpha (rbTNF-alpha) resulted in protein expression of VCAM-1 on less than 5% of all cultured BAECs at 1h post-stimulation, followed by a significant increase at 3h that was maintained until 48h when the proportion of VCAM-1 positive (+) cells decreased significantly, but not to baseline proportions. Tritium 227-229 tumor necrosis factor Bos taurus 44-71 11278552-5 2001 Deletion of PKCgamma had no significant effect on the mRNA product of spinal mu-opioid receptors but caused an increase of maximal binding of the mu-opioid receptor agonist [3H][d-Ala(2),N-Me-Phe(4),Gly(5)-ol]enkephalin in spinal cord membranes obtained from PKCgamma knockout mice. Tritium 174-176 protein kinase C, gamma Mus musculus 12-20 11278552-5 2001 Deletion of PKCgamma had no significant effect on the mRNA product of spinal mu-opioid receptors but caused an increase of maximal binding of the mu-opioid receptor agonist [3H][d-Ala(2),N-Me-Phe(4),Gly(5)-ol]enkephalin in spinal cord membranes obtained from PKCgamma knockout mice. Tritium 174-176 protein kinase C, gamma Mus musculus 259-267 11343685-3 2001 When expressed in monkey COS-7 cells, the human 5-HT(5A) receptor displayed a high affinity for tritiated 5-carbamidotryptamine ([3H]5-CT; K(D)=2.8 nM) and iodinated lysergic acid diethylamide ([125I]LSD; K(D)=187 pM). Tritium 130-132 5-hydroxytryptamine receptor 5A Homo sapiens 48-55 11330337-8 2001 Moreover, the rank order for potency in inhibiting acetylcholinesterase (ambenonium>neostigmine=physostigmine =tacrine>pyridostigmine=edrophonium=galanthamine >desoxypeganine>parathion>gramine) indicated that the most effective inhibitors of acetylcholinesterase also displaced [3H]-oxotremorine-M to the greatest extent. Tritium 294-296 acetylcholinesterase Rattus norvegicus 51-71 11330337-10 2001 However, fasciculin-2, a potent peptide inhibitor of acetylcholinesterase (IC50 24 nM), did prevent catabolism of acetylcholine in rat brain membranes with an atypical inhibition isotherm of [3H]-oxotremorine-M binding, thus permitting an estimation of the "global affinity" of acetylcholine (Ki 85 nM) for [3H]-oxotremorine-M-labelled muscarinic receptors in rat brain. Tritium 192-194 acetylcholinesterase Rattus norvegicus 53-73 11330337-10 2001 However, fasciculin-2, a potent peptide inhibitor of acetylcholinesterase (IC50 24 nM), did prevent catabolism of acetylcholine in rat brain membranes with an atypical inhibition isotherm of [3H]-oxotremorine-M binding, thus permitting an estimation of the "global affinity" of acetylcholine (Ki 85 nM) for [3H]-oxotremorine-M-labelled muscarinic receptors in rat brain. Tritium 308-310 acetylcholinesterase Rattus norvegicus 53-73 11292582-6 2001 In parallel with the behavioral data, autoradiographic analyses of brain sections indicated that the [3H]AMPA binding was decreased in the CA1 and dentate gyrus of the hippocampus and entorhinal cortex in all three Pb-exposed groups. Tritium 102-104 carbonic anhydrase 1 Rattus norvegicus 139-142 11292582-7 2001 However, an increase in [3H]MK801 binding was only observed in CA1 of the hippocampus in the continuously Pb-exposed rats. Tritium 25-27 carbonic anhydrase 1 Rattus norvegicus 63-66 11166283-0 2001 [3H]dofetilide binding in SHSY5Y and HEK293 cells expressing a HERG-like K+ channel? Tritium 1-3 potassium voltage-gated channel subfamily H member 2 Homo sapiens 63-67 11161848-3 2001 The ability of IL-11 to stimulate [3H]thymidine incorporation in IL-11R-expressing ovarian carcinoma cell lines (OVCAR-3 and SKOV-3) and/or abrogate cell death mediated by apoptosis-inducing agents using an ELISA assay that quantitates DNA fragmentation was also studied. Tritium 35-37 interleukin 11 Homo sapiens 15-20 11219446-3 2001 Specific activities of representative villus cell marker enzymes of alkaline phosphatase, aminopeptidase N, sucrase, and lactase increased 6- to 17-fold from F12 (crypt cells) to F1 (villus cells), whereas the crypt cell marker [3H]thymidine incorporation increased 8- to 18-fold from F1 (villus cells) to F12 (crypt cells). Tritium 229-231 LOW QUALITY PROTEIN: lactase-phlorizin hydrolase Sus scrofa 121-128 11160654-6 2001 DH displaced [3H]KA from recombinant GluR5 and GluR6 kainate receptor subunits expressed in HEK293 cells with K(i) values of 0.74 and 1.2 nM, respectively. Tritium 14-16 glutamate ionotropic receptor kainate type subunit 2 Homo sapiens 47-52 11104695-6 2000 Although [(3)H]NAD binding to the enzyme occurred in the absence of spermidine, its affinity for the enzyme was markedly enhanced by spermidine, GC(7) and also by the eIF5A precursor. Tritium 10-14 eukaryotic translation initiation factor 5A Homo sapiens 167-172 11086144-8 2000 Uptake of [3H]all-trans-retinol into the ARPE-19 cells was followed by specific binding of radiolabeled retinoid to RGR. Tritium 11-13 retinal G protein coupled receptor Homo sapiens 116-119 11032985-0 2000 Autoradiographic localization of [3H]nociceptin binding sites in the rat brain. Tritium 34-36 prepronociceptin Rattus norvegicus 37-47 10869358-6 2000 The majority of supplied [(3)H]7K was metabolized beyond 27OH-7K to aqueous-soluble products (apparently bile acids derived from the sterol 27-hydroxylase pathway). Tritium 26-30 cytochrome P450 family 27 subfamily A member 1 Homo sapiens 133-154 11034316-4 2000 Our results showed that MT6-MMP as well as MT4-MMP were labeled with [3H]ethanolamine indicating the presence of a GPI unit with incorporated label. Tritium 70-72 matrix metallopeptidase 17 Homo sapiens 43-50 11074353-4 2000 Both the ER and PR protein concentrations and the [3H]Estradiol binding to the high affinity nuclear receptors decreased after ENK treatment during the first two days. Tritium 51-53 proenkephalin Rattus norvegicus 127-130 10995006-6 2000 We also demonstrated that two of eight cell lines with 11q23 translocation or Ph1 exhibited a significant proliferative response to TPO in the 3H-thymidine uptake and colony-forming assays. Tritium 143-145 alanine--glyoxylate and serine--pyruvate aminotransferase Homo sapiens 78-81 10924015-7 2000 In contrast, [3H]8-OH-DPAT binding in the hippocampal CA1 and CA3 regions was decreased by -9% and -20% from the level of chronic ethanol-treated FH rat (p < 0.05) and returned to the control level seen in FH (naive) rats. Tritium 14-16 carbonic anhydrase 1 Rattus norvegicus 54-57 10844595-8 2000 [3H]-thymidine incorporation in the presence of INF-gamma was significantly lower than that of control in a dose-dependent manner. Tritium 1-3 interferon gamma Rattus norvegicus 48-57 10844595-10 2000 Electroinjected antibody against STAT1 increased [3H]-thymidine incorporation upon stimulation with INF-gamma. Tritium 50-52 interferon gamma Rattus norvegicus 100-109 10844614-4 2000 The activation of phospholipase A2 (PLA2) was assessed by measuring the release of [3H]-arachidonic acid (AA), and changes in intracellular calcium levels were determined by means of confocal microscopy. Tritium 84-86 phospholipase A2 Oryctolagus cuniculus 18-34 10844614-4 2000 The activation of phospholipase A2 (PLA2) was assessed by measuring the release of [3H]-arachidonic acid (AA), and changes in intracellular calcium levels were determined by means of confocal microscopy. Tritium 84-86 phospholipase A2 Oryctolagus cuniculus 36-40 10742305-13 2000 UTP-induced [(3)H]-thymidine incorporation and p42/p44 MAPK phosphorylation was completely inhibited by PD98059 (an inhibitor of MEK1/2). Tritium 13-17 mitogen-activated protein kinase kinase 1 Homo sapiens 129-135 10737595-8 2000 Evidence for G-6-Pase activity was also sought by following the selective detritiation of glucose from the 2-C position when astrocytes were incubated with [2-3H]glucose and [U-14C]glucose in the medium. Tritium 159-161 glucose-6-phosphatase catalytic subunit 1 Rattus norvegicus 13-21 10721818-1 2000 We investigated the mitogenic effect, measured as [3H]thymidine incorporation, of neuropeptide Y (NPY) on smooth muscle cells (SMCs) from human subcutaneous arteries (diameter: 0.4 mm). Tritium 51-53 neuropeptide Y Homo sapiens 82-96 10721818-5 2000 When added together, NPY and NA potentiated the [3H]thymidine incorporation, Emax 109 +/- 38% relative to control. Tritium 49-51 neuropeptide Y Homo sapiens 21-24 10752478-5 2000 Peritumoral injection of various kinds of adjuvant, particularly Freund"s complete adjuvant (FCA) plus bacillus Calmetee-Guerin (BCG), resulted in a marked accumulation of [3H]A-NK cells in mammary tumor tissues 24 h after injection, and simultaneously in the formation of vessels resembling high-endothelium venules (HEV), infiltration of LFA-1+ lymphocytes and expression of the ICAM-1 molecule on the tumor cells in the sites of tumor tissues. Tritium 173-175 integrin subunit alpha L Rattus norvegicus 340-345 10838015-0 2000 Binding of [3H]MSX-2 (3-(3-hydroxypropyl)-7-methyl-8-(m-methoxystyryl)-1-propargylxanthine) to rat striatal membranes--a new, selective antagonist radioligand for A(2A) adenosine receptors. Tritium 12-14 msh homeobox 2 Rattus norvegicus 15-20 10838015-1 2000 The present study describes the preparation and binding properties of a new, potent, and selective A(2A) adenosine receptor (AR) antagonist radioligand, [3H]3-(3-hydroxypropyl)-7-methyl-8-(m-methoxystyryl)-1-propargy lxanth ine ([3H]MSX-2). Tritium 154-156 msh homeobox 2 Rattus norvegicus 233-238 10838015-2 2000 [3H]MSX-2 binding to rat striatal membranes was saturable and reversible. Tritium 1-3 msh homeobox 2 Rattus norvegicus 4-9 10838015-3 2000 Saturation experiments showed that [3H]MSX-2 labeled a single class of binding sites with high affinity (K(d)=8.0 nM) and limited capacity (B(max)=1.16 fmol.mg(-1) of protein). Tritium 36-38 msh homeobox 2 Rattus norvegicus 39-44 10838015-8 2000 [3H]MSX-2 is a highly selective A(2A) AR antagonist radioligand exhibiting a selectivity of at least two orders of magnitude versus all other AR subtypes. Tritium 1-3 msh homeobox 2 Rattus norvegicus 4-9 10566662-6 1999 We found that GM-CSF (0.01-100 ng/mL) has limited mitogenic activity for LSMC but not for MSMC determined by the rate of [3H]thymidine incorporation and cell proliferation assay. Tritium 122-124 colony stimulating factor 2 Homo sapiens 14-20 10516661-12 1999 In contrast, COMT inhibitors, U-0521 and Ro 41-0960, dose-dependently increased intracellular [3H]-NE retention with a maximal increase of 4.5 fold. Tritium 95-97 catechol O-methyltransferase Cricetulus griseus 13-17 10568292-2 1999 Hydrogen-3 and 3H-3He data indicate that the deep basins of the sea are rapidly ventilated, although the hydraulic turnover time of the sea is approximately 200 years. Tritium 15-17 S13 erythroblastosis (avian) oncogene homolog Homo sapiens 64-67 10498842-0 1999 Inhibitory effect of nociceptin on [3H]-5-HT release from rat cerebral cortex slices. Tritium 36-38 prepronociceptin Rattus norvegicus 21-31 10486556-3 1999 [3H]Thymidine incorporation of non-committed mouse P19 embryonal carcinoma cells was inhibited by lithium treatment. Tritium 1-3 cyclin dependent kinase inhibitor 2D Mus musculus 51-54 10480345-4 1999 Thymidylate synthase activity was measured by a tritium-release assay. Tritium 48-55 thymidylate synthetase Homo sapiens 0-20 10403380-2 1999 When 293 cells prelabeled with 13H]AA were incubated with exogenous PUFAs in the presence of IL-1 and serum, there was a significant increase in [3H]AA release (in the order AA > linoleic acid > oleic acid), which was augmented markedly by sPLA2-IIA and modestly by type IV cytosolic PLA2 (cPLA2), but only minimally by type VI Ca2(+)-independent PLA2, overexpression. Tritium 32-34 interleukin 1 alpha Homo sapiens 93-97 10342830-7 1999 The proliferation rate of five activin-secreting clones was measured by [3H]thymidine incorporation and compared with the proliferation rate of untransfected cells. Tritium 73-75 inhibin subunit beta E Homo sapiens 31-38 10336686-5 1999 Phospholipase A2 (2 U/mL), an enzyme that releases AA, and melittin (1 microM), a phospholipase A2 activator, mimicked the AA-facilitation of evoked [3H]GABA release (70 +/- 6% and 76 +/- 7% facilitation, respectively). Tritium 150-152 phospholipase A2 group IB Rattus norvegicus 0-16 10336686-5 1999 Phospholipase A2 (2 U/mL), an enzyme that releases AA, and melittin (1 microM), a phospholipase A2 activator, mimicked the AA-facilitation of evoked [3H]GABA release (70 +/- 6% and 76 +/- 7% facilitation, respectively). Tritium 150-152 phospholipase A2 group IB Rattus norvegicus 82-98 10343284-11 1999 Binding studies with [3H]AVP indicated that the affinity of the mouse V3/V1b receptor (Kd=0.5 nM) is similar to that reported for rat and human receptors. Tritium 22-24 arginine vasopressin receptor 1B Mus musculus 70-76 12212033-2 1999 METHOD: Using experimental models of proliferation of cultured rabbit aortic SMC induced by oxidized-low density lipoprotein, the level of lipoperoxide(LPO) was determined by thiobarbituric acid reactive substance(TBARS) method and 3H-thymidine(3H-TdR) incorporation was used to observe DNA synthesis. Tritium 232-234 lactoperoxidase Oryctolagus cuniculus 152-155 12712718-2 1999 Flavonoid extract of wheat germ resulted to a dose-dependent, time-dependent growth inhibition, reduction of colony and 3H-thymiding incorporation in DNA of human breast cancer cell BCap-37. Tritium 120-122 prohibitin 2 Homo sapiens 182-189 10350488-1 1999 Photoaffinity labeling of the Torpedo nicotinic acetylcholine receptor (nAChR) with [3H]d-tubocurarine (dTC) has identified a residue within the gamma-subunit which, along with the analogous residue in delta-subunit, confers selectivity in binding affinities between the two agonist sites for dTC and alpha-conotoxin (alpha Ctx) MI. Tritium 85-87 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 38-70 10350488-1 1999 Photoaffinity labeling of the Torpedo nicotinic acetylcholine receptor (nAChR) with [3H]d-tubocurarine (dTC) has identified a residue within the gamma-subunit which, along with the analogous residue in delta-subunit, confers selectivity in binding affinities between the two agonist sites for dTC and alpha-conotoxin (alpha Ctx) MI. Tritium 85-87 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 72-77 10350488-2 1999 nAChR gamma-subunit, isolated from nAChR-rich membranes photolabeled with [3H]dTC, was digested with Staphylococcus aureus V8 protease, and a 3H-labeled fragment was purified by reversed-phase high-performance liquid chromatography. Tritium 75-77 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 0-5 10350488-2 1999 nAChR gamma-subunit, isolated from nAChR-rich membranes photolabeled with [3H]dTC, was digested with Staphylococcus aureus V8 protease, and a 3H-labeled fragment was purified by reversed-phase high-performance liquid chromatography. Tritium 142-144 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 0-5 10505064-3 1999 Binding of [3H]PGF2 alpha and [3H]PGD2 was inhibited by non-radioactive PGF2 alpha (IC50 values of 21 and 9 nmol l-1, respectively), PGD2 (35 and 21 nmol l-1), and PGE2 (223 and 81 nmol l-1), but not by PGE1 (> 10,000 and 5616 nmol l-1). Tritium 12-14 prostaglandin F synthase 2 Bos taurus 15-19 10505064-3 1999 Binding of [3H]PGF2 alpha and [3H]PGD2 was inhibited by non-radioactive PGF2 alpha (IC50 values of 21 and 9 nmol l-1, respectively), PGD2 (35 and 21 nmol l-1), and PGE2 (223 and 81 nmol l-1), but not by PGE1 (> 10,000 and 5616 nmol l-1). Tritium 12-14 prostaglandin F synthase 2 Bos taurus 72-76 10505064-3 1999 Binding of [3H]PGF2 alpha and [3H]PGD2 was inhibited by non-radioactive PGF2 alpha (IC50 values of 21 and 9 nmol l-1, respectively), PGD2 (35 and 21 nmol l-1), and PGE2 (223 and 81 nmol l-1), but not by PGE1 (> 10,000 and 5616 nmol l-1). Tritium 31-33 prostaglandin F synthase 2 Bos taurus 15-19 10505064-3 1999 Binding of [3H]PGF2 alpha and [3H]PGD2 was inhibited by non-radioactive PGF2 alpha (IC50 values of 21 and 9 nmol l-1, respectively), PGD2 (35 and 21 nmol l-1), and PGE2 (223 and 81 nmol l-1), but not by PGE1 (> 10,000 and 5616 nmol l-1). Tritium 31-33 prostaglandin F synthase 2 Bos taurus 72-76 10505064-4 1999 In contrast, [3H]PGE1 was inhibited by non-radioactive PGE1 (14 nmol l-1) and PGE2 (7 nmol l-1), but minimally by PGD2 (2316 nmol l-1) and PGF2 alpha (595 nmol l-1). Tritium 14-16 prostaglandin F synthase 2 Bos taurus 139-143 10231436-7 1999 Using similar incubation conditions, IL-1 also increased [3H]AA release from MCs. Tritium 58-60 interleukin 1 alpha Homo sapiens 37-41 10471125-3 1999 Fibroblasts deficient in long-chain acyl coenzyme A dehydrogenase (LCAD) converted [3H]20:4 to [3H]20:3. Tritium 84-86 acyl-CoA dehydrogenase long chain Homo sapiens 25-65 10471125-3 1999 Fibroblasts deficient in long-chain acyl coenzyme A dehydrogenase (LCAD) converted [3H]20:4 to [3H]20:3. Tritium 84-86 acyl-CoA dehydrogenase long chain Homo sapiens 67-71 10471125-3 1999 Fibroblasts deficient in long-chain acyl coenzyme A dehydrogenase (LCAD) converted [3H]20:4 to [3H]20:3. Tritium 96-98 acyl-CoA dehydrogenase long chain Homo sapiens 25-65 10471125-3 1999 Fibroblasts deficient in long-chain acyl coenzyme A dehydrogenase (LCAD) converted [3H]20:4 to [3H]20:3. Tritium 96-98 acyl-CoA dehydrogenase long chain Homo sapiens 67-71 10471125-5 1999 [3H]Hexadecatrienoic acid (16:3) was the main product that accumulated when [3H]20:4 was incubated with normal, LCAD deficient, and very long-chain acyl coenzyme A dehydrogenase (VLCAD) deficient fibroblasts, but Zellweger fibroblasts did not form this product. Tritium 1-3 acyl-CoA dehydrogenase very long chain Homo sapiens 132-177 10471125-5 1999 [3H]Hexadecatrienoic acid (16:3) was the main product that accumulated when [3H]20:4 was incubated with normal, LCAD deficient, and very long-chain acyl coenzyme A dehydrogenase (VLCAD) deficient fibroblasts, but Zellweger fibroblasts did not form this product. Tritium 1-3 acyl-CoA dehydrogenase very long chain Homo sapiens 179-184 10216201-5 1999 Phospholipase A2 (PLA2, 2 U/ml), an enzyme that releases AA from the sn-2 position of phospholipids, mimicked the facilitatory effect of AA on the evoked tritium release (86+/-14% facilitation), an effect prevented by BSA (1%). Tritium 154-161 phospholipase A2 group IB Rattus norvegicus 0-16 17603034-5 2007 Insulin (0.1 and 1 microM) caused an enhancement of [(3)H]-glucose uptake into adipocytes with a greater magnitude in +db/+m mice. Tritium 52-59 insulin I Mus musculus 0-18 17640952-6 2007 Samples of frontal cortex were obtained for each animal immediately following behavioral testing, and 5-HT2A receptor density was measured using [3H]ketanserin. Tritium 146-148 5-hydroxytryptamine receptor 2A Oryctolagus cuniculus 102-117 17373911-7 2007 [(3)H]IP(3) (inositol 1,4,5-trisphosphate) binding affinity and IP(3)-induced Ca2+ release activity were enhanced in permeabilized cells that were pre-treated with forskolin or PKA. Tritium 0-5 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 177-180 17361011-2 2007 Enzyme activity was determined using a 333 bp polymerase chain reaction product of the estrogen receptor-alpha gene that contained either radiolabeled cytosine or tritium-labeled methyl groups at CpG sites. Tritium 163-170 estrogen receptor 1 (alpha) Mus musculus 87-110 17442507-5 2007 Treatment with DZN significantly decreased the activity of AChE in brain of diabetic rats than normal rats 3h after injection (65 mg/kg), although no significant difference in the activity was found between normal and diabetic rats. Tritium 107-109 acetylcholinesterase Rattus norvegicus 59-63 17275920-3 2007 The c-FLIP competitively inhibited the recruitment of caspase-8 to the death-inducing signaling complex (DISC), which took as long as 3h to form after the stimulation of Fas in A20 cells. Tritium 134-136 CASP8 and FADD-like apoptosis regulator Mus musculus 4-10 17275920-3 2007 The c-FLIP competitively inhibited the recruitment of caspase-8 to the death-inducing signaling complex (DISC), which took as long as 3h to form after the stimulation of Fas in A20 cells. Tritium 134-136 caspase 8 Mus musculus 54-63 17242285-4 2007 METHODS AND RESULTS: It is demonstrated that 6-MP increases Nur77 activity in cultured SMCs, which results in reduced [3H]thymidine incorporation, whereas Nur77 small interfering RNA knockdown partially restores DNA synthesis. Tritium 119-121 nuclear receptor subfamily 4, group A, member 1 Mus musculus 60-65 16989644-7 2007 Both peptides increased [3H]ryanodine binding to rabbit RyR1s, increased Ca2+ release from sarcoplasmic reti-culum vesicles and increased single RyR1 channel activity. Tritium 25-27 ryanodine receptor 1 Oryctolagus cuniculus 56-60 16989644-9 2007 [3H]Ryanodine binding to recombinant ASI(+)RyR1 or ASI(-)-RyR1 was enhanced more by PASI(-) than PASI(+), with the greatest increase seen when PASI(-) was added to ASI(-)RyR1. Tritium 1-3 ryanodine receptor 1 Oryctolagus cuniculus 43-47 16989644-9 2007 [3H]Ryanodine binding to recombinant ASI(+)RyR1 or ASI(-)-RyR1 was enhanced more by PASI(-) than PASI(+), with the greatest increase seen when PASI(-) was added to ASI(-)RyR1. Tritium 1-3 ryanodine receptor 1 Oryctolagus cuniculus 58-62 16989644-9 2007 [3H]Ryanodine binding to recombinant ASI(+)RyR1 or ASI(-)-RyR1 was enhanced more by PASI(-) than PASI(+), with the greatest increase seen when PASI(-) was added to ASI(-)RyR1. Tritium 1-3 ryanodine receptor 1 Oryctolagus cuniculus 58-62 17122151-11 2006 Competition studies showed marked inhibition of [3H]-PTZ binding in the presence of sigmaR1-specific ligands. Tritium 49-51 sigma non-opioid intracellular receptor 1 Mus musculus 84-91 17072262-11 2006 Leptin (10 nmol/L - 2 umol/L) caused a significant increase in the activation of PI (3)-K that was accompanied by an increase in cell proliferation dose dependently based on the [3H]-thymidine incorporation. Tritium 179-181 leptin Homo sapiens 0-6 16343502-7 2006 hU-II increased the 3H thymidine incorporation into DNA in a concentration-dependent manner. Tritium 20-22 urotensin 2 Homo sapiens 0-5 17283870-5 2006 On interaction with mox-LDL or H2O2, U-II induced the greatest increase in [3H]thymidine incorporation among these vasoactive agents. Tritium 76-78 monooxygenase DBH like 1 Homo sapiens 20-23 17042265-1 2006 We found that the tritium-labeled synthetic ACTH-like octapeptide leucocorticotropin corresponding to the 81-88 sequence of the precursor of human interleukin-1alpha ([3H]GKVLKKRR) is bound by the ACTH receptor of rat adrenal cortex with a high affinity and specificity (Kd 2.2 +/- 0.1 nM). Tritium 18-25 interleukin 1 alpha Homo sapiens 147-165 16728647-11 2006 beta4 Gene deletion partially reduced cytisine-resistant and alpha-bungarotoxin-resistant sites with lower and higher affinity for [3H]epibatidine, respectively. Tritium 132-134 basic helix-loop-helix family, member e23 Mus musculus 0-5 16728647-15 2006 These studies establish that the lower-affinity sites represent a structurally diverse set of sites that require expression of either alpha7, beta2, or beta4 subunits and extend and confirm previous classifications of the higher-affinity [3H]epibatidine binding sites. Tritium 239-241 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 134-140 16728647-15 2006 These studies establish that the lower-affinity sites represent a structurally diverse set of sites that require expression of either alpha7, beta2, or beta4 subunits and extend and confirm previous classifications of the higher-affinity [3H]epibatidine binding sites. Tritium 239-241 basic helix-loop-helix family, member e23 Mus musculus 152-157 16908848-4 2006 Incubation of purified Bet3 with [3H]palmitoyl-CoA (Pal-CoA) leads to a rapid and stoichiometric attachment of fatty acids to cysteine-68. Tritium 34-36 trafficking protein particle complex subunit 3 Homo sapiens 23-27 16759729-5 2006 In both adult and aged gerbils, alpha-synuclein immunoreactivity and level started to increase 3h after ischemia, and they were highest 1 day after ischemia. Tritium 95-97 synuclein alpha Homo sapiens 32-47 16674925-3 2006 Capsaicin at concentrations ranging from 10 to 100 microM, which were found to be non-cytotoxic towards the Caco-2 cells, were observed to inhibit P-gp mediated efflux transport of [3H]-digoxin in the cells. Tritium 182-184 phosphoglycolate phosphatase Homo sapiens 147-151 16556605-4 2006 In this study, to analyze the posttranslational N-myristoylation of gelsolin during apoptosis, metabolic labeling of gelsolin and its caspase cleavage products expressed in COS-1 cells with [3H]myristic acid was performed. Tritium 191-193 gelsolin Homo sapiens 117-125 16547349-6 2006 The Ki values of A1 and A2 (CB2 approximately 60 nM; CB1 >1500 nM) were determined by displacement of the synthetic high affinity cannabinoid ligand [3H]CP-55,940. Tritium 153-155 cannabinoid receptor 2 Homo sapiens 28-31 16501582-0 2006 [3H]GR205171 displays similar NK1 receptor binding profile in gerbil and human brain. Tritium 1-3 tachykinin receptor 1 Homo sapiens 30-42 16501582-1 2006 1 In this study, [(3)H]GR205171 (3(S)-(2-methoxy-5-(5-trifluoromethyltetrazol-1-yl)-phenylmethylamino)-2(S)-phenylpiperidine), a potent and selective NK1 receptor antagonist, was characterised in autoradiographic studies in gerbil brain and in binding experiments on homogenates from gerbil and human brain cortex and striatum. Tritium 17-22 tachykinin receptor 1 Homo sapiens 150-162 16724555-3 2006 Zalcitabine exhibited the inhibition effect on the cellular uptake of [3H]-PAH in CHO/hOAT1 cells with an IC50 value of 1.23 mM. Tritium 71-73 solute carrier family 22 member 6 Homo sapiens 86-91 16624955-9 2006 Both EMs displaced binding of [3H]-[D-Ala2,N-Me-Phe4,Gly5-ol]enkephalin to mu-receptors in dorsal root ganglia (DRG). Tritium 31-33 proenkephalin Rattus norvegicus 61-71 16289537-4 2006 The hPHT1 full-sequence was amplified from BeWo cells, inserted into the pcDNA3.1-V5/His TOPO plasmid and transiently transfected into COS-7 cells to investigate the uptake kinetics of [3H]histidine and [3H]carnosine. Tritium 186-188 solute carrier family 15 member 4 Homo sapiens 4-9 16436281-2 2006 To gain insight into the structural features associated with the activated form of Itk, we have solved the NMR structure of the SH2 domain bound to a phosphotyrosine-containing peptide (pY) and analyzed changes in trans-hydrogen bond scalar couplings ((3h)J(NC")) that result from pY binding. Tritium 253-255 IL2 inducible T cell kinase Homo sapiens 83-86 16322064-7 2006 CRF increased, but stable CRF-BP overexpression decreased, [3H]thymidine incorporation in XLT-15 cells. Tritium 60-62 corticotropin releasing hormone binding protein S homeolog Xenopus laevis 26-32 16429444-8 2006 We found decreases in [3H]MK801 binding in the hippocampus CA1 region by LPS-treatment for 5 days. Tritium 23-25 carbonic anhydrase 1 Rattus norvegicus 59-62 16316670-0 2006 Use of an inverse agonist radioligand [3H]A-317920 reveals distinct pharmacological profiles of the rat histamine H3 receptor. Tritium 39-41 histamine receptor H3 Rattus norvegicus 104-125 16376323-8 2006 A statistically significant increase in cell size, [3H]leucine incorporation, and expression of well-characterized markers of cardiac hypertrophy, namely cardiac alpha-actin and myosin light chain, were observed in response to leptin. Tritium 52-54 leptin Homo sapiens 227-233 16192276-8 2005 The amount and activity of a key enzyme of the CDP-ethanolamine pathway for PE synthesis, CTP:phosphoethanolamine cytidylyltransferase, were increased by 35-40 and 100%, respectively, in tissues of Pisd+/- mice, as judged by immunoblotting; PE synthesis from [3H]ethanolamine was correspondingly increased in hepatocytes. Tritium 260-262 cut-like homeobox 1 Mus musculus 47-50 16095762-4 2005 The affinity and selectivity to the VAChT were analysed by competitive binding studies using tritium labelled radioligands. Tritium 93-100 solute carrier family 18 member A3 Homo sapiens 36-41 16150932-1 2005 The function of GAT1, the transporter for the inhibitory neurotransmitter GABA, is characterized by expression in Xenopus laevis oocytes and measurements of GABA-induced uptake of [3H]GABA, 22Na+, and 36Cl-, and GABA-evoked currents under voltage-clamp conditions. Tritium 181-183 solute carrier family 6 (neurotransmitter transporter), member 1 S homeolog Xenopus laevis 16-20 16251407-5 2005 Interferon (IFN)-gamma, which signals growth arrest via Stat1, inhibited EGF- or PDGF-stimulated mitogenesis in wild-type fibroblasts but enhanced [3H]thymidine uptake in Stat1-/- fibroblasts. Tritium 148-150 epidermal growth factor Mus musculus 73-77 15916532-2 2005 Here we show that recombinant CLIC-2 increases [3H]ryanodine binding to native and purified RyR channels, enhances substate activity in individual channels, increases the number of rare coupled gating events between associated RyRs, and reduces activation of the channels by their primary endogenous cytoplasmic ligands, ATP and Ca2+. Tritium 48-51 chloride intracellular channel 2 Homo sapiens 30-36 16091787-3 2005 The residence time of both radioactivities in plasma was utilized for the simultaneous calculation of the particle remnant removal rate and of the lipoprotein lipase activity expressed as a delipidation index = 1 - [(3H) triolein residence time/(14C) cholesteryl oleate residence time]. Tritium 217-219 lipoprotein lipase Homo sapiens 147-165 15573401-7 2005 Pretreatment with U0126 (a MEK1/2 inhibitor) attenuated the p42/p44 MAPK phosphorylation and [3H]thymidine incorporation stimulated by BK, but had no effect on Akt activation. Tritium 94-96 mitogen-activated protein kinase kinase 1 Homo sapiens 27-33 15789377-8 2005 The glutamate transporter blocker DL-TBOA or dihydrokainate inhibited in part (approximately 35%) the GABA (10 microM)-evoked [3H]D-ASP release; this release was strongly reduced by the anion channel blockers niflumic acid and NPPB. Tritium 127-129 natriuretic peptide type B Mus musculus 227-231 15729740-3 2005 [3H]MeNER binding in the wildtype mouse brains was consistent with previously reported distributions of NET. Tritium 1-3 solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2 Mus musculus 104-107 15729740-7 2005 [3H]MeNER displayed much higher affinity for NET than for SERT or DAT in homogenate binding studies. Tritium 1-3 solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2 Mus musculus 45-48 15713670-6 2005 Experiments using a pull-down assay with [3H]EGCG demonstrate binding of EGCG to vimentin with a Kd of 3.3 nm. Tritium 42-44 vimentin Homo sapiens 81-89 15511219-3 2005 Metabolic labelling of HEK-293 (human embryonic kidney) cells showed the incorporation of [3H]myristate into CHMP6 fused C-terminally to GFP (green fluorescent protein) (CHMP6-GFP). Tritium 91-93 charged multivesicular body protein 6 Homo sapiens 109-114 15511219-3 2005 Metabolic labelling of HEK-293 (human embryonic kidney) cells showed the incorporation of [3H]myristate into CHMP6 fused C-terminally to GFP (green fluorescent protein) (CHMP6-GFP). Tritium 91-93 charged multivesicular body protein 6 Homo sapiens 170-175 15608129-3 2005 The assay is based on the release of tritium as tritiated water that occurs upon CYP2C9-mediated 4"-hydroxylation of diclofenac labeled with tritium in the 4" position. Tritium 37-44 cytochrome P450 family 2 subfamily C member 9 Homo sapiens 81-87 15608129-3 2005 The assay is based on the release of tritium as tritiated water that occurs upon CYP2C9-mediated 4"-hydroxylation of diclofenac labeled with tritium in the 4" position. Tritium 141-148 cytochrome P450 family 2 subfamily C member 9 Homo sapiens 81-87 15358595-6 2005 Inhibition of phosphatidylinositol (PI)3-kinase with LY-294002 or mTOR/p70S6K1 with rapamycin reduced [3H]thymidine incorporation by 50%, i.e., to levels comparable to those achieved by addition of either AG-1478 or GM-6001. Tritium 103-105 ribosomal protein S6 kinase B1 Rattus norvegicus 71-78 16492548-9 2005 When muscle glycogen was pre-labeled with tritium in the three position, amylin could be shown to evoke the release of free glucose. Tritium 42-49 islet amyloid polypeptide Rattus norvegicus 73-79 15479160-3 2004 Incubation of recombinant hYkt6 with [3H]Pal-CoA ([3H]palmitoyl-CoA) leads to covalent attachment of palmitate to C-terminal cysteine residues. Tritium 38-40 YKT6 v-SNARE homolog Homo sapiens 26-31 15479160-3 2004 Incubation of recombinant hYkt6 with [3H]Pal-CoA ([3H]palmitoyl-CoA) leads to covalent attachment of palmitate to C-terminal cysteine residues. Tritium 51-53 YKT6 v-SNARE homolog Homo sapiens 26-31 15569254-3 2004 Membranes prepared from cells expressing either SER-2 or SER-2A bind [3H]lysergic acid diethylamide (LSD) in the low nanomolar range and exhibit highest affinity for tyramine. Tritium 70-72 G_PROTEIN_RECEP_F1_2 domain-containing protein;Tyramine receptor Ser-2 Caenorhabditis elegans 48-53 15569254-3 2004 Membranes prepared from cells expressing either SER-2 or SER-2A bind [3H]lysergic acid diethylamide (LSD) in the low nanomolar range and exhibit highest affinity for tyramine. Tritium 70-72 G_PROTEIN_RECEP_F1_2 domain-containing protein;Tyramine receptor Ser-2 Caenorhabditis elegans 57-62 19641719-4 2004 Various concentrations of IGF-1, ranging from 0.1 nM to 10 nM, increased [(3)H]-leucine incorporation into the TCA-insoluble fraction of cardiomyocytes in a dose-dependent manner. Tritium 73-78 insulin-like growth factor 1 Rattus norvegicus 26-31 15339391-7 2004 U0126, an MEK1/2 inhibitor, increased CT-1-induced the phosphorylation of STAT3 in a dose-dependent manner and, consistently, augmented CT-1-induced increase in [3H]leucine incorporation and cellular protein-to-DNA ratio by 17.6 % and 16.3 %, respectively. Tritium 162-164 signal transducer and activator of transcription 3 Rattus norvegicus 74-79 14985920-11 2004 Moreover, the in vivo [3H](+)-SKF-10,047 binding levels to the sigma1 receptor were increased after cocaine conditioning in numerous brain structures and these increases subsisted after extinction. Tritium 23-25 sigma non-opioid intracellular receptor 1 Mus musculus 63-78 15571037-4 2004 By inhibition of poly(ADP-ribose) polymerase (PARP) with 3-aminobenzamidine and treatment of ADP-ribosylated proteins with phosphodiesterase I, it was demonstrated that about 90% of [3H]-adenin bound by proteins in the nuclei and 70% in the mitochondria was the result of PARP activity. Tritium 183-185 poly (ADP-ribose) polymerase 1 Rattus norvegicus 17-44 15157672-4 2004 Concomitantly, alpha2M*-induced increase in [3H]thymidine uptake was abolished in these transfected cells. Tritium 45-47 alpha-2-macroglobulin Homo sapiens 15-22 15024037-0 2004 [3H]A-317491, a novel high-affinity non-nucleotide antagonist that specifically labels human P2X2/3 and P2X3 receptors. Tritium 1-3 purinergic receptor P2X 2 Homo sapiens 93-99 15024037-2 2004 In the present studies, the ability of [3H]A-317491 to label recombinant human P2X(2/3) and P2X(3) receptors was characterized. Tritium 40-42 purinergic receptor P2X 2 Homo sapiens 79-86 15272206-6 2004 Moreover, the [(3)H]astemizole binding data demonstrated a rank order of affinity that was highly correlated to that of inhibitory potency in the electrophysiological studies for HERG in HEK293 (r(SP) = 0.91, P<0.05). Tritium 14-19 potassium voltage-gated channel subfamily H member 2 Homo sapiens 179-183 15126126-3 2004 Using in vitro receptor autoradiography, we found that calcium treatment of C57 mice sections resulted in a marked increase of 3H-AMPA binding in areas CA3 and CA1 of the hippocampus and in the dentate gyrus. Tritium 127-129 carbonic anhydrase 1 Mus musculus 160-163 15253857-5 2004 METHODS: [3H] thymidine incorporation assay was used to assess mouse T-cell proliferation after stimulation with latex beads coated with anti-CD3/CD28 antibodies. Tritium 10-12 CD28 antigen Mus musculus 146-150 15155838-4 2004 [3H]GABA uptake by the GAT-4 E61G mutant becomes markedly more sensitive to inhibition by taurine (IC50 approximately 0.26 mM). Tritium 1-3 solute carrier family 6 member 11 Homo sapiens 23-28 15154814-6 2004 In [Ni(cyclam)][Ru(ox)(NO)Cl(3)].3H(2)O the enthalpy maximum of the thermal decay of SII appears at 182 K, which is a relatively high decay temperature for SII. Tritium 33-35 transcription elongation factor A1 Homo sapiens 85-88 15154814-6 2004 In [Ni(cyclam)][Ru(ox)(NO)Cl(3)].3H(2)O the enthalpy maximum of the thermal decay of SII appears at 182 K, which is a relatively high decay temperature for SII. Tritium 33-35 transcription elongation factor A1 Homo sapiens 156-159 15120574-0 2004 [(3)H]Adenosine uptake in brainstem membranes of CD-1 mice lacking the adenosine A(2a) receptor. Tritium 0-5 CD1 antigen complex Mus musculus 49-53 15344652-0 2004 [Leu-enkephalin homogeneously labeled with tritium in studying the Selank inhibiting effect on the enkephalin-degrading enzymes of human plasma]. Tritium 43-50 prodynorphin Homo sapiens 1-15 15344652-1 2004 A method of analysis of enkephalinase activity in blood plasma based on the application of Leu-enkephalin generally labeled with tritium at all its amino acid residues was developed. Tritium 129-136 prodynorphin Homo sapiens 91-105 15344660-1 2004 Competition of a number of progesterone 16alpha,17alpha-cycloalkane derivatives with 3H-labeled ligands for the binding sites of the rat uterine progesterone receptor, uterine pentaranophilin, and blood serum pentaranophilin was studied. Tritium 85-87 progesterone receptor Rattus norvegicus 145-166 15081270-5 2004 We found that all the OC pesticides tested were able to shift the agonist [(3)H]-R1881 from its binding site to the AR in competitive binding assays. Tritium 75-79 lymphatic vessel endothelial hyaluronan receptor 1 Homo sapiens 116-118 14709337-2 2004 Stimulation of 1-LN cells with activated alpha2-macroglobulin (alpha2M*) caused a two- to threefold increase in [3H]thymidine uptake and cell number. Tritium 113-115 alpha-2-macroglobulin Homo sapiens 41-61 14709337-2 2004 Stimulation of 1-LN cells with activated alpha2-macroglobulin (alpha2M*) caused a two- to threefold increase in [3H]thymidine uptake and cell number. Tritium 113-115 alpha-2-macroglobulin Homo sapiens 63-71 15171952-4 2004 In a subsequent radioligand binding assay, the binding of [3H]Ang II to the fibroblasts was specific and saturable with both high- and low-affinity sites. Tritium 59-61 angiogenin Oryctolagus cuniculus 62-65 15171952-5 2004 Competition binding experiments indicated that Ang II completely displaced [3H]Ang II binding, and CV-11974 and PD123,319 maximally displaced up to approximately 63% and 37% of the total binding, respectively. Tritium 76-78 angiogenin Oryctolagus cuniculus 47-50 15171952-5 2004 Competition binding experiments indicated that Ang II completely displaced [3H]Ang II binding, and CV-11974 and PD123,319 maximally displaced up to approximately 63% and 37% of the total binding, respectively. Tritium 76-78 angiogenin Oryctolagus cuniculus 79-82 15171952-6 2004 Ang II and CV-11974 completely displaced the [3H]DuP753 binding but PD123,319 did not, indicating a single population of binding site. Tritium 46-48 angiogenin Oryctolagus cuniculus 0-3 14667929-9 2004 Pretreatment of HUVEC with a p21 antisense oligonucleotide reversed the DPTH-induced inhibition of [3H]thymidine incorporation into HUVEC. Tritium 100-102 H3 histone pseudogene 16 Homo sapiens 29-32 15325959-3 2004 In the rat, consolidation of the long-term memory for a one-trial, step-down inhibitory avoidance task is blocked by antagonists of the rNMDA and rAMPA infused into the CA1 region of the dorsal hippocampus early after training and is associated with a rapid and reversible increase in the total number of [3H]AMPA binding sites. Tritium 306-308 carbonic anhydrase 1 Rattus norvegicus 169-172 14625441-2 2003 The inhibition of electrically evoked [3H]Ch efflux by nociceptin (0.03-3 microM) was moderate (max -33%), more evident in the neocortex than in the hippocampus and was prevented by [Nphe1]NC(1-13)NH(2) 10 microM. Tritium 39-41 prepronociceptin Rattus norvegicus 55-65 14709851-4 2003 An overexpression of hPTTG significantly inhibited cell growth, which was determined by the adherent cell growth properties, colony formation in soft agar and [3H] thymidine incorporation, respectively, in HeLa and A549 cells. Tritium 160-162 PTTG1 regulator of sister chromatid separation, securin Homo sapiens 21-26 14534366-2 2003 In an attempt to better understand the unique pharmacological profile of [Dmt1]DALDA, we have prepared [3H][Dmt1]DALDA and compared its binding properties with that of [3H]DAMGO ([d-Ala2,N-Me-Phe4,Gly5-ol]-enkephalin). Tritium 104-106 doublesex and mab-3 related transcription factor 1 Homo sapiens 108-112 12946450-7 2003 Potency at the recombinant rabbit B2 receptor was generally decreasing in the series of four drugs (Ki in a [3H]BK competition assay to recombinant receptors of 0.78, 0.77, 10.2 and 44.4 nM, respectively); these four compounds did not displace [3H]Lys-des-Arg(9)-BK binding from human B1 receptors expressed by smooth muscle cells. Tritium 109-111 bradykinin receptor B2 Homo sapiens 34-45 12946450-7 2003 Potency at the recombinant rabbit B2 receptor was generally decreasing in the series of four drugs (Ki in a [3H]BK competition assay to recombinant receptors of 0.78, 0.77, 10.2 and 44.4 nM, respectively); these four compounds did not displace [3H]Lys-des-Arg(9)-BK binding from human B1 receptors expressed by smooth muscle cells. Tritium 245-247 bradykinin receptor B2 Homo sapiens 34-45 12922933-0 2003 In vitro and ex vivo effects of a selective nociceptin/orphanin FQ (N/OFQ) peptide receptor antagonist, CompB, on specific binding of [3H]N/OFQ and [35S]GTPgammaS in rat brain and spinal cord. Tritium 135-137 prepronociceptin Rattus norvegicus 44-54 12922933-0 2003 In vitro and ex vivo effects of a selective nociceptin/orphanin FQ (N/OFQ) peptide receptor antagonist, CompB, on specific binding of [3H]N/OFQ and [35S]GTPgammaS in rat brain and spinal cord. Tritium 135-137 prepronociceptin Rattus norvegicus 55-66 12942748-2 2003 Enkephalinase activity was assayed in vitro by the rate of 3H-Leu-enkephalin hydrolysis in the presence of the tested peptides. Tritium 59-61 proenkephalin Rattus norvegicus 66-76 12942748-6 2003 The interaction between the peptides and opioid receptors was estimated using a radioreceptor method with [3H][D-Ala2, D-Leu5]-enkephalin. Tritium 107-109 proenkephalin Rattus norvegicus 127-137 12657653-11 2003 The specific binding of [3H]ATMP with K5 or ovalbumin was confirmed by Scatchard analysis. Tritium 25-27 keratin 5 Homo sapiens 38-53 12670875-5 2003 The ability of endostatin to reduce VEGF-induced retinal vascular permeability was confirmed by using [3H]mannitol leakage and two other parameters, fluorescein leakage and retinal thickness, after subretinal injection of a bovine immunodeficiency lentiviral vector coding for endostatin (BIV-vectored endostatin, or BIVendostatin). Tritium 103-105 collagen type XVIII alpha 1 chain Homo sapiens 15-25 12531391-8 2003 In situ hybridization showed higher levels of OGG1 mRNA expression in the renal tubules in the OM than in the cortex of the normal kidney, which decreased rapidly within 3h of I/R. Tritium 170-172 8-oxoguanine DNA glycosylase Rattus norvegicus 46-50 12856821-7 2003 Furthermore, factor analysis showed that rat conditioned freezing behavior and [3H]-epibatidine binding in the CA1 layer of the hippocampus and enorhinal cortex, represented similar central processes. Tritium 80-82 carbonic anhydrase 1 Rattus norvegicus 111-114 12242100-3 2002 Activin A stimulated FSH release and effect appeared 1h after injection (168% increase of controls) reaching a maximum at 3h (437% of controls). Tritium 122-124 inhibin subunit beta A Rattus norvegicus 0-9 12175707-9 2002 Effects of Ucn on [(3)H]-thymidine uptake into NMCs were significantly abolished by the protein kinase A inhibitor, H89 (10(-5) M), but not by a competitive antagonist of CRH receptors, astressin (10(-5) M). Tritium 19-23 urocortin Homo sapiens 11-14 12167464-2 2002 [3H]Desloratadine bound to membranes expressing the recombinant human histamine H(1) receptor in Chinese hamster ovary cells (CHO-H(1)) in a specific and saturable manner with a K(d) of 1.1+/-0.2 nM, a B(max) of 7.9+/-2.0 pmol/mg protein, and an association rate constant of 0.011 nM(-1) x min(-1). Tritium 1-3 histamine receptor H1 Homo sapiens 70-93 12109593-7 2002 11betaHSD1 and 11betaHSD2 activity was assayed by measuring the capacity of both microsomal fraction and tissue fragments to convert [3H]cortisone to [3H]cortisol and vice versa. Tritium 134-136 hydroxysteroid 11-beta dehydrogenase 2 Homo sapiens 15-25 11958952-8 2002 Both S-thiolation reaction of GBP in synaptic membranes with [35S]cystine and reaction of Cys residues in GBP with [3H]NEM were significantly decreased after exposure of membranes to O(-)(2). Tritium 116-118 transmembrane protein 132A Homo sapiens 106-109 12047048-7 2002 5-HT, Ang-II, ET-1, or U-II also stimulated [3H]thymidine incorporation in a dose-dependent manner. Tritium 45-47 urotensin 2 Homo sapiens 23-27 11911959-5 2002 Study of leptin effects on human breast cancer cells growth was performed by [3H]-thymidine incorporation method and colorimetric MTT assay. Tritium 78-80 leptin Homo sapiens 9-15 21179843-2 2002 We also investigated the effect of MKP-1 genes,which were transfected into vascular smooth muscle cells (VSMC) using the classical calcium phosphate coprecipitation technique, on the incorporation of 3H-TdR in VSMC stimulated by angiotensin II (Ang II). Tritium 200-202 dual specificity phosphatase 1 Rattus norvegicus 35-40 11793021-6 2001 Clusterin cDNA was transfected to MIN6 cells and their proliferation activity was measured by a [3H]thymidine-incorporation assay. Tritium 97-99 clusterin Mus musculus 0-9 11770002-4 2001 The characteristics of hEMT-mediated uptake of [3H]1-methyl-4-phenylpyridinium ([3H]MPP+) were studied by incubating the cells at 37 degrees C for 1 min with 200 nM [3H]MPP+. Tritium 48-50 IL2 inducible T cell kinase Homo sapiens 23-27 18429112-4 2001 These methods are followed by protocols for more comprehensive analysis of O-GlcNAc modified proteins, including labeling of O-GlcNAc residues with [3H]Gal, and subsequent product analysis. Tritium 149-151 O-linked N-acetylglucosamine (GlcNAc) transferase Homo sapiens 125-133 11591520-2 2001 ZD6021 inhibited binding of [3H]-NKA or [3H]-SP to human NK1 and NK2 receptors, with high-affinity (K(i)=0.12 and 0.62nM, respectively). Tritium 29-31 tachykinin receptor 1 Homo sapiens 57-60 11669456-2 2001 S2 hOAT1 and S2 hOAT3 exhibited a time- and dose-dependent, and a saturable increase in uptake of [3H]-OTA, with apparent Km values of 0.42 microM (hOAT1) and 0.75 microM (hOAT3). Tritium 99-101 solute carrier family 22 member 6 Homo sapiens 3-8 11669456-2 2001 S2 hOAT1 and S2 hOAT3 exhibited a time- and dose-dependent, and a saturable increase in uptake of [3H]-OTA, with apparent Km values of 0.42 microM (hOAT1) and 0.75 microM (hOAT3). Tritium 99-101 solute carrier family 22 member 8 Homo sapiens 16-21 11669456-2 2001 S2 hOAT1 and S2 hOAT3 exhibited a time- and dose-dependent, and a saturable increase in uptake of [3H]-OTA, with apparent Km values of 0.42 microM (hOAT1) and 0.75 microM (hOAT3). Tritium 99-101 solute carrier family 22 member 6 Homo sapiens 148-153 11669456-2 2001 S2 hOAT1 and S2 hOAT3 exhibited a time- and dose-dependent, and a saturable increase in uptake of [3H]-OTA, with apparent Km values of 0.42 microM (hOAT1) and 0.75 microM (hOAT3). Tritium 99-101 solute carrier family 22 member 8 Homo sapiens 172-177 11495721-7 2001 These enhancements induced by TNF-alpha and PDGF-BB might be due to an increase in BK B(2) receptor density (B(max)), since [3H]BK binding to TSMCs was inhibited by the B(2) selective agonist and antagonist, BK and Hoe 140, but not by the B(1) selective reagents. Tritium 125-127 tumor necrosis factor Canis lupus familiaris 30-39 11425508-4 2001 [3H]Ro 25-6981 (an NMDA antagonist selective for NR1/NR2B assembly) specific binding and mRNA levels of NMDA receptor subunits 1 and 2B decreased in CA1 after ovariectomy. Tritium 1-3 carbonic anhydrase 1 Rattus norvegicus 149-152 11396983-6 2001 No significant RyR1 fluorescence was observed at the plasma membrane.Fluo-4-loaded sf 21 cells expressing recombinant RyR1 responded to activating-low ryanodine concentrations (100 nM) or caffeine (10 mM) with a sharp rise in intracellular Ca2 followed by a sustained phase, in contrast, sf 21 cells expressing the human bradykinin type 2 receptor did not respond to ryanodine or caffeine.These results demonstrate the expression of recombinant RyR1 in sf 21 cells with functional properties similar to what has been previously reported for native RyR1 in mammalian tissues, however, some differences were observed in [3H]ryanodine binding assays compared to native rabbit RyR1. Tritium 619-621 ryanodine receptor 1 Oryctolagus cuniculus 118-122 11396983-6 2001 No significant RyR1 fluorescence was observed at the plasma membrane.Fluo-4-loaded sf 21 cells expressing recombinant RyR1 responded to activating-low ryanodine concentrations (100 nM) or caffeine (10 mM) with a sharp rise in intracellular Ca2 followed by a sustained phase, in contrast, sf 21 cells expressing the human bradykinin type 2 receptor did not respond to ryanodine or caffeine.These results demonstrate the expression of recombinant RyR1 in sf 21 cells with functional properties similar to what has been previously reported for native RyR1 in mammalian tissues, however, some differences were observed in [3H]ryanodine binding assays compared to native rabbit RyR1. Tritium 619-621 ryanodine receptor 1 Oryctolagus cuniculus 118-122 11396983-6 2001 No significant RyR1 fluorescence was observed at the plasma membrane.Fluo-4-loaded sf 21 cells expressing recombinant RyR1 responded to activating-low ryanodine concentrations (100 nM) or caffeine (10 mM) with a sharp rise in intracellular Ca2 followed by a sustained phase, in contrast, sf 21 cells expressing the human bradykinin type 2 receptor did not respond to ryanodine or caffeine.These results demonstrate the expression of recombinant RyR1 in sf 21 cells with functional properties similar to what has been previously reported for native RyR1 in mammalian tissues, however, some differences were observed in [3H]ryanodine binding assays compared to native rabbit RyR1. Tritium 619-621 ryanodine receptor 1 Oryctolagus cuniculus 118-122 11444589-7 2001 Following transfection of canine EP4 into CHO-KI cells, Scatchard analysis revealed a dissociation constant of 24 nM for PGE, while competition binding studies using 3H-PGE2 as ligand demonstrated specific displacement by PGE2 prostaglandin E, (PGE1), and prostaglandin A3 (PGA3). Tritium 166-168 prostaglandin E receptor 4 Homo sapiens 33-36 11453166-5 2001 An obvious difference between hippocampal slice cultures and hippocampi in situ was a changed distribution of binding sites among the hippocampal areas showing a relative increase of [3H]MK-801 and [3H]AMPA binding sites in CA3 as compared to CA1 and to the dentate gyrus in the cultures. Tritium 184-186 carbonic anhydrase 1 Rattus norvegicus 243-246 11453166-5 2001 An obvious difference between hippocampal slice cultures and hippocampi in situ was a changed distribution of binding sites among the hippocampal areas showing a relative increase of [3H]MK-801 and [3H]AMPA binding sites in CA3 as compared to CA1 and to the dentate gyrus in the cultures. Tritium 199-201 carbonic anhydrase 1 Rattus norvegicus 243-246 11426740-4 2001 T cell reactivity was greater against SAg (57%) than to Cp23 (34.3%) as measured by [3H]thymidine incorporation. Tritium 85-87 S-antigen visual arrestin Homo sapiens 38-41 11282082-7 2001 C2 caused up to 70% inhibition of BrdU and [3H]thymidine incorporation at non-toxic concentrations as a result of G1 cell-cycle arrest. Tritium 44-46 complement C2 Homo sapiens 0-2 11277567-4 2001 Orphanin FQ also inhibits [3H]dopamine uptake by rat striatal synaptosomes, which confirmed the effect of orphanin FQ on D8 cells. Tritium 27-29 prepronociceptin Rattus norvegicus 0-11 11073942-5 2001 Recombinant glutathione S-transferase fusion protein of Rag C efficiently bound to both [(3)H]GTP and [(3)H]GDP. Tritium 90-93 Ras related GTP binding C Homo sapiens 56-61 11305322-1 2001 PSGL-1, a specific ligand for P-, E- and L-selectin, was isolated from in vivo [3H]-glucosamine labeled HL-60 cells by a combination of wheat germ agglutinin-agarose and P- or E-selectin-agarose chromatography. Tritium 80-82 selectin P ligand Homo sapiens 0-6 11181015-5 2001 In isolated perfused hearts of aortic-banded rats, functional impairment of NET was indicated by a 37% reduction in [(3)H]-NE-uptake. Tritium 116-122 solute carrier family 6 member 2 Rattus norvegicus 76-79 11258963-2 2001 BPA competed with [3H]-17beta-estradiol (E2) for binding to mouse uterine cytosol ER, a glutathione S-transferase (GST)-human ER D, E, and F domain fusion protein (GST-hERalphadef) and full-length recombinant hERbeta. Tritium 19-21 hematopoietic prostaglandin D synthase Mus musculus 88-113 11258963-2 2001 BPA competed with [3H]-17beta-estradiol (E2) for binding to mouse uterine cytosol ER, a glutathione S-transferase (GST)-human ER D, E, and F domain fusion protein (GST-hERalphadef) and full-length recombinant hERbeta. Tritium 19-21 hematopoietic prostaglandin D synthase Mus musculus 115-118 11811668-5 2001 The muscarinic receptor-mediated [3H]CDP-DAG response was significantly blunted in the aged rats in subiculum, CA3, and CA1. Tritium 34-36 carbonic anhydrase 1 Rattus norvegicus 120-123 10913689-5 2001 Increases in [3H]nisoxetine binding were found in the cingulate, frontal, parietal, agranular insular, entorhinal and perirhinal cortices as well as in the hippocampal divisions CA1, CA3, dentate gyrus and subiculum, and in nucleus raphe dorsalis of trimipramine-treated animals compared to the control rats. Tritium 14-16 carbonic anhydrase 1 Rattus norvegicus 178-181 11281366-7 2000 The effect of leptin on proliferation and viability of adrenal cells in primary culture and of the NCI-H295 cell line was studied by the WST-1 assay and by 3H-thymidine test. Tritium 156-158 leptin Homo sapiens 14-20 11138846-0 2000 [3H]ac-RYYRWK-NH2, a novel specific radioligand for the nociceptin/orphanin FQ receptor. Tritium 1-3 prepronociceptin Rattus norvegicus 56-66 11208181-7 2000 The latency and bioactivity of TGF-beta1 released from pLin-TGF-beta1 transfected cells were confirmed by evaluation of 3H-thymidine incorporation in Mv1Lu epithelial cells treated with non- and heat-activated 10% conditioned medium. Tritium 120-122 perilipin 1 Mus musculus 55-59 11012835-5 2000 bFGF was also the most active at 3-DIV, and IGF-I at 8-DIV, in counteracting SD-induced cell death, whereas EGF was the most potent in increasing [3H]thymidine incorporation. Tritium 147-149 epidermal growth factor Mus musculus 108-111 10953996-8 2000 RESULTS: Both IGF-I and insulin increased 3H-TdR uptake by cultured rat VSMC (P < 0.05). Tritium 42-44 insulin-like growth factor 1 Rattus norvegicus 14-19 10942233-10 2000 Further, the % inhibition of 3H-thymidine uptake of cell lines positively correlated with the amount of their intracellular G-CSF measured by enzyme immunoassay, suggesting an autocrine growth mechanism via the G-CSF/G-CSFR interaction. Tritium 29-31 colony stimulating factor 3 Homo sapiens 124-129 10942233-10 2000 Further, the % inhibition of 3H-thymidine uptake of cell lines positively correlated with the amount of their intracellular G-CSF measured by enzyme immunoassay, suggesting an autocrine growth mechanism via the G-CSF/G-CSFR interaction. Tritium 29-31 colony stimulating factor 3 Homo sapiens 211-216 10942233-10 2000 Further, the % inhibition of 3H-thymidine uptake of cell lines positively correlated with the amount of their intracellular G-CSF measured by enzyme immunoassay, suggesting an autocrine growth mechanism via the G-CSF/G-CSFR interaction. Tritium 29-31 colony stimulating factor 3 receptor Homo sapiens 217-223 10944004-6 2000 Antagonists of GLURs and the polyamine site inhibited L-glutamate evoked [3H]D-aspartate overflow with the following rank order of potency: MCPG >ifenprodil > AP-5 > arcaine> MK-801. Tritium 74-76 glutamyl-tRNA synthetase 2, mitochondrial Homo sapiens 15-20 11314343-9 2000 FN (20, 40 micrograms/ml) stimulated cell proliferation, promoted incorporation of [3H]-thymidine, and increased the levels of ALPase activity in supernatant. Tritium 84-86 fibronectin 1 Bos taurus 0-2 10779365-2 2000 The phorbol ester [(3)H]phorbol 12, 13-dibutyrate ([(3)H]PDBu) bound to this C1 domain (C1-RasGRP) with a dissociation constant of 0.58 +/- 0.08 nM, similar to that observed previously for PKC. Tritium 18-23 RAS guanyl releasing protein 1 Homo sapiens 91-97 10781927-8 2000 OX-LDL-induced [3H]thymidine incorporation and p42/p44 MAPK phosphorylation was completely inhibited by PD98059 (an inhibitor of MEK1/2) and SB203580 (an inhibitor of p38 MAPK). Tritium 16-18 dual specificity mitogen-activated protein kinase kinase 2 Canis lupus familiaris 129-135 10822009-2 2000 Treatment of Ishikawa cells with the AhR agonist [3H]2,3,7, 8-tetrachlorodibenzo-p-dioxin (TCDD) gave a radiolabeled nuclear complex that sedimented at 6.0 S in sucrose density gradients, and Western blot analysis confirmed that Ishikawa cells expressed human AhR and AhR nuclear translocator (Arnt) proteins. Tritium 50-52 aryl hydrocarbon receptor nuclear translocator Homo sapiens 268-292 10822009-2 2000 Treatment of Ishikawa cells with the AhR agonist [3H]2,3,7, 8-tetrachlorodibenzo-p-dioxin (TCDD) gave a radiolabeled nuclear complex that sedimented at 6.0 S in sucrose density gradients, and Western blot analysis confirmed that Ishikawa cells expressed human AhR and AhR nuclear translocator (Arnt) proteins. Tritium 50-52 aryl hydrocarbon receptor nuclear translocator Homo sapiens 294-298 10720031-4 2000 Treatment for 24 h with activin (0-10 ng/mL) significantly inhibited cell proliferation in 5 tumors (P < 0.05), as determined by [3H]thymidine incorporation. Tritium 133-135 inhibin subunit beta E Homo sapiens 24-31 10746941-3 2000 Initially, test chemicals were screened at high concentrations to determine whether a chemical competed with [3H]-estradiol for the ER. Tritium 110-112 estrogen receptor 1 Rattus norvegicus 132-134 10646530-3 2000 Western blot analysis showed that treatment of SH-SY5Y cells for 72 h with the cholinesterase inhibitors tacrine (10(-5) M), donepezil (10(-5) M), and galanthamine (10(-5) M), nicotine (10(-5) M), and epibatidine (10(-7) M) increased tau levels as detected with Tau-1, AT 8, and AT 270 monoclonal antibodies and binding of [3H]epibatidine. Tritium 324-326 butyrylcholinesterase Homo sapiens 79-93 10600650-6 2000 [(3)H]Palmitate incorporation into unbound SNAP-25 was negligible, but was stimulated 100-fold when SNAP-25 was present in the SNARE complex. Tritium 0-5 synaptosome associated protein 25 Homo sapiens 43-50 10761690-11 2000 DNA synthesis evaluated by [3H]-thymidine incorporation was found to be increased by 45.2 +/- 5.6% in response to EGF (10(-8) M) and decreased by 11.7 +/- 2.6% to octreotide (10(-8) M) compared to controls (P < 0.01). Tritium 28-30 epidermal growth factor Homo sapiens 114-117 10761690-12 2000 The increase in [3H]-thymidine incorporation was significantly lower when EGF treatment was combined with octreotide administration (10.1 +/- 2.5% over control). Tritium 17-19 epidermal growth factor Homo sapiens 74-77 10674003-4 1999 Cellular responses were also related to thymidylate synthase topoisomerase I and carboxyl esterase activities, which were assessed by a ligand-binding and a 3H-release assay; a DNA decatenation assay; and a spectrophotometric method, respectively. Tritium 157-159 thymidylate synthetase Homo sapiens 40-60 10608276-0 1999 [3H]ATPA: a high affinity ligand for GluR5 kainate receptors. Tritium 1-3 glutamate ionotropic receptor kainate type subunit 1 Rattus norvegicus 37-42 10598792-7 1999 The EC50 for the accumulation of [3H]IP1, the major product detected (85+/-2% of total [3H]IP1 + [3H]IP2 + [3H]IP3), was 0.38+/-0.05 microM. Tritium 34-36 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 37-40 10598792-7 1999 The EC50 for the accumulation of [3H]IP1, the major product detected (85+/-2% of total [3H]IP1 + [3H]IP2 + [3H]IP3), was 0.38+/-0.05 microM. Tritium 34-36 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 91-94 10598792-7 1999 The EC50 for the accumulation of [3H]IP1, the major product detected (85+/-2% of total [3H]IP1 + [3H]IP2 + [3H]IP3), was 0.38+/-0.05 microM. Tritium 34-36 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 101-104 10598792-7 1999 The EC50 for the accumulation of [3H]IP1, the major product detected (85+/-2% of total [3H]IP1 + [3H]IP2 + [3H]IP3), was 0.38+/-0.05 microM. Tritium 88-90 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 37-40 10598792-7 1999 The EC50 for the accumulation of [3H]IP1, the major product detected (85+/-2% of total [3H]IP1 + [3H]IP2 + [3H]IP3), was 0.38+/-0.05 microM. Tritium 88-90 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 91-94 10598792-7 1999 The EC50 for the accumulation of [3H]IP1, the major product detected (85+/-2% of total [3H]IP1 + [3H]IP2 + [3H]IP3), was 0.38+/-0.05 microM. Tritium 88-90 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 37-40 10598792-7 1999 The EC50 for the accumulation of [3H]IP1, the major product detected (85+/-2% of total [3H]IP1 + [3H]IP2 + [3H]IP3), was 0.38+/-0.05 microM. Tritium 88-90 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 91-94 10598792-7 1999 The EC50 for the accumulation of [3H]IP1, the major product detected (85+/-2% of total [3H]IP1 + [3H]IP2 + [3H]IP3), was 0.38+/-0.05 microM. Tritium 88-90 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 37-40 10598792-7 1999 The EC50 for the accumulation of [3H]IP1, the major product detected (85+/-2% of total [3H]IP1 + [3H]IP2 + [3H]IP3), was 0.38+/-0.05 microM. Tritium 88-90 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 91-94 10598792-8 1999 Noradrenaline-induced [3H]IP1 accumulation was also inhibited by alpha1-antagonists. Tritium 23-25 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 26-29 10598792-12 1999 Pretreatment with chloroethylclonidine (10 microM, 15 min) abolished noradrenaline-induced [3H]IP1 accumulation and Ca2+ mobilisation. Tritium 92-94 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 95-98 10509644-12 1999 Exposure of cells to serum plus TGF-beta2 suppressed [3H]thymidine incorporation in a dose-dependent manner at concentrations ranging from 5 pg/ml to 5 ng/ml. Tritium 54-56 transforming growth factor, beta 2 Rattus norvegicus 32-41 10087334-10 1999 The maximum binding capacity (Bmax) of the high-affinity radioligand [3H]PN200-110 in control and IGF-1-treated cells was 1.21 +/- 0.25 and 3.15 +/- 0.5 pmol (mg protein)-1, respectively (P < 0.01). Tritium 70-72 insulin-like growth factor 1 Rattus norvegicus 98-103 10232825-8 1999 TS-tris administration protected against CCl4-induced microsomal lipid peroxide formation and inactivation of the microsomal enzyme glucose-6-phosphatase (G6Pase). Tritium 0-3 glucose-6-phosphatase catalytic subunit 1 Rattus norvegicus 132-153 10232825-8 1999 TS-tris administration protected against CCl4-induced microsomal lipid peroxide formation and inactivation of the microsomal enzyme glucose-6-phosphatase (G6Pase). Tritium 0-3 glucose-6-phosphatase catalytic subunit 1 Rattus norvegicus 155-161 10191285-8 1999 Uptake of exogenous [3H]cholesterol and total cholesterol mass were increased 1.9- and 1.4-fold, respectively, in SCP-x expressors. Tritium 21-23 sterol carrier protein 2, liver Mus musculus 114-119 10221754-0 1999 Acetylcholinesterase potentiates [3H]fluorowillardiine and [3H]AMPA binding to rat cortical membranes. Tritium 34-36 acetylcholinesterase Rattus norvegicus 0-20 10221754-2 1999 We have investigated the effects of AChE on the binding of the specific AMPA receptor agonists (S)-[3H]5-fluorowillardiine ([3H]FW) and [3H]AMPA to rat cortical membranes. Tritium 100-102 acetylcholinesterase Rattus norvegicus 36-40 10221754-6 1999 [3H]FW binding to membranes from young (15-day-old) rats is four orders of magnitude more sensitive to AChE modulation than membranes from adult rats (EC50 values of 4x10(-5) and 0.1 unit/ml, respectively) although the total percentage increase in binding is similar. Tritium 1-3 acetylcholinesterase Rattus norvegicus 103-107 10221754-7 1999 Furthermore, the AChE-induced potentiation of [3H]FW binding is Ca2+ - and temperature-dependent suggesting an enzymatic action for AChE in this system. Tritium 47-49 acetylcholinesterase Rattus norvegicus 17-21 10221754-7 1999 Furthermore, the AChE-induced potentiation of [3H]FW binding is Ca2+ - and temperature-dependent suggesting an enzymatic action for AChE in this system. Tritium 47-49 acetylcholinesterase Rattus norvegicus 132-136 10221754-8 1999 Saturation binding experiments with [3H]FW to adult membranes reveal high and low affinity binding sites and demonstrate that the main action of AChE is to increase the Bmax of both sites. Tritium 37-39 acetylcholinesterase Rattus norvegicus 145-149 10404512-7 1999 Glycine-extended gastrin induced a dose-dependent increase in [3H]thymidine uptake in LoVo (143 +/- 8% versus control at 10(-10) M) and HT 29 (151 +/- 11% versus control at 10(-10) M) cells that was not inhibited by PD 134308 or by a mitogen-activated protein (MAP) or ERK kinase (MEK) inhibitor (PD 98509). Tritium 63-65 gastrin Homo sapiens 17-24 10022505-8 1999 Enforced ectopic expression of Mad1 in the cells results in inhibition of [3H]thymidine incorporation and proliferation in response to CSF-1. Tritium 75-77 colony stimulating factor 1 Homo sapiens 135-140 9930760-6 1999 In these regions, mGluR8 is likely to be labeled by [3H]L-AP4 because mGluR8 is expressed in such brain regions and, like mGluR4, has high affinity for L-AP4. Tritium 53-55 glutamate receptor, metabotropic 8 Mus musculus 18-24 9930760-6 1999 In these regions, mGluR8 is likely to be labeled by [3H]L-AP4 because mGluR8 is expressed in such brain regions and, like mGluR4, has high affinity for L-AP4. Tritium 53-55 glutamate receptor, metabotropic 8 Mus musculus 70-76 9989292-10 1999 The iPLA2, not the calcium-dependent PLA2 (cPLA2), released [3H]oleic acid ([3H]OA) from RSMG ductal cells. Tritium 61-63 phospholipase A2 group VI Rattus norvegicus 4-9 9886823-4 1999 Receptor binding assays on membrane preparations from GT1-1 cells showed the presence of a single specific and saturable class of binding sites (Kd = 4.6 nM; 10,000 sites/cell) for [3H]iloprost, a stable analog of PGI2. Tritium 182-184 solute carrier family 2 (facilitated glucose transporter), member 1 Mus musculus 54-59 10907726-5 1999 In MPTP-treated marmosets BDNF caused increased ipsilateral striatal [3H]mazindol binding with increased somatic size and staining intensity in GAD-immunoreactive cells and a 10-20% loss of nigral TH-immunoreactive cells with increased GFAP staining. Tritium 70-72 brain-derived neurotrophic factor Callithrix jacchus 26-30 9830012-5 1998 ShK-Dap22, at subnanomolar concentrations, suppressed anti-CD3 induced human T-lymphocyte [3H]thymidine incorporation in vitro. Tritium 91-93 sedoheptulokinase Homo sapiens 0-3 9828171-11 1998 Addition of IGF-I or insulin to the culture medium of UV-irradiated embryos improved [3H]thymidine incorporation and blastocyst formation significantly. Tritium 86-88 insulin-like growth factor I Oryctolagus cuniculus 12-17 9836514-6 1998 Moreover, radiolabeled [3H]KRP-297 bound directly to PPAR-alpha and PPAR-gamma with dissociation constants of 228 and 326 nmol/l, respectively. Tritium 24-26 peroxisome proliferator activated receptor alpha Rattus norvegicus 53-63 9815276-0 1998 In vivo dose-related receptor binding of the vitamin D analogue [3H]-1,25-dihydroxy-22-oxavitamin D3 (OCT) in rat parathyroid, kidney distal and proximal tubules, duodenum, and skin, studied by quantitative receptor autoradiography. Tritium 65-67 plexin A2 Homo sapiens 102-105 9832151-2 1998 The glycosylation inhibitor tunicamycin induced a dose-dependent decrease in the rVMAT1-mediated uptake of [3H]serotonin. Tritium 107-111 solute carrier family 18 member A1 Rattus norvegicus 81-87 9990545-0 1998 Characterization of the high affinity [3H]nociceptin binding site in membrane preparations of rat heart: correlations with the non-opioid dynorphin binding site. Tritium 39-41 prepronociceptin Rattus norvegicus 42-52 9990545-1 1998 The binding parameters of [3H]nociceptin were examined in membrane preparations of rat heart and compared with those of [3H]dynorphin A-(1-13) ([3H]Dyn A-(1-13)). Tritium 27-29 prepronociceptin Rattus norvegicus 30-40 9990545-2 1998 Scatchard analysis of [3H]nociceptin binding revealed the presence of two distinct sites: a high affinity (Kd: 583 nM) low capacity (Bmax: 132 pmol/mg protein) site and a low affinity (Kd: 10,316 nM) high capacity (1552 pmol/mg protein) site. Tritium 23-25 prepronociceptin Rattus norvegicus 26-36 9990545-5 1998 The order of potency of the various peptides in inhibiting [3H]nociceptin binding correlated well (r = 0.93) with their ability to complete with the binding of [3H]Dyn A-(1-13) (Dumont and Lemaire, 1993). Tritium 60-62 prepronociceptin Rattus norvegicus 63-73 9990545-5 1998 The order of potency of the various peptides in inhibiting [3H]nociceptin binding correlated well (r = 0.93) with their ability to complete with the binding of [3H]Dyn A-(1-13) (Dumont and Lemaire, 1993). Tritium 161-163 prepronociceptin Rattus norvegicus 63-73 9990545-6 1998 In addition, the high affinity [3H]nociceptin and non-opioid [3H]Dyn A-(1-13) sites were both sensitive to NaCl (120 mM) and the phospholipase C (PLC) inhibitors, U-73122 and neomycin (100 microM). Tritium 32-34 prepronociceptin Rattus norvegicus 35-45 9990545-9 1998 Nociceptin (1-50 microM) was also shown to inhibit the uptake of [3H]noradrenaline ([3H]NA) by cardiac synaptosomal preparations. Tritium 66-68 prepronociceptin Rattus norvegicus 0-10 9990545-10 1998 In spontaneously hypertensive rats (SHR), the potency of nociceptin in inhibiting [3H]NA uptake was increased by 1.6-fold as compared with Wistar Kyoto (WKY) control rats and such effect was accompanied by comparable increased levels of cardiac ORL1 mRNA and [3H]nociceptin high affinity sites. Tritium 83-85 prepronociceptin Rattus norvegicus 57-67 9990545-10 1998 In spontaneously hypertensive rats (SHR), the potency of nociceptin in inhibiting [3H]NA uptake was increased by 1.6-fold as compared with Wistar Kyoto (WKY) control rats and such effect was accompanied by comparable increased levels of cardiac ORL1 mRNA and [3H]nociceptin high affinity sites. Tritium 260-262 prepronociceptin Rattus norvegicus 57-67 9990545-12 1998 The results demonstrate that in rat heart the characteristics of the high affinity, low capacity [3H]nociceptin binding site are similar to those of the non-opioid Dyn binding site. Tritium 98-100 prepronociceptin Rattus norvegicus 101-111 9990545-13 1998 The stimulation of this site by nociceptin, Dyn A or related peptides is more likely to produce a modulation of PLC activity and [3H]NA uptake and may participate to the pathophysiology of hypertension. Tritium 130-132 prepronociceptin Rattus norvegicus 32-42 10344792-0 1998 Recombinant caprine 3H-[N-acetylglucosamine-6-sulfatase] and human 3H-[N-acetylgalactosamine-4-sulfatase]: plasma clearance, tissue distribution, and cellular uptake in the rat. Tritium 67-69 arylsulfatase B Homo sapiens 71-104 9864269-2 1998 Both native and the synthetic Pardaxin selectively stimulated phospholipase A2 (PLA2) activity (measured by [3H]AA release) in the presence as well as in the absence of extracellular calcium. Tritium 109-111 phospholipase A2 group IB Rattus norvegicus 62-78 9864269-2 1998 Both native and the synthetic Pardaxin selectively stimulated phospholipase A2 (PLA2) activity (measured by [3H]AA release) in the presence as well as in the absence of extracellular calcium. Tritium 109-111 phospholipase A2 group IB Rattus norvegicus 80-84 9822642-6 1998 Base-line and bradykinin-induced [3H]AA release were suppressed by arachidonyltrifluoromethyl ketone, a relatively specific inhibitor of cytosolic phospholipase A2 at 40 microM, and simultaneous administration of coupling factor 6 showed no further effect. Tritium 34-36 phospholipase A2 group IVA Rattus norvegicus 137-163 9822642-6 1998 Base-line and bradykinin-induced [3H]AA release were suppressed by arachidonyltrifluoromethyl ketone, a relatively specific inhibitor of cytosolic phospholipase A2 at 40 microM, and simultaneous administration of coupling factor 6 showed no further effect. Tritium 34-36 ATP synthase peripheral stalk subunit F6 Rattus norvegicus 213-230 9851589-0 1998 Receptor binding characteristics of [3H]NAD-299, a new selective 5-HT1A receptor antagonist. Tritium 37-39 5-hydroxytryptamine receptor 1A Homo sapiens 65-80 9851589-5 1998 The rank order of potency of various compounds to inhibit [3H]NAD-299 binding is consistent with the labeling of 5-HT1A receptors. Tritium 59-61 5-hydroxytryptamine receptor 1A Homo sapiens 113-119 9879986-6 1998 Estrogen receptor (ER) bound [3H]-E2 was displaced by E2 and MENT with ED50 values of 70 pg and 250 ng, respectively, a 3,500 fold difference in their binding affinity. Tritium 30-32 estrogen receptor 1 Rattus norvegicus 0-17 9879986-6 1998 Estrogen receptor (ER) bound [3H]-E2 was displaced by E2 and MENT with ED50 values of 70 pg and 250 ng, respectively, a 3,500 fold difference in their binding affinity. Tritium 30-32 estrogen receptor 1 Rattus norvegicus 19-21 9761715-3 1998 We have confirmed these findings and employed [3H]palmitate labelling of PC12 cells to demonstrate that Csp1 remains associated with membranes following the complete removal of palmitic acid residues. Tritium 47-49 common salivary protein 1 Rattus norvegicus 104-108 9776313-2 1998 SK&F 96365-induced accumulation of [3H]IP1 increased linearly with time, but there was no initial rapid formation of [3H]IP3. Tritium 40-42 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 43-46 9776313-3 1998 SK&F 96365 also stimulated [3H]IP1 accumulation in human HeLa cells, but only to a small extent in slices of rat cerebral cortex and guinea-pig cerebellum. Tritium 32-34 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 35-38 9776313-4 1998 SK&F 96365-induced accumulation of [3H]IP1 in U373 MG cells increased as extracellular Ca2+ was increased from nominally zero to 4 mM, but there was no evidence that SK&F 96365 induced any marked entry of Ca2+ into cells; only an inhibition of store-refilling-induced Ca2+ entry was apparent. Tritium 40-42 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 43-46 10529142-3 1999 The proliferative response of mononuclear cells (MNC) to mycobacterial 65 kDa HSP (mHSP 65) was measured by [3H] thymidine incorporation. Tritium 109-111 heat shock protein 1 (chaperonin) Mus musculus 83-90 10465261-5 1999 Treatment with estradiol (48 h) stimulated progesterone receptor (PR) messenger RNA expression and binding to [3H]R5020, a synthetic progestin. Tritium 111-113 progesterone receptor Rattus norvegicus 43-64 10465261-5 1999 Treatment with estradiol (48 h) stimulated progesterone receptor (PR) messenger RNA expression and binding to [3H]R5020, a synthetic progestin. Tritium 111-113 progesterone receptor Rattus norvegicus 66-68 10403380-3 1999 Transfection of cPLA2 into sPLA2-IIA-expressing cells produced a synergistic increase in IL-1-dependent [3H]AA release and subsequent prostaglandin production. Tritium 105-107 interleukin 1 alpha Homo sapiens 89-93 10340626-6 1999 An extensive decrease of [3H]GBR12935 DAT binding sites (-92% vs. Control) was observed in the striatum of MPTP-monkeys and an important loss of DAT mRNA(-86% vs. Control) was observed in substantia nigra. Tritium 26-28 sodium-dependent dopamine transporter Macaca fascicularis 38-41 10338149-2 1999 We showed that zinc chelation by DTPA (diethylenetriaminepenta-acetic acid) inhibits [3H]thymidine incorporation stimulated by IGF-I in Rat-1 fibroblasts. Tritium 86-88 insulin-like growth factor 1 Rattus norvegicus 127-132 10338149-4 1999 The stimulation of MAPK, which is crucial for the [3H]thymidine incorporation induced by IGF-I in Rat-1 cells, was partially blunted by DTPA. Tritium 51-53 insulin-like growth factor 1 Rattus norvegicus 89-94 10191274-6 1999 The monomeric peptides partly inhibit the release of [3H]5-HT by CRP, suggesting that they are partial agonists of the collagen receptor GPVI. Tritium 54-56 glycoprotein VI platelet Homo sapiens 137-141 10098512-4 1999 To address this question, we first assessed the direct GRF effect on second messenger activation in cultures of LD and HD cells by measuring cAMP levels and [3H]myo-inositol incorporation. Tritium 158-160 growth hormone releasing hormone Sus scrofa 55-58 10382942-4 1999 The addition of IL-6 or both IL-6 and MGF to M-CSF containing cultures resulted in significant higher numbers of colony-forming unit-macrophage (CFU-M) as tested in clonogenic and 3H-thymidine assays. Tritium 180-182 interleukin 3 Homo sapiens 38-41 9757030-1 1998 Following pulse labeling with [3H]arachidonic acid ([3H]AA), its incorporation pattern in brain reflects regional changes in neurotransmitter signal transduction using phospholipase A2, that is, functional activity. Tritium 31-33 phospholipase A2 group IB Rattus norvegicus 168-184 9757064-10 1998 However, the benzodiazepine agonist-insensitive subtype of [3H]Ro15-4513 binding activity, a pharmacological motif of alpha6 subunit-containing GABAA receptors, was lower in stg/stg mice relative to the +/+ strain which correlated with the lowered level of alpha6 subunit expression. Tritium 60-62 gamma-aminobutyric acid (GABA) A receptor, subunit alpha 6 Mus musculus 118-124 9806343-3 1998 SUR2B, a proposed subunit of the vascular KATP channel, was expressed in HEK 293 cells and binding assays were performed in membranes at 37 degrees C using the tritiated KATP channel opener, [3H]-P1075. Tritium 192-194 ATP-binding cassette, sub-family C (CFTR/MRP), member 9 Mus musculus 0-5 10382942-4 1999 The addition of IL-6 or both IL-6 and MGF to M-CSF containing cultures resulted in significant higher numbers of colony-forming unit-macrophage (CFU-M) as tested in clonogenic and 3H-thymidine assays. Tritium 180-182 colony stimulating factor 1 Homo sapiens 45-50 2314388-9 1990 Among the various drugs tested, only inhibitors of MAO-A were able to effectively prevent [3H]Ro 41-1049 specific binding. Tritium 91-93 monoamine oxidase A Homo sapiens 51-56 2314388-14 1990 The presence of MAO-A- but not MAO-B-selective inhibitors prevented the covalent incorporation of [3H]Ro 41-1049. Tritium 99-101 monoamine oxidase A Homo sapiens 16-21 10037455-5 1999 Administration of EGF delayed H2O2 and XO + X-induced changes in TER, dilution potential, and [3H]mannitol flux. Tritium 95-97 epidermal growth factor Homo sapiens 18-21 9804290-3 1998 caused significant decreases in [3H]MK801 binding in several hippocampus subareas and layers, mainly in CA1 and CA3 at seizure (11-27%) and postseizure (8-16%) and in cerebral occipital cortex at seizure (18-22%). Tritium 33-35 carbonic anhydrase 1 Rattus norvegicus 104-107 2314388-15 1990 The present results indicate that [3H]Ro 41-1049 is incorporated into a subunit of MAO-A, in the presence of NaBH3CN, and modifies a protein domain that is essential for the enzyme activity. Tritium 35-37 monoamine oxidase A Homo sapiens 83-88 9804290-7 1998 When CPA was administered 30 minutes before MP (which delayed seizure onset) and rats were sacrificed at seizure, decreases in [3H]MK801 binding in several layers of CA1 and CA3 of hippocampus (11-27%) and in CA1, CA2, CA3 (24-35%) after CPA+MP postseizure, and an increase in CA2 after CPA and CPA+MP postseizure (20-34%), were observed. Tritium 128-130 carbonic anhydrase 1 Rattus norvegicus 166-169 2305876-3 1990 Histomorphometric analysis of caudal vertebrae showed that EGF (20 and 200 ng.g-1.day-1) reduced the endosteal matrix and mineral appositional rates after 5 days of treatment as measured by double [3H]proline labeling and double tetracycline labeling, respectively. Tritium 198-200 epidermal growth factor Mus musculus 59-62 9690202-6 1998 Overexpression of either cPLA2-wt or cPLA2-SA505 markedly amplified the release of [3H]AA by C5b-9. Tritium 84-86 phospholipase A2 group IVA Rattus norvegicus 25-30 9690202-6 1998 Overexpression of either cPLA2-wt or cPLA2-SA505 markedly amplified the release of [3H]AA by C5b-9. Tritium 84-86 phospholipase A2 group IVA Rattus norvegicus 37-42 10096558-10 1999 Flux through thymidylate synthase, as measured by 3H release from [3H]dUrd, was inhibited by OGT 719 at 4 h. The notable difference in the potency of OGT 719 efficacy on C170HM2 cells in vitro and in vivo supports our model of liver-specific activation of OGT 719. Tritium 50-52 thymidylate synthetase Homo sapiens 13-33 10096558-10 1999 Flux through thymidylate synthase, as measured by 3H release from [3H]dUrd, was inhibited by OGT 719 at 4 h. The notable difference in the potency of OGT 719 efficacy on C170HM2 cells in vitro and in vivo supports our model of liver-specific activation of OGT 719. Tritium 50-52 O-linked N-acetylglucosamine (GlcNAc) transferase Homo sapiens 93-96 10096558-10 1999 Flux through thymidylate synthase, as measured by 3H release from [3H]dUrd, was inhibited by OGT 719 at 4 h. The notable difference in the potency of OGT 719 efficacy on C170HM2 cells in vitro and in vivo supports our model of liver-specific activation of OGT 719. Tritium 67-69 thymidylate synthetase Homo sapiens 13-33 10064745-5 1999 The tissue sites of uptake of 3H label by wild type mice and LpL-null mice overexpressing human LpL in muscle indicate that LpL expression does influence accumulation of chylomicron retinoid. Tritium 30-32 lipoprotein lipase Homo sapiens 96-99 10064745-5 1999 The tissue sites of uptake of 3H label by wild type mice and LpL-null mice overexpressing human LpL in muscle indicate that LpL expression does influence accumulation of chylomicron retinoid. Tritium 30-32 lipoprotein lipase Homo sapiens 96-99 10064745-6 1999 Skeletal muscle from mice overexpressing human LpL accumulated 1.7- to 2.4-fold more 3H label than wild type. Tritium 85-87 lipoprotein lipase Homo sapiens 47-50 10064745-7 1999 Moreover, heart tissue from mice overexpresssing human LpL, but lacking mouse LpL, accumulated less than half of the 3H-label taken up by wild type heart. Tritium 117-119 lipoprotein lipase Homo sapiens 55-58 10188977-2 1999 Binding assays with membranes from CHO cells stably transfected with human CB1 or CB2 receptors using [3H]-CP55940, confirmed the CB2-selectivity of L759633 and L759656 (CB2/CB1 affinity ratios = 163 and 414 respectively) and showed AM630 to have a Ki at CB2 receptors of 31.2 nM and a CB2/CB1 affinity ratio of 165. Tritium 103-105 cannabinoid receptor 2 Homo sapiens 82-85 9671522-1 1998 Purified butyrylcholinesterase (BuChE) was photolabeled by [3H]-p-N, N-dimethylamino benzene diazonium ([3H]DDF) to identify the quaternary ammonium binding sites on this protein [Ehret-Sabatier, L. , Schalk, I., Goeldner, M., and Hirth, C. (1992) Eur. Tritium 60-62 butyrylcholinesterase Homo sapiens 9-30 9671522-1 1998 Purified butyrylcholinesterase (BuChE) was photolabeled by [3H]-p-N, N-dimethylamino benzene diazonium ([3H]DDF) to identify the quaternary ammonium binding sites on this protein [Ehret-Sabatier, L. , Schalk, I., Goeldner, M., and Hirth, C. (1992) Eur. Tritium 60-62 butyrylcholinesterase Homo sapiens 32-37 9671523-5 1998 P-selectin affinity chromatography of lysates from in vivo [3H]-glucosamine-labeled NKI-4 cells resulted in the isolation of three glycoproteins, with apparent molecular masses of approximately 250, approximately 110, and approximately 100 kDa under reducing conditions and approximately 230, approximately 105, and approximately 85 kDa under nonreducing conditions. Tritium 60-62 selectin P Homo sapiens 0-10 9879066-8 1998 In agreement pertussis toxin was found to reduce high affinity [3H]-NPY binding sites in CHO-Y1 cells whereas [3H]-BIBP3226 binding parameters remained unchanged. Tritium 64-66 neuropeptide Y Homo sapiens 68-71 2106323-3 1990 In the present study it is shown that addition of zinc chloride (40 microM) to primary mouse hepatocytes, cultured in Dulbecco"s minimal essential medium, markedly enhanced growth factor (EGF)-stimulated [3H]thymidine incorporation into DNA. Tritium 205-207 epidermal growth factor Mus musculus 188-191 9699220-2 1998 [3H]SCH 23390, [3H]spiperone, and [3H]-8-OH-DPAT were employed as specific radioligands for the D1, D2 and 5-HT1A receptors, respectively. Tritium 1-3 5-hydroxytryptamine receptor 1A Bos taurus 107-113 9674584-3 1998 Analysis by in vitro autoradiography showed a significant decrease in [3H]MK-801 binding in the dentate gyrus, CA1 and CA4 areas, as well as the temporal cortex. Tritium 71-73 carbonic anhydrase 1 Rattus norvegicus 111-114 9931444-3 1999 [3H]apoB, isolated from [3H]leucine pulsed/chased Hep G2 cells, was degraded 51% when incubated for 2 h at 37 degreesC in an assay mixture that included RR lysate (source of the ubiquitin conjugation system and proteasome) and an exogenous ATP regenerating system. Tritium 1-3 apolipoprotein B Oryctolagus cuniculus 4-8 9931444-3 1999 [3H]apoB, isolated from [3H]leucine pulsed/chased Hep G2 cells, was degraded 51% when incubated for 2 h at 37 degreesC in an assay mixture that included RR lysate (source of the ubiquitin conjugation system and proteasome) and an exogenous ATP regenerating system. Tritium 1-3 ubiquitin Oryctolagus cuniculus 178-187 9931444-3 1999 [3H]apoB, isolated from [3H]leucine pulsed/chased Hep G2 cells, was degraded 51% when incubated for 2 h at 37 degreesC in an assay mixture that included RR lysate (source of the ubiquitin conjugation system and proteasome) and an exogenous ATP regenerating system. Tritium 25-27 apolipoprotein B Oryctolagus cuniculus 4-8 9878841-4 1999 These sizable genotypic differences in analgesic potency were accompanied by HA/LA and HAR/LAR differences in whole-brain homogenate [3H]DPDPE and/or [3H]DELT, but paradoxically not [3H]DAMGO, binding. Tritium 151-153 low antibody response Mus musculus 91-94 1967632-2 1990 [3H]CNQX binding was regionally distributed, with the highest levels of binding present in hippocampus in the stratum radiatum of CA1, stratum lucidum of CA3, and molecular layer of dentate gyrus. Tritium 1-3 carbonic anhydrase 1 Rattus norvegicus 130-133 10698291-2 1999 With [5-(3)H]dUTP used as the substrate, dUTPase, converts it to the corresponding monophosphate and is coupled with thymidylate synthase-catalyzed reaction, resulting in tritium release from [5-(3)H]dUMP. Tritium 171-178 thymidylate synthetase Homo sapiens 117-137 9698216-7 1998 Because agonist binding requires coupling to G proteins, the present results suggest that the rapid over-expression of Galphai1/2 proteins induced by EEDQ is related to the biphasic recovery of [3H]UK 14304 binding. Tritium 195-197 G protein subunit alpha i2 Rattus norvegicus 119-129 9581681-12 1998 Addition of 100 nmol/L IGF-I stimulated cell proliferation of early cultured HSCs 5.6+/-1.1- and 4.6+/-0.2-fold as measured by [3H]thymidine and 5-bromo-2"-deoxyuridine incorporation, respectively. Tritium 128-130 insulin-like growth factor 1 Rattus norvegicus 23-28 9892181-1 1999 The study on incorporation of [3H](-)-epigallocatechin gallate (EGCG) into human lung cancer cell line PC-9 indicated that the [3H]EGCG incorporation was significantly enhanced by (-)-epicatechin, an inert tea polyphenol without a galloyl moiety. Tritium 30-33 proprotein convertase subtilisin/kexin type 9 Homo sapiens 103-107 2154668-5 1990 A comparison analysis of the binding data with the secondary structure characteristics of the peptides demonstrated that TTN (DBI 17-50) and DBI 22-50, which have hydrophobic portions and marked tendencies to produce alpha-helicity, specifically displace (apparent Ki, 5-6 microM) [3H] Ro 5-4864 from astroglial cell binding sites. Tritium 282-284 diazepam binding inhibitor Rattus norvegicus 126-129 9627847-9 1998 [3H]thymidine uptake into Peyer"s patch and spleen cells from both control and Lf-fed mice was enhanced by 75 micrograms Lf/ml in vitro, but Lf groups had a greater proliferation rate than the control group. Tritium 1-3 lactotransferrin Mus musculus 79-81 9627847-9 1998 [3H]thymidine uptake into Peyer"s patch and spleen cells from both control and Lf-fed mice was enhanced by 75 micrograms Lf/ml in vitro, but Lf groups had a greater proliferation rate than the control group. Tritium 1-3 lactotransferrin Mus musculus 121-123 9627847-9 1998 [3H]thymidine uptake into Peyer"s patch and spleen cells from both control and Lf-fed mice was enhanced by 75 micrograms Lf/ml in vitro, but Lf groups had a greater proliferation rate than the control group. Tritium 1-3 lactotransferrin Mus musculus 121-123 10728789-4 1999 These studies suggest that the K+(ATP) channel blocker, glibenclamide, modulated the adenosine A2A receptor in such a manner that [3H]CGS 21680 alone recognizes a single affinity adenosine receptor, but that the interactions between K+(ATP) channels and adenosine receptors. Tritium 131-133 adenosine A2a receptor Bos taurus 85-107 2154668-5 1990 A comparison analysis of the binding data with the secondary structure characteristics of the peptides demonstrated that TTN (DBI 17-50) and DBI 22-50, which have hydrophobic portions and marked tendencies to produce alpha-helicity, specifically displace (apparent Ki, 5-6 microM) [3H] Ro 5-4864 from astroglial cell binding sites. Tritium 282-284 diazepam binding inhibitor Rattus norvegicus 141-144 10199585-5 1999 In the presence of HG, EGF increased 3H-AA release by 24.6% after the 1st hour and by 20.4 and 19.4%, after the 2nd and 3rd hours, respectively. Tritium 37-39 epidermal growth factor Homo sapiens 23-26 10473285-5 1999 Furthermore, a temporal decrease in the double labeling of beta-galactosidase-labeled cells with [3H]thymidine was observed, indicating that only a subpopulation of the migratory subependymal-derived cells continue to actively proliferate en route to the olfactory bulb. Tritium 98-100 galactosidase, beta 1 Mus musculus 59-77 9525943-4 1998 Importantly, [3H]BZDC-triester-PtdIns(3,4,5)P3, the lipid with the same phosphorylation pattern as Ins(1,3,4,5)P4, showed specific, PtdIns(3,4,5)P3-displaceable labeling of only alpha-COP. Tritium 14-16 COPI coat complex subunit alpha Homo sapiens 178-187 9582514-5 1998 Both the IGF-I-induced protein phosphorylation and [3H]thymidine incorporation were abolished in the presence of the tyrosine kinase inhibitor, genistein. Tritium 52-54 insulin-like growth factor 1 Rattus norvegicus 9-14 9506453-6 1998 [3H]All-trans retinol (t-ROL) added to suspensions of intact epidermal cells was metabolically converted to [3H]t-RA, which bound to RAR. Tritium 1-3 retinoic acid receptor alpha Homo sapiens 133-136 2164200-17 1990 Also, [3H] WEB 2086 appears to be a suitable antagonist radioligand for labelling of PAF receptors in these inflammatory cells. Tritium 7-9 PCNA clamp associated factor Homo sapiens 85-88 9506453-6 1998 [3H]All-trans retinol (t-ROL) added to suspensions of intact epidermal cells was metabolically converted to [3H]t-RA, which bound to RAR. Tritium 109-111 retinoic acid receptor alpha Homo sapiens 133-136 9506453-10 1998 At ratios of 9c-RA to t-RA of 3:1 or lower, only [3H]t-RA was bound by RAR. Tritium 50-52 retinoic acid receptor alpha Homo sapiens 71-74 9832125-7 1998 Pretreatment of the cells with methylarachidonyl fluorophosphonate (MAFP), a phospholipase A2 (PLA2) inhibitor, almost completely prevented the release of [3H]OA by H2O2 treatment. Tritium 156-158 phospholipase A2 group IB Rattus norvegicus 77-93 9832125-7 1998 Pretreatment of the cells with methylarachidonyl fluorophosphonate (MAFP), a phospholipase A2 (PLA2) inhibitor, almost completely prevented the release of [3H]OA by H2O2 treatment. Tritium 156-158 phospholipase A2 group IB Rattus norvegicus 95-99 9506453-11 1998 Incubation of cells with [3H]9c-RA alone resulted in substantial (38%) binding of [3H]t-RA to RAR, in addition to binding of [3H]9c-RA, due to isomerization of [3H]9c-RA to [3H]t-RA. Tritium 26-28 retinoic acid receptor alpha Homo sapiens 94-97 1968295-3 1990 In nine 7-day-old rats, an acute focal hypoxic-ischemic insult produced by unilateral carotid artery ligation and subsequent exposure to 8% oxygen acutely reduced 3H-TCP binding ipsilateral to the ligation by 30% in the CA1, by 27% in the CA3, by 26% in the dentate gyrus, and by 17% in the striatum compared with values from the contralateral hemisphere. Tritium 163-165 carbonic anhydrase 1 Rattus norvegicus 220-223 9506453-11 1998 Incubation of cells with [3H]9c-RA alone resulted in substantial (38%) binding of [3H]t-RA to RAR, in addition to binding of [3H]9c-RA, due to isomerization of [3H]9c-RA to [3H]t-RA. Tritium 83-85 retinoic acid receptor alpha Homo sapiens 94-97 9506453-11 1998 Incubation of cells with [3H]9c-RA alone resulted in substantial (38%) binding of [3H]t-RA to RAR, in addition to binding of [3H]9c-RA, due to isomerization of [3H]9c-RA to [3H]t-RA. Tritium 83-85 retinoic acid receptor alpha Homo sapiens 94-97 9506453-11 1998 Incubation of cells with [3H]9c-RA alone resulted in substantial (38%) binding of [3H]t-RA to RAR, in addition to binding of [3H]9c-RA, due to isomerization of [3H]9c-RA to [3H]t-RA. Tritium 83-85 retinoic acid receptor alpha Homo sapiens 94-97 9506453-11 1998 Incubation of cells with [3H]9c-RA alone resulted in substantial (38%) binding of [3H]t-RA to RAR, in addition to binding of [3H]9c-RA, due to isomerization of [3H]9c-RA to [3H]t-RA. Tritium 83-85 retinoic acid receptor alpha Homo sapiens 94-97 9506453-13 1998 Competition studies revealed that 9c-RA was 6-fold less effective than t-RA at displacing [3H]t-RA bound to RAR in nuclear extracts. Tritium 91-93 retinoic acid receptor alpha Homo sapiens 108-111 9916894-0 1998 Labeling of dopamine D3 and D4 receptor subtypes in human peripheral blood lymphocytes with [3H]7-OH-DPAT: a combined radioligand binding assay and immunochemical study. Tritium 93-95 dopamine receptor D4 Homo sapiens 12-39 9916894-7 1998 Anti-dopamine D3 and D4 receptor antibodies reduced [3H]7-OH-DPAT binding by about 53% and 32% respectively. Tritium 53-55 dopamine receptor D4 Homo sapiens 5-32 9916894-8 1998 Combination of anti D3 and D4 receptor antibodies reduced remarkably [3H]7-OH-DPAT binding. Tritium 70-72 dopamine receptor D4 Homo sapiens 27-38 9822642-4 1998 Recombinant rat coupling factor 6, which was synthesized using a cleavable fusion protein strategy, attenuated base-line and bradykinin (10(-6) M)-induced prostacyclin synthesis and [3H]arachidonic acid (AA) release in human umbilical vein endothelial cells in a dose-dependent manner (10(-9)-10(-7) M). Tritium 183-185 ATP synthase peripheral stalk subunit F6 Rattus norvegicus 16-33 2298497-7 1990 Furthermore, anti-EGF (KEM-10) and anti-TGF-alpha (WA-3) monoclonal antibodies (MAbs) inhibited spontaneous uptake of tritiated thymidine (3H-TdR) by TE-1 cells which expressed EGF, TGF-alpha and EGFR mRNA and protein. Tritium 139-141 epidermal growth factor Homo sapiens 18-21 9792625-4 1998 In the absence of the omega-NG, NG-[3H]dimethylarginine product of the RMT1 methyltransferase, we were able to detect a previously obscured 3H-methylated species that migrated in the region of methylated arginine derivatives. Tritium 36-38 protein-arginine omega-N methyltransferase HMT1 Saccharomyces cerevisiae S288C 71-75 9448281-6 1998 In parallel experiments performed on cells transfected with lacZ (control) or ACVI, [3H]forskolin binding, used to assess AC protein expression, was amplified 6-fold, while betaAR-stimulated cAMP production from these cells was increased 7-fold. Tritium 85-87 adenylate cyclase 6 Rattus norvegicus 78-82 9792625-4 1998 In the absence of the omega-NG, NG-[3H]dimethylarginine product of the RMT1 methyltransferase, we were able to detect a previously obscured 3H-methylated species that migrated in the region of methylated arginine derivatives. Tritium 140-142 protein-arginine omega-N methyltransferase HMT1 Saccharomyces cerevisiae S288C 71-75 2298497-7 1990 Furthermore, anti-EGF (KEM-10) and anti-TGF-alpha (WA-3) monoclonal antibodies (MAbs) inhibited spontaneous uptake of tritiated thymidine (3H-TdR) by TE-1 cells which expressed EGF, TGF-alpha and EGFR mRNA and protein. Tritium 139-141 epidermal growth factor Homo sapiens 177-180 9808692-11 1998 The pharmacological and regional comparisons suggest that the nAChR that stimulates [3H]-GABA release is the one that binds [3H]-nicotine with high affinity (alpha4beta2). Tritium 85-87 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 62-67 2252308-5 1990 5-HT1 receptor subtypes are labeled with [3H]5-HT and present a large heterogeneity: no less than 4 subtypes have been characterized: 5-HT1B and 5-HT1D are labeled respectively with [125I]cyanopindolol, and [3H]5-HT under appropriate conditions. Tritium 42-44 5-hydroxytryptamine receptor 1D Rattus norvegicus 145-151 9808692-11 1998 The pharmacological and regional comparisons suggest that the nAChR that stimulates [3H]-GABA release is the one that binds [3H]-nicotine with high affinity (alpha4beta2). Tritium 125-127 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 62-67 9845278-5 1998 It is concluded that membranes isolated from HEK 293 cells stably transfected with alpha1beta2gamma2s subunits exhibit specific high-affinity [3H]TBOB binding. Tritium 143-145 adrenoceptor alpha 1D Homo sapiens 83-100 9806343-17 1998 The properties of the [3H]-P1075 site strongly suggest that SUR2B represents the drug receptor of the openers in vascular smooth muscle. Tritium 23-25 ATP-binding cassette, sub-family C (CFTR/MRP), member 9 Mus musculus 60-65 9737879-3 1998 Purified triadin not only inhibited [3H]ryanodine binding to the solubilized HSR but also reduced openings of purified RyR incorporated into the planar lipid bilayers. Tritium 37-39 triadin Oryctolagus cuniculus 9-16 9737879-13 1998 Ca2+ dependency of [3H]ryanodine binding to the solubilized HSR was reduced by triadin, whereas that was enhanced by calsequestrin. Tritium 20-22 triadin Oryctolagus cuniculus 79-86 9737879-14 1998 Interestingly, [3H]ryanodine binding to the solubilized HSR potentiated by calsequestrin was reduced by triadin. Tritium 16-18 triadin Oryctolagus cuniculus 104-111 9458369-2 1998 By performing receptor binding assays on human AGS gastric cancer cells with the selective CCK-B/gastrin receptor antagonist [3H]L-365,260, specific and saturable binding were determined. Tritium 126-128 gastrin Homo sapiens 97-104 9457843-12 1998 Some of the other pheromones and inhibitors inhibited [3H]cPD1 binding to the traC transformant like cPD1 and iPD1 did. Tritium 55-57 cerebellar ataxia, infantile nonprogressive, autosomal recessive Homo sapiens 58-62 9426700-9 1998 Furthermore, the selective inhibition of FR-alpha and RFC functionality allowed us to distinguish their differential role on the overall N5-CH3-[3H]H4PteGlu intracellular delivery. Tritium 145-147 rabaptin, RAB GTPase binding effector protein 2 Homo sapiens 41-49 9973639-6 1998 Partially glycosylated Rh50 (32 kDa) was again isolated from the lysates of K562 cells metabolically labeled with [35S]-methionine or [3H]-mannose using anti-Rh50 antisera. Tritium 135-137 Rh associated glycoprotein Homo sapiens 23-27 9473697-4 1998 This was associated with significantly higher [3H]8-OH-DPAT binding in the inferior part of CA1. Tritium 47-49 carbonic anhydrase 1 Rattus norvegicus 92-95 2175579-6 1990 3H-thymidine uptake increased by about 110-376% of control after IL-1 treatment. Tritium 0-2 interleukin 1 alpha Homo sapiens 65-69 9730889-6 1998 The NP1 product displaced approximately 90% of the 3H-Arg-labeled endogenous peptide fraction in B27(+)NP1(+) spleen cells. Tritium 51-53 neuronal pentraxin 1 Rattus norvegicus 4-7 2262070-6 1990 This was verified by measuring [3H]DFMO binding; serum-stimulated induction of activity was accompanied by a corresponding 20-fold increase in the specific binding of DFMO to ODC. Tritium 31-34 ornithine decarboxylase 1 Rattus norvegicus 175-178 9722550-4 1998 The purified phosphodiester alpha-GlcNAcase has a specific activity of 498 micromol of [3H]GlcNAc-alpha-phosphomannose-alpha-methyl cleaved per h per mg of protein using 0.5 mM [3H]GlcNAc-alpha-phosphomannose-alpha-methyl as substrate. Tritium 88-90 N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase Bos taurus 13-43 9762008-8 1998 IGF-I and IGF-II (0.3-30 nM) dose-dependently stimulated [3H]thymidine incorporation, with a maximal response of 110% above basal. Tritium 58-60 insulin like growth factor 2 Homo sapiens 10-16 2312012-2 1990 alpha-[3H]T-2 toxin was prepared by oxidation of T-2 toxin and reduction with [3H]NaBH4 followed by normal phase and reverse phase high-performance liquid chromatography. Tritium 7-9 brachyury 2 Mus musculus 10-13 9785467-5 1998 The aim of the study was to determine the effects of IAPP on IGF-I-stimulated rates of glucose transport and metabolism (measured by 3-O-methyl[3H]glucose and [U-14C]glucose, respectively) in rat soleus muscle, and compare them with those simulated by insulin. Tritium 144-146 insulin-like growth factor 1 Rattus norvegicus 61-66 2312012-2 1990 alpha-[3H]T-2 toxin was prepared by oxidation of T-2 toxin and reduction with [3H]NaBH4 followed by normal phase and reverse phase high-performance liquid chromatography. Tritium 7-9 brachyury 2 Mus musculus 49-52 9754915-4 1998 Following transient global ischemia, [3H]L-N(G)-nitroarginine binding showed a significant increase in the striatum (17-18%) and hippocampal CA1 sector (24%) at 48 and 24 h after recirculation, respectively. Tritium 38-40 carbonic anhydrase 1 Rattus norvegicus 141-144 2159762-3 1990 CSF-1 increased [3H]thymidine incorporation in serum-starved cells and potentiated the mitogenic effects of FGF and thrombin. Tritium 17-19 colony stimulating factor 1 Homo sapiens 0-5 9733635-9 1998 For IGF-1, maximal [3H]thymidine incorporation was only 70% of the maximal effect observed for the other growth factors tested. Tritium 20-22 insulin-like growth factor I Oryctolagus cuniculus 4-9 2358773-3 1990 The amount of 3H-amino acids incorporated in rdgA rhabdomeres at 3 hr after the injection was about 50%, and that of 3H-sugar residues was about 20% of normal. Tritium 14-16 retinal degeneration A Drosophila melanogaster 45-49 9687571-4 1998 Dose-response experiments indicated that the high level of expression of hGSTP1-1 in the T47Dpi cell line (4411 +/- 183 milliunits/mg of cytosolic protein, using 1-Cl-2,4-dinitrobenzene as substrate), resulted in 70-90% reduction in the covalent 3H-adduct formation in DNA or RNA isolated from the GSTP1-transfected T47Dpi cell line. Tritium 246-248 glutathione S-transferase pi 1 Homo sapiens 73-81 2108945-7 1990 Moreover, anti-EGF and anti-TGF-alpha monoclonal antibodies inhibited the spontaneous 3H-TdR uptake by gastric carcinoma cells. Tritium 86-88 epidermal growth factor Homo sapiens 15-18 9687571-4 1998 Dose-response experiments indicated that the high level of expression of hGSTP1-1 in the T47Dpi cell line (4411 +/- 183 milliunits/mg of cytosolic protein, using 1-Cl-2,4-dinitrobenzene as substrate), resulted in 70-90% reduction in the covalent 3H-adduct formation in DNA or RNA isolated from the GSTP1-transfected T47Dpi cell line. Tritium 246-248 glutathione S-transferase pi 1 Homo sapiens 74-79 2374438-3 1990 In B-lineage ALL, a significant increase in 3H-TdR incorporation was observed in 5/12 cases (42%) in the presence of rIL-1 alpha, in 10/12 cases (83%) with rIL-2, in 9/12 cases (75%) with rIL-3, in 3/6 cases (50%) with rIL-4, in 4/6 cases (67%) with rIL-5, and in 4/12 cases (33%) with rIL-6. Tritium 44-46 interleukin 2 Rattus norvegicus 156-161 9660787-3 1998 To establish how it is glycosylated, SKP1 labeled with [3H]Fuc was purified to homogeneity and digested with endo-Lys-C. Tritium 56-58 S-phase kinase associated protein 1 Homo sapiens 37-41 1701523-10 1990 In the CA1 subfield, subnormal activity of [3H]phorbol 12,13-dibutyrate and [3H]forskolin binding were observed in spite of the depleted [3H]inositol 1,4,5-triphosphate binding. Tritium 44-46 carbonic anhydrase 1 Rattus norvegicus 7-10 9879066-0 1998 [3H]-BIBP3226 and [3H]-NPY binding to intact SK-N-MC cells and CHO cells expressing the human Y1 receptor. Tritium 19-21 neuropeptide Y Homo sapiens 23-26 9879066-6 1998 Whilst the NPY Y1 agonists NPY, PYY and [Leu31-Pro34]-NPY completely and potently displaced [3H]-NPY binding, they could only displace 70 to 80% of the [3H]-BIBP3226 binding sites in CHO-Y1 and SK-N-MC cells. Tritium 93-95 neuropeptide Y Homo sapiens 11-14 9879066-6 1998 Whilst the NPY Y1 agonists NPY, PYY and [Leu31-Pro34]-NPY completely and potently displaced [3H]-NPY binding, they could only displace 70 to 80% of the [3H]-BIBP3226 binding sites in CHO-Y1 and SK-N-MC cells. Tritium 93-95 neuropeptide Y Homo sapiens 27-30 9879066-6 1998 Whilst the NPY Y1 agonists NPY, PYY and [Leu31-Pro34]-NPY completely and potently displaced [3H]-NPY binding, they could only displace 70 to 80% of the [3H]-BIBP3226 binding sites in CHO-Y1 and SK-N-MC cells. Tritium 93-95 neuropeptide Y Homo sapiens 27-30 9879066-6 1998 Whilst the NPY Y1 agonists NPY, PYY and [Leu31-Pro34]-NPY completely and potently displaced [3H]-NPY binding, they could only displace 70 to 80% of the [3H]-BIBP3226 binding sites in CHO-Y1 and SK-N-MC cells. Tritium 93-95 neuropeptide Y Homo sapiens 27-30 9879066-6 1998 Whilst the NPY Y1 agonists NPY, PYY and [Leu31-Pro34]-NPY completely and potently displaced [3H]-NPY binding, they could only displace 70 to 80% of the [3H]-BIBP3226 binding sites in CHO-Y1 and SK-N-MC cells. Tritium 153-155 neuropeptide Y Homo sapiens 11-14 9658403-12 1998 The nuclear 9S-[3H]Org2058-PR resulting from cells exposed to Rap, contained, in addition to the heat shock proteins of 90 kDa and 70 kDa (hsp90 and hsp70), the FK506-binding immunophilin FKBP52 but not FKBP51, although the latter was part of unliganded PR heterocomplex associated with hsp90. Tritium 16-18 heat shock protein 90 alpha family class A member 1 Homo sapiens 139-144 9658403-12 1998 The nuclear 9S-[3H]Org2058-PR resulting from cells exposed to Rap, contained, in addition to the heat shock proteins of 90 kDa and 70 kDa (hsp90 and hsp70), the FK506-binding immunophilin FKBP52 but not FKBP51, although the latter was part of unliganded PR heterocomplex associated with hsp90. Tritium 16-18 heat shock protein 90 alpha family class A member 1 Homo sapiens 287-292 9690735-5 1998 The results showed that 1500 nM [3H]ouabain binding was sensitive to serotonin 10(-3) M and significantly increased in the following brain regions: frontal cortex, areas CA1, CA2, and CA3 of the hippocampus, presubiculum, zona incerta, caudate putamen and the amygdaloid area, confirming and extending previous results. Tritium 33-35 carbonic anhydrase 1 Rattus norvegicus 170-173 9633917-7 1998 The effect of myotrophin on the stimulation of PKC activity and [3H]leucine incorporation was abolished by pretreatment with either staurosporine or H-7, two selective, pharmacological PKC inhibitors. Tritium 65-67 myotrophin Rattus norvegicus 14-24 2166246-4 1990 [3H]Glycine interacted with a single population of sites having a KD of approximately 200 nM and a maximum density of 6.2 pmol/mg protein (stratum radiatum, CA1). Tritium 1-3 carbonic anhydrase 1 Rattus norvegicus 157-160 9633917-10 1998 Genistein attenuated the [3H]leucine incorporation induced by myotrophin. Tritium 26-28 myotrophin Rattus norvegicus 62-72 2199843-7 1990 Finally, glial-enriched cultures (greater than 99% glial cells) obtained from the fetal hypothalamus showed increased [3H]thymidine incorporation in response to insulin-like growth factor-I. Tritium 119-121 insulin-like growth factor 1 Rattus norvegicus 161-189 9696425-4 1998 administration to CD1 mice, [3H]naltriben uptake and retention were high in striatum, cortical regions and olfactory tubercles, and low in superior colliculi and cerebellum. Tritium 29-31 CD1 antigen complex Mus musculus 18-21 2153287-6 1990 In both species, NGFR immunoreactivity is primarily associated with the early generated and transient subplate neuron population of the developing neocortex, as indicated by the following evidence: the immunoreactive cells (i) are located directly beneath the developing cortical plate, (ii) frequently have the inverted pyramid shape characteristic of subplate neurons, and (iii) can be labeled by an injection of [3H]thymidine on embryonic day (E) 28, a time when only subplate neurons are being generated. Tritium 416-418 nerve growth factor receptor Homo sapiens 17-21 9834621-5 1998 With 50 micrograms of G-CSF, the [3H] thymidine uptake was 11,100 +/- 2,200 DPM and the control uptake was 7,970 +/- 1,820 DPM. Tritium 34-36 colony stimulating factor 3 Homo sapiens 22-27 9630347-4 1998 SDZ NKT 343 inhibited [3H]-substance P binding to the human NK1 receptor in transfected Cos-7 cell membranes (IC50 = 0.62+/-0.11 nM). Tritium 23-25 tachykinin receptor 1 Homo sapiens 60-72 9573065-3 1998 Incubation of isolated neutrophils with [3H]AA resulted in incorporation of radioactivity in the PLA2 substrates phosphatidylcholine, phosphatidylinositol, and phosphatidylethanolamine. Tritium 41-43 LOC104974671 Bos taurus 97-101 1689493-0 1990 Substance P modulation of [3H]DAGO binding in the brain of Swiss-Webster and CXBK (mu opioid receptor deficient) mice. Tritium 27-29 tachykinin 1 Mus musculus 0-11 9556603-8 1998 [3H]CGP 61594 is a promising tool to identify the NR2 subunit domains that contribute to differential glycine antagonist sites of NMDA receptor subtypes. Tritium 1-3 glutamate ionotropic receptor NMDA type subunit 1 L homeolog Xenopus laevis 130-143 2301012-2 1990 However, prior to target cell death very low concentrations of rIL-2 and IL-2 toxin synergistically stimulate [3H] thymidine incorporation despite inhibition of [14C] leucine uptake. Tritium 111-113 interleukin 2 Rattus norvegicus 63-68 9679569-7 1998 Similarly, V79MZr1A1 cells that express CYP1A1 and either transfected human or murine GSTP1-1 (< 5000 mIU/mg, CDNB) exhibited > 70% decrease in covalent labeling of total nucleic acids by [3H]BPDE. Tritium 195-197 cytochrome P450 family 1 subfamily A member 1 Homo sapiens 40-46 10227851-4 1999 Several oxy- and thio-barbiturates inhibited [3H]-2-deoxyglucose uptake by GLUT-1 expressing murine fibroblasts with IC50s of 0.2-2.9 mm. Tritium 46-48 solute carrier family 2 (facilitated glucose transporter), member 1 Mus musculus 75-81 9679569-7 1998 Similarly, V79MZr1A1 cells that express CYP1A1 and either transfected human or murine GSTP1-1 (< 5000 mIU/mg, CDNB) exhibited > 70% decrease in covalent labeling of total nucleic acids by [3H]BPDE. Tritium 195-197 glutathione S-transferase, pi 1 Mus musculus 86-93 9730266-10 1998 [3H]-Spiperone binding was more sensitive to displacement by the neuroleptic clozapine in sections of atria than of ventricles, suggesting the expression of a dopamine D4 receptor in atrial tissue. Tritium 1-3 dopamine receptor D4 Homo sapiens 159-179 9730266-11 1998 Moreover, preincubation of some sections with a dopamine D4 receptor antibody and subsequent exposure to [3H]-spiperone caused a remarkable decrease of radioligand binding to sections of atria, but only a slight reduction of binding to sections of ventricles. Tritium 106-110 dopamine receptor D4 Homo sapiens 48-68 34627806-0 2021 In vitro characterization of (3H)VAT in cells, animal and human brain tissues for vesicular acetylcholine transporter. Tritium 30-32 solute carrier family 18 member A3 Homo sapiens 82-117 9614352-9 1998 In the absence of TSH, incubation with VEGF caused a significant reduction in [3H]thymidine incorporation, but did not significantly alter [125I] uptake. Tritium 79-81 vascular endothelial growth factor A Rattus norvegicus 39-43 9614352-10 1998 Incubation with TSH (1 mU/ml) caused a fourfold increase in [3H]thymidine incorporation that was diminished by co-incubation with 10 ng/ml or greater VEGF. Tritium 61-63 vascular endothelial growth factor A Rattus norvegicus 150-154 9614352-12 1998 The antagonistic effects of VEGF on TSH-stimulated [3H]thymidine incorporation or [125I] uptake were significantly reduced in the presence of an anti-VEGF antiserum. Tritium 52-54 vascular endothelial growth factor A Rattus norvegicus 28-32 34627806-4 2021 Using human VAChT-rich cell membrane extracts, a saturated binding curve was obtained for (3H)VAT with Kd = 6.5 nM and Bmax = 22.89 pmol/mg protein. Tritium 91-93 solute carrier family 18 member A3 Homo sapiens 12-17 34627806-5 2021 In the (3H)VAT competition-binding assay with a panel of CNS ligands, binding inhibition of (3H)VAT was observed using VAChT ligands, the Ki values ranged from 5.41 to 33.3 nM. Tritium 8-10 solute carrier family 18 member A3 Homo sapiens 119-124 9606055-2 1998 In this assay we employed [3H]urocortin as the radioligand and, as a means to separate bound and free ligand, adsorption to activated charcoal. Tritium 27-29 urocortin Homo sapiens 30-39 34627806-5 2021 In the (3H)VAT competition-binding assay with a panel of CNS ligands, binding inhibition of (3H)VAT was observed using VAChT ligands, the Ki values ranged from 5.41 to 33.3 nM. Tritium 93-95 solute carrier family 18 member A3 Homo sapiens 119-124 9606055-7 1998 A range of CRF-related peptides potently competed for specific [3H]urocortin binding. Tritium 64-66 urocortin Homo sapiens 67-76 9753183-7 1998 More importantly, GT1 neurons took up and stored 3H-GABA. Tritium 49-51 beta-1,4-galactosyltransferase 1 Homo sapiens 18-21 9587785-10 1998 The pretreatment of rats with Vit.E reduced the Fe-NTA-mediated induction in ODC activity and enhancement in [3H]thymidine incorporation in DNA. Tritium 110-112 vitrin Rattus norvegicus 30-33 34627806-9 2021 In the competitive (3H)VAT ARG with human striatal sections, strong ARG signals in caudate and putamen were blocked significantly by either VAChT ligand TZ659 or (-)-vesamicol, but not by the sigma1 receptor ligand Yun-122. Tritium 20-22 solute carrier family 18 member A3 Homo sapiens 140-145 35631399-4 2022 The in vitro screening results show that two purine derivatives, 3d and 3h, elicit high affinities to the H3R (Ki values of 2.91 and 5.51 nM, respectively). Tritium 72-74 histamine receptor H3 Homo sapiens 106-109 9525346-9 1998 Assay of VDR levels by L-1-tosylamido-2-phenylethyl chloromethyl ketone-3H-1,25(OH)2D3 exchange assay ruled out differences in endogenous 1,25(OH)2D3 occupancy as the basis for the observed differences in VDR regulation. Tritium 72-74 vitamin D receptor Rattus norvegicus 9-12 9475512-4 1998 produced significant increases in [3H]CCPA binding in CA1 subarea at seizure (15%) and postseizure (21%) and in CA2 at seizure (15%) but a tendency to decrease in dentate gyrus. Tritium 35-37 carbonic anhydrase 1 Rattus norvegicus 54-57 9475512-7 1998 enhanced [3H]CCPA binding in CA1 and CA2 areas (17-18%) but not in CA3 area of the hippocampus. Tritium 10-12 carbonic anhydrase 1 Rattus norvegicus 29-32 35547552-7 2022 Although there was no significant correlation between these three assays in the healthy controls, the SI value for <10% (3H)-thymidine proliferation in those with T cell deficiency was compatible with CFSE- and Ki-67-stained lymphocyte percentages, and validated in patients with IL2RG, RAG1, and ZAP70 mutations. Tritium 121-123 recombination activating 1 Homo sapiens 287-291 9475512-8 1998 When CPA was administered before MP, which delayed seizure onset, an increase in [3H]CCPA binding in CA1 hippocampus subarea (19%) and cerebellum (28%) was also observed. Tritium 82-84 carbonic anhydrase 1 Rattus norvegicus 101-104 35362514-8 2022 IL-1beta and IL-6 were up-regulated after 1h, but IL-1beta decreased right after 3h, while IL-6 sustained up-regulation throughout all time points. Tritium 81-83 interleukin 1 alpha Homo sapiens 0-8 9492296-5 1998 Bio-Gel P-6 column chromatography of a mixture of the tritium-labeled glycopeptide/oligosaccharides obtained by pronase-digestion followed by mild alkaline borohydride treatment showed that distribution of the sialylated core 2 oligosaccharides is different among CD45 isoforms. Tritium 54-61 exosome component 9 Homo sapiens 8-11 35224872-5 2022 Molecular analyses indicate that whereas SGK1-mediated phosphorylation of the ubiquitin-protein ligase NEDD4-2 begins to increase by 3h, SGK1-dependent proteolytic activation of ENaC only becomes detectable after 6 h of HK intake. Tritium 133-135 serum/glucocorticoid regulated kinase 1 Mus musculus 41-45 9543241-2 1998 MEN 11149 potently inhibits the binding of [3H]substance P to tachykinin NK1 sites in IM9 cells (pKi = 8.5 +/- 0.1). Tritium 44-46 tachykinin receptor 1 Homo sapiens 73-76 35224872-5 2022 Molecular analyses indicate that whereas SGK1-mediated phosphorylation of the ubiquitin-protein ligase NEDD4-2 begins to increase by 3h, SGK1-dependent proteolytic activation of ENaC only becomes detectable after 6 h of HK intake. Tritium 133-135 neural precursor cell expressed, developmentally down-regulated gene 4-like Mus musculus 103-110 9662143-3 1998 Analysis of the saturation kinetics of 3H-AMPA binding revealed that the biphasic effect of PLA2 was due to modifications in receptor affinity and not to changes in the maximum number of binding sites for AMPA receptors. Tritium 39-41 phospholipase A2 group IB Rattus norvegicus 92-96 2574996-4 1989 In order to investigate the mechanism by which the cytolysin causes cell lysis, we have prepared a highly active [3H]cytolysin derivative by reductive methylation with sodium cyanoborohydride and [3H]formaldehyde. Tritium 114-116 perforin 1 Homo sapiens 51-60 9575420-5 1998 The calculated IC50 values were 70 nM and 100 nM of hCRF, as measured by digital videomicroscopic quantitation of tumor cell population growth rate and by [3H]-thymidine incorporation assay, respectively. Tritium 156-158 corticotropin releasing hormone Homo sapiens 52-56 9458889-9 1998 Inhibition of the FAAH with diazomethylarachidonyl ketone blocked both the metabolism of [3H]AEA and the relaxations to AEA. Tritium 90-92 fatty-acid amide hydrolase 1 Bos taurus 18-22 9445257-7 1998 The inhibition of the stimulatory effect of BP II on cholesterol production by genistein in hyperapoB cells may be mediated through 3-hydroxy 3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis, since the rate of incorporation of [14C]acetate, but not [3H]mevalonate, into unesterified cholesterol was decreased by genistein in the hyperapoB cells. Tritium 293-295 3-hydroxy-3-methylglutaryl-CoA reductase Homo sapiens 132-179 2574996-4 1989 In order to investigate the mechanism by which the cytolysin causes cell lysis, we have prepared a highly active [3H]cytolysin derivative by reductive methylation with sodium cyanoborohydride and [3H]formaldehyde. Tritium 114-116 perforin 1 Homo sapiens 117-126 9496737-8 1998 With the use of several substrates labeled with tritium, GMP was observed to degrade type IV collagen, fibronectin and casein as well as gelatin, but not type I collagen. Tritium 48-55 fibronectin 1 Rattus norvegicus 103-114 2574996-4 1989 In order to investigate the mechanism by which the cytolysin causes cell lysis, we have prepared a highly active [3H]cytolysin derivative by reductive methylation with sodium cyanoborohydride and [3H]formaldehyde. Tritium 197-199 perforin 1 Homo sapiens 51-60 9718083-2 1998 Scatchard analysis of [3H]-ryanodine binding to the membrane from CHO cells expressing RyR3 showed two distinct binding sites. Tritium 23-25 LOW QUALITY PROTEIN: ryanodine receptor 3 Cricetulus griseus 87-91 2574996-4 1989 In order to investigate the mechanism by which the cytolysin causes cell lysis, we have prepared a highly active [3H]cytolysin derivative by reductive methylation with sodium cyanoborohydride and [3H]formaldehyde. Tritium 197-199 perforin 1 Homo sapiens 117-126 9718083-4 1998 [3H]-ryanodine binding to the membrane from CHO cells expressing RyR3 was dependent on pCa. Tritium 1-3 LOW QUALITY PROTEIN: ryanodine receptor 3 Cricetulus griseus 65-69 9718083-6 1998 The enhancement of [3H]-ryanodine binding to the membrane from CHO cells expressing RyR3 was observed by bromoeudistomin D (BED), a caffeine-like powerful Ca2+ releaser, at pCa 5.5. Tritium 20-22 LOW QUALITY PROTEIN: ryanodine receptor 3 Cricetulus griseus 84-88 9434783-2 1997 Leptin also increased DNA synthesis and cell viability in MIN6 cells based on the results of [3H]-thymidine incorporation and colorimetric MTT assay, respectively. Tritium 94-96 leptin Mus musculus 0-6 2606857-1 1989 The concentration of Na-K pumps was measured as the total capacity for [3H]ouabain binding in needle biopsies of the vastus lateralis muscle. Tritium 72-74 TANK binding kinase 1 Homo sapiens 21-25 9434130-8 1997 PAF (1-100 nM) stimulated [3H]thymidine incorporation in K562 cells grown in low serum concentration, a non-metabolizable PAF agonist being more potent than PAF to stimulate thymidine incorporation. Tritium 27-29 PCNA clamp associated factor Homo sapiens 0-3 9434132-2 1997 Expression of this receptor was demonstrated with specific binding of the mu-opioid selective ligand [3H][D-Ala2,N-MePhe4,Gly5-ol]-enkephalin ([3H]DAMGO). Tritium 102-104 proenkephalin Rattus norvegicus 131-141 9347319-2 1997 The binding characteristics of tritium labeled 8-hydroxy-dipropyl-aminotetralin, or [3H]8-OH-DPAT, to the serotonin1A (5-HT1A) receptor in the stably transfected HeLa cell clone HA6 and in human cortical tissue were examined and compared. Tritium 31-38 5-hydroxytryptamine receptor 1A Homo sapiens 106-135 9347319-2 1997 The binding characteristics of tritium labeled 8-hydroxy-dipropyl-aminotetralin, or [3H]8-OH-DPAT, to the serotonin1A (5-HT1A) receptor in the stably transfected HeLa cell clone HA6 and in human cortical tissue were examined and compared. Tritium 84-87 5-hydroxytryptamine receptor 1A Homo sapiens 106-135 9700759-3 1998 Quantitative binding studies resolved two high-affinity [3H][D-Ala2,D-Leu5]enkephalin binding sites in rat brain membranes depleted of mu binding sites by pretreatment with the irreversible agent BIT. Tritium 57-60 proenkephalin Rattus norvegicus 75-85 9700759-8 1998 Under these assay conditions, [3H][D-Ala2,D-Leu5]enkephalin binding to the cells expressing the cloned mu receptor is very low and pretreatment of cell membranes with BIT almost completely inhibits [3H]DAMGO and [3H][D-Ala2,D-Leu5]enkephalin binding. Tritium 31-33 proenkephalin Rattus norvegicus 49-59 9700759-8 1998 Under these assay conditions, [3H][D-Ala2,D-Leu5]enkephalin binding to the cells expressing the cloned mu receptor is very low and pretreatment of cell membranes with BIT almost completely inhibits [3H]DAMGO and [3H][D-Ala2,D-Leu5]enkephalin binding. Tritium 199-201 proenkephalin Rattus norvegicus 49-59 9700759-8 1998 Under these assay conditions, [3H][D-Ala2,D-Leu5]enkephalin binding to the cells expressing the cloned mu receptor is very low and pretreatment of cell membranes with BIT almost completely inhibits [3H]DAMGO and [3H][D-Ala2,D-Leu5]enkephalin binding. Tritium 199-201 proenkephalin Rattus norvegicus 49-59 9700759-9 1998 Intracerebroventricular administration of antisense DNA to the cloned delta receptor selectively decreased [3H][D-Ala2,D-Leu5]enkephalin binding to the delta ncx-1 site. Tritium 108-110 proenkephalin Rattus norvegicus 126-136 9330371-13 1997 However, neurotrophin-3 (10 micrograms/day) delivery into the striatum significantly increased [3H]GABA uptake, but only modestly affected [3H]choline uptake. Tritium 96-98 neurotrophin 3 Rattus norvegicus 9-23 9330371-13 1997 However, neurotrophin-3 (10 micrograms/day) delivery into the striatum significantly increased [3H]GABA uptake, but only modestly affected [3H]choline uptake. Tritium 140-142 neurotrophin 3 Rattus norvegicus 9-23 9390636-5 1997 Administration of the PDE3 inhibitors cilostazol or cilostamide together with the PDE4 inhibitor rolipram blocked mitogenic synthesis of DNA, as determined by (3H)-thymidine incorporation into renal cortical DNA, in FA-treated rats. Tritium 160-162 phosphodiesterase 4D, cAMP-specific-like 1 Rattus norvegicus 22-26 2480603-1 1989 Cell proliferation in the bone marrow and blood of two patients with metastatic breast cancer who were treated with granulocyte colony-stimulating factor was studied by using [3H]thymidine labeling and autoradiography. Tritium 176-178 colony stimulating factor 3 Homo sapiens 116-153 9389377-7 1997 Whole brain membranes were prepared from separate groups of rats for determination of binding parameters of [3H][D-Ala2, N-Me-Phe4,Gly5-ol]-enkephalin (DAMGO). Tritium 109-111 proenkephalin Rattus norvegicus 140-150 9398055-4 1997 Estrogen receptor (ER) levels measured by [3H]-labeled estrogen binding were markedly reduced (by 96%, 96% and 92%) in ZR-75-1 and MCF-7 tumors, and in the mouse uterus, respectively. Tritium 43-45 estrogen receptor 1 (alpha) Mus musculus 0-17 9398055-4 1997 Estrogen receptor (ER) levels measured by [3H]-labeled estrogen binding were markedly reduced (by 96%, 96% and 92%) in ZR-75-1 and MCF-7 tumors, and in the mouse uterus, respectively. Tritium 43-45 estrogen receptor 1 (alpha) Mus musculus 19-21 9332449-1 1997 The exposure of confluent peritubular (PT) cells from immature rat testis to insulin-like growth factor-1 (IGF-1) induced a time and dose-dependent increase of [35S]-sulfate and [3H]-D-glucosamine incorporations in newly synthesized proteoglycans (PG). Tritium 179-181 insulin-like growth factor 1 Rattus norvegicus 77-105 2575494-5 1989 Tritium-labeled glucuronides obtained from the bile of rats administered [3H]T-2 toxin intravascularly were extracted and purified using C-18 and silica column chromatography. Tritium 0-7 brachyury 2 Rattus norvegicus 77-80 9332449-1 1997 The exposure of confluent peritubular (PT) cells from immature rat testis to insulin-like growth factor-1 (IGF-1) induced a time and dose-dependent increase of [35S]-sulfate and [3H]-D-glucosamine incorporations in newly synthesized proteoglycans (PG). Tritium 179-181 insulin-like growth factor 1 Rattus norvegicus 107-112 9394125-14 1997 (Hepatology 5:224-232, 1985), small, but significant, increases in the binding of [3H]MK-801 were seen in the CA1 and dentate gyrus regions of the hippocampus. Tritium 83-85 carbonic anhydrase 1 Rattus norvegicus 110-113 2575494-5 1989 Tritium-labeled glucuronides obtained from the bile of rats administered [3H]T-2 toxin intravascularly were extracted and purified using C-18 and silica column chromatography. Tritium 74-76 brachyury 2 Rattus norvegicus 77-80 9334194-8 1997 That sterol 27-hydroxylase is responsible not only for 27-hydroxylation of cholesterol but also for the further oxidation of this steroid into 3beta-hydroxy-5-cholestenoic acid was shown with use of tritium-labeled 27-hydroxycholesterol and an inhibitor of sterol 27-hydroxylase. Tritium 199-206 cytochrome P450 family 27 subfamily A member 1 Homo sapiens 5-26 9334194-8 1997 That sterol 27-hydroxylase is responsible not only for 27-hydroxylation of cholesterol but also for the further oxidation of this steroid into 3beta-hydroxy-5-cholestenoic acid was shown with use of tritium-labeled 27-hydroxycholesterol and an inhibitor of sterol 27-hydroxylase. Tritium 199-206 cytochrome P450 family 27 subfamily A member 1 Homo sapiens 257-278 9283008-5 1997 Both IGF-I and LR3-IGF-I stimulated [3H]thymidine incorporation in cumulus cells to a much greater extent than in mural granulosa cells. Tritium 37-39 insulin like growth factor 1 Bos taurus 5-10 9283008-5 1997 Both IGF-I and LR3-IGF-I stimulated [3H]thymidine incorporation in cumulus cells to a much greater extent than in mural granulosa cells. Tritium 37-39 insulin like growth factor 1 Bos taurus 19-24 2478607-3 1989 PAF, in concentrations of 5 to 100 ng/ml, induced a specific, dose-dependent release of [3H]RGC from human airways in vitro (range of 15% to 120% increase above control, p less than 0.001; n = 8). Tritium 89-91 PCNA clamp associated factor Homo sapiens 0-3 9629363-3 1997 The results show that EGF (10 ng/ml) significantly stimulates the incorporation of [3H]-thymidine in the SK-N-SH cells only in the presence of fetal bovine serum (FBS) (control = 58,285 +/- 9327 cpm; EGF = 75,523 +/- 4457, p < 0.05). Tritium 84-86 epidermal growth factor Homo sapiens 22-25 9322928-3 1997 Exogenous IGF-I synergistically and dose dependently enhanced OP-1 action in stimulating [3H]thymidine incorporation, alkaline phosphatase activity, PTH-dependent cAMP level, and bone nodule formation. Tritium 90-92 insulin-like growth factor 1 Rattus norvegicus 10-15 9233651-7 1997 Specific PDE3 (cilostamide) and PDE4 (rolipram) inhibitors suppressed [3H]thymidine incorporation in TCC. Tritium 71-73 sideroflexin 1 Homo sapiens 101-104 2553458-8 1989 FN and collagen IV also stimulated modest increases in [3H]thymidine uptake and cell number by quiescent cells. Tritium 56-58 fibronectin 1 Rattus norvegicus 0-2 9268055-6 1997 Phosphorylation and dephosphorylation of BBM with protein kinase A (PKA) and alkaline phosphatase resulted in increases and decreases in Bmax of [3H]phlorizin, respectively. Tritium 146-148 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 50-66 9268055-6 1997 Phosphorylation and dephosphorylation of BBM with protein kinase A (PKA) and alkaline phosphatase resulted in increases and decreases in Bmax of [3H]phlorizin, respectively. Tritium 146-148 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 68-71 9244236-4 1997 Retroviral vectors bearing an antisense cyclin G1 construct inhibited the proliferation of transduced aortic SMCs in 2- to 6-day cultures, concomitant with down-regulation of cyclin G1 protein expression and decreased [3H]thymidine incorporation into DNA. Tritium 219-221 cyclin G1 Rattus norvegicus 40-49 9252021-7 1997 The ratio of [3H]DA and [3H]NE in the spleen loaded with [3H]DA was found to be dependent on both temperature and time of loading, and could be modulated by various drugs such as desmethylimipramine, a NE uptake blocker, and disulfiram or fusaric acid, dopamine beta-hydroxylase inhibitors. Tritium 14-16 dopamine beta-hydroxylase Rattus norvegicus 253-278 9252021-7 1997 The ratio of [3H]DA and [3H]NE in the spleen loaded with [3H]DA was found to be dependent on both temperature and time of loading, and could be modulated by various drugs such as desmethylimipramine, a NE uptake blocker, and disulfiram or fusaric acid, dopamine beta-hydroxylase inhibitors. Tritium 25-27 dopamine beta-hydroxylase Rattus norvegicus 253-278 9440134-5 1997 In COS-7 cells transfected with rat EP2 cDNA, specific [3H]PGE2 binding was found with a dissociation constant of 14.9 nM, and this binding was inhibited by unlabeled PGE2 and PGE2 alpha. Tritium 56-58 prostaglandin E receptor 2 Rattus norvegicus 36-39 9276089-4 1997 The area of alkaline phosphatase-positive staining was increased twofold (p = 0.0008) and the number of [3H]thymidine-positive cells was increased twofold (p = 0.0024) in LIF-treated bones. Tritium 105-107 LIF interleukin 6 family cytokine Homo sapiens 171-174 9313951-11 1997 It is concluded that in human lymphocyte membranes [3H]-SCH 58261 directly labels binding sites showing the characteristic properties of the adenosine A2A-receptor. Tritium 52-54 adenosine A2a receptor Homo sapiens 141-163 9390776-2 1997 [3H]Ro41-1049 and [3H]lazabemide were used as selective radioligands to image and quantify MAO-A and MAO-B respectively by enzyme autoradiography. Tritium 1-3 monoamine oxidase A Homo sapiens 91-96 9277444-7 1997 IFN-gamma (300 U/ml), TNF-alpha (20 ng/ml), and IL-1 alpha (20 ng/ml) all induced a significantly increased release of prelabeled [3H]AA after 15 min to 2 h of treatment in control cells, and their effects were significantly reduced in cells transfected with cPLA2 antisense vector. Tritium 131-133 interleukin 1 alpha Homo sapiens 48-58 9252021-7 1997 The ratio of [3H]DA and [3H]NE in the spleen loaded with [3H]DA was found to be dependent on both temperature and time of loading, and could be modulated by various drugs such as desmethylimipramine, a NE uptake blocker, and disulfiram or fusaric acid, dopamine beta-hydroxylase inhibitors. Tritium 25-27 dopamine beta-hydroxylase Rattus norvegicus 253-278 2789227-4 1989 The results showed that pTGF-beta 1 and 2 decreased 3H-thymidine incorporation in CTLL cells in a dose-dependent fashion (maximum decrease of 75-85%). Tritium 52-54 hemoglobin, beta adult major chain Mus musculus 29-41 9249592-7 1997 In addition, Gent increased PLA2 activity (measured as [3H]arachiodonic acid release, 29,849 +/- 2,151 vs. 20,104 +/- 2,308 cpm/well in basal conditions), an effect that was blocked by AA (11,804 +/- 684 cpm/well). Tritium 56-58 phospholipase A2 group IB Rattus norvegicus 28-32 9283705-10 1997 Challenge of cultures with alpha, beta-meATP rapidly and concentration-dependently increased [3H]-arachidonic acid (AA) release from cells, suggesting that activation of phospholipase A2 (PLA2) may be involved in the long-term functional effects evoked by purine analogues. Tritium 94-96 phospholipase A2 group IB Rattus norvegicus 170-186 9283705-10 1997 Challenge of cultures with alpha, beta-meATP rapidly and concentration-dependently increased [3H]-arachidonic acid (AA) release from cells, suggesting that activation of phospholipase A2 (PLA2) may be involved in the long-term functional effects evoked by purine analogues. Tritium 94-96 phospholipase A2 group IB Rattus norvegicus 188-192 9283705-12 1997 Moreover, various PLA2 inhibitors (e.g. mepacrine and dexamethasone) prevented both the early alpha, beta-meATP-induced [3H]-AA release and/or the associated long-term morphological changes, without affecting the astrocytic elongation induced by bFGF. Tritium 121-123 phospholipase A2 group IB Rattus norvegicus 18-22 2548889-2 1989 The gamma-aminobutyric acid-benzodiazepine receptor (GABA-BZR)- and muscarinic cholinergic receptor (MChR)-binding sites in layer I of PC were localized using [3H]flunitrazepam and [3H]quinuclidinyl benzilate as ligands, respectively. Tritium 160-162 melanin-concentrating hormone receptor 1 Rattus norvegicus 101-105 9292231-8 1997 Characterization of RyR functional behaviour with [3H]-ryanodine has indicated extensive similarities between the enriched JFM and parent TC vessicles, as far as the characteristic bell shaped Ca(2+)-dependence of [3H]-ryanodine binding and the dose-dependent sensitization to Ca2+ by caffeine, reflecting the inherent properties of SR Ca(2+)-release channel, as well as concerning the stimulation of [3H]-ryanodine binding by increasing concentrations of KCl. Tritium 51-53 LOC100009439 Oryctolagus cuniculus 20-23 9292231-11 1997 Additional results with enriched JFM using doxorubicin, a pharmacological Ca2+ channel allosteric modifier, strengthen the hypothesis that the conformational state at which RyR is stabilized, according to the experimental assay conditions for [3H]-ryanodine binding, directly influences CaM-sensitivity. Tritium 244-246 LOC100009439 Oryctolagus cuniculus 173-176 9302360-4 1997 The effect on growth following addition of the peptides and the ligands for NPR-C was measured by [3H]thymidine incorporation. Tritium 99-101 natriuretic peptide receptor 3 Rattus norvegicus 76-81 9231818-7 1997 Interleukin-1 alpha treatment enhanced Ang II-induced [3H]uridine incorporation, whereas treatment with interleukin-1 alpha combined with tumor necrosis factor-alpha/interferon gamma attenuated Ang II-induced [3H]uridine and [3H]leucine incorporation. Tritium 210-212 interferon gamma Rattus norvegicus 166-182 2548889-2 1989 The gamma-aminobutyric acid-benzodiazepine receptor (GABA-BZR)- and muscarinic cholinergic receptor (MChR)-binding sites in layer I of PC were localized using [3H]flunitrazepam and [3H]quinuclidinyl benzilate as ligands, respectively. Tritium 160-163 melanin-concentrating hormone receptor 1 Rattus norvegicus 101-105 9215831-3 1997 Thymidylate synthase (TS) activity was measured according to the tritium-release assay. Tritium 65-72 thymidylate synthetase Homo sapiens 0-20 2680916-4 1989 As measured in a [3H]thymidine uptake assay, the normal peripheral blood B cells demonstrated a dose-dependent proliferative response to rIL-2, whereas the NHL cells did not show any responsiveness to rIL-2. Tritium 18-20 interleukin 2 Rattus norvegicus 137-142 9211345-4 1997 VDR expression was determined by RT-PCR generating a 297 bp fragment and by binding assays (Scatchard analysis) with [3H]-1,25(OH)2D3. Tritium 118-120 vitamin D receptor Rattus norvegicus 0-3 9283824-12 1997 BMP 6 and BMP 7 also increased the uptake of [3H]dopamine without affecting the uptake of [3H]5-hydroxytryptamine and [3H]GABA, underscoring the specificity of the trophic effect. Tritium 46-48 bone morphogenetic protein 7 Rattus norvegicus 10-15 9252980-7 1997 Species-specific Il-2 induced intracellular signals in AtT-20 cells known to be mediated by Il-2R beta, including a transient increase in c-myc nuclear proto-oncogene transcription and the dose-dependent induction of DNA replication as measured by [3H]thymidine incorporation. Tritium 249-251 interleukin 2 Mus musculus 17-21 9252534-7 1997 LPA and EGF, when added together, exhibited a markedly synergistic stimulation of [3H]thymidine incorporation that was typically 10-fold greater than the stimulation with either agent alone. Tritium 83-85 epidermal growth factor Homo sapiens 8-11 9247711-5 1997 The PC-SPES-modulated PSA changes occurred concurrently with the decrease of AR expression, based on Western blot analysis and binding to the radioactive ligand [3H]R1881. Tritium 162-164 kallikrein related peptidase 3 Homo sapiens 22-25 9248219-4 1997 The [3H]leucine incorporation in HUVECs increased when the cells were treated with crocidolite asbestos or man-made mineral fibers (MMMFs), indicating that the increases in TM activity and the TM antigen level on HUVECs directly exposed to those fibers may not reflect the sole induction of anticoagulant activities, but the general cell damage induced by the fibers. Tritium 5-7 thrombomodulin Homo sapiens 193-195 9228201-4 1997 On the other hand, when using [3H]SR 48968, NKA bound to a high affinity site, [beta-Ala8]NKA(4-10) bound to a low affinity site and NKA(4-10) and GR 64349 bound to a high and a low affinity site; in this case, the high affinity site was no longer detected in the presence of 1 microM GppNHp, which did not reduce the specific binding of [3H]SR 48968 to NK2 receptors. Tritium 31-33 Natural killer alloreactivity QTL 1 Rattus norvegicus 44-47 9219895-3 1997 PAF accumulation was accompanied by the activation of the CoA-independent transacylase and acetyl-CoA: lyso-PAF acetyltransferase, whereas the release of free [3H]arachidonic acid in prelabeled cells reflecting the activation of phospholipase A2, occurred primarily within the initial 1-5 min of treatment with LPS. Tritium 160-163 PCNA clamp associated factor Homo sapiens 0-3 9208130-16 1997 This rank order of potency of agonist BK-related peptides, coupled with the antagonism of the BK-induced [3H]-IPs by the specific B2-receptor antagonists, strongly suggests that a B2-receptor subtype is involved in mediating functional phosphoinositide (PI) responses in the CEPI-17-CL4 and P-CEPI cells. Tritium 106-108 bradykinin receptor B2 Homo sapiens 130-141 9208130-16 1997 This rank order of potency of agonist BK-related peptides, coupled with the antagonism of the BK-induced [3H]-IPs by the specific B2-receptor antagonists, strongly suggests that a B2-receptor subtype is involved in mediating functional phosphoinositide (PI) responses in the CEPI-17-CL4 and P-CEPI cells. Tritium 106-108 bradykinin receptor B2 Homo sapiens 180-191 9225308-1 1997 The NMB human neuronal cell line, transfected with a newly prepared plasmid expressing rat serotonin transporter (NMB-rSERT), shows specific [3H]5-HT uptake which is blocked by citalopram and fenfluramine (F) stereoisomers with IC50 values (1 nM. Tritium 142-144 neuromedin B Rattus norvegicus 114-117 9228201-4 1997 On the other hand, when using [3H]SR 48968, NKA bound to a high affinity site, [beta-Ala8]NKA(4-10) bound to a low affinity site and NKA(4-10) and GR 64349 bound to a high and a low affinity site; in this case, the high affinity site was no longer detected in the presence of 1 microM GppNHp, which did not reduce the specific binding of [3H]SR 48968 to NK2 receptors. Tritium 31-33 Natural killer alloreactivity QTL 1 Rattus norvegicus 90-93 9228201-4 1997 On the other hand, when using [3H]SR 48968, NKA bound to a high affinity site, [beta-Ala8]NKA(4-10) bound to a low affinity site and NKA(4-10) and GR 64349 bound to a high and a low affinity site; in this case, the high affinity site was no longer detected in the presence of 1 microM GppNHp, which did not reduce the specific binding of [3H]SR 48968 to NK2 receptors. Tritium 31-33 Natural killer alloreactivity QTL 1 Rattus norvegicus 90-93 9140562-3 1997 PROCEDURE: Triamcinolone acetonide and IGF-1 effects were evaluated by assessing: matrix responses by sulfated glycosaminoglycan (GAG) assay and [35S]sulfated GAG synthesis; collagen content by hydroxyproline assay; and mitogenic response by [3H]thymidine incorporation into DNA and fluorometric assay of total DNA concentration. Tritium 243-245 insulin like growth factor 1 Equus caballus 39-44 2588913-7 1989 The modulation or down-regulation of EGF receptors by PMA in 7402 cells was paralleled by the simultaneous inhibition of DNA synthesis in these cells as evidenced from their reduction of 3H-TdR uptake. Tritium 187-189 epidermal growth factor Homo sapiens 37-40 9157961-5 1997 [3H]PAF bound to adherent human macrophages with a K(d) of 2.1 nmol/L and a B(max) of 19 fmol/10(6) cells; approximately 5300 binding sites per cell were detected. Tritium 1-3 PCNA clamp associated factor Homo sapiens 4-7 9195910-5 1997 The 10-min uptake of [3H]glycerol was increased significantly (range of 4.5-8-fold over control) in oocytes expressing untagged AQP3 (GLIP) and each of the four tagged AQP3 constructs; [3H]glycerol uptake was not increased in oocytes expressing AQP1, AQP2, AQP4, AQP5, or MIP. Tritium 22-24 aquaporin 1 (Colton blood group) L homeolog Xenopus laevis 245-249 9218635-2 1997 A GTP analogue guanylyl-imidodiphosphate (GMP-PNP) at 100 microM markedly increased the K(d) values of the [3H]NPA binding sites in the rat forebrain sections, and coincubation with CCK-8 (1 nM) again did not produce a further increase in the K(d) values. Tritium 108-110 purine nucleoside phosphorylase Rattus norvegicus 46-49 2546501-8 1989 Ketoconazole lengthened the time to reach peak VDR up-regulation to 20 h. The ability of ketoconazole to increase cell responsiveness (VDR up-regulation) was the result of both increased and prolonged occupancy of VDR by 1,25-[3H]D3. Tritium 227-229 vitamin D receptor Rattus norvegicus 135-138 9152998-0 1997 [3H]WAY-100635 for 5-HT1A receptor autoradiography in human brain: a comparison with [3H]8-OH-DPAT and demonstration of increased binding in the frontal cortex in schizophrenia. Tritium 1-3 5-hydroxytryptamine receptor 1A Homo sapiens 19-34 9152998-7 1997 Using [3H]WAY-100635, we confirmed an increase of 5-HT1A receptor binding sites in the frontal cortex in schizophrenia, previously demonstrated with [3H]8-OH-DPAT. Tritium 7-9 5-hydroxytryptamine receptor 1A Homo sapiens 50-65 9152998-7 1997 Using [3H]WAY-100635, we confirmed an increase of 5-HT1A receptor binding sites in the frontal cortex in schizophrenia, previously demonstrated with [3H]8-OH-DPAT. Tritium 150-152 5-hydroxytryptamine receptor 1A Homo sapiens 50-65 9101422-5 1997 Experiments with [3H]lyso PAF indicate that human mononuclear bone marrow cells and marrow stromal cells actively acylate lyso PAF into a 1-alkyl analogue of phosphatidylcholine. Tritium 18-21 PCNA clamp associated factor Homo sapiens 127-130 9160247-5 1997 The rank order potencies of the examined compounds revealed that the opioid component of [3H]Met-enkephalin-Arg6-Phe7 recognition site are probably not mu and certainly not kappa 1 sites, whereas these sites are characterized by a kappa 2-like binding profile. Tritium 90-92 proenkephalin Rattus norvegicus 97-107 9160247-8 1997 [3H]Met-enkephalin-Arg6-Phe7 binding can also be inhibited by FMRF-amide analogs and sigma receptor ligands, such as (+)N-allyl-normetazocine and haloperidol, although with moderate affinity. Tritium 1-3 proenkephalin Rattus norvegicus 8-18 9152998-8 1997 Compared to [3H]8-OH-DPAT, [3H]WAY-100635 has two advantages: it has a higher selectivity and affinity for the 5-HT1A receptor, and it recognizes 5-HT1A receptors whether or not they are coupled to a G-protein, whereas [3H]8-OH-DPAT primarily detects coupled receptors. Tritium 28-30 5-hydroxytryptamine receptor 1A Homo sapiens 111-126 9152998-8 1997 Compared to [3H]8-OH-DPAT, [3H]WAY-100635 has two advantages: it has a higher selectivity and affinity for the 5-HT1A receptor, and it recognizes 5-HT1A receptors whether or not they are coupled to a G-protein, whereas [3H]8-OH-DPAT primarily detects coupled receptors. Tritium 28-30 5-hydroxytryptamine receptor 1A Homo sapiens 111-117 9096877-5 1997 Pulse labeling of FSH-pretreated cells with [3H]pregnenolone revealed relatively rapid (< 5 h) transformation to [3H]progesterone and other distal products that was accelerated by the concurrent addition of IGF-I. Tritium 45-47 insulin-like growth factor 1 Rattus norvegicus 210-215 9096877-5 1997 Pulse labeling of FSH-pretreated cells with [3H]pregnenolone revealed relatively rapid (< 5 h) transformation to [3H]progesterone and other distal products that was accelerated by the concurrent addition of IGF-I. Tritium 117-119 insulin-like growth factor 1 Rattus norvegicus 210-215 9812858-4 1997 It was found that: (1) IL-2 (10-500 U/ml) significantly stimulated 3H-TdR incorporation into AP cells of female pregnant as well as none-pregnant rats, but inhibited 3H-TdR incorporation in the male rats. Tritium 67-69 interleukin 2 Rattus norvegicus 23-27 9152998-8 1997 Compared to [3H]8-OH-DPAT, [3H]WAY-100635 has two advantages: it has a higher selectivity and affinity for the 5-HT1A receptor, and it recognizes 5-HT1A receptors whether or not they are coupled to a G-protein, whereas [3H]8-OH-DPAT primarily detects coupled receptors. Tritium 28-30 5-hydroxytryptamine receptor 1A Homo sapiens 111-126 2546501-8 1989 Ketoconazole lengthened the time to reach peak VDR up-regulation to 20 h. The ability of ketoconazole to increase cell responsiveness (VDR up-regulation) was the result of both increased and prolonged occupancy of VDR by 1,25-[3H]D3. Tritium 227-229 vitamin D receptor Rattus norvegicus 135-138 9152998-8 1997 Compared to [3H]8-OH-DPAT, [3H]WAY-100635 has two advantages: it has a higher selectivity and affinity for the 5-HT1A receptor, and it recognizes 5-HT1A receptors whether or not they are coupled to a G-protein, whereas [3H]8-OH-DPAT primarily detects coupled receptors. Tritium 28-30 5-hydroxytryptamine receptor 1A Homo sapiens 111-117 9152998-10 1997 First, they indicate that the elevated [3H]8-OH-DPAT binding seen in the same cases is attributable to an increase of 5-HT1A receptors rather than any other binding site. Tritium 40-42 5-hydroxytryptamine receptor 1A Homo sapiens 118-124 9812858-4 1997 It was found that: (1) IL-2 (10-500 U/ml) significantly stimulated 3H-TdR incorporation into AP cells of female pregnant as well as none-pregnant rats, but inhibited 3H-TdR incorporation in the male rats. Tritium 166-168 interleukin 2 Rattus norvegicus 23-27 2665849-4 1989 Most likely, IL-1 exerted these stimulative effects directly on AML blast cells because IL-1 effectively induced 3H-TdR uptake of CD34-positive AML blasts (separated following cell sorting). Tritium 113-115 interleukin 1 alpha Homo sapiens 13-17 9152998-11 1997 Second, the enhanced [3H]8-OH-DPAT binding in schizophrenia reflects an increased density of 5-HT1A receptors, not an increased percentage of 5-HT1A receptors which are G-protein-coupled. Tritium 22-24 5-hydroxytryptamine receptor 1A Homo sapiens 93-99 9152998-12 1997 We conclude that [3H]WAY-100635 is a valuable autoradiographic ligand for the qualitative and quantitative study of 5-HT1A receptors in the human brain. Tritium 18-20 5-hydroxytryptamine receptor 1A Homo sapiens 116-122 2665849-4 1989 Most likely, IL-1 exerted these stimulative effects directly on AML blast cells because IL-1 effectively induced 3H-TdR uptake of CD34-positive AML blasts (separated following cell sorting). Tritium 113-115 interleukin 1 alpha Homo sapiens 88-92 9225308-3 1997 d-Fenfluramine (0.5 and 10 microM) also stimulates tritium release from NMB-rSERT cells preloaded with [3H-]-5-HT. Tritium 51-58 neuromedin B Rattus norvegicus 72-75 9054420-7 1997 This molecule was also able to inhibit [3H]suramin binding to recombinant gp120 with similar activity. Tritium 40-42 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 74-79 2503386-7 1989 The culture supernatant from spleen cells of recovered mice (TrSN) contains high levels of macrophage-activating factor (MAF) activity which can activate peritoneal macrophages to kill 51Cr-labeled P815 cells and to eliminate intracellular parasites as measured by the reduction in [3H]thymidine uptake by residual parasites released from macrophages following sodium dodecyl sulfate treatment. Tritium 283-285 avian musculoaponeurotic fibrosarcoma oncogene homolog Mus musculus 91-119 9084507-6 1997 Exogenous group II phospholipase A2 (5-36 U/ml) purified homogeneously from rat spleen also increased [3H]thymidine incorporation by platelet derived growth factor-stimulated mesangial cells in a dose dependent manner (36 U/ml phospholipase A2; 1.9-fold). Tritium 103-105 phospholipase A2 group IB Rattus norvegicus 19-35 9225308-3 1997 d-Fenfluramine (0.5 and 10 microM) also stimulates tritium release from NMB-rSERT cells preloaded with [3H-]-5-HT. Tritium 104-106 neuromedin B Rattus norvegicus 72-75 9225308-5 1997 Manipulation of the Na+ gradient across the plasma membrane (replacing 60 mM NaCl with an equimolar concentration of KCl or choline) also induced [3H-]5-HT release from NMB-rSERT cells, which was inhibited by 0.3 microM citalopram. Tritium 147-149 neuromedin B Rattus norvegicus 169-172 2503386-7 1989 The culture supernatant from spleen cells of recovered mice (TrSN) contains high levels of macrophage-activating factor (MAF) activity which can activate peritoneal macrophages to kill 51Cr-labeled P815 cells and to eliminate intracellular parasites as measured by the reduction in [3H]thymidine uptake by residual parasites released from macrophages following sodium dodecyl sulfate treatment. Tritium 283-285 avian musculoaponeurotic fibrosarcoma oncogene homolog Mus musculus 121-124 9166787-8 1997 Reciprocal substitution in FR-beta with peptide 1-92 of FR-alpha resulted in poor expression of a [3H]folic acid binding protein. Tritium 99-101 folate receptor beta Homo sapiens 27-34 9074411-7 1997 Leukaemic cells derived from seven (four acute-type, one chronic-type and two lymphoma-type) of the 14 patients proliferated in vitro in response to G-CSF, as measured by [3H]thymidine incorporation; maximum responses were at G-CSF concentrations of 10-100 ng/ml. Tritium 172-174 colony stimulating factor 3 Homo sapiens 149-154 2570849-1 1989 p-Bromophenacyl bromide (PBPB), quinacrine and indomethacin, which inhibit phospholipase A2 (PLA2; EC 3.1.1.4) activity in several tissues, caused a dose-dependent inhibition of prelabelled [3H]noradrenaline ([3H]NA) release evoked by high concentrations of K+ from rat cerebral cortical synaptosomes. Tritium 191-193 phospholipase A2 group IB Rattus norvegicus 75-91 9042336-7 1997 We also found that genistein and methyl 2,5-dihydroxycinnamate decreased both basal and PAF-stimulated [3H]thymidine uptake in these cells. Tritium 104-106 PCNA clamp associated factor Homo sapiens 88-91 9042336-9 1997 PAF-stimulated [3H]thymidine uptake was also prevented by PKC down regulation after long term exposure to PMA and PKC inhibition with the two inhibitors sangivamycin and bis-indolylmaleimide. Tritium 16-18 PCNA clamp associated factor Homo sapiens 0-3 9166787-8 1997 Reciprocal substitution in FR-beta with peptide 1-92 of FR-alpha resulted in poor expression of a [3H]folic acid binding protein. Tritium 99-101 rabaptin, RAB GTPase binding effector protein 2 Homo sapiens 56-64 9166787-9 1997 By analysis of chimeric constructs, the poor [3H]folic acid binding of the FR-alpha(1-92)/beta(93-237) chimera could be attributed to interference of a short segment from FR-alpha in the vicinity of Ala 49 (peptide 39-59) with proper folding of the chimera. Tritium 46-48 rabaptin, RAB GTPase binding effector protein 2 Homo sapiens 75-83 9213209-5 1997 NPY, its endogenous analog, peptide YY, and its C-terminal fragment, NPY13-36, but not its analog, [Leu31,Pro34]NPY, concentration dependently (1-100 nM) inhibited [3H]noradrenaline release in all tissues studied. Tritium 165-167 neuropeptide Y Homo sapiens 0-3 9213209-5 1997 NPY, its endogenous analog, peptide YY, and its C-terminal fragment, NPY13-36, but not its analog, [Leu31,Pro34]NPY, concentration dependently (1-100 nM) inhibited [3H]noradrenaline release in all tissues studied. Tritium 165-167 neuropeptide Y Homo sapiens 69-72 9178195-5 1997 When subsequently studied in an in-vitro model for [3H]PAF binding in neutrophils from man the compound caused dose-dependent displacement of [3H]PAF from its receptor with an IC50 value of 2 microM. Tritium 52-54 PCNA clamp associated factor Homo sapiens 55-58 9178195-5 1997 When subsequently studied in an in-vitro model for [3H]PAF binding in neutrophils from man the compound caused dose-dependent displacement of [3H]PAF from its receptor with an IC50 value of 2 microM. Tritium 52-54 PCNA clamp associated factor Homo sapiens 146-149 9178195-5 1997 When subsequently studied in an in-vitro model for [3H]PAF binding in neutrophils from man the compound caused dose-dependent displacement of [3H]PAF from its receptor with an IC50 value of 2 microM. Tritium 143-145 PCNA clamp associated factor Homo sapiens 55-58 9105961-1 1997 5-HT1A receptor binding sites were measured, by saturation binding with [3H]8-OH-DPAT, in frontal and occipital cortex, hippocampus and amygdala obtained at post-mortem examination from suicide victims with a firm retrospective diagnosis of depression, and matched controls. Tritium 73-75 5-hydroxytryptamine receptor 1A Homo sapiens 0-15 8988346-4 1997 At 24 h, the mice were sacrificed and the incorporation of [3H]proline into collagenase-digestible CDP labeling) and noncollagen (NCP labeling) protein in calvariae were determined by digestion with bacterial collagenase. Tritium 60-62 cut-like homeobox 1 Mus musculus 99-102 9007531-0 1996 Histamine H1 receptor activation stimulates [3H]GABA release from human astrocytoma U373 MG cells. Tritium 45-47 histamine receptor H1 Homo sapiens 0-21 9007531-6 1996 The effects of histamine on [3H]GABA release, [3H]inositol phosphate accumulation and Ca2+ mobilisation were blocked by the selective histamine H1 receptor antagonist mepyramine. Tritium 28-31 histamine receptor H1 Homo sapiens 134-155 9178195-5 1997 When subsequently studied in an in-vitro model for [3H]PAF binding in neutrophils from man the compound caused dose-dependent displacement of [3H]PAF from its receptor with an IC50 value of 2 microM. Tritium 143-145 PCNA clamp associated factor Homo sapiens 146-149 2570849-1 1989 p-Bromophenacyl bromide (PBPB), quinacrine and indomethacin, which inhibit phospholipase A2 (PLA2; EC 3.1.1.4) activity in several tissues, caused a dose-dependent inhibition of prelabelled [3H]noradrenaline ([3H]NA) release evoked by high concentrations of K+ from rat cerebral cortical synaptosomes. Tritium 191-193 phospholipase A2 group IB Rattus norvegicus 93-97 2668523-10 1989 The pattern of [3H]pregnenolone metabolism was measured after 5 days injection of 50 ng GnRH/day. Tritium 16-18 gonadotropin releasing hormone 1 Mus musculus 88-92 9166281-4 1997 These cells exhibited a dose-dependent proliferative response to rIL-10 (23% to 70% increases at 80 ng/mL; p < 0.01), as assessed by both 3H-thymidine uptake and cell count. Tritium 141-143 interleukin 10 Rattus norvegicus 65-71 9387387-4 1996 Hypoxia enhanced the increment in 3H-TdR incorporation induced by EGF. Tritium 34-36 epidermal growth factor Bos taurus 66-69 2789334-4 1989 Glycosylation of the EGF precursor was determined by (i) the direct incorporation of [3H]mannose and [3H]glucosamine, (ii) metabolic labeling in the presence or absence of glycosylation inhibitors, (iii) enzymatic cleavage of the precursor by N-glycanase or endoglycosidase II, and (iv) lectin chromatography. Tritium 86-88 epidermal growth factor Homo sapiens 21-24 8985683-5 1996 When NR1 receptor levels were assessed by receptor autoradiography ([3H]-MK 801 and -CGP 39653 binding) in the same groups of mice, significant (20%) reductions in NR1 levels were observed in the retrosplenial cortex and the overall cortex. Tritium 69-71 glutamate receptor, ionotropic, NMDA1 (zeta 1) Mus musculus 5-8 10072949-6 1997 Interleukin-1 (IL-1) and interleukin-2 (IL-2) activities were evaluated by [3H]TdR uptake. Tritium 76-78 interleukin 2 Rattus norvegicus 40-44 9139848-6 1997 On the other hand, cytolysis of tumor cells, as determined by a [3H]-uridine release assay, was first observed only at 24 hr of incubation with the rIFN- gamma-activated neutrophils. Tritium 65-67 interferon gamma Rattus norvegicus 148-159 2789334-4 1989 Glycosylation of the EGF precursor was determined by (i) the direct incorporation of [3H]mannose and [3H]glucosamine, (ii) metabolic labeling in the presence or absence of glycosylation inhibitors, (iii) enzymatic cleavage of the precursor by N-glycanase or endoglycosidase II, and (iv) lectin chromatography. Tritium 102-104 epidermal growth factor Homo sapiens 21-24 2768223-4 1989 From the chromatographic analysis of the cytosol incubated with [3H]bilirubin, it was found that a major part of the added bilirubin binds to subunit 1 (Ya) of GST isozyme, leaving not only the conjugation activity derived from 3-4 type GST but also the CHPx activity of subunit 2 (Yc) quantitatively intact. Tritium 65-67 hematopoietic prostaglandin D synthase Rattus norvegicus 160-163 9142906-7 1997 Hypoxia resulted in an elevation in the activity of the inducible Ca2+-independent isoform of NO synthase in colonic tissue, which was maximal between 4 and 6 h posthypoxia and was associated with an increase in myeloperoxidase activity, [3H]mannitol flux, luminal protein content, and histological damage. Tritium 239-241 myeloperoxidase Rattus norvegicus 212-227 8972543-0 1996 Autoradiographic localisation of D3-dopamine receptors in the human brain using the selective D3-dopamine receptor agonist (+)-[3H]PD 128907. Tritium 123-130 dopamine receptor D3 Homo sapiens 33-53 8972543-1 1996 The selective D3-dopamine receptor agonist 4aR, 10bR-(+)-trans-3,4,4a, 10b-tetrahydro-4-[N-propyl-2,3-3H]-2H,5H-[1] benzopyrano[4,3-b]-1,4-oxazin-9-ol ([3H]PD 128907) was used to visualise D3-dopamine receptors in whole hemisphere cryosections from post-mortem human brain. Tritium 102-104 dopamine receptor D3 Homo sapiens 14-34 8972543-6 1996 A very high proportion of [3H]PD 128907 was specifically bound, as judged from the low binding remaining in the presence of the D2/D3-dopamine receptor antagonist raclopride. Tritium 27-29 dopamine receptor D3 Homo sapiens 131-151 8972543-10 1996 The new compound [3H]PD 128907 appears to be a suitable radioligand for autoradiographic examination of the D3-dopamine receptor localisation in the human brain, and should also be useful for pharmacological studies of this receptor subtype. Tritium 18-20 dopamine receptor D3 Homo sapiens 108-128 9180390-7 1997 Addition of GnRH agonist (D-Lys6)GnRH to the cell cultures caused a time-dependent decrease in both cell growth, as measured by cell number, and cell proliferation, as measured by [3H]thymidine incorporation into DNA. Tritium 181-183 gonadotropin releasing hormone 1 Mus musculus 12-16 9180390-7 1997 Addition of GnRH agonist (D-Lys6)GnRH to the cell cultures caused a time-dependent decrease in both cell growth, as measured by cell number, and cell proliferation, as measured by [3H]thymidine incorporation into DNA. Tritium 181-183 gonadotropin releasing hormone 1 Mus musculus 33-37 2475050-2 1989 In the present study, the in vitro antitumor effect of K18 was assessed in a total of 107 fresh human tumors by DNA synthesis (3H-thymidine incorporation) inhibition assay, and compared with that of other drugs. Tritium 127-129 keratin 18 Homo sapiens 55-58 9180390-8 1997 After 1 h of treatment of alphaT3-1 cells with 1 microM of (D-Lys6)GnRH, the cell number was reduced to 83.0 +/- 13.4 compared to control, decreased to 75.1 +/- 3.2 at 2 h, 63.2 +/- 0.66 at 4 h and 52.2 +/- 0.87 at 24 h. This decrease in cell number was accompanied by a parallel decrease in [3H]thymidine incorporation into DNA. Tritium 293-295 gonadotropin releasing hormone 1 Mus musculus 67-71 9225276-7 1997 Guinea pig 5-HT1B and 5-HT1D receptor genes were transiently expressed in Cos-7 cells and their binding properties were evaluated using [3H]5-HT. Tritium 137-139 5-hydroxytryptamine receptor 1D Cavia porcellus 22-37 9065411-4 1997 In fact, a 30-min treatment of T67 cells with the combination of lipopolysaccharide plus interferon-gamma (MIX) strongly inhibits the cNOS activity, as determined by measuring [3H]citrulline production. Tritium 177-179 Mix paired-like homeobox Homo sapiens 107-110 9134216-5 1997 The expression of either P2Y1 or P2Y2 receptors in 1321N1 human astrocytoma cells results, on a change of medium, in an elevation of basal (no added agonist) accumulation of [3H]-inositol(poly)phosphates([3H]-InsPx) compared to cells not expressing these receptors. Tritium 175-177 purinergic receptor P2Y1 Homo sapiens 25-29 9134216-5 1997 The expression of either P2Y1 or P2Y2 receptors in 1321N1 human astrocytoma cells results, on a change of medium, in an elevation of basal (no added agonist) accumulation of [3H]-inositol(poly)phosphates([3H]-InsPx) compared to cells not expressing these receptors. Tritium 205-207 purinergic receptor P2Y1 Homo sapiens 25-29 9134216-8 1997 Both PPADS and suramin reduced this basal level of [3H]-InsPx accumulation in the P2Y1 expressing cells. Tritium 52-54 purinergic receptor P2Y1 Homo sapiens 82-86 9067309-4 1997 Uptake of [3H]DPDPE into the CSF could not be inhibited (K(d) = 0.9 +/- 0.1 microl x min(-1) x g(-1)). Tritium 11-13 colony stimulating factor 2 Homo sapiens 29-32 9027325-5 1996 Low doses of exogenous EGF stimulated 3H-thymidine incorporation, and monoclonal antibodies against EGF or EGFR dose-dependently inhibited 3H-thymidine incorporation. Tritium 38-40 epidermal growth factor Homo sapiens 23-26 9027325-5 1996 Low doses of exogenous EGF stimulated 3H-thymidine incorporation, and monoclonal antibodies against EGF or EGFR dose-dependently inhibited 3H-thymidine incorporation. Tritium 139-141 epidermal growth factor Homo sapiens 100-103 8910451-5 1996 The most potent egr-1 antisense ODN inhibited serum-induced egr-1 mRNA by 68%, protein induction by 58%, and MC replication as measured by [3H]thymidine uptake and cell counts by 78 and 46%, respectively. Tritium 140-142 early growth response 1 Homo sapiens 16-21 9020139-7 1997 When mixtures of the 32-amino acid basic peptide corresponding to the essential IPn binding region of the Syt II-C2B domain and GST-Syt II-C2B were labeled by a stoichiometric amount of P-2-linked [3H]BZDC-IP6, the two polypeptides showed equivalent affinity for the photolabel. Tritium 198-200 glutathione S-transferase kappa 1 Homo sapiens 128-131 8968727-9 1996 We have found that the distribution of 5-HT1A receptors in monkey brain, labelled with the agonist 3H-8-OH-DPAT and the antagonist 3H-WAY 100635 was very similar at the levels examined, and corresponded well with that observed for the cells containing mRNA coding for this receptor, confirming the somatodendritic localization of 5-HT1A receptors in monkey brain. Tritium 99-101 5-hydroxytryptamine receptor 1A Homo sapiens 39-45 2707155-4 1989 Insulin-like growth factor I (IGF-I) stimulated [3H]thymidine incorporation by preadipocytes and stromal cells. Tritium 49-51 insulin-like growth factor 1 Rattus norvegicus 0-28 8858921-6 1996 Stimulatory effects of insulin on [3H]-STX binding and veratridine-induced 22Na+ influx were nullified by simultaneous treatment with either 5,6-dichlorobenzimidazole riboside, an inhibitor of RNA synthesis, or cycloheximide, an inhibitor of protein synthesis, whereas insulin treatment did not appreciably increase the level of mRNA encoding the Na+ channel alpha-subunit. Tritium 35-37 insulin Bos taurus 23-30 9042331-7 1997 Binding of [3H]LY181984 to HeLa cells also was enhanced by EGF. Tritium 12-14 epidermal growth factor Homo sapiens 59-62 9042331-10 1997 However, if the LY181984 was followed by addition of 10 nM EGF, the EC50 for LY181984 was reduced to about 30 nM which now approximated the previously determined Kd of [3H]LY181984 binding of 30 nM and the EC50 of 30 nM for inhibition of NADH oxidase activity by LY181984 by isolated vesicles of plasma membranes. Tritium 169-171 epidermal growth factor Homo sapiens 59-62 9042332-4 1997 Exposure of the LIF stimulated BEAS 2B cells to calcium ionophore A23187 (10(-5) M, 15 min) caused a further increase of [3H]AA release. Tritium 122-124 LIF interleukin 6 family cytokine Homo sapiens 16-19 8858930-4 1996 PAF increased the production of 3H-labeled inositol phosphates (IPs) with EC50 values of 1.2-1.5 nM. Tritium 32-34 PCNA clamp associated factor Homo sapiens 0-3 2707155-4 1989 Insulin-like growth factor I (IGF-I) stimulated [3H]thymidine incorporation by preadipocytes and stromal cells. Tritium 49-51 insulin-like growth factor 1 Rattus norvegicus 30-35 8858930-5 1996 The effect of PAF on 3H-IPs formation was completely blocked by the PAF antagonist BN 50739 at a concentration of 300 nM. Tritium 21-23 PCNA clamp associated factor Homo sapiens 14-17 2707157-4 1989 PP12 produced dose-dependent inhibition of IGF-I-stimulated [3H]thymidine incorporation in the rat thyroid follicular cell line FRTL5. Tritium 61-63 insulin-like growth factor binding protein 1 Rattus norvegicus 0-4 8858930-5 1996 The effect of PAF on 3H-IPs formation was completely blocked by the PAF antagonist BN 50739 at a concentration of 300 nM. Tritium 21-23 PCNA clamp associated factor Homo sapiens 68-71 9124287-2 1997 PLA2 activity was measured in isolated membrane and cytosol fractions with (16:0,[3H]18:1) plasmenylcholine and (16:0,[3H]18:1) phosphatidylcholine in the absence and presence of Ca2+. Tritium 82-84 phospholipase A2 group IB Rattus norvegicus 0-4 9124287-2 1997 PLA2 activity was measured in isolated membrane and cytosol fractions with (16:0,[3H]18:1) plasmenylcholine and (16:0,[3H]18:1) phosphatidylcholine in the absence and presence of Ca2+. Tritium 119-121 phospholipase A2 group IB Rattus norvegicus 0-4 2707157-4 1989 PP12 produced dose-dependent inhibition of IGF-I-stimulated [3H]thymidine incorporation in the rat thyroid follicular cell line FRTL5. Tritium 61-63 insulin-like growth factor 1 Rattus norvegicus 43-48 9124304-13 1997 Together, these results strongly suggest that bastadins, through their modulatory actions on the FKBP12-Ry1R complex, convert ryanodine-insensitive leak states into ryanodine-sensitive channels that recognize [3H]ryanodine with high affinity. Tritium 210-212 FK506 binding protein 1a Mus musculus 97-103 8858930-10 1996 Moreover, specific [3H]PAF binding sites coprecipitated with G alpha q and G alpha i1/2 proteins. Tritium 20-22 PCNA clamp associated factor Homo sapiens 23-26 2618542-4 1989 On the other hand the in vitro 3H-TdR uptake by the myeloma cells increased markedly with the decrease in the M-protein secretion rate. Tritium 31-33 myomesin 2 Homo sapiens 110-119 8906588-10 1996 [3H]cholesterol uptake by neonatal rabbit aortic smooth muscle and HepG2 cells was enhanced by a synthetic peptide corresponding to amino acids 1-18 of hSAA1, but not by peptides corresponding to amino acids 1-18 of hSAA4. Tritium 1-3 serum amyloid A4, constitutive Homo sapiens 216-221 9028532-5 1997 Stimulation of proliferation as determined by [3H]-thymidine incorporation was observed for these cells in response to fetal bovine serum, platelet derived growth factor, and transforming growth factor beta-1. Tritium 47-49 myotrophin Rattus norvegicus 156-169 2705543-7 1989 Cells prelabeled with [3H]PAF released some [3H]PAF within minutes and then rapidly converted it to lyso-PAF extracellularly. Tritium 23-25 PCNA clamp associated factor Homo sapiens 26-29 9028532-5 1997 Stimulation of proliferation as determined by [3H]-thymidine incorporation was observed for these cells in response to fetal bovine serum, platelet derived growth factor, and transforming growth factor beta-1. Tritium 47-49 myotrophin Rattus norvegicus 188-201 9040486-5 1997 In situ hybridization was performed on paraffin sections using a tritium-labeled probe for cathepsin G or alpha 1-PI. Tritium 65-72 cathepsin G Homo sapiens 91-102 9023314-6 1997 The presence of a LiCI-sensitive inositol polyphosphate 1-phosphatase (EC 3.1.3.57) was demonstrated, and high LiCl concentrations (30 mM) caused a significant enhancement of [3H]Ins(1,4)P2 accumulation and a corresponding decline in [3H]Ins4P levels. Tritium 176-178 inositol polyphosphate-1-phosphatase Bos taurus 33-69 9023314-6 1997 The presence of a LiCI-sensitive inositol polyphosphate 1-phosphatase (EC 3.1.3.57) was demonstrated, and high LiCl concentrations (30 mM) caused a significant enhancement of [3H]Ins(1,4)P2 accumulation and a corresponding decline in [3H]Ins4P levels. Tritium 235-237 inositol polyphosphate-1-phosphatase Bos taurus 33-69 8891693-2 1996 In this study we investigated the effects of insulin, insulin-like growth factor I and II (IGF-I and IGF-II), IGF-I analogues, and factors that interfere with their respective receptors, on [3H]thymidine incorporation into cultured nerve segments from the rat sciatic nerve. Tritium 191-193 insulin-like growth factor 1 Rattus norvegicus 101-106 8891693-3 1996 Segments cultured in nM (0.1-1.7 nM) concentrations of insulin, truncated IGF-I (tIGF-I), long R3IGF-I, or IGF-II exhibited an increase in [3H]thymidine incorporation compared with control segments. Tritium 140-142 insulin-like growth factor 1 Rattus norvegicus 74-79 8894651-4 1996 HG myoblasts were also more responsive to insulin-like growth factor-I (IGF-I) in terms of [3H]-thymidine incorporation. Tritium 92-94 insulin like growth factor 1 Gallus gallus 42-70 8894651-4 1996 HG myoblasts were also more responsive to insulin-like growth factor-I (IGF-I) in terms of [3H]-thymidine incorporation. Tritium 92-94 insulin like growth factor 1 Gallus gallus 72-77 2705543-7 1989 Cells prelabeled with [3H]PAF released some [3H]PAF within minutes and then rapidly converted it to lyso-PAF extracellularly. Tritium 23-25 PCNA clamp associated factor Homo sapiens 48-51 2705543-7 1989 Cells prelabeled with [3H]PAF released some [3H]PAF within minutes and then rapidly converted it to lyso-PAF extracellularly. Tritium 23-25 PCNA clamp associated factor Homo sapiens 48-51 8757253-2 1996 GAT1 was expressed in Xenopus oocytes and studied electrophysiologically as well as with [3H]GABA flux; GAT1 was also expressed in mammalian cells and studied with [3H]GABA and [3H]tiagabine binding. Tritium 90-92 solute carrier family 6 (neurotransmitter transporter), member 1 S homeolog Xenopus laevis 0-4 9039820-6 1997 Food restriction-induced GST activity assayed in an in vitro system, using [3H]AFB1-8,9-epoxide and glutathione (GSH) as substrates, was also found when mouse kidney and lung cytosolic fractions were used. Tritium 76-78 hematopoietic prostaglandin D synthase Rattus norvegicus 25-28 2705543-7 1989 Cells prelabeled with [3H]PAF released some [3H]PAF within minutes and then rapidly converted it to lyso-PAF extracellularly. Tritium 45-47 PCNA clamp associated factor Homo sapiens 26-29 2705543-7 1989 Cells prelabeled with [3H]PAF released some [3H]PAF within minutes and then rapidly converted it to lyso-PAF extracellularly. Tritium 45-47 PCNA clamp associated factor Homo sapiens 48-51 2705543-7 1989 Cells prelabeled with [3H]PAF released some [3H]PAF within minutes and then rapidly converted it to lyso-PAF extracellularly. Tritium 45-47 PCNA clamp associated factor Homo sapiens 48-51 9015795-11 1997 For example, clozapine revealed a radioligand-independent value of 1.6 nM at the dopamine D4 receptor, agreeing with the value directly measured with [3H]-clozapine at D4. Tritium 151-153 dopamine receptor D4 Homo sapiens 81-101 8794891-0 1996 Studies on alpha v beta 3/ligand interactions using a [3H]SK&F-107260 binding assay. Tritium 55-57 eukaryotic translation elongation factor 1 beta 2 pseudogene 2 Homo sapiens 11-25 2764978-5 1989 The treatment of the 3H and 14C labeled peptidyl-tRNA complexes with alpha-N-acetylgalactosaminidase led to the release of radiolabeled N-acetylgalactosamine, whereas alkaline borohydride reduction produced N-acetylgalactosaminitol. Tritium 21-23 alpha-N-acetylgalactosaminidase Rattus norvegicus 69-100 8794891-2 1996 A solid-phase microtiter assay was developed to investigate the binding properties of purified alpha v beta 3, using tritiated [3H]SK&F-107260 as the radiolabeled ligand. Tritium 128-130 eukaryotic translation elongation factor 1 beta 2 pseudogene 2 Homo sapiens 95-109 8794891-3 1996 alpha v beta 3, purified from human platelets, human placenta, and chicken osteoclasts, bound [3H]SK&F-107260 saturably and specifically. Tritium 95-97 eukaryotic translation elongation factor 1 beta 2 pseudogene 2 Homo sapiens 8-14 8794891-4 1996 Saturation binding studies using platelet alpha v beta 3 revealed a single class of high affinity binding sites, exhibiting a Kd of 1.44 nM and Bmax of 0.20 mol of [3H]SK&F-107260/mol of alpha v beta 3. Tritium 165-167 eukaryotic translation elongation factor 1 beta 2 pseudogene 2 Homo sapiens 50-56 8794891-4 1996 Saturation binding studies using platelet alpha v beta 3 revealed a single class of high affinity binding sites, exhibiting a Kd of 1.44 nM and Bmax of 0.20 mol of [3H]SK&F-107260/mol of alpha v beta 3. Tritium 165-167 eukaryotic translation elongation factor 1 beta 2 pseudogene 2 Homo sapiens 42-56 8794891-5 1996 [3H]SK&F-107260 binding was inhibited by a variety of RGD-containing peptides and by the snake venom protein echistatin, whereas an RGE-containing peptide and four nonpeptide fibrinogen receptor (alpha IIb beta 3) antagonists failed to do so. Tritium 1-3 eukaryotic translation elongation factor 1 beta 2 pseudogene 2 Homo sapiens 210-216 8794891-10 1996 The rank order of potency of the antagonists in the cell adhesion assays was similar to that of the receptor binding assay, suggesting that the purified alpha v beta 3-[3H]SK&F-107260 binding assay is a valid reflection of the ligand binding to alpha v beta 3 on cell systems. Tritium 169-171 eukaryotic translation elongation factor 1 beta 2 pseudogene 2 Homo sapiens 153-167 9672607-4 1997 [3H]-fucose labelled gp 120 was subjected to endoglycosidase F digestion, releasing diantennary complex type N-linked glycans, but leaving the inner polypeptide-bound carbohydrates, GlcNAc and possibly associated fucose units, intact. Tritium 1-3 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 21-27 9672607-5 1997 Gel filtration of the digested material revealed that [3H]-fucose label was released from gp 120 by this treatment, indicating presence of peripheral fucose units. Tritium 55-57 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 90-96 9672607-6 1997 Furthermore, [3H]-focuse label was also released by treatment of the labelled gp 120 with an alpha-L-fucosidase specifically removing fucose in alpha(1-3) and alpha(1-4) linkages. Tritium 14-16 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 78-84 8794891-10 1996 The rank order of potency of the antagonists in the cell adhesion assays was similar to that of the receptor binding assay, suggesting that the purified alpha v beta 3-[3H]SK&F-107260 binding assay is a valid reflection of the ligand binding to alpha v beta 3 on cell systems. Tritium 169-171 eukaryotic translation elongation factor 1 beta 2 pseudogene 2 Homo sapiens 161-167 8980292-2 1997 Our results indicate that stimulation of HaCaT cells with epidermal growth factor (EGF) or transforming growth factor-alpha (TGF-alpha) within 16 h just prior to reaching confluence amplified the production of calcitriol when calcidiol (3H-25OHD3) was used as a substrate. Tritium 237-239 epidermal growth factor Homo sapiens 58-81 8980292-2 1997 Our results indicate that stimulation of HaCaT cells with epidermal growth factor (EGF) or transforming growth factor-alpha (TGF-alpha) within 16 h just prior to reaching confluence amplified the production of calcitriol when calcidiol (3H-25OHD3) was used as a substrate. Tritium 237-239 epidermal growth factor Homo sapiens 83-86 8980292-3 1997 EGF- and TGF-alpha-induced (0.1-10 nM) 1-hydroxylation of 3H-25OHD3 was concentration-dependent but showed different kinetics. Tritium 58-60 epidermal growth factor Homo sapiens 0-3 8884223-4 1996 High densities of [3H]3-OH-PCP binding sites were found in the stratum radiatum and orients of field CA1 in the hippocampus and in the outer layers of cerebral cortices. Tritium 19-21 carbonic anhydrase 1 Rattus norvegicus 101-104 8761448-11 1996 The addition of heparin or lactoferrin, a known inhibitor of hepatic uptake of chylomicron remnants, to the incubation medium inhibited the uptake of [3H]retinol, present in the lipoprotein core, but not the uptake of the [14C]fatty acids. Tritium 151-153 lactotransferrin Mus musculus 27-38 9048968-1 1997 The ligand binding characteristics of the recombinant human 5-HT1A receptor stably expressed in a Chinese Hamster Ovary (CHO) cell line are described using a selective agonist, [3H]8-OH-DPAT, and a novel antagonist radioligand, [3H]WAY-100635. Tritium 178-180 5-hydroxytryptamine receptor 1A Homo sapiens 60-75 2538309-5 1989 The immunoreactive IGF-I inhibited [125I]IGF-I binding to the IGF-I receptor on chick embryo fibroblasts and stimulated [3H]thymidine incorporation into chick embryo fibroblast DNA. Tritium 121-123 IGF-I Gallus gallus 19-24 9048968-1 1997 The ligand binding characteristics of the recombinant human 5-HT1A receptor stably expressed in a Chinese Hamster Ovary (CHO) cell line are described using a selective agonist, [3H]8-OH-DPAT, and a novel antagonist radioligand, [3H]WAY-100635. Tritium 229-231 5-hydroxytryptamine receptor 1A Homo sapiens 60-75 9048968-6 1997 [3H]WAY-100635 displayed a higher affinity (2-3 fold) for the 5-HT1A site when compared with [3H] 8-OH-DPAT binding under similar incubation conditions. Tritium 1-3 5-hydroxytryptamine receptor 1A Homo sapiens 62-68 9048968-7 1997 Furthermore, [3H] 8-OH-DPAT labelled approximately 53-61% of total 5-HT1A sites recognised by [3H]WAY-100635. Tritium 14-16 5-hydroxytryptamine receptor 1A Homo sapiens 67-73 9048968-7 1997 Furthermore, [3H] 8-OH-DPAT labelled approximately 53-61% of total 5-HT1A sites recognised by [3H]WAY-100635. Tritium 95-97 5-hydroxytryptamine receptor 1A Homo sapiens 67-73 9048968-8 1997 The competition binding profiles of [3H]WAY-100635 and [3H]8-OH-DPAT were highly correlated and consistent with the recognition of 5-HT1A receptors. Tritium 37-39 5-hydroxytryptamine receptor 1A Homo sapiens 131-137 9048968-8 1997 The competition binding profiles of [3H]WAY-100635 and [3H]8-OH-DPAT were highly correlated and consistent with the recognition of 5-HT1A receptors. Tritium 56-58 5-hydroxytryptamine receptor 1A Homo sapiens 131-137 9048968-10 1997 A significant correlation between the respective affinities of a range of agonists and antagonists at recombinant human and rodent hippocampal 5-HT1A binding sites (previously published) was also observed using [3H]WAY-100635 (r = 0.92; P < 0.0005) and [3H]8-OH-DPAT (r = 0.96; P < 0.0005). Tritium 212-214 5-hydroxytryptamine receptor 1A Homo sapiens 143-149 8864541-14 1996 Using 5 nM [3H]-thioperamide, the binding was hardly displaced by H3 agonists within concentration-ranges expected to bind to the histamine H3 receptor. Tritium 11-16 histamine receptor H3 Rattus norvegicus 130-151 8884986-2 1996 LIF stimulation (1 x 10(3) U/ml) for 36 h increased the cell size of neonatal cardiac myocytes and increased [3H] leucine incorporation in both fetal and neonatal cardiac myocytes; the increase was more significant in fetal myocytes. Tritium 110-112 leukemia inhibitory factor Mus musculus 0-3 9048968-10 1997 A significant correlation between the respective affinities of a range of agonists and antagonists at recombinant human and rodent hippocampal 5-HT1A binding sites (previously published) was also observed using [3H]WAY-100635 (r = 0.92; P < 0.0005) and [3H]8-OH-DPAT (r = 0.96; P < 0.0005). Tritium 257-259 5-hydroxytryptamine receptor 1A Homo sapiens 143-149 8832056-15 1996 The incorporation of [3H]-acetate into [3H]-PAF induced by serum-treated zymosan in human PMNs was also inhibited concentration-dependently by cloricromene, with an IC50 of 105 microM. Tritium 22-24 PCNA clamp associated factor Homo sapiens 44-47 9048968-11 1997 The availability of a novel, high-affinity antagonist radioligand, [3H]WAY-100635, will provide a useful tool for the further characterisation of 5-HT1A receptor pharmacology. Tritium 68-70 5-hydroxytryptamine receptor 1A Homo sapiens 146-161 2714781-2 1989 In situ hybridization using an 3H-labelled human G4S genomic DNA fragment to human metaphase chromosomes localized ARSB to chromosome 5q13-5q14. Tritium 31-33 arylsulfatase B Homo sapiens 49-52 9144644-2 1997 Binding studies first established that GR-127,935 shows a 500-fold selectivity for 5-HT1D binding sites (labeled by [3H]L-694,247) versus 5-HT1F binding sites (labeled by [3H]sumatriptan in the presence of 50 nM 5-carboxamidotryptamine) in guinea pig forebrain homogenates (pKD +/- SD = 7.0 +/- 0.2 at 5-HT1F sites and 9.7 +/- 0.1 at 5-HT1D sites). Tritium 117-119 5-hydroxytryptamine receptor 1D Cavia porcellus 83-89 8841942-5 1996 [D-Ala2,D-Leu5]enkephalin (DADLE), a specific delta-agonist, had no inhibition on the binding of [3H]U69593, but displaced the binding of [3H]bremazocine at higher concentrations (10(-5)-10(-6)M). Tritium 139-141 proenkephalin Rattus norvegicus 15-25 8841942-9 1996 Met5-Enkephalin-Arg6-Phe7, a specific kappa 2 ligand, displaced the binding of [3H]bremazocine much more readily than the binding of [3H]U69593. Tritium 80-82 proenkephalin Rattus norvegicus 5-15 2714781-2 1989 In situ hybridization using an 3H-labelled human G4S genomic DNA fragment to human metaphase chromosomes localized ARSB to chromosome 5q13-5q14. Tritium 31-33 arylsulfatase B Homo sapiens 115-119 9306433-4 1997 The ratio between GGH and FPGS activities was better at predicting the amount of polyglutamate accumulated in the 24-h [3H]MTX assay compared to the determination of either activity alone. Tritium 120-122 gamma-glutamyl hydrolase Homo sapiens 18-21 2923910-3 1989 Intravenously administered [3H]retinol bound to retinol binding protein-transthyretin complex (RBP-TTR complex) was used to study the dynamics of circulatory retinol in these rats. Tritium 28-30 retinol binding protein 4 Rattus norvegicus 95-98 9306433-4 1997 The ratio between GGH and FPGS activities was better at predicting the amount of polyglutamate accumulated in the 24-h [3H]MTX assay compared to the determination of either activity alone. Tritium 120-122 folylpolyglutamate synthase Homo sapiens 26-30 9437706-2 1997 On this basis, the aim of the present work was to study the effects of B and C types natriuretic peptides (BNP and CNP) on the uptake, intracellular distribution and release of 3H-NE. Tritium 177-179 2',3'-cyclic nucleotide 3' phosphodiesterase Rattus norvegicus 115-118 8882288-7 1996 Both IGF-I (100 ng ml-1) and IGF-II (100 ng ml-1) and their respective analogues, as well as insulin, all at the same concentration, significantly increased DNA synthesis as determined by [3H]thymidine incorporation (n = 5). Tritium 189-191 insulin Bos taurus 93-100 8661254-5 1996 In contrast, 24 h after training the binding of 3H-AMPA in the CA1 subfield did not differ significantly from naive control values. Tritium 48-50 carbonic anhydrase 1 Rattus norvegicus 63-66 8798241-4 1996 Densities of binding sites for the muscarinic M1 subtype ligand [3H]pirenzepine were reduced by 24-54% (p < 0.01) in frontal cortex, caudate/ putamen, and hippocampal CA1 and CA2 areas. Tritium 65-67 carbonic anhydrase 1 Mus musculus 170-173 8687443-9 1996 In the presence of elf-2B, the exchange of [3H]GDP for GTP from elf-2. Tritium 44-46 ETS-related transcription factor Elf-2 Oryctolagus cuniculus 19-24 8988026-3 1996 Indeed the sigma ligands [3H]haloperidol (Kd = 0.3 nM) and [3H]ifenprodil (Kd = 1.4 nM) bound to a single population of sites in ERG2 wild type yeast microsomes (Bmax values of 77 and 61 pmol/mg of protein, respectively), whereas binding activity was absent in strains carrying ERG2 gene mutations or disruptions. Tritium 26-28 C-8 sterol isomerase ERG2 Saccharomyces cerevisiae S288C 129-133 8988026-3 1996 Indeed the sigma ligands [3H]haloperidol (Kd = 0.3 nM) and [3H]ifenprodil (Kd = 1.4 nM) bound to a single population of sites in ERG2 wild type yeast microsomes (Bmax values of 77 and 61 pmol/mg of protein, respectively), whereas binding activity was absent in strains carrying ERG2 gene mutations or disruptions. Tritium 26-28 C-8 sterol isomerase ERG2 Saccharomyces cerevisiae S288C 278-282 8988026-3 1996 Indeed the sigma ligands [3H]haloperidol (Kd = 0.3 nM) and [3H]ifenprodil (Kd = 1.4 nM) bound to a single population of sites in ERG2 wild type yeast microsomes (Bmax values of 77 and 61 pmol/mg of protein, respectively), whereas binding activity was absent in strains carrying ERG2 gene mutations or disruptions. Tritium 60-62 C-8 sterol isomerase ERG2 Saccharomyces cerevisiae S288C 129-133 8988026-3 1996 Indeed the sigma ligands [3H]haloperidol (Kd = 0.3 nM) and [3H]ifenprodil (Kd = 1.4 nM) bound to a single population of sites in ERG2 wild type yeast microsomes (Bmax values of 77 and 61 pmol/mg of protein, respectively), whereas binding activity was absent in strains carrying ERG2 gene mutations or disruptions. Tritium 60-62 C-8 sterol isomerase ERG2 Saccharomyces cerevisiae S288C 278-282 8988026-6 1996 9E10 c-myc antibodies specifically immunoprecipitated the c-myc tagged protein after [3H]haloperidol photolabeling, unequivocally proving that the drug binding site is localized on the ERG2 gene product. Tritium 86-88 C-8 sterol isomerase ERG2 Saccharomyces cerevisiae S288C 185-189 9117394-9 1996 Finally, the release of [3H]GABA evoked by the combined application of NMDA and carbachol (a treatment known to markedly stimulate arachidonic acid formation) was reduced by inhibitors of phospholipase A2 further indicating that endogenously formed arachidonic acid significantly facilitates the release of GABA in the striatum. Tritium 24-27 phospholipase A2 group IB Rattus norvegicus 188-204 8645989-7 1996 High doses of PAF or phorbol myristate acetate (PMA) downregulated neutrophils and a low dose PAF decreased the specific [3H]PAF binding to eosinophils determined with WEB 2086 at 20 degrees C. Only neutrophils were significantly upregulated by low dose PAF (5 nM), lyso PAF or low dose PMA (1 nM). Tritium 122-124 PCNA clamp associated factor Homo sapiens 14-17 2495009-1 1989 The concentrations of monoamine oxidase-A and -B were determined in homogenates of human cerebral cortex, caudatus and placenta and in human platelet-rich plasma and platelet membranes by determining the specific binding of tritium-labelled pargyline. Tritium 224-231 monoamine oxidase A Homo sapiens 22-48 8645989-7 1996 High doses of PAF or phorbol myristate acetate (PMA) downregulated neutrophils and a low dose PAF decreased the specific [3H]PAF binding to eosinophils determined with WEB 2086 at 20 degrees C. Only neutrophils were significantly upregulated by low dose PAF (5 nM), lyso PAF or low dose PMA (1 nM). Tritium 122-124 PCNA clamp associated factor Homo sapiens 94-97 8645989-7 1996 High doses of PAF or phorbol myristate acetate (PMA) downregulated neutrophils and a low dose PAF decreased the specific [3H]PAF binding to eosinophils determined with WEB 2086 at 20 degrees C. Only neutrophils were significantly upregulated by low dose PAF (5 nM), lyso PAF or low dose PMA (1 nM). Tritium 122-124 PCNA clamp associated factor Homo sapiens 94-97 8645989-7 1996 High doses of PAF or phorbol myristate acetate (PMA) downregulated neutrophils and a low dose PAF decreased the specific [3H]PAF binding to eosinophils determined with WEB 2086 at 20 degrees C. Only neutrophils were significantly upregulated by low dose PAF (5 nM), lyso PAF or low dose PMA (1 nM). Tritium 122-124 PCNA clamp associated factor Homo sapiens 94-97 8645989-7 1996 High doses of PAF or phorbol myristate acetate (PMA) downregulated neutrophils and a low dose PAF decreased the specific [3H]PAF binding to eosinophils determined with WEB 2086 at 20 degrees C. Only neutrophils were significantly upregulated by low dose PAF (5 nM), lyso PAF or low dose PMA (1 nM). Tritium 122-124 PCNA clamp associated factor Homo sapiens 94-97 2720200-5 1989 [3H]Proline incorporation into collagenase digestible protein (CDP) in media was stimulated dose-dependently by IGF-I up to 2.2-fold over the control levels at 130 X 10(-9) M. Addition of 1,25(OH)2D3 (5 X 10(-11) M) and IGF-I further elevated the proline incorporation into CDP. Tritium 1-3 cut-like homeobox 1 Mus musculus 31-61 8769688-4 1996 Human recombinant IGF-I and IGF-II added to cSMC cultured in a medium containing 10% fetal bovine serum (FBS) decreased the cell number and [3H]thymidine incorporation in a dose dependent fashion up to 40% and 43% compared to control cells (100%). Tritium 141-143 insulin like growth factor 2 Homo sapiens 28-34 8953147-2 1996 Native or [3H]methylated fibronectin (FN) or asialofetuin (ASF) were immobilized onto oxidized PET membranes, preactivated or not with carbodiimide (WSC). Tritium 11-13 fibronectin 1 Rattus norvegicus 25-36 2720200-5 1989 [3H]Proline incorporation into collagenase digestible protein (CDP) in media was stimulated dose-dependently by IGF-I up to 2.2-fold over the control levels at 130 X 10(-9) M. Addition of 1,25(OH)2D3 (5 X 10(-11) M) and IGF-I further elevated the proline incorporation into CDP. Tritium 1-3 cut-like homeobox 1 Mus musculus 63-66 2720200-5 1989 [3H]Proline incorporation into collagenase digestible protein (CDP) in media was stimulated dose-dependently by IGF-I up to 2.2-fold over the control levels at 130 X 10(-9) M. Addition of 1,25(OH)2D3 (5 X 10(-11) M) and IGF-I further elevated the proline incorporation into CDP. Tritium 1-3 cut-like homeobox 1 Mus musculus 274-277 8917588-5 1996 [3H]Doxepin has two saturable binding sites with higher and lower affinities in brains of wild-type mice, but H1R-deficient mice showed only the weak labeling of [3H]doxepin that corresponds to lower-affinity binding sites. Tritium 163-165 histamine receptor H1 Mus musculus 110-113 8773462-2 1996 NMDA dose dependently increased [3H]GABA release, which was significantly inhibited by hemoglobin and superoxide scavengers, Cu2+, Zn(2+)-superoxide dismutase and ceruloplasmin. Tritium 33-35 ceruloplasmin Homo sapiens 163-176 2783944-5 1989 This cell population from NZB/W mice secreted IgM and incorporated [3H]TdR at significantly higher levels in response to IL-5, and was more sensitive to IL-5, than a comparable population from several normal murine strains. Tritium 68-70 interleukin 5 Mus musculus 121-125 8621784-2 1996 Inhibition of CDK activity in Adp27KIP1-infected cells was indicated by inhibition of [3H]thymidine incorporation, an increase in cell doubling time and by cell cycle arrest in G1. Tritium 87-89 cyclin dependent kinase inhibitor 1B Homo sapiens 35-39 8904887-6 1996 In 8/10 cases proliferation, measured by 3H-thymidine incorporation, could be induced after CD40 crosslinking, especially in the presence of IL-3. Tritium 41-43 interleukin 3 Homo sapiens 141-145 8980167-7 1996 Estradiol treatment enhanced the LH secretory response while GnRH-induced [3H]arachidonic acid liberation remained unaffected. Tritium 75-78 gonadotropin releasing hormone 1 Rattus norvegicus 61-65 8980167-8 1996 However, progesterone modulated both LH secretion and [3H]arachidonic acid release in response to the GnRH stimulus. Tritium 54-57 gonadotropin releasing hormone 1 Rattus norvegicus 102-106 8910381-4 1996 The GPI1 gene, which encodes a 609-amino acid membrane protein, was cloned by complementation of the temperature sensitivity of gpi1 and corrects the mutant"s [3H]inositol labeling and enzymatic defects. Tritium 160-162 phosphatidylinositol N-acetylglucosaminyltransferase Saccharomyces cerevisiae S288C 4-8 8910381-4 1996 The GPI1 gene, which encodes a 609-amino acid membrane protein, was cloned by complementation of the temperature sensitivity of gpi1 and corrects the mutant"s [3H]inositol labeling and enzymatic defects. Tritium 160-162 phosphatidylinositol N-acetylglucosaminyltransferase Saccharomyces cerevisiae S288C 128-132 8910381-5 1996 Disruption of GPI1 yields viable haploid cells that are temperature-sensitive for growth, for [3H]inositol incorporation into protein, and for GPI anchor-dependent processing of the Gas1/Ggp1 protein and that lack in vitro N-acetylglucosaminylphosphatidylinositol synthetic activity. Tritium 95-97 phosphatidylinositol N-acetylglucosaminyltransferase Saccharomyces cerevisiae S288C 14-18 8863503-7 1996 The cholinesterase inhibitor neostigmine decreased [3H]ACh release at 3 Hz but not at 0.1 Hz; in the presence of atropine, neostigmine potentiated [3H]ACh release, an effect blocked by mecamylamine. Tritium 52-54 butyrylcholinesterase Homo sapiens 4-18 8863503-7 1996 The cholinesterase inhibitor neostigmine decreased [3H]ACh release at 3 Hz but not at 0.1 Hz; in the presence of atropine, neostigmine potentiated [3H]ACh release, an effect blocked by mecamylamine. Tritium 148-150 butyrylcholinesterase Homo sapiens 4-18 8620477-4 1996 Inclusion of IGF-I (10 and 50 ng/ml) in the culture medium resulted in a significant increase of [3H]thymidine incorporation and marked cell proliferation. Tritium 98-100 insulin-like growth factor 1 Rattus norvegicus 13-18 8605977-3 1996 It was also possible to label with [3H]AzHA the isolated uncoupling protein (UcP) of BAT mitochondria with a low stoichiometry--lower than one AzHA per dimeric UcP. Tritium 36-38 uncoupling protein 1 Homo sapiens 77-80 8605977-4 1996 These results together with the observed competition (i.e. prevention of photolabelling) of various UcP anionic substrates with [3H]AzHA and its dodecanoic acid analogue, suggest the existence of the specific fatty acid binding site on UcP identical with the anion channel or anion translocating site. Tritium 129-131 uncoupling protein 1 Homo sapiens 100-103 8605977-4 1996 These results together with the observed competition (i.e. prevention of photolabelling) of various UcP anionic substrates with [3H]AzHA and its dodecanoic acid analogue, suggest the existence of the specific fatty acid binding site on UcP identical with the anion channel or anion translocating site. Tritium 129-131 uncoupling protein 1 Homo sapiens 236-239 8922663-7 1996 5-HT immunopositive neurons and [3H]5-HT uptake were also decreased in ECM cultures. Tritium 33-35 multimerin 1 Homo sapiens 71-74 2519887-2 1989 [3H]Alkylacyl-GPC consisting of molecular species with four or more double bonds (87%) was the major metabolite formed when either [3H]PAF (4 x 10(-6) - 4 x 10(-9) M) or [3H]alkyllyso-GPC (2 x 10(-7) M) was the precursor. Tritium 1-3 glycophorin C Rattus norvegicus 14-17 8922667-7 1996 DNA synthesis measured by [3H]thymidine incorporation was increased to 122% in the presence of serum factors, but inhibited 70% in serum free medium, as a result of TGF beta 2 activity. Tritium 27-29 transforming growth factor, beta 2 Rattus norvegicus 165-175 8786529-2 1996 We studied several histamine homologues as potential ligands for the histamine H3 receptor in two binding assays ([125l]iodophenpropit and N alpha-[3H]methylhistamine binding to rat brain cortex membranes) and two functional H3 receptor models (inhibition of the neurogenic contraction in the guinea pig jejunum and of [3H]noradrenaline release in mouse brain cortex slices). Tritium 148-150 histamine receptor H3 Rattus norvegicus 69-90 8692282-14 1996 The [3H]5-HT binding sites in cerebral cortical synaptosomes and membranes exhibit the pharmacological properties of 5-HT1D receptors. Tritium 5-7 5-hydroxytryptamine receptor 1D Cavia porcellus 117-123 2519887-2 1989 [3H]Alkylacyl-GPC consisting of molecular species with four or more double bonds (87%) was the major metabolite formed when either [3H]PAF (4 x 10(-6) - 4 x 10(-9) M) or [3H]alkyllyso-GPC (2 x 10(-7) M) was the precursor. Tritium 1-3 glycophorin C Rattus norvegicus 184-187 8824628-2 1996 [3H]thymine incorporation experiments indicated that the absence of H-NS inhibited overreplication by the dnaA(Cs) mutant. Tritium 1-3 H-NS Escherichia coli 68-72 2519887-3 1989 However, substantial amounts of [3H]alkyllyso-GPC (mostly in the media) were also generated from [3H]PAF during the early periods of the incubations. Tritium 33-35 glycophorin C Rattus norvegicus 46-49 2519887-3 1989 However, substantial amounts of [3H]alkyllyso-GPC (mostly in the media) were also generated from [3H]PAF during the early periods of the incubations. Tritium 98-100 glycophorin C Rattus norvegicus 46-49 9045087-11 1996 We conclude that [3H]Ro41-1049 and [3H]azabemide are extremely useful radioligands for high-resolution analyses of the abundance and distribution of catalytic sites of monoamine oxidases A and B, respectively, in human brain sections. Tritium 18-20 monoamine oxidase A Homo sapiens 168-194 2519887-5 1989 The [3H]alkylglycerols appear to be produced from [3H]alkyllyso-GPC through the combined actions of lysophospholipase D and a phosphatase. Tritium 5-7 glycophorin C Rattus norvegicus 64-67 8923514-0 1996 Neurotensin, N-acetyl-aspartylglutamate and beta-endorphin modulate [3H]dopamine release from guinea pig nucleus accumbens, prefrontal cortex and caudate-putamen. Tritium 69-71 neurotensin/neuromedin N Cavia porcellus 0-11 8923514-4 1996 We tested neurotensin, N-acetyl-aspartyl-glutamate and beta-endorphin for potential regulation of [3H]dopamine release in these regions of guinea pig brain. Tritium 99-101 neurotensin/neuromedin N Cavia porcellus 10-21 8619229-2 1996 Cd2+ inhibits Na(+)-glucose cotransport in LLC-PK1 cells and in the process decreases the number of binding sites for [3H]phlorizin, a competitive inhibitor of glucose for the Na(+)-glucose cotransporter. Tritium 119-121 CD2 molecule Sus scrofa 0-3 2495224-11 1989 Preincubation of cRCS-X cells with IL1 enhanced their ability to proliferate in response to BCGF or IL5 in [3H]thymidine incorporation assays, but the reverse sequence of cytokine addition showed no effect of IL1. Tritium 108-110 interleukin 1 alpha Homo sapiens 35-38 2918034-6 1989 The increases in incorporation of 3H reflected real increases in proteoglycan synthesis, because chemical analyses showed an increase in the accumulation of macromolecules containing uronic acid in cultures with FGF and TGF-beta or with FGF and IGF-I. Tritium 34-36 insulin-like growth factor I Oryctolagus cuniculus 245-250 8762433-7 1996 It was demonstrated that the antisense c-myc did inhibit the cell growth rate and 3H-TdR incorporation rate of PC-2 cells. Tritium 82-84 MYC proto-oncogene, bHLH transcription factor Homo sapiens 39-44 8772519-7 1996 Nonradiolabeled remnants, human low-density lipoprotein (LDL), anti-LDL receptor antibody, and receptor-associated protein, an LDL-related receptor inhibitor, were all shown to compete for or reduce 3H-remnant uptake. Tritium 199-201 low density lipoprotein receptor Homo sapiens 68-80 8883385-4 1996 Studies using 3H-labeled IAA-phenylalanine showed that the uptake of conjugate from the medium by icr1 was the same as wild type and was reduced by about 25% in icr2. Tritium 14-16 interactor of constitutive active rops 1 Arabidopsis thaliana 98-102 8905167-4 1996 Circulating [3H]PAM is incorporated into sn-1 positions of brain phospholipids, mainly phosphatidylcholine, and its rate of turnover is thought to reflect turnover of neuronal and glial membranes. Tritium 13-15 peptidylglycine alpha-amidating monooxygenase Rattus norvegicus 16-19 8905167-5 1996 These results, when related to published evidence of altered cortical phospholipid metabolism in NBM-lesioned rats, suggest that images of increased [3H]PAM incorporation into ipsilateral cortex reflect increased membrane remodeling involving phospholipids. Tritium 150-152 peptidylglycine alpha-amidating monooxygenase Rattus norvegicus 153-156 8897640-4 1996 Results showed that BNP (100 nM) and CNP (1, 10 and 100 nM) enhanced total and neuronal [3H]NE uptake but did not modify non-neuronal uptake. Tritium 89-91 2',3'-cyclic nucleotide 3' phosphodiesterase Rattus norvegicus 37-40 8703030-11 1996 Strikingly, the concurrent addition of IL-4 enhanced PDGF BB-induced Iepsilon binding activity, Jak1 tyrosine phosphorylation, and [3H]thymidine incorporation. Tritium 132-134 interleukin 4 Mus musculus 39-43 8853405-2 1996 This study defined the nature of gastrin binding in human colon cancer using [3H]L-365,260, a specific cholecystokinin B (CCK-B)/gastrin antagonist found to block gastrin"s effects on growth. Tritium 78-80 gastrin Homo sapiens 33-40 8818201-6 1996 IGF-I and IGF-II (but to a lesser extent) stimulation was due to an increased DNA synthesis (3H-thymidine uptake) as well as protein anabolism (incorporated proline). Tritium 93-95 insulin-like growth factor 1 Rattus norvegicus 0-5 8688833-4 1996 Porcine insulin and human epidermal growth factor (EGF) stimulated [3H]-leucine and [3H]-thymidine incorporation in hemocytes in a dose-dependent manner. Tritium 68-70 epidermal growth factor Homo sapiens 26-49 8688833-4 1996 Porcine insulin and human epidermal growth factor (EGF) stimulated [3H]-leucine and [3H]-thymidine incorporation in hemocytes in a dose-dependent manner. Tritium 68-70 epidermal growth factor Homo sapiens 51-54 8688833-4 1996 Porcine insulin and human epidermal growth factor (EGF) stimulated [3H]-leucine and [3H]-thymidine incorporation in hemocytes in a dose-dependent manner. Tritium 85-87 epidermal growth factor Homo sapiens 26-49 8688833-4 1996 Porcine insulin and human epidermal growth factor (EGF) stimulated [3H]-leucine and [3H]-thymidine incorporation in hemocytes in a dose-dependent manner. Tritium 85-87 epidermal growth factor Homo sapiens 51-54 8906117-2 1996 Specific binding of [3H]WEB 2086, a specific ligand for PAF receptor, to peripheral blood eosinophils obtained from healthy individuals was significantly increased when the cells were cultured with IL-3, IL-5, or GM-CSF for 12 h, compared with untreated cells. Tritium 21-23 PCNA clamp associated factor Homo sapiens 56-59 8906117-2 1996 Specific binding of [3H]WEB 2086, a specific ligand for PAF receptor, to peripheral blood eosinophils obtained from healthy individuals was significantly increased when the cells were cultured with IL-3, IL-5, or GM-CSF for 12 h, compared with untreated cells. Tritium 21-23 interleukin 3 Homo sapiens 198-202 8906117-2 1996 Specific binding of [3H]WEB 2086, a specific ligand for PAF receptor, to peripheral blood eosinophils obtained from healthy individuals was significantly increased when the cells were cultured with IL-3, IL-5, or GM-CSF for 12 h, compared with untreated cells. Tritium 21-23 colony stimulating factor 2 Homo sapiens 213-219 2540993-0 1989 Chronic administration of morphine and naltrexone up-regulate mu-opioid binding sites labeled by [3H][D-Ala2,MePhe4,Gly-ol5]enkephalin: further evidence for two mu-binding sites. Tritium 98-100 proenkephalin Rattus norvegicus 124-134 9320352-8 1996 The results provide evidence for at least three different high-affinity [3H]5-HT binding sites, one of them showing a pharmacological profile strikingly similar to that of the mammalian 5-HT1A receptor. Tritium 73-75 5-hydroxytryptamine receptor 1A Homo sapiens 186-201 8695660-7 1996 The effect of expressing either form of SCP-2 on [3H]cholesterol uptake was determined. Tritium 50-52 sterol carrier protein 2, liver Mus musculus 40-45 8695660-9 1996 The [3H]cholesterol uptake rate in 15 kDa SCP-2 expressing cells was increased 1.3-fold, while the extent of [3H]cholesterol uptake was increased 1.4-fold after 12 h of uptake compared to control L-cells. Tritium 5-7 sterol carrier protein 2, liver Mus musculus 42-47 8649777-3 1996 Treatment of cells with 25 pM EGF produced an increase in [3H]thymidine incorporation in A431, HN6 and HN30 cells, with respect to control cultures. Tritium 59-61 epidermal growth factor Homo sapiens 30-33 8649777-4 1996 Exposure to 2.5 nM EGF reduced [3H]thymidine incorporation in A431 cells and HN6 cells to 11% and 70% of control levels, respectively, whereas HN30 cells continued to proliferate in the presence of EGF. Tritium 32-34 epidermal growth factor Homo sapiens 19-22 2540993-5 1989 A prediction based upon these observations is that, using [3H][D-Ala2,MePhe4,Gly-ol5]enkephalin to label mu binding sites, chronic morphine should upregulate only the mu cx binding site, whereas chronic naltrexone should additionally up-regulate the mu ncx binding site. Tritium 59-61 proenkephalin Rattus norvegicus 85-95 8843957-7 1996 The opposite effect was observed on the level of the 3H-labeled (ALAS-N) mRNA. Tritium 53-55 aminolevulinic acid synthase 1 Mus musculus 65-71 2539233-2 1989 A saturating concentration of the mu-specific ligand [3H]D-ala2-N-methyl-Phe4,Gly-ol5-enkephalin was used to label sites in slide-mounted sections through one level of the thalamus in rats implanted subcutaneously with morphine pellets for 5 days. Tritium 54-56 proenkephalin Rattus norvegicus 86-96 8868030-7 1996 Addition of synthetic retinoids with a terminal carboxyl (CD270, CD271, CD367 and Ro 13-7410) decreased the [3H]ddROH accumulation with about 70%, but hardly at all affected the accumulation of [3H]ROH. Tritium 109-111 nerve growth factor receptor Homo sapiens 65-70 8719789-4 1995 GR203040 potently inhibited [3H]-substance P binding to human NK1 receptors expressed in Chinese hamster ovary (CHO) and U373 MG astrocytoma cells, and NK1 receptors in ferret and gerbil cortex (pKi values of 10.3, 10.5, 10.1 and 10.1 respectively). Tritium 29-31 tachykinin receptor 1 Homo sapiens 62-65 9162203-0 1996 Autoradiography with [3H]8-OH-DPAT reveals increases in 5-HT(1A) receptors in ventral prefrontal cortex in schizophrenia. Tritium 22-24 5-hydroxytryptamine receptor 1A Homo sapiens 56-63 9162203-2 1996 In view of the evidence that one serotonin (5-HT) receptor, the 5-HT(1A) subtype, is associated with cortical glutamatergic neurons, we have used quantitative receptor autoradiography to measure the specific binding of the 5-HT(1A) receptor ligand [3H]8-OH-DPAT (2 nM) in sections of orbital frontal cortex taken from 18 control and 12 schizophrenic postmortem brains. Tritium 249-251 5-hydroxytryptamine receptor 1A Homo sapiens 64-71 9162203-2 1996 In view of the evidence that one serotonin (5-HT) receptor, the 5-HT(1A) subtype, is associated with cortical glutamatergic neurons, we have used quantitative receptor autoradiography to measure the specific binding of the 5-HT(1A) receptor ligand [3H]8-OH-DPAT (2 nM) in sections of orbital frontal cortex taken from 18 control and 12 schizophrenic postmortem brains. Tritium 249-251 5-hydroxytryptamine receptor 1A Homo sapiens 223-240 2730238-2 1989 [3H]-QNB accumulated in the hippocampus of 3 month old rats, primarily in the stratum oriens of the CA1 and CA2 fields, followed in descending order by the molecular layer of granule cells of the dentate gyrus and the CA3 field. Tritium 1-3 carbonic anhydrase 1 Rattus norvegicus 100-103 2730238-4 1989 In contrast, the density of [3H]-QNB binding sites in the hippocampus of 22 months old rats was significantly reduced, mainly in the granule cells of the dentate gyrus, next in the CA3 and in the CA1-CA2 fields. Tritium 29-31 carbonic anhydrase 1 Rattus norvegicus 196-199 8632144-4 1996 The glutamate site antagonist D, L-(epsilon)-2-[3H]amino-4-propyl-5-phosphono-3-pentanoic acid ([3H]CGP 39653) had high affinity for NR1a/NR2A receptors (KD = 3.93 nM) but did not bind to NR1a/NR2B receptors. Tritium 48-50 glutamate receptor, ionotropic, NMDA2A (epsilon 1) Mus musculus 138-142 8632144-4 1996 The glutamate site antagonist D, L-(epsilon)-2-[3H]amino-4-propyl-5-phosphono-3-pentanoic acid ([3H]CGP 39653) had high affinity for NR1a/NR2A receptors (KD = 3.93 nM) but did not bind to NR1a/NR2B receptors. Tritium 48-50 glutamate receptor, ionotropic, NMDA2B (epsilon 2) Mus musculus 193-197 7500171-4 1995 Following intraportal injection of a flooding dose of [3H]phenylalanine, in vivo hepatic apo A-I synthesis and secretion were significantly greater in the copper-deficient animals as detected by [3H]phenylalanine incorporation into immunoprecipitable apo A-I isolated from liver homogenates and plasma using anti-rat apo A-I antibodies. Tritium 55-57 apolipoprotein A1 Rattus norvegicus 89-96 7500171-4 1995 Following intraportal injection of a flooding dose of [3H]phenylalanine, in vivo hepatic apo A-I synthesis and secretion were significantly greater in the copper-deficient animals as detected by [3H]phenylalanine incorporation into immunoprecipitable apo A-I isolated from liver homogenates and plasma using anti-rat apo A-I antibodies. Tritium 55-57 apolipoprotein A1 Rattus norvegicus 251-258 8531137-8 1995 The cloned rabbit B2 receptor expressed in COS-1 cells binds [3H]BK in a saturable manner (KD 2.1 nM) and this ligand competes with a series of kinin agonists and antagonist with a rank order consistent with the B2 receptor identity. Tritium 62-64 bradykinin receptor B2 Homo sapiens 18-29 8804693-5 1996 The decrease in KCI-induced changes in AMPA receptor binding in young animals was also associated with an altered capacity of the bee venom peptide, mellitin (a phospholipase A2 (PLA2) activator), to increase [3H]AMPA binding in synaptoneurosomes. Tritium 210-212 phospholipase A2 group IB Rattus norvegicus 161-177 2730238-6 1989 ), started at 16 months of age and continued for 6 months, caused a dose-related restoration of the number of [3H]-QNB binding sites, primarily in the CA1 and CA2 fields and in lesser amounts of the dentate gyrus. Tritium 111-113 carbonic anhydrase 1 Rattus norvegicus 151-154 2537620-5 1989 At concentrations of the amiloride analogues which block Na+/H+ exchange in both cell types by 76-98%, the EGF-dependent alterations in [3H]thymidine incorporation or induction in c-myc or c-fos gene transcription were unaltered. Tritium 137-139 epidermal growth factor Homo sapiens 107-110 8639615-5 1996 The results showed that hepatic uptake of the cholesteryl esters analog [3H]cholesteryl ether on reconstituted HDL was inhibited by anti-cholesterol esterase antibodies. Tritium 73-76 carboxyl ester lipase Homo sapiens 137-157 8524258-3 1995 Both Cys-3 and Cys-5 were apparent sites of palmitoylation in Lck expressed in fibroblasts, as only the simultaneous mutation of both Cys-3 and Cys-5 caused a large reduction in the incorporation of [3H]palmitic acid. Tritium 200-202 LCK proto-oncogene, Src family tyrosine kinase Homo sapiens 62-65 8550082-3 1995 Our data clearly show that MCP-1 (200 nM) causes a marked release of [3H]serotonin ([3H]5HT and histamine, which reach a peak at 40 min of incubation (56.6 +/- 5.3 and 34.7 +/- 6 above the control, respectively). Tritium 70-72 C-C motif chemokine ligand 2 Rattus norvegicus 27-32 8550082-3 1995 Our data clearly show that MCP-1 (200 nM) causes a marked release of [3H]serotonin ([3H]5HT and histamine, which reach a peak at 40 min of incubation (56.6 +/- 5.3 and 34.7 +/- 6 above the control, respectively). Tritium 85-87 C-C motif chemokine ligand 2 Rattus norvegicus 27-32 8550082-4 1995 In dose-response experiments, MCP-1 (200, 100, 50, 25, 12.5, 6.25 and 3.12 nM) provoked a dose-dependent release of [3H]5HT and histamine from RPMC, which was maximum at 200 nM. Tritium 117-119 C-C motif chemokine ligand 2 Rattus norvegicus 30-35 8724036-10 1996 Finally, gp120 reversed (1 nM) and surmounted (10nM) the antagonism by 10 microM 7-chlorokynurenate of the NMDA-evoked [3H]-noradrenaline release. Tritium 120-123 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 9-14 2708007-4 1989 3H-heparin binding to peptide F-9 was specific as determined by competition with excess unlabeled heparin, dextran sulfate, and dermatan sulfate. Tritium 0-2 coagulation factor IX Mus musculus 30-33 8624249-3 1996 CYP1A1 mRNA was detected by in situ hybridization with a 3H-labeled RNA probe and quantitated by image analysis, whereas CYP1A1 protein was detected by immunohistochemical staining with an avidin-biotin complex procedure. Tritium 57-59 cytochrome P450, family 1, subfamily a, polypeptide 1 Mus musculus 0-6 8708541-11 1996 In studies on IGFBP-6 actions, IGFBP-6 completely inhibited IGF-II-induced [3H]thymidine incorporation in MC3T3-E1 mouse osteoblast cells while it had only minimal inhibitory effects on IGF-I-induced [3H]thymidine incorporation. Tritium 76-78 insulin-like growth factor 2 Mus musculus 60-66 8566181-3 1995 In addition, it was demonstrated that not only receptors consisting of alpha 1 beta 3 gamma 3, but also those consisting of alpha 1 gamma 2 subunits exhibited [3H]flunitrazepam binding which could be stimulated by GABA. Tritium 160-162 adrenoceptor alpha 1D Homo sapiens 71-93 8566181-3 1995 In addition, it was demonstrated that not only receptors consisting of alpha 1 beta 3 gamma 3, but also those consisting of alpha 1 gamma 2 subunits exhibited [3H]flunitrazepam binding which could be stimulated by GABA. Tritium 160-162 adrenoceptor alpha 1D Homo sapiens 71-78 7568228-8 1995 Exogenous chicken IGF-I, human insulin, and human proinsulin added to E6 cultured neuroretinas showed relatively close potencies stimulating proliferation, as determined by [methyl-3H]thymidine incorporation, with a plateau reached at 10(-8) M. These factors also stimulated neuronal differentiation, indicated by the expression of the neuron-specific antigen G4. Tritium 181-183 insulin like growth factor 1 Gallus gallus 18-23 8708541-11 1996 In studies on IGFBP-6 actions, IGFBP-6 completely inhibited IGF-II-induced [3H]thymidine incorporation in MC3T3-E1 mouse osteoblast cells while it had only minimal inhibitory effects on IGF-I-induced [3H]thymidine incorporation. Tritium 201-203 insulin-like growth factor 2 Mus musculus 60-66 2709436-0 1989 Cutaneous absorption and decontamination of [3H]T-2 toxin in the rat model. Tritium 45-47 brachyury 2 Rattus norvegicus 48-51 8621590-10 1996 Overexpression of Crk also enhanced IGF-I-induced mitogenesis of NIH-3T3 cells, as measured by [3H]thymidine incorporation. Tritium 96-98 v-crk avian sarcoma virus CT10 oncogene homolog Mus musculus 18-21 8612674-5 1996 [3H]Thymidine incorporation was inhibited by rTIMP-2 treatment in the nonadherent cell line HL60. Tritium 1-3 TIMP metallopeptidase inhibitor 2 Rattus norvegicus 45-52 7561874-6 1995 To determine which segments of the tau protein are susceptible to modification by transglutaminase, tau was labeled with [3H]putrescine by transglutaminase and proteolyzed with alpha-chymotrypsin, and the breakdown products were analyzed. Tritium 122-124 microtubule associated protein tau Homo sapiens 100-103 2709436-4 1989 Less than 1% of the applied dose was absorbed in 5 min, and 50% was absorbed in 24 h. At 5 min, 99.5 +/- 0.05% of nonabsorbed (residual) [3H]T-2 was removed, and 58 +/- 5.2% of residual toxin was removed at 24 h with a 2.5% detergent/water spray. Tritium 138-140 brachyury 2 Rattus norvegicus 141-144 7650054-5 1995 By contrast, treatment of T51B cells with EGF results in a slower, more prolonged extracellular Ca(2+)-dependent increase in both [3H]-glycerol radiolabeled diacyl-glycerol, and diacylglycerol mass, an increase in choline release into the extracellular medium, and eventually a substantial DNA synthesis. Tritium 131-133 epidermal growth factor Homo sapiens 42-45 7562471-3 1995 Except for the 0.5 dose molar ratio, the kinetics of [3H]colchicine efflux from lymphocytes induced by extracellular specific Fab fragments were similar to those obtained by washing and were characterized by a first-order decline with half-lives ranging from 15.5 to 16.4 hr. Tritium 54-56 FA complementation group B Homo sapiens 126-129 7562532-2 1995 This study has used [3H]-[D-Ala2,MePhe4,Gly- (ol)5(2)]-enkephalin, a highly selective ligand, to label mu-opioid receptors in both membranes and tissue sections. Tritium 21-23 proenkephalin Rattus norvegicus 55-65 8584041-0 1995 [3H]sumatriptan labels both 5-HT1D and 5-HT1F receptor binding sites in the guinea pig brain: an autoradiographic study. Tritium 1-3 5-hydroxytryptamine receptor 1D Cavia porcellus 28-34 8584041-0 1995 [3H]sumatriptan labels both 5-HT1D and 5-HT1F receptor binding sites in the guinea pig brain: an autoradiographic study. Tritium 1-3 5-hydroxytryptamine receptor 1F Rattus norvegicus 39-45 8726354-3 1996 Both alpha 2M-methylamine and a cloned and expressed 20-kDa receptor binding fragment (RBF) from rat alpha 1M+, stimulated macrophage synthesis of PAF from [3H]acetate, [3H]methylcholine, and 1-O-[3H]alkyl lyso-PAF by two- to threefold. Tritium 157-159 PCNA clamp associated factor Homo sapiens 147-150 8793911-2 1996 The selective 5-HT1A receptor agonist 8-hydroxy-2-(di-n-propylamino)-tetralin (8-OH-DPAT) and 5-hydroxytryptamine (5-HT) caused concentration-dependent inhibitions of the electrically evoked release of [3H]acetylcholine from myenteric plexus preparations that had been preincubated with [3H]choline. Tritium 203-205 5-hydroxytryptamine receptor 1A Cavia porcellus 14-20 8602176-3 1996 T lymphocytes from spleen were cloned in vitro and HPRT deficient clones were detected by double-labeling with [3H]thymidine and [14C]hypoxanthine. Tritium 112-114 hypoxanthine guanine phosphoribosyl transferase Mus musculus 51-55 8584041-13 1995 [3H]5-HT labeled 5-HT1F sites in the claustrum and intermediate cortical layers in the guinea-pig. Tritium 1-3 5-hydroxytryptamine receptor 1F Rattus norvegicus 17-23 2552241-1 1989 Binding characteristics of a new, conformationally constrained, halogenated enkephalin analogue, [3H]-[D-penicillamine2, pCl-Phe4, D-penicillamine5]enkephalin ([3H]pCl-DPDPE), were determined using homogenized rat brain tissue. Tritium 98-100 proenkephalin Rattus norvegicus 76-86 8538858-2 1995 Membranes from GT1 cells exhibited high-affinity binding for the specific alpha 2-AR radioligand [3H]RX821002 (Kd = 0.2 +/- 0.03 nM, Bmax = 29.5 +/- 2.1 fmol/mg protein, n = 3). Tritium 98-100 beta-1,4-galactosyltransferase 1 Homo sapiens 15-18 8640815-7 1996 Another metabolite of 4-OH-tam, assumed to be 3,4-dihydroxytamoxifen (3,4-di-OH-tam) catechol, was demonstrated by its monomethylation with [3H]S-adenosyl-L-methionine ([3H]SAM) in presence of endogenous catechol-O-methyltransferase. Tritium 141-143 catechol-O-methyltransferase Rattus norvegicus 204-232 8640815-7 1996 Another metabolite of 4-OH-tam, assumed to be 3,4-dihydroxytamoxifen (3,4-di-OH-tam) catechol, was demonstrated by its monomethylation with [3H]S-adenosyl-L-methionine ([3H]SAM) in presence of endogenous catechol-O-methyltransferase. Tritium 141-144 catechol-O-methyltransferase Rattus norvegicus 204-232 8724600-3 1996 For MP1, the displacement of [3H]DAGO (selective mu-agonist) by IC50 = 7.3 x 10(-5) M and [3H]DSLET (selective delta-agonist) with IC50 = 7.0 x 10(-5) M. The data obtained suggest that the hypoalgesic effect of the peptides cannot be due to their direct interaction with opioid receptors. Tritium 30-32 pitrilysin metallepetidase 1 Mus musculus 4-7 2552241-1 1989 Binding characteristics of a new, conformationally constrained, halogenated enkephalin analogue, [3H]-[D-penicillamine2, pCl-Phe4, D-penicillamine5]enkephalin ([3H]pCl-DPDPE), were determined using homogenized rat brain tissue. Tritium 98-100 proenkephalin Rattus norvegicus 148-158 7542171-8 1995 Finally, the proliferation of the cultured cancer cells was stimulated by exogenous G-CSF administration, and this stimulation was inhibited by adding anti-G-CSF antibody, as demonstrated by both the flow cytometric bromodeoxyuridine incorporation technique and the [3H]thymidine incorporation assay. Tritium 267-269 colony stimulating factor 3 Homo sapiens 84-89 2552241-1 1989 Binding characteristics of a new, conformationally constrained, halogenated enkephalin analogue, [3H]-[D-penicillamine2, pCl-Phe4, D-penicillamine5]enkephalin ([3H]pCl-DPDPE), were determined using homogenized rat brain tissue. Tritium 161-163 proenkephalin Rattus norvegicus 148-158 7542171-8 1995 Finally, the proliferation of the cultured cancer cells was stimulated by exogenous G-CSF administration, and this stimulation was inhibited by adding anti-G-CSF antibody, as demonstrated by both the flow cytometric bromodeoxyuridine incorporation technique and the [3H]thymidine incorporation assay. Tritium 267-269 colony stimulating factor 3 Homo sapiens 156-161 2525822-4 1989 In competition experiments 8-OH-DPAT, TFMPP, mesulergine, DOB, and ICS 205-930 had low affinity for 3H-5HT-labeled 5HT1D sites, indicating that the pharmacology of the 5HT1D site is distinct from previously identified 5HT1A, 5HT1B, 5HT1C, 5HT2, and 5HT3 sites. Tritium 100-102 5-hydroxytryptamine receptor 1A Bos taurus 218-223 7556543-7 1995 In grafts from [3H]thymidine-labeled donors, an appreciable number of CV in E14 grafts were identified as intrinsic to the muscle. Tritium 16-18 nuclear protein, coactivator of histone transcription Homo sapiens 76-79 8603600-10 1996 Coadministration of 30 micrograms IGF-2 blocked the neuroprotective effects of 20 micrograms IGF-1 (n = 18, P < 0.05) and reduced the accumulation of [3H]IGF-1 in the injured hemisphere (n = 4) (P < 0.05). Tritium 154-156 insulin-like growth factor 1 Rattus norvegicus 157-162 2629172-0 1989 Hepatic subcellular distribution of [3H]T-2 toxin. Tritium 37-39 brachyury 2 Rattus norvegicus 40-43 8619888-9 1996 The binding of AhRBFs reconstituted to dsDNA was shown, by competition experiments with Ah receptor bound by unlabeled TCDD (TCDD-R), to be > 90% specific for [3H]TCDD-R. Tritium 163-165 aryl hydrocarbon receptor Oryctolagus cuniculus 88-99 8593828-8 1996 However, chronic stimulation of [3H]-NE uptake involves PKC, cfos, and NET gene transcription. Tritium 33-35 solute carrier family 6 member 2 Rattus norvegicus 71-74 7635810-4 1995 Analysis of the VirG amino acid sequence suggested that virG encodes a lipoprotein, which was confirmed by [3H]palmitate labeling of VirG-PhoA fusion proteins. Tritium 108-111 required for localization of YscC Yersinia enterocolitica 16-20 7635810-4 1995 Analysis of the VirG amino acid sequence suggested that virG encodes a lipoprotein, which was confirmed by [3H]palmitate labeling of VirG-PhoA fusion proteins. Tritium 108-111 required for localization of YscC Yersinia enterocolitica 56-60 2629172-1 1989 The subcellular distribution of T-2 mycotoxin and its metabolites was studied in isolated rat livers perfused with [3H]T-2 toxin. Tritium 116-118 brachyury 2 Rattus norvegicus 32-35 8789288-10 1995 In contrast to IL-10, IL-4 also inhibited the spontaneous (3/5) and cytokine induced (5/5) secretion of G-CSF and GM-CSF (4/5) protein in the cases in which an enhancement of the 3H-TdR uptake was noticed. Tritium 179-181 colony stimulating factor 3 Homo sapiens 104-109 8732447-4 1996 When granulosa cells were incubated with IGF-I in the presence of various concentrations of follicle-stimulating hormone (FSH), DNA synthesis as assessed by [3H]thymidine incorporation was increased only in the presence of low doses of FSH. Tritium 158-160 insulin-like growth factor 1 Rattus norvegicus 41-46 2629172-1 1989 The subcellular distribution of T-2 mycotoxin and its metabolites was studied in isolated rat livers perfused with [3H]T-2 toxin. Tritium 116-118 brachyury 2 Rattus norvegicus 119-122 8591854-4 1996 Activin A, at a concentration of 10(-9) mol/L, blocked the effect of epidermal growth factor (EGF) on DNA synthesis, that was assessed by measuring [3H] thymidine incorporation and nuclear labeling, almost completely, and NE reversed the inhibitory effect of activin A on DNA synthesis. Tritium 149-151 inhibin subunit beta A Rattus norvegicus 0-9 8632339-8 1996 5-HT1D receptors, similarly to 5-HT1A autoreceptors, desensitize after long-term treatment with a selective 5-HT reuptake inhibitor or a reversible type A monoamine oxidase inhibitor because the efficacy of sumatriptan and of 8-OH-DPAT to inhibit the electrically evoked overflow of [3H]5-HT was reduced after the administration of either drug. Tritium 284-286 5-hydroxytryptamine receptor 1D Rattus norvegicus 0-6 8730846-3 1996 The present study determined the distribution of [3H]IGF-I given into the lateral ventricle after unilateral HI injury in adult rats. Tritium 50-52 insulin-like growth factor 1 Rattus norvegicus 53-58 7672038-4 1995 [3H]Nimodipine binding to the nonischemic hemisphere was characterized by one binding site with regional binding affinity (KD) estimates ranging from 221 to 482 pM and maximal binding site densities (BMAX) ranging from 13.2 (9.6-17.5) pmol/g tissue (estimate and 95% confidence interval) in CA1 to 32.5 (26.5-39.9) pmol/g tissue in dentate. Tritium 1-3 carbonic anhydrase 1 Rattus norvegicus 291-294 7672038-5 1995 [3H]CGS-19755 binding to the nonischemic hemisphere was characterized by KD estimates ranging from 59 to 97 nM and BMAX values ranging from 143 (108-192) pmol/g tissue in cortex to 569 (515-641) pmol/g tissue in CA1. Tritium 1-3 carbonic anhydrase 1 Rattus norvegicus 212-215 7564919-4 1995 We find that both GM-CSF and SLF induced increased phosphatidylcholine (PC) turnover rates (biosynthesis and degradation) as measured by increased [3H]-choline labelling, with SLF being more potent than GM-CSF after 6 h of stimulation, but equipotent at 24 h of stimulation. Tritium 148-150 colony stimulating factor 2 Homo sapiens 18-24 7541943-1 1995 Recombinant human insulin-like growth factor binding protein-3 (rhIGFBP-3) effects on basal, insulin-like growth factor I (IGF-I)-, and follicle-stimulating hormone (FSH)-stimulated progesterone (P4) secretion and [3H]aminoisobutyric acid (AIB) uptake by primary porcine granulosa cells (MDGs) and MDGs that have been passaged once (MDGp1) were assessed. Tritium 215-217 insulin like growth factor binding protein 3 Homo sapiens 18-62 7541943-5 1995 However, the IGF-I-stimulated increase in [3H]AIB uptake was completely prevented by concurrent treatment with IGFBP-3. Tritium 43-45 insulin like growth factor binding protein 3 Homo sapiens 111-118 8695163-2 1996 Tumor tissue TS levels in fresh frozen surgical biopsies from 136 patients with gastrointestinal or breast cancer, not previously subjected to chemotherapy, were analysed by [3H]FdUMP radioligand binding assay. Tritium 175-177 thymidylate synthetase Homo sapiens 13-15 8788510-7 1996 Another possibility would be that [3H]5-CT labels 5-HT1A receptors in the low-affinity state. Tritium 35-37 5-hydroxytryptamine receptor 1A Homo sapiens 50-56 2629172-11 1989 The time course for distribution of radiolabel in liver suggested an immediate association of [3H]T-2 with plasma membranes and a subsequent association of toxin and metabolites with endoplasmic reticulum, mitochondria and nuclei, the known sites of action of this toxin. Tritium 95-97 brachyury 2 Rattus norvegicus 98-101 3058209-6 1988 The result of the incubation of [3H]intrinsic factor or [3H]haptocorrin with chymotrypsin and trypsin gave, respectively, no change in the elution position and a shift corresponding to a decrease of 50% of the estimated molecular mass. Tritium 33-35 cobalamin binding intrinsic factor Homo sapiens 36-52 8804551-5 1996 In both AL- and DR-mice total BaP-DNA adduct formation in lung reached a peak at 48 hours after treatment with [3H]BaP and the in vivo formation of BaP-N2-dG was greater in DR mouse lung than in that of AL-animals by 22%. Tritium 112-114 prohibitin 2 Mus musculus 30-33 8804551-5 1996 In both AL- and DR-mice total BaP-DNA adduct formation in lung reached a peak at 48 hours after treatment with [3H]BaP and the in vivo formation of BaP-N2-dG was greater in DR mouse lung than in that of AL-animals by 22%. Tritium 112-114 prohibitin 2 Mus musculus 115-118 8804551-5 1996 In both AL- and DR-mice total BaP-DNA adduct formation in lung reached a peak at 48 hours after treatment with [3H]BaP and the in vivo formation of BaP-N2-dG was greater in DR mouse lung than in that of AL-animals by 22%. Tritium 112-114 prohibitin 2 Mus musculus 115-118 7554520-5 1995 IGF-1 caused a 2.4-fold increase in mesangial cell proliferation as measured by 3H-thymidine incorporation. Tritium 80-82 insulin-like growth factor 1 Rattus norvegicus 0-5 7758156-9 1995 The antibody prevented the myocardial protein synthesis induced by myotrophin as revealed by the blockage of the stimulation of [3H]leucine incorporation into myocyte protein. Tritium 129-131 myotrophin Rattus norvegicus 67-77 3058209-6 1988 The result of the incubation of [3H]intrinsic factor or [3H]haptocorrin with chymotrypsin and trypsin gave, respectively, no change in the elution position and a shift corresponding to a decrease of 50% of the estimated molecular mass. Tritium 57-59 transcobalamin 1 Homo sapiens 60-71 7760041-6 1995 The observed changes in muscimol-stimulated 36CI- uptake at the FK stage closely parallel the recently observed changes in [3H]muscimol binding in the CA1 area and fascia dentata. Tritium 124-126 carbonic anhydrase 1 Rattus norvegicus 151-154 8576277-2 1996 The initial cellular uptake of [3H]ZD1694 was greater in K562 cells than in MOLT-3 cells and the drug accumulated approximately 3-fold more in the former cells following incubation with 0.1 microM ZD1694 at 37 degrees C for 24 h. TS and dihydrofolate reductase activities were not significantly different between the two cell lines. Tritium 32-34 thymidylate synthetase Homo sapiens 230-232 2462362-8 1988 NKA, NKB, and SP caused a dose-dependent, tetrodotoxin-sensitive increase in [3H]acetylcholine release from strips preincubated with [3H]choline. Tritium 78-80 tachykinin precursor 3 Homo sapiens 5-8 7720660-6 1995 Estrogen enhanced the [3H]-thymidine incorporation into the cell in dose- and time-dependent manners in culture: estrogen treatment for more than 12 h significantly (P < 0.05) enhanced the [3H]-thymidine incorporation into the cell at 10(-8) M. The estrogen-induced cell growth was observed in association with the increase in EGF, TGF alpha, and EGF receptor messenger RNA levels by estrogen. Tritium 193-195 epidermal growth factor Homo sapiens 330-333 7720660-9 1995 Anti-EGF antibody significantly reduced the estrogen-induced increase in [3H]-thymidine incorporation, whereas anti-TGF alpha antibody failed to show the effect. Tritium 74-76 epidermal growth factor Homo sapiens 5-8 8645616-9 1996 These analyses demonstrated that the [3H]aldosterone-MBP-HBD complex is at least associated with hsp90 in reticulocyte lysate and that the HBD of hMR is sufficient to bind hsp90. Tritium 38-40 heat shock protein 90 alpha family class A member 1 Homo sapiens 97-102 2462362-8 1988 NKA, NKB, and SP caused a dose-dependent, tetrodotoxin-sensitive increase in [3H]acetylcholine release from strips preincubated with [3H]choline. Tritium 134-136 tachykinin precursor 3 Homo sapiens 5-8 7642170-2 1995 3H-thymidine incorporation in islets increased with increasing glucose up to a concentration of 21.1 mM in both free-floating islets (11,789 cpm/micrograms DNA +/- 1,610 SEM) and islets attached to fibronectin coated plastic (43,043 cpm/micrograms DNA +/- 9,203 SEM). Tritium 0-2 fibronectin 1 Rattus norvegicus 198-209 3058540-8 1988 Under the influence of BMP/NCP, cultures grew on deactivated matrix, incorporated 55% more [3H]thymidine into DNA, incorporated 115% more [35S]sulfate into GAG than control cultures, and differentiated into cartilage. Tritium 92-94 bone morphogenetic protein 1 Homo sapiens 23-26 7714802-11 1995 Specific [3H]alpha-trinositol binding sites may represent receptors by which alpha-trinositol inhibits NPY effects on vascular tone. Tritium 10-12 neuropeptide Y Homo sapiens 103-106 7718236-2 1995 Under serum-free culture conditions, insulin-like growth factor I (IGF-I), IGF-II, or insulin caused an increase in the proportion of cells that incorporated [3H]thymidine. Tritium 159-161 insulin like growth factor 1 Gallus gallus 37-65 7718236-2 1995 Under serum-free culture conditions, insulin-like growth factor I (IGF-I), IGF-II, or insulin caused an increase in the proportion of cells that incorporated [3H]thymidine. Tritium 159-161 insulin like growth factor 1 Gallus gallus 67-72 7617706-5 1995 The binding of the [3H]GBR 12935-labeled DA transporter was lower in the frontal cortex in the transgenic male TGF alpha mice than in the female TGF alpha mice. Tritium 20-22 transforming growth factor alpha Mus musculus 111-120 7617706-5 1995 The binding of the [3H]GBR 12935-labeled DA transporter was lower in the frontal cortex in the transgenic male TGF alpha mice than in the female TGF alpha mice. Tritium 20-22 transforming growth factor alpha Mus musculus 145-154 7606435-3 1995 The highest concentrations of [3H]WAY-100635-labelled sites were found in the lateral septal areas, dorsal raphe n., entorhinal cortex and the hippocampal formation (CA1, CA3 and dentate gyrus). Tritium 31-33 carbonic anhydrase 1 Rattus norvegicus 166-169 8758815-9 1996 The inhibitory rates of DNA synthesis of these tumor cells, measured by 3H-TdR incorporation tests, were greater in DDP plus CsA group than those in DDP alone group (64.7% 63.9% versus 39.6% 25.1%, P < 0.01). Tritium 72-74 translocase of inner mitochondrial membrane 8A Homo sapiens 116-119 8567030-4 1995 EP3 R expression was demonstrated by inhibition of [3H]PGE2 binding with the EP1/EP3 agonist sulprostone [50% inhibitory concentration (IC50 = 3.3 +/- 0.6 nM)] and the EP3/EP2 agonist M&B 28767 (IC50 = 2.1 +/- 0.3 nM), but not with the EP1 antagonist SC-19220. Tritium 52-54 prostaglandin E receptor 1 Homo sapiens 77-80 8567030-4 1995 EP3 R expression was demonstrated by inhibition of [3H]PGE2 binding with the EP1/EP3 agonist sulprostone [50% inhibitory concentration (IC50 = 3.3 +/- 0.6 nM)] and the EP3/EP2 agonist M&B 28767 (IC50 = 2.1 +/- 0.3 nM), but not with the EP1 antagonist SC-19220. Tritium 52-54 prostaglandin E receptor 1 Homo sapiens 240-243 7500171-4 1995 Following intraportal injection of a flooding dose of [3H]phenylalanine, in vivo hepatic apo A-I synthesis and secretion were significantly greater in the copper-deficient animals as detected by [3H]phenylalanine incorporation into immunoprecipitable apo A-I isolated from liver homogenates and plasma using anti-rat apo A-I antibodies. Tritium 55-57 apolipoprotein A1 Rattus norvegicus 251-258 3058540-11 1988 When the media were supplemented with growth factors, measurable increases in uptake of [3H]thymidine occurred with human epidermal growth factor (h-EGF), insulin-like growth factor-1 (IGF-1), nerve growth factor (NGF), transforming growth factor-beta (TGF-beta), bovine acidic fibroblast growth factor (baFGF), IL-1, bovine basic fibroblast growth factor (bbFGF), and Forskolin. Tritium 89-91 epidermal growth factor Homo sapiens 122-145 8531106-2 1995 BIII 277 CL potently displaced [3H]MK-801 binding from the NMDA receptor-channel complex in synaptosomal membrane preparations from rat brain cortex (Ki = 4.49 nmol/l). Tritium 32-34 calcium voltage-gated channel subunit alpha1 B Rattus norvegicus 0-4 7749347-2 1995 IGF-I, TGF beta, and insulin all stimulated [3H] leucine incorporation into protein; FGF, EGF, triiodothyronine and dexamethasone had little or no effect. Tritium 45-47 insulin-like growth factor 1 Rattus norvegicus 0-5 3058540-11 1988 When the media were supplemented with growth factors, measurable increases in uptake of [3H]thymidine occurred with human epidermal growth factor (h-EGF), insulin-like growth factor-1 (IGF-1), nerve growth factor (NGF), transforming growth factor-beta (TGF-beta), bovine acidic fibroblast growth factor (baFGF), IL-1, bovine basic fibroblast growth factor (bbFGF), and Forskolin. Tritium 89-91 interleukin 1 alpha Homo sapiens 312-316 7749347-4 1995 Only IGF-I caused substantial stimulation of [3H] thymidine incorporation; small effects of TGF beta, FGF and insulin were also seen. Tritium 46-48 insulin-like growth factor 1 Rattus norvegicus 5-10 8562170-4 1995 Increased growth in the presence of gastrin-17 (used at physiological concentrations and as assessed by [3H] thymidine uptake) was shown in 16/34 (47%) tumours. Tritium 105-107 gastrin Homo sapiens 36-43 2851638-9 1988 The time-course of inhibition of tritium labelled [D-Ala2-N-Me-Phe4,Gly5-ol]-enkephalin [( 3H]DAGO) binding (mu-opioid sites) revealed that naloxone occupies the mu-receptor for a relatively short period of time. Tritium 33-40 proenkephalin Rattus norvegicus 77-87 7595490-5 1995 Haloperidol increased the density of striatal A2a receptors by 33% (control, 522.4 +/- 20.7 fmol/mg of protein; haloperidol, 694.6 +/- 23.6 fmol/mg of protein; p < 0.001) without changing their affinity for [3H]CGS-21680 (control, 19.2 +/- 2.2 nM; haloperidol, 21.4 +/- 2.3 nM). Tritium 211-213 spectrin, alpha, non-erythrocytic 1 Rattus norvegicus 46-49 7595490-7 1995 Binding characteristics and the pharmacological displacement profile of the increased [3H]CGS-21680 binding sites confirmed them as A2a receptors. Tritium 87-89 spectrin, alpha, non-erythrocytic 1 Rattus norvegicus 132-135 7891319-6 1995 In binding experiments at human IM9 cells, the pseudopeptide compounds displaced [3H]substance P from human NK1 receptor, showing similar affinities to those displayed at the NK1 receptor in the guinea-pig ileum or bronchus against substance P methylester or septide, as agonists, respectively. Tritium 82-84 tachykinin receptor 1 Homo sapiens 108-120 2851638-9 1988 The time-course of inhibition of tritium labelled [D-Ala2-N-Me-Phe4,Gly5-ol]-enkephalin [( 3H]DAGO) binding (mu-opioid sites) revealed that naloxone occupies the mu-receptor for a relatively short period of time. Tritium 89-93 proenkephalin Rattus norvegicus 77-87 7538104-9 1995 When L1210 cells were co-cultured with peritoneal macrophages from mice intraperitoneally injected with hM-CSF, the uptake of [3H]thymidine in L1210 cells was inhibited. Tritium 127-129 colony stimulating factor 1 Homo sapiens 104-110 3141589-2 1988 [3H]5-HT binding, in the presence of pindolol (to block 5-HT1A and 5-HT1B receptors) and mesulergine (to block 5-HT1C receptors), was specific, saturable, reversible, and of high affinity. Tritium 1-3 5-hydroxytryptamine receptor 1A Homo sapiens 56-62 7772243-1 1995 Addition of LHRH for 40 h to aggregate cell cultures of 14-day-old rat pituitary significantly decreased the number of [3H]thymidine ([3H]T)-incorporating cells which immunostained for GH protein as well as the number of [3H]T-labelled cells expressing GH mRNA detectable by in situ hybridization with a digoxigenin-labelled riboprobe. Tritium 120-122 gonadotropin releasing hormone 1 Rattus norvegicus 12-16 7772243-1 1995 Addition of LHRH for 40 h to aggregate cell cultures of 14-day-old rat pituitary significantly decreased the number of [3H]thymidine ([3H]T)-incorporating cells which immunostained for GH protein as well as the number of [3H]T-labelled cells expressing GH mRNA detectable by in situ hybridization with a digoxigenin-labelled riboprobe. Tritium 135-137 gonadotropin releasing hormone 1 Rattus norvegicus 12-16 7772243-1 1995 Addition of LHRH for 40 h to aggregate cell cultures of 14-day-old rat pituitary significantly decreased the number of [3H]thymidine ([3H]T)-incorporating cells which immunostained for GH protein as well as the number of [3H]T-labelled cells expressing GH mRNA detectable by in situ hybridization with a digoxigenin-labelled riboprobe. Tritium 135-137 gonadotropin releasing hormone 1 Rattus norvegicus 12-16 7707306-1 1995 The abundance of mRNA encoding the PGF2 alpha receptor in bovine corpora lutea at different phases of the oestrous cycle and pregnancy was examined in relation to the number of [3H]PGF2 alpha binding sites. Tritium 178-180 prostaglandin F synthase 2 Bos taurus 35-39 7707306-4 1995 Specific high affinity [3H]PGF2 alpha binding sites with Kd values of 18.3-31.1 nmol-1 were observed in the luteal membrane during the oestrous cycle and pregnancy; this is consistent with the expression of PGF2 alpha receptor mRNA. Tritium 24-26 prostaglandin F synthase 2 Bos taurus 27-31 7802629-3 1994 Immunoprecipitation experiment of [3H]InsP4 binding activity of the purified protein using polyclonal antibody against the C2A domain of rat synaptotagmin II also revealed that mouse synaptotagmin II is the InsP4 binding protein (IP4BP). Tritium 35-37 synaptotagmin 2 Rattus norvegicus 141-157 7527004-11 1994 The combination of interferon-gamma and lipopolysaccharide was also found to inhibit Ito cell DNA synthesis, as measured on the basis of [3H]-thymidine uptake. Tritium 138-140 interferon gamma Rattus norvegicus 19-35 8868061-1 1995 Three days of treatment with the glucocorticoid dexamethasone (1 nM-1 microM) induced a concentration-dependent up-regulation of muscle nicotinic acetylcholine receptor (nAChR) in C2C12 mouse myotubes (EC50 = 10 +/- 7.3 nM), as assessed by [3H]alpha-BuTx binding. Tritium 241-243 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 170-175 7594622-2 1995 The CHO cells expressing the PAF receptor, termed CHO.1F8, showed a significant intracellular Ca2+ response to PAF, and the same binding properties to [3H]WEB 2086, a PAF antagonist, as reported (Kd, 13.6 +/- 1.9 nM; Bmax, 2.5 +/- 0.4 pmol/mg protein (n = 6)). Tritium 152-154 PCNA clamp associated factor Homo sapiens 29-32 8566176-1 1995 The apparent dissociation constant, Ki, for a neuroleptic at the dopamine D2 or D4 receptor was consistently higher when competed against [3H]nemonapride than against [3H]spiperone which was in turn higher than that against [3H]raclopride. Tritium 139-141 dopamine receptor D4 Homo sapiens 80-91 7589327-3 1995 The present studies employed experimental techniques to remove afferent axons or classes of projection neurons from rat ACC area 24 followed by coverslip autoradiography to localize changes in binding of [3H]Tyr-D-Ala-Gly-MePhe-Gly-ol (DAMGO) to mu receptors and 2-[3H]D-penicillamine-5-D-penicillamine-enkephalin (DPDPE) to delta receptors. Tritium 205-207 proenkephalin Rattus norvegicus 303-313 7707491-4 1994 d-Cloprostenol and PGF2 alpha were equipotent, about 150 times more potent than dl-cloprostenol (P < 0.05) and approximately 280 times more potent than PGE1 (P < 0.05) in inhibiting [3H]PGF2 alpha binding to corpus luteum cell membranes. Tritium 189-191 prostaglandin F synthase 2 Bos taurus 19-23 2854223-4 1988 The receptor antagonist [D-pGlu1,D-Phe2,D-Trp3,6]-luteinizing hormone-releasing hormone blocked the effect of GnRH on [3H]-inositol phosphate production. Tritium 119-123 gonadotropin releasing hormone 1 Rattus norvegicus 110-114 7882003-3 1994 [3H]PDBu binding in stratum oriens of the CA1 and CA3 sectors and stratum lucidum of the CA3 sector increased to adult levels by 4 weeks. Tritium 1-3 carbonic anhydrase 1 Rattus norvegicus 42-45 7882003-5 1994 Interestingly, in stratum lacunosum-moleculare of the CA1 sector, the [3H]PDBu binding activity was the highest at 1 week. Tritium 71-73 carbonic anhydrase 1 Rattus norvegicus 54-57 8608269-6 1995 The Man6GlcNAcGlcNAc[3H]ol isomers formed were easily defined by alpha 1,2-mannosidase sensitivity and either Bio-Gel P-4 gel filtration or AX-5 high-performance liquid chromatography. Tritium 21-23 solute carrier family 10 member 4 Homo sapiens 118-121 8567816-7 1995 CSF-1 was found to increase [3H]thymidine uptake by trophoblast cultured on laminin for 72 h, but no such response was seen in trophoblast cultured on fibronectin, indicating that these extracellular matrix proteins have differential effects on the response of trophoblast to this cytokine. Tritium 29-31 colony stimulating factor 1 Homo sapiens 0-5 8845736-6 1995 The density of agonist- and antagonist-binding sites in the CA1 region of the hippocampus were determined by autoradiography, using [3H]CGP39653 or [3H]glutamate as ligands. Tritium 133-135 carbonic anhydrase 1 Rattus norvegicus 60-63 8845736-6 1995 The density of agonist- and antagonist-binding sites in the CA1 region of the hippocampus were determined by autoradiography, using [3H]CGP39653 or [3H]glutamate as ligands. Tritium 149-151 carbonic anhydrase 1 Rattus norvegicus 60-63 2462415-4 1988 Several 3H-labelled cell proteins bound to active, but not to inactivated, alpha 2M. Tritium 8-10 alpha-2-macroglobulin Homo sapiens 75-83 7874313-5 1994 The [3H]noradrenaline release evoked by NMDA (100 microM)+gp120 (100 pM) was prevented by classical NMDA receptor antagonists, as well as by 10 microM memantine. Tritium 5-7 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 58-63 8548301-2 1995 The 3,4-DAP-evoked [3H]overflow was enhanced by the NO synthase substrate L-arginine, but not by D-arginine; it was reduced by the NO synthase inhibitor NG-nitro-L-arginine, which also antagonized the effects of L-arginine. Tritium 20-22 death-associated protein Rattus norvegicus 8-11 2854073-1 1988 The reversible acetylcholinesterase inhibitor, physostigmine, stimulated in a dose-dependent manner the accumulation of [3H]inositol monophosphate ([3H]IP1) in lithium-treated neostriatal slices. Tritium 121-123 acetylcholinesterase Rattus norvegicus 15-35 7498319-4 1995 [3H]5-CT appeared to label 5-HT1B/5-HT1D sites in the substantia nigra, globus pallidus and caudate/putamen, as the binding in these regions was displaced by the 5-HT1B/1D receptor selective agents sumatriptan, CP-122,288 and GR-127,935. Tritium 1-3 5-hydroxytryptamine receptor 1D Rattus norvegicus 34-40 7960581-4 1994 Interleukin-1-alpha (IL-1-alpha) was added to some cultures to examine its effect on MLCER: The lymphocyte responses were measured by 3H-thymidine uptake for the last 18 hours of incubation in MLCER: RESULTS: IFN-gamma-treated, HLA-class-II-bearing HCE cells stimulated allogenic lymphocytes, whereas IFN-gamma nontreated, class-II-negative HCE cells did not. Tritium 134-136 interleukin 1 alpha Homo sapiens 0-19 7829990-6 1994 In ROS 17/2.8 cells, IGF-I stimulated [3H]thymidine incorporation, after 22 h of treatment, in parallel with CK activity. Tritium 39-41 insulin-like growth factor 1 Rattus norvegicus 21-26 3262709-2 1988 It was observed that in most cases, rIL-2 used at optimal concentration induced higher levels of tritiated thymidine ([3H]TdR) uptake than rIL-4 used at optimal concentration. Tritium 119-121 interleukin 2 Rattus norvegicus 36-41 7938016-5 1994 The enzyme transferred [3H]methyl to geranylgeranylated Rab3A but not to geranylgeranylated Rab1A. Tritium 24-26 RAB3A, member RAS oncogene family Bos taurus 56-61 7492516-6 1995 In eight out of the 11 Tcc, the increase in [3H]thymidine incorporation and CD25 expression paralleled the increase of AgNOR area and number. Tritium 45-47 sideroflexin 1 Homo sapiens 23-26 8528563-6 1995 However, [3H]-U-46619 bound to A10 cells from passages 28-30 (p28-30) at a single class of site with a Bmax 111.0 +/- 9.0 fmol mg-1 protein and a KD value of 175.4 +/- 22.0 nM. Tritium 10-12 golgi SNAP receptor complex member 1 Rattus norvegicus 62-65 8528563-14 1995 The U-46619-induced increase of [3H]-thymidine incorporation into DNA of p28 -30 AO0 cells was potentiatedby PDGF (1 ng ml-1) and FCS (1%) and was inhibited by cinnamophilin (10 microM) and SQ29548 (1 microM)with estimated pKB values 5.4 +/- 1.2 and 6.3 +/- 0.9, respectively.6. Tritium 33-35 golgi SNAP receptor complex member 1 Rattus norvegicus 73-76 7565637-3 1995 We report that tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 alpha (IL-1 alpha) markedly stimulate glucose uptake and phosphorylation in primary cultures of neonatal murine astrocytes, as determined with [3H]-2-deoxyglucose ([3H]2DG). Tritium 217-219 interleukin 1 alpha Mus musculus 59-78 7565637-3 1995 We report that tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 alpha (IL-1 alpha) markedly stimulate glucose uptake and phosphorylation in primary cultures of neonatal murine astrocytes, as determined with [3H]-2-deoxyglucose ([3H]2DG). Tritium 217-219 interleukin 1 alpha Mus musculus 80-90 7565637-3 1995 We report that tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 alpha (IL-1 alpha) markedly stimulate glucose uptake and phosphorylation in primary cultures of neonatal murine astrocytes, as determined with [3H]-2-deoxyglucose ([3H]2DG). Tritium 238-240 interleukin 1 alpha Mus musculus 59-78 7565637-3 1995 We report that tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 alpha (IL-1 alpha) markedly stimulate glucose uptake and phosphorylation in primary cultures of neonatal murine astrocytes, as determined with [3H]-2-deoxyglucose ([3H]2DG). Tritium 238-240 interleukin 1 alpha Mus musculus 80-90 7565637-6 1995 Evidence in support of this includes (i) inhibition by mepacrine, a phospholipase A2 inhibitor, of [3H]2DG uptake evoked by TNF-alpha and IL-1 alpha, and (ii) stimulation of [3H]arachidonic acid release by TNF-alpha and IL-1 alpha. Tritium 100-103 interleukin 1 alpha Mus musculus 138-148 7565637-6 1995 Evidence in support of this includes (i) inhibition by mepacrine, a phospholipase A2 inhibitor, of [3H]2DG uptake evoked by TNF-alpha and IL-1 alpha, and (ii) stimulation of [3H]arachidonic acid release by TNF-alpha and IL-1 alpha. Tritium 100-102 interleukin 1 alpha Mus musculus 138-148 7820685-5 1994 injection of [3H]Trp-P-1 (100 micrograms/kg or 1.5 mg/kg) in mice treated with the P4501A-inducing agent beta-naphthoflavone (BNF). Tritium 14-16 natriuretic peptide type B Mus musculus 105-130 7820685-8 1994 At incubation of brain slices with [3H]Trp-P-1, there was a marked irreversible binding of radioactivity in endothelial cells in the choroid plexus of BNF-treated mice but not in vehicle-treated control mice. Tritium 36-38 natriuretic peptide type B Mus musculus 151-154 7925644-4 1994 EGF and FGF-1 stimulated in both models under serum-free conditions the BrdU and [3H]-thymidine incorporation. Tritium 82-84 epidermal growth factor Mus musculus 0-3 7845062-7 1994 Light microscope autoradiography revealed a loss of silver grains developed after exposure of sections of hippocampus to [3H]pirenzepine in the stratum oriens of CA1 and CA3 fields in rats of 12 and 27 months in comparison with young animals. Tritium 122-124 carbonic anhydrase 1 Rattus norvegicus 162-165 2458432-5 1988 Phospholipase A2 treatment of cortical membrane preparations was equipotent in preventing the stimulatory effect of chloride on both [3H]DMCM and [3H]FNZ binding. Tritium 134-136 phospholipase A2 group IB Rattus norvegicus 0-16 7929093-9 1994 Using a solid-phase assay, we show that [3H]suramin specifically binds to recombinant gp120 and that this binding could be blocked by a monoclonal antibody specific for the conserved GPGRAF motif of the V3 domain of gp120. Tritium 41-43 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 86-91 7627714-8 1995 In normal but not hyper-apoB cells, genistein inhibited the significant stimulation by BP I of the rates of both [14C]acetate (P = .0001) and [3H]mevalonolactone (P = .04) incorporation into unesterified cholesterol. Tritium 143-145 bactericidal permeability increasing protein Homo sapiens 87-91 7929093-9 1994 Using a solid-phase assay, we show that [3H]suramin specifically binds to recombinant gp120 and that this binding could be blocked by a monoclonal antibody specific for the conserved GPGRAF motif of the V3 domain of gp120. Tritium 41-43 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 216-221 2458432-5 1988 Phospholipase A2 treatment of cortical membrane preparations was equipotent in preventing the stimulatory effect of chloride on both [3H]DMCM and [3H]FNZ binding. Tritium 147-149 phospholipase A2 group IB Rattus norvegicus 0-16 7622582-5 1995 By comparison, insulin-like growth factor (IGF-I) has a small effect on [3H]-thymidine incorporation. Tritium 73-75 insulin-like growth factor 1 Rattus norvegicus 43-48 3414808-5 1988 The bulk of the tritium that accumulated in the cells was associated with intact [3H3C]Alb. Tritium 16-23 albumin Oryctolagus cuniculus 87-90 7622582-16 1995 PDGF or PDGF plus IGF-I increased [3H]-thymidine incorporation in stromal cells from old rats but to a lesser extent. Tritium 35-37 insulin-like growth factor 1 Rattus norvegicus 18-23 7622585-10 1995 Both primary cultures and BGC-1 cells exhibited a stimulatory response to EGF and IGF-I on [3H]-thymidine incorporation. Tritium 92-94 insulin like growth factor 1 Bos taurus 82-87 8570860-8 1995 A summary of the findings is as follows: (1) IGF-I stimulates [3H]thy, LI and cAMP accumulation. Tritium 63-65 insulin-like growth factor 1 Rattus norvegicus 45-50 8080280-11 1994 Under conditions that led to cross-linking, rabbit triosephosphate isomerase took up 2.7 mol of 3H from NaB3H4/mol dimer, and the yeast enzyme incorporated only 0.4 mol of 3H. Tritium 96-98 triosephosphate isomerase Oryctolagus cuniculus 51-76 8080280-11 1994 Under conditions that led to cross-linking, rabbit triosephosphate isomerase took up 2.7 mol of 3H from NaB3H4/mol dimer, and the yeast enzyme incorporated only 0.4 mol of 3H. Tritium 107-109 triosephosphate isomerase Oryctolagus cuniculus 51-76 2841099-7 1988 In contrast, the independent stimulatory effects of TPA and insulin-like growth factor I (IGF-I) on [3H]thymidine incorporation and cell replication were at least additive when the two stimulators were added together. Tritium 101-103 insulin-like growth factor 1 Rattus norvegicus 60-88 8078092-4 1994 Concentrations of paraoxon > 0.5 x 10(-3) M and PSP 1 x 10(-3) M significantly inhibited the binding of a saturating concentration of 3H-NMS. Tritium 137-139 paraspeckle component 1 Homo sapiens 51-56 8570860-9 1995 (2) IGF-II stimulates [3H]thy and LI but not cAMP; (3) IGF-I but not IGF-II effects are inhibited by SS14 and DDA. Tritium 23-25 insulin-like growth factor 1 Rattus norvegicus 4-9 7619858-14 1995 In the presence of apo A-II, enhanced [3H]cholesterol delivery to cells was seen which could contribute to the proatherogenic activity of apo A-II. Tritium 39-41 apolipoprotein A2 Homo sapiens 19-27 7619858-14 1995 In the presence of apo A-II, enhanced [3H]cholesterol delivery to cells was seen which could contribute to the proatherogenic activity of apo A-II. Tritium 39-41 apolipoprotein A2 Homo sapiens 138-146 2841099-7 1988 In contrast, the independent stimulatory effects of TPA and insulin-like growth factor I (IGF-I) on [3H]thymidine incorporation and cell replication were at least additive when the two stimulators were added together. Tritium 101-103 insulin-like growth factor 1 Rattus norvegicus 90-95 7806940-3 1994 In HSC-1 and NA cells, which contain the highest number of EGF receptor among these SCC cell lines, the inhibition of 3H-thymidine incorporation was apparent 2 to 4 hours after treatment with 100 ng/ml of EGF and reached more than 95% inhibition after 24 hours. Tritium 118-120 epidermal growth factor Homo sapiens 59-62 7806940-3 1994 In HSC-1 and NA cells, which contain the highest number of EGF receptor among these SCC cell lines, the inhibition of 3H-thymidine incorporation was apparent 2 to 4 hours after treatment with 100 ng/ml of EGF and reached more than 95% inhibition after 24 hours. Tritium 118-120 epidermal growth factor Homo sapiens 205-208 7816197-7 1994 [3H]Ro41-1049 binding (i.e. monoamine oxidase A) was unchanged in all tissues of diseased versus control brains. Tritium 1-3 monoamine oxidase A Homo sapiens 28-47 7529387-4 1994 Binding of [3H]SP to mouse spinal cord membranes was inhibited by SP, CP-96,345, and to a lesser extent by SP(5-11), but not SP(1-7), consistent with these binding sites being NK-1 receptors. Tritium 12-14 tachykinin 1 Mus musculus 15-17 7529387-4 1994 Binding of [3H]SP to mouse spinal cord membranes was inhibited by SP, CP-96,345, and to a lesser extent by SP(5-11), but not SP(1-7), consistent with these binding sites being NK-1 receptors. Tritium 12-14 tachykinin 1 Mus musculus 66-68 7647015-3 1995 Here we demonstrate that the AML1/EVI-1-specific antisense oligonucleotide markedly decreases the [3H]thymidine incorporation and growth of leukaemic cells carrying the t(3;21) and the t(9;22), but not those of K562 cells. Tritium 99-101 RUNX family transcription factor 1 Homo sapiens 29-33 7647015-3 1995 Here we demonstrate that the AML1/EVI-1-specific antisense oligonucleotide markedly decreases the [3H]thymidine incorporation and growth of leukaemic cells carrying the t(3;21) and the t(9;22), but not those of K562 cells. Tritium 99-101 RUNX family transcription factor 1 Homo sapiens 34-39 7577807-8 1995 Furthermore, when recombinant ADF (rADF) was added to a culture of PBMC 1 h before the addition of PHA and FK506, the action of FK506 was partially reversed as determined by [3H]thymidine incorporation and viable cell counts. Tritium 175-177 thioredoxin Homo sapiens 30-33 7529387-4 1994 Binding of [3H]SP to mouse spinal cord membranes was inhibited by SP, CP-96,345, and to a lesser extent by SP(5-11), but not SP(1-7), consistent with these binding sites being NK-1 receptors. Tritium 12-14 tachykinin 1 Mus musculus 66-68 2901965-2 1988 Low doses of IL2 (0.1-1.0 U/ml) induce clone 5.8.6 to kill in an antigen-independent fashion in short-term 51Cr-release and 3H-release assays (6-12 h). Tritium 124-126 interleukin 2 Mus musculus 13-16 7521134-15 1994 In addition, activin A potently inhibited DNA synthesis as assessed by [3H]thymidine incorporation. Tritium 72-74 inhibin subunit beta A Rattus norvegicus 13-22 7775603-8 1995 In addition to its synergism with IGF-I, preincubation with dexamethasone augmented insulin and epidermal growth factor (EGF) stimulation of [3H]thymidine incorporation; dexamethasone had no effect on platelet-derived growth factor or fibroblast growth factor action. Tritium 142-144 epidermal growth factor Homo sapiens 96-119 7775603-8 1995 In addition to its synergism with IGF-I, preincubation with dexamethasone augmented insulin and epidermal growth factor (EGF) stimulation of [3H]thymidine incorporation; dexamethasone had no effect on platelet-derived growth factor or fibroblast growth factor action. Tritium 142-144 epidermal growth factor Homo sapiens 121-124 7519973-1 1994 [3H]Aflatoxin B1-8,9-epoxide ([3H]AFB1-8,9-epoxide), the putative ultimate carcinogen of AFB1, was synthesized and tested for its binding specificity to and transcriptional effect on several single- and double-stranded DNAs containing cytosine. Tritium 1-3 NADH:ubiquinone oxidoreductase subunit B7 Homo sapiens 14-18 3208103-3 1988 Five days after unilateral CA3 lesioning, a reduction of [3H]CHA binding by 10-30% of ipsi- and contralateral was observed in the dendritic fields in the CA1 region, though a significant reduction was restricted to the ipsilateral stratum radiatum. Tritium 58-60 carbonic anhydrase 1 Rattus norvegicus 154-157 7519973-1 1994 [3H]Aflatoxin B1-8,9-epoxide ([3H]AFB1-8,9-epoxide), the putative ultimate carcinogen of AFB1, was synthesized and tested for its binding specificity to and transcriptional effect on several single- and double-stranded DNAs containing cytosine. Tritium 31-33 NADH:ubiquinone oxidoreductase subunit B7 Homo sapiens 14-18 7922585-1 1994 Addition of endothelin-1 to cultured rat brain capillary endothelial cells induced a 2.7-fold activation of phospholipase A2, as evidenced from the release of [3H]arachidonic acid from prelabelled cells. Tritium 160-162 phospholipase A2 group IB Rattus norvegicus 108-124 7921158-1 1994 Epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), and insulin all dose-dependently stimulated [3H]-thymidine incorporation into guinea pig gastric mucous cells cultured in vitro. Tritium 115-117 pro-epidermal growth factor Cavia porcellus 0-23 7921158-1 1994 Epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), and insulin all dose-dependently stimulated [3H]-thymidine incorporation into guinea pig gastric mucous cells cultured in vitro. Tritium 115-117 pro-epidermal growth factor Cavia porcellus 25-28 7538120-3 1995 In this study, we show that the E-selectin receptor-globulin (E-selectin Rg) affinity chromatography can isolate specifically only one glycoprotein from [3H]glucosamine-labeled HL-60 cells in a Ca(2+)-dependent manner. Tritium 154-156 selectin E Homo sapiens 32-42 7538120-3 1995 In this study, we show that the E-selectin receptor-globulin (E-selectin Rg) affinity chromatography can isolate specifically only one glycoprotein from [3H]glucosamine-labeled HL-60 cells in a Ca(2+)-dependent manner. Tritium 154-156 selectin E Homo sapiens 62-72 7762602-3 1995 TNF increased the diffusional transit of [3H]sorbitol, [3H]inulin, and 125I-labeled albumin across confluent bovine aortic EC monolayers. Tritium 42-44 tumor necrosis factor Bos taurus 0-3 7786802-6 1995 Cell proliferative capacity was well correlated with the amount of cdc2 mRNA transcripts, i.e. 3H-thymidine incorporation was highest in cases with cdc2 mRNA over-expression and was significantly higher in cdc2-positive cases than in cdc2-negative cases. Tritium 95-97 cyclin dependent kinase 1 Homo sapiens 67-71 3208103-4 1988 Thirty-five days after CA3 lesioning, [3H]CHA binding in the stratum radiatum in the CA1 subfield ipsilateral to the CA3 lesion revealed a small reduction in Bmax values but no alteration in Bmax in other sublayers of the CA1. Tritium 39-41 carbonic anhydrase 1 Rattus norvegicus 85-88 7786802-6 1995 Cell proliferative capacity was well correlated with the amount of cdc2 mRNA transcripts, i.e. 3H-thymidine incorporation was highest in cases with cdc2 mRNA over-expression and was significantly higher in cdc2-positive cases than in cdc2-negative cases. Tritium 95-97 cyclin dependent kinase 1 Homo sapiens 148-152 7786802-6 1995 Cell proliferative capacity was well correlated with the amount of cdc2 mRNA transcripts, i.e. 3H-thymidine incorporation was highest in cases with cdc2 mRNA over-expression and was significantly higher in cdc2-positive cases than in cdc2-negative cases. Tritium 95-97 cyclin dependent kinase 1 Homo sapiens 148-152 7786802-6 1995 Cell proliferative capacity was well correlated with the amount of cdc2 mRNA transcripts, i.e. 3H-thymidine incorporation was highest in cases with cdc2 mRNA over-expression and was significantly higher in cdc2-positive cases than in cdc2-negative cases. Tritium 95-97 cyclin dependent kinase 1 Homo sapiens 148-152 8207430-6 1994 Both adenosine deaminase (2 U/ml) and DPCPX (250 nM) increased the evoked release of [3H]ACh in the CA1 and CA3 areas but not in the dentate gyrus. Tritium 86-88 carbonic anhydrase 1 Rattus norvegicus 100-103 3208103-6 1988 Selective CA1 pyramidal cell lesioning by transient ischemia caused a 70% reduction of [3H]CHA binding sites in the CA1 subfield. Tritium 88-90 carbonic anhydrase 1 Rattus norvegicus 10-13 3208103-6 1988 Selective CA1 pyramidal cell lesioning by transient ischemia caused a 70% reduction of [3H]CHA binding sites in the CA1 subfield. Tritium 88-90 carbonic anhydrase 1 Rattus norvegicus 116-119 2845945-9 1988 In contrast with previous observations in human A431 cells, the concentration-dependence of the EGF-triggered [Ca2+]i increase in EGFR T17 cells paralleled that of [3H]thymidine incorporation. Tritium 165-167 epidermal growth factor Homo sapiens 96-99 7515814-5 1994 Unlike CD5- B cells, CD5+ B cells proliferated vigorously in response to PMA as assessed by [3H]thymidine incorporation and cell cycle analysis in vitro. Tritium 93-95 CD5 molecule Homo sapiens 21-24 7952273-8 1994 These differences prompted the further characterization of [3H]SR 95531 binding in the LC, revealing a significant affinity for monoamine oxidase type A (MAO-A), which is highly concentrated in this nucleus. Tritium 60-62 monoamine oxidase A Homo sapiens 154-159 7952273-9 1994 In a competition binding study, a reduction of up to 25% of the [3H]SR 95531 binding was observed with MAO-A but not MAO-B inhibitors, at concentrations which produce maximum but selective enzyme inhibition. Tritium 65-67 monoamine oxidase A Homo sapiens 103-108 7952273-11 1994 Moreover, enzyme radioautography with [3H]Ro 41-1049 revealed that SR 95531 has a significant affinity for MAO-A (IC50 values were 10(-5) and 4 x 10(-6) M in the LC and dentate gyrus respectively) but not for MAO-B ([3H]lazabemide binding). Tritium 217-219 monoamine oxidase A Homo sapiens 107-112 8206710-4 1994 The effect of activin A on DNA synthesis was studied by measuring (3H) thymidine incorporation after cells were exposed to recombinant human activin A (rhA). Tritium 67-69 inhibin subunit beta E Homo sapiens 14-21 7636797-3 1995 Concentrations of 0.16, 1.6 and 16 nmol EGF l-1 significantly increased both [3H]thymidine (P < 0.01) and [3H]leucine incorporation (P < 0.01). Tritium 78-80 epidermal growth factor Homo sapiens 40-43 7636797-3 1995 Concentrations of 0.16, 1.6 and 16 nmol EGF l-1 significantly increased both [3H]thymidine (P < 0.01) and [3H]leucine incorporation (P < 0.01). Tritium 110-112 epidermal growth factor Homo sapiens 40-43 7627803-5 1995 Both natural and recombinant type 1 IFNs inhibited TCGF-induced tritiated thymidine (3H-TdR) incorporation in the wild-type T cell clone, with a ID50 of 60-80 U/ml. Tritium 85-87 interleukin 2 Mus musculus 51-55 7695632-4 1995 Association of CaM with activated [3H]E2-ER complexes increased the ability of the latter to interact with an estrogen response element (ERE) as demonstrated by DNA-cellulose competition assay; CaM also reinforces the potency of ERE to dissociate the [3H]E2-ER complex. Tritium 35-37 estrogen receptor 1 Rattus norvegicus 41-43 7695632-4 1995 Association of CaM with activated [3H]E2-ER complexes increased the ability of the latter to interact with an estrogen response element (ERE) as demonstrated by DNA-cellulose competition assay; CaM also reinforces the potency of ERE to dissociate the [3H]E2-ER complex. Tritium 252-254 estrogen receptor 1 Rattus norvegicus 41-43 7890662-7 1995 Following growth of HL-60 cells with [3H]MTX, used as a model folate compound, a large reduction in its intracellular polyglutamate pools was shown during maturation which quantitatively reflected the decline in FPGS activity as well as folate transport inward (Sirotnak, F.M., Jacobson, D.M., and Yang, C-H. (1986) J. Biol. Tritium 38-40 folylpolyglutamate synthase Homo sapiens 212-216 8089198-5 1994 At 3 h after the treatment of the cells with H-7 and TPA, vitamin D receptor (VDR) contents estimated by 3H-1,25(OH)2D3 binding capacity were 72.4 and 63.2% of vehicle-treated cells without significant changes of binding affinities, suggesting that the effect of H-7 and TPA was not the result of changes in VDR content or its binding affinity. Tritium 105-107 vitamin D receptor Rattus norvegicus 58-76 8089198-5 1994 At 3 h after the treatment of the cells with H-7 and TPA, vitamin D receptor (VDR) contents estimated by 3H-1,25(OH)2D3 binding capacity were 72.4 and 63.2% of vehicle-treated cells without significant changes of binding affinities, suggesting that the effect of H-7 and TPA was not the result of changes in VDR content or its binding affinity. Tritium 105-107 vitamin D receptor Rattus norvegicus 78-81 7696150-13 1995 Using rat uterine cytosol as a source of estrogen receptor, the compounds displaced only a small percentage of [3H]estradiol binding, even when present at 1000-fold excess. Tritium 112-114 estrogen receptor 1 Rattus norvegicus 41-58 3260173-3 1988 EGF, but neither Sm-C nor TGFb, alone consistently stimulated, in a dose-dependent manner, [3H]thymidine incorporation by porcine granulosa cells under defined conditions (P less than 0.01). Tritium 92-94 epidermal growth factor Homo sapiens 0-3 7848283-3 1995 We report here that differentiation of a human eosinophilic leukaemia cell line, EoL-1, by sodium n-butyrate is associated with induction of PAF receptor gene expression, as indicated by: PAF receptor mRNA accumulation; increases in the binding of [3H]WEB 2086, a PAF antagonist; analysis of cell-surface expression of PAF receptor protein using a monoclonal anti-(PAF receptor) antibody; and augmentation of PAF-induced increase in the intracellular concentration of calcium. Tritium 249-251 PCNA clamp associated factor Homo sapiens 141-144 7967223-0 1994 Binding characteristics of a histamine H3-receptor antagonist, [3H]S-methylthioperamide: comparison with [3H](R)alpha-methylhistamine binding to rat tissues. Tritium 64-66 histamine receptor H3 Rattus norvegicus 29-50 7967223-3 1994 Here we describe the binding characteristics of a new histamine H3-receptor antagonist, [3H]S-methylthioperamide (SMT), to rat tissues, and compare its binding with that of [3H](R)alpha-methylhistamine ((R)alpha MH), a selective histamine H3-receptor agonist. Tritium 89-91 histamine receptor H3 Rattus norvegicus 54-75 3260173-4 1988 Sm-C (10 ng/ml) and TGFb (1 ng/ml) both enhanced EGF-stimulated [3H]thymidine incorporation (56% and 300%, respectively; P less than 0.05). Tritium 65-67 epidermal growth factor Homo sapiens 49-52 8188084-2 1994 EGF (10 ng/ml) and the tumor promoter, protein kinase C agonist, phorbol 12-myristate 13-acetate (PMA) were potent stimulators (P < 0.01) of DNA synthesis in this cell line as determined by [3H]thymidine incorporation into DNA. Tritium 194-196 epidermal growth factor Homo sapiens 0-3 7773125-10 1995 The 3H-thymidine labeling index of each cell type of male mouse submandibular glands reached a peak 1 day after birth and decreased with aging. Tritium 4-6 pseudopodium-enriched atypical kinase 1 Mus musculus 95-101 8188084-3 1994 Staurosporine, a protein kinase C inhibitor, partially blocked the EGF effects on [3H]thymidine incorporation. Tritium 83-85 epidermal growth factor Homo sapiens 67-70 3260173-6 1988 When EGF, Sm-C, and TGFb were combined, [3H]thymidine incorporation was equivalent to that obtained with EGF plus 10% fetal bovine serum, heretofore the most potent stimulatory combination for [3H]thymidine incorporation. Tritium 41-43 epidermal growth factor Homo sapiens 5-8 3260173-6 1988 When EGF, Sm-C, and TGFb were combined, [3H]thymidine incorporation was equivalent to that obtained with EGF plus 10% fetal bovine serum, heretofore the most potent stimulatory combination for [3H]thymidine incorporation. Tritium 194-196 epidermal growth factor Homo sapiens 5-8 7537550-3 1995 Culture of G-CSF receptor-expressing cells (GR-K562) in the presence of G-CSF enhanced DNA synthesis and a stimulation index of 4.61 was obtained in a 3H-thymidine uptake assay. Tritium 151-153 colony stimulating factor 3 receptor Homo sapiens 11-25 7537550-3 1995 Culture of G-CSF receptor-expressing cells (GR-K562) in the presence of G-CSF enhanced DNA synthesis and a stimulation index of 4.61 was obtained in a 3H-thymidine uptake assay. Tritium 151-153 colony stimulating factor 3 Homo sapiens 11-16 8175695-6 1994 The possibility that [3H]LTC4 specific binding was to a member of the glutathione S-transferase (GST) family of enzymes, such as LTC4 synthase, cytosolic GST, or microsomal GST, was therefore investigated. Tritium 22-24 leukotriene C4 synthase Homo sapiens 129-142 8144596-2 1994 We have isolated a Saccharomyces cerevisiae GPI anchoring mutant, gpi1, using a colony screen for cells blocked in [3H]inositol incorporation into protein. Tritium 116-118 phosphatidylinositol N-acetylglucosaminyltransferase Saccharomyces cerevisiae S288C 66-70 8026542-5 1994 Our results demonstrated that (1) high-affinity neurotensin binding sites are present in the guinea-pig brain in regions rich in both dopamine cell bodies and terminals; (2) the binding of neurotensin is inhibited by SR 48692 and its related S(+) active analog, SR 48527, with IC50 values in the nM range and (3) the non-peptide antagonist has no agonist effect but antagonizes neurotensin-induced [3H]dopamine release from guinea-pig striatal nerve terminals. Tritium 399-401 neurotensin/neuromedin N Cavia porcellus 48-59 7846033-5 1995 Characterization of apoB-41-associated lipids after labeling C127B-41 cells with [3H]oleate and immunoprecipitating the secreted LPs with antibodies to apoB showed that 3H-labeled triacylglycerols were a major lipid class and accounted for about 54% of the total labeled lipids. Tritium 82-84 apolipoprotein B Mus musculus 20-24 7846033-5 1995 Characterization of apoB-41-associated lipids after labeling C127B-41 cells with [3H]oleate and immunoprecipitating the secreted LPs with antibodies to apoB showed that 3H-labeled triacylglycerols were a major lipid class and accounted for about 54% of the total labeled lipids. Tritium 169-171 apolipoprotein B Mus musculus 20-24 7846033-5 1995 Characterization of apoB-41-associated lipids after labeling C127B-41 cells with [3H]oleate and immunoprecipitating the secreted LPs with antibodies to apoB showed that 3H-labeled triacylglycerols were a major lipid class and accounted for about 54% of the total labeled lipids. Tritium 169-171 apolipoprotein B Mus musculus 152-156 8026542-5 1994 Our results demonstrated that (1) high-affinity neurotensin binding sites are present in the guinea-pig brain in regions rich in both dopamine cell bodies and terminals; (2) the binding of neurotensin is inhibited by SR 48692 and its related S(+) active analog, SR 48527, with IC50 values in the nM range and (3) the non-peptide antagonist has no agonist effect but antagonizes neurotensin-induced [3H]dopamine release from guinea-pig striatal nerve terminals. Tritium 399-401 neurotensin/neuromedin N Cavia porcellus 189-200 2901831-7 1988 SDS/polyacrylamide-gel electrophoresis under non-reducing and reducing conditions revealed that the [3H]Pri2-P-protein was exclusively represented by a polypeptide of Mr 55,000. Tritium 101-103 oculocutaneous albinism II Mus musculus 109-118 8026542-5 1994 Our results demonstrated that (1) high-affinity neurotensin binding sites are present in the guinea-pig brain in regions rich in both dopamine cell bodies and terminals; (2) the binding of neurotensin is inhibited by SR 48692 and its related S(+) active analog, SR 48527, with IC50 values in the nM range and (3) the non-peptide antagonist has no agonist effect but antagonizes neurotensin-induced [3H]dopamine release from guinea-pig striatal nerve terminals. Tritium 399-401 neurotensin/neuromedin N Cavia porcellus 189-200 3376112-2 1988 Tritium-labeled T-2 toxin was injected into the tied-off intestinal segments at a dose of 5 or 500 nmol, respectively. Tritium 0-7 brachyury 2 Rattus norvegicus 16-19 8027338-5 1994 P22 is processed as a lipoprotein, as demonstrated by [3H]palmitate labeling. Tritium 55-57 calcineurin like EF-hand protein 1 Homo sapiens 0-3 8040840-9 1994 IGF-I, IGF-II and insulin each enhanced 2[3H]DG transport in a dose-dependent fashion. Tritium 42-44 insulin like growth factor 2 Homo sapiens 7-13 3391615-3 1988 Regional mapping by in situ hybridization of a 3H-labelled human G6S cDNA probe to human metaphase chromosomes indicated that the G6S gene is localized to chromosome 12 at q14. Tritium 47-49 glucosamine (N-acetyl)-6-sulfatase Homo sapiens 65-68 7510961-1 1994 Monocyte Chemotactic Protein(MCP)-1 and other members of the C-C branch of the chemokine superfamily (RANTES, MIP1 alpha/LD78, and MCP-3) induced, at chemotactic concentrations, the release of [3H]arachidonic acid in prelabeled human monocytes. Tritium 194-196 C-C motif chemokine ligand 2 Homo sapiens 9-35 7838530-6 1995 On the other hand, expression of a temperature-sensitive (ts) p53 gene in a partially-responsive rat line demonstrated a highly significant modulation of TGF beta response, which fell from 65% inhibition of 3H-thymidine labelling index at 32.5 degrees C (wt p53 conformation) to only 14% at 37.5 degrees C (mutant conformation). Tritium 207-209 Wistar clone pR53P1 p53 pseudogene Rattus norvegicus 62-65 7840652-4 1995 In MCF-7 cells cotreated with flavones 1 or 2 (0.01 to 10 microM) plus TCDD or [3H]TCDD, there was a concentration-dependent inhibition of TCDD-induced CYP1A1 mRNA levels and formation of radiolabeled nuclear Ah receptor complex. Tritium 80-82 cytochrome P450 family 1 subfamily A member 1 Homo sapiens 152-158 3391615-3 1988 Regional mapping by in situ hybridization of a 3H-labelled human G6S cDNA probe to human metaphase chromosomes indicated that the G6S gene is localized to chromosome 12 at q14. Tritium 47-49 glucosamine (N-acetyl)-6-sulfatase Homo sapiens 130-133 8016410-2 1994 NPY stimulated DNA synthesis in human SMC grown from subcutaneous arteries and veins (diameter: 0.4 mm) measured by [3H]thymidine incorporation. Tritium 117-119 neuropeptide Y Homo sapiens 0-3 7842483-2 1995 Cathepsin G increased the [3H]thymidine incorporation into human lymphocytes. Tritium 27-29 cathepsin G Homo sapiens 0-11 2453561-5 1988 [3H] Collagen, treated with tryptase-activated latent collagenase, was subjected to electrophoresis on SDS polyacrylamide gels and autoradiography showed the collagen degradation pattern (A, B) characteristically produced by collagenase. Tritium 1-3 tryptase alpha/beta 1 Mus musculus 28-36 7704621-3 1994 Ibotenic acid lesion resulted in a striking loss of acetylcholinesterase (AChE) staining in the lesioned Nbm which is associated with a 60% decrease in AChE staining and a 30% reduction in [3H]hemicholinium-3 binding in frontal and parietal cortical regions as well fore- and hindlimb areas ipsilateral to the lesion, being more prominent in the more rostral cortical regions. Tritium 190-192 acetylcholinesterase Rattus norvegicus 52-72 8118633-9 1994 We found that the time required for deacetylation of 50% of [3H]PAF (t1/2) to lyso-PAF was 50 min in baseline secretions and 10 and 22 min in NLF obtained 10 min and 24 h after antigen challenge, respectively. Tritium 61-63 PCNA clamp associated factor Homo sapiens 64-67 8118633-9 1994 We found that the time required for deacetylation of 50% of [3H]PAF (t1/2) to lyso-PAF was 50 min in baseline secretions and 10 and 22 min in NLF obtained 10 min and 24 h after antigen challenge, respectively. Tritium 61-63 PCNA clamp associated factor Homo sapiens 83-86 7704621-3 1994 Ibotenic acid lesion resulted in a striking loss of acetylcholinesterase (AChE) staining in the lesioned Nbm which is associated with a 60% decrease in AChE staining and a 30% reduction in [3H]hemicholinium-3 binding in frontal and parietal cortical regions as well fore- and hindlimb areas ipsilateral to the lesion, being more prominent in the more rostral cortical regions. Tritium 190-192 acetylcholinesterase Rattus norvegicus 74-78 3390445-4 1988 After treatment with endo-beta-galactosidase, 40-45% of the [3H]glucosamine or [3H]fucose radioactivity in the phospholipase-released glycoproteins was converted to products of disaccharide size, and the molecular size of the 158-kDa glycoprotein decreased to 145 kDa, demonstrating that it contains fucosylated poly-(N-acetyllactosaminyl) oligosaccharides. Tritium 61-63 galactosidase, beta 1 Rattus norvegicus 26-44 7705452-9 1994 Substantial (77%) blockade of [3H]WAY-100635 binding was achieved with the 5-HT1A receptor agonist, 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT), and the partial agonists, ipsapirone and buspirone. Tritium 31-33 5-hydroxytryptamine receptor 1A Homo sapiens 75-81 7987844-9 1994 We conclude from the mutual cross-resistance to DDP and antimony potassium tartrate and from the impaired uptake of [3H]DEP in both the DDP and antimony-selected variants that DDP and antimony share a common mechanism of resistance. Tritium 117-119 translocase of inner mitochondrial membrane 8A Homo sapiens 136-139 7987844-9 1994 We conclude from the mutual cross-resistance to DDP and antimony potassium tartrate and from the impaired uptake of [3H]DEP in both the DDP and antimony-selected variants that DDP and antimony share a common mechanism of resistance. Tritium 117-119 translocase of inner mitochondrial membrane 8A Homo sapiens 136-139 8031877-1 1994 3-Azibutyl (2R*,3S*)-2,3-bis(4-hydroxyphenyl)pentyl sulfide (1), a photoaffinity labeling reagent for the estrogen receptor (ER), has been prepared in unlabeled and in high specific activity tritium-labeled form (32 Ci/mmol) and has been shown to undergo selective and efficient photocovalent attachment to rat uterine ER. Tritium 191-198 estrogen receptor 1 Rattus norvegicus 106-123 8031877-1 1994 3-Azibutyl (2R*,3S*)-2,3-bis(4-hydroxyphenyl)pentyl sulfide (1), a photoaffinity labeling reagent for the estrogen receptor (ER), has been prepared in unlabeled and in high specific activity tritium-labeled form (32 Ci/mmol) and has been shown to undergo selective and efficient photocovalent attachment to rat uterine ER. Tritium 191-198 estrogen receptor 1 Rattus norvegicus 125-127 8031877-5 1994 The tritium-labeled diazirine [3H]-1 undergoes specific photocovalent attachment to ER with an attachment efficiency of 29% and a selectivity of 90%. Tritium 4-11 estrogen receptor 1 Rattus norvegicus 84-86 8031877-5 1994 The tritium-labeled diazirine [3H]-1 undergoes specific photocovalent attachment to ER with an attachment efficiency of 29% and a selectivity of 90%. Tritium 31-33 estrogen receptor 1 Rattus norvegicus 84-86 8031877-7 1994 SDS-polyacrylamide gel electrophoretic analysis of the photolabeled proteins shows specific labeling of a major species at M(r) 65,000, the same species that is labeled by [3H]tamoxifen aziridine, a well-characterized affinity label for ER. Tritium 173-175 estrogen receptor 1 Rattus norvegicus 237-239 8194735-6 1994 Treatment of granulosa and thecal cell cultures with IGF-I resulted in a dose-dependent increase in [3H]thymidine incorporation into DNA by both cell types. Tritium 101-103 insulin like growth factor 1 Gallus gallus 53-58 7889298-0 1994 Differential sensitivity of 3H-agonist binding to pre- and postsynaptic 5-HT1A receptors in bovine brain. Tritium 28-30 5-hydroxytryptamine receptor 1A Bos taurus 72-78 7984275-9 1994 The regional distribution of the 5-HT1F mRNA was determined in guinea-pig brain: high densities were observed in various cortical areas, the hippocampal formation and claustrum, which are regions known to contain 5-CT insensitive 5-HT1 or non 5-HT1A/1B/IC/ID [3H]5-HT binding sites. Tritium 260-262 5-hydroxytryptamine (serotonin) receptor 1F Mus musculus 33-39 3390445-4 1988 After treatment with endo-beta-galactosidase, 40-45% of the [3H]glucosamine or [3H]fucose radioactivity in the phospholipase-released glycoproteins was converted to products of disaccharide size, and the molecular size of the 158-kDa glycoprotein decreased to 145 kDa, demonstrating that it contains fucosylated poly-(N-acetyllactosaminyl) oligosaccharides. Tritium 80-82 galactosidase, beta 1 Rattus norvegicus 26-44 2458292-11 1988 Molecules, isolated from [3H]-fucose-labelled OTF9-63 cells by indirect immunoprecipitation with TEC-05 antibody, were degraded by extensive pronase digestion or mild alkaline treatment to large carbohydrate chains that were excluded from a Sephadex G-50 column. Tritium 26-28 POU domain, class 3, transcription factor 4 Mus musculus 46-50 7509793-11 1994 We show, in this study, that the intermediate filament protein vimentin is retinoylated in HL60 cells during a 24-h exposure to 100 nM [3H]RA. Tritium 136-138 vimentin Homo sapiens 63-71 8199879-1 1994 In human cortex and hippocampus area, [3H]5-HT (5 nM) labels 5-HT1A, 5-HT1D and 5-HT1E sites. Tritium 39-41 5-hydroxytryptamine receptor 1A Homo sapiens 61-67 7742265-6 1994 After exposure to insulin (10(-10) to 8 x 10(-6) M) or IGF-I (10(-10) to 10(-7) M) for 48 hours, VSMC incorporated [3H]thymidine to 200-280% of maximum (with insulin or IGF-I alone) compared to control. Tritium 116-118 insulin-like growth factor 1 Rattus norvegicus 55-60 7526860-8 1994 KGF stimulated 3H-thymidine incorporation in these end buds in the absence and presence of epidermal growth factor. Tritium 15-17 fibroblast growth factor 7 Mus musculus 0-3 3259723-4 1988 These supernatants, however, strongly suppressed IL-2-induced [3H]thymidine incorporation into the IL-2-sensitive cell line CTLL-2, whereas the urine preparations did not. Tritium 63-65 interleukin 2 Mus musculus 49-53 7957246-12 1994 The DAP-I-digested and native IGF-II species were also equipotent in stimulating [3H]thymidine incorporation into DNA in the human osteosarcoma cell line, MG-63. Tritium 82-84 insulin like growth factor 2 Homo sapiens 30-36 8203728-8 1994 Following digestion of 3H-NEM-labeled peptide by cathepsin G, the biotinylated side of the cleaved peptide is bound to the SPA bead, while the tritiated end of the cleaved peptide remains in solution. Tritium 23-25 cathepsin G Homo sapiens 49-60 2898111-7 1988 Cholecystokinin, angiotensin II, vasopressin, thyrotropin-releasing hormone and bombesin also stimulated [3H]IP1 production but were generally much weaker than BK. Tritium 106-108 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 109-112 8206647-3 1994 Epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) added singly caused significant increases relative to control in both the uptake of [3H]thymidine into the cellular DNA of subconfluent monolayers and of [3H]proline into collagenase-sensitive protein. Tritium 227-229 myotrophin Rattus norvegicus 10-23 8185808-6 1994 LHRH induced a 29% increase in the number of PRL mRNA-expressing cells processing through the S phase of the cell cycle, visualized by the incorporation of [3H]thymidine ([3H]T) into DNA over 16 h. The fraction of [3H]T-labelled cells was 10-12% of the total cell population. Tritium 157-159 gonadotropin releasing hormone 1 Rattus norvegicus 0-4 2837400-4 1988 We also describe here a non-GABA-associated [3H]SR 95531 binding site which is found at its highest level in the hippocampal CA1 region but also in CA2, CA3, dentate gyrus and cerebral cortex. Tritium 45-47 carbonic anhydrase 1 Rattus norvegicus 125-128 8185808-6 1994 LHRH induced a 29% increase in the number of PRL mRNA-expressing cells processing through the S phase of the cell cycle, visualized by the incorporation of [3H]thymidine ([3H]T) into DNA over 16 h. The fraction of [3H]T-labelled cells was 10-12% of the total cell population. Tritium 172-174 gonadotropin releasing hormone 1 Rattus norvegicus 0-4 8185808-6 1994 LHRH induced a 29% increase in the number of PRL mRNA-expressing cells processing through the S phase of the cell cycle, visualized by the incorporation of [3H]thymidine ([3H]T) into DNA over 16 h. The fraction of [3H]T-labelled cells was 10-12% of the total cell population. Tritium 172-174 gonadotropin releasing hormone 1 Rattus norvegicus 0-4 7858873-17 1994 We conclude that the desensitization of histamine H1-receptor-mediated [3H]-inositol phosphate formation occurs at the level of the receptor and involves a mechanism independent of activation of protein kinase A, G, or C, or calcium calmodulin-dependent protein kinase II. Tritium 72-74 histamine receptor H1 Homo sapiens 40-61 7956924-12 1994 The addition of 1 nM EGF to NFT-derived cells resulted in an increase in [3H]thymidine uptake to 237.5 +/- 19.8% (mean +/- SEM, n = 3) of the control value. Tritium 74-76 epidermal growth factor Homo sapiens 21-24 7927263-2 1994 The receptor was measured with a binding assay using a specific thromboxane A2 receptor antagonist, [3H]S-145. Tritium 101-103 thromboxane A2 receptor Rattus norvegicus 64-87 8122272-3 1994 PLA2 activity was measured by quantitating the release of [3H]arachidonic acid ([3H]AA) from rat pheochromocytoma (PC12) cells. Tritium 59-61 phospholipase A2 group IB Rattus norvegicus 0-4 8122272-7 1994 The PLA2 inhibitors dibucaine (50 microM) and mepacrine (50 microM) inhibited KCN-mediated [3H]AA release. Tritium 92-94 phospholipase A2 group IB Rattus norvegicus 4-8 8304976-7 1994 In addition, inhibition of [3H]thymidine incorporation after 2 hr directly paralleled inhibition of growth, suggesting an early effect at the level of DNA biosynthesis, possibly related to the down-regulation of c-myc expression. Tritium 28-30 MYC proto-oncogene, bHLH transcription factor Homo sapiens 212-217 7507320-4 1994 10(-10) M estradiol (E2) significantly stimulated MCF7 proliferation, and co-incubation of estrogen containing cultures with 50nM IGF-BP3 resulted in significant attenuation of the estrogen-stimulated proliferation ([3H]thymidine incorporation: E2, 147 +/- 18% of control; E2 +/- IGF-BP3, 111 +/- 18% of control, p < 0.05). Tritium 217-219 insulin like growth factor binding protein 3 Homo sapiens 130-137 7876332-4 1994 gp300 metabolically labeled with [3H]glucosamine and [35S]sulfate was chemically and enzymatically treated followed by Bio-Gel P-10 gel filtration. Tritium 34-36 deleted in malignant brain tumors 1 Mus musculus 0-5 2895019-4 1988 The competitive antagonist D-(-)-2-amino-5-phosphovaleric acid (AP-5) freezes the receptor in a state which precludes either binding of [3H]MK-801 to the receptor channel or its dissociation from it. Tritium 137-139 adaptor related protein complex 5 subunit beta 1 Homo sapiens 64-68 7876332-6 1994 Treatment of dual-labeled gp300 with PNGase F to cleave N-linked oligosaccharides released approximately 17% of [3H] and little [35S]. Tritium 113-115 deleted in malignant brain tumors 1 Mus musculus 26-31 7876332-6 1994 Treatment of dual-labeled gp300 with PNGase F to cleave N-linked oligosaccharides released approximately 17% of [3H] and little [35S]. Tritium 113-115 N-glycanase 1 Mus musculus 37-43 8280772-2 1994 They were, respectively, 5-, 300-, 500-, and 4000-fold weaker than PAF in each assay; inhibited PMN-binding of [3H]PAF at concentrations paralleling their biological potencies; and showed sensitivity to the inhibitory effects of PAF antagonists. Tritium 111-115 PCNA clamp associated factor Homo sapiens 67-70 7876334-4 1994 Cytosolic PLC activity was measured with [3H]PI and [3H]PIP2 as substrates. Tritium 42-44 heparan sulfate proteoglycan 2 Homo sapiens 10-13 3258238-1 1988 Cell-cycle progression of rat thymocytes stimulated with concanavalin A and interleukin 2 was monitored at 12-h intervals by pulse labeling aliquots of the cell culture with [3H]thymidine, by measuring cellular DNA and protein content and by counting the number of cells in the cultures. Tritium 175-177 interleukin 2 Rattus norvegicus 76-89 7876334-4 1994 Cytosolic PLC activity was measured with [3H]PI and [3H]PIP2 as substrates. Tritium 53-55 heparan sulfate proteoglycan 2 Homo sapiens 10-13 8187006-5 1994 Our experiments show that both GM-CSF and IL-3 can increase 3H-thymidine incorporation and cloning efficiency and reduce DNA synthesis time of H69 and, to a lesser extent, N417 cells, supporting the hypothesis that hematopoietic growth factors can stimulate the growth of some malignant nonhematopoietic cells in vitro. Tritium 60-62 colony stimulating factor 2 Homo sapiens 31-37 8187006-5 1994 Our experiments show that both GM-CSF and IL-3 can increase 3H-thymidine incorporation and cloning efficiency and reduce DNA synthesis time of H69 and, to a lesser extent, N417 cells, supporting the hypothesis that hematopoietic growth factors can stimulate the growth of some malignant nonhematopoietic cells in vitro. Tritium 60-62 interleukin 3 Homo sapiens 42-46 7931326-6 1994 PAF-induced inhibition of [3H]ACh release was blocked 90 +/- 3 and 86 +/- 2% by pertussis toxin and by anti-G alpha i1/2 antiserum incorporated into cortical synaptosomes, respectively. Tritium 27-29 G protein subunit alpha i2 Rattus norvegicus 108-120 2966310-1 1988 In human caudate and cortex membranes, [3H]serotonin ([3H]5-HT) labels 5-HT1A and 5-HT1C recognition sites which show nanomolar affinity for 8-OH-DPAT (8-hydroxy-2-(di-n-propylamino)-tetralin) and mesulergine respectively, whereas no 5-HT1B binding could be identified. Tritium 40-42 5-hydroxytryptamine receptor 1A Homo sapiens 71-77 7918619-6 1994 Chinese hamster ovary (CHO) cells transfected with a DNA fragment containing the coding regions of the human TRH receptor bound with [3H]TRH. Tritium 134-136 thyrotropin releasing hormone receptor Homo sapiens 109-121 7875535-4 1994 Antisense oligodeoxynucleotide at a concentration of 30 microM progressively reduced the specific binding [3H][Sar9, Met(O2)11]-SP, selective SP/NK1 receptor agonist, in the U-87 MG astrocytoma cells by approx. Tritium 107-109 tachykinin receptor 1 Homo sapiens 145-157 7531295-7 1994 In a [3H]labeled SP binding assay using mouse spinal cord membranes, [D-Trp7]sendide potently displaced [3H] labeled SP binding with a Ki value of 0.023 +/- 0.007 nM, which was approximately 140 and 9400 times more potent than that of unlabeled SP and CP-96,345, respectively. Tritium 105-107 tachykinin 1 Mus musculus 117-119 7531295-7 1994 In a [3H]labeled SP binding assay using mouse spinal cord membranes, [D-Trp7]sendide potently displaced [3H] labeled SP binding with a Ki value of 0.023 +/- 0.007 nM, which was approximately 140 and 9400 times more potent than that of unlabeled SP and CP-96,345, respectively. Tritium 105-107 tachykinin 1 Mus musculus 117-119 8118839-3 1994 Treatment of growth-arrested (in 0.4% fetal calf serum) cells with platelet-derived growth factor (0.3-7.5 nM) or angiotensin II (0.001-1000 nM) induced 3H-thymidine incorporation and cell proliferation in a dose-dependent manner (P < 0.01). Tritium 153-155 myotrophin Rattus norvegicus 84-97 7512304-10 1994 However, pretreatment of cultured hOB with IGF-II enhanced IGF-I-stimulated [3H]thymidine incorporation threefold. Tritium 77-79 insulin like growth factor 2 Homo sapiens 43-49 2966310-1 1988 In human caudate and cortex membranes, [3H]serotonin ([3H]5-HT) labels 5-HT1A and 5-HT1C recognition sites which show nanomolar affinity for 8-OH-DPAT (8-hydroxy-2-(di-n-propylamino)-tetralin) and mesulergine respectively, whereas no 5-HT1B binding could be identified. Tritium 55-57 5-hydroxytryptamine receptor 1A Homo sapiens 71-77 7834355-3 1994 The expression study revealed that 5HT1A mRNA distribution in the hippocampus and prefrontal cortex was consistent with the data of the [3H]8-OH-DPAT binding. Tritium 137-139 5-hydroxytryptamine receptor 1A Homo sapiens 35-40 3359327-2 1988 We now report that this transient AChE pattern is paralleled by a marked increase in [3H]nicotine binding sites in layer IV of area 17. Tritium 86-88 acetylcholinesterase Rattus norvegicus 34-38 7821974-5 1994 IL-2, IL-1 and IFN-gamma promoted [3H]thymidine incorporation in epitenon cells whereas TGF-beta had an inhibitory effect. Tritium 35-37 interleukin 2 Rattus norvegicus 0-4 7821974-5 1994 IL-2, IL-1 and IFN-gamma promoted [3H]thymidine incorporation in epitenon cells whereas TGF-beta had an inhibitory effect. Tritium 35-37 interferon gamma Rattus norvegicus 15-24 24222035-3 1994 For neurotensin, the most sensitive neuropeptide analyzed thus far in this work, the injection of 10 muL of a solution containing 320 zeptomolesy/gmL gave an [M + 3H](+3) ion at m/z 558.4 with S/N of > 8:1. Tritium 163-165 glycosylphosphatidylinositol anchored molecule like Homo sapiens 146-149 8196506-1 1994 [3H]Cytisine was evaluated as an in vivo ligand for the nicotinic cholinergic receptor (nAchR) in mouse brain. Tritium 1-3 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 88-93 8289485-2 1994 Granulocyte-macrophage colony-stimulating factor (GM-CSF) was given for 72 h before commencing chemotherapy in 15 patients with relapsed or refractory acute myeloid leukaemia (AML) and in 11 patients serial bone marrows were taken for measurement of [3H]thymidine labelling index, Ki-67 positivity and maximal binding of 5-(SAENTA-x8)-fluorescein, a flow cytometry ligand which enumerates nucleoside transporter sites. Tritium 251-253 colony stimulating factor 2 Homo sapiens 0-48 8171393-4 1994 Final LHRH cell division in Long-Evans rats was determined by [3H]thymidine autoradiography to take place over a short period between gestation days 12 and 13. Tritium 63-65 gonadotropin releasing hormone 1 Rattus norvegicus 6-10 8245983-5 1993 The rank order for substrate inhibition of [3H]5-HT uptake for both the previously reported rat vMAT1 and the human transporter clone followed the order 5-HT > dopamine > epinephrine > norepinephrine > 1-methyl-4-phenylpyridinium > 2-phenylethylamine > histamine. Tritium 44-46 solute carrier family 18 member A1 Rattus norvegicus 96-101 7932207-4 1994 In the presence of guanidinium phosphate, [3H]RX821002 bound with high affinity to a large and homogeneous population of sites in CC (Kd = 0.30 +/- 0.03 nM and Bmax = 271 +/- 7 fmol/mg of protein), SMG (Kd = 0.7 and Bmax = 274), human platelets (Kd = 0.6 nM and Bmx = 189) and neonatal rat lung (kd = 0.9 and Bmax = 161). Tritium 43-45 BMX non-receptor tyrosine kinase Homo sapiens 262-265 7893330-5 1994 A reduction in [3H]QNB binding was found in CA1 and CA3c 7 d after TMT, but not in CA3a, b, or the dentate gyrus. Tritium 16-18 carbonic anhydrase 1 Rattus norvegicus 44-47 7893330-7 1994 [3H]OXO-M binding decreased 1 d after exposure in CA1 and in all subfields of Ammon"s horn by d 3. Tritium 1-3 carbonic anhydrase 1 Rattus norvegicus 50-53 8241133-4 1993 Following purification and thrombin cleavage, a predominant monomeric (stokes radius = 2.3 nm, molecular mass of 32 kDa) [3H]retinoic acid hRAR alpha LBD complex was characterized by high-performance size-exclusion chromatography. Tritium 122-124 retinoic acid receptor alpha Homo sapiens 139-143 2836349-2 1988 In this paper, we have compared the relative gene copy number of dihydrofolate reductase (DHFR), the target enzyme of methotrexate (MTX), in order to clarify whether or not amplification of the gene is involved in the relative resistance to MTX observed for one of the cell lines, designated NaUCC-1, which is 4- to 5-fold more resistant to MTX as compared with the other cell lines and exhibits a reduced uptake of [3H]MTX. Tritium 417-419 dihydrofolate reductase Homo sapiens 90-94 8404644-9 1993 We were able to demonstrate aromatase activity by assaying the incorporation of tritium into [3H] water from [1 beta-3H]androstenedione. Tritium 80-87 aromatase Bos taurus 28-37 7845389-5 1994 Data from Western blot and two-dimensional gel electrophoresis of [3H] leucine and [32P] orthophosphate-labeled proteins showed the synthesis and phosphorylation of HSP28 in transfected cells after heating at 45 degrees C for 10 min. Tritium 67-69 heat shock protein family B (small) member 1 Homo sapiens 165-170 8404644-9 1993 We were able to demonstrate aromatase activity by assaying the incorporation of tritium into [3H] water from [1 beta-3H]androstenedione. Tritium 94-96 aromatase Bos taurus 28-37 2836823-9 1988 Similar results were found with 3H-NKA, 125I-NKA or 125I-BHNKA. Tritium 32-34 Natural killer alloreactivity QTL 1 Rattus norvegicus 35-38 7521512-2 1994 The selective NK1 receptor agonist [Sar9,Met(O2)11]-substance P stimulated a biphasic accumulation of [3H]inositol phosphates ([3H]IPs) in the presence of 10 mM LiCl in cells that had been prelabeled with [3H]inositol. Tritium 103-105 tachykinin receptor 1 Homo sapiens 14-26 7521512-5 1994 The selective PKC inhibitor Ro31-8220 did not prevent rapid NK1 receptor desensitization but after a longer incubation significantly potentiated human NK1 receptor agonist-stimulated accumulation of [3H]IPs. Tritium 200-202 tachykinin receptor 1 Homo sapiens 151-163 3128274-7 1988 The release of [3H]arachidonic acid metabolites appeared to be mediated by a transient increase in phospholipase A2 activity. Tritium 16-18 LOC104974671 Bos taurus 99-115 7522266-9 1994 Moreover, fetal growth and the transfer of 3H-AIB to fetus are accelerated when IGFBP-1 is neutralized by polyclonal antibody, suggesting that maternal IGFBP-1 inhibits fetal growth by inhibiting IGF-I action on the placenta. Tritium 43-45 insulin like growth factor binding protein 1 Homo sapiens 80-87 7522266-9 1994 Moreover, fetal growth and the transfer of 3H-AIB to fetus are accelerated when IGFBP-1 is neutralized by polyclonal antibody, suggesting that maternal IGFBP-1 inhibits fetal growth by inhibiting IGF-I action on the placenta. Tritium 43-45 insulin like growth factor binding protein 1 Homo sapiens 152-159 8288318-5 1993 Exogenous IL-2 afforded a degree of restoration of both IL-2 receptor expression and [3H]thymidine uptake which was substantial at the lower, but very small--if any--at the higher, parasite concentrations tested. Tritium 86-88 interleukin 2 Mus musculus 10-14 8106194-1 1993 Tritium-labeled growth hormone releasing peptide His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 was synthesized by tritium-halogen exchange on the precursor His-5,7-Br2-D-Trp-Ala-Trp-D-Phe-Lys-NH2. Tritium 0-7 ghrelin and obestatin prepropeptide Homo sapiens 16-48 8106194-1 1993 Tritium-labeled growth hormone releasing peptide His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 was synthesized by tritium-halogen exchange on the precursor His-5,7-Br2-D-Trp-Ala-Trp-D-Phe-Lys-NH2. Tritium 100-107 ghrelin and obestatin prepropeptide Homo sapiens 16-48 2827687-1 1988 The effects of fentanyl isothiocyanate (FIT) and pertussis toxin on the binding of [3H]D-Ala2, D-Leu5-enkephalin ([3H]DADLE) to rat brain membranes were compared. Tritium 84-86 proenkephalin Rattus norvegicus 102-112 8287562-0 1993 A fibroblast glutaryl-CoA dehydrogenase assay using detritiation of 3H-labelled glutaryl-CoA: application in the genotyping of the glutaryl-CoA dehydrogenase locus. Tritium 68-70 glutaryl-CoA dehydrogenase Homo sapiens 13-39 8287562-0 1993 A fibroblast glutaryl-CoA dehydrogenase assay using detritiation of 3H-labelled glutaryl-CoA: application in the genotyping of the glutaryl-CoA dehydrogenase locus. Tritium 68-70 glutaryl-CoA dehydrogenase Homo sapiens 131-157 8025267-4 1994 This form of CD44 also had the capacity to bind hyaluronan, because detergent extracts of platelets as well as intact platelets could bind soluble [3H]hyaluronan, and this property was blocked by antibodies directed against CD44. Tritium 148-150 CD44 molecule (Indian blood group) Rattus norvegicus 13-17 8025267-4 1994 This form of CD44 also had the capacity to bind hyaluronan, because detergent extracts of platelets as well as intact platelets could bind soluble [3H]hyaluronan, and this property was blocked by antibodies directed against CD44. Tritium 148-150 CD44 molecule (Indian blood group) Rattus norvegicus 224-228 8034615-9 1994 By Scatchard analysis, the Kd values for [3H]9-cis-retinoic acid to the GST-hRXR alpha (DEF) peptide and the cleaved DEF peptide were estimated to be 1.8 nM and 5.6 nM, respectively. Tritium 42-44 glutathione S-transferase kappa 1 Homo sapiens 72-75 2827687-1 1988 The effects of fentanyl isothiocyanate (FIT) and pertussis toxin on the binding of [3H]D-Ala2, D-Leu5-enkephalin ([3H]DADLE) to rat brain membranes were compared. Tritium 115-117 proenkephalin Rattus norvegicus 102-112 2833227-3 1988 Similarly when PAF was added to [3H]-glycerol prelabelled macrophages there was a rapid increase in 1,2-diacyl[3H]glycerol levels. Tritium 33-35 PCNA clamp associated factor Homo sapiens 15-18 7801776-4 1994 IC50 and K1 values of l-norepinephrine (NE), dl-propranol (Pro), d-timolol (d-Tim), and l-Tim competed for the [3H]DHA binding sites were 171 +/- 10, 10.3 +/- 4.3, 6.5 +/- 2.1, 0.40 +/- 0.23 nmol.L-1 and 113 +/- 6, 6.3 +/- 2.8, 4.0 +/- 1.3, 0.25 +/- 0.14 nmol.L-1, respectively. Tritium 112-114 L1 cell adhesion molecule Mus musculus 196-218 7801776-7 1994 The results suggested that these binding sites for [3H]DHA in mouse myometrium appeared to be beta 2-adrenoceptor. Tritium 52-54 adrenergic receptor, beta 2 Mus musculus 94-113 8206972-5 1994 Addition of LPL (10 micrograms/ml) to BFC-1 beta adipocytes produced a 2-fold increase in cellular uptake of [3H]retinoid from a lipid emulsion containing [3H]retinyl ester. Tritium 110-112 lipoprotein lipase Oryctolagus cuniculus 12-15 8206972-5 1994 Addition of LPL (10 micrograms/ml) to BFC-1 beta adipocytes produced a 2-fold increase in cellular uptake of [3H]retinoid from a lipid emulsion containing [3H]retinyl ester. Tritium 156-158 lipoprotein lipase Oryctolagus cuniculus 12-15 7902194-6 1993 However, in contrast to the placebo group, NMDA-sensitive [3H]glutamate binding in strata radiatum and oriens of the CA1 area was not significantly reduced in the CGP 39551 group. Tritium 59-61 carbonic anhydrase 1 Rattus norvegicus 117-120 8398696-9 1993 Inhibition of TS in intact cells was determined by measuring 3H-UdR incorporation in two cell lines. Tritium 61-63 thymidylate synthetase Homo sapiens 14-16 7691407-5 1993 By Western blot analysis and immunoprecipitation studies using the Raji Burkitt"s lymphoma cell line metabolically labelled with [3H]leucine, the LL2 antigen was determined to correspond to a molecular mass of 140 kDa. Tritium 130-132 peroxiredoxin 2, pseudogene 1 Mus musculus 146-149 2833227-5 1988 Increasing concentrations of PAF led to a markedly similar increase in both ROI production and [3H]inositol lipid hydrolysis suggesting that inositol lipid hydrolysis may lead to the generation of ROI in macrophages. Tritium 96-98 PCNA clamp associated factor Homo sapiens 29-32 3260131-5 1988 The immunotoxin retained both RIP and antibody activity, inhibiting protein synthesis both in a cell-free system and in cells bearing the Campath 1 antigen; it also abolished methyl 3H-thymidine uptake in phytohemagglutinin-stimulated T lymphocytes. Tritium 182-184 regulation of phenobarbitol-inducible P450 Mus musculus 30-33 8376986-3 1993 IL-2 (10(-14)-10(-8) M) modulated K(+)-evoked [3H]DA release in a biphasic manner, with low concentrations (10(-12)-10(-11) M) of IL-2 potentiating and higher concentrations (10(-9)-10(-8) M) inhibiting K(+)-induced [3H]DA release. Tritium 47-49 interleukin 2 Rattus norvegicus 0-4 8376986-3 1993 IL-2 (10(-14)-10(-8) M) modulated K(+)-evoked [3H]DA release in a biphasic manner, with low concentrations (10(-12)-10(-11) M) of IL-2 potentiating and higher concentrations (10(-9)-10(-8) M) inhibiting K(+)-induced [3H]DA release. Tritium 217-219 interleukin 2 Rattus norvegicus 0-4 8026463-3 1994 Two very polar [3H]mannose-labeled glycolipids named CP1 and CP2 qualified as GPI precursor lipids since their carbohydrate head group, Man alpha 1,2(X-->PO4-->6)Man alpha 1,2Man alpha 1,6Man alpha-GlcN-inositol (with X most likely being ethanolamine) comprises the core structure which is common to all GPI anchors described so far. Tritium 16-18 ceruloplasmin Homo sapiens 61-64 2831066-7 1988 mIL4 produced by the similar transformants induced [3H]thymidine uptake of a T cell line, a mast cell line and B leukemia cells, and enhanced the production of IgG1 by B cells. Tritium 52-54 interleukin 4 Mus musculus 0-4 7526648-4 1994 Since IL-2 enhanced [3H]-leucine uptake into mast cells, this suggests that protein synthesis may be related in some way with the inhibition of histamine release. Tritium 21-23 interleukin 2 Rattus norvegicus 6-10 8376986-5 1993 The inhibition by IL-2 of K(+)-evoked [3H]DA release was reversible and not due to neurotoxicity, as preexposure to IL-2 (10(-8) M) had no significant effect on the subsequent ability of dopaminergic cells to take up and to release [3H]DA. Tritium 39-41 interleukin 2 Rattus norvegicus 18-22 8376986-5 1993 The inhibition by IL-2 of K(+)-evoked [3H]DA release was reversible and not due to neurotoxicity, as preexposure to IL-2 (10(-8) M) had no significant effect on the subsequent ability of dopaminergic cells to take up and to release [3H]DA. Tritium 233-235 interleukin 2 Rattus norvegicus 18-22 8376986-7 1993 The results of this study indicate that IL-2 is able to potentiate [3H]DA release evoked by a number of different stimuli, including K+ depolarization and activation of both NMDA and non-NMDA receptor subtypes in mesencephalic cell cultures. Tritium 68-70 interleukin 2 Rattus norvegicus 40-44 8114468-12 1993 If IL-1 is added to the incubation medium, which contains [3H]-labeled arachidonic acid, PG-E2 synthesis increases more than in normal corneal cells. Tritium 59-61 interleukin 1 alpha Homo sapiens 3-7 8188767-11 1994 The anti-IGF-I receptor antibody alpha IR3 blocked the IGF-I and IGF-II stimulated increase of 3H-thymidine uptake. Tritium 95-97 insulin like growth factor 2 Homo sapiens 65-71 2828802-2 1988 Binding saturation experiments were performed in rat brain crude membrane preparations with the opioid mu-selective ligand [3H]-[D-Ala2,MePhe4,Gly-ol5]enkephalin (DAGO), using a sequential procedure. Tritium 124-126 proenkephalin Rattus norvegicus 151-161 8180235-1 1994 In order to study the function of the brown-fat specific uncoupling protein thermogenin (UCP), the effect of certain sulfonates on [3H]GDP binding to the GDP-binding site of brown adipose tissue mitochondria was studied. Tritium 132-134 uncoupling protein 1 Homo sapiens 76-87 7694524-4 1993 In these bioassays the changes in optical density induced by IL-2 and IL-6 measured with the neutral red assay are directly comparable with the relative changes measured with the [3H]thymidine incorporation assay. Tritium 180-182 interleukin 2 Mus musculus 61-65 2835700-5 1988 Additional experiments demonstrated that [3H]FOXY can be used as a high yield photoaffinity label for the mu opiate receptor subtype. Tritium 42-44 opioid receptor mu 1 Homo sapiens 106-124 7690567-1 1993 Interleukin 3-dependent BNu-2cl3 mast cells, mucosal type-like mast cells, exhibited a specific high-affinity binding site for [3H]prostaglandin (PG) E2. Tritium 128-130 interleukin 3 Homo sapiens 0-13 7693284-2 1993 We investigated the effects of the non-peptide NK1 receptor antagonist, CP-96,345, its inactive enantiomer CP-96,344, and the racemic mixture (+/-)-CP-96,345, on the binding of [3H]-nimodipine and [3H]-diltiazem to L-type calcium channels in rat cerebral cortex membranes. Tritium 178-180 tachykinin receptor 1 Rattus norvegicus 47-59 8207583-7 1994 Since insulin-like growth factor-I increased [3H]thymidine incorporation after 48 hours (50% increase) but not after 24 hours of exposure, these observations represent a synergistic interaction. Tritium 46-48 insulin like growth factor 1 Gallus gallus 6-34 8149478-2 1994 This study showed that there was a highly selective solvent-resistant binding of radioactive substance in endothelial cells of heart and kidney 1 day following injection of [3H]-Trp-P-1 (0.1 or 1.5 mg/kg) in NMRI mice treated with the P450-inducing agent beta-naphthoflavone (BNF). Tritium 174-176 natriuretic peptide type B Mus musculus 235-280 8149478-6 1994 On incubation of heart and kidney slices with [3H]-Trp-P-1, there was a binding of radioactivity in endothelial cells of BNF-treated mice, but not in corn oil-treated mice. Tritium 47-49 natriuretic peptide type B Mus musculus 121-124 8149478-8 1994 The results indicate an in situ metabolism of [3H]-Trp-P-1 to a reactive species, catalysed by a BNF-inducible enzyme, possibly P4501A1, in endothelial cells in the heart and kidney. Tritium 47-49 natriuretic peptide type B Mus musculus 97-100 8143769-4 1994 Stimulation of [3H]thymidine and [35S]sulfate incorporation by IGF-I was two to four times that in serum-free controls, with half-maximal stimulation at 1 x 10(-9) M. The efficacy of IGF-I was approximately one-half of that of serum in stimulating [3H]thymidine incorporation and was comparable to that of serum for [35S]sulfate incorporation. Tritium 16-18 insulin-like growth factor 1 Rattus norvegicus 63-68 8143769-4 1994 Stimulation of [3H]thymidine and [35S]sulfate incorporation by IGF-I was two to four times that in serum-free controls, with half-maximal stimulation at 1 x 10(-9) M. The efficacy of IGF-I was approximately one-half of that of serum in stimulating [3H]thymidine incorporation and was comparable to that of serum for [35S]sulfate incorporation. Tritium 249-251 insulin-like growth factor 1 Rattus norvegicus 63-68 8143769-11 1994 When chondrosarcoma chondrocytes were incubated with fetal calf serum, anti-IGF-I antibody exerted a minimal inhibitory effect, reducing both [3H]thymidine and [35S]sulfate incorporation by less than 25%. Tritium 143-145 insulin-like growth factor 1 Rattus norvegicus 76-81 7693284-17 1993 The results indicate that in addition to possessing affinity for the NK1 receptor, the non-peptide antagonist, CP-96,345, displays high affinity for [3H]-diltiazem binding sites on L-type calcium channels.The functional effect that may be observed in integrated models will be a consequence of either property, or be a composite effect of NK1 receptor antagonism and L-channel blockade. Tritium 150-152 tachykinin receptor 1 Rattus norvegicus 339-351 8247264-2 1993 Light-microscopic examination revealed a negligible number of [3H]thymidine-labeled cells showing neuron-specific enolase immunoreactivity during mitosis. Tritium 63-65 enolase 2 Rattus norvegicus 98-121 8247264-3 1993 However, by two weeks after [3H]thymidine administration, a significant increase in the density of [3H]thymidine-labeled neuron-specific enolase-immunoreactive cells was detected. Tritium 29-31 enolase 2 Rattus norvegicus 121-144 8247264-3 1993 However, by two weeks after [3H]thymidine administration, a significant increase in the density of [3H]thymidine-labeled neuron-specific enolase-immunoreactive cells was detected. Tritium 100-102 enolase 2 Rattus norvegicus 121-144 8247264-5 1993 At the four-week time-point, [3H]thymidine-labeled neuron-specific enolase-immunoreactive cells were indistinguishable from neighboring granule cells. Tritium 30-32 enolase 2 Rattus norvegicus 51-74 8014598-4 1994 LHRH induced the release of [3H]arachidonic acid ([3H]AA) from [3H]AA-prelabelled anterior pituitary tissue from pro-oestrous rats; a response which was sensitive to inhibitors of PLA2, of protein kinase C (PKC) and of protein synthesis. Tritium 29-31 gonadotropin releasing hormone 1 Rattus norvegicus 0-4 8014598-4 1994 LHRH induced the release of [3H]arachidonic acid ([3H]AA) from [3H]AA-prelabelled anterior pituitary tissue from pro-oestrous rats; a response which was sensitive to inhibitors of PLA2, of protein kinase C (PKC) and of protein synthesis. Tritium 29-31 phospholipase A2 group IB Rattus norvegicus 180-184 8014598-4 1994 LHRH induced the release of [3H]arachidonic acid ([3H]AA) from [3H]AA-prelabelled anterior pituitary tissue from pro-oestrous rats; a response which was sensitive to inhibitors of PLA2, of protein kinase C (PKC) and of protein synthesis. Tritium 51-53 gonadotropin releasing hormone 1 Rattus norvegicus 0-4 3283711-1 1988 Using 3H-labeled luteinizing hormone-releasing hormone (LHRH) at low concentrations, the in vitro proteolytic inactivation of the peptide hormone by whole rat ovaries was studied and compared with that by the soluble and particulate rat ovarian fraction. Tritium 6-8 gonadotropin releasing hormone 1 Rattus norvegicus 17-54 8014598-4 1994 LHRH induced the release of [3H]arachidonic acid ([3H]AA) from [3H]AA-prelabelled anterior pituitary tissue from pro-oestrous rats; a response which was sensitive to inhibitors of PLA2, of protein kinase C (PKC) and of protein synthesis. Tritium 51-53 phospholipase A2 group IB Rattus norvegicus 180-184 8014598-5 1994 Activation of PKC also resulted in [3H]AA release which was inhibited with exactly the same pharmacological profile as the response to LHRH. Tritium 36-38 gonadotropin releasing hormone 1 Rattus norvegicus 135-139 8349705-6 1993 Moreover, CCK-8 and gastrin I dose-dependently increased [3H]thymidine incorporation of the CHO transfectants in serum-free medium and promoted cell growth. Tritium 58-60 gastrin Homo sapiens 20-27 3131834-2 1988 Recombinant interleukin-1 induced an approximate 3-fold increase in [3H]-AA release, a 7-fold increase in PGE2 production and a 2-fold increase in PLA2 activity in human synovial fibroblasts. Tritium 69-71 interleukin 1 alpha Homo sapiens 12-25 8402586-4 1993 Then the cells were exposed to increasing levels of cold and 3H-labelled B(A)P for varying times. Tritium 61-63 prohibitin 2 Homo sapiens 73-78 8335925-7 1993 The levels of MCP-1 and IL-8 protein from mixed lymphocyte reaction supernatants as measured by specific ELISA were positively correlated with the proliferative response as measured by [3H]TdR uptake. Tritium 186-188 C-C motif chemokine ligand 2 Homo sapiens 14-19 8014598-6 1994 Both gonadotrophin secretion and [3H]AA release responses to LHRH and to phorbol ester varied in parallel during the oestrous cycle and in ovariectomized/oestradiol-17 beta-replaced animals, as did their sensitivity to quinacrine and the protein synthesis inhibitor cycloheximide. Tritium 34-36 gonadotropin releasing hormone 1 Rattus norvegicus 61-65 3322402-1 1987 The in vitro binding of [3H]SR42128 (Iva-Phe-Nle-Sta-Ala-Sta-Arg), a potent inhibitor of human renin activity, to purified human renin and a number of other aspartic proteases was examined. Tritium 25-27 GCY Homo sapiens 49-52 8061121-5 1994 LAK effector cells from TNF-alpha plus IL-2 cultures responded with an increased [3H]thymidine uptake to restimulation by IL-2 alone. Tritium 82-84 interleukin 2 Mus musculus 39-43 8061121-5 1994 LAK effector cells from TNF-alpha plus IL-2 cultures responded with an increased [3H]thymidine uptake to restimulation by IL-2 alone. Tritium 82-84 interleukin 2 Mus musculus 122-126 3322402-1 1987 The in vitro binding of [3H]SR42128 (Iva-Phe-Nle-Sta-Ala-Sta-Arg), a potent inhibitor of human renin activity, to purified human renin and a number of other aspartic proteases was examined. Tritium 25-27 GCY Homo sapiens 57-60 8346241-5 1993 Immunoprecipitation of [3H]myristic acid-radiolabeled human monocyte lysates with IgG antibodies to the 31-kDa IL-1 alpha precursor recovered a protein with the physicochemical properties of the IL-1 alpha N-terminal propiece (16 kDa, pI 4.45). Tritium 24-26 interleukin 1 alpha Homo sapiens 111-121 3500080-11 1987 Autoradiographic analysis with [3H]PRO indicated that EGF treatment caused significant increases in grain counts per unit area in ameloblast, odontoblast, and PDL regions studied. Tritium 32-34 epidermal growth factor Mus musculus 54-57 8319182-3 1993 The binding of (125-I) EGF to these cell lines and the stimulatory effect of EGF on (3H) thymidine incorporation into DNA were examined. Tritium 85-87 epidermal growth factor Homo sapiens 77-80 8004455-0 1994 Changes in [3H]PAF binding and PAF concentrations in gerbil brain after bilateral common carotid artery occlusion: a quantitative autoradiographic study. Tritium 12-14 PCNA clamp associated factor Homo sapiens 15-18 8004455-2 1994 In sagittal sections of gerbil brain, labeling with [3H]PAF was mainly located in the cortex, hippocampus and cerebellum. Tritium 53-55 PCNA clamp associated factor Homo sapiens 56-59 8004455-8 1994 These results indicate that PAF may be involved in the early stages of brain ischemia in the gerbil and suggest that endogenous PAF produced during ischemia may contribute to the down-regulation of [3H]PAF binding sites in the brain. Tritium 199-201 PCNA clamp associated factor Homo sapiens 28-31 8004455-8 1994 These results indicate that PAF may be involved in the early stages of brain ischemia in the gerbil and suggest that endogenous PAF produced during ischemia may contribute to the down-regulation of [3H]PAF binding sites in the brain. Tritium 199-201 PCNA clamp associated factor Homo sapiens 128-131 8004455-8 1994 These results indicate that PAF may be involved in the early stages of brain ischemia in the gerbil and suggest that endogenous PAF produced during ischemia may contribute to the down-regulation of [3H]PAF binding sites in the brain. Tritium 199-201 PCNA clamp associated factor Homo sapiens 128-131 8160783-11 1994 The enhanced functional recovery with EPO administration was accompanied by an increased tubular regeneration and [3H]thymidine incorporation in the cortical tissue. Tritium 115-117 erythropoietin Rattus norvegicus 38-41 8319182-9 1993 EGF increased 3H-thymidine incorporation in all three cell lines. Tritium 14-16 epidermal growth factor Homo sapiens 0-3 8104885-3 1993 These CD4+ T cells were T-cell receptor (TcR)/CD3+ and the cross-linking of either TcR alone or both TcR and CD4 molecules induced [3H]thymidine deoxyribose (TdR) incorporation, similar to functional CD4+ T cells. Tritium 132-135 T cell receptor alpha variable 12-3 Rattus norvegicus 24-39 8104885-3 1993 These CD4+ T cells were T-cell receptor (TcR)/CD3+ and the cross-linking of either TcR alone or both TcR and CD4 molecules induced [3H]thymidine deoxyribose (TdR) incorporation, similar to functional CD4+ T cells. Tritium 132-135 T cell receptor alpha variable 12-3 Rattus norvegicus 41-44 8104885-3 1993 These CD4+ T cells were T-cell receptor (TcR)/CD3+ and the cross-linking of either TcR alone or both TcR and CD4 molecules induced [3H]thymidine deoxyribose (TdR) incorporation, similar to functional CD4+ T cells. Tritium 132-135 T cell receptor alpha variable 12-3 Rattus norvegicus 83-86 8104885-3 1993 These CD4+ T cells were T-cell receptor (TcR)/CD3+ and the cross-linking of either TcR alone or both TcR and CD4 molecules induced [3H]thymidine deoxyribose (TdR) incorporation, similar to functional CD4+ T cells. Tritium 132-135 T cell receptor alpha variable 12-3 Rattus norvegicus 83-86 7523053-4 1994 100ng/ml of SCF caused substantial reduction in colony number and 3H-TdR uptake although this suppression was of lower magnitude than those induced by IL3, IL6 or GM-CSF. Tritium 66-68 KIT ligand Rattus norvegicus 12-15 3120599-3 1987 In the absence of Na+, the dicarboxylates glutarate and suberate cis-inhibited and trans-stimulated [3H]PAH uptake, indicating a common transport system. Tritium 101-103 phenylalanine hydroxylase Rattus norvegicus 104-107 8106442-6 1994 However, using ionophore-permeabilized monocytes and controlled intracellular Ca2+ concentration it was possible to dissociate MCP-1-induced Ca2+ influx from [3H]arachidonic acid release. Tritium 159-161 C-C motif chemokine ligand 2 Homo sapiens 127-132 8391763-2 1993 The tritiated sodium channel blocker saxitoxin ([3H]-saxitoxin; STX) was used to detect toxins in paralytic shellfish poisoning (PSP) by measuring the competitive displacement of other toxins. Tritium 48-51 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 Rattus norvegicus 64-67 8391763-3 1993 With this assay the amount of STX and tetrodotoxin needed to displace 50% of bound [3H]STX was 1.7 and 1.76 ng/ml for buffer samples, respectively. Tritium 84-86 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 Rattus norvegicus 30-33 8391763-3 1993 With this assay the amount of STX and tetrodotoxin needed to displace 50% of bound [3H]STX was 1.7 and 1.76 ng/ml for buffer samples, respectively. Tritium 84-86 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 Rattus norvegicus 87-90 7508865-0 1994 Phospholipase A2 enhances [3H]AMPA binding to a putative homomeric GluR-B receptor in the rat spinal cord. Tritium 27-29 phospholipase A2 group IB Rattus norvegicus 0-16 3120599-4 1987 In the presence of Na+, 10 microM glutarate in the incubation medium did not cis-inhibit, but rather stimulated [3H]PAH uptake and caused PAH accumulation above equilibrium distribution ("overshoot"). Tritium 113-115 phenylalanine hydroxylase Rattus norvegicus 116-119 7508865-2 1994 Phospholipase A2 elicited a dose-dependent increase in specific [3H]AMPA binding but not in [3H]kainate binding. Tritium 65-67 phospholipase A2 group IB Rattus norvegicus 0-16 7508865-4 1994 Since GluR-B is the only AMPA receptor subunit detectable in the rat spinal cord, and recombinant homomeric GluR-B assemblies do not bind [3H]kainate, these results suggest phospholipase A2 may modulate [3H]AMPA binding to putative homomeric GluR-B receptors. Tritium 204-206 phospholipase A2 group IB Rattus norvegicus 173-189 3693230-4 1987 Concomitantly, there was a significant decrease in the transpulmonary metabolism of [3H]BPAP and the calculated apparent first-order reaction constant Amax/Km of ACE for [3H]BPAP. Tritium 85-87 angiotensin-converting enzyme Oryctolagus cuniculus 162-165 8306341-6 1994 Prelabeling cells with [3H]glycerol or [3H]-choline demonstrated rapid release of [3H]phosphorylcholine and a decrease in [3H]glycerol-labeled phosphatidylcholine in response to IL-3 stimulation. Tritium 24-26 interleukin 3 Homo sapiens 178-182 7683979-9 1993 IGFBP-3 induced a dose-dependent inhibition of protein synthesis stimulated by IGF-I; IGFBP-3 (10(-7) M) completely inhibited the [3H]leucine uptake stimulated by IGF-I (10(-8) M). Tritium 131-133 insulin-like growth factor binding protein 3 Rattus norvegicus 0-7 7683979-9 1993 IGFBP-3 induced a dose-dependent inhibition of protein synthesis stimulated by IGF-I; IGFBP-3 (10(-7) M) completely inhibited the [3H]leucine uptake stimulated by IGF-I (10(-8) M). Tritium 131-133 insulin-like growth factor binding protein 3 Rattus norvegicus 86-93 7683979-9 1993 IGFBP-3 induced a dose-dependent inhibition of protein synthesis stimulated by IGF-I; IGFBP-3 (10(-7) M) completely inhibited the [3H]leucine uptake stimulated by IGF-I (10(-8) M). Tritium 131-133 insulin-like growth factor 1 Rattus norvegicus 163-168 8365828-6 1993 suppressed anti-CD3-induced proliferation, IL-2-driven 3H-TdR incorporation, and IL-2R expression. Tritium 55-57 interleukin 2 Mus musculus 43-47 3693230-4 1987 Concomitantly, there was a significant decrease in the transpulmonary metabolism of [3H]BPAP and the calculated apparent first-order reaction constant Amax/Km of ACE for [3H]BPAP. Tritium 171-173 angiotensin-converting enzyme Oryctolagus cuniculus 162-165 8365828-9 1993 LNC from TCDD-treated mice also had enhanced 3H-TdR incorporation when cultured in the presence of a saturating amount of exogenous mIL-2. Tritium 45-47 interleukin 2 Mus musculus 132-137 7912839-3 1994 Whereas the binding characteristics of various agonist and antagonist ligands to the N-methyl-D-aspartate and the AMPA receptors were not modified by trimipramine treatment, there was a significant reduction in the increase in 3H-AMPA binding elicited by PLA2 treatment. Tritium 227-229 phospholipase A2 group IB Rattus norvegicus 255-259 3693230-7 1987 To resolve this, we studied the effect of PMA on the Km (a measure of enzyme affinity for its substrate) and Amax (a derivative of Vmax that is dependent upon total enzyme present and thus capillary surface area) of ACE and 5"-nucleotidase for [3H]BPAP and [14C]AMP, respectively. Tritium 245-247 angiotensin-converting enzyme Oryctolagus cuniculus 216-219 2856403-7 1987 Insulin/IGF-I plus TSH or insulin/IGF-I plus norepinephrine can increase both proto-oncogene expression and [3H]thymidine incorporation into DNA to the same extent; however, the former combination can increase cell number whereas the latter cannot. Tritium 109-111 insulin-like growth factor 1 Rattus norvegicus 8-13 8300636-4 1994 In this study, sensitivity to bacterial phosphatidylinositol-specific phospholipase C, biosynthetic labeling with [3H]ethanolamine, and partitioning in Triton X-114 are used to establish that p97 is expressed at the cell surface as a glycosylphosphatidylinositol-anchored protein. Tritium 115-117 melanotransferrin Homo sapiens 192-195 8157071-4 1994 Saturation analyses of [3H]kainic acid binding demonstrated that GluR6 expressed in Sf9 cell membranes bound [3H]kainic acid at a single high affinity site with a dissociation constant of 12 nM. Tritium 24-26 glutamate ionotropic receptor kainate type subunit 2 Homo sapiens 65-70 7907983-2 1994 [3H]Glucosamine incorporation was stimulated in the corpus region by the histamine H2 receptor antagonists which have a six-membered aromatic ring, roxatidine and FRG-8813, and the new H+,K(+)-ATPase inhibitor, NC-1300-O-3. Tritium 1-3 histamine receptor H 2 Rattus norvegicus 73-94 8011537-2 1994 IL-1 alpha, IL-1 beta and TNF-alpha stimulated 3H-TdR uptake in leukaemic blasts in a dose-dependent fashion. Tritium 47-49 interleukin 1 alpha Homo sapiens 0-10 8275974-8 1994 These studies also suggest that enhancement of [3H]nicotine binding by activated protein kinase A may not involve synthesis of new receptor subunit proteins. Tritium 48-50 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 81-97 8319831-8 1993 However, C terminal-specific gastrin antibodies, even at low concentration, caused a dramatic decrease in both cell number (IC50 = 1:4000 antiserum dilution) and [3H]thymidine incorporation (IC50 = 1:400 antiserum dilution) in the HGT-1 cell line. Tritium 163-165 gastrin Homo sapiens 29-36 8473310-4 1993 Characterization of the two acylation events revealed that, in contrast to the p56lck-associated radioactivity from [3H]myristate-labeled cells, the p56lck-associated radioactivity from [3H]palmitate-labeled cells was susceptible to cleavage by neutral hydroxylamine and was not blocked by inhibitors of protein synthesis. Tritium 187-189 LCK proto-oncogene, Src family tyrosine kinase Homo sapiens 149-155 8473310-5 1993 Pulse-chase analyses revealed that the labeling of p56lck with [3H]palmitate, but not [3H]myristate, was reversible. Tritium 64-66 LCK proto-oncogene, Src family tyrosine kinase Homo sapiens 51-57 8473310-6 1993 The presence of covalently attached palmitate on p56lck from [3H]palmitate-labeled cells was verified by thin-layer chromatography following acid hydrolysis of the acylated protein. Tritium 62-64 LCK proto-oncogene, Src family tyrosine kinase Homo sapiens 49-55 8473310-7 1993 2-Hydroxymyristate, which is metabolically activated to form a potent inhibitor of protein myristoylation, specifically inhibited the acylation of p56lck with [3H]myristate without affecting its labeling with [3H]palmitate. Tritium 160-162 LCK proto-oncogene, Src family tyrosine kinase Homo sapiens 147-153 8385131-3 1993 This study demonstrates that the alpha 2AAR incorporates [3H] palmitic acid in metabolic labeling studies and that mutation of Cys-442 to Ala or Ser eliminates detectable 3H-palmitoylation. Tritium 58-60 adrenoceptor alpha 2A Canis lupus familiaris 33-43 8385131-11 1993 Metabolic labeling of Madin-Darby canine kidney cells expressing wild-type (Cys-442) or mutant (Ala-442) alpha 2AARs with [3H]palmitic acid indicated that only wild-type Cys-442-containing receptors incorporated [3H]palmitate, monitored following isolation of the alpha 2AAR detergent extracts using yohimbine-agarose chromatography. Tritium 123-125 adrenoceptor alpha 2A Canis lupus familiaris 105-115 8491253-5 1993 In contrast, [3H]5-HT binding determined in the presence of drugs masking 5-HT1A, 5-HT1B and 5-HT1C receptors, is markedly (50%) reduced 3 days after the viral infection. Tritium 14-16 5-hydroxytryptamine receptor 1A Homo sapiens 74-80 8395280-2 1993 The expressed recombinant RARs (rRARs: rRAR alpha/E, rRAR beta/E and rRAR gamma) showed nearly the same magnitude of binding affinity toward [3H]retinoic acid (RA) as hRARs extracted from human cells (Ka values: 6.0 x 10(9) M-1 for rRAR alpha/E and 2.7 x 10(10) M-1 for both rRAR beta/E and rRAR gamma). Tritium 142-144 retinoic acid receptor, alpha Rattus norvegicus 39-49 8263509-5 1994 Phospholipases A2 and C and p-chloromercuribenzosulfonic acid were invariably effective in significantly inhibiting [3H]DCKA binding with [3H]Gly binding being unaltered. Tritium 117-119 phospholipase A2 group IB Rattus norvegicus 0-23 8263509-5 1994 Phospholipases A2 and C and p-chloromercuribenzosulfonic acid were invariably effective in significantly inhibiting [3H]DCKA binding with [3H]Gly binding being unaltered. Tritium 139-141 phospholipase A2 group IB Rattus norvegicus 0-23 2856403-7 1987 Insulin/IGF-I plus TSH or insulin/IGF-I plus norepinephrine can increase both proto-oncogene expression and [3H]thymidine incorporation into DNA to the same extent; however, the former combination can increase cell number whereas the latter cannot. Tritium 109-111 insulin-like growth factor 1 Rattus norvegicus 34-39 3478712-4 1987 Within 2 min of stimulation of neutrophils with 10(-6) M PAF, the 7-fold increase in acetyltransferase activity was coincident with substantial PAF synthesis (as measured by [3H]acetate incorporation into PAF), which was 14% of the PAF synthesis induced by the Ca2+ ionophore A23187 at 10(-5) M. PAF activation of the acetyltransferase and PAF synthesis required intact neutrophils as they did not occur in cells broken by sonication. Tritium 175-177 PCNA clamp associated factor Homo sapiens 57-60 7800163-2 1994 A large number of binding sites for [3H]8-OH-DPAT were observed in the hippocampus, especially the dentate gyrus, CA1+CA2 field, dorsal raphe nucleus and septum. Tritium 37-39 carbonic anhydrase 1 Rattus norvegicus 114-117 8477871-2 1993 The stimulation of [3H]-thymidine incorporation by insulin-like growth factor I in the cells exposed to high concentrations (55 mmol/l) of glucose (4.6 +/- 1.3 fold stimulation) was significantly suppressed as compared with that in the cells cultured in 11 mmol/l glucose (17.5 +/- 0.8 fold). Tritium 20-22 insulin-like growth factor 1 Rattus norvegicus 51-79 8510491-2 1993 Maximum stimulation of [3H] thymidine incorporation of about 2.6-fold above control was reached with 100 ng/ml IGF-1 and 500 ng/ml IGF-2, respectively. Tritium 24-26 insulin-like growth factor 1 Rattus norvegicus 111-116 7598792-2 1994 p53 expression was evaluated histochemically at various stages of tumor progression (during a period of 40 weeks) that can be followed by colonic hyperproliferation labeled by 3H-thymidine incorporation. Tritium 176-178 Wistar clone pR53P1 p53 pseudogene Rattus norvegicus 0-3 3121222-4 1987 Intriguingly, maximal receptor upregulation or increase in cell size was achieved at a lower concentration (50 u/ml) of rIL-2 than was required to achieve maximal 3H-thymidine incorporation. Tritium 163-165 interleukin 2 Rattus norvegicus 120-125 8108457-3 1993 Tritium-labelled saxitoxin ([3H]-STX) binding in md optic nerve was approximately 30% greater, per wet mass of tissue, than in the control optic nerve. Tritium 0-7 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 Rattus norvegicus 33-36 8108457-3 1993 Tritium-labelled saxitoxin ([3H]-STX) binding in md optic nerve was approximately 30% greater, per wet mass of tissue, than in the control optic nerve. Tritium 29-31 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 Rattus norvegicus 33-36 8253813-4 1993 In addition, the rank order of potency for prostaglandins in competition for [3H]PGE2 specific binding to membranes prepared from EP1 cDNA transfected COS cells was PGE2 > PGE1 > PGF2 alpha > PGD2. Tritium 78-80 prostaglandin E receptor 1 Homo sapiens 130-133 8483838-5 1993 Effects of AMGZ on ovine nasal mucosal viability and transport were determined from changes in electrical properties of fluxes of [3H]GHRP, [3H]Man, and LY. Tritium 131-133 ghrelin and obestatin prepropeptide Homo sapiens 134-138 7685432-3 1993 VLA-5- myeloma cells were morphologically immature and proliferated markedly with response to IL-6 in vitro, while VLA-5+ cells showed very low uptakes of 3H-TdR but secreted higher amounts of M-protein in vitro. Tritium 155-157 integrin alpha 5 (fibronectin receptor alpha) Mus musculus 115-120 8444085-4 1993 In cell culture, gastrin treatment increased WiDr cell number and [3H]thymidine incorporation in the presence or absence of serum. Tritium 67-69 gastrin Homo sapiens 17-24 8473832-5 1993 In a culture system using two interstitial cell preparations of different buoyant densities from immature rats, both EGF and transforming growth factor-alpha (TGF-alpha) caused increased [3H]thymidine incorporation in the less dense cell preparation. Tritium 188-190 epidermal growth factor Rattus norvegicus 117-120 8005619-9 1993 Since no known cell line contains the serotonin 5-HT1A receptor (5-HT1A-R), stable transfection of the selected cell lines with a DNA construct encoding the human 5-HT1A-R was carried out and this resulted in a late increase of [3H] 8-OH-DPAT binding in the stationary phase only in the cell lines of neural origin. Tritium 229-231 5-hydroxytryptamine receptor 1A Homo sapiens 163-171 8226823-5 1993 Furthermore, activin-A and TGF-beta caused additive inhibition of capillary endothelial cell [3H]methylthymidine uptake. Tritium 94-96 inhibin subunit beta E Homo sapiens 13-20 8238515-5 1993 Specific binding of [3H]CCK-8 to isolated chief cell membranes was displaced fully by both CCK and gastrin, indicating full receptor occupancy by both peptides. Tritium 21-23 gastrin Homo sapiens 99-106 2957134-8 1987 In vitro addition of rIL-2 resulted in an enhanced AMLR in both young and aging subjects; however, the total [3H]thymidine incorporation by T cells and T-cell subsets from aging in the presence of rIL-2 was lower than that of young subjects" T cells and T-cell subsets in the presence of rIL-2. Tritium 110-112 interleukin 2 Rattus norvegicus 21-26 8286569-2 1993 When the cells were made quiescent by serum depletion, [3H]thymidine incorporation was increased by the addition of insulin plus epidermal growth factor (EGF), reaching a peak after 24 h of stimulation. Tritium 56-58 pro-epidermal growth factor Cavia porcellus 129-152 8286569-2 1993 When the cells were made quiescent by serum depletion, [3H]thymidine incorporation was increased by the addition of insulin plus epidermal growth factor (EGF), reaching a peak after 24 h of stimulation. Tritium 56-58 pro-epidermal growth factor Cavia porcellus 154-157 8479545-3 1993 In addition, differentiation of 5-HT1D receptors was achieved by incubation of the tissues with [3H]-5-HT in the presence of 100 nmol/l 8-OH-DPAT together with 100 nmol/l mesulergine. Tritium 97-99 5-hydroxytryptamine receptor 1D Cavia porcellus 32-38 8479545-4 1993 Areas presenting high densities of 5-HT1A receptors included the neocortex (internal layers), hippocampal formation (dentate gyrus, CA1 field), septum and raphe nuclei, while 5-HT1C sites accounted for most of the [3H]-5-HT binding to the choroid plexus. Tritium 215-217 5-hydroxytryptamine receptor 1A Cavia porcellus 35-41 8219218-7 1993 The addition of anti-M-CSF or anti-M-CSF receptor neutralizing antibodies dose-dependently inhibited the binding of [3H]-thymidine-labeled FDC-P2-MCSFR cells to KM102 cells. Tritium 117-119 colony stimulating factor 1 Homo sapiens 21-26 2957134-8 1987 In vitro addition of rIL-2 resulted in an enhanced AMLR in both young and aging subjects; however, the total [3H]thymidine incorporation by T cells and T-cell subsets from aging in the presence of rIL-2 was lower than that of young subjects" T cells and T-cell subsets in the presence of rIL-2. Tritium 110-112 interleukin 2 Rattus norvegicus 197-202 8219218-7 1993 The addition of anti-M-CSF or anti-M-CSF receptor neutralizing antibodies dose-dependently inhibited the binding of [3H]-thymidine-labeled FDC-P2-MCSFR cells to KM102 cells. Tritium 117-119 colony stimulating factor 1 Homo sapiens 35-40 2957134-8 1987 In vitro addition of rIL-2 resulted in an enhanced AMLR in both young and aging subjects; however, the total [3H]thymidine incorporation by T cells and T-cell subsets from aging in the presence of rIL-2 was lower than that of young subjects" T cells and T-cell subsets in the presence of rIL-2. Tritium 110-112 interleukin 2 Rattus norvegicus 197-202 8439537-2 1993 At 37 degrees C as well as 4 degrees C, [3H]retinol uptake from [3H]retinol-RBP showed a time-dependent increase, and was not saturable at concentrations exceeding the physiological concentration by more than a factor of 2 (3 microM). Tritium 41-43 retinol binding protein 4 Rattus norvegicus 76-79 8439537-2 1993 At 37 degrees C as well as 4 degrees C, [3H]retinol uptake from [3H]retinol-RBP showed a time-dependent increase, and was not saturable at concentrations exceeding the physiological concentration by more than a factor of 2 (3 microM). Tritium 65-67 retinol binding protein 4 Rattus norvegicus 76-79 2957134-9 1987 The net increase (over the baseline values) in [3H]thymidine incorporation in T-TA and CD8-TA by rIL-2 was significantly less in the aging group when compared to the young group. Tritium 48-50 interleukin 2 Rattus norvegicus 97-102 8439537-8 1993 Uptake characteristics of [3H]retinol from CRBP and lactoglobulin were similar to that of [3H]retinol from RBP. Tritium 27-29 retinol binding protein 4 Rattus norvegicus 44-47 8223875-5 1993 Stimulation of [3H]PA production upon CD3 cross-linking was 77% lower in permeabilized CD45- cells than in CD45+ cells, consistent with the reduced activity of p59fyn in CD45- cells. Tritium 16-18 FYN proto-oncogene, Src family tyrosine kinase Homo sapiens 160-166 2957134-10 1987 In contrast, the rIL-2-induced net increase in [3H]thymidine incorporation in CD4-TA AMLR in both groups was comparable. Tritium 48-50 interleukin 2 Rattus norvegicus 17-22 8450942-2 1993 The 3H outflow, evoked by 3,4-DAP or veratridine, was inhibited by tetrodotoxin (TTX) or omega-CTX but the 3H outflow evoked by monensin was neither affected by TTX nor by omega-CTX in Ca(2+)-free medium, containing 1 mM EGTA. Tritium 4-6 death-associated protein Rattus norvegicus 30-33 7906471-2 1993 We have therefore studied the NPY-induced release of [3H]-GABA, [3H]-glycine, [3H]-dopamine, [3H]-5-hydroxytryptamine, and [3H]-choline chloride-derived radioactivity in the rabbit and chicken retina. Tritium 54-56 neuropeptide Y Oryctolagus cuniculus 30-33 2820395-1 1987 We prepared unlabeled and 3H-labeled analogs of platelet-activating factor (PAF) containing a N-methylcarbamyl residue at the sn-2 position. Tritium 26-28 PCNA clamp associated factor Homo sapiens 48-74 7906471-2 1993 We have therefore studied the NPY-induced release of [3H]-GABA, [3H]-glycine, [3H]-dopamine, [3H]-5-hydroxytryptamine, and [3H]-choline chloride-derived radioactivity in the rabbit and chicken retina. Tritium 54-57 neuropeptide Y Oryctolagus cuniculus 30-33 7906471-2 1993 We have therefore studied the NPY-induced release of [3H]-GABA, [3H]-glycine, [3H]-dopamine, [3H]-5-hydroxytryptamine, and [3H]-choline chloride-derived radioactivity in the rabbit and chicken retina. Tritium 65-67 neuropeptide Y Oryctolagus cuniculus 30-33 7906471-3 1993 NPY affected the release of [3H]-glycine, [3H]-dopamine, [3H]-5-hydroxytryptamine, and [3H]-choline chloride-derived radioactivity in rabbit retina and of [3H]-GABA, [3H]-5-hydroxytryptamine and [3H]-choline chloride-derived radioactivity in chicken retina in an energy requiring, NA+K(+)-ATPase dependent and calcium dependent manner. Tritium 29-32 neuropeptide Y Oryctolagus cuniculus 0-3 7906471-3 1993 NPY affected the release of [3H]-glycine, [3H]-dopamine, [3H]-5-hydroxytryptamine, and [3H]-choline chloride-derived radioactivity in rabbit retina and of [3H]-GABA, [3H]-5-hydroxytryptamine and [3H]-choline chloride-derived radioactivity in chicken retina in an energy requiring, NA+K(+)-ATPase dependent and calcium dependent manner. Tritium 29-31 neuropeptide Y Oryctolagus cuniculus 0-3 7906471-3 1993 NPY affected the release of [3H]-glycine, [3H]-dopamine, [3H]-5-hydroxytryptamine, and [3H]-choline chloride-derived radioactivity in rabbit retina and of [3H]-GABA, [3H]-5-hydroxytryptamine and [3H]-choline chloride-derived radioactivity in chicken retina in an energy requiring, NA+K(+)-ATPase dependent and calcium dependent manner. Tritium 43-45 neuropeptide Y Oryctolagus cuniculus 0-3 7906471-3 1993 NPY affected the release of [3H]-glycine, [3H]-dopamine, [3H]-5-hydroxytryptamine, and [3H]-choline chloride-derived radioactivity in rabbit retina and of [3H]-GABA, [3H]-5-hydroxytryptamine and [3H]-choline chloride-derived radioactivity in chicken retina in an energy requiring, NA+K(+)-ATPase dependent and calcium dependent manner. Tritium 43-45 neuropeptide Y Oryctolagus cuniculus 0-3 7906471-3 1993 NPY affected the release of [3H]-glycine, [3H]-dopamine, [3H]-5-hydroxytryptamine, and [3H]-choline chloride-derived radioactivity in rabbit retina and of [3H]-GABA, [3H]-5-hydroxytryptamine and [3H]-choline chloride-derived radioactivity in chicken retina in an energy requiring, NA+K(+)-ATPase dependent and calcium dependent manner. Tritium 43-45 neuropeptide Y Oryctolagus cuniculus 0-3 7906471-3 1993 NPY affected the release of [3H]-glycine, [3H]-dopamine, [3H]-5-hydroxytryptamine, and [3H]-choline chloride-derived radioactivity in rabbit retina and of [3H]-GABA, [3H]-5-hydroxytryptamine and [3H]-choline chloride-derived radioactivity in chicken retina in an energy requiring, NA+K(+)-ATPase dependent and calcium dependent manner. Tritium 43-45 neuropeptide Y Oryctolagus cuniculus 0-3 8216230-3 1993 GLUT4 correlated very strongly (r2 = 0.97) with maximal insulin-stimulated glucose uptake (Rg" max., estimated using the glucose clamp plus 2-deoxy[3H]glucose bolus technique) in six skeletal muscles and heart. Tritium 148-150 solute carrier family 2 member 4 Rattus norvegicus 0-5 8244097-3 1993 HGT-1 cells exhibited [3H]omeprazole binding in the non-stimulated state, which increased 100% in the presence of 1 mM histamine. Tritium 23-25 solute carrier family 25 member 16 Homo sapiens 0-5 8450181-2 1993 PCP inhibited [3H]-thymidine incorporation in lymphocytes treated with PHA or IL-2. Tritium 15-17 interleukin 2 Mus musculus 78-82 7680260-4 1993 [3H]Kainate binding was highest in the dentate gyrus followed by field CA3 and then field CA1, the ratio of the highest to the lowest being 3 to 2. Tritium 1-3 carbonic anhydrase 1 Rattus norvegicus 90-93 7680260-5 1993 Binding of [3H]DL-alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) differed, to a certain extent, from that of [3H]kainate and showed the dentate gyrus followed by field CA1 and then field CA3 in the rank order of decreasing binding. Tritium 12-14 carbonic anhydrase 1 Rattus norvegicus 181-184 2820395-1 1987 We prepared unlabeled and 3H-labeled analogs of platelet-activating factor (PAF) containing a N-methylcarbamyl residue at the sn-2 position. Tritium 26-28 PCNA clamp associated factor Homo sapiens 76-79 8503284-1 1993 3,4-Diaminopyridine (3,4-DAP 100 mumol.L-1) evoked [3H]norepinephrine ([3H]NE) release in rat hippocampal slices preincubated with [3H]NE and superfused with medium with or without Ca2+. Tritium 52-54 death-associated protein Rattus norvegicus 25-28 8376986-1 1993 Mesencephalic cell cultures were used as a model to investigate the effects of interleukin-2 (IL-2) on evoked release of [3H]dopamine ([3H]DA) and gamma-[3H]-aminobutyric acid ([3H]GABA). Tritium 122-124 interleukin 2 Rattus norvegicus 79-92 8376986-1 1993 Mesencephalic cell cultures were used as a model to investigate the effects of interleukin-2 (IL-2) on evoked release of [3H]dopamine ([3H]DA) and gamma-[3H]-aminobutyric acid ([3H]GABA). Tritium 122-124 interleukin 2 Rattus norvegicus 94-98 8376986-1 1993 Mesencephalic cell cultures were used as a model to investigate the effects of interleukin-2 (IL-2) on evoked release of [3H]dopamine ([3H]DA) and gamma-[3H]-aminobutyric acid ([3H]GABA). Tritium 136-138 interleukin 2 Rattus norvegicus 94-98 8503284-1 1993 3,4-Diaminopyridine (3,4-DAP 100 mumol.L-1) evoked [3H]norepinephrine ([3H]NE) release in rat hippocampal slices preincubated with [3H]NE and superfused with medium with or without Ca2+. Tritium 72-74 death-associated protein Rattus norvegicus 25-28 2439316-7 1987 In mitogenesis assays performed on human fibroblasts isolated from the skin of two fetuses of an early gestational age, 15K IGF-II stimulated the incorporation of [3H]thymidine into DNA at a half-maximal concentration, i.e. ED50, of 5.7 and 5.0 nM. Tritium 164-166 insulin like growth factor 2 Homo sapiens 124-130 1334753-2 1992 The effect of agents which interact with the histamine H3 receptor on potassium-stimulated tritium release from slices of rat entorhinal cortex preloaded with [3H]-choline is described. Tritium 91-98 histamine receptor H3 Rattus norvegicus 45-66 1334753-2 1992 The effect of agents which interact with the histamine H3 receptor on potassium-stimulated tritium release from slices of rat entorhinal cortex preloaded with [3H]-choline is described. Tritium 160-162 histamine receptor H3 Rattus norvegicus 45-66 8376986-2 1993 At low concentrations (10(-13)-10(-12) M), IL-2 potentiated [3H]DA release evoked by the excitatory amino acids N-methyl-D-aspartate (NMDA) and kainate, whereas higher IL-2 concentrations (10(-9)-10(-8) M) had no effect. Tritium 61-63 interleukin 2 Rattus norvegicus 43-47 7507605-9 1993 EGF, IGF-I (Insulin-like Growth Factor-I), or PDGF (Platelet-derived Growth Factor) alone increased [3H]thymidine incorporation in the cells. Tritium 101-103 insulin like growth factor 1 Gallus gallus 5-10 7507605-9 1993 EGF, IGF-I (Insulin-like Growth Factor-I), or PDGF (Platelet-derived Growth Factor) alone increased [3H]thymidine incorporation in the cells. Tritium 101-103 insulin like growth factor 1 Gallus gallus 12-40 7507605-10 1993 Adding IGF-I or PDGF simultaneously with EGF enhanced the response of the cells to EGF by increasing [3H]thymidine incorporation. Tritium 102-104 insulin like growth factor 1 Gallus gallus 7-12 3302538-9 1987 The observed reduction in molecular size upon chymotrypsin treatment was confirmed when analysed by SDS-polyacrylamide gel electrophoresis after covalently labelling of the AR with [3H]R1881. Tritium 182-184 androgen receptor Bos taurus 173-175 1360027-4 1992 The affinity and selectivity of these compounds for D-1 and D-2 subtypes was determined in radioligand competition assays for the DA receptors of rat striatum membranes using [3H]SCH 23390 (D-1 selective) and [3H]spiperone (D-2 selective) as radioligands. Tritium 176-178 solute carrier family 3 member 1 Rattus norvegicus 52-63 1360027-4 1992 The affinity and selectivity of these compounds for D-1 and D-2 subtypes was determined in radioligand competition assays for the DA receptors of rat striatum membranes using [3H]SCH 23390 (D-1 selective) and [3H]spiperone (D-2 selective) as radioligands. Tritium 210-212 solute carrier family 3 member 1 Rattus norvegicus 52-63 8396016-10 1993 Insulin and IGF-I treatment of cultured luteal cells for 18 h increased [3H]thymidine incorporation (6- to 7-fold). Tritium 73-75 insulin Bos taurus 0-7 3034668-3 1987 Enzymatic studies using [3H][D-Ala2, Leu]enkephalin as substrate confirmed the presence of NEP in dura and pia mater of human tissue. Tritium 25-27 prodynorphin Homo sapiens 37-51 8396016-10 1993 Insulin and IGF-I treatment of cultured luteal cells for 18 h increased [3H]thymidine incorporation (6- to 7-fold). Tritium 73-75 insulin like growth factor 1 Bos taurus 12-17 8248864-6 1993 As measured by [3H]inositol monophosphate ([3H]InsP1) accumulation, normal rat seru, ET-1, and ET-2 stimulated phosphoinositide hydrolysis 47%, 420%, and 154% above the basal rate observed in serum free controls. Tritium 16-18 endothelin 2 Rattus norvegicus 95-99 8248864-6 1993 As measured by [3H]inositol monophosphate ([3H]InsP1) accumulation, normal rat seru, ET-1, and ET-2 stimulated phosphoinositide hydrolysis 47%, 420%, and 154% above the basal rate observed in serum free controls. Tritium 44-46 endothelin 2 Rattus norvegicus 95-99 8344470-2 1993 Antisense EGF ODN resulted in > 90% inhibition of EGF immunoreactive peptide synthesis, 75% reduction in branching morphogenesis, 73% decrease in DNA content, 64% decrease in RNA content, 73% decrease in protein synthesis, and 65% decrease in [3H]thymidine incorporation into DNA compared to Embryonic Day 11 controls in culture for 4 days. Tritium 247-249 epidermal growth factor Mus musculus 10-13 7689100-1 1993 A431 cells, a human epidermoid carcinoma, possess specific [3H]platelet-activating factor (PAF) and [3H]WEB 2086 binding sites indicating the presence of PAF receptors. Tritium 60-62 PCNA clamp associated factor Homo sapiens 154-157 1359791-5 1992 Low concentrations of CNP (20 x 10(-9) M) inhibit up to 80% of the [3H]-thymidine incorporation induced by basic fibroblast growth factor, platelet derived growth factor, epidermal growth factor (EGF), and heparin binding EGF-like growth factor. Tritium 68-70 natriuretic peptide C Bos taurus 22-25 1331399-0 1992 Characterization of [3H]morphine binding to interleukin-1-activated thymocytes. Tritium 21-23 interleukin 1 alpha Homo sapiens 44-57 1331399-1 1992 We have previously reported that interleukin-1-induced proliferation of thymocytes is accompanied by the appearance of [3H]morphine binding sites on these cells. Tritium 120-122 interleukin 1 alpha Homo sapiens 33-46 1331399-5 1992 These [3H]morphine binding sites may mediate a negative feedback effect on interleukin-1-induced proliferation of thymocytes in vivo. Tritium 7-9 interleukin 1 alpha Homo sapiens 75-88 1400315-7 1992 With intact differentiated HL-60 cells, [3H]acetate from [3H]PAF can be incorporated into alk-1-enylacetyl-GPE in the presence of ionophore A23187, but not in its absence. Tritium 41-43 PCNA clamp associated factor Homo sapiens 61-64 1400315-7 1992 With intact differentiated HL-60 cells, [3H]acetate from [3H]PAF can be incorporated into alk-1-enylacetyl-GPE in the presence of ionophore A23187, but not in its absence. Tritium 41-43 activin A receptor like type 1 Homo sapiens 90-95 1431676-8 1992 T3 (3 micrograms/l) inhibited the increase in [3H]thymidine incorporation caused by 25 micrograms IGF-I/l (51 +/- 13% compared with IGF-I-treated culture). Tritium 47-49 insulin-like growth factor 1 Rattus norvegicus 98-103 8338843-3 1993 All assays gave good agreement except the filter binding assay of [3H]-GDP bound to p21, which gave values of 35-40% compared to the other methods. Tritium 67-69 H3 histone pseudogene 16 Homo sapiens 84-87 3567925-10 1987 This concentration of ANF was observed to inhibit the metabolism of [3H]B(a)P by splenocyte cultures to water soluble metabolites. Tritium 69-71 natriuretic peptide type A Mus musculus 22-25 8374738-0 1993 Apparent regional differences in 5-HT1A binding may reflect [3H]8-OH-DPAT labeling of serotonin uptake sites. Tritium 61-63 5-hydroxytryptamine receptor 1A Bos taurus 33-39 1527040-1 1992 Platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine, PAF) labeled with 3H in the alkyl side chain was taken up rapidly by amnion-derived WISH cells in culture. Tritium 94-96 PCNA clamp associated factor Homo sapiens 76-79 3587508-7 1987 In addition, [3H]GABA, exogenous, and GABA, endogenous, responded to external stimulae (Ca2+, veretradine, various GABA concentrations and the glutaminase inhibitor diazo-nor-leucine) in a way which was compatible with them being localized in and/or released from different compartments. Tritium 14-16 glutaminase Homo sapiens 143-154 1385182-6 1992 However activation of L-type channels is not involved in EAA-induced [3H]DA release and therefore inhibition of EAA-induced [3H]DA release by micromolar concentrations of DHPs must be mediated by actions other than inhibition of L-type Ca2+ channels. Tritium 125-127 deoxyhypusine synthase Rattus norvegicus 171-175 1324156-3 1992 We now have studied the effects of T3 by itself and in combination with TSH and insulin-like growth factor-I (IGF-I) on [3H]thymidine incorporation into DNA, iodide uptake, and cAMP production in FRTL5. Tritium 121-123 insulin-like growth factor 1 Rattus norvegicus 110-115 1324156-10 1992 T3 potentiated the [3H]thymidine response to 2 and 10 ng/ml IGF-I by 1.5- to 1.7-fold. Tritium 20-22 insulin-like growth factor 1 Rattus norvegicus 60-65 7687625-3 1993 In these studies, we evaluated three events in the interaction of Escherichia coli [3H]LPS with human PMN: 1) binding to the plasma membrane; 2) translocation to an intracellular compartment; and 3) enzymatic deacylation. Tritium 84-86 interferon regulatory factor 6 Homo sapiens 87-90 3558401-4 1987 Upon incubation in the presence of [3H]retinol-RBP, isolated plasma membrane fractions take up and esterify retinol. Tritium 36-38 retinol binding protein 4 Bos taurus 47-50 8325370-3 1993 Moreover, EGF could stimulate the formation of [3H]phosphatidic acid in [3H]myristic acid preloaded cells, suggesting that EGF is able to activate cellular phospholipase D. Also, PMA was able to increase the phosphatidic acid formation with a parallel increase in the adenylate cyclase activity. Tritium 48-50 epidermal growth factor Bos taurus 10-13 8325370-3 1993 Moreover, EGF could stimulate the formation of [3H]phosphatidic acid in [3H]myristic acid preloaded cells, suggesting that EGF is able to activate cellular phospholipase D. Also, PMA was able to increase the phosphatidic acid formation with a parallel increase in the adenylate cyclase activity. Tritium 48-50 epidermal growth factor Bos taurus 123-126 1334723-2 1992 This property has been applied to the measurement of PAF in circulating blood, the concept being that in vivo contact with endogenous PAF diminishes subsequent in vitro binding of 3H-labeled PAF. Tritium 180-182 PCNA clamp associated factor Homo sapiens 53-56 1334723-2 1992 This property has been applied to the measurement of PAF in circulating blood, the concept being that in vivo contact with endogenous PAF diminishes subsequent in vitro binding of 3H-labeled PAF. Tritium 180-182 PCNA clamp associated factor Homo sapiens 134-137 1334723-2 1992 This property has been applied to the measurement of PAF in circulating blood, the concept being that in vivo contact with endogenous PAF diminishes subsequent in vitro binding of 3H-labeled PAF. Tritium 180-182 PCNA clamp associated factor Homo sapiens 134-137 1334723-3 1992 Here we show that, when platelets are stimulated with thrombin, the subsequent binding of [3H]PAF is greatly reduced, probably due to proteolytic damage of the PAF receptors. Tritium 91-93 PCNA clamp associated factor Homo sapiens 94-97 1334723-3 1992 Here we show that, when platelets are stimulated with thrombin, the subsequent binding of [3H]PAF is greatly reduced, probably due to proteolytic damage of the PAF receptors. Tritium 91-93 PCNA clamp associated factor Homo sapiens 160-163 8400115-3 1993 The appearance of PAF was assessed by measuring the number of freely accessible PAF-receptors on platelets in vitro, assuming that the contact between platelets and PAF in vivo would reduce the receptor binding of [3H]PAF in vitro. Tritium 215-217 PCNA clamp associated factor Homo sapiens 18-21 1334723-4 1992 In addition, [3H]PAF binding is decreased, when the cell"s energy status is low, which is seen during thrombin-induced secretion. Tritium 14-16 PCNA clamp associated factor Homo sapiens 17-20 3548429-8 1987 [3H]LHRH was cleaved by BBM or by endopeptidase-24.11 from porcine PT to metabolites 2, 4, small amounts of 3, and less than Glu-His-Trp-Ser-Tyr-Gly, but cleavage was strongly inhibited by the specific inhibitor phosphoramidon. Tritium 1-3 gonadotropin releasing hormone 1 Rattus norvegicus 4-8 1500712-8 1992 Continued OKT3-induced activation for 20 to 72 h resulted in a further increase in phosphorylated Op18 forms, which paralleled new Op18 synthesis and occurred at a time when cells were entering S-phase, as determined by [3H]-thymidine incorporation. Tritium 221-223 stathmin 1 Homo sapiens 98-102 1353458-2 1992 PCNA complex formation was detected by the immunofluorescence method after methanol fixation and nucleotide excision repair activity was detected as the unscheduled DNA synthesis (UDS) by autoradiography labeled with [3H]thymidine. Tritium 218-220 proliferating cell nuclear antigen Homo sapiens 0-4 1322431-4 1992 Both IL-1 alpha and beta inhibited [3H] thymidine uptake into NPA cell DNA in a dose- and time-dependent manner at concentrations ranging from 5 x 10(2) to 10(5) U/L; 10(5) U/L of IL-1 suppressed DNA synthesis by more than 40% of control. Tritium 36-38 interleukin 1 alpha Homo sapiens 5-15 1353889-3 1992 [3H]Glycine transport mediated by clone GLYT1 is blocked by sarcosine but is not blocked by methyl-aminoisobutyric acid or L-alanine, a substrate specificity similar to that described for a previously identified glycine-uptake system called system Gly. Tritium 1-3 solute carrier family 6 member 9 L homeolog Xenopus laevis 40-45 1377689-2 1992 Carbohydrate recognition by the human endothelial-leukocyte adhesion molecule, E-selectin, has been investigated by binding studies using 3H-labeled Chinese hamster ovary cells expressing different levels of the transfected full-length adhesion molecule and a series of structurally defined oligosaccharides linked to the lipid phosphatidylethanolamine dipalmitoate (neoglycolipids) and synthetic glycolipids chromatographed on silica gel plates or immobilized on plastic wells. Tritium 138-140 selectin E Homo sapiens 79-89 1322127-5 1992 In contrast, when serine was substituted for the last cysteine in the RAS extension, transfer of the [3H]farnesyl group from [3H] farnesyl pyrophosphate to the modified Ub-cRAS was not observed. Tritium 102-104 ubiquitin Oryctolagus cuniculus 169-171 1637948-2 1992 The mechanism of phosphatidylcholine (PC) degradation stimulated by PAF was investigated in endometrial explants prelabeled with [methyl-3H]choline or preincubated with [3H]butan-1-ol. Tritium 137-139 PCNA clamp associated factor Homo sapiens 68-71 1637948-3 1992 Analysis of the water-soluble metabolites of PAF-induced PC hydrolysis in secretory endometrium demonstrated that the stimulated generation of [3H]choline ([3H]Cho) precedes that of [3H]choline phosphate ([3H]ChoP) and [3H]glycerophosphocholine ([3H]GPC). Tritium 144-146 PCNA clamp associated factor Homo sapiens 45-48 1637948-3 1992 Analysis of the water-soluble metabolites of PAF-induced PC hydrolysis in secretory endometrium demonstrated that the stimulated generation of [3H]choline ([3H]Cho) precedes that of [3H]choline phosphate ([3H]ChoP) and [3H]glycerophosphocholine ([3H]GPC). Tritium 157-159 PCNA clamp associated factor Homo sapiens 45-48 1637948-3 1992 Analysis of the water-soluble metabolites of PAF-induced PC hydrolysis in secretory endometrium demonstrated that the stimulated generation of [3H]choline ([3H]Cho) precedes that of [3H]choline phosphate ([3H]ChoP) and [3H]glycerophosphocholine ([3H]GPC). Tritium 157-159 PCNA clamp associated factor Homo sapiens 45-48 1637948-3 1992 Analysis of the water-soluble metabolites of PAF-induced PC hydrolysis in secretory endometrium demonstrated that the stimulated generation of [3H]choline ([3H]Cho) precedes that of [3H]choline phosphate ([3H]ChoP) and [3H]glycerophosphocholine ([3H]GPC). Tritium 157-159 PCNA clamp associated factor Homo sapiens 45-48 1637948-4 1992 Within 30 sec there was a rapid rise in PAF-induced [3H]Cho generation and by 2 min this had increased to 59.9% +/- 10.6% (p less than 0.02), with no effect upon [3H]ChoP and [3H]GPC during this period. Tritium 53-55 PCNA clamp associated factor Homo sapiens 40-43 1381344-3 1992 Biological and pharmacological properties of WS9326A and tetrahydro-WS9326A (FK224) WS9326A binds competitively to [3H]substance P (NK-1 receptor) binding sites on guinea-pig lung membranes (IC50 = 3.6 x 10(-6) M), and acts as a tachykinin antagonist in various functional assays. Tritium 116-118 substance-P receptor Cavia porcellus 132-145 1407001-2 1992 In lung preparations in which COMT and MAO were inhibited, the uptake of 3H-labelled dopamine, (-)-adrenaline and (-)-noradrenaline, but not (+/-)-isoprenaline, was reduced by cocaine (10 or 100 mumol/l). Tritium 73-75 catechol-O-methyltransferase Rattus norvegicus 30-34 1616931-2 1992 Introduction of the transferase modifier, alpha-lactalbumin, or galactosyltransferase-associated kinase inhibitor trifluoperazine, into beta-agonist-treated rats prevented acinar cell proliferation as determined by [3H]thymidine incorporation after 96 h of treatment. Tritium 216-218 lactalbumin, alpha Rattus norvegicus 42-59 1425452-3 1992 IL-1 by itself enhanced [3H]thymidine incorporation in a concentration-dependent manner. Tritium 25-27 interleukin 1 alpha Homo sapiens 0-4 1425452-4 1992 Moreover, IL-1 acted synergistically with insulin, epidermal growth factor (EGF), or fibroblast growth factor (FGF) to enhance [3H]thymidine incorporation. Tritium 128-130 interleukin 1 alpha Homo sapiens 10-14 1425452-4 1992 Moreover, IL-1 acted synergistically with insulin, epidermal growth factor (EGF), or fibroblast growth factor (FGF) to enhance [3H]thymidine incorporation. Tritium 128-130 epidermal growth factor Homo sapiens 51-74 1425452-4 1992 Moreover, IL-1 acted synergistically with insulin, epidermal growth factor (EGF), or fibroblast growth factor (FGF) to enhance [3H]thymidine incorporation. Tritium 128-130 epidermal growth factor Homo sapiens 76-79 1350980-1 1992 Activated human T cells via the CD2 or the CD3 pathways exhibited a higher capacity than resting T lymphocytes to incorporate and metabolize [3H]pafacether (paf) at 37 degrees C. Resting T lymphocytes lacked specific binding capacity for paf, yet high-affinity paf receptors (paf-R) were induced on CD3- or CD2-dependent activation. Tritium 142-144 CD2 molecule Homo sapiens 32-35 1613390-9 1992 In an [3H]thymidine incorporation assay with BM cells collected at different times of culture, d3 or d4 BM cells responded to human recombinant M-CSF, human recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF), bovine GM-CSF, murine M-CSF or murine M-CSF-containing supernatants, and bovine interleukin 1 beta (IL-1 beta) in decreasing order of magnitude. Tritium 7-9 colony stimulating factor 2 Homo sapiens 169-217 1382660-4 1992 Quantitative autoradiography reveals that PLA2-treatment produces a differential increase in [3H]-AMPA binding across brain regions. Tritium 94-96 phospholipase A2 group IB Rattus norvegicus 42-46 1378422-4 1992 Of the three clones producing antibodies which bound to LTB4-R, 0.5 mg/ml of one IgG3k antibody, termed E2, precipitated over 90% of the [3H]LTB4-binding activity of solubilized PMN leucocyte membrane proteins. Tritium 138-140 immunoglobulin heavy constant gamma 3 (G3m marker) Homo sapiens 81-85 1374070-6 1992 Recombinant IGF-I and IGF-II, and conditioned medium from 3T3-J2 cells, caused a dose-dependent increase of 3H-thymydine incorporation in cultured keratinocytes. Tritium 108-110 insulin-like growth factor 2 Mus musculus 22-28 1352896-6 1992 Following thrombin-induced platelet activation, there was rapid formation of 3H-labeled inositol phosphates (IPs) with inositol monophosphate (IP1) being the most abundant product. Tritium 77-79 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 143-146 1351426-3 1992 Whereas PLA2 treatment of telencephalic membranes from postnatal day (PND) 5 and 10 animals produced an important decrease in [3H]AMPA binding, the same treatment performed on PND 20, 25 and adult membranes resulted in a marked increase in [3H]AMPA binding; the shift from decreased to increased [3H]AMPA binding occurred at about PND 15. Tritium 127-129 phospholipase A2 group IB Rattus norvegicus 8-12 1351426-3 1992 Whereas PLA2 treatment of telencephalic membranes from postnatal day (PND) 5 and 10 animals produced an important decrease in [3H]AMPA binding, the same treatment performed on PND 20, 25 and adult membranes resulted in a marked increase in [3H]AMPA binding; the shift from decreased to increased [3H]AMPA binding occurred at about PND 15. Tritium 241-243 phospholipase A2 group IB Rattus norvegicus 8-12 1351426-3 1992 Whereas PLA2 treatment of telencephalic membranes from postnatal day (PND) 5 and 10 animals produced an important decrease in [3H]AMPA binding, the same treatment performed on PND 20, 25 and adult membranes resulted in a marked increase in [3H]AMPA binding; the shift from decreased to increased [3H]AMPA binding occurred at about PND 15. Tritium 241-243 phospholipase A2 group IB Rattus norvegicus 8-12 1351426-4 1992 In contrast to [3H]AMPA binding, [3H]6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) binding was substantially reduced following PLA2 treatment at PND 5, 10 and 20, and effect due to a decrease in the maximal number of [3H]CNQX binding sites. Tritium 34-36 phospholipase A2 group IB Rattus norvegicus 125-129 1312103-4 1992 Furthermore, L-659,989 (10 microM), as well as PAF (1 microM), inhibited specific [3H]PAF binding in LPS-treated but not in untreated cells. Tritium 83-85 PCNA clamp associated factor Homo sapiens 47-50 1312103-4 1992 Furthermore, L-659,989 (10 microM), as well as PAF (1 microM), inhibited specific [3H]PAF binding in LPS-treated but not in untreated cells. Tritium 83-85 PCNA clamp associated factor Homo sapiens 86-89 1533270-6 1992 The shortest diamines (DA2 and DA3) acted as weak partial agonists, enhancing the binding of [3H[MK-801. Tritium 94-96 piezo type mechanosensitive ion channel component 2 Homo sapiens 31-34 1550204-2 1992 EGF induced dose-dependent [3H]Thd uptake (P less than 0.001); after 10(-9) M EGF, DNA synthesis rate peaked at 24 h, averaging 77% of the response to 10% FCS, and then declined steeply with nadir at 48-60 h. Unexpectedly, EGF failed to induce cell proliferation in the first 4 days, leaving this initial burst of DNA synthesis (12-60 h) uncoupled from cell division. Tritium 28-30 epidermal growth factor Homo sapiens 0-3 1614979-5 1992 However, storage of an rIL-2/poloxamer formulation at 37 degrees C for 72 hr resulted in an approximate 15% reduction in the biological activity as assessed by [3H]thymidine incorporation. Tritium 161-163 interleukin 2 Rattus norvegicus 23-28 1371281-3 1992 Acid hydrolysis of CK8 and CK18, purified from [3H]glucosamine-labeled cells, generated free glucosamine. Tritium 48-50 keratin 18 Homo sapiens 27-31 1311083-4 1992 The receptor antagonists inhibited release of intracellular calcium elicited by NPY in human erythroleukemia cells and displaced 3H-labeled NPY from NPY receptors in rat brain membrane. Tritium 129-131 neuropeptide Y Homo sapiens 140-143 1311083-4 1992 The receptor antagonists inhibited release of intracellular calcium elicited by NPY in human erythroleukemia cells and displaced 3H-labeled NPY from NPY receptors in rat brain membrane. Tritium 129-131 neuropeptide Y Homo sapiens 140-143 8100589-3 1993 Beginning with postnatal day 8, high binding levels were confined mostly to the hippocampal strata: the oriens and radiatum (CA1 and CA3 with [3H]MK 801 labeling but only CA1 with NMDA displaced [3H]glutamate labeling), the moleculare (higher labeling with [3H]MK 801 than with NMDA displaced [3H]glutamate binding), and the lucidum. Tritium 143-145 carbonic anhydrase 1 Mus musculus 125-128 8496167-6 1993 Addition of phospholipase A2 (PLA2) inhibitors (quinacrine, 4-bromophenacyl bromide, manoalide) to the perfusion medium inhibited K(+)-stimulated [3H]ACh and [3H]AA release in a dose-dependent manner. Tritium 147-149 phospholipase A2 group IB Rattus norvegicus 12-28 8496167-6 1993 Addition of phospholipase A2 (PLA2) inhibitors (quinacrine, 4-bromophenacyl bromide, manoalide) to the perfusion medium inhibited K(+)-stimulated [3H]ACh and [3H]AA release in a dose-dependent manner. Tritium 147-149 phospholipase A2 group IB Rattus norvegicus 30-34 8496167-6 1993 Addition of phospholipase A2 (PLA2) inhibitors (quinacrine, 4-bromophenacyl bromide, manoalide) to the perfusion medium inhibited K(+)-stimulated [3H]ACh and [3H]AA release in a dose-dependent manner. Tritium 159-161 phospholipase A2 group IB Rattus norvegicus 12-28 8496167-6 1993 Addition of phospholipase A2 (PLA2) inhibitors (quinacrine, 4-bromophenacyl bromide, manoalide) to the perfusion medium inhibited K(+)-stimulated [3H]ACh and [3H]AA release in a dose-dependent manner. Tritium 159-161 phospholipase A2 group IB Rattus norvegicus 30-34 8097236-5 1993 The association and dissociation rates of [3H]L-689,560 binding were decreased by CPP and D-2-amino-5-phosphonopentanoic acid ("C-5"). Tritium 43-45 complement C5 Rattus norvegicus 128-131 8478984-9 1993 Co-incubation in IFN-gamma (20-100 U/ml) and IGF-II (3-10 nM) prevented the inhibitory effects of IFN-gamma on [3H]TdR incorporation in serum-free media. Tritium 112-114 insulin like growth factor 2 Homo sapiens 45-51 8097600-5 1993 Incorporation of [3H]TdR was the parameter most sensitive to BaP with an IC50 relative to controls of 4.5 +/- 3.1 x 10(-8) M with a range for individuals of 1.8 x 10(-8) to 6.6 x 10(-8) M (n = 15). Tritium 18-20 prohibitin 2 Homo sapiens 61-64 8449921-3 1993 After 48 h, bFGF (0.1-10 nM) inhibited the incorporation of [3H]proline into collagenase-digestible protein (CDP). Tritium 61-63 cut-like homeobox 1 Mus musculus 77-107 8449921-3 1993 After 48 h, bFGF (0.1-10 nM) inhibited the incorporation of [3H]proline into collagenase-digestible protein (CDP). Tritium 61-63 cut-like homeobox 1 Mus musculus 109-112 7681811-11 1993 The addition of IGF-I monoclonal antibody to rabbit adipocyte precursor cells cultured in serum-free medium significantly inhibited [3H]-thymidine incorporation and significantly decreased (50%) GPDH specific activities. Tritium 133-135 insulin-like growth factor I Oryctolagus cuniculus 16-21 8423097-7 1993 Like human lactoferrin, bovine lactoferrin and lactoferricin have the ability to bind to free intrinsically labeled [3H]LPS molecules. Tritium 117-119 lactotransferrin Bos taurus 11-22 8423097-7 1993 Like human lactoferrin, bovine lactoferrin and lactoferricin have the ability to bind to free intrinsically labeled [3H]LPS molecules. Tritium 117-119 lactotransferrin Bos taurus 31-42 8442433-7 1993 TNF-alpha and TNF-beta (100 ng/ml) stimulated bone matrix breakdown, as assessed by analysis of the release of 3H from bone prelabeled with [3H]proline. Tritium 111-113 lymphotoxin A Mus musculus 14-22 8442433-7 1993 TNF-alpha and TNF-beta (100 ng/ml) stimulated bone matrix breakdown, as assessed by analysis of the release of 3H from bone prelabeled with [3H]proline. Tritium 141-143 lymphotoxin A Mus musculus 14-22 8474535-9 1993 In line with this hypothesis, the accumulation of the preferential MAO-A substrate [3H]-5-HT in caudate nucleus slices from pigmented rabbits was about 39% lower than that in slices from albino rabbits. Tritium 84-86 amine oxidase [flavin-containing] A Oryctolagus cuniculus 67-72 8431776-2 1993 EB significantly decreased [3H]paroxetine binding in male and female rats in the oriens layers of CA1-CA4, and in the radiata/lacunosum moleculare layers of CA2 and CA3. Tritium 28-30 carbonic anhydrase 1 Rattus norvegicus 98-101 8430834-13 1993 Radioligand binding studies with the specific 5-HT1A receptor ligand [3H]8-OH-DPAT confirmed the presence of 5-HT1A receptor binding sites in bulk-isolated rat medullary TAL. Tritium 70-72 5-hydroxytryptamine receptor 1A Homo sapiens 46-61 8430834-13 1993 Radioligand binding studies with the specific 5-HT1A receptor ligand [3H]8-OH-DPAT confirmed the presence of 5-HT1A receptor binding sites in bulk-isolated rat medullary TAL. Tritium 70-72 5-hydroxytryptamine receptor 1A Homo sapiens 109-124 7679031-2 1993 The potency of the non-peptide antagonists CP-96,345 and RP 67580 on NK1 receptor-stimulated [3H]-inositol phosphate accumulation in cell lines or tissue from three different species has been examined. Tritium 94-96 tachykinin receptor 1 Homo sapiens 69-81 8261576-3 1993 Mechanism studies showed that endogenous PARP activity (incorporation of tritiated nicotinamide adenine dinucleotide, [3H]-NAD) was 2.6 times higher in O-342/DDP than in O-342 cells and that the presence of 2.5 mM NA during the incubation with the isotope resulted in 73.3% inhibition of the enzyme activity in O-342/DDP cells but in only about 30% inhibition in the sensitive line. Tritium 119-121 poly (ADP-ribose) polymerase 1 Rattus norvegicus 41-45 7679066-1 1993 The genes encoding esterase D (ESD) and alpha 2-macroglobulin (A2M) were mapped using 3H-labeled cDNAs to sheep chromosome 3p26-->p24 and 3q26-->q35 respectively. Tritium 86-88 S-formylglutathione hydrolase Ovis aries 19-29 7679066-1 1993 The genes encoding esterase D (ESD) and alpha 2-macroglobulin (A2M) were mapped using 3H-labeled cDNAs to sheep chromosome 3p26-->p24 and 3q26-->q35 respectively. Tritium 86-88 S-formylglutathione hydrolase Ovis aries 31-34 7679066-1 1993 The genes encoding esterase D (ESD) and alpha 2-macroglobulin (A2M) were mapped using 3H-labeled cDNAs to sheep chromosome 3p26-->p24 and 3q26-->q35 respectively. Tritium 86-88 alpha-2-macroglobulin Ovis aries 40-61 7679066-1 1993 The genes encoding esterase D (ESD) and alpha 2-macroglobulin (A2M) were mapped using 3H-labeled cDNAs to sheep chromosome 3p26-->p24 and 3q26-->q35 respectively. Tritium 86-88 alpha-2-macroglobulin Ovis aries 63-66 8104445-0 1993 Correlation between [3H]thymidine and proliferating cell nuclear antigen (PCNA)/cyclin indices in archival, formaldehyde-fixed human colorectal tissues. Tritium 21-23 proliferating cell nuclear antigen Homo sapiens 74-78 8104445-5 1993 Finally, there was a highly significant correlation (r = 0.898; P < 0.0001) between the [3H]thymidine labelling index (TLI) and the proportion of PCNA-positive nuclei (PCNAF-LI). Tritium 92-94 proliferating cell nuclear antigen Homo sapiens 149-153 8419407-7 1993 A synthetic peptide from another cationic region of SPARC (peptide 2.3) increased [3H]-thymidine incorporation by BAE cells and fibroblasts in a dose-dependent manner. Tritium 83-85 secreted protein acidic and cysteine rich Bos taurus 52-57 8069578-8 1993 The permeability coefficients of the RIP-bile acid conjugates, determined using Caco-2 cell monolayers, were shown to be six times less than that of [3H]-PEG (800-1000). Tritium 150-152 receptor interacting serine/threonine kinase 1 Homo sapiens 37-40 7678040-3 1993 PLA2 pretreatment induced a significant increase in alpha-[3H]amino-3-hydroxy-5-methyl-isoxazole-4-propionate ([3H]AMPA) binding in the stratum radiatum of the CA1 region of the hippocampus and in the stratum moleculare of the cerebellum. Tritium 59-61 phospholipase A2 group IB Rattus norvegicus 0-4 7678040-5 1993 [3H]-Glutamate binding to the metabotropic glutamate receptor and the non-NMDA-, non-kainate-, non-quisqualate-sensitive [3H]glutamate binding site were also increased by PLA2 pretreatment. Tritium 1-3 phospholipase A2 group IB Rattus norvegicus 171-175 7678040-5 1993 [3H]-Glutamate binding to the metabotropic glutamate receptor and the non-NMDA-, non-kainate-, non-quisqualate-sensitive [3H]glutamate binding site were also increased by PLA2 pretreatment. Tritium 122-124 phospholipase A2 group IB Rattus norvegicus 171-175 8445991-2 1993 Since a strong correlation has been reported between B-50 (phosphorylation) and [3H]NA release from intact or streptolysin-O- (SL-O-) permeated cortical synaptosomes, we investigated whether the effects of ACTH-(1-24) on the release of radiolabelled transmitters are mediated by B-50. Tritium 81-83 growth associated protein 43 Homo sapiens 53-57 1447188-4 1992 Leukosialin was immunoprecipitated from [3H]glucosamine-labeled HL-60 and K562 cells. Tritium 41-43 LOC105369247 Homo sapiens 0-11 1361417-7 1992 In cortical areas Par1/Par2, [3H]L-689,560 was also significantly reduced in deep layers but only in rats receiving 6 ng volkensin. Tritium 30-32 F2R like trypsin receptor 1 Rattus norvegicus 23-27 1332696-5 1992 However, tracking studies showed that [3H]PAF accumulated on the cell surface for approximately 2 min before being internalized. Tritium 39-41 PCNA clamp associated factor Homo sapiens 42-45 1450138-1 1992 OBJECTIVE: To determine the metabolism of 3H-Platelet-activating factor (3H-PAF) during transfer through human fetal membranes. Tritium 42-44 PCNA clamp associated factor Homo sapiens 76-79 1282913-10 1992 Accordingly, [3H]thymidine incorporation in HMEC was optimally stimulated by EGF at concentrations of 10(-11) to 10(-10) M. HMEC were also seen to possess functional VIP receptors linked to the adenylate cyclase system, as we previously observed in seven human breast cancer cell lines. Tritium 14-16 epidermal growth factor Homo sapiens 77-80 1468887-5 1992 Addition of recombinant IL-2 in vitro to mixed lymphocyte reactions (MLR) using irradiated (500 cGy) allogeneic responder spleen cells augmented the 3H-TdR uptake fourfold compared to cultures without IL-2, but was 44-fold below the allogeneic response of nonirradiated C57BL/6 spleen cells. Tritium 149-151 interleukin 2 Mus musculus 24-28 1468887-5 1992 Addition of recombinant IL-2 in vitro to mixed lymphocyte reactions (MLR) using irradiated (500 cGy) allogeneic responder spleen cells augmented the 3H-TdR uptake fourfold compared to cultures without IL-2, but was 44-fold below the allogeneic response of nonirradiated C57BL/6 spleen cells. Tritium 149-151 interleukin 2 Mus musculus 201-205 1301381-9 1992 The effect of GnRH on LH glycosylation was detected earlier than that on translation, the [3H]LH rates of production and release being higher than those of [14C]LH. Tritium 91-93 gonadotropin releasing hormone 1 Rattus norvegicus 14-18 1301381-11 1992 Removal of extracellular calcium resulted in the loss of cellular responsiveness to GnRH, preventing not only the stimulatory effects of GnRH on total and released iLH but also the GnRH-induced incorporation of both [14C]leu and [3H]gln into newly synthesized LH. Tritium 230-232 gonadotropin releasing hormone 1 Rattus norvegicus 84-88 1480152-1 1992 An exogenous [3H]triolein emulsion was hydrolyzed by intact cardiac myocytes with functional LPL located on the cell surface. Tritium 14-16 lipoprotein lipase Homo sapiens 93-96 1396338-9 1992 Analysis of the time course revealed that IGF-I (10 nM) induces maximal stimulation of [3H]thymidine incorporation (a 4-fold increase) 16-18 h after exposure. Tritium 88-90 insulin-like growth factor 1 Rattus norvegicus 42-47 1396338-11 1992 The dose-response of [3H]thymidine incorporation induced by IGF-I and TPA indicated that 10 nM was the lowest concentration producing a maximal effect for both agents. Tritium 22-24 insulin-like growth factor 1 Rattus norvegicus 60-65 1396338-14 1992 When astrocytes were exposed to IGF-I (10 nM) and TPA (10 nM) in combination, [3H]thymidine uptake was significantly higher than the uptake induced by either IGF-I (10 nM) or TPA (10 nM) alone. Tritium 79-81 insulin-like growth factor 1 Rattus norvegicus 32-37 1330647-4 1992 The 5-HT1A receptor transfectants displayed a single high affinity site for the agonist [3H](+/-)-8-hydroxy-2-(di-n-propylamino)tetralin HBr ([3H]8-OH-DPAT) and a pharmacological profile specific for the 5-HT1A receptor. Tritium 89-91 5-hydroxytryptamine receptor 1A Homo sapiens 4-19 1330647-4 1992 The 5-HT1A receptor transfectants displayed a single high affinity site for the agonist [3H](+/-)-8-hydroxy-2-(di-n-propylamino)tetralin HBr ([3H]8-OH-DPAT) and a pharmacological profile specific for the 5-HT1A receptor. Tritium 89-91 5-hydroxytryptamine receptor 1A Homo sapiens 204-219 1452414-12 1992 Down-modulation of CD3 expression on CD4+ and CD8+ cells was apparent as early as 1 h after treatment with less than 10% of CD4+ and CD8+ cells expressing CD3 by 12 h. Induction of IL-2R expression and IL-2-driven 3H-TdR incorporation was maximal at 12 h after anti-CD3. Tritium 214-216 interleukin 2 Mus musculus 181-185 1388202-6 1992 In control rats [3H]retinol concentrations began to increase in plasma after 30 min due to liver secretion of retinol bound to RBP. Tritium 17-19 retinol binding protein 4 Rattus norvegicus 127-130 1388202-7 1992 In HPR-treated rats, secretion was apparently inhibited because the amount of [3H]retinol bound to RBP at 4.66 h was only 2.6% of the control level. Tritium 79-81 retinol binding protein 4 Rattus norvegicus 99-102 1412470-5 1992 Cells were incubated with [3H]B(a)P for 24 hr. Tritium 27-29 prohibitin 2 Mus musculus 30-35 1540160-2 1992 The human CSF-1 gene has previously been assigned to chromosome 5 using somatic cell hybrids, and further localized to 5q33 by in situ hybridization with a 3H labelled cDNA probe. Tritium 156-158 colony stimulating factor 1 Homo sapiens 10-15 1311516-2 1992 Anesthetized rabbits were placed on total heart bypass, and the single-pass transpulmonary metabolism of [3H]benzoyl-Phe-Ala-Pro (BPAP) by endothelial-bound angiotensin-converting enzyme (ACE) and [14C]adenosine 5"-monophosphate (AMP) by 5"-nucleotidase (NCT) was calculated before and after PMA (10 micrograms/kg iv), a dose that does not produce histologically evident endothelial damage. Tritium 106-108 angiotensin-converting enzyme Oryctolagus cuniculus 157-186 1738120-3 1992 This cellular uptake of [3H]TEA could be described by a facilitated diffusion model (Jmax = 0.57 +/- 0.08 pmol sec-1 cm-1, Km = 0.28 +/- 0.01 mmol/l). Tritium 25-27 secretory blood group 1 Rattus norvegicus 111-121 1738121-2 1992 TCV-309 specifically inhibited PAF-induced aggregation of rabbit and human platelets, and [3H]PAF binding to rabbit platelet microsomes with IC50 values of 33, 58 and 27 nM, respectively. Tritium 91-93 PCNA clamp associated factor Homo sapiens 94-97 1325802-4 1992 [3H]Bremazocine binding to kappa receptors was unchanged at 5 min and 24 h, but showed large decreases 3 h after TBI in the CA1 pyramidal layer (65%, p less than 0.05) and dentate gyrus (43%, p less than 0.05). Tritium 1-3 carbonic anhydrase 1 Rattus norvegicus 124-127 1309295-5 1992 In contrast, in situ competition studies with unlabeled 10(-6) M benzo[ghi]-perylene (B[ghi]P) resulted in the elimination of the nuclear [3H]B[a]P 4 s complex (but not the 6 s complex); however, the EROD activity and Cyp1a-1 mRNA levels in cells treated with 10(-7) M B[a]P in the presence or absence of 10(-6) M B[ghi]P were not significantly different. Tritium 139-141 cytochrome P450 family 1 subfamily A member 1 Homo sapiens 218-225 1380408-3 1992 The inhibition of c-fos expression was not specifically mediated via kinase C, since both the total RNA synthesis as measured by [3H]-uridine uptake and the fraction of serum-induced c-fos expression that was not attributable to kinase C were inhibited by doxorubicin. Tritium 130-132 FBJ osteosarcoma oncogene Mus musculus 18-23 12106309-7 1992 IGF-I also doubled the number of surviving neurons and produced a moderate, non-significant increase in [3H]thymidine incorporation by the cultures. Tritium 105-107 insulin-like growth factor 1 Rattus norvegicus 0-5 1320624-1 1992 In the human Ewing"s sarcoma cell line WE-68, saturation analysis using 3H-labelled neuropeptide Y ([3H]NPY) as the radioligand disclosed a homogeneous population of binding sites with a dissociation constant (Kd) of 4.5 nM and maximal binding capacity (B(max)) of 712 fmol/mg cell protein. Tritium 72-74 neuropeptide Y Homo sapiens 84-98 1320624-1 1992 In the human Ewing"s sarcoma cell line WE-68, saturation analysis using 3H-labelled neuropeptide Y ([3H]NPY) as the radioligand disclosed a homogeneous population of binding sites with a dissociation constant (Kd) of 4.5 nM and maximal binding capacity (B(max)) of 712 fmol/mg cell protein. Tritium 72-74 neuropeptide Y Homo sapiens 104-107 1320624-1 1992 In the human Ewing"s sarcoma cell line WE-68, saturation analysis using 3H-labelled neuropeptide Y ([3H]NPY) as the radioligand disclosed a homogeneous population of binding sites with a dissociation constant (Kd) of 4.5 nM and maximal binding capacity (B(max)) of 712 fmol/mg cell protein. Tritium 101-103 neuropeptide Y Homo sapiens 84-98 1320624-3 1992 NPY, its natural analogues and the Y1-receptor-specific peptide ligand [Leu31,Pro34]NPY inhibited [3H]NPY binding in the potency order: [Leu31,Pro34]NPY greater than or equal to human NPY greater than or equal to peptide YY (PYY) greater than salmon pancreatic polypeptide (PP) greater than human PP greater than porcine NPY13-36 much greater than NPY22-36. Tritium 99-101 neuropeptide Y Homo sapiens 0-3 1320624-3 1992 NPY, its natural analogues and the Y1-receptor-specific peptide ligand [Leu31,Pro34]NPY inhibited [3H]NPY binding in the potency order: [Leu31,Pro34]NPY greater than or equal to human NPY greater than or equal to peptide YY (PYY) greater than salmon pancreatic polypeptide (PP) greater than human PP greater than porcine NPY13-36 much greater than NPY22-36. Tritium 99-101 neuropeptide Y Homo sapiens 84-87 1320624-3 1992 NPY, its natural analogues and the Y1-receptor-specific peptide ligand [Leu31,Pro34]NPY inhibited [3H]NPY binding in the potency order: [Leu31,Pro34]NPY greater than or equal to human NPY greater than or equal to peptide YY (PYY) greater than salmon pancreatic polypeptide (PP) greater than human PP greater than porcine NPY13-36 much greater than NPY22-36. Tritium 99-101 neuropeptide Y Homo sapiens 84-87 1320624-3 1992 NPY, its natural analogues and the Y1-receptor-specific peptide ligand [Leu31,Pro34]NPY inhibited [3H]NPY binding in the potency order: [Leu31,Pro34]NPY greater than or equal to human NPY greater than or equal to peptide YY (PYY) greater than salmon pancreatic polypeptide (PP) greater than human PP greater than porcine NPY13-36 much greater than NPY22-36. Tritium 99-101 neuropeptide Y Homo sapiens 84-87 1390692-4 1992 [3H]-all-trans-Retinol (ROL) was delivered to the basal surface of the cells by RBP. Tritium 1-3 retinol binding protein 4 Bos taurus 80-83 1517217-4 1992 When basal or insulin-stimulated adipocytes were labeled with [3H]B3GL and incubated at 37 degrees C in steady state, labeled GLUT4 was rapidly reduced at the cell surface and stoichiometrically recovered in microsomes without any change in GLUT4 protein levels in either pool. Tritium 63-65 solute carrier family 2 member 4 Rattus norvegicus 126-131 3567460-2 1987 Neurotensin evoked the release of [3H]-GABA from the strips preloaded with [3H]-GABA, and the evoked release was Ca2+-dependent and tetrodotoxin-sensitive. Tritium 35-37 neurotensin/neuromedin N Cavia porcellus 0-11 1322969-1 1992 An opioid receptor agonist, [D-Ala2,Me-Phe4,Glyol5]enkephalin (DAMGE), decreased [3H]thymidine incorporation into DNA of fetal rat brain cell aggregates. Tritium 82-84 proenkephalin Rattus norvegicus 51-61 1309881-7 1992 In matched experiments, IL-1 treatment enhanced specific [3H]bradykinin binding 1.5- to 2.0-fold above that observed in untreated cells. Tritium 58-60 interleukin 1 alpha Homo sapiens 24-28 1309881-9 1992 The potencies of a series of kinin analogs and antagonists and unrelated peptides in displacing [3H]bradykinin from IL-1-treated cells correlated well with their abilities to induce prostanoid release. Tritium 97-99 interleukin 1 alpha Homo sapiens 116-120 1314311-2 1992 Mathematical analysis of single [3H]-[D-Ala2,MePhe4,Gly-ol5] enkephalin (DAGO) saturation curves suggested binding of the [3H]-ligand at one site, while displacement curves of low concentrations of [3H]-DAGO with selective mu-ligands indicated the presence of high- and low-affinity sites. Tritium 33-35 proenkephalin Rattus norvegicus 61-71 3567460-2 1987 Neurotensin evoked the release of [3H]-GABA from the strips preloaded with [3H]-GABA, and the evoked release was Ca2+-dependent and tetrodotoxin-sensitive. Tritium 76-78 neurotensin/neuromedin N Cavia porcellus 0-11 1314311-2 1992 Mathematical analysis of single [3H]-[D-Ala2,MePhe4,Gly-ol5] enkephalin (DAGO) saturation curves suggested binding of the [3H]-ligand at one site, while displacement curves of low concentrations of [3H]-DAGO with selective mu-ligands indicated the presence of high- and low-affinity sites. Tritium 123-125 proenkephalin Rattus norvegicus 61-71 1314311-4 1992 Eighty percent of [3H]-[D-Ala2,D-Leu5] enkephalin (DADLE) binding was displaced by DAGO with high affinity, indicating that a high percentage of [3H]-DADLE binding was at mu-sites. Tritium 19-21 proenkephalin Rattus norvegicus 39-49 1314311-4 1992 Eighty percent of [3H]-[D-Ala2,D-Leu5] enkephalin (DADLE) binding was displaced by DAGO with high affinity, indicating that a high percentage of [3H]-DADLE binding was at mu-sites. Tritium 146-148 proenkephalin Rattus norvegicus 39-49 1372056-4 1992 3H-thymidine uptake was markedly increased by the addition of GM-CSF in two cases, G-CSF in one, and IL-3 in two. Tritium 0-2 colony stimulating factor 2 Homo sapiens 62-68 1372056-4 1992 3H-thymidine uptake was markedly increased by the addition of GM-CSF in two cases, G-CSF in one, and IL-3 in two. Tritium 0-2 colony stimulating factor 3 Homo sapiens 83-88 1372056-4 1992 3H-thymidine uptake was markedly increased by the addition of GM-CSF in two cases, G-CSF in one, and IL-3 in two. Tritium 0-2 interleukin 3 Homo sapiens 101-105 1381381-4 1992 Anti-TCR activated cells incorporated [3H]-thymidine at a level comparable to ConA or antigen-stimulated cells and secreted interleukin (IL)-2 at comparable levels Anti-TCR activated blasts were greater than 90% positive for CD3 and alpha/beta TCR, 60% CD4+ and 30% CD8+. Tritium 39-41 T cell receptor alpha variable 6-3 Mus musculus 5-8 1381381-4 1992 Anti-TCR activated cells incorporated [3H]-thymidine at a level comparable to ConA or antigen-stimulated cells and secreted interleukin (IL)-2 at comparable levels Anti-TCR activated blasts were greater than 90% positive for CD3 and alpha/beta TCR, 60% CD4+ and 30% CD8+. Tritium 39-41 T cell receptor alpha variable 6-3 Mus musculus 169-172 1381381-4 1992 Anti-TCR activated cells incorporated [3H]-thymidine at a level comparable to ConA or antigen-stimulated cells and secreted interleukin (IL)-2 at comparable levels Anti-TCR activated blasts were greater than 90% positive for CD3 and alpha/beta TCR, 60% CD4+ and 30% CD8+. Tritium 39-41 T cell receptor alpha variable 6-3 Mus musculus 169-172 1405334-4 1992 TNF-beta, IL-1 beta and IL-2 also modestly stimulated 3H release from 3H-arachidonic acid labeled cells. Tritium 54-56 lymphotoxin alpha Sus scrofa 0-8 1405334-5 1992 Mepacrine, a phospholipase A inhibitor, prevented TNF-beta stimulation of 3H release from 3H-arachidonic acid labeled cells and TNF-beta potentiation of adenylate cyclase activity. Tritium 74-76 lymphotoxin alpha Sus scrofa 50-58 3029143-4 1987 The binding of [3H]RA-CRABP and [3H]retinol-CRBP is not dramatically affected by prior removal of the outer nuclear membrane with Triton X-100. Tritium 33-35 retinol binding protein 1, cellular Mus musculus 44-48 3029143-5 1987 However, treatment with the detergent after the binding reaction is complete removes about half of the bound [3H]RA-CRABP and almost all of the bound [3H]retinol-CRBP. Tritium 151-153 retinol binding protein 1, cellular Mus musculus 162-166 1397383-3 1992 A close similarity between cytologic localization of myc product and [3H]thymidine labeling suggests that myc expression is linked to trophoblast proliferation. Tritium 70-72 MYC proto-oncogene, bHLH transcription factor Homo sapiens 106-109 18475483-2 1992 Here, using the human IL-1 receptor antagonist (IL-1RA) and a new monoclonal antibody (mAb 7F11) to rabbit TNF, the role of endogenous IL-l and TNF production in acute (3h) leukocyte (PMNL) recruitment to dermal inflammation in rabbits has been studied. Tritium 169-171 tumor necrosis factor Oryctolagus cuniculus 135-147 2951504-2 1987 The addition of either the 5-HT1A-selective drug 8-OH-DPAT (100 nM) or the 5-HT1C-selective drug mesulergine (100 nM) to the assay resulted in a 5-10% decrease in specific 3H-5-HT binding. Tritium 172-174 5-hydroxytryptamine receptor 1A Bos taurus 27-33 1319006-8 1992 Frozen brain sections were prepared, incubated in [3H]D-Ala2,MePhe4,Gly-ol5-enkephalin, which selectively labels mu-receptors, and analyzed using quantitative receptor autoradiography. Tritium 51-53 proenkephalin Rattus norvegicus 76-86 1318633-2 1992 3,4-DAP-evoked [3H]NE release both in the absence and presence of extracellular Ca2+. Tritium 16-18 death-associated protein Rattus norvegicus 4-7 1318633-3 1992 3,4-DAP-evoked Ca(2+)-independent release of [3H]NE was highly sensitive to sodium channel blocker, tetrodotoxin (TTX, 100% inhibition), suggesting that Na+ per se entered into nerve terminals but not depolarization of membrane followed by Ca2+ channel opening is necessary for 3,4-DAP-evoked Ca(2+)-independent release of [3H]NE. Tritium 46-48 death-associated protein Rattus norvegicus 4-7 1318633-3 1992 3,4-DAP-evoked Ca(2+)-independent release of [3H]NE was highly sensitive to sodium channel blocker, tetrodotoxin (TTX, 100% inhibition), suggesting that Na+ per se entered into nerve terminals but not depolarization of membrane followed by Ca2+ channel opening is necessary for 3,4-DAP-evoked Ca(2+)-independent release of [3H]NE. Tritium 46-48 death-associated protein Rattus norvegicus 282-285 1510717-9 1992 In contrast, in cells labeled with [3H]AA, IL-1 causes the formation of DAG associated with the hydrolysis of PC. Tritium 36-38 interleukin 1 alpha Homo sapiens 43-47 2889855-1 1987 Double immunofluorescence and [3H]thymidine autoradiographic analysis of the same field of transformed human amnion cells (AMA) reacted with proliferating cell nuclear antigen (PCNA) autoantibodies and a monoclonal antibody (mAb 19F4) specific for cyclin (PCNA) revealed similar patterns and sequence of cyclin (PCNA) antigen staining during S-phase. Tritium 31-33 proliferating cell nuclear antigen Homo sapiens 177-181 1354232-4 1992 i) Hydrolysis of alkyl-acyl-GPC upon CD2 stimulation was evidenced: [3H]alkyl-lyso-GPC was formed from [3H]alkyl-acyl-GPC in [3H] alkyl-labeled cells; alkyl-lyso-GPC production was also bioassayed after CD2 triggering. Tritium 69-71 CD2 molecule Homo sapiens 37-40 1354232-4 1992 i) Hydrolysis of alkyl-acyl-GPC upon CD2 stimulation was evidenced: [3H]alkyl-lyso-GPC was formed from [3H]alkyl-acyl-GPC in [3H] alkyl-labeled cells; alkyl-lyso-GPC production was also bioassayed after CD2 triggering. Tritium 69-71 CD2 molecule Homo sapiens 203-206 1354232-4 1992 i) Hydrolysis of alkyl-acyl-GPC upon CD2 stimulation was evidenced: [3H]alkyl-lyso-GPC was formed from [3H]alkyl-acyl-GPC in [3H] alkyl-labeled cells; alkyl-lyso-GPC production was also bioassayed after CD2 triggering. Tritium 104-106 CD2 molecule Homo sapiens 37-40 1318633-3 1992 3,4-DAP-evoked Ca(2+)-independent release of [3H]NE was highly sensitive to sodium channel blocker, tetrodotoxin (TTX, 100% inhibition), suggesting that Na+ per se entered into nerve terminals but not depolarization of membrane followed by Ca2+ channel opening is necessary for 3,4-DAP-evoked Ca(2+)-independent release of [3H]NE. Tritium 324-326 death-associated protein Rattus norvegicus 4-7 1318633-4 1992 Phorbol ester PDB increased by 480% of control and polymyxin B (PMB) decreased 3,4-DAP-evoked [3H]NE release by 94% of control in the absence of extracellular Ca2+ indicating that the alterations of voltage-dependent Ca(2+)-currents into the cells are not involved in the mechanism of the modulation of 3,4-DAP-evoked [3H]NE release by protein kinase C. Tritium 95-97 death-associated protein Rattus norvegicus 83-86 1662484-5 1991 PAF enhanced [3H]glycerol incorporation into PtdOH and DG by 2- and 3-fold respectively during 1-10 min incubations. Tritium 14-16 PCNA clamp associated factor Homo sapiens 0-3 1818764-6 1991 At 72 hours, low concentrations of LIF produced less marked stimulation of [3H]-thymidine incorporation, and 5,000 u/ml actually inhibited DNA synthesis at both this time point and at 96 hours. Tritium 76-78 leukemia inhibitory factor Mus musculus 35-38 1483116-3 1992 Administration of interleukin-1 alpha to cultured osteoblasts produce an increase in cellular proliferation as suggested by 3H-thymidine incorporation and cell growth studies. Tritium 124-126 interleukin 1 alpha Homo sapiens 18-37 3495790-1 1987 The effect of EGF on the incorporation of [3H]TdR into DNA (DNA synthesis) was determined in the esophagus, liver, pancreas, and kidney in mice standardized to 12 hours (hr) of light alternating with 12 hr of darkness. Tritium 43-45 epidermal growth factor Mus musculus 14-17 1628711-1 1992 Erythropoietin (Ep) stimulated glucose uptake by erythroid progenitor cells in the liver of fetal rats, as measured by [3H]2-deoxy-D-glucose uptake. Tritium 120-122 erythropoietin Rattus norvegicus 0-14 1407369-3 1992 The IGF-II preparation used was shown to stimulate 3H-thymidine incorporation in MG-63 human osteosarcoma cells in vitro. Tritium 51-53 insulin like growth factor 2 Homo sapiens 4-10 1668105-7 1991 Scatchard analysis of [3H]PAF binding to monocyte membranes revealed 651 +/- 495 binding sites/monocyte with a Kd of 4.7 +/- 4.2 x 10(-10) M. PAF is a structurally unique activator of monocytes whose interactions with TNF alpha and other cytokines may be critical to host defense against tumors. Tritium 23-25 PCNA clamp associated factor Homo sapiens 26-29 3548718-2 1987 Incorporation of [3H]-thymidine was stimulated 1.4-2.4 fold by insulin, insulin like growth factor (IGF), platelet derived growth factor (PDGF), epidermal growth factor (EGF) and 2% fetal calf serum (FCS) respectively. Tritium 18-20 insulin-like growth factor 1 Rattus norvegicus 72-98 1824254-4 1991 IFN-gamma also inhibited M-CSF-, GM-CSF-, or IL-3-stimulated [3H]-thymidine incorporation in highly enriched GM-CFC. Tritium 62-64 colony stimulating factor 1 Homo sapiens 25-30 3548718-2 1987 Incorporation of [3H]-thymidine was stimulated 1.4-2.4 fold by insulin, insulin like growth factor (IGF), platelet derived growth factor (PDGF), epidermal growth factor (EGF) and 2% fetal calf serum (FCS) respectively. Tritium 18-20 insulin-like growth factor 1 Rattus norvegicus 100-103 1824254-4 1991 IFN-gamma also inhibited M-CSF-, GM-CSF-, or IL-3-stimulated [3H]-thymidine incorporation in highly enriched GM-CFC. Tritium 62-64 colony stimulating factor 2 Homo sapiens 33-39 1612026-6 1992 The protein kinase-A (PKA) activator 8-bromo-cAMP increased both iodide uptake and DNA synthesis; TGF beta inhibited 8-bromo-cAMP-induced [125I]iodide uptake, but not [3H]thymidine incorporation. Tritium 168-170 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 22-25 1824254-4 1991 IFN-gamma also inhibited M-CSF-, GM-CSF-, or IL-3-stimulated [3H]-thymidine incorporation in highly enriched GM-CFC. Tritium 62-64 interleukin 3 Homo sapiens 45-49 3548718-2 1987 Incorporation of [3H]-thymidine was stimulated 1.4-2.4 fold by insulin, insulin like growth factor (IGF), platelet derived growth factor (PDGF), epidermal growth factor (EGF) and 2% fetal calf serum (FCS) respectively. Tritium 18-20 myotrophin Rattus norvegicus 85-98 3598846-7 1987 [3H]Tryptamine binding was decreased by treatment with Azure A and phospholipase A2, while phospholipase D had no effect. Tritium 1-3 phospholipase A2 group IB Rattus norvegicus 67-83 1610790-2 1992 Prelabeled cells ([3H]AA) responded to GnRH challenge with increased formation (about 2-fold) of the leukotrienes LTC4, LTD4, and LTE4 as well as 5- and 15-eicosatetraenoic acids (5- and 15-HETE) as identified by HPLC. Tritium 19-21 gonadotropin releasing hormone 1 Rattus norvegicus 39-43 2947634-8 1987 The effect of IGF-I on [3H]thymidine and [35S]sulfate uptake by the tissue increased with differentiation while the effect of IGF-II on [3H]thymidine uptake of the undifferentiated tissue was abolished with differentiation of the tissue. Tritium 137-139 insulin-like growth factor 2 Mus musculus 126-132 1596568-2 1992 Exposure to IL-3, IL-7, IL-1, and IL-6 resulted in a mean 2.8-, 1.5-, 1.4-, and 1.6-fold stimulation of 3H-thymidine (3H-TdR) incorporation, respectively. Tritium 104-106 interleukin 3 Homo sapiens 12-16 1799225-2 1991 This method can potentially be adapted to prepare tritiated IGF-II with the use of tritium gas in the hydrogenolysis step. Tritium 83-90 insulin like growth factor 2 Homo sapiens 60-66 1813180-4 1991 Based on [3H]PAF-binding studies, an obstruction of PAF-binding or the signal transduction by the branched alkyl chain in C-1 position of the glycerol backbone is suggested. Tritium 10-12 PCNA clamp associated factor Homo sapiens 13-16 1596568-2 1992 Exposure to IL-3, IL-7, IL-1, and IL-6 resulted in a mean 2.8-, 1.5-, 1.4-, and 1.6-fold stimulation of 3H-thymidine (3H-TdR) incorporation, respectively. Tritium 104-106 interleukin 1 alpha Homo sapiens 24-28 3542986-6 1987 This distribution of 3H suggested that 80% of apoB was cleaved at three places. Tritium 21-23 apolipoprotein B Gallus gallus 46-50 1586169-9 1992 The Kc cell 23.5-kDa GTP-binding protein"s 3H-labeled oligopeptide(s)/amino acid(s) was geranylgeranylated and its tryptic digest profile was representative of prenylated proteins whose oligopeptides eluted with greater than or equal to 93% acetonitrile. Tritium 43-45 Signal recognition particle receptor alpha Drosophila melanogaster 21-40 1587186-8 1992 [3H]Leucine incorporation studies in vivo showed more labeling of immunoprecipitable LPH than SI during starvation, but refeeding induced relatively more labeling of SI than of LPH. Tritium 1-3 lactase Rattus norvegicus 85-88 1813180-4 1991 Based on [3H]PAF-binding studies, an obstruction of PAF-binding or the signal transduction by the branched alkyl chain in C-1 position of the glycerol backbone is suggested. Tritium 10-12 PCNA clamp associated factor Homo sapiens 52-55 1787448-3 1991 A lower but significant increase in [3H]thymidine incorporation was elicited by epidermal growth factor (EGF, 100 ng/ml) or insulin (10 micrograms/ml) added to the basal medium. Tritium 37-39 pro-epidermal growth factor Cavia porcellus 80-103 3545205-5 1987 When DNA synthesis in PDGF-treated competent cells is assessed by measuring [3H]thymidine incorporation, IGF-II by itself has only a small effect. Tritium 77-79 insulin-like growth factor 2 Mus musculus 105-111 1525356-3 1992 The dose range of unlabeled PAF required to produce inhibition of [3H]PAF binding was roughly the same as that for induction of chemotaxis, and was much higher than that in platelets as reported in the literature. Tritium 67-69 PCNA clamp associated factor Homo sapiens 28-31 3545205-7 1987 By contrast, in competent cells briefly treated with EGF, IGF-II causes a marked stimulation of [3H]thymidine incorporation. Tritium 97-99 insulin-like growth factor 2 Mus musculus 58-64 1525356-3 1992 The dose range of unlabeled PAF required to produce inhibition of [3H]PAF binding was roughly the same as that for induction of chemotaxis, and was much higher than that in platelets as reported in the literature. Tritium 67-69 PCNA clamp associated factor Homo sapiens 70-73 3113151-2 1987 A recombinant IL-2 (rIL-2) and an IL-2 containing conditioned medium (LyIL-2) markedly enhanced 3H-thymidine incorporations at low PHA concentrations, whereas at optimal mitogen concentrations, this effect was marginal. Tritium 96-98 interleukin 2 Rattus norvegicus 20-25 1534843-7 1992 In cortical areas, Par1/Par2 [3H]8-OH-DPAT binding was significantly reduced (2 and 6 ng animals) in both superficial and deeper cortical layers, but quantitative histological analysis has not been performed. Tritium 30-32 F2R like trypsin receptor 1 Rattus norvegicus 24-28 1376379-3 1992 Increased 3H-thymidine uptake was generally observed after IL3 treatment. Tritium 10-12 interleukin 3 Homo sapiens 59-62 1924354-5 1991 Additional steps in Ha-ras p21 processing, normally initiated by farnesylation, appear to be supported by geranylgeranylation, based on metabolic labeling of Ha-ras p21(Leu-189) with [3H]palmitate and its subcellular localization in a particulate fraction from COS cells. Tritium 184-186 H3 histone pseudogene 16 Homo sapiens 27-30 1924354-5 1991 Additional steps in Ha-ras p21 processing, normally initiated by farnesylation, appear to be supported by geranylgeranylation, based on metabolic labeling of Ha-ras p21(Leu-189) with [3H]palmitate and its subcellular localization in a particulate fraction from COS cells. Tritium 184-186 H3 histone pseudogene 16 Homo sapiens 165-168 1910817-4 1991 Analyses of hydrolytic products in BPAEC radiolabeled with [3H]myo-inositol and exposed to Cd2+ indicate that degradation of complex phospholipids is mediated by phospholipase A2. Tritium 59-63 LOC104974671 Bos taurus 162-178 1914309-8 1991 Although 14C of cellular lysophosphatidylcholine (lyso-PC) was not increased significantly, the 3H seemed to be released from the 2-position of PC by the action of phospholipase A2. Tritium 96-98 phospholipase A2 Oryctolagus cuniculus 164-180 1915099-4 1991 Autoradiography of [3H]thymidine incorporation in EGF-treated cultures showed that more than 80% of nuclei in mother follicles and migrating cells were labeled after 4 days. Tritium 20-22 epidermal growth factor Homo sapiens 50-53 1321958-1 1992 Inhibition of [3H]-5-hydroxytryptamine ([3H]-5-HT) release from guinea pig brain slices via activation of the terminal 5-HT autoreceptor has previously been characterised as a model of 5-HT1D receptor activation, based on the rank potencies of a range of agonists, and the potent antagonism of the inhibitory effects of 5-HT by metitepine. Tritium 15-17 5-hydroxytryptamine receptor 1D Cavia porcellus 185-200 2444471-5 1987 This synthetic (I-16) peptide was found to selectively inhibit the IL-2-dependent uptake of 3H-TDR by CTLL cells, apparently by competing with IL-2 for the IL-2 receptor. Tritium 92-94 interleukin 2 Mus musculus 67-71 1321958-1 1992 Inhibition of [3H]-5-hydroxytryptamine ([3H]-5-HT) release from guinea pig brain slices via activation of the terminal 5-HT autoreceptor has previously been characterised as a model of 5-HT1D receptor activation, based on the rank potencies of a range of agonists, and the potent antagonism of the inhibitory effects of 5-HT by metitepine. Tritium 41-43 5-hydroxytryptamine receptor 1D Cavia porcellus 185-200 1654389-4 1991 As shown by nonlinear least-squares analysis, the mu opioids Tyr-D-Ala-Gly-(Me)Phe-Gly-ol or sufentanil abolished high-affinity [3H]naltrexone binding, whereas the delta-selective ligands [D-Pen2,D-Pen5]enkephalin, ICI 174,864, and oxymorphindole inhibited and eventually eliminated the low-affinity component in a concentration-dependent manner. Tritium 129-131 proenkephalin Rattus norvegicus 203-213 2444471-5 1987 This synthetic (I-16) peptide was found to selectively inhibit the IL-2-dependent uptake of 3H-TDR by CTLL cells, apparently by competing with IL-2 for the IL-2 receptor. Tritium 92-94 interleukin 2 Mus musculus 143-147 3028377-15 1986 The only 3H-labelled product identified was phosphatidic acid, suggesting that the [3H]mfVSG-cleaving activity is a phospholipase D. These data strongly support the proposal that production of low Mr alkaline phosphatase during butanol extraction is an autolytic process occurring as the result of an endogenous phospholipase. Tritium 84-86 AT695_RS04080 Staphylococcus aureus 200-220 1795022-2 1991 HL-60 cells differentiated to macrophages (HL-60/M phi) with a phorbol ester convert added [3H]PAF to AAG; 22% of the incorporated radioactivity is converted to AAG within 15s. Tritium 92-94 PCNA clamp associated factor Homo sapiens 95-98 1659542-2 1991 Human, bovine and avian growth hormone (GH) as well as insulin-like growth factor I (IGF-I) attenuated the appearance of [3H]CDP in the medium without affecting [3H]NCDP. Tritium 122-124 insulin like growth factor 1 Bos taurus 55-83 1659542-2 1991 Human, bovine and avian growth hormone (GH) as well as insulin-like growth factor I (IGF-I) attenuated the appearance of [3H]CDP in the medium without affecting [3H]NCDP. Tritium 122-124 insulin like growth factor 1 Bos taurus 85-90 1659542-2 1991 Human, bovine and avian growth hormone (GH) as well as insulin-like growth factor I (IGF-I) attenuated the appearance of [3H]CDP in the medium without affecting [3H]NCDP. Tritium 162-164 insulin like growth factor 1 Bos taurus 55-83 1582808-5 1992 Of the various compounds examined, only interleukin-1 (IL-1) and transforming growth factor (TGF)-beta significantly stimulated [3H]GAG accumulation in a dose- and time-dependent fashion. Tritium 129-131 interleukin 1 alpha Homo sapiens 40-53 1582808-5 1992 Of the various compounds examined, only interleukin-1 (IL-1) and transforming growth factor (TGF)-beta significantly stimulated [3H]GAG accumulation in a dose- and time-dependent fashion. Tritium 129-131 interleukin 1 alpha Homo sapiens 55-59 1582808-7 1992 In conclusion, both IL-1 and TGF-beta are potent stimulators of [3H]GAG accumulation by retroocular connective tissue and perimysial fibroblasts, as well as by fibroblasts from the dermal sites studied. Tritium 65-67 interleukin 1 alpha Homo sapiens 20-24 1659542-2 1991 Human, bovine and avian growth hormone (GH) as well as insulin-like growth factor I (IGF-I) attenuated the appearance of [3H]CDP in the medium without affecting [3H]NCDP. Tritium 162-164 insulin like growth factor 1 Bos taurus 85-90 1304366-4 1992 When pyruvate kinase was reacted with 120 microM DBBD at pH 7.0 for 50 min in the absence of protectants, 1.8 mol of tritium/mol of subunit was incorporated, whereas in the presence of phosphoenolpyruvate with KCl, only 1.0 mol of tritium was incorporated per mole of subunit. Tritium 117-124 pyruvate kinase PKLR Oryctolagus cuniculus 5-20 3097648-3 1986 IL-2 inhibited both [3H]thymidine incorporation and increase in cell number. Tritium 21-23 interleukin 2 Rattus norvegicus 0-4 1304366-4 1992 When pyruvate kinase was reacted with 120 microM DBBD at pH 7.0 for 50 min in the absence of protectants, 1.8 mol of tritium/mol of subunit was incorporated, whereas in the presence of phosphoenolpyruvate with KCl, only 1.0 mol of tritium was incorporated per mole of subunit. Tritium 231-238 pyruvate kinase PKLR Oryctolagus cuniculus 5-20 1585261-6 1992 Pretreatment of the membranes with phospholipase A2 invariably decreased [3H]SR 95531 binding in the frontal cortex and cerebellum. Tritium 74-76 phospholipase A2 group IB Rattus norvegicus 35-51 1585261-10 1992 Pretreatment with phospholipase A2 affected the binding of [3H]GABA and [3H]SR 95531 in these cells, as in the case of the cerebellum. Tritium 60-62 phospholipase A2 group IB Rattus norvegicus 18-34 1585261-10 1992 Pretreatment with phospholipase A2 affected the binding of [3H]GABA and [3H]SR 95531 in these cells, as in the case of the cerebellum. Tritium 73-75 phospholipase A2 group IB Rattus norvegicus 18-34 1585261-11 1992 These effects of phospholipase A2 on the binding of [3H]GABA and [3H]SR 95531 were partially prevented by the addition of delipidated bovine serum albumin. Tritium 53-55 phospholipase A2 group IB Rattus norvegicus 17-33 1869822-2 1991 AM of healthy volunteers incubated with recombinant human GM-CSF revealed incorporation of [3H]thymidine in vitro. Tritium 92-94 colony stimulating factor 2 Homo sapiens 58-64 3463413-11 1986 The inhibition of AdoHcyase perturbs levels of transmethylation metabolites and the incorporation of [3H]methyl into 5-methyl-cytosine of DNA. Tritium 102-104 adenosylhomocysteinase Homo sapiens 18-27 1834309-7 1991 Areas almost exclusively containing 5-HT1B or 5-HT1D sites, such as the ventral pallidum, globus pallidus and substantia nigra, expressed markedly more [3H]5-HT binding in rat as compared to guinea pig, while the opposite occurred in claustrum, dorsal endopiriform nucleus, lateral geniculate nucleus, and superficial grey layer of the superior colliculus. Tritium 153-155 5-hydroxytryptamine receptor 1D Rattus norvegicus 46-52 1713156-10 1991 Purified IGFBP-4 inhibited the binding of [125I]IGF-I by its receptor and blunted stimulation of [3H]thymidine incorporation by IGF-I. Tritium 98-100 insulin-like growth factor 1 Rattus norvegicus 128-133 1713161-5 1991 Incubation of cells with IGF-I resulted in time- and dose-dependent decreases in [125I]IGF-I binding and IGF-I stimulated [3H]aminoisobutyric acid uptake and [3H]thymidine incorporation. Tritium 123-125 insulin like growth factor 1 Bos taurus 25-30 1855478-4 1991 OCT inhibited the proliferation of both ER-positive (MCF-7, T-47D, and ZR-75-1) and ER-negative breast cancer cells (MDA-MB-231 and BT-20) in vitro in a time- and dose-dependent manner, as determined by cell number and [3H]thymidine uptake. Tritium 220-222 plexin A2 Homo sapiens 0-3 1665190-5 1991 Equimolar concentrations (i.e., 10(-6) M) of [Met5]enkephalin or U69,593, but not DPDPE or DAGO, suppressed the rate of [3H]thymidine incorporation by GFAP-immunoreactive, flat (type 1) astrocytes. Tritium 121-123 glial fibrillary acidic protein Mus musculus 151-155 1585261-11 1992 These effects of phospholipase A2 on the binding of [3H]GABA and [3H]SR 95531 were partially prevented by the addition of delipidated bovine serum albumin. Tritium 66-68 phospholipase A2 group IB Rattus norvegicus 17-33 1536944-3 1992 The in vitro proliferation of myeloid cell lines in serum-containing medium or proliferation of these cells induced by IL-1 in serum-free medium (measured by 3H-TdR) were inhibited by the M20 IL-1 inhibitor. Tritium 158-160 interleukin 1 receptor antagonist Homo sapiens 192-206 1587149-3 1992 [3H]-Reductively methylated rhodopsin (specific activity, 32 Ci/mmole) was suitable for use in radioimmunoassays for rhodopsin, having many advantages over radioiodinated rhodopsin for this purpose. Tritium 1-3 rhodopsin Bos taurus 28-37 1587149-3 1992 [3H]-Reductively methylated rhodopsin (specific activity, 32 Ci/mmole) was suitable for use in radioimmunoassays for rhodopsin, having many advantages over radioiodinated rhodopsin for this purpose. Tritium 1-3 rhodopsin Bos taurus 117-126 1587149-3 1992 [3H]-Reductively methylated rhodopsin (specific activity, 32 Ci/mmole) was suitable for use in radioimmunoassays for rhodopsin, having many advantages over radioiodinated rhodopsin for this purpose. Tritium 1-3 rhodopsin Bos taurus 117-126 1349168-5 1992 These results are in complete agreement with the autoradiographic localization of [3H]thymidine incorporation and demonstrate that immunoreactivity for PCNA accurately defines areas of proliferative activity in routinely processed placental tissues. Tritium 83-85 proliferating cell nuclear antigen Homo sapiens 152-156 1650834-4 1991 In competition studies, RP 59227 produced dextral and concentration-dependent shifts of the sigmoidal inhibition curve of [3H]PAF binding by PAF(C16). Tritium 123-125 PCNA clamp associated factor Homo sapiens 126-129 3533520-3 1986 In primary cultures of rat uterine cells (from 21-day-old rats), ER is degraded with a half-life of 3-4 h, as determined by pulse-chase experiments in which ER in cells are covalently labeled with [3H]TAZ (20 nM) and then exposed to a chase of 10(-6) M estradiol. Tritium 198-200 estrogen receptor 1 Rattus norvegicus 65-67 1650834-4 1991 In competition studies, RP 59227 produced dextral and concentration-dependent shifts of the sigmoidal inhibition curve of [3H]PAF binding by PAF(C16). Tritium 123-125 PCNA clamp associated factor Homo sapiens 141-148 1324692-6 1992 The order of potency for inhibition of [3H]thymidine uptake by WEHI-3B and HL-60 cells was (R)-ET-16-OCH3-GPC = (S)-ET-16-OCH3-GPC greater than (S)-PAF greater than (R)-PAF; the EC50 values were 2, 2, 15, and greater than 40 microM, respectively. Tritium 40-42 PCNA clamp associated factor Homo sapiens 148-151 1324692-6 1992 The order of potency for inhibition of [3H]thymidine uptake by WEHI-3B and HL-60 cells was (R)-ET-16-OCH3-GPC = (S)-ET-16-OCH3-GPC greater than (S)-PAF greater than (R)-PAF; the EC50 values were 2, 2, 15, and greater than 40 microM, respectively. Tritium 40-42 PCNA clamp associated factor Homo sapiens 169-172 1859412-2 1991 It was shown that not only in vivo injections of SMS 201-995, but also, unexpectedly, in vitro incubation with bombesin inhibited [3H]-thymidine incorporation. Tritium 131-133 spermine synthase Rattus norvegicus 49-52 3533520-6 1986 Analysis of nuclear ER on sodium dodecyl sulfate-polyacrylamide gels during the chase period reveals the loss of the 65,000 mol wt [3H]TAZ-labeled receptor species; no lower mol wt [3H]TAZ-labeled fragments are observed. Tritium 132-134 estrogen receptor 1 Rattus norvegicus 20-22 1643272-2 1992 [3H]PAF added to the cell lysate was similarly distributed after chromatography. Tritium 1-3 PCNA clamp associated factor Homo sapiens 4-7 3022720-3 1986 This effect was noted in the absence of changes in either total cell count or [3H]-thymidine incorporation and was reversed by co-incubation with purified rabbit thymus actin (100 micrograms/ml) but not bovine serum albumin at the same concentration. Tritium 79-81 actin Oryctolagus cuniculus 169-174 1310281-2 1992 Treatment of anterior pituitary reaggregate cell cultures from 14-day-old female rats with physiological doses of the gonadotropin-releasing hormone LHRH or neuropeptide Y (NPY) for 40 h dose-dependently increased [3H]thymidine ([3H]T) incorporation into DNA of cells expressing PRL immunoreactivity (PRL-ir) and of those expressing ACTH-ir, whereas these peptides decreased the number of [3H]T-labeled cells expressing GH-ir. Tritium 215-217 gonadotropin releasing hormone 1 Rattus norvegicus 149-153 1310281-2 1992 Treatment of anterior pituitary reaggregate cell cultures from 14-day-old female rats with physiological doses of the gonadotropin-releasing hormone LHRH or neuropeptide Y (NPY) for 40 h dose-dependently increased [3H]thymidine ([3H]T) incorporation into DNA of cells expressing PRL immunoreactivity (PRL-ir) and of those expressing ACTH-ir, whereas these peptides decreased the number of [3H]T-labeled cells expressing GH-ir. Tritium 230-232 gonadotropin releasing hormone 1 Rattus norvegicus 149-153 1310281-2 1992 Treatment of anterior pituitary reaggregate cell cultures from 14-day-old female rats with physiological doses of the gonadotropin-releasing hormone LHRH or neuropeptide Y (NPY) for 40 h dose-dependently increased [3H]thymidine ([3H]T) incorporation into DNA of cells expressing PRL immunoreactivity (PRL-ir) and of those expressing ACTH-ir, whereas these peptides decreased the number of [3H]T-labeled cells expressing GH-ir. Tritium 230-232 gonadotropin releasing hormone 1 Rattus norvegicus 149-153 1834306-1 1991 Quantitative autoradiographic analysis of serotonin 5-HT1A receptors in the human brain, using [3H]8-OH-DPAT as a ligand, reveals region-specific decreases in receptor labeling with age in several cortical and hippocampal regions and in the raphe nuclei. Tritium 96-98 5-hydroxytryptamine receptor 1A Homo sapiens 52-58 1859823-6 1991 The 14 kDa protein labeled with [3H]7,7-azo-TC was radioimmunoprecipitated from cytosol by rabbit antiserum raised against purified I-BABP. Tritium 33-35 fatty acid binding protein 6 Rattus norvegicus 132-138 1310281-5 1992 When the latter cell population was coaggregated with purified gonadotrophs from 14-day-old rats, LHRH did enhance [3H]T labeling of lactotrophs and decreased that of somatotrophs. Tritium 116-118 gonadotropin releasing hormone 1 Rattus norvegicus 98-102 3759974-13 1986 We speculate that [3H] LacCer is taken up by FH homozygous cells via a LDL receptor-independent mechanism and accumulates in the cells without significant metabolism. Tritium 19-21 low density lipoprotein receptor Homo sapiens 71-83 1495270-4 1992 smg p21 GDS stimulates the dissociation of [3H]GDP from and also stimulates the binding of [35S]guanosine 5"-(3-O-thio)triphosphate (GTP gamma S) to smg p21s. Tritium 44-46 small nuclear ribonucleoprotein G Bos taurus 0-3 1325802-3 1992 Binding of [3H]DAGO to mu receptors in neocortex and the CA1 pyramidal layer of the hippocampus was significantly decreased in the 24-h group (p less than 0.05). Tritium 12-14 carbonic anhydrase 1 Rattus norvegicus 57-60 1323203-2 1992 This line exhibits a 95-fold increased Vmax for [3H]-MTX influx as compared to parental cells. Tritium 49-51 metaxin 1 Homo sapiens 53-56 1323203-3 1992 The values for [3H]-MTX influx Km, efflux t1/2 and structural specificity for other (anti)folate compounds were unchanged. Tritium 16-18 metaxin 1 Homo sapiens 20-23 1935836-7 1991 An inhibition of [3H]choline uptake and incorporation into phospholipids of fetal human lung explants and fetal lung type II pneumonocytes was induced by PAF. Tritium 18-20 PCNA clamp associated factor Homo sapiens 154-157 2032239-6 1991 Using a chimeric protein composed of IL-4 and Pseudomonas exotoxin (IL-4-PE40), we observed that IL-4-PE40 was cytotoxic (determined by inhibition of protein synthesis by [3H]leucine uptake) to MCA-106 tumor cells in a dose-dependent manner. Tritium 172-174 interleukin 4 Mus musculus 37-41 2032239-6 1991 Using a chimeric protein composed of IL-4 and Pseudomonas exotoxin (IL-4-PE40), we observed that IL-4-PE40 was cytotoxic (determined by inhibition of protein synthesis by [3H]leucine uptake) to MCA-106 tumor cells in a dose-dependent manner. Tritium 172-174 interleukin 4 Mus musculus 68-72 2032239-6 1991 Using a chimeric protein composed of IL-4 and Pseudomonas exotoxin (IL-4-PE40), we observed that IL-4-PE40 was cytotoxic (determined by inhibition of protein synthesis by [3H]leucine uptake) to MCA-106 tumor cells in a dose-dependent manner. Tritium 172-174 interleukin 4 Mus musculus 68-72 1870448-10 1991 Synthesis and secretion of PG-S1, -S2 and fibromodulin wa studied by labeling with [3H]-leucine and [35S]-sulfate. Tritium 84-86 phosphatidylglycerophosphate synthase 1 Homo sapiens 27-37 3020992-6 1986 Insulin and epidermal growth factor induced two- and threefold increases in [3H] thymidine incorporation into DNA, respectively. Tritium 77-79 epidermal growth factor Mus musculus 12-35 2051992-1 1991 Specific binding of tritium-labeled platelet-activating factor (PAF) and a nonmetabolizable bioactive analog of PAF, 1-O-alkyl-2-N-methylcarbamyl-sn-glyceryl-3-phosphorylcholine, to human platelet membranes was found to be potentiated by wheat germ agglutinin (WGA) and erythroagglutinin. Tritium 20-27 PCNA clamp associated factor Homo sapiens 36-62 2051992-1 1991 Specific binding of tritium-labeled platelet-activating factor (PAF) and a nonmetabolizable bioactive analog of PAF, 1-O-alkyl-2-N-methylcarbamyl-sn-glyceryl-3-phosphorylcholine, to human platelet membranes was found to be potentiated by wheat germ agglutinin (WGA) and erythroagglutinin. Tritium 20-27 PCNA clamp associated factor Homo sapiens 64-67 1346364-6 1992 As observed previously, prenatal ethanol exposure throughout gestation resulted in NMDA-sensitive [3H]-glutamate binding site reductions in the apical dendritic field regions of dentate gyrus, hippocampal CA1 and subiculum of dorsal hippocampal formation compared to the ad lib or pair-fed control groups. Tritium 99-101 carbonic anhydrase 1 Rattus norvegicus 205-208 1310882-5 1992 The growth plate chondrocytes, however, demonstrated a marked mitogenic response to PTH, with a 39-fold increase of [3H]-thymidine incorporation into DNA. Tritium 117-119 parathyroid hormone Gallus gallus 84-87 3024998-1 1986 The ontogenesis of delta-opioid receptors has been studied in the postnatal period up to day 50 using the highly selective delta-site ligand [3H][D-Pen2,D-Pen5]enkephalin ([3H]DPDPE) in binding studies. Tritium 142-144 proenkephalin Rattus norvegicus 160-170 1310882-7 1992 Furthermore, PTH depressed collagen synthesis as measured by [3H]-proline incorporation. Tritium 62-64 parathyroid hormone Gallus gallus 13-16 1733553-10 1992 The most marked reduction in [3H]-thymidine incorporation into SCC-25 cells occurred following treatment with the combination of beta-carotene and melphalan. Tritium 30-32 serpin family B member 3 Homo sapiens 63-66 1926229-2 1991 Scatchard plot analysis of the equilibrium binding data showed that [3H]NOM-Ac binds to uterine PgR with a Kd of 5.44 +/- 1.27 nM and a Bmax of 1.51 +/- 0.11 pmol/mg protein. Tritium 69-71 progesterone receptor Rattus norvegicus 96-99 1926229-3 1991 Analysis of dissociation kinetics showed that [3H]NOM-Ac dissociates slowly from the PgR, k - 1 = 4.9 +/- 0.5 10(-5) s-1. Tritium 47-49 progesterone receptor Rattus norvegicus 85-88 1653636-4 1991 The delta ligand, [3H]DPDPE [( D-Pen2.5]-enkephalin), bound with a Kd of 4.31 nM, and the kappa-ligand, [3H]U69593, bound with a Kd of 2.27 nM. Tritium 19-21 proenkephalin Rattus norvegicus 41-51 1733557-9 1992 The effect of DP in prolonging ara-CTP retention was proportional to the number of [3H]-NBMPR binding sites. Tritium 84-86 solute carrier family 25 member 1 Homo sapiens 35-38 3766957-1 1986 Photoaffinity labeling with [17 alpha-methyl-3H]promegestone ([ 3H]R5020) is an effective technique for the covalent labeling of the progesterone receptor (PR), which allows monitoring of the steroid receptor complex under denaturing conditions. Tritium 45-47 progesterone receptor Oryctolagus cuniculus 133-154 1344289-4 1992 Quantitation of [3H]-thymidine labeled cells demonstrated that laminin and fibronectin preadsorbed ceramics retain approximately double the number of marrow-derived mesenchymal cells than do untreated ceramics harvested 1 wk postimplantation. Tritium 17-19 fibronectin 1 Rattus norvegicus 75-86 1656983-2 1991 When rat pancreatic islets were incubated in the presence of unlabelled D-glucose (16.7 mM) and 3HOH, the production of 3H-labelled material susceptible to be phosphorylated by yeast hexokinase and then detritiated by yeast phosphoglucoisomerase did not exceed 2.66 +/- 0.21 pmol/islet per 180 min, i.e. about 1% of the rate of exogenous D-[5-3H]glucose utilization. Tritium 96-98 hexokinase Saccharomyces cerevisiae S288C 183-193 3766957-1 1986 Photoaffinity labeling with [17 alpha-methyl-3H]promegestone ([ 3H]R5020) is an effective technique for the covalent labeling of the progesterone receptor (PR), which allows monitoring of the steroid receptor complex under denaturing conditions. Tritium 45-47 progesterone receptor Oryctolagus cuniculus 156-158 3766957-4 1986 When [3H]R5020 alone was irradiated, there was a rapid (less than 5 min), light dependent destruction of [3H]R5020, as evident from increased formation of a more polar tritiated product on TLC and a concomitant decrease in the ability of the irradiated preparation to bind to PR. Tritium 6-8 progesterone receptor Oryctolagus cuniculus 276-278 1897971-4 1991 When equal concentrations of [3H]hexadecyllyso-GPC or [3H]hexadecyllyso-sn-glycero-3-phosphoethanolamine (-GPE) are incubated under identical conditions with myocytes for 4, 12, and 24 h, the rate of plasmenylcholine formation is faster from [3H]hexadecyllyso-GPE than from [3H]hexadecyllyso-GPC. Tritium 30-32 glycophorin C Rattus norvegicus 47-50 3766957-4 1986 When [3H]R5020 alone was irradiated, there was a rapid (less than 5 min), light dependent destruction of [3H]R5020, as evident from increased formation of a more polar tritiated product on TLC and a concomitant decrease in the ability of the irradiated preparation to bind to PR. Tritium 106-108 progesterone receptor Oryctolagus cuniculus 276-278 1544322-3 1992 In situ hybridization analyses using 3H-labeled cDNA and genomic DNA probes for the human type I cGK to human metaphase chromosomes supported the somatic cell hybrid data and indicated that the gene (PRKG1B; protein kinase, cGMP-dependent) maps to 10p11.2----q11.2. Tritium 37-39 protein kinase cGMP-dependent 1 Homo sapiens 97-100 1544322-3 1992 In situ hybridization analyses using 3H-labeled cDNA and genomic DNA probes for the human type I cGK to human metaphase chromosomes supported the somatic cell hybrid data and indicated that the gene (PRKG1B; protein kinase, cGMP-dependent) maps to 10p11.2----q11.2. Tritium 37-39 protein kinase cGMP-dependent 1 Homo sapiens 200-206 1337056-5 1992 IGF-II led to dose-dependent increases in [3H]thymidine incorporation into DNA and in the number of granulosa cells. Tritium 43-45 insulin like growth factor 2 Homo sapiens 0-6 1320624-3 1992 NPY, its natural analogues and the Y1-receptor-specific peptide ligand [Leu31,Pro34]NPY inhibited [3H]NPY binding in the potency order: [Leu31,Pro34]NPY greater than or equal to human NPY greater than or equal to peptide YY (PYY) greater than salmon pancreatic polypeptide (PP) greater than human PP greater than porcine NPY13-36 much greater than NPY22-36. Tritium 99-101 neuropeptide Y Homo sapiens 84-87 1713871-6 1991 Stimulation of alpha-1 adrenergic receptors with phenylephrine (10(-6) mol.l-1) was accompanied with only slight but significant increase in (3H)DHA binding and with a significant reduction in the total number of Ca2+ channel agonist binding sites. Tritium 142-144 adrenoceptor alpha 1D Homo sapiens 15-22 1900462-2 1991 In the conditioned medium (CM) of ATL-2, we found an inhibitory activity on the epidermal growth factor (EGF)-dependent proliferation of primary cultured rat hepatocytes, measured by cell number and [3H]thymidine incorporation. Tritium 200-202 atlastin GTPase 2 Rattus norvegicus 34-39 2875589-0 1986 Assay for thyroid growth stimulating immunoglobulins: stimulation of [3H]thymidine incorporation into isolated thyroid follicles by TSH, EGF, and immunoglobulins from goitrous patients in an iodine-deficient region. Tritium 70-72 epidermal growth factor Homo sapiens 137-140 1995287-5 1991 When the antiprogestin [3H]RU486 was used instead of [3H]R5020 as a ligand, a 9 S PR was also found in the cytosol, but a nonactivated "8.5 S" receptor complex was identified in the high salt nuclear fraction in presence of tungstate ions. Tritium 24-26 progesterone receptor Rattus norvegicus 82-84 1995287-5 1991 When the antiprogestin [3H]RU486 was used instead of [3H]R5020 as a ligand, a 9 S PR was also found in the cytosol, but a nonactivated "8.5 S" receptor complex was identified in the high salt nuclear fraction in presence of tungstate ions. Tritium 54-56 progesterone receptor Rattus norvegicus 82-84 1469900-7 1992 [3H]-thymidine labeling index was dramatically increased in the entire epithelium in dysplasia, and also increased in cell layer 3 of MBS, BH1 and D. Tritium 1-3 MBS1 Homo sapiens 134-148 2428509-2 1986 Activation of the T-cell lines with guinea pig myelin basic protein (GP-BP) presented by accessory cells (APC) resulted in an increase in the number of blast cells in culture and was reflected by increased uptake of [3H]thymidine [( 3H]Tdy). Tritium 217-219 myelin basic protein Cavia porcellus 47-67 1672374-3 1991 Desipramine, imipramine, amitriptyline and iprindole inhibited thrombin-stimulated formation of [3H]IP2 and [3H]IP3 in human platelets but had no significant effect on [3H]IP1 formation. Tritium 97-99 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 100-103 1847192-2 1991 Polyacrylamide gel electrophoresis in sodium dodecyl sulfate (SDS-PAGE) of codfish GABA receptor photoaffinity-labeled by both [3H]flunitrazepam ([3H]Flu) and [3H]muscimol showed a single radioactive peak with molecular mass of 56 kDa, in contrast to the multiple subunits found in other vertebrate species. Tritium 128-130 GABA type A receptor-associated protein Homo sapiens 83-96 1988553-8 1991 In conclusion, we suggest that [3H]harman binding is a biochemical tool as a selective marker to quantify MAO-A in the CNS of different mammalian species as well as in extraneuronal tissues. Tritium 32-34 monoamine oxidase A Homo sapiens 106-111 2428509-2 1986 Activation of the T-cell lines with guinea pig myelin basic protein (GP-BP) presented by accessory cells (APC) resulted in an increase in the number of blast cells in culture and was reflected by increased uptake of [3H]thymidine [( 3H]Tdy). Tritium 233-235 myelin basic protein Cavia porcellus 47-67 1596583-2 1992 In various tumor cell lines and clinical tumor biopsies, TS mRNA levels correlated with TS levels as determined by [3H]-fluorodeoxyuridylate binding. Tritium 116-118 thymidylate synthetase Homo sapiens 57-59 1596583-2 1992 In various tumor cell lines and clinical tumor biopsies, TS mRNA levels correlated with TS levels as determined by [3H]-fluorodeoxyuridylate binding. Tritium 116-118 thymidylate synthetase Homo sapiens 88-90 3522207-12 1986 Incorporation of [3H]GlcN into intracellular combined LH alpha, LH beta, and free alpha was increased with GnRH in both intacts and castrates, such that the 3H to 35S ratio, reflecting relative glycosylation, was also increased with GnRH. Tritium 18-20 gonadotropin releasing hormone 1 Rattus norvegicus 107-111 1898734-2 1991 The region(s) of bovine galactosyltransferase that interacts with the lactose synthase regulatory protein alpha-lactalbumin was investigated using trace 3H acetylation to probe the effects of alpha-lactalbumin on the reactivities of the individual amino groups of galactosyltransferase. Tritium 153-155 lactalbumin alpha Bos taurus 106-123 2069325-5 1991 In addition, [3H]TdR suicide experiments indicate that TGF-beta 2 blocks the IL-3-induced progression of early progenitors into the S phase of the cell cycle, whereas IL-6 and bFGF potentiate their entry into the mitotic process. Tritium 14-16 interleukin 3 Homo sapiens 77-81 1687682-7 1991 4-AP, 3,4-diaminopyridine (3,4-DAP), TEA and the four dendrotoxins displaced the binding of [3H]p-aminoclonidine ([3H]PAC) from alpha 2-receptors. Tritium 93-95 death-associated protein Rattus norvegicus 31-34 2425365-0 1986 Discrimination of muscle and neuronal Na-channel subtypes by binding competition between [3H]saxitoxin and mu-conotoxins. Tritium 90-92 sodium voltage-gated channel alpha subunit 8 Rattus norvegicus 38-48 1726089-5 1991 Conversely, the selective NK1 receptor agonist, [Sar9,Met(O2)11]substance P, reproduced the biphasic dose-response curve for [3H]protein secretion typical of native substance P. However, a biphasic response was not observed with peptides deprived of the N-terminal moiety of substance P, such as substance P-(4-11) or [AcArg6,Sar9,Met(O2)11] substance P-(6-11). Tritium 126-128 tachykinin receptor 1 Rattus norvegicus 26-38 1932206-6 1991 Two nuclear matrix proteins, p52 and p55, incorporated [3H] retinoic acid rapidly, were cell-cycle-related, and disappeared within 12 h of dosing. Tritium 56-58 H3 histone pseudogene 44 Homo sapiens 37-40 3719375-6 1986 [3H]5-HT was used to label both 5-HT1A and 5-HT1B receptor subtypes. Tritium 1-3 5-hydroxytryptamine receptor 1A Homo sapiens 32-38 1913239-2 1991 Quantitative autoradiographic studies on rat and guinea pig brain sections incubated with 2 nM [3H]5-HT showed a preferential affinity of S-CM-GTNH2 for 5-HT1B and 5-HT1D sites. Tritium 96-98 5-hydroxytryptamine receptor 1D Cavia porcellus 164-170 1667606-3 1991 [3H]Rauwolscine, besides binding to alpha 2 adrenoceptors, also binds to 5-HT1A receptors. Tritium 1-3 5-hydroxytryptamine receptor 1A Homo sapiens 73-79 1667606-8 1991 The regional distribution of 5-HT1A receptors labelled by [3H]rauwolscine is in agreement with previous studies using [3H]8-OH-DPAT. Tritium 59-61 5-hydroxytryptamine receptor 1A Homo sapiens 29-35 3697993-2 1986 The growth factors, particularly EGF and FGF, increased DNA synthesis in a dose-dependent manner as measured by [3H]thymidine incorporation, and they stimulated submaximal cell proliferation. Tritium 113-115 epidermal growth factor Homo sapiens 33-36 1667606-8 1991 The regional distribution of 5-HT1A receptors labelled by [3H]rauwolscine is in agreement with previous studies using [3H]8-OH-DPAT. Tritium 119-121 5-hydroxytryptamine receptor 1A Homo sapiens 29-35 1817966-0 1991 [3H]5-carboxamidotryptamine labels multiple high affinity 5-HT1D-like sites in guinea pig brain. Tritium 1-4 5-hydroxytryptamine receptor 1D Cavia porcellus 58-64 1720092-8 1991 coli or hIGFBP-3CHO potentiated subsequent IGF-I-stimulated [3H]AIB uptake. Tritium 61-63 insulin like growth factor binding protein 3 Homo sapiens 8-16 1837466-6 1991 Loss of proliferative potential, in response to GM-CSF, was confirmed using [3H]-thymidine incorporation. Tritium 77-79 colony stimulating factor 2 Homo sapiens 48-54 1708055-7 1991 The calcium antagonist properties of AHR-16303B were demonstrated by inhibition of (a) [3H]nimodipine binding to voltage-sensitive calcium channels on rabbit skeletal muscle membranes (IC50 = 15 nM), (b) KCl-stimulated calcium flux into cultured PC12 cells (IC50 = 81 nM), and (c) CaCl2-induced contractions of rabbit thoracic aortic strips (pA2 = 8.84). Tritium 88-90 aryl hydrocarbon receptor Oryctolagus cuniculus 37-40 2031710-0 1991 Post- and presynaptic lesions in the CA1 region of hippocampus: effect on [3H]forskolin and [3H]phorboldibutyrate ester binding. Tritium 75-77 carbonic anhydrase 1 Rattus norvegicus 37-40 2031710-0 1991 Post- and presynaptic lesions in the CA1 region of hippocampus: effect on [3H]forskolin and [3H]phorboldibutyrate ester binding. Tritium 93-95 carbonic anhydrase 1 Rattus norvegicus 37-40 2031710-2 1991 Four days after transient four vessel occlusion a 80% loss of [3H]FOR and a 35% loss of [3H]PDBU binding could be measured in the CA1 stratum radiatum of operated Wistar rats as compared to control rats. Tritium 89-91 carbonic anhydrase 1 Rattus norvegicus 130-133 1958660-5 1991 Modified enzyme, prepared by incubating glutathione S-transferase with [3H]S-BDB-G in the absence or in the presence of S-hexylglutathione, was reduced with NaBH4, carboxymethylated, and digested with trypsin. Tritium 72-74 hematopoietic prostaglandin D synthase Rattus norvegicus 40-65 3700016-7 1986 Biochemical studies indicated that the 3H-label was present as an integral membrane component in the rod OS and confirmed that canine rhodopsin is a fucosylated glycoprotein. Tritium 39-41 rhodopsin Canis lupus familiaris 134-143 1676067-2 1991 8-MOP (1 microgram mL-1) increased [3H]TdR incorporation and caused a rise in the blastic and mitotic index in all experimental cultures. Tritium 36-38 L1 cell adhesion molecule Mus musculus 19-23 3009492-1 1986 Human insulin-like growth factor I and II (IGF-I and IGF-II) in concentrations of 1-30 ng/ml, were shown to stimulate ornithine decarboxylase activity and [3H]thymidine incorporation in human SH-SY5Y neuroblastoma cells. Tritium 156-158 insulin like growth factor 2 Homo sapiens 53-59 1705306-6 1991 In short-term liquid cultures, IL-3, GM-CSF and G-CSF increased 3H-thymidine incorporation. Tritium 64-66 interleukin 3 Homo sapiens 31-35 1705306-6 1991 In short-term liquid cultures, IL-3, GM-CSF and G-CSF increased 3H-thymidine incorporation. Tritium 64-66 colony stimulating factor 2 Homo sapiens 37-43 1705306-6 1991 In short-term liquid cultures, IL-3, GM-CSF and G-CSF increased 3H-thymidine incorporation. Tritium 64-66 colony stimulating factor 3 Homo sapiens 48-53 1659411-2 1991 [3H]PAF binding to these cells was time-dependent, reaching equilibrium at 60 minutes, and saturable. Tritium 1-3 PCNA clamp associated factor Homo sapiens 4-7 1662554-6 1991 Both TGF-beta and epidermal growth factor (EGF) stimulated [3H]-thymidine incorporation into cells. Tritium 60-62 epidermal growth factor Mus musculus 18-41 3011453-5 1986 Adenosine, L-PIA and NECA but not D-PIA inhibited [3H]NA release during nerve stimulation. Tritium 51-53 RPTOR independent companion of MTOR complex 2 Homo sapiens 13-16 1941621-3 1991 When quiescent cells were stimulated with platelet-derived growth factor followed by insulin, HA1077 (1-30 microM), administered together with either stimulation, showed dose-dependent inhibition of [3H]thymidine incorporation. Tritium 200-202 insulin Bos taurus 85-92 2064786-0 1991 [3H]-13-cis-retinoic acid covalently binds to thioredoxin reductase in human keratinocytes. Tritium 1-3 peroxiredoxin 5 Homo sapiens 46-67 2064786-2 1991 [3H]-labeled 13-cis-retinoic acid has been used to covalently label thioredoxin reductase in human keratinocytes. Tritium 1-3 peroxiredoxin 5 Homo sapiens 68-89 3011168-2 1986 Apart from GABA itself, GABA A-receptor agonists inhibited the CA1 population spikes with potencies that correlated closely (r = 0.96) with their ability to displace [3H]-GABA from GABAA-binding sites. Tritium 167-169 carbonic anhydrase 1 Rattus norvegicus 63-66 1657195-1 1991 The stable [3H]prostaglandin E1 (PGE1)-bound receptor, which couples to 60 kDa GTP-binding protein, from membranes of mouse mastocytoma P-815 cells has been purified and characterized. Tritium 12-14 RAB2A, member RAS oncogene family Mus musculus 79-98 2278838-4 1990 We characterized AR binding using [3H]R1881 as the ligand in cytosols from tissues obtained on days 100-138 of gestation. Tritium 35-37 androgen receptor Macaca mulatta 17-19 3007537-10 1986 After 24 hours of EGF treatment, DNA synthesis is reduced to less than 10% compared to untreated controls as measured by the incorporation of [3H]thymidine or BrdU. Tritium 143-145 epidermal growth factor Homo sapiens 18-21 2281603-9 1990 ASFV-infection of P-MM, placed in culture medium with CSF-1, caused a pronounced transient increase in total 3H-TdR incorporation at the early onset of CPE and HAd. Tritium 109-111 colony stimulating factor 1 Homo sapiens 54-59 1840455-2 1991 Experiments in vitro showed inhibitory effects on [3H]TdR incorporation during human peripheral T- and B-lymphocyte proliferation by SY 0.03-3.0, 0.1-2.0 mg.ml-1 respectively, murine mixed lymphocyte culture response and the production of interleukin-2 by SY 0.1-2.5 mg.ml-1. Tritium 51-53 interleukin 2 Mus musculus 239-252 3753716-4 1986 The specificity of the CaBP mRNA-DNA hybrid formation was checked using 3H-labeled plasmid pBR322 DNA as a control probe. Tritium 72-74 S100 calcium binding protein G Rattus norvegicus 23-27 1769103-4 1991 An examination of [3H]thymidine incorporation by glial fibrillary acidic protein-(GFAP) immunoreactive astrocytes with flat (type 1) morphology in dissociated cultures derived from postnatal day 1 mice revealed that the reduction in glial numbers at 3 DIV was not immediately preceded by a reduction in the rate of [3H]thymidine incorporation at 2 DIV, although previous studies have shown that opioids inhibit the rate of [3H]thymidine incorporation by more mature astrocytes at 4 or 6 DIV. Tritium 19-21 glial fibrillary acidic protein Mus musculus 82-86 1769103-4 1991 An examination of [3H]thymidine incorporation by glial fibrillary acidic protein-(GFAP) immunoreactive astrocytes with flat (type 1) morphology in dissociated cultures derived from postnatal day 1 mice revealed that the reduction in glial numbers at 3 DIV was not immediately preceded by a reduction in the rate of [3H]thymidine incorporation at 2 DIV, although previous studies have shown that opioids inhibit the rate of [3H]thymidine incorporation by more mature astrocytes at 4 or 6 DIV. Tritium 316-318 glial fibrillary acidic protein Mus musculus 82-86 2281603-11 1990 For P-MM that were cultured without CSF-1 and for MM-CSF, that were kept in culture with CSF-1, transient increases in 3H-TdR incorporation at the onset of CPE and HAd by ASFV-infection were evident, but were much less pronounced. Tritium 119-121 colony stimulating factor 1 Homo sapiens 89-94 1705154-1 1990 The ligand selectivity profile reveals that substance P (SP) and unlabeled [Sar9,Met(O2)11]-SP are potent competitors of [3H]-[Sar9,Met(O2)11]-SP binding while NK-2 and NK-3 analogues are virtually inactive demonstrating the selectivity of this radioligand for the NK-1 receptor class. Tritium 122-124 tachykinin receptor 1 Rattus norvegicus 265-278 1705154-5 1990 This could be explained by the existence of NK-1 receptor subtypes, only one of them being recognized by [3H]-[Sar9,Met(O2)11]-SP or by the greater selectivity of this radioligand for NK-1 over NK-2 and NK-3 receptor classes. Tritium 106-108 tachykinin receptor 1 Rattus norvegicus 44-57 2211721-6 1990 The [35S]cysteine and [3H]myristate-labeled p69 preparations were resolved by two-dimensional gel isoelectric focusing with pI values in the pH range of 7.0-8.0 and 7.0-7.5, respectively. Tritium 23-25 islet cell autoantigen 1 Homo sapiens 44-47 1769103-4 1991 An examination of [3H]thymidine incorporation by glial fibrillary acidic protein-(GFAP) immunoreactive astrocytes with flat (type 1) morphology in dissociated cultures derived from postnatal day 1 mice revealed that the reduction in glial numbers at 3 DIV was not immediately preceded by a reduction in the rate of [3H]thymidine incorporation at 2 DIV, although previous studies have shown that opioids inhibit the rate of [3H]thymidine incorporation by more mature astrocytes at 4 or 6 DIV. Tritium 316-318 glial fibrillary acidic protein Mus musculus 82-86 1769103-6 1991 The rate of [3H]thymidine incorporation by GFAP-immunoreactive astrocytes with process-bearing (type 2) morphology was unchanged by opioid treatment. Tritium 13-15 glial fibrillary acidic protein Mus musculus 43-47 1931944-11 1991 Since Glu-185 has been previously identified as a residue at the active site of smooth myosin using [3H]UDP as a photolabel [Garabedian, T. E., & Yount, R. G. (1990) J. Biol. Tritium 101-103 myosin heavy chain 14 Homo sapiens 87-93 1717146-9 1991 Moreover, the addition of mAbs against TGF alpha and EGFR but not EGF inhibited [3H]thymidine incorporation dose dependently in EGFR(+) cancer cells. Tritium 81-83 epidermal growth factor Homo sapiens 53-56 1963597-3 1990 The same treatment schedule with GnRH produced an increase in the number of [3H]imipramine Bmax in cortical membranes that was statistically significant at the dose of 2 micrograms/kg. Tritium 77-79 gonadotropin releasing hormone 1 Mus musculus 33-37 2213830-4 1990 Whereas tryptamine derivatives generally display little selectivity for the various populations of 5-HT receptors, N1-n-propyl-5-methoxy-alpha-methyltryptamine (3h) binds with significant affinity (Ki = 12 nM) and selectivity at 5-HT2 receptors relative to 5-HT1A (Ki = 7100 nM), 5-HT1B (Ki = 5000 nM), 5-HT1C (Ki = 120 nM), and 5-HT1D (Ki greater than 10,000 nM) receptors. Tritium 161-163 5-hydroxytryptamine receptor 1A Homo sapiens 257-263 3945071-7 1986 This immunotoxin 44X14-RTA was assayed for protein-synthesis inhibition in HT-29 colon carcinoma and HT-1080 sarcoma cells by [3H]leucine uptake. Tritium 127-129 MAS related GPR family member F Homo sapiens 23-26 2233691-8 1990 The hydroxy metabolites and their hydroperoxy precursors produced a 2- to 4-fold potentiation of EGF-stimulated [3H]thymidine incorporation in BALB/c 3T3 cells. Tritium 113-115 epidermal growth factor Mus musculus 97-100 1706487-4 1990 Radioactivity of the [3H] methionine residue of MBP of Twi was also similar to that of the controls. Tritium 22-24 galactosylceramidase Mus musculus 55-58 1936188-3 1991 Treatment of bovine rod outer segments (ROS) with galactose oxidase followed by reduction with tritium-labeled sodium borohydride revealed the presence of existing molecules of galactose on rhodopsin. Tritium 95-102 rhodopsin Bos taurus 190-199 1936188-6 1991 During the first 6 hr, galactose selectively labeled rhodopsin in the Golgi-enriched fraction resulting in increased [3H]/[14C] ratios in both Golgi and ROS. Tritium 118-120 rhodopsin Rattus norvegicus 53-62 3005828-6 1986 However, IAP treatment decreased [3H]angiotensin II binding affinity when studies were performed in the absence but not the presence of 5"-guanylylimidodiphosphate (GppNHp). Tritium 34-36 magnesium transporter 1 Rattus norvegicus 9-12 1660817-0 1991 Differences in the binding of [3H][D-Ser2,Thr6]leucine-enkephalin and [3H][D-Pen2,D-Pen5]enkephalin to brain membranes of morphine tolerant-dependent rats. Tritium 31-33 proenkephalin Rattus norvegicus 55-65 2253703-10 1990 Phospholipase A2 decreased both [3H](-)QNB and [3H]Oxo-M specific binding, the latter being affected to a greater extent. Tritium 33-35 LOC104974671 Bos taurus 0-16 2253703-10 1990 Phospholipase A2 decreased both [3H](-)QNB and [3H]Oxo-M specific binding, the latter being affected to a greater extent. Tritium 48-50 LOC104974671 Bos taurus 0-16 3010168-1 1986 The ability of N,N-diallyl-Tyr-Aib-Aib-Phe-Leu-OH (ICI 174864) to displace [3H]-[D-Ala2, D-Leu5]enkephalin bound to the delta-opioid site is increased 8-16 fold by addition of 25mM NaCl. Tritium 76-78 tripartite motif containing 13 Homo sapiens 91-95 3456171-2 1986 Tryptic digestion of DBI generates an octadecaneuropeptide (ODN) that is more potent than the parent compound in the displacement of specifically bound beta-[3H]carboline-3-carboxylate methyl ester [( 3H]BCCM) and in proconflict action (Vogel test in thirsty rats). Tritium 158-160 diazepam binding inhibitor Rattus norvegicus 21-24 2386935-6 1990 Under these conditions, expression of ASGP-1 is depressed by at least 50% in both cell lines, as monitored by [3H]glucosamine incorporation and by binding of peanut agglutinin to intact cells. Tritium 111-113 mucin 4, cell surface associated Rattus norvegicus 38-44 2167218-5 1990 The high basal activity in [3H]thymidine incorporation into DNA in aged cells is due to an increased responsiveness to insulin, insulin-like growth factor-I (IGF-I), or serum. Tritium 28-30 insulin-like growth factor 1 Rattus norvegicus 128-156 2167218-5 1990 The high basal activity in [3H]thymidine incorporation into DNA in aged cells is due to an increased responsiveness to insulin, insulin-like growth factor-I (IGF-I), or serum. Tritium 28-30 insulin-like growth factor 1 Rattus norvegicus 158-163 1653524-1 1991 Preincubation of human neutrophils with the human hormone granulocyte-macrophage colony-stimulating factor (GM-CSF) inhibits the specific binding of leukotriene B4 ([3H]LTB4) but not the nonmetabolizable bioactive platelet-activating factor ([3H]C-PAF) to intact cells. Tritium 166-168 colony stimulating factor 2 Homo sapiens 58-106 1653524-1 1991 Preincubation of human neutrophils with the human hormone granulocyte-macrophage colony-stimulating factor (GM-CSF) inhibits the specific binding of leukotriene B4 ([3H]LTB4) but not the nonmetabolizable bioactive platelet-activating factor ([3H]C-PAF) to intact cells. Tritium 166-168 colony stimulating factor 2 Homo sapiens 108-114 1653524-1 1991 Preincubation of human neutrophils with the human hormone granulocyte-macrophage colony-stimulating factor (GM-CSF) inhibits the specific binding of leukotriene B4 ([3H]LTB4) but not the nonmetabolizable bioactive platelet-activating factor ([3H]C-PAF) to intact cells. Tritium 243-245 colony stimulating factor 2 Homo sapiens 58-106 1653524-1 1991 Preincubation of human neutrophils with the human hormone granulocyte-macrophage colony-stimulating factor (GM-CSF) inhibits the specific binding of leukotriene B4 ([3H]LTB4) but not the nonmetabolizable bioactive platelet-activating factor ([3H]C-PAF) to intact cells. Tritium 243-245 colony stimulating factor 2 Homo sapiens 108-114 1833413-6 1991 3H-thymidine cell suicide studies suggest that higher percentages of pluripotent precursors from the bone marrow of the TcR gamma 4 transgenic mice were in the S phase of the cell cycle. Tritium 0-2 T cell receptor alpha variable 6-3 Mus musculus 120-123 2167218-6 1990 Thus, removal of serum and insulin from the medium eliminates the high basal [3H]thymidine incorporation into DNA, and this activity is restored by insulin or IGF-I in a concentration-dependent manner. Tritium 78-80 insulin-like growth factor 1 Rattus norvegicus 159-164 2384761-4 1990 Using equilibrium binding studies, [3H]proprionyl-NPY was found to bind in both tissues with high affinity to a single class of receptors with a similar KD (0.035 nM). Tritium 36-38 neuropeptide Y Homo sapiens 50-53 2384761-6 1990 Competition binding experiments showed that peptide YY (PYY) was equal to NPY in its ability to displace [3H]proprionyl-NPY, whereas rat and human pancreatic polypeptide were without effect up to a concentration of 10(-6) M. This suggests that, whereas PYY and NPY may compete for the same receptor(s), the pancreatic polypeptides probably act on a separate population of receptors. Tritium 106-108 neuropeptide Y Homo sapiens 120-123 2198284-7 1990 [Acetyl-3H]O-acetyl groups were found at both the 7- and the 9-positions, indicating that both 7-O-acetyl GD3 and 9-O-acetyl GD3 were synthesized by the action of O-acetyltransferase(s) on endogenous GD3. Tritium 8-10 GRDX Homo sapiens 106-109 2198284-7 1990 [Acetyl-3H]O-acetyl groups were found at both the 7- and the 9-positions, indicating that both 7-O-acetyl GD3 and 9-O-acetyl GD3 were synthesized by the action of O-acetyltransferase(s) on endogenous GD3. Tritium 8-10 GRDX Homo sapiens 125-128 1653834-1 1991 [D-Pen2,4"-125I-Phe4,D-Pen5]enkephalin ([125I]DPDPE) is a highly selective radioligand for the delta opioid receptor with a specific activity (2200 Ci/mmol) that is over 50-fold greater than that of tritium-labeled DPDPE analogs. Tritium 199-206 proenkephalin Rattus norvegicus 28-38 1678315-10 1991 Although IGF-II caused a significant increase in [3H]thymidine incorporation in both undifferentiated and differentiated Tera-2 cells, the magnitude of the response and the sensitivity of the cells to IGF-II stimulation was diminished following differentiation. Tritium 50-52 insulin like growth factor 2 Homo sapiens 9-15 1678315-10 1991 Although IGF-II caused a significant increase in [3H]thymidine incorporation in both undifferentiated and differentiated Tera-2 cells, the magnitude of the response and the sensitivity of the cells to IGF-II stimulation was diminished following differentiation. Tritium 50-52 insulin like growth factor 2 Homo sapiens 201-207 2198284-7 1990 [Acetyl-3H]O-acetyl groups were found at both the 7- and the 9-positions, indicating that both 7-O-acetyl GD3 and 9-O-acetyl GD3 were synthesized by the action of O-acetyltransferase(s) on endogenous GD3. Tritium 8-10 GRDX Homo sapiens 125-128 3456171-9 1986 In primary cultures of cerebellar granule cells of rat, DBI, ODN, octapeptide, heptapeptide, and hexapeptide preferentially displace [3H]BCCM over [3H]flunitrazepam; moreover, they displace bound [3H]BCCM completely but [3H]flunitrazepam only by 50%. Tritium 134-136 diazepam binding inhibitor Rattus norvegicus 56-59 2198284-9 1990 Surprisingly, the major 3H-labeled product of the in vitro labeling reaction was not O-acetyl-GD3, but GD3, with the label exclusively in the sialic acid residues. Tritium 24-26 GRDX Homo sapiens 103-106 2198284-12 1990 Pulse-chase analysis of the 3H/14C ratio showed that the N-acetyl groups of both GD3 and of the monosialoganglioside GM3 were turning over faster than the parent molecules. Tritium 28-30 GRDX Homo sapiens 81-84 1714275-4 1991 Gastrin (HG-17), CCK-39 and CCK-8 induced [3H]-inositol phosphate ([3H]InsP) accumulation from [3H]inositol prelabelled cells with a high potency (EC50: 0.3-2.7 nM) but CCK-8 exhibited a higher efficacy than HG-17 or CCK-39. Tritium 68-70 cholecystokinin Oryctolagus cuniculus 17-20 1714275-4 1991 Gastrin (HG-17), CCK-39 and CCK-8 induced [3H]-inositol phosphate ([3H]InsP) accumulation from [3H]inositol prelabelled cells with a high potency (EC50: 0.3-2.7 nM) but CCK-8 exhibited a higher efficacy than HG-17 or CCK-39. Tritium 68-70 cholecystokinin Oryctolagus cuniculus 28-31 3456171-9 1986 In primary cultures of cerebellar granule cells of rat, DBI, ODN, octapeptide, heptapeptide, and hexapeptide preferentially displace [3H]BCCM over [3H]flunitrazepam; moreover, they displace bound [3H]BCCM completely but [3H]flunitrazepam only by 50%. Tritium 148-150 diazepam binding inhibitor Rattus norvegicus 56-59 1714275-4 1991 Gastrin (HG-17), CCK-39 and CCK-8 induced [3H]-inositol phosphate ([3H]InsP) accumulation from [3H]inositol prelabelled cells with a high potency (EC50: 0.3-2.7 nM) but CCK-8 exhibited a higher efficacy than HG-17 or CCK-39. Tritium 68-70 cholecystokinin Oryctolagus cuniculus 28-31 3456171-9 1986 In primary cultures of cerebellar granule cells of rat, DBI, ODN, octapeptide, heptapeptide, and hexapeptide preferentially displace [3H]BCCM over [3H]flunitrazepam; moreover, they displace bound [3H]BCCM completely but [3H]flunitrazepam only by 50%. Tritium 148-150 diazepam binding inhibitor Rattus norvegicus 56-59 1714275-4 1991 Gastrin (HG-17), CCK-39 and CCK-8 induced [3H]-inositol phosphate ([3H]InsP) accumulation from [3H]inositol prelabelled cells with a high potency (EC50: 0.3-2.7 nM) but CCK-8 exhibited a higher efficacy than HG-17 or CCK-39. Tritium 68-70 cholecystokinin Oryctolagus cuniculus 28-31 1714275-4 1991 Gastrin (HG-17), CCK-39 and CCK-8 induced [3H]-inositol phosphate ([3H]InsP) accumulation from [3H]inositol prelabelled cells with a high potency (EC50: 0.3-2.7 nM) but CCK-8 exhibited a higher efficacy than HG-17 or CCK-39. Tritium 68-70 cholecystokinin Oryctolagus cuniculus 17-20 2228798-8 1990 Thirty nM insulin stimulated the incorporation of [3H]-leucine into protein of the organ of Corti from 39 to 56 pmol/mg protein but was ineffective in the tissues of the lateral wall. Tritium 51-53 insulin Bos taurus 10-17 3456171-9 1986 In primary cultures of cerebellar granule cells of rat, DBI, ODN, octapeptide, heptapeptide, and hexapeptide preferentially displace [3H]BCCM over [3H]flunitrazepam; moreover, they displace bound [3H]BCCM completely but [3H]flunitrazepam only by 50%. Tritium 148-150 diazepam binding inhibitor Rattus norvegicus 56-59 1714275-4 1991 Gastrin (HG-17), CCK-39 and CCK-8 induced [3H]-inositol phosphate ([3H]InsP) accumulation from [3H]inositol prelabelled cells with a high potency (EC50: 0.3-2.7 nM) but CCK-8 exhibited a higher efficacy than HG-17 or CCK-39. Tritium 68-70 cholecystokinin Oryctolagus cuniculus 28-31 1714275-4 1991 Gastrin (HG-17), CCK-39 and CCK-8 induced [3H]-inositol phosphate ([3H]InsP) accumulation from [3H]inositol prelabelled cells with a high potency (EC50: 0.3-2.7 nM) but CCK-8 exhibited a higher efficacy than HG-17 or CCK-39. Tritium 68-70 cholecystokinin Oryctolagus cuniculus 28-31 2164573-6 1990 Scatchard analysis of binding of the mu-specific ligand [3H]D-Ala2,N-Me-Phe4,Gly-ol5-enkephalin to embryonic striatal cell membranes revealed an apparent single class of sites with an affinity (KD) of 0.4 +/- 0.1 nM and a density (Bmax) of 160 +/- 20 fmol/mg of protein. Tritium 57-59 proenkephalin Rattus norvegicus 85-95 3941067-4 1986 259, 2697-2700) suggested that nuclear type II sites in the rat uterus are of eosinophil origin and may represent [3H]estradiol binding to eosinophil peroxidase. Tritium 115-117 eosinophil peroxidase Rattus norvegicus 139-160 2123983-5 1990 The 3H-AFB1-metabolite-binding to the nasal glands in vitro was inhibited by the cytochrome P-450-inhibitor metyrapone and by CO- and N2-atmospheres indicating a cytochrome P-450-dependent bioactivation of the AFB1 in these glands. Tritium 4-6 cytochrome P450, family 21, subfamily a, polypeptide 1 Mus musculus 81-97 1714275-4 1991 Gastrin (HG-17), CCK-39 and CCK-8 induced [3H]-inositol phosphate ([3H]InsP) accumulation from [3H]inositol prelabelled cells with a high potency (EC50: 0.3-2.7 nM) but CCK-8 exhibited a higher efficacy than HG-17 or CCK-39. Tritium 68-70 cholecystokinin Oryctolagus cuniculus 28-31 1714275-5 1991 HG-17, CCK-8 and CCK-39 induced a rapid accumulation of [3H]inositol monophosphate ([3H]InsP1), [3H]inositol bisphosphate ([3H]InsP2) and [3H]inositol trisphosphate ([3H]InsP3) but CCK-8 caused a two times higher accumulation than HG-17 or CCK-39. Tritium 57-59 cholecystokinin Oryctolagus cuniculus 7-10 1714275-5 1991 HG-17, CCK-8 and CCK-39 induced a rapid accumulation of [3H]inositol monophosphate ([3H]InsP1), [3H]inositol bisphosphate ([3H]InsP2) and [3H]inositol trisphosphate ([3H]InsP3) but CCK-8 caused a two times higher accumulation than HG-17 or CCK-39. Tritium 57-59 cholecystokinin Oryctolagus cuniculus 17-20 1714275-5 1991 HG-17, CCK-8 and CCK-39 induced a rapid accumulation of [3H]inositol monophosphate ([3H]InsP1), [3H]inositol bisphosphate ([3H]InsP2) and [3H]inositol trisphosphate ([3H]InsP3) but CCK-8 caused a two times higher accumulation than HG-17 or CCK-39. Tritium 57-59 cholecystokinin Oryctolagus cuniculus 17-20 1714275-5 1991 HG-17, CCK-8 and CCK-39 induced a rapid accumulation of [3H]inositol monophosphate ([3H]InsP1), [3H]inositol bisphosphate ([3H]InsP2) and [3H]inositol trisphosphate ([3H]InsP3) but CCK-8 caused a two times higher accumulation than HG-17 or CCK-39. Tritium 57-59 cholecystokinin Oryctolagus cuniculus 17-20 1714275-5 1991 HG-17, CCK-8 and CCK-39 induced a rapid accumulation of [3H]inositol monophosphate ([3H]InsP1), [3H]inositol bisphosphate ([3H]InsP2) and [3H]inositol trisphosphate ([3H]InsP3) but CCK-8 caused a two times higher accumulation than HG-17 or CCK-39. Tritium 85-87 cholecystokinin Oryctolagus cuniculus 7-10 1714275-5 1991 HG-17, CCK-8 and CCK-39 induced a rapid accumulation of [3H]inositol monophosphate ([3H]InsP1), [3H]inositol bisphosphate ([3H]InsP2) and [3H]inositol trisphosphate ([3H]InsP3) but CCK-8 caused a two times higher accumulation than HG-17 or CCK-39. Tritium 85-87 cholecystokinin Oryctolagus cuniculus 17-20 1714275-5 1991 HG-17, CCK-8 and CCK-39 induced a rapid accumulation of [3H]inositol monophosphate ([3H]InsP1), [3H]inositol bisphosphate ([3H]InsP2) and [3H]inositol trisphosphate ([3H]InsP3) but CCK-8 caused a two times higher accumulation than HG-17 or CCK-39. Tritium 85-87 cholecystokinin Oryctolagus cuniculus 17-20 1714275-5 1991 HG-17, CCK-8 and CCK-39 induced a rapid accumulation of [3H]inositol monophosphate ([3H]InsP1), [3H]inositol bisphosphate ([3H]InsP2) and [3H]inositol trisphosphate ([3H]InsP3) but CCK-8 caused a two times higher accumulation than HG-17 or CCK-39. Tritium 85-87 cholecystokinin Oryctolagus cuniculus 17-20 1714275-5 1991 HG-17, CCK-8 and CCK-39 induced a rapid accumulation of [3H]inositol monophosphate ([3H]InsP1), [3H]inositol bisphosphate ([3H]InsP2) and [3H]inositol trisphosphate ([3H]InsP3) but CCK-8 caused a two times higher accumulation than HG-17 or CCK-39. Tritium 85-87 cholecystokinin Oryctolagus cuniculus 7-10 1714275-5 1991 HG-17, CCK-8 and CCK-39 induced a rapid accumulation of [3H]inositol monophosphate ([3H]InsP1), [3H]inositol bisphosphate ([3H]InsP2) and [3H]inositol trisphosphate ([3H]InsP3) but CCK-8 caused a two times higher accumulation than HG-17 or CCK-39. Tritium 85-87 cholecystokinin Oryctolagus cuniculus 17-20 1714275-5 1991 HG-17, CCK-8 and CCK-39 induced a rapid accumulation of [3H]inositol monophosphate ([3H]InsP1), [3H]inositol bisphosphate ([3H]InsP2) and [3H]inositol trisphosphate ([3H]InsP3) but CCK-8 caused a two times higher accumulation than HG-17 or CCK-39. Tritium 85-87 cholecystokinin Oryctolagus cuniculus 17-20 1714275-5 1991 HG-17, CCK-8 and CCK-39 induced a rapid accumulation of [3H]inositol monophosphate ([3H]InsP1), [3H]inositol bisphosphate ([3H]InsP2) and [3H]inositol trisphosphate ([3H]InsP3) but CCK-8 caused a two times higher accumulation than HG-17 or CCK-39. Tritium 85-87 cholecystokinin Oryctolagus cuniculus 17-20 1714275-5 1991 HG-17, CCK-8 and CCK-39 induced a rapid accumulation of [3H]inositol monophosphate ([3H]InsP1), [3H]inositol bisphosphate ([3H]InsP2) and [3H]inositol trisphosphate ([3H]InsP3) but CCK-8 caused a two times higher accumulation than HG-17 or CCK-39. Tritium 85-87 cholecystokinin Oryctolagus cuniculus 7-10 1714275-5 1991 HG-17, CCK-8 and CCK-39 induced a rapid accumulation of [3H]inositol monophosphate ([3H]InsP1), [3H]inositol bisphosphate ([3H]InsP2) and [3H]inositol trisphosphate ([3H]InsP3) but CCK-8 caused a two times higher accumulation than HG-17 or CCK-39. Tritium 85-87 cholecystokinin Oryctolagus cuniculus 17-20 1714275-5 1991 HG-17, CCK-8 and CCK-39 induced a rapid accumulation of [3H]inositol monophosphate ([3H]InsP1), [3H]inositol bisphosphate ([3H]InsP2) and [3H]inositol trisphosphate ([3H]InsP3) but CCK-8 caused a two times higher accumulation than HG-17 or CCK-39. Tritium 85-87 cholecystokinin Oryctolagus cuniculus 17-20 2123983-5 1990 The 3H-AFB1-metabolite-binding to the nasal glands in vitro was inhibited by the cytochrome P-450-inhibitor metyrapone and by CO- and N2-atmospheres indicating a cytochrome P-450-dependent bioactivation of the AFB1 in these glands. Tritium 4-6 cytochrome P450, family 21, subfamily a, polypeptide 1 Mus musculus 162-178 2115518-5 1990 In contrast, if the differentiated HL-60 cells were supplemented with 10 microM arachidonic acid for 24 h and then stimulated with the ionophore, there was a large amount of [3H]PAF formed. Tritium 175-177 PCNA clamp associated factor Homo sapiens 178-181 2383597-9 1990 EGF receptor synthesis was also measured by immunoprecipitation of [3H]leucine-labeled EGF receptor protein with anti-hEGF receptor monoclonal antibody. Tritium 68-70 epidermal growth factor Homo sapiens 0-3 2383597-9 1990 EGF receptor synthesis was also measured by immunoprecipitation of [3H]leucine-labeled EGF receptor protein with anti-hEGF receptor monoclonal antibody. Tritium 68-70 epidermal growth factor Homo sapiens 87-90 2166488-2 1990 The growth of adherent synovial cells from six of nine samples, determined by [3H]thymidine incorporation, was inhibited by rIFN gamma in a manner dependent on dose. Tritium 79-81 interferon gamma Rattus norvegicus 124-134 2166675-3 1990 In the frontal cortex, the mu agonist, [D-Ala2,MePhe4,Gly-ol5]enkephalin (DAGO, 0.01-1 microM), also inhibited the evoked release of tritium. Tritium 133-140 proenkephalin Rattus norvegicus 62-72 1714275-5 1991 HG-17, CCK-8 and CCK-39 induced a rapid accumulation of [3H]inositol monophosphate ([3H]InsP1), [3H]inositol bisphosphate ([3H]InsP2) and [3H]inositol trisphosphate ([3H]InsP3) but CCK-8 caused a two times higher accumulation than HG-17 or CCK-39. Tritium 85-87 cholecystokinin Oryctolagus cuniculus 17-20 3943078-1 1986 Male Swiss mice and Wistar rats were treated topically with 250 nmol/mouse and 750 nmol/rat of [3H]benzo[a]pyrene ([3H]BaP). Tritium 96-98 prohibitin 2 Rattus norvegicus 119-122 1859357-4 1991 [3H]Glucosamine-labelled HSPG from human skin fibroblasts was depolymerized by using HNO2 or heparinase under conditions that allowed cleavage of all susceptible linkages. Tritium 1-3 glypican 3 Homo sapiens 25-29 2384133-0 1990 The neuropeptides vasoactive intestinal polypeptide, peptide histidine isoleucine and neuropeptide Y modulate [3H]noradrenaline release from sympathetic nerves in the choroid plexus. Tritium 111-113 vasoactive intestinal peptide Sus scrofa 18-51 2159529-2 1990 [3H]glucosamine labeling of cells infected with vaccinia virus recombinants that expressed the BILF2 open reading frame revealed several diffuse species of glycoproteins of around 80,000 and 55,000 daltons. Tritium 1-3 membrane protein BILF2 Human gammaherpesvirus 4 95-100 3036937-13 1986 IgM and IgG2a containing conjugates caused suppression of [3H]thymidine incorporation into PLC/PRF/5 cells. Tritium 59-61 heparan sulfate proteoglycan 2 Homo sapiens 91-94 2114000-9 1990 E2 potentiated (p less than 0.01) the effects of veratridine, C8, PLC, and GnRH on medium IRLH, and medium and total [3H]glucosamine-LH. Tritium 118-120 gonadotropin releasing hormone 1 Rattus norvegicus 75-79 1859467-11 1991 [3H]Thymidine incorporation was further increased significantly (P less than 0.05) 24 hr after challenge with a low dose of CCl4 in PH5 gerbils, whereas it remained low until 48 hr after the CCl4 injection in the PH15 or SH group. Tritium 1-3 C-C motif chemokine ligand 4 Homo sapiens 124-128 1859467-11 1991 [3H]Thymidine incorporation was further increased significantly (P less than 0.05) 24 hr after challenge with a low dose of CCl4 in PH5 gerbils, whereas it remained low until 48 hr after the CCl4 injection in the PH15 or SH group. Tritium 1-3 C-C motif chemokine ligand 4 Homo sapiens 191-195 1647310-3 1991 Des-IGF I and IGF I increased [3H] thymidine incorporation into DNA, [3H]proline incorporation into collagen and noncollagen protein, and the mitotic index in intact calvariae. Tritium 31-33 insulin-like growth factor 1 Rattus norvegicus 4-9 1647310-3 1991 Des-IGF I and IGF I increased [3H] thymidine incorporation into DNA, [3H]proline incorporation into collagen and noncollagen protein, and the mitotic index in intact calvariae. Tritium 31-33 insulin-like growth factor 1 Rattus norvegicus 14-19 1647310-3 1991 Des-IGF I and IGF I increased [3H] thymidine incorporation into DNA, [3H]proline incorporation into collagen and noncollagen protein, and the mitotic index in intact calvariae. Tritium 70-72 insulin-like growth factor 1 Rattus norvegicus 4-9 1647310-3 1991 Des-IGF I and IGF I increased [3H] thymidine incorporation into DNA, [3H]proline incorporation into collagen and noncollagen protein, and the mitotic index in intact calvariae. Tritium 70-72 insulin-like growth factor 1 Rattus norvegicus 14-19 2344437-2 1990 On the other hand, 3H-labeled diacylglycerol co-emulsified with non-radioactive phosphatidylcholine was found to be a poor substrate for the diacylglycerol lipase. Tritium 19-21 lipase G, endothelial type Rattus norvegicus 156-162 1695530-10 1990 The selective NK1-receptor agonist produced a dose-dependent increase in cell proliferation as judged by total cell number and [3H]-thymidine uptake. Tritium 128-130 tachykinin receptor 1 Homo sapiens 14-26 2419773-8 1986 Any hindrance of deamination (by tritium in position 8, COMT intact) leads to a shift of the metabolism of the 3H-catecholamines from the exclusively deaminated to the exclusively O-methylated metabolites. Tritium 33-40 catechol-O-methyltransferase Rattus norvegicus 56-60 2328692-7 1990 Data are also presented which indicate that [3H]E2 exogenously added to the extracellular medium is in simple equilibrium with the intracellular compartment and the ER. Tritium 45-47 estrogen receptor 1 Rattus norvegicus 165-167 1664945-4 1991 In contrast, SHR had lower [3H]DAGO binding sites in the CA1 of the hippocampus. Tritium 28-30 carbonic anhydrase 1 Rattus norvegicus 57-60 2936365-6 1985 In in vitro radioligand binding studies, RDS-127 potently displaced [3H]DPAT binding to 5-HT1A sites in rat cortex (Ki = 14 +/- 4 nM) and was only moderately effective in displacing [3H]spiperone binding to dopaminergic D2 sites in rat striatum. Tritium 69-71 peripherin 2 Rattus norvegicus 41-44 1904062-4 1991 TPA inhibited [3H]thymidine incorporation in GH4 cells in a stereoselective and concentration-dependent manner. Tritium 15-17 plasminogen activator, tissue type Rattus norvegicus 0-3 2159287-5 1990 The concentration-dependence of EGF-stimulated 1,2-diacylglycerol production and [3H]thymidine incorporation were similar. Tritium 82-84 epidermal growth factor Homo sapiens 32-35 3905974-6 1985 Both [32P] phosphate and [3H]histidine were incorporated into the high-Mr band after pulse labeling for 3 h. After a 15-h chase, [3H]histidine, but not [32P]phosphate appeared in filaggrin. Tritium 130-132 filaggrin Homo sapiens 179-188 2318161-3 1990 To assess the binding characteristics of [3H]estradiol-receptor complexes (3HER) to nuclear matrix, ER from ovariectomized mice was partially purified by ammonium sulfate precipitation and incubated under cell-free conditions with mouse uterine nuclear matrix at 4 C. The binding capacity of the nuclear matrix was determined to be 36.4 +/- 5.7 fmol/100 micrograms DNA, with a Kd of 0.23 +/- 0.03 nM. Tritium 42-44 estrogen receptor 1 (alpha) Mus musculus 77-79 3904924-6 1985 It also appeared necessary to have inhibitors of both monoamine oxidase (pargyline) and catecholamine-O-methyltransferase (tropolone) present to see clear cellular localization for [3H]DA. Tritium 182-184 catechol-O-methyltransferase Rattus norvegicus 54-121 1652797-5 1991 administration of beta-FNA alters the conformation of the opioid receptor complex, as inferred by a decrease in the Bmax of the lower affinity [3H][D-ala2,D-leu5]enkephalin binding site. Tritium 144-146 proenkephalin Rattus norvegicus 162-172 4079371-5 1985 Analyses of these partially purified estrogen receptor fractions using high-salt sucrose density gradients showed that Peak I [3H]estradiol-receptor complexes sedimented predominantly as a lighter form (4.0S). Tritium 127-129 estrogen receptor 1 Rattus norvegicus 37-54 1652797-6 1991 Consistent with the decreased potency of naloxone as an inhibitor of delta receptor mediated effects, beta-FNA doubled the naloxone IC50 for displacing [3H][D-ala2,D-leu5]enkephalin from its lower affinity binding site. Tritium 153-155 proenkephalin Rattus norvegicus 171-181 2370005-1 1990 The chromosomal localization of the mouse gene coding for desmin, one of the muscle-specific intermediate filament subunits, was determined by in situ hybridization using a specific 3H-labelled DNA probe. Tritium 182-184 desmin Mus musculus 58-64 1648928-6 1991 NMDA-sensitive [3H]-glutamate binding site density was reduced significantly by 19 to 29% in the apical dendritic field regions of dentate gyrus, hippocampal CA1 and subiculum of dorsal hippocampal formation of fetal alcohol rats compared to pair-fed and ad lib controls. Tritium 16-18 carbonic anhydrase 1 Rattus norvegicus 158-161 4084522-2 1985 Protein 3, the major protein of the erythrocyte membrane, functions in anion transport and reacts covalently with tritiated 4,4"-diisothiocyano-1,2-diphenylethane-2,2"-disulfonic acid ([3H]DIDS), a very selective inhibitor of anion transport. Tritium 186-188 heat shock protein family B (small) member 3 Homo sapiens 0-9 2109157-2 1990 The human rIL2 secreted by this high-producing recombinant CHO cell line was metabolically radiolabelled with [35S]-methionine, or with [3H]-glucosamine and [3H]-galactose, purified to homogeneity, and then characterized. Tritium 137-139 interleukin 2 Rattus norvegicus 10-14 2109157-2 1990 The human rIL2 secreted by this high-producing recombinant CHO cell line was metabolically radiolabelled with [35S]-methionine, or with [3H]-glucosamine and [3H]-galactose, purified to homogeneity, and then characterized. Tritium 158-160 interleukin 2 Rattus norvegicus 10-14 2109157-5 1990 The point of attachment and the structure of the carbohydrate moiety of the rIL2 was determined by: amino-terminal sequencing and fingerprint analysis of the 3H-labelled rIL2, mass spectroscopy of the amino-terminal tryptic octapeptide, and carbohydrate analysis after enzymatic (Vibrio cholerae neuraminidase and Aspergillus oryzae beta-galactosidase) or sulfuric acid hydrolysis. Tritium 158-160 interleukin 2 Rattus norvegicus 76-80 2109157-5 1990 The point of attachment and the structure of the carbohydrate moiety of the rIL2 was determined by: amino-terminal sequencing and fingerprint analysis of the 3H-labelled rIL2, mass spectroscopy of the amino-terminal tryptic octapeptide, and carbohydrate analysis after enzymatic (Vibrio cholerae neuraminidase and Aspergillus oryzae beta-galactosidase) or sulfuric acid hydrolysis. Tritium 158-160 interleukin 2 Rattus norvegicus 170-174 4084522-3 1985 In this study, [3H]DIDS was used to label protein 3 in the membranes of normal cells and those from a donor heterozygous for a variant of protein 3, defined by its elongated amino-terminal end. Tritium 16-18 heat shock protein family B (small) member 3 Homo sapiens 42-51 1709867-7 1991 TcR gamma/delta+ cells obtained from a patient with acute lymphoblastic leukemia 3 months after autologous bone marrow transplantation were induced to proliferate, as measured by [3H]thymidine incorporation after stimulation with anti-CD7 mAb but not with anti-CD3 mAb. Tritium 180-182 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 0-3 1708810-9 1991 Both Rh123 fractions proliferated in response to MGF alone as determined by a [3H]TdR assay or by counting nucleated cells present in the cultures over time. Tritium 79-81 kit ligand Mus musculus 49-52 1652776-9 1991 Incubation of progenitor cells with IGF-I resulted in a time- and concentration-dependent increase in [3H]thymidine incorporation and cell numbers. Tritium 103-105 insulin-like growth factor 1 Rattus norvegicus 36-41 2156494-5 1990 [3H]Orotate incorporation as uridine into kidney poly(A)+ RNA was stimulated by aminophylline, a potent inducer of 25-hydroxyvitamin D3 24-hydroxylase; however, the amount of nascent ferredoxin synthesis was the same as in normal controls. Tritium 1-3 cytochrome P450 family 24 subfamily A member 1 Gallus gallus 115-150 4084522-3 1985 In this study, [3H]DIDS was used to label protein 3 in the membranes of normal cells and those from a donor heterozygous for a variant of protein 3, defined by its elongated amino-terminal end. Tritium 16-18 heat shock protein family B (small) member 3 Homo sapiens 138-147 2016326-3 1991 Treatment of exponentially growing MRC5 cells with these cytokines led to a 3-4-fold increase in transferrin receptor mRNA and a coordinate increase in transferrin receptor protein by 24 h. Under these conditions, stimulation of [3H]thymidine incorporation was minimal, suggesting that the induction of transferrin receptor by TNF and IL-1 is mediated by a growth-independent regulatory mechanism. Tritium 230-232 interleukin 1 alpha Homo sapiens 335-339 3001971-2 1985 The final preparation migrated as a single protein band corresponding to a radioactive peak of [3H]PAF bound on polyacrylamide disc gel electrophoresis. Tritium 96-98 PCNA clamp associated factor Homo sapiens 99-102 1713165-1 1991 An overflow of 3H evoked by 3,4-DAP (representing [3H]NA release) was measurable not only in the presence but also in the absence of extracellular Ca2+. Tritium 15-17 death-associated protein Rattus norvegicus 32-35 1713165-1 1991 An overflow of 3H evoked by 3,4-DAP (representing [3H]NA release) was measurable not only in the presence but also in the absence of extracellular Ca2+. Tritium 51-53 death-associated protein Rattus norvegicus 32-35 1713165-3 1991 Moreover, in the absence of extracellular Ca2+, both the 3,4-DAP-evoked [3H]NA release and the facilitatory effect of 4 beta-PDB were abolished in the presence of tetrodotoxin or in the absence of Na+ in the superfusion medium. Tritium 73-75 death-associated protein Rattus norvegicus 61-64 1713165-4 1991 Ruthenium red, a blocker of mitochondrial Ca2+ reuptake, potently increased 3,4-DAP-evoked [3H]NA release in Ca(2+)-free EGTA-containing medium. Tritium 92-94 death-associated protein Rattus norvegicus 80-83 3001971-4 1985 Exposure of the platelet membranes to an excess of unlabeled PAF diminished the binding of [3H]PAF. Tritium 92-94 PCNA clamp associated factor Homo sapiens 61-64 1713165-6 1991 From these and earlier observations we conclude (1) that the mechanism of 3,4-DAP-evoked [3H]NA release involves both Ca2+ influx into the nerve terminals and mobilization of intraneuronal Ca2+ pools. Tritium 90-92 death-associated protein Rattus norvegicus 78-81 3001971-4 1985 Exposure of the platelet membranes to an excess of unlabeled PAF diminished the binding of [3H]PAF. Tritium 92-94 PCNA clamp associated factor Homo sapiens 95-98 4041865-7 1985 These findings suggest that 5-HT1A, and 5-HT1B binding sites have distinct pharmacological profiles and can be directly labeled with appropriate [3H]ligands in specific brain regions. Tritium 146-148 5-hydroxytryptamine receptor 1A Bos taurus 28-34 1847855-9 1991 Isoproterenol enhanced the ability of IGF-I to stimulate [3H]thymidine incorporation in BAR but not RBAR cells. Tritium 58-60 insulin-like growth factor 1 Rattus norvegicus 38-43 4044568-2 1985 The EF-Ts-catalyzed exchange rates were measured at a series of concentrations of EF-Tu X [3H] GDP and free nucleotide. Tritium 91-93 Ts translation elongation factor, mitochondrial Homo sapiens 4-9 2005325-6 1991 There was a good correlation (r = 0.95) between PAF quantified by [3H]acetic acid incorporation and by rabbit platelet aggregation bioassay, indicating that the radioactive material is PAF. Tritium 67-69 PCNA clamp associated factor Homo sapiens 48-51 2005325-6 1991 There was a good correlation (r = 0.95) between PAF quantified by [3H]acetic acid incorporation and by rabbit platelet aggregation bioassay, indicating that the radioactive material is PAF. Tritium 67-69 PCNA clamp associated factor Homo sapiens 185-188 3898866-9 1985 Sparse, serum-starved smooth muscle cells responded to insulin with a dose-dependent increase in [3H]-thymidine incorporation into DNA. Tritium 98-100 insulin Bos taurus 55-62 1906085-5 1991 The net mass secretion and synthesis ([3H]leucine incorporation) of apoA-I by perfused livers from male hyperthyroid rats was elevated, while secretion of albumin was not different than that of euthyroid rats. Tritium 39-41 apolipoprotein A1 Rattus norvegicus 68-74 1672374-5 1991 The neuroleptics thioridazine and chlorpromazine also decreased thrombin-stimulated [3H]IP2 and [3H]IP3 production but not [3H]IP1 in human platelets, whereas haloperidol had no significant effect. Tritium 85-87 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 88-91 4042784-4 1985 Moreover, significant differences in G2 nuclear protein levels were found between untreated and 3H-thymidine-treated lymphocytes. Tritium 96-98 proline rich coiled-coil 2A Homo sapiens 37-39 4078571-2 1985 The orientation of tritium at the diastereotopic C-2 methylene positions of cephalosporin C has been determined in a kinetic assay involving its conversion to N-t-butoxycarbonyldeacetylcephalosporin C-1beta-oxide and monitoring the loss of tritium at constant pH, ionic strength, and temperature. Tritium 19-26 complement C2 Homo sapiens 49-52 2001419-2 1991 12-O-Tetradecanoyl phorbol 13-acetate (TPA) doubled ionomycin-induced PLA2 activity, assessed by [3H]arachidonate release. Tritium 98-100 phospholipase A2 group IB Rattus norvegicus 70-74 4078571-2 1985 The orientation of tritium at the diastereotopic C-2 methylene positions of cephalosporin C has been determined in a kinetic assay involving its conversion to N-t-butoxycarbonyldeacetylcephalosporin C-1beta-oxide and monitoring the loss of tritium at constant pH, ionic strength, and temperature. Tritium 240-247 complement C2 Homo sapiens 49-52 1705511-2 1991 CD5+ cells incorporated [3H]thymidine more efficiently than unfractionated cells when stimulated with SAC and their response was augmented by the addition of interleukin (IL) 2 to the cultures. Tritium 25-27 CD5 molecule Homo sapiens 0-3 4078571-4 1985 An equal distribution to tritium between the two C-2 loci was observed accompanied by a large intrinsic isotope effect. Tritium 25-32 complement C2 Homo sapiens 49-52 4015102-0 1985 Hydrogen-tritium exchange by apoferritin and ferritin. Tritium 9-16 ferritin heavy chain 1 Homo sapiens 29-40 1675834-1 1991 The binding of the quaternary radioligand [3H] QMDP to the histamine H1-receptor was inhibited by a series of mono- and di-valent cations. Tritium 43-45 histamine receptor H1 Homo sapiens 59-80 1835598-3 1991 Dihydrofolate reductase (DHFR) activity was increased 63-fold in 11B-MTX-C cells together with a decrease in [3H]-methotrexate transport and folylpolyglutamate synthase (FPGS) activity (2.5-fold and 3.8-fold, respectively). Tritium 110-112 dihydrofolate reductase Homo sapiens 0-23 1835598-3 1991 Dihydrofolate reductase (DHFR) activity was increased 63-fold in 11B-MTX-C cells together with a decrease in [3H]-methotrexate transport and folylpolyglutamate synthase (FPGS) activity (2.5-fold and 3.8-fold, respectively). Tritium 110-112 dihydrofolate reductase Homo sapiens 25-29 1766257-5 1991 With IL-2, 3H-TdR incorporation increased in cells from 5 out of 10 T-ALL patients, including those with CD7+4-8-, CD5+, and CD1+ acute leukemia. Tritium 11-13 CD5 molecule Homo sapiens 115-118 1766257-6 1991 In the presence of IL-3 or IL-6, 3H-TdR incorporation increased in cells from 2 out of 5 patients with CD7+4-8- acute leukemia. Tritium 33-35 interleukin 3 Homo sapiens 19-23 1992286-0 1991 Irreversible blockade of the high and low affinity (3H) naloxone binding sites by C-6 derivatives of morphinane-6-ones. Tritium 51-55 complement C6 Rattus norvegicus 82-85 4015102-1 1985 The out-exchange kinetics of tritium from apoferritin, ferritin of various iron contents, and apoferritin subunits were examined. Tritium 29-36 ferritin heavy chain 1 Homo sapiens 42-53 2988760-8 1985 A simple, rapid assay for a new human CSA with pluripotent hematopoietic stimulating activity (pluripoietin) is described based on stimulation of [3H]glucosamine incorporation. Tritium 147-149 colony stimulating factor 3 Homo sapiens 95-107 1846021-4 1991 After 23 h of treatment, 60 to 6,000 pM activin-A increased the rate of [3H]thymidine incorporation into DNA 1.5- to 4.0-fold, and at 600 to 6,000 pM, it enhanced the rate of [3H]proline incorporation into collagen and noncollagen protein by up to 1.7-fold. Tritium 73-75 inhibin subunit beta A Rattus norvegicus 40-49 1846021-4 1991 After 23 h of treatment, 60 to 6,000 pM activin-A increased the rate of [3H]thymidine incorporation into DNA 1.5- to 4.0-fold, and at 600 to 6,000 pM, it enhanced the rate of [3H]proline incorporation into collagen and noncollagen protein by up to 1.7-fold. Tritium 176-178 inhibin subunit beta A Rattus norvegicus 40-49 2989719-1 1985 [Tyr-3,5-3H]1, D-Ala2, Leu5-enkephalin [( 3H]DALA) was used for labeling the opioid receptors of rat brain plasma membranes. Tritium 9-11 proenkephalin Rattus norvegicus 28-38 1814006-2 1991 PA-I, the galactophilic lectin, exhibited both cytotoxic and cytostatic activities on these cells (tested by [3H]thymidine incorporation and by crystal violet vital staining). Tritium 110-112 serine (or cysteine) peptidase inhibitor, clade E, member 1 Mus musculus 0-4 2986762-2 1985 ADX for one week causes an increase in [3H]5-HT binding to 5-HT1 receptors which is significant in the CA1 cell field. Tritium 40-42 carbonic anhydrase 1 Rattus norvegicus 103-106 2125219-2 1990 Addition of growth hormone to Ob1771 cells prelabelled with [3H]glycerol or [3H]choline led to a rapid, transient and stoechiometric formation of labelled diacylglycerol and phosphocholine, respectively. Tritium 61-63 growth hormone Mus musculus 12-26 2125219-3 1990 In contrast, no change was observed in the level of choline and phosphatidic acid whereas the release of water-soluble metabolites in [3H]ethanolamine prelabelled cells exposed to growth hormone was hardly detectable. Tritium 135-137 growth hormone Mus musculus 180-194 3921023-1 1985 Activity of thymidylate synthase was measured in situ in leukemia cells by tritium release from [5-3H]dUrd. Tritium 75-82 thymidylate synthetase Homo sapiens 12-32 2983026-6 1985 AVM at submicromolar concentrations had the opposite effect of pentobarbital and etazolate on GABA receptor binding, decreasing by half the high-affinity binding of [3H]GABA and related agonist ligands, and increasing by over twofold the binding of the antagonist [3H]bicuculline methochloride, an effect that was potentiated by picrotoxin. Tritium 166-168 GABA type A receptor-associated protein Homo sapiens 94-107 2266115-9 1990 Cells, PKC-depleted by chronical phorbol ester treatment, responded to IL-3 or IL-4 with a significant increase in [3H] thymidine uptake over PKC containing cells stimulated with the same lymphokine. Tritium 116-118 interleukin 4 Mus musculus 79-83 3156935-1 1985 Interleukin 2 (IL-2) activity is tested in conditioned media by assessing its ability to support proliferation of selected IL-2 dependent T cell lines, conventionally measured by [3H]thymidine incorporation. Tritium 180-182 interleukin 2 Mus musculus 0-13 2246516-6 1990 When exogenous [3H]PAF was added to the two stimulated eosinophil populations subsequent analysis of the [3H]PAF metabolites by DIOL-HPLC revealed that low density eosinophils incorporated PAF into the phosphatidylcholine (PC) pool more rapidly than did normal density eosinophils or neutrophils. Tritium 16-18 PCNA clamp associated factor Homo sapiens 19-22 2246516-6 1990 When exogenous [3H]PAF was added to the two stimulated eosinophil populations subsequent analysis of the [3H]PAF metabolites by DIOL-HPLC revealed that low density eosinophils incorporated PAF into the phosphatidylcholine (PC) pool more rapidly than did normal density eosinophils or neutrophils. Tritium 106-108 PCNA clamp associated factor Homo sapiens 19-22 3156935-1 1985 Interleukin 2 (IL-2) activity is tested in conditioned media by assessing its ability to support proliferation of selected IL-2 dependent T cell lines, conventionally measured by [3H]thymidine incorporation. Tritium 180-182 interleukin 2 Mus musculus 15-19 3156935-2 1985 Here, we compare this [3H]thymidine uptake test for measuring IL-2 activity with a rapid and sensitive colorimetric method which is based on the ability of viable cells to cleave 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). Tritium 23-25 interleukin 2 Mus musculus 62-66 1701215-4 1990 TPO and Bgt also share the capacity for high affinity interaction with muscle-type nAcChoR, which are expressed as high affinity binding sites for radioiodinated Bgt or tritium-labeled acetylcholine by cells of the TE671/RD human clone or the BC3H-1 mouse muscle cell line or on membrane preparations from Torpedo electroplax. Tritium 169-176 thymopoietin Homo sapiens 0-3 2984256-6 1985 The ability to inhibit release of [3H]serotonin from washed human platelets was at least 10 times less using human thrombomodulin compared with rabbit thrombomodulin. Tritium 35-37 thrombomodulin Homo sapiens 115-129 2579319-0 1985 A quantitative study of [3H]D-Ala2-D-Leu5-enkephalin binding to rat brain membranes. Tritium 25-27 proenkephalin Rattus norvegicus 42-52 2208144-2 1990 Both the ASC and STC cell preparations contain elevated levels of the sialomucin ASGP-1 relative to TUN and LTC preparations as determined by [3H]glucosamine labeling and by binding of peanut agglutinin. Tritium 143-145 endomucin Rattus norvegicus 70-80 2579319-4 1985 In this study, we examine the interaction of the mu agonist oxymorphone and delta agonist DSTLE (D-Ser2-Thr6-Leu-enkephalin) with [3H]D-Ala2-D-Leu5-enkephalin (DADL) binding to membranes of rat brain. Tritium 131-133 proenkephalin Rattus norvegicus 113-123 1699770-4 1990 The NPB assembly occurred only in pronuclei which incorporated [3H]-adenosine. Tritium 63-68 neuropeptide B Homo sapiens 4-7 2579319-4 1985 In this study, we examine the interaction of the mu agonist oxymorphone and delta agonist DSTLE (D-Ser2-Thr6-Leu-enkephalin) with [3H]D-Ala2-D-Leu5-enkephalin (DADL) binding to membranes of rat brain. Tritium 131-133 proenkephalin Rattus norvegicus 148-158 3918224-4 1985 On the other hand, neither [3H]ligand binding was sensitive to phospholipases C and D. In contrast, prior treatment with phospholipase A2 (unheated) drastically decreased the [3H]5-HT binding and also affected the [3H]spiperone binding to some extent. Tritium 176-178 phospholipase A2 group IB Rattus norvegicus 121-137 1700082-3 1990 A tritium-labeled aminoterminal heptapeptide of SP, 3H-SP(1-7), was synthesized, purified, and used to characterize the binding of a variety of fragments of SP and opioids in the mouse brain and spinal cord membranes. Tritium 2-9 tachykinin 1 Mus musculus 48-50 1700082-3 1990 A tritium-labeled aminoterminal heptapeptide of SP, 3H-SP(1-7), was synthesized, purified, and used to characterize the binding of a variety of fragments of SP and opioids in the mouse brain and spinal cord membranes. Tritium 2-9 tachykinin 1 Mus musculus 55-57 1700082-3 1990 A tritium-labeled aminoterminal heptapeptide of SP, 3H-SP(1-7), was synthesized, purified, and used to characterize the binding of a variety of fragments of SP and opioids in the mouse brain and spinal cord membranes. Tritium 2-9 tachykinin 1 Mus musculus 55-57 2177450-3 1990 C3d, fixed on zymosan or attached to the culture wells, induced [3H]thymidine incorporation of the B cells in serum-free medium. Tritium 65-67 endogenous retrovirus group K member 13 Homo sapiens 0-3 3918224-4 1985 On the other hand, neither [3H]ligand binding was sensitive to phospholipases C and D. In contrast, prior treatment with phospholipase A2 (unheated) drastically decreased the [3H]5-HT binding and also affected the [3H]spiperone binding to some extent. Tritium 176-178 phospholipase A2 group IB Rattus norvegicus 121-137 3918224-7 1985 In addition, the reduction of [3H]5-HT binding capacity in membranes treated with phospholipase A2 (heated and unheated) was restored only slightly by treatment with BSA (1%). Tritium 31-33 phospholipase A2 group IB Rattus norvegicus 82-98 1703022-5 1990 At the same concentration, rabbit serum albumin (RSA) also significantly protected [3H]PAF C18:0 from metabolism, but bovine gamma globulin (BGG) was ineffective. Tritium 84-86 albumin Bos taurus 34-47 3918224-8 1985 Scatchard analysis of the [3H]5-HT binding showed that Azure A and phospholipase A2 (heated) decreased the Bmax values with no significant alteration in the KD values, whereas arylsulphatase increased only the KD value. Tritium 27-29 phospholipase A2 group IB Rattus norvegicus 67-83 2986652-1 1985 The influence of pH on binding of labeled stable analog of enkephalin, [3H]Tyr1, D-Ala2, D-Leu5]enkephalin, to high- and low-affinity receptors of rat brain membranes was studied. Tritium 72-74 proenkephalin Rattus norvegicus 59-69 2986652-1 1985 The influence of pH on binding of labeled stable analog of enkephalin, [3H]Tyr1, D-Ala2, D-Leu5]enkephalin, to high- and low-affinity receptors of rat brain membranes was studied. Tritium 72-74 proenkephalin Rattus norvegicus 96-106 2177154-5 1990 Kappa 1 binding sites measured in the presence of 5 microM/D-Ala2-Leu5/enkephalin represent 25-30% of [3H]ethylketocyclazocine binding in frog brain membranes. Tritium 103-105 proenkephalin Rattus norvegicus 71-81 2981664-8 1985 In time course experiments, increased [3H]1,25-(OH)2D3 accumulation could be observed before the detection of 24-hydroxylase activity suggesting that the primary effect of PTH and forskolin is on the production of [3H] 1,25-(OH)2D3 rather than its catabolism. Tritium 39-41 parathyroid hormone Gallus gallus 172-175 2201895-8 1990 The released TGF alpha was bioactive, as it was capable of both competing with epidermal growth factor for binding to its receptor and stimulating [3H]thymidine incorporation in the mitogenic assay. Tritium 148-150 transforming growth factor alpha Mus musculus 13-22 2981664-8 1985 In time course experiments, increased [3H]1,25-(OH)2D3 accumulation could be observed before the detection of 24-hydroxylase activity suggesting that the primary effect of PTH and forskolin is on the production of [3H] 1,25-(OH)2D3 rather than its catabolism. Tritium 215-217 parathyroid hormone Gallus gallus 172-175 3965489-8 1985 Androsterone formation from [3H]3 alpha-diol, however, was linear with time of incubation for 4 h (8.9 pmol 100 mg-1 tissue 4 h-1). Tritium 29-31 H1.5 linker histone, cluster member Homo sapiens 126-129 1699196-3 1990 The fyn protein from IM-9 cells incorporated [3H]myristate in vivo and was found to be membrane associated. Tritium 46-48 FYN proto-oncogene, Src family tyrosine kinase Homo sapiens 4-7 3871462-2 1985 The assay is based on the fact that culture supernatants from contaminated cells inhibit [3H]thymidine incorporation by an IL-2 dependent mouse cytotoxic T cell line (CTLL). Tritium 90-92 interleukin 2 Mus musculus 123-127 2390085-5 1990 Furthermore, the PPH inhibitor, propranolol, inhibited the formation of both [3H]alkyl-DG and [32P]PO4, while increasing alkyl-PA levels (containing both 3H and 32P). Tritium 78-80 enolase 1 Homo sapiens 17-20 2990161-3 1985 We have identified alpha 1- and alpha 2-adrenergic receptors in myometrial membranes using the newly developed radiolabelled specific antagonists [3H]-prazosin and [3H]-rauwolscine. Tritium 147-150 adrenoceptor alpha 1D Homo sapiens 19-26 2198284-13 1990 Selective periodate oxidation showed that both the inner and outer sialic acid residues of GD3 incorporated 3H-label in the in vitro reaction, and showed similar turnover of N-acetylation in the pulse-chase study. Tritium 108-110 GRDX Homo sapiens 91-94 3009068-3 1985 Stimulation of the pituitary tumor by synthetic ovine corticotropin-releasing factor (CRF 1-41) caused a 171-468% increase in vivo (60 min) and 453-953% increase in vitro (3h incubation) in the levels of POMC derived peptides; it increased the relative amount of beta-END in vivo, and that of beta-LPH in vitro. Tritium 172-174 corticotropin releasing hormone Homo sapiens 54-84 2201833-4 1990 The effect of 18-hr culture in purified recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF) (1 ng/ml) on in vitro AML marrow cell [3H]dThd incorporation into DNA, intracellular ara-C activation to the triphosphate form (ara-CTP), and subsequent ara-CTP retention were determined in leukemic cells of 11 patients and compared with cells similarly cultured in HSA-containing sera. Tritium 157-159 colony stimulating factor 2 Homo sapiens 58-106 2171047-7 1990 This observation led us to perform a competitive binding assay between DBI and [3H]PK11195 (a ligand for the mitochondrial BZ binding sites) on mitochondrial membranes of adrenal cortical cells. Tritium 80-82 diazepam binding inhibitor Rattus norvegicus 71-74 3001111-3 1985 Using a known protein (3H-labeled translation initiation factor [eIF-4D]) as a standard internal marker for isoelectric point (pI), we show that p21 proteins from various cells differ only slightly in molecular weight (21-24 kDa) but express a wide variety in charge (pI 4.8 to 7) that could only be detected by the use of two-dimensional gel electrophoresis. Tritium 23-25 H3 histone pseudogene 16 Homo sapiens 145-148 2197592-1 1990 Using the insect/baculovirus expression system, we demonstrate the incorporation of [3H]mevalonate and [3H]methyl groups into recombinant c-Ha-ras protein (p21). Tritium 85-87 H3 histone pseudogene 16 Homo sapiens 156-159 3871877-8 1985 The binding of [3H]estradiol to the estrogen receptor was highly specific since a strong displacement was only observed with estradiol (83%). Tritium 16-18 estrogen receptor 1 Canis lupus familiaris 36-53 2377281-6 1990 A small series of adrenergic agents competed for the [3H]dihydroalprenolol, [3H]yohimbine, and [3H]praz binding sites in a fashion suggesting the presence of alpha 1-, alpha 2-, beta 1-, and beta 2-receptors on the vessels within human pia. Tritium 53-56 adrenoceptor alpha 1D Homo sapiens 158-197 2377281-6 1990 A small series of adrenergic agents competed for the [3H]dihydroalprenolol, [3H]yohimbine, and [3H]praz binding sites in a fashion suggesting the presence of alpha 1-, alpha 2-, beta 1-, and beta 2-receptors on the vessels within human pia. Tritium 54-56 adrenoceptor alpha 1D Homo sapiens 158-197 2864680-1 1985 The somatostatin analogs D-Phe-Cys-D-Trp-Lys-Thr-Cys-Thr and the corresponding penicillamine compounds have been prepared and tested for their ability to displace [3H]naloxone and [3H] [D-Ala2, D-Leu5]enkephalin from rat brain receptors. Tritium 164-166 proenkephalin Rattus norvegicus 201-211 6438103-3 1984 Burkitt lymphoma cell lines (Ramos, Daudi, Put) were characterized by a high chemical quantity of Gb3, high enzyme activity for synthesis of Gb3 (UDP-Gal:LacCer alpha-galactosyltransferase), and a high degree of surface exposure of Gb3, as determined by galactose oxidase/NaB[3H]4 and by cytofluorometry with the monoclonal antibody to Gb3 (38.13). Tritium 276-278 alpha 1,4-galactosyltransferase (P blood group) Homo sapiens 141-144 1700400-6 1990 The magnitude of SP-induced desensitization was also found to be negatively correlated (r = .95) with total recovered intact [3H]SP. Tritium 126-128 tachykinin 1 Mus musculus 17-19 1700400-6 1990 The magnitude of SP-induced desensitization was also found to be negatively correlated (r = .95) with total recovered intact [3H]SP. Tritium 126-128 tachykinin 1 Mus musculus 129-131 2402522-4 1990 A new simple technique for separating protein bound [3H]PGF2 alpha (PGF2 alpha receptor-[3H]PGF2 alpha complexes) from free [3H]PGF2 alpha by use of hydroxyapatite (HAP) is introduced. Tritium 53-55 prostaglandin F synthase 2 Bos taurus 56-60 2402522-4 1990 A new simple technique for separating protein bound [3H]PGF2 alpha (PGF2 alpha receptor-[3H]PGF2 alpha complexes) from free [3H]PGF2 alpha by use of hydroxyapatite (HAP) is introduced. Tritium 53-55 prostaglandin F synthase 2 Bos taurus 68-72 2402522-4 1990 A new simple technique for separating protein bound [3H]PGF2 alpha (PGF2 alpha receptor-[3H]PGF2 alpha complexes) from free [3H]PGF2 alpha by use of hydroxyapatite (HAP) is introduced. Tritium 53-55 prostaglandin F synthase 2 Bos taurus 68-72 2402522-4 1990 A new simple technique for separating protein bound [3H]PGF2 alpha (PGF2 alpha receptor-[3H]PGF2 alpha complexes) from free [3H]PGF2 alpha by use of hydroxyapatite (HAP) is introduced. Tritium 53-55 prostaglandin F synthase 2 Bos taurus 68-72 2402522-4 1990 A new simple technique for separating protein bound [3H]PGF2 alpha (PGF2 alpha receptor-[3H]PGF2 alpha complexes) from free [3H]PGF2 alpha by use of hydroxyapatite (HAP) is introduced. Tritium 89-91 prostaglandin F synthase 2 Bos taurus 56-60 2402522-4 1990 A new simple technique for separating protein bound [3H]PGF2 alpha (PGF2 alpha receptor-[3H]PGF2 alpha complexes) from free [3H]PGF2 alpha by use of hydroxyapatite (HAP) is introduced. Tritium 89-91 prostaglandin F synthase 2 Bos taurus 68-72 2402522-4 1990 A new simple technique for separating protein bound [3H]PGF2 alpha (PGF2 alpha receptor-[3H]PGF2 alpha complexes) from free [3H]PGF2 alpha by use of hydroxyapatite (HAP) is introduced. Tritium 89-91 prostaglandin F synthase 2 Bos taurus 68-72 2402522-4 1990 A new simple technique for separating protein bound [3H]PGF2 alpha (PGF2 alpha receptor-[3H]PGF2 alpha complexes) from free [3H]PGF2 alpha by use of hydroxyapatite (HAP) is introduced. Tritium 89-91 prostaglandin F synthase 2 Bos taurus 68-72 6438103-3 1984 Burkitt lymphoma cell lines (Ramos, Daudi, Put) were characterized by a high chemical quantity of Gb3, high enzyme activity for synthesis of Gb3 (UDP-Gal:LacCer alpha-galactosyltransferase), and a high degree of surface exposure of Gb3, as determined by galactose oxidase/NaB[3H]4 and by cytofluorometry with the monoclonal antibody to Gb3 (38.13). Tritium 276-278 alpha 1,4-galactosyltransferase (P blood group) Homo sapiens 141-144 2402522-4 1990 A new simple technique for separating protein bound [3H]PGF2 alpha (PGF2 alpha receptor-[3H]PGF2 alpha complexes) from free [3H]PGF2 alpha by use of hydroxyapatite (HAP) is introduced. Tritium 89-91 prostaglandin F synthase 2 Bos taurus 56-60 6438103-3 1984 Burkitt lymphoma cell lines (Ramos, Daudi, Put) were characterized by a high chemical quantity of Gb3, high enzyme activity for synthesis of Gb3 (UDP-Gal:LacCer alpha-galactosyltransferase), and a high degree of surface exposure of Gb3, as determined by galactose oxidase/NaB[3H]4 and by cytofluorometry with the monoclonal antibody to Gb3 (38.13). Tritium 276-278 alpha 1,4-galactosyltransferase (P blood group) Homo sapiens 141-144 2402522-4 1990 A new simple technique for separating protein bound [3H]PGF2 alpha (PGF2 alpha receptor-[3H]PGF2 alpha complexes) from free [3H]PGF2 alpha by use of hydroxyapatite (HAP) is introduced. Tritium 89-91 prostaglandin F synthase 2 Bos taurus 68-72 6439814-11 1984 Very small amounts of [3H]IL-2 (less than 1,000 molecules per cell) bind to activated B cells. Tritium 23-25 interleukin 2 Mus musculus 26-30 2402522-4 1990 A new simple technique for separating protein bound [3H]PGF2 alpha (PGF2 alpha receptor-[3H]PGF2 alpha complexes) from free [3H]PGF2 alpha by use of hydroxyapatite (HAP) is introduced. Tritium 89-91 prostaglandin F synthase 2 Bos taurus 68-72 2402522-4 1990 A new simple technique for separating protein bound [3H]PGF2 alpha (PGF2 alpha receptor-[3H]PGF2 alpha complexes) from free [3H]PGF2 alpha by use of hydroxyapatite (HAP) is introduced. Tritium 89-91 prostaglandin F synthase 2 Bos taurus 68-72 2192706-1 1990 The two peptides calcitonin gene related peptide (CGRP) and amylin at 1 uM levels in an isolated rat diaphragm preparation inhibited insulin stimulated 2-deoxy[3H]glucose transport by 30 and 60 percent, respectively; this was the case at maximal (1 uM) and sub-maximal (0.5 mU) insulin concentrations. Tritium 160-162 islet amyloid polypeptide Rattus norvegicus 60-66 6095896-1 1984 The enkephalin affinity reagent [3H]Tyr-D-Ala-Gly-Phe-Leu-CH2Cl [( 3H]DALECK) was synthesized. Tritium 33-35 proenkephalin Rattus norvegicus 4-14 2163012-2 1990 The IC50 of the C-5 antagonists for the inhibition of [3H]glycine binding closely paralleled their potency both in displacing NMDA-selective L-[3H]glutamate binding and in negatively modulating (+)-[3H]5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imi ne maleate ([3H]MK-801) binding. Tritium 55-57 complement C5 Homo sapiens 16-19 6095896-1 1984 The enkephalin affinity reagent [3H]Tyr-D-Ala-Gly-Phe-Leu-CH2Cl [( 3H]DALECK) was synthesized. Tritium 67-69 proenkephalin Rattus norvegicus 4-14 6333423-6 1984 Labeling of rRNA and tRNA during drug treatment for 24 h with [methyl-3H]Met and [14C]Urd revealed that NPC primarily inhibited RNA methylation, and to a lesser extent, RNA synthesis. Tritium 70-72 NPC intracellular cholesterol transporter 1 Homo sapiens 104-107 1694710-6 1990 Our demonstration of enriched NMDA/glycine-stimulated [3H]TCP binding in stratum radiatum of hippocampal region CA1 but not in cerebellar granule cell layer correlates with electrophysiologic studies that showed NMDA channel ion flux in CA1 but not in cerebellar granule cell layer in adult rats. Tritium 55-57 carbonic anhydrase 1 Rattus norvegicus 112-115 1694710-6 1990 Our demonstration of enriched NMDA/glycine-stimulated [3H]TCP binding in stratum radiatum of hippocampal region CA1 but not in cerebellar granule cell layer correlates with electrophysiologic studies that showed NMDA channel ion flux in CA1 but not in cerebellar granule cell layer in adult rats. Tritium 55-57 carbonic anhydrase 1 Rattus norvegicus 237-240 6388574-3 1984 These intact hypothalamic cells did not degrade GnRH during 4 hours of incubation, but a 50% degradation occurred after 24 hours incubation followed by HPLC using specifically tritium labeled GnRH or RIA. Tritium 176-183 gonadotropin releasing hormone 1 Rattus norvegicus 192-196 2335274-4 1990 Oral administration of 3H-labeled retinol 3 h before surgery resulted in 350% more tritium in the serum retinol-RBP-TTR complex of rats with acute renal failure as compared to sham-operated rats; this increase represented the fraction of retinol in RBP-TTR contributed by hepatic retinol from newly absorbed 3H-labeled retinol. Tritium 23-25 retinol binding protein 4 Rattus norvegicus 112-115 6510472-1 1984 The binding of platelet-activating factor (PAF-acether) to human, rabbit and rat platelets was investigated by incubating washed platelets at 37 degrees C with radiolabeled [3H]PAF-acether. Tritium 174-176 PCNA clamp associated factor Homo sapiens 43-46 2335274-4 1990 Oral administration of 3H-labeled retinol 3 h before surgery resulted in 350% more tritium in the serum retinol-RBP-TTR complex of rats with acute renal failure as compared to sham-operated rats; this increase represented the fraction of retinol in RBP-TTR contributed by hepatic retinol from newly absorbed 3H-labeled retinol. Tritium 23-25 retinol binding protein 4 Rattus norvegicus 249-252 2335274-4 1990 Oral administration of 3H-labeled retinol 3 h before surgery resulted in 350% more tritium in the serum retinol-RBP-TTR complex of rats with acute renal failure as compared to sham-operated rats; this increase represented the fraction of retinol in RBP-TTR contributed by hepatic retinol from newly absorbed 3H-labeled retinol. Tritium 83-90 retinol binding protein 4 Rattus norvegicus 112-115 2373288-6 1990 We found a clear, reversible correlation between proliferation, determined by incorporation of [3H]-TdR, and induction of vimentin in the luminal epithelial cells. Tritium 96-98 vimentin Homo sapiens 122-130 1968961-1 1990 [3H]Dynorphin A(1-8) is readily metabolised by rat lumbosacral spinal cord tissue in vitro, affording a variety of products including a significant amount (20% recovered activity) of [3H][Leu5]enkephalin. Tritium 1-3 proenkephalin Rattus norvegicus 193-203 1968961-1 1990 [3H]Dynorphin A(1-8) is readily metabolised by rat lumbosacral spinal cord tissue in vitro, affording a variety of products including a significant amount (20% recovered activity) of [3H][Leu5]enkephalin. Tritium 184-186 proenkephalin Rattus norvegicus 193-203 6510472-5 1984 Addition of unlabeled PAF-acether 5 min after the addition of [3H]PAF-acether showed that internalization of 66 +/- 9% of the specific binding in human and 52 +/- 6.9% in rabbit platelets had occurred. Tritium 63-65 PCNA clamp associated factor Homo sapiens 22-25 6510472-5 1984 Addition of unlabeled PAF-acether 5 min after the addition of [3H]PAF-acether showed that internalization of 66 +/- 9% of the specific binding in human and 52 +/- 6.9% in rabbit platelets had occurred. Tritium 63-65 PCNA clamp associated factor Homo sapiens 66-69 6510472-6 1984 Specific binding of [3H]PAF-acether in rabbit platelets correlated well with [3H]serotonin release in response to different doses of PAF-acether and with the uptake of calcium by the platelets. Tritium 21-23 PCNA clamp associated factor Homo sapiens 24-27 2156021-2 1990 In this report, the binding characteristics of [3H]DPDPE in 5-day-old neonatal (P-5) and adult rat brain are compared. Tritium 48-50 protein disulfide isomerase family A, member 6 Rattus norvegicus 80-83 6510472-6 1984 Specific binding of [3H]PAF-acether in rabbit platelets correlated well with [3H]serotonin release in response to different doses of PAF-acether and with the uptake of calcium by the platelets. Tritium 21-23 PCNA clamp associated factor Homo sapiens 133-136 6510472-6 1984 Specific binding of [3H]PAF-acether in rabbit platelets correlated well with [3H]serotonin release in response to different doses of PAF-acether and with the uptake of calcium by the platelets. Tritium 78-80 PCNA clamp associated factor Homo sapiens 24-27 2158549-2 1990 [3H]Inositol-I-PO4 accumulation during a 60-min exposure to an alpha-1 or alpha-2 agonist in the presence of 5 mM LiCl was used as an index of phosphatidylinositol-4,5-bisphosphate hydrolysis. Tritium 1-3 adrenoceptor alpha 1D Homo sapiens 63-70 6510472-6 1984 Specific binding of [3H]PAF-acether in rabbit platelets correlated well with [3H]serotonin release in response to different doses of PAF-acether and with the uptake of calcium by the platelets. Tritium 78-80 PCNA clamp associated factor Homo sapiens 133-136 6090098-4 1984 The purified [3H]SCF had a specific activity of approximately 20,000 dpm/micrograms protein and was biologically active in pituitary cell cultures. Tritium 14-16 KIT ligand Rattus norvegicus 17-20 6090098-6 1984 The binding was optimal at pH 7.4 and was decreased by pretreatment of [3H]SCF with trypsin (0.25%; 37 C; 30 min) or heat (100 C; 10 min). Tritium 72-74 KIT ligand Rattus norvegicus 75-78 2306727-6 1990 The analogues were as potent as their parent drugs in inhibiting DHFR activity in vitro and at displacing [3H]MTX from intracellular DHFR. Tritium 107-109 dihydrofolate reductase Homo sapiens 133-137 6090098-8 1984 Over 90% of the bound [3H]SCF was competitively inhibited by excess unlabeled SCF and several other inhibin preparations of testicular origin: high mol wt fraction of ram testis fluid (mol wt, greater than 10,000), low mol wt fraction of ram testis fluid (mol wt, less than 5,000), ovine testicular lymph, and aqueous rat testicular extract. Tritium 23-25 KIT ligand Rattus norvegicus 26-29 6090098-8 1984 Over 90% of the bound [3H]SCF was competitively inhibited by excess unlabeled SCF and several other inhibin preparations of testicular origin: high mol wt fraction of ram testis fluid (mol wt, greater than 10,000), low mol wt fraction of ram testis fluid (mol wt, less than 5,000), ovine testicular lymph, and aqueous rat testicular extract. Tritium 23-25 KIT ligand Rattus norvegicus 78-81 2322233-2 1990 The bulk of the bound [3H]-PAF eluted with a higher molecular weight species of PCBP, possibly an aggregated form of PCBP. Tritium 23-25 PCNA clamp associated factor Homo sapiens 27-30 6090502-9 1984 Both IGF-I and IGF-II stimulated [3H]glucose incorporation over the range of 10 pM-10 nM; IGF-I was three to fivefold more potent. Tritium 34-36 insulin like growth factor 2 Homo sapiens 15-21 2322233-3 1990 A smaller amount of [3H]-PAF co-eluted with the major monomeric species of PCBP. Tritium 21-23 PCNA clamp associated factor Homo sapiens 25-28 2157740-2 1990 By means of in vitro receptor autoradiography, NPY binding sites in primate and feline visual cortex were specifically labeled with 3H-NPY. Tritium 132-134 neuropeptide Y Homo sapiens 47-50 6237643-1 1984 Intact, thioglycollate-stimulated murine macrophages cultured on an insoluble [3H]-elastin substratum progressively hydrolysed the elastin. Tritium 79-81 elastin Mus musculus 83-90 2157740-2 1990 By means of in vitro receptor autoradiography, NPY binding sites in primate and feline visual cortex were specifically labeled with 3H-NPY. Tritium 132-134 neuropeptide Y Homo sapiens 135-138 2158852-5 1990 The preferential mu-receptor agonist [D-Ala2,N-methyl-Phe4,Gly-ol5]enkephalin also reduced [3H]acetylcholine release in all groups, but only at the high concentration of 10 micromols/l. Tritium 92-94 proenkephalin Rattus norvegicus 67-77 6237643-1 1984 Intact, thioglycollate-stimulated murine macrophages cultured on an insoluble [3H]-elastin substratum progressively hydrolysed the elastin. Tritium 79-81 elastin Mus musculus 131-138 2156437-6 1990 Actively growing cells, defined by [3H]thymidine incorporations, demonstrated an amiloride-sensitive Na(+)-dependent pHi recovery after an acid load. Tritium 36-38 glucose-6-phosphate isomerase Rattus norvegicus 117-120 6487273-1 1984 The incorporation and metabolism of both vesicle- and LDL (low-density lipoprotein)- derived [3H]cholesterol by LDL-receptor-negative fibroblasts were studied. Tritium 94-96 low density lipoprotein receptor Homo sapiens 112-124 6093016-1 1984 The distribution of opioid receptors in the stomach has been studied using autoradiography of slide-mounted tissue sections incubated with [3H]D-Ala2,D-Leu5-enkephalin. Tritium 140-142 proenkephalin Rattus norvegicus 157-167 6540247-6 1984 DMSO-treated P-29 cells degraded [3H]leucine-labelled subendothelial matrix much more than did untreated cells. Tritium 34-36 SYF2 homolog, RNA splicing factor (S. cerevisiae) Mus musculus 13-17 6481248-3 1984 This peak was well separated from other classes of phospholipid standards including 2-lysophosphatidylcholine and 3H-labeled lyso-PAF. Tritium 114-116 PCNA clamp associated factor Homo sapiens 130-133 6202699-12 1984 In muscle conditioned medium, myotendinous antigen is noncovalently complexed to very high molecular weight material that could be heavily labeled by [3H]glucosamine and [35S]sulfate. Tritium 151-153 tenascin C Gallus gallus 30-50 6206436-2 1984 Substance K and neuromedin K, two newly isolated substance P-related mammalian peptides, are very weak competitors for [3H]substance P binding sites. Tritium 120-122 tachykinin precursor 3 Homo sapiens 16-28 6235299-4 1984 E1,E2 and E3 produced were decided by submission to extraction with ethyl acetate, Bio-Rad AG1-X2 resin column chromatography, thin layer chromatography and then co-crystallization to constant specific activity and the 3H/14C ratio. Tritium 219-221 small nucleolar RNA, H/ACA box 73A Homo sapiens 0-12 6235299-5 1984 The yield of isolated E1,E2 and E3 were calculated from the 3H/14C ratio of the final crystal. Tritium 60-62 small nucleolar RNA, H/ACA box 73A Homo sapiens 22-34 6377112-5 1984 Determination of GnRH cleavage sites was performed by amino acid analysis of the fragments obtained after incubation of the peptidase with (3H)-GnRH labelled on the pyroglutamic acid residue, in presence of carboxypeptidase and peptidyldipeptidase inhibitors. Tritium 140-142 gonadotropin releasing hormone 1 Rattus norvegicus 17-21 6377112-5 1984 Determination of GnRH cleavage sites was performed by amino acid analysis of the fragments obtained after incubation of the peptidase with (3H)-GnRH labelled on the pyroglutamic acid residue, in presence of carboxypeptidase and peptidyldipeptidase inhibitors. Tritium 140-142 gonadotropin releasing hormone 1 Rattus norvegicus 144-148 6733083-4 1984 Stoichiometry determinations using [3H]actin and [14C]subfragment 1 indicate that the major doublet is a 1:1 complex of actin and subfragment 1. Tritium 36-38 actin Oryctolagus cuniculus 120-125 6726182-6 1984 In infected cells, the counterparts to E1 and E2 were labelled with [3H]mannose and both migrated in gels as sharp bands, indicating that the heterogeneity observed in virion E2 was produced during virus maturation. Tritium 69-71 small nucleolar RNA, H/ACA box 73A Homo sapiens 39-48 6707203-3 1984 Alb was labeled with tritium by reductive methylation ( [3H3C]Alb). Tritium 21-28 albumin Oryctolagus cuniculus 0-3 6707203-3 1984 Alb was labeled with tritium by reductive methylation ( [3H3C]Alb). Tritium 21-28 albumin Oryctolagus cuniculus 62-65 6619145-10 1983 Elution profiles of ERN on HAP chromatography reveal 2 different binding components at 30 min and at 6 h of continuous [3H]E2 exposure. Tritium 120-122 estrogen receptor 1 Rattus norvegicus 20-23 6668616-5 1983 At all ip doses (1, 10, and 100 mg/kg), rapid and extensive metabolism of 3H-labeled 2,6-DNT was observed, as judged by the low amounts (less than 2% of the total 3H/tissue) of unchanged 3H-labeled 2,6-DNT that could be reisolated from blood, liver, small intestine, or lungs at 2 h after administration. Tritium 74-76 5',3'-nucleotidase, cytosolic Mus musculus 89-92 6668616-5 1983 At all ip doses (1, 10, and 100 mg/kg), rapid and extensive metabolism of 3H-labeled 2,6-DNT was observed, as judged by the low amounts (less than 2% of the total 3H/tissue) of unchanged 3H-labeled 2,6-DNT that could be reisolated from blood, liver, small intestine, or lungs at 2 h after administration. Tritium 74-76 5',3'-nucleotidase, cytosolic Mus musculus 202-205 6668616-5 1983 At all ip doses (1, 10, and 100 mg/kg), rapid and extensive metabolism of 3H-labeled 2,6-DNT was observed, as judged by the low amounts (less than 2% of the total 3H/tissue) of unchanged 3H-labeled 2,6-DNT that could be reisolated from blood, liver, small intestine, or lungs at 2 h after administration. Tritium 163-165 5',3'-nucleotidase, cytosolic Mus musculus 89-92 6668616-5 1983 At all ip doses (1, 10, and 100 mg/kg), rapid and extensive metabolism of 3H-labeled 2,6-DNT was observed, as judged by the low amounts (less than 2% of the total 3H/tissue) of unchanged 3H-labeled 2,6-DNT that could be reisolated from blood, liver, small intestine, or lungs at 2 h after administration. Tritium 163-165 5',3'-nucleotidase, cytosolic Mus musculus 89-92 6668616-6 1983 The extent of 3H-labeled 2,6-DNT metabolism by these tissues after po administration was similar, except for the relatively high amounts of unchanged 3H-labeled 2,6-DNT (approximately 50% of the total 3H/tissue between 1 and 8 h after 100 mg/kg) present in the small intestine. Tritium 14-16 5',3'-nucleotidase, cytosolic Mus musculus 29-32 6668616-6 1983 The extent of 3H-labeled 2,6-DNT metabolism by these tissues after po administration was similar, except for the relatively high amounts of unchanged 3H-labeled 2,6-DNT (approximately 50% of the total 3H/tissue between 1 and 8 h after 100 mg/kg) present in the small intestine. Tritium 150-152 5',3'-nucleotidase, cytosolic Mus musculus 165-168 6668616-6 1983 The extent of 3H-labeled 2,6-DNT metabolism by these tissues after po administration was similar, except for the relatively high amounts of unchanged 3H-labeled 2,6-DNT (approximately 50% of the total 3H/tissue between 1 and 8 h after 100 mg/kg) present in the small intestine. Tritium 150-152 5',3'-nucleotidase, cytosolic Mus musculus 165-168 6603985-4 1983 Anti-CSF neutralized the HSA effect on species-specific marrow cell [3H] TdR incorporation relative to the effects of normal sera with an efficiency of anti-CSF produced graded neutralization of the human HSA effect on human marrow cell proliferation. Tritium 69-71 colony stimulating factor 2 Homo sapiens 5-8 6884443-2 1983 More than 90% of these spleen cells are recognizable erythroid and form the basis of a new in vitro microassay for erythropoietin (Ep) which uses 3H-thymidine incorporation as an endpoint. Tritium 146-148 erythropoietin Mus musculus 115-129 6884428-1 1983 (-)-N-(2-Chloroethyl)-norapomorphine [-)-NCA) inhibited in a concentration-dependent manner the electrically evoked [3H]acetylcholine release in slices of cat caudate. Tritium 117-119 CEA cell adhesion molecule 6 Homo sapiens 41-44 6884428-3 1983 Although (-)-NCA is an irreversible antagonist at some behaviorally relevant postsynaptic dopamine receptors, its effect as an agonist on dopamine receptors modulating [3H]acetylcholine release strongly resembles its action on presynaptic dopamine autoreceptors modulating [3H]dopamine release. Tritium 169-171 CEA cell adhesion molecule 6 Homo sapiens 13-16 6884428-3 1983 Although (-)-NCA is an irreversible antagonist at some behaviorally relevant postsynaptic dopamine receptors, its effect as an agonist on dopamine receptors modulating [3H]acetylcholine release strongly resembles its action on presynaptic dopamine autoreceptors modulating [3H]dopamine release. Tritium 274-276 CEA cell adhesion molecule 6 Homo sapiens 13-16 6304248-8 1983 The cross-competition IC50 data supported this conclusion, showing a loss in the potency of morphine in displacing [3H]DADL enkephalin in the thalamus of MSG-treated rats. Tritium 116-118 proenkephalin Rattus norvegicus 124-134 6306069-4 1983 By contrast, opiate receptors labeled with [3H]D-Ala2-D-Leu5-enkephalin in a delta ligand selectivity pattern are much more homogeneously distributed across both regions and laminae within regions. Tritium 44-46 proenkephalin Rattus norvegicus 61-71 6306069-13 1983 Although regional variations in [3H]D-Ala2-D-Leu5-enkephalin-labeled receptor density are uncommon, a gradual decrease in the number of sites along the dorsomedial wall of the cortex from anterior cingulate to caudal retrosplenial limbic cortex can be observed. Tritium 33-35 proenkephalin Rattus norvegicus 50-60 6133596-2 1983 The dose-response curves show that IGF I is more potent than insulin; half maximal stimulation of [3H]thymidine incorporation is obtained at about 0.4 nM IGF I and 14 nM insulin, respectively. Tritium 99-101 insulin-like growth factor 1 Rattus norvegicus 35-40 6133596-2 1983 The dose-response curves show that IGF I is more potent than insulin; half maximal stimulation of [3H]thymidine incorporation is obtained at about 0.4 nM IGF I and 14 nM insulin, respectively. Tritium 99-101 insulin-like growth factor 1 Rattus norvegicus 154-159 6339562-6 1983 Insulin over a concentration range of 10 ng/ml-10 microgram/ml, stimulated [3H]thymidine incorporation into the DNA of retinal pericytes, and endothelial cells and aortic smooth muscle cells but had no effect on aortic endothelial cells. Tritium 76-78 insulin Bos taurus 0-7 6572590-1 1983 In the presence of thiorphan an "enkephalinase" inhibitor, bestatin an aminopeptidase inhibitor of bacterial origin potently inhibited the hydrolysis of [3H][Leu5]enkephalin by slices from rat striatum with an IC50 value of about 0.2 microM whereas puromycin was approximately 1000 times less potent on this preparation. Tritium 154-156 proenkephalin Rattus norvegicus 33-43 6572590-2 1983 In vivo bestatin or thiorphan (but not puromycin) significantly protected [3H][Met5]enkephalin administered intracerebroventricularly to mice from hydrolysis and co-administration of these two peptidase inhibitors resulted in a strong reduction in the appearance of hydrolysis products in brain. Tritium 75-77 proenkephalin Rattus norvegicus 84-94 6313708-6 1983 FSH-sensitive adenylate cyclase activity, as measured by the conversion of (3H)-ATP to (3H)-cAMP, was inhibited by GnRHa and EGF at 48 h of culture. Tritium 75-80 gonadotropin releasing hormone 1 Rattus norvegicus 115-120 6319864-1 1983 Pretreatment of rat brain membranes at pH 4.5 increased GTP and Gpp(NH)p regulation of [3H]-D-ala2-met5-enkephalinamide (D-ala-enk) binding with no change in absolute binding itself. Tritium 88-90 proenkephalin Rattus norvegicus 104-107 6126526-6 1982 [3H]Spiperone binds to high-affinity sites in homogenates of nucleus accumbens, which are likely to be D-2 receptors. Tritium 1-3 solute carrier family 3 member 1 Rattus norvegicus 103-106 6982901-5 1982 Trifluoperazine, W-7, cytochalasin D, and taxol also block DNA synthesis in response to EGF as measured by autoradiography using [3H]thymidine. Tritium 130-132 epidermal growth factor Homo sapiens 88-91 6292632-4 1982 Conversely, beta-endorphin inhibited the binding of a series of 3H-labeled ligands, including dihydromorphine, ethylketocyclazocine, SKF 10,047, naloxone and D-ala2-D-leu5-enkephalin, far less potently than their corresponding unlabeled drug. Tritium 64-66 proenkephalin Rattus norvegicus 172-182 6812655-3 1982 Elution profiles of [3H]AA and metabolites in extracts of culture medium revealed that 13, 14-dihydro-15-keto-PGF2 alpha (PGFM), Pge2, PGF2 alpha, and at least four unidentified compounds were produced by Day 16 and Day 19 blastocysts. Tritium 21-23 prostaglandin F synthase 2 Bos taurus 110-114 6812655-9 1982 The third experiment was conducted to determine directly if Day 19 blastocysts and endometrial slices metabolized [3H]PGF2 alpha to [3H]PGFM. Tritium 115-117 prostaglandin F synthase 2 Bos taurus 118-122 6812655-11 1982 Endometrial slices metabolized 34.3 +/- 1.5% of the [3H]PGF2 alpha to [3H]PGFM, while blastocysts metabolized 7.5 +/- 1.6% of the [3H]PGF2 alpha to [3H]PGFM. Tritium 53-55 prostaglandin F synthase 2 Bos taurus 56-60 6812655-11 1982 Endometrial slices metabolized 34.3 +/- 1.5% of the [3H]PGF2 alpha to [3H]PGFM, while blastocysts metabolized 7.5 +/- 1.6% of the [3H]PGF2 alpha to [3H]PGFM. Tritium 71-73 prostaglandin F synthase 2 Bos taurus 56-60 6812655-11 1982 Endometrial slices metabolized 34.3 +/- 1.5% of the [3H]PGF2 alpha to [3H]PGFM, while blastocysts metabolized 7.5 +/- 1.6% of the [3H]PGF2 alpha to [3H]PGFM. Tritium 71-73 prostaglandin F synthase 2 Bos taurus 56-60 6812655-11 1982 Endometrial slices metabolized 34.3 +/- 1.5% of the [3H]PGF2 alpha to [3H]PGFM, while blastocysts metabolized 7.5 +/- 1.6% of the [3H]PGF2 alpha to [3H]PGFM. Tritium 71-73 prostaglandin F synthase 2 Bos taurus 56-60 7104393-6 1982 Ligand specificity studies (using [3H]R 2858 as well as [3H]estradiol-17 beta) showed that R 2858 binds to an estrogen receptor in rat skeletal muscle. Tritium 35-37 estrogen receptor 1 Rattus norvegicus 110-127 6286693-1 1982 The level of occupancy of a single class of high-affinity (3H)-phorbol 12, 13-dibutyrate (PDBu) binding sites (Kd = 26 nM) in quiescent Swiss 3T3 cells was correlated with the dose of PDBu required to stimulate rapid (increase in 86Rb uptake, decrease in epidermal growth factor receptor affinity) and long-term (induction of 2-deoxyglucose uptake, initiation of DNA synthesis) events of the proliferative response. Tritium 59-61 epidermal growth factor receptor Mus musculus 255-287 6283022-7 1982 Dimethylaminonaphtylsulfonyl (dansyl) derivatives of Met- and Leu-enkephalin correspond to [3H]dansyl derivatives of Met-like substances from hybrid cells. Tritium 92-94 prodynorphin Homo sapiens 62-76 6287338-3 1982 A micro-dissection of coronal sections of guinea-pig hippocampus revealed that the specific binding capacity for [3H]CHA in area CA-1 was 20-30% higher than in area CA-3, dentate gyrus and subiculum. Tritium 114-116 carbonic anhydrase 1 Cavia porcellus 129-133 7037785-1 1982 Aspartate beta-decarboxylase catalyzes abortive decarboxylation/transamination of [2-3H]aspartate with at least 17% internal transfer of tritium to the pro-S position at C-4" of the resulting pyridoxamine phosphate. Tritium 137-144 complement C4A (Rodgers blood group) Homo sapiens 170-173 6280806-4 1982 Moreover, both morphine and naloxone were equally active in competing for [3H]Met-Enk binding in all 3 brain regions during the first month after birth, whereas methionine-enkephalin was only about 30-50% as active as morphine n competing for [3H]naloxone binding in that period. Tritium 75-77 proenkephalin Rattus norvegicus 82-85 6953412-3 1982 Furthermore, in the presence of Mg2+, eIF-2-SP catalyzes the exchange of eIF-2-bound [3H]GDP with unlabeled GDP or GTP (GDP exchange) and the release of [3H]GDP when the ternary complex is formed from eIF-2-[3H]GDP, GTP, and [35S]Met-tRNAi. Tritium 154-156 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 38-43 6953412-3 1982 Furthermore, in the presence of Mg2+, eIF-2-SP catalyzes the exchange of eIF-2-bound [3H]GDP with unlabeled GDP or GTP (GDP exchange) and the release of [3H]GDP when the ternary complex is formed from eIF-2-[3H]GDP, GTP, and [35S]Met-tRNAi. Tritium 154-156 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 73-78 6283696-1 1982 Tritium levels in tissues of rats were determined after inhalation of tritiated benzo(a)pyrene (3H-BaP; 500 micrograms/l; activity mass median diameter, 1-2 micrometers) for 1 h. Significant amounts of radioactivity were found in the nasal turbinates, trachea, larynx, lungs, tracheobronchial lymph nodes, kidneys, and liver immediately after exposure. Tritium 0-7 prohibitin 2 Rattus norvegicus 99-102 7062117-6 1982 Synthesis of [3H]ACh was detectable on days E10 to E12 but increased markedly between days E13 and E14. Tritium 14-16 skull morphology 19 Mus musculus 91-94 7062117-7 1982 Uptake and radioautographic labeling by 5-[3H]HT was seen first in the foregut on day E12, in the colon on day E13, and in the terminal colon on day E14. Tritium 43-45 skull morphology 19 Mus musculus 111-114 6172502-5 1982 T lymphocytes or interleukin 2 (IL-2) of young or aged rats augment the uptake of 3H-TdR in cultures of "young" B cells responding to anti-Ig reagents or LPS and DxS, but have no significant effect on the responses of "old" B cells. Tritium 82-84 interleukin 2 Rattus norvegicus 17-30 6172502-5 1982 T lymphocytes or interleukin 2 (IL-2) of young or aged rats augment the uptake of 3H-TdR in cultures of "young" B cells responding to anti-Ig reagents or LPS and DxS, but have no significant effect on the responses of "old" B cells. Tritium 82-84 interleukin 2 Rattus norvegicus 32-36 7055686-1 1982 Dopamine-beta-hydroxylase activity can be assayed in vitro in suspensions of rat hypothalamic synaptosomes by following tritium release from [2-3H]dopamine in the presence of monoamine oxidase inhibitors. Tritium 120-127 dopamine beta-hydroxylase Rattus norvegicus 0-25 6125567-1 1982 The uptake and metabolism of H-Pro-[3H]Leu-Gly-NH2 ([3H]PLG) in rat brain was investigated by reverse-phase paired-ion high pressure liquid chromatography. Tritium 36-38 plasminogen Rattus norvegicus 56-59 6125567-2 1982 Following in vitro incubation of [3H]PLG with rat brain subcellular preparations, the microsomal-cytosol fraction was about twice as active in degrading PLG as the crude mitochondrial-synaptosomal fraction. Tritium 34-36 plasminogen Rattus norvegicus 37-40 6125567-2 1982 Following in vitro incubation of [3H]PLG with rat brain subcellular preparations, the microsomal-cytosol fraction was about twice as active in degrading PLG as the crude mitochondrial-synaptosomal fraction. Tritium 34-36 plasminogen Rattus norvegicus 153-156 6125567-5 1982 After intravenous injection of [3H]PLG the uptake of unmetabolized peptide in the brain appeared to be very low: 0.008% and 0.001% of the administered dose/g tissue at 2 and 5 min after injection respectively, while at longer survival times intact peptide was below the detection limit. Tritium 32-34 plasminogen Rattus norvegicus 35-38 6125567-6 1982 Compared with the intravenous route of administration, intracerebroventricular injection of [3H]PLG yielded much higher brain concentrations of unmetabolized PLG. Tritium 93-95 plasminogen Rattus norvegicus 96-99 6125567-6 1982 Compared with the intravenous route of administration, intracerebroventricular injection of [3H]PLG yielded much higher brain concentrations of unmetabolized PLG. Tritium 93-95 plasminogen Rattus norvegicus 158-161 6125567-8 1982 However, the in vivo experiments also showed considerable incorporation of [3H]leucine liberated from [3H]PLG into proteins. Tritium 76-78 plasminogen Rattus norvegicus 106-109 7325230-0 1981 Uptake of [3H]PAH and [14C]urate into isolated proximal tubular segments of the pig kidney. Tritium 11-13 phenylalanine hydroxylase Sus scrofa 14-17 7325230-8 1981 In inhibitor studies urate significantly depressed the uptake of [3H]PAH, and unlabeled PAH depressed the uptake of [14C]urate. Tritium 66-68 phenylalanine hydroxylase Sus scrofa 69-72 7325230-10 1981 These results suggest that [14C]urate and [3H]PAH are transported by a transport system located mainly in the proximal convoluted tubule. Tritium 43-45 phenylalanine hydroxylase Sus scrofa 46-49 7262029-3 1981 The activity induced in the absence of exogenous prolactin corresponds to newly synthesized alpha-lactalbumin molecules, since 1) the activity is suppressed completely by rabbit anti-rat alpha-lactalbumin serum and 2) culture in the presence of [3H]-amino acids generates [3H]-alpha-lactalbumin, identified by SDS-polyacrylamide gel electrophoresis. Tritium 246-248 lactalbumin, alpha Rattus norvegicus 92-109 7262029-3 1981 The activity induced in the absence of exogenous prolactin corresponds to newly synthesized alpha-lactalbumin molecules, since 1) the activity is suppressed completely by rabbit anti-rat alpha-lactalbumin serum and 2) culture in the presence of [3H]-amino acids generates [3H]-alpha-lactalbumin, identified by SDS-polyacrylamide gel electrophoresis. Tritium 246-248 lactalbumin, alpha Rattus norvegicus 187-204 7262029-3 1981 The activity induced in the absence of exogenous prolactin corresponds to newly synthesized alpha-lactalbumin molecules, since 1) the activity is suppressed completely by rabbit anti-rat alpha-lactalbumin serum and 2) culture in the presence of [3H]-amino acids generates [3H]-alpha-lactalbumin, identified by SDS-polyacrylamide gel electrophoresis. Tritium 246-248 lactalbumin, alpha Rattus norvegicus 187-204 7262029-3 1981 The activity induced in the absence of exogenous prolactin corresponds to newly synthesized alpha-lactalbumin molecules, since 1) the activity is suppressed completely by rabbit anti-rat alpha-lactalbumin serum and 2) culture in the presence of [3H]-amino acids generates [3H]-alpha-lactalbumin, identified by SDS-polyacrylamide gel electrophoresis. Tritium 273-275 lactalbumin, alpha Rattus norvegicus 92-109 6264807-3 1981 GnRH significantly (P less than 0.01) increased both incorporation of [3H]glucosamine into LH (glycosylation) and release of immunoreactive LH (IR-LH) and [3H]LH. Tritium 71-73 gonadotropin releasing hormone 1 Rattus norvegicus 0-4 6264807-6 1981 Release of [3H]LH was increased by GnRH, 8-Br-cAMP, and DBcAMP, but not by other nucleotides. Tritium 12-14 gonadotropin releasing hormone 1 Rattus norvegicus 35-39 7012154-3 1981 Upon aromatization of the above material by placental microsomal aromatase preparation, a process involving 1 beta hydrogen elimination, only 7.4% of the isotope was lost establishing the alpha orientation of the 3H at C-1 in the substrate. Tritium 213-215 heterogeneous nuclear ribonucleoprotein C Homo sapiens 219-222 7237512-4 1981 These mean TG2 values were much shorter than expected from [3H]TdR double labelling experiments and from a multiparameter cell kinetic study in hairless mouse epidermis and did not reflect the circadian variations seen in these studies. Tritium 60-62 transglutaminase 2, C polypeptide Mus musculus 11-14 7195354-3 1981 Progesterone receptor was determined in purified endometrial nuclei by Scatchard analysis of the specific binding of 3H-R5020 under exchange conditions. Tritium 117-119 progesterone receptor Oryctolagus cuniculus 0-21 10250470-2 1981 The NRC is amending its regulations to permit licensees greater leeway in disposing of liquid scintillation media and animal carcasses containing tracer levels of hydrogen-3 (tritium) or carbon-14. Tritium 175-182 nuclear receptor coactivator 6 Homo sapiens 4-7 7016968-4 1981 However, the amount of 3H-LHRH incorporated in the pituitary gland of rats bearing different types of constant estrous-anovulatory syndrome did not present significant variations in relation to the procedures employed in sterilization. Tritium 23-25 gonadotropin releasing hormone 1 Rattus norvegicus 26-30 6257844-7 1981 A similar fixation seems to occur on brain membranes after [3H]SAH administration to rat. Tritium 60-62 acyl-CoA synthetase medium-chain family member 3 Rattus norvegicus 63-66 7193615-3 1980 Progesterone receptor was determined in purified endometrial nuclei by Scatchard analysis of the specific binding of 3H-R5020 under exchange conditions. Tritium 117-119 progesterone receptor Oryctolagus cuniculus 0-21 6998827-2 1980 After a single injection of [3H]thymidine, the labeling index of gastrin cells was 0.3%, indicating that a few gastrin cells at the isthmus region have a self-replicating activity. Tritium 29-31 gastrin Homo sapiens 65-72 2303535-5 1990 Third, following simultaneous intracarotid injections of [3H]DG and [14C]glucose, the ratio K1/K1 was 1.1 in glioma grafts and 1.3 in left hemisphere. Tritium 58-60 keratin 1 Rattus norvegicus 92-105 6998827-2 1980 After a single injection of [3H]thymidine, the labeling index of gastrin cells was 0.3%, indicating that a few gastrin cells at the isthmus region have a self-replicating activity. Tritium 29-31 gastrin Homo sapiens 111-118 2306629-2 1990 When treated with the conditioned medium of L929 mouse fibroblast cells as a crude CSF-1 (mouse macrophage-colony stimulating factor) or purified CSF-1, microglia showed morphological changes and increased in both cell number and [3H]thymidine uptake. Tritium 231-233 colony stimulating factor 1 (macrophage) Mus musculus 146-151 7305913-9 1980 Degradation of glucose from glycogen labelled by 3H2O showed that most of the 3H was located at C-2 and C-5. Tritium 49-51 complement C2 Rattus norvegicus 96-99 2306559-2 1990 Maximal effect of aFGF and EGF on DNA synthesis measured by [3H]thymidine incorporation was observed after 18 h. aFGF stimulated DNA synthesis by 3.5-fold with an ED50 of 0.75 ng/ml while a 2.3-fold EGF stimulation was recorded with an ED50 of 0.067 ng/ml. Tritium 61-63 fibroblast growth factor 1 Rattus norvegicus 18-22 2306559-2 1990 Maximal effect of aFGF and EGF on DNA synthesis measured by [3H]thymidine incorporation was observed after 18 h. aFGF stimulated DNA synthesis by 3.5-fold with an ED50 of 0.75 ng/ml while a 2.3-fold EGF stimulation was recorded with an ED50 of 0.067 ng/ml. Tritium 61-63 fibroblast growth factor 1 Rattus norvegicus 113-117 1688805-5 1990 Autoradiographic experiments revealed that not all the cells presenting an increase in p55 incorporated [3H]thymidine, indicating that this protein is not related to DNA replication. Tritium 105-107 MAGUK p55 scaffold protein 1 Rattus norvegicus 87-90 2153409-6 1990 Assays performed with [3H]PI and [3H]PIP substrates in the presence of 500 microM [3H]Gro-PIP revealed approx. Tritium 23-25 growth-regulated alpha protein Cavia porcellus 86-89 2153409-6 1990 Assays performed with [3H]PI and [3H]PIP substrates in the presence of 500 microM [3H]Gro-PIP revealed approx. Tritium 34-36 growth-regulated alpha protein Cavia porcellus 86-89 2153409-6 1990 Assays performed with [3H]PI and [3H]PIP substrates in the presence of 500 microM [3H]Gro-PIP revealed approx. Tritium 34-36 growth-regulated alpha protein Cavia porcellus 86-89 2153409-7 1990 75% less [3H]inositol 1-phosphate ([3H]IP1) and [3H]inositol 1,4-bisphosphate ([3H]IP2) product formation, respectively, indicating that [3H]Gro-PIP inhibited the hydrolysis of the substrates by PI-PLC I. Tritium 10-12 growth-regulated alpha protein Cavia porcellus 141-144 6252249-2 1980 IGF I caused a dose-dependent stimulation of the incorporation of [3H]thymidine into DNA at concentrations of 0.1--100 nM; the effect appeared after 6 h, was maximal at 12 h, and was sustained for 96 h. IGF I also increased the bone DNA content, IGF I at 0.1--3 nM had a small stimulatory effect on the incorporation of [3H]proline into collagenase-digestible protein (CDP) whereas 30 nM IGF I caused a two- to threefold increment and had a maximal effect. Tritium 67-69 insulin-like growth factor 1 Rattus norvegicus 0-5 6252249-2 1980 IGF I caused a dose-dependent stimulation of the incorporation of [3H]thymidine into DNA at concentrations of 0.1--100 nM; the effect appeared after 6 h, was maximal at 12 h, and was sustained for 96 h. IGF I also increased the bone DNA content, IGF I at 0.1--3 nM had a small stimulatory effect on the incorporation of [3H]proline into collagenase-digestible protein (CDP) whereas 30 nM IGF I caused a two- to threefold increment and had a maximal effect. Tritium 67-69 insulin-like growth factor 1 Rattus norvegicus 203-208 2295617-8 1990 [3H]Phorbol 12,13-dibutyrate binding for PKC I, II, and III was recovered after chromatography; however, the kinase activities of all these enzymes were greatly reduced. Tritium 1-3 protein kinase C iota Homo sapiens 41-59 6252249-2 1980 IGF I caused a dose-dependent stimulation of the incorporation of [3H]thymidine into DNA at concentrations of 0.1--100 nM; the effect appeared after 6 h, was maximal at 12 h, and was sustained for 96 h. IGF I also increased the bone DNA content, IGF I at 0.1--3 nM had a small stimulatory effect on the incorporation of [3H]proline into collagenase-digestible protein (CDP) whereas 30 nM IGF I caused a two- to threefold increment and had a maximal effect. Tritium 67-69 insulin-like growth factor 1 Rattus norvegicus 203-208 6252249-2 1980 IGF I caused a dose-dependent stimulation of the incorporation of [3H]thymidine into DNA at concentrations of 0.1--100 nM; the effect appeared after 6 h, was maximal at 12 h, and was sustained for 96 h. IGF I also increased the bone DNA content, IGF I at 0.1--3 nM had a small stimulatory effect on the incorporation of [3H]proline into collagenase-digestible protein (CDP) whereas 30 nM IGF I caused a two- to threefold increment and had a maximal effect. Tritium 67-69 insulin-like growth factor 1 Rattus norvegicus 203-208 6252249-2 1980 IGF I caused a dose-dependent stimulation of the incorporation of [3H]thymidine into DNA at concentrations of 0.1--100 nM; the effect appeared after 6 h, was maximal at 12 h, and was sustained for 96 h. IGF I also increased the bone DNA content, IGF I at 0.1--3 nM had a small stimulatory effect on the incorporation of [3H]proline into collagenase-digestible protein (CDP) whereas 30 nM IGF I caused a two- to threefold increment and had a maximal effect. Tritium 67-70 insulin-like growth factor 1 Rattus norvegicus 0-5 2306370-1 1990 The effects of surfactant, surfactant lipids, and surfactant protein A (SP-A) on the surface phagocytosis of [3H]thymidine-labeled Staphylococcus aureus (SAE) by rat alveolar macrophages were studied. Tritium 110-112 surfactant protein A2 Homo sapiens 72-76 6252249-2 1980 IGF I caused a dose-dependent stimulation of the incorporation of [3H]thymidine into DNA at concentrations of 0.1--100 nM; the effect appeared after 6 h, was maximal at 12 h, and was sustained for 96 h. IGF I also increased the bone DNA content, IGF I at 0.1--3 nM had a small stimulatory effect on the incorporation of [3H]proline into collagenase-digestible protein (CDP) whereas 30 nM IGF I caused a two- to threefold increment and had a maximal effect. Tritium 67-70 insulin-like growth factor 1 Rattus norvegicus 203-208 6252249-2 1980 IGF I caused a dose-dependent stimulation of the incorporation of [3H]thymidine into DNA at concentrations of 0.1--100 nM; the effect appeared after 6 h, was maximal at 12 h, and was sustained for 96 h. IGF I also increased the bone DNA content, IGF I at 0.1--3 nM had a small stimulatory effect on the incorporation of [3H]proline into collagenase-digestible protein (CDP) whereas 30 nM IGF I caused a two- to threefold increment and had a maximal effect. Tritium 67-70 insulin-like growth factor 1 Rattus norvegicus 203-208 6252249-2 1980 IGF I caused a dose-dependent stimulation of the incorporation of [3H]thymidine into DNA at concentrations of 0.1--100 nM; the effect appeared after 6 h, was maximal at 12 h, and was sustained for 96 h. IGF I also increased the bone DNA content, IGF I at 0.1--3 nM had a small stimulatory effect on the incorporation of [3H]proline into collagenase-digestible protein (CDP) whereas 30 nM IGF I caused a two- to threefold increment and had a maximal effect. Tritium 67-70 insulin-like growth factor 1 Rattus norvegicus 203-208 6252249-9 1980 Cortisol decreased the stimulatory effect of IGF I on DNA labeling but greatly enhanced the stimulatory effect of IGF I on the incorporation of [3H]proline into CDP. Tritium 145-147 insulin-like growth factor 1 Rattus norvegicus 114-119 2241996-2 1990 The tyrosine-alkylating agents, p-nitrobenzenesulphonylfluoride (pNBSF) and tetranitromethane (TNM) caused a time-and dose-dependent loss of the binding of [3H]SCH-23390 and [3H]spiroperidol, ligands specific for dopamine D1 and D2 receptors, respectively. Tritium 157-159 dopamine receptor D1 Bos taurus 213-241 7419667-0 1980 An in vivo tritium release assay of human dopamine beta-hydroxylase. Tritium 11-18 dopamine beta-hydroxylase Homo sapiens 42-67 1689199-1 1990 The autoradiographic distribution of the neurokinin (NK)-3 receptor sub-type was visualized in the rat brain using [3H]senktide, a highly selective ligand, [3H]Senktide apparently binds to a single class of high affinity (Kd = 2.8 +/- 1.0 nM), low capacity (Bmax = 31.2 +/- 3.0 fmol/mg protein) sites in rat brain cortex. Tritium 116-118 tachykinin receptor 3 Rattus norvegicus 41-67 1689199-1 1990 The autoradiographic distribution of the neurokinin (NK)-3 receptor sub-type was visualized in the rat brain using [3H]senktide, a highly selective ligand, [3H]Senktide apparently binds to a single class of high affinity (Kd = 2.8 +/- 1.0 nM), low capacity (Bmax = 31.2 +/- 3.0 fmol/mg protein) sites in rat brain cortex. Tritium 157-159 tachykinin receptor 3 Rattus norvegicus 41-67 6781945-3 1980 FSH increased the incorporation of [3H]testosterone into estradiol during a 3-h incubation of halved pig follicles or isolated granulosa cells by 85% compared to controls. Tritium 36-38 FSH Sus scrofa 0-3 1976450-6 1990 Accumulation of [3H]-VP-16 in H69/VP was 6.1-7.5 times lower than that in H69/P. Tritium 17-19 host cell factor C1 Homo sapiens 21-26 1980888-7 1990 Estimation of radioactivity of [3H]pargyline bound after isoelectric focusing revealed a single protein band with acidic pI values of about 5.5 for rat brain and liver MAO B. Tritium 32-34 monoamine oxidase B Rattus norvegicus 168-173 2262050-5 1990 We found that gastrin, pentagastrin and Gastrin/CCK4 induce ornithine decarboxylase activity, an early event involved in cell proliferation, as well as 3H-thymidine incorporation. Tritium 152-154 gastrin Rattus norvegicus 14-21 2262050-5 1990 We found that gastrin, pentagastrin and Gastrin/CCK4 induce ornithine decarboxylase activity, an early event involved in cell proliferation, as well as 3H-thymidine incorporation. Tritium 152-154 gastrin Rattus norvegicus 40-47 6933539-2 1980 The release of 3H into body water from estradiol labeled with 3H in the 17 alpha, 16 alpha, and C-2 positions permits measurement of the rate and extent of 17 beta-ol oxidation and of the competing hydroxylations at C-2 and 16 alpha, which lead to products with different biologicaly properties. Tritium 15-17 complement C2 Homo sapiens 96-99 2177712-0 1990 Role of adenylate cyclase in modulatory effect of neuropeptide Y on [3H]noradrenaline release in guinea-pig vas deferens. Tritium 69-71 pro-neuropeptide Y Cavia porcellus 50-64 2177712-2 1990 The effect of neuropeptide Y (NPY) on [3H]noradrenaline ([3H]NA) release-evoked by 5-Hz electrical stimulation or 5 microns calcium ionophore A23187 was studied in vitro in guinea-pig vas deferens. Tritium 38-41 pro-neuropeptide Y Cavia porcellus 14-28 6933539-2 1980 The release of 3H into body water from estradiol labeled with 3H in the 17 alpha, 16 alpha, and C-2 positions permits measurement of the rate and extent of 17 beta-ol oxidation and of the competing hydroxylations at C-2 and 16 alpha, which lead to products with different biologicaly properties. Tritium 15-17 complement C2 Homo sapiens 216-219 6933539-2 1980 The release of 3H into body water from estradiol labeled with 3H in the 17 alpha, 16 alpha, and C-2 positions permits measurement of the rate and extent of 17 beta-ol oxidation and of the competing hydroxylations at C-2 and 16 alpha, which lead to products with different biologicaly properties. Tritium 62-64 complement C2 Homo sapiens 96-99 2149373-1 1990 The distribution of estrogen target neurons which contain atrial natriuretic factor (ANF) in female rat hypothalamus was investigated by thaw-mount auto-radiography combined with immunocytochemistry using tritium-labeled estradiol and antibodies against ANF. Tritium 205-212 natriuretic peptide A Rattus norvegicus 85-88 6933542-3 1980 Metabolically labeled [3H]BPgp70 was not immunoprecipitated by IgG-anti RMuLV-gp&) alone, but was immunoprecipitated when gp70 was added, an indicaton of BPgp70 x gp70 complex formation. Tritium 23-25 embigin Mus musculus 28-32 6933542-3 1980 Metabolically labeled [3H]BPgp70 was not immunoprecipitated by IgG-anti RMuLV-gp&) alone, but was immunoprecipitated when gp70 was added, an indicaton of BPgp70 x gp70 complex formation. Tritium 23-25 embigin Mus musculus 126-130 6901615-1 1980 Cultured thioglycollate-elicited mouse peritoneal macrophages secrete an enzyme which hydrolyzes [3H]elastin prepared by NaB3H4 reduction of bovine ligamentum nuchae elastin. Tritium 98-100 elastin Mus musculus 101-108 2381516-4 1990 The serotonin 1C receptor binding sites were visualized autoradiographically and quantified using [3H]mesulergine as ligand, in the presence of spiperone to block serotonin 1C receptors. Tritium 99-102 5-hydroxytryptamine receptor 2C Rattus norvegicus 4-25 6901615-1 1980 Cultured thioglycollate-elicited mouse peritoneal macrophages secrete an enzyme which hydrolyzes [3H]elastin prepared by NaB3H4 reduction of bovine ligamentum nuchae elastin. Tritium 98-100 elastin Bos taurus 166-173 1313693-4 1992 Specific nuclear translocation of [3H]calcitriol/VDR was found to be time dependent reaching a maximum of approximately 2100 binding sites/nucleus after 3 h of incubation in HL60 cells, whereas a maximum of approximately 310 binding sites/nucleus was found after 3 h in PBL. Tritium 35-37 vitamin D receptor Homo sapiens 49-52 6901615-3 1980 10(6) thioglycollate-elicited cells secrete sufficient enzyme to solubilize 200--350 micrograms [3H]elastin in 18h. Tritium 97-99 elastin Mus musculus 100-107 6901615-6 1980 Enzyme activity is linearly related to the amount of conditioned medium assayed and is linear over incubation times up to 30 h. Unlabelled elastin competitively inhibits the solubilization of [3H]elastin. Tritium 193-195 elastin Mus musculus 139-146 6901615-6 1980 Enzyme activity is linearly related to the amount of conditioned medium assayed and is linear over incubation times up to 30 h. Unlabelled elastin competitively inhibits the solubilization of [3H]elastin. Tritium 193-195 elastin Mus musculus 196-203 7390396-1 1980 Somatomedin activity as determined by the stimulation of [3H]thymidine incorporation into chick embryo fibroblast DNA, was measured in extracts of sera from genetically small mice. Tritium 58-60 insulin like growth factor 1 Gallus gallus 0-11 34736959-4 2022 Autoradiography of (3H) 69,593 binding to KOR in brain sections showed that WT and K4A mice had similar KOR distribution and expression levels in brain regions without sex differences. Tritium 20-22 opioid receptor, kappa 1 Mus musculus 42-45 7393204-0 1980 Electrophoretic analysis of 3H-pargyline-labeled monoamine oxidases A and B from human and rat cells. Tritium 28-30 monoamine oxidase A Homo sapiens 49-75 34599072-7 2021 Out of the ten OSTalpha/beta mutants investigated, four (S228K, T229S, Q269E, Q269K) exhibited decreased (3H)-taurocholate (TCA) uptake (ratio of geometric means relative to OSTalpha/beta WT of 0.76, 0.75, 0.79, 0.13, respectively). Tritium 106-108 solute carrier family 51 subunit alpha Homo sapiens 15-28 34599072-8 2021 Three OSTalpha/beta mutants (S228K, Q269K, E305A) had reduced (3H)-TCA efflux % (ratio of geometric means relative to OSTalpha/beta WT of 0.86, 0.65, 0.79, respectively). Tritium 63-65 solute carrier family 51 subunit alpha Homo sapiens 6-19 500638-2 1979 Saturable, high affinity binding of tritium-labeled saxitoxin ([3H]STX) to axolemma-enriched membranes from white matter of bovine brain was identified. Tritium 36-43 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 Homo sapiens 67-70 35622458-9 2022 The combination of T&S-CD9 risk level and TNM stage were accurate predictors of OS (C-index: 0.676; AIC: 512.51) and RFS (C-index: 0.680; AIC: 519.53). Tritium 19-22 CD9 molecule Homo sapiens 23-26 500638-2 1979 Saturable, high affinity binding of tritium-labeled saxitoxin ([3H]STX) to axolemma-enriched membranes from white matter of bovine brain was identified. Tritium 64-66 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 Homo sapiens 67-70 500638-3 1979 The apparent [3H]STX equilibrium dissociation constant (Kd*) was strongly affected by the cationic environment:choline ion had little effect; cesium ion increased the mammalian axolemma Kd* in a simple competitive manner. Tritium 14-16 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 Homo sapiens 17-20 516072-2 1979 In the meantime liver weight and water content underwent changes, glycogen was lost, triglycerides accumulated in the liver while decreasing in serum, [3H] leucine uptake in proteins was impaired and the activity of glucose-6-phosphatase and aminopyrine demethylase decreased. Tritium 152-154 glucose-6-phosphatase catalytic subunit 1 Rattus norvegicus 216-237 34983574-10 2022 An increase in uptake of (3H)-glucose was demonstrated, while a non-significant increase in the efflux ratio of the P-gp substrate (3H)-digoxin was observed in hypoxic cells. Tritium 132-134 phosphoglycolate phosphatase Bos taurus 116-120 35372502-8 2022 SLC6A14 inhibition with a specific pharmacological blocker reduced 3H-arginine transport, mTOR phosphorylation, and bronchial epithelial repair rates in wound healing assays. Tritium 67-69 solute carrier family 6 member 14 Homo sapiens 0-7 2696384-2 1989 As demonstrated for [3H]palmitoylated RAS1 protein of Saccharomyces cerevisiae and the acylated acyl carrier protein of Spirodela oligorrhiza, this procedure alleviates the need for elution of proteins from polyacrylamide gel slices. Tritium 21-23 Ras family GTPase RAS1 Saccharomyces cerevisiae S288C 38-42 37081-4 1979 The four histidine C-2 proton resonances have been assigned to specific residues in the amino-acid sequence, by means of deuterium and tritium exchange experiments on the alpha subunit and its des(92-96) derivative. Tritium 135-142 complement C2 Homo sapiens 19-22 2697960-4 1989 MGF-stimulated MO synthesized DNA, as measured by cellular incorporation of tritium-labeled thymidine (3H-TdR). Tritium 76-83 KIT ligand Sus scrofa 0-3 2697960-4 1989 MGF-stimulated MO synthesized DNA, as measured by cellular incorporation of tritium-labeled thymidine (3H-TdR). Tritium 103-105 KIT ligand Sus scrofa 0-3 2530275-3 1989 MGF, pTGF-beta 1, and pTGF-beta 2 decreased mitogen-induced [3H]thymidine incorporation by 30 to 75% in a dose-dependent manner. Tritium 61-63 growth differentiation factor 15 Homo sapiens 5-14 2530275-3 1989 MGF, pTGF-beta 1, and pTGF-beta 2 decreased mitogen-induced [3H]thymidine incorporation by 30 to 75% in a dose-dependent manner. Tritium 61-63 growth differentiation factor 15 Homo sapiens 22-31 221899-1 1979 Covalently bound [3H]D-Ala2,Met5-enkephalinamide- and 125I-labeled D-Ala2,N-Me-Phe4,Met-(O)5-ol-enkephalin-macromolecule complexes have been prepared by crosslinking the solubilized noncovalent complexes from rat brain. Tritium 18-20 proenkephalin Rattus norvegicus 33-43 2530275-10 1989 Furthermore, MGF, pTGF-beta 1, and pTGF-beta 2 also decreased IL-2 mediated [3H]thymidine incorporation into human PBL Con A blasts and the IL-4-mediated [3H]thymidine incorporation of purified T lymphocytes costimulated with PDBu by 70%. Tritium 77-79 growth differentiation factor 15 Homo sapiens 18-27 2530275-10 1989 Furthermore, MGF, pTGF-beta 1, and pTGF-beta 2 also decreased IL-2 mediated [3H]thymidine incorporation into human PBL Con A blasts and the IL-4-mediated [3H]thymidine incorporation of purified T lymphocytes costimulated with PDBu by 70%. Tritium 77-79 growth differentiation factor 15 Homo sapiens 35-44 2794143-8 1989 Our studies show that a postnatal decrease in the density of [3H]-HC3 sites in the patch/striosomes and an increase in the matrix density of [3H]-MAZ sites are the primary, but not the sole, changes in the compartmental distribution of these sites leading to the adult striosomal organization of the striatal cholinergic and dopaminergic innervation. Tritium 142-144 LOW QUALITY PROTEIN: myc-associated zinc finger protein Papio anubis 146-149 474274-8 1979 Both 3H-CI-628 and 3H-U-23, 469 are metabolized in vivo to more polar forms which are found associated with the nuclear estrogen receptor, and which may be the true agents active in vivo. Tritium 5-7 estrogen receptor 1 Rattus norvegicus 120-137 2794143-9 1989 D2 receptors follow the general developmental pattern of [3H]-MAZ and [3H]-HC3, changing their density of distribution in both compartments during the developmental period examined. Tritium 58-60 LOW QUALITY PROTEIN: myc-associated zinc finger protein Papio anubis 62-65 474274-8 1979 Both 3H-CI-628 and 3H-U-23, 469 are metabolized in vivo to more polar forms which are found associated with the nuclear estrogen receptor, and which may be the true agents active in vivo. Tritium 19-21 estrogen receptor 1 Rattus norvegicus 120-137 2509230-5 1989 The increase with IRBP concentration accompanied, and presumably resulted from, an increased transfer of [3H]retinol to the RPE-eyecup. Tritium 106-108 retinol binding protein 3 Bos taurus 18-22 34404-0 1979 A new assay for glutathione S-transferase using [3H]-benzo(a)pyrene 4,5-oxide as substrate. Tritium 49-51 hematopoietic prostaglandin D synthase Rattus norvegicus 16-41 2476477-4 1989 In vitro studies showed that when quiescent (Ki-67-negative) NIH 3T3 fibroblasts or bovine peripheral blood lymphocytes were induced to proliferate, the appearance of Ki-67-positive cells paralleled the induction of cell proliferation caused by addition of fetal calf serum or PHA, respectively, to the cultures, and in both human and rat proliferating cells the Ki-67 expression closely paralleled the incorporation of [3H]-thymidine. Tritium 421-423 antigen identified by monoclonal antibody Ki 67 Mus musculus 167-172 2476477-4 1989 In vitro studies showed that when quiescent (Ki-67-negative) NIH 3T3 fibroblasts or bovine peripheral blood lymphocytes were induced to proliferate, the appearance of Ki-67-positive cells paralleled the induction of cell proliferation caused by addition of fetal calf serum or PHA, respectively, to the cultures, and in both human and rat proliferating cells the Ki-67 expression closely paralleled the incorporation of [3H]-thymidine. Tritium 421-423 antigen identified by monoclonal antibody Ki 67 Mus musculus 167-172 369847-4 1978 The ratio of [14C]A-LH to [3H]GA-LH (14C:3H ratio) in the tissue decreased significantly with time and with GnRH treatment. Tritium 27-29 gonadotropin releasing hormone 1 Rattus norvegicus 108-112 369847-4 1978 The ratio of [14C]A-LH to [3H]GA-LH (14C:3H ratio) in the tissue decreased significantly with time and with GnRH treatment. Tritium 41-43 gonadotropin releasing hormone 1 Rattus norvegicus 108-112 2792733-3 1989 Our data revealed significant seasonal variations in both receptor content and the ER"s affinity for [3H]E2 in the liver of the turtle. Tritium 102-104 estrogen receptor Chrysemys picta 83-85 369847-5 1978 In the second experiment, replenishment of GnRH in the medium every 0.5 h elevated the release rate (release during each sequential 0.5 h) of both [3H]GA and [14C]A-labeled LH within 1.5 h. The release rate of radiolabeled LH increased linearly until 3.5 h. The 14C:3H ratio in LH released during each time interval was reduced by GnRH. Tritium 148-150 gonadotropin releasing hormone 1 Rattus norvegicus 43-47 369847-5 1978 In the second experiment, replenishment of GnRH in the medium every 0.5 h elevated the release rate (release during each sequential 0.5 h) of both [3H]GA and [14C]A-labeled LH within 1.5 h. The release rate of radiolabeled LH increased linearly until 3.5 h. The 14C:3H ratio in LH released during each time interval was reduced by GnRH. Tritium 266-268 gonadotropin releasing hormone 1 Rattus norvegicus 43-47 369847-7 1978 In other experiments, cycloheximide blocked synthesis of [14C]A-LH and greatly reduced the GnRH-induced synthesis and release of [3H]GA-LH, but reduced release of IR-LH by only 25%. Tritium 130-132 gonadotropin releasing hormone 1 Rattus norvegicus 91-95 2548952-4 1989 Both priming and triggering agents inhibited [3H]-thymidine uptake by macrophages stimulated with CSF-1. Tritium 46-48 colony stimulating factor 1 (macrophage) Mus musculus 98-103 19605227-0 1978 Specific [(3)H]corticosterone uptake in the hippocampus and septum varies with social settings in mice: Hormone-receptor autoregulation may be involved. Tritium 10-14 nuclear receptor subfamily 4, group A, member 1 Mus musculus 104-120 2542463-6 1989 When these compounds were examined for their ability to inhibit [3H]NAAG binding to membranes of rat forebrain, the relative order of potency was L-alpha-aminoadipate = D-alpha-aminoadipate greater than L-AP4 greater than L-serine-O-phosphate greater than D-AP4 much greater than D,L-AP3. Tritium 65-67 replication initiator 1 Rattus norvegicus 205-208 204498-3 1978 3H-Methionine enkephalin associates and dissociates from the opiate receptor with 8--10 fold slower kinetics than 3H-opiates. Tritium 0-2 proenkephalin Rattus norvegicus 14-24 2577255-1 1989 We studied blocking type TSH receptor antibodies in 28 patients with primary myxedema and 21 patients with goitrous Hashimoto"s thyroiditis by measuring the ability of their IgG to inhibit TSH binding to its receptor, and to inhibit TSH-stimulated cAMP increases and 3H-thymidine incorporation in a rat thyroid cell line, FRTL-5. Tritium 267-269 thyroid stimulating hormone receptor Homo sapiens 25-37 2739687-2 1989 DNA binding of 3H-IQ was catalyzed by both microsomes and S9 and was increased 5-fold in Aroclor (PCB)-pretreated animals. Tritium 15-17 pyruvate carboxylase Rattus norvegicus 98-101 2767136-3 1989 Specific [3H]NMS binding to whole cell preparations of SH-EP 1 could not be detected. Tritium 10-12 SH2 domain containing 3C Homo sapiens 55-62 632230-9 1978 i) EF-1betagamma catalytically promoted the exchange of [14C]GDP bound to EF-1alpha with exogenous [3H]GDP. Tritium 100-102 EF1ALPHA Sus scrofa 74-83 2542761-7 1989 The effects of MCDF on the uptake and persistence of nuclear [3H]TCDD-Ah receptor complex levels were also determined in rat liver and rat hepatoma H-4-II E cells in culture. Tritium 62-64 aryl hydrocarbon receptor Rattus norvegicus 70-81 2544927-5 1989 Bones labeled with [3H]-AA and incubated with transforming growth factor-alpha (TGF-alpha), which stimulates endogenous PGE production, produced substantial amounts of PGE2, while bones labeled with [3H]-EPA and treated similarly produced less than 1/10th as much labeled PGE3. Tritium 20-22 transforming growth factor alpha Rattus norvegicus 80-89 728204-0 1978 A tritium-release in vivo assay of dopamine-beta-hydroxylase activity in sympathetic neurons. Tritium 2-9 dopamine beta-hydroxylase Homo sapiens 35-60 2545464-3 1989 The binding of [3H]PAF was saturable, specific, time-dependent and reversible. Tritium 16-18 PCNA clamp associated factor Rattus norvegicus 19-22 304785-3 1977 In studies in vitro with T lymphocytes, D-PA at 1 mM concentration inhibited both concanavalin A- and phytohaemagglutinin-induced transformation as assayed by [3H]thymidine incorporation, but D-PA concentrations of 5 mM were required to inhibit concanavalin A-induced amino acid uptake. Tritium 160-162 D-phenylalanine Mus musculus 40-44 2665758-6 1989 Competitive studies indicated that TRK-100 was 1.5 times less active than prostacyclin (epoprostenol, PGI2), but was 3 times more potent than PGE1 in displacing [3H]-TRK-100 from the binding sites on rat platelets. Tritium 162-164 neurotrophic receptor tyrosine kinase 1 Rattus norvegicus 35-38 2665758-6 1989 Competitive studies indicated that TRK-100 was 1.5 times less active than prostacyclin (epoprostenol, PGI2), but was 3 times more potent than PGE1 in displacing [3H]-TRK-100 from the binding sites on rat platelets. Tritium 162-164 neurotrophic receptor tyrosine kinase 1 Rattus norvegicus 166-169 321219-6 1977 There was a significant positive interaction between EB and GnRH on the amounts of [3H]glucosamine-LH and [14C]alanine-LH in the medium and of [3H]glucosamine-LH in the tissue and total system. Tritium 84-87 gonadotropin releasing hormone 1 Rattus norvegicus 60-64 138443-12 1977 There are a number of [3H]ouabain binding sites which remain resistant to lipolysis by phospholipase A in either untreated or detergent-treated membrane preparations. Tritium 23-25 phospholipase A and acyltransferase 1 Homo sapiens 87-102 2539818-5 1989 AAG-treated rats had greater [3H]propranolol binding in serum (93.0 +/- 3.2 vs 76.7 +/- 3.0%, P less than 0.01) and a lower calculated unbound [3H]propranolol concentration in serum (2.7 +/- 1.3 vs 7.4 +/- 3.1 X 10(6) dpm/ml, P less than 0.001) than albumin-treated rats. Tritium 30-32 orosomucoid 1 Rattus norvegicus 0-3 2539818-5 1989 AAG-treated rats had greater [3H]propranolol binding in serum (93.0 +/- 3.2 vs 76.7 +/- 3.0%, P less than 0.01) and a lower calculated unbound [3H]propranolol concentration in serum (2.7 +/- 1.3 vs 7.4 +/- 3.1 X 10(6) dpm/ml, P less than 0.001) than albumin-treated rats. Tritium 144-146 orosomucoid 1 Rattus norvegicus 0-3 832136-2 1977 Quaking mice and littermate controls received intracranial injections of 150 muCi [3H]glycine and 25 muCi of [14C]glycine respectively. Tritium 83-85 quaking, KH domain containing RNA binding Mus musculus 0-7 2538564-0 1989 Characterization and regional distribution of alpha 2-adrenoceptors in postmortem human brain using the full agonist [3H]UK 14304. Tritium 118-120 adrenoceptor alpha 2A Homo sapiens 46-67 2767286-10 1989 3H-oxytocin degradation was studied in vitro in pooled plasma of pregnant and nonpregnant women. Tritium 0-2 oxytocin/neurophysin I prepropeptide Homo sapiens 3-11 868650-4 1977 The precursor relationship of soluble to insoluble elastin was demonstrated by incubating the [3H]-labeled soluble elastin from copper-deficient pig aorta with the culture and isolating labeled crosslinks from the insoluble elastin residue. Tritium 95-97 LOC100620140 Sus scrofa 51-58 2767286-11 1989 85% of original 3H-oxytocin was destroyed in pregnant plasma during 1 hour but only below 10% was destroyed in nonpregnant plasma in 2 hours. Tritium 16-18 oxytocin/neurophysin I prepropeptide Homo sapiens 19-27 868650-4 1977 The precursor relationship of soluble to insoluble elastin was demonstrated by incubating the [3H]-labeled soluble elastin from copper-deficient pig aorta with the culture and isolating labeled crosslinks from the insoluble elastin residue. Tritium 95-97 LOC100620140 Sus scrofa 115-122 2706318-1 1989 The distribution, metabolism, and pharmacokinetics of physostigmine (Phy) and the time course of butyrylcholinesterase (BuChE) in plasma and cholinesterase (ChE) activity in brain and muscle and their relationship to Phy concentration were described after oral administration of 3H-Phy (650 micrograms kg-1) to rats. Tritium 279-281 butyrylcholinesterase Rattus norvegicus 120-125 868650-4 1977 The precursor relationship of soluble to insoluble elastin was demonstrated by incubating the [3H]-labeled soluble elastin from copper-deficient pig aorta with the culture and isolating labeled crosslinks from the insoluble elastin residue. Tritium 95-97 LOC100620140 Sus scrofa 115-122 15625864-5 1976 In the presence of 100 mM NaCl, binding of [3H] met-enkephalin was 90-95% reduced, thus indicating the agonist properties of the peptide. Tritium 44-46 proenkephalin Rattus norvegicus 52-62 2777919-5 1989 In contrast, gp-3 and gp-2 incorporated [3H]galactose and [3H]glucosamine but not [3H]mannose. Tritium 41-43 glycoprotein 2 (zymogen granule membrane) Mus musculus 22-26 2777919-5 1989 In contrast, gp-3 and gp-2 incorporated [3H]galactose and [3H]glucosamine but not [3H]mannose. Tritium 59-61 glycoprotein 2 (zymogen granule membrane) Mus musculus 22-26 10754-0 1976 A sensitive and specific tritium release assay for dopamine-beta-hydroxylase (DbetaH) in serum. Tritium 25-32 dopamine beta-hydroxylase Homo sapiens 51-76 2777919-5 1989 In contrast, gp-3 and gp-2 incorporated [3H]galactose and [3H]glucosamine but not [3H]mannose. Tritium 59-61 glycoprotein 2 (zymogen granule membrane) Mus musculus 22-26 182361-9 1976 The binding of [3H]BP to lung nuclei was greatly enhanced by liver microsomes but only slightly by lung microsomes, which had rather low AHH activity. Tritium 16-18 cytochrome P450, family 1, subfamily a, polypeptide 1 Rattus norvegicus 137-140 2465355-2 1989 We have used primary mouse keratinocytes as a model system to compare the uptake and metabolism of [3H] retinol delivered to them either free in solution or bound to RBP. Tritium 100-102 retinol binding protein 4, plasma Mus musculus 166-169 2465355-3 1989 RBP was purified from rat serum, loaded with [3H]retinol, and the [3H]retinol-RBP complex purified by affinity chromatography on human transthyretin-Sepharose. Tritium 46-48 retinol binding protein 4 Homo sapiens 0-3 2465355-3 1989 RBP was purified from rat serum, loaded with [3H]retinol, and the [3H]retinol-RBP complex purified by affinity chromatography on human transthyretin-Sepharose. Tritium 67-69 retinol binding protein 4 Homo sapiens 0-3 2465355-3 1989 RBP was purified from rat serum, loaded with [3H]retinol, and the [3H]retinol-RBP complex purified by affinity chromatography on human transthyretin-Sepharose. Tritium 67-69 retinol binding protein 4 Homo sapiens 78-81 2465355-4 1989 Keratinocytes incubated with either free [3H]retinol or [3H]retinol-RBP complex accumulated [3H]retinol in a time and temperature dependent manner. Tritium 57-59 retinol binding protein 4, plasma Mus musculus 68-71 2465355-4 1989 Keratinocytes incubated with either free [3H]retinol or [3H]retinol-RBP complex accumulated [3H]retinol in a time and temperature dependent manner. Tritium 57-59 retinol binding protein 4, plasma Mus musculus 68-71 2725816-4 1989 DPPC-induced reductions in [3H]ACh levels were blocked by 100 microM eserine, a tertiary amine cholinesterase inhibitor, but not with 100 microM neostigmine, a quaternary ammonium inhibitor. Tritium 28-30 butyrylcholinesterase Rattus norvegicus 95-109 2562956-8 1989 [3H]Azidopine labeling of P-GP from these two sources was inhibited by various steroids, particularly progesterone. Tritium 1-3 phosphoglycolate phosphatase Mus musculus 26-30 954850-1 1976 In slices of rat occipital cortex preincubated with 3H-noradrenaline, methioine-enkephalin diminished the overflow of tritium evoked by electrical field stimulation or by 20 mM potassium. Tritium 118-125 proenkephalin Rattus norvegicus 80-90 2818265-2 1989 Transferrin stimulated the mitotic and [3H]-thymidine labelling indices. Tritium 40-42 transferrin Mus musculus 0-11 1100120-5 1975 However, the peak of IB2 resolved on the gel showed an increased 3H/14C ratio in comparison to the majority of the other cellular proteins. Tritium 65-67 mitogen-activated protein kinase 8 interacting protein 2 Homo sapiens 21-24 2783247-7 1989 Furthermore, simultaneous exposure of BMMC for 4 h to 100 ng/ml of rGM-CSF reduced the mean DNA incorporation of [3H]AZT (10 microM) from 27.2 to 19.1 pmol/micrograms of DNA. Tritium 114-116 colony stimulating factor 2 Rattus norvegicus 67-74 2491757-6 1989 Activation of PKC resulted in a facilitated transmitter release in tissue from 6- and 12-month-old animals but reduced [3H]5-HT release in slices from 24-month-old animals. Tritium 120-122 protein kinase C, gamma Rattus norvegicus 14-17 1092790-5 1975 The possibility is discussed that the F1 responding T cells belong to a subpopulation performing a suppressive function; MLC lacking F1 T cells showed increased [3H] thymidine incorporation. Tritium 162-164 megalencephalic leukoencephalopathy with subcortical cysts 1 homolog (human) Mus musculus 121-124 2760962-6 1989 The concentration of cellular 3H-retinol was more than 10 fold greater in germinal cells administered 3H-retinol bound to bovine serum albumin than when 3H-retinol bound to serum retinol-binding protein was administered, and likewise the concentration of cellular 3H-retinyl palmitate was approximately 30 fold higher. Tritium 30-32 albumin Rattus norvegicus 129-142 1138993-3 1975 Enzymic oxidation of UDP-glucose-6-3-H with calf liver UDP-glucose dehydrogenase was found to proceed with direct transfer of the hydrogen from C-6 of UDP-glucose onto NAD. Tritium 34-38 UDP-glucose 6-dehydrogenase Bos taurus 55-80 2547173-2 1989 Previous research determined that exogenous 5-HT and 5-HT1B agonists, in adult (3 months) rats, activated the 5-HT presynaptic receptor resulting in a dose-dependent decrease in 3H-5-HT release from spinal cord slices. Tritium 178-180 5-hydroxytryptamine receptor 1B Rattus norvegicus 53-59 2547173-3 1989 Contrastively, exogenous 5-HT and the selective 5-HT1B agonist 1-(m-chlorophenyl) piperazine HCl (mCPP) produced a dose-dependent increase in depolarization-evoked 3H-5-HT release from spinal cord of aged rats. Tritium 164-166 5-hydroxytryptamine receptor 1B Rattus norvegicus 48-54 2325406-0 1990 The binding of 3H-labelled androgen-receptor complexes to hypothalamic chromatin of neonatal mice: effect of sex and androgenization. Tritium 15-17 androgen receptor Mus musculus 27-44 2325406-1 1990 The binding of 3H-labelled androgen-receptor complexes, prepared by (NH4)2SO4 precipitation from the 105,000 g supernatant of hypothalamic cytosol, to hypothalamic chromatin of neonatal mice covalently coupled to cellulose was measured in vitro. Tritium 15-17 androgen receptor Mus musculus 27-44 2340856-7 1990 Yet, regions enriched in 5-HT1A and 5-HT1C sites (e.g. dentate gyrus and choroid plexus, respectively) revealed relatively more [3H]5-HT binding as compared to [3H]eltoprazine binding, whereas [3H]eltoprazine binding was more pronounced in 5-HT1B receptor dense areas such as the dorsal subiculum, substantia nigra, ventral pallidum and globus pallidus. Tritium 129-131 5-hydroxytryptamine receptor 2C Rattus norvegicus 36-42 2340856-7 1990 Yet, regions enriched in 5-HT1A and 5-HT1C sites (e.g. dentate gyrus and choroid plexus, respectively) revealed relatively more [3H]5-HT binding as compared to [3H]eltoprazine binding, whereas [3H]eltoprazine binding was more pronounced in 5-HT1B receptor dense areas such as the dorsal subiculum, substantia nigra, ventral pallidum and globus pallidus. Tritium 161-163 5-hydroxytryptamine receptor 2C Rattus norvegicus 36-42 2340856-7 1990 Yet, regions enriched in 5-HT1A and 5-HT1C sites (e.g. dentate gyrus and choroid plexus, respectively) revealed relatively more [3H]5-HT binding as compared to [3H]eltoprazine binding, whereas [3H]eltoprazine binding was more pronounced in 5-HT1B receptor dense areas such as the dorsal subiculum, substantia nigra, ventral pallidum and globus pallidus. Tritium 161-163 5-hydroxytryptamine receptor 2C Rattus norvegicus 36-42 2466220-0 1989 Pharmacological and mechanistic studies of cholecystokinin-facilitated [3H]dopamine efflux from rat nucleus accumbens. Tritium 72-74 cholecystokinin Rattus norvegicus 43-58 127883-5 1975 The incorporation of tritium into the peptide N-H groups of myosin was also decreased during muscle activity. Tritium 21-28 myosin heavy chain 14 Homo sapiens 60-66 2466220-2 1989 In an attempt to characterize the nature of CCK interactions with mesolimbic DA-containing nerve terminals, we have investigated CCK regulation of [3H]DA overflow from rat nucleus accumbens slices. Tritium 148-150 cholecystokinin Rattus norvegicus 129-132 2466220-3 1989 CCK-8 produced a saturable and potent (EC50 = 3 nM) facilitation of KCl (35 mM)-evoked [3H]DA efflux from nucleus accumbens, but failed to significantly alter [3H]DA efflux from striatum: The stimulatory action of CCK-8 was unaffected by the muscarinic antagonist atropine, the opiate antagonist naloxone, or the selective ion channel blockers tetrodotoxin and nifedipine. Tritium 88-90 cholecystokinin Rattus norvegicus 0-3 2469026-8 1989 MBP was not labeled with [3H]myristate, but was slightly labeled with both 14C-essential fatty acids. Tritium 26-28 myelin basic protein Oryctolagus cuniculus 0-3 2645590-4 1989 Human skin extracts incubated with either [3H]retinol or [3H]retinoic acid and analyzed by PAGE is a novel technique for the study of cellular retinol-(CRBP) and retinoic acid-(CRABP) binding proteins; it allows one to more specifically analyse these binding proteins and differentiate them from RBP. Tritium 43-45 retinol binding protein 4 Homo sapiens 153-156 2154953-3 1990 The yield of [3H]PAF could be dramatically increased by pretreating the cells with either oleic acid, an activator of CTP:phosphocholine cytidylyltransferase, or phenylmethylsulfonyl fluoride, an inhibitor of PAF acetylhydrolase. Tritium 14-16 phospholipase A2 group VII Homo sapiens 209-228 2297707-2 1990 Initial studies indicated that in vitro [3H]MPCU was concentrated 4- to 6-fold in GC3/c1 human colon adenocarcinoma cells in an azide-sensitive manner. Tritium 41-43 ankyrin repeat domain 36 Mus musculus 82-85 2227125-1 1990 Insulin was found to provoke rapid increases in diacylglycerol (DAG) content and [3H]glycerol incorporation into DAG and other lipids during incubations of rat hemidiaphragms and soleus muscles. Tritium 82-84 insulin Canis lupus familiaris 0-7 2227125-5 1990 The importance of insulin-induced increases in DAG-PKC signaling in the stimulation of glucose transport in rat diaphragm and soleus muscles was suggested by 1) PKC activators phorbol esters and phospholipase C stimulation of [3H]-2-deoxyglucose (DOG) uptake and 2) PKC inhibitors staurosporine and polymixin B inhibition of insulin effects on [3H]-2-DOG uptake. Tritium 227-229 insulin Canis lupus familiaris 18-25 2227125-5 1990 The importance of insulin-induced increases in DAG-PKC signaling in the stimulation of glucose transport in rat diaphragm and soleus muscles was suggested by 1) PKC activators phorbol esters and phospholipase C stimulation of [3H]-2-deoxyglucose (DOG) uptake and 2) PKC inhibitors staurosporine and polymixin B inhibition of insulin effects on [3H]-2-DOG uptake. Tritium 345-347 insulin Canis lupus familiaris 18-25 2308260-2 1990 PAF content was assessed following extraction, isolation and quantification of this alkyl ether lipid using a bioassay based on [3H]-serotonin release from labelled rabbit platelets. Tritium 129-131 PCNA clamp associated factor Rattus norvegicus 0-3 2512639-3 1989 The enzyme activity of live cells was measured by soluble [3H]elastin hydrolysis first under basic conditions and then after immune complex stimulation. Tritium 59-61 elastin Homo sapiens 62-69 2641410-1 1989 Mucin biosynthesis in the rat gastrointestinal tract was measured by following the incorporation of radioactive precursors, [3H] glucosamine, [3H] galactose and [3H] serine. Tritium 124-128 solute carrier family 13 member 2 Rattus norvegicus 0-5 2641410-1 1989 Mucin biosynthesis in the rat gastrointestinal tract was measured by following the incorporation of radioactive precursors, [3H] glucosamine, [3H] galactose and [3H] serine. Tritium 125-127 solute carrier family 13 member 2 Rattus norvegicus 0-5 2641410-1 1989 Mucin biosynthesis in the rat gastrointestinal tract was measured by following the incorporation of radioactive precursors, [3H] glucosamine, [3H] galactose and [3H] serine. Tritium 143-145 solute carrier family 13 member 2 Rattus norvegicus 0-5 5420953-12 1970 Injection of testosterone propionate into animals castrated 7 days previously induced a rapid increase in both enzymic activities: ornithine decarboxylase was doubled in 6h, and increased three- to four-fold within 48h, whereas the putrescine-dependent decarboxylation of S-adenosyl-l-methionine doubled in 3h and increased tenfold within 48h of commencement of daily androgen treatments. Tritium 307-309 ornithine decarboxylase 1 Rattus norvegicus 131-154 2849666-5 1988 In competition studies for the displacement of [3H]LTB4 from binding sites, LTB4 and its analogs had the following order of potency: LTB4 (Ki = 2.9 +/- 0.8 nM) greater than 5-epi LTB4 (Ki = 58.7 +/- 0.3 nM) greater than 5-deoxy-LTB4 (Ki = 91.7 +/- 0.3 nM) greater than 12-epi LTB4 (Ki = 4.7 +/- 1.2 microM) greater than 5,12-deoxy LTB4 (Ki = 7.6 +/- 0.01 microM) greater than or equal to 12-deoxy LTB4 (Ki = 8.9 +/- 0.01 microM). Tritium 48-50 prostaglandin reductase 1 Cavia porcellus 76-80 2849666-1 1988 Specific binding sites for [3H]leukotriene B4 (LTB4) were identified on guinea pig lung macrophages, using high specific activity [3H] LTB4 in the presence or absence of unlabeled LTB4. Tritium 28-30 prostaglandin reductase 1 Cavia porcellus 47-51 2849666-1 1988 Specific binding sites for [3H]leukotriene B4 (LTB4) were identified on guinea pig lung macrophages, using high specific activity [3H] LTB4 in the presence or absence of unlabeled LTB4. Tritium 28-30 prostaglandin reductase 1 Cavia porcellus 135-139 2849666-1 1988 Specific binding sites for [3H]leukotriene B4 (LTB4) were identified on guinea pig lung macrophages, using high specific activity [3H] LTB4 in the presence or absence of unlabeled LTB4. Tritium 28-30 prostaglandin reductase 1 Cavia porcellus 135-139 2849666-2 1988 At 0 degrees C, [3H] LTB4 binding reached equilibrium within 30 min, and addition of a large excess of unlabeled LTB4 resulted in a rapid decrease of specific binding. Tritium 17-19 prostaglandin reductase 1 Cavia porcellus 21-25 2849666-5 1988 In competition studies for the displacement of [3H]LTB4 from binding sites, LTB4 and its analogs had the following order of potency: LTB4 (Ki = 2.9 +/- 0.8 nM) greater than 5-epi LTB4 (Ki = 58.7 +/- 0.3 nM) greater than 5-deoxy-LTB4 (Ki = 91.7 +/- 0.3 nM) greater than 12-epi LTB4 (Ki = 4.7 +/- 1.2 microM) greater than 5,12-deoxy LTB4 (Ki = 7.6 +/- 0.01 microM) greater than or equal to 12-deoxy LTB4 (Ki = 8.9 +/- 0.01 microM). Tritium 48-50 prostaglandin reductase 1 Cavia porcellus 51-55 2849666-5 1988 In competition studies for the displacement of [3H]LTB4 from binding sites, LTB4 and its analogs had the following order of potency: LTB4 (Ki = 2.9 +/- 0.8 nM) greater than 5-epi LTB4 (Ki = 58.7 +/- 0.3 nM) greater than 5-deoxy-LTB4 (Ki = 91.7 +/- 0.3 nM) greater than 12-epi LTB4 (Ki = 4.7 +/- 1.2 microM) greater than 5,12-deoxy LTB4 (Ki = 7.6 +/- 0.01 microM) greater than or equal to 12-deoxy LTB4 (Ki = 8.9 +/- 0.01 microM). Tritium 48-50 prostaglandin reductase 1 Cavia porcellus 76-80 2407274-6 1990 Acidic fibroblast growth factor (FGFa) stimulated [3H]thymidine incorporation by 16-fold with half-maximal stimulation at 20 ng/ml, whereas basic FGF (FGFb) only stimulated type II cell DNA synthesis by 3-fold. Tritium 51-53 fibroblast growth factor 1 Rattus norvegicus 0-31 2172674-3 1990 In vitro expression of this gene in murine fibroblasts (B82) via the eukaryotic expression vector, pH beta APr-1-neo, resulted in high level expression of specific [3H] (-)MQNB binding in transfected B82 cell lines. Tritium 165-167 MAGE family member H1 Mus musculus 107-112 2174491-1 1990 Pharmacological characterization of the Nb2 cell peripheral-type benzodiazepine receptor (PBR) was determined using selected 1,4-benzodiazepines, PK 11195, and protoporphyrin IX (PPIX) to compete for specific [3H] Ro5-4864 binding. Tritium 210-212 translocator protein Rattus norvegicus 49-88 2174491-1 1990 Pharmacological characterization of the Nb2 cell peripheral-type benzodiazepine receptor (PBR) was determined using selected 1,4-benzodiazepines, PK 11195, and protoporphyrin IX (PPIX) to compete for specific [3H] Ro5-4864 binding. Tritium 210-212 translocator protein Rattus norvegicus 90-93 2849666-5 1988 In competition studies for the displacement of [3H]LTB4 from binding sites, LTB4 and its analogs had the following order of potency: LTB4 (Ki = 2.9 +/- 0.8 nM) greater than 5-epi LTB4 (Ki = 58.7 +/- 0.3 nM) greater than 5-deoxy-LTB4 (Ki = 91.7 +/- 0.3 nM) greater than 12-epi LTB4 (Ki = 4.7 +/- 1.2 microM) greater than 5,12-deoxy LTB4 (Ki = 7.6 +/- 0.01 microM) greater than or equal to 12-deoxy LTB4 (Ki = 8.9 +/- 0.01 microM). Tritium 48-50 prostaglandin reductase 1 Cavia porcellus 76-80 2849666-5 1988 In competition studies for the displacement of [3H]LTB4 from binding sites, LTB4 and its analogs had the following order of potency: LTB4 (Ki = 2.9 +/- 0.8 nM) greater than 5-epi LTB4 (Ki = 58.7 +/- 0.3 nM) greater than 5-deoxy-LTB4 (Ki = 91.7 +/- 0.3 nM) greater than 12-epi LTB4 (Ki = 4.7 +/- 1.2 microM) greater than 5,12-deoxy LTB4 (Ki = 7.6 +/- 0.01 microM) greater than or equal to 12-deoxy LTB4 (Ki = 8.9 +/- 0.01 microM). Tritium 48-50 prostaglandin reductase 1 Cavia porcellus 76-80 5347612-2 1969 Identification of 3H-labeled HPL. Tritium 18-20 galectin 1 Homo sapiens 29-32 2849666-5 1988 In competition studies for the displacement of [3H]LTB4 from binding sites, LTB4 and its analogs had the following order of potency: LTB4 (Ki = 2.9 +/- 0.8 nM) greater than 5-epi LTB4 (Ki = 58.7 +/- 0.3 nM) greater than 5-deoxy-LTB4 (Ki = 91.7 +/- 0.3 nM) greater than 12-epi LTB4 (Ki = 4.7 +/- 1.2 microM) greater than 5,12-deoxy LTB4 (Ki = 7.6 +/- 0.01 microM) greater than or equal to 12-deoxy LTB4 (Ki = 8.9 +/- 0.01 microM). Tritium 48-50 prostaglandin reductase 1 Cavia porcellus 76-80 2849666-5 1988 In competition studies for the displacement of [3H]LTB4 from binding sites, LTB4 and its analogs had the following order of potency: LTB4 (Ki = 2.9 +/- 0.8 nM) greater than 5-epi LTB4 (Ki = 58.7 +/- 0.3 nM) greater than 5-deoxy-LTB4 (Ki = 91.7 +/- 0.3 nM) greater than 12-epi LTB4 (Ki = 4.7 +/- 1.2 microM) greater than 5,12-deoxy LTB4 (Ki = 7.6 +/- 0.01 microM) greater than or equal to 12-deoxy LTB4 (Ki = 8.9 +/- 0.01 microM). Tritium 48-50 prostaglandin reductase 1 Cavia porcellus 76-80 2849666-5 1988 In competition studies for the displacement of [3H]LTB4 from binding sites, LTB4 and its analogs had the following order of potency: LTB4 (Ki = 2.9 +/- 0.8 nM) greater than 5-epi LTB4 (Ki = 58.7 +/- 0.3 nM) greater than 5-deoxy-LTB4 (Ki = 91.7 +/- 0.3 nM) greater than 12-epi LTB4 (Ki = 4.7 +/- 1.2 microM) greater than 5,12-deoxy LTB4 (Ki = 7.6 +/- 0.01 microM) greater than or equal to 12-deoxy LTB4 (Ki = 8.9 +/- 0.01 microM). Tritium 48-50 prostaglandin reductase 1 Cavia porcellus 76-80 2849666-7 1988 [3H]LTB4 was not metabolized during the binding process as determined by reverse-phase high performance liquid chromatography. Tritium 1-3 prostaglandin reductase 1 Cavia porcellus 4-8 2851117-1 1988 In vitro quantitative receptor autoradiography was performed on frozen sections of rat and human brain to visualize delta opiate receptors using the specific ligand [3H][D-Pen2, D-Pen5]enkephalin. Tritium 166-168 presenilin enhancer, gamma-secretase subunit Homo sapiens 172-176 2851117-5 1988 Sites labelled with [3H][D-Pen2,D-Pen5]enkephalin were distributed heterogeneously within the layers of the frontal and parietal cerebral cortex, with high densities in the superficial and deep cortical layers. Tritium 21-23 presenilin enhancer, gamma-secretase subunit Homo sapiens 27-31 2854190-5 1988 Compared to pair-fed controls, specific 3H-VKA binding was reduced by 13% to 32% in dorsal and ventral hippocampal CA3 stratum lucidum, entorhinal cortex and cerebellum of 45-day-old rats whose mothers consumed either 3.35% or 6.7% ethanol diets. Tritium 40-42 carbonic anhydrase 3 Rattus norvegicus 115-118 2907120-0 1988 Identification of 3H-bradykinin binding sites in PC-12 cells and brain. Tritium 18-20 kininogen 1 Bos taurus 21-31 1974094-1 1990 Variation in the extra- and intra-cellular concentration of calcium ([Ca]e and [Ca]i) affected the 3H-thymidine labeling pattern of sorted S-phase cells in human epidermal cultures. Tritium 99-101 carbonic anhydrase 1 Homo sapiens 80-84 7580259-8 1995 Purified recombinant ClpC facilitated the degradation of 3H-methylcasein by E. coli ClpP in an ATP-dependent fashion. Tritium 57-59 clpP Nicotiana tabacum 84-88 34391031-7 2021 Of these, 13 and 30g, with an 8-benzo(d)thiazol-2-yl-substituted quinazolin-4(3H)-one structure, showed optimal activity (h. PAR4-AP PRP IC50 = 19.6 nM and 6.59 nM, respectively) on human platelets. Tritium 78-80 F2R like thrombin or trypsin receptor 3 Homo sapiens 125-129 5783958-0 1969 Specific tritium labelling of a potent gastrin analogue. Tritium 9-16 gastrin Homo sapiens 39-46 34098144-4 2021 METHODS: ABA binding to human recombinant LANCL1 was explored by equilibrium binding experiments with (3H)ABA, by circular dichroism and by surface plasmon resonance. Tritium 103-105 LanC like 1 Homo sapiens 42-48 2459224-4 1988 Experiments were conducted to determine if PC1-F could sensitize normal mast cells in vitro for subsequent release of 3H-5-HT that had been taken up previously. Tritium 118-120 minisatellite 6 hypermutable Mus musculus 43-46 3139989-7 1988 Binding of [3H]5-HT to 5-HT1C sites in rat and bovine brain and choroid plexus is relatively insensitive to NEM. Tritium 12-14 5-hydroxytryptamine receptor 2C Rattus norvegicus 23-29 5916390-2 1966 Preparation of radioactive, sialic acid-free ceruloplasmin labeled with tritium on terminal D-galactose residues. Tritium 72-79 ceruloplasmin Homo sapiens 45-58 2456915-13 1988 [3H]cAMP binding to all cytosol fractions decreased as PTH increased over the concentration range predicted by our PKA activation experiments. Tritium 1-3 KIT proto-oncogene receptor tyrosine kinase Rattus norvegicus 115-118 34528988-6 2021 The KOR affinity was determined in receptor binding studies with the radioligand (3H)U-69 593. Tritium 82-84 opioid receptor kappa 1 Homo sapiens 4-7 33369690-7 2021 RESULTS: [3H]MODAG-001 revealed a very high affinity towards pure alphaSYN fibrils (Kd = 0.6 +- 0.1 nM) and only a moderate affinity to hTau46 fibrils (Kd = 19 +- 6.4 nM) as well as amyloid-beta1-42 fibrils (Kd = 20 +- 10 nM). Tritium 10-12 synuclein, alpha Mus musculus 66-74 34288692-4 2021 Docking studies with the FLT3 kinase showed a type I binding mode, where the 3H-pyrazolo group interacts with Cys694 in the hinge region. Tritium 77-80 FMS-like tyrosine kinase 3 Mus musculus 25-29 34195432-4 2021 The inhibition constant (Ki) for l-menthol inhibition of binding of (3H)-WIN35,428 to the human recombinant dopamine transporter was 6.15 x 10-4 mol/L. Tritium 69-71 solute carrier family 6 member 3 Homo sapiens 108-128 3262189-2 1988 In the absence of molybdate, cytosol [3H]R1881-androgen receptor complexes were mainly eluted in the 1.3 M region (Peak 1) with a small peak in the 0.8 M region (Peak 2) of a descending salt gradient (2 to 0 M) of ammonium sulfate. Tritium 38-40 androgen receptor Rattus norvegicus 47-64 2841557-4 1988 CCK (50 nmol/L) alone increases phosphoinositide (PI) hydrolysis in islets, an event reflected by an increase in 3H efflux from myo[2-3H]inositol prelabeled islets and parallel accumulations of labeled inositol phosphates. Tritium 113-115 cholecystokinin Rattus norvegicus 0-3 2974815-5 1988 Further evidence for an action of the adrenal steroids through the androgen receptor is indicated by competition of [3H]testosterone uptake in the tumor cells at the following IC50 values: 0.21 nM, 0.63 nM, 50 nM, 75 nM and 680 nM for DHT, testosterone, delta 4-dione, delta 5-diol and DHEA, respectively. Tritium 117-119 androgen receptor Mus musculus 67-84 2852369-0 1988 Characterization of stress-induced alterations in [3H] Ro5-4864 binding to peripheral benzodiazepine receptors in rat heart and kidney. Tritium 51-53 translocator protein Rattus norvegicus 75-110 34029804-4 2021 Molecular docking, in vitro 3H-IAA binding assay, and surface plasmon resonance analysis showed that the R enantiomer was preferentially combined with TIR1-IAA7 (Transport Inhibitor Response1- Auxin-Responsive Protein IAA7) than the S enantiomer. Tritium 28-30 indole-3-acetic acid 7 Arabidopsis thaliana 156-160 2846046-4 1988 Synthetic SFGF (26-80) was found to share about 10% of the activities as EGF in the radioreceptor binding to A431 cells, stimulation of [3H]thymidine uptake in NRK cells, and induction of colony formation in soft-agar assay. Tritium 137-139 gp010L Rabbit fibroma virus 10-14 35526328-3 2022 In this study, we aimed to assess the binding of (3H)UCB-J as a selective radioligand for SV2A to visualize and determine changes during different stages of epileptogenesis by in-vitro autoradiography in rat models of temporal lobe epilepsy. Tritium 50-52 synaptic vesicle glycoprotein 2a Rattus norvegicus 90-94 33300240-4 2021 Distorted tetrahedral NiF 4 ( D 2d ) and trigonal planar NiF 3 ( D 3h ) molecules were produced by thermal evaporation or laser-ablation of nickel atoms in a fluorine/noble gas mixture and spectroscopically identified by a joint matrix-isolation and quantum-chemical study. Tritium 67-69 CTD small phosphatase 1 Homo sapiens 57-62 35624746-6 2022 Statistically significant MAO-B inhibitory effects were exerted by some of the compounds where again the catecholic compound 3h was the most potent inhibitor similar to selegiline and rasagiline. Tritium 125-127 monoamine oxidase B Homo sapiens 26-31 2615570-3 1989 Incubations conducted in the presence of active LCAT led to a time-dependent increase in the amount of the 3H label retained by an anti-apoA-IV immunoaffinity column. Tritium 107-109 lecithin cholesterol acyltransferase Rattus norvegicus 48-52 2597671-2 1989 We analyzed the distribution of [3H]alkylmethoxy-GPC in subcellular membranes isolated from human promyelocytic leukemia (HL-60) cells. Tritium 33-35 glycophorin C (Gerbich blood group) Homo sapiens 49-52 2597671-3 1989 [3H]Alkylmethyl-GPC added to intact cells selectively labels plasma membrane-enriched fractions of the postnuclear supernatant, but the labeling profile is independent of the temperature and duration of the incubation, and concentration of the molecule. Tritium 1-3 glycophorin C (Gerbich blood group) Homo sapiens 16-19 3179614-2 1988 The dissociation of [3H]-(+)-N-methyl-4-methyldiphenhydramine ([3H]-QMDP) from the histamine H1-receptor was markedly temperature-dependent. Tritium 21-23 histamine H1 receptor Cavia porcellus 83-104 3179614-2 1988 The dissociation of [3H]-(+)-N-methyl-4-methyldiphenhydramine ([3H]-QMDP) from the histamine H1-receptor was markedly temperature-dependent. Tritium 64-66 histamine H1 receptor Cavia porcellus 83-104 2898362-8 1988 A specific, reversible and saturable binding of [3H]PAF to membrane preparations of rat hypothalamus was demonstrated and two classes of binding sites were characterized. Tritium 49-51 PCNA clamp associated factor Rattus norvegicus 52-55 2455904-4 1988 The onset of the PRL stimulation of [3H]glucose incorporation into lactose occurred 6-8 hr after exposing the explants to PRL. Tritium 37-39 prolactin Mus musculus 17-20 2455904-4 1988 The onset of the PRL stimulation of [3H]glucose incorporation into lactose occurred 6-8 hr after exposing the explants to PRL. Tritium 37-39 prolactin Mus musculus 122-125 2597671-5 1989 Moreover, [3H]alkylmethyl-GPC translocates between subcellular membranes in a manner that does not depend on membrane-adsorbed or cytosolic transfer proteins. Tritium 11-13 glycophorin C (Gerbich blood group) Homo sapiens 26-29 32696514-5 2020 Compounds 3f and 3h exhibited COX-1 inhibition, with IC50 values of 1,485 and 684 nM, respectively. Tritium 17-19 mitochondrially encoded cytochrome c oxidase I Homo sapiens 30-35 2530275-10 1989 Furthermore, MGF, pTGF-beta 1, and pTGF-beta 2 also decreased IL-2 mediated [3H]thymidine incorporation into human PBL Con A blasts and the IL-4-mediated [3H]thymidine incorporation of purified T lymphocytes costimulated with PDBu by 70%. Tritium 155-157 growth differentiation factor 15 Homo sapiens 18-27 2530275-10 1989 Furthermore, MGF, pTGF-beta 1, and pTGF-beta 2 also decreased IL-2 mediated [3H]thymidine incorporation into human PBL Con A blasts and the IL-4-mediated [3H]thymidine incorporation of purified T lymphocytes costimulated with PDBu by 70%. Tritium 155-157 growth differentiation factor 15 Homo sapiens 35-44 2533325-4 1989 In addition, 5-HT1A and 5-HT1C sites were directly visualized with the more selective radioligands [3H]8-OH-DPAT and [3H]mesulergine, respectively. Tritium 100-103 5-hydroxytryptamine receptor 2C Homo sapiens 24-30 2968796-3 1988 The two proteins were strong inhibitors of phospholipase A2 as assessed in vitro with porcine pancreatic phospholipase A2 and [3H]-oleic acid labeled E. Coli membranes as substrate. Tritium 127-129 phospholipase A2, group IB, pancreas Mus musculus 43-59 32237096-1 2020 Starting from readily available o-diazoacyl-substituted arene carboxylates, scaffolds with the 5,9-epoxycyclohepta[b]pyran-2(3H)-one core were obtained by cooperative Rh(II), Lewis and Bronsted acid catalysis. Tritium 125-127 Rh blood group D antigen Homo sapiens 167-173 3170521-1 1988 The [3H]triamcinolone acetonide ([3H]TA)-binding ability of the rat liver glucocorticoid receptor (GR) was investigated under acidic conditions, ranging from pH 2 to 7.3. Tritium 4-7 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 74-97 3170521-1 1988 The [3H]triamcinolone acetonide ([3H]TA)-binding ability of the rat liver glucocorticoid receptor (GR) was investigated under acidic conditions, ranging from pH 2 to 7.3. Tritium 4-7 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 99-101 3170521-4 1988 Moreover, in the absence of molybdate, the [3H]TA-GR complexes formed at pH 7.3 remained unchanged until pH 5. Tritium 44-46 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 50-52 3170521-7 1988 These results suggest that rat liver GR is rather resistant under acidic conditions and that it exists in a peculiar state below pH 5.9 to approximately 4 as to its ligand binding property: unoccupied GR has no [3H]TA-binding ability but [3H]TA-GR complexes once formed at neutral pH do not dissociate. Tritium 239-241 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 37-39 3170521-7 1988 These results suggest that rat liver GR is rather resistant under acidic conditions and that it exists in a peculiar state below pH 5.9 to approximately 4 as to its ligand binding property: unoccupied GR has no [3H]TA-binding ability but [3H]TA-GR complexes once formed at neutral pH do not dissociate. Tritium 239-241 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 201-203 3170521-7 1988 These results suggest that rat liver GR is rather resistant under acidic conditions and that it exists in a peculiar state below pH 5.9 to approximately 4 as to its ligand binding property: unoccupied GR has no [3H]TA-binding ability but [3H]TA-GR complexes once formed at neutral pH do not dissociate. Tritium 239-241 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 201-203 2583526-7 1989 The difference could be due to decreased glycosylation of the vaccine strain E2 protein, as revealed by [3H]mannose incorporation studies. Tritium 105-107 ubiquitin conjugating enzyme E2 B Homo sapiens 77-87 2478032-7 1989 Both CCK-8 and the analogue stimulated [3H]leucine incorporation into protein at low concentrations in rat pancreatic acini. Tritium 40-42 cholecystokinin Rattus norvegicus 5-8 2478032-10 1989 In mouse acini, however, the CCK analogue inhibited [3H]leucine incorporation similar to the effect of CCK-8. Tritium 53-55 cholecystokinin Mus musculus 29-32 3170521-8 1988 [3H]TA-GR complexes recovered from the precipitate at pH 5 had a Stokes radius of 7.5 nm, little DNA-cellulose-binding ability and sedimented at 8.6S on glycerol gradient centrifugation, indicating that the receptor existed in a nontransformed state. Tritium 1-3 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 7-9 31721581-8 2020 [3H]6 constitutes the first GPR84 agonist radioligand representing a powerful tool for this poorly investigated GPCR, which has potential as a future drug target. Tritium 1-3 C-X-C motif chemokine receptor 6 Homo sapiens 112-116 3399126-6 1988 Under these conditions, 5-HT1D sites represented a high proportion of total [3H]5-HT binding sites in the basal ganglia and substantia nigra. Tritium 77-79 5-hydroxytryptamine receptor 1D Homo sapiens 24-30 2833347-9 1988 The functions of the Na+/K+ and Na+/H+ exchange systems are apparently necessary for the enhanced A system activity, since ouabain and amiloride can inhibit the uptake of [3H]methylaminoisobutyric acid in K-NRK cells and in NRK cells treated with TGF-alpha and/or -beta. Tritium 172-174 transforming growth factor alpha Rattus norvegicus 247-256 2505676-1 1989 Incubation of rabbit platelets with thrombin resulted in rapid accumulations of inositol trisphosphate (IP3) in [3H]inositol-labeled platelets, increases of [3H]arachidonic acid [( 3H]AA) release, and [3H]serotonin secretion from the platelets prelabeled with these labeled compounds. Tritium 113-115 prothrombin Oryctolagus cuniculus 36-44 2505676-1 1989 Incubation of rabbit platelets with thrombin resulted in rapid accumulations of inositol trisphosphate (IP3) in [3H]inositol-labeled platelets, increases of [3H]arachidonic acid [( 3H]AA) release, and [3H]serotonin secretion from the platelets prelabeled with these labeled compounds. Tritium 158-160 prothrombin Oryctolagus cuniculus 36-44 2505676-1 1989 Incubation of rabbit platelets with thrombin resulted in rapid accumulations of inositol trisphosphate (IP3) in [3H]inositol-labeled platelets, increases of [3H]arachidonic acid [( 3H]AA) release, and [3H]serotonin secretion from the platelets prelabeled with these labeled compounds. Tritium 158-160 prothrombin Oryctolagus cuniculus 36-44 2505676-1 1989 Incubation of rabbit platelets with thrombin resulted in rapid accumulations of inositol trisphosphate (IP3) in [3H]inositol-labeled platelets, increases of [3H]arachidonic acid [( 3H]AA) release, and [3H]serotonin secretion from the platelets prelabeled with these labeled compounds. Tritium 158-160 prothrombin Oryctolagus cuniculus 36-44 2793471-5 1989 The average 3H concentration in the free water including tap water was 1.6 Bq L-1. Tritium 12-14 nuclear RNA export factor 1 Homo sapiens 57-60 3282875-5 1988 IGF-I and IGF-II also increased [3H]thymidine incorporation into PC12 cells incubated in low serum medium. Tritium 33-35 insulin-like growth factor 2 Rattus norvegicus 10-16 2833177-11 1988 A higher ratio of 3H/14C in the cytochrome P-450d from isosafrole + TCDD- and HCB + TCDD-treated rats relative to TCDD (control)-treated rats suggested that isosafrole and HCB were able to retard the degradation of cytochrome P-450d, presumably by virtue of being tightly bound to the enzyme. Tritium 18-20 cytochrome P450, family 1, subfamily a, polypeptide 2 Rattus norvegicus 32-49 33132311-7 2020 In addition, co-administration of substrates/inhibitors for Oat3, Oatp1a4, and/or Mrp4, such as benzylpenicillin and cefmetazole, reduced [3H]PGD2 elimination across the BBB. Tritium 139-141 solute carrier family 22 member 8 Rattus norvegicus 60-64 3382028-0 1988 Androgen receptor-deficient Tfm cells in the mosaic epididymis of sex-reversed mice heterozygous for Tfm: an autoradiographic study with [3H]-dihydrotestosterone and [3H]-estradiol. Tritium 138-142 androgen receptor Mus musculus 0-17 3382028-0 1988 Androgen receptor-deficient Tfm cells in the mosaic epididymis of sex-reversed mice heterozygous for Tfm: an autoradiographic study with [3H]-dihydrotestosterone and [3H]-estradiol. Tritium 138-141 androgen receptor Mus musculus 0-17 2530468-7 1989 A small proportion (less than 10%) of [3H]5-HT sites in the frontal cortex appeared to be of the 5-HT1C subtype, as these sites were displaced with high affinity by mianserin. Tritium 39-41 5-hydroxytryptamine receptor 2C Homo sapiens 97-103 30734226-4 2019 Incubation of synaptosomes with the anti-GluN1, the anti-GluN2A, the anti-GluN2B, or the anti-GluN3A antibody prevented the 30 muM NMDA/1 muM glycine-evoked [3H]D-aspartate ([3H]D-ASP) release. Tritium 158-160 glutamate receptor, ionotropic, NMDA1 (zeta 1) Mus musculus 41-46 2507360-1 1989 gamma-Thrombin stimulated release of [3H]arachidonic acid ([3H]AA) accompanied by a significant production of PAF and lyso-PAF by rabbit platelets. Tritium 38-40 prothrombin Oryctolagus cuniculus 6-14 2507360-4 1989 However, addition of ionophore A23187 enhanced the release of [3H]AA by gamma-thrombin to the levels similar to those of alpha-thrombin. Tritium 63-65 prothrombin Oryctolagus cuniculus 78-86 2550427-2 1989 Treatment of cells with 0.5 nM 1,25-(OH)2[3H]D3 for 16 h results in up-regulation of total cell VDR from 82 to 170 fmol/mg protein as measured in a cytosol binding assay. Tritium 42-44 vitamin D receptor Homo sapiens 96-99 3382028-6 1988 After injection of [3H]estradiol, both Tfm and wild-type cells show nuclear labeling. Tritium 20-22 androgen receptor Mus musculus 39-42 3422006-2 1988 Synthesis of tritium-labelled prostaglandins A1, A2, B1, B2, F1 alpha, F2 alpha, F1 beta, F2 beta of high molar radioactivity from highly labelled PGE1 and PGE2 is described. Tritium 13-20 immunoglobulin kappa variable 5-2 Homo sapiens 57-97 2450171-7 1988 The MBPs of rat, rabbit, pig, and cow also inhibit [3H]TMA binding noncompetitively with similar potencies. Tritium 52-54 myelin basic protein Rattus norvegicus 4-8 2450171-9 1988 Studies of fragments of rabbit MBP suggest that the determinants of affinity for the [3H]TMA site are contained primarily within the C-terminal half of the rabbit MBP. Tritium 86-88 myelin basic protein Oryctolagus cuniculus 31-34 2550427-9 1989 In addition, the presence of competitive inhibitors amplified responses of cells to 1,25-(OH)2[3H]D3 treatment as measured by an increased occupancy of VDR by 1,25-(OH)2[3H]D3 and increased up-regulation of VDR over that observed without metabolism inhibitors. Tritium 95-97 vitamin D receptor Homo sapiens 152-155 30734226-4 2019 Incubation of synaptosomes with the anti-GluN1, the anti-GluN2A, the anti-GluN2B, or the anti-GluN3A antibody prevented the 30 muM NMDA/1 muM glycine-evoked [3H]D-aspartate ([3H]D-ASP) release. Tritium 158-160 glutamate receptor, ionotropic, NMDA2A (epsilon 1) Mus musculus 57-63 2550427-9 1989 In addition, the presence of competitive inhibitors amplified responses of cells to 1,25-(OH)2[3H]D3 treatment as measured by an increased occupancy of VDR by 1,25-(OH)2[3H]D3 and increased up-regulation of VDR over that observed without metabolism inhibitors. Tritium 95-97 vitamin D receptor Homo sapiens 207-210 2550427-9 1989 In addition, the presence of competitive inhibitors amplified responses of cells to 1,25-(OH)2[3H]D3 treatment as measured by an increased occupancy of VDR by 1,25-(OH)2[3H]D3 and increased up-regulation of VDR over that observed without metabolism inhibitors. Tritium 170-172 vitamin D receptor Homo sapiens 152-155 2450171-9 1988 Studies of fragments of rabbit MBP suggest that the determinants of affinity for the [3H]TMA site are contained primarily within the C-terminal half of the rabbit MBP. Tritium 86-88 myelin basic protein Oryctolagus cuniculus 163-166 2450171-11 1988 The identification of the endogenous inhibitor of [3H]TMA binding as MBP suggests that MBP interacts physiologically with the high-affinity cannabinoid site. Tritium 51-53 myelin basic protein Rattus norvegicus 69-72 2450171-11 1988 The identification of the endogenous inhibitor of [3H]TMA binding as MBP suggests that MBP interacts physiologically with the high-affinity cannabinoid site. Tritium 51-53 myelin basic protein Rattus norvegicus 87-90 30734226-4 2019 Incubation of synaptosomes with the anti-GluN1, the anti-GluN2A, the anti-GluN2B, or the anti-GluN3A antibody prevented the 30 muM NMDA/1 muM glycine-evoked [3H]D-aspartate ([3H]D-ASP) release. Tritium 158-160 glutamate receptor, ionotropic, NMDA2B (epsilon 2) Mus musculus 74-80 30734226-4 2019 Incubation of synaptosomes with the anti-GluN1, the anti-GluN2A, the anti-GluN2B, or the anti-GluN3A antibody prevented the 30 muM NMDA/1 muM glycine-evoked [3H]D-aspartate ([3H]D-ASP) release. Tritium 175-177 glutamate receptor, ionotropic, NMDA1 (zeta 1) Mus musculus 41-46 2556108-1 1989 The effects of the muscarinic agonist carbachol, histamine and bradykinin on incorporation of [3H]inositol into the phosphoinositides and the formation of [3H]InsPs were examined in bovine tracheal smooth-muscle (BTSM) slices labelled with [3H]inositol. Tritium 95-97 kininogen 1 Bos taurus 63-73 3126263-5 1988 The presence of the irreversible MAO-B inhibitor l-deprenyl completely abolished the irreversible labelling of the membranes by [3H]Ro 16-6491. Tritium 129-131 monoamine oxidase B Homo sapiens 33-38 30734226-4 2019 Incubation of synaptosomes with the anti-GluN1, the anti-GluN2A, the anti-GluN2B, or the anti-GluN3A antibody prevented the 30 muM NMDA/1 muM glycine-evoked [3H]D-aspartate ([3H]D-ASP) release. Tritium 175-177 glutamate receptor, ionotropic, NMDA2A (epsilon 1) Mus musculus 57-63 3126263-6 1988 The selective inactivation of MAO-B, e.g., by l-deprenyl prevented the covalent incorporation of [3H]Ro 16-6491 whereas selective inhibition of the MAO-A by clorgyline was without effect. Tritium 98-100 monoamine oxidase B Homo sapiens 30-35 3126263-9 1988 Our results indicate that the polypeptide that is covalently labelled by [3H]Ro 16-6491 corresponds to one of the two MAO-B subunits. Tritium 74-76 monoamine oxidase B Homo sapiens 118-123 3126267-5 1988 Inhibition of [3H]choline uptake by arachidonic acid was reversed by bovine serum albumin. Tritium 15-17 albumin Rattus norvegicus 76-89 3259151-5 1988 Saturation analysis of anterior pituitary and brain tissue (pool of hypothalamic, preoptic area, amygdala and septum remaining after microdissection of brain nuclei) showed that [3H]R1881 binds to the androgen receptor with high specificity and affinity (Kd = 1.25 x 10(-10) M, 0.45 x 10(-10) M, in anterior pituitary and HPA cytosol, respectively). Tritium 179-181 androgen receptor Rattus norvegicus 201-218 2832168-9 1988 In contrast with many previous unsuccessful attempts, the separation of the C57BL/6 Ah receptor allowed the unambiguous detection of a 9-S binding peak with [3H]benzo[a]pyrene as a radioligand. Tritium 158-160 aryl hydrocarbon receptor Rattus norvegicus 84-95 2774562-6 1989 Crude alkaline phosphatase (containing some protease activity) substantially reduced the ability of glucocorticoid receptor to bind hormone and shifted the sedimentation position of the glucocorticoid receptor from approximately 8 S to approximately 2 S. Crude alkaline phosphatase did not reduce the ability of Ah receptor to bind [3H]TCDD and did not alter sedimentation of the 9 S [3H]TCDD. Tritium 333-335 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 100-123 2774562-6 1989 Crude alkaline phosphatase (containing some protease activity) substantially reduced the ability of glucocorticoid receptor to bind hormone and shifted the sedimentation position of the glucocorticoid receptor from approximately 8 S to approximately 2 S. Crude alkaline phosphatase did not reduce the ability of Ah receptor to bind [3H]TCDD and did not alter sedimentation of the 9 S [3H]TCDD. Tritium 333-335 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 186-209 2774562-6 1989 Crude alkaline phosphatase (containing some protease activity) substantially reduced the ability of glucocorticoid receptor to bind hormone and shifted the sedimentation position of the glucocorticoid receptor from approximately 8 S to approximately 2 S. Crude alkaline phosphatase did not reduce the ability of Ah receptor to bind [3H]TCDD and did not alter sedimentation of the 9 S [3H]TCDD. Tritium 385-387 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 100-123 2774562-6 1989 Crude alkaline phosphatase (containing some protease activity) substantially reduced the ability of glucocorticoid receptor to bind hormone and shifted the sedimentation position of the glucocorticoid receptor from approximately 8 S to approximately 2 S. Crude alkaline phosphatase did not reduce the ability of Ah receptor to bind [3H]TCDD and did not alter sedimentation of the 9 S [3H]TCDD. Tritium 385-387 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 186-209 2760618-3 1989 By contrast, [3H]NECA, a nonselective A-1/A-2 ligand, gave 72% specific binding of the total binding. Tritium 14-16 UDP glucuronosyltransferase family 1 member A6 Rattus norvegicus 38-41 30734226-4 2019 Incubation of synaptosomes with the anti-GluN1, the anti-GluN2A, the anti-GluN2B, or the anti-GluN3A antibody prevented the 30 muM NMDA/1 muM glycine-evoked [3H]D-aspartate ([3H]D-ASP) release. Tritium 175-177 glutamate receptor, ionotropic, NMDA2B (epsilon 2) Mus musculus 74-80 2828552-4 1988 Covalent labeling studies (with [3H]propylbenzilycholine mustard) suggest that the size of the SK-N-SH mAChR (Mr = 81,000-98,000) distinguishes it from the predominant mAChR species in rat cerebral cortex (Mr = 66,000), an M1-enriched tissue. Tritium 33-35 hedgehog acyltransferase Homo sapiens 95-99 30734226-5 2019 The NMDA/glycine-evoked [3H]D-ASP release was reduced by increasing the external protons, consistent with the participation of GluN1 subunits lacking the N1 cassette to the receptor assembly. Tritium 25-27 glutamate receptor, ionotropic, NMDA1 (zeta 1) Mus musculus 127-132 31093828-7 2019 Phosphorylation and transport of OATP1B1 was determined using 32P-orthophosphate labeling and [3H]estradiol-17beta-glucuronide accumulation, respectively. Tritium 95-97 solute carrier organic anion transporter family member 1B1 Homo sapiens 33-40 3339364-1 1988 Analysis of the equilibrium binding of [3H]-neurotensin(1-13) at 25 degrees C to its receptor sites in bovine cortex membranes indicated a single population of sites with an apparent equilibrium dissociation constant (KD) of 3.3 nM and a density (Bmax) of 350 fmol/mg protein (Hill coefficient nH = 0.97). Tritium 40-42 neurotensin Bos taurus 44-55 3339364-4 1988 The binding of [3H]neurotensin was decreased by cations (monovalent and divalent) and by a nonhydrolysable guanine nucleotide analogue. Tritium 16-18 neurotensin Bos taurus 19-30 3339364-7 1988 Stable binding activity for [3H]neurotensin in detergent solution (Kd = 5.5 nM, Bmax = 250 fmol/mg protein, nH = 1.0) was obtained in 2% digitonin/1 mM Mg2+ extracts of membranes which had been preincubated (25 degrees C, 1 h) with 1 mM Mg2+ prior to solubilization. Tritium 29-31 neurotensin Bos taurus 32-43 3339364-9 1988 The solubilized [3H]-neurotensin activity retained its sensitivity to cations and guanine nucleotide. Tritium 17-19 neurotensin Bos taurus 21-32 2512511-4 1989 +/- CYP affinity for 5-HT1B sites labeled by [3H]dihydroergotamine [( 3H]DE) also displays a small but significant reduction (from Ki = 1.4 nM to Ki = 3.5nM) by the inclusion of 100 microM GTP. Tritium 46-48 5-hydroxytryptamine receptor 1B Rattus norvegicus 21-27 2812250-7 1989 Nicotinic binding measured with [3H]ACh, but not (-)-[3H]Nic, was significantly lower in the H2 (field of Rose) and H1-subiculum areas of DAT samples compared to control. Tritium 33-35 solute carrier family 6 member 3 Homo sapiens 138-141 2787989-3 1989 Half maximal stimulation of [3H]thymidine incorporation was seen with 8ng/ml EGF and 7 ng/ml TGFa, respectively. Tritium 29-31 transforming growth factor alpha Rattus norvegicus 93-97 31304000-2 2019 Among these, 3d, 3e, 3g, and 3h were established as the most potent and selective BChE inhibitors (IC50 = 0.5-3.9 muM), while 3f presented dual inhibitory activity against BChE and AChE (IC50 = 6.0 and 6.5 muM, respectively). Tritium 29-31 butyrylcholinesterase Homo sapiens 82-86 2471705-12 1989 The FDC-6-nonreactive synthetic peptide containing the VTHPGY sequence was converted into FDC-6-reactive form on incubation with alpha-N-acetylgalactosaminyltransferase and UDP-[3H]GalNAc in the homogenate of hepatoma cell HUH-7, human fetal fibroblast cell line WI-38, or human epidermoid carcinoma cell line A431. Tritium 177-180 MIR7-3 host gene Homo sapiens 223-228 2721453-6 1989 Analysis of [3H]mannose-labeled oligosaccharides on Concanavalin-A-agarose showed 85% binding for those from TSH alpha, 70% for free alpha, and 50% for those from TSH beta. Tritium 13-15 glycoprotein hormones, alpha subunit Mus musculus 109-118 2828916-3 1988 The results of the present study indicate that 6-methyl-1,3,8-trichlorodibenzofuran (MCDF) effectively competes with [3H]TCDD for binding to the Ah receptor in rat liver cytosol. Tritium 118-120 aryl hydrocarbon receptor Rattus norvegicus 145-156 2462417-4 1988 SP(1-4) also inhibited the electrically stimulated [3H]ACh outflow (presynaptic effect), whereas tuftsin did not affect the ACh outflow. Tritium 52-54 Sp1 transcription factor Rattus norvegicus 0-6 31304000-2 2019 Among these, 3d, 3e, 3g, and 3h were established as the most potent and selective BChE inhibitors (IC50 = 0.5-3.9 muM), while 3f presented dual inhibitory activity against BChE and AChE (IC50 = 6.0 and 6.5 muM, respectively). Tritium 29-31 butyrylcholinesterase Homo sapiens 172-176 3352243-1 1988 The stabilities of tritium-labeled T-2, HT-2, and T-2 tetraol were studied in blood and urine at -70 degrees, 4 degrees, and 23 degrees C for 6 months in the presence of EDTA or NaF. Tritium 19-26 solute carrier family 25 member 5 Homo sapiens 35-38 3422634-5 1988 PGD2 partially inhibits 3H-PGE2 binding, whereas PGA2 does not compete. Tritium 24-26 prostaglandin D2 synthase (brain) Mus musculus 0-4 2826958-1 1988 The characteristics of [3H]Ro 5-4864 binding to "peripheral" benzodiazepine receptors (PBR) in the central nervous system and peripheral tissues were examined after chemical sympathectomy with 6-hydroxydopamine (6-OHDA). Tritium 24-26 translocator protein Homo sapiens 48-85 2826958-1 1988 The characteristics of [3H]Ro 5-4864 binding to "peripheral" benzodiazepine receptors (PBR) in the central nervous system and peripheral tissues were examined after chemical sympathectomy with 6-hydroxydopamine (6-OHDA). Tritium 24-26 translocator protein Homo sapiens 87-90 2542068-7 1989 Since Gs alpha photolabeling was inversely proportional to the percentage of [3H]thymidine-labeled nuclei at confluence, we suggest that the altered Gs alpha in tumor cells is involved in the loss of cell growth regulation. Tritium 78-80 GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus Mus musculus 149-157 2641350-1 1989 This study documents the role of mandibular epithelium and epidermal growth factor (EGF) in the initiation, maturation and maintenance of Meckel"s cartilage using percent 3H-thymidine-labelled cells as an index of proliferative activity and distribution of labelled cells, chondrocyte size and relative amount of extracellular matrix as indices of chondrogenesis. Tritium 171-173 epidermal growth factor Gallus gallus 84-87 2733696-0 1989 Characterization of the binding of [3H]L-365,260: a new potent and selective brain cholecystokinin (CCK-B) and gastrin receptor antagonist radioligand. Tritium 36-38 cholecystokinin Cavia porcellus 83-98 2733696-0 1989 Characterization of the binding of [3H]L-365,260: a new potent and selective brain cholecystokinin (CCK-B) and gastrin receptor antagonist radioligand. Tritium 36-38 cholecystokinin Cavia porcellus 100-103 2733696-1 1989 [3H]L-365,260, [(3R-(+)-2,3-dihydro-1-methyl-2-oxo-5-phenyl-1H-1,4- benzodiazepin-3-yl)-N"-(3-methylphenyl)urea], a new potent and selective nonpeptide brain cholecystokinin (CCK-B) and gastrin receptor antagonist, bound saturably and reversibly to guinea pig brain membranes. Tritium 1-3 cholecystokinin Cavia porcellus 158-173 2733696-1 1989 [3H]L-365,260, [(3R-(+)-2,3-dihydro-1-methyl-2-oxo-5-phenyl-1H-1,4- benzodiazepin-3-yl)-N"-(3-methylphenyl)urea], a new potent and selective nonpeptide brain cholecystokinin (CCK-B) and gastrin receptor antagonist, bound saturably and reversibly to guinea pig brain membranes. Tritium 1-3 cholecystokinin Cavia porcellus 175-178 2733696-4 1989 The relative potencies of various CCK/gastrin-related peptides and nonpeptide peripheral CCK-A antagonists in displacing [3H]L-365,260 brain binding correlated with their potencies in displacing the binding of 125I-CCK to brain receptors but not their potencies in displacing the peripherally selective CCK-A ligand [3H]L-364,718 from pancreatic receptors. Tritium 122-124 cholecystokinin Cavia porcellus 34-37 30820175-9 2019 [3H]EBOB binding data showed that gamma-BHC, its analogs, dieldrin, and other cyclodiene insecticides interact with the same site on GABA receptor as picrotoxinin. Tritium 1-3 GABA type A receptor-associated protein Homo sapiens 133-146 2733696-4 1989 The relative potencies of various CCK/gastrin-related peptides and nonpeptide peripheral CCK-A antagonists in displacing [3H]L-365,260 brain binding correlated with their potencies in displacing the binding of 125I-CCK to brain receptors but not their potencies in displacing the peripherally selective CCK-A ligand [3H]L-364,718 from pancreatic receptors. Tritium 122-124 cholecystokinin Cavia porcellus 89-92 2733696-4 1989 The relative potencies of various CCK/gastrin-related peptides and nonpeptide peripheral CCK-A antagonists in displacing [3H]L-365,260 brain binding correlated with their potencies in displacing the binding of 125I-CCK to brain receptors but not their potencies in displacing the peripherally selective CCK-A ligand [3H]L-364,718 from pancreatic receptors. Tritium 122-124 cholecystokinin Cavia porcellus 89-92 2733696-4 1989 The relative potencies of various CCK/gastrin-related peptides and nonpeptide peripheral CCK-A antagonists in displacing [3H]L-365,260 brain binding correlated with their potencies in displacing the binding of 125I-CCK to brain receptors but not their potencies in displacing the peripherally selective CCK-A ligand [3H]L-364,718 from pancreatic receptors. Tritium 122-124 cholecystokinin Cavia porcellus 89-92 2733696-5 1989 The regional distribution of [3H]L-365,260 binding in various brain areas correlated with 125I-CCK binding. Tritium 30-32 cholecystokinin Cavia porcellus 95-98 2733696-8 1989 The binding of [3H]L-365,260 to gastric glands was inhibited by CCK/gastrin antagonists with potencies similar to those for inhibition of 125I-gastrin binding in this tissue. Tritium 16-18 cholecystokinin Cavia porcellus 64-67 2733696-9 1989 Collectively, the data indicates that [3H]L-365,260 represents a new potent nonpeptide antagonist radioligand suitable for the study of brain CCK-B and gastrin receptors. Tritium 39-41 cholecystokinin Cavia porcellus 142-145 2786445-7 1989 In the CA3 subfield, only a small and transient alteration in the [3H]IP3 binding was noticed during recirculation. Tritium 67-69 carbonic anhydrase 3 Rattus norvegicus 7-10 2447487-1 1988 C3H10T1/2 murine fibroblasts overexpressing chicken pp60c-src showed a two- to fivefold enhanced incorporation of [3H]thymidine into DNA in response to epidermal growth factor (EGF) relative to that of the parent line. Tritium 1-3 SRC proto-oncogene, non-receptor tyrosine kinase Gallus gallus 52-61 2447487-1 1988 C3H10T1/2 murine fibroblasts overexpressing chicken pp60c-src showed a two- to fivefold enhanced incorporation of [3H]thymidine into DNA in response to epidermal growth factor (EGF) relative to that of the parent line. Tritium 1-3 epidermal growth factor Gallus gallus 152-175 2447487-1 1988 C3H10T1/2 murine fibroblasts overexpressing chicken pp60c-src showed a two- to fivefold enhanced incorporation of [3H]thymidine into DNA in response to epidermal growth factor (EGF) relative to that of the parent line. Tritium 1-3 epidermal growth factor Gallus gallus 177-180 2827000-5 1988 Washing the pretreated platelets with either buffer or buffer containing 0.5% bovine serum albumin did not restore the PAF for 10 min), platelets remained fully responsive to thrombin (2 units/ml)-stimulated production of [3H]IP3. Tritium 223-225 prothrombin Oryctolagus cuniculus 175-183 2829167-4 1988 Stimulation of islet cell [3H]thymidine incorporation was observed after transfection with src alone or the combination of myc and ras. Tritium 27-29 SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus 91-94 30634395-5 2019 The role of Sp1 in SN1 expression and SN1-mediated [3H]glutamine uptake in ammonia-treated astrocytes was verified using siRNA and mithramycin A. Tritium 52-54 solute carrier family 38, member 3 Mus musculus 38-41 2825924-0 1987 Inhibition of [3H]diazepam binding in primary cultures of astrocytes by atrial natriuretic peptide and by a cyclic GMP analog. Tritium 15-17 5'-nucleotidase, cytosolic II Homo sapiens 115-118 2826763-5 1987 Competition studies with selective drugs reveal alpha adrenergic, 5-HT1A and 5-HT1B components of [3H]DE specific binding. Tritium 99-101 5-hydroxytryptamine receptor 1B Rattus norvegicus 77-83 2826763-7 1987 Under these 5-HT1B-selective conditions, [3H]DE binding is about 90% specific, with a Kd of about 50 to 60 pM and a Bmax of 96 fmol/mg of protein in hippocampus and 77 fmol/mg of protein in cortex. Tritium 42-44 5-hydroxytryptamine receptor 1B Rattus norvegicus 12-18 2826763-8 1987 [3H]DE binding to 5-HT1B sites is very slowly dissociable, with a T1/2 of greater than 2 h at 37 degrees C. 5-HT1B antagonists and DE itself yield competition curves at [3H]DE-labeled 5-HT1B sites that are adequately fit assuming a single site in nonlinear regression analysis. Tritium 1-3 5-hydroxytryptamine receptor 1B Rattus norvegicus 18-24 2826763-8 1987 [3H]DE binding to 5-HT1B sites is very slowly dissociable, with a T1/2 of greater than 2 h at 37 degrees C. 5-HT1B antagonists and DE itself yield competition curves at [3H]DE-labeled 5-HT1B sites that are adequately fit assuming a single site in nonlinear regression analysis. Tritium 1-3 5-hydroxytryptamine receptor 1B Rattus norvegicus 108-114 2826763-8 1987 [3H]DE binding to 5-HT1B sites is very slowly dissociable, with a T1/2 of greater than 2 h at 37 degrees C. 5-HT1B antagonists and DE itself yield competition curves at [3H]DE-labeled 5-HT1B sites that are adequately fit assuming a single site in nonlinear regression analysis. Tritium 1-3 5-hydroxytryptamine receptor 1B Rattus norvegicus 108-114 2826763-8 1987 [3H]DE binding to 5-HT1B sites is very slowly dissociable, with a T1/2 of greater than 2 h at 37 degrees C. 5-HT1B antagonists and DE itself yield competition curves at [3H]DE-labeled 5-HT1B sites that are adequately fit assuming a single site in nonlinear regression analysis. Tritium 170-172 5-hydroxytryptamine receptor 1B Rattus norvegicus 18-24 2826763-8 1987 [3H]DE binding to 5-HT1B sites is very slowly dissociable, with a T1/2 of greater than 2 h at 37 degrees C. 5-HT1B antagonists and DE itself yield competition curves at [3H]DE-labeled 5-HT1B sites that are adequately fit assuming a single site in nonlinear regression analysis. Tritium 170-172 5-hydroxytryptamine receptor 1B Rattus norvegicus 108-114 2826763-8 1987 [3H]DE binding to 5-HT1B sites is very slowly dissociable, with a T1/2 of greater than 2 h at 37 degrees C. 5-HT1B antagonists and DE itself yield competition curves at [3H]DE-labeled 5-HT1B sites that are adequately fit assuming a single site in nonlinear regression analysis. Tritium 170-172 5-hydroxytryptamine receptor 1B Rattus norvegicus 108-114 2724687-2 1989 3H-propionylated CCK-8 [( 3H]CCK-8) was used as a ligand. Tritium 0-2 cholecystokinin Rattus norvegicus 17-20 2724687-2 1989 3H-propionylated CCK-8 [( 3H]CCK-8) was used as a ligand. Tritium 0-2 cholecystokinin Rattus norvegicus 29-32 2724687-7 1989 3) The carboxy-terminal tetra-peptide (CCK-4) and penta-peptide (CCK-5) had very weak potencies in displacing [3H]CCK-8 binding in the pancreatic acini, which were 20 to 30-fold less than their potencies in the cerebral cortex. Tritium 111-113 cholecystokinin Rattus norvegicus 39-42 2724687-7 1989 3) The carboxy-terminal tetra-peptide (CCK-4) and penta-peptide (CCK-5) had very weak potencies in displacing [3H]CCK-8 binding in the pancreatic acini, which were 20 to 30-fold less than their potencies in the cerebral cortex. Tritium 111-113 cholecystokinin Rattus norvegicus 65-68 2724687-7 1989 3) The carboxy-terminal tetra-peptide (CCK-4) and penta-peptide (CCK-5) had very weak potencies in displacing [3H]CCK-8 binding in the pancreatic acini, which were 20 to 30-fold less than their potencies in the cerebral cortex. Tritium 111-113 cholecystokinin Rattus norvegicus 65-68 2544916-1 1989 The inhibitory potencies of imipramine (IC50-values for IMI) and trazodone (IC50-values for TRA) on platelet [3H]serotonin uptake were measured in depressed patients. Tritium 110-112 T cell receptor alpha locus Homo sapiens 92-95 30634395-7 2019 Sp1 translocation to the nuclei and its enhanced binding to the Snat3 promoter, along with Sp1 dependence of system N-mediated [3H]glutamine uptake, were observed in astrocytes upon ammonia exposure. Tritium 128-130 solute carrier family 38, member 3 Mus musculus 64-69 2744079-0 1989 [3H]Ro 19-6327: a reversible ligand and affinity labelling probe for monoamine oxidase-B. Tritium 1-3 monoamine oxidase B Homo sapiens 69-88 2744079-2 1989 This compound is a novel, time-dependent inhibitor of monoamine oxidase type B (MAO-B) and is structurally closely related to [3H]Ro 16-6491. Tritium 127-129 monoamine oxidase B Homo sapiens 54-78 3681301-3 1987 In experiments measuring the time course of [3H]neurotensin degradation, a minimum of six major tritiated products were found, with the breakdown peptides formed and the degree of proteolysis of [3H]neurotensin being dependent upon the length of incubation and the concentration of cells. Tritium 45-47 neurotensin Mus musculus 48-59 2744079-2 1989 This compound is a novel, time-dependent inhibitor of monoamine oxidase type B (MAO-B) and is structurally closely related to [3H]Ro 16-6491. Tritium 127-129 monoamine oxidase B Homo sapiens 80-85 30384046-3 2019 Linker length and flexibility played a key role in enhancing P-gp affinity as proved by the most potent P-gp modulator (3h, IC50 = 70 nM). Tritium 120-122 PGP Canis lupus familiaris 61-65 2744079-5 1989 The dissociation rate was markedly decreased (t1/2 = greater than 24h) at 0 degrees C. MAO-B, but not MAO-A inhibitors, effectively prevented the binding of [3H]Ro 19-6327. Tritium 158-160 monoamine oxidase B Homo sapiens 87-92 2744079-6 1989 Like [3H]Ro 16-6491, [3H]Ro 19-6327 is recognized as a substrate by MAO-B, being eventually deaminated by the enzyme. Tritium 6-8 monoamine oxidase B Homo sapiens 68-73 2744079-6 1989 Like [3H]Ro 16-6491, [3H]Ro 19-6327 is recognized as a substrate by MAO-B, being eventually deaminated by the enzyme. Tritium 22-24 monoamine oxidase B Homo sapiens 68-73 2744079-7 1989 Since the deaminated aldehyde derivative of Ro 19-6327 did not inhibit MAO-B, a still unidentified reversible adduct, formed at the MAO-B active site, might explain the high potency and selectivity of [3H]Ro 19-6327. Tritium 202-204 monoamine oxidase B Homo sapiens 132-137 2744079-10 1989 The presence of unlabelled MAO-B inhibitors in the incubation mixture prevented the covalent incorporation of [3H]Ro 19-6327. Tritium 111-113 monoamine oxidase B Homo sapiens 27-32 2744079-11 1989 The irreversible MAO-B inhibitor, [3H] pargyline, labelled a protein with a molecular weight identical to the protein labelled by [3H]Ro 19-6327. Tritium 35-37 monoamine oxidase B Homo sapiens 17-22 3117687-2 1987 Hen egg lysozyme-resistant, extensively O-acetylated [3H]PG (O-PG) from strain FA19 and lysozyme-sensitive, non-O-acetylated [14C]PG (non-O-PG) from strain RD5 (each containing label in both glucosamine and muramic acid) were mixed and incubated with granule extract at pHs 4.5, 5.5, and 6.5. Tritium 54-56 basic transcription factor 3 pseudogene 11 Homo sapiens 61-65 3668254-5 1987 GM3 lactone was detected in melanoma as 3H-labeled GM3 gangliosidol after melanoma cells were directly treated with NaB[3H]4. Tritium 40-42 granulocyte macrophage antigen 3 Mus musculus 0-3 3668254-5 1987 GM3 lactone was detected in melanoma as 3H-labeled GM3 gangliosidol after melanoma cells were directly treated with NaB[3H]4. Tritium 40-42 granulocyte macrophage antigen 3 Mus musculus 51-54 3668254-5 1987 GM3 lactone was detected in melanoma as 3H-labeled GM3 gangliosidol after melanoma cells were directly treated with NaB[3H]4. Tritium 120-122 granulocyte macrophage antigen 3 Mus musculus 0-3 3668254-5 1987 GM3 lactone was detected in melanoma as 3H-labeled GM3 gangliosidol after melanoma cells were directly treated with NaB[3H]4. Tritium 120-122 granulocyte macrophage antigen 3 Mus musculus 51-54 30384046-3 2019 Linker length and flexibility played a key role in enhancing P-gp affinity as proved by the most potent P-gp modulator (3h, IC50 = 70 nM). Tritium 120-122 PGP Canis lupus familiaris 104-108 3683774-0 1987 The chronic administration of dopamine antagonists and methamphetamine changed the [3H]-cholecystokinin-8 binding sites in the rat frontal cortex. Tritium 84-86 cholecystokinin Rattus norvegicus 88-103 3683774-1 1987 The specific cholecystokinin (CCK) binding in the slide-mounted tissue sections of the rat frontal cortex was measured with [3H]-CCK-8. Tritium 125-127 cholecystokinin Rattus norvegicus 13-28 3683774-1 1987 The specific cholecystokinin (CCK) binding in the slide-mounted tissue sections of the rat frontal cortex was measured with [3H]-CCK-8. Tritium 125-127 cholecystokinin Rattus norvegicus 30-33 3683774-1 1987 The specific cholecystokinin (CCK) binding in the slide-mounted tissue sections of the rat frontal cortex was measured with [3H]-CCK-8. Tritium 125-127 cholecystokinin Rattus norvegicus 129-132 3683774-3 1987 The chronic administration of dopamine (DA) antagonists and methamphetamine (MAP) showed a tendency to increase the [3H]-CCK-8 binding sites (Bmax) in the frontal cortex. Tritium 117-119 cholecystokinin Rattus norvegicus 121-124 2830975-0 1987 Localization of kappa opioid receptor binding sites in human forebrain using [3H]U69,593: comparison with [3H]bremazocine. Tritium 78-80 opioid receptor kappa 1 Homo sapiens 16-37 2830975-2 1987 The autoradiographic distribution of kappa opioid receptor binding sites in human brain was examined using two radiolabeled probes, namely [3H]U69,593 and [3H]bremazocine. Tritium 140-142 opioid receptor kappa 1 Homo sapiens 37-58 2830975-2 1987 The autoradiographic distribution of kappa opioid receptor binding sites in human brain was examined using two radiolabeled probes, namely [3H]U69,593 and [3H]bremazocine. Tritium 156-158 opioid receptor kappa 1 Homo sapiens 37-58 2496135-6 1989 Macrophage-produced [3H]PAF was resolved by HPLC, extracted, treated with phospholipase-C, and acetylated to facilitate quantitation of 1-O-alkyl-2-acetyl-GPC (PAF) from 1-O-acyl-2-acetyl-GPC (acylPAF). Tritium 21-23 patchy fur Mus musculus 24-27 2646474-3 1989 The anti-IL2 MoAb, termed 8H-10, was an IgG2a which inhibited IL2-driven [3H]TdR incorporation in cytolytic T lymphocyte line cells at a dilution of 2(6). Tritium 74-76 gamma-2a immunoglobulin heavy chain Rattus norvegicus 40-45 2784210-2 1989 rIL-7 alone stimulated some DNA synthesis (measured by [3H]thymidine uptake) but not actual growth (increase in cell number) of the pro-B clones. Tritium 56-58 interleukin 7 Rattus norvegicus 0-5 2494988-2 1989 The production of PAF is concentration- and time-dependent and, based upon [3H]serotonin release assays, approx. Tritium 76-78 PCNA clamp associated factor Rattus norvegicus 18-21 2830975-8 1987 This indicates that [3H]U69,593 is a highly selective ligand of the kappa opioid receptor type. Tritium 21-23 opioid receptor kappa 1 Homo sapiens 68-89 30290201-4 2018 Binding of [3H]nisoxetine to noradrenaline transporter (NET), [3H]GBR 12,935 to dopamine transporter (DAT) and [3H]citalopram to serotonin transporter (SERT) were analyzed by autoradiography. Tritium 12-14 solute carrier family 6 member 2 Rattus norvegicus 29-54 30290201-4 2018 Binding of [3H]nisoxetine to noradrenaline transporter (NET), [3H]GBR 12,935 to dopamine transporter (DAT) and [3H]citalopram to serotonin transporter (SERT) were analyzed by autoradiography. Tritium 12-14 solute carrier family 6 member 2 Rattus norvegicus 56-59 2891480-5 1987 The ratio of 35S:3H in the plasma after dosing rats with 100 mg/kg [35S]KS 1/4-[3H]DAVLB remained reasonably constant during 336 hr. Tritium 17-19 zinc finger protein 382 Rattus norvegicus 72-76 2537329-5 1989 The VDR of cultured skin fibroblasts of the affected children (G1 and G2) as well as the parents (G3 and G4) bound [3H]1,25-(OH)2D3 normally (Kd = 2-3 X 10(-11) mol/L; maximal number of binding sites = 20-40 fmol/mg protein). Tritium 116-118 vitamin D receptor Homo sapiens 4-7 30217571-5 2018 ATP-dependent uptake of [3H]-pravastatin into vesicles expressing MRP1-5, BCRP, and P-gp, as well as the results of inhibition studies suggest that BCRP and MRP1 are the major placental efflux transporters responsible for the in vitro uptake of pravastatin. Tritium 25-27 phosphoglycolate phosphatase Homo sapiens 84-88 2537329-5 1989 The VDR of cultured skin fibroblasts of the affected children (G1 and G2) as well as the parents (G3 and G4) bound [3H]1,25-(OH)2D3 normally (Kd = 2-3 X 10(-11) mol/L; maximal number of binding sites = 20-40 fmol/mg protein). Tritium 116-118 proline rich protein BstNI subfamily 3 Homo sapiens 63-72 2465355-7 1989 The uptake of [3H]retinol from RBP was inhibited by high concentrations of holo-RBP, with half maximal inhibition occurring at 3 microM holo-RBP. Tritium 15-17 retinol binding protein 4, plasma Mus musculus 31-34 2465355-7 1989 The uptake of [3H]retinol from RBP was inhibited by high concentrations of holo-RBP, with half maximal inhibition occurring at 3 microM holo-RBP. Tritium 15-17 retinol binding protein 4, plasma Mus musculus 80-83 2465355-7 1989 The uptake of [3H]retinol from RBP was inhibited by high concentrations of holo-RBP, with half maximal inhibition occurring at 3 microM holo-RBP. Tritium 15-17 retinol binding protein 4, plasma Mus musculus 80-83 2491852-11 1989 The Kd value of 4 microM for GTP was estimated (a) by the extent of quenching of tryptophan fluorescence of GEF in the presence of GTP and (b) by the binding of [3H]GTP to GEF as measured on nitrocellulose membranes. Tritium 162-164 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 108-111 3476498-4 1987 In the present studies, purified 3 alpha-HSD catalyzed rapid 3H loss from [3 beta-3H, C24-14C]lithocholic and chenodeoxycholic acids without net conversion to 3-oxo bile acids under physiologic pH and redox conditions. Tritium 61-63 aldo-keto reductase family 1, member C14 Rattus norvegicus 33-44 3476498-11 1987 3H loss from the 3 beta position therefore can be used to probe the interaction between bile acids and cytosolic 3 alpha-HSD in intact cells, and indomethacin is capable of inhibiting this interaction. Tritium 0-2 aldo-keto reductase family 1, member C14 Rattus norvegicus 113-124 3625281-5 1987 In membranes prepared from IPN, NTS, and AP, 3H-364,718 binding was of high affinity (Kd = 0.14 nM), saturable (Bmax = 20 fmol/mg protein), and inhibited by compounds previously shown to act at pancreatic CCK receptors. Tritium 45-47 cholecystokinin Rattus norvegicus 205-208 2491852-11 1989 The Kd value of 4 microM for GTP was estimated (a) by the extent of quenching of tryptophan fluorescence of GEF in the presence of GTP and (b) by the binding of [3H]GTP to GEF as measured on nitrocellulose membranes. Tritium 162-164 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 172-175 30090948-7 2018 IFNgamma decreased AQP3 mRNA expression in HT-29 cells in a time- and concentration-dependent manner and reduced functional AQP3 protein expression (decreased 3H-labelled glycerol uptake). Tritium 159-161 aquaporin 3 (Gill blood group) Homo sapiens 124-128 2782046-2 1989 Using [3H]-thymidine autoradiography, immunocytochemistry for microglial markers and electron microscopy, we could show that when ricin was administered together with the cytostatic drug adriamycin, the retrogradely transported adriamycin inhibits the macrophage response induced by toxic ricin. Tritium 7-9 ricin Ricinus communis 130-135 2441401-1 1987 Exposure of isolated rat gastric mucosal cells to 10(-10) and 10(-9) M gastrin (G-17-I) for 2 hr significantly stimulated [3H]leucine incorporation (15 min pulse) into protein by 100 and 212%, respectively, when compared with the basal levels. Tritium 123-125 gastrin Rattus norvegicus 71-78 29864448-8 2018 alpha7-containing and beta2-containing nAChRs were involved in nicotine-induced [3H]-GABA release in control and mdx synaptosomes. Tritium 81-83 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 0-6 3630545-3 1987 Relative binding affinities (RBA) for AR were first estimated in vitro with respect to [3H]testosterone for a series of structurally-related compounds. Tritium 88-90 androgen receptor Rattus norvegicus 38-40 2888864-1 1987 The interaction of the enantiomers of mianserin and propranolol with the binding of [3H]5-hydroxytryptamine ([3H]5-HT) to the 5-HT1A, 5-HT1B and 5-HT1C sites, and with the binding of [3H]ketanserin to the 5-HT2 site, has been evaluated in rat brain membranes. Tritium 85-87 5-hydroxytryptamine receptor 1B Rattus norvegicus 134-140 3498585-11 1987 It markedly inhibits C3a-induced 3H-serotonin release from platelets in vitro and similarly inhibits the C3a-induced extravasation of Evans blue into the skin in vivo. Tritium 33-35 complement C3 Homo sapiens 21-24 3649236-5 1987 Elastase activity, measured by the hydrolysis of [3H]elastin, was highest in aneurysmal aortic homogenates, 92.1 +/- 43.7 U/mg protein (n = 18), falling to 46.9 +/- 13.3 U/mg protein (n = 13) in severely stenosed atherosclerotic aortic homogenates and 35.5 +/- 11.9 U/mg (n = 6) in grossly normal aortic homogenates. Tritium 50-52 elastin Homo sapiens 53-60 3593803-4 1987 A comparative study of competition between the DNA under study and DNA-cellulose for the binding to highly purified (approximately 2000-fold) glucocorticoid-receptor complexes (GRC) of rat liver revealed that the highly methylated rat embryo liver DNA bind the activated [3H]triamcinolone-receptor complexes with a lower (approximately 1.8-2 times) efficiency than do the DNA from the livers of adult and aged rats, including the hydrocortisone-induced ones. Tritium 272-274 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 142-165 2472227-5 1989 The major anabolite of [3H]DHAC, [3H]DHACTP, peaked at 110.3 +/- 30.7 microM in CEM/O and at 96.3 +/- 41.9 microM in CEM/dCk(-) cells at 9 and 12 h, respectively. Tritium 24-26 sticky Drosophila melanogaster 121-124 2472227-5 1989 The major anabolite of [3H]DHAC, [3H]DHACTP, peaked at 110.3 +/- 30.7 microM in CEM/O and at 96.3 +/- 41.9 microM in CEM/dCk(-) cells at 9 and 12 h, respectively. Tritium 34-36 sticky Drosophila melanogaster 121-124 2776485-3 1989 In situ hybridization of high-resolution somatic and pachytene chromosomes using a 3H-labeled cDNA probe of the ADA gene localized the gene to 20q12----q13.11. Tritium 83-85 adenosine deaminase Homo sapiens 112-115 2913283-3 1989 Proglumide, but not scopolamine, hexamethonium and [D-Pro2,D-Trp7,9]substance P inhibited the release of [3H]ACh induced by CCK and gastrin. Tritium 106-108 cholecystokinin Cavia porcellus 124-127 2913283-6 1989 CCK, but not gastrin, produced the Ca++-dependent and tetrodotoxin-sensitive release of endogenous GABA and [3H]GABA from LM-MP preparations preloaded with [3H]GABA. Tritium 109-111 cholecystokinin Cavia porcellus 0-3 2913283-6 1989 CCK, but not gastrin, produced the Ca++-dependent and tetrodotoxin-sensitive release of endogenous GABA and [3H]GABA from LM-MP preparations preloaded with [3H]GABA. Tritium 157-159 cholecystokinin Cavia porcellus 0-3 2913283-7 1989 The release of [3H]GABA induced by CCK was antagonized by proglumide, but not by scopolamine, hexamethonium and [D-Pro2,D-Trp7,9]substance P. These results provide evidence that the GABAergic neuron is stimulated by CCK, but not by gastrin and stimulates the cholinergic neuron. Tritium 16-18 cholecystokinin Cavia porcellus 35-38 2913283-7 1989 The release of [3H]GABA induced by CCK was antagonized by proglumide, but not by scopolamine, hexamethonium and [D-Pro2,D-Trp7,9]substance P. These results provide evidence that the GABAergic neuron is stimulated by CCK, but not by gastrin and stimulates the cholinergic neuron. Tritium 16-18 cholecystokinin Cavia porcellus 216-219 2530410-4 1989 A comparison between the results of these binding studies and ganglioside-induced decrease of CD4 expression demonstrated that every aspect of [3H]-GM1 binding concurs with ganglioside modulation of CD4 expression. Tritium 144-146 Cd4 molecule Rattus norvegicus 94-97 2530410-4 1989 A comparison between the results of these binding studies and ganglioside-induced decrease of CD4 expression demonstrated that every aspect of [3H]-GM1 binding concurs with ganglioside modulation of CD4 expression. Tritium 144-146 Cd4 molecule Rattus norvegicus 199-202 3033846-1 1987 Changes in [3H]TCDD binding to unoccupied liver Ah receptor were examined following chronic or acute administration of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) to adult female Sprague-Dawley rats. Tritium 12-14 aryl hydrocarbon receptor Rattus norvegicus 48-59 29217174-6 2018 Studies of its mechanism of action, by means of its capacity to act via the GABAA receptor ([3H]-flunitrazepam binding assay); the 5-HT1A receptor ([3H]-8-OH-DPAT binding assay) and the voltage-gated sodium channels (either using the patch clamp technique in hNav 1.2 expressed in HEK293 cell line or using veratrine, in vivo) were attempted. Tritium 149-151 5-hydroxytryptamine receptor 1A Homo sapiens 131-146 3104329-8 1987 TRH caused a 2-fold increase in secretion of [3H]mannose-labeled TSH glycopeptides due almost exclusively to a specific increase in structures that bound to ConA-Sepharose and eluted with 10mM alpha-methylglucoside, corresponding to biantennary complex or unusual hybrid species. Tritium 46-48 thyrotropin releasing hormone Mus musculus 0-3 3104329-11 1987 Moreover, ConA-Sepharose chromatography of secreted [3H]glucosamine- and [3H]fucose-labeled TSH glycopeptides showed similar increases in ConA-Sepharose binding with TRH as noted with [3H]mannose labeling. Tritium 52-56 thyrotropin releasing hormone Mus musculus 166-169 3104329-11 1987 Moreover, ConA-Sepharose chromatography of secreted [3H]glucosamine- and [3H]fucose-labeled TSH glycopeptides showed similar increases in ConA-Sepharose binding with TRH as noted with [3H]mannose labeling. Tritium 73-77 thyrotropin releasing hormone Mus musculus 166-169 3104329-11 1987 Moreover, ConA-Sepharose chromatography of secreted [3H]glucosamine- and [3H]fucose-labeled TSH glycopeptides showed similar increases in ConA-Sepharose binding with TRH as noted with [3H]mannose labeling. Tritium 53-55 thyrotropin releasing hormone Mus musculus 166-169 2811600-2 1989 [3H]5-HT labeled both 5-HT1A and 5-HT1C recognition sites, defined by nanomolar affinity for 8-OH-DPAT and mesulergine respectively; however, these represented only a fraction of total specific [3H]5-HT binding. Tritium 1-3 5-hydroxytryptamine receptor 1A Oryctolagus cuniculus 22-28 2509821-2 1989 Gamma interferon associated with liposomes caused an inhibition of [3H]-thymidine uptake induced by macrophage colony-stimulating factor (CSF-1), and primed macrophages for subsequent induction of tumoricidal activity by bacterial lipopolysaccharide (LPS). Tritium 68-70 colony stimulating factor 1 (macrophage) Mus musculus 138-143 2645590-4 1989 Human skin extracts incubated with either [3H]retinol or [3H]retinoic acid and analyzed by PAGE is a novel technique for the study of cellular retinol-(CRBP) and retinoic acid-(CRABP) binding proteins; it allows one to more specifically analyse these binding proteins and differentiate them from RBP. Tritium 58-60 retinol binding protein 4 Homo sapiens 153-156 28289968-7 2017 RESULTS: In vitro binding studies demonstrated a saturable binding site for [3H]LRRK2-IN-1 in rat kidney, rat brain striatum and human brain striatum with K d of 26 +- 3 and 43 +- 8, 48 +- 2 nM, respectively. Tritium 77-79 leucine-rich repeat kinase 2 Rattus norvegicus 80-85 2469075-4 1989 Immunoprecipitation of androgen receptor fractions with polyclonal anti-phosphotyrosine antibody resulted in the recovery of [3H]-DHT binding activity from nuclear receptor fractions and partially purified cytosolic receptor fractions prepared from 20- to 24-hr castrated rats. Tritium 126-128 androgen receptor Rattus norvegicus 23-40 2436807-2 1987 Pretreatment of peptone-elicited macrophages 1 day with 300-1200 U/ml CSF-1 induced moderate killing and greatly stimulated lymphokine (LK)-induced killing of [3H]thymidine-labeled TU5 sarcoma cells to levels above that seen with fresh macrophages. Tritium 160-162 colony stimulating factor 1 (macrophage) Mus musculus 70-75 3830059-4 1987 We now show that this appearance of the glucocorticoid receptor is presumably due to removal of hypothalamic dopamine from the intermediate lobe cells, since in this study dopamine (or its potent agonist bromocriptine) inhibits expression of the glucocorticoid receptor in intermediate pituitary cells in primary culture, as demonstrated by [3H] dexamethasone binding and immunocytochemistry. Tritium 342-344 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 40-63 3830059-4 1987 We now show that this appearance of the glucocorticoid receptor is presumably due to removal of hypothalamic dopamine from the intermediate lobe cells, since in this study dopamine (or its potent agonist bromocriptine) inhibits expression of the glucocorticoid receptor in intermediate pituitary cells in primary culture, as demonstrated by [3H] dexamethasone binding and immunocytochemistry. Tritium 342-344 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 246-269 3031661-5 1987 Additional studies with muscle slices incubated with [3H]norepinephrine or [3H]choline showed that neuropeptide Y stimulated norepinephrine release from sympathetic nerves, which, in turn, inhibited the release of acetylcholine via alpha 2 receptors located on postganglionic nerves. Tritium 53-57 pro-neuropeptide Y Cavia porcellus 99-113 2521959-6 1989 Drug interactions with putative 5-HT1D binding sites in bovine caudate membranes correlated significantly with their affinities for human membrane recognition sites labeled by 3H-5-HT in the presence of 100 nM 8-OH-DPAT + 100 nM mesulergine. Tritium 176-178 5-hydroxytryptamine receptor 1D Homo sapiens 32-38 3198596-7 1988 The addition of progesterone, a known inhibitor of the acyl-CoA:retinol acyltransferase reaction, consistently increased the rate of retinyl ester formation when [3H]retinol was delivered bound to CRBP. Tritium 163-165 retinol binding protein 1 Rattus norvegicus 197-201 2907497-2 1988 [3H]Uridine pulse-chase experiments show that the inhibition of RNA metabolism by anti-IgM antibody treatment occurs in the nucleus at the mu-RNA processing level and results in low levels of mature mu-mRNA expression. Tritium 1-3 immunoglobulin heavy constant mu Mus musculus 87-90 28630284-9 2017 Nalidixic acid decreased equilibrium content of [3H]PAH, suggesting that it may possibly be exchanged by OAT1. Tritium 49-51 solute carrier family 22 member 6 Homo sapiens 105-109 3031059-2 1987 [3H]LTD4 binding to RBL-1 membrane receptors was stereoselective, specific, and saturable. Tritium 1-3 RB transcriptional corepressor like 1 Rattus norvegicus 20-25 28409274-5 2017 RESULTS: [3H]AZ1 showed improved specificity to FFAR1, with decreased off-target binding compared to [3H]AZ2 and [3H]TAK-875, while retaining high affinity in the nanomolar range. Tritium 10-12 free fatty acid receptor 1 Homo sapiens 48-53 3031059-3 1987 The binding affinity and maximum binding density of [3H]LTD4 to RBL-1 membrane receptors were 0.9 +/- 0.2 nM and 800 +/- 125 fmol/mg protein, respectively. Tritium 53-55 RB transcriptional corepressor like 1 Rattus norvegicus 64-69 2850768-6 1988 It was shown that under anaerobic conditions the ferredoxin reductase/NADPH system could reduce actinomycin D to produce a semiquinone-imine free radical (aN = 2.8 (2N); aH = 2.8 (3H)). Tritium 180-182 ferredoxin reductase Bos taurus 49-69 28501514-5 2017 This was attributed to the poor stability of HDAC11 which lost 40% activity in 3h at 37 C. By adding 0.2muM SAHA, HDAC11 activity was stabilized during the 3-h assay period. Tritium 79-81 histone deacetylase 11 Homo sapiens 45-51 2852061-4 1988 Using a computerized autoradiographic subtraction technique, we have examined the regional distribution of meptazinol-sensitive [3H][D-Ala2,MePhe4,Gly(ol)5]enkephalin (DAGO) binding and compared this with the distribution of mu 1 binding determined by competition with low [D-Ala2,D-Leu5]enkephalin (DADL) concentrations. Tritium 129-131 sodium voltage-gated channel alpha subunit 4 Rattus norvegicus 225-229 3141589-3 1988 Scatchard analyses of [3H]5-HT-labeled 5-HT1D sites in human prefrontal cortex produced a KD value of 4.2 nM and Bmax of 126 fmol/mg protein. Tritium 23-25 5-hydroxytryptamine receptor 1D Homo sapiens 39-45 2849421-9 1988 A double-reciprocal plot of initial velocity versus [3H]retinol-RBP concentration gave an apparent Km of 116 +/- 13 nM. Tritium 53-55 retinol binding protein 4 Homo sapiens 64-67 3566791-5 1987 When [3H]FNPA was photoirradiated with the purified MAO-B, a single radioactive band associated with MAO-B was observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Tritium 6-8 monoamine oxidase B Homo sapiens 52-57 3566791-5 1987 When [3H]FNPA was photoirradiated with the purified MAO-B, a single radioactive band associated with MAO-B was observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Tritium 6-8 monoamine oxidase B Homo sapiens 101-106 3566791-7 1987 Complete tryptic-chymotryptic digestion of [3H]FNPA-labeled MAO-B resulted in three radioactive peaks on Sephadex G-25 column chromatography. Tritium 44-46 monoamine oxidase B Homo sapiens 60-65 3566791-8 1987 With the same digestion and separation procedures, only one major radioactive peak was observed for the [3H]pargyline-labeled MAO-B, and its elution volume was different from that of [3H]FNPA-labeled peptides. Tritium 105-107 monoamine oxidase B Homo sapiens 126-131 3031556-4 1987 [3H]TCP binding was significantly reduced in strata pyramidalia of CA1/CA2, CA3 and subiculum of DAT hippocampal formation compared to that of control. Tritium 1-3 carbonic anhydrase 1 Homo sapiens 67-70 2849421-10 1988 Transthyretin decreased the rate of uptake of [3H]retinol from RBP without substantially altering the steady-state uptake levels, suggesting that membranes take up retinol from uncomplexed RBP. Tritium 47-49 transthyretin Homo sapiens 0-13 2849421-10 1988 Transthyretin decreased the rate of uptake of [3H]retinol from RBP without substantially altering the steady-state uptake levels, suggesting that membranes take up retinol from uncomplexed RBP. Tritium 47-49 retinol binding protein 4 Homo sapiens 63-66 3031556-4 1987 [3H]TCP binding was significantly reduced in strata pyramidalia of CA1/CA2, CA3 and subiculum of DAT hippocampal formation compared to that of control. Tritium 1-3 carbonic anhydrase 2 Homo sapiens 71-74 28501514-5 2017 This was attributed to the poor stability of HDAC11 which lost 40% activity in 3h at 37 C. By adding 0.2muM SAHA, HDAC11 activity was stabilized during the 3-h assay period. Tritium 79-81 histone deacetylase 11 Homo sapiens 114-120 28374768-0 2017 Cortical laminar tau deposits and activated astrocytes in Alzheimer"s disease visualised by 3H-THK5117 and 3H-deprenyl autoradiography. Tritium 92-94 microtubule associated protein tau Homo sapiens 17-20 3558493-5 1987 Pretreatment of cells with phorbol-12, 13-dibutyrate (PDBu) for 24 h depleted cellular protein kinase C activity and inhibited the ability of TPA to induce these effects suggesting a direct involvement of protein kinase C. Similarly the bombesin stimulation of 32Pi into PC and of [3H]choline and [3H]phosphocholine release was inhibited by PDBu pretreatment. Tritium 282-284 gastrin releasing peptide Homo sapiens 237-245 3558493-5 1987 Pretreatment of cells with phorbol-12, 13-dibutyrate (PDBu) for 24 h depleted cellular protein kinase C activity and inhibited the ability of TPA to induce these effects suggesting a direct involvement of protein kinase C. Similarly the bombesin stimulation of 32Pi into PC and of [3H]choline and [3H]phosphocholine release was inhibited by PDBu pretreatment. Tritium 298-300 gastrin releasing peptide Homo sapiens 237-245 2881310-1 1987 Exposure of IEC-6 cells for 24 hr to either gastrin (50-500 ng/ml) or EGF (100-500 ng/ml) significantly stimulated (100-165%) the rate of [3H]thymidine incorporation into DNA (referred to as DNA synthesis) when compared with the corresponding basal levels. Tritium 139-141 gastrin Rattus norvegicus 44-51 3029065-1 1987 The characteristics of the Ah receptor from rat liver were investigated following the incubation of cytosol with [3H]2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) under various conditions, and using DEAE- and DNA-Sepharose chromatography and sucrose density gradient centrifugation. Tritium 114-116 aryl hydrocarbon receptor Rattus norvegicus 27-38 3474107-4 1987 We have developed a combined in situ hybridization procedure using biotinylated human DNA plus antibodies and 3H-labeled HRAS1 DNA plus autoradiography to locate the actively transforming oncogene within the human transgenome in a selection of these transformants. Tritium 110-112 Ras association domain family member 7 Homo sapiens 121-126 3178823-6 1988 [3H]Alkylmethoxy-GPC is poorly metabolized by both cell populations as is evident by the trace quantities of labeled metabolites formed; also, alkylmethoxyglycerols do not exert any cytotoxic activity toward undifferentiated cells. Tritium 1-3 glycophorin C (Gerbich blood group) Homo sapiens 17-20 3263052-3 1988 EGF stimulated [3H]thymidine uptake in confluent quiescent monolayers in a dose-dependent fashion, half-maximal stimulation occurring at 7.5 X 10(-11) M. Likewise, EGF-mediated cellular proliferation was dose dependent (50% effective dose = 5 X 10(-11) M) under reduced serum concentrations. Tritium 16-18 EGF Equus caballus 0-3 3263052-3 1988 EGF stimulated [3H]thymidine uptake in confluent quiescent monolayers in a dose-dependent fashion, half-maximal stimulation occurring at 7.5 X 10(-11) M. Likewise, EGF-mediated cellular proliferation was dose dependent (50% effective dose = 5 X 10(-11) M) under reduced serum concentrations. Tritium 16-18 EGF Equus caballus 164-167 2902801-1 1988 Prolactin (PRL) stimulates an increased rate of incorporation of [14C]acetate and [3H]glucose into lipids in cultured mammary gland explants from 10- to 14-day-pregnant mice. Tritium 83-85 prolactin Mus musculus 0-9 2839528-3 1988 Gerbils exhibited a high concentration of [3H]naloxone binding sites in the sr. pyramidale of the CA3 subfield, as observed in the rat. Tritium 42-45 carbonic anhydrase 3 Rattus norvegicus 98-101 28374768-0 2017 Cortical laminar tau deposits and activated astrocytes in Alzheimer"s disease visualised by 3H-THK5117 and 3H-deprenyl autoradiography. Tritium 107-109 microtubule associated protein tau Homo sapiens 17-20 28374768-2 2017 We here aimed to visualize in vitro the distribution of tau deposits and activated astrocytes across the cortical layers in autopsy AD brain tissue using the radiotracers 3H-THK5117 and 3H-deprenyl. Tritium 171-173 microtubule associated protein tau Homo sapiens 56-59 3119503-8 1987 When (3H-)PAF was incubated with the supernatant fraction of the pouch fluid it was metabolized into lyso-PAF time-dependently. Tritium 6-8 PCNA clamp associated factor Rattus norvegicus 10-13 28374768-2 2017 We here aimed to visualize in vitro the distribution of tau deposits and activated astrocytes across the cortical layers in autopsy AD brain tissue using the radiotracers 3H-THK5117 and 3H-deprenyl. Tritium 186-188 microtubule associated protein tau Homo sapiens 56-59 3119503-8 1987 When (3H-)PAF was incubated with the supernatant fraction of the pouch fluid it was metabolized into lyso-PAF time-dependently. Tritium 6-8 PCNA clamp associated factor Rattus norvegicus 106-109 2840461-5 1988 The early rise in ODC activity is followed by a protracted increase in enzyme activity, apparent protein measured by [3H]difluoromethylornithine binding, and by accumulation of steady state ODC mRNA. Tritium 118-121 ornithine decarboxylase, structural 1 Mus musculus 18-21 28374768-8 2017 The difference in regional binding patterns of 3H-THK5117 and AT8 antibody staining suggest additional tau binding sites detectable by 3H-THK5117. Tritium 47-49 microtubule associated protein tau Homo sapiens 103-106 28374768-8 2017 The difference in regional binding patterns of 3H-THK5117 and AT8 antibody staining suggest additional tau binding sites detectable by 3H-THK5117. Tritium 135-137 microtubule associated protein tau Homo sapiens 103-106 27798799-7 2017 The M + T + S mixture was a strong inhibitor of dehydrogenases, to a less degree catalase, urease, beta-glucosidase, and arylsulfatase, while being a weak inhibitor of phosphatases. Tritium 8-13 beta-glucosidase Zea mays 99-134 3392021-9 1988 A 3H-labeled single component was purified from the digests by sequential chromatography through anti-ALP-IgG-Sepharose, concanavalin A-Sepharose, Bio-Gel P-6, and TSK G-2000 columns. Tritium 2-4 tsukushi, small leucine rich proteoglycan Homo sapiens 164-167 3416830-4 1988 In order to define the substructure of the tubulin domain for MAP interaction, we have examined the binding of 3H-acetylated C-terminal peptides to MAP-2 and tau. Tritium 111-113 microtubule associated protein 2 Homo sapiens 148-153 3220834-1 1988 Ganglioside GM1(NeuAc), labeled at the C-3 position of sphingosine with tritium, was injected into C3H/He, C57BL/10, B10.AQR mice intraperitoneally. Tritium 72-79 coenzyme Q10A Mus musculus 12-22 3220834-9 1988 Most of the administered [3H]GM1(NeuAc)-NMe was incorporated in the liver, and was metabolized to GM3(NeuAc)-NMe, via GM2(NeuAc)-NMe, within 24 h. GM3(NeuAc)-NMe was the only radioactive compound in the subsequent 10 weeks, but disappeared from the liver gradually. Tritium 26-28 granulocyte macrophage antigen 3 Mus musculus 98-101 3220834-9 1988 Most of the administered [3H]GM1(NeuAc)-NMe was incorporated in the liver, and was metabolized to GM3(NeuAc)-NMe, via GM2(NeuAc)-NMe, within 24 h. GM3(NeuAc)-NMe was the only radioactive compound in the subsequent 10 weeks, but disappeared from the liver gradually. Tritium 26-28 granulocyte macrophage antigen 3 Mus musculus 147-150 20501143-1 1987 Specific binding sites for thyrotropin-releasing hormone (TRH) were labelled with the potent analog, [(3)H] (3-Me-His(2))TRH, in the brain and spinal cord of the mutant mouse spastic and nonafflicted littermate controls. Tritium 101-106 thyrotropin releasing hormone Mus musculus 27-56 20501143-1 1987 Specific binding sites for thyrotropin-releasing hormone (TRH) were labelled with the potent analog, [(3)H] (3-Me-His(2))TRH, in the brain and spinal cord of the mutant mouse spastic and nonafflicted littermate controls. Tritium 101-106 thyrotropin releasing hormone Mus musculus 58-61 20501143-1 1987 Specific binding sites for thyrotropin-releasing hormone (TRH) were labelled with the potent analog, [(3)H] (3-Me-His(2))TRH, in the brain and spinal cord of the mutant mouse spastic and nonafflicted littermate controls. Tritium 101-106 thyrotropin releasing hormone Mus musculus 121-124 3775385-3 1986 When pituitary sections were hybridized with this 3H-labeled POMC intron A probe, silver grains were predominantly localized over the nuclei of cells that expressed POMC in the anterior and intermediate lobes. Tritium 50-52 proopiomelanocortin Rattus norvegicus 61-65 3775385-3 1986 When pituitary sections were hybridized with this 3H-labeled POMC intron A probe, silver grains were predominantly localized over the nuclei of cells that expressed POMC in the anterior and intermediate lobes. Tritium 50-52 proopiomelanocortin Rattus norvegicus 165-169 27543355-9 2016 [3H]-GlySar and [3H]-l-His uptake receded to approximately 30% in the presence of His-Leu-LPV supporting the PepT1/PHT1 mediated uptake process. Tritium 1-3 solute carrier family 15 member 4 Homo sapiens 115-119 3815319-3 1986 Two galactoproteins, designated GPC-130 and GPC-80 (apparent molecular weight X 10(-3)) were labelled by galactose oxidase/NaB [3H]4 but in all four lines only GPC-130 was prominent. Tritium 128-130 glycophorin C (Gerbich blood group) Homo sapiens 32-35 3815319-3 1986 Two galactoproteins, designated GPC-130 and GPC-80 (apparent molecular weight X 10(-3)) were labelled by galactose oxidase/NaB [3H]4 but in all four lines only GPC-130 was prominent. Tritium 128-130 glycophorin C (Gerbich blood group) Homo sapiens 44-47 3815319-3 1986 Two galactoproteins, designated GPC-130 and GPC-80 (apparent molecular weight X 10(-3)) were labelled by galactose oxidase/NaB [3H]4 but in all four lines only GPC-130 was prominent. Tritium 128-130 glycophorin C (Gerbich blood group) Homo sapiens 44-47 3582266-4 1986 3H-oxytocin in human milk was stable even after incubation at 37 degrees C for 2 hours. Tritium 0-2 oxytocin/neurophysin I prepropeptide Homo sapiens 3-11 3026563-4 1986 The binding of [3H]somatostatin in both the frontal cortex and cerebellar cortex demonstrated pharmacological specificity, since somatostatin-28, but not somatostatin-28(1-12) or Des AA1,2,4,5,12,13, D-Trp8-somatostatin, competed for the binding sites. Tritium 16-18 somatostatin Homo sapiens 129-144 2967176-6 1988 By subcellular fractionation studies [3H]GEMSA-binding sites are localized to the granule fraction with ANF immunoreactivity. Tritium 38-40 natriuretic peptide A Rattus norvegicus 104-107 3360809-1 1988 Purified rat liver glucocorticoid receptor was covalently charged with [3H]glucocorticoid by photoaffinity labeling (UV irradiation of [3H]triamcinolone acetonide-glucocorticoid receptor) or affinity labeling (incubation with [3H]dexamethasone mesylate). Tritium 72-74 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 19-42 3360809-1 1988 Purified rat liver glucocorticoid receptor was covalently charged with [3H]glucocorticoid by photoaffinity labeling (UV irradiation of [3H]triamcinolone acetonide-glucocorticoid receptor) or affinity labeling (incubation with [3H]dexamethasone mesylate). Tritium 72-74 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 163-186 3390432-4 1988 Inactivation of [3H]pyridoxal 5"-phosphate ([3H]PLP) reconstituted GABA aminotransferase by 1 followed by denaturation releases [3H]PMP with no radioactivity remaining attached to the protein. Tritium 17-19 4-aminobutyrate aminotransferase Sus scrofa 67-88 3165067-0 1988 Labelling and exploration of the active site of enkephalinase (EC 3.4.24.11) in kidney membranes with [3H]thiorphan as ligand. Tritium 103-105 membrane metallo-endopeptidase Rattus norvegicus 48-61 3024779-6 1986 Bovine pineal PBR characterized with [3H]PK 11195 revealed a high density (relative to CBR) of high affinity binding sites (Kd = 1.08 +/- 0.30, Bmax = 776 +/- 33.0 fmol/mg protein). Tritium 38-40 translocator protein Bos taurus 14-17 3370479-2 1988 Kainic acid injection reduced [3H]cyclohexyladenosine binding in the CA3 region by 40% and in the CA1 region by 30%. Tritium 31-33 carbonic anhydrase 3 Rattus norvegicus 69-72 27543355-9 2016 [3H]-GlySar and [3H]-l-His uptake receded to approximately 30% in the presence of His-Leu-LPV supporting the PepT1/PHT1 mediated uptake process. Tritium 1-4 solute carrier family 15 member 4 Homo sapiens 115-119 3024779-8 1986 These observations are in sharp contrast to the rat pineal gland, in which both [3H]Ro 5-4864 and [3H]PK 11195 bind to a large number of PBR with high affinity (Kd approximately equal to 1.9 nM, Bmax approximately equal to 26 pmol/mg protein). Tritium 81-83 translocator protein Rattus norvegicus 137-140 3024779-8 1986 These observations are in sharp contrast to the rat pineal gland, in which both [3H]Ro 5-4864 and [3H]PK 11195 bind to a large number of PBR with high affinity (Kd approximately equal to 1.9 nM, Bmax approximately equal to 26 pmol/mg protein). Tritium 99-101 translocator protein Rattus norvegicus 137-140 27458015-6 2016 Furthermore, CCL2 inhibited [(3)H]cholesterol efflux to HDL/apoA1 in ECs. Tritium 30-33 C-C motif chemokine ligand 2 Homo sapiens 13-17 3530331-4 1986 The polypeptides with molecular weight of 130 000 and 90 000 were detected by SDS-polyacrylamide gel electrophoresis of DNA ligase I-[3H]AMP complex. Tritium 134-136 DNA ligase 1 Bos taurus 120-132 2900473-3 1988 Adrenaline (in the presence of rauwolscine), isoprenaline and the preferential beta 2-adrenoceptor agonist procaterol concentration-dependently increased the electrically evoked tritium overflow. Tritium 178-185 adrenoceptor beta 2 Homo sapiens 79-98 2830908-4 1988 Prolactin significantly stimulated the accumulation of 3H label in inositol monophosphate (IP1), inositol bisphosphate (IP2) and inositol trisphosphate (IP3) 1-3 h after addition of prolactin. Tritium 55-57 prolactin Mus musculus 0-9 27300692-6 2016 Similarly, inhibiting BCRP increased [3H]benzylpenicillin uptake in MDCKII-BCRP cells, while inhibiting P-gp in MDCKII-MDR cells had no effect on uptake although there was evidence that benzylpenicillin is a substrate for canine P-gp. Tritium 38-40 ATP binding cassette subfamily G member 2 Canis lupus familiaris 22-26 3276960-5 1988 When incubated with enkephalinase purified from renal brush border or with solubilized brush border proteins in the presence of antimouse IgG, the mAb specifically precipitates active enkephalinase as assessed by thiorphan inhibitable hydrolysis of [3H]-(D.Ala2, Leu5)]enkephalin. Tritium 250-252 membrane metallo-endopeptidase Rattus norvegicus 184-197 3026629-6 1986 The Kd determined by kinetic analysis of [3H]prazosin binding was calculated from the ratio K-1/K1 that gave a value of 3.05 nM, which generally agreed with the 1.83 nM determined by saturation experiments (Scatchard plot). Tritium 42-44 keratin 1 Rattus norvegicus 92-98 3525564-9 1986 Studies on its specificity revealed that neurotensin-(9-13) was the shortest neurotensin partial sequence that was able to fully inhibit [3H]neurotensin degradation. Tritium 138-140 neurotensin Rattus norvegicus 41-52 27300692-6 2016 Similarly, inhibiting BCRP increased [3H]benzylpenicillin uptake in MDCKII-BCRP cells, while inhibiting P-gp in MDCKII-MDR cells had no effect on uptake although there was evidence that benzylpenicillin is a substrate for canine P-gp. Tritium 38-40 ATP binding cassette subfamily G member 2 Canis lupus familiaris 75-79 3525564-9 1986 Studies on its specificity revealed that neurotensin-(9-13) was the shortest neurotensin partial sequence that was able to fully inhibit [3H]neurotensin degradation. Tritium 138-140 neurotensin Rattus norvegicus 77-88 25844889-3 2015 [3H]-Digoxin, [3H]-vinblastine and [3H]-abacavir were selected as model substrates for P-gp, MRP2, and BCRP. Tritium 0-5 PGP Canis lupus familiaris 87-91 3525564-9 1986 Studies on its specificity revealed that neurotensin-(9-13) was the shortest neurotensin partial sequence that was able to fully inhibit [3H]neurotensin degradation. Tritium 138-140 neurotensin Rattus norvegicus 77-88 2942638-12 1986 This subpopulation of total [3H]5-HT binding is designated 5-HT1B. Tritium 29-31 5-hydroxytryptamine receptor 1B Rattus norvegicus 59-65 3730374-5 1986 When the preparation is photolabelled with [3H]cytochalasin B prior to the separation only P0 and P1 are labelled. Tritium 44-46 crystallin gamma F, pseudogene Homo sapiens 91-100 2853593-2 1988 The extent of phosphorylation is dose-dependent and correlates with the mitogenic effect induced by bombesin, measured by [3H]thymidine incorporation. Tritium 123-125 gastrin releasing peptide Homo sapiens 100-108 2449905-7 1988 3H-labelled platelet membrane glycoproteins with apparent molecular weights of 130k, 25k, and 21k (under reduced conditions) corresponding to GPIb alpha, GPIb beta, and GPIX were immunoprecipitated by anti-PlE1 plasma. Tritium 0-2 glycoprotein Ib platelet subunit alpha Homo sapiens 142-152 2449905-7 1988 3H-labelled platelet membrane glycoproteins with apparent molecular weights of 130k, 25k, and 21k (under reduced conditions) corresponding to GPIb alpha, GPIb beta, and GPIX were immunoprecipitated by anti-PlE1 plasma. Tritium 0-2 glycoprotein Ib platelet subunit beta Homo sapiens 154-163 2856186-3 1988 Binding of [3H]LTB4 to the binding sites was inhibited by 50% with 0.31 ng LTB4. Tritium 12-14 prostaglandin reductase 1 Cavia porcellus 15-19 2856186-3 1988 Binding of [3H]LTB4 to the binding sites was inhibited by 50% with 0.31 ng LTB4. Tritium 12-14 prostaglandin reductase 1 Cavia porcellus 75-79 3263342-8 1988 The residual 3H-thymidine incorporation observed in the presence of anti-IL-2 and anti-IL-2R may represent cycling B-cells induced to proliferate by activated, but non-cyclin, T-cells. Tritium 13-15 interleukin 2 receptor, alpha chain Mus musculus 87-92 2836680-1 1988 To establish a simple and sensitive quantitation of leukotriene B4 (LTB4), we developed a radioreceptor assay (RRA) using a highly specific [3H]leukotriene B4[( 3H]LTB4) binding to a guinea pig spleen homogenate. Tritium 141-144 prostaglandin reductase 1 Cavia porcellus 68-72 3015138-2 1986 Both sites exhibit different sensitivity to sodium ions and therefore can be selectively investigated at [3H]BDNL-CCK7 concentration lower than 1 nM for the A site in Tris buffer and in Krebs buffer for the B site. Tritium 106-108 cholecystokinin Cavia porcellus 114-117 3488063-1 1986 Binding of androgens including 5 alpha-dihydrotestosterone, testosterone and androstenedione stabilized the androgen receptor from rat submandibular gland with respect to its [3H]methyltrienolone binding activity. Tritium 176-178 androgen receptor Rattus norvegicus 108-125 3958711-6 1986 These results suggest the presence of three binding sites for [3H]5-HT, one blocked by low concentrations of spiperone (5-HT1A), one blocked by low concentrations of mianserin (5-HT1C), and one blocked only by high concentrations of both mianserin and spiperone (5-HT1B). Tritium 63-65 5-hydroxytryptamine receptor 2C Rattus norvegicus 177-183 2836680-3 1988 Fifty percent inhibition of bound [3H]LTB4 was obtained by 2.5 nM of unlabeled LTB4. Tritium 35-37 prostaglandin reductase 1 Cavia porcellus 38-42 25844889-3 2015 [3H]-Digoxin, [3H]-vinblastine and [3H]-abacavir were selected as model substrates for P-gp, MRP2, and BCRP. Tritium 0-5 ATP binding cassette subfamily G member 2 Canis lupus familiaris 103-107 2836680-3 1988 Fifty percent inhibition of bound [3H]LTB4 was obtained by 2.5 nM of unlabeled LTB4. Tritium 35-37 prostaglandin reductase 1 Cavia porcellus 79-83 3958711-6 1986 These results suggest the presence of three binding sites for [3H]5-HT, one blocked by low concentrations of spiperone (5-HT1A), one blocked by low concentrations of mianserin (5-HT1C), and one blocked only by high concentrations of both mianserin and spiperone (5-HT1B). Tritium 63-65 5-hydroxytryptamine receptor 1B Rattus norvegicus 263-269 25385314-3 2015 In this work we demonstrate that [(3)H]L-tyrosine is primarily transported by the system L transporter LAT1 in human LN229 glioblastoma cells. Tritium 33-38 solute carrier family 7 member 5 Homo sapiens 103-107 3005314-6 1986 In conditions of high ionic strength the Ah receptor from rat hepatic cytosol dissociated to a [3H]TCDD-binding subunit which sedimented at 5.6 +/- 0.58 S, had a Stokes radius of 5.2 +/- 0.24 nm, and an apparent Mr of 121,000 +/- 5,600. Tritium 96-98 aryl hydrocarbon receptor Rattus norvegicus 41-52 3005314-8 1986 Whereas the Ah receptor from rat liver readily dissociated into a [3H]TCDD-binding subunit during brief exposure to 0.4 M KCl, the mouse Ah receptor resisted dissociation. Tritium 67-69 aryl hydrocarbon receptor Rattus norvegicus 12-23 3427414-1 1987 In the presence of the reuptake blocker, nomifensine (1 microM), neurotensin caused a dose-dependent increase in the 25 mM K+-evoked release of [3H]dopamine ([3H]-DA) from rat nucleus accumbens slices. Tritium 145-147 neurotensin Rattus norvegicus 65-76 3427414-4 1987 Superfusion of the DMI-treated rat tissue with buffer-containing neurotensin (100 nM) resulted in an increase in the K+-evoked release of [3H]DA to a level which was higher than that observed with tissue obtained from untreated rats. Tritium 139-141 neurotensin Rattus norvegicus 65-76 3485449-7 1986 Competition studies involving a range of several radioinert steroids revealed that the binding of [3H]-R1881 to cytosol (ARc) and nuclear extract was specific for androgen receptor when triamcinolone acetonide (10 microM) was added. Tritium 99-101 androgen receptor Rattus norvegicus 163-180 25547246-3 2015 METHODS: We performed a dose-time-dependent study with physiological concentrations of leptin: 5, 10 and 50 ng/ml, for 0, 30", 3h, 6h, 12h and 24h, also monitoring time-course changes in non-treated cells. Tritium 127-129 leptin Mus musculus 87-93 3948773-3 1986 Glucocorticoid receptor concentration, measured using [3H]dexamethasone as ligand, increased in all eight tissues, but the magnitude of this increase varied 30-fold among tissues. Tritium 55-57 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 0-23 3444378-2 1987 Metabolic studies in both Raji and L1210 leukemic cells on OM-GPC, 3H-labeled in the methyl groups of the choline moiety, showed a (diacyl)-phosphatidylcholine as the only labeled metabolite. Tritium 67-69 glycophorin C (Gerbich blood group) Homo sapiens 62-65 25547246-6 2015 In the short-term (30" and/or 3h), leptin significantly induced the expression of PGC1alpha, muscle carnitine palmitoyl transferase 1 (mCPT1), uncoupling protein 3 (UCP3), OB-Rb, Insulin receptor (InsR) and interleukins 6 and 15 (IL6, IL15). Tritium 30-32 leptin Mus musculus 35-41 3444378-4 1987 One of these compounds, hexadecylphosphocholine (He-PC), which was 3H-labeled in the methyl-choline groups, showed a formation of labeled (diacyl)-phosphatidylcholine similar to that found with OM-GPC. Tritium 67-69 glycophorin C (Gerbich blood group) Homo sapiens 197-200 25041479-10 2014 CONCLUSIONS AND IMPLICATIONS: Our results confirm previous findings that beta4-containing nACh receptors are critical for [(3) H]-ACh release in the mouse IPN. Tritium 122-128 basic helix-loop-helix family, member e23 Mus musculus 73-78 3119544-6 1987 PRL alone caused a modest increase in the number of 3H-thymidine-labeled nuclei in both tissues. Tritium 52-54 prolactin Mus musculus 0-3 2957378-6 1987 IGF-I was more potent than IGF-II in stimulating N-methyl-alpha-[14C]aminoisobutyric acid uptake, 2-[3H]deoxyglucose uptake, and [3H]leucine incorporation into cellular proteins. Tritium 101-103 insulin-like growth factor 2 Rattus norvegicus 27-33 3526299-2 1986 The degree of degradation of the peptide was determined by reverse phase HPLC separation of 3H-labelled neurotensin from 3H-labelled products. Tritium 92-94 neurotensin Rattus norvegicus 104-115 3526299-4 1986 In the absence of inhibitors, 20 to 50% of 3H-neurotensin was degraded giving mainly 3H-Tyr along with other unidentified 3H-labelled products. Tritium 43-45 neurotensin Rattus norvegicus 46-57 3526299-4 1986 In the absence of inhibitors, 20 to 50% of 3H-neurotensin was degraded giving mainly 3H-Tyr along with other unidentified 3H-labelled products. Tritium 85-87 neurotensin Rattus norvegicus 46-57 3526299-7 1986 In contrast, dynorphin(1-13), an inhibitor of a soluble, thiol dependent metallopeptidase which hydrolyses neurotensin at Arg8-Arg9, gave greater than 80% inhibition of 3H-neurotensin degradation in the slice preparation. Tritium 169-171 neurotensin Rattus norvegicus 107-118 3526299-7 1986 In contrast, dynorphin(1-13), an inhibitor of a soluble, thiol dependent metallopeptidase which hydrolyses neurotensin at Arg8-Arg9, gave greater than 80% inhibition of 3H-neurotensin degradation in the slice preparation. Tritium 169-171 neurotensin Rattus norvegicus 172-183 24953818-6 2014 Inhibition of nAChR-evoked [(3)H]-GABA release by (R)-baclofen was time sensitive and the effect was lost after prolonged exposure to the GABAB agonist. Tritium 28-32 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 14-19 3764405-1 1986 The glucocorticoid receptor (GCR) of the cytosol of liver and brain was studied by radioligand binding assay, using [3H] dexamethasone (Dex) as the ligand in lethally and nonlethally scalded rats. Tritium 117-119 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 29-32 3764405-4 1986 In order to determine whether the decrease of R0 was due to the translocation of [3H] Dex-GCR complex into the cell nuclei, the [3H] corticosterone (B) specific binding of the hepatic nuclei was measured by the exchange assay. Tritium 82-84 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 90-93 2869571-1 1986 The activity of lysyl oxidase, the cross-linking enzyme of elastin and collagen, was measured in culture media of human skin fibroblasts, human aortic medial smooth muscle cells (SMCs) and adventitial fibroblasts using [3H]lysine-labelled elastin substrate. Tritium 220-222 lysyl oxidase Homo sapiens 16-29 3611955-5 1987 In response to prolonged stimulation (120 minutes) with A23187, glomerular tissue synthesized PAF primarily via an acetyl transferase, utilizing either 3H-lyso-PAF or 3H-acetate/3H-acetyl-coenzyme A. Tritium 152-154 PCNA clamp associated factor Rattus norvegicus 94-97 3663641-1 1987 Effects of different transforming agents were examined on the sedimentation characteristics of calf uterine progesterone receptor (PR) bound to the synthetic progestin [3H]R5020 or the known progesterone antagonist [3H]RU38486 (RU486). Tritium 169-171 progesterone receptor Bos taurus 108-129 3607499-7 1987 Neuronal death in CA1 was preceded by the decrease in [3H]QNB binding activity in the stratum radiatum 1 and 2 days after ischemia. Tritium 55-57 carbonic anhydrase 1 Homo sapiens 18-21 3607499-8 1987 Marked decrease in [3H]QNB and [3H]CHA binding activity was noted in the CA1 subfield 3-27 days after recirculation. Tritium 20-22 carbonic anhydrase 1 Homo sapiens 73-76 3607499-8 1987 Marked decrease in [3H]QNB and [3H]CHA binding activity was noted in the CA1 subfield 3-27 days after recirculation. Tritium 32-34 carbonic anhydrase 1 Homo sapiens 73-76 3036249-6 1987 Compared to AGEPC, thrombin stimulated generation of [3H]IP2 and [3H]IP were severalfold higher. Tritium 54-56 prothrombin Oryctolagus cuniculus 19-27 3564084-6 1986 Sucrose density gradient (SDG) analysis of PgR showed that (3H)ORG2058-PgR complexes sediment as 8S, (3H)R5020-PgR complexes sediment as 8S and 4S, and (3H)DMNT-PgR complexes sediment as 8S entities along with dissociated (3H)steroid. Tritium 60-62 progesterone receptor Bos taurus 43-46 3036249-6 1987 Compared to AGEPC, thrombin stimulated generation of [3H]IP2 and [3H]IP were severalfold higher. Tritium 66-68 prothrombin Oryctolagus cuniculus 19-27 3564084-6 1986 Sucrose density gradient (SDG) analysis of PgR showed that (3H)ORG2058-PgR complexes sediment as 8S, (3H)R5020-PgR complexes sediment as 8S and 4S, and (3H)DMNT-PgR complexes sediment as 8S entities along with dissociated (3H)steroid. Tritium 60-62 progesterone receptor Bos taurus 71-74 25218897-5 2014 Moreover, 3h post-training administration of oroxylin A enhanced the mBDNF level at 9h after the acquisition trial compared to the level at 6h after the acquisition trial. Tritium 10-12 brain derived neurotrophic factor Mus musculus 69-74 3564084-6 1986 Sucrose density gradient (SDG) analysis of PgR showed that (3H)ORG2058-PgR complexes sediment as 8S, (3H)R5020-PgR complexes sediment as 8S and 4S, and (3H)DMNT-PgR complexes sediment as 8S entities along with dissociated (3H)steroid. Tritium 60-62 progesterone receptor Bos taurus 71-74 3564084-6 1986 Sucrose density gradient (SDG) analysis of PgR showed that (3H)ORG2058-PgR complexes sediment as 8S, (3H)R5020-PgR complexes sediment as 8S and 4S, and (3H)DMNT-PgR complexes sediment as 8S entities along with dissociated (3H)steroid. Tritium 60-62 progesterone receptor Bos taurus 71-74 3564084-6 1986 Sucrose density gradient (SDG) analysis of PgR showed that (3H)ORG2058-PgR complexes sediment as 8S, (3H)R5020-PgR complexes sediment as 8S and 4S, and (3H)DMNT-PgR complexes sediment as 8S entities along with dissociated (3H)steroid. Tritium 102-104 progesterone receptor Bos taurus 43-46 3564084-6 1986 Sucrose density gradient (SDG) analysis of PgR showed that (3H)ORG2058-PgR complexes sediment as 8S, (3H)R5020-PgR complexes sediment as 8S and 4S, and (3H)DMNT-PgR complexes sediment as 8S entities along with dissociated (3H)steroid. Tritium 102-104 progesterone receptor Bos taurus 71-74 3564084-6 1986 Sucrose density gradient (SDG) analysis of PgR showed that (3H)ORG2058-PgR complexes sediment as 8S, (3H)R5020-PgR complexes sediment as 8S and 4S, and (3H)DMNT-PgR complexes sediment as 8S entities along with dissociated (3H)steroid. Tritium 102-104 progesterone receptor Bos taurus 71-74 3564084-6 1986 Sucrose density gradient (SDG) analysis of PgR showed that (3H)ORG2058-PgR complexes sediment as 8S, (3H)R5020-PgR complexes sediment as 8S and 4S, and (3H)DMNT-PgR complexes sediment as 8S entities along with dissociated (3H)steroid. Tritium 102-104 progesterone receptor Bos taurus 71-74 3564084-6 1986 Sucrose density gradient (SDG) analysis of PgR showed that (3H)ORG2058-PgR complexes sediment as 8S, (3H)R5020-PgR complexes sediment as 8S and 4S, and (3H)DMNT-PgR complexes sediment as 8S entities along with dissociated (3H)steroid. Tritium 102-104 progesterone receptor Bos taurus 43-46 3496208-3 1987 Androgen receptor was measured by a previously validated in vitro binding assay using the synthetic androgen methyltrienolone [( 3H]R1881). Tritium 129-131 androgen receptor Rattus norvegicus 0-17 3496208-12 1987 The binding of [3H]R1881 was specific for AR and was not affected by the addition of radioinert progesterone to the incubation tube. Tritium 16-18 androgen receptor Rattus norvegicus 42-44 3564084-6 1986 Sucrose density gradient (SDG) analysis of PgR showed that (3H)ORG2058-PgR complexes sediment as 8S, (3H)R5020-PgR complexes sediment as 8S and 4S, and (3H)DMNT-PgR complexes sediment as 8S entities along with dissociated (3H)steroid. Tritium 102-104 progesterone receptor Bos taurus 71-74 24436229-2 2014 To investigate the mechanism(s) of this unusual DDI, first the effect of BUP, its circulating metabolites or their combination on [(3) H]-DIG transport by cells expressing human P-gp or human OATP4C1 was determined. Tritium 130-137 phosphoglycolate phosphatase Homo sapiens 178-182 3564084-6 1986 Sucrose density gradient (SDG) analysis of PgR showed that (3H)ORG2058-PgR complexes sediment as 8S, (3H)R5020-PgR complexes sediment as 8S and 4S, and (3H)DMNT-PgR complexes sediment as 8S entities along with dissociated (3H)steroid. Tritium 102-104 progesterone receptor Bos taurus 71-74 3564084-6 1986 Sucrose density gradient (SDG) analysis of PgR showed that (3H)ORG2058-PgR complexes sediment as 8S, (3H)R5020-PgR complexes sediment as 8S and 4S, and (3H)DMNT-PgR complexes sediment as 8S entities along with dissociated (3H)steroid. Tritium 102-104 progesterone receptor Bos taurus 71-74 3564084-6 1986 Sucrose density gradient (SDG) analysis of PgR showed that (3H)ORG2058-PgR complexes sediment as 8S, (3H)R5020-PgR complexes sediment as 8S and 4S, and (3H)DMNT-PgR complexes sediment as 8S entities along with dissociated (3H)steroid. Tritium 102-104 progesterone receptor Bos taurus 43-46 3564084-6 1986 Sucrose density gradient (SDG) analysis of PgR showed that (3H)ORG2058-PgR complexes sediment as 8S, (3H)R5020-PgR complexes sediment as 8S and 4S, and (3H)DMNT-PgR complexes sediment as 8S entities along with dissociated (3H)steroid. Tritium 102-104 progesterone receptor Bos taurus 71-74 3564084-6 1986 Sucrose density gradient (SDG) analysis of PgR showed that (3H)ORG2058-PgR complexes sediment as 8S, (3H)R5020-PgR complexes sediment as 8S and 4S, and (3H)DMNT-PgR complexes sediment as 8S entities along with dissociated (3H)steroid. Tritium 102-104 progesterone receptor Bos taurus 71-74 3564084-6 1986 Sucrose density gradient (SDG) analysis of PgR showed that (3H)ORG2058-PgR complexes sediment as 8S, (3H)R5020-PgR complexes sediment as 8S and 4S, and (3H)DMNT-PgR complexes sediment as 8S entities along with dissociated (3H)steroid. Tritium 102-104 progesterone receptor Bos taurus 71-74 3564084-7 1986 These data suggest that (3H)DMNT binds to PgR with lower affinity than (3H)ORG2058 and (3H)R5020. Tritium 25-27 progesterone receptor Bos taurus 42-45 3564086-5 1986 With [3H]-progesterone and [3H]R5020 as ligands the most efficient competitors also were R5020, progesterone and 5 alpha-DHP. Tritium 6-8 dihydropyrimidinase Rattus norvegicus 121-124 3564086-5 1986 With [3H]-progesterone and [3H]R5020 as ligands the most efficient competitors also were R5020, progesterone and 5 alpha-DHP. Tritium 28-30 dihydropyrimidinase Rattus norvegicus 121-124 3080414-6 1986 On a per DNA basis, the rate of [3H]serine incorporation into CS-PGs was 2.5 times the rate for DS-PG at 4 days before birth but decreased by 95% during the next 4 days. Tritium 33-35 decorin Rattus norvegicus 96-101 2440763-12 1987 Since previous work has shown that seizure mutants affect [3H]saxitoxin binding to the voltage-sensitive sodium channel, e(sei) may code for a gene product which interacts with this channel. Tritium 59-61 seizure Drosophila melanogaster 35-42 3040878-1 1987 Addition of platelet-activating factor (PAF) to cells doubly labeled with [14C]glycerol plus [3H]arachidonic acid resulted in a transient decrease of [14C]glycerol-labeled phosphatidylinositol (PI) and a transient increase of [14C]glycerol-labeled lysophosphatidylinositol (LPI). Tritium 94-97 PCNA clamp associated factor Rattus norvegicus 12-38 3040878-1 1987 Addition of platelet-activating factor (PAF) to cells doubly labeled with [14C]glycerol plus [3H]arachidonic acid resulted in a transient decrease of [14C]glycerol-labeled phosphatidylinositol (PI) and a transient increase of [14C]glycerol-labeled lysophosphatidylinositol (LPI). Tritium 94-97 PCNA clamp associated factor Rattus norvegicus 40-43 3038090-3 1987 The specific binding of [3H]NT was time dependent, reversible, and saturable. Tritium 25-27 neurotensin Rattus norvegicus 28-30 2888257-4 1987 It was shown that SST (10(-7)-10(-5) M) significantly inhibited the [3H]-thymidine incorporation. Tritium 69-71 somatostatin Homo sapiens 18-21 3593394-0 1987 Characterization of [3H](+/-)L-364,718 binding to solubilized cholecystokinin (CCK) receptors of rat pancreas. Tritium 21-23 cholecystokinin Rattus norvegicus 79-82 3593394-1 1987 The binding of [3H](+/-)L-364,718 (3S(-)-N-(2,3-dihydro-1-methyl-2-oxo-5-phenyl-1H-1,4-benzodiazepine-3-yl )-1H-indole-2-carboxamide), an extremely potent nonpeptide cholecystokinin (CCK) receptor antagonist, to digitonin-solubilized CCK receptors from rat pancreas was characterized. Tritium 16-18 cholecystokinin Rattus norvegicus 183-186 3593394-1 1987 The binding of [3H](+/-)L-364,718 (3S(-)-N-(2,3-dihydro-1-methyl-2-oxo-5-phenyl-1H-1,4-benzodiazepine-3-yl )-1H-indole-2-carboxamide), an extremely potent nonpeptide cholecystokinin (CCK) receptor antagonist, to digitonin-solubilized CCK receptors from rat pancreas was characterized. Tritium 16-18 cholecystokinin Rattus norvegicus 234-237 3593394-5 1987 Specific [3H](+/-)L-364,718 binding to solubilized CCK receptors was inhibited by both CCK receptor agonists and antagonists in a stereospecific manner. Tritium 10-12 cholecystokinin Rattus norvegicus 51-54 3593394-5 1987 Specific [3H](+/-)L-364,718 binding to solubilized CCK receptors was inhibited by both CCK receptor agonists and antagonists in a stereospecific manner. Tritium 10-12 cholecystokinin Rattus norvegicus 87-90 3001071-2 1986 We have investigated the subcellular localization of the peripheral-type benzodiazepine receptor in rat adrenal gland using the high affinity ligand 3H-labeled 1-(2-chlorophenyl)-N-methyl-(1-methylpropyl)-3-isoquinoline carboxamide ([3H]PK11195). Tritium 149-151 translocator protein Rattus norvegicus 57-96 3001071-2 1986 We have investigated the subcellular localization of the peripheral-type benzodiazepine receptor in rat adrenal gland using the high affinity ligand 3H-labeled 1-(2-chlorophenyl)-N-methyl-(1-methylpropyl)-3-isoquinoline carboxamide ([3H]PK11195). Tritium 234-236 translocator protein Rattus norvegicus 57-96 3001071-6 1986 Scatchard analysis and drug displacement studies of the binding of [3H] PK11195 to intact mitochondria and to the outer membrane-enriched digitonin extract further confirm the localization of the peripheral-type benzodiazepine receptor to the mitochondrial outer membrane. Tritium 68-70 translocator protein Rattus norvegicus 196-235 24789780-6 2014 Here, we show that similar to HIV-1 Gag lacking the p6 domain (GagDeltap6), RSV H6.Gag.3h is a more efficient chaperone on a molar basis than NC; however, in contrast to the HIV-1 system, both RSV H6.Gag.3h and NC have comparable annealing rates at protein saturation. Tritium 87-89 Pr76 polyprotein precursor Rous sarcoma virus 63-66 2445628-5 1987 The altered electrophoretic form of pp60c-src was also shown to be radiolabeled with [3H]myristate, indicating that pp60c-src is fatty-acylated in neurons, as is pp60c-src in fibroblasts. Tritium 86-88 SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus 36-45 3598396-7 1987 The addition of antiserum to hepatic lipase increased the amount of 3H found in 1,2(2,3)-diacylglycerols and inhibited the formation of free [3H]arachidonic acid. Tritium 68-70 lipase C, hepatic type Bos taurus 29-43 3472760-9 1986 Finally, by substitution of a homologous TPK for that of hIR, we find that although such a hybrid is capable of insulin-dependent transmembrane signaling (phosphorylation of the hybrid beta-subunit on tyrosine residues), the hybrid IR.ros molecule does not function as an IR in such cells: It mediates neither short-term (uptake of 2-deoxyglucose) nor long-term (incorporation of [3H]thymidine) effects of insulin (L. Ellis et al., in prep.). Tritium 381-383 potassium inwardly rectifying channel subfamily J member 4 Homo sapiens 57-60 24789780-6 2014 Here, we show that similar to HIV-1 Gag lacking the p6 domain (GagDeltap6), RSV H6.Gag.3h is a more efficient chaperone on a molar basis than NC; however, in contrast to the HIV-1 system, both RSV H6.Gag.3h and NC have comparable annealing rates at protein saturation. Tritium 87-89 Pr76 polyprotein precursor Rous sarcoma virus 63-66 3472760-9 1986 Finally, by substitution of a homologous TPK for that of hIR, we find that although such a hybrid is capable of insulin-dependent transmembrane signaling (phosphorylation of the hybrid beta-subunit on tyrosine residues), the hybrid IR.ros molecule does not function as an IR in such cells: It mediates neither short-term (uptake of 2-deoxyglucose) nor long-term (incorporation of [3H]thymidine) effects of insulin (L. Ellis et al., in prep.). Tritium 381-383 potassium inwardly rectifying channel subfamily J member 4 Homo sapiens 58-60 3598396-12 1987 Antiserum to hepatic lipase inhibited the postheparin plasma hydrolysis of phosphatidylethanolamine and 3H-labeled phosphatidylcholine by about 60%, but the 14C-labeled phosphatidylcholine by only 27%. Tritium 104-106 lipase C, hepatic type Bos taurus 13-27 24789780-7 2014 The NC domain of RSV H6.Gag.3h is required for annealing, whereas deletion of the matrix (MA) domain, which stimulates the rate of HIV-1 GagDeltap6 annealing, has little effect on RSV H6.Gag.3h chaperone function. Tritium 28-30 Pr76 polyprotein precursor Rous sarcoma virus 24-27 24680923-7 2014 Plasma EPO concentration was induced as early as 3h following acute anemic and hypoxemic hypoxia and suppressed by clinically relevant doses of isoflurane in a dose-dependent manner. Tritium 49-51 erythropoietin Mus musculus 7-10 2883654-7 1987 In NIH 3T3 cells carrying a glucocorticoid-inducible v-Ha-ras gene, a close correlation was found between the expression of p21ras and the loss of PDGF-stimulated [3H]InsP1 accumulation. Tritium 164-166 Harvey rat sarcoma virus oncogene Mus musculus 124-130 2820381-7 1987 Thrombin (2 units/ml) or GTP (4-100 microM) alone weakly stimulated [3H]IP2 formation, but together they acted synergistically to exert an effect comparable with that of 10 microM-GTP[S]. Tritium 69-71 prothrombin Oryctolagus cuniculus 0-8 2820381-10 1987 Incubation with either GTP[S] or thrombin and GTP decreased membrane [3H]phosphatidylinositol 4-phosphate ([H]PIP) and prevented an increase in [3H]phosphatidylinositol 4,5-bisphosphate ([3H]PIP2) observed in controls. Tritium 70-72 prothrombin Oryctolagus cuniculus 33-41 3029327-5 1987 Protein P55 was only partially degraded by trypsin, and whether it was labeled with [3H]flunitrazepam or [3H]Ro 15-4513 it yielded the same two proteolytic peptides with apparent molecular weights of 42,000 and 45,000. Tritium 85-87 MAGUK p55 scaffold protein 1 Rattus norvegicus 8-11 2422446-9 1986 Our results show that photoaffinity labelling of androgen receptors with [3H]R1881 as ligand can be applied for characterization of partial purified androgen receptor preparations. Tritium 74-76 androgen receptor Rattus norvegicus 49-66 3012081-9 1986 It is concluded that 3H-opioids bind to at least three sites--mu 1, mu 2 and delta. Tritium 21-23 sodium voltage-gated channel alpha subunit 4 Rattus norvegicus 62-66 3012081-9 1986 It is concluded that 3H-opioids bind to at least three sites--mu 1, mu 2 and delta. Tritium 21-23 adaptor related protein complex 2 subunit mu 1 Rattus norvegicus 68-82 4063532-6 1985 The assay for PAF acetylhydrolase was based on the release of 3H from PAF that was labelled in the acetate moiety of the sn-2 position. Tritium 62-64 phospholipase A2 group VII Homo sapiens 14-33 4089231-9 1985 Intraperitoneal injection of neurotensin induced an increase of pancreatic DNA content and stimulated [3H]thymidine incorporation into DNA (control: 11 000 dpm/g; neurotensin: 15 800 dpm/g pancreas). Tritium 103-105 neurotensin Rattus norvegicus 29-40 4052809-1 1985 Characteristics of angiotensin II (AII) binding have been determined in cultured mouse spinal cord neurones using [125I]AII and [3H]AII. Tritium 129-131 arginase type II Mus musculus 19-33 4052809-1 1985 Characteristics of angiotensin II (AII) binding have been determined in cultured mouse spinal cord neurones using [125I]AII and [3H]AII. Tritium 129-131 arginase type II Mus musculus 35-38 24286867-8 2014 Hsp90 inhibition during the first 3h in culture promotes multiple axon morphology, while this inhibition after 3h slows down axonal elongation. Tritium 34-36 heat shock protein 90 alpha family class A member 1 Homo sapiens 0-5 2865340-4 1985 [3H]NAAG specific binding was displaceable by serine-o-sulfate, quisqualate, ibotenate, and glutamate with K1"s in the nanomolar range, whereas the amino-phosphono analogues displaced [3H] NAAG in the micromolar range (APB greater than APV greater than APH). Tritium 1-3 acylaminoacyl-peptide hydrolase Rattus norvegicus 253-256 4052436-4 1985 The addition of unlabeled lyso-PAF to incubations initiated with [3H]PAF results in an increase in the amount of lyso-[3H]PAF product formed and a decrease in the final product, [3H]alkylacyl-GPC; however, the total amount of [3H]PAF metabolized remains unchanged. Tritium 66-68 PCNA clamp associated factor Rattus norvegicus 31-34 3026794-2 1987 We investigated the presence of specific binding sites for [3H]OT and [3H]arginine VP (AVP) in membranes prepared from tunica albuginea, epididymis, and vas deferens from prepubertal pigs 2-16 weeks of age. Tritium 60-62 oxytocin/neurophysin I prepropeptide Sus scrofa 63-65 3803315-0 1987 Histological evaluation of the dopaminergic regulation of proopiomelanocortin gene expression in the intermediate lobe of the rat pituitary, involving in situ hybridization and [3H]thymidine uptake measurement. Tritium 178-180 proopiomelanocortin Rattus norvegicus 58-77 24036158-3 2013 We studied the interaction of 12 immunosuppressants with OAT1- and OAT3-mediated [(3)H]-methotrexate (MTX) uptake in cells, and adenosine triphosphate-dependent [(3)H]-MTX transport in membrane vesicles isolated from human embryonic kidney 293 cells overexpressing human MRP2 and MRP4. Tritium 83-86 solute carrier family 22 member 8 Homo sapiens 67-71 3576519-4 1987 While there was no change in the saline control, thrombin induced a reduction in the content of [3H]alkylacyl-GPC, accompanied by an increase in [3H]lyso-PAF presumably by stimulation of phospholipase A2. Tritium 97-99 glycophorin C (Gerbich blood group) Homo sapiens 110-113 3028264-5 1987 Presence of bound [3H]TCDD substantially protected the Ah receptor against loss of ligand binding function when the cytosol was exposed to sulfhydryl-modifying reagents. Tritium 19-21 aryl hydrocarbon receptor Rattus norvegicus 55-66 4052436-4 1985 The addition of unlabeled lyso-PAF to incubations initiated with [3H]PAF results in an increase in the amount of lyso-[3H]PAF product formed and a decrease in the final product, [3H]alkylacyl-GPC; however, the total amount of [3H]PAF metabolized remains unchanged. Tritium 66-68 PCNA clamp associated factor Rattus norvegicus 69-72 4052436-4 1985 The addition of unlabeled lyso-PAF to incubations initiated with [3H]PAF results in an increase in the amount of lyso-[3H]PAF product formed and a decrease in the final product, [3H]alkylacyl-GPC; however, the total amount of [3H]PAF metabolized remains unchanged. Tritium 66-68 PCNA clamp associated factor Rattus norvegicus 69-72 4052436-4 1985 The addition of unlabeled lyso-PAF to incubations initiated with [3H]PAF results in an increase in the amount of lyso-[3H]PAF product formed and a decrease in the final product, [3H]alkylacyl-GPC; however, the total amount of [3H]PAF metabolized remains unchanged. Tritium 66-68 PCNA clamp associated factor Rattus norvegicus 69-72 4052436-4 1985 The addition of unlabeled lyso-PAF to incubations initiated with [3H]PAF results in an increase in the amount of lyso-[3H]PAF product formed and a decrease in the final product, [3H]alkylacyl-GPC; however, the total amount of [3H]PAF metabolized remains unchanged. Tritium 66-69 PCNA clamp associated factor Rattus norvegicus 31-34 4052436-4 1985 The addition of unlabeled lyso-PAF to incubations initiated with [3H]PAF results in an increase in the amount of lyso-[3H]PAF product formed and a decrease in the final product, [3H]alkylacyl-GPC; however, the total amount of [3H]PAF metabolized remains unchanged. Tritium 119-121 PCNA clamp associated factor Rattus norvegicus 31-34 4052436-4 1985 The addition of unlabeled lyso-PAF to incubations initiated with [3H]PAF results in an increase in the amount of lyso-[3H]PAF product formed and a decrease in the final product, [3H]alkylacyl-GPC; however, the total amount of [3H]PAF metabolized remains unchanged. Tritium 119-121 PCNA clamp associated factor Rattus norvegicus 69-72 4052436-4 1985 The addition of unlabeled lyso-PAF to incubations initiated with [3H]PAF results in an increase in the amount of lyso-[3H]PAF product formed and a decrease in the final product, [3H]alkylacyl-GPC; however, the total amount of [3H]PAF metabolized remains unchanged. Tritium 119-121 PCNA clamp associated factor Rattus norvegicus 69-72 4052436-4 1985 The addition of unlabeled lyso-PAF to incubations initiated with [3H]PAF results in an increase in the amount of lyso-[3H]PAF product formed and a decrease in the final product, [3H]alkylacyl-GPC; however, the total amount of [3H]PAF metabolized remains unchanged. Tritium 119-121 PCNA clamp associated factor Rattus norvegicus 69-72 4052436-5 1985 Unlabeled lyso-PAF thus enters the metabolic pool of the cell and competes with the deacetylated product of [3H]PAF, i.e., lyso-PAF, for acylation. Tritium 109-111 PCNA clamp associated factor Rattus norvegicus 15-18 4052436-5 1985 Unlabeled lyso-PAF thus enters the metabolic pool of the cell and competes with the deacetylated product of [3H]PAF, i.e., lyso-PAF, for acylation. Tritium 109-111 PCNA clamp associated factor Rattus norvegicus 112-115 4052436-5 1985 Unlabeled lyso-PAF thus enters the metabolic pool of the cell and competes with the deacetylated product of [3H]PAF, i.e., lyso-PAF, for acylation. Tritium 109-111 PCNA clamp associated factor Rattus norvegicus 112-115 2995366-4 1985 [3H]hTNF-beta was purified by high performance gel permeation chromatography and the major fraction was found to be monomeric by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Tritium 1-3 lymphotoxin alpha Homo sapiens 4-13 2995366-9 1985 Neutralizing polyclonal antibodies to hTNF-beta efficiently inhibited the binding of [3H]hTNF-beta to the cells. Tritium 86-88 lymphotoxin alpha Homo sapiens 38-47 2995366-9 1985 Neutralizing polyclonal antibodies to hTNF-beta efficiently inhibited the binding of [3H]hTNF-beta to the cells. Tritium 86-88 lymphotoxin alpha Homo sapiens 89-98 3025547-5 1987 Competition studies of the other peptides against 3H-DSLET and 3H[D-Ala2,MePhe4,Gly(ol)5]enkephalin (3H-DAMPGO) binding also implied high affinity binding of these peptides to mu1 sites. Tritium 50-52 sodium voltage-gated channel alpha subunit 4 Rattus norvegicus 176-179 3028841-11 1987 Additionally, these data suggest that the sites at which [3H]Ro 5-4864 and [3H]PK 11195 bind on the PBR may be overlapping, but not identical. Tritium 58-60 translocator protein Rattus norvegicus 100-103 3028841-11 1987 Additionally, these data suggest that the sites at which [3H]Ro 5-4864 and [3H]PK 11195 bind on the PBR may be overlapping, but not identical. Tritium 76-78 translocator protein Rattus norvegicus 100-103 3029638-3 1987 [3H]TCP binding in the CA1 region from Alzheimer"s patients was reduced an average of 40% while the other hippocampal regions were unaffected. Tritium 1-3 carbonic anhydrase 1 Homo sapiens 23-26 3029638-4 1987 It is proposed that the loss of [3H]TCP sites in the hippocampal CA1 region of certain Alzheimer"s cases is associated with the greater cell loss observed in cases of severe Alzheimer"s disease. Tritium 33-35 carbonic anhydrase 1 Homo sapiens 65-68 3794699-3 1987 Inhibitors and substrates of MAO-B inhibited binding of [3H]Ro 16-6491, whereas MAO-A blockers were much less potent. Tritium 57-59 monoamine oxidase B Homo sapiens 29-34 23920221-4 2013 In this study, inhibitory effects of 14 compounds on the OATP1B1-mediated uptake of the prototypical substrates [3H]estradiol-17beta-glucuronide (E2G), [3H]estrone-3-sulfate (E1S), and [3H]sulfobromophthalein (BSP) were studied in OATP1B1-transfected cells. Tritium 113-116 solute carrier organic anion transporter family member 1B1 Homo sapiens 57-64 3794699-6 1987 In conclusion, [3H]Ro 16-6491 binds selectively to MAO-B and represents an excellent new radioligand probe for studying the regional tissue distribution of this enzyme in normal and pathological conditions. Tritium 16-18 monoamine oxidase B Homo sapiens 51-56 3819510-6 1987 In the 3H-Act-D uptake experiment, NaUCC-1 did uptake a relatively larger amount of 3H-Act-D (p less than 0.01) and BeWo a smaller amount of it (p less than 0.05). Tritium 7-9 ACTD Homo sapiens 10-15 2997281-5 1985 In addition, d(CH2)5Tyr(Et)VAVP blocked [3H]AVP binding to renal papillary membranes. Tritium 41-43 vasopressin-neurophysin 2-copeptin Oryctolagus cuniculus 28-31 2411868-2 1985 The incorporation of tritium from NaB3H4 into myelin proteins was confirmed by reaction with purified components of myelin basic protein or with lipophilin, a purified fraction of proteolipid protein. Tritium 21-28 proteolipid protein 1 Homo sapiens 145-155 4045551-2 1985 In this study, we have examined the action of CCK-peptides on the basal and potassium-evoked release of [3H]DA within this structure. Tritium 105-107 cholecystokinin Rattus norvegicus 46-49 4045551-6 1985 When sulfated CCK-octapeptide was administered into the NAc, it was found to increase the basal levels of [3H]DA released at concentrations of 2 X 10(-8) and 2 X 10(-7) M. However, at 2 X 10(-6) M there was no longer an effect by this peptide. Tritium 107-109 cholecystokinin Rattus norvegicus 14-17 23920221-8 2013 Regardless of the inhibitors tested, the most potent OATP1B1 inhibition was observed when [3H]E2G was used as a substrate. Tritium 91-93 solute carrier organic anion transporter family member 1B1 Homo sapiens 53-60 4045551-8 1985 In contrast to its effects on the basal release of [3H]DA, sulfated CCK-octapeptide was found to attenuate the potassium-evoked release of [3H]DA from the NAc in a concentration-dependent fashion from 2 X 10(-9) to 2 X 10(-6) M. The unsulfated form of the octapeptide had no effect on evoked release. Tritium 140-142 cholecystokinin Rattus norvegicus 68-71 3827860-6 1986 Activity of dihydroxyacetone-phosphate acyltransferase, but not glycerol-3-phosphate acyltransferase, was decreased in type II cells exposed to surfactant and this was reflected by a decrease in the 14C/3H ratio of total lipids synthesized when cells incubated with [U-14C]glycerol and [2-3H]glycerol were exposed to surfactant. Tritium 203-205 glyceronephosphate O-acyltransferase Homo sapiens 12-54 23915320-3 2013 RESULTS: We now show using RNA interference that CIP4 expression in neonatal rat ventricular myocytes is required for the induction of non-mitotic, hypertrophic growth by the alpha-adrenergic agonist phenylephrine, the IL-6 cytokine leukemia inhibitor factor, and fetal bovine serum, as assayed using morphometry, immunocytochemistry for the hypertrophic marker atrial natriuretic factor and [3H]leucine incorporation for de novo protein synthesis. Tritium 393-395 thyroid hormone receptor interactor 10 Rattus norvegicus 49-53 3789148-0 1986 Neurotensin stimulates [3H]oleic acid translocation across rat small intestine. Tritium 24-26 neurotensin Rattus norvegicus 0-11 2947981-4 1986 The "non-5-HT1A" sites labeled by 3H-5-HT in the presence of 0.1 microM 8-OH-DPAT corresponded mainly to 5-HT1B sites. Tritium 34-36 5-hydroxytryptamine receptor 1B Rattus norvegicus 105-111 4040531-5 1985 In the case of [3H]EqS and [3H]E1S, binding to SHBG was not detectable, and only a peak of radioactivity associated with albumin was found. Tritium 16-18 sex hormone binding globulin Equus caballus 47-51 4040531-5 1985 In the case of [3H]EqS and [3H]E1S, binding to SHBG was not detectable, and only a peak of radioactivity associated with albumin was found. Tritium 28-30 sex hormone binding globulin Equus caballus 47-51 4040531-11 1985 The binding constants of some estrogens and androgens to SHBG at 37 C determined by competitive Scatchard analysis using DEAE filter assay and [3H]5 alpha-DHT were 0.15, 0.07, 0.22, 0.29, 2.70, and 4.53 (X 10(9) M-1) for Eq, E1, 17 beta-Eq, E2, T, and 5 alpha-DHT, respectively. Tritium 144-146 sex hormone binding globulin Equus caballus 57-61 2997666-0 1985 Regional differences in mu 1-binding of [3H][D-Ala2,D-Leu5]-enkephalin: comparisons of thalamus and cortex in the rat. Tritium 41-43 sodium voltage-gated channel alpha subunit 4 Rattus norvegicus 24-28 23664345-12 2013 The time spent in SAG 3-5 increased between 1 and 3h before MTE and the prestand period (3 vs. 8%) and reached a peak level during STE. Tritium 50-52 S-antigen visual arrestin Bos taurus 18-21 4031660-1 1985 Supernatant protein factor (SPF) and anionic phospholipids such as phosphatidylglycerol (PG) stimulate squalene epoxidase activity in rat liver microsomes by promoting [3H]squalene uptake as well as substrate translocation (Chin, J., and K. Bloch. Tritium 169-171 SEC14-like lipid binding 2 Rattus norvegicus 0-26 4031660-1 1985 Supernatant protein factor (SPF) and anionic phospholipids such as phosphatidylglycerol (PG) stimulate squalene epoxidase activity in rat liver microsomes by promoting [3H]squalene uptake as well as substrate translocation (Chin, J., and K. Bloch. Tritium 169-171 SEC14-like lipid binding 2 Rattus norvegicus 28-31 3888998-5 1985 The enzyme degraded [3H]elastin and both elastin and trypsin-labile glycoproteins in a rat vascular smooth muscle extracellular matrix. Tritium 21-23 elastin Rattus norvegicus 24-31 4015090-1 1985 Concentrations of T-2, HT-2, 3"-OH T-2, 3"-OH HT-2, T-2 triol, and T-2 tetraol toxins which inhibited [3H]thymidine uptake in mitogen-stimulated human peripheral lymphocytes by 50% were 1.5, 3.5, 4.0, 50, 150, and 150 ng/ml, respectively. Tritium 103-105 solute carrier family 25 member 5 Homo sapiens 18-21 4015090-1 1985 Concentrations of T-2, HT-2, 3"-OH T-2, 3"-OH HT-2, T-2 triol, and T-2 tetraol toxins which inhibited [3H]thymidine uptake in mitogen-stimulated human peripheral lymphocytes by 50% were 1.5, 3.5, 4.0, 50, 150, and 150 ng/ml, respectively. Tritium 103-105 solute carrier family 25 member 5 Homo sapiens 24-27 4015090-1 1985 Concentrations of T-2, HT-2, 3"-OH T-2, 3"-OH HT-2, T-2 triol, and T-2 tetraol toxins which inhibited [3H]thymidine uptake in mitogen-stimulated human peripheral lymphocytes by 50% were 1.5, 3.5, 4.0, 50, 150, and 150 ng/ml, respectively. Tritium 103-105 solute carrier family 25 member 5 Homo sapiens 24-27 3540937-8 1986 The labeled duplex containing the poly(dC) template, [32P]-GTP, and [3H]dGTP loses 80% of the 32P label and 70% of the 3H label after treatment with 0.3 M KOH and DNase I, respectively. Tritium 69-71 deoxyribonuclease 1 Rattus norvegicus 163-170 3540937-8 1986 The labeled duplex containing the poly(dC) template, [32P]-GTP, and [3H]dGTP loses 80% of the 32P label and 70% of the 3H label after treatment with 0.3 M KOH and DNase I, respectively. Tritium 119-121 deoxyribonuclease 1 Rattus norvegicus 163-170 3814706-6 1986 In contrast, [3H]DHP levels in the endometrium were higher (p less than 0.01) than values in myometrium or plasma. Tritium 14-16 dihydropyrimidinase Rattus norvegicus 17-20 3814706-7 1986 Compared to values in the estrous cycle, endometrial ratios of [3H]DHP/[3H]P4 and [3H]metabolites/[3H]P4 decreased (p less than 0.02) on Days 3-5 of PSP. Tritium 64-66 dihydropyrimidinase Rattus norvegicus 67-70 3019772-2 1986 phorbol myristate acetate, mezerein, and rac-1-O-myristoyl-2-acetylglycerol, inhibited human neutrophil binding of [3H] leukotriene B4 (LTB4) by reducing the number of high-affinity receptors available to the arachidonic acid metabolite. Tritium 116-118 Rac family small GTPase 1 Homo sapiens 41-46 4015090-1 1985 Concentrations of T-2, HT-2, 3"-OH T-2, 3"-OH HT-2, T-2 triol, and T-2 tetraol toxins which inhibited [3H]thymidine uptake in mitogen-stimulated human peripheral lymphocytes by 50% were 1.5, 3.5, 4.0, 50, 150, and 150 ng/ml, respectively. Tritium 103-105 solute carrier family 25 member 5 Homo sapiens 24-27 23685729-6 2013 SULT1E1 activity was indicated by counting the transformed 3H-estradiol sulfate from 3H-labeled 17beta-estradiol added into the cell culture medium. Tritium 59-61 sulfotransferase family 1E member 1 Homo sapiens 0-7 3858600-2 1985 DNA radioactivity and quantitative estimation of autoradiographs with the use of [3H]thymidine revealed a significant increase in DNA content (twofold) and in the percentage of labeled neoplastic nuclei in mice treated with PRL plus MCA (48.50%) as compared to that in mice treated with MCA alone (23.50%). Tritium 82-84 prolactin Mus musculus 224-227 3023108-3 1986 Furthermore, xenopsin, LANT-6 and neuromedin N each inhibited the specific binding of [3H]neurotensin to intact N1E-115 cells in a dose-related fashion. Tritium 87-89 neurotensin Mus musculus 34-46 3790504-6 1986 From previously measured isotope effects, we predict the loss of tritium from the 1(R)-2H and 1(S)-2H samples to be 74:8 for a syn relationship between cleavage at C-1 and C-2 vs. 21:90 for an anti relationship. Tritium 65-72 complement C2 Bos taurus 164-175 23454239-6 2013 Cytochalasin D selectively inhibited the recycling of endocytosed B2R-GFP (B2R-GFP imaging, [3H]BK binding). Tritium 93-95 bradykinin receptor B2 Homo sapiens 66-69 3767382-7 1986 The kinetics of [3H]E2 association and dissociation at 4 degrees C are very rapid, with t1/2 values of less than 5 s. Sodium molybdate, generally used to stabilize steroid receptors, inhibits MEB-[3H]estradiol binding activity in cytosol in a time- and dose-dependent manner, an effect not observed with partially purified MEB. Tritium 17-19 protein O-linked mannose N-acetylglucosaminyltransferase 1 (beta 1,2-) Homo sapiens 192-195 3767382-7 1986 The kinetics of [3H]E2 association and dissociation at 4 degrees C are very rapid, with t1/2 values of less than 5 s. Sodium molybdate, generally used to stabilize steroid receptors, inhibits MEB-[3H]estradiol binding activity in cytosol in a time- and dose-dependent manner, an effect not observed with partially purified MEB. Tritium 17-19 protein O-linked mannose N-acetylglucosaminyltransferase 1 (beta 1,2-) Homo sapiens 323-326 3767382-7 1986 The kinetics of [3H]E2 association and dissociation at 4 degrees C are very rapid, with t1/2 values of less than 5 s. Sodium molybdate, generally used to stabilize steroid receptors, inhibits MEB-[3H]estradiol binding activity in cytosol in a time- and dose-dependent manner, an effect not observed with partially purified MEB. Tritium 197-199 protein O-linked mannose N-acetylglucosaminyltransferase 1 (beta 1,2-) Homo sapiens 192-195 3773520-2 1986 The equilibrium dissociation constant for the interaction of [3H]RU38486 with the molybdate-stabilized glucocorticoid receptor was lower than that for [1,2,4-3H]triamcinolone acetonide at 0 degree C but higher at 25 degrees C, suggesting that hydrophobic interactions play a major role in the binding of RU38486. Tritium 62-64 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 103-126 2863760-5 1985 [3H]-YM-09151-2 binding was inhibited by dopaminergic drugs in a concentration and stereoselective manner with the appropriate dopamine D2 receptor profile. Tritium 1-3 dopamine receptor D2 Canis lupus familiaris 127-147 3987974-10 1985 Nonenzymatic glycation of fibronectin in vitro resulted in markedly decreased binding of 3H-fibronectin to collagen. Tritium 89-91 fibronectin 1 Canis lupus familiaris 92-103 3921021-4 1985 ADP-ribosylation by toxin of transducin, the retinal GTP-binding protein which is similar in structure and function to that in cyclase, decreased [3H]Gpp(NH)p binding. Tritium 147-149 guanine nucleotide binding protein, alpha transducing 1 Mus musculus 29-39 23314484-1 2013 A simple, selective, and sensitive method utilizing tritium ((3)H) release from (3)H-deoxyuridine 5"-monophosphate (dUMP) substrate for accurate and precise determination of the low basal thymidylate synthase activity (TSA) in normal healthy peripheral blood mononuclear cells (PBMCs) was developed and validated. Tritium 52-59 thymidylate synthetase Homo sapiens 188-208 3994696-1 1985 A simple sensitive method for the determination of the initial rate of the reaction of lecithin-cholesterol acyltransferase by equilibrating [3H]cholesterol with unesterified cholesterol of human serum is described. Tritium 142-144 lecithin-cholesterol acyltransferase Homo sapiens 87-123 3773520-4 1986 The rate of dissociation of [3H]RU38486 from the glucocorticoid receptor was higher in the absence of molybdate than in its presence both at 0 degree C and at 25 degrees C, suggesting that molybdate modifies the physical state of the antagonist-receptor complex, but other physical properties were similar both in the presence and in the absence of molybdate. Tritium 29-31 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 49-72 3773520-5 1986 The rate of inactivation of the unoccupied glucocorticoid receptor at 25 degrees C in the absence of molybdate was lower in phosphate buffer than in Tris-HCl buffer but the rate of dissociation of [3H]RU38486 was the same in both buffers. Tritium 198-200 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 43-66 3018478-0 1986 Characterization of the binding of [3H]-(+/-)-L-364,718: a new potent, nonpeptide cholecystokinin antagonist radioligand selective for peripheral receptors. Tritium 36-38 cholecystokinin Rattus norvegicus 82-97 3018478-1 1986 [3H]-(+/-)-L-364,718 a new, potent and selective nonpeptide peripheral cholecystokinin (CCK) antagonist bound saturably and reversibly to rat pancreatic membranes. Tritium 1-3 cholecystokinin Rattus norvegicus 71-86 3018478-1 1986 [3H]-(+/-)-L-364,718 a new, potent and selective nonpeptide peripheral cholecystokinin (CCK) antagonist bound saturably and reversibly to rat pancreatic membranes. Tritium 1-3 cholecystokinin Rattus norvegicus 88-91 3018478-4 1986 The rank order of potency of various CCK agonists and antagonists in displacing [3H]-(+/-)-L-364,718 correlated with their ability to displace [125I]CCK-8 and their known pharmacological activities in peripheral tissues. Tritium 81-83 cholecystokinin Rattus norvegicus 37-40 3018478-4 1986 The rank order of potency of various CCK agonists and antagonists in displacing [3H]-(+/-)-L-364,718 correlated with their ability to displace [125I]CCK-8 and their known pharmacological activities in peripheral tissues. Tritium 81-83 cholecystokinin Rattus norvegicus 149-152 3018478-7 1986 [3H]-(+/-)-L-364,718 also demonstrated specific binding to bovine gall bladder tissue but not guinea pig brain or gastric glands, consistent with its selectivity as a peripheral CCK antagonist. Tritium 1-3 cholecystokinin Cavia porcellus 178-181 3018478-9 1986 These data indicate that [3H]-(+/-)-L-364,718 represents a new potent nonpeptide antagonist radioligand for the study of peripheral CCK receptors which may allow differentiation of agonist and antagonist interactions. Tritium 26-28 cholecystokinin Rattus norvegicus 132-135 3020583-3 1986 The products of hydrolysis of LTA3, LTA4 and LTA5 by human and rat neutrophil LTA4 hydrolase have been shown to displace [3H] LTB4 binding to human and rat neutrophil membranes. Tritium 122-124 leukotriene A4 hydrolase Rattus norvegicus 78-92 2862827-4 1985 As seen from simulation curves, CCK-8 increases the binding of [3H]DA agonists and reduces the binding of [3H]DA antagonists in striatal membranes, suggesting that CCK-8 may increase striatal DA transmission. Tritium 64-66 cholecystokinin Rattus norvegicus 32-35 2862827-4 1985 As seen from simulation curves, CCK-8 increases the binding of [3H]DA agonists and reduces the binding of [3H]DA antagonists in striatal membranes, suggesting that CCK-8 may increase striatal DA transmission. Tritium 64-66 cholecystokinin Rattus norvegicus 164-167 2862827-4 1985 As seen from simulation curves, CCK-8 increases the binding of [3H]DA agonists and reduces the binding of [3H]DA antagonists in striatal membranes, suggesting that CCK-8 may increase striatal DA transmission. Tritium 107-109 cholecystokinin Rattus norvegicus 32-35 2862827-4 1985 As seen from simulation curves, CCK-8 increases the binding of [3H]DA agonists and reduces the binding of [3H]DA antagonists in striatal membranes, suggesting that CCK-8 may increase striatal DA transmission. Tritium 107-109 cholecystokinin Rattus norvegicus 164-167 2862827-6 1985 In the aged brain, the ability of CCK-8 to modulate DA antagonist binding sites is changed such that the binding of [3H]DA antagonists is increased. Tritium 117-119 cholecystokinin Rattus norvegicus 34-37 3161694-1 1985 Chymotrypsin can completely solubilize insoluble [3H]-labeled ligamentum nuchae elastin. Tritium 50-52 elastin Homo sapiens 80-87 3161694-6 1985 The sizes of [3H]elastin fragments produced by the elastolytic activity of chymotrypsin are similar to those produced by pancreatic elastase, and larger than those produced by trypsin. Tritium 14-16 elastin Homo sapiens 17-24 3526340-3 1986 We then measured catabolism of the [3H]S-protein-S-peptide conjugate after its microinjection into human diploid fibroblasts. Tritium 36-38 vitronectin Homo sapiens 39-48 23270998-2 2013 We carried out the uptake of [3H]-tyrosine to assess the functional activity of LAT1. Tritium 29-33 solute carrier family 7 member 5 Homo sapiens 80-84 3758193-3 1986 We determined the short-term and long-term competition of cyproterone acetate and chlormadinone acetate with [3H]DHT for receptor binding at 0 degrees C and showed, that the complexes formed by these antiandrogens with the androgen receptor have equally reduced stabilities compared to the DHT-receptor complex. Tritium 110-112 androgen receptor Rattus norvegicus 223-240 3459918-7 1986 Maximum binding to the immunoaffinity column was obtained after DNase 1 digestion of [3H]BPDE-DNA: The bound adducts could be readily eluted from the column with 50 mM NaOH. Tritium 86-88 deoxyribonuclease I Mus musculus 64-71 3749001-2 1986 Samples of full-term human placenta were incubated for up to 3 h with 125I-RBP or [3H]retinol-RBP. Tritium 83-85 retinol binding protein 4 Homo sapiens 94-97 3884352-12 1985 Dose-response curves indicated that HCF in culture were very sensitive to EGF, insulin, and FGF with maximum stimulation of [3H]-thymidine incorporation occurring at approximately 1 nM for EGF and insulin and at 100 micrograms ml-1 for FGF. Tritium 125-127 host cell factor C1 Homo sapiens 36-39 4066774-4 1985 Polyacrylamide gel analysis of immunoprecipitated [3H]-leucine- and [3H]-glucosamine-labeled ECM from the human glioma cell line U-251MG has shown that GMEM is a high-molecular-weight macromolecule (Mr approximately 1,000,000) composed of Mr approximately 230,000 disulfide-bonded glycoprotein subunits. Tritium 51-53 tenascin C Homo sapiens 152-156 4066774-4 1985 Polyacrylamide gel analysis of immunoprecipitated [3H]-leucine- and [3H]-glucosamine-labeled ECM from the human glioma cell line U-251MG has shown that GMEM is a high-molecular-weight macromolecule (Mr approximately 1,000,000) composed of Mr approximately 230,000 disulfide-bonded glycoprotein subunits. Tritium 69-71 tenascin C Homo sapiens 152-156 3931095-7 1985 Nearly all of the IRBP secreted by bovine retinas incubated with (3H)-leucine in the presence of tunicamycin was nonglycosylated and did not bind to concanavalin A. Tritium 66-68 retinol binding protein 3 Bos taurus 18-22 23270998-9 2013 Isoleucine-quinidine (Ile-quinidine) prodrug generates a significant inhibitory effect on [3H]-tyrosine uptake suggesting that it is recognized by LAT1. Tritium 91-93 solute carrier family 7 member 5 Homo sapiens 147-151 6501305-3 1984 Gel filtration of a reaction mixture on Sephadex G-75 in the presence of 0.15 M NaCl showed that the 3H-labeled alpha-chains in Hb A were an integral part of, and not nonspecifically adsorbed to, the tetramer. Tritium 101-103 sodium voltage-gated channel alpha subunit 2 Homo sapiens 128-132 3087977-4 1986 The enzyme activity of the cytosolic LTC4-binding protein was inhibited by submicromolar quantities of unlabeled LTC4, and the binding activity for [3H]LTC4 was blocked by the ligandin substrates, hematin and bilirubin. Tritium 149-151 glutathione S-transferase alpha 2 Rattus norvegicus 176-184 6501305-4 1984 These results indicate that the free 3H-labeled alpha chain monomers were incorporated into Hb A by exchange with pre-existing unlabeled alpha chain subunits according to the scheme, alpha 2 beta 2 in equilibrium 2 alpha beta in equilibrium 2 alpha + 2 beta. Tritium 37-39 sodium voltage-gated channel alpha subunit 2 Homo sapiens 92-96 23174481-6 2013 Zebrafish larvae exposed to Cd for 3h showed increased glutathione S-transferase pi (gst pi), glutamate-cysteine ligase catalytic subunit (gclc), heme oxygenase 1 (hmox1) and peroxiredoxin 1 (prdx1) mRNA levels indicative of Nrf2 activation, and which were blocked by morpholino-mediated Nrf2 knockdown. Tritium 35-37 peroxiredoxin 1 Danio rerio 175-190 23174481-6 2013 Zebrafish larvae exposed to Cd for 3h showed increased glutathione S-transferase pi (gst pi), glutamate-cysteine ligase catalytic subunit (gclc), heme oxygenase 1 (hmox1) and peroxiredoxin 1 (prdx1) mRNA levels indicative of Nrf2 activation, and which were blocked by morpholino-mediated Nrf2 knockdown. Tritium 35-37 peroxiredoxin 1 Danio rerio 192-197 6098330-2 1984 One day after the last of 3 class 4-5 kindled seizures, the specific binding of [3H]kainic acid in stratum lucidum of area CA3 was 47-61% less than in electrode-implanted unstimulated controls. Tritium 81-84 carbonic anhydrase 3 Rattus norvegicus 123-126 6098333-2 1984 This effect was tetrodotoxin-resistant and a good correlation was noticed between the magnitude of neurotensin-stimulated inositol phospholipid hydrolysis and the number of specific [3H]neurotensin binding sites in various brain regions. Tritium 183-185 neurotensin Rattus norvegicus 99-110 6098333-2 1984 This effect was tetrodotoxin-resistant and a good correlation was noticed between the magnitude of neurotensin-stimulated inositol phospholipid hydrolysis and the number of specific [3H]neurotensin binding sites in various brain regions. Tritium 183-185 neurotensin Rattus norvegicus 186-197 6083874-3 1984 Neurotensin, however, augmented the increase in [3H]spiroperidol binding caused by the haloperidol treatment in both the nucleus accumbens and striatum. Tritium 49-51 neurotensin Rattus norvegicus 0-11 6092561-6 1984 The portion of [3H]-beta-endorphin binding that is inhibited under these conditions appears to be equally sensitive to both morphine and the enkephalin and may correspond to mu1 sites. Tritium 16-18 sodium voltage-gated channel alpha subunit 4 Rattus norvegicus 174-177 6092561-8 1984 This possible binding of [3H]-beta-endorphin to mu1 sites is consistent with the role of mu1 sites in beta-endorphin analgesia and catalepsy in vivo. Tritium 26-28 sodium voltage-gated channel alpha subunit 4 Rattus norvegicus 48-51 3084473-8 1986 Uninduced cells exhibited enhanced incorporation of [3H]alkyllyso-GPC or [3H]alkylacetyl-GPC into alkylacyl-GPC and of [14C]arachidonate and [14C]oleate into various phospholipids including phosphatidylcholine. Tritium 52-55 glycophorin C (Gerbich blood group) Homo sapiens 66-69 2422345-6 1986 Veratridine, A23187 and elevated potassium ions decreased the levels of ATP and [3H]AdoMet. Tritium 81-83 methionine adenosyltransferase 1A Rattus norvegicus 84-90 2422345-7 1986 The A23187-mediated decrease in ATP levels and the reduced [3H]AdoMet formation was antagonized by ethylene glycol bis(beta-aminoethyl ether)-N,N"-tetraacetic acid and MnCl2. Tritium 60-62 methionine adenosyltransferase 1A Rattus norvegicus 63-69 23174481-6 2013 Zebrafish larvae exposed to Cd for 3h showed increased glutathione S-transferase pi (gst pi), glutamate-cysteine ligase catalytic subunit (gclc), heme oxygenase 1 (hmox1) and peroxiredoxin 1 (prdx1) mRNA levels indicative of Nrf2 activation, and which were blocked by morpholino-mediated Nrf2 knockdown. Tritium 35-37 nfe2 like bZIP transcription factor 2a Danio rerio 225-229 2422345-8 1986 Inhibition of metabolic activity of the synaptosomes by NaCN led to: decreased ATP levels; inhibition of [3H]AdoMet formation; and inhibition of PCM activity. Tritium 106-108 methionine adenosyltransferase 1A Rattus norvegicus 109-115 6736671-6 1984 FCS was found to contain calmodulin-like activity at concentrations that may be relevant to the stimulation of [3H]thymidine uptake by cells in culture. Tritium 112-114 calmodulin 2 Mus musculus 25-35 23174481-6 2013 Zebrafish larvae exposed to Cd for 3h showed increased glutathione S-transferase pi (gst pi), glutamate-cysteine ligase catalytic subunit (gclc), heme oxygenase 1 (hmox1) and peroxiredoxin 1 (prdx1) mRNA levels indicative of Nrf2 activation, and which were blocked by morpholino-mediated Nrf2 knockdown. Tritium 35-37 nfe2 like bZIP transcription factor 2a Danio rerio 288-292 23635403-3 2013 We used in situ brain perfusion to show that rosuvastatin and taurocholate, two established Oatp1a4 substrates, decreased (5-fold) the Clup of [3H]Abeta while L-thyroxine increased it (5.5-fold). Tritium 144-146 solute carrier organic anion transporter family, member 1a4 Mus musculus 92-99 6727566-5 1984 At 10 wks of age 3H-PRL synthesis was significantly decreased by these doses of DES and HPC. Tritium 17-19 prolactin Mus musculus 20-23 6202319-11 1984 Two alpha 2M-kallikrein light chain complexes incorporated [3H]diisopropyl fluorophosphate ( [3H]DFP) whereas the Mr 330 000 complex did not react with [3H]DFP. Tritium 60-62 kallikrein related peptidase 4 Homo sapiens 13-23 3009562-3 1986 The distribution of 3H-CHA binding was consistent among the species with the strata radiatum and oriens of fields CA1 and CA3 exhibiting the highest levels of binding. Tritium 20-22 carbonic anhydrase 1 Homo sapiens 114-117 23635403-3 2013 We used in situ brain perfusion to show that rosuvastatin and taurocholate, two established Oatp1a4 substrates, decreased (5-fold) the Clup of [3H]Abeta while L-thyroxine increased it (5.5-fold). Tritium 144-146 amyloid beta (A4) precursor protein Mus musculus 147-152 23183626-5 2013 The selective CRF(2) agonist, Ucn 2 (3 mug/kg, iv) increased fasting acyl (3h: 49%) and des-acyl ghrelin levels (3h: 30%) compared to vehicle while the preferential CRF(1) agonist, CRF (3 mug/kg, iv) had no effect. Tritium 75-77 urocortin 2 Rattus norvegicus 14-35 3527152-3 1986 The antibody indirectly precipitates [3H]pargyline-labelled human MAO B both from liver and platelet extracts but fails to precipitate MAO A from liver extracts. Tritium 38-40 monoamine oxidase B Homo sapiens 66-71 6696933-2 1984 Kinetically, three apparent Km values for S-adenosyl-L-methionine (AdoMet) were obtained when the total [3H]methyl groups incorporation into the phospholipids was examined in the presence of 0.01-250 microM AdoMet. Tritium 105-107 methionine adenosyltransferase 1A Rattus norvegicus 42-65 23183626-5 2013 The selective CRF(2) agonist, Ucn 2 (3 mug/kg, iv) increased fasting acyl (3h: 49%) and des-acyl ghrelin levels (3h: 30%) compared to vehicle while the preferential CRF(1) agonist, CRF (3 mug/kg, iv) had no effect. Tritium 113-115 urocortin 2 Rattus norvegicus 14-35 6319699-2 1984 The resulting analogue, 12, had 1/4000th and 1/2400th the opiate receptor binding activity of Leu-enkephalin when (3H) [D-Ala2,D-Leu5]enkephalin and (3H)naloxone, respectively, were used as tritiated ligands. Tritium 114-118 prodynorphin Mus musculus 94-108 3005505-8 1986 Thus, the percent of receptors appearing in the lysosomal fraction that could still bind [3H]D-Ala2-D-Leu5-enkephalin in vitro was increased on treatment with Leu-enkephalin. Tritium 90-92 prodynorphin Mus musculus 159-173 22560901-6 2012 These compounds induced apoptosis and activated the p53 pathway in zebrafish within 3h treatment. Tritium 84-86 tumor protein p53 Danio rerio 52-55 3457370-5 1986 Approximately 50% of the [3H]mannose incorporated into ovalbumin secreted by L cells is found in such hybrid structures. Tritium 26-28 ovalbumin (SERPINB14) Gallus gallus 55-64 6229533-3 1984 Ninety-one per cent of the 3H counts present in the oligonucleosomes specifically bound to the anti-HMG-17 column. Tritium 27-29 high mobility group nucleosomal binding domain 2 Homo sapiens 100-106 22351476-9 2012 Co-administration of erythropoietin and insulin resulted in higher phosphorylation of Akt and [3H]-deoxy-D-glucose uptake in adipocytes than insulin treatment alone. Tritium 95-97 erythropoietin Rattus norvegicus 21-35 6329122-4 1984 This pentapeptide (DAc- Glu3 -TP5) affected the cAMP and cGMP levels positively and decreased the 3H-thymidine incorporation in lymphocyte cultures significantly. Tritium 98-100 dachshund family transcription factor 1 Mus musculus 19-22 6373133-1 1984 We developed an assay for measurement of elastolytic activity using insoluble 3H-labelled particulate elastin adherent to plastic that is capable of detecting 150 picograms of pancreatic elastase. Tritium 78-80 elastin Homo sapiens 102-109 6376085-4 1984 For in vitro exposures, PRP were incubated with 3H-thymidine (3H-TdR) in the presence of genotoxic agents for 18-22 hr. Tritium 48-50 proline rich protein 2-like 1 Rattus norvegicus 24-27 3008720-1 1986 Exposure of [3H]-lysine labeled elastin to either purified myeloperoxidase plus H2O2 and halides or human neutrophils plus phorbol myristate acetate resulted in oxidation of lysine side chains quantitated as 3H2O release. Tritium 13-15 elastin Homo sapiens 32-39 3723066-4 1986 In contrast, [3H]albumin could not be found in either tissue extract. Tritium 14-16 albumin Rattus norvegicus 17-24 3715057-3 1986 The present study reports an evaluation of the kinetic isotope effect which occurs during the oxidation of 15-hydroxyl group of tritium-labeled prostaglandins E2 and F2 alpha by the 15-hydroxyprostaglandin dehydrogenase and during the oxidation of 9-hydroxyl group of tritium-labeled prostaglandin F2 alpha by the 9-hydroxyprostaglandin dehydrogenase. Tritium 128-135 carbonyl reductase 1 Homo sapiens 182-219 3715057-3 1986 The present study reports an evaluation of the kinetic isotope effect which occurs during the oxidation of 15-hydroxyl group of tritium-labeled prostaglandins E2 and F2 alpha by the 15-hydroxyprostaglandin dehydrogenase and during the oxidation of 9-hydroxyl group of tritium-labeled prostaglandin F2 alpha by the 9-hydroxyprostaglandin dehydrogenase. Tritium 268-275 carbonyl reductase 1 Homo sapiens 182-219 6523062-5 1984 Decreased amounts of 3H-labeling of glycoprotein IIa as well as absence of the specific surface glycoprotein Ib was observed in Bernard-Soulier platelets. Tritium 21-23 integrin subunit beta 1 Homo sapiens 36-52 22391220-6 2012 We showed that ABCG2 and Abcg4 mediate the cellular efflux of [3H] Abeta1-40. Tritium 63-65 ATP binding cassette subfamily G member 4 Mus musculus 25-30 2416880-5 1986 Incubation of the membrane fraction with [3H]palmitoyl-CoA in the absence of CoA and ATP led to highly labeled PLP. Tritium 42-44 proteolipid protein 1 Homo sapiens 111-114 22391220-7 2012 In addition, probucol fully inhibited the efflux of [3H] Abeta1-40 from HEK293-abcg4 cells. Tritium 53-55 ATP binding cassette subfamily G member 4 Mus musculus 79-84 6317487-2 1983 Some pituitary glands were cultured with 3H-labelled leucine for 24 h to obtain radioactive prolactin. Tritium 41-43 prolactin Mus musculus 92-101 22391220-9 2012 Perfusing the brains of Abcb1/Abcg2- and Abca1-deficient mice with [3H] Abeta1-40 plus probucol significantly increased the Clup of Abeta. Tritium 68-70 amyloid beta (A4) precursor protein Mus musculus 72-77 3941318-3 1986 This distribution of [3H]HC-3 binding sites was in close agreement with the amounts of choline acetyltransferase in specific nuclei and subregions of rat brain. Tritium 22-24 choline O-acetyltransferase Rattus norvegicus 87-112 21734099-7 2011 Consistent with this, aquaporin-2 (AQP2) expression in the PM fractions of D5d and D5d-3h increased, whereas AQP2 expression in the ICV fractions of D5d-3h was further increased, indicating internalization of AQP2. Tritium 87-89 aquaporin 2 Rattus norvegicus 22-33 3564084-0 1986 Binding of (3H)7 alpha,17 alpha-dimethyl-19-nortestosterone (mibolerone) to progesterone receptors: comparison with binding of (3H)R5020 and (3H)ORG2058. Tritium 12-14 progesterone receptor Bos taurus 76-98 3564084-1 1986 The binding characteristics of (3H)7 alpha,17 alpha-dimethyl-19-nortestosterone [3H)DMNT) to progesterone receptors (PgR) of calf uterine tissue cytosol were determined and compared to those of the synthetic progestins (3H)ORG2058 and (3H)R5020. Tritium 32-34 progesterone receptor Bos taurus 93-115 3564084-1 1986 The binding characteristics of (3H)7 alpha,17 alpha-dimethyl-19-nortestosterone [3H)DMNT) to progesterone receptors (PgR) of calf uterine tissue cytosol were determined and compared to those of the synthetic progestins (3H)ORG2058 and (3H)R5020. Tritium 32-34 progesterone receptor Bos taurus 117-120 6318184-1 1983 Protein kinase activity that is dependent on 3",5"-Cyclic adenosine monophosphate (cAMP-PK), [3H]cAMP binding, and cAMP-dependent protein phosphorylation were identified and partially characterized in cytosolic preparations of rat lung from day 18 of gestation to adulthood. Tritium 94-96 KIT proto-oncogene receptor tyrosine kinase Rattus norvegicus 0-14 6318184-4 1983 Inhibition of specific [3H]cAMP binding to lung cytosol (to the regulatory subunit of the cAMP-dependent protein kinase) followed the order of potency: cAMP greater than cGMP; adenosine, ADP, and ATP were inactive. Tritium 24-26 KIT proto-oncogene receptor tyrosine kinase Rattus norvegicus 105-119 3564084-1 1986 The binding characteristics of (3H)7 alpha,17 alpha-dimethyl-19-nortestosterone [3H)DMNT) to progesterone receptors (PgR) of calf uterine tissue cytosol were determined and compared to those of the synthetic progestins (3H)ORG2058 and (3H)R5020. Tritium 81-83 progesterone receptor Bos taurus 93-115 21734099-7 2011 Consistent with this, aquaporin-2 (AQP2) expression in the PM fractions of D5d and D5d-3h increased, whereas AQP2 expression in the ICV fractions of D5d-3h was further increased, indicating internalization of AQP2. Tritium 87-89 aquaporin 2 Rattus norvegicus 35-39 3564084-1 1986 The binding characteristics of (3H)7 alpha,17 alpha-dimethyl-19-nortestosterone [3H)DMNT) to progesterone receptors (PgR) of calf uterine tissue cytosol were determined and compared to those of the synthetic progestins (3H)ORG2058 and (3H)R5020. Tritium 81-83 progesterone receptor Bos taurus 117-120 24310499-7 2011 The accumulation of [3H]-3TC, -ddI and -TDF were temperature sensitive (suggesting facilitated transport), in contrast to [3H]-ABC. Tritium 21-23 sex determining region Y Homo sapiens 40-43 3564084-1 1986 The binding characteristics of (3H)7 alpha,17 alpha-dimethyl-19-nortestosterone [3H)DMNT) to progesterone receptors (PgR) of calf uterine tissue cytosol were determined and compared to those of the synthetic progestins (3H)ORG2058 and (3H)R5020. Tritium 81-83 progesterone receptor Bos taurus 93-115 3564084-1 1986 The binding characteristics of (3H)7 alpha,17 alpha-dimethyl-19-nortestosterone [3H)DMNT) to progesterone receptors (PgR) of calf uterine tissue cytosol were determined and compared to those of the synthetic progestins (3H)ORG2058 and (3H)R5020. Tritium 81-83 progesterone receptor Bos taurus 117-120 3564084-1 1986 The binding characteristics of (3H)7 alpha,17 alpha-dimethyl-19-nortestosterone [3H)DMNT) to progesterone receptors (PgR) of calf uterine tissue cytosol were determined and compared to those of the synthetic progestins (3H)ORG2058 and (3H)R5020. Tritium 81-83 progesterone receptor Bos taurus 93-115 3564084-1 1986 The binding characteristics of (3H)7 alpha,17 alpha-dimethyl-19-nortestosterone [3H)DMNT) to progesterone receptors (PgR) of calf uterine tissue cytosol were determined and compared to those of the synthetic progestins (3H)ORG2058 and (3H)R5020. Tritium 81-83 progesterone receptor Bos taurus 117-120 6580640-5 1983 The ornithine decarboxylase inhibitor alpha-difluoromethylornithine (5 mM) suppressed the testosterone-induced increase in polyamine levels and rates of endocytosis, hexose transport, and amino acid transport, measured by horseradish peroxidase, [14C]aminoisobutyric acid, and deoxy[3H]glucose uptake. Tritium 283-285 ornithine decarboxylase, structural 1 Mus musculus 4-27 6626979-1 1983 An in vitro assay procedure is described for measuring androgen receptor binding in cytosol and cell nuclei of brain and pituitary tissue using [3H]R1881 as ligand. Tritium 145-147 androgen receptor Rattus norvegicus 55-72 24310499-9 2011 ABC/TDF and ABC/ddI increased the accumulation of [3H]-3TC and 3TC/TDF also increased the accumulation of [3H]-TDF. Tritium 51-53 sex determining region Y Homo sapiens 4-7 6626979-3 1983 Competition and saturation analysis indicated that [3H]R1881 binding has the specificity and nanomolar affinity expected of an androgen receptor. Tritium 52-54 androgen receptor Rattus norvegicus 127-144 6310291-5 1983 A portion of the higher affinity (KD 0.06 nM) component was inhibited by naloxonazine treatment in vitro (50 nM), suggesting that 3H-Mr2034 bound with very high affinity to mu1 sites. Tritium 130-132 sodium voltage-gated channel alpha subunit 4 Rattus norvegicus 173-176 6688713-2 1983 Increased ligandin content was accompanied by parallel increase in subunit synthesis as determined by [3H]leucine incorporation into each subunit relative to incorporation into total cytosolic proteins. Tritium 103-105 glutathione S-transferase alpha 2 Rattus norvegicus 10-18 6224755-2 1983 Retinol-binding protein (RBP) was isolated from human serum, its native ligand removed and replaced with [3H]-retinol. Tritium 106-108 retinol binding protein 4, plasma Mus musculus 0-23 3000953-8 1986 GIN-7 also detected p28 in 3H-glucosamine-labelled MT-2 cells. Tritium 27-29 golgi SNAP receptor complex member 1 Homo sapiens 20-23 3001304-6 1986 Only trans-4 weakly inhibited [3H]GABA binding to GABAA and GABAB receptors in vitro. Tritium 31-33 gamma-aminobutyric acid (GABA) A receptor, subunit gamma 1 Mus musculus 50-55 6224755-2 1983 Retinol-binding protein (RBP) was isolated from human serum, its native ligand removed and replaced with [3H]-retinol. Tritium 106-108 retinol binding protein 4, plasma Mus musculus 25-28 21338579-3 2011 The early transcription factor EGR1 (Early Growth Response gene 1) mRNA and protein levels peaked after 3h of incubation with 25muM TGZ, CGZ or 15d-PGJ(2) and then gradually decreased. Tritium 104-106 early growth response 1 Homo sapiens 31-35 6224755-3 1983 After reconstitution, [3H]-retinol-RBP was reassociated with prealbumin (PA), and the protein complex injected intravenously into dark-adapted animals. Tritium 23-25 retinol binding protein 4, plasma Mus musculus 35-38 6888374-7 1983 By contrast, treatment for 12 weeks with CCl4 was accompanied either by increased fidelity (decrease in the relative incorporation of [3H]dGMP) or no change in fidelity. Tritium 135-137 chemokine (C-C motif) ligand 4 Mus musculus 41-45 3001280-2 1986 To select a tissue homogenate rich for this site, we have characterized the binding of highly specific [3H]LTB4 to guinea-pig spleen membranes and we have determined the ability of LTB4 to compete with [3H]LTB4 for binding sites in the membranes of 10 nonspleen tissues. Tritium 104-106 prostaglandin reductase 1 Cavia porcellus 107-111 3001280-3 1986 In the spleen membrane, MgCl2 and CaCl2 enhanced [3H]LTB4 binding, but NaCl and KCl decreased it. Tritium 50-52 prostaglandin reductase 1 Cavia porcellus 53-57 3001280-4 1986 Spleen [3H] LTB4 binding was a function of protein concentration and was rapid, reversible, stereoselective and saturable. Tritium 8-10 prostaglandin reductase 1 Cavia porcellus 12-16 3001280-9 1986 Competition studies further indicated that felodipine, a 1,4-dihyropyridine Ca++ channel blocker, exhibited micromolar inhibition of spleen [3H]LTB4 binding. Tritium 141-143 prostaglandin reductase 1 Cavia porcellus 144-148 3585861-7 1986 Scatchard analysis of [3H]estradiol binding in rat liver cytosols shows a 50% reduction in receptor concentration in the PGF-treated group. Tritium 23-25 placental growth factor Rattus norvegicus 121-124 3585861-8 1986 Specific binding of [3H]estradiol in anterior pituitary was also suppressed by PGF treatment. Tritium 21-23 placental growth factor Rattus norvegicus 79-82 21338579-3 2011 The early transcription factor EGR1 (Early Growth Response gene 1) mRNA and protein levels peaked after 3h of incubation with 25muM TGZ, CGZ or 15d-PGJ(2) and then gradually decreased. Tritium 104-106 early growth response 1 Homo sapiens 37-65 6615438-1 1983 Ganglioside GM1, 3H-labelled at the level of terminal galactose or of sphingosine, was intravenously injected into Swiss albino mice and some steps in its metabolic fate in the liver were investigated. Tritium 17-19 coenzyme Q10A Mus musculus 12-15 6615438-2 1983 After administration of [3H]sphingosine-labelled GM1 all major liver gangliosides [GM3, GM2, GM1, GD1a-(NeuAc,NeuGl)] became radioactive, the radioactivity residing in all cases on the sphingosine moiety. Tritium 25-27 coenzyme Q10A Mus musculus 49-52 21316174-6 2011 The novel protocol is an accurate, easy-to-handle and robust screening assay for JNK3 and the assay performance was reduced from 7.5 to 3h. Tritium 136-138 mitogen-activated protein kinase 10 Homo sapiens 81-85 6615438-2 1983 After administration of [3H]sphingosine-labelled GM1 all major liver gangliosides [GM3, GM2, GM1, GD1a-(NeuAc,NeuGl)] became radioactive, the radioactivity residing in all cases on the sphingosine moiety. Tritium 25-27 granulocyte macrophage antigen 3 Mus musculus 83-86 6615438-2 1983 After administration of [3H]sphingosine-labelled GM1 all major liver gangliosides [GM3, GM2, GM1, GD1a-(NeuAc,NeuGl)] became radioactive, the radioactivity residing in all cases on the sphingosine moiety. Tritium 25-27 coenzyme Q10A Mus musculus 93-96 6411369-6 1983 When [3H]N-hydroxy-Trp-P-2 was incubated in the presence of pig liver lecithin, the polar products which were not formed in the incubation without lipids were newly detected by thin-layer chromatography (TLC) analysis. Tritium 6-8 polycystin 2, transient receptor potential cation channel Sus scrofa 19-26 6311560-1 1983 A model system is presented to explain how tyrosinase, an enzyme unique to pigmented cells such as normal and malignant melanocytes can oxidize [3H]-estradiol to radiolabeled products which closely resemble the tight binding of [3H]-estradiol to estrogen receptor. Tritium 145-147 tyrosinase Homo sapiens 43-53 3001463-0 1985 [3H] Boc [Nle28, 31]CCK27-33, a new highly labelled ligand for CCK receptors: binding on brain and on pancreas. Tritium 1-3 cholecystokinin Mus musculus 20-23 3001463-5 1985 Thus, [3H]Boc[diNle]CCK7 is a very promising new probe for the characterization of CCK receptors and their interaction with different CCK fragments. Tritium 7-9 cholecystokinin Mus musculus 20-23 3001463-5 1985 Thus, [3H]Boc[diNle]CCK7 is a very promising new probe for the characterization of CCK receptors and their interaction with different CCK fragments. Tritium 7-9 cholecystokinin Mus musculus 83-86 2999126-3 1985 It potently inhibits [3H]PAF (1 nM) binding to receptor sites on rabbit platelet membranes with an ED50 of 2 X 10(-8) M under the assay condition without the addition of mono- or divalent cations. Tritium 22-24 PCNA clamp associated factor Rattus norvegicus 25-28 6311560-1 1983 A model system is presented to explain how tyrosinase, an enzyme unique to pigmented cells such as normal and malignant melanocytes can oxidize [3H]-estradiol to radiolabeled products which closely resemble the tight binding of [3H]-estradiol to estrogen receptor. Tritium 229-231 tyrosinase Homo sapiens 43-53 4067325-2 1985 [3H]Retinol bound to retinol-binding protein (RBP) was incubated for 24 h with the tissue sample, which was then extracted and analyzed by high-performance liquid chromatography. Tritium 1-3 retinol binding protein 4 Homo sapiens 21-44 6311560-2 1983 In the model system studied, tyrosinase oxidized [2,4,6,7-3H]-estradiol to [3H]-water and [3H]-estradiol metabolites, the latter of which formed ring-substituted conjugates with nucleophiles like monothioglycerol and BSA. Tritium 58-60 tyrosinase Homo sapiens 29-39 21256203-7 2011 For exploring the carcinogenesis mechanism, we incubated cells with [3H]BaP and determined BaP-DNA binding activity by measuring its radioactivity. Tritium 69-71 prohibitin 2 Homo sapiens 72-75 6311560-2 1983 In the model system studied, tyrosinase oxidized [2,4,6,7-3H]-estradiol to [3H]-water and [3H]-estradiol metabolites, the latter of which formed ring-substituted conjugates with nucleophiles like monothioglycerol and BSA. Tritium 76-78 tyrosinase Homo sapiens 29-39 6352447-6 1983 Both quinacrine (an inhibitor of phospholipase A2 activity) and indomethacin (an inhibitor of prostaglandin biosynthesis) abolished the action of prolactin on [3H]-thymidine incorporation into DNA. Tritium 160-162 phospholipase A2, group IB, pancreas Mus musculus 33-49 6311416-1 1983 Epiglycanin, the major glycoprotein of TA3-Ha mammary carcinoma ascites cells, was radiolabeled with tritium in the terminal D-galactose and 2-acetamido-2-deoxy-D-galactose residues. Tritium 101-108 mucin 21 Mus musculus 0-11 6307147-1 1983 An investigation of the structure and biosynthesis of alpha-galactosidase A (alpha-D-galactoside glycohydrolase, EC 3.2.1.22) and its N-linked oligosaccharide chains was undertaken by metabolic labeling of Chang liver cells with [2-3H]mannose, immunoprecipitation of the activity, and examination of the resulting immunoprecipitates. Tritium 231-234 galactosidase alpha Homo sapiens 54-75 6191193-4 1983 The concentration of immunologically detectable MAO B protein in the extracts was estimated from immunoprecipitation competition data by reference to a standard curve relating observed inhibition of immunoprecipitation to the concentration of catalytically active platelet MAO added (estimated from [3H]pargyline binding data). Tritium 300-302 monoamine oxidase B Homo sapiens 48-53 4067325-2 1985 [3H]Retinol bound to retinol-binding protein (RBP) was incubated for 24 h with the tissue sample, which was then extracted and analyzed by high-performance liquid chromatography. Tritium 1-3 retinol binding protein 4 Homo sapiens 46-49 2932448-10 1985 The phosphate ester must be located to C-2 of xylose/xylitol as the 1-3H radioactivity could be released by periodate oxidation when it was preceded by alkaline phosphatase treatment. Tritium 70-72 complement C2 Bos taurus 39-42 2935410-4 1985 [3H]Mesulergine bound in pig choroid plexus and in rat cortex (besides binding to 5-HT2 sites in rat cortex) to the 5-HT1C recognition site characterized by high affinity for metergoline, mesulergine, 5-HT and methergine and low affinity for (-)21-009, ICYP, 8-OH-DPAT and spiroperidol. Tritium 1-3 5-hydroxytryptamine receptor 2C Rattus norvegicus 116-122 4085545-1 1985 The cholecystokinin (CCK) antagonist, proglumide, administered chronically (41.0-53.5 mg/kg per day, 14 days) to rats via osmotic mini-pumps, produced a significant 13% increase in the number of [3H]spiperone labeled binding sites (Bmax) in the striatum. Tritium 196-198 cholecystokinin Rattus norvegicus 4-19 2994848-3 1985 Depletion of pyramidal cells and consequent severe decrease in [3H]CHA binding activity in the CA1 subfield were observed after transient ischemic insult. Tritium 64-66 carbonic anhydrase 1 Homo sapiens 95-98 21731704-7 2011 In contrast, the GlyT-2 inhibitor ALX1393 exhibited very weak for [3H]CHIBA-3007 binding. Tritium 67-69 solute carrier family 6 member 5 Rattus norvegicus 17-23 2936600-10 1985 The conversion rate of 17-OH-[3H]P5 to 17-OH-[3H]P4 and 11-deoxy-[3H] cortisol was higher in atrophic adrenal cells than in normal adrenal cells, but the conversion rate to [3H]DHEA, [3H]DHEA-S and [3H]delta 4-A was significantly lower in atrophic adrenal cells than in normal adrenal cells. Tritium 30-32 sulfotransferase family 2A member 1 Homo sapiens 187-193 2995978-2 1985 Low concentrations of [D-Ala2, D-Leu5]enkephalin selectively inhibit the mu1 binding of [3H]dihydromorphine, leaving mu2 sites, while low morphine concentrations eliminate the mu1 binding of [3H][D-Ala2, D-Leu5]enkephalin, leaving delta sites. Tritium 89-92 sodium voltage-gated channel alpha subunit 4 Rattus norvegicus 73-76 2995978-4 1985 The regional distributions of mu1 sites labeled with [3H]dihydromorphine were quite similar to those determined by using [3H][D-Ala2, D-Leu5]enkephalin. Tritium 54-57 sodium voltage-gated channel alpha subunit 4 Rattus norvegicus 30-33 6343748-5 1983 The use of purified HNMT in the Hm assay has allowed the inclusion of high specific activity tritiated S-adenosyl-L-methionine ([3H]SAME) and the development of a simplified solvent extraction product isolation procedure. Tritium 129-131 histamine N-methyltransferase Homo sapiens 20-24 6852262-1 1983 The existence of two progesterone receptor forms present in crude cytosol of chick oviduct has been demonstrated by photoaffinity labelling using [3H]R5020. Tritium 147-149 progesterone receptor Gallus gallus 21-42 6852262-4 1983 The peptide map obtained for chick oviduct cytosol progesterone receptor crosslinked with [3H]R5020 proved to be the sum of peptides obtained from partially purified preparations of forms A and B. Tritium 91-93 progesterone receptor Gallus gallus 51-72 4074717-4 1985 After preincubation of cells for 16 h in the presence or absence of glucose followed by a 60-min pulse of [3H]mannose, there was an 8-fold increase in labeled TSH + alpha but only a minimal change in free beta or total proteins. Tritium 107-109 glycoprotein hormones, alpha subunit Mus musculus 159-170 20881247-7 2010 Oatp2 seems to have some role in the influx of [(3)H]cortisol and [(3)H]corticosterone to the choroid plexus and the pituitary gland and other transporters, unlikely to be oatp2, may play a very minor role in the access of [(3)H]cortisol and [(3)H]corticosterone to the brain, as well as having a significant effect on [(3)H]glucocorticoid receptor accumulation in the pituitary gland. Tritium 48-52 solute carrier organic anion transporter family member 1B1 Homo sapiens 0-5 2997888-3 1985 DHEA-S and plasma DLF displaced [125I]digoxin from digoxin antibody, inhibited hog brain Na,K-ATPase and displaced [3H]ouabain from ATPase in a concentration dependent manner similar to digoxin. Tritium 116-118 sulfotransferase family 2A member 1 Homo sapiens 0-6 3899113-6 1985 These results indicate that [3H]HACBO-Gly binds selectively to enkephalinase. Tritium 29-31 membrane metallo-endopeptidase Rattus norvegicus 63-76 6302214-6 1983 Both glucosamine and mannose were components of M26 to M34 but [3H]fucose appeared to be associated mainly with M34. Tritium 64-66 olfactory receptor family 10 subfamily D member 1 Mus musculus 112-115 6403041-4 1983 Endothelial cells incubated with [3H]arachidonic acid for 24 h and then exposed to bradykinin for 3 min release 3H into the medium, approximately 65% of which co-chromatographs with 6-ketoprostaglandin F1 alpha and 3% with thromboxane B2. Tritium 33-36 kininogen 1 Bos taurus 83-93 6403041-4 1983 Endothelial cells incubated with [3H]arachidonic acid for 24 h and then exposed to bradykinin for 3 min release 3H into the medium, approximately 65% of which co-chromatographs with 6-ketoprostaglandin F1 alpha and 3% with thromboxane B2. Tritium 34-36 kininogen 1 Bos taurus 83-93 20980790-5 2010 An HSP90 inhibitor, geldanamycin (GA), significantly suppressed angiotensin II-induced [3H]leucine incorporation and atrial natriuretic factor expression in cardiac cells. Tritium 88-90 heat shock protein 90 alpha family class A member 1 Homo sapiens 3-8 6185485-2 1983 The relative capacities of the various nucleic acids to associate with pure [3H]vimentin were determined in competition experiments with 28 S rRNA from Ehrlich ascites tumor cells. Tritium 77-79 vimentin Mus musculus 80-88 3896152-6 1985 Antisera specific for LS-tetrasaccharide c and 3"-sialyllactose (NeuAc alpha 2-3Gal beta 1-4Glc) identify their corresponding 3H-labeled haptens released from the major meconium gangliosides 6"-LM1 and GM3, respectively. Tritium 126-128 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 Homo sapiens 84-90 6337503-7 1983 Insulin infusion at 0.05 U X kg-1 X h-1 by the peripheral and portal circulations reduced 3H-Ra to the normal range: 3.1 and 2.8 mg X kg-1 X min-1, respectively. Tritium 90-92 insulin Canis lupus familiaris 0-7 20489167-5 2010 In Npc1(-/-) hepatocytes, the incorporation of [3H]oleic acid and [3H]acetate into TG was decreased, but shunting of oleic acid- or acetate-derived [3H]carbons into cholesterol was increased. Tritium 48-50 NPC intracellular cholesterol transporter 1 Homo sapiens 3-7 6298780-4 1983 The capacity of GABA-modulin to decrease the number of sites that bind [3H]muscimol was completely abolished by phosphorylation of this protein with the cAMP-dependent protein kinase but not with the Ca2+-dependent enzyme. Tritium 72-74 KIT proto-oncogene receptor tyrosine kinase Rattus norvegicus 168-182 6424746-4 1983 [3H]-5-methoxyindoles were also formed from [3H]-melatonin ([3H]-aMT). Tritium 1-4 aminomethyltransferase, mitochondrial Melopsittacus undulatus 65-68 6847606-2 1983 The mucin was applied in low concentrations (1 microgram/track) to slab gels containing 0.5% agarose/2% (w/v) polyacrylamide, and bands were detected after electrophoresis by silver stain or by fluorography of 3H-labelled mucin. Tritium 210-212 solute carrier family 13 member 2 Rattus norvegicus 4-9 6872139-2 1983 In a previous study, we observed that the ratio of DE-I:DNA adducts to DE-II:DNA adducts in mouse epidermis, 24 h after application of [3H]5-MeC metabolites was 2.7 to 1. Tritium 136-138 chemokine (C-C motif) ligand 28 Mus musculus 141-144 6872139-3 1983 To investigate the basis for this observation we have now studies: (i) the formation of [3H]5-MeC in mouse epidermis in vivo at various time intervals from 0.33 to 24 h; (ii) the persistence of DE-I:DNA adducts and DE-II:DNA adducts in mouse epidermis at 4-48 h after application of [3H]5-MeC; and (iii) the reactions of DE-I and DE-II with calf thymus DNA in vitro. Tritium 89-91 chemokine (C-C motif) ligand 28 Mus musculus 94-97 6982898-1 1982 The regulation of ferritin synthesis by iron was examined in the reticulocytes of bullfrog tadpoles where the induction was 40- to 50-fold, increasing from 0.17 +/- 0.05% of total protein synthesis ([3H]leucine incorporation in cell suspension) to 7.4 +/- 1.6% following intraperitoneal injection of ferric ammonium citrate. Tritium 200-202 Fer2 Triticum aestivum 18-26 2994790-5 1985 Adrenaline (in the presence of rauwolscine), isoprenaline and the preferential beta 2-adrenoceptor agonist, procaterol, concentration-dependently increased the electrically evoked tritium overflow. Tritium 180-187 adrenoceptor beta 2 Homo sapiens 79-98 4009168-2 1985 After 10 days of treatment with the cholinesterase inhibitor diisopropyl fluorophosphate, [3H]ACh binding in the cortex, thalamus, striatum, and hypothalamus was decreased. Tritium 91-93 butyrylcholinesterase Rattus norvegicus 36-50 3840319-5 1985 NPY also reduced the TNS induced (2 Hz for 20 s), fractional [3H]NA release by 40% without affecting the contractile response. Tritium 62-64 pro-neuropeptide Y Cavia porcellus 0-3 2865278-3 1985 Arcuate POMC neurons were born very early in embryonic development, with peak heavy [3H]thymidine nuclear labelling occurring on embryonic day E12. Tritium 85-87 proopiomelanocortin Rattus norvegicus 8-12 19766183-14 2010 In conclusion, JNK inhibition by TAT-JBD treatment reduced neonatal HI brain damage with a therapeutic window of 3h and long-lasting anatomical and behavioral improvements. Tritium 113-115 mitogen-activated protein kinase 8 Rattus norvegicus 15-18 2485271-9 1985 After hydrolysis of bile obtained from homozygous and heterozygous Gunn rats with the enzyme beta-glucuronidase, the total amount of intact 25-(OH)-[3H]D3 recovered was found to be similar in the two groups of rats. Tritium 149-151 glucuronidase, beta Rattus norvegicus 93-111 2986881-7 1985 Recovery of [3H]AVP added to plasma averaged 86.6 (SD 6.1)% (n = 14). Tritium 13-15 arginine vasopressin Bos taurus 16-19 2992315-1 1985 A one-step procedure to detect cellular [3H]retinol and [3H]retinoic acid binding proteins (CRBP and CRABP) from rat testis cytosolic extract was devised. Tritium 41-43 retinol binding protein 1 Rattus norvegicus 92-96 6187905-5 1982 Rats challenged repeatedly with dinitrophenylated bovine serum albumin (DNP-BSA) had specific IgA antibody against DNP in the bile and feces as measured by a radioimmunoassay using [3H]-DNP-lysine. Tritium 182-184 albumin Rattus norvegicus 57-70 7107651-5 1982 Further studies by in vitro translation analysis using the reticulocyte lysate system and labeling with [3H]proline or [35S]methionine show definitive changes in patterns of protein synthesis and proline-rich protein mRNAs are highly elevated in treated animals. Tritium 105-107 proline rich protein 2-like 1 Rattus norvegicus 196-216 6292604-2 1982 The binding activity of three peptides was measured as their ability to inhibit the binding of [3H]neurotensin to rat and guinea-pig brain synaptic membranes. Tritium 96-98 neurotensin Rattus norvegicus 99-110 2992315-1 1985 A one-step procedure to detect cellular [3H]retinol and [3H]retinoic acid binding proteins (CRBP and CRABP) from rat testis cytosolic extract was devised. Tritium 57-59 retinol binding protein 1 Rattus norvegicus 92-96 20170649-9 2010 The preferential alpha(1B)-adrenoceptor antagonist, AH11110A, the alpha2-adrenoceptor agonist, RWJ52353, and the adrenergic neurotoxin DSP-4 likewise affected [3H]MPP+ transport, in an OCT-subtype and species-dependent manner. Tritium 160-162 plexin A2 Homo sapiens 185-188 2990164-1 1985 Dopamine reduces the affinity and increases the number of [3H]-neurotensin binding sites in the subcortical limbic forebrain of the rat. Tritium 59-61 neurotensin Rattus norvegicus 63-74 7126543-2 1982 Using reverse-phase high-performance liquid chromatographic (HPLC) techniques, we have shown that biologically active bovine PTH (bPTH) internally labeled with [3H]tyrosine is, like 125I-labeled bPTH, rapidly metabolized by isolated rat Kupffer cells in vitro to multiple COOH-terminal fragments that are chemically identical with those previously found in plasma after metabolism in vivo. Tritium 161-163 parathyroid hormone Bos taurus 125-128 7044761-5 1982 The labeled protein, as evidenced by a peak in 3H or 14C disintegrations per min, also comigrated with renin activity in isoelectric focusing gels. Tritium 47-49 renin Canis lupus familiaris 103-108 20170649-10 2010 In conclusion, D24, other isocyanine congeners and chemically-related adrenergic agents inhibit OCT-mediated [3H]MPP+ transport, and all drugs display significant activity at alpha1- and alpha2-adrenoceptor subtypes, expanding previous reports of promiscuity between pharmacophores recognising alpha-adrenoceptors and OCTs. Tritium 110-112 plexin A2 Homo sapiens 96-99 3971913-3 1985 Incubation of the oviduct cytosol containing [3H]PRc at either 23 or 0 C with 0.3 M KCl or 5-10 mM ATP for 1-4 h resulted in the appearance of a slower migrating form with a sedimentation rate of approximately 4S and a complete and concomitant disappearance of the 8S PR form. Tritium 46-48 progesterone receptor Gallus gallus 49-51 7097729-1 1982 The affinity, relative to cortisol (1), of 17 alpha- and 21-esters and 17 alpha,21-diesters of cortisol for the glucocorticoid receptor of rat thymus cytosol was determined by a competitive binding assay which used [3H]dexamethasone. Tritium 216-218 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 112-135 20045082-6 2010 Injection of IL2 alone significantly increased resistance to venous return and arterial resistance at 3h post injections. Tritium 102-104 interleukin 2 Rattus norvegicus 13-16 6918355-1 1982 The effect of three elastase inhibitors (MeO-Suc-Ala-Ala-Pro-Val-CH2Cl, eglin c and alpha 1-proteinase inhibitor) on the hydrolysis of [3H]elastin was determined. Tritium 136-138 elastin Homo sapiens 139-146 3161932-4 1985 The rate constants of dissociation (k-1, sec-1) were 3.6 X 10(-5) for 3H-R5020, 21.3 X 10(-5) for 3H-progesterone and 17.8 X 10(-5) for 3H-CPA. Tritium 70-72 secretory blood group 1, pseudogene Homo sapiens 41-46 4009066-6 1985 Inactivation of lecithin: cholesterol acyltransferase (LCAT) and heating the HDL2 for 60 min at 56 degrees C accelerated transfer and exchange of [3H]cholesteryl esters. Tritium 147-149 lecithin-cholesterol acyltransferase Homo sapiens 16-53 4009066-6 1985 Inactivation of lecithin: cholesterol acyltransferase (LCAT) and heating the HDL2 for 60 min at 56 degrees C accelerated transfer and exchange of [3H]cholesteryl esters. Tritium 147-149 lecithin-cholesterol acyltransferase Homo sapiens 55-59 3973592-2 1985 When [3H]Met was administered, both AdoMet and phospholipid pools were labeled. Tritium 6-8 methionine adenosyltransferase 1A Rattus norvegicus 36-42 3155738-10 1985 Each milligram of isolated GP IIb-IIIa complex bound 0.30 mg of [3H]Triton X-100, indicating that the glycoprotein complex contained limited hydrophobic domains. Tritium 64-68 integrin subunit alpha 2b Homo sapiens 27-33 6277413-2 1982 Serum samples collected after ALP infusions inhibited 3H-thymidine incorporation by human leukemic cells to an extent that correlated to the dose of ALP administered. Tritium 54-56 ATHS Homo sapiens 30-33 6277413-2 1982 Serum samples collected after ALP infusions inhibited 3H-thymidine incorporation by human leukemic cells to an extent that correlated to the dose of ALP administered. Tritium 54-56 ATHS Homo sapiens 149-152 7057266-6 1982 [3H]K-1 or [3H]epoxide could not be detected as metabolites of [3H]epoxide diol indicating that the activity of epoxide diol was probably not due to its conversion to K-1 hydroquinone, since any [3H]hydroquinone formed would be oxidized in air to [3H]K-1 during analysis. Tritium 1-3 keratin 1 Rattus norvegicus 4-7 6277664-7 1982 In contrast atenolol, a beta-antagonist devoid of "quinidine-like" activity was ineffective at 4 degree C. Furthermore, procaine, and quinidine itself were potent competitors of [3H]dihydroalprenolol binding at 4 degree C. Thus the specificity of [3H]dihydroalprenolol binding to rat heart membranes at 4 degree C appears to be directed non-stereoselectively at that portion of the competing molecule which recognized "quinidine-like" as opposed to adrenergic activity. Tritium 179-181 amyloid beta precursor protein Rattus norvegicus 22-28 3881988-2 1985 [3H-PheB1]insulin ([3H]insulin) was infused as a tracer from time 0 at a constant rate. Tritium 1-3 insulin Canis lupus familiaris 10-17 6128265-2 1982 In Xenopus laevis embryos, the 26 S polyadenylated mRNA, coding for the myosin heavy chain, shows a maximum relative concentration as measured by the 3H-poly(U)-hybridization technique, at the neural-plate stage (st. 12.5 of Nieuwkoop and Faber, [NF]) only in the dorsal (ectodermal and mesodermal) region, i.e. shortly before the morphological appearance of the somites. Tritium 150-152 myhc Xenopus laevis 72-90 19864054-2 2010 To get the TiO(2)/Sep nanoparticle, the nanocrystalline TiO(2) anatase phase on sepiolite was obtained using a sufficient thermal treatment by gradually increasing the temperature from 300, 400 and 500 degrees C for 3h. Tritium 216-218 plexin B1 Homo sapiens 18-21 6290351-2 1982 Specific binding of [3H]-oxytocin was rapid, reversible, and saturable. Tritium 21-23 oxytocin/neurophysin I prepropeptide Homo sapiens 25-33 6290351-4 1982 On fractionation of the myometrium by differential and discontinuous gradient centrifugation, [3H]-oxytocin binding showed a fractionation pattern which was similar to that for plasma membrane markers, but different from those for endoplasmic reticulum or mitochondrial markers. Tritium 95-97 oxytocin/neurophysin I prepropeptide Homo sapiens 99-107 3881988-2 1985 [3H-PheB1]insulin ([3H]insulin) was infused as a tracer from time 0 at a constant rate. Tritium 20-22 insulin Canis lupus familiaris 10-17 3881988-14 1985 It is concluded that 1) insulin turnover rates can be measured accurately using [3H]insulin as a tracer and 2) insulin kinetics are nonlinear. Tritium 81-83 insulin Canis lupus familiaris 24-31 3881988-14 1985 It is concluded that 1) insulin turnover rates can be measured accurately using [3H]insulin as a tracer and 2) insulin kinetics are nonlinear. Tritium 81-83 insulin Canis lupus familiaris 84-91 2578570-6 1985 Net incorporation of [3H]thymidine terminated at about 30 min after infection of pR386-containing cells with unf- phage at 30 degrees C. Alkaline sucrose gradient studies of the intracellular pools of replicative DNA in unf-infected plasmid pR386-containing cells indicated that this DNA is not detectably nickel or cleaved at the time that DNA synthesis aborts. Tritium 22-24 Alc inhibitor of host transcription Escherichia phage T4 109-112 3968538-5 1985 [3H]Mannose-labelled E1, E2, GP59(E1) and GP43(E2) were digested with Pronase and the glycopeptides separated by gel filtration. Tritium 1-3 cystatin 12, pseudogene Homo sapiens 42-50 20978326-6 2010 In TT cells, PTTG1 silencing did not completely block DNA synthesis, but significantly reduced [3H]Thymidine incorporation (~50%; p<0.01) for up to 3 days. Tritium 96-98 PTTG1 regulator of sister chromatid separation, securin Homo sapiens 13-18 2983359-2 1985 Specific binding of 5 nmol/l [3H]PIA at 37 degrees C was rapid, reversible and dependent on cell concentration and the presence of adenosine deaminase. Tritium 30-32 adenosine deaminase Rattus norvegicus 131-150 6517935-4 1984 Affinity labeling of GR with [3H]-dexamethasone mesylate showed association of the radioactivity with a peptide of 90,000 molecular weight. Tritium 30-32 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 21-23 16662157-8 1982 Analyses of incorporation of (3)H into the pro-2R and pro-2S hydrogens of glycolate, in the presence and absence of alpha-hydroxy-2-pyridinemethanesulfonic acid, suggest an alternative source of glycolate. Tritium 29-33 profilin-2 Nicotiana tabacum 43-48 7333283-8 1981 The steric distribution of the tritium in the samples of malate, as shown by the action of fumarase, revealed that D-(1R)- and D-(1S)-(1-2H1)[1-3H]fructose 6-phosphate was converted into (R)- and (S)-(2H1)[3H]acetyl-phosphate, respectively. Tritium 31-38 fumarate hydratase Homo sapiens 91-99 19681653-2 2010 In the frontal cortex, B(max) of [3H]SCH 23390 binding sites was found to be increased in T10 losers and decreased in T20 losers when compared to the control mice. Tritium 34-36 transport and golgi organization 2 Mus musculus 90-93 7033900-3 1981 3H-labelled kappa-elastin was administered iv and percutaneously and its elimination and organ distribution determined. Tritium 0-2 elastin Rattus norvegicus 18-25 6542056-0 1984 Penetration of [3H]T-2 toxin through excised human and guinea-pig skin during exposure to [3H]T-2 toxin adsorbed to corn dust. Tritium 16-18 solute carrier family 25 member 5 Homo sapiens 94-97 6542056-0 1984 Penetration of [3H]T-2 toxin through excised human and guinea-pig skin during exposure to [3H]T-2 toxin adsorbed to corn dust. Tritium 91-93 solute carrier family 25 member 5 Homo sapiens 19-22 6542056-0 1984 Penetration of [3H]T-2 toxin through excised human and guinea-pig skin during exposure to [3H]T-2 toxin adsorbed to corn dust. Tritium 91-93 solute carrier family 25 member 5 Homo sapiens 94-97 6542056-2 1984 To simulate the conditions which occur when agricultural workers are exposed to corn dust contaminated with T-2 toxin, the epidermal surface of each skin preparation was dosed with [3H]T-2 toxin adsorbed onto corn dust. Tritium 182-184 solute carrier family 25 member 5 Homo sapiens 185-188 6542056-3 1984 The applied dose was 3.27 to 4.75 mg of corn dust containing 18.2 ppm [3H]T-2 toxin. Tritium 71-73 solute carrier family 25 member 5 Homo sapiens 74-77 19681653-3 2010 In the striatum, T10 and T20 winners had reduced values of [3H]SCH 23390 binding sites than the ones in the control mice. Tritium 60-62 transport and golgi organization 2 Mus musculus 17-20 6947265-6 1981 When MHC [3H]mRNPs were encapsulated into liposomes and subsequently delivered to myoblasts and myotubes, the mRNPs were taken up by the cells at both stages of differentiation. Tritium 10-12 major histocompatibility complex, class I, C Homo sapiens 5-8 6947265-7 1981 However, the MHC [3H]mRNPs taken up by the myoblasts remained as free cytoplasmic particles (80-120S), whereas in myotubes the incorporated mRNP RA was associated with polysomes. Tritium 18-20 major histocompatibility complex, class I, C Homo sapiens 13-16 7264664-2 1981 [3H]Pargyline was bound to MAO A in a crude mitochondrial fraction from the placental trophoblast of a male newborn and to MAO B in blood platelets from the umbilical vein of the same newborn. Tritium 1-3 monoamine oxidase B Homo sapiens 123-128 7264664-3 1981 [3H]Pargyline was also bound to MAO A and B in a crude mitochondrial fraction from cultured skin fibroblasts of a male adult and to MAO B in blood platelets from the same individual. Tritium 1-3 monoamine oxidase B Homo sapiens 132-137 7264664-5 1981 For all tissues, SDS-PAGE of [3H]pargyline-bound samples revealed a labeled protein band of apparent molecular weight 63,000 for MAO A and 60,000 for MAO B. Tritium 30-32 monoamine oxidase B Homo sapiens 150-155 7227306-4 1981 The two peaks in the 10,000 Mr region had the electrophoretic mobility of [3H]bovine proparathyroid hormone (ProPTH) and [3H]bovine PTH (1--84), respectively. Tritium 75-77 parathyroid hormone Bos taurus 112-115 7227306-5 1981 Immunoreactive PTH was only detected in a peak comigrating with [3H]PTH-(1--84). Tritium 65-67 parathyroid hormone Bos taurus 15-18 7227306-5 1981 Immunoreactive PTH was only detected in a peak comigrating with [3H]PTH-(1--84). Tritium 65-67 parathyroid hormone Bos taurus 68-71 6568228-6 1984 However, macrophages cultured in 10% human serum and in contact with purified 3H-elastin degraded 4.7 micrograms elastin/10(6) cells per 24 h, as compared to less than 1 microgram/10(6) cells/24 h for neutrophils. Tritium 78-80 elastin Homo sapiens 81-88 6568228-6 1984 However, macrophages cultured in 10% human serum and in contact with purified 3H-elastin degraded 4.7 micrograms elastin/10(6) cells per 24 h, as compared to less than 1 microgram/10(6) cells/24 h for neutrophils. Tritium 78-80 elastin Homo sapiens 113-120 6434531-6 1984 Their Ki values against [3H]GEMSA binding are similar to their Ki values against enkephalin convertase activity. Tritium 25-27 carboxypeptidase E Bos taurus 81-102 6434531-9 1984 [3H] GEMSA binding activity is found only in fractions containing enkephalin convertase during enzyme purification from bovine pituitary by L-arginine affinity chromatography. Tritium 1-3 carboxypeptidase E Bos taurus 66-87 6434531-10 1984 These data confirm that [3H]GEMSA binds only to enkephalin convertase in crude homogenates under our assay conditions. Tritium 25-27 carboxypeptidase E Bos taurus 48-69 6434535-6 1984 Within 5 min after stimulation, 14%, 7%, and 7% of the total 1-O-[3H]alkyl-2-arachidonoyl-GPC in the cells had been converted to 1-O-[3H]alkyl-2-acetyl-GPC (platelet-activating factor), 1-O-[3H]alkyl-2-lyso-GPC, and 3H-labeled neutral lipid, respectively. Tritium 66-68 glycophorin C (Gerbich blood group) Homo sapiens 90-93 19625610-7 2009 Furthermore, EGF-induced increase of cell cycle proteins expression level and [3H]thymidine incorporation was blocked by MMP inhibitor. Tritium 79-81 epidermal growth factor Mus musculus 13-16 6501025-1 1984 The aim of this study was, first, to examine pressure effects of platelet-activating factor (PAF) on pulmonary vasculature and bronchi in isolated perfused and ventilated rat lungs and, second, to investigate pulmonary uptake of tritium-labeled PAF injected into the pulmonary artery. Tritium 229-236 PCNA clamp associated factor Rattus norvegicus 245-248 6501025-6 1984 Comparable changes in tritium outflow rates (19.2 +/- 3.9, 38.2 +/- 7.2%) occurred when tracer amounts of labeled PAF were injected, but, in KRS with 2.0 and 4.0% albumin, tritium outflow was significantly lower (52.8 +/- 8.0 and 60.8 +/- 11.8%, respectively). Tritium 22-29 PCNA clamp associated factor Rattus norvegicus 114-117 6541946-1 1984 Apical membrane vesicles isolated from a continuous renal cell line, LLC-PK1, catalyze electrogenic Na+-stimulated hexose transport and Na+-dependent binding of 3H-labeled 1-[2-(beta-D-glucopyranosyloxy)-4, 6-dihydroxyphenyl]-3-(4-hydroxyphenyl)-1-propanone [( 3H]phlorizin), a competitive ligand of this transport system. Tritium 161-163 prokineticin 1 Homo sapiens 73-76 6260280-2 1981 The light microscopic localization of the neurotensin receptor has been carried out by the autoradiographic localization of [3H]neurotensin binding sites in slide-mounted tissue sections. Tritium 125-127 neurotensin Rattus norvegicus 42-53 6260280-2 1981 The light microscopic localization of the neurotensin receptor has been carried out by the autoradiographic localization of [3H]neurotensin binding sites in slide-mounted tissue sections. Tritium 125-127 neurotensin Rattus norvegicus 128-139 19685929-6 2009 At acidic pH, protons and [3H]vesamicol compete for binding to VAChT. Tritium 27-29 solute carrier family 18 member A3 Homo sapiens 63-68 7458089-6 1981 Autoradiography after instillation of [3H]5-HT into the trachea showed localization of developed silver grains over both alveolar epithelium and endothelium; the grain density/mu2 was greatest in endothelial cells, and significantly exceeded the predicted value based on uniform distribution only for this compartment. Tritium 39-41 adaptor related protein complex 2 subunit mu 1 Rattus norvegicus 176-179 6117356-2 1981 Local injection of somatostatin, i.e., without any measurable systemic effect, resulted in a 75% reduction of cell proliferation as measured by [3H]thymidine incorporation and ornithine decarboxylase activities. Tritium 145-147 somatostatin Homo sapiens 19-31 6541061-8 1984 The control protein [3H] bovine serum albumin (BSA) was cleared from the blood with a time course identical to that of photolabeled ABP (t1/2"s 4.69 +/- 0.09 h and 13.56 +/- 1.03 h). Tritium 21-23 albumin Rattus norvegicus 32-45 6235235-4 1984 Deglycosylation of the HS-PG with polyhydrogen fluoride resulted in a 3H-core protein with Mr congruent to 2.4 X 10(5). Tritium 70-72 CD44 molecule (Indian blood group) Homo sapiens 23-28 19691347-2 2009 The activities of the two most potent CypA inhibitors (3h and 3i) are 2.59 and 1.52 nM, respectively, which are about 16 and 27 times more potent than that of cyclosporin A. Tritium 55-57 peptidylprolyl isomerase A Homo sapiens 38-42 6743684-5 1984 The osteoarthritic cartilage synthesized and accumulated up to 5-fold more [3H]fibronectin than disease-free cartilage. Tritium 76-78 fibronectin 1 Canis lupus familiaris 79-90 6611185-3 1984 Our studies on the proliferation of a murine T-cell lymphoma (EL-4) showed that the treatment of the ascitic fluid (from the peritoneum of EL-4 bearing mice) with carbonyl iron resulted in a depletion of phagocytes concomitant with a significant increase of [3H] thymidine uptake by EL-4 cells. Tritium 259-261 epilepsy 4 Mus musculus 62-66 6611185-6 1984 Also, conditioned media obtained from in vitro liquid cultures of EL-4 cells and L 1210 cells (B-lymphoma) produced a remarkable inhibition of the in vitro cloning capacity and [3H] thymidine uptake by EL-4 cells. Tritium 178-180 epilepsy 4 Mus musculus 66-70 7215349-6 1981 The content of [3H]NoHOQnO-specific binding sites is related to the cytochrome b content in the particles. Tritium 16-18 mitochondrially encoded cytochrome b Homo sapiens 68-80 7236214-9 1980 A detailed description of the synthesis of the 2H- and 3H-substituted ethanol compounds and the analysis of these has been deposited as Supplementary Publication SUP 50110 (5 pages) at the British Library Lending Division, Boston Spa, Wetherby, W. Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. Tritium 55-57 surfactant protein A2 Homo sapiens 230-233 19414624-6 2009 The transport of [(3)H]digoxin, the P-glycoprotein (P-gp) substrate, after treatment with 100 nM 1,25(OH)(2)D(3) for 3 days revealed a higher apparent permeability (P(app)) value in the basal (B)-to-apical (A) direction over that of vehicle treatment (15.1 +/- 0.53 x 10(-6) versus 11.8 +/- 0.58 x 10(-6) cm/s; P < 0.05), whereas the P(app) in the A-to-B direction was unchanged; the efflux ratio was increased (from 5.8 to 8.0). Tritium 19-22 phosphoglycolate phosphatase Homo sapiens 52-56 7435561-0 1980 Accumulation of [14C]urate and [3H]PAH in isolated proximal tubular segments of the rabbit kidney. Tritium 32-34 phenylalanine-4-hydroxylase Oryctolagus cuniculus 35-38 7435561-3 1980 In these segments the uptake of [3H]PAH was 22.7 +/- 4.6 (n = 19), 133 +/- 18.9 (n = 24), and 66.2 +/- 15.3 (n =0). Tritium 33-35 phenylalanine-4-hydroxylase Oryctolagus cuniculus 36-39 7435561-4 1980 The accumulation of [14C]urate was saturated at 2 x 10(-4) M in the medium, and that of [3H]PAH at 7 x 10(-5) M in the medium. Tritium 89-91 phenylalanine-4-hydroxylase Oryctolagus cuniculus 92-95 6450045-2 1980 Within 1 d after the addition of 0.1, 2, or 10 microgram retinyl acetate per ml of medium, there was a concentration-dependent increase in the uptake of [3H]glucosamine and [14C]serine into both the total mucous glycoprotein and the principal purified mucin fraction eluted from a DEAE-Sephacel column with 0.2 M NaCl. Tritium 154-156 solute carrier family 13 member 2 Rattus norvegicus 252-257 6611185-6 1984 Also, conditioned media obtained from in vitro liquid cultures of EL-4 cells and L 1210 cells (B-lymphoma) produced a remarkable inhibition of the in vitro cloning capacity and [3H] thymidine uptake by EL-4 cells. Tritium 178-180 epilepsy 4 Mus musculus 202-206 6744039-1 1984 The characteristics of [3H]neurotensin binding were studied using membranes prepared from the rat brain. Tritium 24-26 neurotensin Rattus norvegicus 27-38 6744039-2 1984 Binding of [3H]neurotensin was found to be specific, saturable and reversible. Tritium 12-14 neurotensin Rattus norvegicus 15-26 6744039-4 1984 The specific [3H]neurotensin binding increased linearly with protein concentration and was dependent on the pH and the temperature of the incubation medium. Tritium 14-16 neurotensin Rattus norvegicus 17-28 6744039-8 1984 A Hill transformation of the competitive inhibition of specific [3H]neurotensin binding by increasing concentrations of unlabelled peptide yielded a slope not significantly different from unity. Tritium 65-67 neurotensin Rattus norvegicus 68-79 6744039-9 1984 Neurotensin 1-13 and various neurotensin analogues were tested for their ability to compete with [3H]neurotensin for its binding site. Tritium 98-100 neurotensin Rattus norvegicus 0-11 6744039-9 1984 Neurotensin 1-13 and various neurotensin analogues were tested for their ability to compete with [3H]neurotensin for its binding site. Tritium 98-100 neurotensin Rattus norvegicus 101-112 6744039-10 1984 Neurotensin 1-13, neurotensin 8-13 and the amphibian skin peptide xenopsin were equipotent and strongly inhibited the specific binding of [3H]neurotensin. Tritium 139-141 neurotensin Rattus norvegicus 0-11 6744039-10 1984 Neurotensin 1-13, neurotensin 8-13 and the amphibian skin peptide xenopsin were equipotent and strongly inhibited the specific binding of [3H]neurotensin. Tritium 139-141 neurotensin Rattus norvegicus 18-29 6744039-10 1984 Neurotensin 1-13, neurotensin 8-13 and the amphibian skin peptide xenopsin were equipotent and strongly inhibited the specific binding of [3H]neurotensin. Tritium 139-141 neurotensin Rattus norvegicus 142-153 6744039-12 1984 Physiological concentrations of sodium chloride inhibited specific [3H]neurotensin binding, whereas divalent cations and guanyl nucleotides did not produce a significant change in either the equilibrium dissociation constant or the total number of binding sites. Tritium 68-70 neurotensin Rattus norvegicus 71-82 6744039-15 1984 In general, there was less variation between different brain regions in the number of [3H]neurotensin binding sites than in the content of neurotensin-like immunoreactivity. Tritium 87-89 neurotensin Rattus norvegicus 90-101 6727566-6 1984 The percent of 3H-PRL released did not differ between neonatally hormone treated and control animals, suggesting that neonatal treatment affected mechanisms that regulate PRL synthesis but not those that regulate release. Tritium 15-17 prolactin Mus musculus 18-21 7419623-1 1980 A tritium compound with low molecular weight and diameter, N-succinimidyl-[2,3-3H]-propionate (3H-NSP), was used to label histones and nonhistone proteins from calf thymus, and nuclear and total salivary gland proteins from larvae of the midge Chironomus thummi. Tritium 2-9 NSP Bos taurus 98-101 19373748-5 2009 The glucose transporter GLUT4 prepared for [3H]-glucosamine uptake showed a concentration-dependent competition between glucose and glucosamine uptake. Tritium 44-46 solute carrier family 2 member 4 Rattus norvegicus 24-29 7456368-1 1980 In blood plasma of men activity of lecithin-cholesterol acyl transferase /LCAT/ was elevated when the cholesterol concentration was increased in the fraction of high density lipoproteins /HDL/; the enzymatic activity was evaluated by the rate of 3H-cholesterol esterification in endogenous HDL. Tritium 246-248 lecithin-cholesterol acyltransferase Homo sapiens 74-78 6990984-2 1980 When either [5,6-3H]arachidonic acid or [5,6-3H]PGH2 is converted into PGI2 by swine aortic microsomes, the tritium at C-6 is lost to the medium. Tritium 108-115 complement C6 Sus scrofa 119-122 6323630-2 1984 The binding of [3H]NDP to adult heart and brain was kinetically very similar, with the former displaying a KD of 0.28 +/- 0.02 nM and a Bmax of 138 +/- 17 fmol/mg protein, and the latter a KD of 0.30 +/- 0.02 nM and a Bmax of 160 +/- 12 fmol/mg protein. Tritium 16-18 NDP, norrin cystine knot growth factor Gallus gallus 19-22 6323630-3 1984 The binding site in both brain and heart was highly specific for dihydropyridine calcium antagonists, such as nifedipine, nimodipine, and nisoldipine, since these drugs were several orders of magnitude more potent as inhibitors of [3H]NDP binding than verapamil, methoxyverapamil, or diltiazem. Tritium 232-234 NDP, norrin cystine knot growth factor Gallus gallus 235-238 6323630-4 1984 The developmental appearance of [3H]NDP binding sites in brain was rather gradual, with adult levels being attained just prior to birth. Tritium 33-35 NDP, norrin cystine knot growth factor Gallus gallus 36-39 6323630-6 1984 The acquisition of [3H]NDP binding sites in chick brain roughly paralleled the onset of neuronal maturation and functional activity. Tritium 20-22 NDP, norrin cystine knot growth factor Gallus gallus 23-26 7387399-5 1980 Protein synthesis measured by the incorporation of [3H]-leucine in the cell proteins begins to be inhibited after 30 min and is completely blocked after 2 h for a ricin concentration of 0.3 microgram/ml. Tritium 52-54 ricin Ricinus communis 163-168 19360096-10 2009 Furthermore, the AAD mutants exhibited altered expression levels of the DEG-3 subfamily nAChR genes Hco-mptl-1, Hco-des-2H and Hco-deg-3H as quantified by real-time PCR. Tritium 135-137 Acetylcholine receptor subunit alpha-type deg-3 Caenorhabditis elegans 72-77 6985917-7 1980 At 0.25-0.5 U/ml of thrombin, phosphorylation preceded [3H]serotonin release with the time for half-maximal release being 26.0 +/- 1.3 s SE (n = 3) and the time for half-maximal phosphorylation being 12.3 +/- 1.3 s SE (n = 3) for P7P and 3.7 +/- 0.17 s SE (n = 3) for P9P. Tritium 56-58 prothrombin Oryctolagus cuniculus 20-28 6696920-5 1984 Evaluation of tritium release from [5-3H]dCyd proved to be the most convenient method that has been described so far to measure thymidylate synthase activity and to follow the inhibitory effects of thymidylate synthase inhibitors in intact L1210 cells, since this method is rapid and very sensitive, and since it proved superior to the evaluation of tritium release from [5-3H]dUrd because it circumvents possible interactions of the inhibitors with thymidine kinase activity. Tritium 14-21 thymidylate synthase Mus musculus 128-148 6696920-5 1984 Evaluation of tritium release from [5-3H]dCyd proved to be the most convenient method that has been described so far to measure thymidylate synthase activity and to follow the inhibitory effects of thymidylate synthase inhibitors in intact L1210 cells, since this method is rapid and very sensitive, and since it proved superior to the evaluation of tritium release from [5-3H]dUrd because it circumvents possible interactions of the inhibitors with thymidine kinase activity. Tritium 14-21 thymidylate synthase Mus musculus 198-218 6477602-2 1984 The 3H-labeled product was purified by DE 52 column chromatography followed by Cellulofine GCL 25 column chromatography. Tritium 4-6 germ cell-less 2, spermatogenesis associated Homo sapiens 91-94 6542769-4 1984 [3H]-Retinol-RBP (10(-6) M) was added to the medium for 1-24 h, after which epidermis and dermis were split and separately extracted with hexane after saponification. Tritium 1-3 retinol binding protein 4 Homo sapiens 13-16 6778604-1 1980 Cultured cells of rat bladder transitional cell carcinoma line AY-27, in suspension, were assayed for galactosyl transferase (GT) by measurement of the transfer of [3H]galactose from uridine diphosphate-[3H]galactose to desialylated ovine submaxillary mucin (OSM-NANA). Tritium 165-167 glycoprotein alpha-galactosyltransferase 1 Rattus norvegicus 102-124 19208517-8 2009 RESULTS: 3H-TdR incorporation increased significantly in LX-2 cells co-cultured with QSG7701-HBx cells compared to those cultured with QSG7701-pcDNA3 and QSG7701 (non-tumorigenic human liver cell line). Tritium 9-11 X protein Hepatitis B virus 93-96 227794-3 1979 The subpopulation which incorporated [3H]thymidine after exposure to virus consisted mainly of cells which formed rosettes with EAC. Tritium 38-40 CYLD lysine 63 deubiquitinase Homo sapiens 128-131 531082-0 1979 The fate of tritium labeled GM1 ganglioside injected in mice. Tritium 12-19 coenzyme Q10A Mus musculus 28-31 218622-3 1979 CRP modified with oPDM retains [3H]cAMP binding activity but loses [3H]d(I-C)n binding activity. Tritium 32-34 catabolite gene activator protein Escherichia coli 0-3 218622-3 1979 CRP modified with oPDM retains [3H]cAMP binding activity but loses [3H]d(I-C)n binding activity. Tritium 68-70 catabolite gene activator protein Escherichia coli 0-3 6322794-2 1984 Binding of (3H)-ouabain and ouabain-induced inhibition of the sodium pump and of the (Na+ + K+)-ATPase have been characterized in cultured cardiac muscle and non muscle cells, as well as in cardiac cell membranes--all obtained from chick embryos. Tritium 12-14 ATPase Na+/K+ transporting subunit alpha 1 Gallus gallus 86-102 6421635-8 1984 Digestion of the [3H]alpha-tocopherol--lipoxygenase complex with proteolytic enzymes showed that most of the radioactivity is incorporated into one peptide. Tritium 18-20 linoleate 9S-lipoxygenase-4 Glycine max 39-51 6610092-6 1984 The synthesis de novo and shedding of IAP by THP-1 were demonstrated by the immunoprecipitation of radioactive IAP in the culture fluids of [3H]leucine-labeled cells. Tritium 141-143 magnesium transporter 1 Homo sapiens 38-41 6610092-6 1984 The synthesis de novo and shedding of IAP by THP-1 were demonstrated by the immunoprecipitation of radioactive IAP in the culture fluids of [3H]leucine-labeled cells. Tritium 141-143 magnesium transporter 1 Homo sapiens 111-114 18649995-8 2008 Pretreatment of HUVEC with a p21, but not p27, antisense oligonucleotide reversed the DPTH-N10-induced inhibition of [3H]thymidine incorporation into HUVEC. Tritium 118-120 H3 histone pseudogene 16 Homo sapiens 29-32 6320181-6 1984 If, for example, the ratio of eIF-2 X GEF to total (phosphorylated and unphosphorylated) eIF-2 X [3H]GDP was 0.25, the exchange was found to be maximally inhibited when the proportion of eIF-2(alpha P) X [3H]GDP in hte mixture reached 25%. Tritium 98-100 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 38-41 6320181-6 1984 If, for example, the ratio of eIF-2 X GEF to total (phosphorylated and unphosphorylated) eIF-2 X [3H]GDP was 0.25, the exchange was found to be maximally inhibited when the proportion of eIF-2(alpha P) X [3H]GDP in hte mixture reached 25%. Tritium 205-207 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 38-41 6320181-10 1984 Incubation of eIF-2 X GEF with excess [3H]GDP leads to the formation of eIF-2 X [3H] GDP and free GEF and, if eIF-2(alpha 32P) X GDP is also present, all of the GEF is converted to eIF-2(alpha 32P) X GEF. Tritium 39-41 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 22-25 6320181-10 1984 Incubation of eIF-2 X GEF with excess [3H]GDP leads to the formation of eIF-2 X [3H] GDP and free GEF and, if eIF-2(alpha 32P) X GDP is also present, all of the GEF is converted to eIF-2(alpha 32P) X GEF. Tritium 39-41 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 98-101 6320181-10 1984 Incubation of eIF-2 X GEF with excess [3H]GDP leads to the formation of eIF-2 X [3H] GDP and free GEF and, if eIF-2(alpha 32P) X GDP is also present, all of the GEF is converted to eIF-2(alpha 32P) X GEF. Tritium 39-41 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 98-101 6320181-10 1984 Incubation of eIF-2 X GEF with excess [3H]GDP leads to the formation of eIF-2 X [3H] GDP and free GEF and, if eIF-2(alpha 32P) X GDP is also present, all of the GEF is converted to eIF-2(alpha 32P) X GEF. Tritium 81-83 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 22-25 86511-5 1979 Only when anti-AFP antiserum was the complexing protein and AFP, either crude or purified, was the binding protein did a substantial [3H]-oestradiol precipitate form. Tritium 134-136 alpha fetoprotein Mus musculus 15-18 86511-5 1979 Only when anti-AFP antiserum was the complexing protein and AFP, either crude or purified, was the binding protein did a substantial [3H]-oestradiol precipitate form. Tritium 134-136 alpha fetoprotein Mus musculus 60-63 639474-4 1978 Recycling of the 3H was minimized by using glutamate labelled at the C-2 position. Tritium 17-19 complement component 2 (within H-2S) Mus musculus 69-72 18331289-3 2008 In cells transfected with either 0.5 microg/mL or 3 microg/mL of alpha-Syn and 1 microg/mL of NET DNA, treatment with cytochalasin D, an actin depolymerizing agent, caused a dose-dependent decrease and increase, respectively, in [3H]-NE uptake. Tritium 230-232 synuclein alpha Homo sapiens 65-74 206750-5 1978 Met- and leu-enkephalin (10(-7)-10(-5) M) decreased the high potassium induced [3H]-norepinephrine release from vas deferens, while substance P (10(-6) M) significantly increased it. Tritium 80-82 prodynorphin Mus musculus 9-23 6201003-1 1984 Adult rats were examined for the time course of inactivation of antigenic determinants of tritium-labeled bovine serum albumin (BSA) or chicken ovalbumin administered intragastrically. Tritium 90-97 albumin Rattus norvegicus 113-126 6606427-4 1983 The glucocorticoid triamcinolone acetonide specifically inhibits both these androgen mediated events without altering the equilibrium dissociation constant (Kd) of the androgen receptor for either [3H]-methyltrienolone (Kd = 0.46 nM) or [3H]-dihydrotestosterone (Kd = 0.2 nM). Tritium 198-201 androgen receptor Rattus norvegicus 168-185 18308898-2 2008 Using site-directed mutagenesis, rhodopsin-based modeling, [(3)H](S)-(-)-N-(alpha-ethylbenzyl)-3-methoxy-2-phenylquinoline-4-carboxamide (Me-talnetant) and [(3)H]osanetant binding, and functional Schild analyses, we have demonstrated the important molecular determinants of neurokinin B (NKB), Me-talnetant, and osanetant binding pockets. Tritium 59-65 tachykinin precursor 3 Homo sapiens 274-286 6606427-4 1983 The glucocorticoid triamcinolone acetonide specifically inhibits both these androgen mediated events without altering the equilibrium dissociation constant (Kd) of the androgen receptor for either [3H]-methyltrienolone (Kd = 0.46 nM) or [3H]-dihydrotestosterone (Kd = 0.2 nM). Tritium 198-200 androgen receptor Rattus norvegicus 168-185 6605349-1 1983 A method is described for the analysis of [3H]-thymidine incorporation in microtitre cultures of bone marrow-derived mouse macrophage responding to macrophage colony-stimulating factor (CSF-1). Tritium 43-45 colony stimulating factor 1 (macrophage) Mus musculus 186-191 6605349-2 1983 [3H]-thymidine incorporation depends on cell density, culture medium, and the concentration of CSF-1 and serum, but is independent of Ca2+. Tritium 1-3 colony stimulating factor 1 (macrophage) Mus musculus 95-100 6139770-4 1983 The monoclonal antibody to choline acetyltransferase was radiolabeled in culture by incubating hybridomas in medium containing 3H-labeled amino acids. Tritium 127-129 choline O-acetyltransferase Rattus norvegicus 27-52 6139770-7 1983 Double labeling of tyrosine hydroxylase, a catecholaminergic marker, and choline acetyltransferase was carried out by reacting sections first with the 3H-labeled antibody to choline acetyltransferase and then with rabbit antibody to tyrosine hydroxylase. Tritium 151-153 choline O-acetyltransferase Rattus norvegicus 73-98 6226656-7 1983 The oligosaccharides of alpha 1-proteinase inhibitor secreted from 1-deoxynojirimycin-treated cells were characterized by their susceptibility to endoglucosaminidase H, incorporation of [3H]galactose, and [3H]fucose and concanavalin A-Sepharose chromatography. Tritium 187-189 serpin family A member 1 Rattus norvegicus 24-52 6226656-7 1983 The oligosaccharides of alpha 1-proteinase inhibitor secreted from 1-deoxynojirimycin-treated cells were characterized by their susceptibility to endoglucosaminidase H, incorporation of [3H]galactose, and [3H]fucose and concanavalin A-Sepharose chromatography. Tritium 206-208 serpin family A member 1 Rattus norvegicus 24-52 69440-7 1977 When the isolated proteins were labelled with 3H-labelled 4,4"-diisothiocyano-2,2"-ditritiostilbene disulfonic acid, the specific activity of the basic protein was 10 times that of lipophilin. Tritium 46-48 proteolipid protein 1 Homo sapiens 181-191 15470-0 1977 A tritium exchange method for characterization of modified histidyl residues and its application to fumarase. Tritium 2-9 fumarate hydratase Homo sapiens 100-108 838858-5 1977 After a single injection of casein the SAA level was elevated by 3h and peaked by 12-18 h. Similar levels were found in casein-treated A/J mice, a strain less susceptible to the induction of amyloid. Tritium 65-67 serum amyloid A cluster Mus musculus 39-42 824287-2 1976 [3H]Retinol was readily delivered from the RBP to the cells without a concomitant cellular uptake of the RBP. Tritium 1-3 retinol binding protein 4 Homo sapiens 43-46 824287-6 1976 The uptake of [3H]retinol by the cells could be inhibited by RBP containing unlabeled retinol, vitamin A-depleted RBP, and Fab" fragments against RBP. Tritium 15-17 retinol binding protein 4 Homo sapiens 61-64 824287-6 1976 The uptake of [3H]retinol by the cells could be inhibited by RBP containing unlabeled retinol, vitamin A-depleted RBP, and Fab" fragments against RBP. Tritium 15-17 retinol binding protein 4 Homo sapiens 114-117 824287-6 1976 The uptake of [3H]retinol by the cells could be inhibited by RBP containing unlabeled retinol, vitamin A-depleted RBP, and Fab" fragments against RBP. Tritium 15-17 retinol binding protein 4 Homo sapiens 114-117 6192909-4 1983 Further analysis of MM1 by antibody binding inhibition assay with 3H-labeled MM1-gamma 2b-1 antibody revealed that 8 of the 10 antibodies as well as monoclonal anti-Ly-6.2 showed significant inhibition. Tritium 66-68 immunoglobulin heavy constant gamma 2B Mus musculus 81-89 6619851-4 1983 At 130 min, [3H]deoxycytidine readily entered CSF, choroid plexus, and brain. Tritium 13-15 colony stimulating factor 2 Rattus norvegicus 46-49 18308898-2 2008 Using site-directed mutagenesis, rhodopsin-based modeling, [(3)H](S)-(-)-N-(alpha-ethylbenzyl)-3-methoxy-2-phenylquinoline-4-carboxamide (Me-talnetant) and [(3)H]osanetant binding, and functional Schild analyses, we have demonstrated the important molecular determinants of neurokinin B (NKB), Me-talnetant, and osanetant binding pockets. Tritium 59-65 tachykinin precursor 3 Homo sapiens 288-291 17988238-9 2008 In addition, expression of a constitutively active mutant of beta-catenin potently activated the canonical Wnt pathway and reduced [3H]thymidine incorporation. Tritium 132-134 catenin beta 1 Rattus norvegicus 61-73 6420526-3 1983 When [3H]-DNP-lys was preincubated with the immune serum obtained from rats immunized with dinitrophenylated bovine serum albumin (DNP-BSA), the [3H]-DNP-lys absorption was depressed. Tritium 6-8 albumin Rattus norvegicus 116-129 965373-20 1976 SCP1 was catalytically inactive when it was incubated with [3H] squalene and cofactors in the absence of microsomes. Tritium 60-62 synaptonemal complex protein 1 Rattus norvegicus 0-4 18441391-8 2008 The mu opioid ligand - [(3)H]-(D-Ala(2), -N-MePhe(4), Glyol(5)) Enkephalin was used to obtain binding characteristics of the control and TAA rat brain membranes. Tritium 23-29 proenkephalin Rattus norvegicus 64-74 181750-1 1976 3H-Labeled complementary DNA specific for the envelope glycoprotein (env) gene of avian leukosis-sarcoma viruses was isolated by selective nucleic acid hybridization techniques, and used to analyze the expression of the endogenous provirus. Tritium 0-2 endogenous retrovirus group K member 20 Homo sapiens 46-67 181750-1 1976 3H-Labeled complementary DNA specific for the envelope glycoprotein (env) gene of avian leukosis-sarcoma viruses was isolated by selective nucleic acid hybridization techniques, and used to analyze the expression of the endogenous provirus. Tritium 0-2 endogenous retrovirus group K member 20 Homo sapiens 46-49 6311560-7 1983 First, tyrosinase oxidation of [3H]-estradiol was markedly inhibited by sulfhydryl reducing agents (monothioglycerol) that stabilize [3H]-estradiol binding to estrogen receptor. Tritium 32-34 tyrosinase Homo sapiens 7-17 6311560-9 1983 We conclude that the [3H]-estradiol binding components in melanoma previously reported to be estrogen receptor probably represent instead the radiolabeled products of the tyrosinase-catalyzed oxidation of [3H]-estradiol. Tritium 22-24 tyrosinase Homo sapiens 171-181 6311560-9 1983 We conclude that the [3H]-estradiol binding components in melanoma previously reported to be estrogen receptor probably represent instead the radiolabeled products of the tyrosinase-catalyzed oxidation of [3H]-estradiol. Tritium 206-208 tyrosinase Homo sapiens 171-181 6308060-7 1983 Uptake of 10 nmol of [3H]bilirubin was 67.5 +/- 3.7% of the dose when injected with 125I-albumin, 67.4 +/- 6.5% when injected with 125I-ligandin, and 74.9 +/- 2.4% when injected with [14C]sucrose (P greater than 0.1). Tritium 22-24 glutathione S-transferase alpha 2 Rattus norvegicus 136-144 178500-1 1976 After the administration of 500 muC of 3H-5-HT the mast cells were degranulated by 1500 IU of PO B. Tritium 39-41 ER membrane protein complex subunit 3 Rattus norvegicus 94-98 17531458-2 2008 Our group has previously shown that both reduced folate carrier (RFC1) and folate receptor alpha (FRalpha) seem to be involved in the uptake of [3H]folic acid ([3H]FA) by a human trophoblast cell line (BeWo) and by human primary cultured cytotrophoblasts. Tritium 145-147 folate receptor alpha Homo sapiens 75-96 974794-0 1976 Distribution of 3H-alpha-MSH in rat brain. Tritium 16-18 proopiomelanocortin Rattus norvegicus 19-28 974794-5 1976 Large amounts of radioactivity after 3H-alpha-MSH were found in the pituitary and, particularly in the hypophysectomized rats, in the pineal. Tritium 37-39 proopiomelanocortin Rattus norvegicus 40-49 6355389-1 1983 Using [3H]corticosterone as a probe, corticosteroid-binding protein (CBP) was detected in eight out of eight isolates of Candida albicans, of both A and B serotypes. Tritium 7-9 CREB binding protein Mus musculus 69-72 6303356-2 1983 The involvement of 3H-methyl incorporation into protein carboxylmethyl esters during corticotropin releasing factor (CRF)-induced ACTH secretion from AtT-20/D16-16 mouse pituitary cells was investigated. Tritium 19-21 corticotropin releasing hormone Mus musculus 85-115 6219989-3 1983 Methylation and hydrolysis of the product formed in an incubation with UDP-GlcNAc and asialo-alpha 1-acid [3H]glycoprotein yielded 2,4,6-trimethyl [3H]galactose and 2,3,4-trimethyl [3H]galactose, indicating that N-acetylglucosaminyl residues were introduced to position C-3 and C-6 of the terminal galactoses on the glycoprotein. Tritium 107-109 complement C3 Homo sapiens 270-273 6188994-0 1983 Accumulation of [3H]fucose-labelled glycoproteins in the Golgi apparatus of dorsal root ganglion neurons during inhibition of fast axonal transport caused by exposure of the ganglion to Co2+-containing or Ca2+-free medium. Tritium 17-19 carbonic anhydrase 2 Homo sapiens 205-208 6188994-5 1983 In parallel studies where the radioactivity in nerve trunks and ganglia was measured by liquid scintillation counting, it was found that the Ca2+-free medium and the Co2+-containing medium both reduced by approximately 80% the quantity of [3H]fucose-labelled glycoproteins which were carried by the fast axonal transport system; they did so without interfering with the incorporation of [3H]fucose into glycoproteins. Tritium 240-242 carbonic anhydrase 2 Homo sapiens 141-144 238846-16 1975 The malate formed from 3S-3-hydroxy[2-3H2]butyryl-CoA on incubation with fumarase lost nearly 50%, that derived from 2R,3S-3-hydroxy[2-2H1,3H1]butyryl-CoA (plus 3R-diastereomer) retained about 26% and that derived from 2S,3S-3-hydroxy[2-1H1,3H1]butyryl-CoA retained about 78% of its total tritium content. Tritium 289-296 fumarate hydratase Homo sapiens 73-81 4383797-0 1967 Evidence for discrimination against tritium during oxidation of glucose via the glucose-6-phosphate dehydrogenase reaction. Tritium 36-43 glucose-6-phosphate dehydrogenase Homo sapiens 80-113 17531458-2 2008 Our group has previously shown that both reduced folate carrier (RFC1) and folate receptor alpha (FRalpha) seem to be involved in the uptake of [3H]folic acid ([3H]FA) by a human trophoblast cell line (BeWo) and by human primary cultured cytotrophoblasts. Tritium 145-147 FOS like 1, AP-1 transcription factor subunit Homo sapiens 98-105 17531458-2 2008 Our group has previously shown that both reduced folate carrier (RFC1) and folate receptor alpha (FRalpha) seem to be involved in the uptake of [3H]folic acid ([3H]FA) by a human trophoblast cell line (BeWo) and by human primary cultured cytotrophoblasts. Tritium 161-163 folate receptor alpha Homo sapiens 75-96 17531458-2 2008 Our group has previously shown that both reduced folate carrier (RFC1) and folate receptor alpha (FRalpha) seem to be involved in the uptake of [3H]folic acid ([3H]FA) by a human trophoblast cell line (BeWo) and by human primary cultured cytotrophoblasts. Tritium 161-163 FOS like 1, AP-1 transcription factor subunit Homo sapiens 98-105 6757247-5 1982 In confirmation of the trimming reaction observed in vivo, 3H-labeled Man9-N-acetylglucosaminitol from bovine thyroglobulin and [14C]Man9GlcNAc2 from yeast oligosaccharide-lipid were converted in vitro by broken yeast cells to 3H-labeled Man8-N-acetylglucosaminitol and [14C]Man8GlcNAc2. Tritium 59-61 mannosidase alpha class 1A member 1 Homo sapiens 70-74 17531458-6 2008 On the other hand, [3H]FA uptake was significantly increased by chronic exposure to xanthohumol, quercetin or isoxanthohumol (0.1-10 microM), and this increase does not seem to result from changes in the level of RFC1 or FRalpha gene expression. Tritium 20-22 FOS like 1, AP-1 transcription factor subunit Homo sapiens 221-228 6757252-7 1982 This series of reactions requires the actions of T4-coded ribonucleoside diphosphate reductase and its associated reducing system, dCTP/dUTPase and dCMP hydroxymethylase, 3H being lost to water at the last step. Tritium 171-173 Deoxyuridine triphosphatase Drosophila melanogaster 131-143 34047146-5 2021 When enzyme inhibition activities were evaluated, it was determined that the compounds 3a (42.33%) and 3d (42.39%) on acetylcholinesterase (AChE) enzyme; compounds 3g (75.42%) and 3h (60.33%) showed inhibition on MAO-B enzyme at most, at 10-3 M concentration. Tritium 180-182 monoamine oxidase B Homo sapiens 213-218 17692995-6 2008 LPA also inhibited [3H]-glutamate uptake in both undifferentiated and differentiated astrocytes; and LPA-induced inhibition of glutamate uptake was still observed in lpa1-deficient astrocytes. Tritium 20-22 lysophosphatidic acid receptor 1 Mus musculus 166-170 33146444-7 2021 LEED and STM assisted by theoretical simulations have been employed to characterize in detail these novel 2D conglomerates with relevant chiral properties for systems with C 3h symmetry. Tritium 174-176 sulfotransferase family 1A member 3 Homo sapiens 9-12 7161265-10 1982 The inhibition of binding between 3H-labeled rat sulfite oxidase and its antibody by unlabeled enzymes from other animals demonstrated that 10 to 20% of the antibody could cross-react with these enzymes. Tritium 34-36 sulfite oxidase Rattus norvegicus 49-64 6981669-7 1982 Regarding the specificity of binding, (3H) CS-C binding to lymphocytes was inhibited with CS-A and CS-C; however, no inhibition with the biologically inert keto-CS-A was observed. Tritium 38-41 chorionic somatomammotropin hormone 1 Homo sapiens 90-94 33374302-7 2020 Gene knockdown of LAT1 (l-type amino acid transporter 1) and CAT1 (cationic amino acid transporter 1) demonstrated that LAT1 is primarily involved in the transport of [3H]l-tryptophan in both TR-BBB and NSC-34 cell lines. Tritium 168-170 solute carrier family 7 (cationic amino acid transporter, y+ system), member 5 Mus musculus 24-55 18027973-6 2007 The most potent, nonselective member of this class of Nav1.7 blockers has been radiolabeled with tritium. Tritium 97-104 sodium voltage-gated channel alpha subunit 9 Rattus norvegicus 54-60 33374302-7 2020 Gene knockdown of LAT1 (l-type amino acid transporter 1) and CAT1 (cationic amino acid transporter 1) demonstrated that LAT1 is primarily involved in the transport of [3H]l-tryptophan in both TR-BBB and NSC-34 cell lines. Tritium 168-170 solute carrier family 7 (cationic amino acid transporter, y+ system), member 5 Mus musculus 120-124 32711231-4 2020 The most potent SIRT1 inhibitor 3h, bearing an isopropyl substituent, was as potent as EX-527, and more selective for SIRT1 over SIRT2. Tritium 32-34 sirtuin 1 Homo sapiens 16-21 32711231-4 2020 The most potent SIRT1 inhibitor 3h, bearing an isopropyl substituent, was as potent as EX-527, and more selective for SIRT1 over SIRT2. Tritium 32-34 sirtuin 1 Homo sapiens 118-123 7096539-4 1982 The free glucocorticoid receptor-binding activity was determined by the extent to which steroids present in the sample inhibit binding of the 3H-labeled steroids. Tritium 142-144 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 9-32 18027973-7 2007 [3H]BNZA binds with high affinity to rat brain synaptosomal membranes (Kd = 1.5 nM) and to membranes prepared from HEK293 cells stably transfected with hNav1.5 (Kd = 0.97 nM). Tritium 1-3 sodium voltage-gated channel alpha subunit 5 Homo sapiens 152-159 6955571-3 1982 17 beta-SDH activity was measured by incubating intact cell monolayers with [3H]-estradiol (5 x 10(-9) M) in serum-free medium. Tritium 77-79 succinate dehydrogenase complex iron sulfur subunit B Homo sapiens 8-11 17920288-3 2007 Among tested flavonoids, morin and silybin exhibited significant inhibition effects on the cellular uptake of [3H]-para-aminohippuric acid ([3H]-PAH) in MDCK-hOAT1 cells with Ki of 0.46 microM and 24 microM, respectively, while all the tested flavonoids appeared to be less interactive with hOAT3 compared to hOAT1. Tritium 111-113 solute carrier family 22 member 6 Homo sapiens 158-163 6179567-6 1982 Addition of Ca2+ (1.1 mM) during the 12-h period stimulated AXT, measured as accumulation of 3H-labelled components in front of a ligature, several fold. Tritium 93-95 contactin 2 Homo sapiens 60-63 7044130-4 1982 These 3H-labeled LLSLs were also found to be predominantly Thy-1+, Lyt-1+2-. Tritium 6-8 CD5 antigen Mus musculus 67-72 32785591-4 2020 [3H]-labeled cholesterol was used to assess the levels of cholesterol efflux, and the results showed that IL-5 increased ABCA1-mediated cholesterol efflux. Tritium 1-3 ATP binding cassette subfamily A member 1 Homo sapiens 121-126 32561573-5 2020 3-D brain images of KtdT and immunohistochemistry (IHC) on brain sections with antibodies against tdTomato show similar distribution to that of autoradiography of [3H]U69,593 binding to KOR in wildtype mice. Tritium 164-166 opioid receptor, kappa 1 Mus musculus 186-189 31842924-8 2019 RESULTS: In vitro BBB and in silico transporter studies indicated that [3H]amisulpride and [3H]haloperidol were transported by the influx transporter, OCT1, and efflux transporters MATE1 and PMAT. Tritium 72-74 solute carrier family 22 (organic cation transporter), member 1 Mus musculus 151-155 31842924-8 2019 RESULTS: In vitro BBB and in silico transporter studies indicated that [3H]amisulpride and [3H]haloperidol were transported by the influx transporter, OCT1, and efflux transporters MATE1 and PMAT. Tritium 72-74 solute carrier family 47 member 1 Homo sapiens 181-186 31375704-4 2019 When present during co-polymerization of Tpm3.1 with actin, 3H-ATM-3507 is incorporated into the filaments and saturates at approximately one molecule per Tpm3.1 dimer and with an apparent binding affinity of approximately 2 microM. Tritium 60-62 tropomyosin 3 Homo sapiens 41-45 31375704-4 2019 When present during co-polymerization of Tpm3.1 with actin, 3H-ATM-3507 is incorporated into the filaments and saturates at approximately one molecule per Tpm3.1 dimer and with an apparent binding affinity of approximately 2 microM. Tritium 60-62 tropomyosin 3 Homo sapiens 155-159 7041983-6 1982 For example, in preparations in which the esterified cholesterol content of the labelled HDL was increased by the action of lecithin: cholesterol acyltransferase, there was a reduced rate of transfer of esterified [3H]cholesterol out of the HDL fraction in subsequent incubations of the labelled HDL with fresh, unlabelled plasma. Tritium 215-217 lecithin-cholesterol acyltransferase Homo sapiens 124-161 17920288-4 2007 A kinetic study suggested that morin and silybin inhibited hOAT1-mediated cellular uptake of [3H]-PAH in a competitive manner. Tritium 94-96 solute carrier family 22 member 6 Homo sapiens 59-64 7061225-1 1982 The effect of human prealbumin fraction as allogeneic cell-mediated immunity in primary sensitization cultures of murine spleen cells was studied by 3H-thymidine uptake and specific 51Cr release assays. Tritium 149-151 transthyretin Mus musculus 20-30 30587573-6 2019 Assembly with Kir6.2 reduced SUR1"s KD for [3H]GBM. Tritium 44-46 potassium inwardly rectifying channel subfamily J member 11 Homo sapiens 14-20 30587573-8 2019 Moreover, ATP/GBM site coupling was constrained in WT SUR1/WT Kir6.2 channels; ATP-bound channels had a lower KD for [3H]GBM than ATP-bound SUR1. Tritium 118-120 potassium inwardly rectifying channel subfamily J member 11 Homo sapiens 62-68 17600318-8 2007 In functional studies, the HO-1 inhibitor, ZnPP-IX, partly reversed the growth-inhibitory effects of BMP4, and overexpression of HO-1 in PASMCs inhibited serum-stimulated [3H]-thymidine incorporation. Tritium 172-174 heme oxygenase 1 Homo sapiens 27-31 7061595-4 1982 In situ hybridization of 3H-labeled cloned DHFR cDNA to metaphase chromosomes of L5178YR cells was used to localize the DHFR genes to a very large Giemsa (G)-negative region on each of the two large marker chromosomes. Tritium 25-27 dihydrofolate reductase Mus musculus 120-124 17600318-8 2007 In functional studies, the HO-1 inhibitor, ZnPP-IX, partly reversed the growth-inhibitory effects of BMP4, and overexpression of HO-1 in PASMCs inhibited serum-stimulated [3H]-thymidine incorporation. Tritium 172-174 heme oxygenase 1 Homo sapiens 129-133 7061595-4 1982 In situ hybridization of 3H-labeled cloned DHFR cDNA to metaphase chromosomes of L5178YR cells was used to localize the DHFR genes to a very large Giemsa (G)-negative region on each of the two large marker chromosomes. Tritium 25-27 dihydrofolate reductase Mus musculus 43-47 17631923-1 2007 [3H]Epibatidine binds to nAChR subtypes in mouse brain with higher (KD approximately 0.02 nM) and lower affinity (KD approximately 7 nM), which can be further subdivided through inhibition by selected agonists and antagonists. Tritium 1-3 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 25-30 30481645-6 2019 Also, compounds 3a (IC50 = 0.114 microM), 3h (IC50 = 0.049 microM), and 3i (IC50 = 0.054 microM) were the most active derivatives against MAO-B enzyme activity. Tritium 42-44 monoamine oxidase B Homo sapiens 138-143 17631923-4 2007 Deletion of the alpha7, beta2 or beta4 nicotinic receptor subunit genes identifies highly expressed subtypes with relatively low affinity for [3H]epibatidine. Tritium 143-145 cholinergic receptor, nicotinic, alpha polypeptide 7 Mus musculus 16-22 6958493-7 1982 The glucocorticoid receptor was found to exist in two forms with Stokes radii of 6.1 and 3.6 nm, respectively, following in vivo labelling with [3H] dexamethasone. Tritium 145-147 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 4-27 17631923-4 2007 Deletion of the alpha7, beta2 or beta4 nicotinic receptor subunit genes identifies highly expressed subtypes with relatively low affinity for [3H]epibatidine. Tritium 143-145 basic helix-loop-helix family, member e23 Mus musculus 33-38 17512915-0 2007 [3H]-L-685,458 as a radiotracer that maps gamma-secretase complex in the rat brain: relevance to Abeta genesis and presence of active presenilin-1 components. Tritium 1-3 presenilin 1 Rattus norvegicus 134-146 6275419-2 1981 The [3H] leu-enkephalin [LE] binding to brain homogenates from swum mice was significantly reduced when compared with that form unswum controls. Tritium 5-7 prodynorphin Mus musculus 9-23 7197683-8 1981 When isolated nuclei are incubated with [3H]retinol-CRBP, sectioned, and autoradiographed, specifically bound retinol is found distributed throughout the nuclei. Tritium 41-43 retinol binding protein 1 Rattus norvegicus 52-56 30617930-6 2019 Our results show that NLRP7 (i) is predominantly expressed in the trophoblasts during the hypoxic period of placental development and its expression is upregulated by hypoxia and (ii) increases trophoblast proliferation ([3H]-thymidine) and controls the precocious differentiation of trophoblasts towards syncytium (syncytin 1 and 2 and beta-hCG production and xCELLigence analysis) and towards invasive extravillous trophoblast (2D and 3D cultures). Tritium 222-224 NLR family pyrin domain containing 7 Homo sapiens 22-27 17397826-9 2007 Ibu-am5 inhibited the binding of [3H]-CP55,940 to rat brain CB1 and human CB2 cannabinoid receptors more potently than ibuprofen, but the increase in potency was less than the corresponding increase in potency seen for inhibition of FAAH activity. Tritium 34-36 cannabinoid receptor 2 Homo sapiens 74-77 30668375-10 2019 RESULTS: Eta inhibited the TRPA1 agonist-induced Ca2+ influx [Imax = 72.4 +- 1.5%; IC50 = 0.023(0.004-0.125)microg/ml], but not TRPV1 agonist-induced, nor was able to displace [3H]-resiniferatoxin (TRPV1 agonist) binding. Tritium 177-179 transient receptor potential cation channel, subfamily A, member 1 Mus musculus 27-32 30326345-1 2019 BACKGROUND: We previously reported that, compared to controls, there are lower levels of [3H]LY341495 binding to metabotropic 2/3 receptors (GRM2/3) in Brodmann"s area (BA) 24, but not 17 or 46, from subjects with major depressive disorders (MDD) but not bipolar disorders (BD) or schizophrenia. Tritium 90-92 glutamate metabotropic receptor 2 Homo sapiens 141-147 30326345-8 2019 Given we have found lower levels of [3H]LY341495 binding to GRM2/3 in BA 24 from subjects with MDD, our new data argues the lower levels of radioligand binding was due to lower levels of GRM3. Tritium 37-39 glutamate metabotropic receptor 2 Homo sapiens 60-66 6119632-5 1981 [3H]Glutamate binding to P1 and P2 fractions was effectively displaced by several structural analogues of glutamate. Tritium 1-3 perforin 1 Gallus gallus 25-34 6265476-1 1981 A rat cerebellar cell line, WC5, derived by transformation with Rous sarcoma virus, which is temperature-sensitive for transformation (ts-RSV), can be induced to express glial fibrillary acidic protein (GFAP). Tritium 135-137 glial fibrillary acidic protein Rattus norvegicus 170-201 6265476-1 1981 A rat cerebellar cell line, WC5, derived by transformation with Rous sarcoma virus, which is temperature-sensitive for transformation (ts-RSV), can be induced to express glial fibrillary acidic protein (GFAP). Tritium 135-137 glial fibrillary acidic protein Rattus norvegicus 203-207 7256194-6 1981 Experiments with HDL labelled with [3H] cholesterol by means of lecithin: cholesterol acyltransferase (LCAT, EC 2.3.1.43) in the cholesterol ester moiety suggested that the same mechanism was responsible for the cell-association of HDL prepared this way. Tritium 36-38 lecithin cholesterol acyltransferase Rattus norvegicus 64-101 7256194-6 1981 Experiments with HDL labelled with [3H] cholesterol by means of lecithin: cholesterol acyltransferase (LCAT, EC 2.3.1.43) in the cholesterol ester moiety suggested that the same mechanism was responsible for the cell-association of HDL prepared this way. Tritium 36-38 lecithin cholesterol acyltransferase Rattus norvegicus 103-107 6166353-2 1981 X-537 A at 0.09 muM blocked AXT of [3H]labelled proteins by 50% as judged by ligature experiments while the levels of ATP and CrP remained unchanged. Tritium 36-38 contactin 2 Homo sapiens 28-31 30620754-2 2019 Activation of PPARdelta by GW501516, a specific ligand of PPARdelta, significantly inhibited Ang II-stimulated protein synthesis in a concentration-dependent manner, as determined by [3H]-leucine incorporation. Tritium 184-186 peroxisome proliferator activated receptor delta Homo sapiens 14-23 30620754-2 2019 Activation of PPARdelta by GW501516, a specific ligand of PPARdelta, significantly inhibited Ang II-stimulated protein synthesis in a concentration-dependent manner, as determined by [3H]-leucine incorporation. Tritium 184-186 peroxisome proliferator activated receptor delta Homo sapiens 58-67 30620754-4 2019 Transfection of small interfering RNA (siRNA) against PPARdelta significantly reversed the effects of GW501516 on [3H]-leucine incorporation and ROS generation, indicating that PPARdelta is involved in these effects. Tritium 115-117 peroxisome proliferator activated receptor delta Homo sapiens 54-63 17229541-6 2007 Moreover, disturbed dendritic structure in the CA1 and CA3 stratum radiatum was manifested as formation of varicosities 3h after the kainic acid treatment. Tritium 120-122 carbonic anhydrase 1 Rattus norvegicus 47-50 30620754-4 2019 Transfection of small interfering RNA (siRNA) against PPARdelta significantly reversed the effects of GW501516 on [3H]-leucine incorporation and ROS generation, indicating that PPARdelta is involved in these effects. Tritium 115-117 peroxisome proliferator activated receptor delta Homo sapiens 177-186 30108159-2 2018 Both [3H]BUP and [3H]SAM bound to the mu-, kappa-, and delta-opioid receptors (MOR, KOR, and DOR, respectively) with Kd values of 3 nM or less. Tritium 6-8 opioid receptor kappa 1 Homo sapiens 84-87 30108159-2 2018 Both [3H]BUP and [3H]SAM bound to the mu-, kappa-, and delta-opioid receptors (MOR, KOR, and DOR, respectively) with Kd values of 3 nM or less. Tritium 18-20 opioid receptor kappa 1 Homo sapiens 84-87 6171467-1 1981 This investigation was to study the biosynthesis of 3H-labeled alpha-fetoprotein (AFP) by cultured mouse hepatoma (HEPA-2) cells. Tritium 52-54 alpha fetoprotein Mus musculus 63-80 6171467-1 1981 This investigation was to study the biosynthesis of 3H-labeled alpha-fetoprotein (AFP) by cultured mouse hepatoma (HEPA-2) cells. Tritium 52-54 alpha fetoprotein Mus musculus 82-85 7011615-3 1980 Using immunofluorescence and pulse-label studies with 3H-labelled amino acids, histamine was shown to inhibit the secretion of newly synthesized C2, C4, C3, factor B and beta 1H globulin by monocytes in culture. Tritium 54-56 complement factor H Homo sapiens 170-177 29599133-5 2018 LAL-deficient mice injected intraperitoneally with lal-/- macrophages cholesterol loaded and labeled in the same way exhibited only 1.55+-0.35% total injected [3H]-cholesterol counts appearing in the feces for 48 h (n=30), compared with 5.38+-0.92% in lal+/+ mice injected with labeled lal+/+ macrophages (n=27), P<0.001. Tritium 160-162 lysosomal acid lipase A Mus musculus 51-54 29599133-6 2018 To mimic the therapeutic condition of delivery of supplemental LAL in vivo, injection of labeled lal-/- macrophages into lal+/+ mice resulted in a significant increase in reverse cholesterol transport (2.60+-0.46% of 3H-cholesterol counts in feces at 48 hours [n=19]; P<0.001 when compared with injection into lal-/- mice). Tritium 217-219 lysosomal acid lipase A Mus musculus 97-100 17234339-3 2007 Treatment of mouse dopaminergic CATH.a cells with 2 mM methamphetamine increased PAG608 expression at 3h followed by increase in phosphorylated p53 expression. Tritium 102-104 zinc finger, matrin type 3 Rattus norvegicus 81-87 7408860-1 1980 Fumarase does not catalyse 18O exchange between (2S,3R)-tartrate and solvent water, nor does it catalyse 2H or 3H exchange into (2S)-fluorosuccinate from isotopically labelled water. Tritium 111-113 fumarate hydratase Homo sapiens 0-8 17143559-3 2007 The immuno-blockade of GAL-R1 and GAL-R2, but not GAL-R3, decreased the binding of [3H]galanin to dispersed cells, a complete inhibition being obtained only by the simultaneous blockade of both receptor subtypes. Tritium 84-86 galanin receptor 1 Rattus norvegicus 23-40 7429074-2 1980 This transfer constitutes a major contribution to the rate of incorporation of 3H-labeled transcripts into polysomal MHC mRNA at this time. Tritium 79-81 myosin, heavy chain 11, smooth muscle Gallus gallus 117-120 7429074-3 1980 As the increase in the rate of MHC synthesis levels off (late-to post-fusion) the contribution to the rate of incorporation of 3H-labeled transcripts into polysomal MHC mRNA from newly synthesized transcripts increases until it becomes predominant. Tritium 127-129 myosin, heavy chain 11, smooth muscle Gallus gallus 31-34 7429074-3 1980 As the increase in the rate of MHC synthesis levels off (late-to post-fusion) the contribution to the rate of incorporation of 3H-labeled transcripts into polysomal MHC mRNA from newly synthesized transcripts increases until it becomes predominant. Tritium 127-129 myosin, heavy chain 11, smooth muscle Gallus gallus 165-168 29259130-4 2018 siRNA-mediated knockdown of cathepsin B in McA-RH7777 cells resulted in a 39% increase in [3H]TAG associated with VLDL secretion. Tritium 91-93 cathepsin B Mus musculus 28-39 29414147-5 2018 RESULTS: In SH-SY5Y cells, [3H]-dopamine uptake was inhibited by desipramine (selective NET inhibitor), GBR-12909 (selective DAT inhibitor), and fluoxetine (selective inhibitor of the serotonin transporter, SERT) with IC50 values 37, 537, and 2800nM, respectively. Tritium 28-30 solute carrier family 6 member 3 Homo sapiens 125-128 29304533-5 2018 Transport studies in membrane vesicles of Sf9 cells containing recombinant human MRP4 revealed that MRP4 mediates ATP-dependent transport of fluorescein- and tritium-labelled S1P. Tritium 158-165 ATP binding cassette subfamily C member 4 Homo sapiens 81-85 29304533-5 2018 Transport studies in membrane vesicles of Sf9 cells containing recombinant human MRP4 revealed that MRP4 mediates ATP-dependent transport of fluorescein- and tritium-labelled S1P. Tritium 158-165 ATP binding cassette subfamily C member 4 Homo sapiens 100-104 28189393-7 2017 In addition, compound 3h (DY181) was identified as the most potent synthetic inverse agonist for the ERRbeta that exhibited excellent selectivity over ERRalpha/gamma in functional assays. Tritium 22-24 estrogen related receptor beta Homo sapiens 101-108 6246936-2 1980 8-N3cAMP is able to support the binding of (3H)d(I-C)n by CRP, indicating that it is a functional cAMP analogue. Tritium 44-46 catabolite gene activator protein Escherichia coli 58-61 6781743-3 1980 Analyses by Sephadex LH-20 column chromatography of hydrocarbon-deoxyribonucleoside adducts obtained after enzymic digestion of the [3H]DMBA-modified DNA revealed that the products of binding of DMBA to DNA in the presence of the S-9 fraction vary with the relative concentration of DMBA to S-9 fraction. Tritium 133-135 proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 Mus musculus 230-233 6781743-3 1980 Analyses by Sephadex LH-20 column chromatography of hydrocarbon-deoxyribonucleoside adducts obtained after enzymic digestion of the [3H]DMBA-modified DNA revealed that the products of binding of DMBA to DNA in the presence of the S-9 fraction vary with the relative concentration of DMBA to S-9 fraction. Tritium 133-135 proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 Mus musculus 291-294 500787-3 1979 Molecular hybridization of a purified [3H]DNA probe complimentary to DHFR specific mRNA with cellular DNA and RNA indicates that DHFR coding sequences are elevated several hundred fold in both nucleic acid species in the mutant cell line. Tritium 39-41 dihydrofolate reductase Mus musculus 129-133 17074421-4 2007 Trx mRNA and protein levels in neurons with mitochondrial dysfunction decreased in a dose- and time-dependent manner (mRNA: 25-150 microM H(2)O(2) for 1h and 50 microM H(2)O(2) for 1-3h; protein: 25-150 microM H(2)O(2) for 1h and 50 microM H(2)O(2) for 1-4h), while those in control neurons had no significant changes (50-250 microM H(2)O(2) for 1h). Tritium 183-185 thioredoxin Homo sapiens 0-3 479195-1 1979 The [3H]-triamcinolone acetonide-labeled glucocorticoid receptor from rat liver cytosol was purified to 85% homogeneity according to sodium dodecyl sulfate gel electrophoresis. Tritium 5-7 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 41-64 573265-1 1979 Specific antibodies against cellular retinol-binding protein (CRBP), raised in rabbit, were detected by sucrose gradient centrifugation and gel filtration using tritium-labeled CRBP, prepared by reductive methylation. Tritium 161-168 retinol binding protein 1 Rattus norvegicus 62-66 573265-1 1979 Specific antibodies against cellular retinol-binding protein (CRBP), raised in rabbit, were detected by sucrose gradient centrifugation and gel filtration using tritium-labeled CRBP, prepared by reductive methylation. Tritium 161-168 retinol binding protein 1 Rattus norvegicus 177-181 27586561-0 2017 Changes in [3H]-ouabain and [3H]-neurotensin binding to rat cerebral cortex membranes after administration of antipsychotic drugs haloperidol and clozapine. Tritium 29-31 neurotensin Rattus norvegicus 33-44 27586561-3 2017 Assays of high affinity [3H]-ouabain binding (to analyze K+ site of Na+, K+-ATPase) show that in vitro addition of neurotensin decreases binding. Tritium 25-27 neurotensin Rattus norvegicus 115-126 27586561-5 2017 To test the involvement of dopaminergic D2 receptors in [3H]-ouabain binding inhibition by neurotensin, Wistar rats were administered i.p.with antipsychotic drugs haloperidol (2mg/kg) and clozapine (3, 10 and 30mg/kg). Tritium 57-59 neurotensin Rattus norvegicus 91-102 27586561-11 2017 To analyze the interaction between dopaminergic D2 and NTS1 receptors, [3H]-neurotensin binding to cortical membranes from rats injected with haloperidol (2mg/kg, i.p.) Tritium 72-74 neurotensin Rattus norvegicus 76-87 17156375-4 2006 We co-transfected Ltk- cells with increasing amounts of alpha-Syn DNA and a constant amount of NET DNA, and observed a progressive decrease (68%) in [3H]-NE uptake in cells co-transfected with a ratio of 3:1 alpha-Syn:NET DNA. Tritium 150-152 leukocyte receptor tyrosine kinase Homo sapiens 18-21 27939322-8 2017 Using [3H]PK 11195 as a radio ligand, we found that TSPO binding sites are not uniform, but separated into two sub-populations, one with high affinity (respective Kd and Bmax values of 1.40+-0.08nM and 1586+-48 fmol/mg protein), another with lower affinity (respective Kd and Bmax values of 61+-5nM and 26260+-1050 fmol/mg protein). Tritium 7-9 translocator protein Homo sapiens 52-56 27656777-11 2016 Finally, intravenous injection of tritium-radiolabeled 3H-VH4127 in wild-type or ldlr -/- mice confirmed their active LDLR targeting in vivo. Tritium 34-41 low density lipoprotein receptor Mus musculus 81-85 231087-4 1979 Virions containing 3H-labelled DNA were used to infect ts BTN-1 cells at 39.5 degrees C, and the total cellular DNA isolated from these cells was resolved into host and virus material by repeated CsCl equilibrium gradient centrifugation. Tritium 19-21 butyrophilin subfamily 1 member A1 Homo sapiens 58-63 17156375-4 2006 We co-transfected Ltk- cells with increasing amounts of alpha-Syn DNA and a constant amount of NET DNA, and observed a progressive decrease (68%) in [3H]-NE uptake in cells co-transfected with a ratio of 3:1 alpha-Syn:NET DNA. Tritium 150-152 synuclein alpha Homo sapiens 56-65 27656777-11 2016 Finally, intravenous injection of tritium-radiolabeled 3H-VH4127 in wild-type or ldlr -/- mice confirmed their active LDLR targeting in vivo. Tritium 34-41 low density lipoprotein receptor Mus musculus 118-122 27656777-11 2016 Finally, intravenous injection of tritium-radiolabeled 3H-VH4127 in wild-type or ldlr -/- mice confirmed their active LDLR targeting in vivo. Tritium 55-57 low density lipoprotein receptor Mus musculus 81-85 435461-4 1979 The configurational purity of tritium at C-2 of dopamine and C-1 of the dopamine precursor 3-methoxy-4-hydroxyphenethylamine has been confirmed employing dopamine-beta-hydroxylase (specific for the pro-R hydrogen at C-2) and pea seedling amine oxidase (specific for the pro-S hydrogen at C-1). Tritium 30-37 complement C2 Bos taurus 41-44 16889765-4 2006 3H-thymidine incorporation by fresh rat and mouse hepatocytes was maximal in cultures treated with 10 and 5 ng/ml EGF, respectively. Tritium 0-2 epidermal growth factor Mus musculus 114-117 435461-4 1979 The configurational purity of tritium at C-2 of dopamine and C-1 of the dopamine precursor 3-methoxy-4-hydroxyphenethylamine has been confirmed employing dopamine-beta-hydroxylase (specific for the pro-R hydrogen at C-2) and pea seedling amine oxidase (specific for the pro-S hydrogen at C-1). Tritium 30-37 complement C2 Bos taurus 216-219 86511-3 1979 Immunoprecipitation of protein-bound [3H]-oestradiol from solution was proportional to anti-AFP immunoglobulin G concentration. Tritium 38-40 alpha fetoprotein Mus musculus 92-95 27656777-11 2016 Finally, intravenous injection of tritium-radiolabeled 3H-VH4127 in wild-type or ldlr -/- mice confirmed their active LDLR targeting in vivo. Tritium 55-57 low density lipoprotein receptor Mus musculus 118-122 26790758-5 2016 State anxiety as well as circulating interleukin (IL)-6, tumor necrosis factor (TNF)-alpha and IL-10 concentrations were significantly increased 2h and 3h after LPS administration, with a peak at 2h, and returned to baseline 6h after administration. Tritium 152-154 interleukin 10 Homo sapiens 95-100 26784349-6 2016 RESULTS: SAA increased IL-6 (31-fold) and TNFalpha (6.5-fold) mRNA levels compared to control untreated cells after 3h of SAA treatment, and increased secreted IL-6 protein at 24h. Tritium 116-118 serum amyloid A1 cluster Homo sapiens 9-12 427875-2 1979 LIF stopped in vitro cell multiplication by blocking the G1--S transition, and reduced in vivo [3H]thymidine incorporation into liver DNA in two-thirds hepatectomized rats. Tritium 96-98 LIF, interleukin 6 family cytokine Rattus norvegicus 0-3 225357-1 1979 Binding sites for [3H]cAMP on purified regulatory dimers of type II A-kinase (II-R2) are independent as assessed by equilibrium binding (KD = 6 +/- 1.3 nM at pH 7.2, 25 degrees; nH = 1.0) and by the lack of effect of unlabeled cAMP on dissociation rate (kd = 1.0 X 10(-3) sec -1 at pH 7.2, 25 degrees). Tritium 19-21 secretory blood group 1, pseudogene Homo sapiens 272-278 225357-2 1979 In contrast, binding sites for [3H]cGMP on purified G-kinase displayed positively cooperative interactions in both equilibrium and dissociation assays with convex upward Scatchard plots, an nH of 1.6 and a dissociation rate (kd = 6.2 X 10(-3) sec-1 at pH 6.8, 0 degree) which was slowed by excess unlabeled cGMP (kd = 1.13 X 10(-3) sec-1 at pH 6.8, degree). Tritium 32-34 secretory blood group 1, pseudogene Homo sapiens 243-248 225357-2 1979 In contrast, binding sites for [3H]cGMP on purified G-kinase displayed positively cooperative interactions in both equilibrium and dissociation assays with convex upward Scatchard plots, an nH of 1.6 and a dissociation rate (kd = 6.2 X 10(-3) sec-1 at pH 6.8, 0 degree) which was slowed by excess unlabeled cGMP (kd = 1.13 X 10(-3) sec-1 at pH 6.8, degree). Tritium 32-34 secretory blood group 1, pseudogene Homo sapiens 332-337 27725436-6 2016 Consistent with these results, TPAs and TPBi blocked the binding of [3H]9cRA to RXR and of [3H]Rosi to PPARgamma in a competitive manner. Tritium 69-71 retinoid X receptor alpha Homo sapiens 80-83 16839579-0 2006 Nuclear type II [3H]estradiol binding site ligands: inhibition of ER-positive and ER-negative cell proliferation and c-Myc and cyclin D1 gene expression. Tritium 17-19 MYC proto-oncogene, bHLH transcription factor Homo sapiens 117-122 711133-2 1978 The 3H-leucine incorporation in thyroid proteins (dpm/mg) was determined following incubation of slices for 1 h and 2 h. Insulin given in situ significantly increased the mean incorporation into homogenate and cytosol proteins: about 130% after 1 h of incubation, for an identical hypoglycaemia in both lobes. Tritium 4-6 insulin Canis lupus familiaris 121-128 16715497-1 2006 Preincubation of striatal slices with the selective histamine H3-receptor agonist immepip (100 nM) decreased the specific binding of N-alpha-[methyl-3H]-histamine ([3H]-NMHA) to membranes obtained from the treated slices. Tritium 149-151 histamine receptor H3 Rattus norvegicus 52-73 705782-0 1978 Excretion and tissue distribution of radioactivity from tritium-labeled T-2 toxin in chicks. Tritium 56-63 solute carrier family 25 member 5 Homo sapiens 72-75 26400551-4 2015 Its binding to GPER was achieved by competition assays with [3H]E2 and [5,6-3H] nicotinic acid in ER-negative and GPER-positive SkBr3 breast cancer cells. Tritium 61-63 G protein-coupled estrogen receptor 1 Homo sapiens 15-19 16122793-4 2006 PAF was measured by PAF [3H] scintillation proximity assay (SPA) system. Tritium 25-27 PCNA clamp associated factor Homo sapiens 0-3 26032487-3 2015 Among these compounds, compound 3h exhibited the most potent inhibitory activities against VEGFR2 tyrosine kinase and cell proliferation, with the IC50 values of 2.89 nm (for VEGFR2) and 0.25 mum (for MCF-7), which were comparable with the control compound. Tritium 32-34 kinase insert domain receptor Homo sapiens 91-97 26305376-4 2015 Co-expression of DAT and DJ-1 in HEK-293T cells leads to an increase in [3H] dopamine uptake that does not appear to be mediated by increased total DAT expression but rather through an increase in DAT cell surface localization. Tritium 73-75 solute carrier family 6 member 3 Homo sapiens 17-20 26305376-7 2015 Co-expression of a mini-gene that can disrupt the DAT/DJ-1 complex appears to block the increase in [3H] dopamine uptake by DJ-1. Tritium 101-103 solute carrier family 6 member 3 Homo sapiens 50-53 661626-1 1978 Incorporation of 3H-TdR into EL4 leukemic cells in vitro was inhibited by peritoneal exudate cells (PEC) harvested from syngeneic C57BL/6J mice given an intraperitoneal (i.p.) Tritium 17-19 epilepsy 4 Mus musculus 29-32 16122793-4 2006 PAF was measured by PAF [3H] scintillation proximity assay (SPA) system. Tritium 25-27 PCNA clamp associated factor Homo sapiens 20-23 734357-1 1978 3H-serotonin release from guinea-pig platelets was demonstrated to be the consequence of C3a binding to these cells. Tritium 0-2 complement C3 Homo sapiens 89-92 16739070-5 2006 [3H]-VBL accumulation is dose-dependent and correlates with the inhibition of Pgp photolabeling affinity, supporting the hypothesis that conferone sensitizes MDCK-MDR1 cells to VBL by competitively inhibiting drug efflux. Tritium 1-3 PGP Canis lupus familiaris 78-81 26160312-9 2015 VPA also significantly decreased the circulating TNF-alpha (p=0.044 vs. LPS group at 3h) and IL-6 protein at 3h (p=0.034 vs. LPS group) and 6h (p=0.026 vs. LPS group) post-treatment. Tritium 109-111 interleukin 6 Canis lupus familiaris 93-97 16739070-6 2006 In MDCK-MDR1 cells, [3H]-VBL accumulation appears to be almost completely dependent on inhibition of Pgp transport. Tritium 21-23 PGP Canis lupus familiaris 101-104 26081760-4 2015 In addition, our biological results indicated that 3d and 3h could suppress RANKL-induced osteoclastogenesis-related marker genes, such as NFATc1, c-fos, TRAP, and cathepsin K. Tritium 58-60 TNF superfamily member 11 Homo sapiens 76-81 848306-2 1977 The time course of the change in 3H-ACh/3H-Ch ratio was linear 0-06 s in all brain regions after administration of 3H-Ch. Tritium 40-42 inversion, Chr X, Harwell 3, proximal Mus musculus 30-35 16604531-4 2006 Cell proliferation in response to CypA was performed by [3H]thymidine incorporation assay. Tritium 57-59 peptidylprolyl isomerase A Homo sapiens 34-38 856647-1 1977 The binding of the glucocorticoid receptor of rat liver to chromatin and DNA has been studied with crude and partially purified preparations of cytosol receptor labelled with [3H]-triamcinolone acetonide in vitro. Tritium 176-180 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 19-42 26081760-4 2015 In addition, our biological results indicated that 3d and 3h could suppress RANKL-induced osteoclastogenesis-related marker genes, such as NFATc1, c-fos, TRAP, and cathepsin K. Tritium 58-60 nuclear factor of activated T cells 1 Homo sapiens 139-145 26081760-4 2015 In addition, our biological results indicated that 3d and 3h could suppress RANKL-induced osteoclastogenesis-related marker genes, such as NFATc1, c-fos, TRAP, and cathepsin K. Tritium 58-60 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 147-152 16495352-4 2006 For this, Madin Darby canine kidney strain II (MDCKII) cells stably expressing human OATP1B3, OATP2B1, or OATP1B1, were assayed for the uptake of 3H-labeled O-methyl-dehydroxymethyl-alpha-amanitin. Tritium 146-148 solute carrier organic anion transporter family member 2B1 Homo sapiens 94-101 25732836-10 2015 The binding sites of 5-HT2A receptors on the plasma membrane of the prefrontal cortex (PFC) were assessed by a receptor-binding assay using tritium-labeled ketanserin, and the density and affinity were calculated from a Scatchard plot. Tritium 140-147 5-hydroxytryptamine (serotonin) receptor 2A Mus musculus 21-27 405334-1 1977 Mitogenic stimulation of pig lymphocytes by anti-immunoglobulin serum and by Con A, PHA, PWM and LPS has been measured by 3H-thymidine incorporation. Tritium 122-124 PHA Sus scrofa 84-87 828056-4 1976 When 3H was used as a label the ratios were much higher than with 14C labelling indicating that the removal of hydrogen at C-9 or C-11 was the rate-limiting step in the biosynthesis of prostaglandin E2 or prostaglandin D2. Tritium 5-7 complement component C9 Oryctolagus cuniculus 123-126 975049-9 1976 The inhibition of DNA synthesis, measured 24 hr after the administration of CCNU and 2 hr after the injection of [3H]thymidine, was almost optimal with 5 mg of CCNU per kg. Tritium 114-116 cyclin O Mus musculus 76-86 25282651-8 2014 As a result of this work, we conclude that compounds 3g and 3h are more potent anticancer agents than VP-16, and that they work by different antitumor mechanisms. Tritium 60-62 host cell factor C1 Homo sapiens 102-107 16495352-4 2006 For this, Madin Darby canine kidney strain II (MDCKII) cells stably expressing human OATP1B3, OATP2B1, or OATP1B1, were assayed for the uptake of 3H-labeled O-methyl-dehydroxymethyl-alpha-amanitin. Tritium 146-148 solute carrier organic anion transporter family member 1B1 Homo sapiens 106-113 24814875-5 2014 Transient overexpression of TSPO enhanced efflux (E%) of [3H]cholesterol to apoA-I, HDL and human serum compared with empty vector (EV) controls, whereas gene knockdown of TSPO achieved the converse. Tritium 58-60 translocator protein Homo sapiens 28-32 24814875-6 2014 Ligation of TSPO (using PK11195, FGIN-1-27 and flunitrazepam) triggered increases in [3H]cholesterol efflux, an effect that was amplified in TSPO-overexpressing macrophages. Tritium 86-88 translocator protein Homo sapiens 12-16 1278179-17 1976 The isolated R-[2H1,3H1]acetate and S-[2H1,3H1]acetate on configurational analysis yielded malates which in the presence of fumarase retained 79.7 +/- 0.7% and 20.3 +/- 0.9%, respectively, of their total tritium content. Tritium 204-211 fumarate hydratase Homo sapiens 124-132 16432504-2 2006 In Tris buffer, [3H]histamine binding to membranes of HEK(hH4R) cells was monophasic (K(D) of 3.8+/-0.8 nM). Tritium 17-19 histamine receptor H4 Homo sapiens 58-62 4454-3 1976 This was accomplished by a direct comparison of the rate of tritium incorporation into position C-2 of histidine 12 of S-peptide (residues 1 to 20) derived from ribonuclease S, with the rates of deuterium exchange of the four histidine C-2 proton resonances of ribonuclease S under the same experimental conditions. Tritium 60-67 complement C2 Bos taurus 96-99 1278159-3 1976 NADPH-specific mitochondrial enoyl-CoA reductase can be assayed by a sensitive radioactive test, employing tritium-labelled NADPH, synthesized in a prefixed reaction from D-[1-3H]-glucose via the hexokinase and glucose-6-phosphate dehydrogenase reactions. Tritium 107-114 glucose-6-phosphate dehydrogenase Homo sapiens 211-244 16432504-4 2006 The inhibition of [3H]histamine binding by two agonists, a protean agonist and five antagonists/inverse agonists confirms that the potency of many H3R ligands is retained or only slightly reduced at the H4R. Tritium 19-21 histamine receptor H3 Homo sapiens 147-150 16278311-9 2006 The selective FAAH inhibitor 1-oxazolo[4,5-b]pyridin-2-yl-1-dodecanone (CAY10435), although having similar efficacy as MAFP in reducing tritium in the thalamus and somatosensory and visual cortices, also reduces caudate putamen and cerebellum (p < 0.01) activity. Tritium 136-143 fatty acid amide hydrolase Mus musculus 14-18 16278311-10 2006 These data indicate FAAH activity generates heterogeneous regional accumulation of [3H]anandamide and metabolites, and they suggest the modulation of endocannabinoid tone by FAAH inhibitors depends upon not only the dose and compound used but also on the degree of FAAH expression in the brain regions examined. Tritium 84-86 fatty acid amide hydrolase Mus musculus 20-24 1194257-3 1975 To determine the stereospecificity with respect to such centers under nonreversal conditions a substrate with a chiral center abC1HH is employed (H = 3H or 2H). Tritium 150-152 ATP binding cassette subfamily A member 1 Homo sapiens 126-130 16278311-10 2006 These data indicate FAAH activity generates heterogeneous regional accumulation of [3H]anandamide and metabolites, and they suggest the modulation of endocannabinoid tone by FAAH inhibitors depends upon not only the dose and compound used but also on the degree of FAAH expression in the brain regions examined. Tritium 84-86 fatty acid amide hydrolase Mus musculus 174-178 16278311-10 2006 These data indicate FAAH activity generates heterogeneous regional accumulation of [3H]anandamide and metabolites, and they suggest the modulation of endocannabinoid tone by FAAH inhibitors depends upon not only the dose and compound used but also on the degree of FAAH expression in the brain regions examined. Tritium 84-86 fatty acid amide hydrolase Mus musculus 174-178 1157447-2 1975 Six patients with advanced cancer were treated with PTG (67 mg/m2 of body surface area intravenously) specifically labeled with tritium as the first dose of a weekly x 6 course. Tritium 128-135 protein phosphatase 1 regulatory subunit 3C Homo sapiens 52-55 16457790-6 2006 In WKY rats, the binding of [3H]-GBR12935 to DAT sites was increased in the basolateral, central and lateral nuclei of the amygdala, lateral nucleus of the hypothalamus, olfactory tubercle, caudate-putamen, nucleus accumbens and substantia nigra (P < 0.05) and decreased in the ventromedial nucleus of the hypothalamus and the CA1 region of the hippocampus. Tritium 29-31 carbonic anhydrase 1 Rattus norvegicus 330-333 16331283-5 2006 In hPAT1 and mPAT1-expressing Xenopus laevis oocytes [3H]GABA uptake was inhibited by vigabatrin, TACA and guvacine, whereas gabapentin failed to inhibit [3H]GABA uptake. Tritium 54-56 solute carrier family 36 member 1 Homo sapiens 3-8 239078-5 1975 The investigations on distribution of 3H-acetyl groups in products of proteolysis of acetylated fibrinogen by thrombin demonstrated a preferential acetylation of fibrinopetide A and absence of radioactive tracer in fibrinopeptide B. Incorporation of the label into fibrinopeptide A opens the possibility for application of fibrinogen labeled with 3H-AcOAc as a convenient substrate for selective investigation on the enzymatic phase of clotting. Tritium 38-40 coagulation factor II, thrombin Bos taurus 110-118 16449797-7 2006 Further, dynorphin (10(-14) M) inhibited substance P (10(-13) M) -induced loss of [3H] DA uptake. Tritium 83-85 tachykinin 1 Mus musculus 41-52 1156576-11 1975 Exhaustive incubation of the tritiated malate (3-H/14-C = 1.95) with fumarase to labilize the pro-R hydrogen at C-3 resulted in release of 65% of the tritium into water. Tritium 150-157 fumarate hydratase Homo sapiens 69-77 1156576-11 1975 Exhaustive incubation of the tritiated malate (3-H/14-C = 1.95) with fumarase to labilize the pro-R hydrogen at C-3 resulted in release of 65% of the tritium into water. Tritium 150-157 complement C3 Homo sapiens 112-115 1156576-14 1975 Selective enrichment of tritium in the pro-R position at C-3 of malate indicates enzymatic carboxylation of pyruvate with retention of configuration in this half-reaction also. Tritium 24-31 complement C3 Homo sapiens 57-60 16386712-7 2006 In both CA1 and CA3 hippocampal subfields, odor stimulation induced an increase of [3H]PDBu binding in both control and EC-lesioned animals, the increase being potentiated only in CA1 of lesioned rats. Tritium 84-86 carbonic anhydrase 1 Rattus norvegicus 8-11 5469-4 1975 Recovery of 75-85% of [3H]-cyclic GMP and a blank of 0.001-0.003% of added [32P]-GTP was routinely obtained. Tritium 23-25 5'-nucleotidase, cytosolic II Homo sapiens 34-37 16107508-2 2006 EGF increased [3H]thymidine and 5-bromo-2"-deoxyuridine incorporation in a time- and dose-dependent manner. Tritium 15-17 epidermal growth factor Mus musculus 0-3 16818375-3 2006 Pharmacological characteristics of A2AR ligands on these cells were evaluated by using [3H]ZM241385- and [35S]GTPgammaS- binding assays. Tritium 88-90 adenosine A2a receptor Homo sapiens 35-39 4129926-0 1973 Synthesis of (3H)DNA complementary to ovalbumin messenger RNA: evidence for limited copies of the ovalbumin gene in chick oviduct. Tritium 14-16 ovalbumin (SERPINB14) Gallus gallus 38-47 5538613-1 1971 Stereospecific distribution of tritium in testosterone-1 alpha,2 alpha-t2. Tritium 31-38 solute carrier family 25 member 5 Homo sapiens 57-73 5275368-1 1970 3-Deoxy-D-arabino-heptulosonic acid 7-phosphate (DAHP) labeled at C-7 randomly or stereospecifically with tritium and at C-1 with (14)C was converted enzymically to 5-dehydroquinate. Tritium 106-113 complement C7 Homo sapiens 66-69 16324696-3 2005 The affinity of MEN16132 for the bradykinin B2 receptor has been investigated by means of competition studies at [3H]bradykinin binding to membranes prepared from Chinese Hamster Ovary (CHO) cells expressing the human bradykinin B2 receptor (pKi 10.5), human lung fibroblasts (pKi 10.5), guinea pig airways (pKi 10.0), guinea pig ileum longitudinal smooth muscle (pKi 10.2), or guinea pig cultured colonic myocytes (pKi 10.3). Tritium 114-116 bradykinin receptor B2 Homo sapiens 44-55 33504296-6 2022 Cannabinoid receptor 1 binding (CB1) was measured with in vitro autoradiography using [3H] SR141716A. Tritium 87-89 cannabinoid receptor 1 Rattus norvegicus 0-22 33504296-6 2022 Cannabinoid receptor 1 binding (CB1) was measured with in vitro autoradiography using [3H] SR141716A. Tritium 87-89 cannabinoid receptor 1 Rattus norvegicus 32-35 16005543-3 2005 Ucn II increased the incorporation of [3H]-leucine into MCs, as well as the accumulation of cAMP and the secretion of atrial natriuretic peptide. Tritium 38-42 urocortin 2 Rattus norvegicus 0-6 32388113-4 2020 Compounds 3a, 3e, 3g, 3h, 3j and 3k showed notable inhibitory effects against hCA I and II. Tritium 22-24 cytochrome P450 family 24 subfamily A member 1 Homo sapiens 78-90 32388113-5 2020 Among these compounds, compound 3h was found to be the most active derivate against both the hCA I and II enzymes with Ki values of 0.032 +- 0.001 muM and 0.013 +- 0.0005 muM, respectively. Tritium 32-34 cytochrome P450 family 24 subfamily A member 1 Homo sapiens 93-105 32388113-7 2020 Furthermore, molecular docking studies were performed to investigate the interaction types between compound 3h and the hCA I and II enzymes. Tritium 108-110 cytochrome P450 family 24 subfamily A member 1 Homo sapiens 119-131 16268637-3 2005 To assess its pharmacokinetic properties in vivo, an efficient synthetic route was developed to introduce a tritium label in position C35 of sangliferin A via an oxidation/reduction strategy. Tritium 108-115 migration and invasion enhancer 1 Homo sapiens 134-137 16081420-4 2005 N-Heptylformamide, a potent inhibitor of alcohol dehydrogenase (ADH), decreased the conversion of [3H]20-HETE to 20-COOH-AA by the MSMC and MEC and also by isolated mouse brain microvessels. Tritium 99-101 alcohol dehydrogenase 1C (class I), gamma polypeptide Homo sapiens 64-67 32126230-7 2020 hCNT1-mediated [3H]-efflux was stimulated by extracellular uridine, but inhibited by extracellular adenosine, with >95% of the radioactivity exiting cells being unmetabolized uridine, consistent with a low transmembrane mobility of the hCNT1/adenosine complex. Tritium 16-18 solute carrier family 28 member 1 Homo sapiens 0-5 32126230-7 2020 hCNT1-mediated [3H]-efflux was stimulated by extracellular uridine, but inhibited by extracellular adenosine, with >95% of the radioactivity exiting cells being unmetabolized uridine, consistent with a low transmembrane mobility of the hCNT1/adenosine complex. Tritium 16-18 solute carrier family 28 member 1 Homo sapiens 236-241 15890711-7 2005 EFS-induced [3H]ACh release from muscle strips significantly (42%) decreased in esophagitis and after 2 h incubation in PGE2 and in PAF C-16. Tritium 13-15 PCNA clamp associated factor Homo sapiens 132-135 32516975-6 2020 Our radioligand binding studies indicated the existence of sigma1Rs as [3H](+)-pentazocine binding sites, and FLV treatment increased the reduced sigma1R maximum binding capacity in TNBS-induced colitis. Tritium 72-74 sigma non-opioid intracellular receptor 1 Rattus norvegicus 59-66 15994442-5 2005 On the chow diet, human apoA-II transgenic mice presented increased [3H]cholesterol in liver (1.3-fold) and feces (6-fold) compared with control mice (P<0.05). Tritium 69-71 apolipoprotein A2 Homo sapiens 24-31 32327488-7 2020 Inhibition of PLD2 activity resulted in prolonged phosphorylation of Tyr-658 in VE-cadherin during the recovery phase 3h post-thrombin challenge. Tritium 118-120 phospholipase D2 Homo sapiens 14-18 15864504-5 2005 When human OAT3 (hOAT3) and hOAT4 were expressed in Xenopus laevis oocytes, only hOAT3 showed [3H]cortisol uptake in excess of that of water-injected control oocytes. Tritium 95-97 solute carrier family 22 member 8 Homo sapiens 11-15 31578541-10 2019 TSPO radioligand binding was increased up to seven times for 3H-PBR28 and up to two times for 3H-PK11195 in active lesions and the centre of chronic active lesions and a strong correlation was found between the radioligand binding signal for both tracers and the number of TSPO+ cells across all of the tissues examined. Tritium 61-63 translocator protein Homo sapiens 0-4 31578541-10 2019 TSPO radioligand binding was increased up to seven times for 3H-PBR28 and up to two times for 3H-PK11195 in active lesions and the centre of chronic active lesions and a strong correlation was found between the radioligand binding signal for both tracers and the number of TSPO+ cells across all of the tissues examined. Tritium 94-96 translocator protein Homo sapiens 0-4 15864504-5 2005 When human OAT3 (hOAT3) and hOAT4 were expressed in Xenopus laevis oocytes, only hOAT3 showed [3H]cortisol uptake in excess of that of water-injected control oocytes. Tritium 95-97 solute carrier family 22 member 8 Homo sapiens 81-86 15368540-13 2005 PLAA peptide decreased [3H]-thymidine incorporation to 50% of the decrease caused by 1alpha,25(OH)2D3. Tritium 24-26 phospholipase A2, activating protein Rattus norvegicus 0-4 15928736-9 2005 In pts with STEMI, peak levels of MCP-1 and MIP-1alpha were significantly higher 3h than 24h from admission (MCP-1 1274.4 vs 1097.4 pg/ml, p<0.02; MIP-1alpha 39.2 vs 22.0 pg/ml, p<0.01). Tritium 81-83 C-C motif chemokine ligand 2 Homo sapiens 34-39 30866091-4 2019 In an animal experimental model of PD, the present study shows that NPY inhibited the activation of microglia evaluated by the binding of the translocator protein (TSPO) ligand [3H]PK11195 in striatum and substantia nigra of 6-OHDA rats. Tritium 178-180 translocator protein Rattus norvegicus 142-162 30866091-4 2019 In an animal experimental model of PD, the present study shows that NPY inhibited the activation of microglia evaluated by the binding of the translocator protein (TSPO) ligand [3H]PK11195 in striatum and substantia nigra of 6-OHDA rats. Tritium 178-180 translocator protein Rattus norvegicus 164-168 31320495-6 2019 VMAT2-mediated [3H]DA uptake inhibition was increased in Taar1 KO mice in synaptosomal and vesicular fractions, but not the membrane-associated fraction, compared with Taar1 WT mice. Tritium 16-18 solute carrier family 18 (vesicular monoamine), member 2 Mus musculus 0-5 24625077-4 2014 METHODS: TSPO binding of DPA-713, DPA-714 and PK11195 were assessed by in vitro competition studies with [3H]DPA-713 using human macrophage THP-1 cells and CD14+ monocytes from healthy volunteers. Tritium 106-108 translocator protein Homo sapiens 9-13 15928736-9 2005 In pts with STEMI, peak levels of MCP-1 and MIP-1alpha were significantly higher 3h than 24h from admission (MCP-1 1274.4 vs 1097.4 pg/ml, p<0.02; MIP-1alpha 39.2 vs 22.0 pg/ml, p<0.01). Tritium 81-83 C-C motif chemokine ligand 3 Homo sapiens 44-54 23578467-7 2013 Significant correlation was shown in c-jun expression values of all patients up to 2 years post transplant between resting state and 3h post-PHA incubation when compared to healthy controls. Tritium 133-135 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 37-42 23578467-8 2013 A positive correlation was found in c-fos gene expression between values at resting state and 3h post PHA incubation in patients with Graft versus Host Disease (GVHD) compared patients with no GVHD or healthy controls. Tritium 94-96 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 36-41 24039778-5 2013 In vitro, quercetin also significantly attenuated Ang II-induced H9C2 cells hypertrophy, as indicated by its concentration dependent inhibitory effects on [3H]leucine incorporation into H9C2 cells (64% reduction) and by the reduced hypertrophic surface area in H9C2 cells compared with the Ang II group (P<0.01, vs. Ang II group). Tritium 156-158 angiogenin Rattus norvegicus 50-53 31460083-5 2019 Complementary high-resolution autoradiographic images using [3H]Ro04-5595 demonstrated strong binding in NR2B receptor-rich regions and low binding in cerebellum where NR2B concentration is low. Tritium 61-63 glutamate ionotropic receptor NMDA type subunit 2B Rattus norvegicus 105-109 30284596-13 2019 Repeated doses of MDPV developed tolerance to this DAT upregulation and 24 h after the 5-day treatment with MDPV, [3H]DA uptake was reduced. Tritium 115-117 solute carrier family 6 member 3 Homo sapiens 51-54 15928736-10 2005 In all STEMI pts there was a positive correlation between MIP-1alpha 3h and left ventricular ejection fraction (LVEF) (r=0.455, p<0.05) and a negative correlation between MIP-1alpha 3h and LV end-diastolic diameter (LVEED) (r=-0.453, p<0.05). Tritium 69-71 C-C motif chemokine ligand 3 Homo sapiens 58-68 23748015-7 2013 The average gammaH2AX foci per cell was significantly elevated 3h after exposure to CSE in cells from Ogg1(-/-)Myh(-/-) double knockout mice compared to wildtype mice. Tritium 63-65 8-oxoguanine DNA-glycosylase 1 Mus musculus 102-106 23748015-7 2013 The average gammaH2AX foci per cell was significantly elevated 3h after exposure to CSE in cells from Ogg1(-/-)Myh(-/-) double knockout mice compared to wildtype mice. Tritium 63-65 mutY DNA glycosylase Mus musculus 111-114 15474986-6 2005 The [3H]-thymidine deoxyribose (TdR) incorporation assay and the comet assay showed that DNA repair was triggered when DNA replication was inhibited after the treatment of 4-NQO, and the hPCNA transactivation seemed to contribute to DNA repair. Tritium 5-7 proliferating cell nuclear antigen Homo sapiens 187-192 30791488-2 2019 This work focused on the characterization of the CAT2 transporter from S. lycopersicum (SlCAT2) that was previously overexpressed in E. coli and reconstituted in proteoliposomes for transport assay as [3H]Arg uptake. Tritium 202-204 catalase isozyme 2 Solanum lycopersicum 49-53 30791488-2 2019 This work focused on the characterization of the CAT2 transporter from S. lycopersicum (SlCAT2) that was previously overexpressed in E. coli and reconstituted in proteoliposomes for transport assay as [3H]Arg uptake. Tritium 202-204 catalase isozyme 2 Solanum lycopersicum 88-94 23971581-0 2013 Measurement of high-pressure shock waves in cryogenic deuterium-tritium ice layered capsule implosions on NIF. Tritium 64-71 S100 calcium binding protein A9 Homo sapiens 106-109 15582352-6 2005 Aryl hydrocarbon receptor (AhR) agonists, beta-naphthoflavone (beta-NF) and polychlorinated biphenyls (PCBs) (10(-6) M) synergistically increased the TCDD-induced inhibition of [(3)H]-thymidine incorporation. Tritium 177-182 aryl hydrocarbon receptor Oryctolagus cuniculus 27-30 23915000-11 2013 Autoradiography and coimmunoprecipitation assays showed [3H]PGZ binding to subunits NDUFA9, NDUFB6, and NDUFA6. Tritium 57-59 NADH:ubiquinone oxidoreductase subunit B6 Mus musculus 92-98 30248432-9 2019 Ex vivo experiments performed in cultured superior cervical ganglia (SCG) cells, rich in NET and OCT3, revealed that mephedrone induces [3H]MPP+ release in an OCT3-dependent manner when NET is fully occupied with MDPV. Tritium 137-139 OCTN3 Homo sapiens 159-163 15503149-6 2004 By measuring the incorporation of tritium-labeled proteins into the PL, we have found that tritium-labeled ZPC is specifically incorporated into the PL. Tritium 34-41 zona pellucida sperm-binding protein 3 Coturnix japonica 107-110 30466968-6 2018 STUDY DESIGN AND METHODS: The affinities of these flavonoids for the BZ-S of GABAA receptors were determined by [3H]flunitrazepam binding to mouse cerebellum membranes in vitro. Tritium 113-115 gamma-aminobutyric acid (GABA) A receptor, subunit gamma 1 Mus musculus 77-82 23542464-6 2013 After 3h bilateral hind-limb ischemia and reperfusion, AMPD3 mRNA, AMPD activity and inosine monophosphate (IMP) increased significantly in WT and AMPD2-deficient mice lungs, while they did not show significant alterations in AMPD3-deficient mice lungs. Tritium 6-8 adenosine monophosphate deaminase 3 Mus musculus 55-60 23542464-7 2013 Genetic inactivation of Ampd3 resulted in markedly accelerated myeloperoxidase (MPO) activity along with exaggerated neutrophils infiltration and hemorrhage in the lungs compared to WT and AMPD2-deficient mice, furthermore, IMP treatment significantly attenuated MPO activity and neutrophils infiltration in WT and the two types of AMPD-deficient mice lungs after 3h reperfusion. Tritium 364-366 adenosine monophosphate deaminase 3 Mus musculus 24-29 15503149-6 2004 By measuring the incorporation of tritium-labeled proteins into the PL, we have found that tritium-labeled ZPC is specifically incorporated into the PL. Tritium 91-98 zona pellucida sperm-binding protein 3 Coturnix japonica 107-110 15469667-5 2004 5-HT1A receptor binding was assayed in post-mortem prefrontal cortex using [3H]8-OH-DPAT, and specific binding determined by 1 microM 5-HT. Tritium 76-78 5-hydroxytryptamine receptor 1A Homo sapiens 0-15 23361422-1 2013 Saturated hydrocarbon mineral oils in vacuum pumps used in 3H handling facilities often contain significant amounts of 3H (as much as several hundred GBq L-1), and during maintenance the air around an open pump may contain MBq L of volatile and aerosol species. Tritium 119-121 ribosomal protein L4 Rattus norvegicus 154-157 30126329-6 2018 We also investigated the effects of haloperidol ( n=9) or placebo ( n=10) on the expression of the translocator protein 18 kDa (TSPO) using autoradiography with [3H]PBR28, a TSPO ligand used in human positron emission tomography (PET) studies. Tritium 162-164 translocator protein Homo sapiens 99-126 30126329-6 2018 We also investigated the effects of haloperidol ( n=9) or placebo ( n=10) on the expression of the translocator protein 18 kDa (TSPO) using autoradiography with [3H]PBR28, a TSPO ligand used in human positron emission tomography (PET) studies. Tritium 162-164 translocator protein Homo sapiens 128-132 15280219-8 2004 PKI166 inhibited the transport of [3H]EG by OATP2. Tritium 35-37 solute carrier organic anion transporter family member 1B1 Homo sapiens 44-49 30276093-6 2018 Linear regression for postoperative values resulted in a CPL of 4 cm corresponding to a TS-CL threshold of 17 . Tritium 88-90 hephaestin Homo sapiens 57-60 23320076-0 2013 Increases in [3H]muscimol and [3H]flumazenil binding in the dorsolateral prefrontal cortex in schizophrenia are linked to alpha4 and gamma2S mRNA levels respectively. Tritium 14-16 immunoglobulin binding protein 1 Homo sapiens 122-128 23123743-5 2013 SRC family of protein tyrosine kinases inhibitor PP2 blocked the total [3H]-inositol phosphate accumulation induced by E2 and PPT in hippocampus, suggesting that ESR1 undergoes translocation from the nuclei to the plasma membrane region via SRC to activate rapid signaling pathways. Tritium 71-75 SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus 0-3 22975130-4 2012 Reactive oxygen species (ROS) in the hippocampal CA3 pyramidal neurons were detected by dihydroethidium (dHEt) at 3h after TBI. Tritium 114-116 carbonic anhydrase 3 Rattus norvegicus 49-52 30028131-2 2018 At elevated solution temperatures, we observe a slow protonation reaction, i.e., [BK+2H]2++H+ [BK+3H]3+, that is regulated by trans cis isomerization of Arg1-Pro2, resulting in the Arg1- cis-Pro2- cis-Pro3-Gly4-Phe5-Ser6- cis-Pro7-Phe8-Arg9 (all- cis) configuration. Tritium 100-102 pyrroline-5-carboxylate reductase 1 Homo sapiens 205-209 30028131-3 2018 Once formed, the all- cis [BK+3H]3+ spontaneously cleaves the bond between Pro2-Pro3 with perfect specificity, a bond that is biologically resistant to cleavage by any human enzyme. Tritium 30-32 pyrroline-5-carboxylate reductase 1 Homo sapiens 80-84 15306230-4 2004 The PKC activator phorbol 12-myristate 13-acetate (PMA; 1 microM) and the PKA activator 8-bromo-cAMP (0.5 mM) also enhanced tritium overflow. Tritium 124-131 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 74-77 29937677-6 2018 Abeta plaque/alpha4beta 2* nAChR dual agent, FA-NIF (GKS-007) exhibited IC50 = 3-6 nM for alpha4beta 2* nAChRs in [3H]cytisine-radiolabeled thalamus and frontal cortex in rat brain slices. Tritium 115-117 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 27-32 22727909-4 2012 Exposure of rats to ozone (2 ppm, 3h) resulted in increased expression of 8-hydroxy-2"-deoxyguanosine (8-OHdG), as well as heme oxygenase-1 (HO-1) in AM. Tritium 34-36 heme oxygenase 1 Rattus norvegicus 123-139 22727909-4 2012 Exposure of rats to ozone (2 ppm, 3h) resulted in increased expression of 8-hydroxy-2"-deoxyguanosine (8-OHdG), as well as heme oxygenase-1 (HO-1) in AM. Tritium 34-36 heme oxygenase 1 Rattus norvegicus 141-145 29530865-4 2018 Using site-directed mutagenesis, generation of chimeric hENTs, and 3H-permeant flux measurements in mutant/chimeric RNA-injected Xenopus oocytes, here we identified the molecular determinants of hENT3 that dictate membrane translocation of 3"-deoxy-nucleoside analogs. Tritium 67-69 solute carrier family 29 member 3 Homo sapiens 195-200 15080796-6 2004 The ability of EL-HDL to mediate 3H-cholesterol efflux from SR-BI (scavenger receptor B type I) overexpressing Chinese-hamster ovary cells was impaired and markedly lower compared with LacZ-HDL at HDL concentrations of 100 microg/ml and above. Tritium 33-35 scavenger receptor class B member 1 Cricetulus griseus 60-65 29217257-2 2018 Ten min, 1h and 3h after the combined chemical and thermal stimulation an up to 2-fold increase in BDNF and Il-1ss protein expression was observed in the lumbar dorsal spinal cord. Tritium 16-18 brain-derived neurotrophic factor Rattus norvegicus 99-103 22297159-10 2012 This result represents the first evidence of an interaction between OT and SERT, as measured by [3H]-Par binding, at peripheral levels in humans. Tritium 97-99 oxytocin/neurophysin I prepropeptide Homo sapiens 68-70 21929836-3 2012 Conversely to NBD-cholesterol, the uptakes of [3H]cholesterol and tocopherol by Caco-2 cells were impaired by both block lipid transport-1 and ezetimibe, which inhibit SR-BI and NPC1L1, respectively. Tritium 46-49 scavenger receptor class B member 1 Homo sapiens 168-173 15080796-6 2004 The ability of EL-HDL to mediate 3H-cholesterol efflux from SR-BI (scavenger receptor B type I) overexpressing Chinese-hamster ovary cells was impaired and markedly lower compared with LacZ-HDL at HDL concentrations of 100 microg/ml and above. Tritium 33-35 scavenger receptor class B member 1 Cricetulus griseus 67-94 21929836-6 2012 [3H]Cholesterol uptake, which correlated closely to NPC1L1 mRNA expression in wild-type (wt) mice, was strongly inhibited by ezetimibe. Tritium 1-3 NPC1 like intracellular cholesterol transporter 1 Mus musculus 52-58 15318115-0 2004 Ethanol-inhibited [3H]thymidine incorporation via protein kinase C-p44/42 mitogen-activated protein kinase/phospholipase A2 signal pathway in renal proximal tubule cells. Tritium 19-21 phospholipase A2 Oryctolagus cuniculus 107-123 29174536-4 2017 Irinotecan and etoposide inhibited [3H]-DAC uptake in negative control small interfering RNA (siRNA)- or dCK siRNA-transfected cells at 10 s, whereas cytarabine and gemcitabine did not. Tritium 36-38 Calcium/calmodulin-dependent protein kinase II Drosophila melanogaster 105-108 28796418-6 2017 By photolabeling experiments performed with a nitrophenylazide containing GM1 oligosaccharide, labeled with tritium, we showed a direct interaction between the GM1 oligosaccharide and the extracellular domain of TrkA receptor. Tritium 108-115 coenzyme Q10A Mus musculus 74-77 28796418-6 2017 By photolabeling experiments performed with a nitrophenylazide containing GM1 oligosaccharide, labeled with tritium, we showed a direct interaction between the GM1 oligosaccharide and the extracellular domain of TrkA receptor. Tritium 108-115 coenzyme Q10A Mus musculus 160-163 22348617-4 2012 All ligands 1-10 displaced the binding of [3H]PK 11195 to the TSPO at nanomolar concentrations, with discrepancies in binding affinity between rat and human TSPO. Tritium 43-45 translocator protein Rattus norvegicus 62-66 15193995-8 2004 We next examined the possible modulation of the binding of the newly available antagonist radioligand for the P2Y(1) receptor, [3H] MRS2279. Tritium 128-130 purinergic receptor P2Y1 Homo sapiens 110-125 22270541-4 2012 beta-AR-stimulation (isoproterenol; 3h) significantly increased expression of ER stress proteins, such as GRP-78, Gadd-153, and Gadd-34, while activating caspase-12 in ARVMs. Tritium 36-38 adrenergic receptor, beta 1 Mus musculus 0-7 22270541-4 2012 beta-AR-stimulation (isoproterenol; 3h) significantly increased expression of ER stress proteins, such as GRP-78, Gadd-153, and Gadd-34, while activating caspase-12 in ARVMs. Tritium 36-38 heat shock protein family A (Hsp70) member 5 Rattus norvegicus 106-112 22270541-4 2012 beta-AR-stimulation (isoproterenol; 3h) significantly increased expression of ER stress proteins, such as GRP-78, Gadd-153, and Gadd-34, while activating caspase-12 in ARVMs. Tritium 36-38 protein phosphatase 1, regulatory subunit 15A Rattus norvegicus 128-135 22270541-4 2012 beta-AR-stimulation (isoproterenol; 3h) significantly increased expression of ER stress proteins, such as GRP-78, Gadd-153, and Gadd-34, while activating caspase-12 in ARVMs. Tritium 36-38 caspase 12 Rattus norvegicus 154-164 28792219-3 2017 Similar to 2, these diarylureas dose-dependently inhibited CP55,940-induced intracellular calcium mobilization and [35S]GTP-gamma-S binding while enhancing [3H]CP55,940 binding to the CB1 receptor. Tritium 157-159 cannabinoid receptor 1 Rattus norvegicus 184-187 15225560-2 2004 This involved competitive binding of the toxin congeners against tritium-labeled STX for receptor sites on rat brain sodium channels. Tritium 65-72 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 Rattus norvegicus 81-84 28806419-13 2017 Neutrophil gelatinase-associated lipocalin [lipocalin-2], a potential regulator of bone homeostasis, was found elevated by 16% at R+3h compared to DI24h. Tritium 132-134 lipocalin 2 Homo sapiens 0-42 28806419-13 2017 Neutrophil gelatinase-associated lipocalin [lipocalin-2], a potential regulator of bone homeostasis, was found elevated by 16% at R+3h compared to DI24h. Tritium 132-134 lipocalin 2 Homo sapiens 44-55 22425184-0 2012 Long-lasting increase in [3H]CP55,940 binding to CB1 receptors following cocaine self-administration and its withdrawal in rats. Tritium 26-28 cannabinoid receptor 1 Rattus norvegicus 49-52 22425184-2 2012 We employed a quantitative autoradiographic analysis using labeled [3H]CP55,940, a CB1 receptor agonist. Tritium 68-70 cannabinoid receptor 1 Rattus norvegicus 83-86 14751540-4 2004 This study was to investigate the mitogenic effect of BK on rabbit CKs linked to activation of p42/p44 mitogen-activated protein kinase (MAPK), assessed by [3H]thymidine incorporation and Western blotting analysis, respectively. Tritium 157-159 cyclin dependent kinase 20 Homo sapiens 95-98 22399138-6 2012 The [3H] cholesterol efflux assay of IMB2026791 treated ABCA1-CHO cells and PMA induced THP-1 macrophages (human acute monocytic leukemia cell) further confirmed the compound as an accelerator of cholesterol efflux in a dose-dependent manner with an EC(50) of 25.23 muM. Tritium 5-7 ATP binding cassette subfamily A member 1 Homo sapiens 56-61 22340895-8 2012 3H-Naloxone binding was elevated in COS-7 and HEK293 cells transfected with gp91phox; in contrast, reduced 3H-naloxone binding was found in neutrophils deficient in gp91phox or in the presence of a NOX2 inhibitor. Tritium 0-2 cytochrome b-245 beta chain Homo sapiens 76-84 22340895-8 2012 3H-Naloxone binding was elevated in COS-7 and HEK293 cells transfected with gp91phox; in contrast, reduced 3H-naloxone binding was found in neutrophils deficient in gp91phox or in the presence of a NOX2 inhibitor. Tritium 0-2 cytochrome b-245 beta chain Homo sapiens 165-173 22340895-8 2012 3H-Naloxone binding was elevated in COS-7 and HEK293 cells transfected with gp91phox; in contrast, reduced 3H-naloxone binding was found in neutrophils deficient in gp91phox or in the presence of a NOX2 inhibitor. Tritium 0-2 cytochrome b-245 beta chain Homo sapiens 198-202 28839429-5 2017 Wild-type and mutant BSEP transport of [3H]-labeled taurocholate (TC) and taurochenodeoxycholate (TCDC) was assessed by vesicular transport assays. Tritium 40-42 ATP binding cassette subfamily B member 11 Homo sapiens 21-25 28595104-4 2017 Plasma pre-beta1 HDL was measured by ELISA, and cholesterol efflux to serum, mediated by ATP-binding cassette transporter A1 (ABCA1), was determined by measuring the transfer of [3H]cholesterol from cultured cells expressing ABCA1 to the medium containing the tested serum. Tritium 179-181 ATP binding cassette subfamily A member 1 Homo sapiens 89-124 28595104-4 2017 Plasma pre-beta1 HDL was measured by ELISA, and cholesterol efflux to serum, mediated by ATP-binding cassette transporter A1 (ABCA1), was determined by measuring the transfer of [3H]cholesterol from cultured cells expressing ABCA1 to the medium containing the tested serum. Tritium 179-181 ATP binding cassette subfamily A member 1 Homo sapiens 126-131 28347675-10 2017 CONCLUSIONS: The Minicare cTnI POC assay may become useful for prompt and safe ruling-out of AMI in ED patients with suspected AMI using a guideline supported 0/3h sampling protocol. Tritium 161-163 troponin I3, cardiac type Homo sapiens 26-30 22227456-8 2012 Alb administration prior to thrombolysis (3h after stroke) can reduce delayed rt-PA treatment-induced brain edema and BBB permeability. Tritium 42-44 albumin Rattus norvegicus 0-3 15047143-7 2004 Prostaglandin J2 and protein C kinase inhibitors significantly inhibited [3H]thymidine incorporation in RMC incubated with VEGF, which was inhibited by TZD. Tritium 74-76 vascular endothelial growth factor A Rattus norvegicus 123-127 21251916-10 2011 The remainder of the [3H]ifenprodil was displaced from the sigma-1 site by the sigma-1 receptor-selective ligand (+)-SKF 10,047. Tritium 22-24 sigma non-opioid intracellular receptor 1 Rattus norvegicus 79-95 28031054-5 2017 The DAT was measured by means of the [3H]-WIN 35,428 binding and the [3H]-DA reuptake to resting lymphocytes membranes. Tritium 38-40 solute carrier family 6 member 3 Homo sapiens 4-7 28031054-5 2017 The DAT was measured by means of the [3H]-WIN 35,428 binding and the [3H]-DA reuptake to resting lymphocytes membranes. Tritium 70-72 solute carrier family 6 member 3 Homo sapiens 4-7 14966990-0 2004 The solution structure of rhombic lanthanide complexes analyzed with a Model-free and crystal-field independent paramagnetic NMR method: application to nonaxial trimetallic complexes [LnxLu(3-x)(TACI-3H)2(H2O)6]3+ (x = 1-3). Tritium 200-202 TNF receptor superfamily member 13B Homo sapiens 195-199 14751456-4 2004 The binding of [3H]LY 278584 to 5-HT(3) receptors was significantly reduced in frontal cortex, CA1 region of hippocampus, and in the medial and lateral nuclei of the amygdala of FH/Wjd versus ACI/N rats. Tritium 16-18 carbonic anhydrase 1 Rattus norvegicus 95-98 28404690-4 2017 Radioligand-binding studies were conducted to assess and compare valbenazine, tetrabenazine, and their respective metabolites in their abilities to selectively and potently inhibit [3H]-HTBZ binding to VMAT2 in rat striatal, rat forebrain, and human platelet homogenates. Tritium 182-184 solute carrier family 18 member A2 Rattus norvegicus 202-207 21753741-6 2011 With HEK293 cells overexpressing GABABR, we showed that clozapine had a significant increase of [3H]-CGP54626A binding at GABABR1 subunit, which provided a clue of the potential therapeutic target of clozapine. Tritium 97-99 gamma-aminobutyric acid type B receptor subunit 1 Homo sapiens 122-129 14736843-4 2004 RT-induced inhibition of [3H]NE release was prevented by synaptosomal entrapment of polyclonal antibodies directed against KCNQ2 subunits, an effect that was abolished by antibody preabsorption with the KCNQ2 immunizing peptide; antibodies against KCNQ3 subunits were ineffective. Tritium 26-28 potassium voltage-gated channel subfamily Q member 2 Rattus norvegicus 123-128 21741637-8 2011 In cells exposed for 10min, 1h and 3h to different amounts of PM(10), transcription of TNFalpha and TRAP1, which code for a key pro-inflammatory cytokine and a mitochondrial protein involved in cell protection from oxidative stress, respectively, was shown to be modulated in a time-dependent, but not a dose-dependent manner. Tritium 35-37 TNF receptor associated protein 1 Homo sapiens 100-105 21168430-7 2011 When HUVECs were incubated in the presence of [3H]DHEA ester-enriched HDL, the amount of intracellular [3H]-radioactivity increased steadily during 24 h. Blocking of SR-B1 reduced this uptake by a mean of 30%. Tritium 47-49 scavenger receptor class B member 1 Homo sapiens 166-171 21168430-7 2011 When HUVECs were incubated in the presence of [3H]DHEA ester-enriched HDL, the amount of intracellular [3H]-radioactivity increased steadily during 24 h. Blocking of SR-B1 reduced this uptake by a mean of 30%. Tritium 104-106 scavenger receptor class B member 1 Homo sapiens 166-171 28315351-0 2017 [3H]BMT-046091 a potent and selective radioligand to determine AAK1 distribution and target engagement. Tritium 1-3 AP2 associated kinase 1 Mus musculus 63-67 28315351-4 2017 We have found that [3H]BMT-046091 is potent and selective AAK1 inhibitor. Tritium 20-22 AP2 associated kinase 1 Mus musculus 58-62 28315351-6 2017 [3H]BMT-046091 binding in the brain is absent in the AAK1 knockout mouse, and is displaceable with a high concentration of AAK1 inhibitors in wild type mice. Tritium 1-3 AP2 associated kinase 1 Mus musculus 53-57 28315351-6 2017 [3H]BMT-046091 binding in the brain is absent in the AAK1 knockout mouse, and is displaceable with a high concentration of AAK1 inhibitors in wild type mice. Tritium 1-3 AP2 associated kinase 1 Mus musculus 123-127 14736843-4 2004 RT-induced inhibition of [3H]NE release was prevented by synaptosomal entrapment of polyclonal antibodies directed against KCNQ2 subunits, an effect that was abolished by antibody preabsorption with the KCNQ2 immunizing peptide; antibodies against KCNQ3 subunits were ineffective. Tritium 26-28 potassium voltage-gated channel subfamily Q member 2 Rattus norvegicus 203-208 28625343-6 2017 Adenosine triphosphate-binding cassette A1 (ABCA1)-mediated cholesterol efflux capacity of HDL was measured using [3H]cholesterol-loaded ABCA1-transfected Chinese hamster ovary cells. Tritium 115-117 phospholipid-transporting ATPase ABCA1 Cricetulus griseus 0-42 14583629-2 2004 The competition studies with these DAGs on [3H]PDBu binding to RasGRP revealed different Ki values for these DAG molecular species. Tritium 44-46 RAS guanyl releasing protein 1 Homo sapiens 63-69 28625343-6 2017 Adenosine triphosphate-binding cassette A1 (ABCA1)-mediated cholesterol efflux capacity of HDL was measured using [3H]cholesterol-loaded ABCA1-transfected Chinese hamster ovary cells. Tritium 115-117 phospholipid-transporting ATPase ABCA1 Cricetulus griseus 44-49 28625343-6 2017 Adenosine triphosphate-binding cassette A1 (ABCA1)-mediated cholesterol efflux capacity of HDL was measured using [3H]cholesterol-loaded ABCA1-transfected Chinese hamster ovary cells. Tritium 115-117 phospholipid-transporting ATPase ABCA1 Cricetulus griseus 137-142 27821594-3 2016 Based upon 3H incorporation into receptor subunits resolved by SDS-PAGE, there was etomidate-inhibitable labeling by [3H]azietomidate in the alpha4 and beta3 subunits and barbiturate-inhibitable labeling by [3H]R-mTFD-MPAB in the beta3 subunit. Tritium 118-120 immunoglobulin binding protein 1 Homo sapiens 141-157 21130085-0 2011 Synthesis and characterization of [3H]-SN56, a novel radioligand for the sigma1 receptor. Tritium 35-37 sigma non-opioid intracellular receptor 1 Rattus norvegicus 73-88 21130085-6 2011 The results indicate that [3H]-SN56 exhibits 1) specific, saturable, and reversible binding to the sigma1 receptor, with B(max)=340+-10 fmol/mg and K(d)=0.069+-0.0074 nM, 2) competitive displacement by classical sigma compounds, yielding sigma1 K(i) values consistent with those reported in the literature, and 3) binding kinetics compatible with a 90 min incubation, and filtration for separation of free and bound radioligand. Tritium 27-29 sigma non-opioid intracellular receptor 1 Rattus norvegicus 99-114 20859661-7 2010 In contrast, [(3)H]verapamil, which is a substrate of P-gp, has a greater RUI value than the lipophilicity trend line, but not for BUI, suggesting that the BRB has an influx transport system for verapamil. Tritium 13-18 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 54-58 21129260-4 2010 The suppression of Treg cells on EL4 cells was detected by 3H-TdR method. Tritium 59-61 epilepsy 4 Mus musculus 33-36 20678474-7 2010 MMP-2 gene expression as well as enzymatic activities of MMP-2 and MMP-9 were increased by IL-1beta (P<0.05 vs. control; 100ng/ml; 3h for gene expression, 48 and 72h for enzymatic activities of MMP-2 and MMP-9, respectively). Tritium 134-136 matrix metallopeptidase 9 Sus scrofa 67-72 27474938-9 2016 In the case of Cyp1b1, mRNA expression remained unchanged compared with the control group following 3h of exposure to TCS, but after 6h, the mRNA expression of Cyp1b1 was decreased. Tritium 100-102 cytochrome P450, family 1, subfamily b, polypeptide 1 Mus musculus 15-21 14750973-4 2004 GluRepsilon1 mutant mice exhibited a malfunction of NMDA receptors, as evidenced by the reduction of [3H]MK-801 binding in an autoradiographic receptor binding assay. Tritium 102-104 glutamate receptor, ionotropic, NMDA2A (epsilon 1) Mus musculus 0-12 27783042-4 2016 Treatment with recombinant TCTP (rTCTP) enhanced both basal and depolarization (50 mM KCl)-evoked [3H]dopamine release in concentration- and time-dependent manners. Tritium 99-101 tumor protein, translationally-controlled 1 Rattus norvegicus 27-31 27483254-5 2016 Here, we show that [3H]dopamine uptake in human (h) MATE1-, hMATE-2K- and mouse (m) MATE-expressing cells exhibited saturable kinetics. Tritium 20-22 solute carrier family 47 member 1 Homo sapiens 52-57 27483254-7 2016 Imatinib, a MATE inhibitor, inhibited [3H]dopamine uptake by hMATE1-, hMATE2-K- and mMATE1-expressing cells in a concentration-dependent manner. Tritium 39-41 solute carrier family 47 member 1 Homo sapiens 61-67 20457138-5 2010 At 3h following LPS injection (4mg/kg body weight), canalicular Mrp2 localization was disrupted without changing the expression of Mrp2 protein or the integrity of tight junctions in the liver. Tritium 3-5 ATP binding cassette subfamily C member 2 Rattus norvegicus 64-68 15365219-3 2004 Therefore, with the present study we aimed to explore the possible existence of NK1 receptors by means of [3H]SR140333 and [125I]BHSP that behave as, respectively, antagonist and agonist, at this level, in human blood lymphocytes and granulocytes of healthy donors. Tritium 107-109 tachykinin receptor 1 Homo sapiens 80-83 20074573-3 2010 We found that [3H]BPA was photo-crosslinked to ND2, ND1 and ND5 subunits, by the three dimensional separation (blue-native/doubled SDS-PAGE) of [3H]BPA-treated bovine heart submitochondrial particles. Tritium 15-17 NADH dehydrogenase subunit 1 Bos taurus 52-55 27483254-8 2016 At clinically-relevant concentrations, imatinib inhibited [3H]dopamine uptake by hMATE1- and hMATE2-K-expressing cells. Tritium 59-61 solute carrier family 47 member 1 Homo sapiens 81-87 20074573-3 2010 We found that [3H]BPA was photo-crosslinked to ND2, ND1 and ND5 subunits, by the three dimensional separation (blue-native/doubled SDS-PAGE) of [3H]BPA-treated bovine heart submitochondrial particles. Tritium 15-17 NADH dehydrogenase subunit 5 Bos taurus 60-63 14638386-5 2003 The experiment compared the accumulation and 3,4-DAP-evoked (or nicotine-evoked) overflow of [3H] in hippocampal slices preincubated with [3H]choline, [3H]noradrenaline, or [3H]serotonin. Tritium 94-96 death-associated protein Rattus norvegicus 49-52 20074573-3 2010 We found that [3H]BPA was photo-crosslinked to ND2, ND1 and ND5 subunits, by the three dimensional separation (blue-native/doubled SDS-PAGE) of [3H]BPA-treated bovine heart submitochondrial particles. Tritium 145-147 NADH dehydrogenase subunit 5 Bos taurus 60-63 20034471-8 2010 Long-term ET-1 stimulation markedly accelerated the rate of ET(A)R degradation and increased the amount of Jab1 bound to ET(A)R with a maximal level of 500% at 3h. Tritium 160-162 COP9 signalosome subunit 5 Homo sapiens 107-111 26809007-8 2016 3H-Chol and MLP cell uptake levels were 4-fold and 9-fold greater in KB HiFR cells when FT-PL-MLP is compared to non-targeted PL-MLP liposomes. Tritium 0-2 cysteine and glycine-rich protein 3 Mus musculus 94-97 26809007-8 2016 3H-Chol and MLP cell uptake levels were 4-fold and 9-fold greater in KB HiFR cells when FT-PL-MLP is compared to non-targeted PL-MLP liposomes. Tritium 0-2 cysteine and glycine-rich protein 3 Mus musculus 94-97 14638386-7 2003 In percent of tissue-[3H], the 3,4-DAP-evoked overflow of [3H]serotonin was increased (+28%), and that of [3H]acetylcholine was reduced (-23%) in the aged rats. Tritium 22-24 death-associated protein Rattus norvegicus 35-38 26743504-6 2016 IMD 1nmol L(-1), applied either throughout ischaemia (3h) and re-oxygenation (1h) or during re-oxygenation (1h) alone, attenuated HCM injury (P<0.05); cell viabilities were 59% and 61% respectively vs. 39% in absence of IMD. Tritium 54-56 Bruton tyrosine kinase Homo sapiens 0-5 14638386-7 2003 In percent of tissue-[3H], the 3,4-DAP-evoked overflow of [3H]serotonin was increased (+28%), and that of [3H]acetylcholine was reduced (-23%) in the aged rats. Tritium 59-61 death-associated protein Rattus norvegicus 35-38 20814156-7 2010 Like uridine uptake, [3H]D-glucose uptake by ROVs, which is mediated by facilitative glucose transporter GLUT1, was trans-stimulated by unlabeled D-glucose at ICT, and the overshoot phenomenon was observed. Tritium 22-24 solute carrier family 2 member 1 Homo sapiens 105-110 14638386-7 2003 In percent of tissue-[3H], the 3,4-DAP-evoked overflow of [3H]serotonin was increased (+28%), and that of [3H]acetylcholine was reduced (-23%) in the aged rats. Tritium 59-61 death-associated protein Rattus norvegicus 35-38 14660011-4 2003 [3H]Naltrindole bound specifically to a single high-affinity site that was displaced by delta-opioid receptor ligands with the rank order of affinity: naltriben >7-benzylidenenaltrexone>deltorphin II>[D-Pen(2), D-Pen(5)] enkephalin. Tritium 1-3 opioid receptor delta 1 Sus scrofa 88-109 21088444-7 2010 In cells transfected with a specific peroxisome proliferator-activated receptor (PPAR)-alpha small interfering RNA, the induction of ABCA1 expression and apoA-I-mediated 3H-cholesterol efflux by aspirin were substantially suppressed. Tritium 170-172 apolipoprotein A-I Mus musculus 154-160 19679388-4 2009 Using western blot analysis 3h after ESWT, an increased expression of Bax was found, indicating a fast pro-apoptotic effect of treatment on some of the osteoblasts. Tritium 28-30 BCL2-associated X protein Mus musculus 70-73 19722701-11 2009 Likewise, PappA-B of [3H]-TPP was 5-fold greater in P-gp knockout mice than in controls. Tritium 22-24 phosphoglycolate phosphatase Mus musculus 52-56 19789505-3 2009 MATERIAL/METHODS: We investigated MAO labeling with mechanism-based inhibitor [3H]pargyline activities of MAO A, MAO B, and SSAO in healthy and inflamed human dental pulp. Tritium 79-81 monoamine oxidase B Homo sapiens 113-118 26320080-3 2015 When hippocampal slices were incubated in 3-30muM SEL for 3h, but not 30 min, the relative amplitude of the CA1 field excitatory synaptic potentials decreased over the course of a train of high frequency stimuli more than for control slices. Tritium 58-60 carbonic anhydrase 1 Homo sapiens 108-111 25739102-6 2015 mAKAPbeta-shRNA was constructed for RNA interference; the expression of mAKAPbeta and hypertrophic markers, the cell surface area and the [3H]Leucine incorporation rate in the AngII-treated rat cardiomyocytes were detected following RNA interference. Tritium 139-141 A kinase (PRKA) anchor protein 6 Mus musculus 0-9 25697061-3 2015 Exposure to cold-restraint stress for 3h or subcutaneous infusion of N/OFQ, 1 mug/kg/h for 4h, induced a significant increase of the area of synaptophysin-immunoreactive nerve fibers in the fundic mucosa, while prolonged subcutaneous infusion of N/OFQ, 1 mug/kg/h for 52 h and for 14 days, did not modify the synaptophysin-immunostained fibers. Tritium 38-40 synaptophysin Rattus norvegicus 141-154 25091564-10 2014 We found that autophagy induction in HeLa cells by Tat-beclin 1 peptide 3h after WNV inoculation inhibited viral replication, and GFP expression was significantly inhibited in wild-type cells when compared with cells lacking Atg5. Tritium 72-74 beclin 1 Homo sapiens 55-63 14660011-4 2003 [3H]Naltrindole bound specifically to a single high-affinity site that was displaced by delta-opioid receptor ligands with the rank order of affinity: naltriben >7-benzylidenenaltrexone>deltorphin II>[D-Pen(2), D-Pen(5)] enkephalin. Tritium 1-3 proenkephalin Sus scrofa 230-240 14660012-2 2003 Exposure (48 and/or 96 h) of human embryonic kidney (HEK 293) cells stably expressing recombinant alpha1beta2gamma2s GABA(A) receptors for gamma-aminobutyric (GABA, 1 mM) and muscimol (100 microM), but not for diazepam (1 microM), enhanced the maximum number (B(max)) of [3H]flunitrazepam binding sites without affecting their affinity (K(d)). Tritium 272-274 adrenoceptor alpha 1D Homo sapiens 98-115 19197005-0 2009 Cannabinoid CB1 and cholecystokinin CCK2 receptors modulate, in an opposing way, electrically evoked [3H]GABA efflux from rat cerebral cortex cell cultures: possible relevance for cortical GABA transmission and anxiety. Tritium 102-104 cannabinoid receptor 1 Rattus norvegicus 12-15 24141017-7 2014 These cellular events were followed by enhanced upregulation of NOS II and peroxynitrite expression 3h after sustained seizure activity that led to an increase of caspase-3 and DNA fragmentation in the hippocampal CA3 neurons 7days after experimental status epilepticus. Tritium 100-102 carbonic anhydrase 3 Rattus norvegicus 214-217 14624766-7 2003 In the presence of ECM, IGFBP-2/IGF-II was as effective as IGF-II alone in stimulating [3H]thymidine and [3H]proline incorporation and in inhibiting apoptosis in cultured human osteoblasts. Tritium 88-90 insulin like growth factor 2 Homo sapiens 32-38 23990661-8 2013 CEH and SR-BI expression enhanced the movement of [3H]label from HDL-[3H]CE to bile acids in vitro and in vivo. Tritium 51-53 scavenger receptor class B member 1 Homo sapiens 8-13 14624766-7 2003 In the presence of ECM, IGFBP-2/IGF-II was as effective as IGF-II alone in stimulating [3H]thymidine and [3H]proline incorporation and in inhibiting apoptosis in cultured human osteoblasts. Tritium 106-108 insulin like growth factor 2 Homo sapiens 32-38 23990661-8 2013 CEH and SR-BI expression enhanced the movement of [3H]label from HDL-[3H]CE to bile acids in vitro and in vivo. Tritium 70-72 scavenger receptor class B member 1 Homo sapiens 8-13 19257909-7 2009 The effects of Id2 and its mutants on cell proliferation were determined by [3H]-thymidine incorporation assay and the 3- [4, 5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) dye method. Tritium 77-79 inhibitor of DNA binding 2 Homo sapiens 15-18 14624766-7 2003 In the presence of ECM, IGFBP-2/IGF-II was as effective as IGF-II alone in stimulating [3H]thymidine and [3H]proline incorporation and in inhibiting apoptosis in cultured human osteoblasts. Tritium 106-108 insulin like growth factor 2 Homo sapiens 59-65 14534366-4 2003 Dissociation of [3H][Dmt1]DALDA was also rapid (K(-1) = 0.032 min(-1)) and indicated binding to a single site. Tritium 17-19 doublesex and mab-3 related transcription factor 1 Homo sapiens 21-25 14534366-5 2003 [3H][Dmt1]DALDA binds with very high affinity to human mu opioid receptor (hMOR) (Kd = 0.199 nM), and Kd and Bmax were reduced by sodium but not Gpp(NH)p [guanosine 5"-(beta,gamma-imido)triphosphate]. Tritium 1-3 doublesex and mab-3 related transcription factor 1 Homo sapiens 5-9 19158435-6 2009 Our investigations of the in vivo biodistribution of tritium-labelled LPYFDa and single-unit electrophysiology revealed that LPYFDa readily crosses the blood-brain barrier, and protects the synapses against the excitatory action of fibrillar Abeta (1-42) in a relatively wide temporal window in rat. Tritium 53-60 amyloid beta precursor protein Rattus norvegicus 242-247 24204775-6 2013 High-fat diet- fed PTP1B knockout mice also exhibited improved glucose uptake measured using [(3)H]-2-deoxy-glucose incorporation assay and Akt phosphorylation in the skeletal muscle tissue, compared to their wild-type control mice which received similar diet. Tritium 93-98 protein tyrosine phosphatase, non-receptor type 1 Mus musculus 19-24 14534366-9 2003 The Ki values for a number of opioid ligands were generally higher when determined by competitive displacement binding against [3H][Dmt1]DALDA compared with [3H]DAMGO, with the exception of Dmt1-substituted peptide analogs. Tritium 128-130 doublesex and mab-3 related transcription factor 1 Homo sapiens 132-136 14534366-12 2003 The very high affinity and selectivity of [3H][Dmt1]DALDA for the mu receptor, together with its very low nonspecific binding (10-15%) and metabolic stability, make [3H][Dmt1]DALDA an ideal radioligand for labeling mu receptors. Tritium 43-45 doublesex and mab-3 related transcription factor 1 Homo sapiens 47-51 14534366-12 2003 The very high affinity and selectivity of [3H][Dmt1]DALDA for the mu receptor, together with its very low nonspecific binding (10-15%) and metabolic stability, make [3H][Dmt1]DALDA an ideal radioligand for labeling mu receptors. Tritium 43-45 doublesex and mab-3 related transcription factor 1 Homo sapiens 170-174 24224084-7 2013 At doses that lowered body weight, AngII significantly increased ISBAT [3H]NIS binding density. Tritium 72-74 solute carrier family 10 member 2 Rattus norvegicus 65-70 18708079-4 2008 Subsequently, CB1 receptor number and function were assessed by [3H]SR141716 and WIN55,212-2-stimulated [35S]GTPgammaS binding, respectively. Tritium 65-67 cannabinoid receptor 1 Rattus norvegicus 14-17 14534366-12 2003 The very high affinity and selectivity of [3H][Dmt1]DALDA for the mu receptor, together with its very low nonspecific binding (10-15%) and metabolic stability, make [3H][Dmt1]DALDA an ideal radioligand for labeling mu receptors. Tritium 166-168 doublesex and mab-3 related transcription factor 1 Homo sapiens 47-51 19009649-7 2008 RESULTS: Stable c-Fos overexpression increased cell proliferation under low serum conditions and resulted in a two-fold increase in [3H]-thymidine incorporation following serum addition. Tritium 133-135 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 16-21 23762418-8 2013 P-gp activity was assessed by [3H]digoxin accumulation. Tritium 31-33 phosphoglycolate phosphatase Mus musculus 0-4 23430614-7 2013 In mouse aortic smooth muscle cells (MASM), we found that the growth rate and [3H]-thymidine incorporation of the GIT1 KO MASM were significantly decreased compared with the wild-type MASM. Tritium 79-81 GIT ArfGAP 1 Mus musculus 114-118 18806692-4 2008 Successful TSPO knockdown was characterized by reduction of 20% in TSPO RNA levels, 50% in protein expression of the TSPO, and 50% in binding with the TSPO ligand, [3H]PK 11195. Tritium 165-167 translocator protein Homo sapiens 11-15 14534366-12 2003 The very high affinity and selectivity of [3H][Dmt1]DALDA for the mu receptor, together with its very low nonspecific binding (10-15%) and metabolic stability, make [3H][Dmt1]DALDA an ideal radioligand for labeling mu receptors. Tritium 166-168 doublesex and mab-3 related transcription factor 1 Homo sapiens 170-174 14637376-5 2003 Saturation analysis revealed [3H]EBOB binding at a single site, with a K(d) of approximately 9 nM in alpha1beta2gamma2 and alpha2beta2gamma2 receptors. Tritium 30-32 adrenoceptor alpha 1D Homo sapiens 101-118 18790019-8 2008 The data obtained show that Abeta(1-40) peptide decreased astroglial glutamate uptake capacity in a non-competitive mode of inhibition, assessed in terms of tritium radiolabeled d-aspartate (d-[(3)H]aspartate) transport. Tritium 157-164 amyloid beta precursor protein Rattus norvegicus 28-33 23207410-4 2013 Moreover, partial recovery of MAO-B activity was observed after repeated washing in the presence of isatin (reversible inhibitor) and compounds 3g and 3h suggesting a reversible inhibition of the enzyme. Tritium 151-153 monoamine oxidase B Homo sapiens 30-35 22981453-10 2012 The increase in electrically induced [(3)H]ACh release produced by 0.01muM CCK-8 was antagonized by d,l loxiglumide (CR 1505), 10muM, a non-peptide CCK-A receptor antagonist and by Suc-Tyr-(OSO3)-Met-Gly-Trp-Met-Asp-beta-phenethyl-amide (GE-410), 1muM, a peptide CCK-A receptor antagonist. Tritium 38-42 cholecystokinin Rattus norvegicus 75-78 14637376-9 2003 Overall, our data demonstrate that [3H]EBOB binds to a single, high affinity site in alpha1beta2gamma2 and alpha2beta2gamma2 GABAA receptors, and modulation of its binding is similar to that seen with [35S]TBPS. Tritium 36-38 adrenoceptor alpha 1D Homo sapiens 85-102 18597894-4 2008 In the present work using a [(3)H]-labeled derivative of the heptapeptide we have identified and characterized [(3)H]-SP(1-7) binding in the rat ventral tegmental area (VTA). Tritium 29-33 Sp1 transcription factor Rattus norvegicus 118-124 14656321-5 2003 Autoradiography reveals a similar spatial distribution of [3H]GHB-binding sites in brains of GABAB(1)-/- and wild-type mice. Tritium 59-61 gamma-aminobutyric acid (GABA) B receptor, 1 Mus musculus 93-104 18672288-7 2008 In contrast, reconstituted RAG-1-/- mice that underwent 40min intestinal ischemia and 3h reperfusion showed significant injury in the local tissues. Tritium 86-88 recombination activating 1 Mus musculus 27-32 22753155-4 2012 Moreover, the cytotoxic effect of the most potent compound 3h on HUH7 and MCF7 cells through apoptosis was visualized by Hoechst staining and compared with paclitaxel, which is a mitotic inhibitor acting on microtubules. Tritium 59-61 MIR7-3 host gene Homo sapiens 65-69 14606065-2 2003 METHODS: Recombinant adenovirus Ad-p27kip1 was constructed and transfected into esophageal carcinoma cell EC-9706, and its effect on p27kip1 expression, the growth of esophageal carcinoma cell, DNA replication, protein synthesis, cell multiplication and apoptosis were explored by means of cell growth count, 3H-TdR, 3H-Leucine incorporation, flow cytometry, DNA fragment analysis and TUNEL. Tritium 309-311 cyclin dependent kinase inhibitor 1B Homo sapiens 35-42 21896291-0 2011 Neurotensin decreases high affinity [3H]-ouabain binding to cerebral cortex membranes. Tritium 37-39 neurotensin Rattus norvegicus 0-11 14606065-2 2003 METHODS: Recombinant adenovirus Ad-p27kip1 was constructed and transfected into esophageal carcinoma cell EC-9706, and its effect on p27kip1 expression, the growth of esophageal carcinoma cell, DNA replication, protein synthesis, cell multiplication and apoptosis were explored by means of cell growth count, 3H-TdR, 3H-Leucine incorporation, flow cytometry, DNA fragment analysis and TUNEL. Tritium 317-319 cyclin dependent kinase inhibitor 1B Homo sapiens 35-42 21813204-7 2011 Exposure of splenocytes to AEBSF for 3h noticeably inhibited the induction of IFNgamma, IL-12, and IL-12-induced STAT4beta, mRNA expression of T-bet and IL-12Rbeta2. Tritium 37-39 T-box 21 Mus musculus 143-148 18497332-3 2008 In support of this hypothesis, when [3H]taurocholic acid or [3H]estrone 3-sulfate were administered into the ileal lumen, absorption was lower in Ostalpha-/- mice. Tritium 36-40 solute carrier family 51, alpha subunit Mus musculus 146-154 14535785-0 2003 Synthesis of a tritium-labeled, fipronil-based, highly potent, photoaffinity probe for the GABA receptor. Tritium 15-22 GABA type A receptor-associated protein Homo sapiens 91-104 19160959-5 2008 The inhibitory role of the CD4+CD25+ T cells was assessed by [3H] thymidine incorporation method and the cytokines in the cultural supernatant were detected by ELISA. Tritium 62-64 CD4 antigen Mus musculus 27-30 21685551-4 2011 NS3 protease sequences of HCV-3h recovered in the present study harbour specific amino acid residues not encountered in other reference HCV genotypes and subtypes at nine of the 181 NS3 protease positions; none of these amino acids are known to confer resistance to PIs. Tritium 30-32 KRAS proto-oncogene, GTPase Homo sapiens 0-3 14511674-6 2003 Co-treatment with isoflavones, curcumin and tetrahydrocurcumin, increased [3H]EGCG accumulation significantly in MDCKII/MRP1 and HT-29 cells. Tritium 75-77 ATP binding cassette subfamily C member 1 Canis lupus familiaris 120-124 21685551-4 2011 NS3 protease sequences of HCV-3h recovered in the present study harbour specific amino acid residues not encountered in other reference HCV genotypes and subtypes at nine of the 181 NS3 protease positions; none of these amino acids are known to confer resistance to PIs. Tritium 30-32 KRAS proto-oncogene, GTPase Homo sapiens 182-185 20932595-4 2011 RESULTS: We confirmed that a tritium-labeled compound analog (ITX 7650) binds cells expressing SR-BI, and both ITX 5061 and ITX 7650 compete for HDL-mediated lipid transfer in an SR-BI dependent manner. Tritium 29-36 scavenger receptor class B member 1 Homo sapiens 95-100 17823201-7 2008 Proliferation of TSK/+ B cells and fibroblasts to anti-CD40 antibodies was assessed by the uptake of [3H]-labelled thymidine and bromodeoxyuridine, respectively. Tritium 102-104 fibrillin 1 Mus musculus 17-20 12932673-1 2003 Two bona fide c-Src inhibitors, denominated CGP77675 and CGP76030, reduced in a time- and concentration-dependent manner (i) the proliferation of the PC3 prostate carcinoma cell line, as assessed by the [3H]-thymidine incorporation test, (ii) the capacity of PC3 cells to adhere and spread on Matrigel substrate, as determined by crystal violet staining, (iii) the ability of PC3 cells to migrate through a gelatine boundary and invade a Matrigel substrate. Tritium 204-206 proprotein convertase subtilisin/kexin type 1 Homo sapiens 150-153 18520071-5 2008 Multidrug resistance-associated protein (MRP) 4 substrates/inhibitors including methotrexate, indomethacin, taurocholic acid and indocyanine green significantly inhibited [3H]PGE1 uptake. Tritium 172-174 ATP binding cassette subfamily C member 4 Rattus norvegicus 0-47 18393501-4 2008 Collision-induced dissociation of the [OT - 3H + Cu](-) complex yielded exclusively C-terminal Cu(2+)-containing fragments (Cu(2+)fragment(3-)), suggesting that the Cu(2+) ligation site includes deprotonated C-terminal backbone amide nitrogen atoms and the N-terminal amino nitrogen atom in [OT - 3H + Cu](-). Tritium 44-46 oxytocin/neurophysin I prepropeptide Homo sapiens 39-41 18310473-6 2008 In transfected cells, TAAR1 in response to dopamine, norepinephrine, and serotonin significantly inhibited uptake and promoted efflux of [3H]dopamine, [3H]norepinephrine, and [3H]serotonin, respectively, whereas the monoamine autoreceptors, D2s, alpha(2A), and 5-HT(1B) enhanced the uptake function under the same condition. Tritium 138-140 trace amine associated receptor 1 Homo sapiens 22-27 18310473-6 2008 In transfected cells, TAAR1 in response to dopamine, norepinephrine, and serotonin significantly inhibited uptake and promoted efflux of [3H]dopamine, [3H]norepinephrine, and [3H]serotonin, respectively, whereas the monoamine autoreceptors, D2s, alpha(2A), and 5-HT(1B) enhanced the uptake function under the same condition. Tritium 152-154 trace amine associated receptor 1 Homo sapiens 22-27 18310473-6 2008 In transfected cells, TAAR1 in response to dopamine, norepinephrine, and serotonin significantly inhibited uptake and promoted efflux of [3H]dopamine, [3H]norepinephrine, and [3H]serotonin, respectively, whereas the monoamine autoreceptors, D2s, alpha(2A), and 5-HT(1B) enhanced the uptake function under the same condition. Tritium 152-154 trace amine associated receptor 1 Homo sapiens 22-27 21731704-9 2011 The present findings suggest that [3H]CHIBA-3007 would be a useful new radioligand for studying GlyT-1 in the brain. Tritium 35-37 solute carrier family 6 member 9 Rattus norvegicus 96-102 21143913-10 2010 C/EBP-delta siRNA treatment resulted in defective growth arrest as demonstrated by persistently elevated BrdU labeling, 3H-thymidine incorporation and cyclin D1 levels in response to growth arrest treatments. Tritium 120-122 CCAAT enhancer binding protein delta Homo sapiens 0-11 21080372-5 2010 In addition, the measured methylation level of cellular proteins using a tritium-labeled methyl donor was accordingly enhanced upon ectopic expression of PRMT1. Tritium 73-80 protein arginine methyltransferase 1 Homo sapiens 154-159 20083572-6 2010 The effect of StAR overexpression on apoAI-dependent [3H]cholesterol efflux was mimicked by non-sterol agonist, T901317, and 27-hydroxycholesterol, and blocked by LXR inhibitor, geranylgeranyl pyrophosphate, sterol 27-hydroxylase inhibitor, GW273297x, and probucol, inhibitor of ATP binding cassette transporter A1 (ABCA1). Tritium 54-56 ATP-binding cassette, sub-family A (ABC1), member 1 Mus musculus 279-314 12950637-9 2003 Results of 3H-thymidine incorporation assays showed a significant reduction in proliferation of the mononuclear cells cocultured with IDO-expressing skin cells compared to monocytes cocultured with control (non-IDO-expressing) skin cells (p < 0.001). Tritium 11-13 indoleamine 2,3-dioxygenase 1 Homo sapiens 134-137 20083572-6 2010 The effect of StAR overexpression on apoAI-dependent [3H]cholesterol efflux was mimicked by non-sterol agonist, T901317, and 27-hydroxycholesterol, and blocked by LXR inhibitor, geranylgeranyl pyrophosphate, sterol 27-hydroxylase inhibitor, GW273297x, and probucol, inhibitor of ATP binding cassette transporter A1 (ABCA1). Tritium 54-56 ATP-binding cassette, sub-family A (ABC1), member 1 Mus musculus 316-321 17625500-8 2008 Post-mortem analysis of METH brains showed 20% lower striatal DA content while autoradiography studies of precommissural striatum showed 35% lower [3H]WIN35428 binding to the DA transporter. Tritium 148-150 solute carrier family 6 member 3 Homo sapiens 175-189 12906759-6 2003 Moreover, the inhibitory effects of various compounds, including some peptides, on [(3)H]estrone-3-sulfate uptake by OATP-C were similar to those observed in [(3)H]DADLE uptake by rat isolated hepatocytes. Tritium 85-88 solute carrier organic anion transporter family member 1B1 Homo sapiens 117-123 18336811-3 2008 LH-21 inhibited the binding of [3H]CP55940 to cloned human and rat CB1 receptors with IC50 values of 631+/-98 nM, and 690+/-41 nM, respectively, and acted as an inverse agonist in a cAMP functional assay using cultured cells expressing human, rat or mouse CB1 receptor. Tritium 32-34 cannabinoid receptor 1 Rattus norvegicus 67-70 18374569-3 2008 Replacement of the (2-aryl)-ethynyl-moiety in 8-position with smaller less lipophilic substituents produced compounds inhibiting the binding of [3H]-LY354740 to rat mGluR2 with low nanomolar affinity and consistent functional effect at both mGluR2 and mGluR3. Tritium 145-147 glutamate receptor, ionotropic, AMPA3 (alpha 3) Mus musculus 252-258 19799993-12 2010 While the increase in the expression of cyclin D1 was observed when retinal explants were incubated for 3h or longer periods of time, the decrease in the expression of p27(kip1) was noticed only after 6h incubation with ATP. Tritium 104-106 cyclin D1 Mus musculus 40-49 20022380-8 2010 Interestingly, IL-8 and ICAM-1 had a modest effect on neutrophil TEM in this 3h assay which was significantly diminished by the inhibition of Rho kinase in HUVECs with Y27632. Tritium 77-79 intercellular adhesion molecule 1 Homo sapiens 24-30 12626648-8 2003 Phe-153-->Leu-153 or Ile-433-->Val-433 exchange in hH1R (hH1R-->gpH1R) resulted in poor receptor expression, low [3H]mepyramine affinity, and functional inactivity. Tritium 123-125 histamine receptor H1 Homo sapiens 57-61 18096134-12 2008 After stimulation with motilin and EM-A the t(1/2) for MTLR resensitization was 3h and 1h, respectively but amounted 26h for ABT-229. Tritium 80-82 Mlnr Cricetulus griseus 55-59 12626648-8 2003 Phe-153-->Leu-153 or Ile-433-->Val-433 exchange in hH1R (hH1R-->gpH1R) resulted in poor receptor expression, low [3H]mepyramine affinity, and functional inactivity. Tritium 123-125 histamine receptor H1 Homo sapiens 63-67 19910055-1 2010 The aim of our study was to investigate and compare the dopamine (DA) transporter (DAT) in resting lymphocytes of 20 psychotic patients and 20 healthy control subjects, by means of both the binding parameters (Bmax and Kd) of 3H-WIN 35,428, and the reuptake parameters (Vmax and Km) of 3H-DA. Tritium 226-228 solute carrier family 6 member 3 Homo sapiens 83-86 18180165-4 2008 Primary cultures were incubated with [3H]lyso-PAF and stimulated by TNFalpha in the presence or absence of wortmannin. Tritium 38-40 PCNA clamp associated factor Rattus norvegicus 46-49 12752772-9 2003 3H-Substance P autoradiography revealed a good correlation between receptor binding sites and NK1 mRNA expression throughout the brain, with the highest levels of binding in the locus coeruleus. Tritium 0-2 tachykinin receptor 1 Homo sapiens 94-97 17602283-3 2008 The interaction of PDZK1 and NHERF1 with hOAT4 and hOAT4-Delta was investigated by measurement of [3H] estrone sulfate uptake, cell surface and total cell expression of hOAT4. Tritium 99-101 PDZ domain containing 1 Homo sapiens 19-24 19628755-8 2009 Instead, roscovitine enhanced spontaneous [3H]DA outflow and inhibited DAT-mediated [3H]DA reaccumulation into dSTR slices. Tritium 85-87 solute carrier family 6 member 3 Homo sapiens 71-74 12679720-5 2003 rOAT3-expressing oocytes exhibited [3H]HVA uptake (K(m) = 274 micromol/L), which was inhibited by several organic anions, such as PAH, indoxyl sulfate, octanoic acid, and metabolites of monoamine neurotransmitters. Tritium 36-38 solute carrier family 22 member 8 Rattus norvegicus 0-5 19233134-3 2009 [3H]palmitic acid labeling of SMS1 or SMS2-overexpressing HEK293 cells revealed that SMS2, but not SMS1, is palmitoylated. Tritium 1-3 sphingomyelin synthase 1 Homo sapiens 30-34 19098122-8 2009 For T62A-hDAT, however, the measured 50% reduction in both [(3)H]dopamine uptake and [(3)H]dopamine efflux was consistent with a slowed transition between inward- and outward-facing conformations. Tritium 61-64 solute carrier family 6 member 3 Homo sapiens 9-13 18690876-4 2008 In our previous studies we investigated the presence of nuclear PBR in different hepatic tumour cell lines with regard to binding to [3H] PK 11,195 and protein analysis. Tritium 134-136 translocator protein Homo sapiens 64-67 18083057-5 2008 The presence of nicotinic receptors containing alpha4(beta2) and alpha7 subunits in the bone marrow cells of C57Bl/6 mice was shown by the binding of [125I]-alpha-bungarotoxin or [3H]-Epibatidine and by flow cytometry with subunit-specific antibodies or fluorescein-labeled alpha-cobratoxin. Tritium 180-182 hemoglobin, beta adult minor chain Mus musculus 16-59 12527113-4 2003 The affinity of Tau proteins for WGA lectin, together with evidence from [3H]-galactose transfer and analysis of beta-eliminated products, demonstrated the presence of O-GlcNAc residues on both cytosolic and nuclear Tau proteins. Tritium 74-76 O-linked N-acetylglucosamine (GlcNAc) transferase Homo sapiens 168-176 18369695-7 2008 Moreover, both VEGF-induced [3H]-thymidine uptake and the migration of BRECs were significantly inhibited in the presence of fasudil. Tritium 29-31 vascular endothelial growth factor A Mus musculus 15-19 19239183-11 2009 The uptake of [3H]taurine in ST2 cells treated with 4 mM calcium was increased 1.7-fold. Tritium 15-17 interleukin 1 receptor-like 1 Mus musculus 29-32 12628298-6 2002 Heterologous expression of pOAT1 in Xenopus laevis oocytes revealed an apparent K(m) for [3H]PAH of 3.75 +/- 1.6 microM. Tritium 90-92 solute carrier family 22 member 6 Sus scrofa 27-32 19178046-4 2008 The present work is the study of the mutation frequency at MCS/MNS loci in 19 families of workers occupationally exposed to chronic beta-radiation from tritium and tritium oxide (examined group), and in the control group included 23 families. Tritium 152-159 Miles-Carpenter X-linked mental retardation syndrome Homo sapiens 59-62 17964527-12 2007 Long-lived and very-long-lived protein degradation was reduced in the cells with reduced IDE expression (14.0+/-0.16 vs. 12.5+/-0.07%/4h (long-lived), 9.6+/-2.2% vs. 7.3+/-0.2%/3h (very-long-lived), control vs. IDE transfected, respectively, P<0.005). Tritium 177-179 insulin degrading enzyme Homo sapiens 89-92 12628298-7 2002 [3H]PAH uptake mediated by pOAT1 was abolished by 0.5 mM glutarate or 1 mM probenecid. Tritium 1-3 solute carrier family 22 member 6 Sus scrofa 27-32 12127559-3 2002 Abundant expression of IGF-I, IGF-II and IGF-IR was demonstrated in resting cells and exogenous IGF-I and IGF-II increased 3H-thymidine incorporation in a dose dependent manner. Tritium 123-125 insulin like growth factor 2 Homo sapiens 106-112 17166497-7 2007 Furthermore, 100 nM of R-154716 completely inhibited [(3)H]COE-labeled HDL uptake induced by overexpression of scavenger receptor BI (SR-BI) in HEK293 cells. Tritium 54-58 scavenger receptor class B member 1 Homo sapiens 111-132 17166497-7 2007 Furthermore, 100 nM of R-154716 completely inhibited [(3)H]COE-labeled HDL uptake induced by overexpression of scavenger receptor BI (SR-BI) in HEK293 cells. Tritium 54-58 scavenger receptor class B member 1 Homo sapiens 134-139 18539593-5 2008 The expressed sigma-1R, N-fragment, and C-fragment exhibited normal, low affinity, and no [3H](+)-pentazocine binding activity, respectively. Tritium 91-94 sigma non-opioid intracellular receptor 1 Homo sapiens 14-22 18599737-5 2008 Mice overexpressing CEH had significantly higher [3H]cholesterol radiolabel in bile and feces, and it was associated with bile acids. Tritium 50-52 epoxide hydrolase 2, cytoplasmic Mus musculus 20-23 12243343-6 2002 A conventional [3H]-thymidine incorporation study showed that the growth rate of the Cx26-transfected cells was significantly decreased (70%), compared to that of the control BICR-M1Rk. Tritium 16-18 gap junction protein, beta 2 Rattus norvegicus 85-89 18625316-5 2008 We evaluated six related steroidal compounds 8a-8f differing in the nature of the 17alpha ester side chain for the inhibition of [3H] R5020 binding to the PR. Tritium 130-132 progesterone receptor Mus musculus 155-157 18625316-6 2008 The IC50 values for the displacement of [3H] R5020 binding to the PR and its relative binding affinities (RBAs) were determined. Tritium 41-43 progesterone receptor Mus musculus 66-68 17600081-2 2007 The new assay, which does not require high-performance liquid chromatography (HPLC) separation or mass spectrometric detection, is based on the release of tritium as tritiated water that occurs upon CYP2C19-mediated 4"-hydroxylation of (S)-mephenytoin labeled with tritium in the 4" position. Tritium 155-162 cytochrome P450 family 2 subfamily C member 19 Homo sapiens 199-206 17600081-2 2007 The new assay, which does not require high-performance liquid chromatography (HPLC) separation or mass spectrometric detection, is based on the release of tritium as tritiated water that occurs upon CYP2C19-mediated 4"-hydroxylation of (S)-mephenytoin labeled with tritium in the 4" position. Tritium 265-272 cytochrome P450 family 2 subfamily C member 19 Homo sapiens 199-206 12039969-3 2002 Membranes isolated from [(3)H]arachidonic acid-labeled Sf9 cells expressing cPLA(2)gamma, constitutively release [(3)H]arachidonic acid. Tritium 25-29 phospholipase A2 group IVC Homo sapiens 76-88 17573188-10 2007 CONCLUSIONS: Intravenous tPA within 3h was safe and feasible, and possibly effective in clinical practice. Tritium 36-38 chromosome 20 open reading frame 181 Homo sapiens 25-28 18554813-5 2008 NMDA-mediated [(3)H]ACh release was completely prevented by MK-801, 5,7-diclorokynurenic acid and ifenprodil, while it was strongly inhibited by a lower external pH, suggesting that the involved NMDA receptors contain NR1 and NR2B subunits. Tritium 15-19 glutamate ionotropic receptor NMDA type subunit 2B Rattus norvegicus 226-230 18393501-4 2008 Collision-induced dissociation of the [OT - 3H + Cu](-) complex yielded exclusively C-terminal Cu(2+)-containing fragments (Cu(2+)fragment(3-)), suggesting that the Cu(2+) ligation site includes deprotonated C-terminal backbone amide nitrogen atoms and the N-terminal amino nitrogen atom in [OT - 3H + Cu](-). Tritium 44-46 oxytocin/neurophysin I prepropeptide Homo sapiens 292-294 18393501-4 2008 Collision-induced dissociation of the [OT - 3H + Cu](-) complex yielded exclusively C-terminal Cu(2+)-containing fragments (Cu(2+)fragment(3-)), suggesting that the Cu(2+) ligation site includes deprotonated C-terminal backbone amide nitrogen atoms and the N-terminal amino nitrogen atom in [OT - 3H + Cu](-). Tritium 297-299 oxytocin/neurophysin I prepropeptide Homo sapiens 39-41 17686476-0 2007 3H-serotonin as a marker of oscillatory insulin secretion in clonal beta-cells (INS-1). Tritium 0-2 forkhead box M1 Homo sapiens 80-85 12150980-5 2002 TRAIL alone induced 25% cell death after a 3-h treatment, but in cells pretreated with EGF-R inhibitors, TRAIL induced cell death to more than 70% after 3h treatment. Tritium 153-155 epidermal growth factor Homo sapiens 87-90 18423037-8 2008 beta2AR expression was confirmed by performing ligand binding assays using the beta-adrenergic antagonist [3H] dihydroalprenolol ([3H]DHA). Tritium 106-109 adrenoceptor beta 2 Homo sapiens 0-7 17586510-7 2008 A single 8 mg/kg infusion of lipid-free apoA-I administered 3h post-collar insertion reduced neutrophil recruitment into the vessel wall, and MPO expression, as well as endothelial expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) by >85% (p<0.01 for all). Tritium 60-62 apolipoprotein A-I Oryctolagus cuniculus 40-46 17586510-7 2008 A single 8 mg/kg infusion of lipid-free apoA-I administered 3h post-collar insertion reduced neutrophil recruitment into the vessel wall, and MPO expression, as well as endothelial expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) by >85% (p<0.01 for all). Tritium 60-62 myeloperoxidase Oryctolagus cuniculus 142-145 17570647-0 2008 Specific binding of [3H]Ro 19-6327 (lazabemide) to monoamine oxidase B is increased in frontal cortex of suicide victims after controlling for age at death. Tritium 21-23 monoamine oxidase B Homo sapiens 51-70 17570647-3 2008 We have evaluated the association of MAO-B density (assessed by [3H]Ro 19-6327 - lazabemide - binding) with type of death (suicide victims vs non-suicide controls) after controlling for age at death. Tritium 65-67 monoamine oxidase B Homo sapiens 37-42 17591542-9 2007 As an alternative to studying knockout mice, we examined the claimed delta subunit-dependent/gamma2 subunit-independent ethanol/[3H]Ro 15-4513 binding sites on GABAA receptors. Tritium 129-131 gamma-aminobutyric acid (GABA) A receptor, subunit gamma 1 Mus musculus 160-165 17892128-6 2007 The proliferation and suppressive function of the CD4+ CD25+ T cells coming from tumor-bearing mice was measured by [3H]-thymidines incorporation experiment in vitro, and then effect of CD4+ CD25+ T cells originated from hepatocellular carcinoma-bearing mice on tumor growth was observed in vivo. Tritium 117-119 CD4 antigen Mus musculus 50-53 17892128-6 2007 The proliferation and suppressive function of the CD4+ CD25+ T cells coming from tumor-bearing mice was measured by [3H]-thymidines incorporation experiment in vitro, and then effect of CD4+ CD25+ T cells originated from hepatocellular carcinoma-bearing mice on tumor growth was observed in vivo. Tritium 117-119 interleukin 2 receptor, alpha chain Mus musculus 55-59 17292806-4 2007 One of these, xendorphin B1 ((Xen)PDYN-B sequence 96-111: YGGFIRKPDKYKFLNA), is a hexadecapeptide that displaced [3H]naloxone and the radiolabelled kappa opioid, [3H]dynorphin A (1-17), with nanomolar affinity from rat brain membranes. Tritium 163-165 prodynorphin S homeolog Xenopus laevis 34-40 12069693-4 2002 Testosterone treatment of LNCaP cells also resulted in dose dependent, biphasic increases in RAR alpha and gamma mRNAs; increases paralleled that of 3H-thymidine incorporation and were attenuated by the presence of 100 nM RA. Tritium 149-151 retinoic acid receptor alpha Homo sapiens 93-102 18080902-9 2008 Ligand binding densities for [3H]cytosine for alpha4beta2 type nAchR did not show any significant change, whereas [3H]AFDX 384, a ligand for m2mAChR, was significantly increased in the cortex of offspring (120-155% increase) of imidacloprid-treated mothers. Tritium 30-32 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 63-68 16220332-4 2007 METHODS: We investigated the interaction of [(3)H](+/-, RS)- (+, S)- and (-, R)-MDMA with the human SERT, dopamine (DA) transporter (DAT), and norepinephrine (NE) transporter (NET) in stably transfected human embryo kidney (HEK)-293 cells. Tritium 44-49 solute carrier family 6 member 3 Homo sapiens 133-136 11997255-6 2002 Enzymatic conversion of [3H]KG to Glu by glutamic dehydrogenase (GDH) or of [3H]OA to malate by malic dehydrogenase (MDH) "protects" the label from release by base, showing that base-induced isotope release is from keto acid rather than a result of release during the exchange process. Tritium 25-27 glutamate dehydrogenase 1 Homo sapiens 41-63 17082621-11 2006 Finally, CsA dose-dependently inhibited the uptake of fNle-Leu-Phe-Nle-Tyr-Lys-fluoresceine and [3H]fMLF or [125I]WKYMVm binding to FPR. Tritium 97-99 chorionic somatomammotropin hormone 1 Homo sapiens 9-12 17555959-3 2007 Among the compounds synthesized, cyclohexane derivatives 3g and 3h and cycloheptane derivative 3j had potent anticoagulant activity as well as anti-fXa activity. Tritium 64-66 coagulation factor X Homo sapiens 148-151 11997255-6 2002 Enzymatic conversion of [3H]KG to Glu by glutamic dehydrogenase (GDH) or of [3H]OA to malate by malic dehydrogenase (MDH) "protects" the label from release by base, showing that base-induced isotope release is from keto acid rather than a result of release during the exchange process. Tritium 25-27 glutamate dehydrogenase 1 Homo sapiens 65-68 16728647-14 2006 [3H]Cytisine and 125I-alpha-bungarotoxin binding sites were eliminated by beta2 and alpha7 gene deletion, respectively. Tritium 1-3 hemoglobin, beta adult minor chain Mus musculus 74-90 12021434-4 2002 The cooperative urea-induced unfolding of alpha-LA, as monitored by the extent of 3H-methylene labeling, agrees with that measured by circular dichroism spectroscopy in the far and near ultraviolet regions. Tritium 82-84 lactalbumin alpha Bos taurus 42-50 16728647-15 2006 These studies establish that the lower-affinity sites represent a structurally diverse set of sites that require expression of either alpha7, beta2, or beta4 subunits and extend and confirm previous classifications of the higher-affinity [3H]epibatidine binding sites. Tritium 239-241 hemoglobin, beta adult minor chain Mus musculus 142-147 12014965-2 2002 Heptylene-linked bis-(6-chloro)tacrine (3h) was found up to 3000- and 3-fold more potent in inhibiting rat AChE than tacrine and the unsubstituted bis-tacrine 3b, respectively. Tritium 40-42 acetylcholinesterase Rattus norvegicus 107-111 16806307-7 2006 Moreover, dose-response curves of the agonists showed that mu and CB1 receptors mediating inhibition of [3H]glutamate release display a non-additive interaction, whereas these receptors synergistically interact regarding their inhibitory control of [3H]GABA release. Tritium 105-107 cannabinoid receptor 1 Rattus norvegicus 66-69 16806307-8 2006 Finally, the apparent allosteric interaction between antagonists was also observed regarding the effects of other receptor-selective agonists and antagonists at mu opioid and CB1 cannabinoid receptors (mediating inhibition of NMDA-induced [3H]GABA release) and must therefore be a unique property of the receptors involved. Tritium 240-242 cannabinoid receptor 1 Rattus norvegicus 175-178 16828704-2 2006 When GroEL was incubated for 3h with 15 mM H2O2, it retained its quaternary structure, chaperone and ATPase activities. Tritium 29-31 heat shock protein family D (Hsp60) member 1 Homo sapiens 5-10 12031792-6 2002 To define PKC"s role in myotrophin-induced myocyte growth, we incubated neonatal rat myocytes (normal and stretch) with specific inhibitors and found that myotrophin inhibits [3H]leucine incorporation into myocytes and different hypertrophic gene expression in neonatal myocytes. Tritium 176-178 myotrophin Rattus norvegicus 24-34 16691569-3 2006 Both B3- and B3 possess D(3h) symmetry at the equilibrium configuration of their electronic ground state. Tritium 26-28 immunoglobulin kappa variable 4-1 Homo sapiens 5-15 12031792-6 2002 To define PKC"s role in myotrophin-induced myocyte growth, we incubated neonatal rat myocytes (normal and stretch) with specific inhibitors and found that myotrophin inhibits [3H]leucine incorporation into myocytes and different hypertrophic gene expression in neonatal myocytes. Tritium 176-178 myotrophin Rattus norvegicus 155-165 11966513-2 2002 METHODS: The effect of P-gp inhibitors (verapamil, 0-100 microM; PSC 833, 0-20 microM) on the intracellular accumulation of 3H-cortisol and 3H-ciclosporin was studied in confluent layers of human Caco-2 cells (n=6), a P-gp-dependent absorptive intestinal epithelial cell phenotype, and moderately resistant MDRhigh CEM/VBL 100 T cells (n=6). Tritium 124-126 phosphoglycolate phosphatase Homo sapiens 23-27 16787574-7 2006 More importantly, IP3R inhibitors, 2-aminoethoxydiphenyl borate and Xestospongin C, abolished the IP3BM-induced Ca2+ responses, and significantly suppressed alpha1AR-induced cardiomyocyte hypertrophy assayed by cell size, [3H] leucine incorporation and atrial natriuretic factor gene expression, during sustained (48 h) phenylephrine stimulation. Tritium 223-225 inositol 1,4,5-trisphosphate receptor, type 1 Rattus norvegicus 18-22 16627748-2 2006 The Eisai hyperbilirubinemic rat (EHBR) that lacks Mrp2 (multidrug resistance-associated protein 2) was used to appraise the role of Mrp2 in the excretion of [3H]EN and its metabolite [3H]ENA in single-pass rat liver preparations. Tritium 159-161 ATP binding cassette subfamily C member 2 Rattus norvegicus 133-137 16386712-0 2006 Odor increases [3H]phorbol dibutyrate binding to protein kinase C in olfactory structures of rat brain. Tritium 16-18 protein kinase C, gamma Rattus norvegicus 49-65 11597792-3 2001 One microgram of recombinant matrilysin is confirmed to have increased [3H]-thymidine uptake in human umbilical vein endothelial cells. Tritium 72-74 matrix metallopeptidase 7 Homo sapiens 29-39 16402924-8 2006 Progesterone (Pg), E2 and EGF incubated under the same conditions had a stimulatory effect on [3H]-thymidine incorporation into MCF-7 cells, whereas TGF-beta2 inhibited this parameter. Tritium 95-97 cystatin 12, pseudogene Homo sapiens 19-29 16192276-8 2005 The amount and activity of a key enzyme of the CDP-ethanolamine pathway for PE synthesis, CTP:phosphoethanolamine cytidylyltransferase, were increased by 35-40 and 100%, respectively, in tissues of Pisd+/- mice, as judged by immunoblotting; PE synthesis from [3H]ethanolamine was correspondingly increased in hepatocytes. Tritium 260-262 phosphate cytidylyltransferase 2, ethanolamine Mus musculus 90-134 11714843-12 2001 LPL stimulated [3H]cholesteryl oleyl ether uptake from labeled HDL3 by HEK 293 cells substantially, showing that LPL can induce selective CE uptake from HDL3 independent of SR-BI. Tritium 16-18 lipoprotein lipase Homo sapiens 0-3 11714843-12 2001 LPL stimulated [3H]cholesteryl oleyl ether uptake from labeled HDL3 by HEK 293 cells substantially, showing that LPL can induce selective CE uptake from HDL3 independent of SR-BI. Tritium 16-18 lipoprotein lipase Homo sapiens 113-116 16024575-6 2005 At these concentrations, ANG-(1-7) had inhibitory effects on collagen synthesis as assessed by [3H]proline incorporation and decreased mRNA expression of growth factors in ARCFs. Tritium 96-98 angiogenin Rattus norvegicus 25-33 11597569-3 2001 Short-term (30 min) agonist challenge of recombinant EP4 expressed in human embryonic kidney 293 cells (EP4-HEK293 cells) with PGE2 (1 microM) resulted in the desensitization of intracellular cyclic AMP (cAMP) accumulation and a reduction in cell surface [3H]PGE2 specific binding sites. Tritium 256-258 prostaglandin E receptor 4 Homo sapiens 53-56 16024575-9 2005 ANG-(1-7) pretreatment also inhibited the stimulatory effects of conditioned medium from ANG II-treated ARCFs on [3H]leucine incorporation and atrial natriuretic factor mRNA expression, markers of hypertrophy, in cardiomyocytes. Tritium 114-116 angiogenin Rattus norvegicus 0-8 16024575-9 2005 ANG-(1-7) pretreatment also inhibited the stimulatory effects of conditioned medium from ANG II-treated ARCFs on [3H]leucine incorporation and atrial natriuretic factor mRNA expression, markers of hypertrophy, in cardiomyocytes. Tritium 114-116 angiogenin Rattus norvegicus 0-3 16251407-5 2005 Interferon (IFN)-gamma, which signals growth arrest via Stat1, inhibited EGF- or PDGF-stimulated mitogenesis in wild-type fibroblasts but enhanced [3H]thymidine uptake in Stat1-/- fibroblasts. Tritium 148-150 signal transducer and activator of transcription 1 Mus musculus 171-176 16251407-6 2005 Moreover, IFN-gamma treatment in the absence of growth factors induced a concentration-dependent increase in [3H]thymidine uptake in Stat1-/- but not wild-type fibroblasts. Tritium 110-112 signal transducer and activator of transcription 1 Mus musculus 133-138 11597569-3 2001 Short-term (30 min) agonist challenge of recombinant EP4 expressed in human embryonic kidney 293 cells (EP4-HEK293 cells) with PGE2 (1 microM) resulted in the desensitization of intracellular cyclic AMP (cAMP) accumulation and a reduction in cell surface [3H]PGE2 specific binding sites. Tritium 256-258 prostaglandin E receptor 4 Homo sapiens 104-107 11557259-9 2001 The binding of [(3)H]2-PMPA also showed tissue specificity in that tissues previously reported to be devoid of GCPII enzymatic activity were devoid of [(3)H]2-PMPA binding. Tritium 15-21 folate hydrolase 1 Rattus norvegicus 111-116 16170326-9 2005 In conclusion, the present study show that the allosteric modulators PS or 3alpha5betaS change [3H]ifenprodil binding kinetics in a way indicating conformational alteration of its binding site on the NR2B subunit. Tritium 96-98 glutamate ionotropic receptor NMDA type subunit 2B Rattus norvegicus 200-204 11389172-1 2001 [(3)H](2S,4R)-4-Methylglutamate ([(3)H]4MG), used previously as a ligand for low-affinity kainate receptors, was employed to establish a binding assay for glutamate transporters (GluTs), as 4MG has also been shown to have affinity for the glial GluTs, GLT1 and GLAST. Tritium 0-5 solute carrier family 1 member 3 Rattus norvegicus 261-266 16157790-3 2005 Here we tested the hypothesis that upregulation of c-Src by aldosterone plays a role in increased mitogen-activated protein (MAP) kinase activation, [3H]-proline incorporation, and NADPH-driven generation of reactive oxygen species, thereby inducing cell growth, collagen production, and inflammation, respectively, in vascular smooth muscle cells from spontaneously hypertensive rats. Tritium 150-152 SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus 51-56 16061219-5 2005 In brain and liver, competition curves for beta-carbolines against [3H]Ro41-1049 (MAO-A) and [3H]Ro19-6327 (MAO-B) were monophasic and resulted in different drug potencies for the two MAO isozymes (higher affinities for MAO-A) and in similar pharmacological profiles in both tissues. Tritium 94-96 monoamine oxidase B Rattus norvegicus 108-113 11376598-9 2001 In contrast, the antioxidant catalase (1000 U/ml) significantly attenuated methylmercury-induced inhibition of 3H-aspartate uptake, suggesting that excess reactive oxygen species, specifically H2O2, inhibit the function of an astrocytic excitatory amino acid transporter (EAAT1). Tritium 111-113 solute carrier family 1 member 3 Rattus norvegicus 272-277 15967578-7 2005 In parallel experiments, radioactive surface measurements permitted measuring binding of tritium-labeled factor Xa onto a PS monolayer and calculate an association constant, 6x10(6) M(-1). Tritium 89-96 coagulation factor X Homo sapiens 105-114 11306243-7 2001 In addition, thrombin-induced [3H]-thymidine incorporation and p42/p44 MAPK phosphorylation was completely inhibited by PD98059 (an inhibitor of MEK1/2), indicating that activation of MEK1/2 was required for these responses. Tritium 31-33 mitogen-activated protein kinase kinase 1 Homo sapiens 145-151 11321363-4 2001 Whereas IL-1alpha and TNFalpha significantly increased the levels of IL-8, C5a decreased the IL-8 production after 48 h. In addition, the ability of IL-1alpha, TNFalpha, C5a, fMLP and IL-8 to induce cell proliferation was compared by means of a 3H-thymidine incorporation assay. Tritium 245-247 interleukin 1 alpha Homo sapiens 149-158 16180991-4 2005 Microsomes containing 11betaHSD1 were incubated in the presence of NADPH and [3H]cortisone, and the product, [3H]cortisol, was specifically detected in the mixture by a monoclonal antibody coupled to protein A-coated SPA beads with greater than 2 log higher affinity for cortisol than cortisone. Tritium 110-112 2,4-dienoyl-CoA reductase 1 Homo sapiens 67-72 17658679-5 2007 Prolonged insulin treatment (greater than 3h) significantly reduced GHR mRNA, and removal of insulin led to a gradual recovery. Tritium 42-44 growth hormone receptor Rattus norvegicus 68-71 11330337-0 2001 Differential inhibition of [3H]-oxotremorine-M and [3H]-quinuclinidyl benzilate binding to muscarinic receptors in rat brain membranes with acetylcholinesterase inhibitors. Tritium 52-54 acetylcholinesterase Rattus norvegicus 140-160 17623082-6 2007 Since all negative strand RNA viruses are required to have the ribonucleoprotein complex as their active genomic templates, it is perceivable that the (5H+3H) structure is a common motif in the nucleoprotein of negative strand RNA viruses. Tritium 155-157 nucleoprotein Variegated squirrel bornavirus 1 67-80 17379646-8 2007 Subcellular fractionation studies further support the plasma membrane as a site of GPR30 action with specific [3H]17beta-E2 binding and G protein activation associated with plasma membrane but not microsomal, or other fractions, prepared from HEK-293 or SKBR3 breast cancer cells. Tritium 111-113 G protein-coupled estrogen receptor 1 Homo sapiens 83-88 15946645-3 2005 IFN-gamma decreased ABCA1 mRNA 1h following IFN-gamma addition and was maximally reduced by 3h. Tritium 92-94 ATP-binding cassette, sub-family A (ABC1), member 1 Mus musculus 20-25 15925360-4 2005 The MEN13510 interaction with the human NK2 receptor was evaluated by means of heterologous inhibition binding experiments, by using agonist and antagonist radioligands ([125I]NKA, [3H]nepadutant, [3H]saredutant) at a series of mutant receptors having single aminoacidic substitutions of residues located in transmembrane (TM) segments 3, 4, 5, 6, and 7. Tritium 198-200 tachykinin receptor 2 Homo sapiens 40-52 15840715-4 2005 CPO-BP is a 50-kDa membrane-bound serine hydrolase measured by derivatization with [(3)H]CPO and SDS/PAGE or filtration binding assay. Tritium 85-88 neutral cholesterol ester hydrolase 1 Mus musculus 0-6 11330337-2 2001 In the present study, the capacity of acetylcholinesterase inhibitors to interact with muscarinic receptors was assessed by their ability to displace both [3H]-oxotremorine-M and [3H]-quinuclinidyl benzilate binding in rat brain membranes. Tritium 156-158 acetylcholinesterase Rattus norvegicus 38-58 17395707-5 2007 There was a gradient of response to IGF-I, with the greatest proliferative response in high-density cells corresponding to the reserve zone, as measured by [3H]thymidine uptake. Tritium 157-159 IGFI Bos taurus 36-41 11330337-2 2001 In the present study, the capacity of acetylcholinesterase inhibitors to interact with muscarinic receptors was assessed by their ability to displace both [3H]-oxotremorine-M and [3H]-quinuclinidyl benzilate binding in rat brain membranes. Tritium 180-182 acetylcholinesterase Rattus norvegicus 38-58 15664626-5 2005 In contrast to the results obtained by transfection, exposure of Balb/c 3T3 fibroblasts to exogenously added T-KG leads to a dose-dependent increase in [3H]-thymidine incorporation. Tritium 153-155 kininogen 2 Rattus norvegicus 109-113 11330337-4 2001 A series of compounds, representative of the principal classes of acetylcholinesterase inhibitors, displaced [3H]-oxotremorine-M binding with high-to-moderate potency (ambenonium>neostigmine=pyridostigmine=tacrine>physostigmine> edrophonium=galanthamine>desoxypeganine) whereas only ambenonium and tacrine displaced [3H]-quinuclinidyl benzilate binding. Tritium 110-112 acetylcholinesterase Rattus norvegicus 66-86 15358595-6 2005 Inhibition of phosphatidylinositol (PI)3-kinase with LY-294002 or mTOR/p70S6K1 with rapamycin reduced [3H]thymidine incorporation by 50%, i.e., to levels comparable to those achieved by addition of either AG-1478 or GM-6001. Tritium 103-105 mechanistic target of rapamycin kinase Rattus norvegicus 66-70 17219421-3 2007 Overexpression of PKCdelta in NMuMG cells leads to a significant increase in [3H]-tymidine incorporation and cell proliferation, as well as enhanced extracellular signal-regulated kinase (ERK)-mitogen-activated protein kinase (MAPK) activation. Tritium 78-80 protein kinase C, delta Mus musculus 18-26 11330337-7 2001 The capacity of acetylcholinesterase inhibitors, with the exception of tacrine and ambenonium, to displace bound [3H]-oxotremorine-M in preference to [3H]quinuclinidyl benzilate predicts that the former compounds could act as potential agonists at muscarinic receptors. Tritium 114-116 acetylcholinesterase Rattus norvegicus 16-36 11237060-7 2001 Inversely, response to GM-CSF, as defined by an increase in 3H-TdR incorporation >1.5- fold over control values after 48h of GM-CSF exposure, was significantly lower for patients with a favorable karyotype (group II) as compared to group I (P = 0.04) and group III (P = 0.013). Tritium 60-62 colony stimulating factor 2 Homo sapiens 23-29 17078924-5 2007 The truncated VDR weakly bound [3H]-1,25(OH)2D3 but was able to heterodimerize with RXR, bind to DNA and interact with the corepressor hairless (HR). Tritium 32-34 vitamin D receptor Homo sapiens 14-17 17234899-8 2007 DAT cells in Krebs-HEPES buffer had an apparent spontaneous [3H]dopamine loss, but it could not be blocked by either methylphenidate or a PKC inhibitor. Tritium 61-63 solute carrier family 6 member 3 Macaca mulatta 0-3 17292806-4 2007 One of these, xendorphin B1 ((Xen)PDYN-B sequence 96-111: YGGFIRKPDKYKFLNA), is a hexadecapeptide that displaced [3H]naloxone and the radiolabelled kappa opioid, [3H]dynorphin A (1-17), with nanomolar affinity from rat brain membranes. Tritium 114-116 prodynorphin S homeolog Xenopus laevis 34-40 16165286-4 2005 That [3H]-acetylcholine was synthesized from [3H]-choline was demonstrated by the lack of [3H]-acetylcholine release following incubation with either the choline uptake inhibitor hemicholinium or the choline acetyltransferase inhibitor bromoacetylcholine. Tritium 6-8 choline O-acetyltransferase Rattus norvegicus 200-225 16165286-4 2005 That [3H]-acetylcholine was synthesized from [3H]-choline was demonstrated by the lack of [3H]-acetylcholine release following incubation with either the choline uptake inhibitor hemicholinium or the choline acetyltransferase inhibitor bromoacetylcholine. Tritium 46-48 choline O-acetyltransferase Rattus norvegicus 200-225 11157497-7 2001 Inhibition of plasminogen binding to annexin II on RAW264.7 macrophages significantly impaired their ability to activate plasminogen and degrade [3H]-glucosamine-labeled extracellular matrices. Tritium 146-148 annexin A2 Homo sapiens 37-47 11157849-8 2001 CD28-deficient and CD7/CD28-deficient thymocytes had decreased [3H]thymidine incorporation responses to concanavalin A (Con A) stimulation compared to control mice (P < or = 0.01 and P < or = 0.05 respectively). Tritium 64-66 CD28 antigen Mus musculus 0-4 15452358-6 2005 A receptor binding assay using rat forebrain and spinal cord membrane preparations demonstrated that [3H]CP-101,606 bound to the brain NR2B receptor with a greater extent compared to the spinal cord one. Tritium 102-104 glutamate ionotropic receptor NMDA type subunit 2B Rattus norvegicus 135-139 18176897-2 2007 Our data indicated that ASN (10 microM) inhibited [3H]AA incorporation into phosphatidylethanolamine (PE), phosphatidylcholine (PC) and phosphatidylserine (PS) together with phosphatidic acid (PA) by 13%, 27% and 38%, respectively. Tritium 51-53 synuclein alpha Rattus norvegicus 24-27 18176897-4 2007 Because ASN and NAC lower the level of AA-CoA we suggest that inhibition of AA-CoA synthase is responsible for disturbed [3H]AA incorporation. Tritium 122-124 synuclein alpha Rattus norvegicus 8-11 15302892-3 2004 The present studies determined the relative impact of Mrp2 and P-gp on the hepatobiliary disposition of [3H]DPDPE in isolated perfused rat livers (IPLs). Tritium 105-107 ATP binding cassette subfamily C member 2 Rattus norvegicus 54-58 11157849-8 2001 CD28-deficient and CD7/CD28-deficient thymocytes had decreased [3H]thymidine incorporation responses to concanavalin A (Con A) stimulation compared to control mice (P < or = 0.01 and P < or = 0.05 respectively). Tritium 64-66 CD28 antigen Mus musculus 23-27 15302892-12 2004 Mrp2 is the primary mechanism for [3H]DPDPE biliary excretion, and P-gp facilitates excretion of [3H]DPDPE only in the absence of functional Mrp2. Tritium 35-37 ATP binding cassette subfamily C member 2 Rattus norvegicus 0-4 15472122-7 2004 Also, less radioactivity was recovered in the liver of ARH-/- or LDLR-/- mice when [3H]cholesteryl oleyl ether (CE)-labeled LDL was injected. Tritium 84-86 low density lipoprotein receptor Mus musculus 65-69 15280218-3 2004 The full coding region of rat Mrp2 was cloned into the baculovirus genome, the recombinant baculovirus used to infect Sf9 cells, and ATP-dependent transport of 3H-E23G and 3H-E217G in Sf9 cell membranes was characterized. Tritium 160-162 ATP binding cassette subfamily C member 2 Rattus norvegicus 30-34 17034769-8 2006 Neonatal astrocytes from PEPT2-/- mice had a 62% reduction in [14C]ALA uptake and a 92% reduction in [3H]carnosine uptake compared to PEPT2+/+ mice. Tritium 102-104 solute carrier family 15 (H+/peptide transporter), member 2 Mus musculus 25-30 16480775-2 2006 VMAT-2 activity has traditionally been measured in small synaptic vesicles isolated from rat striatum by monitoring [3H] DA uptake and in cellular expression systems using fast scan cyclic voltammetry. Tritium 117-119 solute carrier family 18 member A2 Rattus norvegicus 0-6 15280218-3 2004 The full coding region of rat Mrp2 was cloned into the baculovirus genome, the recombinant baculovirus used to infect Sf9 cells, and ATP-dependent transport of 3H-E23G and 3H-E217G in Sf9 cell membranes was characterized. Tritium 172-174 ATP binding cassette subfamily C member 2 Rattus norvegicus 30-34 11405287-7 2001 This difference in [3H]-taxol flux could be totally abolished by inclusion of (+/-)-verapamil (0.2 mM), a known inhibitor of P-gp, in the incubation medium. Tritium 20-22 phosphoglycolate phosphatase Homo sapiens 125-129 15339391-6 2004 Parthenolide, an inhibitor of STAT, suppressed CT-1-induced [3H]leucine incorporation by 88.3 % and protein-to-DNA ratio by 75.0 %. Tritium 61-63 cardiotrophin 1 Rattus norvegicus 47-51 15339391-7 2004 U0126, an MEK1/2 inhibitor, increased CT-1-induced the phosphorylation of STAT3 in a dose-dependent manner and, consistently, augmented CT-1-induced increase in [3H]leucine incorporation and cellular protein-to-DNA ratio by 17.6 % and 16.3 %, respectively. Tritium 162-164 cardiotrophin 1 Rattus norvegicus 38-42 16886676-3 2006 MATERIALS AND METHODS: Using primary human keratinocytes, CYP24A1 expression was examined at the mRNA level by dot-blot and Northern blot hybridization, and at the enzyme activity level by analysing HPLC profiles from incubations with 3H-labelled VD metabolites. Tritium 235-237 cytochrome P450 family 24 subfamily A member 1 Homo sapiens 58-65 16617163-4 2006 In HeLa cells with recombinant human concentrative nucleoside transporter (hCNT) 1 or hCNT3 and pharmacologically blocked hENT1 and hENT2, transport of 10 microM[3H]TaraC and [3H]araC was not detected. Tritium 162-164 solute carrier family 28 member 1 Homo sapiens 37-82 15226216-4 2004 Ang II-induced myocyte hypertrophy assessed by increments in cell size, [3H]leucine uptake, and protein content was suppressed by HO-1 overexpression. Tritium 73-75 heme oxygenase 1 Rattus norvegicus 130-134 16617163-4 2006 In HeLa cells with recombinant human concentrative nucleoside transporter (hCNT) 1 or hCNT3 and pharmacologically blocked hENT1 and hENT2, transport of 10 microM[3H]TaraC and [3H]araC was not detected. Tritium 176-178 solute carrier family 28 member 1 Homo sapiens 37-82 11157142-2 2001 METHODS: Granzyme B was added to [(3)H]proline/[(35)S]sulphate-labelled cartilage matrices and to cartilage explants. Tritium 35-38 granzyme B Homo sapiens 9-19 15016836-4 2004 The pharmacological profiles of the agonist binding sites of these putative nAChR subtypes were examined in competition studies in which unlabeled nicotinic ligands, including 10 agonists and two antagonists, competed against [3H]EB. Tritium 227-230 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 76-81 11216647-6 2000 A significant increase in [3H]thymidine incorporation by cells after a similar short-term exposure to IGF-1 suggests that the measured increase in extracellular acidification following IGF-1 addition is physiologically relevant. Tritium 27-29 insulin like growth factor 1 Bos taurus 102-107 15473137-6 2004 Insulin (10 nM) increased [3H]2-deoxyglucose (2-DOG) uptake to 200% from basal level (100%) in cultured adipocytes transfected with a vector alone. Tritium 27-29 insulin Canis lupus familiaris 0-7 16712814-5 2006 In an extension of our previous work, the current study, using receptor autoradiography, compared binding (by [3H]WIN35428) of the dopamine transporter (DAT) in animals having experienced either brief or extended daily access to cocaine over 8 days, followed by 14 days of withdrawal. Tritium 111-113 solute carrier family 6 member 3 Homo sapiens 131-151 16712814-5 2006 In an extension of our previous work, the current study, using receptor autoradiography, compared binding (by [3H]WIN35428) of the dopamine transporter (DAT) in animals having experienced either brief or extended daily access to cocaine over 8 days, followed by 14 days of withdrawal. Tritium 111-113 solute carrier family 6 member 3 Homo sapiens 153-156 11216647-6 2000 A significant increase in [3H]thymidine incorporation by cells after a similar short-term exposure to IGF-1 suggests that the measured increase in extracellular acidification following IGF-1 addition is physiologically relevant. Tritium 27-29 insulin like growth factor 1 Bos taurus 185-190 16537968-5 2006 In addition, apoC-I increased the postprandial TG response to an intragastric olive oil load (+120%; P < 0.05) and decreased the uptake of [3H]TG-derived FFAs from intravenously administered VLDL-like emulsion particles by gonadal and perirenal white adipose tissue (WAT) (-34% and -25%, respectively; P < 0.05). Tritium 143-145 apolipoprotein C-I Mus musculus 13-19 15090047-5 2004 Our binding studies using [3H]-(+)SKF-10,047 indicated that amantadine and memantine behave as ligands of the sigma(1) receptor in rat forebrain homogenates (Ki values of 7.44 +/- 0.82 and 2.60 +/- 0.62 microm, respectively). Tritium 27-29 sigma non-opioid intracellular receptor 1 Rattus norvegicus 110-127 11207432-18 2000 Moreover, antisense treatments against the purported sigma(1) receptor gene reduced specific sigma(1)/[3H](+)pentazocine binding and modulated cognitive behaviors associated with NMDA receptor blockade providing further evidence for the functional relevance of the cloned gene. Tritium 103-105 sigma non-opioid intracellular receptor 1 Mus musculus 53-70 16647690-5 2006 There was a significant decrease in binding of the specific GABABR antagonist, [3H]CGP-54626A at postnatal day (PN)7 and PN14 in SSADH(-/-) when compared to wild type control animals (SSADH(+/+)), particularly in hippocampus. Tritium 80-82 aldehyde dehydrogenase 5 family member A1 Homo sapiens 129-139 16647690-5 2006 There was a significant decrease in binding of the specific GABABR antagonist, [3H]CGP-54626A at postnatal day (PN)7 and PN14 in SSADH(-/-) when compared to wild type control animals (SSADH(+/+)), particularly in hippocampus. Tritium 80-82 aldehyde dehydrogenase 5 family member A1 Homo sapiens 129-134 15018919-5 2004 The affinity and selectivity of the novel compounds to VAChT sites were analyzed by competitive radioligand binding studies in rat brain and liver membrane preparations using tritium labeled radioligands. Tritium 175-182 solute carrier family 18 member A3 Rattus norvegicus 55-60 11080213-6 2000 Using 10 nM: [(3)H]quisqualate, a high density of binding was detected in various brain regions with the following rank order of increasing levels: medulla, thalamus, olfactory bulb, cerebral cortex, spinal cord dorsal horn, olfactory tubercle, dentate gyrus molecular layer, CA1-3 oriens layer of hippocampus, striatum, and cerebellar molecular layer. Tritium 13-18 carbonic anhydrase 1 Rattus norvegicus 276-279 15016089-3 2004 The novel agonist [Arg(14),Lys(15)]N/OFQ also inhibited [(3)H]-5-HT overflow, but the concentration-response curve was biphasic and the efficacy higher ( approximately -45%). Tritium 57-61 prepronociceptin Mus musculus 35-40 14741716-4 2004 Binding of [3H]CCK-8 was observed in WT and K130V, but not in K120V and R352V. Tritium 12-14 cholecystokinin Cricetulus griseus 15-18 16213169-3 2006 RESULTS: The CAD prevented the plasma membrane expression of hM1 receptors by causing the formation and intracellular retention of CAD-fused receptor aggregates as determined using intact cell [3H]NMS binding assays and epi-fluorescence microscopy, respectively. Tritium 194-196 cholinergic receptor muscarinic 1 Homo sapiens 61-64 16434140-5 2006 After immunoprecipitation of SV2A from solubilized rat brain membranes, binding properties of [3H]ucb 30889 to SV2A and association with synaptotagmin I were maintained. Tritium 95-97 synaptic vesicle glycoprotein 2a Rattus norvegicus 29-33 16434140-5 2006 After immunoprecipitation of SV2A from solubilized rat brain membranes, binding properties of [3H]ucb 30889 to SV2A and association with synaptotagmin I were maintained. Tritium 95-97 synaptic vesicle glycoprotein 2a Rattus norvegicus 111-115 15497468-5 2004 Multiple linear regression revealed MTHFR 677 TT (p=0.01), folate (p=0.04) and vitamin B12 (p=0.06) as determinants of fasting homocysteine concentration; only MTHFR 677TT was related to 3-h (p=0.04) and 6-h (p=0.004) post-load homocysteine concentration. Tritium 187-190 methylenetetrahydrofolate reductase Homo sapiens 36-41 11152384-11 2000 In vitro, 0.1-1 mM concentrations of BAL inhibited [3H]glutamate and [3H]MK-801 binding, but increased the binding of [3H]muscimol to brain synaptic plasma membrane. Tritium 52-54 poly (ADP-ribose) polymerase family, member 9 Mus musculus 37-40 14625043-3 2003 In patients with Ammon"s horn sclerosis, significant decreases in [3H]CG54626A binding were observed in subfields CA1 and CA3 of the hippocampus proper and the dentate hilus. Tritium 67-69 carbonic anhydrase 1 Homo sapiens 114-117 14568345-5 2003 NIC-induced [3H]-NE and [3H]-DA release responses were both calcium-dependent and attenuated by the sodium channel antagonist, tetrodotoxin (TTX) and by the nAChR antagonists mecamylamine (MEC) and dihydro-beta-erythroidine (DHbetaE), but not by D-tubocurare (D-TC). Tritium 13-15 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 157-162 14568345-5 2003 NIC-induced [3H]-NE and [3H]-DA release responses were both calcium-dependent and attenuated by the sodium channel antagonist, tetrodotoxin (TTX) and by the nAChR antagonists mecamylamine (MEC) and dihydro-beta-erythroidine (DHbetaE), but not by D-tubocurare (D-TC). Tritium 25-27 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 157-162 14568345-6 2003 The modulation of [3H]-5-HT release by nAChR agonists was distinct from that seen for catecholamines. Tritium 19-21 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 39-44 16556440-10 2006 The results showed that the binding properties of levetiracetam and [3H]ucb 30889, an analogue that was previously characterized as a suitable ligand for levetiracetam binding site/SV2A in rat brain [Gillard, M., Fuks, B., Michel, P., Vertongen, P., Massingham, R. Chatelain, P., 2003. Tritium 69-71 synaptic vesicle glycoprotein 2a Rattus norvegicus 181-185 16556440-17 2006 [3H]ucb 30889 binding in human brain and to SV2A was saturable and reversible. Tritium 1-3 synaptic vesicle glycoprotein 2A Homo sapiens 44-48 16556440-26 2006 It cannot be explained by putative binding of [3H]ucb 30889 to SV2B or C isoforms but may reflect different patterns of SV2A glycosylation or the formation of SV2A oligomers. Tritium 47-49 synaptic vesicle glycoprotein 2B Homo sapiens 63-67 16603631-5 2006 Further, TRAM, but not TRAM-G2A, was radiolabeled with [3H]myristate in vivo. Tritium 56-58 toll-like receptor adaptor molecule 2 Mus musculus 9-13 16603631-5 2006 Further, TRAM, but not TRAM-G2A, was radiolabeled with [3H]myristate in vivo. Tritium 56-58 toll-like receptor adaptor molecule 2 Mus musculus 9-12 11152384-11 2000 In vitro, 0.1-1 mM concentrations of BAL inhibited [3H]glutamate and [3H]MK-801 binding, but increased the binding of [3H]muscimol to brain synaptic plasma membrane. Tritium 70-72 poly (ADP-ribose) polymerase family, member 9 Mus musculus 37-40 16529737-2 2006 Bradykinin did not induce mitogenic responses by itself, but concentration-dependently augmented growth factor-induced [3H]thymidine incorporation and cell proliferation. Tritium 120-122 kininogen 1 Bos taurus 0-10 12906922-8 2003 TNF-alpha increased [3H]vinblastine accumulation indicating a time and concentration-dependent decrease of P-glycoprotein activity. Tritium 21-23 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 107-121 11152384-11 2000 In vitro, 0.1-1 mM concentrations of BAL inhibited [3H]glutamate and [3H]MK-801 binding, but increased the binding of [3H]muscimol to brain synaptic plasma membrane. Tritium 70-72 poly (ADP-ribose) polymerase family, member 9 Mus musculus 37-40 11095538-3 2000 In addition OMP promotes an increase in uptake of [3H]TdR in liver, but TGF-alpha has no effect. Tritium 51-53 olfactory marker protein Rattus norvegicus 12-15 12888804-7 2003 ORL1 receptor knockout mice exhibited a hyperfunction of N-methyl-D-aspartate (NMDA) receptor, as evidenced by an increase in [3H]MK-801 binding, NMDA-evoked 45Ca2+ uptake and activation of Ca2+/calmodulin-dependent protein kinase II (CaMKII) activity and its phosphorylation as compared with those in wild-type mice. Tritium 127-129 opioid receptor-like 1 Mus musculus 0-4 12942748-2 2003 Enkephalinase activity was assayed in vitro by the rate of 3H-Leu-enkephalin hydrolysis in the presence of the tested peptides. Tritium 59-61 membrane metallo-endopeptidase Rattus norvegicus 0-13 16256076-12 2006 One potential limitation of [3H]FW is a large preference for subunits GluR1 and GluR2 (KD 4-10 nM) over GluR3 and GluR4 (160-600 nM) which implies that tests with brain membranes preferentially reveal drug effects produced at the former two subunits. Tritium 29-31 glutamate ionotropic receptor AMPA type subunit 4 Rattus norvegicus 114-119 11075960-7 2000 Treatment of EGFR-TI7 cells with C2-cer (0.1-10 microM) did not affect cell viability, but prevented EGF-induced 3H-thymidine incorporation in a dose-dependent manner. Tritium 113-115 epidermal growth factor receptor Mus musculus 13-17 16599912-5 2006 No research existed for several clades of invertebrates; we therefore tested for cannabinoid receptors in seven representative species, using tritiated ligand binding assays with [3H]CP55,940 displaced by the CB1-selective antagonist SR141716A. Tritium 180-182 cannabinoid receptor 1 Rattus norvegicus 209-212 12753083-3 2003 The predominant nAChR labeled by agonists in the cerebral cortex is an alpha 4 beta 2 subtype, whereas the predominant nicotinic receptors in the adrenal gland, superior cervical ganglia and pineal gland contain an alpha 3 subunit, and they do not bind either [3H](-)nicotine or [3H]cytisine with high affinity. Tritium 261-263 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 16-21 12753083-3 2003 The predominant nAChR labeled by agonists in the cerebral cortex is an alpha 4 beta 2 subtype, whereas the predominant nicotinic receptors in the adrenal gland, superior cervical ganglia and pineal gland contain an alpha 3 subunit, and they do not bind either [3H](-)nicotine or [3H]cytisine with high affinity. Tritium 280-282 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 16-21 12670875-4 2003 Induction of endostatin in double transgenic mice with doxycycline-induced expression of VEGF in the retina resulted in significant suppression of leakage of intravascular [3H]mannitol into the retina. Tritium 173-175 collagen, type XVIII, alpha 1 Mus musculus 13-23 12670875-4 2003 Induction of endostatin in double transgenic mice with doxycycline-induced expression of VEGF in the retina resulted in significant suppression of leakage of intravascular [3H]mannitol into the retina. Tritium 173-175 vascular endothelial growth factor A Mus musculus 89-93 12751633-5 2003 Affinities to P-gp were evaluated using a radioligand binding assay based on P-gp-overexpressing cells and [3H]-talinolol as radioligand. Tritium 108-110 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 14-18 16571921-4 2006 [3H]-Thymidine and BrdU incorporation also indicated that both hsp25 and inducible hsp70 were involved in G1 arrest after 1 cGy irradiation. Tritium 1-3 heat shock protein family A (Hsp70) member 4 Homo sapiens 83-88 16702620-4 2006 The 5-HT1B/1D receptor agonist 5-carboxamidotryptamine inhibited 3H overflow only in the presence of atropine. Tritium 65-67 5-hydroxytryptamine receptor 1B Oryctolagus cuniculus 4-10 16702620-5 2006 The 5-HT4 and 5-HT1B/1D receptor agonists 5-HT and 5-methoxytryptamine reduced 3H overflow in the absence and presence of atropine, and this effect was blocked by methiothepin, a non-selective 5-HT receptor antagonist, in the presence of atropine. Tritium 79-81 5-hydroxytryptamine receptor 1B Oryctolagus cuniculus 14-20 11020478-2 2000 The 5-HT(2A) receptor was characterized by the binding of [3H]MDL 100,907 (R(+)-alpha-(2, 3-dimethoxyphenyl)-1-[2-(4-fluorophenylethyl)]-4-piperidine-methan ol) to cortical membranes and the 5-HT(2C) receptor by the binding of [3H]mesulergine in the presence of the selective 5-HT(2A) receptor ligand spiperone. Tritium 59-61 5-hydroxytryptamine receptor 2A Oryctolagus cuniculus 4-21 16472782-7 2006 Pharmacological results revealed that CCK-8S suppressed 3H-DAMGO binding in cortical membranes of ovariectomized rats. Tritium 56-58 cholecystokinin Rattus norvegicus 38-41 16472782-9 2006 Antagonism of the CCK1 receptor by JMV-179 blocked both CCK-8S and JMV-180 suppression of 3H-DAMGO binding. Tritium 90-92 cholecystokinin Rattus norvegicus 18-21 16524172-4 2006 RESULTS: The expression of caspase-8 in cortex and hippocampus could be detected in 30 min after injury, increased significantly in 3h, reached apex in 1d after injury, remained 4d before decreased. Tritium 132-134 caspase 8 Rattus norvegicus 27-36 12667959-5 2003 METHODS AND RESULTS: [3H]Sphingosine (Sph), incorporated into CASMCs, was converted to [3H]Sph-1-P intracellularly, but its stimulation-dependent formation and extracellular release were not observed. Tritium 22-24 ankyrin 1 Homo sapiens 91-96 12667959-10 2003 Finally, exogenously-added [3H]Sph-1-P underwent a rapid degradation. Tritium 28-30 ankyrin 1 Homo sapiens 31-36 12565706-6 2003 A polyclonal antiserum raised against SR-BI significantly decreased cell-association of [3H]CE-HDL3 in HUH-7 and WRL-68. Tritium 89-91 scavenger receptor class B member 1 Homo sapiens 38-43 12565706-6 2003 A polyclonal antiserum raised against SR-BI significantly decreased cell-association of [3H]CE-HDL3 in HUH-7 and WRL-68. Tritium 89-91 MIR7-3 host gene Homo sapiens 103-108 11020478-8 2000 Binding of [3H]MDL 100,907 to the 5-HT(2A) receptor and of [3H]mesulergine to the 5-HT(2C) receptor was confirmed by displacement studies with highly selective 5-HT(2A) and 5-HT(2C) receptor ligands. Tritium 12-14 5-hydroxytryptamine receptor 2A Oryctolagus cuniculus 34-51 11023996-3 2000 CYP26 (P450RAI), a novel cytochrome P450, is expressed during embryonic development, induced by all-trans RA, and capable of catalyzing the oxidation of [3H]RA to polar retinoids including 4-oxo-RA. Tritium 154-156 cytochrome P450, family 26, subfamily a, polypeptide 1 Rattus norvegicus 0-5 12743621-0 2003 CCK-8 modulates GABA-stimulated [3H]-flunitrazepam binding in vitro. Tritium 33-35 cholecystokinin Rattus norvegicus 0-3 16563187-8 2006 Median values of [3H]thymidine incorporation were higher in SLE T cells with ICOS co-stimulation than in normal T cells, and the difference between inactive SLE patients and normal control individuals achieved statistical significance. Tritium 18-20 inducible T cell costimulator Homo sapiens 77-81 10951224-6 2000 Approximately 20-30% of the fluorescent labeled NPY and a tritium-marked NPY were resistant to acid removal of cell surface-bound ligands indicating internalization. Tritium 58-65 neuropeptide Y Homo sapiens 73-76 16084120-4 2006 Ciclesonide (3 microM) significantly inhibited ICAM-1 expression by stimulated HBECs, already after 3h and still after 48 h culture (p < 0.01). Tritium 100-102 intercellular adhesion molecule 1 Homo sapiens 47-53 12654340-6 2003 In addition, dramatic increases in [(3)H]rauwolscine binding were seen in the nerve layer of the olfactory bulb, the molecular layer of the cerebellum, and the ventricular system of alpha(2C)-AR-overexpressing mice, representing "ectopic" alpha(2C)-AR expression. Tritium 35-40 adrenergic receptor, alpha 2c Mus musculus 182-190 12654340-6 2003 In addition, dramatic increases in [(3)H]rauwolscine binding were seen in the nerve layer of the olfactory bulb, the molecular layer of the cerebellum, and the ventricular system of alpha(2C)-AR-overexpressing mice, representing "ectopic" alpha(2C)-AR expression. Tritium 35-40 adrenergic receptor, alpha 2c Mus musculus 239-247 12856821-1 2003 Rat behavior in the open field and the conditioned fear response test was correlated with specific binding of the alpha4beta2 nicotinic acetylcholine receptor (nAChR) agonist, [3H]-pibatidine, assayed in different brain structures by autoradiography. Tritium 177-180 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 160-165 10936212-7 2000 The receptor binding characteristics of the native and mutant receptors were investigated using the novel 5-HT(1A) receptor antagonist [(3)H]NAD-299. Tritium 136-140 5-hydroxytryptamine receptor 1A Homo sapiens 106-123 12509984-5 2003 The experimental assays using 3H-labeled substrate demonstrate that the purified HBV preS1-HT can be effectively N-myristoylated by recombinant human protein N-myristoyltransferase (NMT) in vitro. Tritium 30-32 large envelope protein;middle envelope protein;small envelope protein Hepatitis B virus 85-90 16204232-2 2005 Here we have characterized the intracellular lipidation of newly synthesized apoA-I, in primary hepatocytes cultured with [3H]choline to label choline-phospholipids, low density lipoprotein-[3H]cholesterol to label the cell surface, or [3H]mevalonate to label de novo synthesized cholesterol. Tritium 123-125 apolipoprotein A-I Mus musculus 77-83 16204232-2 2005 Here we have characterized the intracellular lipidation of newly synthesized apoA-I, in primary hepatocytes cultured with [3H]choline to label choline-phospholipids, low density lipoprotein-[3H]cholesterol to label the cell surface, or [3H]mevalonate to label de novo synthesized cholesterol. Tritium 191-193 apolipoprotein A-I Mus musculus 77-83 10925268-4 2000 This in vitro transfection of p21 resulted in the inhibition of spontaneous and mitogen-induced cellular proliferation ([3H]thymidine uptake) and also augmented the antiproliferative effects of cyclosporine. Tritium 121-123 H3 histone pseudogene 16 Homo sapiens 30-33 16231004-3 2005 Compared to controls, the use of adenosine deaminase (ADA), 100 microM 5"-guanylylimidodiphosphate (Gpp(NH)p) or urea each caused equivalent increases in specific [3H]DPCPX binding. Tritium 164-166 adenosine deaminase Homo sapiens 33-52 16231004-3 2005 Compared to controls, the use of adenosine deaminase (ADA), 100 microM 5"-guanylylimidodiphosphate (Gpp(NH)p) or urea each caused equivalent increases in specific [3H]DPCPX binding. Tritium 164-166 adenosine deaminase Homo sapiens 54-57 12457739-0 2002 Pentylenetetrazol-kindling of seizures selectively decreases [3H]-citalopram binding in the CA-3 area of rat hippocampus. Tritium 62-64 carbonic anhydrase 3 Rattus norvegicus 92-96 12457739-3 2002 A statistically significant and selective reduction in the binding of [3H]-citalopram was found in the CA3 field of the hippocampus (P=0.009), and a similar tendency, close to the significance level, in the dentate gyrus (P=0.05). Tritium 71-73 carbonic anhydrase 3 Rattus norvegicus 103-106 12466246-4 2002 The electrically evoked tritium overflow from guinea-pig and mouse striatal slices and guinea-pig retinal discs preincubated with [(3)H]-dopamine was inhibited by nociceptin/orphanin FQ (pEC(50) 7.9, 7.6 and 8.6; E(max) 30, 50 and 55%). Tritium 24-31 prepronociceptin Mus musculus 174-185 12202492-3 2002 Studies on the regulation of ABCA1 by the liver X receptor agonist 24(S)OH-cholesterol revealed increased ABCA1 expression and apoA-I-dependent [3H]cholesterol efflux from pBCEC. Tritium 145-147 ATP binding cassette subfamily A member 1 Homo sapiens 29-34 12202492-4 2002 In unpolarized pBCEC, high density lipoprotein, subclass 3 (HDL3)-dependent [3H]cholesterol efflux, was unaffected by 24(S)OH-cholesterol treatment but was enhanced 5-fold in SR-BI overexpressing pBCEC. Tritium 77-79 scavenger receptor class B member 1 Homo sapiens 175-180 16239589-7 2005 Stimulation of endogenous and overexpressed beta2AR on endothelial cells in vitro was found to regulate cell number by inducing proliferation and [3H]-thymidine incorporation through means of extracellular receptor-activated kinase and vascular endothelial growth factor. Tritium 147-149 adrenoceptor beta 2 Homo sapiens 44-51 16256979-0 2005 CRTH2-specific binding characteristics of [3H]ramatroban and its effects on PGD2-, 15-deoxy-Delta12, 14-PGJ2- and indomethacin-induced agonist responses. Tritium 43-45 prostaglandin D2 receptor 2 Homo sapiens 0-5 10871301-0 2000 Adrenal glucocorticoids modulate [3H]cyclic AMP binding to protein kinase A (PKA), cyclic AMP-dependent PKA activity, and protein levels of selective regulatory and catalytic subunit isoforms of PKA in rat brain. Tritium 34-36 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 59-75 16179596-6 2005 Antisense SOX18 inhibited DNA synthesis ([3H]thymidine incorporation) and vascular cell growth. Tritium 42-44 SRY-box transcription factor 18 Homo sapiens 10-15 12406087-4 2002 Autonomous growth and IL-2- or IL-15-responsive growth activities of ATL cells were measured by [3H]-thymidine incorporation after 24 h cultures in vitro. Tritium 97-99 interleukin 15 Homo sapiens 31-36 12296647-3 2002 METHODS: The authors examined the cytotoxic activity of IFNbeta-stimulated peritoneal macrophages in glioma cells labeled with [3H]thymidine. Tritium 128-130 interferon beta 1, fibroblast Mus musculus 56-63 10871301-0 2000 Adrenal glucocorticoids modulate [3H]cyclic AMP binding to protein kinase A (PKA), cyclic AMP-dependent PKA activity, and protein levels of selective regulatory and catalytic subunit isoforms of PKA in rat brain. Tritium 34-36 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 77-80 16026763-8 2005 PAR3 antisense oligonucleotides substantially inhibit thrombin-stimulated [3H]thymidine incorporation and PtdIns(3,4,5)P3 generation but had no effect on thrombin-induced phosphoinositide hydrolysis. Tritium 75-77 coagulation factor II, thrombin Bos taurus 54-62 10737622-5 2000 PARP enzymatic activity was investigated by measuring nuclear [3H]NAD incorporation. Tritium 63-65 poly (ADP-ribose) polymerase 1 Rattus norvegicus 0-4 16111674-1 2005 Searching for potent and subtype selective parent structures of the murine gamma-aminobutyric acid (GABA) transporter subtypes mGAT3 and mGAT4 a series of amino acids was characterised in a uniform [3H]GABA uptake test system based on transiently expressed mGAT1-4. Tritium 199-201 solute carrier family 6 (neurotransmitter transporter, GABA), member 11 Mus musculus 127-132 12020611-4 2002 Increased densities of binding sites for the PTBR ligand [3H]-PK11195 are also observed in these conditions as well as in brains of animals with acute liver failure, congenital urea cycle disorders and in patients who died in hepatic coma. Tritium 58-60 translocator protein Homo sapiens 45-49 10733005-0 2000 Reduced [3H]IP3 binding but unchanged IP3 receptor levels in the rat hippocampus CA1 region following transient global ischemia and tolerance induction. Tritium 9-11 carbonic anhydrase 1 Rattus norvegicus 81-84 12128156-4 2002 The c-fos mRNA levels were increased in intact male and female rats after 30min or 3h of one single cocaine injection and after 14 days of single daily cocaine injections. Tritium 83-85 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 4-9 12128010-0 2002 Receptor-mediated internalization of [3H]-neurotensin in synaptosomal preparations from rat neostriatum. Tritium 38-40 neurotensin Rattus norvegicus 42-53 15951431-8 2005 Although ABCA1-dPEST showed higher cholesterol efflux than did ABCA1-wt following cell surface labeling ([3H]cholesterol/acLDL in the absence of SR-A co-transfection), it showed impaired cholesterol efflux after late endosomal labeling ([3H]cholesterol/acLDL in the presence of SR-A). Tritium 106-108 ATP binding cassette subfamily A member 1 Homo sapiens 9-14 15951431-8 2005 Although ABCA1-dPEST showed higher cholesterol efflux than did ABCA1-wt following cell surface labeling ([3H]cholesterol/acLDL in the absence of SR-A co-transfection), it showed impaired cholesterol efflux after late endosomal labeling ([3H]cholesterol/acLDL in the presence of SR-A). Tritium 238-240 ATP binding cassette subfamily A member 1 Homo sapiens 9-14 12126740-2 2002 In this study, oxytocin 100-1000 pmol/l increased cell proliferation of primary cultures of hOB cells, measured by [3H]thymidine incorporation, (P<0.01). Tritium 116-118 oxytocin/neurophysin I prepropeptide Homo sapiens 15-23 10733005-6 2000 However, [3H]IP3 binding was significantly reduced after both damage- and tolerance-inducing ischemia in the hippocampus CA1 region. Tritium 10-12 carbonic anhydrase 1 Rattus norvegicus 121-124 12126740-3 2002 In human osteosarcoma cell-line (SaOS-2), oxytocin 100 pmol/l increased cell proliferation (measured by [3H]thymidine incorporation and a commercially available kit) and protein synthesis ([3H]proline incorporation) (P<0.05). Tritium 105-107 oxytocin/neurophysin I prepropeptide Homo sapiens 42-50 12126740-3 2002 In human osteosarcoma cell-line (SaOS-2), oxytocin 100 pmol/l increased cell proliferation (measured by [3H]thymidine incorporation and a commercially available kit) and protein synthesis ([3H]proline incorporation) (P<0.05). Tritium 190-192 oxytocin/neurophysin I prepropeptide Homo sapiens 42-50 16152973-2 2005 All test compounds competed with [3H]E2 for binding to a recombinant Xenopus estrogen receptor (xER) with the following relative affinities: E2 > alpha-ZEA > 4-t-OP > GEN. Tritium 34-36 estrogen receptor 1 S homeolog Xenopus laevis 69-94 10686583-1 2000 [(3)H]-Arachidonic acid-labelled rat T lymphocytes released radioactivity extracellularly when stimulated by the calcium ionophore A23187 or by monoclonal antibodies to some cell surface structures (CD2, CD5, CD11a, CD18, CD54, T-cell receptor) but not to others (CD49d, CD62L); release was greater with the calcium ionophore. Tritium 1-5 integrin subunit alpha 4 Homo sapiens 264-269 16152973-2 2005 All test compounds competed with [3H]E2 for binding to a recombinant Xenopus estrogen receptor (xER) with the following relative affinities: E2 > alpha-ZEA > 4-t-OP > GEN. Tritium 34-36 estrogen receptor 1 S homeolog Xenopus laevis 96-99 15784655-3 2005 YC-1 inhibited the ability of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in a dose-dependent manner to induce proliferation, migration, and tube formation in human umbilical vascular endothelial cells; these outcomes were evaluated using [3H]thymidine incorporation, transwell chamber, and Matrigel-coated slide assays, respectively. Tritium 280-282 RNA binding motif single stranded interacting protein 1 Homo sapiens 0-4 15657301-3 2005 [3H]AVP ligand binding documented two- to threefold greater density of V1a receptors in euvolemic SHR vs. Wistar-Kyoto (WKY) rats. Tritium 1-3 arginine vasopressin receptor 1A Rattus norvegicus 71-74 15854583-2 2005 In the present study, we have demonstrated that atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP) inhibited [3H]isatin binding to rat brain sections and isolated membrane fractions. Tritium 129-131 natriuretic peptide A Rattus norvegicus 48-74 15854583-2 2005 In the present study, we have demonstrated that atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP) inhibited [3H]isatin binding to rat brain sections and isolated membrane fractions. Tritium 129-131 natriuretic peptide C Rattus norvegicus 85-111 15854583-2 2005 In the present study, we have demonstrated that atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP) inhibited [3H]isatin binding to rat brain sections and isolated membrane fractions. Tritium 129-131 natriuretic peptide C Rattus norvegicus 113-116 12062473-8 2002 The IC(50) values of TRH-like peptides for displacement of [3H]TRH from TRHR2 were TRH<<<(Leu(2)-, Phe(2)-TRH)<(Gln(2)-, Ser(2)-TRH)<<(Val(2)-, Tyr(2)-, Arg(2)-, Thr(2)-, and Glu(2)-TRH). Tritium 60-62 motilin receptor Rattus norvegicus 72-77 11983494-6 2002 Injection of increasing mass of [(3)H]-20 alpha-DHP to ovariectomized/adrenalectomized (ovx/adx) rats results in a linear increase in (3)H-steroid 10 min post injection in all tissues. Tritium 34-37 dihydropyrimidinase Rattus norvegicus 48-51 10686583-1 2000 [(3)H]-Arachidonic acid-labelled rat T lymphocytes released radioactivity extracellularly when stimulated by the calcium ionophore A23187 or by monoclonal antibodies to some cell surface structures (CD2, CD5, CD11a, CD18, CD54, T-cell receptor) but not to others (CD49d, CD62L); release was greater with the calcium ionophore. Tritium 1-5 selectin L Homo sapiens 271-276 11814461-4 2002 METHODS: Activities of GALC towards galactosylceramide (GC) and galactosylsphingosine (psychosine; PS) were determined in white blood cells and cultured fibroblasts derived from GLD patients and controls using tritium-labelled natural substrates. Tritium 210-217 galactosylceramidase Homo sapiens 23-27 10720957-8 2000 Stable overexpression of cPLA2 in GEC amplified the C5b-9-induced increases in free [3H]AA, while analogous overexpression of group IIA sPLA2 had no effect. Tritium 85-87 phospholipase A2 group IVA Rattus norvegicus 25-30 11893007-5 2002 Receptor sites were evaluated using an in vitro binding assay and the selective PTBR ligand [3H]PK11195. Tritium 93-95 translocator protein Homo sapiens 80-84 15769477-3 2005 In our study we investigated the presence of perinuclear/nuclear PBR in different hepatic tumor cell lines with regard to binding to [3H] PK 11195 and protein analysis. Tritium 134-136 translocator protein Homo sapiens 65-68 15368538-4 2005 H2O2 not only caused a rapid increase in the phosphorylation of p44/42 MAPK, but also promoted the translocation of cytosolic phospholipase A2 (cPLA2) from the cytosolic to particulate fraction, and stimulated cellular [3H]-arachidonic acid (AA) release. Tritium 220-222 phospholipase A2 group IVA Homo sapiens 144-149 11820798-1 2002 In HEK 293 cells expressing the human dopamine transporter (DAT), a 10-min incubation with 10 microM cocaine followed by extensive washing resulted in a 30% increase in [3H]dopamine (DA) uptake as well as an increase in cell surface DAT in biotinylation experiments. Tritium 170-172 solute carrier family 6 member 3 Homo sapiens 38-58 10737702-2 2000 All myeloma cell lines express a PAF acetylhydrolase activity and metabolize [3H]PAF and [3H]lyso PAF in 1-alkyl-2-acyl analogue of phosphatidylcholine. Tritium 78-80 PCNA clamp associated factor Homo sapiens 81-84 11820798-1 2002 In HEK 293 cells expressing the human dopamine transporter (DAT), a 10-min incubation with 10 microM cocaine followed by extensive washing resulted in a 30% increase in [3H]dopamine (DA) uptake as well as an increase in cell surface DAT in biotinylation experiments. Tritium 170-172 solute carrier family 6 member 3 Homo sapiens 60-63 11835393-5 2002 Plasminogen-dependent [3H]-collagen IV degradation was inhibited by inhibitor of uPA (amiloride) and MMP (phenanthroline) and by antibodies against uPA or MMP-9 or alphavbeta6 integrin, indicating the involvement of alphavbeta6 integrin, uPA and MMP-9 in the process. Tritium 23-25 matrix metallopeptidase 9 Homo sapiens 155-160 15500446-4 2005 Expression of inactive CK2alpha" (decreased CK2alpha" activity) increased the number of binding sites (approximately 1.5-fold) for the es-specific probe [3H]NBMPR ([3H]nitrobenzylthioinosine), and increased (approximately 1.8-fold) the V(max) for 2-chloro[3H]adenosine of the NBMPR-sensitive (es) nucleoside transporter. Tritium 154-156 casein kinase 2 alpha 2 Homo sapiens 23-31 15500446-4 2005 Expression of inactive CK2alpha" (decreased CK2alpha" activity) increased the number of binding sites (approximately 1.5-fold) for the es-specific probe [3H]NBMPR ([3H]nitrobenzylthioinosine), and increased (approximately 1.8-fold) the V(max) for 2-chloro[3H]adenosine of the NBMPR-sensitive (es) nucleoside transporter. Tritium 154-156 casein kinase 2 alpha 2 Homo sapiens 44-52 11835393-5 2002 Plasminogen-dependent [3H]-collagen IV degradation was inhibited by inhibitor of uPA (amiloride) and MMP (phenanthroline) and by antibodies against uPA or MMP-9 or alphavbeta6 integrin, indicating the involvement of alphavbeta6 integrin, uPA and MMP-9 in the process. Tritium 23-25 matrix metallopeptidase 9 Homo sapiens 246-251 10640614-6 2000 The activity of 3H-IGF-1 signal in the potentially injured cortex was compared between two treatment groups using image analysis. Tritium 16-18 insulin-like growth factor 1 Rattus norvegicus 19-24 12120007-9 2002 In contrast, inhibition of both protein kinases leads to reduced cholecystokinin-stimulated [3H]thymidine incorporation rates. Tritium 93-95 cholecystokinin Rattus norvegicus 65-80 15537862-9 2005 Finally, we showed that LIF increased [3H]-thymidine incorporation in spermatogonia in microdissected, cultured seminiferous tubules. Tritium 39-41 LIF, interleukin 6 family cytokine Rattus norvegicus 24-27 10640614-7 2000 The regional distribution and cellular localisation of 3H-IGF-1 were examined autoradiographically in potentially injured hemispheres at 0.5 and 6 h after administration. Tritium 55-57 insulin-like growth factor 1 Rattus norvegicus 58-63 10600650-6 2000 [(3)H]Palmitate incorporation into unbound SNAP-25 was negligible, but was stimulated 100-fold when SNAP-25 was present in the SNARE complex. Tritium 0-5 synaptosome associated protein 25 Homo sapiens 100-107 15710410-1 2005 In tritium-hydrogen exchange experiments, the large GroEL substrate Rubisco was unfolded and exchanged in urea/acid/tritiated water, then diluted into either protic buffer or protic buffer containing GroEL. Tritium 3-10 heat shock protein family D (Hsp60) member 1 Homo sapiens 52-57 11788749-9 2002 AtOPT4 expressed in S. cerevisiae mediated the uptake of KLG-[3H]L. Similarly, expression of five of the seven AtOPT proteins expressed in yeast conferred the ability to uptake tetra- and pentapeptides as measured by growth. Tritium 62-64 oligopeptide transporter 4 Arabidopsis thaliana 0-6 10803925-3 2000 METHODS: TS enzymatic activity was measured by both an in situ tritium release assay using both the H630 colon cancer cell line and the CEM human leukemia cell line, and by a radiolabelled in vitro assay using partially purified human TS as the enzyme source. Tritium 63-70 thymidylate synthetase Homo sapiens 9-11 11489908-6 2001 Binding of the NK2 receptor antagonist [3H]SR 48968 and of 125I-NKA was used to characterize additional receptor mutants. Tritium 40-42 tachykinin receptor 2 Homo sapiens 15-27 11550977-4 2001 RESULTS: GM-CSF (6-100 U/ml) inhibited (30%) [3H]-thymidine incorporation into DNA, and, in contrast, stimulated up to 3.6- and 2-fold [35S]SO4 and [3H]-proline incorporation into glycosaminoglycan side chains and collagen molecules, respectively. Tritium 46-48 colony stimulating factor 2 Rattus norvegicus 9-15 11550977-4 2001 RESULTS: GM-CSF (6-100 U/ml) inhibited (30%) [3H]-thymidine incorporation into DNA, and, in contrast, stimulated up to 3.6- and 2-fold [35S]SO4 and [3H]-proline incorporation into glycosaminoglycan side chains and collagen molecules, respectively. Tritium 149-151 colony stimulating factor 2 Rattus norvegicus 9-15 15576844-7 2005 Finally, purified apoC-I impaired the clearance of [3H]TG-VLDL-mimicking particles independent of apoE-mediated hepatic uptake in lactoferrin-treated mice. Tritium 52-54 apolipoprotein C-I Mus musculus 18-24 15680393-4 2005 Cell proliferation in response to SST treatment (0.04, 0.2, or 1 ng/ml) was performed by [3H]thymidine incorporation. Tritium 90-92 somatostatin Homo sapiens 34-37 15680393-11 2005 SST-treated cells showed a significant reduction in [3H]thymidine incorporation in a dose-dependent manner. Tritium 53-55 somatostatin Homo sapiens 0-3 15783071-8 2005 P-gp limited fractional absorption of [3H]-verapamil via efflux from the nasal cavity and olfactory epithelium. Tritium 39-41 phosphoglycolate phosphatase Mus musculus 0-4 15783071-9 2005 P-gp also increased the rate constants associated with [3H]-verapamil efflux 1.5- to 190-fold, depending on brain region. Tritium 56-58 phosphoglycolate phosphatase Mus musculus 0-4 15783071-10 2005 P-gp limited [3H]-verapamil uptake from the nasal cavity into brain and facilitated removal of [3H]-verapamil from brain during rostral-to-caudal distribution. Tritium 14-16 phosphoglycolate phosphatase Mus musculus 0-4 15783071-10 2005 P-gp limited [3H]-verapamil uptake from the nasal cavity into brain and facilitated removal of [3H]-verapamil from brain during rostral-to-caudal distribution. Tritium 96-98 phosphoglycolate phosphatase Mus musculus 0-4 11699943-0 2001 The role of glycineB binding site and glycine transporter (GlyT1) in the regulation of [3H]GABA and [3H]glycine release in the rat brain. Tritium 88-90 solute carrier family 6 member 9 Rattus norvegicus 59-64 10810252-0 2000 Effect of spleen-cell-conditioned medium on [3H]-choline uptake by retinal cells in vitro is mediated by IL-2. Tritium 45-47 interleukin 2 Rattus norvegicus 105-109 11699943-0 2001 The role of glycineB binding site and glycine transporter (GlyT1) in the regulation of [3H]GABA and [3H]glycine release in the rat brain. Tritium 101-103 solute carrier family 6 member 9 Rattus norvegicus 59-64 15630695-7 2005 However, quantitative autoradiographic analysis indicated enhanced 3H-AMPA binding to the CA1 stratum radiatum of the hippocampus in BEL-treated sections. Tritium 67-69 carbonic anhydrase 1 Homo sapiens 90-93 10810252-11 2000 Anti-interleukin-2 (IL-2) antibodies blocked the effect of both SCM and S-GCM on [3H]-choline uptake after 48 and 72 h. IL-2 (50 U/ml) elicited the same effect as that observed when the cells were maintained in the presence of SCM. Tritium 82-84 interleukin 2 Rattus norvegicus 5-18 16215318-6 2005 CRABP-II (un)transfected CAKI-2 cells were stimulated with all-trans RA (ATRA) and 9-cis RA, and their antiproliferative effects were evaluated using 3H-thymidine-proliferation assays. Tritium 150-152 cellular retinoic acid binding protein 2 Homo sapiens 0-8 11434908-11 2001 Thus, rs2-2.HA encodes a high-affinity [3H](+)-pentazocine binding site with characteristics of a rat sigma1 receptor. Tritium 40-43 sigma non-opioid intracellular receptor 1 Rattus norvegicus 102-117 11425508-4 2001 [3H]Ro 25-6981 (an NMDA antagonist selective for NR1/NR2B assembly) specific binding and mRNA levels of NMDA receptor subunits 1 and 2B decreased in CA1 after ovariectomy. Tritium 1-3 glutamate ionotropic receptor NMDA type subunit 2B Rattus norvegicus 53-57 10810252-11 2000 Anti-interleukin-2 (IL-2) antibodies blocked the effect of both SCM and S-GCM on [3H]-choline uptake after 48 and 72 h. IL-2 (50 U/ml) elicited the same effect as that observed when the cells were maintained in the presence of SCM. Tritium 82-84 interleukin 2 Rattus norvegicus 20-24 10810252-11 2000 Anti-interleukin-2 (IL-2) antibodies blocked the effect of both SCM and S-GCM on [3H]-choline uptake after 48 and 72 h. IL-2 (50 U/ml) elicited the same effect as that observed when the cells were maintained in the presence of SCM. Tritium 82-84 interleukin 2 Rattus norvegicus 120-124 10629765-5 1999 [3H]AA release was inhibited by arachidonyl trifluoromethyl ketone, a specific inhibitor of cytosolic PLA2, and cytosolic PLA2 protein with a molecular mass of 100 kDa was detected by immunoblotting. Tritium 1-3 phospholipase A2 group IB Rattus norvegicus 102-106 11431013-2 2001 The radiolabeled DAT ligand [3H]GBR12935 was bound to peripheral lymphocytes in a manner consistent with the specific binding to a dopamine uptake system, with a dissociation constant similar to that found in striatum, but with a lower density of binding sites. Tritium 29-31 solute carrier family 6 member 3 Homo sapiens 17-20 16293216-5 2004 Mouse IL-21 expression was analyzed by Western blotting and its activities were detected by 3H-TdR incorporation and MTT assay. Tritium 92-94 interleukin 21 Mus musculus 6-11 15231870-6 2004 Analysis of mGluR4/mGluR6 receptor chimeras revealed that replacement of the first 35 amino acids of mGluR6 with the first 50 amino acids of mGluR4 was sufficient to impart [3H]L-AP4 binding to mGluR6. Tritium 174-176 glutamate receptor, ionotropic, kainate 2 (beta 2) Mus musculus 19-25 15231870-6 2004 Analysis of mGluR4/mGluR6 receptor chimeras revealed that replacement of the first 35 amino acids of mGluR6 with the first 50 amino acids of mGluR4 was sufficient to impart [3H]L-AP4 binding to mGluR6. Tritium 174-176 glutamate receptor, ionotropic, kainate 2 (beta 2) Mus musculus 101-107 15231870-6 2004 Analysis of mGluR4/mGluR6 receptor chimeras revealed that replacement of the first 35 amino acids of mGluR6 with the first 50 amino acids of mGluR4 was sufficient to impart [3H]L-AP4 binding to mGluR6. Tritium 174-176 glutamate receptor, ionotropic, kainate 2 (beta 2) Mus musculus 101-107 10629765-5 1999 [3H]AA release was inhibited by arachidonyl trifluoromethyl ketone, a specific inhibitor of cytosolic PLA2, and cytosolic PLA2 protein with a molecular mass of 100 kDa was detected by immunoblotting. Tritium 1-3 phospholipase A2 group IB Rattus norvegicus 122-126 15102929-2 2004 Cofunction of hDAT and hVMAT2 caused greater retention of [3H]DA at 20 min (37 degrees C), or 45 min (22 degrees C) compared with cells that were treated with dihydrotetrabenazene (DHTB) to block the hVMAT2. Tritium 59-61 solute carrier family 6 member 3 Homo sapiens 14-18 11445189-6 2001 Efflux of pre-loaded [3H]-glycine from GlyT-1c cells was increased by glycine or sarcosine, whereas NFPS had no effect on its own but blocked the effects of glycine or sarcosine. Tritium 22-24 solute carrier family 6 member 9 Rattus norvegicus 39-45 10602484-7 1999 Unexpectedly, IL-4"s enhancement of PDGF-induced [3H]thymidine incorporation was greatly diminished in Stat6-/-, but not wild-type fibroblasts. Tritium 50-52 interleukin 4 Mus musculus 14-18 11453166-5 2001 An obvious difference between hippocampal slice cultures and hippocampi in situ was a changed distribution of binding sites among the hippocampal areas showing a relative increase of [3H]MK-801 and [3H]AMPA binding sites in CA3 as compared to CA1 and to the dentate gyrus in the cultures. Tritium 184-186 carbonic anhydrase 3 Rattus norvegicus 224-227 11453166-5 2001 An obvious difference between hippocampal slice cultures and hippocampi in situ was a changed distribution of binding sites among the hippocampal areas showing a relative increase of [3H]MK-801 and [3H]AMPA binding sites in CA3 as compared to CA1 and to the dentate gyrus in the cultures. Tritium 199-201 carbonic anhydrase 3 Rattus norvegicus 224-227 11697575-1 2001 The binding characteristics of the pBR (peripheral benzodiazepine receptor) inverse agonist, [3H]-Ro 5-4864, were examined in patients diagnosed as generalized anxiety disorder. Tritium 94-96 translocator protein Homo sapiens 35-38 11697575-1 2001 The binding characteristics of the pBR (peripheral benzodiazepine receptor) inverse agonist, [3H]-Ro 5-4864, were examined in patients diagnosed as generalized anxiety disorder. Tritium 94-96 translocator protein Homo sapiens 40-74 15245435-5 2004 Using RT-PCR, immunofluorescence and radioligand binding with the beta2-AR antagonist (-)-[3H]CGP 12177, we show here that human melanocytes express functional beta2-AR (4230 receptors per cell) with a Bmax at 129.3 and a KD of 3.19 nM but lack beta1-AR expression. Tritium 91-93 adrenoceptor beta 2 Homo sapiens 160-168 10746169-4 1999 [3H]Azidopine photoaffinity labeling demonstrated that itraconazole, vinblastine, daunorubicin and doxorubicin showed higher binding ability for P-gp compared with digoxin, suggesting the following results were via P-gp. Tritium 1-3 phosphoglycolate phosphatase Homo sapiens 145-149 14757131-4 2004 The kappa-opioid receptor antagonist nor-binaltorphimine had a four-fold higher affinity for [3H]U69,593-labeled receptors than for [3H]bremazocine-labeled receptors. Tritium 94-96 opioid receptor kappa 1 Homo sapiens 4-25 14757131-4 2004 The kappa-opioid receptor antagonist nor-binaltorphimine had a four-fold higher affinity for [3H]U69,593-labeled receptors than for [3H]bremazocine-labeled receptors. Tritium 133-135 opioid receptor kappa 1 Homo sapiens 4-25 11465634-3 2001 METHODS: Since 5-HT2C receptors are positively coupled to phospholipase C (PLC), their activation was determined by depletion of membrane-bound pools of pre-labelled [3H]phosphotidylinositol ([3H]PI). Tritium 167-169 5-hydroxytryptamine receptor 2C Homo sapiens 15-21 11465634-3 2001 METHODS: Since 5-HT2C receptors are positively coupled to phospholipase C (PLC), their activation was determined by depletion of membrane-bound pools of pre-labelled [3H]phosphotidylinositol ([3H]PI). Tritium 193-195 5-hydroxytryptamine receptor 2C Homo sapiens 15-21 10746169-4 1999 [3H]Azidopine photoaffinity labeling demonstrated that itraconazole, vinblastine, daunorubicin and doxorubicin showed higher binding ability for P-gp compared with digoxin, suggesting the following results were via P-gp. Tritium 1-3 phosphoglycolate phosphatase Homo sapiens 215-219 11409646-6 2001 The dose-dependent inhibition of the up-take of [3H]-estradiol-17beta-D-glucuronide and [3H]-2,4-dinitrophenyl-S-glutathione, which are good substrates for cMOAT, in canalicular membrane vesicles (CMVs) prepared from Sprague-Dawley rats was determined in the presence of trandolaprilat and temocaprilat. Tritium 49-51 ATP binding cassette subfamily C member 2 Rattus norvegicus 156-161 14742684-4 2004 Furthermore, MMB and PRQ, but not OME, were capable of transforming cytosolic AhR to a DNA-binding form and displacing AhR-bound [3H]TCDD in rat hepatic cytosol in vitro. Tritium 130-132 aryl hydrocarbon receptor Rattus norvegicus 119-122 14742684-6 2004 Comparable affinities of [3H]TCDD-binding to the wild-type and the Y320F mutant Gal4-proteins, expressed in human embryonic kidney 293 cells, were obtained in the ligand-binding assay. Tritium 26-28 galectin 4 Homo sapiens 80-84 10746169-8 1999 The apical-to-basal transport of [3H]vinblastine, [3H]daunorubicin and [14C]doxorubicin was increased by itraconazole, and this was reasonably explained by the inhibition of P-gp, and partly also by the increase of their intracellular concentration via the inhibition of P-gp. Tritium 33-36 phosphoglycolate phosphatase Homo sapiens 174-178 14742684-7 2004 In contrast, the competition of receptor-bound [3H]TCDD by PRQ was absent from Gal4-Y320F but not from Gal4-AhR cell extracts. Tritium 48-50 galectin 4 Homo sapiens 79-83 10746169-8 1999 The apical-to-basal transport of [3H]vinblastine, [3H]daunorubicin and [14C]doxorubicin was increased by itraconazole, and this was reasonably explained by the inhibition of P-gp, and partly also by the increase of their intracellular concentration via the inhibition of P-gp. Tritium 33-36 phosphoglycolate phosphatase Homo sapiens 271-275 10746169-8 1999 The apical-to-basal transport of [3H]vinblastine, [3H]daunorubicin and [14C]doxorubicin was increased by itraconazole, and this was reasonably explained by the inhibition of P-gp, and partly also by the increase of their intracellular concentration via the inhibition of P-gp. Tritium 34-36 phosphoglycolate phosphatase Homo sapiens 174-178 10746169-8 1999 The apical-to-basal transport of [3H]vinblastine, [3H]daunorubicin and [14C]doxorubicin was increased by itraconazole, and this was reasonably explained by the inhibition of P-gp, and partly also by the increase of their intracellular concentration via the inhibition of P-gp. Tritium 34-36 phosphoglycolate phosphatase Homo sapiens 271-275 10589688-5 1999 In addition, exogenous Gas6 phosphorylated Axl, activated extracellular signal-regulated kinase, and stimulated [3H]-thymidine incorporation in mouse mesangial cells. Tritium 113-115 growth arrest specific 6 Mus musculus 23-27 14592853-3 2004 METHODS AND RESULTS: Nicotine or Ang II-stimulated [3H]thymidine incorporation and c-fos expression in adult rat aortic VSMC and adventitial fibroblast. Tritium 52-54 angiogenin Rattus norvegicus 33-36 15161336-1 2004 OBJECTIVE: The binding and encapsulation of [3H] pGL3 luciferase reporter plasmid DNA by red blood cell (RBC) ghosts, intended as a vehicle for transfection and ultimately for gene therapy, were studied using two methods for DNA compaction. Tritium 45-47 succinate dehydrogenase complex subunit C Homo sapiens 49-53 15161336-5 2004 An alternative approach using polyethylene glycol6000 at a final concentration of 15% (weight/volume) was used to collapse [3H] pGL3 DNA in the presence of 0.025M MgCl2. Tritium 124-126 succinate dehydrogenase complex subunit C Homo sapiens 128-132 10608276-5 1999 Several compounds known to interact with the GluR5 kainate receptor inhibited [3H]ATPA binding with potencies similar to those obtained for competition of [3H]kainate binding to GluR5. Tritium 79-81 glutamate ionotropic receptor kainate type subunit 1 Rattus norvegicus 45-50 15161336-7 2004 Uptake of [3H] pGL3 DNA by the ghosts was approximately 20% of the input amount of DNA. Tritium 11-13 succinate dehydrogenase complex subunit C Homo sapiens 15-19 10608276-5 1999 Several compounds known to interact with the GluR5 kainate receptor inhibited [3H]ATPA binding with potencies similar to those obtained for competition of [3H]kainate binding to GluR5. Tritium 79-82 glutamate ionotropic receptor kainate type subunit 1 Rattus norvegicus 45-50 10564737-3 1999 In this study, the binding of [3H]Ro41-1049 to MAO-A was measured by quantitative autoradiography at multiple levels along the rostral-caudal axis of the noradrenergic locus coeruleus from subjects with major depression and age- and postmortem interval-matched control subjects who were psychiatrically normal. Tritium 31-33 monoamine oxidase A Homo sapiens 47-52 14656380-5 2003 beta2AR density measured with [3H]dihydroalprenolol ([3H]DHA) increased either 13- or 77-fold in infected cells compared to mock infected controls depending on the culture conditions used. Tritium 31-33 adrenoceptor beta 2 Homo sapiens 0-7 10564737-4 1999 [3H]Ro41-1049 binding to MAO-A was unevenly distributed along the axis of the locus coeruleus, paralleling an uneven number of neuromelanin-containing (noradrenergic) neurons throughout the nucleus. Tritium 1-3 monoamine oxidase A Homo sapiens 25-30 14656380-6 2003 The [3H]DHA binding was to a single receptor population with a dissociation constant of 0.42 nM, as would be expected for wild-type beta2AR. Tritium 5-7 adrenoceptor beta 2 Homo sapiens 132-139 14656380-7 2003 Agonist competition assays with [3H]DHA showed the following rank order of potency: isoproterenol>epinephrine> norepinephrine, consistent with beta2AR interaction. Tritium 33-35 adrenoceptor beta 2 Homo sapiens 149-156 14663276-3 2003 MGMT activity was assayed by measuring the removal of O6-[3H] methylguanine from [3H]-methylated substrate by a tumor extract containing the enzyme. Tritium 58-60 O-6-methylguanine-DNA methyltransferase Homo sapiens 0-4 10622485-7 1999 A synthetic peptide ligand for the thrombin receptor enhanced the rate of [3H]thymidine incorporation in hOBs, indicating that thrombin-induced proliferation is mediated via the tetheric thrombin receptor. Tritium 75-77 leptin Homo sapiens 105-109 14645664-7 2003 Overall, the requirement of anionic phospholipids for the reconstitution of [3H]PDBu binding to PKD was intermediate between those of PKC-alpha and -delta. Tritium 77-79 protein kinase D1 Homo sapiens 96-99 10499523-8 1999 In addition, dominant negative mutant of H-Ras and a MAP kinase kinase (MEK-1) inhibitor (PD98059) completely blocked ET-1-induced MAP kinase activation as well as [3H]leucine and [3H]thymidine uptake. Tritium 165-167 HRas proto-oncogene, GTPase Homo sapiens 41-46 10499523-8 1999 In addition, dominant negative mutant of H-Ras and a MAP kinase kinase (MEK-1) inhibitor (PD98059) completely blocked ET-1-induced MAP kinase activation as well as [3H]leucine and [3H]thymidine uptake. Tritium 165-167 mitogen-activated protein kinase kinase 1 Homo sapiens 72-77 10499523-8 1999 In addition, dominant negative mutant of H-Ras and a MAP kinase kinase (MEK-1) inhibitor (PD98059) completely blocked ET-1-induced MAP kinase activation as well as [3H]leucine and [3H]thymidine uptake. Tritium 181-183 HRas proto-oncogene, GTPase Homo sapiens 41-46 12837768-4 2003 Of 18 mutations where hNET amino acid residues were exchanged with those of the human dopamine transporter, MrIA had increased potency for inhibition of [3H]norepinephrine uptake for three mutations (in predicted extracellular loops 3 and 4 and transmembrane domain (TMD) 8) and decreased potency for one mutation (in TMD6 and intracellular loop (IL) 3). Tritium 154-156 solute carrier family 6 member 3 Homo sapiens 86-106 10499523-8 1999 In addition, dominant negative mutant of H-Ras and a MAP kinase kinase (MEK-1) inhibitor (PD98059) completely blocked ET-1-induced MAP kinase activation as well as [3H]leucine and [3H]thymidine uptake. Tritium 181-183 mitogen-activated protein kinase kinase 1 Homo sapiens 72-77 10555184-6 1999 (3H)PAF binding to platelets of stroke patients was lower than in controls (149.58 +/- 46.11 and 212.1 +/- 10.3 receptors cell-1, respectively, p < 0.001) and was significantly correlated with infarct volume (r = -0.606, p = 0.014) and with neurological score (r = 0.527, p = 0.032). Tritium 1-3 PCNA clamp associated factor Homo sapiens 4-7 12941372-11 2003 Overall, this work shows that [3H]NFPS is a valuable tool in studying GlyT1 expression and pharmacology and that NFPS interacts with GlyT1 at a site different from the transporter translocation and ion binding sites. Tritium 31-33 solute carrier family 6 member 9 Rattus norvegicus 70-75 10484455-4 1999 Significant enhancement of [(3)H]thymidine incorporation in HASM cultures was observed only by treatment with agents (epidermal growth factor, platelet-derived growth factor, thrombin, and phorbol 12-myristate 13-acetate) that promoted a strong and sustained activation of p42/p44 MAPK. Tritium 28-32 cyclin dependent kinase 20 Homo sapiens 273-276 12951049-5 2003 HGF treatment enhanced [3H]thymidine incorporation into oval cells in a dose-dependent manner. Tritium 24-26 hepatocyte growth factor Rattus norvegicus 0-3 10654199-5 1999 The peaks of TIL 3H-TdR cytotoxicity test from 35 of 64 TIL specimens were kept from 40 to 56 days. Tritium 17-19 toll-like receptor 1 Mus musculus 56-59 12911631-9 2003 Incubation of DRG, SSN or SC tissue homogenates with [3H]cholesterol yielded the formation of radioactive metabolites including [3H]pregnenolone of which the synthesis was reduced in presence of aminogluthetimide, a P450scc inhibitor. Tritium 54-56 cytochrome P450, family 11, subfamily a, polypeptide 1 Rattus norvegicus 216-223 10513203-6 1999 3H-thymidine autoradiography revealed that in both in vivo and in vitro studies, SP + IGF-1 stimulated corneal epithelial cell proliferation, but SP or IGF-1 alone did not. Tritium 0-2 insulin-like growth factor I Oryctolagus cuniculus 86-91 12760743-1 2003 EBP (emopamil-binding protein) is a high-affinity binding protein for [3H]emopamil and belongs to the family of so-called sigma receptors. Tritium 71-73 EBP cholestenol delta-isomerase Homo sapiens 0-3 12760743-1 2003 EBP (emopamil-binding protein) is a high-affinity binding protein for [3H]emopamil and belongs to the family of so-called sigma receptors. Tritium 71-73 EBP cholestenol delta-isomerase Homo sapiens 5-29 10439474-4 1999 The binding of the GABAA ligands [3H]muscimol and [3H]SR 95531 to alpha 1 (R120K) beta 2 gamma 2s GABAA receptors was abolished but the binding affinity of the benzodiazepine receptor ligand [3H]flunitrazepam was unchanged. Tritium 51-53 adrenoceptor alpha 1D Homo sapiens 80-96 12900335-8 2003 ApoA-I overexpression led to significantly higher 3H-cholesterol in plasma, liver, and feces. Tritium 50-52 apolipoprotein A-I Mus musculus 0-6 10439474-4 1999 The binding of the GABAA ligands [3H]muscimol and [3H]SR 95531 to alpha 1 (R120K) beta 2 gamma 2s GABAA receptors was abolished but the binding affinity of the benzodiazepine receptor ligand [3H]flunitrazepam was unchanged. Tritium 51-53 adrenoceptor alpha 1D Homo sapiens 80-96 12900335-10 2003 CONCLUSIONS: Injection of 3H-cholesterol-labeled macrophage foam cells is a method of measuring reverse cholesterol transport specifically from macrophages to feces in vivo, and apoA-I overexpression promotes macrophage-specific reverse cholesterol transport. Tritium 26-28 apolipoprotein A-I Mus musculus 178-184 10432388-6 1999 Conversely, AxCAp16 inhibited EGF-stimulated [3H]-thymidine incorporation but did not change the [3H]-leucine incorporation. Tritium 46-48 epidermal growth factor Sus scrofa 30-33 12721292-3 2003 BV released from Sf9 cells infected with a recombinant baculovirus coding for human leukotriene B4 receptor (BLT1) cDNA exhibited a high level of BLT1 expression (27.3 pmol/mg of protein) and specific [3H]leukotriene B4 binding activity (Kd = 3.67 nm). Tritium 202-204 leukotriene B4 receptor Homo sapiens 84-107 12721292-3 2003 BV released from Sf9 cells infected with a recombinant baculovirus coding for human leukotriene B4 receptor (BLT1) cDNA exhibited a high level of BLT1 expression (27.3 pmol/mg of protein) and specific [3H]leukotriene B4 binding activity (Kd = 3.67 nm). Tritium 202-204 leukotriene B4 receptor Homo sapiens 109-113 10422859-8 1999 RESULTS: Estimated cell count and [3H]thymidine uptake showed that EGF stimulated cell proliferation in both corneal epithelial cells and HUVECs in a dose-dependent manner. Tritium 35-37 epidermal growth factor Homo sapiens 67-70 12606305-4 2003 Amidated gastrin (G-17), one of the major circulating forms of gastrin, induced a concentration-dependent increase in [3H]thymidine incorporation of cells in culture, with the maximum effective concentration occurring with 20 nM G-17. Tritium 119-121 PBX homeobox 2 Homo sapiens 18-22 12674335-9 2003 Low doses of PTHrP(1-34) (10(-12)-10(-9) M) increased the cell number studied by [3H]thymidine incorporation and DNA content. Tritium 82-84 parathyroid hormone-like hormone Rattus norvegicus 13-18 10479071-7 1999 Seizures from P5 to P7 led to a 22% increase in the density of [3H]MK-801 binding sites measured at P15, but did not affect NMDA receptor density or affinity at P25 or P60. Tritium 64-66 cyclin-dependent kinase inhibitor 2B Rattus norvegicus 100-103 12654502-0 2003 Exogenous brain-derived neurotrophic factor prevents postischemic downregulation of [3H]muscimol binding to GABA(A) receptors in the cortical penumbra. Tritium 85-87 brain-derived neurotrophic factor Rattus norvegicus 10-43 12623064-4 2003 Complete lack of CRABPI results in decreased intracellular [3H]RA concentrations under conditions in which external concentrations of [3H]RA are low (1-10nM) and in an altered distribution of [3H] polar metabolites of [3H]RA in the cell and in the medium. Tritium 60-62 cellular retinoic acid binding protein I Mus musculus 17-23 12623064-4 2003 Complete lack of CRABPI results in decreased intracellular [3H]RA concentrations under conditions in which external concentrations of [3H]RA are low (1-10nM) and in an altered distribution of [3H] polar metabolites of [3H]RA in the cell and in the medium. Tritium 135-137 cellular retinoic acid binding protein I Mus musculus 17-23 12623064-4 2003 Complete lack of CRABPI results in decreased intracellular [3H]RA concentrations under conditions in which external concentrations of [3H]RA are low (1-10nM) and in an altered distribution of [3H] polar metabolites of [3H]RA in the cell and in the medium. Tritium 135-137 cellular retinoic acid binding protein I Mus musculus 17-23 12623064-4 2003 Complete lack of CRABPI results in decreased intracellular [3H]RA concentrations under conditions in which external concentrations of [3H]RA are low (1-10nM) and in an altered distribution of [3H] polar metabolites of [3H]RA in the cell and in the medium. Tritium 135-137 cellular retinoic acid binding protein I Mus musculus 17-23 12623064-5 2003 Fewer [3H] polar metabolites are retained within the CRABPI(-/-) cells compared to the wild-type cells. Tritium 7-9 cellular retinoic acid binding protein I Mus musculus 53-59 11443934-2 2001 The CP structure in TMV-U1 and ts21-66 virions was probed by tritium planigraphy. Tritium 61-68 Coat protein Tobacco mosaic virus 4-6 11330432-9 2001 In terms of IEP mRNA expression and hepatic regenerative activity, a strong correlation existed between c-fos mRNA expression and [3H]thymidine incorporation (r2 = 0.851, P < 0.01) but not c-jun or c-myc mRNA expression. Tritium 131-133 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 104-109 10403620-11 1999 Inhibitor profile suggested that pyroglutamyl aminopeptidase II, but not pyroglutamyl aminopeptidase I, is responsible for [3H]-HPDKP production. Tritium 124-126 pyroglutamyl-peptidase I Rattus norvegicus 33-62 11239918-5 2001 Scatchard analysis showed that 68+/-12% of the binding sites arise from the MOR gene, 27+/-1% from the DOR gene and 14.5+/-0.2% from the KOR gene, indicating that the three known genes account for total [3H]bremazocine binding. Tritium 204-206 opioid receptor, kappa 1 Mus musculus 137-140 11315338-3 2001 Binding affinities of prepared compounds for the cloned human muscarinic M1 receptor (h-M1) were determined by radioligand binding assay using [3H]-N-methylscopolamine (NMS). Tritium 144-146 cholinergic receptor muscarinic 1 Homo sapiens 86-90 12566124-6 2003 RESULTS: Ang II-stimulated increases in markers of hypertrophy, [3H]phenylalanine incorporation (to 136+/-3% of control, n=9) and c-fos mRNA expression (4.3+/-1.4-fold, n=5), were completely prevented by each of ANP, BNP or CNP. Tritium 65-67 angiogenin Rattus norvegicus 9-12 10415911-12 1999 [3H]RO41-1049 (20 nM) binding to MAO-A was highest in the zona reticularis (196 +/- 7 fmol/mg tissue) compared to the zonae glomerulosa and fasciculata (90 +/- 12 and 116 +/- 14 fmol/mg tissue) and inner medulla (149 +/- 38 fmol/mg tissue). Tritium 1-3 monoamine oxidase A Bos taurus 33-38 12733705-2 2003 In metabolic labeling and chasing experiments with [3H]-Leu and [35S]-Met, proinsulin appeared in the medium during stimulatory secretion only from MIN6 clones expressing GHalpha1-PIM/R and, surprisingly, alpha1-PIM/R, but not from the clones of either the control or alpha1-PI. Tritium 52-54 insulin II Mus musculus 75-85 11169130-7 2001 In contrast, IL-10 induced a biphasic response; IL-10 at low concentrations (0.1 ng mL(-1)) caused a significant suppression of [3H]-uptake, whereas high concentrations (5-100 ng mL(-1)) significantly enhanced [3H] uptake. Tritium 129-131 interleukin 10 Homo sapiens 13-18 11169130-7 2001 In contrast, IL-10 induced a biphasic response; IL-10 at low concentrations (0.1 ng mL(-1)) caused a significant suppression of [3H]-uptake, whereas high concentrations (5-100 ng mL(-1)) significantly enhanced [3H] uptake. Tritium 129-131 interleukin 10 Homo sapiens 48-53 10515679-9 1999 CD30L-ETA" exhibited specific cytotoxicity against L540cy cells (IC50 = 24 ng/ml) as determined by [3H]leucine uptake assays. Tritium 100-102 TNF superfamily member 8 Homo sapiens 0-10 11169130-7 2001 In contrast, IL-10 induced a biphasic response; IL-10 at low concentrations (0.1 ng mL(-1)) caused a significant suppression of [3H]-uptake, whereas high concentrations (5-100 ng mL(-1)) significantly enhanced [3H] uptake. Tritium 211-213 interleukin 10 Homo sapiens 13-18 11169130-7 2001 In contrast, IL-10 induced a biphasic response; IL-10 at low concentrations (0.1 ng mL(-1)) caused a significant suppression of [3H]-uptake, whereas high concentrations (5-100 ng mL(-1)) significantly enhanced [3H] uptake. Tritium 211-213 interleukin 10 Homo sapiens 48-53 11811668-5 2001 The muscarinic receptor-mediated [3H]CDP-DAG response was significantly blunted in the aged rats in subiculum, CA3, and CA1. Tritium 34-36 carbonic anhydrase 3 Rattus norvegicus 111-114 12570101-5 2003 Upon EFS, NK1R-/- mice had a significant lower trachea contractility than the NK1R WT animals, accompanied with less [3H]acetylcholine release. Tritium 118-120 tachykinin receptor 1 Mus musculus 10-14 10348900-4 1999 [3H]Heparin labeled with this reagent crosslinked to antithrombin III very specifically but not to ovalbumin, as analyzed by the Bio-imaging Analyzer System (BAS, Fuji Photo Film, Tokyo). Tritium 1-3 serpin family C member 1 Homo sapiens 53-69 12490597-1 2003 The ability of a number of prostaglandin F 2 alpha (PGF 2 alpha) analogs to mobilize intracellular Ca2+[Ca2+]iand to compete for [3H]PGF 2 alpha binding to prostaglandin F 2 alpha receptors (FP) was evaluated. Tritium 130-132 placental growth factor Rattus norvegicus 52-55 12490597-1 2003 The ability of a number of prostaglandin F 2 alpha (PGF 2 alpha) analogs to mobilize intracellular Ca2+[Ca2+]iand to compete for [3H]PGF 2 alpha binding to prostaglandin F 2 alpha receptors (FP) was evaluated. Tritium 130-132 placental growth factor Rattus norvegicus 133-136 12490613-0 2003 Sigma1 receptor agonist-mediated regulation of N-methyl-D-aspartate-stimulated [3H]dopamine release is dependent upon protein kinase C. Tritium 80-82 sigma non-opioid intracellular receptor 1 Rattus norvegicus 0-15 11263669-8 2001 RESULTS: BMP-7 inhibited both serum-stimulated and growth factor-induced (PDGF-BB and TGF-beta1) SMC growth, as measured by 3H-thymidine uptake and cell number, in primary human aortic smooth muscle (HASM) cell cultures. Tritium 124-126 bone morphogenetic protein 7 Homo sapiens 9-14 10913689-5 2001 Increases in [3H]nisoxetine binding were found in the cingulate, frontal, parietal, agranular insular, entorhinal and perirhinal cortices as well as in the hippocampal divisions CA1, CA3, dentate gyrus and subiculum, and in nucleus raphe dorsalis of trimipramine-treated animals compared to the control rats. Tritium 14-16 carbonic anhydrase 3 Rattus norvegicus 183-186 11715432-4 1999 Adm, CNP, CGRP, SST, and PTHrP had no effect on basal 3H-TdR incorporation, but they could inhibit Hcy-induced 3H-TdR incorporation in dose-dependent manner. Tritium 111-113 parathyroid hormone-related protein Oryctolagus cuniculus 25-30 12490613-0 2003 Sigma1 receptor agonist-mediated regulation of N-methyl-D-aspartate-stimulated [3H]dopamine release is dependent upon protein kinase C. Tritium 80-82 protein kinase C, gamma Rattus norvegicus 118-134 12490613-3 2003 We tested several inhibitors of PKC isozymes, as well as a phospholipase C inhibitor for their effects on sigma1 receptor agonist-mediated regulation of [3H]dopamine release. Tritium 154-156 protein kinase C, gamma Rattus norvegicus 32-35 12490613-3 2003 We tested several inhibitors of PKC isozymes, as well as a phospholipase C inhibitor for their effects on sigma1 receptor agonist-mediated regulation of [3H]dopamine release. Tritium 154-156 sigma non-opioid intracellular receptor 1 Rattus norvegicus 106-121 12490613-6 2003 We concluded that an intact PKC system was required for sigma1 agonist-mediated regulation of NMDA-stimulated [3H]dopamine release from rat striatal slices. Tritium 111-113 protein kinase C, gamma Rattus norvegicus 28-31 12397753-6 2002 The ID50 of hepatoma-derived growth factor antisense phosphorothioate oligonucleotides for HuH-7 cells, in which hepatoma-derived growth factor expression was abundant, was 3 microM by the assay of cell proliferation and [3H]-thymidine incorporation. Tritium 222-224 MIR7-3 host gene Homo sapiens 91-96 11680691-8 2001 The dissociation of [3H]GDP from RhoA was stimulated dose-dependently by Sf9 cell lysates containing the CDEP peptide. Tritium 21-23 FERM, RhoGEF (Arhgef) and pleckstrin domain protein 1 (chondrocyte-derived) Mus musculus 105-109 11179597-4 2001 Rat CRFBP was pharmacologically characterized employing a competition binding assay with tritium-labeled rat urocortin. Tritium 89-96 corticotropin releasing hormone binding protein Rattus norvegicus 4-9 11147830-3 2000 We show that the human hepatoma line SK-Hep-1 has cytosolic Ah receptor detectable by specific binding of [3H]TCDD. Tritium 107-109 DNL-type zinc finger Homo sapiens 40-45 10470140-4 1999 Exposure of both cell lines to 20 ppm CLA for 2-6 days produced a reduction (83-91%) in 3H-leucine incorporation into protein while 3H-uridine and 3H-thymidine incorporation into RNA and DNA were reduced by 49-91% and 86-98%, respectively, compared with untreated control cells. Tritium 88-90 selectin P ligand Homo sapiens 38-41 11099697-4 2000 [3H]Methotrexate uptake was markedly stimulated in the rOAT1 cRNA-injected oocytes, and this uptake was inhibited by probenecid and various NSAIDs, whereas the influence of salicylate was less. Tritium 1-3 solute carrier family 22 member 6 Rattus norvegicus 55-60 12590956-9 2002 Binding studies using [3H] Roxifiban, a high affinity GP IIb/IIIa antagonist, indicate that occupancy of >95% of GP IIb/IIIa sites is required to inhibit clot retraction. Tritium 23-25 integrin subunit alpha 2b Homo sapiens 54-60 10470140-4 1999 Exposure of both cell lines to 20 ppm CLA for 2-6 days produced a reduction (83-91%) in 3H-leucine incorporation into protein while 3H-uridine and 3H-thymidine incorporation into RNA and DNA were reduced by 49-91% and 86-98%, respectively, compared with untreated control cells. Tritium 132-134 selectin P ligand Homo sapiens 38-41 10386791-0 1999 Overexpression, purification and photoaffinity labeling with a 3H-analogue of norfloxacin, of the GyrA and GyrB subunits of the DNA gyrase. Tritium 63-65 DNA topoisomerase II alpha Homo sapiens 128-138 12081328-7 2002 Binding affinity was measured by competition with 3H-PK11195, a high-affinity ligand of PBR, for binding to rat kidney mitochondria (RKM) or intact Chinese hamster ovary (CHO) cells. Tritium 50-52 translocator protein Rattus norvegicus 88-91 12081328-8 2002 To assess the binding of the Pc directly, we synthesized 14C-labeled Pc 4 and found that whereas Pc 4 was a competitive inhibitor of 3H-PK11195 binding to the PBR, PK11195 did not inhibit the binding of 14C-Pc 4 to RKM. Tritium 133-135 translocator protein Rattus norvegicus 159-162 11076596-7 2000 Contrasting behavior of the D-ring methyl-substituted 9 and the C-11 methoxy-substituted 10 dimers is remarkable in that unlike 9 which is readily cleaved to produce the monomeric arenium ion 3H(+)(), 10 is diprotonated at the two C-12 sites and at C-12/C-14 in each unit. Tritium 192-194 RNA polymerase III subunit K Homo sapiens 64-68 11033075-7 2000 When verapamil or cyclosporin A, potent modulators of P-gp, was added to the apical medium together with unlabeled VLB, enhanced basolateral-to-apical transport of [3H]VLB was disappeared. Tritium 165-167 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 54-58 10340592-6 1999 The [3H]folic acid and FBP peaks coincided, indicating that the RIA is specific for FBP. Tritium 5-8 folate receptor alpha Homo sapiens 84-87 10975816-3 2000 Skin- and blood-derived nickel-specific CD8+ T cytotoxic 1 (Tc1) and Tc2 clones as well as CD4+ Th1 and Th2 expressed the cutaneous lymphocyte-associated Ag and exhibited strong MHC-restricted cytotoxicity against nickel-coupled B lymphoblasts, as detected by the [3H]TdR release assay. Tritium 265-267 CD8a molecule Homo sapiens 40-43 11888550-12 2002 Finally, in line with species differences, the affinities of several ligands including CP93,129, RU24,969, (-)-pindolol and (-)-propanolol in rat 5-HT(1B) sites were markedly different to guinea pig 5-HT(1B) sites labelled with [3H]GR125,743. Tritium 229-231 5-hydroxytryptamine receptor 1B Rattus norvegicus 146-153 11888550-13 2002 In conclusion, [3H]GR125,743 is an appropriate tool for the radiolabelling of native, rat 5-HT(1B) receptors and permitted determination of the affinities of an extensive series of ligands at these sites. Tritium 16-18 5-hydroxytryptamine receptor 1B Rattus norvegicus 90-97 11956958-2 2002 The nicotinic receptor (nAChR) binding sites were measured by (-)-[3H]nicotine and [3H]epibatidine, and the muscarinic receptor (mAChR) binding sites by [3H]pirenzepine and [3H]AFDX 384. Tritium 67-69 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 24-29 10231436-8 1999 Pretreatment of MCs with aristolochic acid, a PLA2 inhibitor, concordantly reduced IL-1-regulated [3H]AA release and JNK1/SAPK activity, suggesting that cytosolic AA in part mediates IL-1-induced JNK1/SAPK activation. Tritium 99-101 interleukin 1 alpha Homo sapiens 83-87 11956958-2 2002 The nicotinic receptor (nAChR) binding sites were measured by (-)-[3H]nicotine and [3H]epibatidine, and the muscarinic receptor (mAChR) binding sites by [3H]pirenzepine and [3H]AFDX 384. Tritium 84-86 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 24-29 10936544-3 2000 We confirmed the previous results by showing that HD11 macrophages pre-treated for 24h with recombinant chicken IFN-gamma (either produced in E. coli or by transfected COS cells), at doses ranging from 1000 to 10U/ml, drastically inhibited E. tenella replication as measured by [3H] uracil uptake after a further 70h of culture, as when treated with REV supernatant. Tritium 279-281 interferon Gallus gallus 112-121 10898749-4 2000 The CGRP receptor antagonist, CGRP-(8-37), prevented the increase in (3)H-labeled corpus mucin. Tritium 69-73 solute carrier family 13 member 2 Rattus norvegicus 89-94 10216201-5 1999 Phospholipase A2 (PLA2, 2 U/ml), an enzyme that releases AA from the sn-2 position of phospholipids, mimicked the facilitatory effect of AA on the evoked tritium release (86+/-14% facilitation), an effect prevented by BSA (1%). Tritium 154-161 phospholipase A2 group IB Rattus norvegicus 18-22 10216201-6 1999 The PLA2 activator, melittin (1 microM), enhanced the evoked tritium release by 98+/-11%, an effect prevented by the PLA2 inhibitor, arachidonyl trifluromethylketone (AACOCF3, 20 microM), and by BSA (1%). Tritium 61-68 phospholipase A2 group IB Rattus norvegicus 4-8 11939716-4 2002 METHODS: Culture of rat buccal mucosa in the presence of ethanol and [3H]-labeled proline and palmitate revealed substantial decrease in MBP synthesis and the release of MBPto the medium. Tritium 70-72 myelin basic protein Rattus norvegicus 137-140 11820773-3 2002 After incubation for 6 or 24 h, 3H-cholesterol efflux to free apoA-I (10 microg/ml) was significantly higher with macrophages derived from C3H mice compared to C57BL/6 mice. Tritium 32-34 apolipoprotein A-I Mus musculus 62-68 11984695-10 2002 CNP or 8-bromo cGMP reduced [3H] thymidine uptake and slightly increased the message for collagen type X, which is a marker of hypertrophic chondrocytes. Tritium 29-31 natriuretic peptide type C Mus musculus 0-3 10867813-9 2000 [3H]-thymidine uptake by SSc fibroblasts expressing high amounts of alpha 1(I) procollagen mRNA was significantly lower than by those expressing low amounts (P < 0.05). Tritium 1-3 collagen type I alpha 1 chain Homo sapiens 68-90 10781929-4 2000 Pretreatment with PTX resulted in dose-responsive decreases in insulin-stimulated [3H]2-deoxyglucose (DOG) uptake, and unchanged TPA-stimulated [3H]2-DOG uptake, without affecting basal [3H]2-DOG uptake. Tritium 83-85 insulin Canis lupus familiaris 63-70 10737611-2 2000 In this study, substrate recognition was studied via the inhibition of binding of [3H]WIN 35,428 [2beta-carbomethoxy-3beta-(4-fluorophenyl)[3H]tropane], a cocaine analogue, to the human dopamine transporter in human embryonic kidney 293 cells. Tritium 83-85 solute carrier family 6 member 3 Homo sapiens 186-206 10216201-6 1999 The PLA2 activator, melittin (1 microM), enhanced the evoked tritium release by 98+/-11%, an effect prevented by the PLA2 inhibitor, arachidonyl trifluromethylketone (AACOCF3, 20 microM), and by BSA (1%). Tritium 61-68 phospholipase A2 group IB Rattus norvegicus 117-121 10771005-8 2000 The majority of beta2-independent [3H]epibatidine binding was located in small, scattered brain nuclei, suggesting that individual nuclei may prove suitable for identification of novel, native nicotinic acetylcholine receptors. Tritium 35-37 hemoglobin, beta adult minor chain Mus musculus 16-21 11739621-4 2001 [3H]Dopamine (100 nM) accumulation into DAT-expressing oocytes increased significantly in response to ethanol (10 min; 10-100 mM). Tritium 1-3 solute carrier family 6 (neurotransmitter transporter), member 3 S homeolog Xenopus laevis 40-43 11739621-7 2001 Cell surface DAT binding ([3H]WIN 35,428; 4 nM) also increased as a function of ethanol exposure time. Tritium 27-29 solute carrier family 6 member 3 Homo sapiens 13-16 10069986-2 1999 We have studied the effects of several inhibitors of NADPH oxidase on the normoxic and hypoxia-induced release of [3H]catecholamines (CA) in an in vitro preparation of intact CB of the rat and rabbit whose CA deposits have been labeled by prior incubation with the natural precursor [3H]tyrosine. Tritium 114-118 NADPH oxidase 1 Oryctolagus cuniculus 53-66 11714843-6 2001 These cells incubated without or with LPL in medium containing 125I/[3H]cholesteryl oleyl ether- labeled HDL3; tetrahydrolipstatin inhibited the catalytic activity of LPL. Tritium 69-71 high density lipoprotein (HDL) level 3 Mus musculus 105-109 11714843-6 2001 These cells incubated without or with LPL in medium containing 125I/[3H]cholesteryl oleyl ether- labeled HDL3; tetrahydrolipstatin inhibited the catalytic activity of LPL. Tritium 69-71 lipoprotein lipase Mus musculus 167-170 11714843-7 2001 In BHK and in Y1-BS1 cells without or with SR-BI expression, apparent HDL3 selective CE uptake ([3H]CEt - 125I) was detectable. Tritium 97-99 high density lipoprotein (HDL) level 3 Mus musculus 70-74 11500505-6 2001 We found that only nonconservative substitutions (Ala and Cys) of Trp1254 eliminated [3H]E217betaG transport by MRP2, whereas more conservative substitutions (Phe and Tyr) had no effect. Tritium 86-88 ATP binding cassette subfamily C member 2 Homo sapiens 112-116 11500505-9 2001 Finally, we found that sulfinpyrazone stimulated [3H]E217betaG transport by wild-type MRP2 4-fold, whereas transport by the Trp1254 substituted mutants was enhanced 6-10-fold. Tritium 50-52 ATP binding cassette subfamily C member 2 Homo sapiens 86-90 10719063-4 2000 Globally, the labeling was the highest with (-)[3H]GK11. Tritium 48-50 kallikrein 1-related peptidase C6 Rattus norvegicus 51-55 10719063-7 2000 In all regions, (-)[3H]GK11 exhibited the highest affinity in the nanomolar range. Tritium 20-22 kallikrein 1-related peptidase C6 Rattus norvegicus 23-27 10728698-10 2000 Functional assays using [3H]thymidine incorporation indicated that granulin may be a glial mitogen, as addition of synthetic granulin peptide to primary rat astrocytes and three different early-passage human glioblastoma cultures increased cell proliferation in vitro, whereas increasing concentrations of granulin antibody inhibited cell growth in a dose-dependent manner. Tritium 25-27 granulin precursor Rattus norvegicus 67-75 10646533-8 2000 A human NA transporter-transfected CATH.a cell line was generated (termed RUNT), and a study of the [3H]NA uptake revealed that the RUNT cells displayed significant uptake that could be blocked by cocaine (10 microM). Tritium 101-103 cathepsin H Mus musculus 35-39 10069986-3 1999 It was found that diphenyleneiodonium (DPI; 0.2-25 microM), an inhibitor of NADPH oxidase, caused a dose-dependent release of [3H]CA from normoxic CB chemoreceptor cells. Tritium 127-129 NADPH oxidase 1 Oryctolagus cuniculus 76-89 10051484-6 1999 Mrp2-mediated [3H]leukotriene excretion into bile during the initial 45 minutes was reduced to 44% of control when [3H]LTC4 was injected 15 minutes after phalloidin treatment. Tritium 15-17 ATP binding cassette subfamily B member 4 Rattus norvegicus 0-4 10666504-5 2000 The adrenomedullin-induced decrease in [3H]-thymidine incorporation and increase in apoptosis were reversed by okadaic acid at the concentration that selectively inhibits PP2A. Tritium 40-42 adrenomedullin Rattus norvegicus 4-18 10954043-5 2000 The EGF-induced increase in [3H]-labeled mucin in the full-thickness mucosa was not suppressed by either NG-nitro-L-arginine (10(-5) M) or 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (10(-5) M). Tritium 29-31 solute carrier family 13 member 2 Rattus norvegicus 41-46 11543661-5 2001 Recombinant APT1 was purified from Escherichia coli, and depalmitoylation of [3H]palmitate-labeled glycoproteins present in virus particles was measured by SDS-PAGE, fluorography, and scanning densitometry. Tritium 78-80 lysophospholipase 1 Homo sapiens 12-16 10051484-6 1999 Mrp2-mediated [3H]leukotriene excretion into bile during the initial 45 minutes was reduced to 44% of control when [3H]LTC4 was injected 15 minutes after phalloidin treatment. Tritium 116-118 ATP binding cassette subfamily B member 4 Rattus norvegicus 0-4 11524244-9 2001 TGF-alpha and TGF-beta stimulated [3H]thymidine incorporation by 2.2- to 3.1-fold and 1.7- to 3.4-fold respectively (P<0.005). Tritium 35-37 transforming growth factor alpha Rattus norvegicus 0-9 10082496-2 1999 The potentiation and the desensitization and resensitization of B2 receptor were assessed by measuring [3H]arachidonic acid release and [Ca2+]i mobilization in Chinese hamster ovary cells transfected to express human ACE and B2 receptor, or in endothelial cells with constitutively expressed ACE and receptor. Tritium 104-106 bradykinin receptor B2 Homo sapiens 64-75 11562118-4 2001 AdBMP-7 modification of bovine chondrocytes induced expression of BMP-7 mRNA and bioactive protein, resulting in an increase in incorporation of 35SO4- into proteoglycan, 3H-proline uptake into protein, and the expression of the cartilage-specific matrix genes, aggrecan and type II collagen. Tritium 171-173 bone morphogenetic protein 7 Bos taurus 2-7 11039605-2 1999 When cell growth was determined by [3H]thymidine incorporation, it was significantly stimulated by VEGF stimulation. Tritium 36-38 vascular endothelial growth factor A Bos taurus 99-103 10598792-3 1999 The specific binding of [3H]prazosin to membranes of U373 MG cells (Bmax 32+/-3 fmol mg(-1) protein, Kd 0.27+/-0.03 nM) was inhibited in a monophasic manner by alpha1-antagonists that have different affinities for alpha1A-, alpha1B- and alpha1D-adrenoceptors. Tritium 25-27 calcium voltage-gated channel subunit alpha1 A Homo sapiens 214-221 10536271-2 1999 Saturable specific binding of [3H]spermine to synaptosomal membranes from plexiform layers of retina (P1 and P2) has been characterized, and found to concentrate in the inner plexiform layer compared to the outer plexiform layer (Bmax=9.3 and 37 pmol/mg protein for P1 and P2, respectively). Tritium 31-33 perforin 1 Gallus gallus 102-111 10536271-2 1999 Saturable specific binding of [3H]spermine to synaptosomal membranes from plexiform layers of retina (P1 and P2) has been characterized, and found to concentrate in the inner plexiform layer compared to the outer plexiform layer (Bmax=9.3 and 37 pmol/mg protein for P1 and P2, respectively). Tritium 31-33 perforin 1 Gallus gallus 266-275 10536271-3 1999 Kinetics of specific [3H]spermine binding yield a sigmoidal saturation curve, indicating positive cooperativity (nH: 2.4 and 3.2 for P1 and P2, respectively) with high affinity: Kapp=61 and 67 nM for P1 and P2. Tritium 22-24 perforin 1 Gallus gallus 133-142 10536271-3 1999 Kinetics of specific [3H]spermine binding yield a sigmoidal saturation curve, indicating positive cooperativity (nH: 2.4 and 3.2 for P1 and P2, respectively) with high affinity: Kapp=61 and 67 nM for P1 and P2. Tritium 22-24 perforin 1 Gallus gallus 200-209 11487728-5 2001 The A-11 clone yielded [3H]-adenosine flux ratios of 400 to 500 after injection of cRNA in oocytes. Tritium 24-26 selectin L Rattus norvegicus 4-8 11384779-3 2001 However, studies on clonal lines of IEC-6 cells stably transfected with PTHrP cDNA and overexpressing PTHrP showed that increased PTHrP production enhanced cell growth and 3H-thymidine incorporation in high, but not low, serum. Tritium 172-174 parathyroid hormone-like hormone Rattus norvegicus 72-77 11384779-3 2001 However, studies on clonal lines of IEC-6 cells stably transfected with PTHrP cDNA and overexpressing PTHrP showed that increased PTHrP production enhanced cell growth and 3H-thymidine incorporation in high, but not low, serum. Tritium 172-174 parathyroid hormone-like hormone Rattus norvegicus 102-107 10514037-1 1999 Hepatic AhR binding affinity for [3H]-2,3,7,8-tetrachlorodibenzo-p-dioxin ([3H]TCDD) was compared between two species widely used as laboratory animals: beagle dog and cynomolgus monkey (Macaca fascicularis). Tritium 34-36 aryl hydrocarbon receptor Canis lupus familiaris 8-11 10452108-4 1999 RESULTS: VATP 50-200 mg.L-1 and VAP-B 12.5, 25, and 50 mg.L-1 showed most marked proliferation-promoting activity for rabbit costed chondrocytes and increased incorporation of [3H]TdR from (73 +/- 9) Bq (control group) to (272 +/- 55), (327 +/- 38), and (415 +/- 32) Bq, respectively (P < 0.01). Tritium 177-179 vesicle-associated membrane protein-associated protein B/C Oryctolagus cuniculus 32-37 10514037-1 1999 Hepatic AhR binding affinity for [3H]-2,3,7,8-tetrachlorodibenzo-p-dioxin ([3H]TCDD) was compared between two species widely used as laboratory animals: beagle dog and cynomolgus monkey (Macaca fascicularis). Tritium 33-36 aryl hydrocarbon receptor Canis lupus familiaris 8-11 10514037-3 1999 After incubation with [3H]TCDD, dextran-coat charcoal treatment (10 mg/ml) revealed that dog and monkey possess an AhR with a low binding affinity for [3H]TCDD. Tritium 23-25 aryl hydrocarbon receptor Canis lupus familiaris 115-118 10514037-3 1999 After incubation with [3H]TCDD, dextran-coat charcoal treatment (10 mg/ml) revealed that dog and monkey possess an AhR with a low binding affinity for [3H]TCDD. Tritium 152-154 aryl hydrocarbon receptor Canis lupus familiaris 115-118 10510460-4 1999 E-2 displaced [3H]-DPN binding in CHOmu and SH-SY5Y cells with pKi values of 7.82+/-0.11 and 8.43+/-0.13 respectively. Tritium 15-17 cystatin 12, pseudogene Homo sapiens 0-3 11384779-3 2001 However, studies on clonal lines of IEC-6 cells stably transfected with PTHrP cDNA and overexpressing PTHrP showed that increased PTHrP production enhanced cell growth and 3H-thymidine incorporation in high, but not low, serum. Tritium 172-174 parathyroid hormone-like hormone Rattus norvegicus 102-107 11384779-5 2001 In three clonal IEC-6 lines transfected with PTHrP cDNA bearing a mutated NLS, the ability of PTHrP to stimulate 3H-thymidine incorporation and cell growth was lost. Tritium 113-115 parathyroid hormone-like hormone Rattus norvegicus 45-50 11384779-5 2001 In three clonal IEC-6 lines transfected with PTHrP cDNA bearing a mutated NLS, the ability of PTHrP to stimulate 3H-thymidine incorporation and cell growth was lost. Tritium 113-115 parathyroid hormone-like hormone Rattus norvegicus 94-99 9988695-5 1999 Whereas [3H]globotriaosylceramide ([3H]Gb3) comprised 1.9% of the total [3H]SLs and [3H]GSLs synthesized in control cells, it comprised 16. Tritium 9-11 alpha 1,4-galactosyltransferase (P blood group) Homo sapiens 39-42 11353141-7 2001 LTP as well as [3H]MK-801 binding were significantly reduced in the cortex following PCB exposure, while [3H]MK-801 binding in the hippocampus was not affected. Tritium 16-18 pyruvate carboxylase Rattus norvegicus 85-88 11353141-10 2001 Taking the two experiments together, PCB exposure significantly reduced LTP, as well as [3H]MK-801 binding, in the cortex and had no effect in the hippocampus. Tritium 89-91 pyruvate carboxylase Rattus norvegicus 37-40 11356255-3 2001 Stimulation of BAEC with recombinant bovine tumor necrosis factor alpha (rbTNF-alpha) resulted in protein expression of VCAM-1 on less than 5% of all cultured BAECs at 1h post-stimulation, followed by a significant increase at 3h that was maintained until 48h when the proportion of VCAM-1 positive (+) cells decreased significantly, but not to baseline proportions. Tritium 227-229 vascular cell adhesion molecule 1 Bos taurus 120-126 10497908-5 1999 Decynium22 and corticosterone, known inhibitors of rat Organic Cation Transporter 1 (rOCT1), markedly reduced [3H]MPP+ efflux. Tritium 111-113 solute carrier family 22 member 1 Rattus norvegicus 55-83 10497908-5 1999 Decynium22 and corticosterone, known inhibitors of rat Organic Cation Transporter 1 (rOCT1), markedly reduced [3H]MPP+ efflux. Tritium 111-113 solute carrier family 22 member 1 Rattus norvegicus 85-90 10497908-7 1999 In conclusion, [3H]MPP+ efflux from primary cultured rat hepatocytes appears to be mediated by rOCT1, a polyspecific organic cation transporter. Tritium 16-18 solute carrier family 22 member 1 Rattus norvegicus 95-100 9988695-5 1999 Whereas [3H]globotriaosylceramide ([3H]Gb3) comprised 1.9% of the total [3H]SLs and [3H]GSLs synthesized in control cells, it comprised 16. Tritium 36-38 alpha 1,4-galactosyltransferase (P blood group) Homo sapiens 39-42 11240028-4 2001 The efflux of [3H]mouse IgG(2a) from brain to blood is competitively inhibited by intracerebral injection of unlabeled mouse IgG molecules, but is not inhibited by intracerebral injection of comparable doses of unlabeled rat albumin. Tritium 15-17 gamma-2a immunoglobulin heavy chain Rattus norvegicus 24-30 9988695-5 1999 Whereas [3H]globotriaosylceramide ([3H]Gb3) comprised 1.9% of the total [3H]SLs and [3H]GSLs synthesized in control cells, it comprised 16. Tritium 36-38 alpha 1,4-galactosyltransferase (P blood group) Homo sapiens 39-42 10497873-7 1999 The reduction in PKR activity and subsequent deregulation of eIF-2alpha phosphorylation was related to appearance of tumor-like cellular morphology and increased cell density as shown by cell counts and [3H]-thymidine uptake. Tritium 204-206 eukaryotic translation initiation factor 2-alpha kinase 2 Mus musculus 17-20 10497873-7 1999 The reduction in PKR activity and subsequent deregulation of eIF-2alpha phosphorylation was related to appearance of tumor-like cellular morphology and increased cell density as shown by cell counts and [3H]-thymidine uptake. Tritium 204-206 eukaryotic translation initiation factor 2-alpha kinase 2 Mus musculus 61-71 10391897-1 1999 Using a novel antibody directed against the alpha4 subunit of gamma-aminobutyric acidA (GABAA) receptors, 5% of all [3H]muscimol but only about 2% of all [3H]Ro15-4513 binding sites present in brain membrane extracts could be precipitated. Tritium 117-119 immunoglobulin binding protein 1 Homo sapiens 44-50 10391897-1 1999 Using a novel antibody directed against the alpha4 subunit of gamma-aminobutyric acidA (GABAA) receptors, 5% of all [3H]muscimol but only about 2% of all [3H]Ro15-4513 binding sites present in brain membrane extracts could be precipitated. Tritium 155-157 immunoglobulin binding protein 1 Homo sapiens 44-50 10391897-2 1999 This indicated that part of the alpha4 receptors containing [3H]muscimol binding sites did not contain [3H]Ro15-4513 binding sites. Tritium 61-63 immunoglobulin binding protein 1 Homo sapiens 32-38 10403380-3 1999 Transfection of cPLA2 into sPLA2-IIA-expressing cells produced a synergistic increase in IL-1-dependent [3H]AA release and subsequent prostaglandin production. Tritium 105-107 phospholipase A2 group IVA Homo sapiens 16-21 11741514-8 2001 MDR-reversing agents and antibody of P-gp, C219, level-dependently inhibited the uptake of [3H]Glu by luminal membrane vesicles of BCEC. Tritium 92-94 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 37-41 11159051-10 2001 Adrenomedullin dose-dependently inhibited platelet-derived growth factor-stimulated [3H]thymidine incorporation and endothelin release in ADM- cells but did not affect [3H]thymidine uptake in ADM+ isolates. Tritium 85-87 adrenomedullin Homo sapiens 0-14 11160629-6 2001 The affinity of DA for the DAT, as measured by [3H]DA uptake experiments, was higher in both the cryopreserved human caudate and freshly prepared rat striatum than in HEK-hDAT cells. Tritium 48-50 solute carrier family 6 member 3 Homo sapiens 27-30 9988695-5 1999 Whereas [3H]globotriaosylceramide ([3H]Gb3) comprised 1.9% of the total [3H]SLs and [3H]GSLs synthesized in control cells, it comprised 16. Tritium 36-38 alpha 1,4-galactosyltransferase (P blood group) Homo sapiens 39-42 9950961-6 1999 When expressed transiently in vitro, hPAHT catalyzed time-dependent and saturable [3H]PAH uptake (Km of approximately 5 microM). Tritium 83-85 solute carrier family 22 member 6 Homo sapiens 37-42 11166699-5 2001 Basal forebrain cholinergic hypofunction resulted in reduced catalytic and substrate binding activity of nNOS in a number of hippocampal and neocortical subregions 7 days after lesion as revealed by NADPH-diaphorase enzyme histochemistry and quantitative autoradiography of [3H]L-N(G)-nitro-arginine binding, respectively. Tritium 275-277 nitric oxide synthase 1 Rattus norvegicus 105-109 11920258-5 2001 The effect of IFN-alpha in terms of proliferation, induction of apoptosis, changes in cell cycle inhibitor proteins, and differentiation was assessed by [3H]thymidine incorporation, Annexin V and Western blot analysis. Tritium 154-156 interferon alpha Mus musculus 14-23 10435785-9 1999 In contrast, neurotensin-immunoreactive cells in the dorsal and median raphe were distributed among [3H]5-HT radiolabelled neurons but they were never radiolabelled. Tritium 101-103 neurotensin Rattus norvegicus 13-24 10349847-3 1999 Ligand affinities for the active and inactive state of the CB1 receptor were determined by competition with [3H]CP55940 and [3H]SR141716A in the presence of 5"-guanylylimidodiphosphate, respectively. Tritium 109-111 cannabinoid receptor 1 Rattus norvegicus 59-62 10349847-3 1999 Ligand affinities for the active and inactive state of the CB1 receptor were determined by competition with [3H]CP55940 and [3H]SR141716A in the presence of 5"-guanylylimidodiphosphate, respectively. Tritium 125-127 cannabinoid receptor 1 Rattus norvegicus 59-62 9950616-2 1999 METHODS: [3H]all-trans retinol (ROL) was delivered to the basal surface of the cultured RPE by serum retinol-binding protein (RBP). Tritium 10-12 retinol binding protein 4 Bos taurus 126-129 10431757-2 1999 Furthermore, the affinity of the drugs for cannabinoid CB1 receptors was determined in rat brain cortex membranes using [3H]SR 141716. Tritium 121-123 cannabinoid receptor 1 Rattus norvegicus 55-58 11182247-8 2001 The pattern of changes in neurotensin/neuromedin N messenger RNA caused by methamphetamine and cocaine after 3h was even more distinct. Tritium 109-111 neurotensin Rattus norvegicus 26-50 11182247-12 2001 The only significant effect was an increase in neurotensin/neuromedin N messenger RNA in the core region 3h after methamphetamine administration. Tritium 105-107 neurotensin Rattus norvegicus 47-71 10431757-4 1999 The CB1 receptor antagonist SR 141716 increased the NMDA (1000 microM)-stimulated tritium overflow but did not affect tritium overflow stimulated by NMDA 100 microM or kainate 1000 microM. Tritium 82-89 cannabinoid receptor 1 Rattus norvegicus 4-7 9878841-4 1999 These sizable genotypic differences in analgesic potency were accompanied by HA/LA and HAR/LAR differences in whole-brain homogenate [3H]DPDPE and/or [3H]DELT, but paradoxically not [3H]DAMGO, binding. Tritium 134-136 low antibody response Mus musculus 91-94 9878841-4 1999 These sizable genotypic differences in analgesic potency were accompanied by HA/LA and HAR/LAR differences in whole-brain homogenate [3H]DPDPE and/or [3H]DELT, but paradoxically not [3H]DAMGO, binding. Tritium 151-153 low antibody response Mus musculus 91-94 11108289-13 2000 Likewise, NGF stimulated [3H]thymidine incorporation into trkA-containing cells, but not into cells that had lost the receptor in culture. Tritium 26-28 neurotrophic receptor tyrosine kinase 1 Rattus norvegicus 58-62 10075154-4 1999 Fluoride at micromolar concentrations significantly and dose-dependently stimulated [3H]thymidine incorporation into DNA in DP-1, DP-2 and TE-85 cells, with optimal effects around 50 microM, by 127 +/- 7%, 124 +/- 0.6% and 152 +/- 13.4%, respectively. Tritium 85-87 transcription factor Dp-2 Homo sapiens 130-134 10369486-2 1999 Furthermore, the affinities of ORL1 ligands were determined using [3H]-nociceptin binding. Tritium 67-69 opioid receptor-like 1 Mus musculus 31-35 10188142-3 1999 Competition studies employing some selective agonists for NK1, NK2 and NK3 receptors have demonstrated that only the NK1 selective agonist, [Sar9, Met(O2)11]-SP, was a competitor for [3H]SP binding. Tritium 184-186 tachykinin receptor 1 Homo sapiens 117-120 10216115-4 1999 Mucin secretion by confluent monolayers of DGBE cells was quantified by measuring the secretion of [3H]-N-acetyl-D-glucosamine-labeled glycoproteins. Tritium 100-102 mucin Canis lupus familiaris 0-5 11194707-2 2000 In this study we describe growth promoting effects (determined by 3H-TdR incorporation assay) of TBP (5 microg/l, i.e. 10% w/v plasma equivalent concentration) for 10 non-functioning pituitary tumors. Tritium 66-68 TATA-box binding protein Homo sapiens 97-100 11194707-4 2000 Hence, similar to its effects on hormonal activity of the pituitary tumors, TBP stimulated the growth of the tumors which did not express high spontaneous 3H-TdR intensity, but did not stimulate the cells with high capacity of spontaneous 3H-TdR incorporation. Tritium 155-157 TATA-box binding protein Homo sapiens 76-79 10188142-5 1999 These data show that in the skin [3H]SP binds to a single population of substance P high-affinity sites, which represent NK1-type receptors. Tritium 34-36 tachykinin receptor 1 Homo sapiens 121-124 9886269-9 1999 In experiments with a bacterially expressed PAI-2 fusion protein, [3H]thymidine incorporation by keratinocytes was significantly suppressed, confirming an anti-proliferative effect of PAI-2. Tritium 67-69 serpin family B member 2 Homo sapiens 44-49 11065167-4 2000 In saturation experiments, [3H]-JTH-601 showed subtype selectivity: high affinity to alpha1a-AR (pKd; 9.88+/-0.09), lower affinity to alpha1b-AR (pKd; 8.96+/-0.17) and no specific binding at concentrations up to 3000 pM to alpha1d-AR. Tritium 28-30 protein kinase D1 Homo sapiens 97-100 11065167-4 2000 In saturation experiments, [3H]-JTH-601 showed subtype selectivity: high affinity to alpha1a-AR (pKd; 9.88+/-0.09), lower affinity to alpha1b-AR (pKd; 8.96+/-0.17) and no specific binding at concentrations up to 3000 pM to alpha1d-AR. Tritium 28-30 adrenoceptor alpha 1B Homo sapiens 134-144 11065167-4 2000 In saturation experiments, [3H]-JTH-601 showed subtype selectivity: high affinity to alpha1a-AR (pKd; 9.88+/-0.09), lower affinity to alpha1b-AR (pKd; 8.96+/-0.17) and no specific binding at concentrations up to 3000 pM to alpha1d-AR. Tritium 28-30 protein kinase D1 Homo sapiens 146-149 11065167-6 2000 [3H]-JTH-601 also bound to human prostate membranes in monophasic manner with high affinity constant (pKd; 9.89+/-0.12, Bmax=123.6+/-16 fmol/mg protein). Tritium 1-3 protein kinase D1 Homo sapiens 102-105 11029639-8 2000 In support of this proposal, in nonhypoxic PC12 cells constitutively coexpressing HIF-1alpha, ARNT1 and ARNT2, downregulation of either HIF-1alpha or ARNT2, obtained with selective antisense nucleotides, resulted in inhibition of [3H]thymidine incorporation. Tritium 231-233 hypoxia inducible factor 1 subunit alpha Rattus norvegicus 82-92 11029639-8 2000 In support of this proposal, in nonhypoxic PC12 cells constitutively coexpressing HIF-1alpha, ARNT1 and ARNT2, downregulation of either HIF-1alpha or ARNT2, obtained with selective antisense nucleotides, resulted in inhibition of [3H]thymidine incorporation. Tritium 231-233 hypoxia inducible factor 1 subunit alpha Rattus norvegicus 136-146 11044655-3 2000 Binding capacity for the PBR ligand [3H]-Ro5-4864 was strongly related to the phase of the cell cycle with a positive correlation (r=0.98) with a high percentage of cells in S phase. Tritium 37-39 translocator protein Homo sapiens 25-28 10200422-2 1999 The release of [3H]-noradrenaline ([3H]-NA) in response to nicotinic acetylcholine receptor (nAChR) agonists was compared with agonist-induced currents in cultured rat superior cervical ganglion (SCG) neurones. Tritium 16-18 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 59-91 10200422-4 1999 [3H]-NA release in response to high concentrations of nicotinic agonists was reduced, but not fully inhibited, by the presence of either tetrodotoxin (TTX) or Cd2+ to block voltage-gated Na+ or Ca2+ channels, respectively. Tritium 1-3 Cd2 molecule Rattus norvegicus 159-162 10200422-8 1999 [3H]-NA release in response to low concentrations of nAChR agonists was fully inhibited by the presence of either TTX or Cd2+ (named TTX- or Cd2+-sensitive release). Tritium 1-3 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 53-58 10200422-8 1999 [3H]-NA release in response to low concentrations of nAChR agonists was fully inhibited by the presence of either TTX or Cd2+ (named TTX- or Cd2+-sensitive release). Tritium 1-3 Cd2 molecule Rattus norvegicus 121-124 10200422-8 1999 [3H]-NA release in response to low concentrations of nAChR agonists was fully inhibited by the presence of either TTX or Cd2+ (named TTX- or Cd2+-sensitive release). Tritium 1-3 Cd2 molecule Rattus norvegicus 141-144 10215666-5 1999 The potency of drugs at blocking [3H]neurotransmitter uptake was highly correlated with potency at blocking radioligand binding for hDAT and hSERT. Tritium 34-36 solute carrier family 6 member 3 Homo sapiens 132-136 10191274-3 1999 We now report that the non-cross-linked backbone of CRP, monomeric CRP (mCRP), stimulates the tyrosine phosphorylation of Syk and PLCgamma2 in platelets and induces the weak secretion of [3H]5-hydroxytryptamine ([3H]5-HT) and aggregation. Tritium 188-190 spleen associated tyrosine kinase Homo sapiens 122-125 10193663-0 1999 Characterisation of the 5-HT receptor binding profile of eletriptan and kinetics of [3H]eletriptan binding at human 5-HT1B and 5-HT1D receptors. Tritium 85-87 5-hydroxytryptamine receptor 1D Homo sapiens 127-133 10193663-3 1999 Kinetic studies comparing the binding of [3H]eletriptan and [3H]sumatriptan to the human recombinant 5-HT1B and 5-HT1D receptors expressed in HeLa cells revealed that both radioligands bound with high specificity (>90%) and reached equilibrium within 10-15 min. Tritium 42-44 5-hydroxytryptamine receptor 1D Homo sapiens 112-118 10193663-3 1999 Kinetic studies comparing the binding of [3H]eletriptan and [3H]sumatriptan to the human recombinant 5-HT1B and 5-HT1D receptors expressed in HeLa cells revealed that both radioligands bound with high specificity (>90%) and reached equilibrium within 10-15 min. Tritium 61-63 5-hydroxytryptamine receptor 1D Homo sapiens 112-118 11044737-0 2000 Characterization of nicotinic acetylcholine receptor-mediated [(3)H]-dopamine release from rat cortex and striatum. Tritium 64-67 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 20-52 9837923-5 1998 ATP-dependent [3H]GSH transport was cis-inhibited by substrates of the yeast Ycf1p transporter and inhibited by 4,4"-diisothiocyanatostilbene-2,2"-disulfonic acid, probenecid, and sulfinpyrazone, inhibitors of MRP1 and MRP2, but was minimally affected by membrane potential or pH gradient uncouplers. Tritium 15-17 mitochondrial 37S ribosomal protein MRP1 Saccharomyces cerevisiae S288C 210-214 11130334-4 2000 In the parietal cortex, [3H]-vesamicol binding to the vesicular acetylcholine transporter was significantly lower in beta-amyloid protein-treated rats than in vehicle-treated rats, and was significantly correlated with the mean number of correct selections in the maze task of the first 5 days in the post-operative state. Tritium 25-27 solute carrier family 18 member A3 Rattus norvegicus 54-89 9836617-1 1998 A series of four racemic ring-substituted trans-2-(indol-3-yl)cyclopropylamine derivatives was synthesized and tested for affinity at the 5-HT1A receptor, by competition with [3H]-8-OH-DPAT in rat hippocampal homogenates, and for affinity at the agonist-labeled cloned human 5-HT2A, 5-HT2B, and 5-HT2C receptor subtypes. Tritium 176-178 5-hydroxytryptamine receptor 1A Homo sapiens 138-153 11130334-5 2000 These results indicate that the reduction in [3H]-vesamicol binding to vesicular acetylcholine transporter is related to memory impairment induced by beta-amyloid protein. Tritium 46-48 solute carrier family 18 member A3 Rattus norvegicus 71-106 10206323-5 1999 The intracellular accumulations of [3H]vinblastine and BTL by LV500 cells were lower than those by L cells, but were significantly increased by verapamil, a P-gp inhibitor. Tritium 36-38 phosphoglycolate phosphatase Mus musculus 157-161 10218646-12 1999 Cyclocommunin reduced the [3H]phorbol 12,13-dibutyrate ([3H]PDB) binding to cytosolic PKC in a concentration-dependent manner. Tritium 26-29 protein kinase C, beta Rattus norvegicus 86-89 9843686-4 1998 In this study, we show that mammalian p10/NTF2 dramatically inhibits the dissociation of [3H]GDP from Ran and the binding of [35S]GTPgammaS to Ran following the dissociation of non-radioactive GDP by RCC1, the only known mammalian guanine nucleotide exchange factor for Ran (Ran-GEF) [7]. Tritium 90-92 RAN, member RAS oncogene family Homo sapiens 102-105 10064745-11 1999 In summary, for each of our experiments, the level of lipoprotein lipase expression in skeletal muscle, heart, and/or adipose tissue influenced the amount of [3H]retinoid taken up from chylomicrons and/or their remnants. Tritium 159-161 lipoprotein lipase Mus musculus 54-72 11062737-2 2000 Here we describe the rat everted gut sac as a new in vitro model for quantitation of p-gp-mediated efflux of anti-cancer agents using [3H]vinblastine, [14C] doxorubicin and verapamil as reference compounds. Tritium 135-137 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 85-89 10435283-5 1998 In a radioreceptor assay with calf uterus cytosol as androgen receptor preparation, the specific binding of [3H]DHT, the endogenous ligand, was completely displaceable by increasing concentrations of 3,4-DCAc. Tritium 109-111 androgen receptor Bos taurus 53-70 14647642-5 2000 The median time in nasal CPAP was 60.3h. Tritium 37-39 centromere protein J Homo sapiens 25-29 10924015-7 2000 In contrast, [3H]8-OH-DPAT binding in the hippocampal CA1 and CA3 regions was decreased by -9% and -20% from the level of chronic ethanol-treated FH rat (p < 0.05) and returned to the control level seen in FH (naive) rats. Tritium 14-16 carbonic anhydrase 3 Rattus norvegicus 62-65 10882043-3 2000 The magnitude of the Ca2+ response was related to the amount of the fusion protein and the number of surface alpha2A-adrenoceptor binding sites as estimated by [3H]RX 821002 binding. Tritium 161-163 adrenoceptor alpha 2A Homo sapiens 109-129 10069808-9 1999 However, mcd4-174 cells have a severe defect in incorporation of [3H]inositol into proteins and accumulate several previously uncharacterized [3H]inositol-labeled lipids whose properties are consistent with their being GPI precursors. Tritium 66-68 mannose-ethanolamine phosphotransferase MCD4 Saccharomyces cerevisiae S288C 9-13 10069808-9 1999 However, mcd4-174 cells have a severe defect in incorporation of [3H]inositol into proteins and accumulate several previously uncharacterized [3H]inositol-labeled lipids whose properties are consistent with their being GPI precursors. Tritium 143-145 mannose-ethanolamine phosphotransferase MCD4 Saccharomyces cerevisiae S288C 9-13 10509736-6 1999 The cultures treated for 24 h with antisense oligos (CaB+PV) showed a significant decrease in [3H]-GABA uptake as compared with the cultures treated with lipofectin alone or with lipofectin + mismatched antisense oligos to CaB and PV mRNA. Tritium 95-97 calbindin 1 Mus musculus 53-56 10082277-3 1999 With D4.2- and D2L receptor-transfected cell membranes, [3H]PNU-101958 showed high dopamine D4 receptor affinity and selectivity, and potent inhibition by dopamine D4 receptor-selective compounds. Tritium 57-59 dopamine receptor D4 Rattus norvegicus 83-103 10082277-3 1999 With D4.2- and D2L receptor-transfected cell membranes, [3H]PNU-101958 showed high dopamine D4 receptor affinity and selectivity, and potent inhibition by dopamine D4 receptor-selective compounds. Tritium 57-59 dopamine receptor D4 Rattus norvegicus 155-175 9792674-3 1998 MCP-1 showed binding to [3H]heparin with a KD of 1.5 microM. Tritium 25-27 C-C motif chemokine ligand 2 Homo sapiens 0-5 10215322-10 1999 Treatment of HK-2 cells during the recovery period after injury with an anti-PTHrP(1-36) antibody (at a dilution of 1:250) resulted in significant reductions in cell number and uptake of [3H]thymidine, compared with nonimmune serum at the same titer. Tritium 188-190 parathyroid hormone-like hormone Rattus norvegicus 77-82 10905634-7 2000 [3H]DHA uptake was more rapid in the cell bodies than in the NGC. Tritium 1-3 chondroitin sulfate proteoglycan 5 Rattus norvegicus 61-64 10752478-5 2000 Peritumoral injection of various kinds of adjuvant, particularly Freund"s complete adjuvant (FCA) plus bacillus Calmetee-Guerin (BCG), resulted in a marked accumulation of [3H]A-NK cells in mammary tumor tissues 24 h after injection, and simultaneously in the formation of vessels resembling high-endothelium venules (HEV), infiltration of LFA-1+ lymphocytes and expression of the ICAM-1 molecule on the tumor cells in the sites of tumor tissues. Tritium 173-175 intercellular adhesion molecule 1 Rattus norvegicus 381-387 9848395-7 1998 Rb1 (300 microg) pretreatment strongly inhibited the incorporation of the [3H]methyl moiety into phospholipids. Tritium 75-77 LOW QUALITY PROTEIN: retinoblastoma-associated protein Cavia porcellus 0-3 10737412-2 2000 In the present study equilibrium binding parameters of peripheral benzodiazepine receptors (PBRs) labelled by the specific radioligand [3H]PK 11195, were evaluated in cardiac tissue of rats submitted to 6 or 12 h noise exposure and of rats treated "in vivo" with PBR ligands such as PK 11195, Ro54864, diazepam and then noise-exposed. Tritium 136-138 translocator protein Rattus norvegicus 92-95 10728777-9 2000 However, the estimated binding affinity of [3H]TCDD to nuclear AhR from rat hepatocytes was similar. Tritium 44-46 aryl hydrocarbon receptor Rattus norvegicus 63-66 10083843-1 1999 We investigated the effect of a new PAF antagonist pinusolide, isolated from the leaves of Biota orientalis, on PAF-induced [3H]serotinin release from rabbit platelets, hypotension and vascular permeability. Tritium 125-127 PCNA clamp associated factor Rattus norvegicus 112-115 9931468-3 1999 Anti-sense plap oligonucleotide blocked cholera toxin-induced release of 3H-labeled arachidonic acid from cells, indicating a potential role for PLAP in regulating phospholipase A2 activity. Tritium 73-75 phospholipase A2 activating protein Homo sapiens 11-15 9931468-3 1999 Anti-sense plap oligonucleotide blocked cholera toxin-induced release of 3H-labeled arachidonic acid from cells, indicating a potential role for PLAP in regulating phospholipase A2 activity. Tritium 73-75 phospholipase A2 activating protein Homo sapiens 145-149 9776313-8 1998 Miconazole, another N-substituted imidazole, also stimulated [3H]IP1 accumulation in U373 cells. Tritium 62-64 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 65-68 10698291-2 1999 With [5-(3)H]dUTP used as the substrate, dUTPase, converts it to the corresponding monophosphate and is coupled with thymidylate synthase-catalyzed reaction, resulting in tritium release from [5-(3)H]dUMP. Tritium 171-178 Deoxyuridine triphosphatase Drosophila melanogaster 41-48 10674345-3 2000 First, CNP stimulates chondrocyte proliferation in the proliferative zone as assessed by [3H]thymidine incorporation. Tritium 90-92 natriuretic peptide C Rattus norvegicus 7-10 9748297-9 1998 The increases in GLUT4 at the cell surface induced by insulin or muscle contractions, measured with the 3H-labeled 2-N-4-(1-azi-2,2, 2-trifluoroethyl)-benzoyl-1,3-bis-(D-mannose-4-yloxy)-2-propyla min e photolabel, were 26-36% smaller in muscles of the 8-week high fat-fed rats as compared with control rats. Tritium 104-106 solute carrier family 2 member 4 Rattus norvegicus 17-22 11741243-9 2000 [3H]-Cyclosporin A uptake by TM-BBB was significantly increased in the presence of 100 micromol/l verapamil and vincristine, suggesting that TM-BBB exhibits efflux transport activity via P-gp. Tritium 1-3 phosphoglycolate phosphatase Mus musculus 187-191 9886967-5 1999 [3H]DHEA bound to purified PKC containing PKC-alpha, -beta, and -gamma. Tritium 1-3 protein kinase C, alpha Rattus norvegicus 27-30 9886967-5 1999 [3H]DHEA bound to purified PKC containing PKC-alpha, -beta, and -gamma. Tritium 1-3 protein kinase C, alpha Rattus norvegicus 42-70 9887008-4 1999 Both vasoactive intestinal polypeptide (VIP) and pituitary adenylate cyclase-activating peptide-(1-38) [PACAP-(1-38)] concentration dependently (10(-10) to 10(-6) M) inhibited the serum-induced [3H]thymidine incorporation [in colon, 48.2 +/- 5.8 and 55.6 +/- 9.3% of control with 10(-6) M VIP and 10(-7) M PACAP-(1-38)] and inhibited increase in cell numbers in cultures derived from the colon but not in those from the antrum. Tritium 195-197 LOW QUALITY PROTEIN: pituitary adenylate cyclase-activating polypeptide Oryctolagus cuniculus 104-109 10645886-6 2000 [(3)H]choline was released when natural surfactant containing [(3)H]choline-labeled DPPC was cycled with yeast phospholipase D but not with convertase or peanut and cabbage phospholipases D. Similarly, yeast phospholipase D hydrolyzed [(3)H]choline from [(3)H]choline-labeled DPPC after incubation in vitro, whereas convertase, liver esterase, or peanut and cabbage phospholipases D did not. Tritium 2-5 phospholipase D Saccharomyces cerevisiae S288C 111-126 10211703-1 1998 P-selectin glycoprotein ligand-1, PSGL-1, a specific ligand for P-, E-, and L-selectin, was isolated from in vivo [3H]-glucosamine labeled HL-60 cells by a combination of wheat germ agglutinin and platelet P-selectin- or E-selectin receptor globulin-agarose chromatography. Tritium 115-117 selectin P ligand Homo sapiens 0-32 10645886-6 2000 [(3)H]choline was released when natural surfactant containing [(3)H]choline-labeled DPPC was cycled with yeast phospholipase D but not with convertase or peanut and cabbage phospholipases D. Similarly, yeast phospholipase D hydrolyzed [(3)H]choline from [(3)H]choline-labeled DPPC after incubation in vitro, whereas convertase, liver esterase, or peanut and cabbage phospholipases D did not. Tritium 2-5 phospholipase D Saccharomyces cerevisiae S288C 208-223 10645886-6 2000 [(3)H]choline was released when natural surfactant containing [(3)H]choline-labeled DPPC was cycled with yeast phospholipase D but not with convertase or peanut and cabbage phospholipases D. Similarly, yeast phospholipase D hydrolyzed [(3)H]choline from [(3)H]choline-labeled DPPC after incubation in vitro, whereas convertase, liver esterase, or peanut and cabbage phospholipases D did not. Tritium 1-5 phospholipase D Saccharomyces cerevisiae S288C 111-126 10645886-6 2000 [(3)H]choline was released when natural surfactant containing [(3)H]choline-labeled DPPC was cycled with yeast phospholipase D but not with convertase or peanut and cabbage phospholipases D. Similarly, yeast phospholipase D hydrolyzed [(3)H]choline from [(3)H]choline-labeled DPPC after incubation in vitro, whereas convertase, liver esterase, or peanut and cabbage phospholipases D did not. Tritium 1-5 phospholipase D Saccharomyces cerevisiae S288C 208-223 10645886-6 2000 [(3)H]choline was released when natural surfactant containing [(3)H]choline-labeled DPPC was cycled with yeast phospholipase D but not with convertase or peanut and cabbage phospholipases D. Similarly, yeast phospholipase D hydrolyzed [(3)H]choline from [(3)H]choline-labeled DPPC after incubation in vitro, whereas convertase, liver esterase, or peanut and cabbage phospholipases D did not. Tritium 63-67 phospholipase D Saccharomyces cerevisiae S288C 111-126 10651144-2 2000 CCK-8S in a concentration dependent manner (10-3000 nM) increased K+-evoked [3H]GABA release (EC50=192 nM). Tritium 77-79 cholecystokinin Rattus norvegicus 0-3 10651144-5 2000 CCK-8S-induced increase in [3H]GABA overflow was completely blocked by 1 microM tetrodotoxin. Tritium 28-30 cholecystokinin Rattus norvegicus 0-3 10651144-10 2000 The data presented suggest that CCK(B) receptors modulating [3H]GABA release from anterior accumbal punches may not be present on GABAergic terminals but could be located on glutamatergic interneurons. Tritium 61-63 cholecystokinin Rattus norvegicus 32-35 10612708-6 1999 Further, experiments on cultured cells transfected with nociceptin receptor cDNA show that NalBzoH competes [3H]-nociceptin binding and attenuates the nociceptin-induced inhibition of cAMP accumulation induced by forskolin. Tritium 109-111 opioid receptor-like 1 Mus musculus 56-75 10051123-4 1999 CCK-8s, gastrin(1-17) and its glycine-extended precursor G(1-17)-Gly, previously reported to cause proliferation via putative novel sites on AR4-2J and Swiss 3T3 cells, elicited significant dose dependent increases of similar magnitude in [3H]thymidine incorporation over 3 days in serum-free medium of 39 +/- 10% (P < 0.01, n = 20), 37 +/- 8% (P < 0.01, n = 27) and 41 +/- 6% (P < 0.01, n = 36) respectively. Tritium 240-242 cholecystokinin Rattus norvegicus 0-3 10421431-4 1999 With the addition of ISDN to the culture medium, 3H-labeled mucin in the full-thickness corpus mucosa increased to 124-145% of the control (p<0.05), but not in the antrum. Tritium 49-51 solute carrier family 13 member 2 Rattus norvegicus 60-65 10579463-4 1999 Ro5-4864 (IC50 17.3 nM) and PK 11195 (IC50 12.3 nM) were able to compete with [3H]Ro5-4864 for binding, indicating specificity of interaction with PBR. Tritium 79-81 translocator protein Homo sapiens 147-150 9933145-3 1999 In mouse brain cortex membranes, the binding of the ORL1 receptor agonist [3H]nociceptin was equipotently inhibited by Mr 2266 and its enantiomer Mr 2267 (pK(i) 4.82 and 5.14, respectively), whereas the binding of the kappa-opioid receptor agonist [3H]U-69,593 was inhibited by Mr 2266 more potently (pK(i) 9.11) than by its enantiomer Mr 2267 (pK(i)7.15). Tritium 75-77 opioid receptor-like 1 Mus musculus 52-56 9933145-3 1999 In mouse brain cortex membranes, the binding of the ORL1 receptor agonist [3H]nociceptin was equipotently inhibited by Mr 2266 and its enantiomer Mr 2267 (pK(i) 4.82 and 5.14, respectively), whereas the binding of the kappa-opioid receptor agonist [3H]U-69,593 was inhibited by Mr 2266 more potently (pK(i) 9.11) than by its enantiomer Mr 2267 (pK(i)7.15). Tritium 249-251 opioid receptor-like 1 Mus musculus 52-56 10555746-5 1999 Similarly, the rate at which [3H]vinblastine associates with P-gp was indistinguishable between the two isoforms. Tritium 30-32 phosphoglycolate phosphatase Mus musculus 61-65 10211703-1 1998 P-selectin glycoprotein ligand-1, PSGL-1, a specific ligand for P-, E-, and L-selectin, was isolated from in vivo [3H]-glucosamine labeled HL-60 cells by a combination of wheat germ agglutinin and platelet P-selectin- or E-selectin receptor globulin-agarose chromatography. Tritium 115-117 selectin P ligand Homo sapiens 34-40 10193907-3 1999 In NAT-cells amphetamine stimulated [3H]noradrenaline efflux concentration-dependently when added to the superfusion buffer at 0.01, 0.1 and 1 microM. Tritium 37-39 bromodomain containing 2 Homo sapiens 3-6 9922982-3 1998 Effect of MX1 (10(-6) M) on mucin biosynthesis measured by [3H] glucosamine incorporation in rat gastric corpus has been determined. Tritium 60-62 MX dynamin like GTPase 1 Rattus norvegicus 10-13 10447088-4 1999 A [3H]thymidine incorporation study showed that SS significantly and concentration-dependently inhibited HL60 and BALL-1 leukemia cell growth. Tritium 3-5 somatostatin Homo sapiens 48-50 10556940-15 1999 However, Glu-lac showed a good correlation between its potency to stimulate the [gamma-35S]-GTP binding and its affinity for displacement of [3H]-L-AP4 binding. Tritium 142-144 replication initiator 1 Rattus norvegicus 148-151 10510280-0 1999 Tritium secondary kinetic isotope effect on phenylalanine ammonia-lyase-catalyzed reaction. Tritium 0-7 peptidylglycine alpha-amidating monooxygenase Homo sapiens 44-71 10501211-3 1999 TNF-alpha produced a phosphorylation of cPLA2, which was preceded by an activation of both c-Jun N-terminal kinase (JNK) and p38-MAP kinase, and this was associated with the release of [3H]AA. Tritium 186-188 phospholipase A2 group IVA Homo sapiens 40-45 9771908-7 1998 Treatment of TFG2 cells with the specific MEKI inhibitor PD98059, or infection with a -/- MEK1 recombinant adenovirus permitted phenylephrine to increase rather than decrease [3H]thymidine incorporation. Tritium 176-178 mitogen-activated protein kinase kinase 1 Homo sapiens 90-94 10551275-1 1999 The effect of the native and rodent-selective 5-HT1B receptor agonists (5-hydroxytryptamine (5-HT) and CP93,129) on the K+-evoked overflows of [3H]5-HT, [3H]dopamine (DA) and [3H]acetylcholine (ACh) was studied in synaptosome preparations obtained from rat brain striatum or hippocampus loaded with radiolabeled neurotransmitter. Tritium 144-146 5-hydroxytryptamine receptor 1B Rattus norvegicus 46-52 10551275-4 1999 The inhibitory effects of the 5-HT1B receptor agonists on [3H] K+-overflows were antagonized by the selective 5-HT1B receptor antagonist (SB224289), further indicating that the observed effects were 5-HT1B receptor specific. Tritium 59-61 5-hydroxytryptamine receptor 1B Rattus norvegicus 30-36 10551275-4 1999 The inhibitory effects of the 5-HT1B receptor agonists on [3H] K+-overflows were antagonized by the selective 5-HT1B receptor antagonist (SB224289), further indicating that the observed effects were 5-HT1B receptor specific. Tritium 59-61 5-hydroxytryptamine receptor 1B Rattus norvegicus 110-116 10437122-3 1999 RESULTS: C-sis antisense ODN 2, 4, 6, 8, and 10 mumol.L-1 inhibited VSMC proliferation (10.3% +/- 0.7%, 22.6% +/- 0.9%, 31.0% +/- 1.1%, 35.4% +/- 0.9%, and 43.3% +/- 1.2%) and [3H]TdR incorporation (6.8% +/- 0.3%, 9.7% +/- 0.7%, 29.0% +/- 0.6%, 32.0% +/- 0.7%, and 50.6% +/- 1.3%) in a concentration-dependent manner. Tritium 177-179 platelet derived growth factor subunit B Homo sapiens 9-14 9843851-6 1998 Data of the elastinolytic activities of the neutrophils and macrophages, determined in [3H]elastin-coated culture wells, showed that the elastinolytic activity of lung neutrophils in the smoke-exposed rats was similar to that of the control air-exposed rats at months 1-6 of exposure. Tritium 88-90 elastin Rattus norvegicus 12-19 9884077-1 1998 The present study describes the labelling of the nociceptin (NC) receptor, ORL1, in mouse forebrain membranes with a new ligand partially protected from metabolic degradation at the C-terminal; the ligand, [3H]-NC-NH2, has a specific activity of 24.5 Ci mmol(-1). Tritium 207-209 opioid receptor-like 1 Mus musculus 49-73 9884077-1 1998 The present study describes the labelling of the nociceptin (NC) receptor, ORL1, in mouse forebrain membranes with a new ligand partially protected from metabolic degradation at the C-terminal; the ligand, [3H]-NC-NH2, has a specific activity of 24.5 Ci mmol(-1). Tritium 207-209 opioid receptor-like 1 Mus musculus 75-79 9884077-5 1998 Thermodynamic analyses indicated that [3H]-NC-NH2 binding to the mouse ORL1 is totally entropy driven, similar to what has been observed for the labelled agonists to the opioid receptors OP1(delta), OP2(kappa) and OP3(mu). Tritium 39-41 opioid receptor-like 1 Mus musculus 71-75 10551275-4 1999 The inhibitory effects of the 5-HT1B receptor agonists on [3H] K+-overflows were antagonized by the selective 5-HT1B receptor antagonist (SB224289), further indicating that the observed effects were 5-HT1B receptor specific. Tritium 59-61 5-hydroxytryptamine receptor 1B Rattus norvegicus 110-116 10498194-2 1999 The [3H]phorbol-12,13-dibutyrate (PDBu) binding to PKD-C1A was affected drastically by incubation temperature while that to PKD-C1B was not. Tritium 4-8 protein kinase D1 Homo sapiens 51-58 10498194-2 1999 The [3H]phorbol-12,13-dibutyrate (PDBu) binding to PKD-C1A was affected drastically by incubation temperature while that to PKD-C1B was not. Tritium 4-8 protein kinase D1 Homo sapiens 51-54 10498194-3 1999 Scatchard analysis of [3H]PDBu binding to both PKD C1 peptides gave dissociation constants of 2.5 +/- 0.4 and 2.7 +/- 0.8 nM for PKD-C1A and PKD-C1B, respectively, indicating that the two cysteine-rich motifs of PKD are functionally equivalent like those of PKCgamma. Tritium 23-25 protein kinase D1 Homo sapiens 47-50 10498194-3 1999 Scatchard analysis of [3H]PDBu binding to both PKD C1 peptides gave dissociation constants of 2.5 +/- 0.4 and 2.7 +/- 0.8 nM for PKD-C1A and PKD-C1B, respectively, indicating that the two cysteine-rich motifs of PKD are functionally equivalent like those of PKCgamma. Tritium 23-25 protein kinase D1 Homo sapiens 129-136 10498194-3 1999 Scatchard analysis of [3H]PDBu binding to both PKD C1 peptides gave dissociation constants of 2.5 +/- 0.4 and 2.7 +/- 0.8 nM for PKD-C1A and PKD-C1B, respectively, indicating that the two cysteine-rich motifs of PKD are functionally equivalent like those of PKCgamma. Tritium 23-25 protein kinase D1 Homo sapiens 129-132 10498194-3 1999 Scatchard analysis of [3H]PDBu binding to both PKD C1 peptides gave dissociation constants of 2.5 +/- 0.4 and 2.7 +/- 0.8 nM for PKD-C1A and PKD-C1B, respectively, indicating that the two cysteine-rich motifs of PKD are functionally equivalent like those of PKCgamma. Tritium 23-25 protein kinase D1 Homo sapiens 129-132 10070309-7 1998 Furthermore, IFN-alpha showed a significant antiproliferative effect (by [3H]thymidine incorporation) on two carcinoid tumour cell lines in a dose-dependent manner which correlated with a dose-dependent induction of p68 mRNA and protein expression (by Northern and Western blot analysis). Tritium 74-76 KH RNA binding domain containing, signal transduction associated 1 Homo sapiens 216-219 9802410-7 1998 NPY increased [3H]thymidine incorporation by 80% after a 24-h treatment in a bimodal fashion, with peak effects at 10(-10) M and 10(-8) M. Gp120 was an even more potent mitogen for VSMCs with peak activity occurring at 10(-12) M. Peptide T was equipotent with gp120, and slightly less efficacious, suggesting that this domain may mediate gp120 effects on VSMCs. Tritium 15-17 neuropeptide Y Homo sapiens 0-3 9814825-8 1998 Raising Ca(0)2+ from 1.8 to 4.8 mM or addition of the polycationic CaR agonist neomycin (300 microM) modestly but significantly increased [3H]-thymidine incorporation into oligodendrocytes. Tritium 139-141 nuclear receptor subfamily 1, group I, member 3 Rattus norvegicus 67-70 9864301-2 1998 [3H]5-CT binding was inhibited by cyanopindolol with an IC50 of 0.87 +/- 0.30 nM, suggesting the expression of the 5-HT1B receptor in these neurons. Tritium 1-3 5-hydroxytryptamine receptor 1B Rattus norvegicus 115-121 10615423-8 1999 A cAMP analogue, 8-bromo-cAMP, mimicked the AM effects, inhibiting the Ang II-stimulated [3H]thymidine and [3H]phenylalanine incorporation. Tritium 90-92 angiogenin Rattus norvegicus 71-74 10615423-8 1999 A cAMP analogue, 8-bromo-cAMP, mimicked the AM effects, inhibiting the Ang II-stimulated [3H]thymidine and [3H]phenylalanine incorporation. Tritium 108-110 angiogenin Rattus norvegicus 71-74 9864301-4 1998 5-HT1B receptors are the predominant type of serotonin receptors labeled by [3H]5-HT in cultured DRG neurons, representing approximately 60% of the specific [3H]5-HT binding sites. Tritium 77-79 5-hydroxytryptamine receptor 1B Rattus norvegicus 0-6 10415092-4 1999 Here we present a high-yield method for the synthesis of [(3)H]UDP-GalA from [(3)H]UTP and Glc-1-P by sequential reactions catalyzed by UDP-Glc pyrophosphorylase, UDP-Glc dehydrogenase, and UDP-GlcA-4-epimerase and the separation of the reaction products over a Dionex CarboPac PA1 anion-exchange column using high-performance anion-exchange chromatography (HPAEC). Tritium 57-62 galactosidase alpha Homo sapiens 67-71 9802410-7 1998 NPY increased [3H]thymidine incorporation by 80% after a 24-h treatment in a bimodal fashion, with peak effects at 10(-10) M and 10(-8) M. Gp120 was an even more potent mitogen for VSMCs with peak activity occurring at 10(-12) M. Peptide T was equipotent with gp120, and slightly less efficacious, suggesting that this domain may mediate gp120 effects on VSMCs. Tritium 15-17 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 139-144 9864301-4 1998 5-HT1B receptors are the predominant type of serotonin receptors labeled by [3H]5-HT in cultured DRG neurons, representing approximately 60% of the specific [3H]5-HT binding sites. Tritium 158-160 5-hydroxytryptamine receptor 1B Rattus norvegicus 0-6 9727016-6 1998 Hyperosmotic sucrose inhibited internalization of VSV-TRHR-GFP, measured both by intact cell [3H]TRH binding studies and by confocal microscopy. Tritium 94-96 thyrotropin releasing hormone receptor Rattus norvegicus 54-58 9929613-6 1998 (ii) tritiated thymidine [3H]TdR autoradiographic birthdating studies show that the vast majority of 2A4(+) ORNs present at P21, when adult 2A4(+) ORN densities are first observed, were "born" postnatally, between P3 and P10. Tritium 26-28 KRAS proto-oncogene, GTPase Rattus norvegicus 124-127 9822636-8 1998 Metabolic labeling with [3H]myristic acid showed that both the 120- and 160-kDa kinectin are myristoylated in chick embryo fibroblasts. Tritium 25-27 kinectin 1 Gallus gallus 80-88 10389844-3 1999 The biosynthesis and secretion of proinsulin and insulin were analyzed by comparing the incorporation of [3H]Leu and [35S]Met. Tritium 106-108 insulin II Mus musculus 34-44 9788776-5 1998 In contrast, EP2 and EP4 receptors bearing conservative threonine to serine mutations (EP2T185S; EP4T168S) displayed Kd values for [3H]prostaglandin E2 similar to wild type receptors: 8.8 +/- 0.7 nM for EP2T185S compared to 12.9 +/- 1.2 nM for EP2 wild type; 2.0 +/- 0.8 nM for EP4T168S compared to 0.9 +/- 0.3 nM for the EP4 wild type receptor. Tritium 132-134 prostaglandin E receptor 4 Homo sapiens 21-24 10393324-4 1999 While both DMS and DL-threo-DHS inhibited not only [3H]Sph-1-P formation but also protein kinase C-dependent platelet aggregation, staurosporine, a potent protein kinase inhibitor, only inhibited the protein kinase C-dependent reaction. Tritium 52-54 ankyrin 1 Homo sapiens 55-60 10381762-9 1999 BIM completely displaced binding of the selective 5-HT3 receptor antagonist [3H]GR65630. Tritium 77-79 BCL2-like 11 (apoptosis facilitator) Mus musculus 0-3 10358090-6 1999 In planar lipid bilayers, RyR3 displayed cation channel activity that was modulated by several ligands including Ca2+, Mg2+, caffeine, and ATP, which is consistent with [3H]ryanodine binding activity. Tritium 170-172 ryanodine receptor 3 Oryctolagus cuniculus 26-30 9840289-2 1998 The study was carried out by analyzing the activity of the two drugs on: (1) extracellular and intracellular Tat protein, introduced into HL3T1 cells containing an integrated HIV-1 LTR/CAT plasmid; (2) binding of Tat to 3H-labeled heparin and to 14C-labeled PNU145156E; and (3) the angiogenic response induced in vivo by culture medium conditioned by T53c14 cells, which release extracellular Tat. Tritium 220-222 Tat Human immunodeficiency virus 1 213-216 9840289-2 1998 The study was carried out by analyzing the activity of the two drugs on: (1) extracellular and intracellular Tat protein, introduced into HL3T1 cells containing an integrated HIV-1 LTR/CAT plasmid; (2) binding of Tat to 3H-labeled heparin and to 14C-labeled PNU145156E; and (3) the angiogenic response induced in vivo by culture medium conditioned by T53c14 cells, which release extracellular Tat. Tritium 220-222 Tat Human immunodeficiency virus 1 213-216 9845281-1 1998 In this study, the binding of [3H]5-HT to the cloned dog 5-hydroxytryptamine1B (dog 5-HT1B) receptor, stably expressed in Chinese hamster ovary cells (ATCC CCL 61)(CHO-K1), was characterised and its pharmacology compared with that of the cloned human and rat 5-HT1B receptors. Tritium 31-33 5-hydroxytryptamine receptor 1B Rattus norvegicus 84-90 9845281-1 1998 In this study, the binding of [3H]5-HT to the cloned dog 5-hydroxytryptamine1B (dog 5-HT1B) receptor, stably expressed in Chinese hamster ovary cells (ATCC CCL 61)(CHO-K1), was characterised and its pharmacology compared with that of the cloned human and rat 5-HT1B receptors. Tritium 31-33 5-hydroxytryptamine receptor 1B Rattus norvegicus 259-265 9845281-2 1998 [3H]5-HT specifically labeled, with high affinity, an apparently homogeneous population of binding sites in the dog 5-HT1B receptor cell line yielding a pKd of 8.1. Tritium 1-3 5-hydroxytryptamine receptor 1B Rattus norvegicus 116-122 9751179-4 1998 NMDA-specific [3H]homoquinolinate binding was low in brain regions that express NR2C and NR2D mRNA (cerebellar granular cell layer, NR2C; glomerular layer of olfactory bulb, NR2C/NR2D; and midline thalamic nuclei, NR2D). Tritium 15-17 glutamate receptor, ionotropic, NMDA2D (epsilon 4) Mus musculus 89-93 9751179-4 1998 NMDA-specific [3H]homoquinolinate binding was low in brain regions that express NR2C and NR2D mRNA (cerebellar granular cell layer, NR2C; glomerular layer of olfactory bulb, NR2C/NR2D; and midline thalamic nuclei, NR2D). Tritium 15-17 glutamate receptor, ionotropic, NMDA2D (epsilon 4) Mus musculus 179-183 9751179-4 1998 NMDA-specific [3H]homoquinolinate binding was low in brain regions that express NR2C and NR2D mRNA (cerebellar granular cell layer, NR2C; glomerular layer of olfactory bulb, NR2C/NR2D; and midline thalamic nuclei, NR2D). Tritium 15-17 glutamate receptor, ionotropic, NMDA2D (epsilon 4) Mus musculus 179-183 10357798-6 1999 We examined the ability of the expressed mGSTA3-3 to protect against AFB1-induced cytotoxicity or [3H]-covalent adduct formation in cellular nucleic acids. Tritium 99-101 glutathione S-transferase, alpha 3 Mus musculus 41-49 10357798-7 1999 Exposure of empty vector-transfected control cells and mGSTA3-3 expressing cells to up to 600 nM [3H]-AFB1 indicated that a 70-80% reduction in DNA and RNA adducts was afforded by the expression of mGSTA3-3 in the transfected cells. Tritium 98-100 glutathione S-transferase, alpha 3 Mus musculus 198-204 10352099-4 1999 Treatment with PTHrP(107-139) for 24 h caused a dose-dependent increase in cell number, [3H]thymidine and [3H]phenylalanine incorporation in cultured osteoblasts. Tritium 89-91 parathyroid hormone-like hormone Rattus norvegicus 15-20 9788776-5 1998 In contrast, EP2 and EP4 receptors bearing conservative threonine to serine mutations (EP2T185S; EP4T168S) displayed Kd values for [3H]prostaglandin E2 similar to wild type receptors: 8.8 +/- 0.7 nM for EP2T185S compared to 12.9 +/- 1.2 nM for EP2 wild type; 2.0 +/- 0.8 nM for EP4T168S compared to 0.9 +/- 0.3 nM for the EP4 wild type receptor. Tritium 132-134 prostaglandin E receptor 4 Homo sapiens 97-100 10352099-4 1999 Treatment with PTHrP(107-139) for 24 h caused a dose-dependent increase in cell number, [3H]thymidine and [3H]phenylalanine incorporation in cultured osteoblasts. Tritium 107-109 parathyroid hormone-like hormone Rattus norvegicus 15-20 9724265-3 1998 Epidermal growth factor (EGF) stimulated cell growth, [3H]glucosamine uptake, and accumulation of mucus granules positive for galactose oxidase-Schiff reaction. Tritium 55-57 pro-epidermal growth factor Cavia porcellus 0-23 10397516-8 1999 RESULTS: We demonstrated that r40-kDa OMP Ab in the presence of human complement successfully opsonized [3H]-thymidine-labeled P. gingivalis as a target for phagocytosis by HL-60 cells differentiated with dimethyl sulfoxide. Tritium 105-107 olfactory marker protein Homo sapiens 38-41 9704884-3 1998 As with both native neuronal and cloned DATs, acute exposure of hDAT expressing Sf9 cells to the PKC activator PMA (1 microM), but not alphaPDD, reduced the Vmax (approximately 1 pmol/min/10(5) cells) for [3H]DA uptake by approximately 40%, an effect which was blocked by the protein kinase inhibitor staurosporine. Tritium 206-208 solute carrier family 6 member 3 Homo sapiens 64-68 9704884-8 1998 Immunofluorescent confocal microscopy demonstrated that the observed functional consequence of PKC activation on [3H]DA uptake is associated with the rapid sequestration/internalization of hDAT protein from the cell surface, while the increase in DA uptake following PKC/PKA inhibition is the result of the recruitment of internalized or intracellular transporters to the plasma membrane. Tritium 114-116 solute carrier family 6 member 3 Homo sapiens 189-193 9754915-5 1998 The hippocampal CA3 sector also showed a significant elevation (32-40%) in [3H]L-N(G)-nitroarginine binding at 24 and 48 h after global ischemia. Tritium 76-78 carbonic anhydrase 3 Rattus norvegicus 16-19 9733635-5 1998 RESULTS: In short-term culture, HGF stimulated [3H]thymidine incorporation in a dose-dependent manner from a threshold at 10 pM to a maximum at 100 pM. Tritium 48-50 hepatocyte growth factor Oryctolagus cuniculus 32-35 9733635-7 1998 For HGF, maximal [3H]thymidine incorporation was 3.6 +/- 0.7 times basal. Tritium 18-20 hepatocyte growth factor Oryctolagus cuniculus 4-7 9733635-8 1998 For EGF and TGF-alpha, maximal [3H]thymidine incorporation was 4.3 +/- 0.4, and 3.6 +/- 0.4 times basal, respectively. Tritium 32-34 protransforming growth factor alpha Oryctolagus cuniculus 12-21 9724265-3 1998 Epidermal growth factor (EGF) stimulated cell growth, [3H]glucosamine uptake, and accumulation of mucus granules positive for galactose oxidase-Schiff reaction. Tritium 55-57 pro-epidermal growth factor Cavia porcellus 25-28 9733635-14 1998 CONCLUSIONS: Our results suggest that: (1) HGF stimulates [3H]thymidine uptake and cell proliferation in fetal rabbit gastric epithelial cells in vitro, and (2) HGF"s mitogenic effect on fetal rabbit gastric epithelial cell growth is comparable to that observed for EGF and TGF-alpha, but superior to the effect observed for IGF-1. Tritium 59-61 hepatocyte growth factor Oryctolagus cuniculus 43-46 9726031-3 1998 DESIGN: The level and pattern of activity of MMP2 and MMP9 were investigated by measuring the degradation of 3H-labelled gelatin and by zymography in blister fluid from six TEN patients and compared the results with three other blistering conditions: bullous pemphigoid (n = 6), second-degree burn (n = 13) or suction blister (n = 3). Tritium 109-111 matrix metallopeptidase 9 Homo sapiens 54-58 9658403-12 1998 The nuclear 9S-[3H]Org2058-PR resulting from cells exposed to Rap, contained, in addition to the heat shock proteins of 90 kDa and 70 kDa (hsp90 and hsp70), the FK506-binding immunophilin FKBP52 but not FKBP51, although the latter was part of unliganded PR heterocomplex associated with hsp90. Tritium 16-18 heat shock protein family A (Hsp70) member 4 Homo sapiens 149-154 9690735-5 1998 The results showed that 1500 nM [3H]ouabain binding was sensitive to serotonin 10(-3) M and significantly increased in the following brain regions: frontal cortex, areas CA1, CA2, and CA3 of the hippocampus, presubiculum, zona incerta, caudate putamen and the amygdaloid area, confirming and extending previous results. Tritium 33-35 carbonic anhydrase 3 Rattus norvegicus 184-187 9633917-7 1998 The effect of myotrophin on the stimulation of PKC activity and [3H]leucine incorporation was abolished by pretreatment with either staurosporine or H-7, two selective, pharmacological PKC inhibitors. Tritium 65-67 protein kinase C, alpha Rattus norvegicus 185-188 10678145-5 1999 RESULTS: At a concentration range from 10(-12) to 10(-8) mol.L-1, ET-1 stimulated [3H]thymidine incorporation and phospho-p44/42 MAPK expression in VSMC in a concentration-dependent manner. Tritium 83-85 endothelin-1 Oryctolagus cuniculus 66-70 10374718-4 1999 Adrenomedullin also decreased [3H]thymidine incorporation and increased nucleosome-associated cytoplasmic DNA fragmentation, in a concentration-dependent fashion. Tritium 31-33 adrenomedullin Rattus norvegicus 0-14 10340305-8 1999 [3H]MLA, therefore, is a novel radiolabel for characterising alpha 7-type nAChR. Tritium 1-3 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 74-79 9764974-8 1998 Significant septotemporal gradients of [3H]MK-801 binding were observed in selected layers of CA1 and the dentate gyrus, a septal to temporal decrease of binding in the oriens and radiatum layers of CA1 being most prominent. Tritium 40-42 carbonic anhydrase 1 Rattus norvegicus 94-97 10336170-2 1999 Significant increases by 60-80% in [3H]L-AP4 binding was observed in the frontal parts of cortex (sensory, motor and cingulate cortex) and by 28% in the molecular layer of the cerebellar cortex 24 h after PTZ-induced seizures. Tritium 36-38 replication initiator 1 Rattus norvegicus 41-44 9689471-7 1998 [3H]IP3 binding in the CA1 region showed statistically significant declines with respect to both neurofibrillary changes and amyloid staging (P < 0.05). Tritium 1-3 carbonic anhydrase 1 Homo sapiens 23-26 9793613-4 1998 Sch 50971 inhibits [3H]-N-alpha-methylhistamine (CAS 673-50-7) binding to the histamine H3 receptor in human brain (Ki = 5.0 nmol/l) and guinea pig brain (Ki = 2.5 nmol/l). Tritium 20-22 histamine receptor H3 Homo sapiens 78-99 9689471-10 1998 In contrast, [3H]PDBu binding showed significant declines with neurofibrillary staging in the entorhinal region (P < 0.01), subiculum (P < 0.001), CA1 (P < 0.001), CA2 (P < 0.001), CA3 (P < 0.001) and CA4 (P < 0.0001) regions and the dentate gyrus (P < 0.0001). Tritium 14-16 carbonic anhydrase 1 Homo sapiens 147-150 9689471-10 1998 In contrast, [3H]PDBu binding showed significant declines with neurofibrillary staging in the entorhinal region (P < 0.01), subiculum (P < 0.001), CA1 (P < 0.001), CA2 (P < 0.001), CA3 (P < 0.001) and CA4 (P < 0.0001) regions and the dentate gyrus (P < 0.0001). Tritium 14-16 carbonic anhydrase 2 Homo sapiens 164-167 9618393-2 1998 In vitro CP-195543 inhibited [3H]LTB4 binding to high-affinity LTB4 receptors on human neutrophils (HN) and murine spleen membranes with IC50 values of 6.8 nM (Ki = 4.9 nM) and 37.0 nM (Ki = 26.9 nM), respectively. Tritium 30-32 prostaglandin reductase 1 Cavia porcellus 33-37 10208935-2 1999 This protein, designated gp250, was the sole viral glycoprotein detected in the culture medium after [3H]mannose labeling of the infected cells. Tritium 102-104 sortilin related receptor 1 Homo sapiens 25-30 10208935-7 1999 An important shift in the relative mobility of gp250 in SDS-PAGE was observed after tunicamycin treatment of infected cells labeled with [3H]glucosamine, confirming the presence of N-linked sugars on gp250. Tritium 137-141 sortilin related receptor 1 Homo sapiens 47-52 10101238-4 1999 This fits with the labelling by the 5-HT1B/1D receptor antagonist [3H] GR 125743. Tritium 67-69 5-hydroxytryptamine receptor 1B Rattus norvegicus 36-42 9618393-2 1998 In vitro CP-195543 inhibited [3H]LTB4 binding to high-affinity LTB4 receptors on human neutrophils (HN) and murine spleen membranes with IC50 values of 6.8 nM (Ki = 4.9 nM) and 37.0 nM (Ki = 26.9 nM), respectively. Tritium 30-32 prostaglandin reductase 1 Cavia porcellus 63-67 9618393-4 1998 Evidence of noncompetitive antagonist effects on the HN high-affinity LTB4 receptor was obtained by Scatchard analysis of [3H]LTB4 binding to and chemotaxis of HN to LTB4. Tritium 123-125 prostaglandin reductase 1 Cavia porcellus 70-74 9786498-7 1998 Cumulative concentrations (1-1000 nM) of EP-receptor agonists like prostaglandin E2, nocloprost, and sulprostone (EP1 and EP3 selective) inhibited evoked [3H]ACh release in a concentration dependent manner with IC50 values between 4- 14 nM and maximal inhibition of about 70%. Tritium 155-157 prostaglandin E receptor 1 Homo sapiens 114-117 9618393-4 1998 Evidence of noncompetitive antagonist effects on the HN high-affinity LTB4 receptor was obtained by Scatchard analysis of [3H]LTB4 binding to and chemotaxis of HN to LTB4. Tritium 123-125 prostaglandin reductase 1 Cavia porcellus 126-130 10464778-12 1999 Experiments on the cultured cells transfected with the nociceptin receptor cDNA showed that NalBzoH competed in [3H]-nociceptin binding and attenuated the nociceptin-induced inhibition of cyclic AMP accumulation induced by forskolin. Tritium 113-115 opioid receptor-like 1 Mus musculus 55-74 9820275-6 1998 Both insulin-like growth factor-I and transforming growth factor-beta resulted in significant elevations of [3H]thymidine uptake; however, cell numbers did not rise sufficiently over the 48-hour culture period to reach significant levels. Tritium 109-111 insulin like growth factor 1 Equus caballus 5-69 10037481-0 1999 Modeling of the interaction of Na+ and K+ with the binding of dopamine and [3H]WIN 35,428 to the human dopamine transporter. Tritium 76-78 solute carrier family 6 member 3 Homo sapiens 103-123 10037481-2 1999 This was addressed here by studying the inhibition by dopamine of the binding of [3H]WIN 35,428 [2beta-carbomethoxy-3beta-(4-fluorophenyl)[3H]tropane], a phenyltropane analogue of cocaine, to the cloned human dopamine transporter expressed in HEK-293 cells. Tritium 82-84 solute carrier family 6 member 3 Homo sapiens 209-229 10215516-7 1999 A decrease in the total binding sites (Bmax) of [3H]PDBu binding was only observed in membrane PKC. Tritium 49-51 protein kinase C, gamma Rattus norvegicus 95-98 9600933-4 1998 VZG-1 expression was necessary and sufficient in mediating multiple effects of LPA: [3H]-LPA binding, G protein activation, stress fiber formation, neurite retraction, serum response element activation, and increased DNA synthesis. Tritium 85-87 lysophosphatidic acid receptor 1 Homo sapiens 0-5 9633834-0 1998 Neuropeptide Y-mediated enhancement of NMDA-stimulated [3H]dopamine release from rat prefrontal cortex is reversed by sigma1 receptor antagonists. Tritium 56-58 sigma non-opioid intracellular receptor 1 Rattus norvegicus 118-133 9602106-2 1998 [3H]citalopram binding, reflecting the presence of 5-HT transporter on thalamocortical fibers, produced a clearcut barrel pattern from P4 to P16 (peak at P8), and decreased to a dispersed, low density in the adult. Tritium 1-3 cyclin-dependent kinase inhibitor 2A Rattus norvegicus 141-144 9730266-2 1998 The pharmacological profile and the anatomical localization of a putative dopamine D4 receptor were assessed in sections of rat and human atria and ventricles using combined radioligand binding and autoradiographic techniques with [3H]-spiperone as a ligand. Tritium 232-234 dopamine receptor D4 Rattus norvegicus 74-94 9606055-4 1998 Kinetic analysis revealed that association of specific [3H]urocortin binding was monophasic and slow and that the binding was irreversible. Tritium 56-58 urocortin Rattus norvegicus 59-68 9606055-6 1998 The apparent Kd for [3H]urocortin binding of 0.25 nM is similar to previously reported affinities of rat urocortin for hCRF-BP. Tritium 21-23 urocortin Rattus norvegicus 24-33 9489732-6 1998 Simultaneous analysis with the agonists [3H]clonidine and [3H]UK14304 and the antagonist [3H]RX821002 in the same depressed suicides confirmed the enhanced alpha2A-adrenoceptor density when evaluated by agonist, but not by antagonist, radioligands. Tritium 41-43 adrenoceptor alpha 2A Homo sapiens 156-176 9489732-6 1998 Simultaneous analysis with the agonists [3H]clonidine and [3H]UK14304 and the antagonist [3H]RX821002 in the same depressed suicides confirmed the enhanced alpha2A-adrenoceptor density when evaluated by agonist, but not by antagonist, radioligands. Tritium 59-61 adrenoceptor alpha 2A Homo sapiens 156-176 9489732-6 1998 Simultaneous analysis with the agonists [3H]clonidine and [3H]UK14304 and the antagonist [3H]RX821002 in the same depressed suicides confirmed the enhanced alpha2A-adrenoceptor density when evaluated by agonist, but not by antagonist, radioligands. Tritium 59-61 adrenoceptor alpha 2A Homo sapiens 156-176 10029091-8 1999 Finally, bombesin significantly increased [3H]thymidine uptake by Isreco1 cells in a dose-dependent manner, with a first significant response at 0.1 nM and a maximal effect at 100 nM bombesin (192.2+/-9.7% of control). Tritium 43-45 gastrin releasing peptide Homo sapiens 9-17 10029091-8 1999 Finally, bombesin significantly increased [3H]thymidine uptake by Isreco1 cells in a dose-dependent manner, with a first significant response at 0.1 nM and a maximal effect at 100 nM bombesin (192.2+/-9.7% of control). Tritium 43-45 gastrin releasing peptide Homo sapiens 183-191 10049740-5 1999 In acute experiments, equilibrium binding with [3H]-CGP-12177 to beta-AR was not directly inhibited by Am and the equilibrium binding constant did not change during prolonged exposure up to 72 hours. Tritium 48-50 adrenergic receptor, beta 1 Mus musculus 65-72 9730919-3 1998 Here, we compare the drug binding properties of SIs and related proteins with [3H]ifenprodil as a common high affinity radioligand (Kd = 1.4-19 nM), demonstrating an intimate pharmacological relationship among ERG2p, sigma1 receptor, and EBP. Tritium 79-81 potassium voltage-gated channel subfamily H member 6 Homo sapiens 210-215 10512568-5 1999 Neurochemical studies of brains removed after the Morris water maze task showed that choline acetyltransferase activity in the cerebral cortex, but not the hippocampus, was significantly reduced by NBM lesioning, while there were no changes of muscarinic M1 receptor binding activity detected using [3H]pirenzepine. Tritium 300-302 choline O-acetyltransferase Rattus norvegicus 85-110 9772093-2 1998 In addition, light microscopic autoradiography was used to localize sites of metabolic activation of 3H-labeled Trp-P-1 (3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole), a heterocyclic amine known to be metabolized by CYP1A1, in rodent tissue slices. Tritium 101-103 cytochrome P450, family 1, subfamily a, polypeptide 1 Rattus norvegicus 215-221 10036221-4 1999 Cytosols from liver and lung of the sensitive L-E rats contained about twofold higher levels of specific binding sites for [3H]TCDD than occurred in H/W rats; the Kd for binding of [3H]TCDD to AHR in hepatic cytosols was similar between the two strains. Tritium 124-126 aryl hydrocarbon receptor Rattus norvegicus 193-196 10036221-4 1999 Cytosols from liver and lung of the sensitive L-E rats contained about twofold higher levels of specific binding sites for [3H]TCDD than occurred in H/W rats; the Kd for binding of [3H]TCDD to AHR in hepatic cytosols was similar between the two strains. Tritium 182-184 aryl hydrocarbon receptor Rattus norvegicus 193-196 9989560-3 1999 METHOD: In the present study, binding of the cocaine analog [3H]WIN 35428 to the dopamine transporter was assayed in postmortem striatal samples from 15 cocaine-using subjects and 15 matched comparison subjects to determine whether there were differences in number of binding sites or in affinity. Tritium 61-63 solute carrier family 6 member 3 Homo sapiens 81-101 10374635-4 1998 In basal state and Ang-stimulated state, [3H]thymidine incorporation was inhibited by 26.5% (P < 0.01) and 42.0% (P < 0.01) and cell number was inhibited by 17.3% (P < 0.01) and by 22.2% (P < 0.01), respectively. Tritium 42-44 angiogenin Rattus norvegicus 19-22 9452451-3 1998 [3H]Retinol transfer from RBP to CRBP-Strep was monitored by measuring the radioactivity associated with CRBP-Strep retained by an immobilized streptavidin resin. Tritium 1-3 retinol binding protein 4 Homo sapiens 26-29 9639282-7 1998 [3H]D-Ala2,N-Me-Phe4,Gly-ol5-enkephalin binding to mu opioid receptors was significantly higher in the globus pallidus, amygdala, thalamus, hypothalamus, hippocampus, substantia nigra, ventral tegmental area, central gray, and interpeduncular nucleus of the naltrexone-treated rats. Tritium 1-3 proenkephalin Rattus norvegicus 29-39 9453571-5 1998 (-)-Nicotine exposure from P1 to P21 significantly up-regulated the number of [3H]epibatidine and high-affinity (-)-[3H]nicotine binding sites in most of the brain regions studied but did not influence the number of [3H]alpha-Bgt binding sites. Tritium 79-81 cyclin-dependent kinase inhibitor 1A (P21) Mus musculus 33-36 9453571-5 1998 (-)-Nicotine exposure from P1 to P21 significantly up-regulated the number of [3H]epibatidine and high-affinity (-)-[3H]nicotine binding sites in most of the brain regions studied but did not influence the number of [3H]alpha-Bgt binding sites. Tritium 117-119 cyclin-dependent kinase inhibitor 1A (P21) Mus musculus 33-36 9453571-5 1998 (-)-Nicotine exposure from P1 to P21 significantly up-regulated the number of [3H]epibatidine and high-affinity (-)-[3H]nicotine binding sites in most of the brain regions studied but did not influence the number of [3H]alpha-Bgt binding sites. Tritium 117-119 cyclin-dependent kinase inhibitor 1A (P21) Mus musculus 33-36 9453571-7 1998 (-)-Nicotine exposure during P8 to P16 resulted in a significant and long-lasting increase in the number of nAChR isoforms labeled by (-)-[3H]nicotine, but not by [3H]epibatidine, in the cortex, hippocampus, and striatum of adult rat. Tritium 139-141 cyclin-dependent kinase inhibitor 2A Rattus norvegicus 35-38 9453571-7 1998 (-)-Nicotine exposure during P8 to P16 resulted in a significant and long-lasting increase in the number of nAChR isoforms labeled by (-)-[3H]nicotine, but not by [3H]epibatidine, in the cortex, hippocampus, and striatum of adult rat. Tritium 139-141 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 108-113 9492296-1 1998 The O-linked sugar chains of the human leukocyte cell surface glycoprotein CD45 were released as tritium-labeled oligosaccharides by beta-elimination in the presence of NaB3H4. Tritium 97-104 protein tyrosine phosphatase receptor type C Homo sapiens 75-79 10024333-7 1999 Antibodies against alpha1A-, alpha1B-, and alpha1D-receptor subtypes decreased [3H]prazosin binding to a different extent. Tritium 80-82 calcium voltage-gated channel subunit alpha1 A Homo sapiens 19-26 9916092-6 1999 The ADP-ribosyltransferase-deficient CT-2* protein, which was incapable of increasing synthesis of cAMP, displayed about the same capacity as CT to evoke the release of [3H]AA metabolites from both S49 WT and cyc- cells. Tritium 170-172 cardiotrophin 2 Mus musculus 37-41 9916092-9 1999 In addition, the B subunits purified from native CT and CT-2* both simulated the release of [3H]AA from S49 cyc- cells and murine monocyte/macrophage cells (RAW 264.7), suggesting a receptor-mediated cell activation process of potential importance in enhancing immune responses to vaccine components. Tritium 93-95 cardiotrophin 2 Mus musculus 56-60 9930760-2 1999 Very high relative densities of [3H]L-AP4 binding were observed in the molecular layer of the cerebellar cortex, the nucleus basalis, the outer layer of the superior colliculus, and the substantia nigra. Tritium 33-35 transcription factor AP4 Mus musculus 38-41 9930760-6 1999 In these regions, mGluR8 is likely to be labeled by [3H]L-AP4 because mGluR8 is expressed in such brain regions and, like mGluR4, has high affinity for L-AP4. Tritium 53-55 transcription factor AP4 Mus musculus 58-61 9930760-6 1999 In these regions, mGluR8 is likely to be labeled by [3H]L-AP4 because mGluR8 is expressed in such brain regions and, like mGluR4, has high affinity for L-AP4. Tritium 53-55 transcription factor AP4 Mus musculus 154-157 9930760-7 1999 We conclude that mGluR4 contributes substantially to the high-affinity binding site for [3H]L-AP4 in several regions of mouse brain, including cerebellar cortex, nucleus basalis, thalamus, superior colliculus, substantia nigra, and hippocampal dentate gyrus. Tritium 89-91 transcription factor AP4 Mus musculus 94-97 9489623-4 1998 2 Expression of type II adenylyl cyclase resulted in a substantially elevated capacity of isoprenaline to stimulate [3H]-forskolin binding, whereas in CAM beta2-adrenoceptor expressing cells the basal high affinity [3H]-forskolin binding represented a markedly greater % of the maximal effect which could be produced by addition of isoprenaline, and the EC50 for isoprenaline was some 10 fold lower than in cells expressing the wild type beta2-adrenoceptor. Tritium 216-218 adrenoceptor beta 2 Homo sapiens 155-173 9453002-5 1998 Endothelin-1-stimulated [3H]-thymidine incorporation, CDK4 kinase activity, and the percent of cells in S phase were found to be significantly inhibited by overexpression of p16INK4 and slightly inhibited by overexpression of p21cip1. Tritium 25-27 cyclin-dependent kinase inhibitor 2A Rattus norvegicus 174-181 10073755-5 1999 Using an in vitro 3H-labeled elastin degradation assay, elastin-degrading enzyme activity was only observed in the bacteria-conditioned medium from an isolate of Pseudomonas aeruginosa. Tritium 18-20 elastin Homo sapiens 29-36 9660757-5 1998 Overexpression of Galphaq with PLCbeta3 or PLCbeta (Ser1105--> Ala) mutant in COSM6 cells resulted in a 5-fold increase in [3H]phosphatidylinositol 1,4,5-trisphosphate formation compared with expression of Galphaq, PLCbeta3, or PLCbeta3 (Ser1105 --> Ala mutant alone. Tritium 127-129 G protein subunit alpha q Homo sapiens 18-25 10073755-5 1999 Using an in vitro 3H-labeled elastin degradation assay, elastin-degrading enzyme activity was only observed in the bacteria-conditioned medium from an isolate of Pseudomonas aeruginosa. Tritium 18-20 elastin Homo sapiens 56-63 9862783-2 1999 The IC50 for inhibiting [3H]LTB4 binding to human neutrophils was 17.6 +/- 4.8 nM. Tritium 25-27 prostaglandin reductase 1 Cavia porcellus 28-32 9585173-3 1998 Lafutidine enhanced [3H]glucosamine incorporation into the mucin in the full thickness corpus mucosa, but not in the antrum. Tritium 21-23 solute carrier family 13 member 2 Rattus norvegicus 59-64 9671776-2 1998 Leptin, PPARalpha/retinoid x receptor ligands, and FA all up-regulate PPARalpha and enzymes of FA oxidation and stimulate [3H]-palmitate oxidation in normal islets but not in islets from fa/fa rats. Tritium 123-125 peroxisome proliferator activated receptor alpha Rattus norvegicus 8-17 9585173-5 1998 The lafutidine-induced increase of [3H]-labeled mucin in the corpus was completely blocked by either NG-nitro-L-arginine (10[-5] M) or 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazolne-1-oxyl-3-oxide (10[-5] M). Tritium 36-38 solute carrier family 13 member 2 Rattus norvegicus 48-53 9889056-9 1999 Each reaction contained substrate as [3H]glucosamine-labeled HSPG (>50 kDa) from the BM of the EHS tumor, CM from PC-3M or LNCaP C4-2 cells, and inhibitor or buffer (negative control). Tritium 38-40 syndecan 2 Homo sapiens 61-65 9836514-6 1998 Moreover, radiolabeled [3H]KRP-297 bound directly to PPAR-alpha and PPAR-gamma with dissociation constants of 228 and 326 nmol/l, respectively. Tritium 24-26 peroxisome proliferator-activated receptor gamma Rattus norvegicus 68-78 9698074-3 1998 The purpose of this study was to partially characterize the incorporation of [3H]VPA into phospholipids of GT1-7 neurons, an immortalized hypothalamic cell line. Tritium 78-80 beta-1,4-galactosyltransferase 1 Homo sapiens 107-110 9513188-6 1998 Mitochondrial benzodiazepine receptor pharmacology was studied using [3H] PK11195 binding to cancer cell homogenates and isolated kidney mitochondrial membranes. Tritium 70-72 translocator protein Homo sapiens 0-37 9698074-6 1998 Our results indicate that [3H]VPA (10 microg/mL) was incorporated into phospholipids of GT1-7 neurons in a time-dependent and saturable manner over 300 min. Tritium 27-29 beta-1,4-galactosyltransferase 1 Homo sapiens 88-91 9459575-2 1998 (1) Exposure to CNP (10 nM-1 microM for 72 h) inhibited [3H]thymidine incorporation into mesangial cells in a concentration-dependent manner. Tritium 57-59 natriuretic peptide C Rattus norvegicus 16-19 9459575-3 1998 Atrial natriuretic peptide (1 nM-1 microM), a peptide related to CNP, also decreased [3H]thymidine incorporation into these cells in a concentration-dependent manner. Tritium 86-88 natriuretic peptide C Rattus norvegicus 65-68 10089064-10 1998 The VP-16 induced increment of [3H]-L-leucine uptake was canceled by addition of 100 ng/ml of ACR. Tritium 32-34 host cell factor C1 Homo sapiens 4-9 9675089-2 1998 [3H]MEN 11420 bound to a single class of high affinity binding sites: its binding was inhibited by natural tachykinins (potency ranking: NKA >> SP > or = NKB), as well as by peptide (MEN 11420 > MEN 10376 >> R 396) and nonpeptide (SR 48968 > GR 159897) selective NK2 receptor antagonists. Tritium 1-3 tachykinin precursor 3 Homo sapiens 163-166 9864263-1 1998 (+/-)-Epibatidine (EPIB) and A-85380 are nicotinic acetylcholine receptor (nAChR) agonists that bind to the agonist ([3H]cytisine) binding site with 40 to 50 pM affinity but have different affinities in nAChR subtype selective functional receptor assays. Tritium 118-120 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 41-73 9864263-1 1998 (+/-)-Epibatidine (EPIB) and A-85380 are nicotinic acetylcholine receptor (nAChR) agonists that bind to the agonist ([3H]cytisine) binding site with 40 to 50 pM affinity but have different affinities in nAChR subtype selective functional receptor assays. Tritium 118-120 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 75-80 9864263-1 1998 (+/-)-Epibatidine (EPIB) and A-85380 are nicotinic acetylcholine receptor (nAChR) agonists that bind to the agonist ([3H]cytisine) binding site with 40 to 50 pM affinity but have different affinities in nAChR subtype selective functional receptor assays. Tritium 118-120 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 203-208 9459575-5 1998 (3) The cyclic GMP analog, 8-bromo-cyclic GMP (100 microM and 1 microM), mimicked the inhibitory effects of CNP and atrial natriuretic peptide on [3H]thymidine incorporation into mesangial cells, whereas inhibitors of protein kinase C, protein kinase A, and protein kinase G reduced the effect of both natriuretic peptides. Tritium 147-149 natriuretic peptide C Rattus norvegicus 108-111 9459575-7 1998 (4) CNP and atrial natriuretic peptide decreased interleukin-1-, interleukin-6-, platelet derived growth factor-, angiotensin II-induced [3H]thymidine incorporation into mesangial cells. Tritium 138-140 natriuretic peptide C Rattus norvegicus 4-7 9681926-7 1998 Inhibitor constants (Ki) obtained for L-HCSA, L-CSA, L-CA and L-SOS in [3H]glutamate receptor binding studies with mGluR1alpha cells indicated that L-HCSA, L-CSA, L-CA and L-SOS can bind specifically to mGluR1 with L-HCSA showing the highest affinity. Tritium 72-74 protein tyrosine phosphatase receptor type C Homo sapiens 53-57 9421306-11 1997 NPY, PYY and PYY(3-36) inhibited [3H]-noradrenaline release from preparations of epididymis (pIC50 values 7.9+/-0.7, 9.6+/-0.8 and 10.0+/-0.9, respectively, all n=6). Tritium 34-36 pro-neuropeptide Y Cavia porcellus 0-3 9353280-11 1997 The binding of [3H]PIP2 was saturable and could be competitively decreased by addition of unlabeled PIP2. Tritium 16-18 plasma membrane intrinsic protein 2A Arabidopsis thaliana 19-23 9353280-11 1997 The binding of [3H]PIP2 was saturable and could be competitively decreased by addition of unlabeled PIP2. Tritium 16-18 plasma membrane intrinsic protein 2A Arabidopsis thaliana 100-104 9401759-4 1997 McN-A-343 and AHR-602, as well as carbachol, completely displaced the atropine-sensitive binding of [3H]-quinuclidinyl benzilate to muscarinic receptors present in the membrane preparation. Tritium 101-103 aryl hydrocarbon receptor Cavia porcellus 14-17 9811854-2 1998 A dramatic overall reduction of protein prenylation was found in v-K-ras-transformed cells in comparison with the parent FRTL-5 cells, as shown by labeling cells with [3H]mevalonic acid. Tritium 168-170 KRAS proto-oncogene, GTPase Rattus norvegicus 67-72 9811854-6 1998 The K-ras oncogene activation also resulted in an overall decrease of [3H]-MVA incorporation into isopentenyl-tRNA together with an increase of unprocessed [3H]-MVA and no alteration in [3H]-MVA uptake. Tritium 71-73 KRAS proto-oncogene, GTPase Rattus norvegicus 4-9 9811854-6 1998 The K-ras oncogene activation also resulted in an overall decrease of [3H]-MVA incorporation into isopentenyl-tRNA together with an increase of unprocessed [3H]-MVA and no alteration in [3H]-MVA uptake. Tritium 157-159 KRAS proto-oncogene, GTPase Rattus norvegicus 4-9 9811854-6 1998 The K-ras oncogene activation also resulted in an overall decrease of [3H]-MVA incorporation into isopentenyl-tRNA together with an increase of unprocessed [3H]-MVA and no alteration in [3H]-MVA uptake. Tritium 157-159 KRAS proto-oncogene, GTPase Rattus norvegicus 4-9 9811854-7 1998 The effects of v-K-ras on protein prenylation could be mimicked in FRTL-5 cells by lowering the concentration of exogenous [3H]-MVA whereas increasing the [3H]-MVA concentration did not revert the alterations observed in transformed cells. Tritium 124-126 KRAS proto-oncogene, GTPase Rattus norvegicus 17-22 9347319-4 1997 A series of kinetic studies of [3H]8-OH-DPAT binding to the transfected HA6 cell line demonstrated two components in both the association and the dissociation reactions. Tritium 32-34 keratin 36 Homo sapiens 72-75 9618152-5 1998 Even at very low doses (0.01 U/ml), IFNgamma significantly inhibited IEC-6 cell proliferation, as demonstrated by reduced 3H-thymidine uptake, stable cell count, and complete arrest in the quiescent G0/G1 phase of the cell cycle. Tritium 122-124 interferon gamma Rattus norvegicus 36-44 9421172-5 1997 There was a strong positive correlation between the ratio of secreted APP to membrane-bound APP and the activity of choline acetyltransferase and M1 muscarinic acetylcholine receptor density (measured by [3H]pirenzepine binding) in experimental groups. Tritium 205-207 choline O-acetyltransferase Rattus norvegicus 116-141 9806335-17 1998 Kazinol B partially inhibited the [3H]phorbol 12,13-dibutyrate ([3H]PDB) binding to the neutrophil cytosolic PKC. Tritium 35-37 protein kinase C, alpha Rattus norvegicus 109-112 9806335-17 1998 Kazinol B partially inhibited the [3H]phorbol 12,13-dibutyrate ([3H]PDB) binding to the neutrophil cytosolic PKC. Tritium 65-67 protein kinase C, alpha Rattus norvegicus 109-112 9794249-1 1998 Multiple affinity states are revealed by agonist competition for radioantagonist [3H]SR141716A binding to rat brain CB1 cannabinoid receptors. Tritium 82-84 cannabinoid receptor 1 Rattus norvegicus 116-119 9632674-7 1998 Of significance, oatp1-mediated taurocholate and LTC4 uptake was cis-inhibited and trans-stimulated by GSH, and [3H]GSH efflux was enhanced in the presence of extracellular taurocholate or sulfobromophthalein, indicating that GSH efflux down its large electrochemical gradient provides the driving force for uptake via oatp1. Tritium 113-115 solute carrier organic anion transporter family member 4A1 L homeolog Xenopus laevis 17-22 9728768-8 1998 The fragment pattern of the [35S]methionine- or [3H]leucine-labeled polypeptides revealed that EMR3 and EMR4 were exposed with opposite orientation either on the cytosolic or luminal side of the ER membrane supporting the 4-transmembrane helix (TMH) N(in) model with the N and C termini on the cytoplasmic side, as established for the myelin membrane (plasma membrane); the other inversely integrated PLP constructs indicate the 4-TMH-Nout profile. Tritium 49-51 adhesion G protein-coupled receptor E4, pseudogene Homo sapiens 104-108 9804290-3 1998 caused significant decreases in [3H]MK801 binding in several hippocampus subareas and layers, mainly in CA1 and CA3 at seizure (11-27%) and postseizure (8-16%) and in cerebral occipital cortex at seizure (18-22%). Tritium 33-35 carbonic anhydrase 3 Rattus norvegicus 112-115 9390408-0 1997 Tritium NMR studies of the human carbonic anhydrase I-benzenesulfonamide complex. Tritium 0-7 carbonic anhydrase 1 Homo sapiens 33-53 9390408-1 1997 Tritium NMR spectroscopy has been used to examine the complex formed by [4-3H]benzenesulfon-amide and human carbonic anhydrase I. Tritium 0-7 carbonic anhydrase 1 Homo sapiens 108-128 9369329-5 1997 [3H]Flunitrazepam binding was enhanced by 3alpha-OH-DHP in every nucleus examined in the medulla and cerebellum. Tritium 1-3 dihydropyrimidinase Rattus norvegicus 52-55 9845009-2 1998 NT(1-13) and NT(8-13) increased (3H)5HT release with EC50 values in the nanomolar range and Emax values in the range of 100% of control, whereas D-tyr11-NT was inactive. Tritium 33-35 neurotensin Rattus norvegicus 0-2 9696056-2 1998 Binding of [3H]nociceptin was quantified in spinal segment L4 of rats at 2, 4 and 8 days following injection of complete Freund"s adjuvant (CFA) into one hind-paw. Tritium 12-14 prepronociceptin Rattus norvegicus 15-25 9845009-2 1998 NT(1-13) and NT(8-13) increased (3H)5HT release with EC50 values in the nanomolar range and Emax values in the range of 100% of control, whereas D-tyr11-NT was inactive. Tritium 33-35 neurotensin Rattus norvegicus 13-15 9845009-2 1998 NT(1-13) and NT(8-13) increased (3H)5HT release with EC50 values in the nanomolar range and Emax values in the range of 100% of control, whereas D-tyr11-NT was inactive. Tritium 33-35 neurotensin Rattus norvegicus 13-15 9845009-3 1998 Concerning NT receptor antagonists, SR 48692 and SR 142948A antagonized with IC50 values of 4.8+/-1.8 nM and 4.5+/-1.8 nM respectively, the NT stimulated K+-evoked (3H)5HT release. Tritium 165-167 neurotensin Rattus norvegicus 140-142 9696056-3 1998 Densitometric analysis of autoradiograms showed that [3H]nociceptin binding increased in medial and lateral laminae I-II bilaterally 4 days following injection of CFA compared to untreated rats; no change in binding occurred in lamina X at the times examined. Tritium 54-56 prepronociceptin Rattus norvegicus 57-67 9774213-5 1998 Using transfected cells expressing either 5-HT1B or 5-HT1D receptors, it was shown that 5-HT-moduline prevents the binding of [3H]5-HT to 5-HT1B as well as to 5-HT1D receptors with similar biochemical characteristics: the IC50 of the peptide was 1.2x10(-12) M for 5-HT1B and 9x10(-13) M for 5-HT1D receptors. Tritium 127-129 5-hydroxytryptamine receptor 1D Homo sapiens 52-58 9774213-5 1998 Using transfected cells expressing either 5-HT1B or 5-HT1D receptors, it was shown that 5-HT-moduline prevents the binding of [3H]5-HT to 5-HT1B as well as to 5-HT1D receptors with similar biochemical characteristics: the IC50 of the peptide was 1.2x10(-12) M for 5-HT1B and 9x10(-13) M for 5-HT1D receptors. Tritium 127-129 5-hydroxytryptamine receptor 1D Homo sapiens 159-165 9774213-5 1998 Using transfected cells expressing either 5-HT1B or 5-HT1D receptors, it was shown that 5-HT-moduline prevents the binding of [3H]5-HT to 5-HT1B as well as to 5-HT1D receptors with similar biochemical characteristics: the IC50 of the peptide was 1.2x10(-12) M for 5-HT1B and 9x10(-13) M for 5-HT1D receptors. Tritium 127-129 5-hydroxytryptamine receptor 1D Homo sapiens 159-165 9664843-6 1998 These mode matches successfully predicted the nonlinear kinetic interactions of NT-(DA)D2 in contrast with CCK-(DA) D2 on 3H-spiperone binding to (DA) D2, and by CCK-DAT but not NT-DAT on [N-methyl-3H]-WIN 35,428 binding to DAT in (DA)D2 and DAT cDNA stably transfected cell lines without known NT or CCK receptors. Tritium 122-124 solute carrier family 6 member 3 Homo sapiens 181-184 9303569-14 1997 Finally, the addition of 100 microM GTP reduced the binding of [3H]-GR-125743 to h5-HT1D and h5-HT1B receptor subtypes by approximately 20% without affecting the affinities obtained for different displacers. Tritium 64-66 5-hydroxytryptamine receptor 1D Homo sapiens 82-88 9400680-5 1997 First, HGF induces a 45% increase in [3H]thymidine incorporation and a 65% increase in cell number by crystal violet analysis at 10 ng/mL concentration, and the increase is dose dependent. Tritium 38-40 hepatocyte growth factor Mus musculus 7-10 9260982-5 1997 In adult myocytes (cultured without bromodeoxyuridine), Ang II stimulated [3H]leucine incorporation by 24+/-2.3% over control; with bromodeoxyuridine, that stimulation was reduced significantly (13+/-0.93% over control). Tritium 75-77 angiogenin Rattus norvegicus 56-59 9664843-6 1998 These mode matches successfully predicted the nonlinear kinetic interactions of NT-(DA)D2 in contrast with CCK-(DA) D2 on 3H-spiperone binding to (DA) D2, and by CCK-DAT but not NT-DAT on [N-methyl-3H]-WIN 35,428 binding to DAT in (DA)D2 and DAT cDNA stably transfected cell lines without known NT or CCK receptors. Tritium 122-124 solute carrier family 6 member 3 Homo sapiens 181-184 9696056-4 1998 Biochemical studies confirmed that the specific binding of [3H]nociceptin to sections of rat brain was consistent with the binding characteristics of the nociceptin receptor. Tritium 59-62 prepronociceptin Rattus norvegicus 63-73 9664843-6 1998 These mode matches successfully predicted the nonlinear kinetic interactions of NT-(DA)D2 in contrast with CCK-(DA) D2 on 3H-spiperone binding to (DA) D2, and by CCK-DAT but not NT-DAT on [N-methyl-3H]-WIN 35,428 binding to DAT in (DA)D2 and DAT cDNA stably transfected cell lines without known NT or CCK receptors. Tritium 122-124 solute carrier family 6 member 3 Homo sapiens 181-184 9262359-2 1997 In the present study, we address this issue by examining the inhibition by mazindol of the binding of [3H]WIN 35,428 ([3H]2beta-carbomethyoxy-3beta-(4-fluorophenyl)-tropane), a phenyltropane analog of cocaine, and the inhibition by WIN 35,428 of [3H]mazindol binding to the cloned human dopamine transporter expressed in C6 glioma cells. Tritium 103-105 solute carrier family 6 member 3 Homo sapiens 287-307 9637489-1 1998 Our previous study showed that CD9 costimulation of TCR-triggered naive T cells elicits activation ([3H]TdR incorporation) that is similar to CD28 costimulation; however, unlike CD28 costimulation, CD9 costimulation results in apoptosis of these previously activated T cells. Tritium 101-103 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 52-55 9220462-1 1997 When rat cultured neurons were incubated with unlabelled neurotensin (3 nM) for 1 or 24 h at 37 degrees C, the [3H]-neurotensin specific binding measured in cell homogenates was decreased to about 35 and 65% of control values, respectively. Tritium 112-114 neurotensin Rattus norvegicus 57-68 9220462-1 1997 When rat cultured neurons were incubated with unlabelled neurotensin (3 nM) for 1 or 24 h at 37 degrees C, the [3H]-neurotensin specific binding measured in cell homogenates was decreased to about 35 and 65% of control values, respectively. Tritium 112-114 neurotensin Rattus norvegicus 116-127 9694942-3 1998 Determination of high-affinity [3H]forskolin binding to intact cells, which provides a direct parameter for the binding of the activated alpha-subunit of Gs (Gsalpha) to AC, revealed that the enhancement of AC activity after opioid withdrawal is not caused by an increased stimulation of effector activity by Gsalpha. Tritium 32-34 GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus Mus musculus 158-165 9674635-3 1998 In VSMCs, both E2 and DHT had a biphasic effect on [3H]thymidine incorporation into DNA: low concentrations (0.3 nmol/L for E2, 3 nmol/L for DHT) stimulated [3H]thymidine incorporation (+35% and +41%, respectively), whereas high concentrations (30 nmol/L for E2, 300 nmol/L for DHT) inhibited [3H]thymidine incorporation (-40%). Tritium 52-54 cystatin 12, pseudogene Homo sapiens 15-25 9259019-6 1997 In contrast, lower net or percent increases (p < 0.025) in GABA-enhanced [3H]FNZ binding were found in the entorhinal cortex, the mediodorsal thalamus, and the dorsal CA3 area and middle dentate gyrus of the posterior hippocampus of the P line relative to the NP line. Tritium 77-79 carbonic anhydrase 3 Rattus norvegicus 170-173 9674635-3 1998 In VSMCs, both E2 and DHT had a biphasic effect on [3H]thymidine incorporation into DNA: low concentrations (0.3 nmol/L for E2, 3 nmol/L for DHT) stimulated [3H]thymidine incorporation (+35% and +41%, respectively), whereas high concentrations (30 nmol/L for E2, 300 nmol/L for DHT) inhibited [3H]thymidine incorporation (-40%). Tritium 158-160 cystatin 12, pseudogene Homo sapiens 15-25 9614205-5 1998 Incubation of cells with AO for 48 hr decreased alpha1B-adrenoceptor density ([3H]prazosin Bmax) versus control groups by 12% (1 microM AO) and 72% (10 microM AO). Tritium 79-81 alpha-1B adrenergic receptor Mesocricetus auratus 48-68 9674635-3 1998 In VSMCs, both E2 and DHT had a biphasic effect on [3H]thymidine incorporation into DNA: low concentrations (0.3 nmol/L for E2, 3 nmol/L for DHT) stimulated [3H]thymidine incorporation (+35% and +41%, respectively), whereas high concentrations (30 nmol/L for E2, 300 nmol/L for DHT) inhibited [3H]thymidine incorporation (-40%). Tritium 158-160 cystatin 12, pseudogene Homo sapiens 15-25 9224784-4 1997 SDS-PAGE and Western blot analysis of UGT1.1-transfected HK293 membrane proteins photolabeled with [11,12-3H]atRA revealed a protein of approximately 56 kDa that was labeled by [3H]atRA, detected by anti-pNP UGT antibody and not present in membranes from nontransfected HK293 cells. Tritium 106-108 UDP glucuronosyltransferase family 1 member A1 Homo sapiens 38-44 9702002-8 1998 Difluoromethylornithine, a specific inhibitor of ODC, decreased the level of PUT in the liver, and inhibited [3H] thymidine incorporation. Tritium 110-112 ornithine decarboxylase 1 Rattus norvegicus 49-52 9199198-4 1997 Thrombin caused a 3-4-fold increase in [3H]thymidine incorporation within 24 h which was prevented by inhibitors of NFkappaB activation. Tritium 40-42 coagulation factor II, thrombin Bos taurus 0-8 9220028-3 1997 Stimulation of the glioblastoma cell line U-373MG with bombesin or gastrin-releasing peptide (GRP) induced mitogenesis, measured by [3H]thymidine incorporation into DNA, and stimulated the tyrosine phosphorylation of the mitogen-activated protein (MAP) kinases (Erk1 and Erk2). Tritium 133-135 gastrin releasing peptide Homo sapiens 55-63 9220028-3 1997 Stimulation of the glioblastoma cell line U-373MG with bombesin or gastrin-releasing peptide (GRP) induced mitogenesis, measured by [3H]thymidine incorporation into DNA, and stimulated the tyrosine phosphorylation of the mitogen-activated protein (MAP) kinases (Erk1 and Erk2). Tritium 133-135 gastrin releasing peptide Homo sapiens 67-92 9220028-3 1997 Stimulation of the glioblastoma cell line U-373MG with bombesin or gastrin-releasing peptide (GRP) induced mitogenesis, measured by [3H]thymidine incorporation into DNA, and stimulated the tyrosine phosphorylation of the mitogen-activated protein (MAP) kinases (Erk1 and Erk2). Tritium 133-135 gastrin releasing peptide Homo sapiens 94-97 9182580-7 1997 200 nM fibrinogen induced a 3-fold increase in [3H]thymidine incorporation by 293 cells transfected with ICAM-1 cDNA but not control non-transfected 293 cells. Tritium 48-50 intercellular adhesion molecule 1 Homo sapiens 105-111 9436464-3 1997 Anti-CD9 or anti-CD28 monoclonal antibody (mAb) increased [3H]TdR incorporation of naive T-cells in the absence of antigen-presenting cells (APC) when coimmobilized with submitogenic doses of anti-CD3 mAb. Tritium 59-61 CD9 molecule Homo sapiens 5-8 9436464-8 1997 The combined costimulation of anti-CD3-triggered T-cells with anti-CD9 and anti-CD28 mAbs resulted in strikingly enhanced [3H]TdR uptake and lymphokine (IL-2 and IFN-gamma) production when compared to those induced by each costimulation. Tritium 123-125 CD9 molecule Homo sapiens 67-70 9609761-4 1998 Expression of IL-15 mRNA was analyzed by in situ hybridization, and proliferative response of LPMCs to recombinant IL-15 was determined by [3H]thymidine incorporation into DNA. Tritium 140-142 interleukin 15 Homo sapiens 115-120 9643362-2 1998 FRTL5 cells incorporated [3H]PAF and deacetylated this compound to the corresponding [3H]lyso-PAF which was not accumulated or secreted but converted mainly to alkyl-acyl-phosphocholine indicating that this acylation process was particularly active in these cells. Tritium 26-28 PCNA clamp associated factor Rattus norvegicus 29-32 9643362-2 1998 FRTL5 cells incorporated [3H]PAF and deacetylated this compound to the corresponding [3H]lyso-PAF which was not accumulated or secreted but converted mainly to alkyl-acyl-phosphocholine indicating that this acylation process was particularly active in these cells. Tritium 86-88 PCNA clamp associated factor Rattus norvegicus 29-32 9643362-2 1998 FRTL5 cells incorporated [3H]PAF and deacetylated this compound to the corresponding [3H]lyso-PAF which was not accumulated or secreted but converted mainly to alkyl-acyl-phosphocholine indicating that this acylation process was particularly active in these cells. Tritium 86-88 PCNA clamp associated factor Rattus norvegicus 94-97 9606532-5 1998 Kinetic binding properties of pBR were measured using 3H-PK11195 as a ligand. Tritium 54-56 translocator protein Homo sapiens 30-33 11540567-3 1997 The ability of bone marrow cells to respond to GM-CSF was measured by 3H-TdR incorporation method. Tritium 70-72 colony stimulating factor 2 Rattus norvegicus 47-53 9631438-1 1998 The selectivity of the muscarinic toxin MT3 from green mamba snake venom was corroborated by inhibition of the binding of [3H]NMS, a classical muscarinic radioligand, to native and cloned muscarinic receptors, showing 214-fold higher affinity for m4 than for m1 subtype, without significant binding to the others. Tritium 123-125 metallothionein 3 Rattus norvegicus 40-43 9241757-7 1997 In cultured bovine coronary smooth muscle cells, the mitogenic activity of thrombin (3 nM), measured by [3H]thymidine incorporation, was also suppressed by triabin. Tritium 105-107 coagulation factor II, thrombin Bos taurus 75-83 9578599-6 1998 In contrast to previous reports, we demonstrate that salt-dissociated "hsp90-free" AhR from several species still retains the ability to specifically bind ligand ([3H]TCDD). Tritium 164-166 aryl hydrocarbon receptor Rattus norvegicus 83-86 9562184-4 1998 This decrease probably occurred via inhibition of GAT-2 or GAT-3 activity since their inhibitor, beta-alanine, induced a decrease in [3H]-GABA uptake in punches of sham-operated rats (-28%), but not in punches of 5,7-DHT-treated rats, demonstrating that serotonin terminal degeneration had already impaired the beta-alanine-sensitive component of GABA uptake. Tritium 134-136 solute carrier family 6 member 11 Rattus norvegicus 59-64 9556603-7 1998 Thus, [3H]CGP 61594 is the first antagonist radioligand that reliably distinguishes the glycine site of NMDA receptor subtypes. Tritium 7-9 glutamate ionotropic receptor NMDA type subunit 1 L homeolog Xenopus laevis 104-117 9153238-2 1997 The [3H]thymidine incorporation in A7r5 overexpressed with PKC delta (DVs) was suppressed to 37.1 +/- 16.3% (mean +/- S.D.) Tritium 5-7 protein kinase C, gamma Rattus norvegicus 59-62 9153238-4 1997 The reduction of [3H]thymidine incorporation was strongly correlated with overexpressed PKC levels. Tritium 18-20 protein kinase C, gamma Rattus norvegicus 88-91 9574559-3 1998 LTB4 stimulated PLA2 over a similar concentration range that activated the NADPH oxidase, although kinetic studies revealed that the release of [3H]AA (t1/2 approximately 2 s) preceded H2O2 generation (t1/2 > 30 s). Tritium 145-147 prostaglandin reductase 1 Cavia porcellus 0-4 9573529-6 1998 IGF-I dose-dependently increased [3H]-thymidine incorporation. Tritium 34-36 insulin-like growth factor 1 Rattus norvegicus 0-5 9574559-6 1998 Additional studies performed with the protein kinase C inhibitor, Ro 31-8220, and under conditions in which Ca2+ mobilization was abolished, provided further evidence that LTB4 released [3H]AA independently of signal molecules derived from the hydrolysis of phosphatidylinositol(4,5)bisphosphate by PLC. Tritium 187-189 prostaglandin reductase 1 Cavia porcellus 172-176 9154329-4 1997 In DAT cells lowering the Na+ concentration to 0, 5 or 10 mM caused an increase in 3H-efflux. Tritium 83-85 solute carrier family 6 member 3 Homo sapiens 3-6 9154329-13 1997 In cross-loading experiments, 5 mM Na(+)- or 0 Cl(-)-induced efflux was much lower from [3H]-noradrenaline-loaded DAT, than NAT cells and was sensitive to mazindol, but not to desipramine. Tritium 89-91 solute carrier family 6 member 3 Homo sapiens 114-117 9572302-0 1998 In vitro binding properties in rat brain of [3H]Ro 25-6981, a potent and selective antagonist of NMDA receptors containing NR2B subunits. Tritium 45-47 glutamate ionotropic receptor NMDA type subunit 2B Rattus norvegicus 123-127 9593954-3 1998 Saturation binding analysis with [3H][d-Ala2,N-methyl-Phe4,Gly5-ol]enkephalin (DAMGO: a mu-opioid selective agonist) and [3H]naltrindole (a delta-opioid selective antagonist) revealed no age-related changes in Bmax for either the mu or delta-opioid receptors. Tritium 34-36 proenkephalin Rattus norvegicus 67-77 9572302-5 1998 Overall, the distribution of [3H]Ro 25-6981 binding sites correlated well with that of NR2B (but not NR2A) transcripts, revealed by in situ hybridization histochemistry. Tritium 30-32 glutamate ionotropic receptor NMDA type subunit 2B Rattus norvegicus 87-91 9572302-6 1998 The high affinity of [3H]Ro 25-6981 for NR2B-containing receptors renders this compound the ligand of choice to study the regulation of NR2B-containing receptor expression. Tritium 22-24 glutamate ionotropic receptor NMDA type subunit 2B Rattus norvegicus 40-44 9192741-7 1997 [3H]Sph was stable and not metabolized at all in plasma, but was rapidly incorporated into platelets and metabolized mainly to Sph-1-P in platelet-rich plasma. Tritium 1-3 ankyrin 1 Homo sapiens 127-132 9192741-8 1997 [3H]Sph-1-P was found to be unchanged in plasma, revealing that plasma does not contain the enzymes needed for Sph-1-P degradation. Tritium 1-3 ankyrin 1 Homo sapiens 4-9 9366495-3 1997 Importantly, when Caco-2 cells were incubated with tritium-labelled 25-hydroxyvitamin D3 (25(OH)D3) for up to 2 h they produced almost exclusively a metabolite, which was identified as 1alpha,25(OH)2D3 by co-chromatography with the synthetic standard in two different HPLC systems, and by a radioligand assay showing an identical binding affinity to the intestinal nuclear vitamin D receptor. Tritium 51-58 vitamin D receptor Homo sapiens 373-391 9151291-5 1997 [3H]Phorbol 12,13-dibutyrate ([3H]PDB) binding to neutrophil cytosolic PKC was significantly suppressed by cycloheterophyllin. Tritium 1-3 protein kinase C, beta Rattus norvegicus 71-74 9600674-8 1998 We suggest that the early decrease of GLT-1 protein might raise the extrasynaptic glutamic acid concentration, and contribute to the loss of motor neurons in affected mice, resulting in low [3H]glutamate uptake, low EAAC1 immunoreactivity and neurological deficits. Tritium 191-193 solute carrier family 1 (glial high affinity glutamate transporter), member 2 Mus musculus 38-43 9605945-5 1998 Cell proliferation with TGF-alpha, TGF-beta, KGF, or HGF was examined in terms of [3H]-thymidine uptake. Tritium 83-85 hepatocyte growth factor Rattus norvegicus 53-56 9566852-10 1998 With respect to [3H]thymidine incorporation into DNA, HOE 901 had similar effects as normal human insulin, while Asp(B10)insulin showed increased [3H]thymidine incorporation. Tritium 147-149 ectonucleotide pyrophosphatase/phosphodiesterase 3 Homo sapiens 113-120 9555086-7 1998 NMB caused a half-maximal increase in [Ca2+]i at 0.2 nM, in [3H]IP and PLD at 1 nM, and half-maximal receptor occupation at 1.2 nM. Tritium 61-63 neuromedin B Rattus norvegicus 0-3 9523849-8 1998 The results indicate that PCNA protein level as determined by Western blot analysis, but not PCNA mRNA expression, correlates with PCNA immunostaining and 3H-thymidine incorporation in the regenerating liver. Tritium 155-157 proliferating cell nuclear antigen Rattus norvegicus 26-30 13677667-5 1997 A decrease in the [3H]ketanserin specific binding in the striatum of cataleptic rats and CBA mice was found indicating a decrease in 5-HT2A receptor density. Tritium 19-21 5-hydroxytryptamine (serotonin) receptor 2A Mus musculus 133-148 9525955-10 1998 Like EE treatment, the selective uptake of [3H]CE-labeled HDL by the two hepatic cell types in vivo correlated with the changes in expression of SR-BI. Tritium 44-46 scavenger receptor class B, member 1 Rattus norvegicus 145-150 9544796-2 1998 The affinity of [3H]tamsulosin at tissue and cloned alpha1A- and alpha1B-adrenoceptors was somewhat greater and smaller, respectively, than that of [3H]prazosin. Tritium 17-19 calcium voltage-gated channel subunit alpha1 A Homo sapiens 52-59 9512456-9 1998 Accordingly, AEA as well as FAAH inhibitors, including arachidonoyltrifluoromethyl ketone (ATFMK), blocked [3H]2-AG hydrolysis by N18TG2 and RBL-2H3 membranes, whereas 2-AG inhibited [14C]AEA hydrolysis. Tritium 108-110 fatty acid amide hydrolase Mus musculus 28-32 9492296-5 1998 Bio-Gel P-6 column chromatography of a mixture of the tritium-labeled glycopeptide/oligosaccharides obtained by pronase-digestion followed by mild alkaline borohydride treatment showed that distribution of the sialylated core 2 oligosaccharides is different among CD45 isoforms. Tritium 54-61 protein tyrosine phosphatase receptor type C Homo sapiens 264-268 9111037-7 1997 Heparin and heparan sulfate (HS), but not dermatan sulfate, chondroitin sulfates A and C, hyaluronic acid, and K5 polysaccharide, competed with 3H-labeled heparin for binding to immobilized GST-Tat and inhibited HIV-LTR transactivation induced by extracellular GST-Tat. Tritium 144-146 Tat Human immunodeficiency virus 1 265-268 9109912-2 1997 To determine whether the DA reduction was accompanied by loss of DA nerve terminals, we measured levels of the DA transporter ([3H]WIN, 35,428 binding; DA transporter protein) and the vesicular monoamine transporter ([3H]DTBZ binding) in the putamen of these patients. Tritium 128-130 solute carrier family 6 member 3 Homo sapiens 111-125 9457079-5 1998 The majority of the cell-associated radioactivity found in keratinocytes incubated for 0.5 to 24 h with either free or RBP bound [3H]retinol was present as [3H]retinyl ester irrespective of the mode of delivery. Tritium 130-132 retinol binding protein 4 Homo sapiens 119-122 9494120-1 1998 Recombinant fragments of the kainate-selective glutamate recepto subunit GluR-6 were expressed in insect cells and analysed for [3H]kainate binding activity in order to characterize the structural determinants responsible for ligand recognition. Tritium 129-131 glutamate ionotropic receptor kainate type subunit 2 Homo sapiens 73-79 9225278-7 1997 The decrease in [3H]sumatriptan binding in the presence of 200 nM ketanserin, indicative of the presence of 5-HT1D receptors, was very limited throughout the human brain, only reaching 20% of total specific binding over the periaqueductal grey. Tritium 17-19 5-hydroxytryptamine receptor 1D Homo sapiens 108-114 9225282-6 1997 High affinity (Kd approximately 10 nM) [3H]5-HT binding was detected to membranes obtained from Cos-7 cells transiently expressing the guinea pig 5-HT1F receptor. Tritium 40-42 5-hydroxytryptamine receptor 1F Homo sapiens 146-152 9546654-6 1998 Pharmacological characterization of crude membrane preparations of the recombinant yeast cells showed saturable binding of the glycine antagonist [3H]MDL105,519 with Kd values of 56.9 +/- 6.19 nM (NR1a/NR2A), 26.72 +/- 2.13 nM (NR1a/NR2B), and 21.22 +/- 1.64 nM (NR1a/NR2C), and bound capacities of 17.94 +/- 1.24 pmol/mg membrane protein (NR1a/NR2A), 11.45 +/- 0.67 pmol/mg (NR1a/NR2B), and 16.15 +/- 0.86 (NR1a/NR2C) pmol/mg. Tritium 147-149 glutamate receptor, ionotropic, NMDA2B (epsilon 2) Mus musculus 233-237 9122262-4 1997 EEDQ decreased the specific binding of [3H]5HT to 5HT2c receptors. Tritium 39-42 5-hydroxytryptamine receptor 2C Homo sapiens 50-55 9543260-3 1998 Therefore, an increase in the NR1 and NR2B subunit proteins may be responsible for both the previously observed upregulation of [3H]dizocilpine binding in the cerebral cortex and the appearance of diazepam withdrawal signs. Tritium 129-131 glutamate ionotropic receptor NMDA type subunit 2B Rattus norvegicus 38-42 9546654-6 1998 Pharmacological characterization of crude membrane preparations of the recombinant yeast cells showed saturable binding of the glycine antagonist [3H]MDL105,519 with Kd values of 56.9 +/- 6.19 nM (NR1a/NR2A), 26.72 +/- 2.13 nM (NR1a/NR2B), and 21.22 +/- 1.64 nM (NR1a/NR2C), and bound capacities of 17.94 +/- 1.24 pmol/mg membrane protein (NR1a/NR2A), 11.45 +/- 0.67 pmol/mg (NR1a/NR2B), and 16.15 +/- 0.86 (NR1a/NR2C) pmol/mg. Tritium 147-149 glutamate receptor, ionotropic, NMDA2B (epsilon 2) Mus musculus 381-385 9534885-7 1998 Both IL-1 alpha and IL-1 beta showed a stronger growth-stimulatory activity on DF-derived fibroblasts in a dose-dependent manner than normal fibroblasts, and the percent 3H-TdR uptake of DF was 1.4-fold (IL-1 alpha; 1,000 U/ml) and 1.3-fold (IL-1 beta; 1,000 U/ml) as compared with normal fibroblasts; however, the differences did not reach any significance. Tritium 170-172 interleukin 1 alpha Homo sapiens 5-15 9417111-1 1998 The bacteriophage T4 PinA protein inhibited degradation of [3H]alpha-methyl casein by purified Lon protease from Escherichia coli, but inhibition was noncompetitive with respect to casein. Tritium 60-62 putative ATP-dependent Lon protease Escherichia coli 95-98 9098555-0 1997 Stress persistently increases NMDA receptor-mediated binding of [3H]PDBu (a marker for protein kinase C) in the amygdala, and re-exposure to the stressful context reactivates the increase. Tritium 65-67 protein kinase C, gamma Rattus norvegicus 87-103 9673794-3 1998 In radioligand binding experiments using brain membranes from rat, rabbit, and guinea-pig, [3H]nociceptin bound a single population of binding sites with high affinity (KD values ranging from 0.049 to 0.124 nM) and capacity (Bmax ranging from 143 to 254 fmol.mg-1 protein). Tritium 92-94 prepronociceptin Rattus norvegicus 95-105 9050900-4 1997 In accord with the electrophysiological results, fluoxetine inhibited the binding of [3H]5HT to 5HT2C receptors expressed in HeLa cells (Ki approximately 65-97 nM), and the binding to 5HT receptors in rat cortex membranes was also inhibited but less efficiently (Ki approximately 56 microM). Tritium 86-88 5-hydroxytryptamine receptor 2C Homo sapiens 96-101 9815705-6 1997 Brief periods of exposure ( approximately 30 min) to SPA at the IC50 for growth increased incorporation of [3H]mannose. Tritium 108-111 surfactant protein A2 Homo sapiens 53-56 9458783-5 1998 In the full-thickness corpus mucosa, tetragastrin enhanced [3H]glucosamine incorporation into mucin but had no effect on [14C]threonine incorporation. Tritium 60-62 solute carrier family 13 member 2 Rattus norvegicus 94-99 9544732-6 1998 PTHrP-(1-34) also inhibited 5% FBS-induced increase in [3H]thymidine incorporation (37% of control at 1 micromol/l) and cell number of VSMCs (33% of control at 1 micromol/l) in a concentration-dependent manner. Tritium 56-58 parathyroid hormone-like hormone Rattus norvegicus 0-5 9484857-4 1998 MEN 11420 competitively bound with high affinity to the human NK2 receptor stably transfected in CHO cells, displacing radiolabelled [125I]-neurokinin A and [3H]-SR 48968 with Ki values of 2.5+/-0.7 nM (n = 6) and 2.6+/-0.4 nM (n = 3), respectively. Tritium 158-160 tachykinin receptor 2 Homo sapiens 62-74 9566307-8 1998 Intensive labelling of cells with 3H-Tdr was used to identify long-lived mammary epithelial cells which retained 3H-Tdr 2 weeks following labelling (i.e., following 3 estrous cycles). Tritium 113-115 tudor domain containing 6 Mus musculus 116-121 9397158-3 1998 The mechanism involves tethering of the factor to SMC, since [3H]-elastin pre-incubated with serum or endothelial cell (EC)-conditioned medium or SMC pre-treated with serum accelerates binding of elastin and tyrosine-kinase related elastase activity. Tritium 62-64 elastin Homo sapiens 66-73 9397158-3 1998 The mechanism involves tethering of the factor to SMC, since [3H]-elastin pre-incubated with serum or endothelial cell (EC)-conditioned medium or SMC pre-treated with serum accelerates binding of elastin and tyrosine-kinase related elastase activity. Tritium 62-64 elastin Homo sapiens 196-203 9081125-6 1997 Lysyl oxidase activity in the media was quantitated with a tritium-release bioassay against an insoluble 3H-labeled aortic clastin substrate. Tritium 59-66 lysyl oxidase Rattus norvegicus 0-13 9081125-6 1997 Lysyl oxidase activity in the media was quantitated with a tritium-release bioassay against an insoluble 3H-labeled aortic clastin substrate. Tritium 105-107 lysyl oxidase Rattus norvegicus 0-13 9101540-3 1997 [3H]Forskolin binding significantly decreased in the striatum, hippocampal CA3 sector, dentate gyrus, hilus, thalamus, substantia nigra and cerebellum of 24-month-old (aged) rats, as compared with 6-month-old (adult) animals. Tritium 1-3 carbonic anhydrase 3 Rattus norvegicus 75-78 9667763-1 1998 Incubation of tritium labeled dopamine (3HDA) with a preparation of rat heart or bovine serum albumin in vitro, or the intravenous injection of 3HDA to rats, gives acid insoluble, tritium labeled material that is slowly converted to soluble labeled material (SLM) by heating in dilute perchloric acid. Tritium 14-21 albumin Rattus norvegicus 94-101 9500571-2 1998 CEase increased the uptake of [3H]cholesterol from bile salt mixed-micelles by 2.5- to 3.0-fold and its esterification by greater than 25-fold. Tritium 31-33 carboxyl ester lipase Homo sapiens 0-5 9493867-5 1998 [3H][D-Ala2,D-Leu5]enkephalin labeled a single binding site in membranes prepared from MDOR cells under several different assay conditions including BIT-pretreatment. Tritium 1-4 opioid receptor, delta 1 Mus musculus 87-91 9493867-7 1998 [3H][D-Ala2,D-Leu5]enkephalin labeled two binding sites in mouse brain membranes depleted of mu receptors by pretreatment with BIT: the delta(ncx1) site (high affinity for DPDPE and deltorphin) and the delta(ncx2) site (low affinity for DPDPE and deltorphin). Tritium 1-3 solute carrier family 8 (sodium/calcium exchanger), member 1 Mus musculus 136-146 9038174-1 1997 Sphingosine, sphinganine, and other long-chain (sphingoid) bases are highly bioactive intermediates of sphingolipid metabolism that have diverse effects when added to cells, including the inhibition of protein kinase C (PKC) as evaluated by both enzymatic activity and [3H]phorbol dibutyrate ([3H]PDBu) binding. Tritium 270-272 protein kinase C, delta Mus musculus 220-223 9065728-7 1997 MAFP displaced [3H]CP-55940 binding to the CB1 cannabinoid receptor with an IC50 of 20 nM vs 40 nM for anandamide. Tritium 16-18 cannabinoid receptor 1 Rattus norvegicus 43-46 9493867-7 1998 [3H][D-Ala2,D-Leu5]enkephalin labeled two binding sites in mouse brain membranes depleted of mu receptors by pretreatment with BIT: the delta(ncx1) site (high affinity for DPDPE and deltorphin) and the delta(ncx2) site (low affinity for DPDPE and deltorphin). Tritium 1-3 solute carrier family 8 (sodium/calcium exchanger), member 2 Mus musculus 202-212 9570636-9 1998 However, the stratum oriens of the hippocampal CA1 sector showed a significant reduction in [3H]FK506 binding 48 h and 7 days after ischemia. Tritium 93-95 carbonic anhydrase 1 Rattus norvegicus 47-50 9466707-0 1997 Dopamine transporter development in postnatal rat striatum: an autoradiographic study with [3H]WIN 35,428. Tritium 92-94 solute carrier family 6 member 3 Homo sapiens 0-20 9466707-3 1997 [3H]WIN 35,428 is a cocaine analog that is useful for studying the distribution and density of the dopamine transporter in striatum and other brain regions. Tritium 1-3 solute carrier family 6 member 3 Homo sapiens 99-119 9466707-4 1997 The postnatal development of the dopamine transporter in the rat striatum was measured by quantitative autoradiography with [3H]WIN 35,428. Tritium 125-127 solute carrier family 6 member 3 Homo sapiens 33-53 9020879-10 1997 VLDL turnover studies with autologous injections of [3H]TG-VLDL in vivo showed that the VLDL fractional catabolic rate (FCR) was decreased by up to 50% in the apoC1-deficient mice when compared with control mice (10.5 +/- 3.4 versus 21.0 +/- 1.2/h of pool TG). Tritium 53-55 apolipoprotein C-I Mus musculus 159-164 9013448-4 1997 Expression of cyclin D1, p16INK4 and p21Cip1 was observed from 24 h after the infection, and the expression increased up to 48 h. AxCAp16 and AxCAp21 caused a significant inhibition in the [3H]-thymidine incorporation to 47% and 76%, respectively. Tritium 190-192 cyclin-dependent kinase inhibitor 2A Rattus norvegicus 25-32 9500202-8 1998 In contrast to downregulation of RFC, the much greater uptake of [3H]MTX was observed in the MOLT-3/trimetrexate (TMQ)800-MTX10000 in parallel with increased RFC1 expression. Tritium 66-68 metaxin 1 Homo sapiens 69-72 8978752-2 1997 By using [3H]SR 141716A, the specific CB1 receptor antagonist, we demonstrate in cortical neurons the presence of a high density of specific binding sites (Bmax = 139 +/- 9 fmol/mg of protein) displaying a high affinity (KD = 0.76 +/- 0.09 nM). Tritium 10-12 cannabinoid receptor 1 Rattus norvegicus 38-41 9435526-4 1997 At concentrations of 10(-12) M and greater, adrenomedullin produced a dose-dependent increase in cell number and [3H]thymidine incorporation in cultures of fetal rat osteoblasts. Tritium 114-116 adrenomedullin Rattus norvegicus 44-58 9435526-7 1997 Adrenomedullin also increased [3H]thymidine incorporation into cultured neonatal mouse calvaria but, unlike amylin, did not reduce bone resorption in this model. Tritium 31-33 adrenomedullin Mus musculus 0-14 9516938-5 1998 The synergistic effect was not due to augmented inhibition of thymidylate synthase, as determined by the measurement of thymidylate synthase activity by tritium release from [5-3H]2"-deoxyuridine. Tritium 153-160 thymidylate synthetase Homo sapiens 120-140 9427656-7 1997 [3H]GW 2433 is an effective radioligand for use in PPARdelta competition-binding assays. Tritium 1-3 peroxisome proliferator activated receptor delta Homo sapiens 51-60 9415401-8 1997 However, in the reverse reaction, [3H]-3alpha-androstanediol was oxidized first to [3H]-androsterone and then to [3H]-androstanedione, revealing that the expressed protein possessed both 3alpha- and 17beta-HSD activities. Tritium 35-37 aldo-keto reductase family 1 member C4 Homo sapiens 187-209 9415401-8 1997 However, in the reverse reaction, [3H]-3alpha-androstanediol was oxidized first to [3H]-androsterone and then to [3H]-androstanedione, revealing that the expressed protein possessed both 3alpha- and 17beta-HSD activities. Tritium 35-38 aldo-keto reductase family 1 member C4 Homo sapiens 187-209 8996218-2 1997 SIB-1765F is a subtype selective NAChR agonist that displaces [3H]-nicotine binding with an IC50 of 4.6 nM and [3H]-cytisine binding with an IC50 of 12.2 nM which is 2000- to 6000-fold lower than its displacement of [3H]-QNB or [125I]-alpha-bungarotoxin. Tritium 63-65 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 33-38 9070190-8 1997 Likewise, the same amount of the 0.5 M peak accounted for the majority of degradation (30.8%) of 3H-labeled HSPG. Tritium 97-99 CD44 molecule (Indian blood group) Homo sapiens 108-112 9116589-5 1997 The density of brain MAO-B sites labeled by [3H]Ro 19-6327 (lazabemide) in suicides was no different to that in age-matched controls. Tritium 45-47 monoamine oxidase B Homo sapiens 21-26 8944652-6 1996 Dual 22Na+ and [3H]mannitol flux studies indicated that the decrease in resistance in tMK cells was attributable to increased paracellular flow. Tritium 16-18 deoxythymidylate kinase Mus musculus 86-89 8908192-3 1996 DT cells incubated in the presence of 1 microM dexamethasone (DEX) for 24 hr expressed a relatively high level of membrane-bound Ha-Ras protein, BK B2 receptor mRNA, and B2 receptor binding as determined by Western blotting with anti-Ha-Ras antibodies, reverse transcriptase-polymerase chain reaction using B2 receptor-specific primers, and specific [3H]BK binding, respectively. Tritium 351-353 Harvey rat sarcoma virus oncogene Mus musculus 129-135 9457843-3 1998 Donor cells rapidly incorporated [3H]cPD1. Tritium 34-36 cerebellar ataxia, infantile nonprogressive, autosomal recessive Homo sapiens 37-41 8884770-3 1996 In the hippocampus of aged rats, [3H] alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate binding in CA1 and [3H]kainate binding in CA3 were reduced. Tritium 111-113 carbonic anhydrase 3 Rattus norvegicus 133-136 9457843-4 1998 The cell extract but not the membrane fraction of the donor strain exhibited significant [3H]cPD1-binding activity. Tritium 90-92 cerebellar ataxia, infantile nonprogressive, autosomal recessive Homo sapiens 93-97 9457843-6 1998 The cell extract of a strain carrying the traA-bearing multicopy plasmid (pDLHH21) also exhibited high [3H]cPD1-binding activity. Tritium 104-106 cerebellar ataxia, infantile nonprogressive, autosomal recessive Homo sapiens 107-111 8892525-4 1996 The Kd values of [3H]mesulergine binding to 5HT2C receptors were not different in these brain regions of P rats compared with NP rats. Tritium 17-20 5-hydroxytryptamine receptor 2C Rattus norvegicus 44-49 8891693-3 1996 Segments cultured in nM (0.1-1.7 nM) concentrations of insulin, truncated IGF-I (tIGF-I), long R3IGF-I, or IGF-II exhibited an increase in [3H]thymidine incorporation compared with control segments. Tritium 140-142 insulin-like growth factor 2 Rattus norvegicus 107-113 9457843-7 1998 A recombinant TraA exhibited a dissociation constant of 0.49 +/- 0.08 nM against [3H]cPD1. Tritium 82-84 cerebellar ataxia, infantile nonprogressive, autosomal recessive Homo sapiens 85-89 9457843-8 1998 iPD1 competitively inhibited [3H]cPD1 binding to TraA, whereas pheromones and inhibitors relating to other plasmid systems did not. Tritium 30-32 cerebellar ataxia, infantile nonprogressive, autosomal recessive Homo sapiens 33-37 8864900-3 1996 Stimulation of [3H]thymidine incorporation (TDR) into DNA by bFGF was determined in the presence of phorbol myristate acetate of (PMA) to down-regulate the protein kinase C (PKC) pathway, genistein, an inhibitor of tyrosine kinase and H-7, a PKC inhibitor, bFGF 10(-8) M and PMA 10(-7) M increased TDR by 242 and 245%, respectively. Tritium 16-18 solute carrier family 9 member A2 Rattus norvegicus 235-238 9457843-10 1998 A strain carrying the traC-bearing multicopy plasmid (pDLES23) exhibited significant [3H]cPD1-binding activity. Tritium 86-88 cerebellar ataxia, infantile nonprogressive, autosomal recessive Homo sapiens 89-93 9605415-4 1998 EM-652 is 20x more potent than ICI 164384 and Droloxifene while it is 400 times more potent than Toremifene in displacing [3H]E2 from the rat uterine estrogen receptor. Tritium 123-125 estrogen receptor 1 Rattus norvegicus 150-167 8819505-1 1996 3H-antagonists are known to bind to the alpha-2A adrenergic receptor with higher affinity in glycylglycine buffer than in Tris buffer. Tritium 0-2 adrenoceptor alpha 2A Homo sapiens 40-68 9779197-9 1998 When hypoxia was combined with CHI, significant increase in [3H] GLU binding to NMDA receptors and significant decrease in [3H]-muscimol binding to GABAA receptors were observed in CA1 and CA3 at 1 h and 24 h post-insult. Tritium 124-126 carbonic anhydrase 1 Rattus norvegicus 181-184 8797470-2 1996 These markers included DA itself, three different estimates of the density of the DA transporter (DAT) ([3H])GBR 12,935 and [3H]WIN 35,428 binding; DAT protein immunoreactivity), and one estimate of the vesicular monoamine transporter (VMAT2; [3H]DTBZ binding). Tritium 105-107 solute carrier family 6 member 3 Homo sapiens 82-96 8797470-2 1996 These markers included DA itself, three different estimates of the density of the DA transporter (DAT) ([3H])GBR 12,935 and [3H]WIN 35,428 binding; DAT protein immunoreactivity), and one estimate of the vesicular monoamine transporter (VMAT2; [3H]DTBZ binding). Tritium 105-107 solute carrier family 6 member 3 Homo sapiens 98-101 9666320-3 1998 Furthermore, addition of an anti-EGF receptor antibody not only reduced the effect of exogenous EGF (100 ng/ml) on DNA synthesis but decreased basal 3H-thymidine incorporation. Tritium 149-151 epidermal growth factor Homo sapiens 33-36 8702845-2 1996 In order to delineate structural motifs regulating substrate affinity and recognition for the human dopamine transporter (DAT), we assessed [3H]dopamine uptake kinetics and [3H]CFT binding characteristics of COS-7 cells transiently expressing mutant DATs in which the COOH terminus was truncated or substituted. Tritium 141-143 solute carrier family 6 member 3 Homo sapiens 100-120 8702845-2 1996 In order to delineate structural motifs regulating substrate affinity and recognition for the human dopamine transporter (DAT), we assessed [3H]dopamine uptake kinetics and [3H]CFT binding characteristics of COS-7 cells transiently expressing mutant DATs in which the COOH terminus was truncated or substituted. Tritium 141-143 solute carrier family 6 member 3 Homo sapiens 122-125 8702845-2 1996 In order to delineate structural motifs regulating substrate affinity and recognition for the human dopamine transporter (DAT), we assessed [3H]dopamine uptake kinetics and [3H]CFT binding characteristics of COS-7 cells transiently expressing mutant DATs in which the COOH terminus was truncated or substituted. Tritium 174-176 solute carrier family 6 member 3 Homo sapiens 100-120 9453158-3 1998 Saccharomyces cerevisiae transformant S-12, harbouring the CDR1 gene, accumulated about 3-fold less [3H] beta-estradiol and about 2-fold less [3H]corticosterone than the non-transformed strain. Tritium 101-103 cerebellar degeneration related protein 1 Homo sapiens 59-63 8702845-2 1996 In order to delineate structural motifs regulating substrate affinity and recognition for the human dopamine transporter (DAT), we assessed [3H]dopamine uptake kinetics and [3H]CFT binding characteristics of COS-7 cells transiently expressing mutant DATs in which the COOH terminus was truncated or substituted. Tritium 174-176 solute carrier family 6 member 3 Homo sapiens 122-125 8702845-3 1996 Complete truncation of the carboxyl tail from Ser582 allowed for the expression of biphasic [3H]dopamine uptake kinetics displaying both a low capacity (Vmax approximately 0.4 pmol/10(5) cells/min) high affinity (Km approximately 300 nM) component and one exhibiting low affinity (Km approximately 15 microM] and high capacity (Vmax approximately 5 pmol/10(5)cells/min) with a concomitant 40% decrease in overall apparent Vmax relative to wild type (WT) DAT. Tritium 93-95 solute carrier family 6 member 3 Homo sapiens 454-457 8702845-7 1996 In marked contrast, DAT truncation/substitution mutants displayed significantly reduced high affinity [3H]CFT binding interactions with estimated Ki values for dopamine and numerous other substrates and inhibitors tested from 10-100-fold lower than that observed for WT DAT. Tritium 103-105 solute carrier family 6 member 3 Homo sapiens 20-23 8702845-7 1996 In marked contrast, DAT truncation/substitution mutants displayed significantly reduced high affinity [3H]CFT binding interactions with estimated Ki values for dopamine and numerous other substrates and inhibitors tested from 10-100-fold lower than that observed for WT DAT. Tritium 103-105 solute carrier family 6 member 3 Homo sapiens 270-273 8706017-11 1996 Addition of 30 ng/ml BDNF decreased [3H]vinblastine accumulation to 58% of control in SY5Y cells and decreased [3H]vinblastine accumulation to 62% of control in TrkB-expressing SY5Y cells. Tritium 37-39 brain derived neurotrophic factor Homo sapiens 21-25 8706017-11 1996 Addition of 30 ng/ml BDNF decreased [3H]vinblastine accumulation to 58% of control in SY5Y cells and decreased [3H]vinblastine accumulation to 62% of control in TrkB-expressing SY5Y cells. Tritium 112-114 brain derived neurotrophic factor Homo sapiens 21-25 8756459-4 1996 When the nuclear extract containing a 10-fold molar excess of CRABP I was incubated with RAR alpha extract in the presence of [3H]RA and resolved on an FPLC size-exclusion column, a 20% decrease in the bound radioactivity in the RAR alpha fraction was accompanied by a proportional increase in the CRABP I fraction. Tritium 127-129 cellular retinoic acid binding protein I Mus musculus 62-69 9453158-3 1998 Saccharomyces cerevisiae transformant S-12, harbouring the CDR1 gene, accumulated about 3-fold less [3H] beta-estradiol and about 2-fold less [3H]corticosterone than the non-transformed strain. Tritium 143-145 cerebellar degeneration related protein 1 Homo sapiens 59-63 9435202-2 1998 Ang II and Ang-(1-7) enhanced AA release in cells prelabeled with [3H]AA. Tritium 67-69 angiogenin Oryctolagus cuniculus 0-3 8869589-9 1996 IGFBP-4 proteolyzed in the conditioned medium from cells treated with PTH and 17 beta-estradiol was less effective at inhibiting IGF-I-stimulated [3H]thymidine incorporation into DNA compared with that proteolyzed in the conditioned medium from PTH-treated cells. Tritium 147-149 insulin like growth factor binding protein 4 Homo sapiens 0-7 8768691-6 1996 The increase in CRF-BP expression was not due to increased cell proliferation as measured by [3H]thymidine incorporation. Tritium 94-96 corticotropin releasing hormone binding protein Rattus norvegicus 16-22 8700130-3 1996 The accumulation of [3H]VP-16 was 2-3-fold lower in the resistant cells (HL-60/DOX 0.05) than in similarly treated parent-sensitive cells (HL-60/S). Tritium 21-23 host cell factor C1 Homo sapiens 24-29 8856661-7 1996 These metabolites specifically displaced [3H]-9cRA from RXR with Ki values of 4 microM, indicating that their transcriptional effects are mediated by direct receptor interactions. Tritium 42-44 retinoid X receptor alpha Homo sapiens 56-59 8878051-2 1996 [3H]LSD saturation studies indicated that 5-HT1D receptor expression levels were slightly higher in the 1D beta cell line (Bmax = 1334 +/- 134 fmol/mg protein) than in the 1D alpha cell line (Bmax = 900 +/- 218 fmol/mg protein). Tritium 1-3 5-hydroxytryptamine receptor 1D Homo sapiens 42-48 8675579-7 1996 The expressed mutant VDR displayed a normal binding affinity (Kd = 1.2 x 10(-10) mol/L) for [3H]1,25-(OH)2D3 as determined by Scatchard analysis. Tritium 93-95 vitamin D receptor Homo sapiens 21-24 8882288-7 1996 Both IGF-I (100 ng ml-1) and IGF-II (100 ng ml-1) and their respective analogues, as well as insulin, all at the same concentration, significantly increased DNA synthesis as determined by [3H]thymidine incorporation (n = 5). Tritium 189-191 IGFI Bos taurus 5-10 8882288-7 1996 Both IGF-I (100 ng ml-1) and IGF-II (100 ng ml-1) and their respective analogues, as well as insulin, all at the same concentration, significantly increased DNA synthesis as determined by [3H]thymidine incorporation (n = 5). Tritium 189-191 insulin like growth factor 2 Bos taurus 29-35 8698628-7 1996 IN157/VP-1 cells showed decreased accumulation of [3H]VP16 and overexpression of MRP gene, but not of MDR1. Tritium 51-53 host cell factor C1 Homo sapiens 54-58 8783231-2 1996 The level of [3H]vesamicol binding to a terminal vesicular acetylcholine transporter is inversely related to the decline in cortical choline acetyltransferase activity in Alzheimer"s disease, which may be due to compensatory processes within surviving cholinergic terminals. Tritium 14-16 choline O-acetyltransferase Rattus norvegicus 133-158 8783231-4 1996 In Rett syndrome, a significant decline in choline acetyltransferase activity within the putamen and thalamus was directly correlated with a decline in [3H]vesamicol binding. Tritium 153-155 choline O-acetyltransferase Rattus norvegicus 43-68 9812726-6 1996 When the concentration-dependent inhibition of [3H] Oxy binding by SK & F was compared with Lyp and L-366948 [(cyclo-(L-Pro-D-2-naphthyl-Ala-L-lle-D-pipecolic acid-L-pipecolic acid-D-His)], a potent antagonist specific for Oxy receptors, the Ki values for SK & F, Lyp, and L-366948 were 1.73 +/- 0.32, 14.36 +/- 1.73, and 1.79 +/- 0.35 nmol.L-1, respectively. Tritium 48-50 oxytocin/neurophysin I prepropeptide Homo sapiens 52-55 8660352-7 1996 Both CNP and 8-bromo cGMP dose-dependently decreased [3H]thymidine uptake, without affecting alkaline phosphatase activity. Tritium 54-56 natriuretic peptide C Homo sapiens 5-8 8832589-7 1996 Exposition of rats to the decapitation procedure increased [3H]-CCK-8 binding in the frontal and cerebral (whole-frontal) cortex. Tritium 60-62 cholecystokinin Rattus norvegicus 64-67 8769688-7 1996 In contrast, with low serum concentration (0.1% FBS) the addition of IGF-I and -II to bovine cSMC cultures resulted in a slight increase in cell number, protein content and [3H]thymidine incorporation as described in previous studies. Tritium 174-176 IGFI Bos taurus 69-82 8843491-17 1996 Adenosine deaminase (0.5 U/ml) abolished both the 1S,3R-ACPD (100 microM)-induced [3H]-cyclic AMP accumulation and the synergistic interaction of this compound with NA (100 microM). Tritium 83-85 adenosine deaminase Rattus norvegicus 0-19 8726364-4 1996 During the initial growth period (days 1-10), addition of acidic FGF (100 micrograms/ml) to actively proliferating cells increased (P < 0.05) 3H-thymidine uptake (2,515 +/- 137, mean +/- SEM vs. 5,884 +/- 818 cpm/10(4) cells). Tritium 145-147 fibroblast growth factor 1 Rattus norvegicus 65-68 8636125-6 1996 In addition, overexpression of Rab3A modestly inhibited Ca2+-evoked NE release, whereas Rab3B and a GTP binding mutant (Rab3B N135I) markedly stimulated the efficiency of [3H]NE secretion by PC12 cells (i.e. secretion normalized to total cell radioactivity). Tritium 172-174 RAB3B, member RAS oncogene family Rattus norvegicus 88-93 8636125-6 1996 In addition, overexpression of Rab3A modestly inhibited Ca2+-evoked NE release, whereas Rab3B and a GTP binding mutant (Rab3B N135I) markedly stimulated the efficiency of [3H]NE secretion by PC12 cells (i.e. secretion normalized to total cell radioactivity). Tritium 172-174 RAB3B, member RAS oncogene family Rattus norvegicus 120-125 8636125-7 1996 Expression of Rab3B and Rab3B N135I increased not only the efficiency of NE secretion but also the accumulation of [3H]NE into LDCVs (i.e. the secretory cargo available for secretion). Tritium 116-118 RAB3B, member RAS oncogene family Rattus norvegicus 14-19 8636125-7 1996 Expression of Rab3B and Rab3B N135I increased not only the efficiency of NE secretion but also the accumulation of [3H]NE into LDCVs (i.e. the secretory cargo available for secretion). Tritium 116-118 RAB3B, member RAS oncogene family Rattus norvegicus 24-29 9206115-3 1996 This study demonstrated that ET-1 (0.1 nmol/L to 100 nmol/L) increased the [3H] thymidine uptake in a dose-dependent manner in cultured bovine pulmonary artery smooth muscle cells (PASMC), which was enhanced by exposing PASMC to hypoxia (2% O2, 93% N2, 5% CO2). Tritium 76-78 endothelin 1 Bos taurus 29-33 8681046-3 1996 These changes were confirmed by receptor-ligand binding assays with [3H]-dexamethasone which showed a sixfold increase in average obese nuclear GR binding and a twofold reduction in cytosolic GR binding. Tritium 69-72 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 144-146 8851473-2 1996 Whereas nicotinic acetylcholine receptor affinity was determined using [3H]nicotine as the radioactive ligand, [3H]oxotremorine-M ([3H]Oxo-M) and [3H]quinuclidinyl benzilate ([3H]QNB), in some cases supplemented with [3H]pirenzepine ([3H]PZ), were used as radioligands for muscarinic acetyicholine receptors on rat brain membranes. Tritium 72-74 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 8-40 8819644-4 1996 In the transfected LTK- cell membranes with pRc/CMV-D1A receptor cDNA, a selective D1 dopamine antagonist, [3H]-SCH 23390, exhibited a Kd of 0.9 +/- 0.1 nmol/L and a Bmax of 0.35 +/- 0.05 pmol/mg protein (n = 5). Tritium 108-110 leukocyte tyrosine kinase Mus musculus 19-22 8818201-6 1996 IGF-I and IGF-II (but to a lesser extent) stimulation was due to an increased DNA synthesis (3H-thymidine uptake) as well as protein anabolism (incorporated proline). Tritium 93-95 insulin-like growth factor 2 Rattus norvegicus 10-16 8558445-12 1996 These results demonstrate that stable expression of the human alpha 4 beta 2 nAChR subunit combination can give rise to functional ion channels that bind [3H](-)-cytisine with high affinity, exhibit homologous regulation and evoke agonist-induced cation flux with pharmacological properties consistent with native neuronal alpha 4 beta 2 nAChR. Tritium 155-157 immunoglobulin binding protein 1 Homo sapiens 62-69 8950299-2 1996 The binding of [3H]forskolin to adenylate cyclase (AC), [3H]phorbol 12,13-dibutyrate (PDBu) to protein kinase C (PKC), [3H]SCH23390 binding to the dopamine D1 (DA-D1) receptor and [3H]spiperone binding to the dopamine D2 (DA-D2) receptor were measured using quantitative autoradiography. Tritium 16-18 protein kinase C, gamma Rattus norvegicus 95-111 8843957-5 1996 Incubation of uninduced or induced MEL cells with iron chelator pyridoxal isonicotinoyl hydrazone (PIH) or desferrioxamine (Desferal) and 3H-uridine decreased the level of the 3H-labeled (ALAS-E) mRNA. Tritium 138-140 aminolevulinic acid synthase 2, erythroid Mus musculus 188-194 8843957-6 1996 The treatment with either diferric transferrin or Fe-PIH increased the level of the 3H-labeled (ALAS-E) mRNA. Tritium 84-86 transferrin Mus musculus 35-46 8843957-6 1996 The treatment with either diferric transferrin or Fe-PIH increased the level of the 3H-labeled (ALAS-E) mRNA. Tritium 84-86 aminolevulinic acid synthase 2, erythroid Mus musculus 96-102 8788910-0 1995 Downregulation of muscarinic- and 5-HT1B-mediated modulation of [3H]acetylcholine release in hippocampal slices of rats with fimbria-fornix lesions and intrahippocampal grafts of septal origin. Tritium 65-67 5-hydroxytryptamine receptor 1B Rattus norvegicus 34-40 7489243-4 1995 In nondenuded organ cultures, elastolytic activity assessed by using [3H]elastin increased sixfold at day 3 after initiation of the culture (P < .01), a time earlier than the previously published increase in intimal smooth muscle cells (ISMCs). Tritium 70-72 elastin Homo sapiens 73-80 7498647-5 1995 RESULTS: Human colonic epithelial cells and Caco-2 cells synthesize and secrete PAF-AH as shown by in vitro hydrolysis of [3H]PAF to [3H]-lysoPAF in cell lysates and conditioned media. Tritium 123-125 phospholipase A2 group VII Homo sapiens 80-86 7595583-5 1995 Ischemia significantly decreased [3H]SR-95531 binding in hippocampal areas CA1 and CA3 and in the dentate gyrus 30 min after ischemia. Tritium 34-36 carbonic anhydrase 1 Homo sapiens 75-78 7498491-0 1995 The recombinant GABA transporter GAT1 is downregulated upon activation of protein kinase C. Treatment of human embryonic kidney 293 cells expressing the rat gamma-aminobutyric acid (GABA) transporter 1 (GAT1) with the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) was found to decrease the velocity of specific [3H]GABA uptake. Tritium 337-339 solute carrier family 6 member 12 Rattus norvegicus 157-201 7498491-0 1995 The recombinant GABA transporter GAT1 is downregulated upon activation of protein kinase C. Treatment of human embryonic kidney 293 cells expressing the rat gamma-aminobutyric acid (GABA) transporter 1 (GAT1) with the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) was found to decrease the velocity of specific [3H]GABA uptake. Tritium 337-339 solute carrier family 6 member 12 Rattus norvegicus 203-207 8550082-8 1995 In contrast, murine interleukin-3 (IL-3) in combination with MCP-1 (200 and 100 nM) provoked a greater release of histamine and [3H]5HT than the compounds alone. Tritium 129-131 chemokine (C-C motif) ligand 2 Mus musculus 61-66 7473192-1 1995 There is a consensus that high-affinity [3H]-L-nicotine binding sites in the mammalian brain, which are thought to represent a predominant form of central nervous system nicotinic acetylcholine receptor (nAChR) composed of alpha 4 and beta 2 subunits, are increased in number after chronic nicotine exposure. Tritium 41-44 immunoglobulin binding protein 1 Homo sapiens 223-241 8587658-3 1995 Both CCK-8S and CCKB receptor agonists BC 264 and Suc-Trp-N(Me)Nle-Asp-Phe-NH2 stimulate [3H]thymidine incorporation. Tritium 90-92 cholecystokinin Rattus norvegicus 5-8 8602897-8 1995 The prototypical 5-HT1 agonist 5-carboxy-amiditryptamine (5-CT) and the 5-HT1B/1D agonist sumatriptan (1-1,000 nM) induced a concentration-dependent inhibition of the electrically evoked release of [3H]5-HT from preloaded raphe slices. Tritium 199-201 5-hydroxytryptamine receptor 1B Rattus norvegicus 72-78 8602897-10 1995 The selective 5-HT1B agonist CP 93129 (10-10,000 nM), had no effect in raphe slices, but it dose dependently inhibited [3H]5-HT release from hippocampal slices where autoreceptors are of the 5-HT1B subtype. Tritium 120-122 5-hydroxytryptamine receptor 1B Rattus norvegicus 14-20 9374486-5 1997 ROAT1-mediated transport of 50 mu M [3H]PAH was independent of imposed changes in membrane potential. Tritium 37-39 solute carrier family 22 member 6 Rattus norvegicus 0-5 9394121-2 1997 The density of MAO-B sites was quantified by the specific binding of the selective inhibitor [3H]Ro 19-6327 (lazabemide) (8 nM) to cortical membranes. Tritium 94-96 monoamine oxidase B Homo sapiens 15-20 9351438-3 1997 LIF increased protein content and [3H]phenylalanine uptake in cardiomyocytes in a dose-dependent manner. Tritium 35-37 LIF, interleukin 6 family cytokine Rattus norvegicus 0-3 9378967-4 1997 [3H]TdR incorporation determined 2 days after CD9 costimulation was as potent as that induced by CD28 costimulation. Tritium 1-3 CD9 molecule Homo sapiens 46-49 9345273-4 1997 After incubation with [3H]RA, the level of retinoylated protein in NIH 3T3 cells was about 1.5-fold greater than in NIH Ha-ras-3T3 cells. Tritium 23-25 Harvey rat sarcoma virus oncogene Mus musculus 120-126 9325175-5 1997 Engineered Sfn insect cells infected with a recombinant baculovirus bearing the human dopamine transporter gene under the control of the polyhedrin promoter showed a > or = 5 times enhanced uptake of [3H]dopamine in comparison to similarly infected Sf9 cells. Tritium 204-206 solute carrier family 6 member 3 Homo sapiens 86-106 9369369-0 1997 Effects of NMDA receptor antagonists on delta1- and delta2-opioid receptor agonists-induced changes in the mouse brain [3H]DPDPE binding. Tritium 120-122 opioid receptor, delta 1 Mus musculus 40-74 9369369-10 1997 The results suggest that multiple injections of delta1- and delta2-opioid receptor agonists down-regulate delta1-opioid receptors of the brain by modifying Bmax and Kd values of [3H][D-Pen2,D-Pen5]enkephalin binding, respectively. Tritium 179-181 opioid receptor, delta 1 Mus musculus 48-82 9342225-7 1997 Functional impairment of the sulfate transporter was demonstrated in vitro by reduced incorporation of [35S]sulfate relative to [3H]glucosamine in proteoglycans synthesized by chondrocytes and by sulfate-uptake assays in fibroblasts. Tritium 129-131 solute carrier family 26 member 2 Homo sapiens 29-48 9275209-4 1997 When the injected lipoprotein had been labeled with [3H]cholesterol, the t1/2 of labeled cholesterol loss from the muscle was about 4 days in controls and more than 7 days in apo A-I K-O mice. Tritium 53-55 apolipoprotein A-I Mus musculus 175-182 9275209-8 1997 A significantly lower efflux of [3H]cholesterol from macrophages was found with native and delipidated serum from apo A-I K-O mice. Tritium 33-35 apolipoprotein A-I Mus musculus 114-121 9275223-0 1997 Autoradiographic characterization of [3H]-5-HT-moduline binding sites in rodent brain and their relationship to 5-HT1B receptors. Tritium 38-40 5-hydroxytryptamine receptor 1B Rattus norvegicus 112-118 9282933-0 1997 Cations affect [3H]mazindol and [3H]WIN 35,428 binding to the human dopamine transporter in a similar fashion. Tritium 16-18 solute carrier family 6 member 3 Homo sapiens 68-88 9282933-0 1997 Cations affect [3H]mazindol and [3H]WIN 35,428 binding to the human dopamine transporter in a similar fashion. Tritium 33-35 solute carrier family 6 member 3 Homo sapiens 68-88 9268064-6 1997 In rat cortical membranes, the B(max) value from [3H]-mesulergine binding was ten-fold lower than 5-HT2C-LI levels determined by radioimmunoassay, which may reflect measurement of internalised receptor protein by radioimmunoassay which is not detected with conventional 5-HT2C ligands. Tritium 50-52 5-hydroxytryptamine receptor 2C Rattus norvegicus 270-276 9270020-7 1997 L-MTX competitively inhibited the ATP-dependent uptake of [3H]2,4-dinitrophenyl-S-glutathione, a typical substrate for cMOAT, and the inhibition constant (Ki) of L-MTX was comparable with its own Km. Tritium 59-61 ATP binding cassette subfamily C member 2 Rattus norvegicus 119-124 9270020-9 1997 The inhibitory effects of L-MTX and its optical isomer, (-) amethopterin (D-MTX), on the uptake of [3H]L-MTX differed with Kis of 326 and 93 microM, respectively, indicating that the biologically inactive D form has a higher affinity for cMOAT than L-MTX. Tritium 100-102 ATP binding cassette subfamily C member 2 Rattus norvegicus 238-243 9277444-7 1997 IFN-gamma (300 U/ml), TNF-alpha (20 ng/ml), and IL-1 alpha (20 ng/ml) all induced a significantly increased release of prelabeled [3H]AA after 15 min to 2 h of treatment in control cells, and their effects were significantly reduced in cells transfected with cPLA2 antisense vector. Tritium 131-133 phospholipase A2 group IVA Homo sapiens 259-264 9211886-12 1997 In addition, sodium vanadate, also an inhibitor of protein phosphatase, stimulates the increase in the incorporation of [3H]acetate into acyl[3H]acetyl-GPC of the ATP-treated endothelial cells. Tritium 121-123 glycophorin C Bos taurus 152-155 9259457-1 1997 The binding sites of [3H]nociceptin (also named Orphanin FQ), the endogenous ligand of the ORL1 (opiate receptor like 1) receptor, were localized in the central nervous system of the mouse using an autoradiographic procedure. Tritium 21-24 prepronociceptin Mus musculus 48-59 9259457-1 1997 The binding sites of [3H]nociceptin (also named Orphanin FQ), the endogenous ligand of the ORL1 (opiate receptor like 1) receptor, were localized in the central nervous system of the mouse using an autoradiographic procedure. Tritium 21-24 opioid receptor-like 1 Mus musculus 91-95 9254883-6 1997 Incubation at low density (12500 cells/2 cm2) in serum-free medium revealed that clones overexpressing p185neu-T had a higher rate of [3H]thymidine incorporation than did control clones on 3, 5, and 7 d after plating (P < or = 0.0001) and constitutively overexpressed the 2.6-kb ornithine decarboxylase transcript. Tritium 135-137 erb-b2 receptor tyrosine kinase 2 Rattus norvegicus 103-110 9211490-7 1997 HGF induced dose-dependent [3H]thymidine incorporation, reaching 320, 210, and 180% above unstimulated controls in AR4-2J, PancTu-1, and 818/4 cells, respectively. Tritium 28-30 hepatocyte growth factor Rattus norvegicus 0-3 9211490-11 1997 The addition of tyrphostin, a specific tyrosine kinase inhibitor, prevented c-fos induction and inhibited HGF-induced [3H]thymidine incorporation. Tritium 119-121 hepatocyte growth factor Rattus norvegicus 106-109 9195910-5 1997 The 10-min uptake of [3H]glycerol was increased significantly (range of 4.5-8-fold over control) in oocytes expressing untagged AQP3 (GLIP) and each of the four tagged AQP3 constructs; [3H]glycerol uptake was not increased in oocytes expressing AQP1, AQP2, AQP4, AQP5, or MIP. Tritium 22-24 aquaporin 4 L homeolog Xenopus laevis 257-261 9208128-10 1997 In normal sodium diet rats, CIC 10(-4) M or ANF 10(-6) M alone inhibited angiotensin II 10(-6) M (AII)-induced decrease in intracapillary volume reflected by the glomerular [3H]-inulin space (GIS). Tritium 174-176 natriuretic peptide A Rattus norvegicus 44-47 9241534-7 1997 [3H]Thymidine incorporation studies on BMC cultured in the presence of galectin-3 demonstrated suppression of GM-CSF-induced proliferation by galectin-3. Tritium 1-3 colony stimulating factor 2 Rattus norvegicus 110-116 9145302-8 1997 Binding experiments with [3H]-corticosterone demonstrated the presence of not only GR but also mineralocorticoid receptor (MR): the dissociation constants (Kd) and the number of binding sites (Bmax) were 0.8 nM and 15 fmol/mg protein for MR and 5.0 nM and 73 fmol/mg protein for GR, respectively. Tritium 26-28 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 83-85 9087523-1 1997 Labeling of the two more important gamma-aminobutyric acidA (GABA(A)) supramolecular sites with [3H] muscimol (GABA(A)) and [3H] flunitrazepam (benzodiazepine) provided saturable, stable, and dimorphic binding activities in cortical and limbic regions of the wood mouse Apodemus sylvaticus. Tritium 97-99 gamma-aminobutyric acid (GABA) A receptor, subunit gamma 1 Mus musculus 35-68 9087523-1 1997 Labeling of the two more important gamma-aminobutyric acidA (GABA(A)) supramolecular sites with [3H] muscimol (GABA(A)) and [3H] flunitrazepam (benzodiazepine) provided saturable, stable, and dimorphic binding activities in cortical and limbic regions of the wood mouse Apodemus sylvaticus. Tritium 97-99 gamma-aminobutyric acid (GABA) A receptor, subunit gamma 1 Mus musculus 61-68 9087523-1 1997 Labeling of the two more important gamma-aminobutyric acidA (GABA(A)) supramolecular sites with [3H] muscimol (GABA(A)) and [3H] flunitrazepam (benzodiazepine) provided saturable, stable, and dimorphic binding activities in cortical and limbic regions of the wood mouse Apodemus sylvaticus. Tritium 125-127 gamma-aminobutyric acid (GABA) A receptor, subunit gamma 1 Mus musculus 35-68 9087523-1 1997 Labeling of the two more important gamma-aminobutyric acidA (GABA(A)) supramolecular sites with [3H] muscimol (GABA(A)) and [3H] flunitrazepam (benzodiazepine) provided saturable, stable, and dimorphic binding activities in cortical and limbic regions of the wood mouse Apodemus sylvaticus. Tritium 125-127 gamma-aminobutyric acid (GABA) A receptor, subunit gamma 1 Mus musculus 61-68 9130251-4 1997 Incubation of [3H]-inositol pre-labeled Schwann cells with ET-1, ET-3 or sarafotoxin 6c elicited a concentration-dependent increase in the release of [P1 that reached a plateau at approximately 100 nM. Tritium 15-17 endothelin 3 Homo sapiens 65-69 9225278-0 1997 Differential distribution of [3H]sumatriptan binding sites (5-HT1B, 5-HT1D and 5-HT1F receptors) in human brain: focus on brainstem and spinal cord. Tritium 30-32 5-hydroxytryptamine receptor 1D Homo sapiens 68-74 9099816-3 1997 The largest increase in [3H]PK-11195 binding (> 500% above control) was found in the CA3 subfield of the hippocampus. Tritium 25-27 carbonic anhydrase 3 Rattus norvegicus 88-91 9098555-7 1997 [3H]PDBu binds preferentially to PKC in the membrane and, therefore, these results suggest that stress induces the translocation of PKC from its resting compartments in the cytosol to the membrane. Tritium 1-3 protein kinase C, gamma Rattus norvegicus 33-36 9098555-7 1997 [3H]PDBu binds preferentially to PKC in the membrane and, therefore, these results suggest that stress induces the translocation of PKC from its resting compartments in the cytosol to the membrane. Tritium 1-3 protein kinase C, gamma Rattus norvegicus 132-135 9050849-8 1997 Cytohesin-1 enhanced binding of 35S-labeled guanosine 5"-[gamma-thio]triphosphate [35S]GTP[gammaS] or [3H]GDP to ARF purified from bovine brain (i.e., it appeared to function as an ARF-GEP). Tritium 103-105 cytohesin 1 Bos taurus 0-11 7589529-5 1995 P-Glycoprotein activity is evident in vitro, with resistance modulators, e.g. verapamil, producing increases in intracellular [3H]vincristine accumulation. Tritium 127-129 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 0-14 8564258-14 1995 ET-1 also stimulated a 3 fold increase in [3H]-thymidine incorporation which was abolished by pertussis toxin pretreatment. Tritium 43-45 endothelin 1 Bos taurus 0-4 7664666-5 1995 Pro-TRH was radiolabeled using [3H]Leu in AtT20 cells transfected with prepro-TRH complementary DNA, and the labeled 26-kDa pro-TRH was isolated from the cell extract by preparative sodium dodecyl sulfate-gel electrophoresis. Tritium 32-34 thyrotropin releasing hormone Mus musculus 0-7 8532076-3 1995 The K(+)-evoked tritium overflow, which was Ca(2+)-dependent but tetrodotoxin-resistant, was concentration-dependently inhibited by the nonselective 5-HT 1D alpha/1D beta receptor agonist, 5-carboxamidotryptamine. Tritium 16-23 5-hydroxytryptamine receptor 1D Homo sapiens 149-162 8584276-3 1995 The highest level of [3H]-PK11195 binding in the TMT-exposed rats occurred in the CA3/CA4 subfield of the hippocampus, followed by the primary olfactory cortex, the posteriomedial cortical amygdaloid nucleus, subiculum, and entorhinal cortex. Tritium 22-24 carbonic anhydrase 3 Rattus norvegicus 82-85 8584276-7 1995 The enhanced binding of [3H]-PK11195 in TMT-exposed rats was the result of a significant increase (p < 0.005) in the number of PBR with no change in affinity. Tritium 25-27 translocator protein Rattus norvegicus 130-133 8584276-9 1995 These findings suggest that the quantitative assessment of [3H]-PK11195 binding to PBR in the brain could represent a potential biomarker for assessing chemical-induced neurotoxicity. Tritium 60-62 translocator protein Rattus norvegicus 83-86 9435202-2 1998 Ang II and Ang-(1-7) enhanced AA release in cells prelabeled with [3H]AA. Tritium 67-69 angiogenin Oryctolagus cuniculus 11-14 7642558-7 1995 10 microM CNP increased osteoclast bone resorptive activity, measured by the resorption area on whale dentine wafers, or by the NH4Cl-inhibitable release of [3H]proline from radiolabeled bone chips, to 214 and 557% of control, respectively, without affecting osteoclast formation. Tritium 158-160 natriuretic peptide type C Mus musculus 10-13 9082939-2 1997 METHODS: The reactivity pattern of hsp65-reactive T cell clones generated under limiting-dilution conditions was analyzed in 3H-thymidine incorporation assays. Tritium 125-127 heat shock protein family D (Hsp60) member 1 Homo sapiens 35-40 9435202-5 1998 Angiotensin type (AT)1 receptor antagonist DUP-753 inhibited only Ang II-induced [3H]AA release, whereas the AT2 receptor antagonist PD-123319 inhibited both Ang II- and Ang-(1-7)-induced [3H]AA release. Tritium 82-84 angiogenin Oryctolagus cuniculus 66-69 9134216-5 1997 The expression of either P2Y1 or P2Y2 receptors in 1321N1 human astrocytoma cells results, on a change of medium, in an elevation of basal (no added agonist) accumulation of [3H]-inositol(poly)phosphates([3H]-InsPx) compared to cells not expressing these receptors. Tritium 175-177 purinergic receptor P2Y2 Homo sapiens 33-37 7631152-6 1995 Unstimulated thymocytes or their supernatant caused 40-80% inhibition of TEC cell proliferation, as measured by 3H-thymidine incorporation. Tritium 112-114 tec protein tyrosine kinase Mus musculus 73-76 9134216-5 1997 The expression of either P2Y1 or P2Y2 receptors in 1321N1 human astrocytoma cells results, on a change of medium, in an elevation of basal (no added agonist) accumulation of [3H]-inositol(poly)phosphates([3H]-InsPx) compared to cells not expressing these receptors. Tritium 205-207 purinergic receptor P2Y2 Homo sapiens 33-37 9435202-5 1998 Angiotensin type (AT)1 receptor antagonist DUP-753 inhibited only Ang II-induced [3H]AA release, whereas the AT2 receptor antagonist PD-123319 inhibited both Ang II- and Ang-(1-7)-induced [3H]AA release. Tritium 82-84 angiogenin Oryctolagus cuniculus 66-69 8789288-2 1995 IL-10 inhibited the spontaneous proliferation in 1 out of 12 (1/12) cases while the IL-1 stimulated the tritiated thymidine (3H-TdR) uptake was suppressed in 2/12 cases as a result of IL-10 administration. Tritium 125-127 interleukin 10 Homo sapiens 184-189 9435202-8 1998 The CaM kinase II inhibitor KN-93 and the MAP kinase kinase inhibitor PD-98059 attenuated both Ang-(1-7)- and Ang II-induced cPLA2 activity and [3H]AA release. Tritium 145-147 angiogenin Oryctolagus cuniculus 110-113 8789288-3 1995 In the presence of G-CSF, IL-10 affected 3H-TdR uptake in 2/12 and no distinct changes were observed in the presence of IL-3. Tritium 41-43 interleukin 10 Homo sapiens 26-31 9231352-5 1997 In 24-month-old rats, however, [3H]nimodipine binding increased significantly in the striatum and hippocampal CA3 sector. Tritium 31-34 carbonic anhydrase 3 Rattus norvegicus 110-113 9434132-2 1997 Expression of this receptor was demonstrated with specific binding of the mu-opioid selective ligand [3H][D-Ala2,N-MePhe4,Gly5-ol]-enkephalin ([3H]DAMGO). Tritium 144-146 proenkephalin Rattus norvegicus 131-141 7564919-4 1995 We find that both GM-CSF and SLF induced increased phosphatidylcholine (PC) turnover rates (biosynthesis and degradation) as measured by increased [3H]-choline labelling, with SLF being more potent than GM-CSF after 6 h of stimulation, but equipotent at 24 h of stimulation. Tritium 148-150 KIT ligand Homo sapiens 29-32 9417838-5 1997 IFN-gamma significantly inhibited the proliferation of rat cortical astrocytes both in serum-free and serum-containing media as measured by [3H]thymidine incorporation. Tritium 141-143 interferon gamma Rattus norvegicus 0-9 7794908-5 1995 When cholesterol-enriched mouse macrophages were exposed to lipid-free apo AI at 20 micrograms/mL (706 nM), there was significant efflux of [14C]cholesterol and [3H]phospholipid (20% +/- 0.5%/24 h and 6% +/- 0.3%/24 h, respectively). Tritium 162-164 apolipoprotein A-I Mus musculus 71-77 7786891-4 1995 In order to examine the mechanistic basis underlying the inhibitory action of 15-HETE, association assays of [3H]-15-HETE with RBL-1 subcellular fractions were carried out. Tritium 110-112 RB transcriptional corepressor like 1 Rattus norvegicus 127-132 7582477-8 1995 Distribution of PKC was determined by [3H]-phorbol-12,13-dibutyrate ([3H]-PDBu) binding and PKC activity by the Amersham enzyme assay system. Tritium 39-41 protein kinase C, gamma Rattus norvegicus 16-19 7582477-8 1995 Distribution of PKC was determined by [3H]-phorbol-12,13-dibutyrate ([3H]-PDBu) binding and PKC activity by the Amersham enzyme assay system. Tritium 70-72 protein kinase C, gamma Rattus norvegicus 16-19 9044380-8 1997 The results, based on these pharmacological profiles, suggest the possible involvement of alpha 3 and beta 2 nicotinic acetylcholine receptor subunits in the control of [3H]noradrenaline release from hippocampal slices. Tritium 170-172 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 109-141 9068081-6 1997 PAF-AH activity was assessed by measuring the percentage of 3H-labeled PAF hydrolyzed. Tritium 60-62 phospholipase A2 group VII Homo sapiens 0-6 9070858-5 1997 Over-expression of human Edg2 in CHO cells correlated with increases in specific binding of [3H]-LPA. Tritium 93-95 lysophosphatidic acid receptor 1 Homo sapiens 25-29 9458266-8 1997 In contrast, the rate of appearance of LDL-derived free [3H]cholesterol in the plasma membrane of NP-C subjects, as detected in plasma and bile, was retarded to a similar extent that LDL cholesterol metabolism was defective in cultured fibroblasts of these affected subjects. Tritium 57-59 NPC intracellular cholesterol transporter 1 Homo sapiens 98-102 9038367-2 1997 [3H]Nociceptin/orphanin FQ (OFQ) bound to the cell membrane specifically (Kd = 3.6 +/- 0.6 nM) and inhibited forskolin-stimulated cAMP accumulation (EC50 = 0.72 +/- 0.3 nM). Tritium 1-3 prepronociceptin Mus musculus 15-26 9068340-2 1997 We have determined the effects of halothane on the translocation and down-regulation of conventional PKC (cPKC) by analysing the subcellular distribution of PKC activity, [3H]phorbol-12,13-dibutyrate ([3H]PDBu) binding and PKC immunoreactivity in intact rat cerebrocortical synaptosomes, a subcellular fraction that contains functional nerve terminals. Tritium 202-204 protein kinase C, alpha Rattus norvegicus 101-104 7539396-4 1995 Prostaglandin E1 (PGE1), PGE2, and PGF2 alpha induced DNA synthesis even at such a low concentration as 5 nmol/L and potentiated [3H]thymidine incorporation induced by HGF in an additive manner. Tritium 130-132 hepatocyte growth factor Rattus norvegicus 168-171 9361897-4 1997 RESULTS: Epidermal growth factor was a potent mitogen in canine and equine MC, stimulating dose-dependent increases in [3H]thymidine incorporation and moderate increase in cell numbers. Tritium 120-122 epidermal growth factor Canis lupus familiaris 9-32 7791118-4 1995 The pharmacology of [3H]ABT-418 binding was consistent with an interaction with the putative alpha 4 beta 2 nAChR subtype. Tritium 21-23 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 108-113 7791118-5 1995 The nAChR agonists, (-)-nicotine, (-)-cytisine and (+/-)-epibatidine, displayed a high affinity (Ki = 0.8 +/- 0.1, 0.2 +/- 0.1 and 0.05 +/- 0.01 nM, respectively) for [3H]ABT-418 binding sites, whereas among nAChR antagonists examined, only dihydro-beta-erythroidine competed with high affinity (Ki = 32 +/- 1.5 nM). Tritium 168-170 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 4-9 9023275-1 1997 The effects of selective nicotinic acetylcholine (ACh) receptor (nAChR) agonists and antagonists on the stimulation-evoked release of [3H]ACh were studied in rat isolated superior cervical ganglion loaded with [3H]choline and superfused in a 2-ml chamber. Tritium 135-137 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 65-70 9361897-5 1997 Although PDGF alone did not significantly stimulate [3H]thymidine incorporation in canine MC, PDGF was synergistic with EGF in stimulation of [3H]thymidine incorporation in canine and equine MC, and PDGF significantly stimulated [3H]thymidine incorporation in equine MC. Tritium 143-145 epidermal growth factor Canis lupus familiaris 120-123 9135047-3 1997 Unilateral intranigral administration of dopamine transporter antisense (50 microM) twice daily in freely moving rats for 2.5 days was sufficient to reduce dopamine transporter messenger RNA by 70% as measured by in situ hybridization, but not protein levels as measured by [3H]mazindol binding. Tritium 275-277 solute carrier family 6 member 3 Homo sapiens 41-61 9361897-5 1997 Although PDGF alone did not significantly stimulate [3H]thymidine incorporation in canine MC, PDGF was synergistic with EGF in stimulation of [3H]thymidine incorporation in canine and equine MC, and PDGF significantly stimulated [3H]thymidine incorporation in equine MC. Tritium 143-145 epidermal growth factor Canis lupus familiaris 120-123 7603461-6 1995 Gluc(2,3",4")P3 specifically displaced [3H]Ins(1,4,5)P3 from pig cerebellar Ins(1,4,5)P3 binding sites, with an affinity (IC50 = 130 nM) only 5-fold weaker than that of Ins(1,4,5)P3 (IC50 = 27 nM). Tritium 40-42 glucosylceramidase beta 3 (gene/pseudogene) Homo sapiens 0-4 9385373-4 1997 The gene was mapped by fluorescence in situ hybridization and by in situ hybridization with a [3H]-labelled DNA probe to chromosome 22q11.2, a region previously implicated in type-I hyperprolinaemia in a case of CATCH 22 syndrome, a contiguous gene deletion syndrome involving 22q11. Tritium 95-97 DiGeorge syndrome chromosome region Homo sapiens 212-220 7644026-1 1995 By means of autoradiography we have studied the cellular localization of binding sites for [3H]neurotensin and its nonpeptide receptor antagonist [3H]SR-48692 in explant cultures of rat neocortex, striatum, brain stem and spinal cord. Tritium 92-94 neurotensin Rattus norvegicus 95-106 7644026-5 1995 Binding of [3H]neurotensin and [3H]SR-48692 (10(-8)M) to neurons and glial cells was markedly reduced or inhibited by the unlabelled compounds at high concentration (10(-6)M), suggesting "specific" binding of the radioligands. Tritium 12-14 neurotensin Rattus norvegicus 15-26 7552274-2 1995 In the dorsal hippocampus, [3H]YM-09151-2 bindings were high in the whole layers of CA1, the stratum pyramidale of CA4 and the stratum molecular of gyrus dentatus, moderate in the stratum oriens of CA3 and hilus of the gyrus dentatus, and low in remaining CA3 and the subiculum. Tritium 28-30 carbonic anhydrase 3 Rattus norvegicus 198-201 9131886-1 1997 Rat neuronal nitric oxide (NO) synthase (nNOS) activity was measured in frontal cortex, hippocampus, striatum and cerebellum using the assay of [3H]citrulline, following chronic alcoholization. Tritium 145-147 nitric oxide synthase 1 Rattus norvegicus 41-45 9117086-2 1997 The degradation of bradykinin (BK) labelled with tritiated proline at positions 2 and 3 ([3H]-BK) was determined on the luminal surface of bovine tracheal epithelium, in supernatants obtained from incubations of the luminal tracheal surface, and in suspensions of isolated tracheal epithelial cells. Tritium 90-92 kininogen 1 Bos taurus 19-29 9117086-2 1997 The degradation of bradykinin (BK) labelled with tritiated proline at positions 2 and 3 ([3H]-BK) was determined on the luminal surface of bovine tracheal epithelium, in supernatants obtained from incubations of the luminal tracheal surface, and in suspensions of isolated tracheal epithelial cells. Tritium 90-92 kininogen 1 Bos taurus 31-33 7552274-2 1995 In the dorsal hippocampus, [3H]YM-09151-2 bindings were high in the whole layers of CA1, the stratum pyramidale of CA4 and the stratum molecular of gyrus dentatus, moderate in the stratum oriens of CA3 and hilus of the gyrus dentatus, and low in remaining CA3 and the subiculum. Tritium 28-30 carbonic anhydrase 3 Rattus norvegicus 256-259 7762684-5 1995 Quantitative in situ hybridization with 3H-labeled cRNA probes showed both IL-8 and MCP-1 mRNA expression in AM during oxygen exposure, with peak levels of IL-8 mRNA at 56-h oxygen exposure and of MCP-1 mRNA at 64-h oxygen exposure with 24-h room air recovery. Tritium 40-42 C-C motif chemokine 2 Oryctolagus cuniculus 84-89 7746272-0 1995 [3H]SR 48692, the first nonpeptide neurotensin antagonist radioligand: characterization of binding properties and evidence for distinct agonist and antagonist binding domains on the rat neurotensin receptor. Tritium 1-3 neurotensin Rattus norvegicus 35-46 9117086-2 1997 The degradation of bradykinin (BK) labelled with tritiated proline at positions 2 and 3 ([3H]-BK) was determined on the luminal surface of bovine tracheal epithelium, in supernatants obtained from incubations of the luminal tracheal surface, and in suspensions of isolated tracheal epithelial cells. Tritium 90-92 kininogen 1 Bos taurus 94-96 9117086-7 1997 Metabolism of [3H]-BK was unaltered in the presence of ramiprilat (250 nM) or phosphoramidon (10 microM). Tritium 15-17 kininogen 1 Bos taurus 19-21 9117086-16 1997 Degradation of [3H]-BK was not influenced by ramiprilat, but was inhibited by 85% in the presence of phosphoramidon. Tritium 16-18 kininogen 1 Bos taurus 20-22 8977379-6 1997 Scatchard analysis of [3H]1,25-(OH)2D3 binding showed the VDR concentration to be 173 fmol/mg protein and the affinity to be 0.12 nM. Tritium 23-25 vitamin D (1,25-dihydroxyvitamin D3) receptor Mus musculus 58-61 7617701-4 1995 Mechanically induced increases in activity by forced running stress results in a significant reduction in [3H]Ro 5-4864 binding to PBR in olfactory bulb, opposite to the PBR changes in this tissue following forced cold-water swim stress. Tritium 107-109 translocator protein Homo sapiens 131-134 9317158-4 1997 Sublytic C5b-9 markedly increased free [3H]AA in cPLA2-wt-transfected GEC, but only trivial increases were evident in secretory PLA2-transfected, or neo (control) GEC. Tritium 40-42 phospholipase A2 group IVA Rattus norvegicus 49-54 7702575-2 1995 YC-1 (1-100 microM) dose-dependently inhibited [3H]thymidine incorporation into DNA in rat A10 VSMCs that were synchronized by serum depletion and then restimulated by addition of 10% foetal calf serum (FCS), whereas FCS-induced [3H]thymidine incorporation into rat synchronized endothelial cells was unaffected by this agent. Tritium 230-232 glutathione S-transferase alpha 1 Rattus norvegicus 0-4 7606435-3 1995 The highest concentrations of [3H]WAY-100635-labelled sites were found in the lateral septal areas, dorsal raphe n., entorhinal cortex and the hippocampal formation (CA1, CA3 and dentate gyrus). Tritium 31-33 carbonic anhydrase 3 Rattus norvegicus 171-174 8978354-6 1997 RESULTS: In colitis, both topical budesonide and systemic IL-1ra treatments attenuated the suppression of KCl-evoked 3H release from longitudinal muscle myenteric plexus in both inflamed and noninflamed segments. Tritium 117-119 interleukin 1 receptor antagonist Rattus norvegicus 58-64 9039119-13 1997 Consistent with the results observed with [3H]-thymidine uptake and radioligand binding assays, the expression of thromboxane A2 receptor protein was decreased in nonresponder compared with responder vascular tissue. Tritium 43-45 TBXA2R Oryctolagus cuniculus 114-137 9317158-4 1997 Sublytic C5b-9 markedly increased free [3H]AA in cPLA2-wt-transfected GEC, but only trivial increases were evident in secretory PLA2-transfected, or neo (control) GEC. Tritium 40-42 phospholipase A2 group IB Rattus norvegicus 50-54 9317158-5 1997 In cPLA2-wt-transfected GEC, reduction of extracellular free Ca2+ or down-regulation of protein kinase C inhibited [3H]AA release. Tritium 116-118 phospholipase A2 group IVA Rattus norvegicus 3-8 9317158-7 1997 In GEC that express cPLA2-deltaCaLB, the C5b-9-induced increase in free [3H]AA was comparable with neo, despite expression of cPLA2-deltaCaLB at levels similar to cPLA2-wt. Tritium 73-75 phospholipase A2 group IVA Rattus norvegicus 20-25 7890504-8 1995 MIB-1 labeling yields an approximate fivefold increase over the number of proliferating cells detected in retinal sections using 3H-thymidine autoradiography. Tritium 129-131 E3 ubiquitin-protein ligase MIB1 Oryctolagus cuniculus 0-5 9317158-10 1997 At low extracellular free Ca2+, C5b-9 increased free [3H]AA significantly in GEC that express cPLA2-srcmyr, while in neo GEC, the change was negligible. Tritium 54-56 phospholipase A2 group IVA Rattus norvegicus 94-99 9395250-4 1997 Cells that overexpressed prothymosin alpha increased the proliferative activity assayed by the [3H]-thymidine incorporation or by the cell cycle analysis with the fluorescent-activated cell sorter. Tritium 96-98 prothymosin alpha Mus musculus 25-42 9252235-0 1997 Histamine H3 receptor activation selectively inhibits dopamine D1 receptor-dependent [3H]GABA release from depolarization-stimulated slices of rat substantia nigra pars reticulata. Tritium 86-88 histamine receptor H3 Rattus norvegicus 0-21 9048963-2 1997 We characterized [3H]BFI binding in human brain cortex and lateral reticular nucleus (NRL). Tritium 18-20 neural retina leucine zipper Homo sapiens 86-89 9048963-13 1997 Thus, [3H]BFI labels a site in human NRL and cortex that appears similar to the previously described I2 site labeled by [3H]idazoxan. Tritium 7-9 neural retina leucine zipper Homo sapiens 37-40 8985148-4 1996 Lowering the growth temperature, and reducing the amount of inducer, resulted in the production of soluble LO, which was active on a degrees [3H]lysine-labeled elastin substrate. Tritium 142-144 elastin Homo sapiens 160-167 7776977-4 1995 Corresponding [3H]thymidine incorporation experiments show that the maximum level of c15 mRNA expression correlates with the G1/S transition phase of the cell cycle. Tritium 15-17 nuclear distribution C, dynein complex regulator Rattus norvegicus 85-88 7836415-2 1995 COS-7 cells transfected with a PKD cDNA construct (pcDNA3-PKD) exhibit a marked (4.8-fold) increase in [3H]phorbol 12,13-dibutyrate binding. Tritium 104-106 protein kinase D1 Mus musculus 31-34 7836415-2 1995 COS-7 cells transfected with a PKD cDNA construct (pcDNA3-PKD) exhibit a marked (4.8-fold) increase in [3H]phorbol 12,13-dibutyrate binding. Tritium 104-106 protein kinase D1 Mus musculus 58-61 9252235-2 1997 The histamine H3 receptor-selective agonist immepip (1 microM) and the non-selective agonist histamine (100 microM) inhibited [3H]GABA release by 78 +/- 2 and 80 +/- 2%, respectively. Tritium 127-129 histamine receptor H3 Rattus norvegicus 4-25 9238062-2 1997 Coexpression of either rat gamma2 or gamma3, in combination with alpha1 and beta2 subunits, results both in receptors displaying high [3H]Ro 15-1788 affinity. Tritium 135-137 adrenoceptor alpha 1D Homo sapiens 65-71 7775392-4 1995 Degradation of [3H]elastin by rMME absolutely required Ca2+; the optimal Ca2+ concentration was 5 mM. Tritium 16-18 membrane metallo-endopeptidase Rattus norvegicus 30-34 9260907-2 1997 Here we report on retinol uptake through the RBP-TTR complex by primary cultured rat hepatocytes (parenchymal cells, PC) and nonparenchymal cells (NPC) following incubation with [3H]retinol-RBP or the [3H]retinol-RBP-TTR complex under several conditions. Tritium 179-181 retinol binding protein 4 Rattus norvegicus 45-48 8587388-5 1995 In the presence of phosphoramidon, ET-1 and ET-3 equipotently stimulated inositol 1,4,5-trisphosphate formation and [3H]-thymidine incorporation into cultured ECs. Tritium 117-119 endothelin 1 Bos taurus 35-39 8954936-5 1996 When functionally expressed in HeLa cells, the hSigmaR1 cDNA enhances the binding of [3H]-haloperidol, a sigma receptor ligand, to the HeLa cell membranes. Tritium 86-88 sigma non-opioid intracellular receptor 1 Homo sapiens 47-55 8968107-9 1996 Exogenous IL-10, IL-6, IL-2, and TNF-alpha significantly enhanced the [3H]thymidine uptake in 13 of 13 (100%), 5 of 13 (38%), 9 of 13 (69%), and 2 of 10 (20%) PTCPs costimulated with anti-CD40, respectively. Tritium 71-73 interleukin 10 Homo sapiens 10-15 9034933-5 1996 The activity of [3H]-leucine incorporated into albumin synthesis, an indicator of translational activity, was higher on days 5 and 10 than on day 2 and was highest on day 5. Tritium 17-19 albumin Rattus norvegicus 47-54 7798945-2 1995 The main and earliest formed 3H-metabolites of [Sph-3H]GM1 were GM2, asialo-GM1 asialo-GM2, and lactose-ceramide, and those of [Sph-3H]GM2 were asialo-GM2 and lactose-ceramide. Tritium 29-31 coenzyme Q10A Mus musculus 55-58 7798945-2 1995 The main and earliest formed 3H-metabolites of [Sph-3H]GM1 were GM2, asialo-GM1 asialo-GM2, and lactose-ceramide, and those of [Sph-3H]GM2 were asialo-GM2 and lactose-ceramide. Tritium 29-31 coenzyme Q10A Mus musculus 76-79 8748677-2 1995 Saturation experiments with the D2 agonist [3H]NPA (N-propylnorapomorphine) showed that 10 nM of NT, 10 nM of neuromedin N (NN) and 1 nM of the C-terminal NT-(8-13) fragment significantly increased the KD values by 125%, 181%, and 194%, respectively without significantly affecting the Bmax value of the [3H]NPA binding sites in coronal sections of rat ventral forebrain mainly containing the nucleus accumbens (Acb) and the olfactory tubercle. Tritium 44-46 neurotensin Rattus norvegicus 97-99 8748677-2 1995 Saturation experiments with the D2 agonist [3H]NPA (N-propylnorapomorphine) showed that 10 nM of NT, 10 nM of neuromedin N (NN) and 1 nM of the C-terminal NT-(8-13) fragment significantly increased the KD values by 125%, 181%, and 194%, respectively without significantly affecting the Bmax value of the [3H]NPA binding sites in coronal sections of rat ventral forebrain mainly containing the nucleus accumbens (Acb) and the olfactory tubercle. Tritium 44-46 neurotensin Rattus norvegicus 110-122 8748677-2 1995 Saturation experiments with the D2 agonist [3H]NPA (N-propylnorapomorphine) showed that 10 nM of NT, 10 nM of neuromedin N (NN) and 1 nM of the C-terminal NT-(8-13) fragment significantly increased the KD values by 125%, 181%, and 194%, respectively without significantly affecting the Bmax value of the [3H]NPA binding sites in coronal sections of rat ventral forebrain mainly containing the nucleus accumbens (Acb) and the olfactory tubercle. Tritium 305-307 neurotensin Rattus norvegicus 155-157 8931474-6 1996 The in vivo release of the selective CCK-B agonist pBC 264 (associated with a tracer dose of [3H]pBC 264) from microspheres prepared with OVA was very fast (92% after 6 h), whereas only 26% (88 pmol) of pBC 264 was released from the formulation with Pluronic F 68 after 24 h. Eight days after implantation 36% of pBC 264 had diffused from the microspheres, and 8% (approximately 30 pmol) was still present in the brain concentrated around the site of administration. Tritium 94-96 cholecystokinin Rattus norvegicus 37-40 9260907-2 1997 Here we report on retinol uptake through the RBP-TTR complex by primary cultured rat hepatocytes (parenchymal cells, PC) and nonparenchymal cells (NPC) following incubation with [3H]retinol-RBP or the [3H]retinol-RBP-TTR complex under several conditions. Tritium 202-204 retinol binding protein 4 Rattus norvegicus 45-48 7475979-2 1995 The cloned receptor is expressed at a reasonably high density (0.82 fmole/microgram protein) and exhibits high affinity for [3H]OT (Kd = 0.32nM), similar to the value found in human myometrial tissue. Tritium 125-127 oxytocin/neurophysin I prepropeptide Homo sapiens 128-130 9260907-6 1997 The uptake of [3H]retinol from protein-bound retinol was inhibited by an excess of either retinol-RBP or retinol-RBP-TTR complex. Tritium 15-17 retinol binding protein 4 Rattus norvegicus 98-101 7475979-3 1995 The rank-order of potency for various antagonist and agonist ligands from several structural classes is also similar between the cloned and native receptor, as seen in a comparison of their inhibitory constants for [3H]OT binding. Tritium 216-218 oxytocin/neurophysin I prepropeptide Homo sapiens 219-221 8970973-6 1996 We report that a phosphorothioate-modified antisense oligonucleotide specific for cPLA2, but not the control oligonucleotide, inhibited the TNF-induced release of both [3H]arachidonic acid and 51Cr from infected cells. Tritium 169-172 phospholipase A2 group IVA Homo sapiens 82-87 9260907-6 1997 The uptake of [3H]retinol from protein-bound retinol was inhibited by an excess of either retinol-RBP or retinol-RBP-TTR complex. Tritium 15-17 retinol binding protein 4 Rattus norvegicus 113-116 8910465-8 1996 Functionally, exposure of smooth muscle cells or endothelial cells to Factor Xa induced a 3-fold and a 2-fold increase in [3H]thymidine uptake, respectively. Tritium 123-125 coagulation factor X Homo sapiens 70-79 9235900-10 1997 Interestingly, IGF-I stimulates incorporation of [3H]phenylalanine (1.6-fold) without any effect on [3H]thymidine incorporation. Tritium 50-52 insulin-like growth factor 1 Rattus norvegicus 15-20 8895748-7 1996 Introduction of a recombinant RARalpha expression vector into benign keratinocyte tumor cells reduced the S-phase population and inhibited [3H]thymidine incorporation in response to retinoic acid. Tritium 140-142 retinoic acid receptor, alpha Mus musculus 30-38 8833998-9 1995 Serum stimulation of Chinese hamster ovary (CHO) cells expressing the edg-2 receptor resulted in increased cell proliferation, as measured by [3H]-thymidine incorporation. Tritium 143-145 lysophosphatidic acid receptor 1 Homo sapiens 70-75 7802629-3 1994 Immunoprecipitation experiment of [3H]InsP4 binding activity of the purified protein using polyclonal antibody against the C2A domain of rat synaptotagmin II also revealed that mouse synaptotagmin II is the InsP4 binding protein (IP4BP). Tritium 35-37 synaptotagmin II Mus musculus 183-199 7988451-5 1994 High affinity, low capacity binding sites for [3H]estradiol (Kd, 0.11 nM; binding capacity, 6.2 fmol/mg protein) and for a ligand of the androgen receptor, [3H]R1881 (Kd, 0.054 nM; binding capacity, 9.58 fmol/mg protein), have been identified in this cell line. Tritium 157-159 androgen receptor Mus musculus 137-154 8908208-7 1996 Fluprostenol (1 microM), a PGF2 alpha receptor agonist > 17-phenyl-trinor-PGE2 (1 microM), an EP1 receptor agonist stimulated [3H]thymidine incorporation, but an EP3 receptor agonist, ONO-AP-324 nor an EP4 (EP2) receptor agonist, 11-deoxy-PGE1 (1 microM) did not. Tritium 130-132 prostaglandin F receptor Mus musculus 27-46 8908208-7 1996 Fluprostenol (1 microM), a PGF2 alpha receptor agonist > 17-phenyl-trinor-PGE2 (1 microM), an EP1 receptor agonist stimulated [3H]thymidine incorporation, but an EP3 receptor agonist, ONO-AP-324 nor an EP4 (EP2) receptor agonist, 11-deoxy-PGE1 (1 microM) did not. Tritium 130-132 prostaglandin E receptor 4 (subtype EP4) Mus musculus 205-208 7882003-3 1994 [3H]PDBu binding in stratum oriens of the CA1 and CA3 sectors and stratum lucidum of the CA3 sector increased to adult levels by 4 weeks. Tritium 1-3 carbonic anhydrase 3 Rattus norvegicus 50-53 9245713-5 1997 In this report we show that the A33 antigen is (I) N-glycosylated, containing approximately 8 K of N-linked carbohydrate and there is no evidence for O-glycosylation, sialylation or glycophosphatidylinositol, and (ii) S-acylated in vitro, incorporating [3H] palmitic acid linked through a hydroxylamine-sensitive thioester bond. Tritium 254-256 glycoprotein A33 Homo sapiens 32-35 7923639-5 1994 [3H]PDBu binding assay revealed increased PKC concentration in LVH cytosolic (control, 0.51 +/- 0.06 pmol/L per milligram; LVH, 0.78 +/- 0.09 pmol/L per milligram; n = 5; P < .05) and membrane fractions (control, 1.33 +/- 0.15; LVH, 2.32 +/- 0.39; n = 5; P < .05). Tritium 1-3 protein kinase C, alpha Rattus norvegicus 42-45 7851480-4 1994 In in vitro binding assays all three dl-threo bromo compounds had higher affinities than methylphenidate for dopamine transporter sites labeled with [3H]2 beta-carbomethoxy-3 beta-(4-fluorophenyl)tropane ([3H]WIN 35,428; IC50 = 13, 4, 20 and 82 nM for o-, m-, and p-bromo compounds, and unsubstituted methylphenidate, respectively). Tritium 150-152 solute carrier family 6 member 3 Homo sapiens 109-129 7851480-4 1994 In in vitro binding assays all three dl-threo bromo compounds had higher affinities than methylphenidate for dopamine transporter sites labeled with [3H]2 beta-carbomethoxy-3 beta-(4-fluorophenyl)tropane ([3H]WIN 35,428; IC50 = 13, 4, 20 and 82 nM for o-, m-, and p-bromo compounds, and unsubstituted methylphenidate, respectively). Tritium 206-208 solute carrier family 6 member 3 Homo sapiens 109-129 8089154-5 1994 The 39-kDa core protein is anchored to the cell surface by a glycosyl phosphatidylinositol anchor as evidenced by 1) release of the HSPG with the 39-kDa core protein into media by PIPLC treatment and 2) biosynthetic incorporation of [3H]ethanolamine and [32P]orthophosphate into the PIPLC-releasable 39-kDa core protein. Tritium 234-236 syndecan 2 Homo sapiens 132-136 8908208-7 1996 Fluprostenol (1 microM), a PGF2 alpha receptor agonist > 17-phenyl-trinor-PGE2 (1 microM), an EP1 receptor agonist stimulated [3H]thymidine incorporation, but an EP3 receptor agonist, ONO-AP-324 nor an EP4 (EP2) receptor agonist, 11-deoxy-PGE1 (1 microM) did not. Tritium 130-132 prostaglandin E receptor 2 (subtype EP2) Mus musculus 210-223 8900315-2 1996 In agreement with previous reports, CD4 mAbs of different species (mouse, rat, humanized), isotypes (IgG1, IgG2a, and IgG2b) and different epitope specificities decreased 3H-TdR incorporation in MLR, using monocyte-depleted or CD4+ T lymphocyte-enriched blood mononuclear cells as responders. Tritium 171-173 CD4 antigen Mus musculus 36-39 8900315-2 1996 In agreement with previous reports, CD4 mAbs of different species (mouse, rat, humanized), isotypes (IgG1, IgG2a, and IgG2b) and different epitope specificities decreased 3H-TdR incorporation in MLR, using monocyte-depleted or CD4+ T lymphocyte-enriched blood mononuclear cells as responders. Tritium 171-173 gamma-2a immunoglobulin heavy chain Rattus norvegicus 107-112 8900315-2 1996 In agreement with previous reports, CD4 mAbs of different species (mouse, rat, humanized), isotypes (IgG1, IgG2a, and IgG2b) and different epitope specificities decreased 3H-TdR incorporation in MLR, using monocyte-depleted or CD4+ T lymphocyte-enriched blood mononuclear cells as responders. Tritium 171-173 Cd4 molecule Rattus norvegicus 227-230 8906876-1 1996 [3H]Thymidine (TdR) incorporation by human osteosarcoma cell line MG-63 was significantly stimulated at as early as 3 h after the addition of either TIMP-1 or TIMP-2 alone. Tritium 1-3 TIMP metallopeptidase inhibitor 2 Homo sapiens 159-165 8916088-4 1996 Here we show evidence that A beta at nanomolar levels promotes cell growth determined by [3H]thymidine incorporation, protein content and cell counts. Tritium 90-92 amyloid beta precursor protein Rattus norvegicus 27-33 9224735-2 1997 In the presence of [3H]RA both RAR alpha and RXR alpha existed primarily as monomers of 50 kDa in solutions containing 80 mM KCl. Tritium 20-22 retinoic acid receptor alpha Homo sapiens 31-40 8831495-8 1996 DNA synthesis, which was monitored by measuring 5-bromo-2"-deoxyuridine (BrdU) and [3H]thymidine incorporation, was increased by twofold to threefold in IGF2-stimulated fetal ventricular myocytes, whereas no change in BrdU or [3H]thymidine incorporation was observed in neonatal ventricular myocytes. Tritium 84-86 insulin-like growth factor 2 Rattus norvegicus 153-157 8082810-3 1994 In cultures of human skin fibroblasts, TRAMP incorporates both [35S]sulphate and [3H]tyrosine and is secreted into the medium, as shown by immunoprecipitation. Tritium 82-84 dermatopontin Homo sapiens 39-44 7844157-8 1994 We also demonstrated that [3H]thymidine was significantly incorporated into Raji cells, a Burkitt lymphoma cell line, in the presence of either 4 ng/ml of TIMP-1 or 0.1 ng/ml of TIMP-2. Tritium 27-29 TIMP metallopeptidase inhibitor 2 Homo sapiens 178-184 8831495-8 1996 DNA synthesis, which was monitored by measuring 5-bromo-2"-deoxyuridine (BrdU) and [3H]thymidine incorporation, was increased by twofold to threefold in IGF2-stimulated fetal ventricular myocytes, whereas no change in BrdU or [3H]thymidine incorporation was observed in neonatal ventricular myocytes. Tritium 227-229 insulin-like growth factor 2 Rattus norvegicus 153-157 9211943-8 1997 An azido-Q-linked peptide with a retention time of 66 min is obtained by high performance liquid chromatography of the chymotrypsin digest of carboxymethylated and succinylated [3H]azido-Q-labeled QPs3 purified from labeled succinate-ubiquinone reductase by a procedure involving phenyl-Sepharose 4B column chromatography, preparative SDS-polyacrylamide gel electrophoresis, and acetone precipitation. Tritium 178-180 succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial Bos taurus 197-201 8021292-3 1994 To test this, we developed an in vitro assay in which we added [3H]-elastin to cultured vascular SMC after 24 h serum starvation and monitored elastolysis following a further 24 h incubation with fetal bovine serum (FBS). Tritium 64-66 elastin Rattus norvegicus 68-75 7921158-1 1994 Epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), and insulin all dose-dependently stimulated [3H]-thymidine incorporation into guinea pig gastric mucous cells cultured in vitro. Tritium 115-117 insulin Cavia porcellus 74-81 8888009-1 1996 The effect of 7-nitroindazole (7-NI), an inhibitor of neuronal nitric oxide synthase (nNOS) on the dimethylphenylpiperazinium(DMPP)-evoked release of [3H]noradrenaline ([3H]NA) from rat hippocampal slices was studied. Tritium 151-153 nitric oxide synthase 1 Rattus norvegicus 54-84 9246604-3 1997 PAF (1 microM) stimulates and PAF antagonists (0.1-1 microM) inhibit [3H]thymidine incorporation in cells grown in 5% serum. Tritium 70-72 PCNA clamp associated factor Homo sapiens 0-3 8888009-7 1996 Our data show that 7-NI influences the carrier-mediated component of DMPP-evoked [3H]NA release, which indicates that nitric oxide produced by nNOS may play a role in the regulation of the NA uptake carrier. Tritium 82-84 nitric oxide synthase 1 Rattus norvegicus 143-147 8207430-3 1994 The inhibitory effect of CADO (5 microM) on [3H]ACh release was prevented by the A1 antagonist 1,3-dipropyl-8-cyclopentylxanthine (DPCPX; 50 nM) in the three hippocampal areas and was converted in an excitatory effect in the CA3 and dentate gyrus areas. Tritium 45-47 carbonic anhydrase 3 Rattus norvegicus 225-228 9246604-3 1997 PAF (1 microM) stimulates and PAF antagonists (0.1-1 microM) inhibit [3H]thymidine incorporation in cells grown in 5% serum. Tritium 70-72 PCNA clamp associated factor Homo sapiens 30-33 8207430-4 1994 The A2A agonist CGS-21680 (30 nM) produced a greater increase of the evoked release of [3H]ACh in the CA3 than in the dentate gyrus areas, whereas no consistent effect was found in the CA1 area or in the whole hippocampal slice. Tritium 88-90 carbonic anhydrase 3 Rattus norvegicus 102-105 8930795-10 1996 In addition to the higher tissue uptake observed in M to F than F to M transport direction as measured by the drop in the concentration of both 3H-OX and 14C-IN in the circuits in which both compounds were added, the same trend was found for the transfer rates of both compounds. Tritium 144-146 oxytocin/neurophysin I prepropeptide Homo sapiens 147-149 8207430-6 1994 Both adenosine deaminase (2 U/ml) and DPCPX (250 nM) increased the evoked release of [3H]ACh in the CA1 and CA3 areas but not in the dentate gyrus. Tritium 86-88 adenosine deaminase Rattus norvegicus 5-24 9218690-3 1997 [3H]8-OH-DPAT appears to bind to a single population of binding sites in both the hippocampus and the dorsal raphe nucleus, although the K(d) for the radioligand at the dorsal raphe site was five times that observed at the hippocampal 5-HT1A receptor. Tritium 1-3 5-hydroxytryptamine receptor 1A Homo sapiens 235-250 8207430-6 1994 Both adenosine deaminase (2 U/ml) and DPCPX (250 nM) increased the evoked release of [3H]ACh in the CA1 and CA3 areas but not in the dentate gyrus. Tritium 86-88 carbonic anhydrase 3 Rattus norvegicus 108-111 7524066-8 1994 GRP (10(-10) to 10(-6) M) produced dose-dependent increments in [3H]acetylcholine release from pancreatic lobules. Tritium 65-67 gastrin releasing peptide Rattus norvegicus 0-3 8011671-4 1994 Analysis of radiolabeled transferrin receptor protein from [3H]myristate-labeled cells by acid hydrolysis followed by thin layer chromatography revealed the exclusive presence of [3H]myristate. Tritium 60-62 transferrin Mus musculus 25-36 8011671-5 1994 Labeled transferrin receptor protein from [3H]palmitate-labeled cells contained predominantly [3H]stearate and smaller amounts of [3H]palmitate. Tritium 43-45 transferrin Mus musculus 8-19 8011671-5 1994 Labeled transferrin receptor protein from [3H]palmitate-labeled cells contained predominantly [3H]stearate and smaller amounts of [3H]palmitate. Tritium 43-46 transferrin Mus musculus 8-19 8877882-4 1996 The objective of this study was to examine the inhibitory effects of various organic compounds, most of which have been studied previously in MDR cancer cells, on P-gp-mediated [3H]DNM uptake into cLPM. Tritium 178-180 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 163-167 8877882-10 1996 Phosphatidylcholine, a substrate for the gene product of the class III P-gp gene, produced significant inhibition of [3H]DNM transport (30.6% at a 10-fold-higher substrate concentration), suggesting that transport may be mediated, at least in part, by this P-gp gene product. Tritium 118-120 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 71-75 8877882-10 1996 Phosphatidylcholine, a substrate for the gene product of the class III P-gp gene, produced significant inhibition of [3H]DNM transport (30.6% at a 10-fold-higher substrate concentration), suggesting that transport may be mediated, at least in part, by this P-gp gene product. Tritium 118-120 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 257-261 8876034-1 1996 The novel radioligand [3H]PD140376 was used to label receptors that bind cholecystokinin (CCK) and related peptides in membranes prepared from guinea-pig brain and gastric glands. Tritium 23-25 cholecystokinin Cavia porcellus 73-88 8876034-1 1996 The novel radioligand [3H]PD140376 was used to label receptors that bind cholecystokinin (CCK) and related peptides in membranes prepared from guinea-pig brain and gastric glands. Tritium 23-25 cholecystokinin Cavia porcellus 90-93 8842447-5 1996 Infection of CHO-K1 cells with the rat P2X2 purinoceptor using Semliki forest virus (SFV) resulted in the expression of high affinity (pKd = 9.3; Bmax = 18.1 pmol mg-1 protein) binding sites for [35S]-ATP gamma S but not for [3H]-alpha, beta-methylene ATP ([3H]-alpha beta meATP). Tritium 226-228 purinergic receptor P2X 2 Rattus norvegicus 39-43 8842447-5 1996 Infection of CHO-K1 cells with the rat P2X2 purinoceptor using Semliki forest virus (SFV) resulted in the expression of high affinity (pKd = 9.3; Bmax = 18.1 pmol mg-1 protein) binding sites for [35S]-ATP gamma S but not for [3H]-alpha, beta-methylene ATP ([3H]-alpha beta meATP). Tritium 258-260 purinergic receptor P2X 2 Rattus norvegicus 39-43 7970171-4 1994 We report that 24 h after a single dose, PCP increases N-methyl-D-aspartate (NMDA)-sensitive [3H]glutamate binding in hippocampus (CA1) in an apparent dose-sensitive manner; no other dose-sensitive regional changes in NMDA binding sites were apparent in a sampling of 19 brain regions. Tritium 94-96 carbonic anhydrase 1 Homo sapiens 131-134 9218690-4 1997 Similarly, although 5-HT and selective 5-HT1A receptor ligands displayed high affinity for the [3H]8-OH-DPAT binding site in the dorsal raphe nucleus, the affinity at the dorsal raphe site was less than that observed at the hippocampal 5-HT1A receptor. Tritium 96-98 5-hydroxytryptamine receptor 1A Homo sapiens 39-54 9166661-5 1997 All the cell lines expressing mutant IRS-1 showed a significant reduction in [3H]thymidine incorporation compared with WT. Tritium 78-80 insulin receptor substrate 1 Homo sapiens 37-42 8002947-4 1994 We studied the subcellular distribution of the liver InsP3R by measuring the maximal binding capacity of [3H]InsP3 and using antibodies against the 14 C-terminal residues of the type 1 InsP3R. Tritium 106-108 inositol 1,4,5-trisphosphate receptor, type 1 Rattus norvegicus 53-59 8869766-3 1996 This distribution of [3H]sumatriptan-binding sites in the human brain shows some differences when compared with that of 5HT1D receptors, confirming that, besides 5HT1D, sumatriptan also binds to 5HT1F receptor subtype. Tritium 22-24 5-hydroxytryptamine receptor 1D Homo sapiens 162-167 9190843-6 1997 The cholinesterase inhibitors displaced [3H]oxo-M in human brain at 15- to 250-fold higher concentrations, that is with pKi values of 6.3, 4.6 and 4.2, respectively. Tritium 41-43 butyrylcholinesterase Homo sapiens 4-18 8764607-3 1996 However, many of these treatments had a marginal effect on [3H]dAdo transport. Tritium 60-62 ado Drosophila melanogaster 63-67 8764607-6 1996 Uptake of (3H]norepinephrine ([3H]NE) decreased in neurons damaged by nucleosides and increased to control levels when neurons were protected by various agents against adenosine or dAdo toxicity. Tritium 11-13 ado Drosophila melanogaster 181-185 8002947-5 1994 The antibodies recognized a large amount of an InsP3R protein of 260 kDa in a membrane fraction which is also enriched with [3H]InsP3-binding sites and with markers of the basal, the lateral and the bile-canalicular membrane and the plasma-membrane Ca2+ pump (PMCA). Tritium 125-127 inositol 1,4,5-trisphosphate receptor, type 1 Rattus norvegicus 47-53 8204587-4 1994 Reduction of pentalenolactone with tritium gas gave [2,3,7,8-3H4]tetrahydropentalenolactone (7), which also exhibited time-dependent, irreversible inactivation of GAPDH. Tritium 35-42 glyceraldehyde-3-phosphate dehydrogenase Oryctolagus cuniculus 163-168 9190858-0 1997 CP-96,345, which inhibits [3H] substance P binding, selectively inhibits the behavioral response to intrathecally administered N-methyl-D-aspartate, but not substance P, in the mouse. Tritium 27-29 tachykinin 1 Mus musculus 31-42 8203532-4 1994 Exposure to stress led to appearance of c-fos mRNA and oncoprotein in the gastric oxyntic gland mucosa at 2 h and its disappearance by 4 h. Baseline expression of c-myc was enhanced significantly after 6 h of stress and remained elevated for 4 h. This change in the expression of c-fos and c-myc mRNA and oncoprotein preceded an increased rate of [3H]thymidine incorporation into mucosal DNA. Tritium 348-350 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 40-45 8918981-2 1996 We report here the effects of androgenic/anabolic steroids (AAS) on the binding of the PBR-specific ligand [3H] PK11195 to male rat brain cortical synaptoneurosomes. Tritium 108-110 translocator protein Rattus norvegicus 87-90 9190858-7 1997 The more potent inhibitor of [3H] SP binding, (+)-(2S,3S)-3-(2-methoxybenzylamino)-2-phenylpiperidine (CP-99,994), was approximately 10 times more potent in inhibiting NMDA-induced activity than CP-96,345. Tritium 30-32 tachykinin 1 Mus musculus 34-36 7908295-8 1994 In rat chondrocytes exposed to CNP, [3H]thymidine incorporation was inhibited in a dose-dependent manner (half-maximal response, 10(-11)M). Tritium 37-39 natriuretic peptide C Rattus norvegicus 31-34 9178948-4 1997 In control animals, [3H]DHA and [3H]PAM were incorporated into the synaptosomal fractions, representing 50%-60% of total membrane label. Tritium 33-35 peptidylglycine alpha-amidating monooxygenase Rattus norvegicus 36-39 8166641-8 1994 Together, these results suggest strongly that (1) AngII phosphorylates and activates cPLA2 in a PKC-dependent manner, and that (2) cPLA2 mediates the AngII-induced [3H]arachidonic acid release in vascular smooth-muscle cells. Tritium 165-167 phospholipase A2 group IVA Homo sapiens 85-90 8166641-8 1994 Together, these results suggest strongly that (1) AngII phosphorylates and activates cPLA2 in a PKC-dependent manner, and that (2) cPLA2 mediates the AngII-induced [3H]arachidonic acid release in vascular smooth-muscle cells. Tritium 165-167 phospholipase A2 group IVA Homo sapiens 131-136 8149483-5 1994 In a more detailed time course, application of 20 nmol of TPA to mouse skin led to 20, 36, 92 and 0% decrease in the binding of [3H]RA to mouse epidermal RAR at 2, 4, 12 and 72 h after treatment respectively. Tritium 129-131 retinoic acid receptor, alpha Mus musculus 154-157 8183241-14 1994 The pharmacological profile and regional distribution suggest that the high and low affinity quisqualate-sensitive components of [3H]glutamate binding sites might correspond to the mGluR1/mGluR5 and mGluR2/mGluR3 subgroups of cloned mGluRs, respectively. Tritium 130-132 glutamate receptor, ionotropic, AMPA3 (alpha 3) Mus musculus 206-212 7510956-4 1994 The nuclear accumulation of [3H]-vitamin D3/receptor complexes (VDR) in a nuclear translocation assay was significantly enhanced (by 3 h) in the presence of camptothecin. Tritium 29-31 vitamin D receptor Homo sapiens 33-52 7510956-4 1994 The nuclear accumulation of [3H]-vitamin D3/receptor complexes (VDR) in a nuclear translocation assay was significantly enhanced (by 3 h) in the presence of camptothecin. Tritium 29-31 vitamin D receptor Homo sapiens 64-67 7510961-1 1994 Monocyte Chemotactic Protein(MCP)-1 and other members of the C-C branch of the chemokine superfamily (RANTES, MIP1 alpha/LD78, and MCP-3) induced, at chemotactic concentrations, the release of [3H]arachidonic acid in prelabeled human monocytes. Tritium 194-196 C-C motif chemokine ligand 5 Homo sapiens 102-108 8675248-4 1996 Ang-(1-7) inhibited [3H]thymidine incorporation in response to stimulation by fetal bovine serum, platelet-derived growth factor, or Ang II. Tritium 21-23 angiogenin Rattus norvegicus 0-3 8698628-4 1996 Two VP16/VM26-resistant KB cell lines, KB/VM-1 and KB/ VM-4, which were selected by stepwise exposure to VM26 had decreased accumulation of [3H]VP16 and increased levels of MRP, but no apparent expression of MDR1 gene was observed. Tritium 141-143 host cell factor C1 Homo sapiens 4-8 8698628-5 1996 Another VP16/VM26-resistant KB cell line, KB/VP-4, which was further isolated from a VP16-resistant KB cell line, KB/VP-2, had decreased accumulation of [3H]VP16 and showed overexpression of MRP gene, but not that of MDR1 gene. Tritium 154-156 host cell factor C1 Homo sapiens 8-12 8698628-5 1996 Another VP16/VM26-resistant KB cell line, KB/VP-4, which was further isolated from a VP16-resistant KB cell line, KB/VP-2, had decreased accumulation of [3H]VP16 and showed overexpression of MRP gene, but not that of MDR1 gene. Tritium 154-156 host cell factor C1 Homo sapiens 85-89 8698628-5 1996 Another VP16/VM26-resistant KB cell line, KB/VP-4, which was further isolated from a VP16-resistant KB cell line, KB/VP-2, had decreased accumulation of [3H]VP16 and showed overexpression of MRP gene, but not that of MDR1 gene. Tritium 154-156 host cell factor C1 Homo sapiens 85-89 8641177-4 1996 On the other hand, ammonium chloride and chloroquine, weak bases that neutralize the pH of acidic cell compartments, blocked IGFBP-3 potentiation of IGF-I-stimulated [3H]AIB uptake. Tritium 167-169 IGFI Bos taurus 149-154 8641177-6 1996 Bafilomycin A, a specific inhibitor of ATP-dependent hydrogen ion pumps, also inhibited IGFBP-3 potentiation of IGF-I-stimulated [3H]AIB uptake. Tritium 130-132 IGFI Bos taurus 112-117 8967510-7 1996 Solubilization of lung elastin with PPE produced a residue that exhibited inhibitory capacity toward HLE when either 3H-labeled aorta elastin or succinyl trialanine nitroanilide was used as a substrate. Tritium 117-119 elastin Homo sapiens 23-30 7510961-1 1994 Monocyte Chemotactic Protein(MCP)-1 and other members of the C-C branch of the chemokine superfamily (RANTES, MIP1 alpha/LD78, and MCP-3) induced, at chemotactic concentrations, the release of [3H]arachidonic acid in prelabeled human monocytes. Tritium 194-196 C-C motif chemokine ligand 7 Homo sapiens 131-136 7984276-5 1994 Using [3H]5-HT, we found that the affinities of all the mutant receptors for propranolol and pindolol were significantly increased by 100-1000 fold, 5-HT1D alpha and 5-HT1F receptors showing the highest and the 5-HT1E receptor displaying the lowest affinity. Tritium 7-9 5-hydroxytryptamine receptor 1D Homo sapiens 149-161 8967510-7 1996 Solubilization of lung elastin with PPE produced a residue that exhibited inhibitory capacity toward HLE when either 3H-labeled aorta elastin or succinyl trialanine nitroanilide was used as a substrate. Tritium 117-119 elastin Homo sapiens 134-141 7984276-5 1994 Using [3H]5-HT, we found that the affinities of all the mutant receptors for propranolol and pindolol were significantly increased by 100-1000 fold, 5-HT1D alpha and 5-HT1F receptors showing the highest and the 5-HT1E receptor displaying the lowest affinity. Tritium 7-9 5-hydroxytryptamine receptor 1F Homo sapiens 166-172 9200494-4 1997 The selective neurokinin NK1 receptor agonist [Sar9, met(O2)11]SP also potentiated K(+)-evoked release of [3H]ACh. Tritium 107-109 tachykinin receptor 1 Rattus norvegicus 25-37 8199879-0 1994 Quantitative autoradiography of 5-HT1D and 5-HT1E binding sites labelled by [3H]5-HT, in frontal cortex and the hippocampal region of the human brain. Tritium 77-79 5-hydroxytryptamine receptor 1D Homo sapiens 32-38 8199879-1 1994 In human cortex and hippocampus area, [3H]5-HT (5 nM) labels 5-HT1A, 5-HT1D and 5-HT1E sites. Tritium 39-41 5-hydroxytryptamine receptor 1D Homo sapiens 69-75 8199879-4 1994 5-HT1D and 5-HT1E sites, were more homogeneously distributed in layers II to VI (21-34% of specific [3H]5-HT binding). Tritium 101-103 5-hydroxytryptamine receptor 1D Homo sapiens 0-6 8796265-3 1996 This was demonstrated by [3H)ethanolamine labeling of Madin Darby canine kidney (MDCK) cells which resulted in labeling of the membrane anchor of CPM as shown by immunoprecipitation, polyacrylamide gel electrophoresis and autoradiography. Tritium 26-28 carboxypeptidase M Canis lupus familiaris 146-149 8708541-11 1996 In studies on IGFBP-6 actions, IGFBP-6 completely inhibited IGF-II-induced [3H]thymidine incorporation in MC3T3-E1 mouse osteoblast cells while it had only minimal inhibitory effects on IGF-I-induced [3H]thymidine incorporation. Tritium 76-78 insulin-like growth factor binding protein 6 Mus musculus 31-38 8708541-11 1996 In studies on IGFBP-6 actions, IGFBP-6 completely inhibited IGF-II-induced [3H]thymidine incorporation in MC3T3-E1 mouse osteoblast cells while it had only minimal inhibitory effects on IGF-I-induced [3H]thymidine incorporation. Tritium 201-203 insulin-like growth factor binding protein 6 Mus musculus 31-38 9176200-2 1997 Gln was additive to epidermal growth factor (EGF) and insulin-like growth factor I (IGF-I) in stimulating DNA synthesis, as assessed by [3H]thymidine incorporation. Tritium 137-139 insulin-like growth factor 1 Rattus norvegicus 54-82 8615681-3 1996 Thereafter [3H]lyso-PAF was translocated into cells with concomitant reacylation to [3H]alkyl-acyl-glycerophosphocholine. Tritium 12-14 PCNA clamp associated factor Rattus norvegicus 20-23 8129709-5 1994 Incubation of endothelial cells with vitamin E resulted in an increase in the formation of [3H]alkylacyl-GPC from [3H]alkyl-GPC. Tritium 92-94 glycophorin C (Gerbich blood group) Homo sapiens 105-108 8129709-5 1994 Incubation of endothelial cells with vitamin E resulted in an increase in the formation of [3H]alkylacyl-GPC from [3H]alkyl-GPC. Tritium 92-94 glycophorin C (Gerbich blood group) Homo sapiens 124-127 8129709-5 1994 Incubation of endothelial cells with vitamin E resulted in an increase in the formation of [3H]alkylacyl-GPC from [3H]alkyl-GPC. Tritium 115-117 glycophorin C (Gerbich blood group) Homo sapiens 105-108 8129709-5 1994 Incubation of endothelial cells with vitamin E resulted in an increase in the formation of [3H]alkylacyl-GPC from [3H]alkyl-GPC. Tritium 115-117 glycophorin C (Gerbich blood group) Homo sapiens 124-127 7905785-8 1994 Furthermore, SST and OCT inhibited VIP-induced [3H]thymidine incorporation, cyclic AMP formation, and tyrosine kinase activity with IC50 values < 10 nM. Tritium 48-50 somatostatin Homo sapiens 13-16 8200109-2 1994 The data indicate that measured elastolytic activity in this assay system was dependent on the amount of 3H-elastin/culture well. Tritium 105-107 elastin Homo sapiens 108-115 8200109-3 1994 3H-elastin > 350 micrograms/well, in contrast to the < or = 200 micrograms/well commonly used in this assay system, resulted in optimal measurement of elastolytic activity that was linear with respect to culture time (up to 72 h examined) and was directly proportional to number of AM/well (up to 1.0 x 10(6) examined). Tritium 0-2 elastin Homo sapiens 3-10 8615681-4 1996 A portion of untransformed [3H]PAF accumulated in the outer leaflet, and only a small amount of the [3H]PAF was translocated into the inner leaflet of the plasma membrane. Tritium 28-30 PCNA clamp associated factor Rattus norvegicus 31-34 8615681-4 1996 A portion of untransformed [3H]PAF accumulated in the outer leaflet, and only a small amount of the [3H]PAF was translocated into the inner leaflet of the plasma membrane. Tritium 101-103 PCNA clamp associated factor Rattus norvegicus 104-107 8603600-10 1996 Coadministration of 30 micrograms IGF-2 blocked the neuroprotective effects of 20 micrograms IGF-1 (n = 18, P < 0.05) and reduced the accumulation of [3H]IGF-1 in the injured hemisphere (n = 4) (P < 0.05). Tritium 154-156 insulin-like growth factor 2 Rattus norvegicus 34-39 8600167-7 1996 In contrast, the mitogen activated protein kinase (enzyme which has been shown to phosphorylate pRb) was constitutively active in BP-A31 cells, and its activity was not significantly affected by a < or = 3h incubation with sodium butyrate. Tritium 207-209 RB transcriptional corepressor 1 Mus musculus 96-99 9176200-2 1997 Gln was additive to epidermal growth factor (EGF) and insulin-like growth factor I (IGF-I) in stimulating DNA synthesis, as assessed by [3H]thymidine incorporation. Tritium 137-139 insulin-like growth factor 1 Rattus norvegicus 84-89 8636428-1 1996 Treatment of quiescent rat aortic smooth muscle cells with either alpha-thrombin or a thrombin receptor-derived agonist peptide (SFLLRNP) resulted in pronounced increases in [3H]thymidine incorporation that were concentration dependent and reached a maximum of approximately 15-fold above serum-starved controls. Tritium 175-177 coagulation factor II (thrombin) receptor Rattus norvegicus 86-103 8286381-3 1994 [3H]Ryanodine-binding assays indicated 10.6 +/- 0.9 pmol of high-affinity ryanodine binding per milligram of SR protein. Tritium 1-3 serine/arginine-rich-splicing factor SR34 Triticum aestivum 109-119 8866701-7 1996 [3H]PK 11195 was used to label platelet PBR. Tritium 1-3 translocator protein Homo sapiens 40-43 8294488-2 1994 The [3H]azido-Q-labeled cytochrome b protein was purified to homogeneity from the azido-Q-labeled ubiquinol-cytochrome c reductase by a procedure involving Triton X-100 and urea treatment, calcium phosphate column chromatography, acetone precipitation, decanoyl-N-methylglucamide-cholate extraction, and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Tritium 5-7 cytochrome b Bos taurus 24-36 9151782-8 1997 A synthetic pentadecapeptide, corresponding to a region unique to INGAP, stimulated a 2.4-fold increase in [3H]thymidine incorporation into hamster duct epithelium in primary culture and a rat pancreatic duct cell line but had no effect on a hamster insulinoma tumor cell line. Tritium 108-110 regenerating family member 3 alpha Homo sapiens 66-71 7506726-7 1994 Activation by anti-CD7 mAb cross-linked to plastic or through goat anti-mouse Ig also induced a variety of NK cell functions: it stimulated secretion of IFN-gamma, led to proliferation of NK cells, as measured by [3H]thymidine incorporation, and significantly enhanced cytotoxicity of NK cells against K562 targets (p < 0.03). Tritium 214-216 CD7 molecule Homo sapiens 19-22 8852830-7 1996 The rank order for displacement of the specific binding of the GR ligand [3H]dexamethasone from the canine liver receptor was: dexamethasone > cortisol > MPA > PROL > progesterone >> aldosterone approximately spironolactone. Tritium 74-76 nuclear receptor subfamily 3 group C member 1 Canis lupus familiaris 63-65 9111052-2 1997 To delineate ligand binding and functional characteristics of the human B1 kinin receptor, a stable clone of Chinese hamster ovary cells expressing a single class of binding sites for [3H]des-Arg10-lysylbradykinin with a Kd of 0.3 nM and a Bmax of 38 fmol/mg protein ( approximately 40,000 receptors/cell) was isolated. Tritium 185-187 bradykinin receptor B2 Homo sapiens 81-89 8304465-3 1994 Transforming growth factor-alpha (TGF-alpha) and epidermal growth factor (EGF) increased [3H]thymidine incorporation, cell number, and labeling index above control. Tritium 90-92 TGF alpha Oryctolagus cuniculus 0-32 9111052-4 1997 In marked contrast to cloned Chinese hamster ovary cells expressing the human kinin B2 receptor, which internalized approximately 80% of the ligand within 5 min upon exposure to 2 nM [3H]bradykinin, exposure of cells expressing the B1 receptor to 1 nM [3H]des-Arg10-lysylbradykinin resulted in minimal ligand internalization. Tritium 184-186 bradykinin receptor B2 Homo sapiens 87-95 8304465-3 1994 Transforming growth factor-alpha (TGF-alpha) and epidermal growth factor (EGF) increased [3H]thymidine incorporation, cell number, and labeling index above control. Tritium 90-92 protransforming growth factor alpha Oryctolagus cuniculus 34-43 9111052-4 1997 In marked contrast to cloned Chinese hamster ovary cells expressing the human kinin B2 receptor, which internalized approximately 80% of the ligand within 5 min upon exposure to 2 nM [3H]bradykinin, exposure of cells expressing the B1 receptor to 1 nM [3H]des-Arg10-lysylbradykinin resulted in minimal ligand internalization. Tritium 253-255 bradykinin receptor B2 Homo sapiens 87-95 9083274-8 1997 Reducing the levels of p27 resulted in an increased magnitude of mesangial cell proliferation (BrdU and 3H-thymidine incorporation) induced by PDGF and bFGF compared to non-transfected mesangial cells and mesangial cells transfected with control mismatch oligodeoxynucleotides. Tritium 104-106 cyclin-dependent kinase inhibitor 1B Rattus norvegicus 23-26 8124585-3 1994 Normal animals had high [3H]IP3 binding in the CA1 subfield of the hippocampus and the striatum. Tritium 25-27 carbonic anhydrase 1 Homo sapiens 47-50 9096697-2 1997 Pretreatment with GM-CSF (100 U/ml) for 48 h resulted in a significant enhancement of DNA synthesis in both cell types: 21 of 35 AML specimens were found to be responsive to GM-CSF as defined by an increase of 3H-TdR incorporation into the DNA > 1.5-fold while NBMMC from normal donors were responsive in all cases. Tritium 210-212 colony stimulating factor 2 Homo sapiens 18-24 8040836-2 1994 Both granulocyte-macrophage colony stimulating factor (GM-CSF) and colony stimulating factor-1 (CSF-1) increased [3H]thymidine (TdR) uptake (3-8 times control values) by trophoblast harvested from placentae on day 12 or 14 of pregnancy. Tritium 114-116 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 5-53 8040836-2 1994 Both granulocyte-macrophage colony stimulating factor (GM-CSF) and colony stimulating factor-1 (CSF-1) increased [3H]thymidine (TdR) uptake (3-8 times control values) by trophoblast harvested from placentae on day 12 or 14 of pregnancy. Tritium 114-116 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 55-61 8040836-2 1994 Both granulocyte-macrophage colony stimulating factor (GM-CSF) and colony stimulating factor-1 (CSF-1) increased [3H]thymidine (TdR) uptake (3-8 times control values) by trophoblast harvested from placentae on day 12 or 14 of pregnancy. Tritium 114-116 colony stimulating factor 1 (macrophage) Mus musculus 67-94 8040836-2 1994 Both granulocyte-macrophage colony stimulating factor (GM-CSF) and colony stimulating factor-1 (CSF-1) increased [3H]thymidine (TdR) uptake (3-8 times control values) by trophoblast harvested from placentae on day 12 or 14 of pregnancy. Tritium 114-116 colony stimulating factor 1 (macrophage) Mus musculus 96-101 8302285-6 1994 [3H]NKA bound to hamster NK-2R receptor in a protein-dependent, high affinity (Kd1 = 4.14 +/- 0.31 nM), saturable (Bmax1 = 679 +/- 26 fmol/mg of protein), and highly specific manner (89 +/- 2%). Tritium 1-3 tachykinin receptor 2 Homo sapiens 25-30 21043740-5 1994 Thrombin caused rapid accumulation of [(3)H]-InsP(3) or of inositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)]. Tritium 38-43 prothrombin Oryctolagus cuniculus 0-8 9096697-2 1997 Pretreatment with GM-CSF (100 U/ml) for 48 h resulted in a significant enhancement of DNA synthesis in both cell types: 21 of 35 AML specimens were found to be responsive to GM-CSF as defined by an increase of 3H-TdR incorporation into the DNA > 1.5-fold while NBMMC from normal donors were responsive in all cases. Tritium 210-212 colony stimulating factor 2 Homo sapiens 174-180 8045200-2 1994 Binding to PBR was studied using [3H]PK11195 as radioligand. Tritium 34-36 translocator protein Rattus norvegicus 11-14 9089283-4 1997 Also, the synthesis of phospholipids from tritium-labeled fatty acid as precursor was left unchanged by the p56(lck) inhibitor. Tritium 42-49 LCK proto-oncogene, Src family tyrosine kinase Homo sapiens 108-116 8280078-15 1993 When hepatocytes were incubated with [2-3H, U-14C]glucose, the incorporation of 3H/14C label into glycogen correlated with the extent of glucokinase release. Tritium 40-42 glucokinase Homo sapiens 137-148 9048591-10 1997 In contrast, platelet-derived growth factor- and serum (10%)-induced increases in [3H]thymidine incorporation remained intact. Tritium 83-85 myotrophin Rattus norvegicus 30-43 9038193-6 1997 In contrast, MAP kinase activation is blocked by PD 098059, an inhibitor of MAP kinase kinase 1 (MEK1) activation, which also blunts the A2A-adenosine receptor-mediated increase in [3H]thymidine incorporation. Tritium 182-184 mitogen-activated protein kinase kinase 1 Homo sapiens 76-95 8136414-12 1993 The rate of incorporation of [3H]thymidine in murine splenocytes was dose-dependently enhanced only by bound prolactin fraction. Tritium 30-32 prolactin Mus musculus 109-118 9038193-6 1997 In contrast, MAP kinase activation is blocked by PD 098059, an inhibitor of MAP kinase kinase 1 (MEK1) activation, which also blunts the A2A-adenosine receptor-mediated increase in [3H]thymidine incorporation. Tritium 182-184 mitogen-activated protein kinase kinase 1 Homo sapiens 97-101 8228253-4 1993 Additional studies demonstrate that MLD, SLD, and other less potent PLA2 inhibitors such as 4-bromophenacylbromide and nordihydroguiaretic acid inhibit the surface expression of MAC-1 (IC50: MLD, 0.33 microM; SLD, 0.23 microM; 4-bromophenacylbromide, 2.8 microM; NDGA, 3.5 microM) at concentrations similar to those at which they inhibit [3H]AA release. Tritium 339-341 integrin subunit alpha M Homo sapiens 178-183 9017662-4 1997 The pharmacological profile of the [3H]8-OH-DPAT binding sites was consistent with the labelling of the 5-HT1A receptor in the hippocampus and cortex, whereas the striatal site shared strong similarity to the presynaptic serotonin transporter. Tritium 36-38 5-hydroxytryptamine receptor 1A Homo sapiens 104-119 9203641-6 1997 The truncated EP4 receptor was stably expressed in Chinese hamster ovary cells at levels comparable to that of the wild-type receptor and exhibited a Kd value for [3H]PGE2 binding similar to that of the wild-type receptor. Tritium 164-166 prostaglandin E receptor 4 Homo sapiens 14-17 8297002-4 1993 If required, the mass amounts of cyclic AMP and cyclic GMP present could be determined by measurement of the specific radioactivities of the precursor [3H]ATP and [3H]GTP, respectively. Tritium 152-154 5'-nucleotidase, cytosolic II Homo sapiens 55-58 8297002-4 1993 If required, the mass amounts of cyclic AMP and cyclic GMP present could be determined by measurement of the specific radioactivities of the precursor [3H]ATP and [3H]GTP, respectively. Tritium 164-166 5'-nucleotidase, cytosolic II Homo sapiens 55-58 8121637-2 1993 Displacement of the [3H]pargyline binding on MAO-A (L(-)-deprenyl suppressed binding to MAO-B) by harman, 1-methyl-4-phenylpyridinium (MPP+) and 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP) revealed IC50 values of 250 +/- 100 nM, 3.1 +/- 0.8 microM, and 5.1 +/- 0.4 microM, respectively. Tritium 21-23 monoamine oxidase B Rattus norvegicus 88-93 9037397-0 1997 Autoradiographic localization of 5-HT1A receptors in the post-mortem human brain using [3H]WAY-100635 and [11C]way-100635. Tritium 88-90 5-hydroxytryptamine receptor 1A Homo sapiens 33-39 8115030-2 1993 Cholecystokinin octapeptide (CCK8) (10(-10)-10(-7) M) enhanced the release of [3H]ACh and the contractions of the muscle. Tritium 79-81 cholecystokinin Cavia porcellus 0-15 9037397-5 1997 The labeling of human 5-HT1A receptors with [3H]WAY-100635 was antagonised by the addition of 5-HT1A receptor ligands, 5-HT, buspirone, pindolol or 8-OH-DPAT (10 microM), leaving a very low background of non-specific binding. Tritium 45-47 5-hydroxytryptamine receptor 1A Homo sapiens 22-28 9037397-5 1997 The labeling of human 5-HT1A receptors with [3H]WAY-100635 was antagonised by the addition of 5-HT1A receptor ligands, 5-HT, buspirone, pindolol or 8-OH-DPAT (10 microM), leaving a very low background of non-specific binding. Tritium 45-47 5-hydroxytryptamine receptor 1A Homo sapiens 22-37 8263365-3 1993 It appears that the BK-induced [3H]arachidonic acid release was attributed to the activation of phospholipase A2, since a phospholipase A2 inhibitor, mepacrine, significantly inhibited the BK enhancement of [3H]arachidonic acid release whereas a diacylglycerol lipase inhibitor, RHC80267, failed to do so. Tritium 32-34 phospholipase A2, group IB, pancreas Mus musculus 96-112 9037397-7 1997 The selective labeling of 5-HT1A receptors with [3H]WAY-100635 clearly show that this compound is useful for further studies of the human 5-HT1a receptor subtype in vitro [11C]WAY-100635 is used for the characterization of 5-HT1A receptors with positron emission tomography (PET). Tritium 49-51 5-hydroxytryptamine receptor 1A Homo sapiens 26-32 7507605-15 1993 Although both insulin and EGF increased [3H]thymidine incorporation in heart cells from 8-day embryos, different responses to insulin and EGF occurred with heart cells from 18-day embryos. Tritium 41-43 epidermal growth factor Gallus gallus 26-29 7507605-16 1993 Whereas the heart cells from 18-day embryos still responded to EGF by increasing [3H]thymidine incorporation, they did not show a response to insulin as measured by [3H]thymidine incorporation, suggesting that the loss of response of the heart cells to growth signals may occur at the receptor level. Tritium 82-84 epidermal growth factor Gallus gallus 63-66 9037399-4 1997 At the same time, treatment with NR1 antisense ODN significantly reduced the Bmax of [3H]CGS-19755 binding (2101 fmol/mg protein) compared to both vehicle (2787 fmol/mg protein) and sense (2832 +/- 39 fmol/mg protein) controls without any significant change in KD (33 nM). Tritium 86-88 glutamate receptor, ionotropic, NMDA1 (zeta 1) Mus musculus 33-36 8258364-1 1993 The effect of chronic ethanol consumption (60 days) on 5-HT1C receptors as measured by [3H]mesulergine binding in the hippocampus, cortex, and choroid plexus of rats was investigated. Tritium 88-90 5-hydroxytryptamine receptor 2C Rattus norvegicus 55-61 8373414-6 1993 PCK mRNA and PCK activity were elevated after 2h and 3h, respectively, to 100% at 16% O2 (mimicking arterial oxygen tensions) and to about 60% at 8% O2 (mimicking venous oxygen tensions). Tritium 53-55 phosphoenolpyruvate carboxykinase 1 Rattus norvegicus 0-3 8373414-6 1993 PCK mRNA and PCK activity were elevated after 2h and 3h, respectively, to 100% at 16% O2 (mimicking arterial oxygen tensions) and to about 60% at 8% O2 (mimicking venous oxygen tensions). Tritium 53-55 phosphoenolpyruvate carboxykinase 1 Rattus norvegicus 13-16 8366352-1 1993 Recently, it was proposed that neuropeptide Y (NPY) and peptide YY (PYY) could act as endogenous ligands for sigma binding sites, as both NPY and PYY competed with high affinity (nanomolar) for 3H-(+)SKF 10,047 binding sites in rat brain membrane homogenates (Roman et al., 1989). Tritium 194-196 peptide YY Rattus norvegicus 56-66 8366352-1 1993 Recently, it was proposed that neuropeptide Y (NPY) and peptide YY (PYY) could act as endogenous ligands for sigma binding sites, as both NPY and PYY competed with high affinity (nanomolar) for 3H-(+)SKF 10,047 binding sites in rat brain membrane homogenates (Roman et al., 1989). Tritium 194-196 peptide YY Rattus norvegicus 68-71 8366352-1 1993 Recently, it was proposed that neuropeptide Y (NPY) and peptide YY (PYY) could act as endogenous ligands for sigma binding sites, as both NPY and PYY competed with high affinity (nanomolar) for 3H-(+)SKF 10,047 binding sites in rat brain membrane homogenates (Roman et al., 1989). Tritium 194-196 peptide YY Rattus norvegicus 146-149 9037399-5 1997 A corresponding reduction of [3H]CGP-39653 binding was also observed after treatment with NR1 antisense compared to both sense and vehicle controls. Tritium 30-32 glutamate receptor, ionotropic, NMDA1 (zeta 1) Mus musculus 90-93 8990205-12 1997 The rank order of tachykinin peptides competing for [3H]senktide binding at the NK-3 receptor homolog was [MePhe7]neurokinin B > senktide > substance P = neurokinin A > neurokinin B. Tritium 53-55 tachykinin precursor 3 Homo sapiens 114-126 8393873-8 1993 Northern blot analysis and the specific uptake of [3H]1,25-(OH)2D3 revealed unexpectedly that the vitamin D receptor was abundantly expressed in MT-2 cells. Tritium 51-53 vitamin D receptor Homo sapiens 98-116 9059547-3 1997 Additionally, we measured the effect of anti-IGF I antiserum on xanthine/xanthine oxidase- and H2O2-stimulated [3H]thymidine incorporation. Tritium 112-114 insulin-like growth factor 1 Rattus norvegicus 45-50 8244415-6 1993 Three IgG1 mAbs were identified by immunoreactivity with purified MMP-9 and are designated 6-6B, 7-11C, and 8-3H. Tritium 110-112 matrix metallopeptidase 9 Homo sapiens 66-71 8101558-3 1993 Release of NO upon the addition of muscarinic agonists to unlabeled neuroblastoma cells (NO donor cells) was quantitated indirectly by its ability to increase the [3H]cyclic GMP level in labeled cells whose muscarinic receptors were inactivated by irreversible alkylation (NO detector cells). Tritium 164-166 5'-nucleotidase, cytosolic II Homo sapiens 174-177 9405967-10 1997 The tritium incorporation was increased in the fundus of the stomach and the proximal duodenum in the EGF-treated animals after 3 days, whereas no significant increase in tritiated thymidine incorporation could be detected in the EGF-treated animals after 1 and 7 days compared to the controls. Tritium 4-11 epidermal growth factor Mus musculus 102-105 8105492-4 1993 Thereafter, [3H]MK-801 binding exhibited a significant reduction in the dorsolateral striatum, most of hippocampal CA1 sector and dentate gyrus 48 h or 7 days of recirculation. Tritium 13-15 carbonic anhydrase 1 Homo sapiens 115-118 8105492-5 1993 However, [3H]MK-801 binding progressively depressed in the hippocampal CA1 sector 1 month after ischemia, whereas other regions showed no significant alteration in the binding. Tritium 10-12 carbonic anhydrase 1 Homo sapiens 71-74 8395303-8 1993 There was a positive correlation between ChAT activity and [3H]CHA binding (r = 0.769), but not [3H]U-69593 binding (r = 0.197). Tritium 60-62 choline O-acetyltransferase Homo sapiens 41-45 8978713-5 1997 However, coaddition of K-252b (EC50 of approximately 2 nM) with 10-100 ng/ml NT-3 led to incorporation of [3H]thymidine in trkA expressing cells to a level induced by optimal concentrations of nerve growth factor (NGF). Tritium 107-109 neurotrophin 3 Rattus norvegicus 77-81 8396278-5 1993 All compounds studied displaced [3H]TCDD from the cytosolic Ah receptor at a concentration of 10(-10)-10(-9) M. Benzo[c]chrysene and to a lesser extent 6-methylchrysene were the only compounds capable of stimulating epoxide hydrolase activity, but the effect was modest. Tritium 33-35 aryl hydrocarbon receptor Rattus norvegicus 60-71 8978713-6 1997 The K-252b- and NT-3-induced [3H]thymidine incorporation correlated with an increase in the tyrosine autophosphorylation of the trkA receptor as well as tyrosine phosphorylation of trk-associated phospholipase C-gamma 1 and SH2-containing proteins. Tritium 30-32 neurotrophin 3 Rattus norvegicus 16-20 9075826-4 1997 Incubation of VSMCs with MCP-1 (50-200 ng/ml) in the presence of 0.5% FCS significantly increased cell proliferation, [3H]-thymidine incorporation and the proliferative S fraction, measured by flow cytometry, compared to control cells. Tritium 119-121 C-C motif chemokine ligand 2 Rattus norvegicus 25-30 8397875-3 1993 Chronic ethanol consumption resulted in a decrease in the maximum number of binding sites (Bmax) of [3H]-PDBU binding to membranal PKC without significant change in the apparent dissociation constant (KD) in the rat cortex. Tritium 101-103 protein kinase C, gamma Rattus norvegicus 131-134 8104885-3 1993 These CD4+ T cells were T-cell receptor (TcR)/CD3+ and the cross-linking of either TcR alone or both TcR and CD4 molecules induced [3H]thymidine deoxyribose (TdR) incorporation, similar to functional CD4+ T cells. Tritium 132-135 Cd4 molecule Rattus norvegicus 6-9 8104885-3 1993 These CD4+ T cells were T-cell receptor (TcR)/CD3+ and the cross-linking of either TcR alone or both TcR and CD4 molecules induced [3H]thymidine deoxyribose (TdR) incorporation, similar to functional CD4+ T cells. Tritium 132-135 Cd4 molecule Rattus norvegicus 109-112 8104885-3 1993 These CD4+ T cells were T-cell receptor (TcR)/CD3+ and the cross-linking of either TcR alone or both TcR and CD4 molecules induced [3H]thymidine deoxyribose (TdR) incorporation, similar to functional CD4+ T cells. Tritium 132-135 Cd4 molecule Rattus norvegicus 109-112 8104885-4 1993 Stimulation with a T-cell mitogen, concanavalin A (Con A), also induced the [3H]TdR incorporation and interleukin-2 receptor (IL-2R) expression in LEC rat CD4+ T cells. Tritium 77-79 Cd4 molecule Rattus norvegicus 155-158 9437722-0 1997 Binding of [3H][D-Ala2, MePhe4, Gly-ol5] enkephalin, [3H][D-Pen2, D-Pen5]enkephalin, and [3H]U-69,593 to airway and pulmonary tissues of normal and sensitized rats. Tritium 12-14 proenkephalin Rattus norvegicus 41-51 8336080-1 1993 Tumor necrosis factor alpha (TNF-alpha) more than doubles tritiated thymidine ([3H]TdR) uptake in mouse macrophages stimulated by macrophage colony-stimulating factor (CSF-1). Tritium 80-82 colony stimulating factor 1 (macrophage) Mus musculus 168-173 8336080-4 1993 We show that the TNF-alpha-induced increase in [3H]TdR uptake of CSF-1-stimulated macrophages is directly proportional to an increase in the DNA content of the culture and that the effects of TNF-alpha are direct and independent of cell number. Tritium 48-50 colony stimulating factor 1 (macrophage) Mus musculus 65-70 8336080-6 1993 Although TNF-alpha alone does not stimulate macrophage proliferation, its presence in S1 cell cultures coming to quiescence after withdrawal of CSF-1 greatly increases subsequent CSF-1-stimulated [3H]TdR uptake as the cells reenter the cycle. Tritium 197-199 colony stimulating factor 1 (macrophage) Mus musculus 144-149 8336080-6 1993 Although TNF-alpha alone does not stimulate macrophage proliferation, its presence in S1 cell cultures coming to quiescence after withdrawal of CSF-1 greatly increases subsequent CSF-1-stimulated [3H]TdR uptake as the cells reenter the cycle. Tritium 197-199 colony stimulating factor 1 (macrophage) Mus musculus 179-184 8391435-7 1993 In addition, the activity of acetyl-CoA:1-alkyl-sn-glycero-3-phosphorylcholine 2-O-acetyltransferase was transiently increased in porin-stimulated HUVEC and, after incubation with [3H]CoASAc or [3H]acetate, the [3H]acetyl group was incorporated into newly synthesized PAF. Tritium 181-183 CAS1 domain containing 1 Homo sapiens 81-100 8391435-7 1993 In addition, the activity of acetyl-CoA:1-alkyl-sn-glycero-3-phosphorylcholine 2-O-acetyltransferase was transiently increased in porin-stimulated HUVEC and, after incubation with [3H]CoASAc or [3H]acetate, the [3H]acetyl group was incorporated into newly synthesized PAF. Tritium 195-197 CAS1 domain containing 1 Homo sapiens 81-100 8940374-5 1996 Recombinant human activin A inhibited [3H]thymidine incorporation in a dose-dependent fashion with an ED50 of approximately 0.43 +/- 0.3 nM. Tritium 39-41 inhibin subunit beta E Homo sapiens 18-25 8391435-7 1993 In addition, the activity of acetyl-CoA:1-alkyl-sn-glycero-3-phosphorylcholine 2-O-acetyltransferase was transiently increased in porin-stimulated HUVEC and, after incubation with [3H]CoASAc or [3H]acetate, the [3H]acetyl group was incorporated into newly synthesized PAF. Tritium 195-197 CAS1 domain containing 1 Homo sapiens 81-100 8101133-8 1993 Neurotensin enhanced the stimulation-evoked release of [3H]DA. Tritium 56-58 neurotensin Rattus norvegicus 0-11 8726569-3 1996 The specific binding of [3H]-ketanserin in C57BL was higher in the frontal cortex and lower in the neostriatum than in the same structures of CBA mice being indicative of the differences in 5-HT2A receptor density. Tritium 25-27 5-hydroxytryptamine (serotonin) receptor 2A Mus musculus 190-205 8632339-3 1996 Furthermore, preincubation with NEM also reduced the efficacy with which the 5-HT1B/1D antagonist GR 127935 enhanced evoked overflow of [3H]5-HT. Tritium 137-139 5-hydroxytryptamine receptor 1B Rattus norvegicus 77-83 8103203-3 1993 Increased [3H]CGP39653 labeling was exclusively found in the CA3 stratum radiatum and a decreased [3H]CNQX labeling was found in several hippocampal areas. Tritium 11-13 carbonic anhydrase 3 Rattus norvegicus 61-64 8940374-6 1996 Activin (0.1-3 nM) also inhibited dihydrotestosterone (DHT)-stimulated [3H]thymidine incorporation in LNCaP cells. Tritium 72-74 inhibin subunit beta E Homo sapiens 0-7 9116208-3 1996 When NPY and muscarine were applied simultaneously, the rise in [Ca2+]i was greater than the sum of the rises of either agonist applied alone, and also evoked [3H]NA release, NPY evoked a rise in [Ca2+]i when applied during prolonged exposure to muscarine, although the peak level of [Ca2+]i was significantly lower (p < 0.05) than that reached following simultaneous application, and [3H]NA release was not stimulated. Tritium 160-162 neuropeptide Y Homo sapiens 5-8 8387366-1 1993 alpha 2 Adrenoceptors in membrane preparations of human and calf frontal cortex and of calf retina can be labelled by the antagonists [3H]idazoxan, [3H]rauwolscine and [3H]RX 821002. Tritium 135-137 adrenoceptor alpha 2A Bos taurus 0-21 8387366-1 1993 alpha 2 Adrenoceptors in membrane preparations of human and calf frontal cortex and of calf retina can be labelled by the antagonists [3H]idazoxan, [3H]rauwolscine and [3H]RX 821002. Tritium 149-151 adrenoceptor alpha 2A Bos taurus 0-21 8387366-1 1993 alpha 2 Adrenoceptors in membrane preparations of human and calf frontal cortex and of calf retina can be labelled by the antagonists [3H]idazoxan, [3H]rauwolscine and [3H]RX 821002. Tritium 149-151 adrenoceptor alpha 2A Bos taurus 0-21 8387366-2 1993 Present and previous data indicate that [3H]idazoxan possesses the highest affinity for the alpha 2 adrenoceptors in the calf tissues, whereas [3H]rauwolscine displays the highest affinity for those in the human frontal cortex. Tritium 41-43 adrenoceptor alpha 2A Bos taurus 92-113 8720594-0 1996 [3H]Sumatriptan binding sites in human brain: regional-dependent labelling of 5-HT1D and 5-HT1F receptors. Tritium 1-3 5-hydroxytryptamine receptor 1D Homo sapiens 78-84 9116208-3 1996 When NPY and muscarine were applied simultaneously, the rise in [Ca2+]i was greater than the sum of the rises of either agonist applied alone, and also evoked [3H]NA release, NPY evoked a rise in [Ca2+]i when applied during prolonged exposure to muscarine, although the peak level of [Ca2+]i was significantly lower (p < 0.05) than that reached following simultaneous application, and [3H]NA release was not stimulated. Tritium 389-391 neuropeptide Y Homo sapiens 5-8 8555249-7 1996 Increased synthesis of cPLA2 correlated well with increased [3H]arachidonic acid release, PGE2 synthesis and lipid peroxidation in epidermis after oxidant or UVB treatment. Tritium 60-64 phospholipase A2 group IVA Homo sapiens 23-28 9116208-3 1996 When NPY and muscarine were applied simultaneously, the rise in [Ca2+]i was greater than the sum of the rises of either agonist applied alone, and also evoked [3H]NA release, NPY evoked a rise in [Ca2+]i when applied during prolonged exposure to muscarine, although the peak level of [Ca2+]i was significantly lower (p < 0.05) than that reached following simultaneous application, and [3H]NA release was not stimulated. Tritium 389-391 neuropeptide Y Homo sapiens 175-178 8546715-6 1996 We show that EGF stimulates cPLA2 activity in both cell lines in vitro as measured in cytosolic fractions, but only in HER14 in vivo as measured by 3H release from cells prelabelled with [3H]arachidonic acid. Tritium 188-190 phospholipase A2 group IVA Homo sapiens 28-33 8384998-7 1993 The abundance of VDR measured by [3H]1,25(OH)2D3 binding, decreased to half without significant change in affinity, in cells differentiated by all three means compared to proliferating cells. Tritium 34-36 vitamin D receptor Homo sapiens 17-20 9116208-4 1996 Simultaneous exposure of SH-SY5Y cells to muscarine and NPY thus induces a greater than additive rise in [Ca2+]i exceeding a critical level required to evoke [3H]NA release. Tritium 159-161 neuropeptide Y Homo sapiens 56-59 9023014-1 1996 [3H]PGE2 and [3H]PGF2 alpha were shown to bind with similar binding capacity and dissociation constants to bovine aorta endothelial cells. Tritium 14-16 prostaglandin F synthase 2 Bos taurus 17-21 8490242-6 1993 Furthermore, SDS-PAGE analysis of immunoprecipitated [3H]galactose-labelled lysosomal-associated membrane protein-1 (LAMP-1) glycoprotein showed no difference in amounts or mobility. Tritium 54-56 lysosomal-associated membrane protein 1 Mus musculus 76-115 8455041-4 1993 CTB plus NGF also caused a greater inhibition of [3H]thymidine incorporation into DNA than did NGF alone. Tritium 50-52 phosphate cytidylyltransferase 1B, choline Rattus norvegicus 0-3 8510491-2 1993 Maximum stimulation of [3H] thymidine incorporation of about 2.6-fold above control was reached with 100 ng/ml IGF-1 and 500 ng/ml IGF-2, respectively. Tritium 24-26 insulin-like growth factor 2 Rattus norvegicus 131-136 8612303-5 1996 TS was suggested as the locus of action of AG337 by the ability of thymidine to antagonize cell growth inhibition and the direct demonstration of TS inhibition in whole cells using a tritium release assay. Tritium 183-190 thymidylate synthase Mus musculus 0-2 8632704-3 1996 [3H]U-69 binding sites (KD = 3.8 +/- 0.2 nM, Bmax = 6.3 +/- 0.2 fmol/mg protein) had a binding profile that correspond to kappa 1-receptor. Tritium 1-3 small nucleolar RNA, H/ACA box 69 Homo sapiens 4-8 8632713-8 1996 The results indicated that a single dose of AS-PKC-alpha at a concentration of 10-100microM caused long-lasting (for at least 4 days) inhibition (up to 55%) of 3H-thymidine incorporation by SMC. Tritium 160-162 protein kinase C, alpha Rattus norvegicus 47-56 8750737-1 1995 The role of the sigma1 receptor in the regulation of N-methyl-D-aspartate (NMDA)-stimulated [3H]dopamine release from rat striatal slices was examined. Tritium 93-95 sigma non-opioid intracellular receptor 1 Rattus norvegicus 16-31 8454606-2 1993 We have purified to homogeneity from this conditioned medium a 36-kDa protein, as measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, which inhibits [3H]thymidine incorporation into the DNA and induces cell surface expression of CD11b, the alpha chain of the adhesion receptor MAC-1, on the human promyelocytic leukemic cell line HL-60. Tritium 169-171 integrin subunit alpha M Homo sapiens 248-253 8454606-2 1993 We have purified to homogeneity from this conditioned medium a 36-kDa protein, as measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, which inhibits [3H]thymidine incorporation into the DNA and induces cell surface expression of CD11b, the alpha chain of the adhesion receptor MAC-1, on the human promyelocytic leukemic cell line HL-60. Tritium 169-171 integrin subunit alpha M Homo sapiens 296-301 8473832-5 1993 In a culture system using two interstitial cell preparations of different buoyant densities from immature rats, both EGF and transforming growth factor-alpha (TGF-alpha) caused increased [3H]thymidine incorporation in the less dense cell preparation. Tritium 188-190 transforming growth factor alpha Rattus norvegicus 125-157 8473832-5 1993 In a culture system using two interstitial cell preparations of different buoyant densities from immature rats, both EGF and transforming growth factor-alpha (TGF-alpha) caused increased [3H]thymidine incorporation in the less dense cell preparation. Tritium 188-190 transforming growth factor alpha Rattus norvegicus 159-168 8845939-9 1995 These results suggest that induction of c-fos through AMPA/kainate receptors is dependent on extracellular calcium influx and involves downstream activation of phorbol ester-sensitive protein kinase C. The effect of glutamate on oligodendrocyte progenitor proliferation was assessed by [3H]thymidine incorporation. Tritium 287-289 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 40-45 8382263-5 1993 The rank order of potency of agonists as inhibitors of the [3H]5-HT overflow was 5-HT > sumatriptan (5-HT1D/1B) > 8-hydroxy-2-(di-n-propylamino)tetralin (5-HT1A/1D) >> 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane HCl (5-HT2/1C). Tritium 60-62 5-hydroxytryptamine receptor 1D Homo sapiens 104-110 9023014-3 1996 Displacement of [3H]PGE2 performed with PGE2, PGF2 alpha or U-46619, a thromboxane agonist, shows that all three prostanoids displaced the bound [3H]PGE2 with comparable potency (IC50 = 10(-7) M). Tritium 17-19 prostaglandin F synthase 2 Bos taurus 46-50 9023014-3 1996 Displacement of [3H]PGE2 performed with PGE2, PGF2 alpha or U-46619, a thromboxane agonist, shows that all three prostanoids displaced the bound [3H]PGE2 with comparable potency (IC50 = 10(-7) M). Tritium 146-148 prostaglandin F synthase 2 Bos taurus 46-50 8613731-10 1995 The immunocytochemical data were supported by neurochemical evaluations which showed that BDNF attenuated the PCA-induced loss of 5-HT and 5-HIAA contents and 3H-5-HT uptake near the infusion cannula. Tritium 159-161 brain-derived neurotrophic factor Rattus norvegicus 90-94 8910293-5 1996 The kinetics of [3H]ACh uptake by human VAChT were saturable, exhibiting an apparent Km of 0.97 +/- 0.1 mM and Vmax of 0.58 +/- 0.04 nmol/min/mg. Tritium 17-19 solute carrier family 18 member A3 Homo sapiens 40-45 8531111-4 1995 TMB-8 is also a potent inhibitor (IC50 approximately 500 nM) of a functional, central nervous system nAChR subtype that mediates nicotinic agonist-stimulated [3H]dopamine release from rat brain synaptosomes. Tritium 159-161 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 101-106 8464354-5 1993 The myristate radiolabel associated with vimentin recovered after SDS-PAGE and subjected to acid hydrolysis was shown due to incorporated [3H]amino acids, mainly glutamic acid, generated from the fatty acid. Tritium 139-142 vimentin Bos taurus 41-49 8439537-8 1993 Uptake characteristics of [3H]retinol from CRBP and lactoglobulin were similar to that of [3H]retinol from RBP. Tritium 27-29 retinol binding protein 1 Rattus norvegicus 43-47 8383573-5 1993 The reduction of [3H]rolipram binding in the CA1 subfield of the hippocampus began 6 h after 6-min ischemia. Tritium 18-20 carbonic anhydrase 1 Homo sapiens 45-48 8615870-2 1995 Cytosolic PLA2 (cPLA2) activity could be measured in homogenates of differentiated cells, and the highly selective cPLA2 inhibitor arachidonic acid trifluoromethyl ketone reduced A23187 induced [3H]AA release from pre-labelled cells by at least 80%, with an IC50 (12.7 +/- 1.4 microM) not significantly different from that for inhibiting authentic cPLA2 (9.3 +/- 2.0 microM). Tritium 195-197 phospholipase A2 group IVA Homo sapiens 0-14 8938661-7 1996 SP, in turn, stimulates hippocampal [3H]-ACh release through NK1 receptors present on cholinergic terminals. Tritium 37-39 tachykinin receptor 1 Homo sapiens 61-64 8615870-2 1995 Cytosolic PLA2 (cPLA2) activity could be measured in homogenates of differentiated cells, and the highly selective cPLA2 inhibitor arachidonic acid trifluoromethyl ketone reduced A23187 induced [3H]AA release from pre-labelled cells by at least 80%, with an IC50 (12.7 +/- 1.4 microM) not significantly different from that for inhibiting authentic cPLA2 (9.3 +/- 2.0 microM). Tritium 195-197 phospholipase A2 group IVA Homo sapiens 115-120 8615870-2 1995 Cytosolic PLA2 (cPLA2) activity could be measured in homogenates of differentiated cells, and the highly selective cPLA2 inhibitor arachidonic acid trifluoromethyl ketone reduced A23187 induced [3H]AA release from pre-labelled cells by at least 80%, with an IC50 (12.7 +/- 1.4 microM) not significantly different from that for inhibiting authentic cPLA2 (9.3 +/- 2.0 microM). Tritium 195-197 phospholipase A2 group IVA Homo sapiens 115-120 8583698-3 1995 PAF was found to enhance a time and concentration-dependent increase in phosphotyrosine in several proteins and stimulate 3H-thymidine incorporation. Tritium 122-124 PCNA clamp associated factor Rattus norvegicus 0-3 8383573-7 1993 On the other hand, the reduction of [3H]cAMP-binding sites in the CA1 subfield began 1 day after 6-min ischemia. Tritium 37-39 carbonic anhydrase 1 Homo sapiens 66-69 8383573-8 1993 At 4 days, 47% of [3H]cAMP-binding sites in the CA1 subfield were preserved. Tritium 19-21 carbonic anhydrase 1 Homo sapiens 48-51 8440524-4 1993 As little as 0.78 micrograms/ml of BG11-G2 IgG1 anti-ricin monoclonal antibody completely protected against the in vitro toxicity of ricin as determined by [3H]leucine uptake in EL-4 cell assays. Tritium 157-159 LOC105243590 Mus musculus 43-47 8450959-8 1993 The extent of the reductions in brain-derived neurotrophic factor mRNA levels correlated with reductions in acetylcholinesterase staining density and cholinergic terminal density determined by quantitative autoradiographic analysis of [3H]vesamicol binding sites. Tritium 236-238 brain-derived neurotrophic factor Rattus norvegicus 32-65 8858921-1 1996 Treatment of cultured bovine adrenal chromaffin cells with 100 nM insulin raised [3H]saxitoxin ([3H]-STX) binding in a time-dependent manner (t1/2 = 26 h). Tritium 82-84 insulin Bos taurus 66-73 8450509-5 1993 3H-Thymidine incorporation assays as well as binding experiments, showed that these cells simultaneously respond to and bind the macrophage-specific factor CSF-1. Tritium 0-2 colony stimulating factor 1 (macrophage) Mus musculus 156-161 8446109-14 1993 TGF beta 1 inhibited TGF alpha-induced [3H]thymidine incorporation into peritubular cell DNA with cells from each developmental stage examined. Tritium 40-42 transforming growth factor alpha Rattus norvegicus 21-30 7680260-4 1993 [3H]Kainate binding was highest in the dentate gyrus followed by field CA3 and then field CA1, the ratio of the highest to the lowest being 3 to 2. Tritium 1-3 carbonic anhydrase 3 Rattus norvegicus 71-74 7680260-5 1993 Binding of [3H]DL-alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) differed, to a certain extent, from that of [3H]kainate and showed the dentate gyrus followed by field CA1 and then field CA3 in the rank order of decreasing binding. Tritium 12-14 carbonic anhydrase 3 Rattus norvegicus 200-203 8429920-6 1993 Methylation and membrane association of the small GTP-binding proteins were blocked by lovastatin, an inhibitor of 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase, which also enhanced their labeling by 3H-MVA-metabolites. Tritium 205-207 3-hydroxy-3-methylglutaryl-CoA reductase Rattus norvegicus 115-165 8748457-9 1995 [3H] Deoxyadenosine can be used as a selective agent for specific selection of deoxycytidine kinase-negative mutants. Tritium 1-3 deoxycytidine kinase Mus musculus 79-99 7677777-3 1995 C6-glial cell lines stably transfected with a human 5-HT1D alpha or 5-HT1D beta receptor gene showed a decrease (87 to 93%) in basal [3H]thymidine incorporation, whereas their MT-III mRNA content was more than 30-fold increased compared to plasmid transfected C6-glial cells. Tritium 134-136 5-hydroxytryptamine receptor 1D Homo sapiens 52-64 7574683-6 1995 Whereas only the activated MMP-2 bound to immobilized asialofetuin had significant gelatinolytic activity, both the zymogen and the activated forms of MMP-9 hydrolyzed soluble [3H]gelatin to the same extent while still bound to asialofetuin. Tritium 177-179 matrix metallopeptidase 9 Homo sapiens 151-156 7498319-4 1995 [3H]5-CT appeared to label 5-HT1B/5-HT1D sites in the substantia nigra, globus pallidus and caudate/putamen, as the binding in these regions was displaced by the 5-HT1B/1D receptor selective agents sumatriptan, CP-122,288 and GR-127,935. Tritium 1-3 5-hydroxytryptamine receptor 1B Rattus norvegicus 27-33 8858921-1 1996 Treatment of cultured bovine adrenal chromaffin cells with 100 nM insulin raised [3H]saxitoxin ([3H]-STX) binding in a time-dependent manner (t1/2 = 26 h). Tritium 97-99 insulin Bos taurus 66-73 7498319-4 1995 [3H]5-CT appeared to label 5-HT1B/5-HT1D sites in the substantia nigra, globus pallidus and caudate/putamen, as the binding in these regions was displaced by the 5-HT1B/1D receptor selective agents sumatriptan, CP-122,288 and GR-127,935. Tritium 1-3 5-hydroxytryptamine receptor 1B Rattus norvegicus 162-168 7498319-9 1995 While [3H]5-CT binding at 5-HT1B/1D sites and these atypical 5-HT1A sites was inhibited by the GTP analogue 5"-beta, gamma-imidotriphosphate, [3H]5-CT binding in the superficial cortical layers and in midline thalamic nuclei was insensitive to this agent. Tritium 7-9 5-hydroxytryptamine receptor 1B Rattus norvegicus 26-32 8383818-4 1993 [3H]Cyclic AMP binding in the CA1 subfield decreased one day after 6 min of ischemia and four days after three 2-min ischemic insults, and 62-65% of the binding was lost after 28 days. Tritium 1-3 carbonic anhydrase 1 Homo sapiens 30-33 8383818-5 1993 [3H]Rolipram binding in the CA1 subfield decreased one day after 6 min of ischemia and the binding was reduced by 45-50% after four and 28 days. Tritium 1-3 carbonic anhydrase 1 Homo sapiens 28-31 8383818-6 1993 Following three 2-min ischemic insults, [3H]rolipram binding decreased in the CA1 at one day, and decreased by 25-33% after 28 days. Tritium 41-43 carbonic anhydrase 1 Homo sapiens 78-81 7498319-13 1995 It is concluded that [3H]5-CT, in the presence of selective blockers, can be used to investigate the properties of 5-HT1A, 5-HT1B/1D and 5-HT7 receptors in the rodent brain, although further studies are required to explain the atypical features of [3H]5-CT binding in 5-HT1A receptors containing regions. Tritium 22-24 5-hydroxytryptamine receptor 1B Rattus norvegicus 123-129 8858921-2 1996 Insulin (100 nM for 4 days) increased the Bmax of [3H]STX binding by 49% without changing the KD value and also augmented the maximal influx of 22Na+ due to 560 microM veratridine by 39% without altering the EC50 value of veratridine. Tritium 51-53 insulin Bos taurus 0-7 8528563-4 1995 [3H]-U-46619 bound to A10 cells of passages 18-20 (p18-20) with two classes of sites. Tritium 1-3 cyclin-dependent kinase inhibitor 2C Rattus norvegicus 51-54 9004439-13 1996 The quantitative detection of IL-2 was performed by measuring [3H]thymidine incorporation of HT-2 cells or directly by ELISA. Tritium 63-65 interleukin 2 Mus musculus 30-34 8528563-8 1995 Cinnamophilin and SQ29548 inhibited specific [3H]-U-46619 binding to p18-20 A10 cells in a concentration-dependent manner with Ki values of 390.0 +/- 3.2 and 4.6 +/- 1.0 nM, respectively at a high affinity site, and 2.6 +/- 0.2 microM and 310.0 +/- 6.4 nM, respectively at the low affinity site. Tritium 46-48 cyclin-dependent kinase inhibitor 2C Rattus norvegicus 69-72 8528563-13 1995 U-46619 increased [3H]-thymidine incorporation into DNA of p18-20 and p28-30 A10 cells in aconcentration-dependent manner with EC50 values 362.7 +/- 27.0 and 302.5 +/- 20.1 nm, respectively. Tritium 19-21 cyclin-dependent kinase inhibitor 2C Rattus norvegicus 59-62 7544804-4 1995 Drug binding to gp330 was validated by equilibrium dialysis of [3H] gentamicin-gp330 incubations and binding/uptake studies in rat proximal tubules and gp330-expressing L2 carcinoma cells. Tritium 64-66 LDL receptor related protein 2 Rattus norvegicus 16-21 8095097-10 1993 Moreover, when the rat RNG alpha 2B-adrenoceptor was expressed in COS-7 cells and its binding properties tested using [3H]-MK 912 binding, guanoxabenz, LT 11 as well as a number of other drugs inhibited the ligand binding at a single alpha 2-adrenoceptor site; the drug Kds being practically the same as those found for the neonatal rat lung. Tritium 119-121 adrenoceptor alpha 2B Rattus norvegicus 27-48 7544804-4 1995 Drug binding to gp330 was validated by equilibrium dialysis of [3H] gentamicin-gp330 incubations and binding/uptake studies in rat proximal tubules and gp330-expressing L2 carcinoma cells. Tritium 64-66 LDL receptor related protein 2 Rattus norvegicus 79-84 8891613-0 1996 Characterisation of the specific binding of the histamine H3 receptor antagonist radioligand [3H]GR168320. Tritium 94-96 histamine receptor H3 Rattus norvegicus 48-69 7544804-4 1995 Drug binding to gp330 was validated by equilibrium dialysis of [3H] gentamicin-gp330 incubations and binding/uptake studies in rat proximal tubules and gp330-expressing L2 carcinoma cells. Tritium 64-66 LDL receptor related protein 2 Rattus norvegicus 79-84 7657637-2 1995 [3H]BK and [3H]NPC17731 bound with high affinity to the same B2 receptor in bovine myometrial membranes as determined by the total number of specific binding sites and pharmacological specificity of the binding of these two radioligands. Tritium 1-3 kininogen 1 Bos taurus 4-6 7657637-4 1995 All the heterobifunctional reagents plus the homobifunctional arylhalide 1,5-difluoro-2,4-dinitrobenzene were effective in cross-linking the [3H]BK N terminus specifically to a sulfhydryl in the receptor, and this cross-linking occurred at 5-100 microM reagent. Tritium 142-144 kininogen 1 Bos taurus 145-147 1334551-2 1992 An ammonium sulfate precipitate of mouse liver cytosol was purified by five chromatographic steps to isolate CBP in 14% yield and > 99% purity as monitored by [3H]cantharidin-binding activity. Tritium 163-165 CREB binding protein Mus musculus 109-112 1335877-2 1992 LTB4 and LY255283 displaced [3H]LTB4 from its binding site on lung membranes with pKi values of 9.9 and 7.0, respectively. Tritium 29-31 prostaglandin reductase 1 Cavia porcellus 0-4 1335877-2 1992 LTB4 and LY255283 displaced [3H]LTB4 from its binding site on lung membranes with pKi values of 9.9 and 7.0, respectively. Tritium 29-31 prostaglandin reductase 1 Cavia porcellus 32-36 1416973-5 1992 By saturation analysis, the concentration of specific binding sites for [3H]BP in PBP fraction from human liver was 4.6 pmol/mg of protein compared with 14.7 +/- 1.4 pmol/mg in the same fraction from DBA/2J mouse liver. Tritium 73-75 phosphatidylethanolamine binding protein 1 Homo sapiens 82-85 7562491-2 1995 ZM253,270 competitively inhibited [3H]NKA binding to native or cloned NK-2R from hamster urinary bladder (Ki = 2 nM), but was a weaker (48-fold) inhibitor of [3H]NKA binding to cloned human NK-2R. Tritium 35-37 tachykinin receptor 2 Homo sapiens 70-75 8748196-4 1995 LCAT activity was determined by conversion of 3H-cholesterol into 3H-esterified cholesterol. Tritium 46-48 lecithin cholesterol acyltransferase Rattus norvegicus 0-4 8584197-3 1995 Field electrical stimulation (FES) (2 Hz) induced an increase of dopamine release from striatal slices, which was Ca2+ dependent and was abolished by tetrodotoxin, 10(-6) M. Cholecystokinin octapeptide (10(-9) M, 10(-8) M and 10(-7) M) dose dependently reduced the electrically evoked release of [3H]DA. Tritium 297-299 cholecystokinin Rattus norvegicus 174-189 1426053-13 1992 An anti-CD44 monoclonal antibody, 50B4, was able to inhibit the binding of the [3H]HA to the U-373 MG cell line. Tritium 80-82 CD44 molecule (Indian blood group) Homo sapiens 8-12 8891613-6 1996 Histamine H3 receptor agonists and antagonists inhibited [3H]GR168320 binding with high affinity. Tritium 58-60 histamine receptor H3 Rattus norvegicus 0-21 8844469-2 1996 FCS, epidermal growth factor (EGF), and insulin significantly increased 5-bromo-2"-deoxyuridine (BrdU) uptake by the cells and EGF together with insulin increased the cells" [3H] thymidine uptake. Tritium 175-177 pro-epidermal growth factor Cavia porcellus 5-28 1408146-4 1992 H1 histamine receptors were identified in both v-K-ras- and v-H-ras-transformed Balb/3T3 cells, but not in untransformed cells, using the specific H1 antagonist [3H]-pyrilamine. Tritium 162-164 Harvey rat sarcoma virus oncogene Mus musculus 62-67 7646551-8 1995 This effect is reversed with a neutralizing antibody to lipocortin-1 (1 microgram/mL) suggesting that this protein mediates the inhibitory effects of glucocorticoids on agonist activated 3H-AA release. Tritium 187-189 annexin A1 Homo sapiens 56-68 7646551-10 1995 A peptide fragment from the N-terminus of lipocortin-1-Lc13-25 (20-200 micrograms/mL) mimics the effect of glucocorticoid in suppressing both EGF and G protein activated 3H-AA release. Tritium 170-172 annexin A1 Homo sapiens 42-54 8844469-2 1996 FCS, epidermal growth factor (EGF), and insulin significantly increased 5-bromo-2"-deoxyuridine (BrdU) uptake by the cells and EGF together with insulin increased the cells" [3H] thymidine uptake. Tritium 175-177 pro-epidermal growth factor Cavia porcellus 30-33 8844469-2 1996 FCS, epidermal growth factor (EGF), and insulin significantly increased 5-bromo-2"-deoxyuridine (BrdU) uptake by the cells and EGF together with insulin increased the cells" [3H] thymidine uptake. Tritium 175-177 pro-epidermal growth factor Cavia porcellus 127-130 7635662-4 1995 RESULTS: [3H]RX821002 bound to a single class of receptors with the characteristics of an alpha-2 adrenergic receptor with an affinity (KD) of 0.16 nM and a receptor density (Bmax) of 1500 fmol/mg protein. Tritium 10-12 adrenoceptor alpha 2A Bos taurus 90-117 8755535-5 1996 By preloading recombinant Ran/TC4 with [gamma-32P]GTP or [3H]GDP, we show that the interactions with p97 and NTF2 are specific for the GTP- and GDP-bound forms, respectively. Tritium 58-60 RAN, member RAS oncogene family Homo sapiens 26-29 7616236-2 1995 To show that the polypeptides detected on western blots and by immunoprecipitation represent the 5-HT2C receptor, binding studies of the 5-HT2C ligand [3H]-mesulergine to immunoprecipitates from extracts of pig choroid plexus were performed. Tritium 152-154 5-hydroxytryptamine receptor 2C Rattus norvegicus 97-103 8570860-9 1995 (2) IGF-II stimulates [3H]thy and LI but not cAMP; (3) IGF-I but not IGF-II effects are inhibited by SS14 and DDA. Tritium 23-25 insulin-like growth factor 2 Rattus norvegicus 4-10 1381560-6 1992 With double labeling, all [3H]thymidine-positive cells were PCNA positive, whereas some cells were only positive for PCNA. Tritium 27-29 proliferating cell nuclear antigen Rattus norvegicus 60-64 1358383-7 1992 Half of the compounds displacing [3H]-prazosin bound to guinea-pig cerebral cortex membranes display alpha 1A-adrenoceptor selectivity. Tritium 34-36 alpha-1A adrenergic receptor Cavia porcellus 101-122 8755535-5 1996 By preloading recombinant Ran/TC4 with [gamma-32P]GTP or [3H]GDP, we show that the interactions with p97 and NTF2 are specific for the GTP- and GDP-bound forms, respectively. Tritium 58-60 RAN, member RAS oncogene family Homo sapiens 30-33 1328044-3 1992 Equilibrium binding studies showed that peritoneal eosinophils bound [3H]LTB4 in a cell concentration and time-dependent fashion. Tritium 70-72 prostaglandin reductase 1 Cavia porcellus 73-77 8755535-5 1996 By preloading recombinant Ran/TC4 with [gamma-32P]GTP or [3H]GDP, we show that the interactions with p97 and NTF2 are specific for the GTP- and GDP-bound forms, respectively. Tritium 58-60 melanotransferrin Homo sapiens 101-104 8832062-15 1996 The competitive P-glycoprotein inhibitors, verapamil (100 microM), nifedipine (50 microM) and vinblastine (50 microM) all abolished net secretion of [3H]-digoxin due to both a decrease in Jb-a and an increase in Ja-b. Tritium 150-152 suppressor of cytokine signaling 1 Homo sapiens 212-216 1358117-5 1992 The affinity of [3H]SCH39166 for 5-HT2 and 5-HT1c receptors is at least an order of magnitude lower than the affinity of [3H]SCH23390 for these same receptor sites. Tritium 17-19 5-hydroxytryptamine receptor 2C Homo sapiens 43-49 7583242-0 1995 Learning-specific, time-dependent increase in [3H]phorbol dibutyrate binding to protein kinase C in selected regions of the rat brain. Tritium 47-49 protein kinase C, gamma Rattus norvegicus 80-96 8819077-2 1996 We investigated the effects of G-CSF (0.01 microgram/ml) and GM-CSF (0.01 microgram/ml) on [3H]thymidine (TdR) uptake, and the cytotoxicity of 1-beta-D-arabinofuranosylcytosine (ara-C) in leukemia cells from 17 pediatric patients. Tritium 92-94 colony stimulating factor 2 Homo sapiens 61-67 7583242-2 1995 Here we describe a rapid, specific and time-dependent increase in [3H]phorbol-12,13-dibutyrate ([3H]PDBu) binding to membrane-associated PKC in selected brain regions of rats submitted to an inhibitory avoidance task. Tritium 67-69 protein kinase C, gamma Rattus norvegicus 137-140 7583242-2 1995 Here we describe a rapid, specific and time-dependent increase in [3H]phorbol-12,13-dibutyrate ([3H]PDBu) binding to membrane-associated PKC in selected brain regions of rats submitted to an inhibitory avoidance task. Tritium 97-99 protein kinase C, gamma Rattus norvegicus 137-140 1292248-8 1992 We have found that the rate of [3H] thymidine incorporation corresponds to the elevated levels of c-fos and c-myc transcripts in the precancerous stages. Tritium 32-34 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 98-103 1507200-7 1992 Compound 17 also exhibited a long duration of action in the dog (inhibition of PAF-induced whole blood aggregation ex vivo was maintained for greater than 24 h following a single oral dose of 75 micrograms/kg) and was highly selective as a PAF antagonist, showing only weak affinity (IC50 = 6600 nM) for the [3H]nitrendipine binding site. Tritium 309-311 patchy fur Mus musculus 79-82 1327384-0 1992 [3H]-RS-15385-197, a selective and high affinity radioligand for alpha 2-adrenoceptors: implications for receptor classification. Tritium 1-3 adrenoceptor alpha 2A Homo sapiens 65-86 1327384-12 1992 alpha 2A-Adrenoceptor selective compounds, oxymetazoline and BRL 44409, showed high affinity for [3H]-RS-15385-197 binding in the human platelet and lower affinity in the neonate lung, while the alpha 2B-selective compounds, prazosin and imiloxan, showed high affinity for [3H]-RS-15385-197 binding in the neonate lung.This suggests that [3H]-RS-15385-197 labels both alpha2A- and alpha2B-adrenoceptor subtypes.4. Tritium 98-100 adrenoceptor alpha 2A Homo sapiens 0-21 1327384-12 1992 alpha 2A-Adrenoceptor selective compounds, oxymetazoline and BRL 44409, showed high affinity for [3H]-RS-15385-197 binding in the human platelet and lower affinity in the neonate lung, while the alpha 2B-selective compounds, prazosin and imiloxan, showed high affinity for [3H]-RS-15385-197 binding in the neonate lung.This suggests that [3H]-RS-15385-197 labels both alpha2A- and alpha2B-adrenoceptor subtypes.4. Tritium 274-276 adrenoceptor alpha 2A Homo sapiens 0-21 1327384-12 1992 alpha 2A-Adrenoceptor selective compounds, oxymetazoline and BRL 44409, showed high affinity for [3H]-RS-15385-197 binding in the human platelet and lower affinity in the neonate lung, while the alpha 2B-selective compounds, prazosin and imiloxan, showed high affinity for [3H]-RS-15385-197 binding in the neonate lung.This suggests that [3H]-RS-15385-197 labels both alpha2A- and alpha2B-adrenoceptor subtypes.4. Tritium 274-276 adrenoceptor alpha 2A Homo sapiens 0-21 1379560-3 1992 Addition of aFGF and heparin or bFGF alone to serum-free cultures of the dissociated Embryonic Day (E) 14.5 mesencephalon stimulates cell proliferation, as judged by [3H]thymidine autoradiography, leading to a maximal 75-fold increase in the total number of cells. Tritium 167-169 fibroblast growth factor 1 Rattus norvegicus 12-16 7612612-6 1995 In addition, for the Apol-3 PBP, displacement of [3H]-6E,11Z-16:Ac binding by other pheromone components or analogs showed a clear trend in relative binding affinity: 6E,11Z-16:Ac > 4E,9Z-14:Ac > 6E,11Z-16:Al approximately 16:Ac > 6E,11Z-16:OH > 4E,9Z-14:OH. Tritium 50-52 apolipoprotein L3 Homo sapiens 21-27 7612612-6 1995 In addition, for the Apol-3 PBP, displacement of [3H]-6E,11Z-16:Ac binding by other pheromone components or analogs showed a clear trend in relative binding affinity: 6E,11Z-16:Ac > 4E,9Z-14:Ac > 6E,11Z-16:Al approximately 16:Ac > 6E,11Z-16:OH > 4E,9Z-14:OH. Tritium 50-52 phosphatidylethanolamine binding protein 1 Homo sapiens 28-31 7589183-5 1995 These results indicate that [3H]sumatriptan labels predominantly 5-HT1D alpha and/or 5-HT1D beta (but not 5-HT1A or 5-HT1F) receptor subtypes in cat brain stem and spinal cord. Tritium 29-31 5-hydroxytryptamine receptor 1D Homo sapiens 65-77 7782298-9 1995 A 70% increase in IGF I receptor number following exposure to 5 microM sense oligonucleotide resulted in a 4-fold increase in basal [3H]thymidine incorporation, and angiotensin II (1-1000 nM) had no additive stimulatory effect. Tritium 133-135 insulin like growth factor 1 receptor Homo sapiens 18-32 1325151-6 1992 When [3H]inositol-loaded glomerulosa cells were stimulated with alpha-MSH there was significant generation of [3H]inositol trisphosphate (IP3) at concentrations of alpha-MSH which stimulated secretion of aldosterone. Tritium 6-8 proopiomelanocortin Rattus norvegicus 64-73 7758593-8 1995 AH23848B, a selective EP4 antagonist, not only displaced the [3H]PGE2 binding to the cloned receptor but antagonized the PGE2-stimulated cAMP formation in the receptor. Tritium 62-64 prostaglandin E receptor 4 (subtype EP4) Mus musculus 22-25 8660375-6 1996 Treatment with heparitinase which destroys heparan sulfate proteoglycan (HSPG) or with chlorate which inhibits sulfation of HSPG diminished [3H]thymidine incorporation of RGM1 cells in serum-free medium. Tritium 141-143 syndecan 1 Rattus norvegicus 124-128 1325151-6 1992 When [3H]inositol-loaded glomerulosa cells were stimulated with alpha-MSH there was significant generation of [3H]inositol trisphosphate (IP3) at concentrations of alpha-MSH which stimulated secretion of aldosterone. Tritium 6-8 proopiomelanocortin Rattus norvegicus 164-173 8764214-2 1996 Bradykinin induced a significant increase in [3H]glycoconjugate secretion in a dose-dependent manner from isolated glands, which was significantly inhibited by D-Arg-(Hyp3, Thi5,8, D-Phe7)-bradykinin (the B2-receptor antagonist), whereas Des-Arg9-(Leu8)-bradykinin (B1-receptor antagonist) or indomethacin did not significantly alter it. Tritium 46-48 bradykinin receptor B2 Homo sapiens 205-216 1353889-2 1992 mRNA synthesized from this clone (designated GLYT1) directs the expression of sodium- and chloride-dependent, high-affinity uptake of [3H]glycine by Xenopus oocytes. Tritium 135-137 solute carrier family 6 member 9 Rattus norvegicus 45-50 7744732-10 1995 These results strongly suggest that the MDR 3 homolog of P-gp is a CLM-binding protein and that direct interaction of Ca(2+)-CLM with P-gp, while not affecting its binding of [3H]VBL, down-modulates the translocation of this agent in the presence of ATP. Tritium 176-178 phosphoglycolate phosphatase Mus musculus 57-61 8764214-2 1996 Bradykinin induced a significant increase in [3H]glycoconjugate secretion in a dose-dependent manner from isolated glands, which was significantly inhibited by D-Arg-(Hyp3, Thi5,8, D-Phe7)-bradykinin (the B2-receptor antagonist), whereas Des-Arg9-(Leu8)-bradykinin (B1-receptor antagonist) or indomethacin did not significantly alter it. Tritium 46-48 selectin L Homo sapiens 248-252 1321029-1 1992 Distribution of the enzyme monoamine oxidase B (MAO-B) and the peripheral benzodiazepine binding site (omega 3 site) was studied by quantitative autoradiography using [3H]L-deprenyl and [3H]PK 11195, two tentative glial markers, as ligands. Tritium 168-170 monoamine oxidase B Homo sapiens 27-46 8818185-8 1996 In EGF- and TGF alpha-treated follicles, [3H]thymidine continued to be incorporated into DNA of the ORS and bulb after fibre growth ceased. Tritium 42-44 epidermal growth factor Homo sapiens 3-6 1321029-1 1992 Distribution of the enzyme monoamine oxidase B (MAO-B) and the peripheral benzodiazepine binding site (omega 3 site) was studied by quantitative autoradiography using [3H]L-deprenyl and [3H]PK 11195, two tentative glial markers, as ligands. Tritium 187-189 monoamine oxidase B Homo sapiens 27-46 7626704-6 1995 Moreover, PGE2 and, to a lesser extent, PGF2 alpha increased basal [3H]thymidine incorporation and enhanced DNA synthesis induced by a submaximal stimulatory concentration of TGF alpha. Tritium 68-70 transforming growth factor alpha Gallus gallus 175-184 8799564-8 1996 The beta 3-adrenoceptor was characterized by use of the new specific radioligand [3H]-SB 206606. Tritium 82-84 adrenoceptor beta 3 Homo sapiens 4-23 7613631-4 1995 Ex vivo studies demonstrated that 30 min after an intraventricular injection of 1 nmol/rat CCK-8 the KD value of [3H]NPA binding sites in the caudal part of the forebrain and the IC50 value of dopamine for [125I]iodosulpride binding sites in the caudal part of the nucleus accumbens were significantly increased by 160% and decreased by 77% respectively. Tritium 114-116 cholecystokinin Rattus norvegicus 91-94 1351857-3 1992 A strong correlation was observed between PCNA labeling index and both [3H]thymidine labeling index (R = 0.993, P = 0.007) and percent of cells in S phase as determined by flow cytometry (R = 0.982, P = 0.018) and between the location of the maximal staining for PCNA and [3H]thymidine (R = 0.997, P less than 0.05). Tritium 72-74 proliferating cell nuclear antigen Rattus norvegicus 42-46 1351857-3 1992 A strong correlation was observed between PCNA labeling index and both [3H]thymidine labeling index (R = 0.993, P = 0.007) and percent of cells in S phase as determined by flow cytometry (R = 0.982, P = 0.018) and between the location of the maximal staining for PCNA and [3H]thymidine (R = 0.997, P less than 0.05). Tritium 273-275 proliferating cell nuclear antigen Rattus norvegicus 42-46 1321099-4 1992 The 3He detection limit of the system is close to 10(-16) cm3 STP corresponding to a tritium level of 0.003 TU for a 500 g water sample stored six months for 3He regrowth. Tritium 85-92 thyroid hormone receptor interactor 10 Homo sapiens 62-65 1351684-7 1992 The existence of multiple neuronal 5HT1D-like receptors may help account for some of the complexities associated with [3H]serotonin binding patterns in native membranes. Tritium 119-121 5-hydroxytryptamine receptor 1D Homo sapiens 35-40 1375895-4 1992 Pretreatment with IGFBP-3 for 72 h resulted in a 2- to 4-fold augmentation of IGF-I-stimulated [3H]aminoisobutyric acid uptake; IGFBP-3 had no effect on basal [3H]aminoisobutyric acid uptake. Tritium 96-98 IGFI Bos taurus 78-83 1375895-4 1992 Pretreatment with IGFBP-3 for 72 h resulted in a 2- to 4-fold augmentation of IGF-I-stimulated [3H]aminoisobutyric acid uptake; IGFBP-3 had no effect on basal [3H]aminoisobutyric acid uptake. Tritium 96-99 IGFI Bos taurus 78-83 8586645-5 1995 Substrates and inhibitors of debrisoquine 4-hydroxylase potently displace [3H]-mepyramine binding to MBP. Tritium 75-79 cytochrome P450, family 2, subfamily d, polypeptide 1 Rattus norvegicus 29-55 8586645-5 1995 Substrates and inhibitors of debrisoquine 4-hydroxylase potently displace [3H]-mepyramine binding to MBP. Tritium 75-79 myelin basic protein Rattus norvegicus 101-104 7751909-7 1995 In addition, both noxious chemical and thermal stimulation produced significant increases in specific 3H-PDBu binding in the dorsal horn of the lumbar spinal cord, likely reflecting alterations in membrane-associated PKC. Tritium 102-104 protein kinase C, gamma Rattus norvegicus 217-220 8645989-4 1996 Excess unlabeled PAF (500 nM) or the specific PAF receptor antagonist WEB 2086 (1 microM) inhibited the [3H]PAF binding. Tritium 105-107 PCNA clamp associated factor Homo sapiens 17-20 1602382-2 1992 Therefore, the present investigations use in vivo techniques to measure the rate at which [3H]rCD4 is transported through the blood-brain barrier (BBB). Tritium 91-93 Cd4 molecule Rattus norvegicus 94-98 1602382-4 1992 These studies show that [3H]CD4 is removed rapidly from the bloodstream with a half-time of 12.6 +/- 0.9 min. Tritium 25-27 Cd4 molecule Rattus norvegicus 28-31 1602382-6 1992 In addition, [3H]rCD4 is extracted rapidly by the kidney and the ratio of rCD4 Vd to native rat serum albumin Vd in the rat kidney reaches 99 +/- 5 at 60 min after i.v. Tritium 14-16 Cd4 molecule Rattus norvegicus 17-21 7742388-5 1995 The receptor affinities (alpha 2-C10) were highest in glycylglycine, almost equally high in K(+)-phosphate, and lowest in Tris buffer for all three [3H]-ligands. Tritium 149-151 adrenoceptor alpha 2A Homo sapiens 25-36 8645989-4 1996 Excess unlabeled PAF (500 nM) or the specific PAF receptor antagonist WEB 2086 (1 microM) inhibited the [3H]PAF binding. Tritium 105-107 PCNA clamp associated factor Homo sapiens 46-49 1602382-6 1992 In addition, [3H]rCD4 is extracted rapidly by the kidney and the ratio of rCD4 Vd to native rat serum albumin Vd in the rat kidney reaches 99 +/- 5 at 60 min after i.v. Tritium 14-16 Cd4 molecule Rattus norvegicus 74-78 8645989-4 1996 Excess unlabeled PAF (500 nM) or the specific PAF receptor antagonist WEB 2086 (1 microM) inhibited the [3H]PAF binding. Tritium 105-107 PCNA clamp associated factor Homo sapiens 46-49 7747433-4 1995 Release of the 3H-label by alkaline methanolysis suggests that the palmitate was associated with nsP1 via an ester bond. Tritium 15-17 SH2 domain containing 3A Homo sapiens 97-101 8639640-9 1996 [3H]Oleic acid bound specifically to recombinant soluble uPAR in a 1:1 molar ratio in the presence or absence of plasma and totally blocked its specific binding to a cell line expressing glycosyl phosphatidylinositol-linked single chain urokinase. Tritium 1-3 plasminogen activator, urokinase receptor Homo sapiens 57-61 1614411-0 1992 [3H]pBC 264, a suitable probe for studying cholecystokinin-B receptors: binding characteristics in rodent brains and comparison with [3H]SNF 8702. Tritium 1-3 cholecystokinin Mus musculus 43-58 1614411-2 1992 The concentration of CCK-A receptors in rodent brain was estimated to be 8-10 fmol/mg of protein, by measurement of the Bmax values of the nonselective agonist [3H] propionyl-CCK8, with or without 10 nM pBC 264 to saturate CCK-B sites. Tritium 161-163 cholecystokinin Mus musculus 21-24 1614411-2 1992 The concentration of CCK-A receptors in rodent brain was estimated to be 8-10 fmol/mg of protein, by measurement of the Bmax values of the nonselective agonist [3H] propionyl-CCK8, with or without 10 nM pBC 264 to saturate CCK-B sites. Tritium 161-163 cholecystokinin Mus musculus 175-178 8639530-3 1996 Binding of EGF was followed by sedimentation of the complex between [3H]EGF and EGF receptor, dimerization was measured by quantitative cross-linking with glutaraldehyde, and the activation of the protein tyrosine kinase was monitored under the same conditions by following the initial velocity of the phosphorylation of peptides containing tyrosine. Tritium 69-71 epidermal growth factor Homo sapiens 11-14 8574778-3 1995 When 10 nM ANF was added to the medium containing KCl plus ANG II or KCl plus ANG III, the reduction of 3H-NE output by ANF was greater than when the atrial factor was added to the medium containing only ANG II or ANG III. Tritium 104-106 natriuretic peptide A Rattus norvegicus 11-14 8574778-3 1995 When 10 nM ANF was added to the medium containing KCl plus ANG II or KCl plus ANG III, the reduction of 3H-NE output by ANF was greater than when the atrial factor was added to the medium containing only ANG II or ANG III. Tritium 104-106 angiogenin Rattus norvegicus 59-62 8574778-3 1995 When 10 nM ANF was added to the medium containing KCl plus ANG II or KCl plus ANG III, the reduction of 3H-NE output by ANF was greater than when the atrial factor was added to the medium containing only ANG II or ANG III. Tritium 104-106 natriuretic peptide A Rattus norvegicus 120-123 8574778-3 1995 When 10 nM ANF was added to the medium containing KCl plus ANG II or KCl plus ANG III, the reduction of 3H-NE output by ANF was greater than when the atrial factor was added to the medium containing only ANG II or ANG III. Tritium 104-106 angiogenin Rattus norvegicus 78-81 8621160-8 1996 Moreover, a commonly used phospholipase A2 (PLA2) inhibitor, quinacrine, decreased HGF-induced [3H]AA release and [3H]thymidine incorporation. Tritium 96-98 phospholipase A2 group IB Rattus norvegicus 26-42 7876220-4 1995 Recombinant wild type and mutant CRABP-II proteins were expressed and purified, and the affinity for retinoids was determined by fluorometric titration and binding of 3H-labeled compounds. Tritium 167-169 cellular retinoic acid binding protein 2 Homo sapiens 33-41 1317924-5 1992 Optimization of the N- and O-substituents resulted in the analogues 25-27 which exhibited Ki values ranging between 131 and 167 nM in a [3H]PAF binding assay. Tritium 137-139 patchy fur Mus musculus 140-143 1533571-8 1992 The basal rate of protein synthesis, as determined by [3H]leucine incorporation, was approximately 50% higher in the CD44hi small CD4+ T cells than in the CD44lo CD4+ T cells. Tritium 55-57 CD44 molecule (Indian blood group) Homo sapiens 117-121 8621160-8 1996 Moreover, a commonly used phospholipase A2 (PLA2) inhibitor, quinacrine, decreased HGF-induced [3H]AA release and [3H]thymidine incorporation. Tritium 96-98 phospholipase A2 group IB Rattus norvegicus 44-48 1324864-2 1992 The peripheral-type benzodiazepine receptor was characterized in rat prostate and seminal vesicle using [3H]Ro 5-4864 as radioligand. Tritium 105-107 translocator protein Rattus norvegicus 4-43 1378422-4 1992 Of the three clones producing antibodies which bound to LTB4-R, 0.5 mg/ml of one IgG3k antibody, termed E2, precipitated over 90% of the [3H]LTB4-binding activity of solubilized PMN leucocyte membrane proteins. Tritium 138-140 leukotriene B4 receptor Homo sapiens 56-62 1573630-6 1992 These compounds decrease [3H]thymidine incorporation in AEBS-containing EL4 lymphoid cells and MCF7 breast cancer cells in a concentration-dependent manner between 10(-8) and 10(-6) M and are generally more inhibitory than tamoxifen. Tritium 26-28 epilepsy 4 Mus musculus 72-75 7773187-5 1995 Methaemoglobin (MetHb) also inhibited both [3H]GDP-dissociation and [32P]GTP-hydrolysis activities of ram p25 in a very similar manner to Hb. Tritium 44-46 tubulin polymerization promoting protein Homo sapiens 106-109 7540095-13 1995 [3H]-ryanodine binding to HSR was suppressed by ruthenium red, Mg2+ and procaine, but was not affected by DBHC up to 100 microM. Tritium 1-3 HSR Homo sapiens 26-29 7540095-15 1995 [3H]-ryanodine binding to HSR was enhanced by caffeine and BED. Tritium 1-3 HSR Homo sapiens 26-29 7540095-18 1995 9-[3H]-Methyl-7-bromo-eudistomin D, an 3H-labelled analogue of BED, specifically bound to HSR. Tritium 3-5 HSR Homo sapiens 104-107 8621160-8 1996 Moreover, a commonly used phospholipase A2 (PLA2) inhibitor, quinacrine, decreased HGF-induced [3H]AA release and [3H]thymidine incorporation. Tritium 115-117 phospholipase A2 group IB Rattus norvegicus 26-42 8621160-8 1996 Moreover, a commonly used phospholipase A2 (PLA2) inhibitor, quinacrine, decreased HGF-induced [3H]AA release and [3H]thymidine incorporation. Tritium 115-117 phospholipase A2 group IB Rattus norvegicus 44-48 8627566-3 1996 In vitro functional assays are consistent with potent competitive antagonism of substance P-(SP) or neurokinin A-(NKA) induced [3H]-inositol phosphate accumulation in NK-1 or NK-2 monoreceptor cell lines with pA2 values of 8.19 and 8.67, respectively. Tritium 128-130 tachykinin receptor 1 Homo sapiens 167-171 7885273-2 1995 In adipocytes, insulin-stimulated [3H]2-DOG uptake was decreased by prior 60-minute treatment with dexamethasone (DEX) or prednisolone (PSL), whereas [125I]insulin binding, insulin (INS) receptor autophosphorylation, and tyrosine kinase activity, as measured using exogenous substrate of poly(Glu80-Tyr20), were not significantly changed. Tritium 35-37 insulin Canis lupus familiaris 15-22 8634092-1 1996 Gene-specific DNA damage levels were determined by quantitative polymerase chain reaction (QPCR) after treating cytochrome P450 (CYP) 1A1-expressing xeroderma pigmentosum fibroblasts with [3H]benzo[a]pyrene-trans-7,8-dihydrodiol ([3H]BPD) or [3H]benzo[a]pyrene-trans-7,8-dihydrodiol-9,10-epoxide ([3H]BPDE). Tritium 189-191 cytochrome P450 family 1 subfamily A member 1 Homo sapiens 112-137 7814408-11 1995 In addition, PLA2 treated with [3H]BEL resulted in the covalent labeling of a major band at M(r) 80,000. Tritium 32-34 phospholipase A2, group IB, pancreas Mus musculus 13-17 7814408-12 1995 Inactivation of the PLA2 by 5,5"-dithiobis(2-nitrobenzoic acid) prior to treatment with [3H]BEL resulted in the near complete lack of labeling consistent with covalent irreversible suicide inhibition of the enzyme. Tritium 89-91 phospholipase A2, group IB, pancreas Mus musculus 20-24 8634092-1 1996 Gene-specific DNA damage levels were determined by quantitative polymerase chain reaction (QPCR) after treating cytochrome P450 (CYP) 1A1-expressing xeroderma pigmentosum fibroblasts with [3H]benzo[a]pyrene-trans-7,8-dihydrodiol ([3H]BPD) or [3H]benzo[a]pyrene-trans-7,8-dihydrodiol-9,10-epoxide ([3H]BPDE). Tritium 231-233 cytochrome P450 family 1 subfamily A member 1 Homo sapiens 112-137 7814408-13 1995 The labeling of a M(r) 80,000 band rather than a M(r) 40,000 band upon treatment with [3H]BEL distinguishes the macrophage Ca(2+)-independent PLA2 from a previously identified myocardial Ca(2+)-independent PLA2 and provides strong evidence that the M(r) 80,000 protein is the catalytic subunit. Tritium 87-89 phospholipase A2, group IB, pancreas Mus musculus 142-146 8795068-6 1996 IGF-I and factors from serum of hypophysectomized rats (HRS) stimulated [3H]thymidine incorporation into DNA of NRKE cells. Tritium 73-75 insulin-like growth factor 1 Rattus norvegicus 0-5 7537428-4 1995 In addition, recombinant human (rh) SCF was tested in [3H]thymidine uptake assays for a mitogenic effect on these cells. Tritium 55-57 KIT ligand Homo sapiens 36-39 8738477-8 1996 In general, the increase in enzyme activity correlated with cell proliferation as measured by [3H]thymidine uptake in PHA-stimulated cultures containing IL-2 and/or 2-ME. Tritium 95-97 interleukin 2 Rattus norvegicus 153-157 8773255-5 1995 In addition, slices at CD 10-20 expressed a low to high affinity [3H]AMPA binding ratio that was comparable with that in the adult hippocampus (10:1). Tritium 66-68 membrane metallo-endopeptidase Rattus norvegicus 23-28 8601217-2 1996 Mitogenic pairs of anti-CD2 monoclonal antibodies inhibited [3H]thymidine incorporation from 25 to 62% in CD2+ HTLV-I-infected lymphocytes. Tritium 61-63 CD2 molecule Homo sapiens 24-27 7715741-2 1995 We studied the effects of prostaglandin E2 (PGE2), prostaglandin D2 (PGD2) and related drugs on the electrically evoked (50 mA, 2 ms, 0.3 Hz) tritium overflow. Tritium 142-149 prostaglandin D2 synthase (brain) Mus musculus 51-67 7715741-2 1995 We studied the effects of prostaglandin E2 (PGE2), prostaglandin D2 (PGD2) and related drugs on the electrically evoked (50 mA, 2 ms, 0.3 Hz) tritium overflow. Tritium 142-149 prostaglandin D2 synthase (brain) Mus musculus 69-73 8601217-2 1996 Mitogenic pairs of anti-CD2 monoclonal antibodies inhibited [3H]thymidine incorporation from 25 to 62% in CD2+ HTLV-I-infected lymphocytes. Tritium 61-63 CD2 molecule Homo sapiens 106-109 7703435-0 1994 PAF antagonist, BN52021, inhibits [3H]D-aspartate release after ischaemia in vitro. Tritium 35-37 PCNA clamp associated factor Rattus norvegicus 0-3 8615756-3 1996 [3H]Thymidine autoradiography was used to map the time frame of PAI-1 expression during a synchronous growth cycle. Tritium 1-3 serpin family E member 2 Rattus norvegicus 64-69 7983045-8 1994 This time course appears to represent tandem binding of [3H]VBL by P-gp and its mediated transport, with the latter process representing the rate-limiting step. Tritium 57-59 phosphoglycolate phosphatase Mus musculus 67-71 8626384-9 1996 These data show that cPLA2 plays a major role in [3H]arachidonic acid release by MCP-1 in human monocytes and provide direct evidence for the involvement of cPLA2 in C-C chemokine-induced monocyte chemotaxis. Tritium 50-52 C-C motif chemokine ligand 2 Homo sapiens 81-86 7964734-2 1994 Endogenous PAF levels, determined by a bioassay using PAF-stimulated platelet release of [3H]serotonin, averaged 2.32 +/- 2.14 pg/mg in control brains and was reduced to 1.10 +/- 1.06 pg/mg after 20 min of maternal-fetal blood flow occlusion. Tritium 90-92 PCNA clamp associated factor Rattus norvegicus 11-14 7964734-2 1994 Endogenous PAF levels, determined by a bioassay using PAF-stimulated platelet release of [3H]serotonin, averaged 2.32 +/- 2.14 pg/mg in control brains and was reduced to 1.10 +/- 1.06 pg/mg after 20 min of maternal-fetal blood flow occlusion. Tritium 90-92 PCNA clamp associated factor Rattus norvegicus 54-57 8626410-16 1996 Photolabeling of mitochondria with [3H]AzDA resulted in a prominent 32 kDa band of UCP, and few other proteins were labeled. Tritium 36-38 uncoupling protein 1 Homo sapiens 83-86 7964734-3 1994 [3H]PAF administered intracranially into the fetuses in utero was removed in a biphasic, time-dependent manner: a rapid component with an estimated elimination rate constant of 0.067 min-1 and t1/2 of 10 min and a slower component with an elimination rate of 0.017 min-1 and t1/2 of 41 min. Tritium 1-3 PCNA clamp associated factor Rattus norvegicus 4-7 7964734-4 1994 In fetal brains subjected to ischemia a delayed elimination of [3H]PAF was noticed in the slow component (t1/2 = 59 min), indicating a possible difference between the clearance of exogenous and endogenous PAF. Tritium 64-66 PCNA clamp associated factor Rattus norvegicus 67-70 7964734-4 1994 In fetal brains subjected to ischemia a delayed elimination of [3H]PAF was noticed in the slow component (t1/2 = 59 min), indicating a possible difference between the clearance of exogenous and endogenous PAF. Tritium 64-66 PCNA clamp associated factor Rattus norvegicus 205-208 7964734-8 1994 Addition of the calcium ionophore A23187 stimulated in vitro formation of PAF and lyso-PAF from [3H]-choline-labeled fetal brain phospholipids, suggesting that intracellular calcium may play a major stimulatory role in PAF production. Tritium 97-99 PCNA clamp associated factor Rattus norvegicus 74-77 7964734-8 1994 Addition of the calcium ionophore A23187 stimulated in vitro formation of PAF and lyso-PAF from [3H]-choline-labeled fetal brain phospholipids, suggesting that intracellular calcium may play a major stimulatory role in PAF production. Tritium 97-99 PCNA clamp associated factor Rattus norvegicus 87-90 7964734-8 1994 Addition of the calcium ionophore A23187 stimulated in vitro formation of PAF and lyso-PAF from [3H]-choline-labeled fetal brain phospholipids, suggesting that intracellular calcium may play a major stimulatory role in PAF production. Tritium 97-99 PCNA clamp associated factor Rattus norvegicus 87-90 7996484-4 1994 The potent cocaine congener [3H]WIN 35,428 ((CFT), 2B-carbomethoxy-3 beta-(4-fluorophenyl)-tropane) has been shown to recognize high and low affinity binding sites on the DA transporter. Tritium 29-31 solute carrier family 6 member 3 Homo sapiens 171-185 7996484-5 1994 We have used [3H]WIN 35,428 to map and quantify the high affinity cocaine recognition site on the DA transporter in victims of fatal cocaine overdose. Tritium 14-16 solute carrier family 6 member 3 Homo sapiens 98-112 7980444-7 1994 We determined that smgGDS prevented the loss of Rac1 activity during the [3H]GDP/GTP exchange assay by demonstrating the ability of smgGDS to inhibit the loss of Rac1 GTP[gamma S]-binding during incubations at 30 degrees C. This stabilizing effect was exactly counterbalanced by the ability of smgGDS to stimulate the release of [3H]GDP from Rac1, thereby producing no net observable effect in the exchange assay. Tritium 74-76 Rap1 GTPase-GDP dissociation stimulator 1 Homo sapiens 19-25 7980444-7 1994 We determined that smgGDS prevented the loss of Rac1 activity during the [3H]GDP/GTP exchange assay by demonstrating the ability of smgGDS to inhibit the loss of Rac1 GTP[gamma S]-binding during incubations at 30 degrees C. This stabilizing effect was exactly counterbalanced by the ability of smgGDS to stimulate the release of [3H]GDP from Rac1, thereby producing no net observable effect in the exchange assay. Tritium 74-76 Rac family small GTPase 1 Homo sapiens 48-52 7980444-7 1994 We determined that smgGDS prevented the loss of Rac1 activity during the [3H]GDP/GTP exchange assay by demonstrating the ability of smgGDS to inhibit the loss of Rac1 GTP[gamma S]-binding during incubations at 30 degrees C. This stabilizing effect was exactly counterbalanced by the ability of smgGDS to stimulate the release of [3H]GDP from Rac1, thereby producing no net observable effect in the exchange assay. Tritium 74-76 Rap1 GTPase-GDP dissociation stimulator 1 Homo sapiens 132-138 8829620-2 1996 The treatment of cells with EGF resulted in an increase in [3H]thymidine incorporation into DNA depending upon EGF concentrations. Tritium 60-62 epidermal growth factor Homo sapiens 28-31 7980444-7 1994 We determined that smgGDS prevented the loss of Rac1 activity during the [3H]GDP/GTP exchange assay by demonstrating the ability of smgGDS to inhibit the loss of Rac1 GTP[gamma S]-binding during incubations at 30 degrees C. This stabilizing effect was exactly counterbalanced by the ability of smgGDS to stimulate the release of [3H]GDP from Rac1, thereby producing no net observable effect in the exchange assay. Tritium 74-76 Rap1 GTPase-GDP dissociation stimulator 1 Homo sapiens 132-138 7980444-7 1994 We determined that smgGDS prevented the loss of Rac1 activity during the [3H]GDP/GTP exchange assay by demonstrating the ability of smgGDS to inhibit the loss of Rac1 GTP[gamma S]-binding during incubations at 30 degrees C. This stabilizing effect was exactly counterbalanced by the ability of smgGDS to stimulate the release of [3H]GDP from Rac1, thereby producing no net observable effect in the exchange assay. Tritium 330-332 Rap1 GTPase-GDP dissociation stimulator 1 Homo sapiens 19-25 7980444-7 1994 We determined that smgGDS prevented the loss of Rac1 activity during the [3H]GDP/GTP exchange assay by demonstrating the ability of smgGDS to inhibit the loss of Rac1 GTP[gamma S]-binding during incubations at 30 degrees C. This stabilizing effect was exactly counterbalanced by the ability of smgGDS to stimulate the release of [3H]GDP from Rac1, thereby producing no net observable effect in the exchange assay. Tritium 330-332 Rac family small GTPase 1 Homo sapiens 48-52 7980444-7 1994 We determined that smgGDS prevented the loss of Rac1 activity during the [3H]GDP/GTP exchange assay by demonstrating the ability of smgGDS to inhibit the loss of Rac1 GTP[gamma S]-binding during incubations at 30 degrees C. This stabilizing effect was exactly counterbalanced by the ability of smgGDS to stimulate the release of [3H]GDP from Rac1, thereby producing no net observable effect in the exchange assay. Tritium 330-332 Rap1 GTPase-GDP dissociation stimulator 1 Homo sapiens 132-138 7980444-7 1994 We determined that smgGDS prevented the loss of Rac1 activity during the [3H]GDP/GTP exchange assay by demonstrating the ability of smgGDS to inhibit the loss of Rac1 GTP[gamma S]-binding during incubations at 30 degrees C. This stabilizing effect was exactly counterbalanced by the ability of smgGDS to stimulate the release of [3H]GDP from Rac1, thereby producing no net observable effect in the exchange assay. Tritium 330-332 Rap1 GTPase-GDP dissociation stimulator 1 Homo sapiens 132-138 8829620-2 1996 The treatment of cells with EGF resulted in an increase in [3H]thymidine incorporation into DNA depending upon EGF concentrations. Tritium 60-62 epidermal growth factor Homo sapiens 111-114 8769871-0 1996 [3H]LY303870, a novel nonpeptide radioligand for the NK-1 receptor. Tritium 1-3 substance-P receptor Cavia porcellus 53-66 8001077-4 1994 Under low stringency hybridization washing, human beta 2-adrenoceptor oligonucleotide probe signals were detected in granular cells located in those areas of polyp endoderm that were labelled by [3H]-(+/-)CGP12177. Tritium 196-198 adrenoceptor beta 2 Homo sapiens 50-69 8611021-3 1996 In vitro translation of the mRNA coding for this fusion cDNA using rabbit reticulocyte lysate gave rise to an N-myristoylated fusion TNF with a molecular mass of 18 kDa as determined by the incorporation of [3H]myristic acid and by immunoprecipitation with anti-TNF antibody. Tritium 208-210 tumor necrosis factor Oryctolagus cuniculus 133-136 7931326-2 1994 PAF inhibited K+ depolarization-induced [3H]ACh release in slices of brain cortex and hippocampus by up to 59% at 10 nM but did not inhibit [3H]ACh release in striatal slices. Tritium 41-43 PCNA clamp associated factor Rattus norvegicus 0-3 7931326-4 1994 The inhibition of K(+)-evoked [3H]ACh release induced by PAF was prevented by pretreating tissues with several structurally different PAF receptor antagonists. Tritium 30-34 PCNA clamp associated factor Rattus norvegicus 57-60 7931326-4 1994 The inhibition of K(+)-evoked [3H]ACh release induced by PAF was prevented by pretreating tissues with several structurally different PAF receptor antagonists. Tritium 30-34 PCNA clamp associated factor Rattus norvegicus 134-137 7931326-6 1994 PAF-induced inhibition of [3H]ACh release was blocked 90 +/- 3 and 86 +/- 2% by pertussis toxin and by anti-G alpha i1/2 antiserum incorporated into cortical synaptosomes, respectively. Tritium 27-29 PCNA clamp associated factor Rattus norvegicus 0-3 7969072-9 1994 The 293 cells transfected with either the alpha 1Q10 or alpha 1Q110 mutant in combination with beta 1 and gamma 2 subunits expressed significantly lower levels of [3H]Ro15-1788 binding, relative to WT levels. Tritium 164-166 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 Homo sapiens 95-113 7855732-4 1994 Exogenous 5-HT and the 5-HT1B agonist, CGS-12066B, both decreased the K(+)-stimulated release of [3H]5-HT. Tritium 98-100 5-hydroxytryptamine receptor 1B Rattus norvegicus 23-29 8593790-8 1996 When activin (1 ng/ml), but not inhibin, was present with FSH, the gonadotropin caused dose-dependent increases in [3H]DNA synthesis over a wide range of FSH concentrations (1-100 ng/ml). Tritium 116-118 inhibin subunit beta E Homo sapiens 5-12 7855732-5 1994 A 5-HT1B antagonist (methiothepin) blocked the 5-HT- and the CGS-12066B-induced inhibition of K(+)-evoked release of [3H]5-HT. Tritium 118-120 5-hydroxytryptamine receptor 1B Rattus norvegicus 2-8 7855732-9 1994 The 5-HT receptor subtype involved in the inhibition of the evoked release of [3H]5-HT is of the 5-HT1B subtype. Tritium 79-81 5-hydroxytryptamine receptor 1B Rattus norvegicus 97-103 1576704-3 1992 The results demonstrated that both purified soybean lipoxygenase and guinea-pig tissue cytosolic lipoxygenases were able to activate AFB1 to form [3H]AFB1-DNA adduct(s). Tritium 147-149 linoleate 9S-lipoxygenase-4 Glycine max 52-64 8592120-9 1996 The stimulation by IGF-I of [3H]thymidine incorporation was attenuated by ouabain and a low external K+ level. Tritium 29-31 insulin-like growth factor 1 Rattus norvegicus 19-24 1548463-3 1992 The receptor density of this site (Bmax, 72 fmol/mg of protein) matched that of the 5-HT1B receptor determined with [3H]5-HT. Tritium 117-119 5-hydroxytryptamine receptor 1B Rattus norvegicus 84-90 1548463-5 1992 The rank order of their binding affinities with this new radioligand showed a high degree of correlation with their affinities at the 5-HT1B receptor determined with [3H]5-HT or [125I]iodocyanopindolol. Tritium 167-169 5-hydroxytryptamine receptor 1B Rattus norvegicus 134-140 1548463-9 1992 The results of this study suggest that [3H]CP-96,501 is a useful agonist radioligand for the 5-HT1B receptor. Tritium 40-42 5-hydroxytryptamine receptor 1B Rattus norvegicus 93-99 7865767-6 1994 Competition of [3H]U69,593 binding to the KOR by bremazocine, alpha-neoendorphin, fedotozine and oxymorphindole resolved a single class of binding sites at which these agents had binding affinities similar to that of the kappa 1b site present in mouse brain. Tritium 16-18 opioid receptor, kappa 1 Mus musculus 42-45 7865767-6 1994 Competition of [3H]U69,593 binding to the KOR by bremazocine, alpha-neoendorphin, fedotozine and oxymorphindole resolved a single class of binding sites at which these agents had binding affinities similar to that of the kappa 1b site present in mouse brain. Tritium 16-18 prodynorphin Mus musculus 62-80 8592140-6 1996 The alpha 1 subunit contributes the high-affinity binding of [3H]Ro 15-1788 (flumazenil) and the diazepam-sensitive binding of [3H]Ro 15-4513. Tritium 62-64 adrenoceptor alpha 1D Homo sapiens 4-11 7524683-1 1994 By measuring binding of [125I]CCK-8 and [3H]L-364,718 to rat pancreatic acini we demonstrated directly that the pancreatic CCK receptor can exist in three different affinity states with respect to CCK--high affinity, low affinity and very low affinity. Tritium 41-43 cholecystokinin Rattus norvegicus 123-126 7524683-1 1994 By measuring binding of [125I]CCK-8 and [3H]L-364,718 to rat pancreatic acini we demonstrated directly that the pancreatic CCK receptor can exist in three different affinity states with respect to CCK--high affinity, low affinity and very low affinity. Tritium 41-43 cholecystokinin Rattus norvegicus 123-126 1377629-1 1992 The ability of atrial natriuretic peptide (ANP) to modulate K+-stimulated release of [3H]norepinephrine ([3H]NE) from rat hypothalamic slices was investigated. Tritium 86-88 natriuretic peptide A Rattus norvegicus 15-41 8592140-6 1996 The alpha 1 subunit contributes the high-affinity binding of [3H]Ro 15-1788 (flumazenil) and the diazepam-sensitive binding of [3H]Ro 15-4513. Tritium 128-130 adrenoceptor alpha 1D Homo sapiens 4-11 7929202-2 1994 A novel hepatoma-derived growth factor was purified from the conditioned medium of human hepatoma-derived cell line, HuH-7, by the assay of [3H]thymidine incorporation into Swiss 3T3 cells. Tritium 141-143 MIR7-3 host gene Homo sapiens 117-122 1544468-2 1992 Purified human pancreatic lipase was covalently modified by incubation with [3H], [14C] tetrahydrolipstatin (THL), a potent inhibitor of pancreatic lipase. Tritium 77-79 pancreatic lipase Homo sapiens 15-32 8624843-14 1996 Blood cardiolipin competes [3H]PAF binding in rabbit platelets. Tritium 28-30 PCNA clamp associated factor Homo sapiens 31-34 1544468-2 1992 Purified human pancreatic lipase was covalently modified by incubation with [3H], [14C] tetrahydrolipstatin (THL), a potent inhibitor of pancreatic lipase. Tritium 77-79 pancreatic lipase Homo sapiens 137-154 1371994-2 1992 TNF alpha stimulated the release of 3H radioactivity from intact cells labeled with [3H]arachidonic acid within 10 min in a dose dependent manner and the production of lyso forms of phospholipids, an event presumably mediated through the activation of phospholipase A2. Tritium 36-38 phospholipase A2, group IB, pancreas Mus musculus 252-268 1371281-3 1992 Acid hydrolysis of CK8 and CK18, purified from [3H]glucosamine-labeled cells, generated free glucosamine. Tritium 48-50 keratin 8 Homo sapiens 19-22 7840424-7 1994 Autoradiographic localization of PBR obtained by incubating ovary sections with [3H] PK11195, a ligand selective for PBR, revealed the presence of specific labelling in all the steroid-secreting cells. Tritium 81-83 translocator protein Rattus norvegicus 33-36 7840424-7 1994 Autoradiographic localization of PBR obtained by incubating ovary sections with [3H] PK11195, a ligand selective for PBR, revealed the presence of specific labelling in all the steroid-secreting cells. Tritium 81-83 translocator protein Rattus norvegicus 117-120 8646598-8 1996 IGF-II stimulated [3H]thymidine incorporation into DNA in DU145 and PC-3 cells significantly although the effect was small. Tritium 19-21 insulin like growth factor 2 Homo sapiens 0-6 7868185-9 1994 Most important, in C6trk+ cells, NGF was a weaker (2-fold) inducer of [3H]thymidine incorporation when compared to bFGF (5-fold), suggesting that expression of trkA fails to confer to NGF a strong mitogenic effect. Tritium 71-73 nerve growth factor Mus musculus 33-36 1317702-8 1992 Stereospecific binding (SSB) of 3H-M was evaluated with respect to: 1) the effect of naloxone treatment in vivo on 3H-M binding in vitro, 2) the effect of morphine treatment in vivo on 3H-M binding in vitro; and 3) the differences between 3H-M bound (in vitro) and both naloxone and morphine treatment (in vivo). Tritium 32-34 Sjogren syndrome antigen B Mus musculus 24-27 1729390-8 1992 Sucrose gradient analysis of brain ADA revealed only one corresponding peak of activity and [3H]DCF-labeled ADA in DCF-treated and control rats. Tritium 93-95 adenosine deaminase Rattus norvegicus 35-38 8637428-7 1996 In addition, 5-azacytidine, bromodeoxyuridine, leukemia inhibitory factor, and N-2 substantially increased [3H]acetylcholine levels, and these increases correlated highly with increases in total acetylcholine levels. Tritium 108-110 LIF interleukin 6 family cytokine Homo sapiens 47-73 1729390-8 1992 Sucrose gradient analysis of brain ADA revealed only one corresponding peak of activity and [3H]DCF-labeled ADA in DCF-treated and control rats. Tritium 93-95 adenosine deaminase Rattus norvegicus 108-111 1738111-7 1992 Similar to the pharmacological characteristics of the 5HT1B site defined by [125I]ICYP, compounds most active at inhibiting 15 mM K(+)-stimulated release of [3H]-5-HT were RU24969 = 5-carboxyamidotryptamine HCl = CGS 12066B greater than 5-methoxytryptamine greater than 5-HT. Tritium 158-160 5-hydroxytryptamine receptor 1B Rattus norvegicus 54-59 1738111-9 1992 A correlation analysis of selective 5-HT1B compounds comparing the pKD for displacement of [125I]ICYP vs. the IC50 for inhibition of [3H]-5-HT release demonstrated the pharmacological similarity of the presynaptic inhibitory 5-HT autoreceptor and the 5-HT receptor site defined by [125I]ICYP binding in spinal cord synaptosomes (r = 0.791, P = .0193). Tritium 134-136 5-hydroxytryptamine receptor 1B Rattus norvegicus 36-42 1314960-2 1992 In both rat and human cerebellar membrane preparations saturation studies with [3H]haloperidol (non-specific binding defined by pentazocine, 10 mumolsL-1) demonstrated high affinity saturable specific binding to a homogenous population of binding sites (rat, Bmax 6693 +/- 1242 fmol mg-1 protein, pKD 8.33 +/- 0.08; human, Bmax 2550 +/- 437 fmol mg-1 protein, pKD 8.59 +/- 0.11; mean +/- SEM, n = 3-6). Tritium 80-82 protein kinase D1 Homo sapiens 297-300 1314960-2 1992 In both rat and human cerebellar membrane preparations saturation studies with [3H]haloperidol (non-specific binding defined by pentazocine, 10 mumolsL-1) demonstrated high affinity saturable specific binding to a homogenous population of binding sites (rat, Bmax 6693 +/- 1242 fmol mg-1 protein, pKD 8.33 +/- 0.08; human, Bmax 2550 +/- 437 fmol mg-1 protein, pKD 8.59 +/- 0.11; mean +/- SEM, n = 3-6). Tritium 80-82 protein kinase D1 Homo sapiens 360-363 1346360-2 1992 Somatostatin inhibits HGF-induced (at a dose of 10 ng/mL) or EGF-induced (at a dose of 100 ng/mL) 3H-thymidine incorporation into hepatocytes in a dose-dependent manner (10(-10) to 10(-8) M). Tritium 98-100 hepatocyte growth factor Rattus norvegicus 22-25 1359365-2 1992 The specific activity of the [3H]YM09151-2 binding to transfected (Ltk-RGB) cells is 4-5 fold higher than untransfected (Ltk-) cells. Tritium 30-32 leukocyte tyrosine kinase Mus musculus 67-70 8086501-8 1994 The number of active binding sites on hybrid CTB was determined from: (i) titration with the oligosaccharide moiety of GM1 (oligo-GM1) and monitoring the reversal of the Trp fluorescence quenching by iodide ions and (ii) rapid gel filtration over a superdex HR column of a mixture of hybrid CTB and an excess of 3H-labeled oligo-GM1. Tritium 312-314 phosphate cytidylyltransferase 1B, choline Homo sapiens 45-48 7524992-11 1994 The phosphodiesterase inhibitor, IBMX (10-3 M)significantly raised [3H]-cyclic GMP levels, and forskolin (3 x 10- M) in the presence of IBMX had no significant effect on either basal or SNP-stimulated [3H]-cyclic GMP levels (e.g. in the presence of IBMX: SNP (10-3 M): 660 +/- 90% of basal; SNP + forskolin: 790 +/- 86% of basal, n = 3).5. Tritium 68-70 5'-nucleotidase, cytosolic II Homo sapiens 79-82 7524992-13 1994 The ability of forskolin to potentiate SNP- and ANP-stimulated [3H]-cyclic GMP accumulation may suggest a cyclic AMP-cyclic GMP interaction at the level of the phosphodiesterases.Further, the ability of cyclic AMP to influence cyclic GMP levels may indicate that the two nucleotides, as well as having independent mechanisms to induce smooth muscle relaxation, could produce vasodilatation via a common mechanism. Tritium 64-66 5'-nucleotidase, cytosolic II Homo sapiens 75-78 7524992-13 1994 The ability of forskolin to potentiate SNP- and ANP-stimulated [3H]-cyclic GMP accumulation may suggest a cyclic AMP-cyclic GMP interaction at the level of the phosphodiesterases.Further, the ability of cyclic AMP to influence cyclic GMP levels may indicate that the two nucleotides, as well as having independent mechanisms to induce smooth muscle relaxation, could produce vasodilatation via a common mechanism. Tritium 64-66 5'-nucleotidase, cytosolic II Homo sapiens 124-127 7524992-13 1994 The ability of forskolin to potentiate SNP- and ANP-stimulated [3H]-cyclic GMP accumulation may suggest a cyclic AMP-cyclic GMP interaction at the level of the phosphodiesterases.Further, the ability of cyclic AMP to influence cyclic GMP levels may indicate that the two nucleotides, as well as having independent mechanisms to induce smooth muscle relaxation, could produce vasodilatation via a common mechanism. Tritium 64-66 5'-nucleotidase, cytosolic II Homo sapiens 124-127 7874236-4 1994 In addition, KCl-induced LTP was associated with an increase in 3H-AMPA ([3H]-amino-3-hydroxy-5-methylisoxazole-4-propionate) binding to CA1 synaptic membranes when measured 40 min after high-potassium exposure; however, no changes were detected in binding of an antagonist ([3H]-6-cyano-7-nitroquinoxaline-2,3-dione; 3H-CNQX) to AMPA receptors in slices expressing KCl-induced LTP. Tritium 64-66 carbonic anhydrase 1 Homo sapiens 137-140 7874236-4 1994 In addition, KCl-induced LTP was associated with an increase in 3H-AMPA ([3H]-amino-3-hydroxy-5-methylisoxazole-4-propionate) binding to CA1 synaptic membranes when measured 40 min after high-potassium exposure; however, no changes were detected in binding of an antagonist ([3H]-6-cyano-7-nitroquinoxaline-2,3-dione; 3H-CNQX) to AMPA receptors in slices expressing KCl-induced LTP. Tritium 74-76 carbonic anhydrase 1 Homo sapiens 137-140 1359365-2 1992 The specific activity of the [3H]YM09151-2 binding to transfected (Ltk-RGB) cells is 4-5 fold higher than untransfected (Ltk-) cells. Tritium 30-32 leukocyte tyrosine kinase Mus musculus 121-124 1359365-3 1992 The antibody is able to inhibit the [3H]YM09151-2 binding to the cell membranes from Ltk-RGB cells (Bmax 110.56 +/- 5.26 and 76.20 +/- 5.18 fmoles/mg protein in the presence of preimmune and immune sera, respectively, with no change in the Kd). Tritium 37-39 leukocyte tyrosine kinase Mus musculus 85-88 1662619-4 1991 Three lines of evidence indicate phospholipase A2 activity to be involved in arachidonic acid release: (a) its inhibition by mepacrine, (b) the concomitant generation of lysophosphatidylcholine from [3H]choline-labeled cells and (c) an increase in arachidonic acid release from 14C-labeled phosphatidylcholine in particulate fractions from PMA-treated and bradykinin-treated keratinocytes. Tritium 200-202 phospholipase A2, group IB, pancreas Mus musculus 33-49 8979820-3 1996 Autoradiography of tissue sections exposed to [3H]-8-OH-DPAT (8-hydroxy-dipropylaminotetraline) or to [125I]cyanopindolol plus isoproterenol showed that 5-HT1A and 5-HT1B receptors, respectively, were present in the superficial SC layers. Tritium 47-49 5-hydroxytryptamine receptor 1A Homo sapiens 153-159 1789930-6 1991 It cannot hydrolyse 3H-labelled insoluble elastin and apolipoprotein AII, but did cleave a dinitrophenyl-octapeptide as well as apolipoprotein AI to 25-kDa and 24-kDa fragments formed sequentially. Tritium 20-22 elastin Homo sapiens 42-49 7518822-4 1994 Moreover, the rate of [3H]hyaluronan degradation was highly correlated with the amount of CD44 (r = 0.951, P < 0.0001), as well as with the invasive potential of the cells. Tritium 23-25 CD44 molecule (Indian blood group) Homo sapiens 90-94 8034615-9 1994 By Scatchard analysis, the Kd values for [3H]9-cis-retinoic acid to the GST-hRXR alpha (DEF) peptide and the cleaved DEF peptide were estimated to be 1.8 nM and 5.6 nM, respectively. Tritium 42-44 retinoid X receptor alpha Homo sapiens 76-86 8527441-9 1995 Differentiated HL-60 cells exposed to an RFP antisense oligonucleotide selectively lost 3H-LXA4 binding as well as LXA4-stimulated lipid remodeling that paralleled the loss of mRNA for LXA4R. Tritium 88-90 formyl peptide receptor 2 Homo sapiens 41-44 8013350-12 1994 Authentic human POMC-(1-76), at a concentration of 10 nM provoked a similar stimulation of the [3H]T-labeled lactotroph number without affecting other cell types. Tritium 96-98 proopiomelanocortin Rattus norvegicus 16-20 7969896-5 1994 By means of combined immunohistochemical and autoradiographic techniques it was demonstrated that many neurons and astrocytes which expressed binding sites for [3H]cholecystokinin, [3H]L-365,260 and [125I]bombesin were also immunostained by the monoclonal muscarinic receptor antibody M 35 providing evidence for a co-localization of peptidergic and cholinergic receptors on the membrane of these cells. Tritium 161-163 cholecystokinin Rattus norvegicus 164-179 7801776-4 1994 IC50 and K1 values of l-norepinephrine (NE), dl-propranol (Pro), d-timolol (d-Tim), and l-Tim competed for the [3H]DHA binding sites were 171 +/- 10, 10.3 +/- 4.3, 6.5 +/- 2.1, 0.40 +/- 0.23 nmol.L-1 and 113 +/- 6, 6.3 +/- 2.8, 4.0 +/- 1.3, 0.25 +/- 0.14 nmol.L-1, respectively. Tritium 112-114 translocation induced circling mutation Mus musculus 78-81 7801776-4 1994 IC50 and K1 values of l-norepinephrine (NE), dl-propranol (Pro), d-timolol (d-Tim), and l-Tim competed for the [3H]DHA binding sites were 171 +/- 10, 10.3 +/- 4.3, 6.5 +/- 2.1, 0.40 +/- 0.23 nmol.L-1 and 113 +/- 6, 6.3 +/- 2.8, 4.0 +/- 1.3, 0.25 +/- 0.14 nmol.L-1, respectively. Tritium 112-114 translocation induced circling mutation Mus musculus 90-93 7801776-5 1994 These 4 agents competed for the [3H]DHA binding sites in an order of potency: l-Tim > d-Tim > Pro > NE. Tritium 33-35 translocation induced circling mutation Mus musculus 80-83 7801776-5 1994 These 4 agents competed for the [3H]DHA binding sites in an order of potency: l-Tim > d-Tim > Pro > NE. Tritium 33-35 translocation induced circling mutation Mus musculus 91-94 1786554-3 1991 [3H]IP3 binding was extremely sensitive to ischemic insult, and more than 80% of the binding sites were lost after destruction of CA1 pyramidal cells following 6-min ischemia and three 2-min ischemic insults. Tritium 1-3 carbonic anhydrase 1 Homo sapiens 130-133 1786554-5 1991 [3H]PDBu binding in the CA1 subfield decreased by 1 day after three 2-min ischemic insults and by 4 days after 6-min ischemia, and 40-50% reductions were observed at 1 month. Tritium 1-3 carbonic anhydrase 1 Homo sapiens 24-27 1834802-3 1991 [3H]5-HT competition studies using high-affinity 5-HT1B compounds performed in the presence of 100 nM 8-OH-DPAT (to block 5-HT1A receptors) indicated that approximately 26% of all specific, high-affinity [3H]5-HT binding to spinal cord synaptosomes was to 5-HT1B receptors. Tritium 1-3 5-hydroxytryptamine receptor 1B Rattus norvegicus 49-55 1834802-4 1991 [3H]5-HT competition studies performed in the presence of 100 nM 8-OH-DPAT and 10 nM RU 24969 (to block 5-HT1A and 5-HT1B receptors, respectively) indicated that the remaining 49% of [3H]5-HT binding did not possess the pharmacologic profile previous reported for 5-HT1C, 5-HT1D, 5-HT1E, 5-HT2, or 5-HT3 receptors. Tritium 1-3 5-hydroxytryptamine receptor 1B Rattus norvegicus 115-121 1655252-8 1991 Over 24 h, at 25 nM, [3H]folic acid uptake in L1210-B73 cells was found to proceed for more than 98% via the mFBP. Tritium 22-24 far upstream element (FUSE) binding protein 1 Mus musculus 109-113 1655252-10 1991 With respect to antifolates, the mFBP in L1210-B73 cells contributed for less than 30% in the uptake of [3H]MTX but was the predominant route (92%) in the uptake of [3H]ICI-198,583. Tritium 105-107 far upstream element (FUSE) binding protein 1 Mus musculus 33-37 1655252-10 1991 With respect to antifolates, the mFBP in L1210-B73 cells contributed for less than 30% in the uptake of [3H]MTX but was the predominant route (92%) in the uptake of [3H]ICI-198,583. Tritium 166-168 far upstream element (FUSE) binding protein 1 Mus musculus 33-37 8206989-2 1994 Both 9-cis-retinoic acid (RA) and all-trans-RA (t-RA) compete for [3H]9-cis-RA binding to RA receptors (RAR alpha, beta, and gamma) in nucleosol fractions from transiently transfected COS-1 cells with IC50 values of approximately 12 and 5 nM, respectively. Tritium 67-69 retinoic acid receptor, alpha Mus musculus 104-113 8527441-11 1995 Treatment of human neutrophils with antisera raised against a peptide in the LXA4R third extracellular domain also resulted in selective abrogation of 3H-LXA4 specific binding with polymorphonuclear leukocytes (PMN) without blocking 3H-FMLP binding. Tritium 151-153 formyl peptide receptor 2 Homo sapiens 77-82 8206989-3 1994 Curiously, 9-cis-RA competes for [3H]t-RA binding to mouse RAR alpha, beta, and gamma with IC50 values of 31, 8, and 60 nM, respectively, while t-RA itself does not exhibit such differential competition (IC50 values for RARs, 5 nM). Tritium 34-36 retinoic acid receptor, alpha Mus musculus 59-68 8188767-11 1994 The anti-IGF-I receptor antibody alpha IR3 blocked the IGF-I and IGF-II stimulated increase of 3H-thymidine uptake. Tritium 95-97 insulin like growth factor 1 receptor Homo sapiens 9-23 8788437-3 1995 Specific binding of [3H][beta-Ala8]neurokinin A-(4-10) was displaced only by NK2, but not by NK1 or NK3, tachykinin receptor agonists and antagonists. Tritium 21-23 tachykinin receptor 1 Homo sapiens 93-96 1839140-4 1991 The maximal reduction of [3H]NPA binding (35%) was obtained within 5 min from the addition of neurotensin. Tritium 26-28 neurotensin Rattus norvegicus 94-105 8618850-4 1995 The [3H]palmitate labeling of GluR6 was eliminated by treatment with hydroxylamine, indicating that the labeling was due to palmitoylation at a cysteine residue via a thioester bond. Tritium 5-7 glutamate ionotropic receptor kainate type subunit 2 Homo sapiens 30-35 7936128-0 1994 Comparative 3H-CCK-8S binding studies on CCKB-type receptors in guinea-pig cortex and continuous cell lines. Tritium 12-14 cholecystokinin Cavia porcellus 15-18 7936128-2 1994 The rank order of potency of a variety of CCK agonists and antagonists in inhibiting specific [3H]CCK-8S binding was highly correlated for the 4 CCKB receptor models as demonstrated by a computer-assisted statistical analysis. Tritium 95-97 cholecystokinin Cavia porcellus 42-45 1930143-11 1991 Biosynthesis of the mucin oligomers was studied by labelling of stomach explants in vitro with [35S]methionine, [3H]galactose or [35S]sulphate and subsequent immunoprecipitation of the mucin with a specific antiserum. Tritium 113-115 solute carrier family 13 member 2 Rattus norvegicus 20-25 7595540-4 1995 We examined the in vitro processing of [3H]leucine-labeled or unlabeled proTRH by partially purified recombinant PC1. Tritium 40-42 proprotein convertase subtilisin/kexin type 1 Rattus norvegicus 113-116 1680165-4 1991 SAA-stimulated release of [3H]GABA was shown to comprise two distinct components, calcium-dependent and calcium-independent, which occur after activation of N-methyl-D-aspartate (NMDA) and non-NMDA receptors. Tritium 27-29 serum amyloid A cluster Mus musculus 0-3 7936128-2 1994 The rank order of potency of a variety of CCK agonists and antagonists in inhibiting specific [3H]CCK-8S binding was highly correlated for the 4 CCKB receptor models as demonstrated by a computer-assisted statistical analysis. Tritium 95-97 cholecystokinin Cavia porcellus 98-101 7592963-4 1995 Transfection of COS.M6 cells with cardiac alpha 1C-a (alpha 1) led to the appearance of dihydropyridine ([3H]PN200-110) binding which was increased by coexpression of cardiac beta 2a (beta), alpha 2 delta a (alpha 2), and the skeletal muscle gamma. Tritium 106-108 MGC75582, possible similarity to act2 S homeolog Xenopus laevis 191-198 8197121-2 1994 WIN 64338 inhibits [3H]-bradykinin binding to the bradykinin B2 receptor on human IMR-90 cells with a binding inhibition constant (Ki) of 64 +/- 8 nM and demonstrates competitive inhibition of bradykinin-stimulated 45Ca2+ efflux from IMR-90 cells (pA2 = 7.1). Tritium 20-22 B2 bradykinin receptor Oryctolagus cuniculus 50-72 7913398-2 1994 Neurotensin (10 nM) increased the KD and the Bmax values of [3H]7-OH-DPAT binding, and these effects persisted also following N-ethylmaleimide pretreatment. Tritium 61-63 neurotensin Rattus norvegicus 0-11 8149483-2 1994 Application of TPA to mouse skin decreased the binding of [3H]RA to RAR from mouse epidermal nuclear extracts. Tritium 59-61 retinoic acid receptor, alpha Mus musculus 68-71 8149483-3 1994 In this experiment, 20 nmol of TPA was applied to mouse skin and 3.5 h later binding of [3H]RA to RAR was analyzed by chromatography on a size-exclusion column. Tritium 89-91 retinoic acid receptor, alpha Mus musculus 98-101 8149483-4 1994 TPA treatment resulted in an approximately 67% decrease in the specific binding of [3H]RA to RAR. Tritium 84-86 retinoic acid receptor, alpha Mus musculus 93-96 7512501-3 1994 DAF found in the HeLa cell culture supernatants contained both [3H]ethanolamine and [3H]inositol, but not [3H]palmitic acid, whereas the soluble heparan sulfate proteoglycan present in bone marrow stromal cell culture supernatants contained [3H]ethanolamine. Tritium 64-66 CD55 molecule (Cromer blood group) Homo sapiens 0-3 8070538-4 1994 Collagen synthesis, assessed by the [3H]proline incorporation method, was significantly increased in the right ventricle of 3-month-old Tsk mice. Tritium 37-39 fibrillin 1 Mus musculus 136-139 1917960-5 1991 The addition of ET-1 and ET-2 at 1-50 nM concentrations caused human melanocytes to significantly stimulate DNA [( 3H]thymidine incorporation) and melanin synthesis (3H2O release and [14C] thiouracil incorporation). Tritium 115-117 endothelin 2 Homo sapiens 25-29 1654114-3 1991 This process is mediated through the activation of phospholipase C. Bradykinin at the same concentration also stimulated the production of PGE2 and caused a release of 3H radioactivity from the cells prelabeled with [3H]arachidonic acid, probably via the activation of phospholipase A2. Tritium 168-170 phospholipase A2, group IB, pancreas Mus musculus 269-285 1892900-6 1991 Furthermore, immunoprecipitation and protein A-Sepharose adsorption analysis revealed that in the calf uterine cytosol, the [3H]R5020-receptor complexes were recognized by anti-progesterone receptor monoclonal antibody PR6. Tritium 125-127 progesterone receptor Bos taurus 177-198 7592963-4 1995 Transfection of COS.M6 cells with cardiac alpha 1C-a (alpha 1) led to the appearance of dihydropyridine ([3H]PN200-110) binding which was increased by coexpression of cardiac beta 2a (beta), alpha 2 delta a (alpha 2), and the skeletal muscle gamma. Tritium 106-108 MGC75582, possible similarity to act2 S homeolog Xenopus laevis 208-215 1908878-4 1991 IFN-gamma inhibited [3H]TdR incorporation elicited by CSF-1, and to a much lesser extent, GM-CSF. Tritium 21-23 colony stimulating factor 1 (macrophage) Mus musculus 54-59 8045673-9 1994 Scatchard analysis of [3H]PAF binding to elicited BALB/c macrophages revealed the existence of high affinity receptors for PAF. Tritium 23-25 patchy fur Mus musculus 26-29 7488643-6 1995 However, when ancrod was present in plasma, it caused an increase of [3H]IP1 of HUVECs preloaded with [3H]myoinositol. Tritium 70-72 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 73-76 8045673-9 1994 Scatchard analysis of [3H]PAF binding to elicited BALB/c macrophages revealed the existence of high affinity receptors for PAF. Tritium 23-25 patchy fur Mus musculus 123-126 1747759-3 1991 Melatonin inhibited calmodulin-dependent phosphodiesterase and when either melatonin or [3H]melatonin was preincubated with calmodulin and separated by electrophoresis, comigration of calmodulin with the radioactivity as well as modification of the Ca2+ calmodulin shift were observed. Tritium 89-91 calmodulin 2 Mus musculus 124-134 8550082-0 1995 Monocyte chemotactic protein-1 provokes mast cell aggregation and [3H]5HT release. Tritium 67-69 C-C motif chemokine ligand 2 Rattus norvegicus 0-30 1747759-3 1991 Melatonin inhibited calmodulin-dependent phosphodiesterase and when either melatonin or [3H]melatonin was preincubated with calmodulin and separated by electrophoresis, comigration of calmodulin with the radioactivity as well as modification of the Ca2+ calmodulin shift were observed. Tritium 89-91 calmodulin 2 Mus musculus 124-134 1747759-3 1991 Melatonin inhibited calmodulin-dependent phosphodiesterase and when either melatonin or [3H]melatonin was preincubated with calmodulin and separated by electrophoresis, comigration of calmodulin with the radioactivity as well as modification of the Ca2+ calmodulin shift were observed. Tritium 89-91 calmodulin 2 Mus musculus 124-134 7512985-11 1994 The extent and kinetics of this effect of LPS on cPLA2 parallel the priming of [3H]AA release, both depending on LPS concentration either with or without LBP. Tritium 80-82 phospholipase A2 group IVA Homo sapiens 49-54 8014598-4 1994 LHRH induced the release of [3H]arachidonic acid ([3H]AA) from [3H]AA-prelabelled anterior pituitary tissue from pro-oestrous rats; a response which was sensitive to inhibitors of PLA2, of protein kinase C (PKC) and of protein synthesis. Tritium 29-31 protein kinase C, gamma Rattus norvegicus 189-205 7475275-3 1995 GM-CSF sensitivity was determined by in vitro incubation of blasts for 48 h with or without GM-CSF (100 U/ml) followed by an additional 4 h concurrent exposure to GM-CSF and 3H-TdR (0.5 microCi/ml). Tritium 174-176 colony stimulating factor 2 Homo sapiens 0-6 8014598-4 1994 LHRH induced the release of [3H]arachidonic acid ([3H]AA) from [3H]AA-prelabelled anterior pituitary tissue from pro-oestrous rats; a response which was sensitive to inhibitors of PLA2, of protein kinase C (PKC) and of protein synthesis. Tritium 29-31 protein kinase C, gamma Rattus norvegicus 207-210 8014598-4 1994 LHRH induced the release of [3H]arachidonic acid ([3H]AA) from [3H]AA-prelabelled anterior pituitary tissue from pro-oestrous rats; a response which was sensitive to inhibitors of PLA2, of protein kinase C (PKC) and of protein synthesis. Tritium 51-53 protein kinase C, gamma Rattus norvegicus 189-205 1860838-6 1991 A protein kinase C activator phorbol 12,13-dibutyrate induces an extracellular Ca(2+)-dependent, slowly developing sustained increase in [3H]AA release, and markedly potentiates both phases of bombesin-induced [3H]AA release. Tritium 211-213 gastrin releasing peptide Homo sapiens 193-201 8014598-4 1994 LHRH induced the release of [3H]arachidonic acid ([3H]AA) from [3H]AA-prelabelled anterior pituitary tissue from pro-oestrous rats; a response which was sensitive to inhibitors of PLA2, of protein kinase C (PKC) and of protein synthesis. Tritium 51-53 protein kinase C, gamma Rattus norvegicus 207-210 7475275-6 1995 Blast cells of 37 from 55 analyzed patients were found to be sensitive to GM-CSF stimulation as defined by an increase in 3H-TdR incorporation > or = 1.5-fold over control values after the 48 h GM-CSF exposure. Tritium 122-124 colony stimulating factor 2 Homo sapiens 74-80 8014598-5 1994 Activation of PKC also resulted in [3H]AA release which was inhibited with exactly the same pharmacological profile as the response to LHRH. Tritium 36-38 protein kinase C, gamma Rattus norvegicus 14-17 1834309-7 1991 Areas almost exclusively containing 5-HT1B or 5-HT1D sites, such as the ventral pallidum, globus pallidus and substantia nigra, expressed markedly more [3H]5-HT binding in rat as compared to guinea pig, while the opposite occurred in claustrum, dorsal endopiriform nucleus, lateral geniculate nucleus, and superficial grey layer of the superior colliculus. Tritium 153-155 5-hydroxytryptamine receptor 1B Rattus norvegicus 36-42 1834309-9 1991 The distribution of [3H]eltoprazine binding sites showed a good correlation with that of the 5-HT1B sites in rat (r = 0.89), and with that of the 5-HT1A sites in guinea pig (r = 0.97). Tritium 21-23 5-hydroxytryptamine receptor 1B Rattus norvegicus 93-99 1907914-1 1991 The elongation factor 1 alpha (aEF-1 alpha) was purified to homogeneity from the thermoacidophilic archaebacterium Sulfolobus solfataricus by chromatographic procedures utilising DEAE-Sepharose, hydroxyapatite and FPLC on Mono S. The purified protein binds [3H]GDP at a 1:1 molar ratio and it is essential for poly(Phe) synthesis in vitro; it also binds GTP but not ATP. Tritium 258-260 Hsp20/alpha crystallin family protein Saccharolobus solfataricus 4-29 8043973-3 1994 AM were obtained by bronchoalveolar lavage from patients with emphysema and from patients with non obstructive chronic pulmonary diseases (non-COPD) and cultured under serum-free conditions in direct contact with 3H-labeled elastin particles. Tritium 213-215 elastin Homo sapiens 224-231 8043973-4 1994 Elastinolytic activity was calculated from the released radioactivity in culture supernatants and expressed as micrograms of 3H-elastin degraded x 10(-5) AM x 72 h-1. Tritium 125-127 elastin Homo sapiens 128-135 8074989-2 1994 Ornithine decarboxylase (ODC), a key enzyme in polyamine biosynthesis, and thus in tissue growth and development, has been localized in mouse dental tissues, in vivo as well as in vitro by light and electron microscopic autoradiography with radiolabeled alpha-difluoromethylornithine ([3H]DFMO). Tritium 285-289 ornithine decarboxylase, structural 1 Mus musculus 0-23 8637611-4 1995 In Alzheimer"s disease, compared with age- and post mortem interval-matched controls, there was a decrease in [3H]phosphatidylinositol hydrolysis stimulated by G-proteins and by several receptor agonists, but the levels of Galphaq and most of the phospholipase C and protein kinase C isoforms were unaffected. Tritium 111-113 G protein subunit alpha q Homo sapiens 223-230 8074989-2 1994 Ornithine decarboxylase (ODC), a key enzyme in polyamine biosynthesis, and thus in tissue growth and development, has been localized in mouse dental tissues, in vivo as well as in vitro by light and electron microscopic autoradiography with radiolabeled alpha-difluoromethylornithine ([3H]DFMO). Tritium 285-289 ornithine decarboxylase, structural 1 Mus musculus 25-28 7984293-1 1994 Specific binding for the serotonin 5-HT4 receptor (5-HT4R) radioligand [3H]GR 113808 was identified in pig caudate nucleus and characterized by serotonin subtype selective drugs. Tritium 72-74 5-hydroxytryptamine receptor 4 Sus scrofa 51-57 2071940-4 1991 Although maximal uptake of [3H]retinol added directly to the culture medium occurred at 3 h, the uptake of [3H]retinol from RBP was linear with time for at least 72 h. By 57 h, cell-associated [3H]retinol was the same whether it was added directly to the culture medium or bound to RBP. Tritium 108-110 retinol binding protein 4 Homo sapiens 124-127 2071940-4 1991 Although maximal uptake of [3H]retinol added directly to the culture medium occurred at 3 h, the uptake of [3H]retinol from RBP was linear with time for at least 72 h. By 57 h, cell-associated [3H]retinol was the same whether it was added directly to the culture medium or bound to RBP. Tritium 108-110 retinol binding protein 4 Homo sapiens 124-127 1650838-2 1991 The present study utilized the photoaffinity ligand [3H]PK14105 to identify the protein subunit in rat aortic and other smooth muscle types to which high affinity ligands for the PBR bind. Tritium 53-55 translocator protein Rattus norvegicus 179-182 8584761-5 1995 administration of antisense oligodeoxynucleotide to the cloned delta opioid receptor selectively decreased [3H][D-Ala2,D-Leu5]enkephalin binding to the delta ncx site, not the delta cx-2 site. Tritium 108-111 tripartite motif containing 13 Homo sapiens 121-125 1721559-11 1991 Among these proteins, [3H]-kE was found to bind exclusively to a 65 kDa protein that could be eluted selectively from elastin fibres with a neutral buffer containing 100 mM lactose. Tritium 23-25 elastin Homo sapiens 118-125 8127890-7 1994 Plasma from normal and apoA-I-deficient mice, but not from apoE-deficient mice, released [3H]cholesterol from fibroblasts into a gamma-migrating lipoprotein. Tritium 90-92 apolipoprotein A-I Mus musculus 23-29 8180805-4 1994 The decreases in [3H]GBR-12935 binding to DA uptake sites in this study indicate a marked degeneration of DA neurites in the putamen in PD and also in DAT. Tritium 18-20 solute carrier family 6 member 3 Homo sapiens 151-154 8672608-5 1995 One day after the last of 12 ECSs, a decrease in [3H]IP3 binding (21%) was found within the CA1 region of the hippocampus and the piriform cortex (39%). Tritium 50-52 carbonic anhydrase 1 Rattus norvegicus 92-95 8117097-7 1994 In particular, [3H]TCDD was released faster from the liganded induced protein than from the constitutive Ah receptor. Tritium 16-18 aryl hydrocarbon receptor Rattus norvegicus 105-116 8313359-10 1994 Bombesin was also shown to stimulate the DNA synthesis in CFPAC-1 cells in vitro as based on [3H]thymidine incorporation assay. Tritium 94-96 gastrin releasing peptide Homo sapiens 0-8 2070498-4 1991 The MGMT activity was assayed by measuring the 3H radioactivity transferred from the substrate DNA containing [methyl-3H]-labeled O6-methylguanine to an acid-insoluble protein fraction. Tritium 47-49 O-6-methylguanine-DNA methyltransferase Homo sapiens 4-8 1920900-2 1991 When acini were incubated with [3H] choline in the presence of 1 nM CCK-octapeptide (CCK8) for 60 min, the incorporations of [3H] choline to both water soluble choline metabolites and PC in acini were reduced by CCK8 to 74% and 41% of control, respectively. Tritium 32-34 cholecystokinin Rattus norvegicus 68-71 1920900-2 1991 When acini were incubated with [3H] choline in the presence of 1 nM CCK-octapeptide (CCK8) for 60 min, the incorporations of [3H] choline to both water soluble choline metabolites and PC in acini were reduced by CCK8 to 74% and 41% of control, respectively. Tritium 126-128 cholecystokinin Rattus norvegicus 68-71 8313359-14 1994 Concomitant addition of RC-3095 (1 microM) and bombesin (1-100 nM) to the cultures induced a significant reduction in the uptake of [3H]thymidine by the cells compared to the values obtained with bombesin alone. Tritium 133-135 gastrin releasing peptide Homo sapiens 47-55 8706205-5 1995 [3H]-diazepam radioligand binding assays demonstrated that the number of receptors in the septic rats was increased in the forebrain (CLP rats; 2.37 +/- 0.04 pmol x mg(-1) protein, control rats; 1.45 +/- 0.02 pmol x mg(-1) protein, sham-operated rats; 1.49 +/- 0.03 pmol x mg(-1) protein), cerebellum (CLP rats; 1.55 +/- 0.05 pmol x mg(-1) protein, control rats; 1.05 +/- 0.02 pmol x mg(-1) protein, sham-operated rats: 1.09 +/- 0.02 pmol x mg(-1) protein) and brain stem (CLP rats; 1.21 +/- 0.04 pmol x mg(-1) protein, control rats; 0.61 +/- 0.02 pmol x mg(-1) protein, sham-operated rats; 0.63 +/- 0.02 pmol x mg(-1) protein) compared with the control and sham-operated rats (P< 0.05). Tritium 1-3 coactosin-like F-actin binding protein 1 Rattus norvegicus 134-137 8299588-4 1994 Insulin showed a two-phase dose-response curve, increasing [3H]thymidine uptake by 34 +/- 9% at 10 ng/ml and by 63 +/- 13% at 10 micrograms/ml. Tritium 60-62 LOC105613195 Ovis aries 0-7 2059652-7 1991 Furthermore, Glut 1 was photoaffinity-labelled with [3H]cytochalasin B and subjected to isoelectric focusing in one dimension. Tritium 53-55 solute carrier family 2 member 1 Homo sapiens 13-19 8845300-9 1995 The addition of PRL, IL-2, and TPA significantly stimulated[3H] thymidine incorporation, while only the polypeptide growth factors augmented cell density. Tritium 60-62 interleukin 2 Rattus norvegicus 21-25 1715876-7 1991 Treatment of serum-starved monolayers with substance P, bombesin or complete growth medium containing 10% fetal calf serum resulted in increased [3H]thymidine incorporation. Tritium 146-148 gastrin releasing peptide Homo sapiens 56-64 2033241-4 1991 The 3H-labeled probe was specific for mRNA transcripts of mGK-6, the renal kallikrein gene, which is also expressed in salivary glands. Tritium 4-6 kallikrein 1 Mus musculus 58-63 2033241-6 1991 Resolution obtained with this 3H-labeled probe showed mGK-6 mRNA in striated duct cells to be located on RER and in the nucleus and perinuclear area of the cell. Tritium 30-32 kallikrein 1 Mus musculus 54-59 1904963-4 1991 Incubation of cultured neurons labeled with [3H]ACh in the presence of NPY (10(-14)-10(-6) M) significantly inhibited basal ACh release (83 +/- 16 to 58 +/- 11% of control). Tritium 45-47 pro-neuropeptide Y Cavia porcellus 71-74 8294930-3 1994 An 18-kDa polypeptide was specifically photolabelled in cerebellar astrocytes by [3H]PK 14105, a photolabel for the peripheral-type benzodiazepine receptor. Tritium 82-84 translocator protein Rattus norvegicus 116-155 7904573-4 1994 Incubation of 3H- or 35S-labelled cpn10 with mitochondria confirms these conclusions and shows that cpn10 is imported into mitochondria in an energy-dependent process which is inhibited by the presence of 2,4-dinitrophenol. Tritium 14-16 heat shock protein family E (Hsp10) member 1 Rattus norvegicus 100-105 8082044-3 1994 In malnourished rats, 5-HT1A receptors assayed with [3H]8-OH-DPAT were decreased by 20% in CA3. Tritium 53-55 carbonic anhydrase 3 Rattus norvegicus 91-94 8287615-7 1994 The [3H]gelatin degradation assay required a combination of > 50 ng/ml of MMP-2 and MMP-9 for detection. Tritium 5-7 matrix metallopeptidase 9 Homo sapiens 87-92 7813651-0 1994 Reduction of GABAA receptor binding of [3H]muscimol in the barrel field of mice after peripheral denervation: transient and long-lasting effects. Tritium 40-42 gamma-aminobutyric acid (GABA) A receptor, subunit gamma 1 Mus musculus 13-18 1870448-10 1991 Synthesis and secretion of PG-S1, -S2 and fibromodulin wa studied by labeling with [3H]-leucine and [35S]-sulfate. Tritium 84-86 fibromodulin Homo sapiens 42-54 7556432-3 1995 EGF at 1 ng/ml increased the [3H]thymidine labeling index from approximately 1% (control value) to 60% and stimulated fivefold the number of cells invading the collagen matrix. Tritium 30-32 epidermal growth factor Homo sapiens 0-3 1922921-1 1991 N-Methyl-D-aspartate (NMDA)-induced translocation of protein kinase C (PKC) from cytosol to membrane fractions was examined by the methods of [3H]phorbol 12,13-dibutyrate binding and western blotting in rat hippocampal slices. Tritium 143-145 protein kinase C, gamma Rattus norvegicus 53-69 7813651-1 1994 The effect of peripheral sensory deprivation upon GABAA receptor binding of [3H]muscimol was investigated in the barrel cortex--cortical representation of mystacial vibrissae of mice--by means of in vitro quantitative autoradiography. Tritium 77-79 gamma-aminobutyric acid (GABA) A receptor, subunit gamma 1 Mus musculus 50-55 1922921-1 1991 N-Methyl-D-aspartate (NMDA)-induced translocation of protein kinase C (PKC) from cytosol to membrane fractions was examined by the methods of [3H]phorbol 12,13-dibutyrate binding and western blotting in rat hippocampal slices. Tritium 143-145 protein kinase C, gamma Rattus norvegicus 71-74 7556432-5 1995 EGF-stimulated [3H]thymidine incorporation was reduced by TGF-beta 1 at concentrations above 0.1 ng/ml. Tritium 16-18 epidermal growth factor Homo sapiens 0-3 8282065-4 1994 Expression of GM-CSF mRNA was preceded by IL-1 plus TNF-alpha induced arachidonate release (assayed using the 3H-derivative). Tritium 110-112 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 14-20 1654804-7 1991 Furthermore, when these cells were labeled in alkylacyl-GPC by incubation with [3H]alkyllyso-GPC and then stimulated, [3H]alkylacyl-glycerol ([3H]alkylacyl-Gro) is produced with a time-course similar to that of [32P]PO4 formation and coincident with the decline in alkylacyl-GP accumulation. Tritium 80-82 glycophorin C (Gerbich blood group) Homo sapiens 56-59 7556432-8 1995 Moreover, cell migration was still fourfold over control values in cultures exposed to 1.0 ng/ml EGF and 1.0 ng/ml TGF-beta 1, despite a strongly inhibited [3H]thymidine labeling. Tritium 157-159 epidermal growth factor Homo sapiens 97-100 1654804-7 1991 Furthermore, when these cells were labeled in alkylacyl-GPC by incubation with [3H]alkyllyso-GPC and then stimulated, [3H]alkylacyl-glycerol ([3H]alkylacyl-Gro) is produced with a time-course similar to that of [32P]PO4 formation and coincident with the decline in alkylacyl-GP accumulation. Tritium 80-82 glycophorin C (Gerbich blood group) Homo sapiens 93-96 7913088-2 1994 In order to measure the proliferative activity, colonic epithelium was immunostained for cyclin proliferating cell nuclear antigen (PCNA/cyclin), which appears from the Gl to the S phase of the cell cycle, and compared with labeling obtained by [3H]-thymidine radioautography. Tritium 246-248 proliferating cell nuclear antigen Mus musculus 89-95 1654804-7 1991 Furthermore, when these cells were labeled in alkylacyl-GPC by incubation with [3H]alkyllyso-GPC and then stimulated, [3H]alkylacyl-glycerol ([3H]alkylacyl-Gro) is produced with a time-course similar to that of [32P]PO4 formation and coincident with the decline in alkylacyl-GP accumulation. Tritium 119-121 glycophorin C (Gerbich blood group) Homo sapiens 56-59 7556432-11 1995 In conclusion, TGF-beta 1 modulates the response of porcine thyrocytes to EGF in collagen gel cultures by promoting cell migration along with inhibition of [3H]thymidine incorporation. Tritium 157-159 epidermal growth factor Homo sapiens 74-77 1654804-7 1991 Furthermore, when these cells were labeled in alkylacyl-GPC by incubation with [3H]alkyllyso-GPC and then stimulated, [3H]alkylacyl-glycerol ([3H]alkylacyl-Gro) is produced with a time-course similar to that of [32P]PO4 formation and coincident with the decline in alkylacyl-GP accumulation. Tritium 119-121 glycophorin C (Gerbich blood group) Homo sapiens 56-59 8653397-4 1995 Neurotoxic concentrations of PLA2 stimulated neuronal release of [3H]AA. Tritium 66-68 phospholipase A2 group IB Rattus norvegicus 29-33 1654804-9 1991 Upon stimulation with fMLP or C5a, U937 cells labeled in diacyl-sn-glycero-3-phosphocholine (diacyl-GPC) by incubation with [3H]acyllyso-GPC generate [3H]diacyl-GP, [3H]diacyl-GPEt, and [3H]diacyl-Gro with kinetics similar to those for the generation of the [3H]alkyl products. Tritium 125-127 glycophorin C (Gerbich blood group) Homo sapiens 100-103 8263044-0 1994 Increased densities of binding sites for the "peripheral-type" benzodiazepine receptor ligand [3H]PK11195 in vulnerable regions of the rat brain in thiamine deficiency encephalopathy. Tritium 95-97 translocator protein Rattus norvegicus 45-86 1649243-1 1991 The influence of extracellular fatty acids on the uptake and esterification of [3H]retinol bound to human retinol-binding protein (RBP), to RBP-transthyretin (TTR), or in dispersed form by the human hepatoma, HepG2, and human mammary epithelial carcinoma, MCF-7, cell lines was studied. Tritium 80-82 retinol binding protein 4 Homo sapiens 106-129 7665628-6 1995 Purified ps20 induced a linear and saturable inhibition of [3H]thymidine incorporation in PC-3 prostatic carcinoma cells (half-maximal activity at 2.6 nM), inhibited cell proliferation (increased population doubling time from 19.8 to 25.8 h), and induced a 210% stimulation in the synthesis of secreted proteins. Tritium 60-62 WAP four-disulfide core domain 1 Rattus norvegicus 9-13 1649243-1 1991 The influence of extracellular fatty acids on the uptake and esterification of [3H]retinol bound to human retinol-binding protein (RBP), to RBP-transthyretin (TTR), or in dispersed form by the human hepatoma, HepG2, and human mammary epithelial carcinoma, MCF-7, cell lines was studied. Tritium 80-82 retinol binding protein 4 Homo sapiens 131-134 1649243-4 1991 Comparing equal concentrations of the [3H]retinol donors, cell uptake and esterification was greatest from the dispersed form and least from that bound to RBP-TTR. Tritium 39-41 retinol binding protein 4 Homo sapiens 155-158 1649243-4 1991 Comparing equal concentrations of the [3H]retinol donors, cell uptake and esterification was greatest from the dispersed form and least from that bound to RBP-TTR. Tritium 39-41 transthyretin Homo sapiens 159-162 1649243-9 1991 These effects on [3H]retinol uptake and esterification were dose-dependent as the oleate to albumin ratio was varied from 0.5 to 3.0 and were observed across a physiological concentration range of RBP-3H-retinol. Tritium 18-20 retinol binding protein 4 Homo sapiens 197-200 1850424-9 1991 On a molar basis the recombinant 92-kDa type IV collagenase was approximately 30% as active as human leukocyte elastase in solubilizing 3H-labeled elastin. Tritium 136-138 elastin Homo sapiens 147-154 1882086-8 1991 [3H]HACBO-Gly binds also to a limited number of peripheral tissues in which the presence of NEP was yet unknown (bones, parts of adipose tissues. Tritium 1-3 membrane metallo-endopeptidase Rattus norvegicus 92-95 8121239-4 1994 [3H] Ro 5-4864, an agonist, showed a low affinity (KD = 188.38 +/- 16.46 nM) for human peripheral-type benzodiazepine receptor subtype. Tritium 1-3 translocator protein Homo sapiens 87-126 8302271-0 1994 Pharmacological heterogeneity of the cloned and native human dopamine transporter: disassociation of [3H]WIN 35,428 and [3H]GBR 12,935 binding. Tritium 102-104 solute carrier family 6 member 3 Homo sapiens 61-81 8302271-0 1994 Pharmacological heterogeneity of the cloned and native human dopamine transporter: disassociation of [3H]WIN 35,428 and [3H]GBR 12,935 binding. Tritium 121-123 solute carrier family 6 member 3 Homo sapiens 61-81 8302271-4 1994 [3H]DA uptake was inhibited in a concentration-dependent and uniphasic manner by dopaminergic agents with an appropriate rank order of potency for the DA transporter. Tritium 1-3 solute carrier family 6 member 3 Homo sapiens 151-165 8302271-10 1994 Taken together, these data suggest that 1) [3H]WIN 35,428 recognizes two sites of the DA transporter, of which only one appears to represent the functional state of the protein, and 2) [3H]WIN 35,428 and [3H]GBR 12,935 do not appear to bind the same functional form/state of the DA transporter. Tritium 44-46 solute carrier family 6 member 3 Homo sapiens 86-100 8302271-10 1994 Taken together, these data suggest that 1) [3H]WIN 35,428 recognizes two sites of the DA transporter, of which only one appears to represent the functional state of the protein, and 2) [3H]WIN 35,428 and [3H]GBR 12,935 do not appear to bind the same functional form/state of the DA transporter. Tritium 44-46 solute carrier family 6 member 3 Homo sapiens 279-293 8139704-9 1994 [3H]R-PIA detects more sites than [3H]CCPA (22.8 +/- 4.0 and 8.3 +/- 1.3 fmol/mg protein, respectively; GTP-free conditions). Tritium 1-3 ribose 5-phosphate isomerase A Gallus gallus 4-9 7937350-3 1994 Results showed that [3H]NE release evoked by the omission of Na+ was decreased by 10 nM ANF. Tritium 21-23 natriuretic peptide A Rattus norvegicus 88-91 7643088-4 1995 Two types of superoxide scavengers, Cu2+/Zn2+ superoxide dismutase and ceruloplasmin, significantly reduced the increase in [3H]-GABA release induced by both SNP and SNAP, which assumes that NO requires superoxide to induce [3H]-GABA release from the neurons. Tritium 125-127 ceruloplasmin Homo sapiens 71-84 8262176-4 1993 We report here that recombinant human and bovine PBR produced in yeast, a microorganism devoid of endogenous PBR, can be labelled with [3H]PK11195, but only the human receptor can be labelled with [3H]Ro5-4864. Tritium 136-138 translocator protein Bos taurus 49-52 8262176-4 1993 We report here that recombinant human and bovine PBR produced in yeast, a microorganism devoid of endogenous PBR, can be labelled with [3H]PK11195, but only the human receptor can be labelled with [3H]Ro5-4864. Tritium 198-200 translocator protein Bos taurus 49-52 2018785-3 1991 Glucocorticoid receptor from freshly prepared hepatic cytosol bound [3H]ZK98299 with affinity approximately equal to that of [3H]triamcinolone acetonide. Tritium 69-71 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 0-23 2018785-5 1991 While [3H]ZK98299 binding to glucocorticoid receptor could occur in the presence of iodoacetamide and N-ethylmaleimide (NEM), [3H]triamcinolone acetonide binding capacity was completely abolished following such treatments. Tritium 7-9 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 29-52 2018785-8 1991 The differential effects of iodoacetamide and NEM on the interaction of glucocorticoid receptor with ZK98299 and triamcinolone acetonide, and the faster rate of dissociation of [3H]ZK98299-receptor complexes suggest that treatment with these agents (NEM and iodoacetamide) results in distinct conformational changes in glucocorticoid receptor structure with respect to triamcinolone acetonide and ZK98299 binding. Tritium 178-180 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 319-342 7643088-4 1995 Two types of superoxide scavengers, Cu2+/Zn2+ superoxide dismutase and ceruloplasmin, significantly reduced the increase in [3H]-GABA release induced by both SNP and SNAP, which assumes that NO requires superoxide to induce [3H]-GABA release from the neurons. Tritium 225-227 ceruloplasmin Homo sapiens 71-84 7565624-2 1995 The binding of [3H]CP 55,940 to both CB1 and CB2 was of similar high affinity (2.6 and 3.7 nM, respectively) and saturable. Tritium 16-18 cannabinoid receptor 2 Homo sapiens 45-48 8279569-6 1993 CCK-8 stimulated [3H]thymidine incorporation into DNA in a dose-dependent manner. Tritium 18-20 cholecystokinin Rattus norvegicus 0-3 7642555-9 1995 c-Myc purified from sf9 insect cells was trypsinized, and its GlcNAc moieties were enzymically labeled with [3H]galactose. Tritium 109-111 MYC proto-oncogene, bHLH transcription factor Homo sapiens 0-5 8240296-5 1993 Gastrin cells incorporated [3H]tyrosine, [32P]phosphate and [35S]sulphate into both progastrin and its extreme C-terminal tryptic (nona-) peptide. Tritium 28-30 gastrin Rattus norvegicus 0-7 2004641-2 1991 [3H]Putrescine uptake favored the apical side and its release (in Earle"s balanced salt solution containing 0.1% bovine serum albumin) was six times greater in the apical-to-basolateral than in the basolateral-to-apical direction. Tritium 1-3 albumin Rattus norvegicus 120-133 7653531-4 1995 3H-labeled thymidine proliferation assays showed that cyst fluids and ADPKD epithelial cell-conditioned media also stimulated renal epithelial cell proliferation and contained EGF immunoreactivity (6, 30, and 37 kDa) as detected by Western blots. Tritium 0-2 epidermal growth factor Homo sapiens 176-179 1997179-1 1991 Studies are described addressing the controversial issue of the multiplicity of efflux routes for [3H]MTX in L1210 cells. Tritium 99-101 metaxin 1 Mus musculus 102-105 8223875-5 1993 Stimulation of [3H]PA production upon CD3 cross-linking was 77% lower in permeabilized CD45- cells than in CD45+ cells, consistent with the reduced activity of p59fyn in CD45- cells. Tritium 16-18 protein tyrosine phosphatase receptor type C Homo sapiens 87-91 8223875-5 1993 Stimulation of [3H]PA production upon CD3 cross-linking was 77% lower in permeabilized CD45- cells than in CD45+ cells, consistent with the reduced activity of p59fyn in CD45- cells. Tritium 16-18 protein tyrosine phosphatase receptor type C Homo sapiens 107-111 8223875-5 1993 Stimulation of [3H]PA production upon CD3 cross-linking was 77% lower in permeabilized CD45- cells than in CD45+ cells, consistent with the reduced activity of p59fyn in CD45- cells. Tritium 16-18 protein tyrosine phosphatase receptor type C Homo sapiens 107-111 1997179-3 1991 In ATP-replete cells, the results delineate a probenecid-, bromosulfophthalein-, and verapamil-inhibitable route that accounts for nearly 90% of [3H]MTX efflux. Tritium 146-148 metaxin 1 Mus musculus 149-152 1899867-4 1991 Stimulation with arginine vasopressin, epidermal growth factor, or phorbol myristate acetate increased [3H]thymidine incorporation and mRNA levels of the immediate-early response genes c-fos and Egr-1. Tritium 104-106 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 185-190 1648670-3 1991 The electrically (3 Hz) evoked 3H overflow was inhibited by PYY, NPY and pancreatic polypeptide (decreasing order of potency), but not affected by ACTH1-24, angiotensin II, bradykinin and delta-sleep-inducing peptide. Tritium 31-33 peptide YY Rattus norvegicus 60-63 8223875-7 1993 The CD3-induced increase in total inositol phosphates (InsP) in permeabilized cells was similar to the stimulated production of [3H]PA production in both CD45+ and CD45- cells. Tritium 129-131 protein tyrosine phosphatase receptor type C Homo sapiens 154-158 8398177-4 1993 Tissue culture wells were coated with insoluble 3H-labeled elastin substrate. Tritium 48-50 elastin Homo sapiens 59-66 8398177-7 1993 Elastin degradation was determined as soluble 3H-elastin fragments released into the supernatants. Tritium 46-48 elastin Homo sapiens 0-7 8398177-7 1993 Elastin degradation was determined as soluble 3H-elastin fragments released into the supernatants. Tritium 46-48 elastin Homo sapiens 49-56 7494453-4 1995 In contrast, [3H]nicotine binding to PC 12 cell mutants (A126.1B2 and A123.7), deficient in cAMP-responsive protein kinase A Types I and/or II, was unaffected by nicotine. Tritium 14-16 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 108-124 8221702-4 1993 It appears that the PBR are composed of three subunits: an 18-kDa subunit that binds isoquinoline carboxamide derivatives; a 30-kDa subunit that binds benzodiazepines; and a 32-kDa subunit labeled by the benzodiazepine [3H]AHN 086, the voltage-dependent anion channel. Tritium 220-222 translocator protein Homo sapiens 20-23 8263948-4 1993 The response was transient with maximal immunoreactivity observed 2-3h following treatment, falling to near basal levels in most regions of the heart after 6 h. Although all chambers of the heart contributed to the response, greatest Fos-like immunoreactivity was observed in the left atrium and left ventricle, with intermediate levels found in the septum and right ventricle, and lowest levels in the right atrium. Tritium 68-70 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 234-237 1827514-3 1991 The addition of NMDA in the superfusion medium induced an efflux of [3H]NA from the slices and the presence of NPY and PYY produced an enhancement of the stimulating effect. Tritium 69-71 peptide YY Rattus norvegicus 119-122 1985934-6 1991 A unique cysteine residue at position 145 was identified as the methyl acceptor site by autoradiographic analysis of peptides and sequence analysis of 3H-methylated O6-methylguanine-DNA methyltransferase. Tritium 151-153 O-6-methylguanine-DNA methyltransferase Homo sapiens 165-203 7635938-7 1995 Recombinant human HB-EGF stimulated [3H]-thymidine incorporation in both primary cultures of rabbit proximal tubule cells and NRK 52E normal rat kidney epithelial cells, with potency similar to that of EGF. Tritium 37-39 heparin binding EGF like growth factor Homo sapiens 18-24 2069325-5 1991 In addition, [3H]TdR suicide experiments indicate that TGF-beta 2 blocks the IL-3-induced progression of early progenitors into the S phase of the cell cycle, whereas IL-6 and bFGF potentiate their entry into the mitotic process. Tritium 14-16 transforming growth factor beta 2 Homo sapiens 55-65 7635938-7 1995 Recombinant human HB-EGF stimulated [3H]-thymidine incorporation in both primary cultures of rabbit proximal tubule cells and NRK 52E normal rat kidney epithelial cells, with potency similar to that of EGF. Tritium 37-39 epidermal growth factor Rattus norvegicus 21-24 1913239-2 1991 Quantitative autoradiographic studies on rat and guinea pig brain sections incubated with 2 nM [3H]5-HT showed a preferential affinity of S-CM-GTNH2 for 5-HT1B and 5-HT1D sites. Tritium 96-98 5-hydroxytryptamine receptor 1B Rattus norvegicus 153-159 8523414-4 1995 The binding of [3H]-U69593 was effectively displaced by the selective kappa 1 ligands, U-69593 and U-50488H, but only weakly displaced by Met5-enkephalin-Arg6-Phe7, a selective kappa 2 ligand, which showed only 11 +/- 3% inhibition of [3H]-U69593 binding at the concentration of 1 microM. Tritium 16-18 proenkephalin Rattus norvegicus 143-153 1766257-10 1991 IL-7 increased 3H-TdR incorporation in cells from all five CD7+4-8- acute leukemia and 2 of those with CD5+ or CD1+ leukemia. Tritium 15-17 CD7 molecule Homo sapiens 59-62 8412741-7 1993 Measurement of specific [3H]-phorbol dibutyrate (PDBu) binding suggested translocation of PKC from the cytosol to the membrane fraction after 30 minutes of incubation, only after insulin treatment. Tritium 25-27 protein kinase C, gamma Rattus norvegicus 90-93 7507605-9 1993 EGF, IGF-I (Insulin-like Growth Factor-I), or PDGF (Platelet-derived Growth Factor) alone increased [3H]thymidine incorporation in the cells. Tritium 101-103 epidermal growth factor Gallus gallus 0-3 7507605-10 1993 Adding IGF-I or PDGF simultaneously with EGF enhanced the response of the cells to EGF by increasing [3H]thymidine incorporation. Tritium 102-104 epidermal growth factor Gallus gallus 41-44 7507605-10 1993 Adding IGF-I or PDGF simultaneously with EGF enhanced the response of the cells to EGF by increasing [3H]thymidine incorporation. Tritium 102-104 epidermal growth factor Gallus gallus 83-86 7543631-6 1995 FGF, IGF-1, and EGF were each capable of stimulating [3H]thy uptake on Day 1 of culture (2372 +/- 335 FGF, 2226 +/- 193 IGF-1, 4037 +/- 679 EGF vs 1108 +/- 70 MEM, P < 0.05). Tritium 54-56 epidermal growth factor Homo sapiens 16-19 7507605-11 1993 TGF-beta (Transforming Growth Factor-beta) alone was shown to have an inhibitory effect on [3H]thymidine incorporation, and when TGF-beta was added together with EGF, it attenuated the stimulatory effect of EGF on [3H]thymidine incorporation. Tritium 92-94 epidermal growth factor Gallus gallus 162-165 7507605-11 1993 TGF-beta (Transforming Growth Factor-beta) alone was shown to have an inhibitory effect on [3H]thymidine incorporation, and when TGF-beta was added together with EGF, it attenuated the stimulatory effect of EGF on [3H]thymidine incorporation. Tritium 215-217 epidermal growth factor Gallus gallus 162-165 1677153-3 1991 Two days after lesion of serotonergic afferents to the hippocampus with 5,7-Dihydroxytryptamine [3H]-glutamate binding was significantly decreased in the CA3 region and stratum lacunosum moleculare of the hippocampus, this reduction being reversed by in vitro addition of 10 microM 5-HT. Tritium 97-99 carbonic anhydrase 3 Rattus norvegicus 154-157 1677153-5 1991 Five days after lesion [3H]-glutamate binding increased significantly in the CA3 region of the hippocampus but was not different from sham animals in the other hippocampal layers. Tritium 24-26 carbonic anhydrase 3 Rattus norvegicus 77-80 7543631-7 1995 IGF-1 and EGF also stimulated [3H]thy uptake on Days 3 and 7 of culture. Tritium 31-33 epidermal growth factor Homo sapiens 10-13 1846221-5 1991 Various divalent cations (Mg2+, Mn2+, Ca2+, Ba2+, and Cd2+) were shown to inhibit specific [3H]SR 33557 binding, with Cd2+ being the most potent. Tritium 92-94 Cd2 molecule Rattus norvegicus 54-57 7651369-3 1995 Saturation analysis showed that [3H]Win 55212-2 specific binding to the CB2 receptor was of high affinity, with a Kd of 2.1 +/- 0.2 nM (four experiments), and a high level of expression was attained, with a maximal number of saturable binding sites of 24.1 +/- 4.4 pmol/mg of protein (four experiments). Tritium 33-35 cannabinoid receptor 2 Homo sapiens 72-75 1846221-5 1991 Various divalent cations (Mg2+, Mn2+, Ca2+, Ba2+, and Cd2+) were shown to inhibit specific [3H]SR 33557 binding, with Cd2+ being the most potent. Tritium 92-94 Cd2 molecule Rattus norvegicus 118-121 2266144-5 1990 [3H] Bz2ADP.Pi was also stably trapped on gizzard myosin by forming the 10 S folded conformation of the protein in the presence of [3H]Bz2ATP and Mg2+. Tritium 1-3 myosin, heavy chain 10, non-muscle Gallus gallus 50-56 2266144-5 1990 [3H] Bz2ADP.Pi was also stably trapped on gizzard myosin by forming the 10 S folded conformation of the protein in the presence of [3H]Bz2ATP and Mg2+. Tritium 1-4 myosin, heavy chain 10, non-muscle Gallus gallus 50-56 2266144-7 1990 The 50-kDa and precursor 68-kDa tryptic peptides of the subfragment-1 heavy chain derived from both forms of myosin were found to contain essentially all of the covalently attached [3H]Bz2ADP. Tritium 182-184 myosin, heavy chain 10, non-muscle Gallus gallus 109-115 1963342-4 1990 Administration of either a sedative/ataxic (20 mg/kg) or hypnotic (60 mg/kg) dose of pentobarbital prior to stress blocks the stress-induced decrease of [3H]Ro 5-4864 binding to renal PBR in rat. Tritium 154-156 translocator protein Rattus norvegicus 184-187 8365374-6 1993 After both the [14C]- and [3H]leucine pulses, immunoprecipitable isotope incorporated into ANF appeared in the chase medium within the first 10 min and stabilized to lower levels after 20 min of chase. Tritium 27-29 natriuretic peptide A Rattus norvegicus 91-94 8254183-8 1993 The alpha 2- and beta 2-adrenoceptor characteristics were determined with [3H]-rauwolscine and [125I]-(-1)cyanopindolol, respectively. Tritium 75-78 adrenoceptor beta 2 Homo sapiens 4-36 8281308-3 1993 The spatial and temporal characteristics of PP90 phosphorylation suggested a relationship with the injury-induced proliferation of support cells of the regenerating nerve, i.e. its appearance and increment over time correlated with that of [3H]thymidine incorporation in the nerve. Tritium 241-243 calnexin Canis lupus familiaris 44-48 8229542-7 1993 After 24 h, EGF and TGF-alpha but not insulin-like growth factor I (IGF-I) induced a dose-dependent increase in [3H]thymidine incorporation. Tritium 113-115 protransforming growth factor alpha Oryctolagus cuniculus 20-29 8229542-8 1993 Ten nanomolar EGF or TGF-alpha increased [3H]thymidine incorporation more than sixfold over control. Tritium 42-44 protransforming growth factor alpha Oryctolagus cuniculus 21-30 2249987-8 1990 Similar to other known GPI-linked proteins, uromodulin/THP contains a stretch of 16 hydrophobic amino acids at its extreme carboxyl terminus which could function as a GPI addition signal and was shown to label with [3H]ethanolamine. Tritium 216-218 uromodulin Homo sapiens 55-58 8394992-6 1993 [3H]NKA bound in a protein-dependent, saturable (Bmax = 820 +/- 167 fmol/mg of protein), and highly specific (88 +/- 5%) manner to hNK-2R, but not to membranes from cells infected with wild-type virus (14 +/- 8%, 7 +/- 10 fmol/mg of protein). Tritium 1-3 tachykinin receptor 2 Homo sapiens 131-137 7651369-5 1995 [3H]Win 55212-2 specific binding to the CB2 receptor was moderately enhanced by divalent and monovalent cations but was only slightly inhibited by guanosine-5"-O-(3-thio)-triphosphate. Tritium 1-3 cannabinoid receptor 2 Homo sapiens 40-43 2172466-5 1990 Receptor interaction of the delta-opioid [3H](D-Pen2,D-Pen5)enkephalin was modulated by lipids to a greater extent than that of [3H]DAMGO: saturable binding was abolished by both oleic and elaidic acids. Tritium 42-45 presenilin enhancer, gamma-secretase subunit Homo sapiens 48-52 7651369-6 1995 Competition for [3H]Win 55212-2 specific binding to the CB2 receptor was stereoselective, with the following rank order of potency for the more active stereoisomers: HU-210 > (-)-CP-55940 approximately Win 55212-2 >> (-)delta 9-THC > anandamide. Tritium 17-19 cannabinoid receptor 2 Homo sapiens 56-59 2172466-5 1990 Receptor interaction of the delta-opioid [3H](D-Pen2,D-Pen5)enkephalin was modulated by lipids to a greater extent than that of [3H]DAMGO: saturable binding was abolished by both oleic and elaidic acids. Tritium 42-44 presenilin enhancer, gamma-secretase subunit Homo sapiens 48-52 8212085-3 1993 The L2 cell glucocorticoid receptor exhibited specific binding of [3H]TA which was saturable and appeared to be a single species of binding sites with an apparent KD = 4.9 +/- 3.7 nM and Bmax = 395.4 +/- 84.4 fmol/mg protein. Tritium 67-69 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 12-35 7629073-9 1995 When expressed in COS7 cells as a secreted protein A fusion protein, the catalytic domain of clone 16 displays alpha-1,2-mannosidase activity using [3H]mannose-labeled Man9GlcNAc as substrate, but the corresponding region of clone 4 is poorly secreted under identical conditions. Tritium 149-151 testis-specific serine kinase substrate Mus musculus 225-232 8325896-4 1993 Inhibition of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase, the rate-limiting enzyme in isoprenoid and sterol biosynthesis, by the cholesterol-lowering drug, lovastatin, blocks Fc epsilon RI-dependent [3H] serotonin ([3H]5HT) release from the mast cell line, RBL-2H3. Tritium 215-217 3-hydroxy-3-methylglutaryl-CoA reductase Rattus norvegicus 14-71 8325896-4 1993 Inhibition of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase, the rate-limiting enzyme in isoprenoid and sterol biosynthesis, by the cholesterol-lowering drug, lovastatin, blocks Fc epsilon RI-dependent [3H] serotonin ([3H]5HT) release from the mast cell line, RBL-2H3. Tritium 231-233 3-hydroxy-3-methylglutaryl-CoA reductase Rattus norvegicus 14-71 2293082-4 1990 Pre-treatment of pentobarbital prevented an increase in the [3H]PDBu binding activity in the stratum oriens of the CA1 subfield at 7 days after ischemia. Tritium 61-63 carbonic anhydrase 1 Homo sapiens 115-118 7619858-7 1995 However, when apo A-I or apo A-IV protein concentration was kept constant and supplemented with apo A-II, a lower [3H]FC efflux was found only at 1:3 M/M of apo A-I/A-II. Tritium 115-117 apolipoprotein A2 Homo sapiens 96-104 2146743-3 1990 The CD45 protein incorporated [3H]myristate, but little [3H]palmitate. Tritium 31-33 protein tyrosine phosphatase receptor type C Homo sapiens 4-8 7540662-0 1995 Phospholipase A2 down-regulates the affinity of [3H]AMPA binding to rat cortical membranes. Tritium 49-51 phospholipase A2 group IB Rattus norvegicus 0-16 7540662-1 1995 The effects of exogenous phospholipase A2 on the binding of alpha-[3H]amino-3-hydroxy-5-methylisoxazole-4-propionate ([3H]AMPA) to rat cortical membranes in the presence of the chaotrope potassium thiocyanate were assessed. Tritium 67-69 phospholipase A2 group IB Rattus norvegicus 25-41 8407286-5 1993 Moreover, the release of [3H]serotonin from IgE-sensitized mast cells showed a time-course more rapid than PAF production and occurred in cells sensitized with IgE at concentrations lower than those required for PAF formation. Tritium 26-28 PCNA clamp associated factor Rattus norvegicus 212-215 2213008-1 1990 Specific binding sites with pharmacological properties typical of serotonin 5-HT3 receptors were identified in membranes of the murine hybridoma cell line NG 108-15, using [3H]zacopride as a ligand. Tritium 173-175 hypothermia due to alcohol sensitivity 3 Mus musculus 78-81 7559085-3 1995 Incubation of NIM 1 cells with IL-1 alpha for 48 h increased the incorporation of 3H-thymidine (3H-TdR). Tritium 82-84 interleukin 1 alpha Homo sapiens 31-41 2213008-7 1990 Chromatographic analyses using Sephacryl S-400 and wheat germ agglutinin-agarose columns indicated marked enrichment (by 2.5- and 10-fold, respectively) in [3H]zacopride specific binding activity in the corresponding eluates compared with the starting soluble extract, a finding suggesting that both steps are of potential interest for the partial purification of solubilized 5-HT3 receptors. Tritium 157-159 hypothermia due to alcohol sensitivity 3 Mus musculus 378-381 2173748-1 1990 We investigated the mechanism and modulation of 3H-labeled leukotriene B4 (LTB4) binding to guinea pig lung membranes. Tritium 48-50 prostaglandin reductase 1 Cavia porcellus 75-79 2173748-2 1990 [3H] LTB4 bound specifically and rapidly (Kobs = 0.06 +/- 0.006 min-1) to high affinity (Kd = 0.63 +/- 0.06 nM) and saturable (Bmax = 224 +/- 16 fmol/mg) sites without cooperativity (nH = 1.05). Tritium 1-3 prostaglandin reductase 1 Cavia porcellus 5-9 2145079-2 1990 Depletion of CSF-1 from the culture medium of bone marrow cell-derived macrophages (BMM) resulted in a simultaneous decrease in the total (cell surface + intracellular) amount of TfR and complete cessation of proliferating activity [( 3H]thymidine incorporation). Tritium 235-237 colony stimulating factor 1 (macrophage) Mus musculus 13-18 8105069-9 1993 Furthermore, our autoradiographic data suggest that post-ischaemic alteration in [3H]cAMP binding between the hippocampal CA1 sector and striatum may be produced by different mechanisms. Tritium 82-84 carbonic anhydrase 1 Homo sapiens 122-125 7687062-6 1993 The actual binding site for SR-48,968 was localized to this region of the NK2 receptor by use of [3H]SR-48,968, which did not bind to the NK1 receptor but bound with similar high affinities to the wild-type NK2 receptor and to the chimeric NK1 receptor with the NK2 receptor segment incorporated around transmembrane segments VI and VII, Kd = 1.5 nM and 1.0 nM, respectively. Tritium 98-100 tachykinin receptor 2 Homo sapiens 74-86 8104321-1 1993 In the concentration range of 1-10 nM, neuromedin N produced a significant concentration-related increase in the Kd values of [3H]L-(-)-N-propylnorapomorphine binding sites in rat neostriatal membranes with a peak action at 10 nM (36% increase versus the control group mean value). Tritium 127-129 neurotensin Rattus norvegicus 39-51 7559085-6 1995 These stimulatory effects of IL-1 alpha on both 3H-TdR incorporation and 45Ca influx were similarly inhibited by nicardipine, an inhibitor of voltage-dependent Ca2+ channels, in a concentration-dependent manner (10-1000 nM). Tritium 48-50 interleukin 1 alpha Homo sapiens 29-39 8490029-4 1993 Incorporation of [3H]Ile into PTHRP in cell extracts increased over 20 min during pulse labeling and then remained constant throughout the incubation period up to 6 h. In contrast, the release of [3H]PTHRP into culture medium increased progressively over 6 h. Addition of cycloheximide or unlabeled Ile almost completely blocked incorporation of [3H]Ile into newly synthesised PTHRP. Tritium 18-20 parathyroid hormone-like hormone Rattus norvegicus 30-35 8490029-4 1993 Incorporation of [3H]Ile into PTHRP in cell extracts increased over 20 min during pulse labeling and then remained constant throughout the incubation period up to 6 h. In contrast, the release of [3H]PTHRP into culture medium increased progressively over 6 h. Addition of cycloheximide or unlabeled Ile almost completely blocked incorporation of [3H]Ile into newly synthesised PTHRP. Tritium 197-199 parathyroid hormone-like hormone Rattus norvegicus 200-205 2223825-5 1990 [3H]Mannose-labelled transferrin receptor was isolated from NS-1 cells by immunoprecipitation followed by electroelution from SDS polyacrylamide gels. Tritium 1-3 transferrin Mus musculus 21-32 2223825-11 1990 Reverse-phase HPLC analysis of tryptic glycopeptides of the isolated [3H]mannose-labelled transferrin receptor gave three 3H-labelled peaks, indicating that all three potential N-glycosylation sites on the receptor are utilised. Tritium 70-72 transferrin Mus musculus 90-101 7556407-8 1995 The peptide displaced [3H]substance P (IC50 = 3.3 +/- 1.8 x 10(-7) M) from guinea-pig bronchial tachykinin NK1 sites. Tritium 23-25 tachykinin 1 Mus musculus 26-37 2223825-11 1990 Reverse-phase HPLC analysis of tryptic glycopeptides of the isolated [3H]mannose-labelled transferrin receptor gave three 3H-labelled peaks, indicating that all three potential N-glycosylation sites on the receptor are utilised. Tritium 122-124 transferrin Mus musculus 90-101 2213830-4 1990 Whereas tryptamine derivatives generally display little selectivity for the various populations of 5-HT receptors, N1-n-propyl-5-methoxy-alpha-methyltryptamine (3h) binds with significant affinity (Ki = 12 nM) and selectivity at 5-HT2 receptors relative to 5-HT1A (Ki = 7100 nM), 5-HT1B (Ki = 5000 nM), 5-HT1C (Ki = 120 nM), and 5-HT1D (Ki greater than 10,000 nM) receptors. Tritium 161-163 5-hydroxytryptamine receptor 2C Homo sapiens 303-309 8490029-4 1993 Incorporation of [3H]Ile into PTHRP in cell extracts increased over 20 min during pulse labeling and then remained constant throughout the incubation period up to 6 h. In contrast, the release of [3H]PTHRP into culture medium increased progressively over 6 h. Addition of cycloheximide or unlabeled Ile almost completely blocked incorporation of [3H]Ile into newly synthesised PTHRP. Tritium 197-199 parathyroid hormone-like hormone Rattus norvegicus 200-205 8490029-8 1993 Pulse-chase studies demonstrated a rapid decrease of newly synthesized PTHRP forms within cells after 20 min; there was, however, a progressive increase in [3H]PTHRP in conditioned culture medium. Tritium 157-159 parathyroid hormone-like hormone Rattus norvegicus 160-165 8489251-8 1993 Preincubation of cells with 500 nM TPA overnight resulted in the inhibition of insulin- and TPA-stimulated 2-[3H]DOG uptake. Tritium 110-112 insulin Canis lupus familiaris 79-86 7611431-4 1995 Stimulation with either PDGF or IGF-1 induced substantial increases in DNA synthesis and cell number, as reflected by [3H]thymidine incorporation, flow cytometry, and methylene blue staining. Tritium 119-121 insulin like growth factor 1 Bos taurus 32-37 8489251-14 1993 Significant decreases in insulin- and TPA-stimulated 2-[3H]DOG uptake occurred, however, with glucose. Tritium 56-58 insulin Canis lupus familiaris 25-32 2151294-2 1990 Although mean activity of the specific cholinergic marker enzyme choline acetyltransferase (ChAT) was markedly reduced by about 60% in frontal cortex in the 4 patient groups and by 80% or greater in amygdala of the Alzheimer"s and Down"s syndrome patients, [3H]vesamicol binding density was, on average, either normal or only slightly reduced as compared with the controls. Tritium 258-260 choline O-acetyltransferase Homo sapiens 65-90 2151294-2 1990 Although mean activity of the specific cholinergic marker enzyme choline acetyltransferase (ChAT) was markedly reduced by about 60% in frontal cortex in the 4 patient groups and by 80% or greater in amygdala of the Alzheimer"s and Down"s syndrome patients, [3H]vesamicol binding density was, on average, either normal or only slightly reduced as compared with the controls. Tritium 258-260 choline O-acetyltransferase Homo sapiens 92-96 7494090-6 1995 Utilizing [3H]FPP and recombinant Ha-ras p21 as substrates in the reaction, the FTase followed Michaelis-Menten kinetics with Km values of 1.0 and 7.69 microM for respectively [3H]FPP and recombinant Ha-ras p21. Tritium 11-13 H3 histone pseudogene 16 Homo sapiens 41-44 2393852-3 1990 The results showed a good correlation between the binding of gastrin to CCKB receptor [Kd 1.125 +/- 0.3 (SD) nM] and its ability to either induce ornithine decarboxylase activity [50% effective concentration, 0.6 +/- 0.3 nM] and [3H]-thymidine incorporation [50% effective concentration, 2 +/- 0.4 nM]. Tritium 230-232 gastrin Rattus norvegicus 61-68 2393852-6 1990 Furthermore, the 50% inhibitory concentration of CR1409 and CR1505 to inhibit gastrin stimulated ornithine decarboxylase activity (an early event involved in cell proliferation) and [3H]thymidine incorporation were in agreement with their constants of inhibition (Ki) on gastrin binding. Tritium 183-185 gastrin Rattus norvegicus 78-85 8504465-8 1993 Reproducibility was assessed by adding tritium labeled BP-7,10/8,9-tetrol (1500 fmol) to a urine sample previously identified to contain the tetrol at levels below the limit of detection of the fluorescence assay; a recovery of > 30% of the added radioactivity was achieved (510 +/- 64 fmol, mean +/- SD, n = 3). Tritium 39-46 BP7 Homo sapiens 55-59 7683558-4 1993 On a concentration basis, cytoplasmic GR mRNA in the NIL was similar to that found in the AL, and GR binding using [3H]dexamethasone (DEX) as ligand demonstrated similar concentrations of specific [3H]DEX binding in acutely isolated AL and NIL tissues. Tritium 116-118 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 98-100 8340608-7 1993 Similar effects of TGF alpha and TGF beta 1 have been observed on the glycosaminoglycan-([35S]sulfate incorporation) and proteoglycan level ([3H]leucin incorporation into decorin and biglycan). Tritium 142-144 transforming growth factor alpha Rattus norvegicus 19-28 8455032-1 1993 An 80-kDa protein labeled with [3H]myristic acid in C6 glioma and N1E-115 neuroblastoma cells has been identified as the myristoylated alanine-rich C kinase substrate (MARCKS protein) on the basis of its calmodulin-binding, acidic nature, heat stability, and immunochemical properties. Tritium 32-34 myristoylated alanine rich protein kinase C substrate Mus musculus 121-166 8455032-1 1993 An 80-kDa protein labeled with [3H]myristic acid in C6 glioma and N1E-115 neuroblastoma cells has been identified as the myristoylated alanine-rich C kinase substrate (MARCKS protein) on the basis of its calmodulin-binding, acidic nature, heat stability, and immunochemical properties. Tritium 32-34 myristoylated alanine rich protein kinase C substrate Mus musculus 168-174 8455032-2 1993 When C6 cells preincubated with [3H]myristate were treated with 200 nM 4 beta-12-O-tetradecanoylphorbol 13-acetate (beta-TPA), labeled MARCKS was rapidly increased in the soluble digitonin fraction (maximal, fivefold at 10 min) with a concomitant decrease in the Triton X-100-soluble membrane fraction. Tritium 33-35 myristoylated alanine rich protein kinase C substrate Mus musculus 135-141 1698033-4 1990 ANF inhibited [3H]thymidine uptake in a dose-dependent manner in mesangial cells (half-maximal response 10(-11) M; 47% maximal inhibitory effect) but had no effect in 3T3 fibroblasts. Tritium 15-17 natriuretic peptide A Rattus norvegicus 0-3 2167699-4 1990 Both IGF-I and insulin increased DNA synthesis (measured as [3H]thymidine incorporation into DNA) and cell number in a concentration-dependent fashion, but IGF-I was at least 20 to 60 times more potent than insulin in its mitogenic effects. Tritium 61-63 insulin Canis lupus familiaris 15-22 2167683-1 1990 To examine the relationship between PKBS, a 17-kD protein covalently photolabeled by [3H]PK 14105, and its association with peripheral-type benzodiazepine binding sites, rat adrenal mitochondrial fractions were photolabeled with [3H]PK 14105, solubilized in digitonin, and subjected to anion-exchange chromatography over Q-Sepharose. Tritium 86-88 translocator protein Rattus norvegicus 36-40 2167683-3 1990 Specific binding sites for [3H]Ro5-4864 and [3H]PK 11195 were also assayed and found to cofractionate with each other and in a manner which coincided with the photolabeled PKBS profile. Tritium 28-30 translocator protein Rattus norvegicus 172-176 2167683-3 1990 Specific binding sites for [3H]Ro5-4864 and [3H]PK 11195 were also assayed and found to cofractionate with each other and in a manner which coincided with the photolabeled PKBS profile. Tritium 45-47 translocator protein Rattus norvegicus 172-176 8474432-1 1993 The specific binding characteristics of the novel cholecystokinin (CCK)B/gastrin receptor-selective peptoid antagonist radioligand [3H]PD 140376 were investigated using membrane homogenates prepared from guinea pig cerebral cerebral cortex and gastric fundic mucosa. Tritium 132-134 cholecystokinin Cavia porcellus 67-70 8474432-4 1993 Pharmacological characterization of the [3H]PD 140376-labeled binding sites with a series of agonist and antagonist ligands selective for each of the CCK receptor subtypes demonstrated, in both preparations, an affinity profile consistent with that of the CCKB/gastrin receptor. Tritium 41-43 cholecystokinin Cavia porcellus 150-153 2384761-6 1990 Competition binding experiments showed that peptide YY (PYY) was equal to NPY in its ability to displace [3H]proprionyl-NPY, whereas rat and human pancreatic polypeptide were without effect up to a concentration of 10(-6) M. This suggests that, whereas PYY and NPY may compete for the same receptor(s), the pancreatic polypeptides probably act on a separate population of receptors. Tritium 106-108 peptide YY Rattus norvegicus 44-54 7494090-6 1995 Utilizing [3H]FPP and recombinant Ha-ras p21 as substrates in the reaction, the FTase followed Michaelis-Menten kinetics with Km values of 1.0 and 7.69 microM for respectively [3H]FPP and recombinant Ha-ras p21. Tritium 11-13 H3 histone pseudogene 16 Homo sapiens 207-210 2384761-6 1990 Competition binding experiments showed that peptide YY (PYY) was equal to NPY in its ability to displace [3H]proprionyl-NPY, whereas rat and human pancreatic polypeptide were without effect up to a concentration of 10(-6) M. This suggests that, whereas PYY and NPY may compete for the same receptor(s), the pancreatic polypeptides probably act on a separate population of receptors. Tritium 106-108 peptide YY Rattus norvegicus 56-59 7682074-3 1993 Measurements made in intact L1210 cells showed that the rate of egress of [3H]MTX and [3H]MTX+G1 was different depending upon whether [3H]-MTX and its polyglutamates were accumulated intracellularly following biosynthesis during growth (3 hr) in the presence of [3H]MTX or when cells were pulse loaded (5 min) with [3H]MTX or [3H]-MTX+G1 just prior to measurement of egress. Tritium 75-77 metaxin 1 Mus musculus 78-81 2198284-7 1990 [Acetyl-3H]O-acetyl groups were found at both the 7- and the 9-positions, indicating that both 7-O-acetyl GD3 and 9-O-acetyl GD3 were synthesized by the action of O-acetyltransferase(s) on endogenous GD3. Tritium 8-10 CAS1 domain containing 1 Homo sapiens 163-182 7682074-3 1993 Measurements made in intact L1210 cells showed that the rate of egress of [3H]MTX and [3H]MTX+G1 was different depending upon whether [3H]-MTX and its polyglutamates were accumulated intracellularly following biosynthesis during growth (3 hr) in the presence of [3H]MTX or when cells were pulse loaded (5 min) with [3H]MTX or [3H]-MTX+G1 just prior to measurement of egress. Tritium 87-89 metaxin 1 Mus musculus 90-93 7494090-6 1995 Utilizing [3H]FPP and recombinant Ha-ras p21 as substrates in the reaction, the FTase followed Michaelis-Menten kinetics with Km values of 1.0 and 7.69 microM for respectively [3H]FPP and recombinant Ha-ras p21. Tritium 177-179 H3 histone pseudogene 16 Homo sapiens 41-44 7682074-3 1993 Measurements made in intact L1210 cells showed that the rate of egress of [3H]MTX and [3H]MTX+G1 was different depending upon whether [3H]-MTX and its polyglutamates were accumulated intracellularly following biosynthesis during growth (3 hr) in the presence of [3H]MTX or when cells were pulse loaded (5 min) with [3H]MTX or [3H]-MTX+G1 just prior to measurement of egress. Tritium 87-89 metaxin 1 Mus musculus 90-93 7494090-6 1995 Utilizing [3H]FPP and recombinant Ha-ras p21 as substrates in the reaction, the FTase followed Michaelis-Menten kinetics with Km values of 1.0 and 7.69 microM for respectively [3H]FPP and recombinant Ha-ras p21. Tritium 177-179 H3 histone pseudogene 16 Homo sapiens 207-210 7682074-3 1993 Measurements made in intact L1210 cells showed that the rate of egress of [3H]MTX and [3H]MTX+G1 was different depending upon whether [3H]-MTX and its polyglutamates were accumulated intracellularly following biosynthesis during growth (3 hr) in the presence of [3H]MTX or when cells were pulse loaded (5 min) with [3H]MTX or [3H]-MTX+G1 just prior to measurement of egress. Tritium 87-89 metaxin 1 Mus musculus 90-93 7682074-3 1993 Measurements made in intact L1210 cells showed that the rate of egress of [3H]MTX and [3H]MTX+G1 was different depending upon whether [3H]-MTX and its polyglutamates were accumulated intracellularly following biosynthesis during growth (3 hr) in the presence of [3H]MTX or when cells were pulse loaded (5 min) with [3H]MTX or [3H]-MTX+G1 just prior to measurement of egress. Tritium 87-89 metaxin 1 Mus musculus 90-93 7682074-3 1993 Measurements made in intact L1210 cells showed that the rate of egress of [3H]MTX and [3H]MTX+G1 was different depending upon whether [3H]-MTX and its polyglutamates were accumulated intracellularly following biosynthesis during growth (3 hr) in the presence of [3H]MTX or when cells were pulse loaded (5 min) with [3H]MTX or [3H]-MTX+G1 just prior to measurement of egress. Tritium 87-89 metaxin 1 Mus musculus 90-93 7682074-3 1993 Measurements made in intact L1210 cells showed that the rate of egress of [3H]MTX and [3H]MTX+G1 was different depending upon whether [3H]-MTX and its polyglutamates were accumulated intracellularly following biosynthesis during growth (3 hr) in the presence of [3H]MTX or when cells were pulse loaded (5 min) with [3H]MTX or [3H]-MTX+G1 just prior to measurement of egress. Tritium 87-89 metaxin 1 Mus musculus 90-93 7682074-3 1993 Measurements made in intact L1210 cells showed that the rate of egress of [3H]MTX and [3H]MTX+G1 was different depending upon whether [3H]-MTX and its polyglutamates were accumulated intracellularly following biosynthesis during growth (3 hr) in the presence of [3H]MTX or when cells were pulse loaded (5 min) with [3H]MTX or [3H]-MTX+G1 just prior to measurement of egress. Tritium 87-89 metaxin 1 Mus musculus 90-93 7682074-3 1993 Measurements made in intact L1210 cells showed that the rate of egress of [3H]MTX and [3H]MTX+G1 was different depending upon whether [3H]-MTX and its polyglutamates were accumulated intracellularly following biosynthesis during growth (3 hr) in the presence of [3H]MTX or when cells were pulse loaded (5 min) with [3H]MTX or [3H]-MTX+G1 just prior to measurement of egress. Tritium 87-89 metaxin 1 Mus musculus 90-93 7682074-3 1993 Measurements made in intact L1210 cells showed that the rate of egress of [3H]MTX and [3H]MTX+G1 was different depending upon whether [3H]-MTX and its polyglutamates were accumulated intracellularly following biosynthesis during growth (3 hr) in the presence of [3H]MTX or when cells were pulse loaded (5 min) with [3H]MTX or [3H]-MTX+G1 just prior to measurement of egress. Tritium 87-89 metaxin 1 Mus musculus 90-93 7682074-3 1993 Measurements made in intact L1210 cells showed that the rate of egress of [3H]MTX and [3H]MTX+G1 was different depending upon whether [3H]-MTX and its polyglutamates were accumulated intracellularly following biosynthesis during growth (3 hr) in the presence of [3H]MTX or when cells were pulse loaded (5 min) with [3H]MTX or [3H]-MTX+G1 just prior to measurement of egress. Tritium 87-89 metaxin 1 Mus musculus 90-93 7682074-3 1993 Measurements made in intact L1210 cells showed that the rate of egress of [3H]MTX and [3H]MTX+G1 was different depending upon whether [3H]-MTX and its polyglutamates were accumulated intracellularly following biosynthesis during growth (3 hr) in the presence of [3H]MTX or when cells were pulse loaded (5 min) with [3H]MTX or [3H]-MTX+G1 just prior to measurement of egress. Tritium 87-89 metaxin 1 Mus musculus 90-93 7682074-3 1993 Measurements made in intact L1210 cells showed that the rate of egress of [3H]MTX and [3H]MTX+G1 was different depending upon whether [3H]-MTX and its polyglutamates were accumulated intracellularly following biosynthesis during growth (3 hr) in the presence of [3H]MTX or when cells were pulse loaded (5 min) with [3H]MTX or [3H]-MTX+G1 just prior to measurement of egress. Tritium 87-89 metaxin 1 Mus musculus 90-93 2376583-2 1990 Partially purified preparations of the human progestin receptor and the human and rat glucocorticoid receptor proteins were covalently charged with the synthetic progestin, [3H]promegestone, by photoaffinity labeling. Tritium 174-176 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 86-109 2166901-4 1990 On the other hand, treatment with Boc-Cys(Npys) inhibited the effect of several GTP analogs (GTP gamma S, guanylyl-imidodiphosphate, guanylyl)-(beta, gamma-methylene)-diphosphate, and GTP) on [3H]8-OH-DPAT and [3H]clonidine binding. Tritium 193-195 phosphoenolpyruvate carboxykinase 1 Rattus norvegicus 93-187 2167733-4 1990 In superfused vas deferens preparations, NPY (0.3 microM) inhibited the stimulus-evoked overflow of both ATP and [3H]-noradrenaline ([3H]-NA) at 2 Hz, but only the stimulus-evoked release of [3H]-NA at 20 Hz. Tritium 114-116 pro-neuropeptide Y Cavia porcellus 41-44 2167733-4 1990 In superfused vas deferens preparations, NPY (0.3 microM) inhibited the stimulus-evoked overflow of both ATP and [3H]-noradrenaline ([3H]-NA) at 2 Hz, but only the stimulus-evoked release of [3H]-NA at 20 Hz. Tritium 134-136 pro-neuropeptide Y Cavia porcellus 41-44 7682074-3 1993 Measurements made in intact L1210 cells showed that the rate of egress of [3H]MTX and [3H]MTX+G1 was different depending upon whether [3H]-MTX and its polyglutamates were accumulated intracellularly following biosynthesis during growth (3 hr) in the presence of [3H]MTX or when cells were pulse loaded (5 min) with [3H]MTX or [3H]-MTX+G1 just prior to measurement of egress. Tritium 87-89 metaxin 1 Mus musculus 90-93 7682074-3 1993 Measurements made in intact L1210 cells showed that the rate of egress of [3H]MTX and [3H]MTX+G1 was different depending upon whether [3H]-MTX and its polyglutamates were accumulated intracellularly following biosynthesis during growth (3 hr) in the presence of [3H]MTX or when cells were pulse loaded (5 min) with [3H]MTX or [3H]-MTX+G1 just prior to measurement of egress. Tritium 87-89 metaxin 1 Mus musculus 90-93 2167733-4 1990 In superfused vas deferens preparations, NPY (0.3 microM) inhibited the stimulus-evoked overflow of both ATP and [3H]-noradrenaline ([3H]-NA) at 2 Hz, but only the stimulus-evoked release of [3H]-NA at 20 Hz. Tritium 134-136 pro-neuropeptide Y Cavia porcellus 41-44 7562331-5 1995 EGF and PDGF-BB stimulated DNA synthesis in a concentration-dependent manner, as measured by [3H] thymidine incorporation. Tritium 94-96 epidermal growth factor Homo sapiens 0-3 2207701-0 1990 Neonatal 5,7-DHT lesions upregulate [3H]mesulergine-labelled spinal 5-HT1C binding sites in the rat. Tritium 37-39 5-hydroxytryptamine receptor 2C Rattus norvegicus 68-74 7682074-3 1993 Measurements made in intact L1210 cells showed that the rate of egress of [3H]MTX and [3H]MTX+G1 was different depending upon whether [3H]-MTX and its polyglutamates were accumulated intracellularly following biosynthesis during growth (3 hr) in the presence of [3H]MTX or when cells were pulse loaded (5 min) with [3H]MTX or [3H]-MTX+G1 just prior to measurement of egress. Tritium 87-89 metaxin 1 Mus musculus 90-93 7682074-3 1993 Measurements made in intact L1210 cells showed that the rate of egress of [3H]MTX and [3H]MTX+G1 was different depending upon whether [3H]-MTX and its polyglutamates were accumulated intracellularly following biosynthesis during growth (3 hr) in the presence of [3H]MTX or when cells were pulse loaded (5 min) with [3H]MTX or [3H]-MTX+G1 just prior to measurement of egress. Tritium 87-89 metaxin 1 Mus musculus 90-93 7682074-4 1993 In the former case, [3H]MTX egressed with a T1/2 of 15 +/- 2 min, while [3H]MTX+G1 egressed with a T1/2 of 50 +/- 9 min. Tritium 21-23 metaxin 1 Mus musculus 24-27 7682074-4 1993 In the former case, [3H]MTX egressed with a T1/2 of 15 +/- 2 min, while [3H]MTX+G1 egressed with a T1/2 of 50 +/- 9 min. Tritium 73-75 metaxin 1 Mus musculus 76-79 7682074-5 1993 In pulse loaded cells, both [3H]MTX and [3H]MTX+G1 egressed with a T1/2 of 3.5 +/- 0.5 min. Tritium 29-31 metaxin 1 Mus musculus 32-35 7682074-5 1993 In pulse loaded cells, both [3H]MTX and [3H]MTX+G1 egressed with a T1/2 of 3.5 +/- 0.5 min. Tritium 41-43 metaxin 1 Mus musculus 44-47 7682074-6 1993 The same rapid egress of [3H]MTX and [3H]MTX+G1 seen following pulse loading was also documented in cells grown for 3 hr in the presence of nonradioactive MTX to normalize conditions with respect to the intracellular accumulation of [3H]MTX polyglutamates seen during exposure of cells to [3H]MTX during growth. Tritium 26-28 metaxin 1 Mus musculus 29-32 7680039-6 1993 Labeling of K8/18 immunoprecipitates, isolated from G2/M-arrested cells, with [3H]galactose followed by beta-elimination showed that K8/18 glycosylation consisted of single N-acetylglucosamine residues. Tritium 79-81 keratin 8 Homo sapiens 12-17 7680039-6 1993 Labeling of K8/18 immunoprecipitates, isolated from G2/M-arrested cells, with [3H]galactose followed by beta-elimination showed that K8/18 glycosylation consisted of single N-acetylglucosamine residues. Tritium 79-81 keratin 8 Homo sapiens 133-138 1696651-5 1990 Bolus doses of Ang II (10(-12)-10(-7) mol) increased the efflux of 3H from the lungs. Tritium 67-69 angiogenin Rattus norvegicus 15-18 2193614-2 1990 Although these sites have similar pharmacology to D1 dopamine receptors, the high-affinity component of 3H-SCH-23390 binding in the choroid plexus has been identified as the 5-HT1c subtype of serotonin receptor. Tritium 104-106 5-hydroxytryptamine receptor 2C Homo sapiens 174-180 2163016-9 1990 These results demonstrate that both alpha 1a- and alpha 1b-adrenergic receptor subtypes activate formation of [3H]InsP, although the molecular mechanisms by which these responses occur remain to be determined. Tritium 111-113 adrenoceptor alpha 1B Homo sapiens 36-78 2158429-10 1990 [3H]Thymidine incorporation in the presence of epidermal growth factor (50 ng/ml) was stimulated 66% by IGF-II (300 ng/ml) in control cells compared with 220% in cells from hepatectomized animals. Tritium 1-3 insulin-like growth factor 2 Rattus norvegicus 104-110 7596173-5 1995 A specificity (Sp) and a sensitivity (Se) score was calculated to evaluate the recognition by PCNA staining of the S-phase cells, as defined by the 3H-labeling. Tritium 148-150 proliferating cell nuclear antigen Homo sapiens 94-98 2157704-2 1990 The addition of PGE2 to cultures of BAC1.2F5 cells resulted in the inhibition of CSF-1-induced [3H]thymidine incorporation and cell proliferation. Tritium 96-98 colony stimulating factor 1 (macrophage) Mus musculus 81-86 8098849-6 1993 Endothelin-1, ET-2, and ET-3 stimulated [3H]thymidine incorporation in a dose-dependent manner, the maximum order of potency (100 nM) was again ET-1 approximately equal to ET-2 > ET-3. Tritium 41-43 endothelin 2 Homo sapiens 14-18 8098849-6 1993 Endothelin-1, ET-2, and ET-3 stimulated [3H]thymidine incorporation in a dose-dependent manner, the maximum order of potency (100 nM) was again ET-1 approximately equal to ET-2 > ET-3. Tritium 41-43 endothelin 3 Homo sapiens 24-28 8431769-6 1993 Only the latter phase of development of [3H]hemicholinium-3 binding corresponded to the ontogenetic changes in choline acetyltransferase activity; in the hippocampus, there were disparities even in young adulthood, where [3H]hemicholinium-3 binding showed a spike of activity centered around the 5th to 6th postnatal week, whereas choline acetyltransferase did not. Tritium 41-43 choline O-acetyltransferase Rattus norvegicus 111-136 2318984-7 1990 GRP (10 microM) significantly stimulated the release of the serous cell-specific product lactoferrin, and [3H]glucosamine-labeled glycoconjugates which are products of epithelial goblet cells and submucosal gland cells. Tritium 107-109 gastrin releasing peptide Homo sapiens 0-3 7596173-6 1995 The data show that in most instances the three antibodies recognized the 3H-labeled cells with high sensitivity, ie with few false negative, but with low specificity, ie with PCNA positivity extending to variable proportions of non-S-phase cells. Tritium 73-75 proliferating cell nuclear antigen Homo sapiens 175-179 7552267-2 1995 D4 receptor binding was defined as the difference between binding sites labeled by [3H]YM-09151-2 (D2 + D3 + D4 receptors) and those by [3H]raclopride, in the presence of 5"-guanylylimidodiphosphate (Gpp(NH)p) (D2 + D3 receptors). Tritium 84-86 dopamine receptor D4 Homo sapiens 0-11 1969500-2 1990 This 29-amino-acid peptide blocked [3H]thymidine uptake 30 to 50% by concanavalin A-stimulated CD4(+)--but not CD8(+)-enriched murine splenocytes. Tritium 36-38 CD4 antigen Mus musculus 95-98 8381602-2 1993 The in vitro binding of [3H]Sch 23390 to DA1 receptor sites on vascular smooth muscle cells was saturable. Tritium 25-27 immunoglobulin heavy diversity 4-11 (non-functional) Homo sapiens 41-44 8243108-4 1993 5-HT1C sites labelled with [3H]-mesulergine showed a distinct regional distribution: brainstem > diencephalon > cortex > hippocampus > cerebellum, constituting 65, 70, 31, 70, and 73% of total sites labelled by [3H]-mesulergine in the absence of 20 nM spiperone to block 5-HT2 sites, respectively. Tritium 28-30 5-hydroxytryptamine receptor 2C Rattus norvegicus 0-6 7552274-2 1995 In the dorsal hippocampus, [3H]YM-09151-2 bindings were high in the whole layers of CA1, the stratum pyramidale of CA4 and the stratum molecular of gyrus dentatus, moderate in the stratum oriens of CA3 and hilus of the gyrus dentatus, and low in remaining CA3 and the subiculum. Tritium 28-30 carbonic anhydrase 1 Rattus norvegicus 84-87 7786298-5 1995 A receptor binding study using radiolabelled [3H]WEB 2086 showed that Et-16-OCH3 exerts its actions through interaction with the PAF receptor. Tritium 46-48 PCNA clamp associated factor Homo sapiens 129-132 8486330-1 1993 We have studied the effects of neurotensin (NT) on the release of [3H]dopamine ([3H]DA) evoked by terminal depolarization with either K+, veratridine or 4-aminopyridine (4-AP). Tritium 67-69 neurotensin Rattus norvegicus 44-46 8486330-2 1993 NT (1-1000 nM) induced a net potentiation (up to 170%) of the K+ (25 mM)-evoked release of [3H]DA. Tritium 92-94 neurotensin Rattus norvegicus 0-2 7759504-6 1995 Similarly, when the GH-induced liberation of [3H]AA was blocked by the PLA2 inhibitor mepacrine or the Ca2+ channel blocker verapamil, GH-induced accumulation of P4502C12 mRNA was absent. Tritium 46-48 phospholipase A2 group IB Rattus norvegicus 71-75 8381251-4 1993 The rank order of specific nuclear uptake and binding of [3H]1,25-(OH)2D3 to the VDR was OMK > or = MLA144 >> B95-8. Tritium 58-60 vitamin D3 receptor Callithrix jacchus 81-84 7538124-5 1995 We identified the major glycosylation sites of K18 by comparing the tryptic 3H-glycopeptide pattern of the panel of mutant and wild type K18 expressed in the insect cells with the glycopeptides of K18 in human colonic cells. Tritium 76-78 keratin 18 Homo sapiens 47-50 7508036-2 1993 OLF was identified by its ability (a) to inhibit ouabain-sensitive 86Rb uptake into human erythrocytes, (b) to displace [3H]ouabain binding, and (c) to inhibit purified dog kidney Na-K-ATPase. Tritium 121-123 transmembrane O-mannosyltransferase targeting cadherins 1 Homo sapiens 0-3 7538756-5 1995 NG108-15 cells transfected to express either relatively high (clone beta N22) or low (clone beta N17) levels of beta 2-adrenoceptor both showed dose-dependent increases in specific [3H]forskolin binding in response to the beta-adrenoceptor agonist isoprenaline, and maximally effective concentrations of isoprenaline resulted in the generation of similar numbers of GSAC complexes in both clones. Tritium 182-184 adrenergic receptor, beta 2 Mus musculus 112-131 8235310-7 1993 CA 125 was demonstrated to incorporate [3H]-galactose but the level of radioactive incorporation was greatly reduced when WISH cells were incubated in the presence of phenyl N-acetyl-alpha-D-galactosaminide (an inhibitor of O-linked glycosylation) or monensin (an inhibitor of intracellular protein transport within the Golgi complex). Tritium 40-43 mucin 16, cell surface associated Homo sapiens 0-6 1360981-11 1992 Verapamil competitively inhibited P-gp-mediated accumulation of [3H]daunomycin but failed to alter the fluidity of CMV. Tritium 64-68 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 34-38 1335049-3 1992 LY223982 was found to be 189-fold more effective in displacing [3H]LTB4 than 35S-N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP) from their corresponding receptors on human neutrophils. Tritium 64-66 prostaglandin reductase 1 Cavia porcellus 67-71 1335049-4 1992 The concentration inhibiting 50% of response (IC50) for displacement of [3H]LTB4 (13.2 nM) was only 6.8-fold higher than the value for nonradioactive LTB4. Tritium 73-75 prostaglandin reductase 1 Cavia porcellus 76-80 7670227-2 1995 The two libraries were used in conjunction with an iterative selection process to identify individual peptides capable of inhibiting the binding of the mu-selective opioid peptide [3H]-[D-Ala2,MePhe4,Gly-ol5]enkephalin to rat brain homogenates. Tritium 181-183 proenkephalin Rattus norvegicus 208-218 1301395-0 1992 Biosynthetic labeling of beta-hexosaminidase B: inhibition of the cellular uptake of lysosomal secretions containing [3H]hexosaminidase B by insulin-like growth factor-II in rat C6 glial cells. Tritium 118-120 hexosaminidase subunit beta Rattus norvegicus 30-46 1301395-0 1992 Biosynthetic labeling of beta-hexosaminidase B: inhibition of the cellular uptake of lysosomal secretions containing [3H]hexosaminidase B by insulin-like growth factor-II in rat C6 glial cells. Tritium 118-120 hexosaminidase subunit beta Rattus norvegicus 121-137 1301395-0 1992 Biosynthetic labeling of beta-hexosaminidase B: inhibition of the cellular uptake of lysosomal secretions containing [3H]hexosaminidase B by insulin-like growth factor-II in rat C6 glial cells. Tritium 118-120 insulin-like growth factor 2 Rattus norvegicus 141-170 1301395-6 1992 When partially purified [3H]hexosaminidase B was incubated with rat C6 glial cells which express large numbers of IGF-II/mannose-6-phosphate receptors, the enzyme was taken up specifically via the IGF-II/mannose-6-phosphate receptor as evidenced by carbohydrate competition experiments. Tritium 25-27 hexosaminidase subunit beta Rattus norvegicus 28-44 1301395-6 1992 When partially purified [3H]hexosaminidase B was incubated with rat C6 glial cells which express large numbers of IGF-II/mannose-6-phosphate receptors, the enzyme was taken up specifically via the IGF-II/mannose-6-phosphate receptor as evidenced by carbohydrate competition experiments. Tritium 25-27 insulin-like growth factor 2 Rattus norvegicus 114-120 1301395-6 1992 When partially purified [3H]hexosaminidase B was incubated with rat C6 glial cells which express large numbers of IGF-II/mannose-6-phosphate receptors, the enzyme was taken up specifically via the IGF-II/mannose-6-phosphate receptor as evidenced by carbohydrate competition experiments. Tritium 25-27 insulin-like growth factor 2 Rattus norvegicus 197-203 7717977-4 1995 The affinities of 3H-labelled low-molecular-mass heparin and size-fractionated deca-, octa-, and hexa-saccharides for lipoprotein lipase immobilized on microtitre plates were determined from saturation curves. Tritium 18-20 lipoprotein lipase Homo sapiens 118-136 7664982-5 1995 Binding studies using the new labelled beta 3 adrenergic ligand [3H]SB 206606 showed a density of beta 3-AR in brown adipocyte plasma membranes comparable to that measured in vivo. Tritium 65-67 adrenoceptor beta 3 Homo sapiens 98-107 7702557-3 1995 Using this procedure, we have analysed the dose-dependent reduction of PP2A binding of [24-3H]OA by addition of OA, MCLR, calyculin-A and tautomycin. Tritium 91-93 protein phosphatase 2 phosphatase activator Homo sapiens 71-75 7702557-4 1995 The results indicate that the binding of the unlabelled inhibitors to the PP2A molecule causes a dramatic (10(6)-10(8)-fold) increase in the dissociation constant associated with the interaction of [24-3H]OA and PP2A. Tritium 202-204 protein phosphatase 2 phosphatase activator Homo sapiens 74-78 7702557-4 1995 The results indicate that the binding of the unlabelled inhibitors to the PP2A molecule causes a dramatic (10(6)-10(8)-fold) increase in the dissociation constant associated with the interaction of [24-3H]OA and PP2A. Tritium 202-204 protein phosphatase 2 phosphatase activator Homo sapiens 212-216 7900856-6 1995 MCP-1-induced inhibition of [3H]thymidine uptake was not affected in the presence of indomethacin (1 micrograms/ml) or NG-monomethyl-L-arginine (0.1 mM), and MCP-1 showed no effect on 6-ketoprostaglandin F1 alpha, guanosine 3",5"-cyclic monophosphate, and adenosine 3",5"-cyclic monophosphate syntheses in VSMC. Tritium 29-31 C-C motif chemokine ligand 2 Rattus norvegicus 0-5 7780191-0 1995 Tritium labelling of amino sugars at C-2 by alkaline epimerization in tritiated water. Tritium 0-7 complement C2 Homo sapiens 37-40 7780191-4 1995 The location of the incorporated tritium was established by treatment of the N-acetylhexosamines with borate under the conditions of the Morgan-Elson reaction, which converts the sugars to Kuhn"s chromogen I with concomitant loss of the C-2 hydrogen. Tritium 33-40 complement C2 Homo sapiens 237-240 7780191-5 1995 As expected, this treatment resulted in the formation of 3H2O, indicating that the tritium was located at C-2. Tritium 83-90 complement C2 Homo sapiens 106-109 7655707-3 1995 Their action on second messenger generation, and thus on cell proliferation, has been indirectly demonstrated by the decrease in [3H]-thymidine (TdR) uptake that follows co-stimulation of T-cells with mitogens and PTK inhibitors such as genistein (GEN). Tritium 130-132 protein tyrosine kinase 2 beta Homo sapiens 214-217 7857985-7 1995 Experiments with [3H]PAF indicate that human bone marrow cells actively metabolize this potent molecule by the deacetylation-transacylation pathway. Tritium 18-20 PCNA clamp associated factor Homo sapiens 21-24 7713168-2 1995 GR159897 inhibited binding of the NK2 receptor antagonist radioligand [3H]cyclohexylcarbonyl-Gly-Ala-(D)Trp-Phe-NMe2 ([3H]GR100679) to human ileum NK2 receptors transfected into Chinese hamster ovary cells (pKi 9.5) and to rat colon membranes (pKi 10.0). Tritium 71-73 NME/NM23 nucleoside diphosphate kinase 2 Homo sapiens 112-116 7713168-2 1995 GR159897 inhibited binding of the NK2 receptor antagonist radioligand [3H]cyclohexylcarbonyl-Gly-Ala-(D)Trp-Phe-NMe2 ([3H]GR100679) to human ileum NK2 receptors transfected into Chinese hamster ovary cells (pKi 9.5) and to rat colon membranes (pKi 10.0). Tritium 119-121 NME/NM23 nucleoside diphosphate kinase 2 Homo sapiens 112-116 7811467-8 1995 Relative to 1% fetal bovine serum (FBS), concentrations of IGF-1 between 3 and 30 ng/ml significantly increased the [3H]thymidine incorporation of fibroblasts derived from hyperoxic animals, whereas control cells were unresponsive to IGF-1 stimulation. Tritium 117-119 insulin-like growth factor 1 Rattus norvegicus 59-64 8619922-2 1995 Using the FDCP-Mix A4 multipotent stem cell line, MIP-1 alpha was shown to inhibit 1L-3 stimulated cell cycling (assessed using the [3H]-thymidine "suicide" assay). Tritium 133-135 chemokine (C-C motif) ligand 3 Mus musculus 50-61 8619922-3 1995 Furthermore, MIP-1 alpha can inhibit 1L-3-stimulated [3H]-thymidine incorporation in FDCP-Mix cells, with half maximal inhibition observed at 3 ng/ml MIP-1 alpha. Tritium 54-56 chemokine (C-C motif) ligand 3 Mus musculus 13-24 8619922-3 1995 Furthermore, MIP-1 alpha can inhibit 1L-3-stimulated [3H]-thymidine incorporation in FDCP-Mix cells, with half maximal inhibition observed at 3 ng/ml MIP-1 alpha. Tritium 54-56 chemokine (C-C motif) ligand 3 Mus musculus 150-161 8583251-4 1995 [3H] arachidonic acid was split, then recovered with cell nonesterified fatty acids and nonphosphorous glycerolipids after endocellular phospholipase A2 activity. Tritium 1-3 phospholipase A2 group IB Rattus norvegicus 136-152 7837796-6 1995 Cytochrome P450 2E1 mRNA was detected in liver tissues, using in situ hybridization with a 3H-labeled RNA probe coupled with quantitative image analysis. Tritium 91-93 cytochrome P450, family 2, subfamily e, polypeptide 1 Mus musculus 0-19 7899461-3 1995 EGF, IGF-I, and bFGF caused significant and dose-dependent increases in [3H]phosphatidic acid (PA) accumulation in the presence of propranolol, a phosphatidic acid phosphohydrolase inhibitor. Tritium 73-75 epidermal growth factor Rattus norvegicus 0-3 7899461-3 1995 EGF, IGF-I, and bFGF caused significant and dose-dependent increases in [3H]phosphatidic acid (PA) accumulation in the presence of propranolol, a phosphatidic acid phosphohydrolase inhibitor. Tritium 73-75 insulin-like growth factor 1 Rattus norvegicus 5-10 7479328-0 1995 Characterization and autoradiographic mapping of [3H]CP96,345, a nonpeptide selective NK1 receptor antagonist in guinea pig lung. Tritium 50-52 substance-P receptor Cavia porcellus 86-98 7479328-9 1995 [3H]CP96,345 may provide a useful tool to evaluate NK1 receptor expression in peripheral organs. Tritium 1-3 substance-P receptor Cavia porcellus 51-63 7778123-7 1995 MTx1 and MTx2 inhibited 3H-NMS binding to m1 and m3 receptors, with little effect on binding to m2 and m4 receptors. Tritium 24-26 metaxin 1 Homo sapiens 0-4 1491691-11 1992 TGF-alpha was found to stimulate [3H] thymidine incorporation into DNA and increase cellular proliferation of peritubular cells from each developmental stage, while having no effect on Sertoli cells. Tritium 34-36 transforming growth factor alpha Rattus norvegicus 0-9 1361417-7 1992 In cortical areas Par1/Par2, [3H]L-689,560 was also significantly reduced in deep layers but only in rats receiving 6 ng volkensin. Tritium 30-32 coagulation factor II (thrombin) receptor Rattus norvegicus 18-22 7819095-1 1994 Haemoglobin was observed to inhibit the GDP/GTP-exchange activity of ras protein (ras p21) by measurement of [3H]GDP-dissociation activity in time- and dose-dependent manners. Tritium 110-112 H3 histone pseudogene 16 Homo sapiens 86-89 1332490-11 1992 Because desensitized cells still responded to ATP with an increase of Cai, the desensitization was controlled at a step prior to the activation of phospholipase C. Desensitization occurred by a reduction of BK receptor number; a 10-min BK pretreatment reduced [3H]BK binding to receptors by 70% (from 14,600 receptors/cell, Km = 5 nM, to 5,300). Tritium 261-263 kininogen 1 Bos taurus 207-209 1330218-6 1992 Interestingly, [3H]IP3 and [3H]rolipram binding in CA1 showed a transient reduction, by 30% and 20% (both p < 0.01 vs. control), respectively, in the early reperfusion period. Tritium 16-18 carbonic anhydrase 1 Homo sapiens 51-54 7819095-5 1994 Methaemoglobin also inhibited both [3H]GDP-dissociation and [32P]GTP-hydrolysis activities of ras p21 in a very similar manner to that by haemoglobin. Tritium 36-38 H3 histone pseudogene 16 Homo sapiens 98-101 1330218-6 1992 Interestingly, [3H]IP3 and [3H]rolipram binding in CA1 showed a transient reduction, by 30% and 20% (both p < 0.01 vs. control), respectively, in the early reperfusion period. Tritium 28-30 carbonic anhydrase 1 Homo sapiens 51-54 7699198-5 1994 Transcription kinetics of the beta-actin and c-myc genes in serum-stimulated fibrosarcoma HT-1080 cells determined by [3H]uridine incorporation were comparable to that determined by the nuclear run-off method. Tritium 119-121 MYC proto-oncogene, bHLH transcription factor Homo sapiens 45-50 1489666-2 1992 In the presence of 0.5% ethanol, ET1 caused a time- and dose-dependent increase in the production of [3H]phosphatidylethanol ([3H]PEt). Tritium 102-104 endothelin-1 Oryctolagus cuniculus 33-36 7720146-1 1994 3H-thymidine incorporation (as a proliferation maker) confirms that the frozen bovine RPE cells release a resolvable material which can promote the proliferation of RPE cells, and the molecular weight of the material is more than 10,000 Daltons. Tritium 0-2 ribulose-phosphate 3-epimerase Bos taurus 86-89 1336746-6 1992 The 5-HT2/5-HT1c agonist DOI (3 x 10(-7) mol/l) decreased the evoked release of tritium, an effect which was prevented by ketanserin (10(-7) mol/l). Tritium 80-87 5-hydroxytryptamine receptor 2C Rattus norvegicus 10-16 7720146-1 1994 3H-thymidine incorporation (as a proliferation maker) confirms that the frozen bovine RPE cells release a resolvable material which can promote the proliferation of RPE cells, and the molecular weight of the material is more than 10,000 Daltons. Tritium 0-2 ribulose-phosphate 3-epimerase Bos taurus 165-168 7524656-2 1994 The stable analog [D-Trp6]GnRH (GnRHa) stimulated [3H]AA release from prelabeled cells after a lag of 1-2 min. Tritium 51-53 gonadotropin releasing hormone 1 Mus musculus 26-30 1331121-5 1992 The secondary elevation in DAG was coincident with a rise in 3H-phosphorylcholine (PC) and 3H-phosphorylethanolamine (PE) suggesting that PAF stimulates delayed phospholipase activities which hydrolyze alternate phospholipids besides the polyphosphoinositides. Tritium 61-63 PCNA clamp associated factor Rattus norvegicus 138-141 1331121-6 1992 This PAF-stimulated elevation in 3H-water soluble phosphorylbases was seen at 5 min but not at 15 sec suggesting that the initial rise in DAG as well as the initial elevation in [Ca2+]i are due primarily to PtdIns-4,5-P2 hydrolysis. Tritium 33-35 PCNA clamp associated factor Rattus norvegicus 5-8 7842510-6 1994 In addition, with age in both treatment groups, the molecular and granular layers of the cerebellum and the CA1 and dentate gyrus of the hippocampus displayed increasing [3H]CHA density. Tritium 171-173 carbonic anhydrase 1 Rattus norvegicus 108-111 1301386-4 1992 In both of these sub-populations Ang II treatment significantly increased the levels of 3H-labelled inositol phosphates as well as the total mass of inositol 1,4,5-triphosphate. Tritium 88-90 angiogenin Rattus norvegicus 33-36 1470308-5 1992 PYY, NPY and NPY13-36 each displaced 100% of 3H-NPY from rat brain membrane binding sites both in the absence and presence of 1 mM benextramine. Tritium 45-47 peptide YY Rattus norvegicus 0-3 7877752-3 1994 Fibronectin antibodies stain proliferative cells (simultaneously labelled by [3H]thymidin) of astrocytic type (double-immunostained by GFAP antibodies). Tritium 78-80 fibronectin 1 Rattus norvegicus 0-11 1470308-7 1992 Thus the selectivity of the benextramine-insensitive 3H-NPY binding site population--PYY > = NPY > NPY13-36 >> [Leu31,Pro34]NPY--is characteristic of a Y2-like NPY binding site population, while the benextramine-sensitive 3H-NPY binding sites appear to be a Y1-like binding site population. Tritium 53-55 peptide YY Rattus norvegicus 85-88 1470308-7 1992 Thus the selectivity of the benextramine-insensitive 3H-NPY binding site population--PYY > = NPY > NPY13-36 >> [Leu31,Pro34]NPY--is characteristic of a Y2-like NPY binding site population, while the benextramine-sensitive 3H-NPY binding sites appear to be a Y1-like binding site population. Tritium 234-236 peptide YY Rattus norvegicus 85-88 7929167-4 1994 However, unlike GLUT1, photoactivation of the [3H]forskolin-GalP complex fails to incorporate radioactivity covalently into the protein, in contrast to the effective incorporation of radioactivity from [3H]cytochalasin B into both proteins. Tritium 47-49 galanin like peptide Homo sapiens 60-64 1524445-2 1992 To determine if biotin bound to avidin is available to the chicken embryo, the fate of [3H]biotin complexed to avidin was monitored during embryonic development. Tritium 88-90 avidin Gallus gallus 111-117 7929167-7 1994 Equilibrium binding studies using membranes from an E. coli strain that overexpresses GalP reveal a single set of high affinity binding sites for [3H]forskolin with a Kd of 1.3-1.4 microM, probably forming a 1:1 complex, compared with a value of 7.5 microM for GLUT1. Tritium 147-149 galanin like peptide Homo sapiens 86-90 1331689-2 1992 The Scatchard analysis of the specific binding of [3H]neurotensin indicated a Kd value of 0.45 +/- 0.08 nM and a Bmax value of 3.27 +/- 0.29 pmol/mg of protein. Tritium 51-53 neurotensin Rattus norvegicus 54-65 1452414-12 1992 Down-modulation of CD3 expression on CD4+ and CD8+ cells was apparent as early as 1 h after treatment with less than 10% of CD4+ and CD8+ cells expressing CD3 by 12 h. Induction of IL-2R expression and IL-2-driven 3H-TdR incorporation was maximal at 12 h after anti-CD3. Tritium 214-216 CD4 antigen Mus musculus 37-40 1441230-9 1992 Finally, bTP-1 and bIFN-alpha I1 had bimodal effects on incorporation of [3H]TdR by IL-2-induced lymphocytes. Tritium 74-76 interleukin 2 Bos taurus 84-88 1331768-2 1992 PLMT activity was assayed by measuring the rate of incorporation of 3H-methyl groups from S-adenosyl-L-[methyl-3H] methionine into phospholipids. Tritium 68-70 phosphatidylethanolamine N-methyltransferase Rattus norvegicus 0-4 7834189-0 1994 Up-regulation of [3H]-des-Arg10-kallidin binding to the bradykinin B1 receptor by interleukin-1 beta in isolated smooth muscle cells: correlation with B1 agonist-induced PGI2 production. Tritium 18-20 B1 bradykinin receptor Oryctolagus cuniculus 56-78 1326626-3 1992 The EC50 value of endothelin-1, both for the induction of [3H]IP1 accumulation and for the positive inotropic effect was 6 x 10(-9) M. The endothelin-induced positive inotropic effect was linearly related to [3H]IP1 accumulation. Tritium 59-61 endothelin-1 Oryctolagus cuniculus 18-30 7926489-6 1994 However, IL-6 (10 ng/mL) plus a subthreshold concentration of human recombinant IL-1 beta significantly suppressed 3H release, and this was abolished by adding anti-IL-6 antibody or an IL-1 receptor antagonist. Tritium 115-117 interleukin 1 receptor antagonist Homo sapiens 185-209 1326626-3 1992 The EC50 value of endothelin-1, both for the induction of [3H]IP1 accumulation and for the positive inotropic effect was 6 x 10(-9) M. The endothelin-induced positive inotropic effect was linearly related to [3H]IP1 accumulation. Tritium 209-211 endothelin-1 Oryctolagus cuniculus 18-30 8077234-4 1994 TGF-alpha, AR, HB-EGF, and EGF were equipotent in stimulating [3H]thymidine incorporation by RIE-1 cells and bound the EGFr with equivalent affinity. Tritium 63-65 epidermal growth factor Homo sapiens 18-21 1497905-4 1992 Biochemical analysis using the mucin precursors [3H]glucosamine and [14C]serine indicated that CL2 cells can synthesize high-molecular-weight (M(r) greater than 200 kD) glycoprotein molecules that can be immunoprecipitated by this mucin-specific monoclonal antibody. Tritium 49-51 endogenous retrovirus group W member 5 Homo sapiens 95-98 1504008-3 1992 In the calf uterine cytosol, the progestins and the antiprogestins appeared to interact with the same PR as revealed by the displacement of [3H]R5020 by all of the above steroids. Tritium 141-143 progesterone receptor Bos taurus 102-104 7529387-4 1994 Binding of [3H]SP to mouse spinal cord membranes was inhibited by SP, CP-96,345, and to a lesser extent by SP(5-11), but not SP(1-7), consistent with these binding sites being NK-1 receptors. Tritium 12-14 tachykinin 1 Mus musculus 66-68 7529387-4 1994 Binding of [3H]SP to mouse spinal cord membranes was inhibited by SP, CP-96,345, and to a lesser extent by SP(5-11), but not SP(1-7), consistent with these binding sites being NK-1 receptors. Tritium 12-14 tachykinin 1 Mus musculus 176-180 7529387-6 1994 However, injection of 1 nmol of SP(1-7) decreased the Bmax of [3H]SP binding in the spinal cord at 6 h after its injection just as this dose of SP decreased the Bmax at 24 h. These data suggest that the N-terminus of SP is responsible for down-regulation of NK-1 binding. Tritium 63-65 tachykinin 1 Mus musculus 32-34 1327822-3 1992 Aglafoline also inhibited [3H]PAF (3.6 nM) binding to washed rabbit platelets with an IC50 value of 17.8 +/- 2.6 microM. Tritium 27-29 PCNA clamp associated factor Rattus norvegicus 30-33 7529387-6 1994 However, injection of 1 nmol of SP(1-7) decreased the Bmax of [3H]SP binding in the spinal cord at 6 h after its injection just as this dose of SP decreased the Bmax at 24 h. These data suggest that the N-terminus of SP is responsible for down-regulation of NK-1 binding. Tritium 63-65 tachykinin 1 Mus musculus 66-68 7529387-6 1994 However, injection of 1 nmol of SP(1-7) decreased the Bmax of [3H]SP binding in the spinal cord at 6 h after its injection just as this dose of SP decreased the Bmax at 24 h. These data suggest that the N-terminus of SP is responsible for down-regulation of NK-1 binding. Tritium 63-65 tachykinin 1 Mus musculus 66-68 7529387-6 1994 However, injection of 1 nmol of SP(1-7) decreased the Bmax of [3H]SP binding in the spinal cord at 6 h after its injection just as this dose of SP decreased the Bmax at 24 h. These data suggest that the N-terminus of SP is responsible for down-regulation of NK-1 binding. Tritium 63-65 tachykinin 1 Mus musculus 66-68 1319829-7 1992 In primary tumor cell cultures, GRP increased the incorporation of [3H] thymidine in DNA in a dose- and time-dependent manner. Tritium 68-70 gastrin releasing peptide Rattus norvegicus 32-35 7528680-7 1994 The channel-site ligand [3H]MK 801 ((+)-5-methyl-10,11-dihydro-5H- dibenzo[a,d]cyclo-hepten-5,10-imine hydrogen maleate) showed the affinity ranking NR1-NR2A = NR1-NR2B >> NR1 > NR1-NR2C = NR1-NR2D. Tritium 25-27 glutamate ionotropic receptor NMDA type subunit 2D Homo sapiens 202-206 1612026-7 1992 The protein kinase-C (PKC) activator phorbol 12-myristate 13-acetate increased [3H]thymidine incorporation, and TGF beta inhibited this action of phorbol 12-myristate 13-acetate. Tritium 80-82 protein kinase C, gamma Rattus norvegicus 4-20 1612026-7 1992 The protein kinase-C (PKC) activator phorbol 12-myristate 13-acetate increased [3H]thymidine incorporation, and TGF beta inhibited this action of phorbol 12-myristate 13-acetate. Tritium 80-82 protein kinase C, gamma Rattus norvegicus 22-25 8078497-10 1994 The affinity of [3H]SB 206606 is 76 times higher for the beta 3-adrenergic receptor than for the beta 1/beta 2-adrenergic receptors, thus allowing, under controlled conditions, measurement of interactions only with the beta 3-adrenergic receptor in complex membrane systems. Tritium 17-19 beta-2 adrenergic receptor Cricetulus griseus 97-131 8078499-3 1994 [3H]Bryostatin 1 showed lower affinity for PKC-beta 1 and -gamma, compared with PKC-alpha, -delta, -epsilon, and -eta. Tritium 1-3 protein kinase C gamma Homo sapiens 43-64 8058062-11 1994 When the AHD-M1 and AHD-2 cDNAs were inserted into the expression vector pSG5 and transfected into cultured COS cells, 3-5-fold and 100-fold increases, respectively, in the conversion of [3H]retinaldehyde to [3H]retinoic acid could be detected by high performance liquid chromatographic assay. Tritium 188-190 aldehyde dehydrogenase 2, mitochondrial Mus musculus 9-15 1320691-2 1992 Disulfiram displaced specific binding of [3H]PK 11195 from PBR in the cerebral cortex with an IC50 value of 5 x 10(-7) M. The binding of [3H]PK 11195 and [3H]Ro 5-4864 to PBR in the kidney was displaced by disulfiram with IC50 values of 7 x 10(-7) and 2 x 10(-7) M, respectively. Tritium 42-44 translocator protein Rattus norvegicus 59-62 1320692-1 1992 In the present studies, the pharmacology of the leukotriene B4 (LTB4) receptor on intact human polymorphonuclear neutrophils (PMN) was characterized using radioligand binding techniques with [3H]LTB4 and a novel LTB4 receptor antagonist radioligand [3H]CGS 23131 (LY223982). Tritium 192-194 leukotriene B4 receptor Homo sapiens 48-78 8058062-11 1994 When the AHD-M1 and AHD-2 cDNAs were inserted into the expression vector pSG5 and transfected into cultured COS cells, 3-5-fold and 100-fold increases, respectively, in the conversion of [3H]retinaldehyde to [3H]retinoic acid could be detected by high performance liquid chromatographic assay. Tritium 209-211 aldehyde dehydrogenase 2, mitochondrial Mus musculus 9-15 1320692-1 1992 In the present studies, the pharmacology of the leukotriene B4 (LTB4) receptor on intact human polymorphonuclear neutrophils (PMN) was characterized using radioligand binding techniques with [3H]LTB4 and a novel LTB4 receptor antagonist radioligand [3H]CGS 23131 (LY223982). Tritium 250-252 leukotriene B4 receptor Homo sapiens 48-78 9968128-0 1992 Spin-triplet strength in the 3H(p Tritium 29-31 spindlin 1 Homo sapiens 0-4 1613751-5 1992 The C1-8 alkoxy-TZTP 5a-l analogues all displaced [3H]-Oxo-M and [3H]-Pz with low nanomolar affinity. Tritium 51-53 complement C2 Cavia porcellus 4-8 1613751-5 1992 The C1-8 alkoxy-TZTP 5a-l analogues all displaced [3H]-Oxo-M and [3H]-Pz with low nanomolar affinity. Tritium 51-54 complement C2 Cavia porcellus 4-8 7953635-4 1994 The density of [3H]CGS 21680 binding was greatest in the stratum radiatum of the CA1 area, followed by the stratum oriens of the cornu Ammonis, stratum radiatum of the CA3 are and supra-granular layer of the dentate gyrus. Tritium 16-18 carbonic anhydrase 1 Rattus norvegicus 81-84 1597146-5 1992 A role for diacylglycerol-PKC signalling in insulin-stimulated glucose transport was suggested by studies of [3H]2-deoxyglucose ([3H]2-DOG) uptake in the rat soleus in vitro in that 1) PKC translocation and 2-DOG uptake were correlated; and 2) stimulatory effects of insulin and phorbol esters on 2-DOG uptake were apparently nonadditive. Tritium 110-112 protein kinase C, beta Rattus norvegicus 26-29 1597146-5 1992 A role for diacylglycerol-PKC signalling in insulin-stimulated glucose transport was suggested by studies of [3H]2-deoxyglucose ([3H]2-DOG) uptake in the rat soleus in vitro in that 1) PKC translocation and 2-DOG uptake were correlated; and 2) stimulatory effects of insulin and phorbol esters on 2-DOG uptake were apparently nonadditive. Tritium 110-112 insulin Canis lupus familiaris 44-51 8206972-6 1994 Heparin, which displaces LPL from binding sites on cell surface proteoglycans, increased [3H]retinoid uptake by an additional 2-fold. Tritium 90-92 lipoprotein lipase Oryctolagus cuniculus 25-28 1597146-5 1992 A role for diacylglycerol-PKC signalling in insulin-stimulated glucose transport was suggested by studies of [3H]2-deoxyglucose ([3H]2-DOG) uptake in the rat soleus in vitro in that 1) PKC translocation and 2-DOG uptake were correlated; and 2) stimulatory effects of insulin and phorbol esters on 2-DOG uptake were apparently nonadditive. Tritium 130-132 protein kinase C, beta Rattus norvegicus 26-29 8003510-8 1994 Insulin (1 microM) and FCS (9%) stimulate [3H]thymidine incorporation in parental CHO cells to a similar high level whereas in insulin receptor overexpressing CHO cells the maximum of insulin-stimulated [3H]thymidine incorporation is only 55% of the level reached by 9% FCS. Tritium 204-206 insulin receptor Cricetulus griseus 127-143 1597146-5 1992 A role for diacylglycerol-PKC signalling in insulin-stimulated glucose transport was suggested by studies of [3H]2-deoxyglucose ([3H]2-DOG) uptake in the rat soleus in vitro in that 1) PKC translocation and 2-DOG uptake were correlated; and 2) stimulatory effects of insulin and phorbol esters on 2-DOG uptake were apparently nonadditive. Tritium 130-132 insulin Canis lupus familiaris 44-51 8187073-4 1994 injection of [3H]Trp-P-1 (100 micrograms/kg) in NMRI mice treated with the cytochrome P4501A-inducing agent beta-naphthoflavone (BNF). Tritium 14-16 natriuretic peptide type B Mus musculus 108-133 1534843-7 1992 In cortical areas, Par1/Par2 [3H]8-OH-DPAT binding was significantly reduced (2 and 6 ng animals) in both superficial and deeper cortical layers, but quantitative histological analysis has not been performed. Tritium 30-32 coagulation factor II (thrombin) receptor Rattus norvegicus 19-23 8187073-7 1994 At incubation of tissues with [3H]Trp-P-1 (0.75 microM) there was also a selective binding of radioactive substance in endothelial cells of the lung and liver and in the vena cava from BNF-treated mice but not from vehicle-treated control mice. Tritium 31-33 natriuretic peptide type B Mus musculus 185-188 8187073-8 1994 Ellipticine but not alpha-naphthoflavone inhibited the endothelial binding in BNF-treated mice exposed to [3H]Trp-P-1 in vivo or in vitro. Tritium 107-109 natriuretic peptide type B Mus musculus 78-81 1641195-2 1992 The binding of 3H-5-HT was limited to 5-HT1D sites (5-CT-sensitive) by co-incubating pindolol and mesulergine with [3H]5-HT, and by using 10 microM 5-HT to determine non-specific binding. Tritium 15-17 5-hydroxytryptamine receptor 1D Homo sapiens 38-44 1641195-2 1992 The binding of 3H-5-HT was limited to 5-HT1D sites (5-CT-sensitive) by co-incubating pindolol and mesulergine with [3H]5-HT, and by using 10 microM 5-HT to determine non-specific binding. Tritium 116-118 5-hydroxytryptamine receptor 1D Homo sapiens 38-44 8187073-10 1994 Our data suggest an in situ metabolism of [3H]Trp-P-1 to a reactive species, catalyzed by an BNF-inducible P450 form, possibly 1A1, in endothelial cells. Tritium 43-45 natriuretic peptide type B Mus musculus 93-96 7523204-6 1994 Conditioned media (CM) from MD-IGF-I cells induced with DEX stimulated a 2.8-fold increase in [3H]thymidine incorporation into DNA of parental MAC-T cells, compared with uninduced cells. Tritium 95-97 insulin like growth factor 1 Bos taurus 31-36 1617007-8 1992 Insulin and TGF-alpha alone and in combination increased the number of cells labeled with [3H]thymidine, as assessed by autoradiography. Tritium 91-93 transforming growth factor alpha Rattus norvegicus 12-21 8047333-2 1994 The addition of EGF (10 ng/ml) to fresh DMEM: F12 medium with cultured basal keratinocytes stimulated [3H]thymidine incorporation into cells. Tritium 103-105 epidermal growth factor Homo sapiens 16-19 1643254-10 1992 Total DNA binding of [3H]5-MeC as well as formation of deoxyguanosine adducts exceeded that of [3H]5,6-diMeC by 3-4-fold. Tritium 22-24 chemokine (C-C motif) ligand 28 Mus musculus 27-30 8163469-4 1994 Both cell types rapidly increased [3H]inositol phosphates and [Ca2+]i in response to 1 microM NMB, whereas preincubation with 3 nM NMB for 3 h markedly attenuated the ability of 1 microM NMB, but not 1 microM endothelin-1, to alter either cell type"s biological activity. Tritium 35-37 neuromedin B Rattus norvegicus 94-97 1539670-2 1992 TGF-beta 1 inhibited [3H]thymidine incorporation stimulated by either the CCK analogue caerulein, epidermal growth factor, or insulin. Tritium 22-24 cholecystokinin Mus musculus 74-77 1324692-6 1992 The order of potency for inhibition of [3H]thymidine uptake by WEHI-3B and HL-60 cells was (R)-ET-16-OCH3-GPC = (S)-ET-16-OCH3-GPC greater than (S)-PAF greater than (R)-PAF; the EC50 values were 2, 2, 15, and greater than 40 microM, respectively. Tritium 40-42 glycophorin C (Gerbich blood group) Homo sapiens 106-109 8163469-4 1994 Both cell types rapidly increased [3H]inositol phosphates and [Ca2+]i in response to 1 microM NMB, whereas preincubation with 3 nM NMB for 3 h markedly attenuated the ability of 1 microM NMB, but not 1 microM endothelin-1, to alter either cell type"s biological activity. Tritium 35-37 neuromedin B Rattus norvegicus 131-134 1324692-6 1992 The order of potency for inhibition of [3H]thymidine uptake by WEHI-3B and HL-60 cells was (R)-ET-16-OCH3-GPC = (S)-ET-16-OCH3-GPC greater than (S)-PAF greater than (R)-PAF; the EC50 values were 2, 2, 15, and greater than 40 microM, respectively. Tritium 40-42 glycophorin C (Gerbich blood group) Homo sapiens 127-130 8163469-4 1994 Both cell types rapidly increased [3H]inositol phosphates and [Ca2+]i in response to 1 microM NMB, whereas preincubation with 3 nM NMB for 3 h markedly attenuated the ability of 1 microM NMB, but not 1 microM endothelin-1, to alter either cell type"s biological activity. Tritium 35-37 neuromedin B Rattus norvegicus 131-134 1310474-7 1992 Five days following fimbria-fornix lesion there was also a decrease (13-15%, P less than 0.05) in [3H]KA binding in CA3 strata radiatum and pyramidale. Tritium 99-101 carbonic anhydrase 3 Rattus norvegicus 116-119 1418862-6 1992 There was a high correlation between glial cell count and 3H-L-deprenyl binding with a relation indicating enhanced MAO-B protein in glial cells within areas of neurodegeneration. Tritium 58-60 monoamine oxidase B Homo sapiens 116-121 8150086-2 1994 We here show that both nicotine and cytisine stimulate [3H]serotonin release in a dose-dependent manner; this effect is antagonized by alpha-bungarotoxin (alpha Bgtx) and alpha-conotoxin MI (alpha Ctx). Tritium 56-58 cytochrome P450 family 27 subfamily A member 1 Homo sapiens 197-200 1560592-6 1992 PYY inhibited the [3H] ACh release from cholinergic nerve endings of gastric body evoked by electrical transmural stimulation. Tritium 19-21 peptide YY Rattus norvegicus 0-3 1720092-8 1991 coli or hIGFBP-3CHO potentiated subsequent IGF-I-stimulated [3H]AIB uptake. Tritium 61-63 IGFI Bos taurus 43-48 1720092-11 1991 After a 72-h preincubation, IGF-I cell binding remained increased 2-fold with commensurate enhancement of IGF-I-stimulated [3H]AIB uptake. Tritium 124-126 IGFI Bos taurus 28-33 1720092-11 1991 After a 72-h preincubation, IGF-I cell binding remained increased 2-fold with commensurate enhancement of IGF-I-stimulated [3H]AIB uptake. Tritium 124-126 IGFI Bos taurus 106-111 7514436-9 1994 IL-3 had antiproliferative activity against FSCCL as manifested by a decrease in [3H]thymidine incorporation and a decrease in the total number of cells after 72 hours of culture. Tritium 82-84 interleukin 3 Homo sapiens 0-4 1836757-5 1991 Transient expression of this clone demonstrated high-affinity binding of [3H]5-HT with a pharmacological profile corresponding to that of the 5-HT1B subtype: 5-CT, 5-HT greater than propranolol greater than methysergide greater than rauwolscine greater than 8-OH-DPAT. Tritium 74-76 5-hydroxytryptamine receptor 1B Rattus norvegicus 142-148 8206511-4 1994 Anti-mu-stimulated B cells responded to IL-2 by incorporation of [3H]thymidine and high rate immunoglobulin (Ig) secretion. Tritium 66-68 interleukin 2 Mus musculus 40-44 1792939-3 1991 In both actively growing and growth-arrested rat osteoblasts, LIF stimulated [3H]thymidine incorporation in a dose-dependent manner. Tritium 78-80 LIF, interleukin 6 family cytokine Rattus norvegicus 62-65 1792939-5 1991 LIF-treated cells showed reduced [3H]thymidine incorporation in comparison with control, as they approached confluence, possibly because of the increased cell density in the LIF-treated cultures. Tritium 34-36 LIF, interleukin 6 family cytokine Rattus norvegicus 0-3 1792939-6 1991 In UMR-106 cells, treatment with LIF inhibited [3H]thymidine incorporation in both actively growing and growth-arrested cultures. Tritium 48-50 LIF, interleukin 6 family cytokine Rattus norvegicus 33-36 8110183-1 1994 High-density lipoprotein (HDL)-[3H]triolein (i.e. [3H]triolein incorporated into reconstituted HDL) was taken up by cultured fibroblasts through an apparently saturable process, competitively inhibited by non-labelled HDL and independent of the LDL receptor. Tritium 32-34 low density lipoprotein receptor Homo sapiens 245-257 1777814-3 1991 In the present study, we show that unilateral lesion of the PBS increases the binding of the sulphated octapeptide of [3H]cholecystokinin ([3H]CCK) within the ipsilateral VMN of the hypothalamus, but not within other brain nuclei that contain receptors for CCK. Tritium 119-121 cholecystokinin Rattus norvegicus 143-146 1777814-3 1991 In the present study, we show that unilateral lesion of the PBS increases the binding of the sulphated octapeptide of [3H]cholecystokinin ([3H]CCK) within the ipsilateral VMN of the hypothalamus, but not within other brain nuclei that contain receptors for CCK. Tritium 119-121 cholecystokinin Rattus norvegicus 257-260 8206647-3 1994 Epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) added singly caused significant increases relative to control in both the uptake of [3H]thymidine into the cellular DNA of subconfluent monolayers and of [3H]proline into collagenase-sensitive protein. Tritium 157-159 myotrophin Rattus norvegicus 10-23 1777814-3 1991 In the present study, we show that unilateral lesion of the PBS increases the binding of the sulphated octapeptide of [3H]cholecystokinin ([3H]CCK) within the ipsilateral VMN of the hypothalamus, but not within other brain nuclei that contain receptors for CCK. Tritium 140-142 cholecystokinin Rattus norvegicus 143-146 1777814-3 1991 In the present study, we show that unilateral lesion of the PBS increases the binding of the sulphated octapeptide of [3H]cholecystokinin ([3H]CCK) within the ipsilateral VMN of the hypothalamus, but not within other brain nuclei that contain receptors for CCK. Tritium 140-142 cholecystokinin Rattus norvegicus 257-260 8309245-5 1994 Fourteen of the 18 cases with concomitant rises of 3H-TdR and 3H-araC incorporation into the DNA after GM-CSF had elevated DNA polymerase alpha activity (16-531%, median 72%) and in ten cases overall DNA polymerase activity was enhanced (10-70%, median 22.5%). Tritium 62-64 colony stimulating factor 2 Homo sapiens 103-109 8085173-4 1994 (3) Taking 3H-TdR incorporation as an index, it was observed that acetaminophen could reduce the DNA synthesis of hepatocytes, and pretreatment with EGF could reverse this effect, but, stimulation of DNA synthesis by EGF was not correlated with its hepatoprotection. Tritium 11-13 epidermal growth factor Mus musculus 149-152 1722410-1 1991 The possibility of increasing the activity of etoposide (VP-16) by combining this anti-cancer agent with indomethacin (Indo) was investigated by treating murine and human cultured tumor cells with a combination of Indo and VP-16 and quantitating VP-16 cytotoxicity by the [3H]thymidine incorporation assay. Tritium 273-275 host cell factor C1 Homo sapiens 57-62 1722410-10 1991 In other studies, Indo was found to significantly increase the steady state accumulation of [3H]-VP-16 in all five cell lines studied. Tritium 93-95 host cell factor C1 Homo sapiens 97-102 8050398-1 1994 Results are presented for proteins with known three-dimensional structure (lysozyme, myoglobin, ribonuclease), which show that the probability of label incorporation upon bombardment by "hot" tritium atoms may be quantitatively linked with the surface area of the protein accessible to water molecules. Tritium 192-199 myoglobin Homo sapiens 85-94 1913676-13 1991 In mice, after a single bolus injection of 10 mg/kg of ICI D1694, TS was inhibited greater than 80% for 24 h in ascitic L1210:NCI cells (as measured by the rate of 3H release from [5-3H]deoxyuridine). Tritium 164-166 thymidylate synthase Mus musculus 66-68 8026706-3 1994 Ginsenoside Rb1 Rb2, Rc, Re, Rf and Rg1 inhibited the specific [3H]muscimol binding to the high-affinity site. Tritium 64-66 protein phosphatase 1 regulatory subunit 3A Homo sapiens 36-39 1918089-2 1991 Agents known to reverse this pleiotropic drug resistance (chemosensitizers) have been shown to interact with P-gp; and as such, the inhibition of photoaffinity labeling by P-gp probes (such as [3H]azidopine) has been proposed as a basis for mass screening of chemosensitizers. Tritium 194-196 phosphoglycolate phosphatase Mus musculus 172-176 7510997-4 1994 Immunopurification from cytosol of [3H]steroid-labeled tungstate-stabilized PR with anti-PR immunoadsorbent yielded "9S"-PR species in which hsp90, hsp70 and p59/HBI were present. Tritium 36-38 heat shock protein 90 alpha family class A member 1 Homo sapiens 141-146 8295490-9 1994 The recovery rate of PAF throughout this procedure was constant and satisfactory (37.4 +/- 9.7%), which was confirmed using [3H]-PAF. Tritium 125-127 PCNA clamp associated factor Homo sapiens 21-24 1686763-5 1991 Since quinpirole has a reported Ki value of 5.1 nM for dopamine D3 receptors, similar to the quinpirole Ki value of 4.8 nM for the high-affinity state of the dopamine D2 receptor, it appears that [3H]quinpirole with its Kd of 3.9-6.8 nM could label both these two dopamine receptors. Tritium 197-200 dopamine receptor D2 Canis lupus familiaris 158-178 1840236-3 1991 A regional assignment of this locus to 4p14 has been made by in situ hybridization to metaphase chromosomes using both tritium and fluorescently labelled probes. Tritium 119-126 ribonuclease P/MRP subunit p14 Homo sapiens 40-43 1666367-2 1991 Insulin secretion and 3H efflux from myo-[2-3H]inositol-prelabelled islets (reflecting phosphoinositide hydrolysis) stimulated by CCK-8 (100 nM) were both inhibited by L-364,718, partially at 1 nM and totally at 10 nM. Tritium 22-24 cholecystokinin Rattus norvegicus 130-133 1894612-1 1991 The sterol binding specificity of rat recombinant liver fatty acid binding protein (L-FABP) and intestinal fatty acid binding protein (I-FABP) was characterized with [3H]cholesterol and a fluorescent sterol analog dehydroergosterol. Tritium 167-169 fatty acid binding protein 2 Rattus norvegicus 96-133 1894612-1 1991 The sterol binding specificity of rat recombinant liver fatty acid binding protein (L-FABP) and intestinal fatty acid binding protein (I-FABP) was characterized with [3H]cholesterol and a fluorescent sterol analog dehydroergosterol. Tritium 167-169 fatty acid binding protein 2 Rattus norvegicus 135-141 8295490-9 1994 The recovery rate of PAF throughout this procedure was constant and satisfactory (37.4 +/- 9.7%), which was confirmed using [3H]-PAF. Tritium 125-127 PCNA clamp associated factor Homo sapiens 129-132 8186462-5 1994 When NIH 3T3 cells prelabeled with [3H]inositol were synchronized by serum deprivation followed by stimulation with platelet-derived growth factor (PDGF), the amounts of labeled InsP5 and InsP6 began to increase only after 12 h of stimulation, when cells entered the S-phase as indicated by increased [3H]thymidine incorporation. Tritium 36-38 myotrophin Rattus norvegicus 133-146 1898326-2 1991 Yields of 14CO2 and 14C-labelled lactate, and amounts of 14C from [1-14C]mannose incorporated into glucose and of 3H bound to C-2 of glucose, were measured. Tritium 114-116 complement component 2 (within H-2S) Mus musculus 126-129 8008632-5 1994 Corticotropin-releasing factor had no effect on cytosolic calcium but stimulated [3H]arachidonic acid release from NCI-H1299 cells with an ED50 of 30 nM. Tritium 82-84 corticotropin releasing hormone Homo sapiens 0-30 1860838-1 1991 A peptide mitogen bombesin, which activates the phospholipase C-protein kinase C signaling pathway, induces a mepacrine-sensitive, dose-dependent increase in the release of [3H]arachidonic acid and its metabolites ([3H]AA) from prelabeled Swiss 3T3 fibroblasts. Tritium 174-176 gastrin releasing peptide Homo sapiens 18-26 1860838-1 1991 A peptide mitogen bombesin, which activates the phospholipase C-protein kinase C signaling pathway, induces a mepacrine-sensitive, dose-dependent increase in the release of [3H]arachidonic acid and its metabolites ([3H]AA) from prelabeled Swiss 3T3 fibroblasts. Tritium 216-218 gastrin releasing peptide Homo sapiens 18-26 1860838-3 1991 The initial transient [3H]AA liberation occurs concomitantly with bombesin-induced 45Ca efflux from prelabeled cells: both responses being substantially attenuated by loading cells with a Ca2+ chelator quin2. Tritium 23-25 gastrin releasing peptide Homo sapiens 66-74 7536611-4 1994 The relative potency of compounds for displacing [3H]-kainate binding to humEAA4 receptors expressed in COS or HEK-293 cells is domoate > kainate > quisqualate > 6-cyano-7-nitroquinoxaline-2,3-dione > L-glutamate = 6,7- dinitroquinoxaline-2,3-dione > dihydrokainate. Tritium 50-52 glutamate ionotropic receptor kainate type subunit 2 Homo sapiens 73-80 1860838-5 1991 The second sustained phase of the bombesin-induced [3H]AA release is abolished by reducing extracellular Ca2+ to 0.03 mM, although bombesin effects on phospholipase C and protein kinase C activation are barely affected by the same procedure. Tritium 52-54 gastrin releasing peptide Homo sapiens 34-42 1652050-5 1991 Expression of the human gene product in transfected cell lines results in the appearance of saturable high affinity 5-HT1D-type [3H]5-HT binding. Tritium 129-131 5-hydroxytryptamine receptor 1D Homo sapiens 116-122 8253851-5 1993 In addition, IGF-I or 10% FCS for 48 h increased the percentage of [3H]thymidine-labeled nuclei as compared to quiescent cells. Tritium 68-70 insulin-like growth factor 1 Rattus norvegicus 13-18 1944309-7 1991 In vitro incubation with NAD+ and 3H-labeled pregnenolone or dehydroepiandrosterone shows that the type I protein possesses a 3 beta HSD/delta 5-delta 4 isomerase activity higher than type II, with respective Km values of 0.24 vs. 1.2 microM for pregnenolone and 0.18 vs. 1.6 microM for dihydroepiandrosterone, while the specific activity of both types is equivalent. Tritium 34-36 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 Homo sapiens 126-136 8253851-6 1993 Single cell autoradiographic microphotographs show typical multilocular fat droplets brown adipocytes, resulting positive to [3H]thymidine-labeled nuclei in response to IGF-I. Tritium 126-128 insulin-like growth factor 1 Rattus norvegicus 169-174 1650214-9 1991 The Mz-Hep-1 Ah receptor sedimented in continuous sucrose gradients at approximately 9 S. Specificity of binding by [3H]TCDD was demonstrated by competitive binding of non-radiolabeled 2,3,7,8-tetrachlorodibenzofuran, 3-methylcholanthrene (MC), and dibenz[a,h]anthracene in 50-fold molar excess. Tritium 117-119 DNL-type zinc finger Homo sapiens 7-12 8274404-9 1993 Receptor binding assays confirmed that HO-FLU, Cypro.A and RU 23908-10 competed with [3H]DHT for AR binding with hAR expressed in CV-1 cells. Tritium 86-88 lymphatic vessel endothelial hyaluronan receptor 1 Homo sapiens 97-99 1649604-13 1991 Substrate-inactivated [Me-3H]methylated O6-MT was analysed by SDS/PAGE and electroblotting. Tritium 26-28 O-6-methylguanine-DNA methyltransferase Homo sapiens 40-45 1649604-16 1991 As expected, scintillation counting of the protein extracted from gel slices and linear-wire scanning of enzyme-associated radioactivity on electroblots were quicker methods for detecting the [Me-3H]methylated inactivated O6-MT. Tritium 196-198 O-6-methylguanine-DNA methyltransferase Homo sapiens 222-227 8274404-9 1993 Receptor binding assays confirmed that HO-FLU, Cypro.A and RU 23908-10 competed with [3H]DHT for AR binding with hAR expressed in CV-1 cells. Tritium 86-88 lymphatic vessel endothelial hyaluronan receptor 1 Homo sapiens 113-116 8227034-9 1993 Whereas PAF production seems extremely sensitive to changes in [Ca2+]i, which seems to exert its modulatory effect at the lyso-PAF:acetyl-CoA acetyltransferase step, [3H]arachidonate release seems tightly modulated by protein kinase C-dependent mechanisms and is coincidental with activation of phospholipase D. Tritium 167-170 PCNA clamp associated factor Homo sapiens 8-11 1721559-1 1991 3H-Labelled kappa-elastin peptides (kE:75 kDa molecular weight) were shown to bind to confluent human skin fibroblast (HSF) cultures in a time-dependent and saturable manner. Tritium 0-2 elastin Homo sapiens 18-25 1654152-8 1991 The ability of BN 50739 and RP 52770 to displace 3H PAF from its hypothalamic binding sites was correlated with their ability to alter basal hormone secretion and to counteract the PAF-stimulated secretion of HPA axis hormones in vivo (P less than 0.05 by ANOVA). Tritium 49-51 PCNA clamp associated factor Rattus norvegicus 52-55 7508812-0 1993 The phospholipase A2 inhibitor bromophenacyl bromide prevents the depolarization-induced increase in [3H]AMPA binding in rat brain synaptoneurosomes. Tritium 102-104 phospholipase A2 group IB Rattus norvegicus 4-20 1654152-8 1991 The ability of BN 50739 and RP 52770 to displace 3H PAF from its hypothalamic binding sites was correlated with their ability to alter basal hormone secretion and to counteract the PAF-stimulated secretion of HPA axis hormones in vivo (P less than 0.05 by ANOVA). Tritium 49-51 PCNA clamp associated factor Rattus norvegicus 181-184 1922009-3 1991 1986) (anti-PR monoclonal antibody that recognizes 110 kDa protein of chicken PR, subunit PR-B), which was seen to shift the rate of sedimentation of the untransformed (8S) and thermally transformed (4S) [3H]R5020-receptor complexes from the calf uterine cytosol toward the bottom of the tube. Tritium 205-207 RB transcriptional corepressor 1 Gallus gallus 90-94 2058685-3 1991 In the presence of 100 micrograms/ml bovine pituitary extract, both insulin-like growth factor (IGF) I (20 ng/ml) and II (25 ng/ml) further increased [3H]thymidine incorporation into pneumocyte DNA over that observed with the basal medium. Tritium 151-153 IGFI Bos taurus 68-102 7508812-3 1993 Treatment of intact synaptoneurosomes with the phospholipase A2 (PLA2) inhibitor, bromophenacyl bromide (BPB), produced a marked and dose-dependent reduction in KCl-induced enhancement in [3H]AMPA binding. Tritium 189-191 phospholipase A2 group IB Rattus norvegicus 47-63 7508812-3 1993 Treatment of intact synaptoneurosomes with the phospholipase A2 (PLA2) inhibitor, bromophenacyl bromide (BPB), produced a marked and dose-dependent reduction in KCl-induced enhancement in [3H]AMPA binding. Tritium 189-191 phospholipase A2 group IB Rattus norvegicus 65-69 1717064-6 1991 The time course of these effects was studied from 45 min to 8 h. In the presence of 24,25-(OH)2D3 (1 nM), delayed (3h) stimulation of PAL and GGT appeared. Tritium 115-117 leucine-rich repeat, Ig-like and transmembrane domains 1 Rattus norvegicus 134-137 8134310-4 1993 Homogenate binding studies, which used whole brain membranes, demonstrated that NPFF decreased the Bmax of mu binding sites (labeled by [3H][D-Ala2-MePhe4,Gly-ol5]enkephalin) from 262 +/- 12 to 192 +/- 12 fmolmg protein, and increased the Kd from 1.1 to 2.3 nM. Tritium 137-139 neuropeptide FF-amide peptide precursor Rattus norvegicus 80-84 1788558-1 1991 The inhibitory effect of dexamethasone (Dex) on the incorporation of [3H] Urd and specific binding of [3H] Dex with glucocorticoid receptor (GR) were measured in order to quantitatively study the relationship between these two indices in rat thymocytes. Tritium 103-105 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 116-139 8134310-4 1993 Homogenate binding studies, which used whole brain membranes, demonstrated that NPFF decreased the Bmax of mu binding sites (labeled by [3H][D-Ala2-MePhe4,Gly-ol5]enkephalin) from 262 +/- 12 to 192 +/- 12 fmolmg protein, and increased the Kd from 1.1 to 2.3 nM. Tritium 137-139 proenkephalin Rattus norvegicus 163-173 1896966-4 1991 The incorporation of both [35S]sulfate and [3H]glucosamine into the GAG was decreased by thrombin, while the ratio of 35S to 3H was not changed. Tritium 44-46 coagulation factor II, thrombin Bos taurus 89-97 8134310-6 1993 The density of mu opioid binding sites labeled by [3H][D-Ala2-MePhe4,Gly-ol5]enkephalin was decreased in all brain areas measured except the corpus callosum, and there was no change in dynorphin mRNA or enkephalin mRNA in the caudate, the nucleus accumbens, or the ventral pallidum. Tritium 51-53 proenkephalin Rattus norvegicus 77-87 7907269-1 1993 PBMC proliferation in patients with SLE was assessed by incorporation of 3H-Tdr in an accessory cell-dependent response to anti-CD2 specific monoclonal antibody. Tritium 73-75 CD2 molecule Homo sapiens 128-131 2040661-6 1991 Other mitogenic agents, 12-0-tetradecanoyl phorbol 13-acetate (TPA) and exogenous phospholipase C, also increased BMM levels of [3H]myristoyl- and [3H]arachidonyl-DAG. Tritium 129-131 lymphocyte antigen 6 complex Mus musculus 163-166 2023913-7 1991 Chemical crosslinking of [3H]AVP complex, SDS/PAGE of the products, and analysis of gel slices by scintillation counting led to detection of two major radiolabeled bands of 31 and 38 kDa. Tritium 26-28 arginine vasopressin Bos taurus 29-32 8409903-7 1993 Bovine insulin (10 micrograms/ml) and human recombinant insulin-like growth factor-I (IGF-I, 15 ng/ml) also doubled [3H]thymidine uptake in PrM cultures, but lower doses were less effective and estradiol-17 beta, transferrin, adult shark serum, purified shark relaxin, and a variety of other known growth factors were neither stimulatory nor inhibitory at the doses and conditions tested. Tritium 117-119 insulin Bos taurus 7-14 1826282-9 1991 In vitro, [3H]thymidine incorporation into hepatocyte DNA in the presence of hepatocyte growth factor is greater if 10(-5) mol/L norepinephrine is also present in the media. Tritium 11-13 hepatocyte growth factor Rattus norvegicus 77-101 7902963-1 1993 Neurotensin (NT) (10(-8)-10(-6)) exerted a dose-dependent increase in the tone and release of [3H]ACh in the guinea-pig gallbladder muscle strips but was inefficient in the canine gallbladder muscle strips. Tritium 95-97 neurotensin/neuromedin N Cavia porcellus 0-11 1862748-3 1991 In addition, we have utilised the loss of the relatively specific labelling of glyceraldehyde-3-phosphate dehydrogenase (G3PDH) by [3H]-iodoacetic acid to show that rabbit articular G3PDH is oxidised in vivo during the animal model of acute arthritis, carrageenin-induced arthritis, in the same way as we have previously shown that cartilage G3PDH is oxidised after in vitro exposure to sublethal doses of H2O2. Tritium 132-134 glyceraldehyde-3-phosphate dehydrogenase Oryctolagus cuniculus 79-119 1862748-3 1991 In addition, we have utilised the loss of the relatively specific labelling of glyceraldehyde-3-phosphate dehydrogenase (G3PDH) by [3H]-iodoacetic acid to show that rabbit articular G3PDH is oxidised in vivo during the animal model of acute arthritis, carrageenin-induced arthritis, in the same way as we have previously shown that cartilage G3PDH is oxidised after in vitro exposure to sublethal doses of H2O2. Tritium 132-134 glyceraldehyde-3-phosphate dehydrogenase Oryctolagus cuniculus 121-126 1997179-8 1991 In cells depleted to the extent of 90-95% of their ATP by 60-min incubation in medium in the absence of D-glucose and with 10 mM sodium azide, overall efflux of [3H]MTX was markedly reduced and appears to be mediated solely by the MTX influx route. Tritium 162-164 metaxin 1 Mus musculus 165-168 1997179-9 1991 Influx of [3H]MTX was both cis and trans inhibited by probenecid, and efflux under these conditions was markedly inhibited by the N-hydroxysuccinimide ester of MTX and trans-stimulated by MTX. Tritium 11-13 metaxin 1 Mus musculus 14-17 8239509-2 1993 Our results demonstrate that c-myc and max genes are differentially expressed in HBC cells which was accompanied by increases in [3H]-thymidine incorporation and percent of cells in the S phase of cell cycle. Tritium 130-132 MYC proto-oncogene, bHLH transcription factor Homo sapiens 29-34 1997179-9 1991 Influx of [3H]MTX was both cis and trans inhibited by probenecid, and efflux under these conditions was markedly inhibited by the N-hydroxysuccinimide ester of MTX and trans-stimulated by MTX. Tritium 11-13 metaxin 1 Mus musculus 160-163 1997179-9 1991 Influx of [3H]MTX was both cis and trans inhibited by probenecid, and efflux under these conditions was markedly inhibited by the N-hydroxysuccinimide ester of MTX and trans-stimulated by MTX. Tritium 11-13 metaxin 1 Mus musculus 160-163 8364019-9 1993 The increase in [3H]cholesterol esterification with MP medium in the presence of beta-VLDL was also elicited in cells obtained from LDL receptor-negative donors with familial hypercholesterolemia (FH-HSF), even though in these cells significantly less [3H]cholesteryl ester was formed in the presence of beta-VLDL. Tritium 17-19 low density lipoprotein receptor Homo sapiens 132-144 1847855-6 1991 FRTL BAR had significantly higher levels of intracellular cAMP, [3H]thymidine incorporation, and iodide uptake in the absence of added isoproterenol than FRTL RBAR or wild-type cells. Tritium 65-67 adrenoceptor beta 2 Homo sapiens 5-8 1847855-9 1991 Isoproterenol enhanced the ability of IGF-I to stimulate [3H]thymidine incorporation in BAR but not RBAR cells. Tritium 58-60 adrenoceptor beta 2 Homo sapiens 88-91 1847384-5 1991 Scatchard analysis in both cell lines revealed one class of specific binding sites for [3H] flunitrazepam verified as being MBR by displacement studies with a series of MBR ligands. Tritium 88-90 translocator protein Homo sapiens 124-127 1847384-5 1991 Scatchard analysis in both cell lines revealed one class of specific binding sites for [3H] flunitrazepam verified as being MBR by displacement studies with a series of MBR ligands. Tritium 88-90 translocator protein Homo sapiens 169-172 1996987-3 1991 The presence of 2-10 mM dithiothreitol (DTT), beta-mercaptoethanol (beta-MER), and monothioglycerol (MTG) caused a 2-3 fold increase in the [3H]TA and [3H]RU486 binding to GR. Tritium 141-143 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 172-174 1996987-5 1991 In contrast, the [3H]RU486 binding to GR increased by 50 percent in the presence of IA. Tritium 18-20 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 38-40 8396012-8 1993 This was paralleled by a loss of [3H]OCT associated with the intestinal vitamin D receptor. Tritium 34-36 vitamin D receptor Rattus norvegicus 72-90 8376601-2 1993 EGF induced a 10-fold increase in DNA synthesis as assessed by [3H]thymidine incorporation in cultured hepatocytes. Tritium 64-66 epidermal growth factor Rattus norvegicus 0-3 1702633-4 1991 Pretreatment with the 5-HT2/5-HT1C antagonist, ketanserin, inhibited the MDMA-induced decrease in 5-HT and 5-HIAA concentrations and the number of [3H]paroxetine binding sites in the frontal cortex 7 days following a single administration. Tritium 148-150 5-hydroxytryptamine receptor 2C Rattus norvegicus 28-34 1835629-4 1991 We compared murine albumin, vitamin D binding protein and B700 for their ability to specifically bind [3H]-1,25-dihydroxy-vitamin D3. Tritium 103-105 vitamin D binding protein Mus musculus 28-53 8395567-7 1993 Of the membrane-associated [3H][D-Ala2,N-Me-Phe4,Gly5-ol]-enkephalin binding sites, 43 +/- 3% were recovered in the solubilized material. Tritium 28-30 proenkephalin Rattus norvegicus 58-68 12106250-9 1991 Although > 50% of the [3H]ArA was metabolized to PGF2alpha, PGE2, PGD2, HHT, 15- and 11-HETE in cultured astrocyte homogenates, no [3H]ArA metabolism could be detected in cultured striatal neuron homogenates. Tritium 26-28 prostaglandin D2 synthase (brain) Mus musculus 69-73 8360670-4 1993 Using cells cultured in the absence of FBS, epidermal growth factor (EGF) and fibroblast growth factor also stimulated production of [3H]Ins(1,3,4)P3, [3H]Ins(1,4,5)P3, and [3H]Ins(1,3,4,5)P4. Tritium 134-136 epidermal growth factor Rattus norvegicus 44-73 1783409-4 1991 Using a [3H]-labeled probe derived from a hGK-1 genomic clone, we demonstrated hybridization confined to the q13.3 and q13.4 bands of chromosome 19 and suggest that kallikrein genes may possibly be located near the border of these two bands. Tritium 9-11 kallikrein related peptidase 2 Homo sapiens 42-47 1783409-4 1991 Using a [3H]-labeled probe derived from a hGK-1 genomic clone, we demonstrated hybridization confined to the q13.3 and q13.4 bands of chromosome 19 and suggest that kallikrein genes may possibly be located near the border of these two bands. Tritium 9-11 kallikrein related peptidase 4 Homo sapiens 165-175 8360670-4 1993 Using cells cultured in the absence of FBS, epidermal growth factor (EGF) and fibroblast growth factor also stimulated production of [3H]Ins(1,3,4)P3, [3H]Ins(1,4,5)P3, and [3H]Ins(1,3,4,5)P4. Tritium 152-154 epidermal growth factor Rattus norvegicus 44-73 8360670-4 1993 Using cells cultured in the absence of FBS, epidermal growth factor (EGF) and fibroblast growth factor also stimulated production of [3H]Ins(1,3,4)P3, [3H]Ins(1,4,5)P3, and [3H]Ins(1,3,4,5)P4. Tritium 152-154 epidermal growth factor Rattus norvegicus 44-73 8219035-2 1993 The overflow of [3H]serotonin elicited by high potassium (30 mM) in superfused slices of rat hippocampus was significantly inhibited in the presence of two agonists, mu-selective [D-Ala2,N-methyl-Phe4,Gly5ol]enkephalin (DAGO) and delta-selective [D-Pen2,D-Pen5]enkephalin (DPDPE) in control animals. Tritium 17-19 proenkephalin Rattus norvegicus 208-218 1794447-2 1991 Uptake of 3H-25OHD3, 3H-25OHD3-DBP, 125I-holo-DBP and 125I-apo-DBP by LLC-PK1 cells was linearly related to the concentration of each in the culture media. Tritium 21-23 GC vitamin D binding protein Sus scrofa 31-34 1794447-3 1991 The presence of DBP in the medium significantly reduced the amount of 3H-25OHD3 taken up by cells. Tritium 70-72 GC vitamin D binding protein Sus scrofa 16-19 8219035-2 1993 The overflow of [3H]serotonin elicited by high potassium (30 mM) in superfused slices of rat hippocampus was significantly inhibited in the presence of two agonists, mu-selective [D-Ala2,N-methyl-Phe4,Gly5ol]enkephalin (DAGO) and delta-selective [D-Pen2,D-Pen5]enkephalin (DPDPE) in control animals. Tritium 17-19 proenkephalin Rattus norvegicus 261-271 8284266-5 1993 Mouse brain membranes were depleted of mu sites by pretreatment with the site-directed acylating agent, BIT, and the delta ncx binding sites were labeled with [3H][D-Ala2,D-Leu5]enkephalin. Tritium 160-162 T cell leukemia, homeobox 2 Mus musculus 123-126 1851141-2 1991 Considering the presence of specific receptors on the surface of spleen cells, the effect of 2.5s nerve growth factor on 3H-thymidine uptake, cAMP and cGMP accumulation in mouse spleen lymphocytes has been studied. Tritium 121-123 nerve growth factor Mus musculus 98-117 8217815-6 1993 After 48 h of mitogen stimulation there was a 4-10-fold increase in FPGS mRNA and folypolyglutamate formation (Glu > or = 5) was essentially simultaneous with 5CH3[3H]FH4 transport. Tritium 167-169 folylpolyglutamate synthase Homo sapiens 68-72 2016261-8 1991 High-performance ion-exchange liquid chromatography analysis showed that the transformed androgen receptor bound to [3H]R1881 with high affinity. Tritium 117-119 androgen receptor Rattus norvegicus 89-106 2016261-10 1991 In addition, the hsp90-free androgen receptor prebound with radioinert R1881 was efficiently relabeled with [3H]R1881, while the triamcinolone acetonide-bound, transformed glucocorticoid receptor failed in ligand exchange. Tritium 109-111 androgen receptor Rattus norvegicus 28-45 8401931-2 1993 [3H]-5-hydroxytryptamine (5-HT) has been shown to radiolabel at least five types of 5-HT binding sites in mammalian brain tissue, 5-HT1A, 5-HT1C, 5-HT1D and 5-HT1D and 5-HT1E (Frazer et al., 1990). Tritium 1-3 5-hydroxytryptamine receptor 1A Homo sapiens 130-136 1987318-5 1991 The asymmetrical loss of the dopamine transporter in Parkinson"s diseased striatum was confirmed in reversible receptor binding experiments using [3H]GBR-12935 (3H-labeled 1-[2-(diphenylmethoxy) ethyl]-4-(3-phenylpropyl)piperazine). Tritium 147-149 solute carrier family 6 member 3 Homo sapiens 29-49 1987318-5 1991 The asymmetrical loss of the dopamine transporter in Parkinson"s diseased striatum was confirmed in reversible receptor binding experiments using [3H]GBR-12935 (3H-labeled 1-[2-(diphenylmethoxy) ethyl]-4-(3-phenylpropyl)piperazine). Tritium 161-163 solute carrier family 6 member 3 Homo sapiens 29-49 1766257-5 1991 With IL-2, 3H-TdR incorporation increased in cells from 5 out of 10 T-ALL patients, including those with CD7+4-8-, CD5+, and CD1+ acute leukemia. Tritium 11-13 CD7 molecule Homo sapiens 105-108 1766257-6 1991 In the presence of IL-3 or IL-6, 3H-TdR incorporation increased in cells from 2 out of 5 patients with CD7+4-8- acute leukemia. Tritium 33-35 CD7 molecule Homo sapiens 103-106 1766257-8 1991 With IL-4, 3H-TdR incorporation was increased in the cells from 2 out of 5 patients with CD7+4-8- acute leukemia and in the cells of 2 of those with CD1+ acute leukemia. Tritium 11-13 CD7 molecule Homo sapiens 89-92 1766257-9 1991 IL-5 increased the 3H-TdR incorporation by cells from 2 out of 5 patients with CD7+4-8- acute leukemia and 1 patient with CD1+ acute leukemia. Tritium 19-21 CD7 molecule Homo sapiens 79-82 1847230-5 1991 [3H]PK 14105 photolabeled the bovine PBR and the Mr was estimated under nondenaturing (200 kDa) and denaturing (17 kDa) conditions. Tritium 1-3 translocator protein Bos taurus 37-40 20504708-2 1991 [(3)H]8-OH-DPAT, [(125)I]ICYP, [(3)H]mesulergine and [(3)H]ketanserin are radiolabeled ligands for 5-HT(1A), 5-HT(1B), 5-HT(1C) and 5-HT(2) sites, respectively. Tritium 0-6 5-hydroxytryptamine receptor 1B Rattus norvegicus 109-116 20504708-2 1991 [(3)H]8-OH-DPAT, [(125)I]ICYP, [(3)H]mesulergine and [(3)H]ketanserin are radiolabeled ligands for 5-HT(1A), 5-HT(1B), 5-HT(1C) and 5-HT(2) sites, respectively. Tritium 0-6 5-hydroxytryptamine receptor 2C Rattus norvegicus 119-126 8376633-7 1993 Both heated and native lactoferrins enhanced [3H]thymidine incorporation by U937 cells, except for Fe-lactoferrin heated at 85 degrees C for 20 min, which was inhibitory. Tritium 46-48 lactotransferrin Bos taurus 23-34 1701964-2 1990 Pancreatic generation of PAF, as measured by bioassay (ie, platelet [3H]serotonin secretion), was determined at various times after induction of inflammation. Tritium 69-71 PCNA clamp associated factor Rattus norvegicus 25-28 1701964-7 1990 Long-term in vivo administration of exogenous PAF (20 micrograms/kg/hr for 7 days) exhibited a reduction of [3H]thymidine uptake into and amylase release from pancreatic acini in vitro. Tritium 109-111 PCNA clamp associated factor Rattus norvegicus 46-49 8394771-4 1993 The results showed an age-related decrease in the density of [3H]-KA binding in several regions of the hippocampal formation (CA3, CA1, hilus) and within related cortical areas (subicular complex, entorhinal cortex, perirhinal cortex). Tritium 62-64 carbonic anhydrase 1 Rattus norvegicus 131-134 1703543-3 1990 These antibodies immunoprecipitated [3H]hyaluronate binding activity from detergent extracts of both mouse and human cells, indicating that the hyaluronate receptor is identical to CD44. Tritium 37-39 CD44 molecule (Indian blood group) Homo sapiens 144-164 8505227-4 1993 The maximum measured tritium concentration at Highway 301 (a major sampling point 37 km downstream of the Savannah River Site) was 2.5 Bq mL-1. Tritium 21-28 L1 cell adhesion molecule Mus musculus 138-142 8505228-4 1993 Dose was estimated assuming a concentration of tritium in atmospheric moisture of 0.13 +/- 0.16 Bq mL-1. Tritium 47-54 L1 cell adhesion molecule Mus musculus 99-103 8410799-8 1993 When 25 g albumin l-1 was added, 22% of [3H]PAF was hydrolysed h-1 compared with 72% in media without albumin. Tritium 41-43 PCNA clamp associated factor Homo sapiens 44-47 2262901-3 1990 We now report that these monoclonal antibodies can induce the net efflux of [3H]-T-2 toxin from poisoned human B-lymphoblastoid cells in vitro, and restore protein synthesis. Tritium 77-79 solute carrier family 25 member 5 Homo sapiens 81-84 8393519-9 1993 The delta subtype receptors that bind [3H][D-Pen2,D-Pen5]enkephalin in neonatal or adult brain membranes were unaffected by 0.5-2.5 mg/kg buprenorphine treatment. Tritium 39-42 proenkephalin Rattus norvegicus 57-67 1979919-9 1990 With polyclonal antibodies raised against aminopeptidase N from pig renal microsomes the aminopeptidase N from solubilized brush border membranes from pig small intestine could be completely precipitated; the binding protein for beta-lactam antibiotics and oligopeptides of apparent Mr 127,000 identified by direct photoaffinity labeling with [3H]benzylpenicillin showed no crossreactivity with the aminopeptidase N anti serum and was not precipitated by the anti serum. Tritium 344-346 alanyl aminopeptidase, membrane Sus scrofa 89-105 1979919-9 1990 With polyclonal antibodies raised against aminopeptidase N from pig renal microsomes the aminopeptidase N from solubilized brush border membranes from pig small intestine could be completely precipitated; the binding protein for beta-lactam antibiotics and oligopeptides of apparent Mr 127,000 identified by direct photoaffinity labeling with [3H]benzylpenicillin showed no crossreactivity with the aminopeptidase N anti serum and was not precipitated by the anti serum. Tritium 344-346 alanyl aminopeptidase, membrane Sus scrofa 89-105 8323562-2 1993 CTLL cultures in log phase and growing in 10% IL-2 medium in the presence of 1-16 micrograms vitamin E/ml showed a dose-dependent 2- to 15-fold stimulation of growth (assessed by 3H-thymidine incorporation) relative to controls. Tritium 179-181 interleukin 2 Mus musculus 46-50 1976513-3 1990 PKC levels were monitored by [3H]PDBU binding assay and by using an anti-PKC antibody that detected intact PKC (79 kd) as well as its catalytic and regulatory domains. Tritium 30-32 protein kinase C, gamma Rattus norvegicus 0-3 1976513-4 1990 The [3H]PDBU binding to the membrane-associated PKC increased within 15-30 min of TPA treatment; thereafter total cellular [3H]PDBU binding decreased to a minimum of 20% of the control at 8 h. The rate of decrease in binding was greater than the decrease in the intensity of the staining of PKC holo enzyme visualized by anti-PKC antibody. Tritium 5-7 protein kinase C, gamma Rattus norvegicus 48-51 8394594-4 1993 Addition of NMB has a biphasic effect on [3H]thymidine ([3H]dT) incorporation in confluent and quiescent cells: up to 10 nM of NMB causes a 1.5-3-fold stimulation of [3H]dT incorporation, but at greater than 10 nM there is inhibition of [3H]dT incorporation, and at 100 nM of NMB there is inhibition of cell growth. Tritium 42-44 neuromedin B Mus musculus 12-15 2173726-4 1990 ANP (10(-7) M) significantly suppressed the proliferative effect of 1% and 5% serum as measured by 3H-thymidine incorporation and cell number, confirming ANP as an antimitogenic factor. Tritium 99-101 natriuretic peptide A Rattus norvegicus 0-3 8394594-4 1993 Addition of NMB has a biphasic effect on [3H]thymidine ([3H]dT) incorporation in confluent and quiescent cells: up to 10 nM of NMB causes a 1.5-3-fold stimulation of [3H]dT incorporation, but at greater than 10 nM there is inhibition of [3H]dT incorporation, and at 100 nM of NMB there is inhibition of cell growth. Tritium 42-44 neuromedin B Mus musculus 127-130 8394594-4 1993 Addition of NMB has a biphasic effect on [3H]thymidine ([3H]dT) incorporation in confluent and quiescent cells: up to 10 nM of NMB causes a 1.5-3-fold stimulation of [3H]dT incorporation, but at greater than 10 nM there is inhibition of [3H]dT incorporation, and at 100 nM of NMB there is inhibition of cell growth. Tritium 42-44 neuromedin B Mus musculus 127-130 8394594-4 1993 Addition of NMB has a biphasic effect on [3H]thymidine ([3H]dT) incorporation in confluent and quiescent cells: up to 10 nM of NMB causes a 1.5-3-fold stimulation of [3H]dT incorporation, but at greater than 10 nM there is inhibition of [3H]dT incorporation, and at 100 nM of NMB there is inhibition of cell growth. Tritium 57-59 neuromedin B Mus musculus 12-15 2120386-7 1990 Pharmacological characterization suggests that [3H]WIN 35,065-2 binds to the dopamine transporter. Tritium 48-50 solute carrier family 6 member 3 Homo sapiens 77-97 8394594-4 1993 Addition of NMB has a biphasic effect on [3H]thymidine ([3H]dT) incorporation in confluent and quiescent cells: up to 10 nM of NMB causes a 1.5-3-fold stimulation of [3H]dT incorporation, but at greater than 10 nM there is inhibition of [3H]dT incorporation, and at 100 nM of NMB there is inhibition of cell growth. Tritium 57-59 neuromedin B Mus musculus 127-130 1706443-3 1990 Rapid fluorochromasia assay with propidium iodide (RFP) was used for the quantitative measurement of LPT instead of 3H-thymidine uptake. Tritium 116-118 tripartite motif-containing 27 Mus musculus 51-54 1981163-2 1990 In order to investigate neurotensin-dopamine receptor interactions in vivo, the effects of intraventricular injection of neurotensin were analyzed on S(-)[N-propyl-3H(N)]propylnorapomorphine [( 3H]NPA) binding in cryostat sections of the forebrain, hypothalamus and pituitary gland, and on serum levels of prolactin, luteinizing hormone and corticosterone in the male rat. Tritium 164-166 neurotensin Rattus norvegicus 121-132 1981163-3 1990 The relationship of modulation of [3H]NPA binding with neurotensin-dopamine coexistence in nerve terminals was analyzed by investigating coexistence of neurotensin and tyrosine hydroxylase (TH) immunoreactive nerve terminals in various brain areas, using a double immunohistofluorescence procedure. Tritium 35-37 neurotensin Rattus norvegicus 55-66 1981163-4 1990 Intraventricular injections of neurotensin (0.03-3 nmol, 30 min) reduced dose-dependently specific [3H]NPA binding (0.25 nM) in the caudate-putamen (-38 +/- 4%), nucleus accumbens (-42 +/- 5%), tuberculum olfactorium (-52 +/- 7%) and in the intermediate lobe of the pituitary gland (-17 +/- 2%). Tritium 100-102 neurotensin Rattus norvegicus 31-42 8394594-4 1993 Addition of NMB has a biphasic effect on [3H]thymidine ([3H]dT) incorporation in confluent and quiescent cells: up to 10 nM of NMB causes a 1.5-3-fold stimulation of [3H]dT incorporation, but at greater than 10 nM there is inhibition of [3H]dT incorporation, and at 100 nM of NMB there is inhibition of cell growth. Tritium 57-59 neuromedin B Mus musculus 127-130 8389343-5 1993 Aliquot portions were incubated with 3H-PGE2 or 3H-PGF2 alpha at 37 degrees C for 30 min in a final volume of 500 microliters. Tritium 48-50 prostaglandin F synthase 2 Bos taurus 51-55 1699431-2 1990 CCK-8 caused an increase in [3H]IP2 and [3H]IP3 at 10 s and a slower increase in [3H]IP1. Tritium 28-31 cholecystokinin Rattus norvegicus 0-3 1699431-2 1990 CCK-8 caused an increase in [3H]IP2 and [3H]IP3 at 10 s and a slower increase in [3H]IP1. Tritium 40-43 cholecystokinin Rattus norvegicus 0-3 1699431-2 1990 CCK-8 caused an increase in [3H]IP2 and [3H]IP3 at 10 s and a slower increase in [3H]IP1. Tritium 29-31 cholecystokinin Rattus norvegicus 0-3 8389343-13 1993 The rank order of potency for agonist displacement by both 3H-PGE2 and 3H-PGF2 in sphincter muscle membrane was PGE2 > PGF2 alpha > PGD2 > Iloprost. Tritium 59-61 prostaglandin F synthase 2 Bos taurus 122-126 7684381-5 1993 Deletion or mutation of Cys766 eliminated [3H] palmitic acid labeling of P-selectin in transfected COS-7 cells. Tritium 43-45 selectin P Homo sapiens 73-83 2168937-1 1990 Previous study has shown that cholecystokinin (CCK) octapeptide (CCK-8) suppressed the binding of opioid receptors to the universal opioid agonist [3H]etorphine. Tritium 148-150 cholecystokinin Rattus norvegicus 30-45 2168937-1 1990 Previous study has shown that cholecystokinin (CCK) octapeptide (CCK-8) suppressed the binding of opioid receptors to the universal opioid agonist [3H]etorphine. Tritium 148-150 cholecystokinin Rattus norvegicus 47-50 2168937-1 1990 Previous study has shown that cholecystokinin (CCK) octapeptide (CCK-8) suppressed the binding of opioid receptors to the universal opioid agonist [3H]etorphine. Tritium 148-150 cholecystokinin Rattus norvegicus 65-68 2168937-3 1990 In the competition experiments, CCK-8 suppressed the binding of [3H]DAGO and [3H]U69,593 but not that of [3H]DPDPE to the respective opioid receptor. Tritium 65-67 cholecystokinin Rattus norvegicus 32-35 2168937-3 1990 In the competition experiments, CCK-8 suppressed the binding of [3H]DAGO and [3H]U69,593 but not that of [3H]DPDPE to the respective opioid receptor. Tritium 78-80 cholecystokinin Rattus norvegicus 32-35 2168937-3 1990 In the competition experiments, CCK-8 suppressed the binding of [3H]DAGO and [3H]U69,593 but not that of [3H]DPDPE to the respective opioid receptor. Tritium 78-80 cholecystokinin Rattus norvegicus 32-35 8512589-7 1993 Increased [3H]DEP uptake was accompanied by increased platination of DNA (8.5-fold in 2008 cells and 18.5-fold in 2008/C13*5.25 cells), and by enhanced killing of both the DDP-sensitive and -resistant cells that was shown to be synergistic by median effect analysis. Tritium 11-13 translocase of inner mitochondrial membrane 8A Homo sapiens 172-175 2168937-5 1990 In the saturation experiments, CCK-8 at concentrations of 0.1 nmol/L to 1 mumol/L decreased the Bmax of [3H]DAGO binding sites without affecting the KD; on the other hand, CCK-8 increased the KD of [3H]U69,593 binding without changing the Bmax. Tritium 105-107 cholecystokinin Rattus norvegicus 31-34 2168937-5 1990 In the saturation experiments, CCK-8 at concentrations of 0.1 nmol/L to 1 mumol/L decreased the Bmax of [3H]DAGO binding sites without affecting the KD; on the other hand, CCK-8 increased the KD of [3H]U69,593 binding without changing the Bmax. Tritium 105-107 cholecystokinin Rattus norvegicus 172-175 2168937-5 1990 In the saturation experiments, CCK-8 at concentrations of 0.1 nmol/L to 1 mumol/L decreased the Bmax of [3H]DAGO binding sites without affecting the KD; on the other hand, CCK-8 increased the KD of [3H]U69,593 binding without changing the Bmax. Tritium 199-201 cholecystokinin Rattus norvegicus 31-34 2168937-5 1990 In the saturation experiments, CCK-8 at concentrations of 0.1 nmol/L to 1 mumol/L decreased the Bmax of [3H]DAGO binding sites without affecting the KD; on the other hand, CCK-8 increased the KD of [3H]U69,593 binding without changing the Bmax. Tritium 199-201 cholecystokinin Rattus norvegicus 172-175 8512589-9 1993 We conclude that a brief exposure to digitonin can increase [3H]DEP uptake in vitro, and can overcome the impaired [3H]DEP accumulation associated with acquired DDP resistance. Tritium 116-118 translocase of inner mitochondrial membrane 8A Homo sapiens 161-164 8503848-5 1993 Most of the newly synthesized [3H]PAF formed in response to acrosome reaction was found in the supernatant, suggesting a release of the phospholipid from spermatozoa. Tritium 31-33 PCNA clamp associated factor Homo sapiens 34-37 2282454-12 1990 When the single pass transpulmonary binding of 80pmol [3H]-RAC-X-65 (an ACE inhibitor) was measured in additional rabbits, a significant (P < 0.05) decrease in RAC-X-65 binding was observed 30s after captopril (80% decrease) or CL242,817 (85% decrease), a result expected for a loss of catalytically active enzyme mass due to tightly bound captopril or CL242,817. Tritium 55-57 angiotensin-converting enzyme Oryctolagus cuniculus 72-75 8482722-6 1993 Exogenous addition of IGF-II increased 3H-TdR incorporation and cell number in a dose- and time-dependent fashion. Tritium 39-41 insulin like growth factor 2 Homo sapiens 22-28 2168484-9 1990 On the nACh-R, spermine, spermidine and agmatine inhibited [125I]alpha-bungarotoxin and also [3H]H12-HTX binding in presence of carbamylcholine. Tritium 94-96 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 7-13 8476056-4 1993 Both CCK-8 and gastrin increased inositol phosphates, with CCK-8 (1 microM) and gastrin (3 microM) causing a 40- and 14-fold increase in [3H]IP1, 10- and 6-fold for [3H]IP2, and 8- and 4-fold for [3H]IP3. Tritium 138-140 gastrin Homo sapiens 15-22 2259456-5 1990 IDPN caused significant increases of 5-[3H]HT binding in the oriens and pyramidal layers of the CA3 field of hippocampus. Tritium 40-42 carbonic anhydrase 3 Rattus norvegicus 96-99 8476056-4 1993 Both CCK-8 and gastrin increased inositol phosphates, with CCK-8 (1 microM) and gastrin (3 microM) causing a 40- and 14-fold increase in [3H]IP1, 10- and 6-fold for [3H]IP2, and 8- and 4-fold for [3H]IP3. Tritium 138-140 gastrin Homo sapiens 80-87 1976403-0 1990 Identification of human platelet alpha 2-adrenoceptors with a new antagonist [3H]-RX821002, a 2-methoxy derivative of idazoxan. Tritium 78-80 adrenoceptor alpha 2A Homo sapiens 33-54 1976403-7 1990 The difference in total binding is due to a better labelling of the alpha 2-adrenoceptors in the low affinity state by [3H]-RX821002 since the labelled receptors number in high affinity state was identical with the two radioligands. Tritium 120-122 adrenoceptor alpha 2A Homo sapiens 68-89 1976403-15 1990 These results indicate that [3H]-RX821002 is a suitable ligand for the identification of human platelet alpha 2-adrenoceptors. Tritium 29-31 adrenoceptor alpha 2A Homo sapiens 104-125 2207505-23 1990 GABA also inhibited [3H]-IP1 formation induced by endothelin-1 in guinea-pig cerebellar slices and increased, but not significantly, the amount of [3H]-IP2 accumulated. Tritium 21-23 endothelin-1 Cavia porcellus 50-62 8476056-4 1993 Both CCK-8 and gastrin increased inositol phosphates, with CCK-8 (1 microM) and gastrin (3 microM) causing a 40- and 14-fold increase in [3H]IP1, 10- and 6-fold for [3H]IP2, and 8- and 4-fold for [3H]IP3. Tritium 166-168 gastrin Homo sapiens 15-22 2164924-7 1990 GR capacity was determined with [3H]RU 28362 (Kd = 0.39 nM, Bmax = 120 fmol/mg). Tritium 33-35 nuclear receptor subfamily 3 group C member 1 Canis lupus familiaris 0-2 8476056-4 1993 Both CCK-8 and gastrin increased inositol phosphates, with CCK-8 (1 microM) and gastrin (3 microM) causing a 40- and 14-fold increase in [3H]IP1, 10- and 6-fold for [3H]IP2, and 8- and 4-fold for [3H]IP3. Tritium 166-168 gastrin Homo sapiens 80-87 8476056-4 1993 Both CCK-8 and gastrin increased inositol phosphates, with CCK-8 (1 microM) and gastrin (3 microM) causing a 40- and 14-fold increase in [3H]IP1, 10- and 6-fold for [3H]IP2, and 8- and 4-fold for [3H]IP3. Tritium 166-168 gastrin Homo sapiens 15-22 8476056-4 1993 Both CCK-8 and gastrin increased inositol phosphates, with CCK-8 (1 microM) and gastrin (3 microM) causing a 40- and 14-fold increase in [3H]IP1, 10- and 6-fold for [3H]IP2, and 8- and 4-fold for [3H]IP3. Tritium 166-168 gastrin Homo sapiens 80-87 2166682-5 1990 Ins 1,3,4,5,6-P5 displaced [3H]Ins 1,3,4,5-P4 binding with a comparable affinity. Tritium 28-30 forkhead box M1 Homo sapiens 0-5 2166682-5 1990 Ins 1,3,4,5,6-P5 displaced [3H]Ins 1,3,4,5-P4 binding with a comparable affinity. Tritium 28-30 forkhead box M1 Homo sapiens 31-36 7681399-5 1993 Furthermore, L-659,989 and PAF inhibited specific 3H-labeled PAF binding in TNF-treated, but not in untreated cells. Tritium 50-52 PCNA clamp associated factor Homo sapiens 27-30 2164068-9 1990 Exposure of [3H]choline-labeled macrophages to IFN-gamma resulted in an increase in the basal level of aqueous [3H]choline metabolites as well as potentiating the production of [3H]choline in response to PAF, PMA, and ionomycin. Tritium 13-15 patchy fur Mus musculus 204-207 7681399-5 1993 Furthermore, L-659,989 and PAF inhibited specific 3H-labeled PAF binding in TNF-treated, but not in untreated cells. Tritium 50-52 PCNA clamp associated factor Homo sapiens 61-64 8097600-6 1993 The P450 inhibitor 7,8-benzoflavone (10(-6) M) partially prevented BaP inhibition of [3H]TdR incorporation. Tritium 86-88 prohibitin 2 Homo sapiens 67-70 2370805-1 1990 The pharmacokinetic parameters of muscarinic cholinergic drugs after intravenous (IV) and oral administration to mice was determined with ex vivo (3H)-oxotremorine-M (3H-Oxt) binding to the brain. Tritium 167-169 oxytocin Mus musculus 170-173 8097600-10 1993 Despite the BaP-mediated decrease in [3H]TdR incorporation, cell-cycle analysis indicated that BaP at 10(-7) and 10(-6) M increased the percentages of cells in S phase, and correspondingly decreased the percentages of cells in the G0/G1 phase. Tritium 38-40 prohibitin 2 Homo sapiens 12-15 2370805-7 1990 These results indicate that the pharmacokinetic profile of muscarinic cholinergic drugs can be determined with ex vivo (3H)-Oxt binding. Tritium 120-122 oxytocin Mus musculus 124-127 8097600-10 1993 Despite the BaP-mediated decrease in [3H]TdR incorporation, cell-cycle analysis indicated that BaP at 10(-7) and 10(-6) M increased the percentages of cells in S phase, and correspondingly decreased the percentages of cells in the G0/G1 phase. Tritium 38-40 prohibitin 2 Homo sapiens 95-98 7682962-5 1993 RP 67580, a selective NK1-receptor antagonist was found to displace the specific binding of [3H]substance P. Tritium 93-95 tachykinin receptor 1 Rattus norvegicus 22-34 2194839-3 1990 Northern blotting demonstrated the presence of HM1 transcripts in transformants, and crude membranes prepared from these cells showed saturable binding of the muscarinic antagonist [3H]N-methyl scopolamine with a Kd of 179 pM and Bmax of 20 fmol/mg protein. Tritium 182-184 cholinergic receptor muscarinic 1 Homo sapiens 47-50 7682962-6 1993 When [3H]RP 67580 was used as a ligand, it displayed a high affinity (KD value: 1.22 +/- 0.27 nM) in transfected cell homogenates and only competed with NK1 receptor ligands. Tritium 6-8 tachykinin receptor 1 Rattus norvegicus 153-165 7679727-8 1993 A good correlation was found when the abilities of NK1 receptor agonists for stimulating inositol phosphate production and for inhibiting [3H]substance P binding were compared. Tritium 139-141 tachykinin receptor 1 Homo sapiens 51-63 2160959-3 1990 Ca2+/diacylglycerol/phospholipid-dependent protein kinase C appeared to be the receptor for this binding because: a diacylglycerol, dioctanoylglycerol, competed with [3H]PDB for PMN binding sites; a blocker of protein kinase C-phospholipid interactions, sphinganine, inhibited PMN binding of [3H]PDB; and changes in cytosolic Ca2+ apparently regulated PMN binding of the label. Tritium 167-169 carbonic anhydrase 2 Homo sapiens 0-3 2160959-3 1990 Ca2+/diacylglycerol/phospholipid-dependent protein kinase C appeared to be the receptor for this binding because: a diacylglycerol, dioctanoylglycerol, competed with [3H]PDB for PMN binding sites; a blocker of protein kinase C-phospholipid interactions, sphinganine, inhibited PMN binding of [3H]PDB; and changes in cytosolic Ca2+ apparently regulated PMN binding of the label. Tritium 167-169 carbonic anhydrase 2 Homo sapiens 326-329 2160959-3 1990 Ca2+/diacylglycerol/phospholipid-dependent protein kinase C appeared to be the receptor for this binding because: a diacylglycerol, dioctanoylglycerol, competed with [3H]PDB for PMN binding sites; a blocker of protein kinase C-phospholipid interactions, sphinganine, inhibited PMN binding of [3H]PDB; and changes in cytosolic Ca2+ apparently regulated PMN binding of the label. Tritium 293-295 carbonic anhydrase 2 Homo sapiens 0-3 2160959-7 1990 A second ionophore, A23187, likewise induced Ca2(+)-dependent rises in [3H]PDB binding. Tritium 72-74 carbonic anhydrase 2 Homo sapiens 45-48 2160959-8 1990 These results fit the standard model, wherein rises in cytosolic Ca2+ cause protein kinase C to translocate from cytosol to plasmalemma and thereby become more available to [3H]PDB. Tritium 174-176 carbonic anhydrase 2 Homo sapiens 65-68 8436962-5 1993 GCP phospholipase A2 (PLA2) activity was demonstrated using [3H]-or [14C]AA-phosphatidylcholine (PC) or -PI as the substrate in vitro and GCPs or a cytosolic GCP extract as the source of enzyme. Tritium 61-63 phospholipase A2 group IB Rattus norvegicus 4-20 2167748-4 1990 Using radioligand binding techniques, we have demonstrated that 6 out of 6 glioblastoma (GBM) specimens had high concentrations of PBR [( 3H]PK 11195 binding sites) which were significantly greater than in 5 normal human frontal cortex samples. Tritium 138-140 translocator protein Homo sapiens 131-134 2167748-8 1990 The ligand recognition site of the PBR in human GBM tissue was photoaffinity labeled using [3H]PK 14105, a nitrophenyl analogue of PK 11195. Tritium 92-94 translocator protein Homo sapiens 35-38 2161774-3 1990 Within the hippocampal formation, loss of [3H]KA and NMDA-sensitive L-[3H]glutamate binding was apparent in the sclerotic regions CA3, hilus, and CA1. Tritium 43-45 carbonic anhydrase 1 Homo sapiens 146-149 2163016-1 1990 The ability of alpha 1a- and alpha 1b-adrenergic receptor subtypes to stimulate [3H]inositol phosphate [( 3H]InsP) formation was examined in collagenase-dispersed hepatocytes and renal cells. Tritium 81-83 adrenoceptor alpha 1B Homo sapiens 15-57 2186026-9 1990 CSF-1 induced mitogenesis with peak [3H] thymidine incorporation occurring 24 h after addition of the mitogen. Tritium 37-39 colony stimulating factor 1 (macrophage) Mus musculus 0-5 2335274-4 1990 Oral administration of 3H-labeled retinol 3 h before surgery resulted in 350% more tritium in the serum retinol-RBP-TTR complex of rats with acute renal failure as compared to sham-operated rats; this increase represented the fraction of retinol in RBP-TTR contributed by hepatic retinol from newly absorbed 3H-labeled retinol. Tritium 23-25 transthyretin Rattus norvegicus 116-119 8436962-5 1993 GCP phospholipase A2 (PLA2) activity was demonstrated using [3H]-or [14C]AA-phosphatidylcholine (PC) or -PI as the substrate in vitro and GCPs or a cytosolic GCP extract as the source of enzyme. Tritium 61-63 phospholipase A2 group IB Rattus norvegicus 22-26 2335274-4 1990 Oral administration of 3H-labeled retinol 3 h before surgery resulted in 350% more tritium in the serum retinol-RBP-TTR complex of rats with acute renal failure as compared to sham-operated rats; this increase represented the fraction of retinol in RBP-TTR contributed by hepatic retinol from newly absorbed 3H-labeled retinol. Tritium 23-25 transthyretin Rattus norvegicus 253-256 2335274-4 1990 Oral administration of 3H-labeled retinol 3 h before surgery resulted in 350% more tritium in the serum retinol-RBP-TTR complex of rats with acute renal failure as compared to sham-operated rats; this increase represented the fraction of retinol in RBP-TTR contributed by hepatic retinol from newly absorbed 3H-labeled retinol. Tritium 83-90 transthyretin Rattus norvegicus 116-119 8095334-6 1993 In situ hybridization of a 3H-labeled probe to human metaphase spreads localized the NDF gene to the short arm of chromosome 8 at bands p12-p21. Tritium 27-29 H3 histone pseudogene 16 Homo sapiens 140-143 2318143-2 1990 Continuous treatment with PTHrp for 24-72 h stimulated DNA synthesis, but inhibited [3H] proline incorporation into collagen by about 50%. Tritium 85-87 parathyroid hormone-like hormone Rattus norvegicus 26-31 2318143-3 1990 In contrast, transient exposure to PTHrp at 0.1-1.0 nM for 24 h followed by removal of the factor for 48 h caused an increase in [3H]proline incorporation into collagen and noncollagen protein by 2- and 1.6-fold, respectively. Tritium 130-132 parathyroid hormone-like hormone Rattus norvegicus 35-40 7679009-11 1993 In contrast, cells growing in Epo (32D Epo or 32D Epo1.1) spend relatively less time in G0/G1 and correspondingly more time in S. These data were confirmed by the analysis of the tritiated thymidine (3H-TdR) suicide rate and of the expression of cell cycle-specific genes. Tritium 200-202 erythropoietin Mus musculus 30-33 7679009-11 1993 In contrast, cells growing in Epo (32D Epo or 32D Epo1.1) spend relatively less time in G0/G1 and correspondingly more time in S. These data were confirmed by the analysis of the tritiated thymidine (3H-TdR) suicide rate and of the expression of cell cycle-specific genes. Tritium 200-202 erythropoietin Mus musculus 50-56 1971160-1 1990 A modification of the original microdistillation assay for lysyl oxidase is described in which Amicon C-10 microconcentrators are used to separate, by ultrafiltration, the 3H-labeled products released from a [4,5-3H]-lysine-labeled elastin substrate. Tritium 172-174 lysyl oxidase Homo sapiens 59-72 1971160-1 1990 A modification of the original microdistillation assay for lysyl oxidase is described in which Amicon C-10 microconcentrators are used to separate, by ultrafiltration, the 3H-labeled products released from a [4,5-3H]-lysine-labeled elastin substrate. Tritium 172-174 elastin Homo sapiens 232-239 8447422-7 1993 4) Immunoabsorption by a TGF-alpha-specific antisera reduced [3H]thymidine incorporation. Tritium 62-64 protransforming growth factor alpha Canis lupus familiaris 25-34 8419650-8 1993 Charge analysis of the [3H]galactose- and [3H]glucosamine-labeled glycopeptides from gp120 and gp160 indicates that approximately 14% of the complex-type N-linked oligosaccharides are sulfated. Tritium 24-26 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 85-90 8419650-8 1993 Charge analysis of the [3H]galactose- and [3H]glucosamine-labeled glycopeptides from gp120 and gp160 indicates that approximately 14% of the complex-type N-linked oligosaccharides are sulfated. Tritium 43-45 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 85-90 8381745-1 1993 This study investigated the role of cyclic AMP in the inhibition of [3H]5-hydroxytryptamine ([3H]5-HT) release mediated through the 5-HT1D autoreceptor using slices of guinea-pig cerebral cortex. Tritium 69-71 5-hydroxytryptamine receptor 1D Cavia porcellus 132-138 8419142-5 1993 In GH3 cells, both IL-2 (1-100 U/ml) and IL-6 (10-500 U/ml) significantly stimulated [3H]thymidine incorporation and cell count. Tritium 86-88 interleukin 2 Rattus norvegicus 19-23 8417130-8 1993 Killer cells also showed an enhanced capacity to proliferate in response to rIL-2 in a 6-day [3H]TdR incorporation assay. Tritium 94-96 interleukin 2 Rattus norvegicus 76-81 1460023-4 1992 Substitution of the putative fatty acylation sites, cysteines 3 and 4, abolished membrane association as well as [3H]palmitic acid labeling of neuromodulin. Tritium 114-116 neuromodulin Cricetulus griseus 143-155 1281744-5 1992 The order of potency of neurokinins to inhibit [3H][Sar9,Met(O2)11]SP binding from rat brain (SP > NKA > NKB) was found different of that observed on guinea pig brain (SP > NKB > NKA). Tritium 48-50 Natural killer alloreactivity QTL 1 Rattus norvegicus 102-105 1419027-5 1992 PAF was quantified by thin-layer chromatographic separation of lipid extracts from cells radiolabeled with [3H]lysoPAF and by radioimmunoassay. Tritium 108-110 PCNA clamp associated factor Homo sapiens 0-3 1419027-8 1992 Primary human bronchial epithelial cells also demonstrated significant increases in [3H]PAF release (average increase of 289% after exposure to 1.0 ppm O3 for 60 min) compared with paired air controls. Tritium 85-87 PCNA clamp associated factor Homo sapiens 88-91 1329656-4 1992 Moreover, using 10(-9) M [3H]TCDD, it was shown that the alpha NF- and MCDF-mediated antagonism of TCDD-induced CYP1A1 gene expression was paralleled by a decrease in levels of the nuclear [3H]TCDD-Ah receptor complex as determined by velocity sedimentation analysis of the nuclear extracts. Tritium 26-28 cytochrome P450 family 1 subfamily A member 1 Homo sapiens 112-118 1329656-4 1992 Moreover, using 10(-9) M [3H]TCDD, it was shown that the alpha NF- and MCDF-mediated antagonism of TCDD-induced CYP1A1 gene expression was paralleled by a decrease in levels of the nuclear [3H]TCDD-Ah receptor complex as determined by velocity sedimentation analysis of the nuclear extracts. Tritium 190-192 cytochrome P450 family 1 subfamily A member 1 Homo sapiens 112-118 1416995-4 1992 Modified enzyme, prepared by incubating glutathione S-transferase with [3H]S-BDB-G in the absence or in the presence of S-hexylglutathione, was reduced with NaBH4, reacted with N-ethylmaleimide, and digested with trypsin. Tritium 72-74 hematopoietic prostaglandin D synthase Rattus norvegicus 40-65 1480169-5 1992 Conditioned medium from MD-IGF-I cells, induced with DEX, stimulated [3H]thymidine incorporation into DNA of MAC-T cells and uninduced MD-IGF-I cells. Tritium 70-72 insulin like growth factor 1 Bos taurus 27-32 11537498-4 1992 Application of CaM antagonists at the root tip (> or = 1 micromole) also decreased the relative downward movement of label from 3H-IAA applied to the upper side of the elongation zone of gravistimulated roots. Tritium 131-133 calmodulin1 Zea mays 15-18 1284575-1 1992 The effects of the alpha 2-adrenoceptor agonist clonidine and antagonist yohimbine were investigated on 3,4-diaminopyridine(DAP)-evoked [3H]norepinephrine ([3H]NE) release from rat hippocampal slices in the presence and absence of extracellular Ca2+. Tritium 137-139 death-associated protein Rattus norvegicus 124-127 1284575-3 1992 [3H]overflow was evoked by addition of DAP 100 mumol.L-1 for 10 min to the superfusion medium. Tritium 1-3 death-associated protein Rattus norvegicus 39-42 1284575-4 1992 Clonidine and yohimbine inhibited and enhanced the 3,4-DAP-evoked [3H]NE release in a concentration-dependent manner both in the presence and absence of extracellular Ca2+. Tritium 67-69 death-associated protein Rattus norvegicus 55-58 1280526-2 1992 Sendide potently displaced [3H]-labeled substance P (SP) binding to mouse spinal cord membranes in a competitive manner. Tritium 28-30 tachykinin 1 Mus musculus 40-51 1280526-2 1992 Sendide potently displaced [3H]-labeled substance P (SP) binding to mouse spinal cord membranes in a competitive manner. Tritium 28-30 tachykinin 1 Mus musculus 53-55 1426017-1 1992 The affinities of a range of antipsychotic drugs at human hippocampal 5-HT1A receptors, defined by specific [3H]8-OH-DPAT binding, were determined. Tritium 109-111 5-hydroxytryptamine receptor 1A Homo sapiens 70-76 12106415-6 1992 The calcium-induced increase in [3H]AMPA binding is different among various brain structures, being larger in area CA1 of the hippocampus and in the superficial layers of the cerebral cortex. Tritium 33-35 carbonic anhydrase 1 Rattus norvegicus 115-118 1331748-9 1992 Using [3H]5-HT as the basis for a radioligand binding assay, the relative affinities of several tryptamine and piperazine derivatives for the cloned 5-HT2F receptor correlated with their relative potencies to contract the rat stomach fundus. Tritium 7-9 5-hydroxytryptamine receptor 2B Rattus norvegicus 149-155 1409580-6 1992 Furthermore, [3H]thymidine birth-dating experiments revealed that the majority of presumptive PC3.1-immunopositive neurons underwent their final mitosis around embryonic day 15, suggesting that the regional specification events for these neurons occur before their neurogenesis. Tritium 14-16 proprotein convertase subtilisin/kexin type 1 Rattus norvegicus 94-97 1530586-5 1992 However, neutrophils pretreated with GM-CSF showed a dramatic 25-40% fall in the free [3H]Ins content of the cell, which was almost quantitatively recovered in a 2-4-fold increase in radioactivity within PtdIns. Tritium 87-89 colony stimulating factor 2 Homo sapiens 37-43 1530586-7 1992 Interestingly, in comparison with controls, the GM-CSF-stimulated increases in [3H]polyphosphoinositide (including 3-phosphorylated lipids) and [3H]Ins polyphosphate contents were consistently higher than that observed with PtdIns. Tritium 80-82 colony stimulating factor 2 Homo sapiens 48-54 1530586-7 1992 Interestingly, in comparison with controls, the GM-CSF-stimulated increases in [3H]polyphosphoinositide (including 3-phosphorylated lipids) and [3H]Ins polyphosphate contents were consistently higher than that observed with PtdIns. Tritium 145-147 colony stimulating factor 2 Homo sapiens 48-54 1380434-6 1992 In particular, addition of increasing concentrations of EGF from 0.25-10 ng/ml to a constant concentration of 50 ng/ml IGF-I resulted in significant potentiation of [3H]thymidine incorporation of astroglial cells. Tritium 166-168 insulin-like growth factor 1 Rattus norvegicus 119-124 1505921-6 1992 In culture-activated lipocytes, interferon-gamma (1,000 U/ml) significantly inhibited lipocyte proliferation as assessed by [3H]thymidine incorporation assay and nuclear autoradiography. Tritium 125-127 interferon gamma Rattus norvegicus 32-57 1336805-8 1992 [14C]AA and [3H]cholesteryl ester uptake were influenced to similar extents by factors unknown to regulate the LDL receptor and by an anti-LDL receptor antibody. Tritium 13-15 low density lipoprotein receptor Homo sapiens 111-123 1336805-10 1992 Using an anti-LDL receptor antibody, 25.0% of [3H]cholesterol/cholesteryl ester and 37.7% of [14C]AA binding to the cells at 4 degrees C were blocked. Tritium 47-49 low density lipoprotein receptor Homo sapiens 14-26 1354232-4 1992 i) Hydrolysis of alkyl-acyl-GPC upon CD2 stimulation was evidenced: [3H]alkyl-lyso-GPC was formed from [3H]alkyl-acyl-GPC in [3H] alkyl-labeled cells; alkyl-lyso-GPC production was also bioassayed after CD2 triggering. Tritium 104-106 CD2 molecule Homo sapiens 203-206 1354232-4 1992 i) Hydrolysis of alkyl-acyl-GPC upon CD2 stimulation was evidenced: [3H]alkyl-lyso-GPC was formed from [3H]alkyl-acyl-GPC in [3H] alkyl-labeled cells; alkyl-lyso-GPC production was also bioassayed after CD2 triggering. Tritium 104-106 CD2 molecule Homo sapiens 37-40 1354232-4 1992 i) Hydrolysis of alkyl-acyl-GPC upon CD2 stimulation was evidenced: [3H]alkyl-lyso-GPC was formed from [3H]alkyl-acyl-GPC in [3H] alkyl-labeled cells; alkyl-lyso-GPC production was also bioassayed after CD2 triggering. Tritium 104-106 CD2 molecule Homo sapiens 203-206 1354232-5 1992 ii) The rate of arachidonate transfer from diacyl-GPC to alkyl-acyl-GPC increased after CD2 stimulation of the [3H]arachidonate-labeled P28D T cells, demonstrating alkyl-lyso-GPC formation. Tritium 112-114 CD2 molecule Homo sapiens 88-91 1322030-11 1992 In displacement experiments PAF (0.01 to 10000 nmol/L) and WEB 2086 (0.01 to 10000 nmol/L), but not lyso-PAF, completely displaced 3H-WEB 2086 from its binding sites on human and lung membranes. Tritium 131-133 PCNA clamp associated factor Homo sapiens 28-31 1458972-4 1992 The apparent recovery of liver function and the increase of 3H-TdR incorporation into hepatic DNA correlated with the morphologic changes observed under light and electron microscopes, showing that the damages inflicted on the cellular and subcellular structure in the liver of hHSS-treated rats were greatly alleviated and rapidly repaired. Tritium 60-62 growth factor, augmenter of liver regeneration Homo sapiens 278-282 1321849-7 1992 The activity of acetyl CoA:2-lyso-PAF acetyltransferase was transiently increased in porin-stimulated PMN and the [3H]acetyl group was incorporated in the synthetized PAF after cell preincubation with [3H]acetyl CoA. Tritium 115-117 PCNA clamp associated factor Homo sapiens 34-37 1321849-7 1992 The activity of acetyl CoA:2-lyso-PAF acetyltransferase was transiently increased in porin-stimulated PMN and the [3H]acetyl group was incorporated in the synthetized PAF after cell preincubation with [3H]acetyl CoA. Tritium 202-204 PCNA clamp associated factor Homo sapiens 34-37 1321856-12 1992 Scatchard plot analysis of radioligand binding experiments indicated that 3G8 Fab did not significantly alter the number of FMLP binding sites on neutrophils but significantly increased the affinity of the FPR for [3H]FMLP. Tritium 215-217 FA complementation group B Homo sapiens 78-81 1385724-13 1992 Preparations of 6S and 10S [3H]myosin were digested exhaustively with trypsin. Tritium 28-30 myosin heavy chain 14 Homo sapiens 31-37 1514698-3 1992 Examination of the binding profile of tritium-labeled PAF to these platelets in the presence or absence of ADP revealed significant difference in the Kd values but not in the number of specific binding sites. Tritium 38-45 PCNA clamp associated factor Homo sapiens 54-57 1512072-2 1992 The stimulation of PHA blasts with rIL-2 resulted in an enhancement of tritiated thymidine ([3H]TdR) incorporation and of soluble interleukin-2 receptor (sIL-2R) release. Tritium 93-95 interleukin 2 Rattus norvegicus 35-40 1512072-3 1992 Cyclosporin A (CsA) and prednisolone inhibited in different ways the responsiveness of PHA pre-stimulated blood mononuclear cells (PBMC) to rIL-2, as measured by [3H]TdR incorporation. Tritium 163-165 interleukin 2 Rattus norvegicus 140-145 1512072-9 1992 Stimulation of these blasts with rIL-2 resulted in higher [3H]TdR incorporation by CD8+ blasts than by CD4+ blasts: however, no sIL-2R release was detected in supernatants of either CD4+ or of CD8+ blasts. Tritium 59-61 interleukin 2 Rattus norvegicus 33-38 1512072-10 1992 Both CsA and prednisolone inhibited the rIL-2-induced enhancement of [3H]TdR incorporation by both T-cell subsets. Tritium 70-72 interleukin 2 Rattus norvegicus 40-45 1378096-4 1992 In binding experiments, FK 224 dose-dependently inhibited [3H]SP binding to rat cerebral cortical membranes (NK1) and [3H]neurokinin (NK) A (NKA) binding to rat duodenum smooth muscle membranes (NK2), but did not affect [3H]eledoisin binding to rat cerebral cortical membranes (NK3). Tritium 119-121 Natural killer alloreactivity QTL 1 Rattus norvegicus 141-144 1378096-4 1992 In binding experiments, FK 224 dose-dependently inhibited [3H]SP binding to rat cerebral cortical membranes (NK1) and [3H]neurokinin (NK) A (NKA) binding to rat duodenum smooth muscle membranes (NK2), but did not affect [3H]eledoisin binding to rat cerebral cortical membranes (NK3). Tritium 119-121 Natural killer alloreactivity QTL 1 Rattus norvegicus 141-144 1378162-5 1992 However, SCF potentiated the stimulatory effect of GM-CSF, G-CSF, and IL-3 on both 3H-TdR incorporation and colony formation. Tritium 83-85 colony stimulating factor 2 Homo sapiens 51-57 1378162-5 1992 However, SCF potentiated the stimulatory effect of GM-CSF, G-CSF, and IL-3 on both 3H-TdR incorporation and colony formation. Tritium 83-85 colony stimulating factor 3 Homo sapiens 59-64 1378162-5 1992 However, SCF potentiated the stimulatory effect of GM-CSF, G-CSF, and IL-3 on both 3H-TdR incorporation and colony formation. Tritium 83-85 interleukin 3 Homo sapiens 70-74 1322575-6 1992 In cells cotreated with TCDD plus 100 ng/ml insulin or 10 nM 17 beta-estradiol, EGF, TGF-alpha and IGF-I, TCDD caused a concentration-dependent decrease in cell proliferation and [3H]thymidine uptake. Tritium 180-182 epidermal growth factor Homo sapiens 80-83 1375845-6 1992 Proliferation of AA cells cultured in a liquid medium containing SCF together with Epo, IL-3, GM-CSF, and G-CSF approached 70% of the control level, as measured by 3H-thymidine incorporation. Tritium 164-166 colony stimulating factor 3 Homo sapiens 106-111 1425452-7 1992 When IL-1 and TNF alpha were added together in relatively low concentrations (1 ng/ml each), the combination had synergistic effects in enhancing [3H]thymidine incorporation. Tritium 147-149 interleukin 1 alpha Homo sapiens 5-9 1387900-3 1992 Trophoblast cells are also observed to show increased DNA synthesis in response to GM-CSF as assayed by [3H]thymidine incorporation and proliferative activity as demonstrated by double immunocytochemical staining of cells with a trophoblast cytokeratin marker (PKK1) and for the nuclear proliferation antigen (Ki-67). Tritium 105-107 colony stimulating factor 2 Homo sapiens 83-89 1613425-0 1992 Eel insulin: isolation, characterization and stimulatory actions on [35S]sulphate and [3H]thymidine uptake in the branchial cartilage of the eel in vitro. Tritium 87-89 insulin Bos taurus 4-11 1560018-3 1992 Under optimal conditions, the rate of deacylation was linear up to 20 min, and the apparent Km for bovine [3H]palmitoyl-PLP was 18 microM. Tritium 107-109 proteolipid protein 1 Bos taurus 120-123 1532344-3 1992 This acyclonucleoside analogue is an inhibitor of thymidylate synthase (TS) as determined by inhibition of [6-3H]-2"-deoxyuridine incorporation into DNA, inhibition of 3H release from [5-3H]-2"-deoxyuridine, and decrease in both the free and total TS 5"-fluoro-2"-deoxyuridine 5"-monophosphate binding sites. Tritium 110-112 thymidylate synthetase Homo sapiens 50-70 1555893-3 1992 Basal medium conditioned by ZL5 cells possesses a mitogenic activity with an autocrine effect, as measured by cell counting and by a 3H-thymidine incorporation assay. Tritium 133-135 small nucleolar RNA, C/D box 143 Homo sapiens 28-31 1387436-5 1992 Scatchard analysis of equilibrium binding using the thromboxane antagonist, [3H]-SQ29548, demonstrated one class of high affinity thromboxane receptor sites in control (Kd = 19.9 +/- 2.6 nM, N = 16) and diabetic rats (Kd = 19.8 +/- 2.1 nM, N = 8, P = NS). Tritium 77-79 thromboxane A2 receptor Rattus norvegicus 130-150 1541904-3 1992 After incubation for 1 h at 37 degrees C, formation of [3H]acyl-PAF from either [3H]lyso-PAF or [3H]PAF increased significantly in PMNs from infected guinea pigs compared to control PMNs. Tritium 56-59 PCNA clamp associated factor Homo sapiens 64-67 1541904-3 1992 After incubation for 1 h at 37 degrees C, formation of [3H]acyl-PAF from either [3H]lyso-PAF or [3H]PAF increased significantly in PMNs from infected guinea pigs compared to control PMNs. Tritium 56-59 PCNA clamp associated factor Homo sapiens 89-92 1541904-3 1992 After incubation for 1 h at 37 degrees C, formation of [3H]acyl-PAF from either [3H]lyso-PAF or [3H]PAF increased significantly in PMNs from infected guinea pigs compared to control PMNs. Tritium 56-59 PCNA clamp associated factor Homo sapiens 89-92 1542723-5 1992 Spleen cells from the FTS-treated mice incorporated more [3H]thymidine in culture with or without concanavalin A. Tritium 58-60 AKT interacting protein Mus musculus 22-25 1540182-2 1992 The [3H] proline incorporation into the collagenase digestible protein(CDP) and the percent collagen synthesis were significantly decreased in the c-fos transfectants which constitutively express c-fos mRNA as compared with control transfectants. Tritium 5-7 cut-like homeobox 1 Mus musculus 71-74 1540182-2 1992 The [3H] proline incorporation into the collagenase digestible protein(CDP) and the percent collagen synthesis were significantly decreased in the c-fos transfectants which constitutively express c-fos mRNA as compared with control transfectants. Tritium 5-7 FBJ osteosarcoma oncogene Mus musculus 147-152 1540182-2 1992 The [3H] proline incorporation into the collagenase digestible protein(CDP) and the percent collagen synthesis were significantly decreased in the c-fos transfectants which constitutively express c-fos mRNA as compared with control transfectants. Tritium 5-7 FBJ osteosarcoma oncogene Mus musculus 196-201 1304863-3 1992 The so-called muscarinic toxins (MTX1 and MTX2) have been shown to inhibit the specific binding of [3H]QNB (0.15 nM), [3H]PZ (2.5 nM) and [3H]oxoM (2 nM) to bovine cerebral cortex membranes by 60, 88 and 82% respectively. Tritium 100-102 metaxin 1 Bos taurus 33-37 1304863-3 1992 The so-called muscarinic toxins (MTX1 and MTX2) have been shown to inhibit the specific binding of [3H]QNB (0.15 nM), [3H]PZ (2.5 nM) and [3H]oxoM (2 nM) to bovine cerebral cortex membranes by 60, 88 and 82% respectively. Tritium 119-121 metaxin 1 Bos taurus 33-37 1304863-3 1992 The so-called muscarinic toxins (MTX1 and MTX2) have been shown to inhibit the specific binding of [3H]QNB (0.15 nM), [3H]PZ (2.5 nM) and [3H]oxoM (2 nM) to bovine cerebral cortex membranes by 60, 88 and 82% respectively. Tritium 119-121 metaxin 1 Bos taurus 33-37 1623167-3 1992 Application of exogenous IL-2 to striatal slices in in vitro produced significant increases in both spontaneous and potassium (25 mM)-evoked [3H] dopamine release. Tritium 142-144 interleukin 2 Rattus norvegicus 25-29 1350989-6 1992 A similar specific striatal binding of [3H]GBR 12783 was evidenced in both NMRI and CD1 strains. Tritium 40-42 CD1 antigen complex Mus musculus 84-87 1636501-2 1992 Preincubation of cortical synaptosomal membranes of rat brain with phospholipase A2 (PLA2) increases the number of [3H]imipramine ([3H]IMI) high affinity binding sites without altering Kd (Bmax control: 2.53 +/- 0.28 pmol/mg protein vs Bmax PLA2: 3.66 +/- 0.26 pmol/mg protein). Tritium 116-118 phospholipase A2 group IB Rattus norvegicus 67-83 1636501-2 1992 Preincubation of cortical synaptosomal membranes of rat brain with phospholipase A2 (PLA2) increases the number of [3H]imipramine ([3H]IMI) high affinity binding sites without altering Kd (Bmax control: 2.53 +/- 0.28 pmol/mg protein vs Bmax PLA2: 3.66 +/- 0.26 pmol/mg protein). Tritium 116-118 phospholipase A2 group IB Rattus norvegicus 85-89 1636501-2 1992 Preincubation of cortical synaptosomal membranes of rat brain with phospholipase A2 (PLA2) increases the number of [3H]imipramine ([3H]IMI) high affinity binding sites without altering Kd (Bmax control: 2.53 +/- 0.28 pmol/mg protein vs Bmax PLA2: 3.66 +/- 0.26 pmol/mg protein). Tritium 132-134 phospholipase A2 group IB Rattus norvegicus 67-83 1636501-2 1992 Preincubation of cortical synaptosomal membranes of rat brain with phospholipase A2 (PLA2) increases the number of [3H]imipramine ([3H]IMI) high affinity binding sites without altering Kd (Bmax control: 2.53 +/- 0.28 pmol/mg protein vs Bmax PLA2: 3.66 +/- 0.26 pmol/mg protein). Tritium 132-134 phospholipase A2 group IB Rattus norvegicus 85-89 1370604-2 1992 Both gp120 and gp160, at concentrations from 0.01 to 10 micrograms/ml, caused a progressive and significant (p less than 0.05) decrease in viable CD34+ cell count in liquid cultures supplemented with 2 ng/ml of human recombinant (r) interleukin-3 (IL-3), evaluated by means of Trypan-blue exclusion and [3H]thymidine ([3H]TdR) incorporation. Tritium 304-306 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 5-10 1370604-2 1992 Both gp120 and gp160, at concentrations from 0.01 to 10 micrograms/ml, caused a progressive and significant (p less than 0.05) decrease in viable CD34+ cell count in liquid cultures supplemented with 2 ng/ml of human recombinant (r) interleukin-3 (IL-3), evaluated by means of Trypan-blue exclusion and [3H]thymidine ([3H]TdR) incorporation. Tritium 319-321 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 5-10 1728582-6 1992 Addition of exogenous EGF to lungs in culture resulted in significant concentration-dependent stimulation of branching morphogenesis, DNA, RNA, and protein content, and in [3H]thymidine incorporation into DNA. Tritium 173-175 epidermal growth factor Mus musculus 22-25 1728582-7 1992 Conversely, the addition of tyrphostin (specific EGF receptor kinase antagonist) to lungs in culture resulted in concentration-dependent inhibition of branching morphogenesis, DNA, RNA, and protein content, and in [3H]thymidine incorporation into DNA without apparent cytotoxicity. Tritium 215-217 epidermal growth factor Mus musculus 49-52 1389476-2 1992 Inhibition of DNA synthesis, as measured by [3H] thymidine incorporation, occurred after 6 h of incubation with rIFN-gamma or rTNF-alpha, and this action was potentiated when the two cytokines were applied simultaneously. Tritium 45-47 interferon gamma Rattus norvegicus 112-122 1419069-3 1992 (3H) thymidine incorporation assays with intermolecular cystine-bonded or monomeric mIL2 revealed almost identical median effective concentrations (EC50) and high-affinity dissociation constants (Kdh), respectively. Tritium 1-3 interleukin 2 Mus musculus 84-88 1603334-5 1992 These results suggest that thymopoietin selectively interacts with the nicotinic alpha-bungarotoxin binding site labelled by [125I]alpha-bungarotoxin rather than the neuronal nicotinic receptor(s) labelled by [3H]nicotine. Tritium 210-212 thymopoietin Rattus norvegicus 27-39 1665204-5 1991 In addition, while insulin-stimulated oocyte PDE activity measured in vivo after microinjection of 200 microM [3H] cAMP was inhibited by nonselective and type III-specific drugs (with IC50 values of 4.2 +/- 1.8 microM Cl-930 and 26 +/- 6 microM imazodan), type IV and type V inhibitors did not inhibit hormone-stimulated enzyme activity. Tritium 111-113 insulin S homeolog Xenopus laevis 19-26 1717302-5 1991 In combination with rhuGM-CSF (250 U/ml) or IL-3 (500 U/ml), rmuMGF (25 ng/ml) enhanced [3H]thymidine uptake two- to sevenfold greater than the sum of the effects of each factor alone. Tritium 89-91 interleukin 3 Homo sapiens 44-48 1935798-3 1991 IL-1 alpha stimulated [3H]thymidine incorporation. Tritium 23-25 interleukin 1 alpha Mus musculus 0-10 1935798-5 1991 Both cortisol and IL-1 alpha decreased the incorporation of [3H]proline into collagenase-digestible protein (CDP) and reduced alpha 1(I)procollagen mRNA levels at 72 h. Noncollagen protein (NCP) labeling was increased by IL-1 and decreased by cortisol. Tritium 61-63 interleukin 1 alpha Mus musculus 18-28 1935798-5 1991 Both cortisol and IL-1 alpha decreased the incorporation of [3H]proline into collagenase-digestible protein (CDP) and reduced alpha 1(I)procollagen mRNA levels at 72 h. Noncollagen protein (NCP) labeling was increased by IL-1 and decreased by cortisol. Tritium 61-63 cut-like homeobox 1 Mus musculus 77-107 1655876-2 1991 Autoradiographic localization of [3H]-trandolaprilate in rabbit fundus sections shows that ACE is present in the fundic mucosa and mainly in the gland area. Tritium 34-36 angiotensin-converting enzyme Oryctolagus cuniculus 91-94 1686902-4 1991 The [3H]PDBu binding activities in the strata oriens of the CA1 and CA3 subfields and the molecular layer of the dentate gyrus in the rat hippocampus were significantly higher than in the gerbil hippocampus. Tritium 5-7 carbonic anhydrase 1 Rattus norvegicus 60-63 1686910-2 1991 4-Aminopyridine (4-AP; 10 and 30 microM) and 3,4-diaminopyridine (3,4-DAP; 30 microM) markedly increased the basal tritium outflow, whereas tetraethylammonium (TEA; 100-1000 microM) was without effect. Tritium 115-122 death-associated protein Rattus norvegicus 70-73 1776129-5 1991 The autoradiographic distribution of [3H]4-DAMP sites revealed a discrete pattern of labeling in the rat brain, with the highest densities of [3H]4-DAMP sites present in the CA1 sub-field of Ammon"s horn of the hippocampus, the dentate gyrus, the olfactory tubercle, the external plexiform layer of the olfactory bulb and layers I-II of the frontoparietal cortex. Tritium 38-40 carbonic anhydrase 1 Rattus norvegicus 174-177 1776129-5 1991 The autoradiographic distribution of [3H]4-DAMP sites revealed a discrete pattern of labeling in the rat brain, with the highest densities of [3H]4-DAMP sites present in the CA1 sub-field of Ammon"s horn of the hippocampus, the dentate gyrus, the olfactory tubercle, the external plexiform layer of the olfactory bulb and layers I-II of the frontoparietal cortex. Tritium 143-145 carbonic anhydrase 1 Rattus norvegicus 174-177 1931944-1 1991 The active-site topology of smooth muscle myosin has been investigated by direct photoaffinity-labeling studies with [3H]ADP. Tritium 118-120 myosin heavy chain 14 Homo sapiens 42-48 1892886-10 1991 Treatment of the cells with dimethylethanolamine increased the formation of [3H]GPC by 5-fold, while the production of lysoPC was inhibited by 60%. Tritium 77-79 glycophorin C Rattus norvegicus 80-83 1838900-1 1991 Endogenous phospholipase A2 (PLA2) specific activity (SA) (% hydrolysis/min/mg protein) in the rat pleural cavity, measured as the acyl-hydrolysis of [3H]-arachidonic acid E. coli substrate, was quantitated after induction of a reverse passive Arthus reaction (RPAR). Tritium 151-153 phospholipase A2 group IB Rattus norvegicus 11-27 1838900-1 1991 Endogenous phospholipase A2 (PLA2) specific activity (SA) (% hydrolysis/min/mg protein) in the rat pleural cavity, measured as the acyl-hydrolysis of [3H]-arachidonic acid E. coli substrate, was quantitated after induction of a reverse passive Arthus reaction (RPAR). Tritium 151-153 phospholipase A2 group IB Rattus norvegicus 29-33 1661214-0 1991 Opposite effects of phospholipase A2 on [3H]AMPA binding in adult and neonatal membranes. Tritium 41-43 phospholipase A2 group IB Rattus norvegicus 20-36 1661214-1 1991 In the present study, we compared the effect of phospholipase A2 (PLA2) treatment of synaptic membranes from adult and neonatal rats on the characteristics of [3H]AMPA binding sites. Tritium 160-162 phospholipase A2 group IB Rattus norvegicus 48-64 1661214-1 1991 In the present study, we compared the effect of phospholipase A2 (PLA2) treatment of synaptic membranes from adult and neonatal rats on the characteristics of [3H]AMPA binding sites. Tritium 160-162 phospholipase A2 group IB Rattus norvegicus 66-70 1661214-2 1991 Whereas PLA2 treatment of membranes from adult rats produces an increased affinity for [3H]AMPA binding, the same treatment in neonatal rats results in a decrease in the maximal number of binding sites. Tritium 88-90 phospholipase A2 group IB Rattus norvegicus 8-12 1661214-3 1991 Since activation of PLA2 has been proposed to play a critical role in the formation of long-term potentiation (LTP), possibly mediated through a modification of the AMPA receptors, the results strengthen the hypothesis that PLA2-induced modification of [3H]AMPA binding sites is an important component of synaptic plasticity. Tritium 254-256 phospholipase A2 group IB Rattus norvegicus 20-24 1661214-3 1991 Since activation of PLA2 has been proposed to play a critical role in the formation of long-term potentiation (LTP), possibly mediated through a modification of the AMPA receptors, the results strengthen the hypothesis that PLA2-induced modification of [3H]AMPA binding sites is an important component of synaptic plasticity. Tritium 254-256 phospholipase A2 group IB Rattus norvegicus 224-228 1907991-11 1991 By contrast, anti-Ig by itself stimulates significant increases in [3H]thymidine incorporation that is inhibited by PMA. Tritium 68-70 chemokine (C-X-C motif) ligand 9 Mus musculus 18-20 1713161-5 1991 Incubation of cells with IGF-I resulted in time- and dose-dependent decreases in [125I]IGF-I binding and IGF-I stimulated [3H]aminoisobutyric acid uptake and [3H]thymidine incorporation. Tritium 159-161 insulin like growth factor 1 Bos taurus 25-30 1713161-5 1991 Incubation of cells with IGF-I resulted in time- and dose-dependent decreases in [125I]IGF-I binding and IGF-I stimulated [3H]aminoisobutyric acid uptake and [3H]thymidine incorporation. Tritium 159-161 insulin like growth factor 1 Bos taurus 87-92 1713161-5 1991 Incubation of cells with IGF-I resulted in time- and dose-dependent decreases in [125I]IGF-I binding and IGF-I stimulated [3H]aminoisobutyric acid uptake and [3H]thymidine incorporation. Tritium 159-161 insulin like growth factor 1 Bos taurus 87-92 1713161-6 1991 Preincubation with 4 nM IGF-I resulted in a 50-60% decrease in IGF-I receptor binding, accompanied by marked decreases in IGF-I-stimulated [3H]aminoisobutyric acid uptake (50-60%) and [3H]thymidine incorporation (80-90%). Tritium 140-142 insulin like growth factor 1 Bos taurus 24-29 1713161-6 1991 Preincubation with 4 nM IGF-I resulted in a 50-60% decrease in IGF-I receptor binding, accompanied by marked decreases in IGF-I-stimulated [3H]aminoisobutyric acid uptake (50-60%) and [3H]thymidine incorporation (80-90%). Tritium 185-187 insulin like growth factor 1 Bos taurus 24-29 1744985-0 1991 Effect of phospholipase A2 on temperature-induced high-affinity [3H]tryptamine binding sites in rat brain. Tritium 65-67 phospholipase A2 group IB Rattus norvegicus 10-26 1744985-2 1991 When the phospholipase A2-treated membranes were exposed to 1% bovine serum albumin (BSA) before assaying for [3H]tryptamine binding, a complete dose-dependent inhibition curve was observed. Tritium 111-113 phospholipase A2 group IB Rattus norvegicus 9-25 1744985-3 1991 At a concentration of 0.03 U, the action of phospholipase A2 resulted in the splitting of phosphatidylserine (PS), choline phosphatides (PC) and ethanolamine phosphatides (PE) by about 32, 34 and 65%, respectively, and reduced [3H]ligand binding by about 32%. Tritium 228-230 phospholipase A2 group IB Rattus norvegicus 44-60 1744985-5 1991 Moreover, Scatchard analysis of the [3H]tryptamine binding showed that phospholipase A2 drastically increased only the KD value of the high affinity sites, and this was accompanied by a decrement of the Bmax values of both the high and low affinity binding sites. Tritium 37-39 phospholipase A2 group IB Rattus norvegicus 71-87 1714275-4 1991 Gastrin (HG-17), CCK-39 and CCK-8 induced [3H]-inositol phosphate ([3H]InsP) accumulation from [3H]inositol prelabelled cells with a high potency (EC50: 0.3-2.7 nM) but CCK-8 exhibited a higher efficacy than HG-17 or CCK-39. Tritium 43-45 cholecystokinin Oryctolagus cuniculus 17-20 1714275-4 1991 Gastrin (HG-17), CCK-39 and CCK-8 induced [3H]-inositol phosphate ([3H]InsP) accumulation from [3H]inositol prelabelled cells with a high potency (EC50: 0.3-2.7 nM) but CCK-8 exhibited a higher efficacy than HG-17 or CCK-39. Tritium 43-45 cholecystokinin Oryctolagus cuniculus 28-31 1714275-4 1991 Gastrin (HG-17), CCK-39 and CCK-8 induced [3H]-inositol phosphate ([3H]InsP) accumulation from [3H]inositol prelabelled cells with a high potency (EC50: 0.3-2.7 nM) but CCK-8 exhibited a higher efficacy than HG-17 or CCK-39. Tritium 43-45 cholecystokinin Oryctolagus cuniculus 28-31 1714275-4 1991 Gastrin (HG-17), CCK-39 and CCK-8 induced [3H]-inositol phosphate ([3H]InsP) accumulation from [3H]inositol prelabelled cells with a high potency (EC50: 0.3-2.7 nM) but CCK-8 exhibited a higher efficacy than HG-17 or CCK-39. Tritium 43-45 cholecystokinin Oryctolagus cuniculus 28-31 1864372-0 1991 The binding of [3H]R-PIA to A1 adenosine receptors produces a conversion of the high- to the low-affinity state. Tritium 16-18 RPTOR independent companion of MTOR complex 2 Homo sapiens 21-24 1657282-5 1991 Quantitative autoradiography of 3H-PK-11195 binding 1 or 2 weeks after colchicine injection indicated that the increase in binding was restricted to the dorsal hippocampus both rostrally and caudally and was present in the dentate gyrus and CA1. Tritium 32-34 carbonic anhydrase 1 Rattus norvegicus 241-244 1678605-2 1991 DPM increased, dose-dependently, the intracellular accumulation of [3H]vinblastine in PLC/COL. Tritium 67-71 heparan sulfate proteoglycan 2 Homo sapiens 86-89 1940639-7 1991 SPT activity also increased after barrier disruption, peaking at 6 h (150%) (P less than 0.05), and returning towards normal by 24 h. Artificial restoration of the barrier with a water vapor-impermeable membrane prevented the increases in both [3H]H2O incorporation into sphingolipids and enzyme activity. Tritium 245-247 alanine--glyoxylate and serine--pyruvate aminotransferase Homo sapiens 0-3 1645554-1 1991 Treatment of wild-type Hepa 1c1c7 cells with 1 nM [3H]-17 beta-estradiol resulted in the rapid accumulation of the nuclear estrogen receptor complex whose levels were maximized within 1 hr. Tritium 51-53 estrogen receptor 1 (alpha) Mus musculus 123-140 1655161-2 1991 Using [3H][Met5]-enkephalin, a potent ligand involved in neural growth, particularly cell proliferation, specific and saturable binding was detected in homogenates of 6-day-old rat cerebellum; the data were consistent with a single binding site. Tritium 7-10 proenkephalin Rattus norvegicus 17-27 1655161-8 1991 In the adult rat cerebellum, non-opioid receptor binding of [3H][Met5]-enkephalin was recorded, mu and kappa receptors were also found in the developing rat cerebellum, while mu, delta, and kappa receptors were recorded in adult cerebellar tissue. Tritium 61-63 proenkephalin Rattus norvegicus 71-81 1828072-14 1991 A single 19-residue [3H]BzADP-labeled peptide was resolved from a tryptic digest of MF1, and this peptide corresponded with the one believed to contain at least a portion of the beta subunit catalytic site domain (i.e. beta Ala-338----beta Arg-356). Tritium 21-23 flap structure-specific endonuclease 1 Homo sapiens 84-87 1680719-0 1991 [3H]rauwolscine behaves as an agonist for the 5-HT1A receptors in human frontal cortex membranes. Tritium 1-3 5-hydroxytryptamine receptor 1A Homo sapiens 46-52 1652780-3 1991 DBI mRNA was detected by in situ hybridization using a 35S-labelled single-stranded RNA probe complementary to DBI mRNA and PBR by in vitro autoradiography using [3H]PK11195 as the ligand. Tritium 163-165 diazepam binding inhibitor Rattus norvegicus 0-3 1914143-4 1991 Moreover, morphine treatment inhibited [3H]thymidine incorporation by glial fibrillary acidic protein (GFAP) immunoreactive, flat (type 1) astrocytes, suggesting that the decrease in glial cell production was due in part to an inhibition of astrocyte proliferation. Tritium 40-42 glial fibrillary acidic protein Mus musculus 70-101 1914143-4 1991 Moreover, morphine treatment inhibited [3H]thymidine incorporation by glial fibrillary acidic protein (GFAP) immunoreactive, flat (type 1) astrocytes, suggesting that the decrease in glial cell production was due in part to an inhibition of astrocyte proliferation. Tritium 40-42 glial fibrillary acidic protein Mus musculus 103-107 1713869-10 1991 The maximal effect of insulin-like growth factor-I on 3H-thymidine incorporation was 71 +/- 16% higher than the maximal effect of insulin. Tritium 54-56 insulin-like growth factor 1 Rattus norvegicus 22-50 1862226-4 1991 Synthesis of [3H] PAF was measured by counts comigrating on TLC with unlabeled PAF. Tritium 14-16 PCNA clamp associated factor Homo sapiens 18-21 1862226-4 1991 Synthesis of [3H] PAF was measured by counts comigrating on TLC with unlabeled PAF. Tritium 14-16 PCNA clamp associated factor Homo sapiens 79-82 1648988-2 1991 [3H]Tandospirone binding was notably high in the dentate gyrus and CA1 area of the hippocampus, lateral septum, entorhinal cortex, interpeduncular nucleus and dorsal raphe nucleus. Tritium 1-3 carbonic anhydrase 1 Rattus norvegicus 67-70 2007563-2 1991 PLD and PI-PLC activities in these four transfected cell lines as well as in nontransfected cells were measured by the formation of [3H]phosphatidylethanol [( 3H]PEt) and [3H]inositol phosphates [( 3H]IP) after labeling cellular phospholipids with [3H]oleic acid and [3H]inositol. Tritium 133-135 phospholipase C epsilon 1 Homo sapiens 8-14 2007563-2 1991 PLD and PI-PLC activities in these four transfected cell lines as well as in nontransfected cells were measured by the formation of [3H]phosphatidylethanol [( 3H]PEt) and [3H]inositol phosphates [( 3H]IP) after labeling cellular phospholipids with [3H]oleic acid and [3H]inositol. Tritium 159-161 phospholipase C epsilon 1 Homo sapiens 8-14 2007563-2 1991 PLD and PI-PLC activities in these four transfected cell lines as well as in nontransfected cells were measured by the formation of [3H]phosphatidylethanol [( 3H]PEt) and [3H]inositol phosphates [( 3H]IP) after labeling cellular phospholipids with [3H]oleic acid and [3H]inositol. Tritium 159-161 phospholipase C epsilon 1 Homo sapiens 8-14 2025578-6 1991 Elevated c-myc mRNA expressions were well related to the in vitro proliferation (3H-TdR uptake), but not to IL-6 response. Tritium 81-83 MYC proto-oncogene, bHLH transcription factor Homo sapiens 9-14 2011576-6 1991 A 20-residue synthetic peptide (termed 2.1) from a non-Ca2(+)-binding, disulfide-rich domain of SPARC also exhibited a dose-dependent inhibition of [3H]thymidine uptake in endothelial cells within 24 hr after release from G0 phase. Tritium 149-151 secreted protein acidic and cysteine rich Bos taurus 96-101 2011576-9 1991 Maximum inhibition of [3H]thymidine uptake by SPARC and peptide 2.1 occurred during the early-to-middle G1 phase of the endothelial-cell cycle. Tritium 23-25 secreted protein acidic and cysteine rich Bos taurus 46-51 1705831-12 1991 Finally, highly purified adult PB BFU-E were stimulated into cycling by a 3-day treatment with interleukin-3 in liquid phase: both the sensitivity to c-myb antisense oligomers and the 3H-TdR suicide index showed a gradual, strictly parallel increase. Tritium 184-186 interleukin 3 Homo sapiens 95-108 1705837-2 1991 TF activity was measured as the ability of monolayers or cell lysates to support FVIIa-catalyzed activation peptide release from 3H-FX. Tritium 129-131 coagulation factor III, tissue factor Homo sapiens 0-2 1706663-4 1991 Since almost all [3H]galactose incorporated into the AsAg-Tf was released by digestion with streptococcal beta-galactosidase, most of the linkage created by this enzyme was in the Gal beta 1-4GlcNAc group. Tritium 18-20 galactosidase, beta 1 Mus musculus 106-124 1647849-5 1991 D-Pen2,L-Pen5-enkephalin (0.1-10 microM), a selective delta-opioid receptor agonist, produced a dose-related inhibition of the 25 mM K(+)-evoked tritium release. Tritium 145-152 proenkephalin Rattus norvegicus 14-24 2004448-2 1991 CYP from cytoplasmic extracts or erythrocyte lysates was applied in the binding assay with use of [3H]CsA as tracer and charcoal adsorption for separating bound from free tracer. Tritium 99-101 peptidylprolyl isomerase G Homo sapiens 0-3 1900233-5 1991 However, electron immunocytochemical studies of transformed cells indicated the presence of material that cross-reacted with antibodies prepared against the [3H]EBP. Tritium 158-160 EBP, cholestenol delta-isomerase Rattus norvegicus 161-164 1864762-5 1991 EGF specific monoclonal antibody neutralized exogenous EGF-induced 3H-TdR uptake, and inhibited spontaneous 3H-TdR uptake of TMK-1 and MKN-28 cells. Tritium 67-69 epidermal growth factor Homo sapiens 0-3 1864762-5 1991 EGF specific monoclonal antibody neutralized exogenous EGF-induced 3H-TdR uptake, and inhibited spontaneous 3H-TdR uptake of TMK-1 and MKN-28 cells. Tritium 67-69 epidermal growth factor Homo sapiens 55-58 1864762-5 1991 EGF specific monoclonal antibody neutralized exogenous EGF-induced 3H-TdR uptake, and inhibited spontaneous 3H-TdR uptake of TMK-1 and MKN-28 cells. Tritium 108-110 epidermal growth factor Homo sapiens 0-3 1999479-8 1991 In cells labelled with [3H]choline, the water soluble phosphatidylcholine hydrolysis products, phosphorylcholine and choline, were increased 2-fold within 5 minutes of addition of EGF. Tritium 24-26 epidermal growth factor Homo sapiens 180-183 1825248-10 1991 The addition of rIL-2 at the initiation of the MLR was also shown to reverse completely the PRED inhibition of the MLR-induced release of sIL-2R and of the [3H]thymidine incorporation. Tritium 157-159 interleukin 2 Rattus norvegicus 16-21 1674475-4 1991 [Leu5]Enkephalin also reduced [3H]NA release, but higher concentrations were necessary. Tritium 31-33 proenkephalin Rattus norvegicus 6-16 1674475-6 1991 The selective mu opioid receptor agonist, [D-Ala2,NMePhe4,Gly-ol5]enkephalin (DAGO), concentration dependently reduced the stimulus-evoked release of [3H]NA to the same extent as beta-endorphin did. Tritium 151-153 proenkephalin Rattus norvegicus 66-76 1952105-2 1991 The presence of EGF (100 ng/ml) resulted in elevated levels of [3H]-thymidine incorporation when compared to controls; autoradiograms showed that the proliferation of mesenchymal cells in the nephrogenic zone is particularly enhanced as verified by cell counting. Tritium 64-66 epidermal growth factor Mus musculus 16-19 1851764-3 1991 3H-labelled K-elastin was used in order to study elastin--3LL-HM interaction. Tritium 0-2 elastin Mus musculus 14-21 1851764-4 1991 It was found to be saturable (2 ng 3H-labelled K-elastin/10(6) cells), with one class of high-affinity binding sites having Kd equal to 1.3 nM and 16,000 sites/cell. Tritium 35-37 elastin Mus musculus 49-56 2031710-3 1991 Four days after intraventricular injection of kainic acid only a marginal loss of [3H]FOR and a 30% increase of [3H]PDBU binding was seen in the CA1 stratum radiatum while in the CA3 stratum lucidum and radiatum respectively a 30% loss of [3H]FOR and no significant change in [3H]PDBU binding was observed. Tritium 113-115 carbonic anhydrase 1 Rattus norvegicus 145-148 2031710-3 1991 Four days after intraventricular injection of kainic acid only a marginal loss of [3H]FOR and a 30% increase of [3H]PDBU binding was seen in the CA1 stratum radiatum while in the CA3 stratum lucidum and radiatum respectively a 30% loss of [3H]FOR and no significant change in [3H]PDBU binding was observed. Tritium 113-115 carbonic anhydrase 1 Rattus norvegicus 145-148 2031710-3 1991 Four days after intraventricular injection of kainic acid only a marginal loss of [3H]FOR and a 30% increase of [3H]PDBU binding was seen in the CA1 stratum radiatum while in the CA3 stratum lucidum and radiatum respectively a 30% loss of [3H]FOR and no significant change in [3H]PDBU binding was observed. Tritium 113-115 carbonic anhydrase 1 Rattus norvegicus 145-148 1824956-4 1991 The [3H]8-OH-DPAT bindings to the prefrontal cortex and hippocampus were potently inhibited by serotonin (IC50 = 6.3 x 10(-9) M) and 5-HT1A agonists (IC50 = 5.0 x 10(-9) - 2.3 x 10(-7) M), while other neurotransmitters, 5-HT2 and 5-HT3 related compounds did not inhibit the binding (IC50 greater than 10(-5) M). Tritium 5-7 5-hydroxytryptamine receptor 1A Homo sapiens 133-139 1673278-9 1991 Significantly increased neutral proteinase activities against substrates of proteoglycan subunits, [3H]carboxymethylated transferrin and L-pyroglutamyl-L-prolyl-L-valine-paranitroanilide were detected in homogenates of the synovium and cartilage from arthritic knee joints 1 and 2 weeks after induction. Tritium 100-102 inhibitor of carbonic anhydrase Cavia porcellus 121-132 2253202-7 1990 Furthermore, after incubation of CEM-FBP cells for 24 h at 10 nM [3H]ICI-198,583, the high affinity binding of the FBP for ICI-198,583 allowed a 600-fold concentrative uptake of [3H]ICI-198,583 and its conversion to polyglutamate forms. Tritium 66-68 folate receptor alpha Homo sapiens 37-40 2253202-7 1990 Furthermore, after incubation of CEM-FBP cells for 24 h at 10 nM [3H]ICI-198,583, the high affinity binding of the FBP for ICI-198,583 allowed a 600-fold concentrative uptake of [3H]ICI-198,583 and its conversion to polyglutamate forms. Tritium 66-68 folate receptor alpha Homo sapiens 115-118 2253202-7 1990 Furthermore, after incubation of CEM-FBP cells for 24 h at 10 nM [3H]ICI-198,583, the high affinity binding of the FBP for ICI-198,583 allowed a 600-fold concentrative uptake of [3H]ICI-198,583 and its conversion to polyglutamate forms. Tritium 179-181 folate receptor alpha Homo sapiens 37-40 2253202-7 1990 Furthermore, after incubation of CEM-FBP cells for 24 h at 10 nM [3H]ICI-198,583, the high affinity binding of the FBP for ICI-198,583 allowed a 600-fold concentrative uptake of [3H]ICI-198,583 and its conversion to polyglutamate forms. Tritium 179-181 folate receptor alpha Homo sapiens 115-118 1701128-5 1990 Inhibition of IGF-I-stimulated [3H]AIB uptake paralleled the ability of bIGFBP-3 to prevent [125I]IGF-I cell surface binding. Tritium 32-34 insulin like growth factor 1 Bos taurus 14-19 1701128-6 1990 In contrast, preincubation with bIGFBP-3 resulted in a dose-dependent enhancement of IGF-I-stimulated [3H]AIB uptake; a 32-86% increase in IGF-I bioactivity was seen after a 24 h preexposure to 10 nM bIGFBP-3, and a 2- to 6-fold potentiation was seen after a 72 h preincubation. Tritium 103-105 insulin like growth factor 1 Bos taurus 85-90 1704940-3 1990 The 3H-thymidine uptake of cultured human bladder cancer cells, KU-1 and NBT-2, was significantly higher when the cells were treated with 10 ng/ml G-CSF than without G-CSF after 24- and 48-hour incubation. Tritium 4-6 colony stimulating factor 3 Homo sapiens 147-152 2096302-6 1990 [3H]PAF binding studies provided evidence that KO-286011 exerts its inhibitory action at the PAF-receptor level. Tritium 1-3 PCNA clamp associated factor Homo sapiens 4-7 2175183-4 1990 In monolayer cultures of fetal skin fibroblasts both forms of IGFBP-1 inhibited binding of [125I]IGF-I onto the cells, but amplified the IGF-I-stimulated [3H]thymidine incorporation into the same cells. Tritium 155-157 insulin like growth factor binding protein 1 Homo sapiens 62-69 2175183-7 1990 Recently we found that the same IGFBP-1 preparation inhibits IGF-I-stimulated [3H]thymidine incorporation into human hyperstimulated granulosa cells. Tritium 79-81 insulin like growth factor binding protein 1 Homo sapiens 32-39 2228314-4 1990 3H-thymidine incorporation by the LIM1215 cell line was stimulated by low concentrations of epidermal growth factor and basic fibroblast growth factor and, to a lesser extent, by higher concentrations of interleukin-1 and insulin-like growth factor 1. Tritium 0-2 interleukin 1 alpha Homo sapiens 204-250 2211713-7 1990 [3H]Ryanodine-labeled receptor binds to heparin-agarose, and a protein of approximately 400,000 Da, which is cross-reactive with two polyclonal antibodies raised against the skeletal muscle ryanodine receptor, elutes from the column and is enriched in peak [3H]ryanodine binding fractions. Tritium 1-3 ryanodine receptor 1 Oryctolagus cuniculus 174-208 2211713-7 1990 [3H]Ryanodine-labeled receptor binds to heparin-agarose, and a protein of approximately 400,000 Da, which is cross-reactive with two polyclonal antibodies raised against the skeletal muscle ryanodine receptor, elutes from the column and is enriched in peak [3H]ryanodine binding fractions. Tritium 258-260 ryanodine receptor 1 Oryctolagus cuniculus 174-208 2177177-3 1990 PLA2 from bee venom produced a significant increase in the binding of [3H]-AMPA ([3H]-amino-3-hydroxy-5-methylisoxazole-4- propionate), a ligand for the AMPA/quisqualate receptor. Tritium 71-73 phospholipase A2 group IB Rattus norvegicus 0-4 1698553-2 1990 A panel of interleukin 3-dependent cell lines were screened for responsiveness to partially purified MGF in [3H]thymidine incorporation assays; proliferative stimulation of these cells in response to MGF correlated with expression of mRNA for the c-kit protooncogene. Tritium 109-111 kit ligand Mus musculus 101-104 2208585-11 1990 The major product, 13-oxo-9Z,11E-octadecadienoic acid, when instilled intrarectally stimulates the incorporation of [3H]deoxythymidine into colonic mucosal DNA, and induces colonic mucosal ornithine decarboxylase activity in vivo. Tritium 117-119 ornithine decarboxylase 1 Rattus norvegicus 189-212 2124161-6 1990 The inhibitors of phospholipase A2, nordihydroguaiaretic acid and mepacrine, decreased the NMDA-dependent [3H]ARA release in a dose-related manner, maximum inhibition reaching to about 90% at high doses. Tritium 107-109 phospholipase A2 group IB Rattus norvegicus 18-34 2223644-5 1990 The results showed that, in those cases which were good responders to IL-3 in the 3H-TdR uptake assay (19 out of 24), Ara-C exposure eliminated a greater proportion of clonogenic cells if pretreated with IL-3 than if untreated (P less than 0.001), while in cases unresponsive to IL-3 this effect was not significant. Tritium 82-84 interleukin 3 Homo sapiens 70-74 2273455-3 1990 The major pathway for transport of radioactivity into the blood from the nasal cavity appeared to be hydrolysis of [3H]tyr-leucine enkephalin to [3H]L-tyrosine, followed by absorption of [3H]L-tyrosine. Tritium 116-119 proenkephalin Rattus norvegicus 131-141 2273455-3 1990 The major pathway for transport of radioactivity into the blood from the nasal cavity appeared to be hydrolysis of [3H]tyr-leucine enkephalin to [3H]L-tyrosine, followed by absorption of [3H]L-tyrosine. Tritium 116-118 proenkephalin Rattus norvegicus 131-141 2230430-3 1990 As a result, IL-1 suppressed dose-dependently the secretion of both 35S and 3H into the supernatant of the medium containing free human or porcine chondrocytes. Tritium 76-78 interleukin 1 alpha Homo sapiens 13-17 2145093-5 1990 The changes in localization of ir-PCNA during development parallel closely the changes in localization of [3H]thymidine incorporation, reported previously. Tritium 107-109 proliferating cell nuclear antigen Homo sapiens 34-38 2164926-10 1990 In accompanying experiments, 1,25(OH)2[3H]D3 treatment of ROS cells was shown to result in a 3- to 4-fold increased expression of VDR and VDR mRNA. Tritium 39-41 vitamin D receptor Rattus norvegicus 130-133 2164926-10 1990 In accompanying experiments, 1,25(OH)2[3H]D3 treatment of ROS cells was shown to result in a 3- to 4-fold increased expression of VDR and VDR mRNA. Tritium 39-41 vitamin D receptor Rattus norvegicus 138-141 2164926-11 1990 The simultaneous addition of PTH and 1,25(OH)2[3H]D3 resulted in inhibition of the 1,25(OH)2[3H]D3-mediated up-regulation of VDR and VDR mRNA. Tritium 47-49 vitamin D receptor Rattus norvegicus 125-128 2164926-11 1990 The simultaneous addition of PTH and 1,25(OH)2[3H]D3 resulted in inhibition of the 1,25(OH)2[3H]D3-mediated up-regulation of VDR and VDR mRNA. Tritium 47-49 vitamin D receptor Rattus norvegicus 133-136 2164926-11 1990 The simultaneous addition of PTH and 1,25(OH)2[3H]D3 resulted in inhibition of the 1,25(OH)2[3H]D3-mediated up-regulation of VDR and VDR mRNA. Tritium 93-95 vitamin D receptor Rattus norvegicus 125-128 2164926-11 1990 The simultaneous addition of PTH and 1,25(OH)2[3H]D3 resulted in inhibition of the 1,25(OH)2[3H]D3-mediated up-regulation of VDR and VDR mRNA. Tritium 93-95 vitamin D receptor Rattus norvegicus 133-136 2384445-7 1990 By omitting either epidermal growth factor, insulin, or cholera toxin from the medium, pronounced reduction in sizes of islets and in [3H]thymidine incorporation was found. Tritium 135-137 epidermal growth factor Mus musculus 19-42 2140570-3 1990 The analysis of the O-linked saccharides of immunopurified leukosialin, the major [3H]glucosamine-labeled glycoprotein in Jurkat cell lysate, revealed the presence of mainly GalNAc alpha 1----Ser/Thr with only minor amounts (approximately 17%) of more complex O-glycans. Tritium 83-85 LOC105369247 Homo sapiens 59-70 2168782-3 1990 Additionally, the affinities and densities of [3H]NPY binding sites were compared in the temporal cortex, hippocampus and putamen of patients dying from Alzheimer"s disease (AD) and aged matched controls. Tritium 47-49 neuropeptide Y Homo sapiens 50-53 2168782-4 1990 High densities of [3H]NPY binding sites were found in the putamen (192 +/- 32 fmol/mg protein), followed by the hippocampus (165 +/- 42 fmol/mg protein) and temporal cortex (118 +/- 19 fmol/mg protein). Tritium 19-21 neuropeptide Y Homo sapiens 22-25 2340300-4 1990 When CEL was also added, the rate of disappearance of [3H]- and [14C]triacylglycerols increased and the radioactivity of diacylglycerols decreased markedly. Tritium 55-57 carboxyl ester lipase Homo sapiens 5-8 1691278-6 1990 The inhibitory effects of HA-966 and of AP-5 on glutamate-induced [3H]TCP binding were overcome when glutamate concentrations were increased. Tritium 67-69 adaptor related protein complex 5 subunit beta 1 Homo sapiens 40-44 2187189-6 1990 Insulin, as well as IGF-I treatment of diabetic rats increased [3H]thymidine incorporation by thymocytes. Tritium 64-66 insulin-like growth factor 1 Rattus norvegicus 20-25 2346722-6 1990 IL-1 alpha, IL-1 beta and TNF-alpha enhanced 3H-TdR uptake in myeloma cells through IL-6, as antibodies to IL-6 completely abolished the DNA synthesis induced by culture supernatants of MC exposed to these cytokines. Tritium 45-47 interleukin 1 alpha Homo sapiens 0-10 2155218-2 1990 Undifferentiated and differentiated HL-60 cells each exhibit a high capacity to incorporate and metabolize [3H]PAF at 37 degrees C; however, the amount of [3H]PAF that is assimilated by both cell populations is greatly reduced and its metabolism abolished at less than or equal to 4 degrees C. At 0 degrees C HL-60 granulocytes bind more [3H]PAF than their undifferentiated counterparts. Tritium 108-110 PCNA clamp associated factor Homo sapiens 111-114 2155218-2 1990 Undifferentiated and differentiated HL-60 cells each exhibit a high capacity to incorporate and metabolize [3H]PAF at 37 degrees C; however, the amount of [3H]PAF that is assimilated by both cell populations is greatly reduced and its metabolism abolished at less than or equal to 4 degrees C. At 0 degrees C HL-60 granulocytes bind more [3H]PAF than their undifferentiated counterparts. Tritium 156-158 PCNA clamp associated factor Homo sapiens 159-162 2155218-2 1990 Undifferentiated and differentiated HL-60 cells each exhibit a high capacity to incorporate and metabolize [3H]PAF at 37 degrees C; however, the amount of [3H]PAF that is assimilated by both cell populations is greatly reduced and its metabolism abolished at less than or equal to 4 degrees C. At 0 degrees C HL-60 granulocytes bind more [3H]PAF than their undifferentiated counterparts. Tritium 156-158 PCNA clamp associated factor Homo sapiens 159-162 2155218-2 1990 Undifferentiated and differentiated HL-60 cells each exhibit a high capacity to incorporate and metabolize [3H]PAF at 37 degrees C; however, the amount of [3H]PAF that is assimilated by both cell populations is greatly reduced and its metabolism abolished at less than or equal to 4 degrees C. At 0 degrees C HL-60 granulocytes bind more [3H]PAF than their undifferentiated counterparts. Tritium 156-158 PCNA clamp associated factor Homo sapiens 159-162 2155218-2 1990 Undifferentiated and differentiated HL-60 cells each exhibit a high capacity to incorporate and metabolize [3H]PAF at 37 degrees C; however, the amount of [3H]PAF that is assimilated by both cell populations is greatly reduced and its metabolism abolished at less than or equal to 4 degrees C. At 0 degrees C HL-60 granulocytes bind more [3H]PAF than their undifferentiated counterparts. Tritium 156-158 PCNA clamp associated factor Homo sapiens 159-162 2155218-3 1990 Binding to differentiated cells reaches equilibrium within 80 min and is saturable, reversible and specific; PAF receptor antagonists WEB 2086, L-659,989, BN 52021, and kadsurenone abolish this specific [3H]PAF binding. Tritium 204-206 PCNA clamp associated factor Homo sapiens 109-112 2155218-3 1990 Binding to differentiated cells reaches equilibrium within 80 min and is saturable, reversible and specific; PAF receptor antagonists WEB 2086, L-659,989, BN 52021, and kadsurenone abolish this specific [3H]PAF binding. Tritium 204-206 PCNA clamp associated factor Homo sapiens 207-210 2155218-4 1990 In contrast, [3H]PAF uptake by undifferentiated HL-60 cells is neither saturable nor sensitive to specific receptor antagonists. Tritium 14-16 PCNA clamp associated factor Homo sapiens 17-20 2155231-8 1990 Using the AML 193 [3H]thymidine incorporation assay the specific activity of the heavily glycosylated hGM-CSF was 1 x 10(8) units/mg compared with 6 x 10(8) units/mg for the non-glycosylated hGM-CSF produced by Escherichia coli. Tritium 19-21 colony stimulating factor 2 Homo sapiens 102-109 2158857-3 1990 Membrane homogenates from the prenatal stress group showed less binding of the mu opiate receptor ligand, [3H]DAGO in striatum but not in several other brain regions. Tritium 107-109 opioid receptor mu 1 Homo sapiens 79-97 2322898-2 1990 Bifemelane, a drug that augments the long-term potentiation in the CA3 region, significantly induced the translocation of [3H]phorbol 12,13-dibutyrate binding activity from the cytosol to the membrane in a dose-dependent manner (10(-8) to 10(-6) M) and with no effects on total binding activity in the CA3 region. Tritium 123-125 carbonic anhydrase 3 Cavia porcellus 67-70 2322898-2 1990 Bifemelane, a drug that augments the long-term potentiation in the CA3 region, significantly induced the translocation of [3H]phorbol 12,13-dibutyrate binding activity from the cytosol to the membrane in a dose-dependent manner (10(-8) to 10(-6) M) and with no effects on total binding activity in the CA3 region. Tritium 123-125 carbonic anhydrase 3 Cavia porcellus 302-305 2159859-2 1990 Using soluble 3H-elastin as substrate in a cell culture assay we examined the ability of live, adherent human blood neutrophils and monocytes to release elastolytic activity following immune complex (IC) stimulation. Tritium 14-16 elastin Homo sapiens 17-24 2314388-10 1990 As previously reported for the MAO-B ligands [3H]Ro 16-6491 and [3H]Ro 19-6327, the analysis of the membrane-bound radioactivity showed that [3H]Ro 41-1049 was entirely recovered in the form of its aldehyde derivative, indicating that Ro 41-1049 was deaminated by MAO-A. Tritium 46-48 monoamine oxidase B Homo sapiens 31-36 2314388-10 1990 As previously reported for the MAO-B ligands [3H]Ro 16-6491 and [3H]Ro 19-6327, the analysis of the membrane-bound radioactivity showed that [3H]Ro 41-1049 was entirely recovered in the form of its aldehyde derivative, indicating that Ro 41-1049 was deaminated by MAO-A. Tritium 65-67 monoamine oxidase B Homo sapiens 31-36 2314388-10 1990 As previously reported for the MAO-B ligands [3H]Ro 16-6491 and [3H]Ro 19-6327, the analysis of the membrane-bound radioactivity showed that [3H]Ro 41-1049 was entirely recovered in the form of its aldehyde derivative, indicating that Ro 41-1049 was deaminated by MAO-A. Tritium 65-67 monoamine oxidase B Homo sapiens 31-36 1967632-2 1990 [3H]CNQX binding was regionally distributed, with the highest levels of binding present in hippocampus in the stratum radiatum of CA1, stratum lucidum of CA3, and molecular layer of dentate gyrus. Tritium 1-3 carbonic anhydrase 3 Rattus norvegicus 154-157 1968295-3 1990 In nine 7-day-old rats, an acute focal hypoxic-ischemic insult produced by unilateral carotid artery ligation and subsequent exposure to 8% oxygen acutely reduced 3H-TCP binding ipsilateral to the ligation by 30% in the CA1, by 27% in the CA3, by 26% in the dentate gyrus, and by 17% in the striatum compared with values from the contralateral hemisphere. Tritium 163-165 carbonic anhydrase 3 Rattus norvegicus 239-242 2297358-0 1990 Characterization of [3H]Ro 16-6491 binding to digitonin solubilized monoamine oxidase-B and purification of the enzyme from human platelets by affinity chromatography. Tritium 21-23 monoamine oxidase B Homo sapiens 68-87 2225235-5 1990 Twenty-four h after a single injection of CCl4, the intensity of non-polar I-spots in liver DNA was significantly increased as compared with corn oil treated controls, while the level of one polar I-compound was reduced at 24 h. DNA synthesis (as indicated by [3H]thymidine incorporation) was not significantly affected at 24 h after a single dose of CCl4. Tritium 261-263 chemokine (C-C motif) ligand 4 Mus musculus 42-46 2177712-2 1990 The effect of neuropeptide Y (NPY) on [3H]noradrenaline ([3H]NA) release-evoked by 5-Hz electrical stimulation or 5 microns calcium ionophore A23187 was studied in vitro in guinea-pig vas deferens. Tritium 38-41 pro-neuropeptide Y Cavia porcellus 30-33 2177712-6 1990 NPY, 1 microM, reduced electrically-evoked tritium overflow. Tritium 43-50 pro-neuropeptide Y Cavia porcellus 0-3 2177712-10 1990 NPY, 1 microM, reduced A23187-evoked tritium overflow by 52.3 +/- 7.1%. Tritium 37-44 pro-neuropeptide Y Cavia porcellus 0-3 2177712-13 1990 It is concluded that in guinea-pig vas deferens NPY reduces [3H]NA release through affecting adenylate cyclase and the processes responsible for calcium mobilization. Tritium 61-63 pro-neuropeptide Y Cavia porcellus 48-51 2179160-6 1990 The insulin-associated response of stimulated [3H]AIB uptake was induced in MDCK cells by the insulinomimetic lectins concanavalin A (130-140% of basal value at concentrations of 10-40 micrograms/ml) and wheat germ agglutinin (140-160% of basal value at concentrations of 10-30 micrograms/ml). Tritium 47-49 insulin Canis lupus familiaris 4-11 2222781-2 1990 With this in mind we studied platelet monoamine oxidase B (MAO B) activity and 3H-imipramine (IMI) binding in both AD patients and healthy subjects and found a significantly higher level of platelet MAO B activity and 3H-IMI Bmax values in the AD patients. Tritium 79-81 monoamine oxidase B Homo sapiens 199-204 1967647-6 1990 The binding of [3H]RX821002 on the human fat cell alpha-2 adrenoceptor displayed a specificity which is strictly similar to that obtained with [3H]rauwolscine and which is classical for an alpha-2 A adrenoceptor. Tritium 16-18 adrenoceptor alpha 2A Homo sapiens 189-211 34954655-6 2022 FINDINGS: In placental cells, clinically relevant DTG exposure for 3h or 6h was associated with a modest but significant reduction in the expression of RFC and PCFT both at the mRNA and protein levels, as well as decreased uptake of RFC and PCFT substrates (3H)-methotrexate and (3H)-folic acid, respectively. Tritium 67-69 solute carrier family 46, member 1 Mus musculus 160-164 34954655-6 2022 FINDINGS: In placental cells, clinically relevant DTG exposure for 3h or 6h was associated with a modest but significant reduction in the expression of RFC and PCFT both at the mRNA and protein levels, as well as decreased uptake of RFC and PCFT substrates (3H)-methotrexate and (3H)-folic acid, respectively. Tritium 67-69 solute carrier family 46, member 1 Mus musculus 241-245 34954655-6 2022 FINDINGS: In placental cells, clinically relevant DTG exposure for 3h or 6h was associated with a modest but significant reduction in the expression of RFC and PCFT both at the mRNA and protein levels, as well as decreased uptake of RFC and PCFT substrates (3H)-methotrexate and (3H)-folic acid, respectively. Tritium 258-260 solute carrier family 46, member 1 Mus musculus 241-245 34774621-2 2022 MAIN METHODS: NHE1 expressed in native rat tissues or in heterologous cells was assessed for palmitoylation by acyl-biotinyl exchange (ABE) and metabolic labeling with (3H)palmitate. Tritium 169-171 solute carrier family 9 member A1 Rattus norvegicus 14-18 34651220-9 2022 RESULTS: In ARG studies, binding of (3H)T-008 was specific to CH24H in the mouse brain sections, which was not observed in CH24H KO or in wild-type mice after pretreatment with soticlestat. Tritium 37-39 cytochrome P450, family 46, subfamily a, polypeptide 1 Mus musculus 62-67 34248642-5 2021 The results imply that koumine might be a high-affinity ligand of TSPO and that it likely acts as a PAM since it could delay the dissociation of 3H-PK11195 from TSPO. Tritium 145-147 translocator protein Homo sapiens 66-70 34248642-5 2021 The results imply that koumine might be a high-affinity ligand of TSPO and that it likely acts as a PAM since it could delay the dissociation of 3H-PK11195 from TSPO. Tritium 145-147 translocator protein Homo sapiens 161-165 34220834-5 2021 Methods: Lp-PLA2 activity in serum samples collected from 50 primary SS patients with no lymphoma (SS-nL), 9 primary SS patients with lymphoma (SS-L), and 42 healthy controls (HC) was determined by detection of (3H)PAF degradation products by liquid scintillation counter. Tritium 212-214 phospholipase A2 group VII Homo sapiens 9-16 34388064-2 2020 Corneas given a circular transcorneal freeze injury, were explanted into organ cultures containing either insulin or IGF-2 and cultured up to72 h. Both factors increased (3H)-thymidine incorporation and mitotic levels compared to controls. Tritium 171-173 insulin-like growth factor 2 Rattus norvegicus 117-122 35594964-3 2022 Further modifications with fatty acids resulted in novel metabolically stable peptides, among which 3d and 3h showed potent GLP-1R and CCK-2R activation potencies and comparable stability to semaglutide. Tritium 107-109 glucagon-like peptide 1 receptor Mus musculus 124-130 35594964-3 2022 Further modifications with fatty acids resulted in novel metabolically stable peptides, among which 3d and 3h showed potent GLP-1R and CCK-2R activation potencies and comparable stability to semaglutide. Tritium 107-109 cholecystokinin B receptor Mus musculus 135-141 35547552-7 2022 Although there was no significant correlation between these three assays in the healthy controls, the SI value for <10% (3H)-thymidine proliferation in those with T cell deficiency was compatible with CFSE- and Ki-67-stained lymphocyte percentages, and validated in patients with IL2RG, RAG1, and ZAP70 mutations. Tritium 121-123 zeta chain of T cell receptor associated protein kinase 70 Homo sapiens 297-302 34921739-10 2022 Further study showed that compounds 3h, 3r, and 3s displayed superb antibacterial activity against E. carotorora with the EC50 values of 2.65, 4.24 and 4.29 mug/mL respectively, which were superior to streptomycin sulfate (EC50 = 5.96 mug/mL). Tritium 36-38 thrombopoietin Mus musculus 161-163 34921739-10 2022 Further study showed that compounds 3h, 3r, and 3s displayed superb antibacterial activity against E. carotorora with the EC50 values of 2.65, 4.24 and 4.29 mug/mL respectively, which were superior to streptomycin sulfate (EC50 = 5.96 mug/mL). Tritium 36-38 thrombopoietin Mus musculus 239-241 35350429-7 2022 As a preliminary study to validate this PET target in schizophrenia, S1PR1 protein expression was assessed by receptor autoradiography (ARG) using (3H)CS1P1 and immunohistochemistry (IHC) in the DLPFC from patients with schizophrenia classified as Type 1 or Type 2 based on their DLPFC transcriptomes and from controls. Tritium 148-150 sphingosine-1-phosphate receptor 1 Homo sapiens 69-74 2616045-0 1989 Autoradiographic evidence of [3H]neurotensin binding changes in discrete regions of brain in the rat model of persistent spasmodic dyskinesia induced by iminodipropionitrile. Tritium 30-32 neurotensin Rattus norvegicus 33-44 2611252-7 1989 Excess unlabeled retinol-RBP competed with [3H]retinol-RBP for [3H]retinol delivery to the cells, indicating that RBP delivery of retinol was a saturable and competable process. Tritium 44-46 retinol binding protein 4 Homo sapiens 55-58 2611252-7 1989 Excess unlabeled retinol-RBP competed with [3H]retinol-RBP for [3H]retinol delivery to the cells, indicating that RBP delivery of retinol was a saturable and competable process. Tritium 44-46 retinol binding protein 4 Homo sapiens 55-58 2611252-7 1989 Excess unlabeled retinol-RBP competed with [3H]retinol-RBP for [3H]retinol delivery to the cells, indicating that RBP delivery of retinol was a saturable and competable process. Tritium 64-66 retinol binding protein 4 Homo sapiens 25-28 2611252-7 1989 Excess unlabeled retinol-RBP competed with [3H]retinol-RBP for [3H]retinol delivery to the cells, indicating that RBP delivery of retinol was a saturable and competable process. Tritium 64-66 retinol binding protein 4 Homo sapiens 55-58 2611252-7 1989 Excess unlabeled retinol-RBP competed with [3H]retinol-RBP for [3H]retinol delivery to the cells, indicating that RBP delivery of retinol was a saturable and competable process. Tritium 64-66 retinol binding protein 4 Homo sapiens 55-58 2631297-4 1989 The Ah receptor binding affinities of the 6-substituted compounds were determined in a series of in vitro competitive binding studies using [3H]TCDD as the radioligand. Tritium 141-143 aryl hydrocarbon receptor Rattus norvegicus 4-15 2481032-3 1989 Arterial blood samples were taken to determine the rhG-CSF concentrations by measuring its activities using a modified [3H]thymidine assay. Tritium 120-122 colony stimulating factor 2 Rattus norvegicus 55-58 2556707-3 1989 When PDE is subjected to limited proteolysis with trypsin, a 3H-methylated fragment of 1000 daltons or less is rapidly removed prior to the degradation of its inhibitory gamma subunit. Tritium 61-63 aldehyde dehydrogenase 7 family member A1 Homo sapiens 5-8 2811368-13 1989 Since this additional binding is found only within the first hour after injection, a difference in binding to AR between brain and accessory sex glands after [3H]T is suggested. Tritium 159-161 androgen receptor Mus musculus 110-112 2479949-5 1989 The binding of [3H]H12-histrionicotoxin to Torpedo membranes was enhanced in the presence of NMTHT indicating an agonist action at this vertebrate peripheral nicotinic acetylcholine receptor. Tritium 16-18 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 158-190 2476404-4 1989 3H thymidine (3H-TdR) incorporation was remarkably inhibited by ADM with pre-treatment of 100 IU/ml of each IFN, and an additive effect of ADM and IFN on cell growth inhibition could be observed. Tritium 0-2 adrenomedullin Mus musculus 139-150 2782436-1 1989 The effect of bradykinin on membrane potential of cultured bovine aortic endothelial cells (BAECs) was estimated by measuring the uptake of the lipophilic cation, tetra[3H]phenylphosphonium ([3H]TPP+). Tritium 169-171 kininogen 1 Bos taurus 14-24 2504156-5 1989 This model system was utilized to study the effects of FSH on the quaternary structure of FSH receptor-associated GTP-binding protein by comparing the gel filtration profiles of proteoliposomes solubilized with Triton X-100 after exposure to [3H]Gpp(NH)p in the presence or absence of FSH. Tritium 243-246 follicle stimulating hormone receptor Homo sapiens 90-102 2737143-3 1989 In cultured calvariae, 0.5-5 nM PTH-rp stimulated [3H]thymidine incorporation into DNA by 25-70% after 24 h of treatment and decreased relative [3H]proline incorporation into collagen by 50%; the inhibitory effect on collagen production was not altered by hydroxyurea, which decreased DNA synthesis by 85%. Tritium 51-53 parathyroid hormone-like hormone Rattus norvegicus 32-38 2737143-3 1989 In cultured calvariae, 0.5-5 nM PTH-rp stimulated [3H]thymidine incorporation into DNA by 25-70% after 24 h of treatment and decreased relative [3H]proline incorporation into collagen by 50%; the inhibitory effect on collagen production was not altered by hydroxyurea, which decreased DNA synthesis by 85%. Tritium 145-147 parathyroid hormone-like hormone Rattus norvegicus 32-38 2737143-4 1989 PTH-rp also increased [3H]hydroxyproline levels by 100% in culture medium from bones prelabeled with [3H]proline, indicating accelerated matrix turnover. Tritium 23-25 parathyroid hormone-like hormone Rattus norvegicus 0-6 2737143-4 1989 PTH-rp also increased [3H]hydroxyproline levels by 100% in culture medium from bones prelabeled with [3H]proline, indicating accelerated matrix turnover. Tritium 102-104 parathyroid hormone-like hormone Rattus norvegicus 0-6 2471732-1 1989 The mouse mast cell line PT-18 demonstrates [3H] thymidine uptake in the presence of either mouse IL-3 or mouse recombinant granulocyte-macrophage CSF (rGM-CSF). Tritium 45-47 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 156-159 2656709-6 1989 As demonstrated by the parallel analysis of immunoprecipitates from periodate-[3H]sodium borohydride-labeled platelets, the [3H]palmitate-labeled bands obtained under reducing conditions corresponded to GP Ib beta and GP IX and the ones obtained under nonreducing conditions to intact GP Ib and GP IX, respectively. Tritium 79-81 glycoprotein Ib platelet subunit beta Homo sapiens 203-213 2668802-0 1989 The effects of N-acetylated alpha-linked acidic dipeptidase (NAALADase) inhibitors on [3H]NAAG catabolism in vivo. Tritium 87-89 folate hydrolase 1B (pseudogene) Homo sapiens 15-59 2668802-0 1989 The effects of N-acetylated alpha-linked acidic dipeptidase (NAALADase) inhibitors on [3H]NAAG catabolism in vivo. Tritium 87-89 folate hydrolase 1B (pseudogene) Homo sapiens 61-70 2668802-3 1989 Co-injections of NAALADase inhibitors, such as quisqualate (Quis), phosphate, dithiothreitol and EGTA were found to prolong the t1/2 of [3H]NAAG, whereas cobalt, a NAALADase activity stimulator, accelerated [3H]NAAG catabolism. Tritium 137-139 folate hydrolase 1B (pseudogene) Homo sapiens 17-26 2668802-3 1989 Co-injections of NAALADase inhibitors, such as quisqualate (Quis), phosphate, dithiothreitol and EGTA were found to prolong the t1/2 of [3H]NAAG, whereas cobalt, a NAALADase activity stimulator, accelerated [3H]NAAG catabolism. Tritium 208-210 folate hydrolase 1B (pseudogene) Homo sapiens 17-26 2497784-1 1989 The 3H-labeled prostaglandin D2 [( 3H]PGD2) binding protein in the membrane fraction of mastocytoma P-815 cells was characterized. Tritium 4-6 prostaglandin D2 synthase (brain) Mus musculus 38-42 2497784-2 1989 The specific binding of [3H]PGD2 to the cells or the membranes reached a maximum at pH 5.6, and was saturable, displaceable and of high affinity when incubated at 0 or 37 degrees C. The Bmax values for [3H]PGD2 binding in the two preparations at pH 5.6 were much higher at 0 degrees C than at 37 degrees C, whereas the Kd values were almost equal (85.3 nM for the cells and 80.5 nM for the membranes, respectively). Tritium 25-27 prostaglandin D2 synthase (brain) Mus musculus 28-32 2497784-2 1989 The specific binding of [3H]PGD2 to the cells or the membranes reached a maximum at pH 5.6, and was saturable, displaceable and of high affinity when incubated at 0 or 37 degrees C. The Bmax values for [3H]PGD2 binding in the two preparations at pH 5.6 were much higher at 0 degrees C than at 37 degrees C, whereas the Kd values were almost equal (85.3 nM for the cells and 80.5 nM for the membranes, respectively). Tritium 25-27 prostaglandin D2 synthase (brain) Mus musculus 206-210 2497784-2 1989 The specific binding of [3H]PGD2 to the cells or the membranes reached a maximum at pH 5.6, and was saturable, displaceable and of high affinity when incubated at 0 or 37 degrees C. The Bmax values for [3H]PGD2 binding in the two preparations at pH 5.6 were much higher at 0 degrees C than at 37 degrees C, whereas the Kd values were almost equal (85.3 nM for the cells and 80.5 nM for the membranes, respectively). Tritium 203-205 prostaglandin D2 synthase (brain) Mus musculus 28-32 2497784-3 1989 High specific [3H]PGD2 binding activity in the mildly acid-treated cells was still observed when the external pH was raised from 5.6 to 7.2. Tritium 15-17 prostaglandin D2 synthase (brain) Mus musculus 18-22 2497784-4 1989 Furthermore, specific [3H]PGD2 binding to the membranes (at 0 degrees C, pH 5.6) increased on addition of phosphatase inhibitors (NaF and molybdate) in the presence of 10 microM ATP, but practically disappeared on pretreatment of the membranes with phosphatase. Tritium 23-25 prostaglandin D2 synthase (brain) Mus musculus 26-30 2497784-6 1989 These results suggest that [3H]PGD2 binding in the mastocytoma P-815 cell membrane is controlled through phosphorylation-dephosphorylation of the receptor itself. Tritium 28-30 prostaglandin D2 synthase (brain) Mus musculus 31-35 2539972-5 1989 The immunocytochemical evidence for colocalization of CAT within the ACTH cell was strengthened by the finding of a significantly higher rate of [3H]ACh synthesis in a corticotroph-enriched cell population obtained by separating pituitary cells on a velocity sedimentation gradient. Tritium 146-148 choline O-acetyltransferase Rattus norvegicus 54-57 2525310-1 1989 The effects of neurotensin in vitro (1-100 nM) on the binding characteristics of [3H]N-propylnorapomorphine ([3H]NPA) were analysed in striatal membrane preparations of the adult male rat. Tritium 82-84 neurotensin Rattus norvegicus 15-26 2525310-2 1989 Subsequently, it was investigated whether the modulatory effects of 10 nM neurotensin on [3H]NPA binding were altered by treatment with toluene in vivo (80 p.p.m., 3 days, 6 h day-1) and in vitro (19 mumol ml-1). Tritium 90-92 neurotensin Rattus norvegicus 74-85 2525310-6 1989 The modulatory effect of neurotensin remained intact also following toluene treatment in vivo and in vitro, although at a higher KD range, since toluene alone increased the KD value of [3H]NPA binding by 40-50%. Tritium 186-188 neurotensin Rattus norvegicus 25-36 2525310-8 1989 When neurotensin and toluene treatments were combined, the KD values of [3H]NPA binding were about twice as high as in non-treated controls. Tritium 73-75 neurotensin Rattus norvegicus 5-16 2539049-5 1989 HCB, at concentrations of 1 microM or higher, inhibited the specific binding of [3H]TCDD (0.3 nM) to the Ah receptor in vitro, whereas the solubility of [3H]TCDD was affected only at 100 microM HCB. Tritium 81-83 aryl hydrocarbon receptor Rattus norvegicus 105-116 2539049-7 1989 In rats fed a diet containing 3000 ppm HCB for varying times (4 h to 7 days), the specific binding of [3H]TCDD in hepatic cytosol was reduced by up to 40%, as observed previously for known Ah receptor agonists. Tritium 103-105 aryl hydrocarbon receptor Rattus norvegicus 189-200 2544916-7 1989 The results support the hypothesis that the mechanisms of the regulation of [3H]serotonin uptake sensitivity to IMI and TRA in patients are different. Tritium 77-79 T cell receptor alpha locus Homo sapiens 120-123 2923910-3 1989 Intravenously administered [3H]retinol bound to retinol binding protein-transthyretin complex (RBP-TTR complex) was used to study the dynamics of circulatory retinol in these rats. Tritium 28-30 transthyretin Rattus norvegicus 99-102 2519887-1 1989 Suspensions of neonatal rat myocytes were used to investigate the metabolism of [3H]PAF (1-alkyl-2-acetyl-(sn-glycero-3-phosphocholine) (GPC] and [3H]alkyllyso-GPC. Tritium 81-83 PCNA clamp associated factor Rattus norvegicus 84-87 2519887-2 1989 [3H]Alkylacyl-GPC consisting of molecular species with four or more double bonds (87%) was the major metabolite formed when either [3H]PAF (4 x 10(-6) - 4 x 10(-9) M) or [3H]alkyllyso-GPC (2 x 10(-7) M) was the precursor. Tritium 1-3 PCNA clamp associated factor Rattus norvegicus 135-138 2519887-3 1989 However, substantial amounts of [3H]alkyllyso-GPC (mostly in the media) were also generated from [3H]PAF during the early periods of the incubations. Tritium 33-35 PCNA clamp associated factor Rattus norvegicus 101-104 2519887-3 1989 However, substantial amounts of [3H]alkyllyso-GPC (mostly in the media) were also generated from [3H]PAF during the early periods of the incubations. Tritium 98-100 PCNA clamp associated factor Rattus norvegicus 101-104 2519887-6 1989 Pretreatment of neonatal rat myocytes with phenylmethylsulfonyl fluoride partially blocked the deacetylation of [3H]PAF and decreased both the formation and subsequent acylation of [3H]alkyllyso-GPC in intact cells. Tritium 113-115 PCNA clamp associated factor Rattus norvegicus 116-119 2519887-9 1989 In addition, when [3H]alkylacetylglycerol was the precursor, 75% of the label appeared as alkylglycerols in the neonatal rat myocytes, but [3H]PAF was not detected. Tritium 19-21 PCNA clamp associated factor Rattus norvegicus 143-146 2522304-1 1989 The cellular phospholipase A2 activity of mouse thymocytes was estimated in vitro by the release of [3H]-Arachidonic acid from labeled and calcium ionophore A23187-stimulated cells. Tritium 101-105 phospholipase A2, group IB, pancreas Mus musculus 13-29 2914273-7 1989 The cellular accumulation of [3H]-daunomycin by HC-7-5 cells was about 70% that of HC-2-6 cells. Tritium 30-32 CYCS pseudogene 40 Homo sapiens 48-52 2730238-2 1989 [3H]-QNB accumulated in the hippocampus of 3 month old rats, primarily in the stratum oriens of the CA1 and CA2 fields, followed in descending order by the molecular layer of granule cells of the dentate gyrus and the CA3 field. Tritium 1-3 carbonic anhydrase 3 Rattus norvegicus 218-221 2730238-4 1989 In contrast, the density of [3H]-QNB binding sites in the hippocampus of 22 months old rats was significantly reduced, mainly in the granule cells of the dentate gyrus, next in the CA3 and in the CA1-CA2 fields. Tritium 29-31 carbonic anhydrase 3 Rattus norvegicus 181-184 2480261-12 1989 Substance P displaced around 50% of [3H]neurotensin in the cerebral cortex. Tritium 37-39 neurotensin Rattus norvegicus 40-51 2913283-2 1989 CCK and gastrin induced the Ca++-dependent and tetrodotoxin-sensitive release of [3H]ACh from the LM-MP preparations preloaded with [3H]choline. Tritium 82-84 cholecystokinin Cavia porcellus 0-3 2913283-2 1989 CCK and gastrin induced the Ca++-dependent and tetrodotoxin-sensitive release of [3H]ACh from the LM-MP preparations preloaded with [3H]choline. Tritium 133-135 cholecystokinin Cavia porcellus 0-3 2696851-13 1989 When aminoglutethimide, a potent inhibitor of cytochrome P-450scc, was added during the incubation of cells with [3H]mevalonolactone, [3H]cholesterol accumulated in the cells. Tritium 114-116 cytochrome P450, family 11, subfamily a, polypeptide 1 Rattus norvegicus 46-65 2536464-6 1989 Rat kidney mitochondria were fractionated by decompression and digitonin-based methods into outer and inner membrane-containing fractions before and after incorporation of the MBR-specific photoaffinity ligand [3H]PK14105. Tritium 211-213 translocator protein Rattus norvegicus 176-179 2536464-8 1989 We also evaluated the binding of a large number of structurally and pharmacologically diverse compounds to MBR by studying their ability to inhibit the binding of both 3H-ligands. Tritium 168-170 translocator protein Rattus norvegicus 107-110 2601836-4 1989 Twenty days after unilateral or bilateral lesion of the nucleus basalis a marked decrease in choline acetyltransferase activity was associated with a decrease in [3H]purine release. Tritium 163-165 choline O-acetyltransferase Rattus norvegicus 93-118 2521959-7 1989 We conclude that the majority of 3H-5-HT labeled recognition sites in human cortex, caudate, and globus pallidus represent 5-HT1D binding sites. Tritium 33-35 5-hydroxytryptamine receptor 1D Homo sapiens 123-129 2849934-1 1988 The ability of bradykinin to stimulate phosphodiesteratic cleavage of phosphatidylcholine (PC) was investigated in bovine pulmonary artery endothelial cells prelabeled with [3H]choline and [3H]myristic acid. Tritium 174-176 kininogen 1 Bos taurus 15-25 2849934-1 1988 The ability of bradykinin to stimulate phosphodiesteratic cleavage of phosphatidylcholine (PC) was investigated in bovine pulmonary artery endothelial cells prelabeled with [3H]choline and [3H]myristic acid. Tritium 190-192 kininogen 1 Bos taurus 15-25 3198596-2 1988 When CRBP carrying [3H]retinol as its ligand was purified from rat liver cytosol and incubated with rat liver microsomes, a significant fraction of the [3H]retinol was converted to [3H]retinyl ester. Tritium 19-22 retinol binding protein 1 Rattus norvegicus 5-9 3198596-3 1988 Esterification of the CRBP-bound [3H]retinol, which was maximal at pH 6-7, did not require the addition of an exogenous fatty acyl group. Tritium 33-36 retinol binding protein 1 Rattus norvegicus 22-26 3198596-5 1988 The esterification reaction had a Km for [3H]retinol-CRBP of 4 +/- 0.6 microM and a maximum velocity of 145 +/- 52 pmol/min/mg of microsomal protein (n = 4). Tritium 42-44 retinol binding protein 1 Rattus norvegicus 53-57 3198596-6 1988 The major products were retinyl palmitate/oleate and retinyl stearate in a ratio of approximately 2 to 1 over a range of [3H]retinol-CRBP concentrations from 1 to 8 microM. Tritium 122-124 retinol binding protein 1 Rattus norvegicus 133-137 2846697-3 1988 In Lettre cells, damage is restricted and reversible: little lactate dehydrogenase leaks from cells that leak substantial amounts of Na+, K+, and phosphoryl[3H]choline; at low amounts of cytolysin, membrane potential and intracellular content of Na+ and K+ recover within minutes. Tritium 157-159 Lactate dehydrogenase Drosophila melanogaster 61-82 3141589-2 1988 [3H]5-HT binding, in the presence of pindolol (to block 5-HT1A and 5-HT1B receptors) and mesulergine (to block 5-HT1C receptors), was specific, saturable, reversible, and of high affinity. Tritium 1-3 5-hydroxytryptamine receptor 2C Homo sapiens 111-117 3264358-8 1988 The first component, that was saturated at 5 nM of [3H]R1881, was due to AR while the other two binding components, showing positive-cooperativity (Hill coefficients of 1.90 and 5.07, respectively), were saturated at concentrations of 15 and 30 nM of [3H]R1881. Tritium 52-54 androgen receptor Canis lupus familiaris 73-75 3216867-7 1988 Antisera reacted with high titer against the synthetic peptide by enzyme-linked immunosorbent assay and against the native [3H]dihydrotestosterone-labeled androgen receptor as evidenced by an increase in receptor sedimentation rate determined by sucrose gradient centrifugation. Tritium 124-126 androgen receptor Rattus norvegicus 155-172 3196741-3 1988 PAF was quantified by bioassay, its ability to cause [3H]serotonin release from washed rabbit platelets. Tritium 54-56 PCNA clamp associated factor Rattus norvegicus 0-3 2972704-9 1988 When the cells were labeled with [3H] leucine and the secreted N-terminal ANF-related material was immunoprecipitated and analyzed by tryptic mapping, it was shown to possess labeled tryptic peptides consistent with the structure of ANF-(1-98). Tritium 34-36 natriuretic peptide A Rattus norvegicus 74-77 2972704-10 1988 Tryptic mapping of [3H]arginine-labeled N-terminal ANF-related material demonstrated the presence of all peptides consistent with the ANF-(1-98) structure, including ANF-(92-98). Tritium 20-22 natriuretic peptide A Rattus norvegicus 51-54 2844393-8 1988 Although the drug resistance marker Mr 180,000 glycoprotein could not be detected in any of our cell lines, cellular accumulation of [3H]VP-16 was reduced 50-75% in the resistant lines compared with parent KB. Tritium 134-136 host cell factor C1 Homo sapiens 137-142 2973322-5 1988 Kinetic analysis for [3H]U46619 binding to the membrane fractions in the presence of 20 mM MgCl2 gave a K1 of 6.9 x 10(6) M-1 min-1 and a K-1 of 0.25 min-1, yielding a Kd (K-1/K1) of 36 nM; the value corresponded well to Kd values from Scatchard analysis in both intact (37 nM) and crude membrane fractions (39 nM). Tritium 22-24 keratin 1 Rattus norvegicus 172-178 3421992-5 1988 The FAD containing subunits corresponding to MAO A and MAO B, previous binding of the enzyme with [3H]pargyline and posterior SDS electrophoresis and fluorography, showed molecular weights of 65,900 and 62,400, respectively, in both cellular fractions. Tritium 99-101 monoamine oxidase B Rattus norvegicus 55-60 2848203-6 1988 In the pulmonary artery the angiotensin receptor agonist Val5-angiotensin II-Asp1-beta-amide also increased the electrically evoked tritium overflow in a concentration-dependent manner. Tritium 132-139 beta-secretase 2 Homo sapiens 77-81 3208103-2 1988 Lesioning the CA3 subfield by intracerebroventricular kainic acid injection caused a 50% reduction in the maximal binding capacity of [3H]CHA in the CA3 subfield with no alteration in binding affinity. Tritium 135-137 carbonic anhydrase 3 Rattus norvegicus 14-17 3208103-2 1988 Lesioning the CA3 subfield by intracerebroventricular kainic acid injection caused a 50% reduction in the maximal binding capacity of [3H]CHA in the CA3 subfield with no alteration in binding affinity. Tritium 135-137 carbonic anhydrase 3 Rattus norvegicus 149-152 3208103-3 1988 Five days after unilateral CA3 lesioning, a reduction of [3H]CHA binding by 10-30% of ipsi- and contralateral was observed in the dendritic fields in the CA1 region, though a significant reduction was restricted to the ipsilateral stratum radiatum. Tritium 58-60 carbonic anhydrase 3 Rattus norvegicus 27-30 3041914-4 1988 With both [3H]myristate and [3H]palmitate, Cleveland maps from immunoprecipitates of the transferrin receptor labeled in intact cells and isolated membranes show that identical peptides are labeled. Tritium 11-13 transferrin receptor protein 1 Ovis aries 89-109 3041914-4 1988 With both [3H]myristate and [3H]palmitate, Cleveland maps from immunoprecipitates of the transferrin receptor labeled in intact cells and isolated membranes show that identical peptides are labeled. Tritium 29-31 transferrin receptor protein 1 Ovis aries 89-109 3178736-4 1988 After an initial lag of 30 min, the release of [3H]lysophosphatidylcholine was linear for at least 4 h. At low concentrations, albumin slightly stimulated [3H]phosphatidylcholine release. Tritium 48-50 albumin Rattus norvegicus 127-134 2840079-13 1988 From binding studies with [3H]neurotensin, we found that both the apparent equilibrium dissociation constant, KD, and the maximum number of receptor sites, Bmax, for this radioligand were decreased significantly (P less than 0.05) for completely desensitized cells from those values for control cells. Tritium 27-29 neurotensin Mus musculus 30-41 3181834-3 1988 When cells were incubated with [3H]T-2 a significant increase in the quantity of T-2 associated with the cell occurred during the first 30 min, this increased further from 10-16 hr, and decreased after 24 hr. Tritium 32-34 solute carrier family 25 member 5 Homo sapiens 35-38 3181834-3 1988 When cells were incubated with [3H]T-2 a significant increase in the quantity of T-2 associated with the cell occurred during the first 30 min, this increased further from 10-16 hr, and decreased after 24 hr. Tritium 32-34 solute carrier family 25 member 5 Homo sapiens 81-84 3417113-1 1988 Specific [3H]estradiol-17 beta ([3H]E2) binding activity (EBA) with characteristics of an estrogen receptor (ER) was demonstrated in cytosols and nuclear extracts of the female turtle, Chrysemys picta. Tritium 10-12 estrogen receptor Chrysemys picta 90-107 3417113-1 1988 Specific [3H]estradiol-17 beta ([3H]E2) binding activity (EBA) with characteristics of an estrogen receptor (ER) was demonstrated in cytosols and nuclear extracts of the female turtle, Chrysemys picta. Tritium 10-12 estrogen receptor Chrysemys picta 109-111 3380784-4 1988 The most noticeable change was observed in the increased activity of specific protein-tyrosine kinases and protein kinase C and an increased level of p21ras-associated binding of [3H]GTP. Tritium 180-182 Harvey rat sarcoma virus oncogene Mus musculus 150-156 3377791-1 1988 [3H]RU 486 competes with dexamethasone for rat kidney glucocorticoid receptor (GR) occupancy in vitro, exhibiting a higher association constant for binding to GR than [3H] dexamethasone. Tritium 1-3 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 54-77 3377791-1 1988 [3H]RU 486 competes with dexamethasone for rat kidney glucocorticoid receptor (GR) occupancy in vitro, exhibiting a higher association constant for binding to GR than [3H] dexamethasone. Tritium 1-3 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 79-81 3377791-1 1988 [3H]RU 486 competes with dexamethasone for rat kidney glucocorticoid receptor (GR) occupancy in vitro, exhibiting a higher association constant for binding to GR than [3H] dexamethasone. Tritium 1-3 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 159-161 3377791-3 1988 Interestingly, sulfhydryl reagents such as N-ethylmaleimide, iodoacetamide and tosyllysyl chloromethane at 5mM concentration almost completely inhibited binding of [3H]dexamethasone to GR, whereas 20-30% binding to [3H]RU 486 was inhibited by these reagents. Tritium 165-167 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 185-187 3377791-3 1988 Interestingly, sulfhydryl reagents such as N-ethylmaleimide, iodoacetamide and tosyllysyl chloromethane at 5mM concentration almost completely inhibited binding of [3H]dexamethasone to GR, whereas 20-30% binding to [3H]RU 486 was inhibited by these reagents. Tritium 216-218 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 185-187 2835360-7 1988 These studies were facilitated by the development of a novel tritiated GRP-based radioligand, [3H-Phe15] GRP-15-27, which exhibits enhanced chemical stability compared to iodinated GRP derivatives. Tritium 95-97 gastrin releasing peptide Homo sapiens 71-74 2835360-7 1988 These studies were facilitated by the development of a novel tritiated GRP-based radioligand, [3H-Phe15] GRP-15-27, which exhibits enhanced chemical stability compared to iodinated GRP derivatives. Tritium 95-97 gastrin releasing peptide Homo sapiens 105-108 2835360-7 1988 These studies were facilitated by the development of a novel tritiated GRP-based radioligand, [3H-Phe15] GRP-15-27, which exhibits enhanced chemical stability compared to iodinated GRP derivatives. Tritium 95-97 gastrin releasing peptide Homo sapiens 105-108 2898111-7 1988 Cholecystokinin, angiotensin II, vasopressin, thyrotropin-releasing hormone and bombesin also stimulated [3H]IP1 production but were generally much weaker than BK. Tritium 106-108 gastrin releasing peptide Homo sapiens 80-88 3126263-0 1988 [3H]Ro 16-6491, a selective probe for affinity labelling of monoamine oxidase type B in human brain and platelet membranes. Tritium 1-3 monoamine oxidase B Homo sapiens 60-84 3126263-1 1988 [3H]Ro 16-6491 [N-(2-aminoethyl)-p-chlorobenzamide HCl], a reversible "mechanism-based" inhibitor of monoamine oxidase (MAO) type B, binds selectively and with high affinity to the active site of MAO-B in brain and platelet membranes. Tritium 1-3 monoamine oxidase B Homo sapiens 101-131 3126263-1 1988 [3H]Ro 16-6491 [N-(2-aminoethyl)-p-chlorobenzamide HCl], a reversible "mechanism-based" inhibitor of monoamine oxidase (MAO) type B, binds selectively and with high affinity to the active site of MAO-B in brain and platelet membranes. Tritium 1-3 monoamine oxidase B Homo sapiens 196-201 2837399-7 1988 The results indicate that [3H]PK 14105 identifies the ligand binding domain of the peripheral-type benzodiazepine receptor, which is a peptide with Mr = 18,500, that is of similar size in kidney, heart, brain and adrenals. Tritium 27-29 translocator protein Rattus norvegicus 83-122 2837400-4 1988 We also describe here a non-GABA-associated [3H]SR 95531 binding site which is found at its highest level in the hippocampal CA1 region but also in CA2, CA3, dentate gyrus and cerebral cortex. Tritium 45-47 carbonic anhydrase 3 Rattus norvegicus 153-156 2966310-1 1988 In human caudate and cortex membranes, [3H]serotonin ([3H]5-HT) labels 5-HT1A and 5-HT1C recognition sites which show nanomolar affinity for 8-OH-DPAT (8-hydroxy-2-(di-n-propylamino)-tetralin) and mesulergine respectively, whereas no 5-HT1B binding could be identified. Tritium 40-42 5-hydroxytryptamine receptor 2C Homo sapiens 82-88 2966310-1 1988 In human caudate and cortex membranes, [3H]serotonin ([3H]5-HT) labels 5-HT1A and 5-HT1C recognition sites which show nanomolar affinity for 8-OH-DPAT (8-hydroxy-2-(di-n-propylamino)-tetralin) and mesulergine respectively, whereas no 5-HT1B binding could be identified. Tritium 55-57 5-hydroxytryptamine receptor 2C Homo sapiens 82-88 3132026-2 1988 It was found that all tested benzodiazepines suppressed [3H]-thymidine incorporation into the DNA of glioma cells, the effects being stronger in case of peripheral-type benzodiazepine receptor ligands. Tritium 57-59 translocator protein Homo sapiens 153-192 3346718-3 1988 Exogenous GM1, but not asialo-GM1, sialic acid, or the oligosaccharide chain of GM1, enhances in a time- and concentration-dependent manner the specific 3H-dopamine uptake (increase of the apparent Vmax and decrease of the apparent Km value) and the long-term survival of the dopaminergic neurons. Tritium 153-155 coenzyme Q10A Mus musculus 10-13 3162318-3 1988 Compounds I and II were competitive inhibitors of [3H]Boc[Ahx28,31]CCK-(27-33) binding to central CCK receptors and showed a high degree of selectivity for these binding sites (compound I: Ki for pancreas/Ki for brain, 179; compound II: Ki for pancreas/Ki for brain, 1979). Tritium 51-53 cholecystokinin Cavia porcellus 67-70 3162318-3 1988 Compounds I and II were competitive inhibitors of [3H]Boc[Ahx28,31]CCK-(27-33) binding to central CCK receptors and showed a high degree of selectivity for these binding sites (compound I: Ki for pancreas/Ki for brain, 179; compound II: Ki for pancreas/Ki for brain, 1979). Tritium 51-53 cholecystokinin Cavia porcellus 98-101 3341763-7 1988 Upon elution of the material from the polycrylamide gels, a 16 kDa moiety was found to be more reproducibly active in the MGSA [3H]thymidine bioassay and enzyme-linked immunoadsorbent assay. Tritium 128-130 C-X-C motif chemokine ligand 1 Homo sapiens 122-126 3073912-1 1988 The KRAS2 gene was localized in the rabbit by chromosomal in situ hybridization, using a 3H-labeled human cDNA probe. Tritium 89-91 KRAS proto-oncogene, GTPase Homo sapiens 4-9 3343704-4 1988 In cerebellum and striatum of reeler mice, (3H)DA binding increases 125-174% and 14%, respectively. Tritium 44-46 reelin Mus musculus 30-36 2828800-1 1988 The in vitro and in vivo regulation of [3H]Ro 5-4864 binding to peripheral benzodiazepine receptors (PBR) by ion transport/exchange inhibitors was studied in the kidney. Tritium 40-42 translocator protein Rattus norvegicus 64-99 2828800-1 1988 The in vitro and in vivo regulation of [3H]Ro 5-4864 binding to peripheral benzodiazepine receptors (PBR) by ion transport/exchange inhibitors was studied in the kidney. Tritium 40-42 translocator protein Rattus norvegicus 101-104 3352865-3 1988 The 5-HT1B compounds, mCPP and quipazine were more potent than exogenous 5-HT at decreasing K+-evoked release of [3H]5-HT in slices of spinal cord. Tritium 114-116 5-hydroxytryptamine receptor 1B Rattus norvegicus 4-10 3352865-4 1988 The pEC40 values of 5-HT agonists tested, determined from release assays, significantly correlated with the relative affinities (pKD"s) of these compounds for the binding of [3H]5-HT to the 5-HT1B receptor subtype in the presence of 2 microM 8-OHDPAT, as determined from radioligand binding studies (r = 0.98, P = 0.003). Tritium 175-177 5-hydroxytryptamine receptor 1B Rattus norvegicus 190-196 2450306-0 1987 Cholecystokinin releases [3H]GABA from the perfused subarachnoid space of the anaesthetized rat spinal cord. Tritium 26-28 cholecystokinin Rattus norvegicus 0-15 2450306-3 1987 Low doses of CCK-8 (1 nM) have been found to evoke calcium-dependent [3H]GABA release from an in vivo perfused spinal cord preparation in the anaesthetized rat. Tritium 70-72 cholecystokinin Rattus norvegicus 13-16 2450306-4 1987 Tachyphylaxis was seen to the [3H]GABA releasing action of CCK-8. Tritium 31-33 cholecystokinin Rattus norvegicus 59-62 2450306-6 1987 totally blocked the [3H]GABA release produced by CCK-8 or by a medium containing 50 mM potassium. Tritium 21-23 cholecystokinin Rattus norvegicus 49-52 2829989-3 1987 Twenty-one days after injection of kainate in the caudate putamen (CP), the NEP level was locally decreased (52%) but the time course of this decrease was different from that of mu and delta opioid receptors: [3H]DAGO binding was diminished by 40% from day 2 whereas that of [3H]DTLET was reduced by 51% from day 7. Tritium 210-212 membrane metallo-endopeptidase Rattus norvegicus 76-79 2829989-3 1987 Twenty-one days after injection of kainate in the caudate putamen (CP), the NEP level was locally decreased (52%) but the time course of this decrease was different from that of mu and delta opioid receptors: [3H]DAGO binding was diminished by 40% from day 2 whereas that of [3H]DTLET was reduced by 51% from day 7. Tritium 276-278 membrane metallo-endopeptidase Rattus norvegicus 76-79 3678131-9 1987 Using simultaneous histochemical staining for alkaline phosphatase activity and [3H]thymidine nuclear uptake we found that aFGF stimulates DNA synthesis to the same extent in alkaline phosphatase-rich (osteoblastic) and alkaline phosphatase-poor (nonosteoblastic) cells. Tritium 81-83 fibroblast growth factor 1 Rattus norvegicus 123-127 2445600-4 1987 Reduction of the intracellular cyclic AMP with 2"-deoxyadenosine or a cyclic GMP elevation with its dibutyryl derivative, or liposomes containing cyclic GMP stimulated the uptake of [3H]thymidine and protein synthesis in the cells cultured from unaffected intima. Tritium 183-185 5'-nucleotidase, cytosolic II Homo sapiens 77-80 2445600-4 1987 Reduction of the intracellular cyclic AMP with 2"-deoxyadenosine or a cyclic GMP elevation with its dibutyryl derivative, or liposomes containing cyclic GMP stimulated the uptake of [3H]thymidine and protein synthesis in the cells cultured from unaffected intima. Tritium 183-185 5'-nucleotidase, cytosolic II Homo sapiens 153-156 3681301-3 1987 In experiments measuring the time course of [3H]neurotensin degradation, a minimum of six major tritiated products were found, with the breakdown peptides formed and the degree of proteolysis of [3H]neurotensin being dependent upon the length of incubation and the concentration of cells. Tritium 196-198 neurotensin Mus musculus 199-210 3681301-4 1987 Clone N1E-115 degraded [3H]neurotensin in an apparently sequential fashion; the primary initial cleavage of intact neurotensin was at the peptide bond between residues Arg8 and Arg9. Tritium 24-26 neurotensin Mus musculus 27-38 3681301-7 1987 [3H]Neurotensin(8-13) was more rapidly hydrolyzed under identical conditions than was [3H]neurotensin itself. Tritium 1-3 neurotensin Mus musculus 4-15 3478712-4 1987 Within 2 min of stimulation of neutrophils with 10(-6) M PAF, the 7-fold increase in acetyltransferase activity was coincident with substantial PAF synthesis (as measured by [3H]acetate incorporation into PAF), which was 14% of the PAF synthesis induced by the Ca2+ ionophore A23187 at 10(-5) M. PAF activation of the acetyltransferase and PAF synthesis required intact neutrophils as they did not occur in cells broken by sonication. Tritium 175-177 acetyltransferase Escherichia coli 85-102 2820959-4 1987 HDL3 and TNM-HDL3 were found to be equally effective in causing efflux of plasma membrane cholesterol radiolabeled with [3H]cholesterol. Tritium 121-123 teneurin transmembrane protein 1 Homo sapiens 9-12 2820959-5 1987 However, HDL3 was much more effective than TNM-HDL3 in causing efflux of [3H]cholesterol associated with intracellular membranes. Tritium 74-76 teneurin transmembrane protein 1 Homo sapiens 43-46 2957134-9 1987 The net increase (over the baseline values) in [3H]thymidine incorporation in T-TA and CD8-TA by rIL-2 was significantly less in the aging group when compared to the young group. Tritium 48-50 CD8a molecule Homo sapiens 87-90 3598589-0 1987 [3H]GBR-12935 binding to the dopamine transporter is decreased in the caudate nucleus in Parkinson"s disease. Tritium 1-3 solute carrier family 6 member 3 Homo sapiens 29-49 3597435-13 1987 Since several lines of evidence indicate that [3H]Dex-Mes labeling of the receptor occurs in the steroid binding site, Cys-656 is the first amino acid which can be directly associated with a particular property of the glucocorticoid receptor. Tritium 47-49 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 218-241 3108322-2 1987 3H-Labeled PAF administered into the airway of isolated rat lungs was rapidly and extensively metabolized. Tritium 0-2 PCNA clamp associated factor Rattus norvegicus 11-14 3560164-1 1987 CCK-30-33 has been identified as the minimum fragment of CCK with nanomolar affinity for the central CCK receptors, as assayed by displacement of [3H]-Boc-beta-alanyl-CCK-30-33 (pentagastrin) in homogenized mouse cerebral cortex. Tritium 147-149 cholecystokinin Mus musculus 0-3 3560164-1 1987 CCK-30-33 has been identified as the minimum fragment of CCK with nanomolar affinity for the central CCK receptors, as assayed by displacement of [3H]-Boc-beta-alanyl-CCK-30-33 (pentagastrin) in homogenized mouse cerebral cortex. Tritium 147-149 cholecystokinin Mus musculus 57-60 3560164-1 1987 CCK-30-33 has been identified as the minimum fragment of CCK with nanomolar affinity for the central CCK receptors, as assayed by displacement of [3H]-Boc-beta-alanyl-CCK-30-33 (pentagastrin) in homogenized mouse cerebral cortex. Tritium 147-149 cholecystokinin Mus musculus 57-60 3560164-1 1987 CCK-30-33 has been identified as the minimum fragment of CCK with nanomolar affinity for the central CCK receptors, as assayed by displacement of [3H]-Boc-beta-alanyl-CCK-30-33 (pentagastrin) in homogenized mouse cerebral cortex. Tritium 147-149 cholecystokinin Mus musculus 57-60 3455050-1 1987 A number of progesterone derivatives, having a 17 alpha-acetoxy group and various functions at C-3 and C-6, interact at the cardiac glycoside (CG) binding site, using [3H]ouabain in a radioligand binding assay (RBA) with membranes from dog myocardium. Tritium 168-170 complement C6 Canis lupus familiaris 103-106 2439070-7 1987 Treatment of the DNA containing doubly-labelled AP sites with E. coli endonuclease III, then with E. coli endonuclease VI (exonuclease III), also results in the loss of 3H and the formation of a sugar phosphate with a lower 3H/32P ratio that behaves chromatographically as the beta-elimination product digested with E. coli endonuclease VI (exonuclease III). Tritium 169-171 endonuclease III Escherichia coli 70-86 2439070-7 1987 Treatment of the DNA containing doubly-labelled AP sites with E. coli endonuclease III, then with E. coli endonuclease VI (exonuclease III), also results in the loss of 3H and the formation of a sugar phosphate with a lower 3H/32P ratio that behaves chromatographically as the beta-elimination product digested with E. coli endonuclease VI (exonuclease III). Tritium 224-226 endonuclease III Escherichia coli 70-86 3030320-2 1987 Binding of [3H]-LTB4 was of high affinity (Kd = 0.76 nM), saturable and linear with protein concentration (0.2-1.2 mg/ml). Tritium 12-14 prostaglandin reductase 1 Cavia porcellus 16-20 3030320-4 1987 [3H]-LTB4 was unmetabolized during incubation with membrane preparations, as indicated by high performance liquid chromatography. Tritium 1-3 prostaglandin reductase 1 Cavia porcellus 5-9 3028272-1 1987 The in vitro stability of the Ah receptor from rat hepatic cytosol was evaluated by [3H]TCDD binding studies, gel filtration, and sucrose density gradient ultracentrifugation. Tritium 85-87 aryl hydrocarbon receptor Rattus norvegicus 30-41 3794718-2 1987 However, [3H]NECA, a nonselective A-1/A-2 agonist, gave reproducible binding, which was enhanced by removal of endogenous adenosine, using the catabolic enzyme adenosine deaminase. Tritium 10-12 UDP glucuronosyltransferase family 1 member A6 Rattus norvegicus 34-41 3794718-6 1987 The A-1-selective agonist CPA was a weak inhibitor of [3H]NECA binding (Ki = 251 nM). Tritium 55-57 UDP glucuronosyltransferase family 1 member A6 Rattus norvegicus 4-7 3033696-2 1987 The repeated stress induced a reduction in the maximum number of binding sites (Bmax) for (3H)dihydroalprenolol (DHA) in the cerebral cortex of rats with tap water, without affecting (3H)clonidine binding. Tritium 91-93 SEC14-like lipid binding 2 Rattus norvegicus 154-157 3790617-1 1987 Rat-liver glucocorticoid receptor was incubated with either [3H]triamcinolone acetonide or [3H]RU 486, a well known antiglucocorticoid. Tritium 61-63 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 10-33 2456672-3 1987 Addition of nonradiolabelled progesterone receptor from the chicken oviduct (PRov) successfully competes for the [3H]PRov binding to these acceptor sites in isolated chromatin or in nucleoacidic protein (NAP), a partially deproteinized chromatin enriched in these binding sites. Tritium 114-116 progesterone receptor Gallus gallus 29-50 3539226-2 1987 C71/26 immunoprecipitates as a broad band of mol wt 87,000 to 100,000 as determined by radioiodination of the platelet cell surface and by the 3H-sodium borohydride labeling technique. Tritium 143-145 ATPase, H+ transporting, lysosomal accessory protein 1 Mus musculus 0-3 3829120-6 1987 In situ hybridisation histochemistry using 3H cDNA revealed that CaBP mRNA visualised by silver grains was found in all the parts of the endometrium and the myometrium. Tritium 43-45 hippocalcin Rattus norvegicus 65-69 3583595-1 1987 The effect of castration and testosterone treatment on the distribution of [3H] radioactive and endogenous bound folates in hepatic cytosolic folate binding proteins (FBP-C) has been studied in rats. Tritium 76-78 far upstream element binding protein 1 Rattus norvegicus 167-170 3583595-2 1987 The distribution of [3H] radioactive bound folates in these FBP"s shows no significant difference in the three experimental group animals. Tritium 21-23 far upstream element binding protein 1 Rattus norvegicus 60-63 3819631-3 1987 The presence of [3H]mibolerone-androgen receptor complexes with a sedimentation coefficient of 7-8S was demonstrated in Harderian gland cytosol by a linear sucrose gradient ultracentrifugation technique using a vertical rotor. Tritium 17-19 androgen receptor Mesocricetus auratus 31-48 3025369-2 1987 [3H]Ro 15-4513 specifically and irreversibly labeled a protein with an apparent molecular weight of 51,000 (P51) in cerebellum and at least two proteins with apparent molecular weights of 51,000 (P51) and 55,000 (P55) in hippocampus. Tritium 1-3 MAGUK p55 scaffold protein 1 Rattus norvegicus 213-216 3794699-0 1987 Binding of [3H]Ro 16-6491, a reversible inhibitor of monoamine oxidase type B, to human brain mitochondria and platelet membranes. Tritium 12-14 monoamine oxidase B Homo sapiens 53-77 3794699-1 1987 The reversible inhibitor of monoamine oxidase type B (MAO-B) [3H]Ro 16-6491 binds specifically and with high affinity to a single population of binding sites in human frontal cortex crude mitochondria and platelet membranes. Tritium 62-64 monoamine oxidase B Homo sapiens 28-52 3794699-1 1987 The reversible inhibitor of monoamine oxidase type B (MAO-B) [3H]Ro 16-6491 binds specifically and with high affinity to a single population of binding sites in human frontal cortex crude mitochondria and platelet membranes. Tritium 62-64 monoamine oxidase B Homo sapiens 54-59 3614463-2 1987 3H labeled methotrexate (3H MTX) was covalently bound by means of aminopropyl carbodiimide to bovine and mouse fibrinogen (FBG). Tritium 0-2 metaxin 1 Mus musculus 28-31 3128785-6 1987 Trace [3H]-acetylated calmodulin, consisting predominantly of molecules acetylated at single sites together with unmodified protein, was incubated in excess (five- to 20-fold) with smooth muscle MLC kinase to allow the modified and unmodified molecules to compete for binding to the enzyme. Tritium 7-9 calmodulin 2 Gallus gallus 22-32 3536872-2 1986 During logarithmic growth these yeast cultures each contained about 40 microU (1 microU is 1 pmol of 3H released per min) of thymidylate synthase activity per 10(8) haploid cells, but as cultures entered the stationary phase and during the stationary phase, activity dropped dramatically, eventually reaching undetectable levels. Tritium 101-103 thymidylate synthase Saccharomyces cerevisiae S288C 125-145 3642713-0 1986 Degradation of solubilized [3H]elastin by intact human monocytes stimulated by immune complexes in vitro. Tritium 28-30 elastin Homo sapiens 31-38 3642713-2 1986 The substrate, solubilized [3H]elastin, is coated onto cell culture dishes. Tritium 28-30 elastin Homo sapiens 31-38 3464601-7 1986 The binding of [3H]PDBu to this fragment was dependent on calcium and phosphatidylserine and was of high affinity (Kd = 2.8 nM) and of essentially identical specificity to that of native protein kinase C. It is concluded that the 32-kDa fragment represents a lipid binding, regulatory domain of protein kinase C. Tritium 16-18 KIT proto-oncogene receptor tyrosine kinase Rattus norvegicus 187-201 3464601-7 1986 The binding of [3H]PDBu to this fragment was dependent on calcium and phosphatidylserine and was of high affinity (Kd = 2.8 nM) and of essentially identical specificity to that of native protein kinase C. It is concluded that the 32-kDa fragment represents a lipid binding, regulatory domain of protein kinase C. Tritium 16-18 KIT proto-oncogene receptor tyrosine kinase Rattus norvegicus 295-309 3533520-3 1986 In primary cultures of rat uterine cells (from 21-day-old rats), ER is degraded with a half-life of 3-4 h, as determined by pulse-chase experiments in which ER in cells are covalently labeled with [3H]TAZ (20 nM) and then exposed to a chase of 10(-6) M estradiol. Tritium 198-200 tafazzin, phospholipid-lysophospholipid transacylase Rattus norvegicus 201-204 3533521-1 1986 The intracellular location of progesterone receptor and tritiated progestin was assessed in chick oviduct before and after in vitro exposure to 5 nM [3H]Org 2058 ([6,7-3H]16 alpha-ethyl-21-hydroxy-19-nor-4-pregnene-3,20-dione) for 5 or 45 min at 4 or 37 C. The experiments were designed to allow the intracellular localization of occupied and unoccupied receptor in relatively intact tissue. Tritium 150-152 progesterone receptor Gallus gallus 30-51 3489777-2 1986 Resident peritoneal macrophages incubated with purified M-CSF for 48 hr inhibited lymphocyte proliferation to Con A, PHA, and listerial antigen as determined by [3H]TdR uptake, and inhibited Con A-stimulated lymphocyte IL 2 production. Tritium 162-164 colony stimulating factor 1 (macrophage) Mus musculus 56-61 3091696-8 1986 A [3H]-AII binding assay revealed high-affinity specific binding on spleen cells. Tritium 3-5 arginase type II Mus musculus 7-10 2427635-7 1986 The direct interaction of LPS and LBP was inferred from two types of evidence: first, immunoprecipitation of [3H]LPS from APRS by anti-LBP sera; and second, by the 125I-labeling of LBP when APRS-containing 125I-labeled 2-(p-azidosalicylamido)ethyl 1,3"-dithiopropionyl-LPS was photolysed. Tritium 110-112 lipopolysaccharide binding protein Homo sapiens 34-37 2427635-7 1986 The direct interaction of LPS and LBP was inferred from two types of evidence: first, immunoprecipitation of [3H]LPS from APRS by anti-LBP sera; and second, by the 125I-labeling of LBP when APRS-containing 125I-labeled 2-(p-azidosalicylamido)ethyl 1,3"-dithiopropionyl-LPS was photolysed. Tritium 110-112 lipopolysaccharide binding protein Homo sapiens 135-138 2427635-7 1986 The direct interaction of LPS and LBP was inferred from two types of evidence: first, immunoprecipitation of [3H]LPS from APRS by anti-LBP sera; and second, by the 125I-labeling of LBP when APRS-containing 125I-labeled 2-(p-azidosalicylamido)ethyl 1,3"-dithiopropionyl-LPS was photolysed. Tritium 110-112 lipopolysaccharide binding protein Homo sapiens 135-138 3768308-4 1986 Incubation of [3H]25-OH-D3 or [3H]25-OH-D3-ANG with rat DBP revealed that both compounds were specifically bound to a protein with a sedimentation coefficient of 4.1 S. Each was displaced with a 500-fold excess of 25-OH-D3. Tritium 15-17 D-box binding PAR bZIP transcription factor Rattus norvegicus 56-59 3768308-4 1986 Incubation of [3H]25-OH-D3 or [3H]25-OH-D3-ANG with rat DBP revealed that both compounds were specifically bound to a protein with a sedimentation coefficient of 4.1 S. Each was displaced with a 500-fold excess of 25-OH-D3. Tritium 31-33 angiogenin Rattus norvegicus 43-46 3768308-4 1986 Incubation of [3H]25-OH-D3 or [3H]25-OH-D3-ANG with rat DBP revealed that both compounds were specifically bound to a protein with a sedimentation coefficient of 4.1 S. Each was displaced with a 500-fold excess of 25-OH-D3. Tritium 31-33 D-box binding PAR bZIP transcription factor Rattus norvegicus 56-59 3768308-5 1986 When [3H]25-OH-D3-ANG was exposed to UV radiation in the presence of rat DBP followed by the addition of a 500-fold excess of 25-OH-D3, there was no displacement of tritium from the 4.1S peak. Tritium 6-8 angiogenin Rattus norvegicus 18-21 3768308-6 1986 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis autoradiographic analysis of [3H]25-OH-D3-ANG exposed to UV radiation in the presence of rat DBP followed by the addition of a 500-fold excess of 25-OH-D3 revealed one major band with a molecular weight of 52 000. Tritium 88-90 angiogenin Rattus norvegicus 100-103 3768308-7 1986 These data provide strong evidence that [3H]25-OH-D3-ANG was covalently linked to the rat DBP. Tritium 41-43 angiogenin Rattus norvegicus 53-56 3768308-7 1986 These data provide strong evidence that [3H]25-OH-D3-ANG was covalently linked to the rat DBP. Tritium 41-43 D-box binding PAR bZIP transcription factor Rattus norvegicus 90-93 2427130-5 1986 Scatchard analyses of 3H-IMI binding in the presence and absence of 0.5 microM aAGLP revealed that the inhibition of 3H-IMI binding was associated with an increased Kd, with no appreciable change in Bmax. Tritium 117-119 orosomucoid 1 Rattus norvegicus 79-84 2427130-6 1986 The inhibitory action of platelet-free plasma (PFP) on 3H-IMI binding to cerebral membranes was eliminated after treatment of PFP with specific antibodies to aAGLP. Tritium 55-57 orosomucoid 1 Rattus norvegicus 158-163 3733875-1 1986 Histone H1 was purified from bovine thymus and radiolabeled with tritium by reductive methylation or with 125I using chloramine-T. Tritium 65-72 H1.0 linker histone Homo sapiens 0-10 2945992-4 1986 5-HT1A, 5-HT1B and 5-HT1C recognition sites were labelled with [3H]8-OH-DPAT([3H]8-hydroxy-2-(di-n-propylamino)-tetralin) in pig cortex membranes, [125I]CYP([125I]iodocyanopindolol) in rat cortex and [3H]mesulergine in pig choroid plexus membranes, respectively. Tritium 64-66 5-hydroxytryptamine receptor 2C Rattus norvegicus 19-25 2945992-4 1986 5-HT1A, 5-HT1B and 5-HT1C recognition sites were labelled with [3H]8-OH-DPAT([3H]8-hydroxy-2-(di-n-propylamino)-tetralin) in pig cortex membranes, [125I]CYP([125I]iodocyanopindolol) in rat cortex and [3H]mesulergine in pig choroid plexus membranes, respectively. Tritium 78-80 5-hydroxytryptamine receptor 2C Rattus norvegicus 19-25 3788408-4 1986 The changed 14C/3H ratio in AGP of diabetic rats suggested disturbed glycosylation of AGP in this disease. Tritium 16-18 orosomucoid 1 Rattus norvegicus 28-31 3788408-4 1986 The changed 14C/3H ratio in AGP of diabetic rats suggested disturbed glycosylation of AGP in this disease. Tritium 16-18 orosomucoid 1 Rattus norvegicus 86-89 3099750-7 1986 The 100,000 Mr secreted C1 Inh is sensitive to endoglycosidase F but resistant to endoglycosidase H, and it incorporates [3H]galactose, [3H]glucosamine and [3H]galactosamine, indicating the presence of both N-linked oligosaccharides of the complex type and O-linked oligosaccharides. Tritium 122-124 serpin family G member 1 Homo sapiens 24-30 3099750-7 1986 The 100,000 Mr secreted C1 Inh is sensitive to endoglycosidase F but resistant to endoglycosidase H, and it incorporates [3H]galactose, [3H]glucosamine and [3H]galactosamine, indicating the presence of both N-linked oligosaccharides of the complex type and O-linked oligosaccharides. Tritium 137-139 serpin family G member 1 Homo sapiens 24-30 3099750-7 1986 The 100,000 Mr secreted C1 Inh is sensitive to endoglycosidase F but resistant to endoglycosidase H, and it incorporates [3H]galactose, [3H]glucosamine and [3H]galactosamine, indicating the presence of both N-linked oligosaccharides of the complex type and O-linked oligosaccharides. Tritium 137-139 serpin family G member 1 Homo sapiens 24-30 3754874-6 1986 Purpurin binds [3H]retinol, and both purpurin and chick serum RBP stimulate the adhesion of neural retina cells, although the serum protein is less active than purpurin. Tritium 16-18 retinol binding protein 4 Gallus gallus 0-8 2870921-3 1986 When [methyl-3H]methylmalonyl-CoA was incubated with highly purified methylmalonyl-CoA mutase, tritium exchange with the medium depended on added methylmalonyl-CoA epimerase. Tritium 13-15 methylmalonyl-CoA mutase Homo sapiens 69-93 2870921-3 1986 When [methyl-3H]methylmalonyl-CoA was incubated with highly purified methylmalonyl-CoA mutase, tritium exchange with the medium depended on added methylmalonyl-CoA epimerase. Tritium 95-102 methylmalonyl-CoA mutase Homo sapiens 69-93 2870921-4 1986 With highly purified preparations of methylmalonyl-CoA mutase, effective tritium exchange from [5"-3H]adenosylcobalamin to water required the addition of methylmalonyl-CoA epimerase and of substrate (e.g. succinyl-CoA). Tritium 73-80 methylmalonyl-CoA mutase Homo sapiens 37-61 2870121-10 1986 Both dAdo and 2-chlorodeoxyadenosine blocked unscheduled DNA synthesis in irradiated resting lymphocytes, as measured by [3H]thymidine uptake. Tritium 122-124 ado Drosophila melanogaster 5-9 3005503-3 1986 Both probes bound with high affinity to the mouse cerebral cortical CCK receptor binding site with apparent equilibrium dissociation constants (KD) of 1.9 nM and 1.4 nM for [3H]pentagastrin and [125I]BH CCK 8, respectively. Tritium 174-176 cholecystokinin Mus musculus 68-71 3963372-3 1986 The method is applicable for 14C- and 3H-labeled samples on TLC sheets (10 X 10 cm) emitting more than 0.17 and 7.5 Bq/mm2, respectively. Tritium 38-40 PNMA family member 2 Homo sapiens 119-122 3010168-1 1986 The ability of N,N-diallyl-Tyr-Aib-Aib-Phe-Leu-OH (ICI 174864) to displace [3H]-[D-Ala2, D-Leu5]enkephalin bound to the delta-opioid site is increased 8-16 fold by addition of 25mM NaCl. Tritium 76-78 ANIB1 Homo sapiens 31-34 3010168-1 1986 The ability of N,N-diallyl-Tyr-Aib-Aib-Phe-Leu-OH (ICI 174864) to displace [3H]-[D-Ala2, D-Leu5]enkephalin bound to the delta-opioid site is increased 8-16 fold by addition of 25mM NaCl. Tritium 76-78 ANIB1 Homo sapiens 35-38 3004471-2 1986 The relative order of potencies for gastrin and CCK analogs in displacing [3H]pentagastrin binding correlated well with those obtained using [125I]gastrin and their reported biological potencies for stimulating acid secretion. Tritium 75-77 cholecystokinin Cavia porcellus 48-51 3004471-3 1986 Nonselective CCK/gastrin antagonists including carbobenzoxy-CCK (26-32), proglumide and benzotript, but not the selective peripheral CCK antagonist, asperlicin, inhibited specific [3H]pentagastrin binding. Tritium 181-183 cholecystokinin Cavia porcellus 13-16 3467897-2 1986 Chromosomal assignment of the gene coding for human TdT was accomplished by in situ hybridization of a 3H-labeled cDNA probe to human chromosome preparations and by Southern blot analysis of somatic cell hybrid DNAs. Tritium 103-105 DNA nucleotidylexotransferase Homo sapiens 52-55 2420952-1 1986 A recessive temperature-sensitive paralytic mutation, tip-E, is associated with reduced binding of [3H]saxitoxin to voltage-sensitive sodium channels in membranes from adult Drosophila heads. Tritium 100-102 temperature-induced paralytic E Drosophila melanogaster 54-59 3084804-5 1986 In contrast, 3H-(GABA) was scarcely accumulated by glial-fibrillary-acidic-protein (GFAP)-positive astrocytes, but taken up by glutamate-decarboxylase-positive inhibitory interneurons and was released in a Ca2+-dependent way upon depolarization: 3H-GABA evoked release progressively increased with time in culture. Tritium 13-15 glial fibrillary acidic protein Rattus norvegicus 51-82 3084804-5 1986 In contrast, 3H-(GABA) was scarcely accumulated by glial-fibrillary-acidic-protein (GFAP)-positive astrocytes, but taken up by glutamate-decarboxylase-positive inhibitory interneurons and was released in a Ca2+-dependent way upon depolarization: 3H-GABA evoked release progressively increased with time in culture. Tritium 13-15 glial fibrillary acidic protein Rattus norvegicus 84-88 3084804-5 1986 In contrast, 3H-(GABA) was scarcely accumulated by glial-fibrillary-acidic-protein (GFAP)-positive astrocytes, but taken up by glutamate-decarboxylase-positive inhibitory interneurons and was released in a Ca2+-dependent way upon depolarization: 3H-GABA evoked release progressively increased with time in culture. Tritium 13-15 glutamate-ammonia ligase Rattus norvegicus 127-150 2419797-4 1985 On embryonic day (E) 14, [3H]thymidine injection labelled 12.8% of GAD-positive (+) neurons in sections of the area dentata processed at an age of 40 days postnatally. Tritium 26-28 glutamate-ammonia ligase Rattus norvegicus 67-70 4092742-0 1985 Binding of [3H]-4,5-secodihydrotestosterone by androgen receptor. Tritium 12-14 androgen receptor Rattus norvegicus 47-64 4062288-1 1985 The acylation of salivary mucin with fatty acids and its biosynthesis was investigated by incubating rat submandibular salivary gland cells with [3H]palmitic acid and [3H]proline. Tritium 146-148 solute carrier family 13 member 2 Rattus norvegicus 26-31 4062288-1 1985 The acylation of salivary mucin with fatty acids and its biosynthesis was investigated by incubating rat submandibular salivary gland cells with [3H]palmitic acid and [3H]proline. Tritium 168-170 solute carrier family 13 member 2 Rattus norvegicus 26-31 4042983-8 1985 [3H]hCG alpha, but not 3H-labeled free alpha, was able to combine with native hCG beta-subunit. Tritium 1-3 chorionic gonadotropin subunit beta 3 Homo sapiens 78-86 3840173-9 1985 The functional capacity of vitamin D-binding protein to bind hormone, assessed by the internalization of [3H]1,25-(OH)2D3 by intestinal epithelial cells in the presence of serum was not significantly different from normal (11.42 +/- 1.45 vs. 10.27 +/- 1.27 fmol/2 X 10(6) cells, respectively). Tritium 106-108 GC vitamin D binding protein Homo sapiens 27-52 2411531-7 1985 A comparison of the peptides produced after limited digestion with trypsin of 125I-labeled CaBP and [3H]tyrosine-labeled translation product indicated a good coincidence of peaks from purified 125I-labeled CaBP and the immunoprecipitated translation product, suggesting that the immunoprecipitated translation product is indeed vitamin D-dependent renal CaBP. Tritium 101-103 hippocalcin Rattus norvegicus 206-210 2411531-7 1985 A comparison of the peptides produced after limited digestion with trypsin of 125I-labeled CaBP and [3H]tyrosine-labeled translation product indicated a good coincidence of peaks from purified 125I-labeled CaBP and the immunoprecipitated translation product, suggesting that the immunoprecipitated translation product is indeed vitamin D-dependent renal CaBP. Tritium 101-103 hippocalcin Rattus norvegicus 206-210 3161947-7 1985 The inhibitory effects of DEX on the antigen-induced release of 5-lipoxygenase pathway products were confirmed by the physical measurements of 5-hydroxy-eicosatetraenoic acid (5-HETE) and LTC4, and by isotopic measurement of [3H]LTC4 after reverse-phase high performance liquid chromatography (RP-HPLC). Tritium 226-228 arachidonate 5-lipoxygenase Mus musculus 64-78 4068702-3 1985 The validity of the present exchange assay is based on the following: a similar quantity of androgen receptor was detected when binding was measured directly after in vivo treatment with radioactive androgen or indirectly by [3H]DHT exchange after treatment with non-radioactive androgen. Tritium 226-228 androgen receptor Rattus norvegicus 92-109 2995978-7 1985 The proportion of total specific binding corresponding to mu1 sites varied among the regions, ranging from 14% to 75% for [3H][D-Ala2, D-Leu5]enkephalin and 20% to 52% for [3H]dihydromorphine. Tritium 123-125 sodium voltage-gated channel alpha subunit 4 Rattus norvegicus 58-61 4030768-5 1985 [3H]Palmitate labeled a series of splenic cell proteins, including gp2, gp3, and gp3pr. Tritium 1-3 glycoprotein 2 (zymogen granule membrane) Mus musculus 67-70 4030768-7 1985 Splenic cells incorporated [3H]galactose in both gp2 and gp3 but not in gp3pr. Tritium 28-30 glycoprotein 2 (zymogen granule membrane) Mus musculus 49-52 4030955-5 1985 Interaction of [3H]Arg8-vasopressin ([3H]AVP) with NP-II was examined by chromatography of AVP on both CPG and NPG affinity matrices. Tritium 16-18 arginine vasopressin Bos taurus 51-56 2994869-0 1985 Ca2+ channel antagonist actions in bladder smooth muscle: comparative pharmacologic and [3H]nitrendipine binding studies. Tritium 89-91 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 0-3 3883999-3 1985 The effect of IGF II on [3H] thymidine incorporation in cell cultures of fetal bovine liver was similar to the effect of ET. Tritium 25-27 insulin like growth factor 2 Bos taurus 14-20 3871405-2 1985 The production of CSF-2 was enhanced by endotoxin and inhibited by serum, and correlated inversely with [3H]TdR incorporation. Tritium 105-107 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 18-23 4036450-1 1985 Methionine synthase reaction may be coupled with thymidylate synthase-catalysed tritium release from [5-3H]dUMP via non-enzymatic reaction of formaldehyde with tetrahydrofolate. Tritium 80-87 5-methyltetrahydrofolate-homocysteine methyltransferase Homo sapiens 0-19 2858199-1 1985 The regulation of synthesis of the gluconeogenic cytosolic enzyme phosphoenolpyruvate carboxykinase (PEPCK) and of tyrosine aminotransferase (TAT) by glucagon and glucocorticoid hormones was studied in hepatocytes maintained in suspension culture for 7 h. Specific antibodies were used to measure relative rates of enzyme synthesis after pulse-labelling of the cells with [3H]leucine or [35S]methionine. Tritium 373-375 phosphoenolpyruvate carboxykinase 1 Rattus norvegicus 101-106 3836703-3 1985 2.1.1.8; HMT) in the presence of the natural donor of methyl groups, [3H]-methyl-S-adenosyl-L-methionine ([3H]-SAMe). Tritium 70-72 histamine N-methyltransferase Homo sapiens 9-12 3836703-3 1985 2.1.1.8; HMT) in the presence of the natural donor of methyl groups, [3H]-methyl-S-adenosyl-L-methionine ([3H]-SAMe). Tritium 107-109 histamine N-methyltransferase Homo sapiens 9-12 4053573-2 1985 Column chromatographic separation of the neutral [3H]- and [14C]dGlc from the [3H]- and [14C]2-deoxy-D-glucose-6-phosphate permitted estimation of the quantity of [3H]dGlc phosphorylated in 2 min, and the proportion of [14C]dGlc phosphorylated in 1 min; thus a phosphorylation rate was determined from a single tissue sample. Tritium 50-52 beta glucuronidase Drosophila melanogaster 167-171 4053573-2 1985 Column chromatographic separation of the neutral [3H]- and [14C]dGlc from the [3H]- and [14C]2-deoxy-D-glucose-6-phosphate permitted estimation of the quantity of [3H]dGlc phosphorylated in 2 min, and the proportion of [14C]dGlc phosphorylated in 1 min; thus a phosphorylation rate was determined from a single tissue sample. Tritium 50-52 beta glucuronidase Drosophila melanogaster 167-171 4053573-2 1985 Column chromatographic separation of the neutral [3H]- and [14C]dGlc from the [3H]- and [14C]2-deoxy-D-glucose-6-phosphate permitted estimation of the quantity of [3H]dGlc phosphorylated in 2 min, and the proportion of [14C]dGlc phosphorylated in 1 min; thus a phosphorylation rate was determined from a single tissue sample. Tritium 79-81 beta glucuronidase Drosophila melanogaster 64-68 4053573-2 1985 Column chromatographic separation of the neutral [3H]- and [14C]dGlc from the [3H]- and [14C]2-deoxy-D-glucose-6-phosphate permitted estimation of the quantity of [3H]dGlc phosphorylated in 2 min, and the proportion of [14C]dGlc phosphorylated in 1 min; thus a phosphorylation rate was determined from a single tissue sample. Tritium 79-81 beta glucuronidase Drosophila melanogaster 64-68 2995401-6 1985 Flash-labeled autoradiographs revealed that most 3H-thymidine incorporating cancer cells were located around the middle layer, with a small amount of mucin. Tritium 49-51 mucin Canis lupus familiaris 150-155 6543324-4 1984 In our study, a 50% displacement of radiolabeled 25-hydroxyvitamin D3 ([3H]25OHD3) from its initial binding is achieved with 325 fmol/mL 25(R),26(OH)2D3 and 850-1000 fmol/mL 25(S),26(OH)2D3--a difference in potency of approximately 3-fold. Tritium 72-74 adaptor-related protein complex 2, alpha 2 subunit Mus musculus 134-139 6543324-4 1984 In our study, a 50% displacement of radiolabeled 25-hydroxyvitamin D3 ([3H]25OHD3) from its initial binding is achieved with 325 fmol/mL 25(R),26(OH)2D3 and 850-1000 fmol/mL 25(S),26(OH)2D3--a difference in potency of approximately 3-fold. Tritium 72-74 adaptor-related protein complex 2, alpha 2 subunit Mus musculus 171-176 6527796-3 1984 [3H]Acetylcholine synthesis and accumulation by 2-3-week old nodose cultures and choline acetyltransferase activity were increased (2.0 +/- 0.15)-fold and (2.0 +/- 0.48)-fold, respectively, upon growth with muscle-conditioned medium, whereas acetylcholinesterase was decreased (1.5 +/- 0.1)-fold (means +/- SEM, n = 5-9). Tritium 1-3 choline O-acetyltransferase Rattus norvegicus 81-106 6092383-1 1984 Thyroxine-binding globulin (TBG) synthesis by human hepatoma (Hep G2) cells was demonstrated by pulse labeling with [35S]methionine or [3H]mannose and subsequent immunoprecipitation in the medium or cell lysate. Tritium 136-138 serpin family A member 7 Homo sapiens 0-26 6092383-1 1984 Thyroxine-binding globulin (TBG) synthesis by human hepatoma (Hep G2) cells was demonstrated by pulse labeling with [35S]methionine or [3H]mannose and subsequent immunoprecipitation in the medium or cell lysate. Tritium 136-138 serpin family A member 7 Homo sapiens 28-31 6542056-0 1984 Penetration of [3H]T-2 toxin through excised human and guinea-pig skin during exposure to [3H]T-2 toxin adsorbed to corn dust. Tritium 16-18 solute carrier family 25 member 5 Homo sapiens 19-22 6436057-1 1984 Platelet-activating factor(PAF) induced the rapid hydrolysis of phospholipids in Swiss mouse 3T3 fibroblasts prelabeled with [3H]arachidonic acid or [3H]choline. Tritium 126-128 patchy fur Mus musculus 0-31 6086276-5 1984 Its ability to compete with [3H]dexamethasone for binding to the renal glucocorticoid receptor was only 1.9% in comparison to unlabeled dexamethasone. Tritium 29-31 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 71-94 6149522-2 1984 Compared to control, [3H]DA uptake increased significantly when the synaptosomal fraction of CP was incubated with 0.1 and 1 microM of alpha-MSH and also when the rat was previously injected with alpha-MSH. Tritium 22-24 proopiomelanocortin Rattus norvegicus 135-144 6149522-2 1984 Compared to control, [3H]DA uptake increased significantly when the synaptosomal fraction of CP was incubated with 0.1 and 1 microM of alpha-MSH and also when the rat was previously injected with alpha-MSH. Tritium 22-24 proopiomelanocortin Rattus norvegicus 196-205 6427217-13 1984 When isolated bovine neural retinas were incubated with 3H-labeled fucose, glucosamine, or leucine, a solitary labeled protein identified as IRBP was secreted into the medium. Tritium 56-58 retinol binding protein 3 Bos taurus 141-145 6427217-15 1984 Neural retinas incubated with 3H-labeled leucine in the presence of tunicamycin secreted a form of IRBP that did not bind concanavalin A and had an Mr reduced by approximately 5,000. Tritium 30-32 retinol binding protein 3 Bos taurus 99-103 6370311-1 1984 Five different vascular beds of the cat were tested for their ability to metabolize tritium-labeled or unlabeled prostacyclin by 15-hydroxyprostaglandin dehydrogenase in vivo. Tritium 84-91 carbonyl reductase 1 Homo sapiens 129-166 6609069-2 1984 [3H]DHT and [3H]R1881 had similar affinities for the androgen receptor in vitro (Kd = 0.3 nM). Tritium 1-3 androgen receptor Rattus norvegicus 53-70 6609069-2 1984 [3H]DHT and [3H]R1881 had similar affinities for the androgen receptor in vitro (Kd = 0.3 nM). Tritium 13-15 androgen receptor Rattus norvegicus 53-70 6723626-2 1984 Comparative pulse-chase experiments with [3H]palmitic acid and [35S]methionine revealed that a precursor polypeptide, designated p62, of the structural SFV glycoprotein and E1 serve as the primary acceptors of acyl chains. Tritium 42-44 nucleoporin 62 Homo sapiens 129-132 6325601-11 1984 The distribution of CCK binding sites labeled with [3H]CCK8 appears to correlate better with the CCK immunoreactivity than those labeled with [125I]Bolton-Hunter-CCK33. Tritium 52-54 cholecystokinin Rattus norvegicus 20-23 6325601-11 1984 The distribution of CCK binding sites labeled with [3H]CCK8 appears to correlate better with the CCK immunoreactivity than those labeled with [125I]Bolton-Hunter-CCK33. Tritium 52-54 cholecystokinin Rattus norvegicus 55-58 6325601-12 1984 Thus, [3H]CCK8 appears to be the radioligand of choice for the investigation of CCK receptors. Tritium 7-9 cholecystokinin Rattus norvegicus 10-13 6327965-3 1984 There is a linear correlation between the ability to compete with (-)-[3H]I for the m-AChR and the affinity constant of the analogue as determined by in vitro assay, suggesting that the test is a valid indicator of in vivo distribution. Tritium 71-73 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 86-90 6561956-0 1984 Adsorbed soluble [3H]elastin as substrate for proteinase activity. Tritium 18-20 elastin Homo sapiens 21-28 6328368-1 1984 The dipeptide, L-phenylalanyl-L-glutamate (PG), augments the specific binding of the excitatory amino acid receptor antagonist, [3H]2-amino-7-phosphonoheptanoic acid (APH), to rat forebrain membranes by 5-fold at 100 microM with an EC50 of 4.9 microM. Tritium 129-131 acylaminoacyl-peptide hydrolase Rattus norvegicus 167-170 6368533-5 1984 The decay curves of [3H]leucine-labeled SDH showed that these hormones did not affect the rate of enzyme degradation. Tritium 21-23 serine dehydratase Rattus norvegicus 40-43 6139373-8 1983 Subsequent fragmentation of [3H]C3b with porcine elastase further localized the label to the C3d subdomain. Tritium 29-31 complement C3 Homo sapiens 32-35 6139373-10 1983 A tryptic peptide containing the 3H label was isolated following digestion of [3H]C3b on activated thiol-Sepharose. Tritium 33-35 complement C3 Homo sapiens 82-85 6139373-10 1983 A tryptic peptide containing the 3H label was isolated following digestion of [3H]C3b on activated thiol-Sepharose. Tritium 79-81 complement C3 Homo sapiens 82-85 6667712-0 1983 Decrease in [3H]cocaine binding to the dopamine transporter in Parkinson"s disease. Tritium 13-15 solute carrier family 6 member 3 Homo sapiens 39-59 6605386-2 1983 Responsiveness to macrophage type L cell CSF was measured by two different assays: by determination of [3H]thymidine (3H-TdR) incorporation and by enumeration of colonies formed in response to CSF. Tritium 104-106 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 41-44 6414473-1 1983 Human Tamm-Horsfall urinary glycoprotein from an individual of the blood group Sd(a+) phenotype was tritium-labelled by treatment with galactose oxidase and sodium boro[3H]hydride and was then digested with endo-beta-galactosidase. Tritium 100-107 uromodulin Homo sapiens 6-40 6414473-1 1983 Human Tamm-Horsfall urinary glycoprotein from an individual of the blood group Sd(a+) phenotype was tritium-labelled by treatment with galactose oxidase and sodium boro[3H]hydride and was then digested with endo-beta-galactosidase. Tritium 169-171 uromodulin Homo sapiens 6-40 6832566-2 1983 The labeling index of gastrin cells after five daily injections of [3H]thymidine given during bombesin treatment was significantly enhanced as compared with controls, while no modification in the pattern of antral somatostatin cell labeling was revealed. Tritium 68-70 gastrin Rattus norvegicus 22-29 6605848-4 1983 Inhibition experiments revealed that the binding to 3H-R 1881 in cytosols of these tumors was attributable to the androgen receptor. Tritium 52-54 androgen receptor Mus musculus 114-131 6601026-5 1983 The steroid also suppressed CSF-independent [3H]thymidine uptake, but the time course of suppression obviously differed from that of CSF-dependent uptake. Tritium 45-47 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 28-31 6600767-0 1983 Stimulation of [3H]thymidine uptake in mouse marrow by granulocyte-macrophage colony stimulating factor from mouse lung conditioned medium. Tritium 16-18 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 55-103 6600767-1 1983 Stimulation of [3H]thymidine uptake in mouse marrow cells by a haematopoietic factor, granulocyte-macrophage colony stimulating factor from mouse lung conditioned medium, was used to follow the activity of the factor in the medium, and during its partial purification from the medium. Tritium 16-18 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 86-134 6958909-7 1982 These macrophages demonstrated 90% inhibition of [3H]thymidine incorporation by EL 4 tumor cells at a 100:1 effector-to-target cell ratio. Tritium 50-52 epilepsy 4 Mus musculus 80-84 7155461-0 1982 Regional specificity of changes in [3H]Leu-enkephalin binding associated with warm water swimming in mice. Tritium 36-38 prodynorphin Mus musculus 39-53 7047684-5 1982 In vivo, [3H]thymidine retention in CSF and brain after entry from blood was increased when the efflux of [3H]thymidine from CSF and the phosphorylation of [3H]thymidine in brain were depressed by the intraventricular injection of unlabeled thymidine. Tritium 10-12 colony stimulating factor 2 Rattus norvegicus 36-39 7047684-5 1982 In vivo, [3H]thymidine retention in CSF and brain after entry from blood was increased when the efflux of [3H]thymidine from CSF and the phosphorylation of [3H]thymidine in brain were depressed by the intraventricular injection of unlabeled thymidine. Tritium 10-12 colony stimulating factor 2 Rattus norvegicus 125-128 7047684-5 1982 In vivo, [3H]thymidine retention in CSF and brain after entry from blood was increased when the efflux of [3H]thymidine from CSF and the phosphorylation of [3H]thymidine in brain were depressed by the intraventricular injection of unlabeled thymidine. Tritium 107-109 colony stimulating factor 2 Rattus norvegicus 36-39 7047684-5 1982 In vivo, [3H]thymidine retention in CSF and brain after entry from blood was increased when the efflux of [3H]thymidine from CSF and the phosphorylation of [3H]thymidine in brain were depressed by the intraventricular injection of unlabeled thymidine. Tritium 107-109 colony stimulating factor 2 Rattus norvegicus 125-128 6294632-1 1982 [3H]Neurotensin ([3H]NT) appears to bind specifically to a single class of sites in slide-mounted rat kidney sections (KD = 8.3 nM; Bmax = 31.6 fmol/mg tissue). Tritium 1-3 neurotensin Rattus norvegicus 4-15 6294632-1 1982 [3H]Neurotensin ([3H]NT) appears to bind specifically to a single class of sites in slide-mounted rat kidney sections (KD = 8.3 nM; Bmax = 31.6 fmol/mg tissue). Tritium 18-20 neurotensin Rattus norvegicus 4-15 6294639-2 1982 Displacements of both 3H-ligands by metkephamid indicate that metkephamid interacts competitively with greatest potency to the high affinity binding component for both ligands (mu1 site). Tritium 22-24 sodium voltage-gated channel alpha subunit 4 Rattus norvegicus 177-180 6287338-3 1982 A micro-dissection of coronal sections of guinea-pig hippocampus revealed that the specific binding capacity for [3H]CHA in area CA-1 was 20-30% higher than in area CA-3, dentate gyrus and subiculum. Tritium 114-116 carbonic anhydrase 3 Cavia porcellus 165-169 7200801-4 1982 Subsequent addition of retinol to the retinoid-RBP complex resulted in complete displacement of IBA and RBA from the protein, whereas a large proportion (37%) of the [3H]IEBA remained bound to the retinol binding site of RBP. Tritium 167-169 retinol binding protein 4 Homo sapiens 47-50 7200801-6 1982 The amount of IEBA remaining bound to RBP increased with increasing incubation time, reaching a maximum of about 0.66 mol/mol of RBP at 18 h. Moreover, at each time point, the binding of retinol to RBP was inhibited to an extent that was equivalent to the amount of [3H]IEBA that was not displaced from RBP by retinol. Tritium 267-269 retinol binding protein 4 Homo sapiens 38-41 6280803-8 1982 [3H]CHA binding is highly dependent in the presence of adenosine deaminase since only 30% of the binding capacity is observed in synaptosomal membranes not treated with this enzyme. Tritium 1-3 adenosine deaminase Rattus norvegicus 55-74 7326232-2 1981 By all criteria, [3H]-alpha-Bgt and 125I-labeled monoiodinated alpha-Bgt ([125I]-alpha-Bgt) exhibited comparable specificities and affinities for nAChR. Tritium 18-20 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 146-151 7309918-3 1981 The number of cells taking up 3H-Tdr label per mm2, as detected by autoradiography, was similar in 14-day-old GVHD and control tissue as was the area of the germinal matrix zone and the number of mitotically active germinal cells per mm2 in sagittal sections near the midline. Tritium 30-32 PNMA family member 2 Homo sapiens 47-50 7030400-2 1981 Monocyte cell lysates exhibited activity at neutral pH against azocasein, 3H-labelled elastin as well as several synthetic substrates used to detect serine proteinases (EC 3.4.21.-) of human polymorphonuclear leucocytes. Tritium 74-76 elastin Homo sapiens 86-93 6975208-1 1981 Androgen receptor measurements with [3H]-methyltrienolone [17 beta-hydroxy-17 alpha-methyl-estra-4,9,11-trien-3-one (R1881)] or [3H]testosterone in vastus lateralis muscle cytosol of intact male rats yielded similar numbers of binding sites. Tritium 37-39 androgen receptor Rattus norvegicus 0-17 7028466-4 1981 In the absence of vasopressin blockade, blood pressure and heart rate did not change significantly, while plasma renin activity increased from 3.8 +/- 0.9 to 10.8 +/- 3.1 ng/ml/3h (P less than 0.005), and plasma corticosteroid concentration increased from 1.5 +/- 0.8 to 8.6 +/- 2.0 microgram/dl (P less than 0.001). Tritium 177-179 renin Canis lupus familiaris 113-118 6791509-1 1981 The effects of cholecystokinin (CCK) on [3H]leucine incorporation into protein were studied in isolated pancreatic acini from normal and streptozotocin-induced diabetic rats. Tritium 41-43 cholecystokinin Rattus norvegicus 32-35 6791509-2 1981 CCK8, the biologically active C-terminal octapeptide of CCK, increased [3H]leucine incorporation into protein over a concentration range that stimulates digestive enzyme secretion (1 X 10(-11) to 1 X 10(-10) M); at higher concentrations, CCK8 inhibited incorporation. Tritium 72-74 cholecystokinin Rattus norvegicus 0-3 6169709-2 1981 Cytochrome P-450 mRNA activity was detected near 18S in the sucrose density gradient, accounting for approximately 5% of total mRNA activity on the basis of [3H]leucine incorporation in an in vitro translation system of wheat germ. Tritium 158-160 cytochrome P450 709B1 Triticum aestivum 0-16 6115565-2 1981 3H-Thymidine incorporation into lymphocytes was completely inhibited by F(ab1)2 fragments of normal human IgG containing no antibody to the viruses examined. Tritium 0-2 phosphoinositide kinase, FYVE-type zinc finger containing Homo sapiens 72-79 7288405-5 1981 GP1 was an atypical glycopolypeptide in that 35S-methionine incorporation was poor relative to 3H-glucosamine incorporation. Tritium 95-97 GTP binding protein 1 Homo sapiens 0-3 6451618-3 1981 Incubation of [3H]N-ethylmaleimide-SF1(A1) with N-ethylmaleimide-SF1(A2) in the presence of 10 mM MgATP at 37 degrees C resulted in the exchange of subunits between these isozymes. Tritium 15-17 splicing factor 1 Homo sapiens 35-38 6451618-3 1981 Incubation of [3H]N-ethylmaleimide-SF1(A1) with N-ethylmaleimide-SF1(A2) in the presence of 10 mM MgATP at 37 degrees C resulted in the exchange of subunits between these isozymes. Tritium 15-17 splicing factor 1 Homo sapiens 65-68 6161887-3 1981 Fragments obtained by dialysis of bovine serum albumin (BSA) labelled with tritium gas by Wilzbach method, bound to normal rabbit serum in vitro and in vivo. Tritium 75-82 albumin Rattus norvegicus 41-54 6260763-9 1981 The pKa for His-91 was shifted to the alkaline side in the presence of 3"-GMP, a competitive inhibitor, in the titration plots observed by both hydrogen-tritium exchange and 1H NMR spectroscopy. Tritium 153-160 5'-nucleotidase, cytosolic II Homo sapiens 74-77 6110749-4 1980 However, binding studies with [3H]pargyline, which provided an estimate of the respective concentrations of MAO-A and MAO-B active centres in heart mitochondria, indicated a ratio between MAO-A and MAO-B, markedly different from that shown by plots of inhibition of benzylamine metabolism by various concentrations of clorgyline. Tritium 31-33 monoamine oxidase B Rattus norvegicus 198-203 7429701-6 1980 The control and test electrodes each contained 1.0 ml tritium-labeled Dm-Na-P (approximately 4.0 mg) and 2.0 ml 4% lidocaine HCl (80 mg). Tritium 54-61 catenin beta like 1 Homo sapiens 73-77 6780919-2 1980 The release of [3H]GABA increased 114% and 136% over control values in P1 and P2 fractions, respectively. Tritium 16-18 perforin 1 Gallus gallus 71-80 6780919-7 1980 A23187 enhanced [3H]GABA release from P1 and P2 by 52% and 105%, respectively. Tritium 17-19 perforin 1 Gallus gallus 38-47 6966605-3 1980 CPN treatment greatly increased [3H]-thymidine incorporation by human lymphocytes at lower concentrations of PWM. Tritium 33-35 carboxypeptidase N subunit 1 Homo sapiens 0-3 6966605-5 1980 When CPN-treated non-T cells were added to autologous T cells, [3H]-thymidine incorporation was markedly enhanced at lower concentrations of PWM. Tritium 64-66 carboxypeptidase N subunit 1 Homo sapiens 5-8 6775326-1 1980 Following previous histological studies on the problem of so-called psychopharmacotoxic encephalopathy, we have mesured the incorporation of 3-H-4.5 leucine into the limbic systm (Ncl. Tritium 141-144 nucleolin Mus musculus 180-183 575766-6 1979 The straight line from the corrected points gives the specific binding parameters: number of specific binding sites: about 0,5 moles 3H-NQNO/ moles cytochrome b and KD= 50 nM. Tritium 133-135 mitochondrially encoded cytochrome b Homo sapiens 148-160 93580-2 1979 From the nucliec acid hybridization studies, the maximum levels of hybridization were obtained for both N- and X-tropic BALB/c endogenous retrovirus specific [3H]-cDNAs with liver RNA from animals receiving BrdUrd at 40 and 44 h post-CCl4 treatment, and killed on the fourth day following BrdUrd injection. Tritium 159-161 chemokine (C-C motif) ligand 4 Mus musculus 234-238 477596-1 1979 A technique is described for labeling bovine parathyroid hormone (bPTH) with tritium by [3Hmethyl exchange. Tritium 77-84 parathyroid hormone Bos taurus 45-64 95126-2 1979 At concentrations of 0.01 mM or greater, the stimulatory action of prolactin on [3H]-leucine incorporation into a casein-rich protein fraction was also reduced or abolished. Tritium 81-83 prolactin Mus musculus 67-76 467767-5 1979 However, a proportionately greater amount of [3H]-BrdU than [3H]-thymidine was recovered following extensive digestion of renatured complexes with DNase I, even though comparable amounts of DNA were acid-insoluble. Tritium 46-48 deoxyribonuclease 1 Rattus norvegicus 147-154 281572-1 1979 Injection of L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK) at a level of 10 mg/100 g body weight inhibited the incorporation of 3H-labeled amino acids into protein in Morris hepatomas 7777and 9618A2. Tritium 138-140 ribosomal protein L4 Rattus norvegicus 13-16 362945-7 1978 MIF-like activity was detected in MFF-rich supernatants as well as a factor(s) which inhibited 3H-thymidine uptake by giant cells. Tritium 95-97 macrophage migration inhibitory factor Bos taurus 0-3 874493-1 1977 The stereochemistry of tritium atoms at C-7 and C-15. Tritium 23-30 nuclear distribution C, dynein complex regulator Rattus norvegicus 48-52 193558-5 1977 Protein C-14 stimulated the incorporation of [3H]UMP into RNA by approximately 30% when added to naked DNA and homologous RNA polymerase I. Tritium 46-48 anti-Mullerian hormone receptor type 2 Rattus norvegicus 8-12 193558-6 1977 A 30% stimulation of [3H]UMP incorporation into RNA was also found when protein C-14 was added to an E. coli RNA polymerase system containing either E. coli or Novikoff hepatoma DNA. Tritium 22-24 anti-Mullerian hormone receptor type 2 Rattus norvegicus 80-84 266706-1 1977 The binding of neurotensin to synaptic membranes from rat brain was studied at 24 degrees with the use of [3H]neurotensin. Tritium 107-109 neurotensin Rattus norvegicus 15-26 266706-5 1977 Competitive inhibition of [3H]neurotensin binding by partial sequences of neurotensin revealed that the addition of the residue arginine-8 to the neurotensin-(9-13)-pentapeptide increases about 500-fold the relative binding potency, whereas the remaining portion of the NH2-terminal region is mainly responsible for full pharmacological potency; the COOH-terminal leucyl residue is essential for binding. Tritium 27-29 neurotensin Rattus norvegicus 30-41 266706-5 1977 Competitive inhibition of [3H]neurotensin binding by partial sequences of neurotensin revealed that the addition of the residue arginine-8 to the neurotensin-(9-13)-pentapeptide increases about 500-fold the relative binding potency, whereas the remaining portion of the NH2-terminal region is mainly responsible for full pharmacological potency; the COOH-terminal leucyl residue is essential for binding. Tritium 27-29 neurotensin Rattus norvegicus 74-85 266706-5 1977 Competitive inhibition of [3H]neurotensin binding by partial sequences of neurotensin revealed that the addition of the residue arginine-8 to the neurotensin-(9-13)-pentapeptide increases about 500-fold the relative binding potency, whereas the remaining portion of the NH2-terminal region is mainly responsible for full pharmacological potency; the COOH-terminal leucyl residue is essential for binding. Tritium 27-29 neurotensin Rattus norvegicus 74-85 14149-2 1977 The [3H]leucine-labeled in vitro translation product reacted with antibodies specific for albumin and co-migrated with authentic 14C-labeled serum albumin during gel electrophoresis in the presence or absence of sodium dodecyl sufate. Tritium 5-7 albumin Rattus norvegicus 90-97 14149-2 1977 The [3H]leucine-labeled in vitro translation product reacted with antibodies specific for albumin and co-migrated with authentic 14C-labeled serum albumin during gel electrophoresis in the presence or absence of sodium dodecyl sufate. Tritium 5-7 albumin Rattus norvegicus 147-154 1009427-5 1976 The development of the uptake of [3H]choline parallels that of CAT. Tritium 34-36 choline O-acetyltransferase Rattus norvegicus 63-66 977659-6 1976 MSA causes a 2--10-fold increase in ornithine decarboxylase (ODC) activity in 3T3 cells and the dose response curve parallels the dose response curve for [3H] thymidine incorporation into DNA. Tritium 155-157 ornithine decarboxylase, structural 1 Mus musculus 61-64 184819-4 1976 A simple and sensitive assay for 15-hydroxyprostaglandin dehydrogenase was developed based on the stereospecific transfer of the tritium label of tritiated prostaglandins to glutamate by coupling 15-hydroxyprostaglandin dehydrogenase with glutamate dehydrogenase. Tritium 129-136 carbonyl reductase [NADPH] 1 Sus scrofa 33-70 184819-4 1976 A simple and sensitive assay for 15-hydroxyprostaglandin dehydrogenase was developed based on the stereospecific transfer of the tritium label of tritiated prostaglandins to glutamate by coupling 15-hydroxyprostaglandin dehydrogenase with glutamate dehydrogenase. Tritium 129-136 carbonyl reductase [NADPH] 1 Sus scrofa 196-233 8090-1 1976 At pH 8.9 and 37 degrees C the half-times for tritium exchange with the C-2 protons of the histidines of trypsin are 73 days for His-57, and greater than 1000 days for His-40 and His-91. Tritium 46-53 complement C2 Bos taurus 72-75 55272-6 1976 When such a complete ovalbumin [3H]cDNA was synthesized with a specific activity of 10(8) cpm/mug and hyfridized to an excess of chick DNA, the kinetics of hybridization indicated that the cDNA was comprised of a nonrepetitive sequence. Tritium 32-34 ovalbumin (SERPINB14) Gallus gallus 21-30 1081076-5 1976 (3H)-thymidine incorporation experiments demonstrated that both B and T cells were active upon in vitro stimulation with MS-2. Tritium 1-3 minisatellites detected by probe MMS2 Mus musculus 121-125 165190-2 1975 The temperature-activated 4 to 5 S EBP transformation is found to be highly reproducible without loss of [3H]estradiol-binding activity in a buffer containing an excess of [3H]estradiol, 40 mM Tris, 1 mM dithiothreitol, and 1 M urea at pH 7.4. Tritium 106-108 EBP cholestenol delta-isomerase Homo sapiens 35-38 165190-3 1975 The presence of [3H]estradiol is necessary for the 4 to 5 EBP transformation. Tritium 17-19 EBP cholestenol delta-isomerase Homo sapiens 58-61 6027248-0 1967 The relation of spectral changes and tritium exchange reactions to the mechanism of tryptophanase-catalyzed reactions. Tritium 37-44 tryptophan 2,3-dioxygenase Homo sapiens 84-97 16654588-0 1953 Absorption and Metabolism of Tritium Oxide and Tritium Gas by Bean Plants. Tritium 29-36 brain expressed associated with NEDD4 1 Homo sapiens 62-66 33439776-5 2021 We found that TNFalpha elevated Cldn-3 protein expression in tubular epithelial cells (LLC-PK1 and IMCD3) as early as 3h post-treatment. Tritium 118-120 claudin 3 Homo sapiens 32-38 33537927-3 2021 Compared to wild-type hDAT, the maximal velocity (Vmax) of [3H]dopamine uptake was decreased in D381L and Y88F/D206L/H547A, increased in D206L/H547A, and unaltered in D206L. Tritium 60-62 solute carrier family 6 member 3 Homo sapiens 22-26 33537927-5 2021 Mutational effects of hDAT on the transporter conformation were evidenced by attenuation of zinc-induced increased [3H]WIN35,428 binding in D206L/H547A and Y88F/D206A/H547A and enhanced basal MPP+ efflux in D206L/H547A. Tritium 116-118 solute carrier family 6 member 3 Homo sapiens 22-26 33374302-7 2020 Gene knockdown of LAT1 (l-type amino acid transporter 1) and CAT1 (cationic amino acid transporter 1) demonstrated that LAT1 is primarily involved in the transport of [3H]l-tryptophan in both TR-BBB and NSC-34 cell lines. Tritium 168-170 solute carrier family 7 (cationic amino acid transporter, y+ system), member 5 Mus musculus 18-22 32696514-6 2020 The synthesized compounds showed a significant inhibitory activity against 5-LOX, with IC50 values ranging from 0.6 to 4.3 microM, where compounds 3f and 3h were found to be the most potent derivatives, with IC50 values of 0.6 and 1.0 microM, respectively, in comparison with that of zileuton (IC50 = 0.8 microM). Tritium 154-156 lysyl oxidase Homo sapiens 77-80 32298690-0 2020 The selective 5-HT2A receptor agonist 25CN-NBOH: Structure-activity relationship, in vivo pharmacology, and in vitro and ex vivo binding characteristics of [3H]25CN-NBOH. Tritium 157-159 5-hydroxytryptamine (serotonin) receptor 2A Mus musculus 14-29 31721581-2 2020 In this study, we prepared the fully efficacious, nonbiased GPR84 agonist 6-hexylamino-2,4(1H,3H)-pyrimidinedione (6) in tritium-labeled form ([3H]PSB-1584) by hydrogenation of a hexenyl-substituted precursor with tritium gas. Tritium 121-128 G protein-coupled receptor 84 Homo sapiens 60-65 31721581-2 2020 In this study, we prepared the fully efficacious, nonbiased GPR84 agonist 6-hexylamino-2,4(1H,3H)-pyrimidinedione (6) in tritium-labeled form ([3H]PSB-1584) by hydrogenation of a hexenyl-substituted precursor with tritium gas. Tritium 94-96 G protein-coupled receptor 84 Homo sapiens 60-65 31721581-2 2020 In this study, we prepared the fully efficacious, nonbiased GPR84 agonist 6-hexylamino-2,4(1H,3H)-pyrimidinedione (6) in tritium-labeled form ([3H]PSB-1584) by hydrogenation of a hexenyl-substituted precursor with tritium gas. Tritium 214-221 G protein-coupled receptor 84 Homo sapiens 60-65 31721581-8 2020 [3H]6 constitutes the first GPR84 agonist radioligand representing a powerful tool for this poorly investigated GPCR, which has potential as a future drug target. Tritium 1-3 G protein-coupled receptor 84 Homo sapiens 28-33 33132311-7 2020 In addition, co-administration of substrates/inhibitors for Oat3, Oatp1a4, and/or Mrp4, such as benzylpenicillin and cefmetazole, reduced [3H]PGD2 elimination across the BBB. Tritium 139-141 ATP binding cassette subfamily C member 4 Rattus norvegicus 82-86 31167565-6 2019 Despite similar liver LDLr (low-density lipoprotein receptor) expression between genotypes, HSKO mice treated with LDLr-targeting versus control antisense oligonucleotide had slower plasma 3H-CO-HDL decay, reduced selective 3H-CO clearance, and decreased fecal 3H-sterol excretion that was indistinguishable from control mice. Tritium 189-191 low density lipoprotein receptor Mus musculus 115-119 31167565-6 2019 Despite similar liver LDLr (low-density lipoprotein receptor) expression between genotypes, HSKO mice treated with LDLr-targeting versus control antisense oligonucleotide had slower plasma 3H-CO-HDL decay, reduced selective 3H-CO clearance, and decreased fecal 3H-sterol excretion that was indistinguishable from control mice. Tritium 224-226 low density lipoprotein receptor Mus musculus 115-119 31167565-6 2019 Despite similar liver LDLr (low-density lipoprotein receptor) expression between genotypes, HSKO mice treated with LDLr-targeting versus control antisense oligonucleotide had slower plasma 3H-CO-HDL decay, reduced selective 3H-CO clearance, and decreased fecal 3H-sterol excretion that was indistinguishable from control mice. Tritium 224-226 low density lipoprotein receptor Mus musculus 115-119 30863636-6 2019 After intravenous injection into C57BL/6N mice, the plasma half-life of the 3H-triolein for control and C-II-a treated emulsions was 18.3 +- 2.2 min and 14.8 +- 0.1 min, respectively. Tritium 76-78 vacuolar protein sorting 28 Mus musculus 104-110 30863636-7 2019 In apoC-II mutant mice, the plasma half-life of 3H-triolein for injected control and C-II-a treated emulsions was 30.1 +- 0.1 min and 14.8 +- 0.1 min, respectively. Tritium 48-50 vacuolar protein sorting 28 Mus musculus 85-91 29864448-8 2018 alpha7-containing and beta2-containing nAChRs were involved in nicotine-induced [3H]-GABA release in control and mdx synaptosomes. Tritium 81-83 hemoglobin, beta adult minor chain Mus musculus 22-27 29782174-8 2018 Upregulated SLC28A2 mRNA was reflected in elevated uptake of [3H]-adenosine, high-affinity substrate of concentrative nucleoside transporter 2. Tritium 62-65 solute carrier family 28 member 2 Homo sapiens 12-19 29712723-11 2018 1 eq of IsoLG decreased HDL-mediated [3H]cholesterol efflux from macrophages via ABCA1, which corresponded to a decrease in HDL-apoA-I exchange from 47.4% to only 24.8%. Tritium 38-40 ATP binding cassette subfamily A member 1 Homo sapiens 81-86 29599133-4 2018 LAL-deficient macrophages loaded with [3H]-cholesteryl oleate-labeled acetylated low-density lipoprotein showed impaired efflux of released [3H]-cholesterol to apoA-I (apolipoprotein A-I), with normalization of [3H]-cholesteryl ester levels and partial correction of ABCA1 expression and cholesterol efflux to apoA-I when treated with exogenous rhLAL (recombinant human LAL protein). Tritium 39-41 ATP binding cassette subfamily A member 1 Homo sapiens 267-272 28875261-4 2018 Here we report that [3H]-DPA-713 in the presence of cholesterol or PK11195 has an accelerated dissociation rate from TSPO in platelets isolated from individuals with the T/T genotype. Tritium 21-23 translocator protein Homo sapiens 117-121 29263400-7 2017 However, although a radiolabelled form of a related antagonist, [3H]G9543, was able to bind with high affinity to GPR84, this was not competed for by increasing concentrations of either decanoic acid or 3,3"-diindolylmethane and was not affected adversely by mutation of arginine172. Tritium 65-67 G protein-coupled receptor 84 Homo sapiens 114-119 29074589-3 2017 APPROACH AND RESULTS: During in vitro incubation of human serum, nHDL-[3H]FC and [14C]PL rapidly transfer to HDL and low-density lipoproteins (t1/2=2-7 minutes), whereas nHDL-[125I]apo AI transfers solely to HDL (t1/2<10 minutes) and to the lipid-free form (t1/2>480 minutes). Tritium 71-73 apolipoprotein A-I Mus musculus 181-187 28923416-4 2017 The sigma1R antagonist BD1063 (100nM), amplified the effects of quinpirole (10 and 100nM) on K+-evoked [3H]-DA, but not glutamate, release. Tritium 104-106 sigma non-opioid intracellular receptor 1 Rattus norvegicus 4-11 28289968-0 2017 Synthesis and In Vitro and In Vivo Evaluation of [3H]LRRK2-IN-1 as a Novel Radioligand for LRRK2. Tritium 50-52 inversion, Chr 17 Mus musculus 59-63 28289968-4 2017 PROCEDURES: [3H]LRRK2-IN-1 was prepared with high radiochemical purity (>99 %) and a specific activity of 41 Ci/mmol via tritium/hydrogen (T/H) exchange using Crabtree"s catalyst. Tritium 13-15 inversion, Chr 17 Mus musculus 22-26 28289968-4 2017 PROCEDURES: [3H]LRRK2-IN-1 was prepared with high radiochemical purity (>99 %) and a specific activity of 41 Ci/mmol via tritium/hydrogen (T/H) exchange using Crabtree"s catalyst. Tritium 124-131 inversion, Chr 17 Mus musculus 22-26 28289968-7 2017 RESULTS: In vitro binding studies demonstrated a saturable binding site for [3H]LRRK2-IN-1 in rat kidney, rat brain striatum and human brain striatum with K d of 26 +- 3 and 43 +- 8, 48 +- 2 nM, respectively. Tritium 77-79 inversion, Chr 17 Mus musculus 86-90 28289968-10 2017 In in vivo biodistribution and blocking studies in mice, co-administration with Pf-06447475 (10 mg/kg) reduced the uptake of [3H]LRRK2-IN-1 (%ID/g) by 50-60% in the kidney or brain. Tritium 126-128 inversion, Chr 17 Mus musculus 135-139 29167643-6 2017 Instead, [3H]acetylcholine release was attenuated by sodium fluoroacetate (5 mM), carbenoxolone (10 muM) and A438079 (3 muM), which prevent activation of glial cells, pannexin-1 hemichannels and P2X7 receptors, respectively. Tritium 10-12 Pannexin 1 Rattus norvegicus 167-177 28859474-10 2017 The binding of a dopamine reuptake inhibitor, [3H]-WIN35,428, to DAT is significantly disrupted by aggregated-7-(10,10)CNTs and hence improve the ability to transport dopamine. Tritium 47-49 solute carrier family 6 member 3 Homo sapiens 65-68 28824395-6 2017 Radioligand binding assays of [3H]WIN35,428 demonstrated that the number of striatal DAT binding sites was inversely correlated with increasing BMI (r = -0.47; p < 0.01). Tritium 31-33 solute carrier family 6 member 3 Homo sapiens 85-88 28623359-6 2017 Compared to a competitive DAT inhibitor indatraline, both SRI-compounds displayed a similar decrease (30%) in IC50 for inhibition of [3H]DA uptake by cocaine in WT hDAT. Tritium 134-136 sorcin Homo sapiens 58-61 28623359-7 2017 The addition of SRI-20041 or SRI-30827 following cocaine slowed the dissociation rate of [3H]WIN35,428 binding in WT hDAT relative to cocaine alone. Tritium 90-92 sorcin Homo sapiens 16-19 28623359-7 2017 The addition of SRI-20041 or SRI-30827 following cocaine slowed the dissociation rate of [3H]WIN35,428 binding in WT hDAT relative to cocaine alone. Tritium 90-92 sorcin Homo sapiens 29-32 28623359-9 2017 SRI-30827 attenuated Tat-induced inhibition of [3H]WIN35,428 binding. Tritium 48-50 sorcin Homo sapiens 0-3 28315326-4 2017 In this study, we purified the macrodomain-containing PARP14 enzyme and established an assay for detecting the auto-ribosylation activity of PARP14 using RapidFire high-throughput mass spectrometry and immunoradiometric assay using [3H]NAD+. Tritium 233-235 poly(ADP-ribose) polymerase family member 14 Homo sapiens 54-60 28315326-4 2017 In this study, we purified the macrodomain-containing PARP14 enzyme and established an assay for detecting the auto-ribosylation activity of PARP14 using RapidFire high-throughput mass spectrometry and immunoradiometric assay using [3H]NAD+. Tritium 233-235 poly(ADP-ribose) polymerase family member 14 Homo sapiens 141-147 28131852-5 2017 The inhibitory effect of Tween 20, Tween 60 and Tween 80 on OCT2-mediated [3H]-MPP+ uptake in the human renal proximal tubular cells (RPTEC/TERT1 cells) was found and the IC50 values was similar to heterologous OCT2 expressing cells. Tritium 75-77 POU class 2 homeobox 2 Homo sapiens 60-64 28087695-8 2017 [14C]Choline and [3H]palmitate tracking shows that SMS1 overexpression apparently affects the partitioning of palmitate-enriched diacylglycerol between the phosphatidylcholine and triacylglycerol pathways, to the benefit of the former. Tritium 18-20 sphingomyelin synthase 1 Homo sapiens 51-55 27812757-11 2016 With OAT2, dantrolene and 5-OH dantrolene slightly decreased the pre-equilibrated [3H]cGMP content. Tritium 83-85 solute carrier family 22 member 7 Homo sapiens 5-9 27214288-0 2016 Measurement of tritium in the Sava and Danube Rivers. Tritium 15-22 T-box transcription factor T Homo sapiens 30-34 27214288-3 2016 The authors present the tritium measurement results taken over several years from the Sava and Danube Rivers, and groundwater. Tritium 24-31 T-box transcription factor T Homo sapiens 86-90 27214288-8 2016 The Krsko NPP discharged 62 TBq of tritium into the River Sava over a period of 6 years (23% of permitted activity, 45 TBq per year). Tritium 35-42 T-box transcription factor T Homo sapiens 58-62 27214288-9 2016 The natural level of tritium in the Sava River and groundwater is 0.3-1 Bq/l and increases when discharges exceed 1 TBq per month. Tritium 21-28 T-box transcription factor T Homo sapiens 36-40 27376201-7 2016 RESULTS: A concentration-dependent inhibitory effect of different corticosteroids on the [(3)H]NE and [(3)H]DA uptake via OCT3 was illustrated in vitro. Tritium 90-94 solute carrier family 22 member 3 Homo sapiens 122-126 26554626-9 2016 Conversely, P-gp/Bcrp protein expressions remained unchanged after naloxone administration, and brain uptake of [3H]-verapamil (P-gp) and [3H]-mitoxantrone (Bcrp) was not altered. Tritium 113-115 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 128-132 26619345-3 2015 Upregulation can be measured using HEK293 (293) cells that stably express alpha4 and beta2 subunits using quantification of [3H]epibatidine ([3H]Eb) binding to measure mature receptors. Tritium 125-127 immunoglobulin binding protein 1 Homo sapiens 74-80 26619345-3 2015 Upregulation can be measured using HEK293 (293) cells that stably express alpha4 and beta2 subunits using quantification of [3H]epibatidine ([3H]Eb) binding to measure mature receptors. Tritium 142-144 immunoglobulin binding protein 1 Homo sapiens 74-80 26032487-3 2015 Among these compounds, compound 3h exhibited the most potent inhibitory activities against VEGFR2 tyrosine kinase and cell proliferation, with the IC50 values of 2.89 nm (for VEGFR2) and 0.25 mum (for MCF-7), which were comparable with the control compound. Tritium 32-34 kinase insert domain receptor Homo sapiens 175-181 25580950-4 2015 In this work, human embryonic kidney 293 cells were co-transfected with DAT constructs possessing differential binding affinities for the phenyltropane cocaine analog, [3H]WIN35,428. Tritium 169-171 solute carrier family 6 member 3 Homo sapiens 72-75 25772534-2 2015 In a study by Zhen and colleagues highlighted by this Editorial in the current issue of Journal of Neurochemistry, the combination of mutant and wild-type dopamine transporter (DAT) has been used to establish the cooperation between transporter protomers; the DAT mutant version has an altered affinity for the radiolabelled inhibitor [3H]CFT. Tritium 336-338 solute carrier family 6 member 3 Homo sapiens 155-175 25772534-2 2015 In a study by Zhen and colleagues highlighted by this Editorial in the current issue of Journal of Neurochemistry, the combination of mutant and wild-type dopamine transporter (DAT) has been used to establish the cooperation between transporter protomers; the DAT mutant version has an altered affinity for the radiolabelled inhibitor [3H]CFT. Tritium 336-338 solute carrier family 6 member 3 Homo sapiens 177-180 25772534-2 2015 In a study by Zhen and colleagues highlighted by this Editorial in the current issue of Journal of Neurochemistry, the combination of mutant and wild-type dopamine transporter (DAT) has been used to establish the cooperation between transporter protomers; the DAT mutant version has an altered affinity for the radiolabelled inhibitor [3H]CFT. Tritium 336-338 solute carrier family 6 member 3 Homo sapiens 260-263 25826533-4 2015 Fatty acid amide hydrolase (FAAH) and monoaclyglycerol lipase (MAGL) activity was assessed by [3H]AEA and [3H]2-AG hydrolysis, respectively. Tritium 95-97 monoglyceride lipase Homo sapiens 38-61 25826533-4 2015 Fatty acid amide hydrolase (FAAH) and monoaclyglycerol lipase (MAGL) activity was assessed by [3H]AEA and [3H]2-AG hydrolysis, respectively. Tritium 95-97 monoglyceride lipase Homo sapiens 63-67 25826533-4 2015 Fatty acid amide hydrolase (FAAH) and monoaclyglycerol lipase (MAGL) activity was assessed by [3H]AEA and [3H]2-AG hydrolysis, respectively. Tritium 107-109 monoglyceride lipase Homo sapiens 38-61 25826533-4 2015 Fatty acid amide hydrolase (FAAH) and monoaclyglycerol lipase (MAGL) activity was assessed by [3H]AEA and [3H]2-AG hydrolysis, respectively. Tritium 107-109 monoglyceride lipase Homo sapiens 63-67 25446673-5 2015 RESULTS: In Chinese hamster ovary (CHO) cell membranes transfected with rat KOR (CHO-rKOR) rimonabant inhibited KOR agonist [3H]U69593 binding in the micromolar range in competition binding experiments and specifically reduced KOR basal activity at lower micromolar concentrations in [35S]GTPgammaS binding assays. Tritium 125-127 opioid receptor, kappa 1 Mus musculus 76-79 25446673-5 2015 RESULTS: In Chinese hamster ovary (CHO) cell membranes transfected with rat KOR (CHO-rKOR) rimonabant inhibited KOR agonist [3H]U69593 binding in the micromolar range in competition binding experiments and specifically reduced KOR basal activity at lower micromolar concentrations in [35S]GTPgammaS binding assays. Tritium 125-127 opioid receptor, kappa 1 Mus musculus 86-89 25446673-5 2015 RESULTS: In Chinese hamster ovary (CHO) cell membranes transfected with rat KOR (CHO-rKOR) rimonabant inhibited KOR agonist [3H]U69593 binding in the micromolar range in competition binding experiments and specifically reduced KOR basal activity at lower micromolar concentrations in [35S]GTPgammaS binding assays. Tritium 125-127 opioid receptor, kappa 1 Mus musculus 86-89 25710042-7 2015 Therefore, we have tested the interaction of human OAT2 with various substances using tritium-labeled cGMP. Tritium 86-93 solute carrier family 22 member 7 Homo sapiens 51-55 25495612-3 2014 The compounds were screened by using tritium-labeled adenosine 3",5"-cyclic monophosphate ([(3)H]-cGMP) as a substrate for inhibitory affinities against phosphodiesterase-9A (PDE9A), which is a potential target for the treatment of diabetes and Alzheimer"s disease. Tritium 37-44 phosphodiesterase 9A Homo sapiens 153-173 25495612-3 2014 The compounds were screened by using tritium-labeled adenosine 3",5"-cyclic monophosphate ([(3)H]-cGMP) as a substrate for inhibitory affinities against phosphodiesterase-9A (PDE9A), which is a potential target for the treatment of diabetes and Alzheimer"s disease. Tritium 91-98 phosphodiesterase 9A Homo sapiens 153-173 25495612-3 2014 The compounds were screened by using tritium-labeled adenosine 3",5"-cyclic monophosphate ([(3)H]-cGMP) as a substrate for inhibitory affinities against phosphodiesterase-9A (PDE9A), which is a potential target for the treatment of diabetes and Alzheimer"s disease. Tritium 91-98 phosphodiesterase 9A Homo sapiens 175-180 25446845-3 2014 Exogenous IL-10 reversed oxLDL-inhibited chemotactic macrophage numbers by 48.18 +- 4.93% (3h), 64.8 +- 5.61% (6h) and 63.66 +- 3.05% (12h), and pretreated with SB203580 (P38 signaling inhibitor) in macrophage, oxLDL could not inhibit macrophage emigration. Tritium 91-93 interleukin 10 Homo sapiens 10-15 25223904-14 2014 We observed a dramatic decrease in HMGB1 immunostaining at 3h after hyperglycemic ischemia that gradually returned to control levels. Tritium 59-61 high mobility group box 1 Rattus norvegicus 35-40 25152469-7 2014 DFX reduced the expression level of active caspase-3 and increased the expression level of HIF-1alpha in astrocytes induced by 3h of OGD, but had no effect on aquaporin-4 (AQP4) expression. Tritium 127-129 hypoxia inducible factor 1 subunit alpha Rattus norvegicus 91-101 25264156-5 2014 VEGF was administered intracerebroventricularly 3h after reperfusion. Tritium 48-50 vascular endothelial growth factor A Mus musculus 0-4 24953854-8 2014 Absolute number of CD11b(+) and CD11b(+)Gr-1(+) cells was enhanced in R1 region from 3h to 72 h. B16F10 tumor growth was significantly increased by intravenous injection of CD11b(+) BMCs. Tritium 85-87 integrin subunit alpha M Homo sapiens 19-24 24953854-8 2014 Absolute number of CD11b(+) and CD11b(+)Gr-1(+) cells was enhanced in R1 region from 3h to 72 h. B16F10 tumor growth was significantly increased by intravenous injection of CD11b(+) BMCs. Tritium 85-87 integrin subunit alpha M Homo sapiens 32-37 24953854-8 2014 Absolute number of CD11b(+) and CD11b(+)Gr-1(+) cells was enhanced in R1 region from 3h to 72 h. B16F10 tumor growth was significantly increased by intravenous injection of CD11b(+) BMCs. Tritium 85-87 integrin subunit alpha M Homo sapiens 32-37 25000292-6 2014 Ligand binding assay with guinea pig liver cytosols revealed that both (+)-KET and (-)-KET are weak ligands of AhR that displaced [3H]-TCDD with comparable potency. Tritium 131-133 aryl hydrocarbon receptor Cavia porcellus 111-114 24746628-3 2014 As revealed by quantitative immunocytochemistry, the severe hypobaric hypoxia (180 Torr, 3h) substantially down-regulated the levels of pCREB and BDNF in cortical neurons assessed 24h after the treatment. Tritium 89-91 brain-derived neurotrophic factor Rattus norvegicus 146-150 24891283-0 2014 Perinatal hypoxia: different effects of the inhibitors of GABA transporters GAT1 and GAT3 on the initial velocity of [3H]GABA uptake by cortical, hippocampal, and thalamic nerve terminals. Tritium 118-120 solute carrier family 6 member 12 Rattus norvegicus 76-80 24891283-0 2014 Perinatal hypoxia: different effects of the inhibitors of GABA transporters GAT1 and GAT3 on the initial velocity of [3H]GABA uptake by cortical, hippocampal, and thalamic nerve terminals. Tritium 118-120 solute carrier family 6 member 11 Rattus norvegicus 85-89 24273061-10 2014 A significant portion of the GlyT2-mediated release of [3H]GABA (about 50% of the external Ca(2+)-independent release) occurred by reversal of GABA GAT1 transporters. Tritium 56-58 glycoprotein alpha-galactosyltransferase 1 (inactive) Homo sapiens 29-34 23259992-5 2013 Jejunal uptake of [(3)H]fMet-Leu-Phe was specific for PepT1 and saturable with an intrinsic K(0.5) of 1.6 mM. Tritium 18-23 solute carrier family 15 (oligopeptide transporter), member 1 Mus musculus 54-59 23174481-6 2013 Zebrafish larvae exposed to Cd for 3h showed increased glutathione S-transferase pi (gst pi), glutamate-cysteine ligase catalytic subunit (gclc), heme oxygenase 1 (hmox1) and peroxiredoxin 1 (prdx1) mRNA levels indicative of Nrf2 activation, and which were blocked by morpholino-mediated Nrf2 knockdown. Tritium 35-37 glutathione S-transferase pi 1.2 Danio rerio 55-83 23174481-6 2013 Zebrafish larvae exposed to Cd for 3h showed increased glutathione S-transferase pi (gst pi), glutamate-cysteine ligase catalytic subunit (gclc), heme oxygenase 1 (hmox1) and peroxiredoxin 1 (prdx1) mRNA levels indicative of Nrf2 activation, and which were blocked by morpholino-mediated Nrf2 knockdown. Tritium 35-37 glutathione S-transferase pi 1.2 Danio rerio 85-91 23174481-6 2013 Zebrafish larvae exposed to Cd for 3h showed increased glutathione S-transferase pi (gst pi), glutamate-cysteine ligase catalytic subunit (gclc), heme oxygenase 1 (hmox1) and peroxiredoxin 1 (prdx1) mRNA levels indicative of Nrf2 activation, and which were blocked by morpholino-mediated Nrf2 knockdown. Tritium 35-37 heme oxygenase 1a Danio rerio 146-162 23174481-6 2013 Zebrafish larvae exposed to Cd for 3h showed increased glutathione S-transferase pi (gst pi), glutamate-cysteine ligase catalytic subunit (gclc), heme oxygenase 1 (hmox1) and peroxiredoxin 1 (prdx1) mRNA levels indicative of Nrf2 activation, and which were blocked by morpholino-mediated Nrf2 knockdown. Tritium 35-37 heme oxygenase 1a Danio rerio 164-169 23320076-0 2013 Increases in [3H]muscimol and [3H]flumazenil binding in the dorsolateral prefrontal cortex in schizophrenia are linked to alpha4 and gamma2S mRNA levels respectively. Tritium 31-33 immunoglobulin binding protein 1 Homo sapiens 122-128 22929013-5 2012 The low-output tonic opener NMJs in crayfish exposed to 4 muM bafilomycin A1 and 20-Hz continuous stimulation decreased the EPSP amplitude to 50% in ~30 min with controls lasting 3h. Tritium 179-181 anon-A1 Drosophila melanogaster 74-83 23127014-0 2012 Diagnosing radiative shocks from deuterium and tritium implosions on NIF. Tritium 47-54 S100 calcium binding protein A9 Homo sapiens 69-72 22483869-4 2012 Studies with synaptic vesicles from rat substantia nigra revealed that VMAT-2 uptake of 3H-aminochrome 6.3 +- 0.4nmol/min/mg was similar to dopamine uptake 6.2 +- 0.3nmol/min/mg that which were dependent on ATP. Tritium 88-90 solute carrier family 18 member A2 Rattus norvegicus 71-77 22365949-4 2012 The serum DNase I activity levels within 3h after chest pain and the absolute median value of percentage differences in serum DNase I activity levels from admission to 3h after hospitalization in the UAP/NSTEMI patients was significantly higher than those in the CPS patients. Tritium 41-43 deoxyribonuclease 1 Homo sapiens 10-17 22365949-4 2012 The serum DNase I activity levels within 3h after chest pain and the absolute median value of percentage differences in serum DNase I activity levels from admission to 3h after hospitalization in the UAP/NSTEMI patients was significantly higher than those in the CPS patients. Tritium 168-170 deoxyribonuclease 1 Homo sapiens 126-133 22424937-7 2012 Serum IL-6 level peaked at 3h (1.89 +- 0.10 ng/ml) and serum TNF-alpha peaked at 1h (1.11 +- 0.01 ng/ml). Tritium 27-29 interleukin 6 Canis lupus familiaris 6-10 22242452-4 2011 The compounds 3e, 3h, 3j and 31 showed Cdc25A phosphatase inhibitory activity of 60.6%, 58.6%, 51.4% and 98.4% respectively at the test concentration of 20 microg x mL(-1), and among them 31 had inhibition rate of 86.97% even at the concentration as low as 5 microg x mL(-1), indicating worthy to be future studied. Tritium 18-20 cell division cycle 25A Homo sapiens 39-45 21787820-5 2011 The best synthesis conditions were obtained after E. coli cultivation at 26 C for 3h, which allowed for the production of an rAp-S-enriched fraction containing the peptide at 249muM. Tritium 82-84 LDL receptor related protein associated protein 1 Rattus norvegicus 125-128 21737633-7 2011 [3H]FLT uptake showed negative moderate correlation with dNT1/GAPDH levels (r=-0.563; p<0.01), but no significance with TK1/GAPDH levels. Tritium 1-3 Neurotrophin 1 Drosophila melanogaster 57-61 20192946-1 2011 Specific binding of [3H]MK801 to N-methyl-D-aspartate (NMDA) receptors in the frontal cortex and hippocampus (CA1 and gyrus dentatus) was measured by receptor autoradiography in 16 Caucasian chronic alcohol consumers free of clinical manifestations of alcoholism, and compared with 16 Caucasian control subjects. Tritium 21-23 carbonic anhydrase 1 Homo sapiens 110-113 21914170-6 2011 RESULTS: CNP reduced NO and PGE2 release and partially restored [3H]-thymidine and 35SO4 incorporation in constructs cultured with IL-1beta. Tritium 65-67 natriuretic peptide C Homo sapiens 9-12 21129948-7 2011 RESULTS: The median length of oxytocin infusion was 5.3h. Tritium 54-56 oxytocin/neurophysin I prepropeptide Homo sapiens 30-38 21731704-0 2011 Pharmacological characterization of [3H]CHIBA-3007 binding to glycine transporter 1 in the rat brain. Tritium 37-39 solute carrier family 6 member 9 Rattus norvegicus 62-83 21731704-5 2011 The specific binding of [3H]CHIBA-3007 was inhibited by a number of GlyT-1 inhibitors, such as CHIBA-3007, desmethyl-CHIBA-3007, CHIBA-3008, SSR504734, NFPS/ALX5407, LY2365109 and Org24598, consistent with the pharmacological profiles of GlyT-1 inhibitors. Tritium 25-27 solute carrier family 6 member 9 Rattus norvegicus 68-74 21731704-5 2011 The specific binding of [3H]CHIBA-3007 was inhibited by a number of GlyT-1 inhibitors, such as CHIBA-3007, desmethyl-CHIBA-3007, CHIBA-3008, SSR504734, NFPS/ALX5407, LY2365109 and Org24598, consistent with the pharmacological profiles of GlyT-1 inhibitors. Tritium 25-27 solute carrier family 6 member 9 Rattus norvegicus 238-244 20797845-9 2010 Pharmacokinetics of L19-IL2 was dose proportional over the tested range, with a terminal half-life of 2-3h. Tritium 104-106 immunoglobulin kappa variable 1-12 Homo sapiens 20-23 20691713-2 2010 Here we report on the use of two novel radioligands, [(3)H]-SB-733993 and [(3)H]-GSK931145, for the characterisation of GlyT-1 in both cells and native tissue. Tritium 53-58 solute carrier family 6 member 9 Rattus norvegicus 120-126 20691713-2 2010 Here we report on the use of two novel radioligands, [(3)H]-SB-733993 and [(3)H]-GSK931145, for the characterisation of GlyT-1 in both cells and native tissue. Tritium 54-58 solute carrier family 6 member 9 Rattus norvegicus 120-126 21057870-7 2010 However, when cell homogenates were incubated with glucosylceramide in the presence of UDP-[(3)H]Gal, Lac-Cer synthase activity in B4galt5 ( +/- ) - and B4galt5 ( -/- ) -derived MEF cells decreased to 41% and 11% of that of B4galt5 ( +/+ )-derived MEF cells. Tritium 92-96 UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 Mus musculus 131-160 20170649-9 2010 The preferential alpha(1B)-adrenoceptor antagonist, AH11110A, the alpha2-adrenoceptor agonist, RWJ52353, and the adrenergic neurotoxin DSP-4 likewise affected [3H]MPP+ transport, in an OCT-subtype and species-dependent manner. Tritium 160-162 adrenoceptor alpha 1B Homo sapiens 17-39 20629311-4 2010 The effects of CD4+ CD25high CCR6+ regulatory T cells on the proliferation of CD4+ CD25- T cells also were detected by 3H-incorporation assay. Tritium 119-121 interferon stimulated exonuclease gene 20 Homo sapiens 20-24 20629311-4 2010 The effects of CD4+ CD25high CCR6+ regulatory T cells on the proliferation of CD4+ CD25- T cells also were detected by 3H-incorporation assay. Tritium 119-121 C-C motif chemokine receptor 6 Homo sapiens 29-33 20629311-4 2010 The effects of CD4+ CD25high CCR6+ regulatory T cells on the proliferation of CD4+ CD25- T cells also were detected by 3H-incorporation assay. Tritium 119-121 interferon stimulated exonuclease gene 20 Homo sapiens 83-87 20460875-4 2010 The role and function of hBSEP have been investigated by the examination of the ATP-dependent transport of radioactive isotopically (RI)-labeled bile acid such as a tritium labeled taurocholic acid, in membrane vesicles obtained from hBSEP-expressing cells. Tritium 165-172 ATP binding cassette subfamily B member 11 Homo sapiens 25-30 19377069-4 2009 METHODS AND RESULTS: [3H]-Cholesterol- and [3H]-methylcholine chloride-labelled murine macrophages treated with EP 80317 showed a significant increase in cholesterol and phospholipid efflux to both apolipoprotein A-I and high-density lipoprotein in a CD36-dependent manner. Tritium 22-24 apolipoprotein A-I Mus musculus 198-216 19428788-4 2009 The stimulatory effect of WIN55,212-2 on the evoked tritium efflux was inhibited by the selective CB1 cannabinoid receptor antagonist N-(piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251, 100 nM) and by the GABA(A) receptor antagonist bicuculline (10 microM). Tritium 52-59 cannabinoid receptor 1 Rattus norvegicus 98-101 19409113-7 2009 11beta-HSD2 protein levels in Leydig cells were assayed by Western Blot and 11beta-HSD2 enzyme activity was determined by calculating the ratio of conversion of [3H]CORT to [3H]11-dehydrocorticosterone by 24 h after stimulation with 20 ng/ml LH. Tritium 162-164 hydroxysteroid 11-beta dehydrogenase 2 Rattus norvegicus 76-87 19167238-4 2009 Endotoxin administration alone induced a 3-, 12- and 5-fold increase in plasma concentrations of TNF-alpha, IL-6 and IL-10, respectively, 3h after LPS challenge. Tritium 138-140 interleukin 10 Homo sapiens 117-122 18599737-6 2008 This CEH-mediated increased movement of [3H]cholesterol from macrophages to bile acids and feces was significantly attenuated in SR-BI(-/-) mice. Tritium 41-43 epoxide hydrolase 2, cytoplasmic Mus musculus 5-8 19383336-5 2008 In contrast, in OVCA, SnoN levels were elevated 3h post-stimulation potentially as a result of inhibition of the proteosome. Tritium 48-50 SKI like proto-oncogene Homo sapiens 22-26 18395436-6 2008 GOx was immobilized by physical adsorption (soaking films in GOx solution containing 1000 units for 3h). Tritium 100-102 hydroxyacid oxidase 1 Homo sapiens 0-3 18436710-10 2008 However, dynamin 2 knockdown caused a significant increase in the overall levels of intact [3H]AEA associated with the cells, suggesting that trafficking of [3H]AEA to FAAH had been disrupted. Tritium 92-94 dynamin 2 Rattus norvegicus 9-18 18436710-10 2008 However, dynamin 2 knockdown caused a significant increase in the overall levels of intact [3H]AEA associated with the cells, suggesting that trafficking of [3H]AEA to FAAH had been disrupted. Tritium 158-160 dynamin 2 Rattus norvegicus 9-18 18038207-1 2008 The paucity of information on the presence of the dopamine transporter (DAT) in blood cells, prompted us to explore it in human resting lymphocytes by means of the binding of 3H-WIN 35,428, a compound which is currently considered the most selective ligand for labelling this protein, and by means of the specific reuptake of 3H-dopamine (3H-DA). Tritium 175-177 solute carrier family 6 member 3 Homo sapiens 50-70 18038207-1 2008 The paucity of information on the presence of the dopamine transporter (DAT) in blood cells, prompted us to explore it in human resting lymphocytes by means of the binding of 3H-WIN 35,428, a compound which is currently considered the most selective ligand for labelling this protein, and by means of the specific reuptake of 3H-dopamine (3H-DA). Tritium 175-177 solute carrier family 6 member 3 Homo sapiens 72-75 18258687-7 2008 Furthermore, TFF3-induced increases in [3H]thymidine incorporation in rat islets cultured in serum was blocked by overexpression of a dominant-negative Akt protein or treatment with triciribine, an Akt inhibitor. Tritium 40-42 trefoil factor 3 Rattus norvegicus 13-17 18054208-6 2008 METHODS: The equilibrium binding parameters of the specific TSPO ligand [3H]PK 11195 were estimated on platelet membranes from 40 adult outpatients with DSM-IV diagnosis of MDD, with or without separation anxiety symptoms, and 20 healthy controls. Tritium 73-75 translocator protein Homo sapiens 60-64 18249546-6 2008 In receptor binding studies with the radioligand [(3)H]-deltorphine II only ent-3 showed considerable delta receptor affinity (K(i)=740 nM). Tritium 50-54 solute carrier family 29 member 3 Homo sapiens 76-81 18162287-10 2008 Primary cultures of rat granulosa cells responded to purified hGDF9 with an increase in DNA synthesis as measured by [3H]-thymidine uptake. Tritium 118-120 growth differentiation factor 9 Homo sapiens 62-67 18096387-3 2008 Replacement of a cyano group by a five-membered heterocycle produced compounds inhibiting the binding of [(3)H]-LY354740 to rat mGluR2 with low nanomolar affinity and consistent functional effect at both mGluR2 and mGluR3. Tritium 105-112 glutamate receptor, ionotropic, AMPA3 (alpha 3) Mus musculus 215-221 17531458-2 2008 Our group has previously shown that both reduced folate carrier (RFC1) and folate receptor alpha (FRalpha) seem to be involved in the uptake of [3H]folic acid ([3H]FA) by a human trophoblast cell line (BeWo) and by human primary cultured cytotrophoblasts. Tritium 145-147 replication factor C subunit 1 Homo sapiens 65-69 17531458-2 2008 Our group has previously shown that both reduced folate carrier (RFC1) and folate receptor alpha (FRalpha) seem to be involved in the uptake of [3H]folic acid ([3H]FA) by a human trophoblast cell line (BeWo) and by human primary cultured cytotrophoblasts. Tritium 161-163 replication factor C subunit 1 Homo sapiens 65-69 17531458-6 2008 On the other hand, [3H]FA uptake was significantly increased by chronic exposure to xanthohumol, quercetin or isoxanthohumol (0.1-10 microM), and this increase does not seem to result from changes in the level of RFC1 or FRalpha gene expression. Tritium 20-22 replication factor C subunit 1 Homo sapiens 213-217 18068762-1 2008 [(3)H]Tyr-Tic-(2S,3R)-beta-MePhe-Phe-OH (where Tic: 1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid) with a specific radioactivity of 53.7 Ci/mmol was synthesized and characterized in receptor binding assays at 25 degrees C in rat brain membranes. Tritium 0-5 aryl hydrocarbon receptor nuclear translocator-like Rattus norvegicus 10-13 18068762-1 2008 [(3)H]Tyr-Tic-(2S,3R)-beta-MePhe-Phe-OH (where Tic: 1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid) with a specific radioactivity of 53.7 Ci/mmol was synthesized and characterized in receptor binding assays at 25 degrees C in rat brain membranes. Tritium 0-5 aryl hydrocarbon receptor nuclear translocator-like Rattus norvegicus 47-50 18069127-0 2008 A novel TRH analog, Glp-Asn-Pro-D-Tyr-D-TrpNH2, binds to [3H][3-Me-His2]TRH-labelled sites in rat hippocampus and cortex but not pituitary or heterologous cells expressing TRHR1 or TRHR2. Tritium 58-60 motilin receptor Rattus norvegicus 181-186 18027973-8 2007 In addition, and for the first time, high-affinity binding of a radioligand to hNav1.7 channels (Kd = 1.6 nM) was achieved with [3H]BNZA, providing an additional means for identifying selective Nav1.7 channel inhibitors. Tritium 129-131 sodium voltage-gated channel alpha subunit 9 Homo sapiens 79-86 18027973-8 2007 In addition, and for the first time, high-affinity binding of a radioligand to hNav1.7 channels (Kd = 1.6 nM) was achieved with [3H]BNZA, providing an additional means for identifying selective Nav1.7 channel inhibitors. Tritium 129-131 sodium voltage-gated channel alpha subunit 9 Homo sapiens 80-86 17631923-4 2007 Deletion of the alpha7, beta2 or beta4 nicotinic receptor subunit genes identifies highly expressed subtypes with relatively low affinity for [3H]epibatidine. Tritium 143-145 hemoglobin, beta adult minor chain Mus musculus 24-29 17556759-5 2007 In vitro-expressed AhR1 and AhR2 isoforms from both albatross and cormorant exhibited specific binding to [3H]TCDD, as assessed by velocity sedimentation. Tritium 107-109 LOW QUALITY PROTEIN: uncharacterized protein LOC104051548 Phalacrocorax carbo 28-32 17541020-5 2007 When injected into abca1+/+ mice, return of 3H-cholesterol from labeled abca1-/- macrophages to serum, liver, bile, and feces was reduced by 50% (P=0.01) compared with control. Tritium 44-46 ATP-binding cassette, sub-family A (ABC1), member 1 Mus musculus 19-24 17541020-5 2007 When injected into abca1+/+ mice, return of 3H-cholesterol from labeled abca1-/- macrophages to serum, liver, bile, and feces was reduced by 50% (P=0.01) compared with control. Tritium 44-46 ATP-binding cassette, sub-family A (ABC1), member 1 Mus musculus 72-77 17541020-6 2007 When labeled wild-type macrophages were injected into abca1-/- mice, as compared with wild-type mice, the return of 3H-cholesterol to serum, liver, bile, and feces was also reduced. Tritium 116-118 ATP-binding cassette, sub-family A (ABC1), member 1 Mus musculus 54-59 17512915-6 2007 [3H]-L-685,458 binding sites in the adult brain were differentially distributed across regions and laminas, with heavy binding localized to the olfactory glomeruli, hippocampal CA3 and cerebellar molecular layer, and moderate binding in the cerebral cortex, amygdala and selected subcortical regions. Tritium 1-3 carbonic anhydrase 3 Rattus norvegicus 177-180 17397826-9 2007 Ibu-am5 inhibited the binding of [3H]-CP55,940 to rat brain CB1 and human CB2 cannabinoid receptors more potently than ibuprofen, but the increase in potency was less than the corresponding increase in potency seen for inhibition of FAAH activity. Tritium 34-36 cannabinoid receptor 1 Rattus norvegicus 60-63 17324121-8 2007 Importantly, inhibition of hyaluronan binding to its receptor CD44 by the monoclonal antibody Hermes-1, inhibited PDGF-BB-stimulated [3H]thymidine incorporation of dermal fibroblasts. Tritium 134-136 CD44 molecule (Indian blood group) Homo sapiens 62-66 17239354-3 2007 RESULTS: The [3H]pirenzepine binding was decreased in the dentate gyrus (p < .05), CA3 (p < .01), CA2 (p < .05), and CA1 (p < .01) regions of the hippocampus from subjects with schizophrenia. Tritium 14-16 carbonic anhydrase 2 Homo sapiens 104-107 17239354-3 2007 RESULTS: The [3H]pirenzepine binding was decreased in the dentate gyrus (p < .05), CA3 (p < .01), CA2 (p < .05), and CA1 (p < .01) regions of the hippocampus from subjects with schizophrenia. Tritium 14-16 carbonic anhydrase 1 Homo sapiens 126-129 17229541-6 2007 Moreover, disturbed dendritic structure in the CA1 and CA3 stratum radiatum was manifested as formation of varicosities 3h after the kainic acid treatment. Tritium 120-122 carbonic anhydrase 3 Rattus norvegicus 55-58 17326770-5 2007 Down-regulation of caveolin-1 by siRNA reduced the interaction between p-gp and caveolin-1, followed by a decrease in [3H]-Taxol and [3H]-Vinblastine accumulation in RBE4 cells. Tritium 119-121 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 71-75 17326770-5 2007 Down-regulation of caveolin-1 by siRNA reduced the interaction between p-gp and caveolin-1, followed by a decrease in [3H]-Taxol and [3H]-Vinblastine accumulation in RBE4 cells. Tritium 134-136 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 71-75 17234339-3 2007 Treatment of mouse dopaminergic CATH.a cells with 2 mM methamphetamine increased PAG608 expression at 3h followed by increase in phosphorylated p53 expression. Tritium 102-104 cathepsin H Mus musculus 32-36 17364745-4 2007 It was found that 25 microM of the pan-isoform PKC inhibitor, chelerythrine, reduced [3H]D-aspartate uptake by 78%, 71%, and 68% in isolated retinas, mixed neuronal/glial cultures, and Muller cell-enriched cultures, respectively. Tritium 86-88 protein kinase C, gamma Rattus norvegicus 47-50 17510552-5 2007 METHODS: Tegaserod inhibition of SERT-mediated [3H]5-HT and NET- and DAT-mediated [3H]dopamine uptake was measured in human embryonic kidney (HEK) 293 cells stably expressing hSERT, hDAT, and hNET in comparison with untransfected control HEK293-FT cells. Tritium 83-85 solute carrier family 6 member 3 Homo sapiens 69-72 16823772-4 2006 The EGF-induced increase in [3H]-thymidine incorporation was blocked by AG 1478 (an EGF receptor tyrosine kinase antagonist), genistein, and herbimycin A (tyrosine kinase inhibitors), suggesting a role in the activation and tyrosine phosphorylation of the EGF receptor. Tritium 29-31 epidermal growth factor Gallus gallus 4-7 16823772-4 2006 The EGF-induced increase in [3H]-thymidine incorporation was blocked by AG 1478 (an EGF receptor tyrosine kinase antagonist), genistein, and herbimycin A (tyrosine kinase inhibitors), suggesting a role in the activation and tyrosine phosphorylation of the EGF receptor. Tritium 29-31 epidermal growth factor Gallus gallus 84-87 16823772-4 2006 The EGF-induced increase in [3H]-thymidine incorporation was blocked by AG 1478 (an EGF receptor tyrosine kinase antagonist), genistein, and herbimycin A (tyrosine kinase inhibitors), suggesting a role in the activation and tyrosine phosphorylation of the EGF receptor. Tritium 29-31 epidermal growth factor Gallus gallus 84-87 16823772-5 2006 In addition, the EGF-induced stimulation of [3H]-thymidine incorporation was prevented by staurosporine, H-7, or bisindolylmaleimide I (protein kinase C inhibitors), suggesting a role of PKC. Tritium 45-47 epidermal growth factor Gallus gallus 17-20 16823772-6 2006 In addition, PD 98059 (a MEK inhibitor), SB 203580 (a p38 MAPK inhibitor), and SP 600125 (a JNK inhibitor) blocked the EGF-induced stimulation of [3H]-thymidine incorporation and CDK-2/4 expression. Tritium 147-149 adapter molecule crk Gallus gallus 54-57 16823772-6 2006 In addition, PD 98059 (a MEK inhibitor), SB 203580 (a p38 MAPK inhibitor), and SP 600125 (a JNK inhibitor) blocked the EGF-induced stimulation of [3H]-thymidine incorporation and CDK-2/4 expression. Tritium 147-149 epidermal growth factor Gallus gallus 119-122 17384278-7 2006 CXCR4 was functionally coupled as demonstrated by the significant increase of DNA synthesis in meningioma cells in response to SDF1, measured by [3H]-thymidine uptake. Tritium 146-148 C-X-C motif chemokine receptor 4 Homo sapiens 0-5 16941497-5 2006 Under these reduced APP levels, we studied 3H-choline uptake in CTb and CNh cells. Tritium 43-45 chitobiase Mus musculus 64-67 16908007-3 2006 Renal mRNA levels of calnexin significantly increased 3h after an injection of Fe-NTA in rats fed a standard diet whereas those fed an alpha-tocopherol-supplemented diet showed an increase prior to injection, but after injection showed a decrease in renal calnexin mRNA levels, with unaltered protein levels. Tritium 54-56 calnexin Rattus norvegicus 21-29 16640505-7 2006 In isolated lung myofibroblasts, AT1 and AT2 receptors were expressed, and Ang II stimulated proliferation as measured by [3H]thymidine incorporation. Tritium 123-125 angiogenin Rattus norvegicus 75-78 16505160-4 2006 The majority of the KOR in the placenta and FLAG-hKOR in CHO cells, determined by [3H]diprenorphine binding and/or immunoblotting with an anti-FLAG antibody, was present in low-density fractions, coinciding with high levels of caveolin-1 and cholesterol, markers of lipid rafts, which indicated that the KOR is localized in lipid rafts. Tritium 83-85 opioid receptor kappa 1 Homo sapiens 20-23 16505160-4 2006 The majority of the KOR in the placenta and FLAG-hKOR in CHO cells, determined by [3H]diprenorphine binding and/or immunoblotting with an anti-FLAG antibody, was present in low-density fractions, coinciding with high levels of caveolin-1 and cholesterol, markers of lipid rafts, which indicated that the KOR is localized in lipid rafts. Tritium 83-85 opioid receptor kappa 1 Homo sapiens 49-53 16505160-4 2006 The majority of the KOR in the placenta and FLAG-hKOR in CHO cells, determined by [3H]diprenorphine binding and/or immunoblotting with an anti-FLAG antibody, was present in low-density fractions, coinciding with high levels of caveolin-1 and cholesterol, markers of lipid rafts, which indicated that the KOR is localized in lipid rafts. Tritium 83-85 opioid receptor kappa 1 Homo sapiens 50-53 16546225-7 2006 caused a dose-dependent inhibition of ex vivo [3H]-GR125743 binding to rat striatal 5-HT1B receptors with an ED50 of 2.83+/-0.39 mg kg(-1) p.o. Tritium 47-49 5-hydroxytryptamine receptor 1B Rattus norvegicus 84-90 16457827-9 2006 While insulin administration stimulated LPL activity of adipose tissue 3h after injection, no response was observed in red or white muscles. Tritium 71-73 lipoprotein lipase Oncorhynchus mykiss 40-43 16001112-7 2006 RESULTS: Beta2+/+ mice developed dose-dependent tolerance and upregulation of [3H]epibatidine binding sites. Tritium 79-81 hemoglobin, beta adult minor chain Mus musculus 9-14 16255717-5 2006 [3H]Octadecyl-labelled PAF was incubated with purified rat intestinal alk-SMase or recombinant human alk-SMase expressed in COS-7 cells. Tritium 1-3 PCNA clamp associated factor Rattus norvegicus 23-26 16255717-5 2006 [3H]Octadecyl-labelled PAF was incubated with purified rat intestinal alk-SMase or recombinant human alk-SMase expressed in COS-7 cells. Tritium 1-3 ectonucleotide pyrophosphatase/phosphodiesterase 7 Rattus norvegicus 70-79 16255717-5 2006 [3H]Octadecyl-labelled PAF was incubated with purified rat intestinal alk-SMase or recombinant human alk-SMase expressed in COS-7 cells. Tritium 1-3 ectonucleotide pyrophosphatase/phosphodiesterase 7 Homo sapiens 101-110 16376309-5 2006 PBR ligand binding density was assayed with the specific PBR ligand [3H]PK 11195. Tritium 69-71 translocator protein Rattus norvegicus 0-3 16376309-5 2006 PBR ligand binding density was assayed with the specific PBR ligand [3H]PK 11195. Tritium 69-71 translocator protein Rattus norvegicus 57-60 16331283-5 2006 In hPAT1 and mPAT1-expressing Xenopus laevis oocytes [3H]GABA uptake was inhibited by vigabatrin, TACA and guvacine, whereas gabapentin failed to inhibit [3H]GABA uptake. Tritium 54-56 solute carrier family 36 (proton/amino acid symporter), member 1 Mus musculus 13-18 16386712-3 2006 The translocation of PKC from the cytosol to the membrane was studied using the phorbol ester 12,13-dibutyrate ([3H]PDBu) binding in control and bilateral entorhinal cortex (EC) lesioned rats. Tritium 113-115 protein kinase C, gamma Rattus norvegicus 21-24 16386712-7 2006 In both CA1 and CA3 hippocampal subfields, odor stimulation induced an increase of [3H]PDBu binding in both control and EC-lesioned animals, the increase being potentiated only in CA1 of lesioned rats. Tritium 84-86 carbonic anhydrase 3 Rattus norvegicus 16-19 16100249-12 2006 ANG II induced a 24% increase in [3H]Leu incorporation and a 65.8% increase in cell size compared with control cardiomyocytes (P < 0.01), which was prevented by treatment with losartan, an angiotensin (AT1) receptor inhibitor, or transfection with pSi-1. Tritium 34-36 angiogenin Rattus norvegicus 0-3 16133534-9 2006 [3H]dFdC-anabolism increased 6-44 fold in SK-N-BE(2)c cells after incubation with CPEC and was paralleled by a significant increase in expression and activity of dCK. Tritium 1-3 Calcium/calmodulin-dependent protein kinase II Drosophila melanogaster 162-165 16424786-6 2006 Therefore, in this study we tested the hypothesis that P-gp modulates the uptake of aldosterone into the brain and heart by comparing the plasma and tissue distribution of [3H]-aldosterone in wild-type and P-gp-deficient [mdr1a/1b (-/-)] mice. Tritium 173-175 phosphoglycolate phosphatase Mus musculus 55-59 16298345-6 2005 [3H]-maraviroc bound with high affinity to CCR5 from macaque (K(d) = 1.36+/-0.07 nM) and human (K(d) = 0.86 +/- 0.08 nM), but was found to dissociate approximately 10-fold more quickly from macaque CCR5. Tritium 1-3 C-C motif chemokine receptor 5 Homo sapiens 43-47 16298345-6 2005 [3H]-maraviroc bound with high affinity to CCR5 from macaque (K(d) = 1.36+/-0.07 nM) and human (K(d) = 0.86 +/- 0.08 nM), but was found to dissociate approximately 10-fold more quickly from macaque CCR5. Tritium 1-3 C-C motif chemokine receptor 5 Homo sapiens 198-202 16309561-5 2005 RESULTS: [3H] Win 35,428 saturation binding assays and DAT immunoblots revealed statistically significant differences in DAT expression attributable to DAT1 genotype. Tritium 10-12 solute carrier family 6 member 3 Homo sapiens 121-124 16309561-5 2005 RESULTS: [3H] Win 35,428 saturation binding assays and DAT immunoblots revealed statistically significant differences in DAT expression attributable to DAT1 genotype. Tritium 10-12 solute carrier family 6 member 3 Homo sapiens 152-156 16320832-7 2005 EG-VEGF/PK-1 elevated [3H]-thymidine incorporation, MAPK activation and c-jun/fos mRNA expression and enhanced LEC proliferation. Tritium 23-25 prokineticin 1 Homo sapiens 0-7 16133421-4 2005 Rabbits infected with Ad/hSR-BI (adenoviral plasmids containing hSR-BI) showed a faster clearance of administered [3H]HDL cholesterol and significantly decreased apolipoprotein (apo) A-I levels when compared to control rabbits, respectively. Tritium 115-117 scavenger receptor class B member 1 Homo sapiens 25-31 16133421-4 2005 Rabbits infected with Ad/hSR-BI (adenoviral plasmids containing hSR-BI) showed a faster clearance of administered [3H]HDL cholesterol and significantly decreased apolipoprotein (apo) A-I levels when compared to control rabbits, respectively. Tritium 115-117 scavenger receptor class B member 1 Homo sapiens 64-70 16120751-4 2005 PCB 50 stimulated the release of 3H-AA from gd20 RMC in concentration- and time-dependent manners (p < 0.05). Tritium 33-35 pyruvate carboxylase Rattus norvegicus 0-3 16081420-2 2005 We found that smooth muscle (MSMC) and endothelial (MEC) cultures obtained from mouse brain microvessels convert [3H]20-HETE to 20-COOH-AA, indicating that the cerebral vasculature can produce this metabolite. Tritium 114-116 chemokine (C-C motif) ligand 28 Mus musculus 52-55 16081420-4 2005 N-Heptylformamide, a potent inhibitor of alcohol dehydrogenase (ADH), decreased the conversion of [3H]20-HETE to 20-COOH-AA by the MSMC and MEC and also by isolated mouse brain microvessels. Tritium 99-101 chemokine (C-C motif) ligand 28 Mus musculus 140-143 16026763-4 2005 Using cultured bovine tracheal smooth muscle (BTSM) cells as a model system, thrombin stimulated a marked increase in [3H]inositol phosphate ([3H]InsPs) accumulation, which was fully mimicked by a selective PAR1 activating peptide. Tritium 119-121 coagulation factor II, thrombin Bos taurus 77-85 16026763-4 2005 Using cultured bovine tracheal smooth muscle (BTSM) cells as a model system, thrombin stimulated a marked increase in [3H]inositol phosphate ([3H]InsPs) accumulation, which was fully mimicked by a selective PAR1 activating peptide. Tritium 143-145 coagulation factor II, thrombin Bos taurus 77-85 15662045-6 2005 In mutant rOCT1(L447Y/Q448E), the IC50 value for the inhibition of [3H]MPP (0.1 microM) uptake by corticosterone (24 +/- 4 microM) was significantly higher compared with the IC50 value for inhibition of [14C]TEA (10 microM) uptake (5.3 +/- 1.7 microM). Tritium 68-70 solute carrier family 22 member 1 Rattus norvegicus 10-15 15671029-5 2005 The VDR ligand binding domain bound [3H]1alpha,25(OH)2D3 as effectively as wild type VDR but did not induce vitamin D response element-mediated transcription. Tritium 37-39 vitamin D (1,25-dihydroxyvitamin D3) receptor Mus musculus 4-7 15663892-5 2005 The modulating effect of adenosine deaminase on A1R was studied by using [3H]DPCPX binding assay and an intracellular calcium assay. Tritium 74-76 adenosine deaminase Homo sapiens 25-44 15498883-6 2005 After the administration of [26,27-methyl-3H]25(OH)D3, Cyp24a1-null mice showed higher [26,27-methyl-3H]25(OH)D3 levels and no [26,27-methyl-3H]24,25(OH)2D3 formation, whereas heterozygotic mice showed significant [26,27-methyl-3H]24,25(OH)2D3 production. Tritium 42-44 cytochrome P450, family 24, subfamily a, polypeptide 1 Mus musculus 55-62 15498883-6 2005 After the administration of [26,27-methyl-3H]25(OH)D3, Cyp24a1-null mice showed higher [26,27-methyl-3H]25(OH)D3 levels and no [26,27-methyl-3H]24,25(OH)2D3 formation, whereas heterozygotic mice showed significant [26,27-methyl-3H]24,25(OH)2D3 production. Tritium 101-103 cytochrome P450, family 24, subfamily a, polypeptide 1 Mus musculus 55-62 15498883-6 2005 After the administration of [26,27-methyl-3H]25(OH)D3, Cyp24a1-null mice showed higher [26,27-methyl-3H]25(OH)D3 levels and no [26,27-methyl-3H]24,25(OH)2D3 formation, whereas heterozygotic mice showed significant [26,27-methyl-3H]24,25(OH)2D3 production. Tritium 101-103 cytochrome P450, family 24, subfamily a, polypeptide 1 Mus musculus 55-62 15498883-6 2005 After the administration of [26,27-methyl-3H]25(OH)D3, Cyp24a1-null mice showed higher [26,27-methyl-3H]25(OH)D3 levels and no [26,27-methyl-3H]24,25(OH)2D3 formation, whereas heterozygotic mice showed significant [26,27-methyl-3H]24,25(OH)2D3 production. Tritium 101-103 cytochrome P450, family 24, subfamily a, polypeptide 1 Mus musculus 55-62 15528320-4 2005 3H-DPDPE uptake in suspended Wistar rat hepatocytes was primarily (>95%) due to temperature-dependent transport mechanisms; similar results were obtained in suspended hepatocytes from Mrp2-deficient (TR-) rats. Tritium 0-2 ATP binding cassette subfamily C member 2 Rattus norvegicus 187-191 15576844-5 2005 The plasma half-life of [3H]TG-VLDL-mimicking particles was 2-fold increased in APOC1 mice, suggesting that apoC-I reduces the lipolytic conversion of VLDL. Tritium 25-27 apolipoprotein C-I Mus musculus 80-85 15576844-5 2005 The plasma half-life of [3H]TG-VLDL-mimicking particles was 2-fold increased in APOC1 mice, suggesting that apoC-I reduces the lipolytic conversion of VLDL. Tritium 25-27 apolipoprotein C-I Mus musculus 108-114 15576844-6 2005 Although total postheparin plasma LPL activity was not lower in APOC1 mice compared with controls, apoC-I was able to dose-dependently inhibit the LPL-mediated lipolysis of [3H]TG-VLDL-mimicking particles in vitro with a 60% efficiency compared with the main endogenous LPL inhibitor apoC-III. Tritium 174-176 apolipoprotein C-I Mus musculus 99-105 15576844-6 2005 Although total postheparin plasma LPL activity was not lower in APOC1 mice compared with controls, apoC-I was able to dose-dependently inhibit the LPL-mediated lipolysis of [3H]TG-VLDL-mimicking particles in vitro with a 60% efficiency compared with the main endogenous LPL inhibitor apoC-III. Tritium 174-176 lipoprotein lipase Mus musculus 147-150 15576844-6 2005 Although total postheparin plasma LPL activity was not lower in APOC1 mice compared with controls, apoC-I was able to dose-dependently inhibit the LPL-mediated lipolysis of [3H]TG-VLDL-mimicking particles in vitro with a 60% efficiency compared with the main endogenous LPL inhibitor apoC-III. Tritium 174-176 lipoprotein lipase Mus musculus 147-150 15895818-6 2005 Activation of protein kinase C (PKC) by 100 nM PDBu, increased [3H]MK-801 binding in cortex, caudate-putamen, thalamus, central gray, and cerebellum of HAS rats but activation of PKC did not influence [3H]MK-801 binding in LAS rats. Tritium 64-66 protein kinase C, gamma Rattus norvegicus 14-30 15895818-6 2005 Activation of protein kinase C (PKC) by 100 nM PDBu, increased [3H]MK-801 binding in cortex, caudate-putamen, thalamus, central gray, and cerebellum of HAS rats but activation of PKC did not influence [3H]MK-801 binding in LAS rats. Tritium 64-66 protein kinase C, gamma Rattus norvegicus 32-35 15895818-6 2005 Activation of protein kinase C (PKC) by 100 nM PDBu, increased [3H]MK-801 binding in cortex, caudate-putamen, thalamus, central gray, and cerebellum of HAS rats but activation of PKC did not influence [3H]MK-801 binding in LAS rats. Tritium 202-204 protein kinase C, gamma Rattus norvegicus 14-30 15895818-6 2005 Activation of protein kinase C (PKC) by 100 nM PDBu, increased [3H]MK-801 binding in cortex, caudate-putamen, thalamus, central gray, and cerebellum of HAS rats but activation of PKC did not influence [3H]MK-801 binding in LAS rats. Tritium 202-204 protein kinase C, gamma Rattus norvegicus 32-35 15895818-7 2005 These activation of PKC differentiates between [3H]MK-801 binding of HAS and LAS rats in frontal cortex (layer II-IV and cingulate), caudate-putamen, and ventral lateral thalamic nuclei. Tritium 48-50 protein kinase C, gamma Rattus norvegicus 20-23 15771234-6 2005 RESULTS: Rifampin inhibited the P-gp-mediated efflux of [3H]-verapamil, regardless of delivery route (Imax = 62 +/- 6%). Tritium 57-59 phosphoglycolate phosphatase Mus musculus 32-36 15450942-9 2004 Binding studies using the selective cannabinoid CB1 receptor antagonist, [3H]SR141716A, indicated the presence of the specific binding site. Tritium 74-76 cannabinoid receptor 1 Rattus norvegicus 48-51 15475457-8 2004 Thymidylate synthase activity was determined in vitro in tumors from untreated mice by [3H] release from [3H]dUMP. Tritium 88-90 thymidylate synthase Mus musculus 0-20 15475457-8 2004 Thymidylate synthase activity was determined in vitro in tumors from untreated mice by [3H] release from [3H]dUMP. Tritium 106-108 thymidylate synthase Mus musculus 0-20 15234106-2 2004 We aimed at investigating the effect of nicotinic receptor (nAChR) activation on [3H]-acetylcholine ([3H]-ACh) release from longitudinal muscle-myenteric plexus of the rat ileum and to evaluate whether this could be modulated by adenosine, an endogenous neuromodulator typically operating changes in intracellular cyclic AMP. Tritium 82-84 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 60-65 15234106-2 2004 We aimed at investigating the effect of nicotinic receptor (nAChR) activation on [3H]-acetylcholine ([3H]-ACh) release from longitudinal muscle-myenteric plexus of the rat ileum and to evaluate whether this could be modulated by adenosine, an endogenous neuromodulator typically operating changes in intracellular cyclic AMP. Tritium 102-104 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 60-65 15234106-3 2004 The nAChR agonist, 1,1-dimethyl-4-phenylpiperazinium (DMPP, 1-30 microM, 3 min) increased [3H]-ACh release in a concentration-dependent manner. Tritium 91-93 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 4-9 15234106-11 2004 The results indicate that nAChR-induced [3H]-ACh release is triggered by the influx of Ca2+, independent of voltage-sensitive calcium channels, presumably directly through nAChRs located on myenteric axon terminals. Tritium 41-43 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 26-31 15234106-12 2004 It was also shown that endogenous adenosine, activating A(2A) receptors coupled to the adenylate cyclase/cyclic AMP transducing system, is tonically downregulating this nAChR-mediated control of [3H]-ACh release. Tritium 196-198 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 169-174 15339391-7 2004 U0126, an MEK1/2 inhibitor, increased CT-1-induced the phosphorylation of STAT3 in a dose-dependent manner and, consistently, augmented CT-1-induced increase in [3H]leucine incorporation and cellular protein-to-DNA ratio by 17.6 % and 16.3 %, respectively. Tritium 162-164 cardiotrophin 1 Rattus norvegicus 136-140 15102929-5 2004 High concentrations of lobeline induced a statistically significant release of [3H]DA from HEK-hDAT-hVMAT2 cells, but only in the absence of DHTB, suggesting an hVMAT2-mediated effect. Tritium 80-82 solute carrier family 6 member 3 Homo sapiens 95-99 15318347-7 2004 [3H]ryanodine-binding analysis suggests that long-lasting changes in RyR1 conformation persists in vitro after the triggering event. Tritium 1-3 ryanodine receptor 1 Equus caballus 69-73 15268905-8 2004 The RFP expressing control cells and the parental TE85 cells each showed an approximately twofold increase (P < 0.01) in [3H]thymidine incorporation in response to the shear stress. Tritium 125-127 tripartite motif containing 27 Homo sapiens 4-7 15260125-3 2004 This effect of 5-CT on [3H]5-HT overflow was antagonized by the 5-HT7 receptor antagonist SB-258719 (10 microM) and the 5-HT(1B/1D) antagonist SB-216641 (1 microM), the IC50 values for 5-CT in the presence of SB-258719 and SB-216641 were 94.23 +/- 4.84 and 47.81 +/- 4.66 nM. Tritium 24-26 5-hydroxytryptamine receptor 1B Rattus norvegicus 120-127 15234254-5 2004 In contrast, BDNF (3-100 ng/ml) inhibited K+-evoked [3H]GABA release from hippocampal synaptosomes. Tritium 53-55 brain-derived neurotrophic factor Rattus norvegicus 13-17 14726302-5 2004 The activity of P450(arom) was demonstrated by the stereospecific loss of the tritium atom of [1beta-(3)H]androstenedione. Tritium 78-85 PARP1 binding protein Rattus norvegicus 16-26 15247401-5 2004 By contrast, complementation by AtOPT6 restored growth of the hgt1 yeast mutant on a medium containing reduced (GSH) or oxidized glutathione as the sole sulfur source and induced uptake of [3H]GSH, whereas complementation by AtOPT7 did not. Tritium 190-192 oligopeptide transporter 1 Arabidopsis thaliana 32-38 14736843-4 2004 RT-induced inhibition of [3H]NE release was prevented by synaptosomal entrapment of polyclonal antibodies directed against KCNQ2 subunits, an effect that was abolished by antibody preabsorption with the KCNQ2 immunizing peptide; antibodies against KCNQ3 subunits were ineffective. Tritium 26-28 potassium voltage-gated channel subfamily Q member 3 Rattus norvegicus 248-253 13129851-4 2004 [3H]PAH and 3H-labeled estrone sulfate ([3H]ES) were used as specific substrates for rbOAT1 and rbOAT3, respectively. Tritium 1-3 solute carrier family 22 member 8 Oryctolagus cuniculus 96-102 13129851-4 2004 [3H]PAH and 3H-labeled estrone sulfate ([3H]ES) were used as specific substrates for rbOAT1 and rbOAT3, respectively. Tritium 12-14 solute carrier family 22 member 8 Oryctolagus cuniculus 96-102 13129851-4 2004 [3H]PAH and 3H-labeled estrone sulfate ([3H]ES) were used as specific substrates for rbOAT1 and rbOAT3, respectively. Tritium 12-14 solute carrier family 22 member 8 Oryctolagus cuniculus 96-102 14675564-3 2004 Evaluation of kinetic parameters of PBR was performed using [3H]PK11195, as specific radioligand compared with 15 healthy volunteers. Tritium 61-63 translocator protein Homo sapiens 36-39 14690536-7 2004 Reflecting the decrease in GLT-1 protein, [3H]d-aspartate uptake was reduced in cultures expressing hSOD1(G93A) or hSOD1wt. Tritium 43-45 solute carrier family 1 (glial high affinity glutamate transporter), member 2 Mus musculus 27-32 15262334-3 2004 Utilizing [3H]BC, we have identified several proteins to which BC binds with high affinity (e.g. the chaperone member glucose regulated protein 78, the enzyme carboxylesterase, the cytochrome P450 2E1, the enzyme monoamine oxidase B and a small G-protein of the Rho subfamily). Tritium 11-13 monoamine oxidase B Homo sapiens 213-232 15262334-4 2004 In the present study we isolated a protein from bovine brain to which [3H]BC binds with high affinity and identified it being the enzyme triosephosphate isomerase (TPI; EC 5.3.1.1.). Tritium 71-73 triosephosphate isomerase 1 Bos taurus 137-162 15262334-4 2004 In the present study we isolated a protein from bovine brain to which [3H]BC binds with high affinity and identified it being the enzyme triosephosphate isomerase (TPI; EC 5.3.1.1.). Tritium 71-73 triosephosphate isomerase 1 Bos taurus 164-167 12941963-2 2003 In rat visual cortex, [3H]SR-95531 binding assays revealed that brain-derived neurotrophic factor (BDNF), one of the neurotrophins, induced a rapid increase in the total number of cell surface GABAA receptors, through the activation of Trk B receptor tyrosine kinases. Tritium 23-25 brain-derived neurotrophic factor Rattus norvegicus 64-97 12941963-2 2003 In rat visual cortex, [3H]SR-95531 binding assays revealed that brain-derived neurotrophic factor (BDNF), one of the neurotrophins, induced a rapid increase in the total number of cell surface GABAA receptors, through the activation of Trk B receptor tyrosine kinases. Tritium 23-25 brain-derived neurotrophic factor Rattus norvegicus 99-103 12915407-4 2003 Although the lack of a functional PTL delayed fat absorption during the initial hour of feeding a bolus load of olive oil containing [3H]triolein and [14C]cholesterol, the rate of [3H]triolein absorption was similar between PTL+/+ and PTL-/- mice after the initial 1-h period. Tritium 134-136 pancreatic lipase Mus musculus 34-37 12915407-4 2003 Although the lack of a functional PTL delayed fat absorption during the initial hour of feeding a bolus load of olive oil containing [3H]triolein and [14C]cholesterol, the rate of [3H]triolein absorption was similar between PTL+/+ and PTL-/- mice after the initial 1-h period. Tritium 181-183 pancreatic lipase Mus musculus 34-37 12893836-6 2003 We then characterized rMRP1-mediated transport properties in MLS-9 cells using [3H]vincristine, a known MRP1 substrate. Tritium 80-82 ATP binding cassette subfamily C member 1 Rattus norvegicus 22-27 12893836-6 2003 We then characterized rMRP1-mediated transport properties in MLS-9 cells using [3H]vincristine, a known MRP1 substrate. Tritium 80-82 ATP binding cassette subfamily C member 1 Rattus norvegicus 23-27 12637261-6 2003 The fractional catabolic rates of both 125I-labeled tyraminecellobiose (TC)-HDL and [3H]cholesteryl ether increased significantly in the sPLA2-transgenic mice after induction of the APR (0.18 +/- 0.01 vs. 0.21 +/- 0.01 pool/h, P < 0.05, and 0.31 +/- 0.02 vs. 0.42 +/- 0.05 pool/h, P < 0.05, respectively) but remained unchanged in the wild-type mice (0.10 +/- 0.01 vs. 0.22 +/- 0.02 pool/h, respectively). Tritium 85-87 phospholipase A2, group IIA (platelets, synovial fluid) Mus musculus 137-142 12740863-2 2003 We investigated the ability of subtype-specific nAChR antagonists to depress nerve-evoked contractions and [(3)H]-acetylcholine ([(3)H]-ACh) release. Tritium 107-113 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 48-53 12859460-11 2003 mMCP-2 bound [3H]-labelled DFP, indicating the presence of an active serine proteinase catalytic site, but showed little evidence of chymotryptic activity. Tritium 14-16 mast cell protease 2 Mus musculus 0-6 12808449-6 2003 Moreover, SSeCKS-CM increased tight junction proteins in endothelial cells, consequently decreasing [3H]sucrose permeability. Tritium 101-103 A-kinase anchoring protein 12 Homo sapiens 10-16 12727318-3 2003 Saturation binding studies using [3H]mazindol showed a high affinity DAT binding site with K(D) and B(max) values of 12.32+/-1.67 nM and 82.7+/-9.74 fmol/mg protein, respectively. Tritium 34-36 solute carrier family 6 member 3 Homo sapiens 69-72 12934358-7 2003 The ginsenosides, ginsenoside Rb1, and ginsenoside Rg1 promoted the [3H] choline incorporation. Tritium 69-71 RB transcriptional corepressor 1 Rattus norvegicus 30-33 12675917-2 2003 The external Ca2+-dependent K+-induced [3H]catecholamine overflows were almost totally abolished by botulinum toxin C1 (BoNT/C1), which hydrolyses syntaxin and SNAP-25, or by botulinum toxin E (BoNT/E), selective for SNAP-25. Tritium 40-42 synaptosome associated protein 25 Rattus norvegicus 160-167 12675917-2 2003 The external Ca2+-dependent K+-induced [3H]catecholamine overflows were almost totally abolished by botulinum toxin C1 (BoNT/C1), which hydrolyses syntaxin and SNAP-25, or by botulinum toxin E (BoNT/E), selective for SNAP-25. Tritium 40-42 synaptosome associated protein 25 Rattus norvegicus 217-224 12640721-8 2003 3H-inulin flux studies confirmed IFN-alpha-induced increases in paracellular permeability of cholangiocytes (P < 0.001). Tritium 0-2 interferon alpha Mus musculus 33-42 15618750-0 2003 Blood-brain permeability of [3H]-(3-methyl-His2)thyrotropin-releasing hormone (MeTRH) in mice: effects of TRH and its analogues. Tritium 29-31 thyrotropin releasing hormone Mus musculus 48-77 15618750-0 2003 Blood-brain permeability of [3H]-(3-methyl-His2)thyrotropin-releasing hormone (MeTRH) in mice: effects of TRH and its analogues. Tritium 29-31 thyrotropin releasing hormone Mus musculus 81-84 12505063-6 2002 Continuous infusion of Ang II induced a rapid, AT(1)-mediated increase of apoptotic cardiomyocyte nuclei (from 14+/-9 to 188+/-35 TdT-labeled nuclei/10(6) after 3h, P<0.005) and of activated caspase-3, that normalized after 24h. Tritium 161-163 angiogenin Rattus norvegicus 23-26 12793746-4 2002 After landing, we labeled space-radiation-induced DNA strand breaks by enzymatic incorporation of [3H]-dATP with terminal deoxyribo-nucleotidyl transferase (TdT). Tritium 99-101 DNA nucleotidylexotransferase Homo sapiens 113-155 12468277-12 2002 The Q317X mutation deletes 110 amino acids of the ligand-binding domain of the VDR and results in the loss of [3H]1,25(OH)(2)D(3) binding and target gene transactivation. Tritium 111-113 vitamin D receptor Homo sapiens 79-82 12441365-6 2002 TB was a high affinity ligand for the androgen receptor (AR), with an IC(50) of about 4 nM in rat ventral prostate cytosol and about 33 nM in cells transfected with the human AR when competed with 1 nM [3H]R1881. Tritium 203-205 androgen receptor Rattus norvegicus 38-55 12441365-6 2002 TB was a high affinity ligand for the androgen receptor (AR), with an IC(50) of about 4 nM in rat ventral prostate cytosol and about 33 nM in cells transfected with the human AR when competed with 1 nM [3H]R1881. Tritium 203-205 androgen receptor Rattus norvegicus 57-59 12490138-5 2002 Sucrose density gradient analysis of cytosolic AHR radioligand binding using a saturating concentration of [3H]TCDD showed that ligand binding was reduced in hypx rats to 31% of sham levels. Tritium 108-110 aryl hydrocarbon receptor Rattus norvegicus 47-50 12433844-5 2002 With [3H]-thymidine incorporation and immunoblots, we found that ET-1-induced cell proliferation was parallel to a significant increase in the endogenous expression of ClC-3 protein. Tritium 6-8 chloride voltage-gated channel 3 Rattus norvegicus 168-173 12491787-8 2002 ET-1 accelerated the [3H]-thymidine incorporation by cultured vascular smooth muscle cells and, on the other hand, reduced TUNEL-positive cells, which was caused by the serum deprivation. Tritium 22-24 endothelin-1 Oryctolagus cuniculus 0-4 12542050-5 2002 RESULTS: ISO group as compared with control group, myocardial taurine uptake markedly reduced, taurine release obviously increased; Bmax value of [3H]-taurine binding on cardiac sarcolemma reduced by 42% (P<0.05); TAUT and CSD mRNA levels decreased by 40% and 38% (P<0.05), respectively. Tritium 147-149 cysteine sulfinic acid decarboxylase Rattus norvegicus 226-229 12351745-2 2002 In addition to this effect, this study demonstrates that MPD increases vesicular [3H]DA uptake and binding of the vesicular monoamine transporter-2 (VMAT-2) ligand dihydrotetrabenazine (DHTBZ) in a dose- and time-dependent manner in purified striatal vesicles prepared from treated rats. Tritium 82-84 solute carrier family 18 member A2 Rattus norvegicus 149-155 12237339-8 2002 Conversely, efflux of [(3)H]PAH from Oat1-expressing oocytes was trans-stimulated by glutarate, PAH, NAC, DMPS, MeHg-NAC, MeHg-DMPS, and a mercapturic acid, indicating that these are transported solutes. Tritium 22-27 solute carrier family 22 member 6 Rattus norvegicus 37-41 12127559-3 2002 Abundant expression of IGF-I, IGF-II and IGF-IR was demonstrated in resting cells and exogenous IGF-I and IGF-II increased 3H-thymidine incorporation in a dose dependent manner. Tritium 123-125 insulin like growth factor 1 receptor Homo sapiens 41-47 12177179-7 2002 Tritium-labeled AC delivered to intact CHO cells as a cyclodextrin complex was shown to photoaffinity label several discrete polypeptides, including caveolin-1. Tritium 0-7 caveolin-1 Cricetulus griseus 149-159 12060594-4 2002 n-Tetraalkylammonium compounds showed similar affinities for the two homologs, with IC50 values for inhibition of OCT1- and OCT2-mediated [3H]TEA transport, respectively, of 4,538 and 1,395 microM for tetramethylammonium, 88.5 and 3.9 microM for tetrapropylammonium, 13.9 and 5.3 microM for tetrabutylammonium, and 8.8 and 7.6 microM for tetrapentylammonium. Tritium 139-141 solute carrier family 22 member 1 Oryctolagus cuniculus 114-118 11997255-6 2002 Enzymatic conversion of [3H]KG to Glu by glutamic dehydrogenase (GDH) or of [3H]OA to malate by malic dehydrogenase (MDH) "protects" the label from release by base, showing that base-induced isotope release is from keto acid rather than a result of release during the exchange process. Tritium 25-27 malate dehydrogenase 2 Homo sapiens 96-115 11997255-6 2002 Enzymatic conversion of [3H]KG to Glu by glutamic dehydrogenase (GDH) or of [3H]OA to malate by malic dehydrogenase (MDH) "protects" the label from release by base, showing that base-induced isotope release is from keto acid rather than a result of release during the exchange process. Tritium 25-27 malate dehydrogenase 2 Homo sapiens 117-120 11997255-6 2002 Enzymatic conversion of [3H]KG to Glu by glutamic dehydrogenase (GDH) or of [3H]OA to malate by malic dehydrogenase (MDH) "protects" the label from release by base, showing that base-induced isotope release is from keto acid rather than a result of release during the exchange process. Tritium 77-79 malate dehydrogenase 2 Homo sapiens 96-115 11997255-6 2002 Enzymatic conversion of [3H]KG to Glu by glutamic dehydrogenase (GDH) or of [3H]OA to malate by malic dehydrogenase (MDH) "protects" the label from release by base, showing that base-induced isotope release is from keto acid rather than a result of release during the exchange process. Tritium 77-79 malate dehydrogenase 2 Homo sapiens 117-120 12053014-0 2002 [3H]muscimol binding to gamma-aminobutyric acid(A) receptors is upregulated in CA1 neurons of the gerbil hippocampus in the ischemia-tolerant state. Tritium 0-4 carbonic anhydrase 1 Homo sapiens 79-82 12053014-7 2002 [3H]Muscimol binding to GABA(A) receptors in CA1 neurons was transiently but significantly increased after preconditioning but not after global ischemia with consecutive neuronal death. Tritium 1-3 carbonic anhydrase 1 Homo sapiens 45-48 12064476-11 2002 In keeping with the increase in DAT function, [3H]WIN 35,428 binding was increased in the cocaine users, but not in the victims of excited delirium. Tritium 47-49 solute carrier family 6 member 3 Homo sapiens 32-35 11888550-0 2002 Specific labelling of serotonin 5-HT(1B) receptors in rat frontal cortex with the novel, phenylpiperazine derivative, [3H]GR125,743. Tritium 119-121 5-hydroxytryptamine receptor 1B Rattus norvegicus 32-39 11888550-3 2002 Inasmuch as [3H]GR125,743 specifically labels cloned, human and native guinea pig 5-HT(1B) receptors and has been employed for characterization of cerebral 5-HT(1B) receptor in the latter species [Eur. Tritium 13-15 5-hydroxytryptamine receptor 1B Rattus norvegicus 82-89 21959764-0 2002 Measurement of glutamate carboxypeptidase II (NAALADase) enzyme activity by the hydrolysis of [3H]-N-acetylaspartylglutamate (NAAG). Tritium 95-98 folate hydrolase 1B (pseudogene) Homo sapiens 46-55 12658749-1 2002 In order to investigate the effect of TGF beta 1 gene transfer on the biological characteristics, the effects of gene transfer and supernatant of transfected osteoblasts on the proliferation and ALP activity of osteoblasts were detected by 3H-TdR and MTT. Tritium 240-242 PDZ and LIM domain 3 Rattus norvegicus 195-198 11833419-1 2001 The present study was undertaken to investigate the effects of angiotensin-(1-7)[Ang-(1-7)] on the proliferation and expression of protein kinase C (PKC) and extracellular regulated protein kinases (ERK1/2) of vascular smooth muscle cells (VSMCs) by techniques of Western blot, 3H-thymidine (3H-TdR) and 3H-leucine (3H-Leu) incorporation. Tritium 278-280 angiogenin Rattus norvegicus 81-84 11833419-1 2001 The present study was undertaken to investigate the effects of angiotensin-(1-7)[Ang-(1-7)] on the proliferation and expression of protein kinase C (PKC) and extracellular regulated protein kinases (ERK1/2) of vascular smooth muscle cells (VSMCs) by techniques of Western blot, 3H-thymidine (3H-TdR) and 3H-leucine (3H-Leu) incorporation. Tritium 278-280 protein kinase C, gamma Rattus norvegicus 131-147 11833419-1 2001 The present study was undertaken to investigate the effects of angiotensin-(1-7)[Ang-(1-7)] on the proliferation and expression of protein kinase C (PKC) and extracellular regulated protein kinases (ERK1/2) of vascular smooth muscle cells (VSMCs) by techniques of Western blot, 3H-thymidine (3H-TdR) and 3H-leucine (3H-Leu) incorporation. Tritium 278-280 protein kinase C, gamma Rattus norvegicus 149-152 11408552-12 2001 The products of phospholipid hydrolysis produced by cPLA2, lysophosphatidylcholine but not arachidonic acid, increased [3H]thymidine incorporation in U-937 cells. Tritium 120-122 phospholipase A2 group IVA Homo sapiens 52-57 11430910-3 2001 Of the P-glycoprotein modulators tested, rhodamine123, verapamil and daunomycin concentration-dependently inhibited EMT-mediated uptake of [3H]1-methyl-4-phenylpyridinium ([3H]MPP(+)). Tritium 140-142 solute carrier family 22 member 3 Homo sapiens 116-119 11503967-7 2001 Furthermore, MT2 cells treated with Gfi-1 antisense oligonucleotide had reduced [3H]thymidine uptake compared with MT2 cells treated with Gfi-1 sense oligonucleotide. Tritium 81-83 growth factor independent 1 transcriptional repressor Homo sapiens 36-41 11697207-5 2001 The ability of several sedating and non-sedating antihistamines to affect the uptake of the Pgp model substrate [3H]-colchicine was examined using the immortalised rat brain endothelial cell line, RBE4, an established in vitro model of the BBB expressing Pgp. Tritium 113-115 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 92-95 11282490-5 2001 Uptake of [3H]-testosterone in the regenerating liver was significantly higher during the first 6 h. 5alpha-reductase I and DHT were increased in parallel to the hepatocyte proliferation which was assessed by proliferating cell nuclear antigen (PCNA) labeling index. Tritium 11-13 proliferating cell nuclear antigen Rattus norvegicus 209-243 11282490-5 2001 Uptake of [3H]-testosterone in the regenerating liver was significantly higher during the first 6 h. 5alpha-reductase I and DHT were increased in parallel to the hepatocyte proliferation which was assessed by proliferating cell nuclear antigen (PCNA) labeling index. Tritium 11-13 proliferating cell nuclear antigen Rattus norvegicus 245-249 11420069-8 2001 [3H]SCH 23390 (DA D(1)) binding sites were downregulated in the nucleus accumbens and striatum and were upregulated in the prefrontal cortex of caffeine-treated vs. control rats; however, the affinity of [3H]SCH 23390 for these binding sites was unaltered. Tritium 1-3 defender against cell death 1 Rattus norvegicus 15-22 11420069-8 2001 [3H]SCH 23390 (DA D(1)) binding sites were downregulated in the nucleus accumbens and striatum and were upregulated in the prefrontal cortex of caffeine-treated vs. control rats; however, the affinity of [3H]SCH 23390 for these binding sites was unaltered. Tritium 205-207 defender against cell death 1 Rattus norvegicus 15-22 11341508-0 2001 Studies on the binding of 3H-SR121566, an inhibitor of Gp IIb-IIIa activation. Tritium 26-28 integrin subunit alpha 2b Homo sapiens 55-61 11341508-2 2001 3H-SR121566 bound with nanomolar affinity (KD ranging from 45 to 72 nM) to Gp IIb-IIIa expressing cells only. Tritium 0-2 integrin subunit alpha 2b Homo sapiens 75-81 11248200-4 2001 In addition, ATP-dependent uptake of [(3)H]TLC-S (K(m)=3.9 microM) and [(3)H]taurocholate (TC) (K(m)=7.5 microM) was also observed in Mrp2- and Bsep-expressing Sf9 membrane vesicles, respectively. Tritium 38-42 ATP binding cassette subfamily C member 2 Rattus norvegicus 134-138 11234755-2 2001 This article is a report on the effects of BAL and other dithiols, DMSA (meso-2,3-dimercaptosuccinic acid) and DMPS (2,3-dimercaptopropane-1-sulfonic acid), on [3H]glutamate release and uptake by rat brain synaptosomes and [3H]glutamate uptake by synaptic vesicles. Tritium 161-163 solute carrier family 27 member 5 Rattus norvegicus 43-46 11282455-2 2001 Pretreatment with dexamethasone, prednisolone and corticosterone for 60 min decreased insulin-induced [3H] 2-deoxyglucose (DOG) uptake in isolated rat adipocytes. Tritium 103-105 insulin Canis lupus familiaris 86-93 11157849-8 2001 CD28-deficient and CD7/CD28-deficient thymocytes had decreased [3H]thymidine incorporation responses to concanavalin A (Con A) stimulation compared to control mice (P < or = 0.01 and P < or = 0.05 respectively). Tritium 64-66 CD7 antigen Mus musculus 19-22 11212278-8 2001 Accumulation and efflux studies with the P-gp substrates, [3H]daunorubicin and rhodamine 123, demonstrated that XR9576 inhibited P-gp-mediated drug efflux. Tritium 59-61 phosphoglycolate phosphatase Mus musculus 41-45 11212278-8 2001 Accumulation and efflux studies with the P-gp substrates, [3H]daunorubicin and rhodamine 123, demonstrated that XR9576 inhibited P-gp-mediated drug efflux. Tritium 59-61 phosphoglycolate phosphatase Mus musculus 129-133 11301212-1 2001 We examined the effects of systemic administration of a GABA(B) receptor agonist, baclofen, or antagonist, phaclofen, on the expression of c-Fos protein induced 3h after electrical stimulation of the trigeminal ganglion at low (0.1 mA) or high intensities (1.0 mA) in the urethane-anesthetized rat. Tritium 161-163 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 139-144 11785909-2 2001 on PACAP-stimulated cyclic AMP formation in [3H]adenine-prelabeled slices of the hypothalamus and cerebral cortex. Tritium 45-47 adenylate cyclase activating polypeptide 1 Gallus gallus 3-8 11108825-3 2000 [3H]L-771,688 binding studies at the cloned human alpha1A-adrenoceptors and in rat tissues indicated that specific [3H]L-771,688 binding was saturable and of high affinity (Kd=43-90 pM) and represented binding to the pharmacologically relevant alpha1A-adrenoceptors. Tritium 116-118 calcium voltage-gated channel subunit alpha1 A Homo sapiens 50-57 11108825-3 2000 [3H]L-771,688 binding studies at the cloned human alpha1A-adrenoceptors and in rat tissues indicated that specific [3H]L-771,688 binding was saturable and of high affinity (Kd=43-90 pM) and represented binding to the pharmacologically relevant alpha1A-adrenoceptors. Tritium 116-118 calcium voltage-gated channel subunit alpha1 A Homo sapiens 244-251 10973601-7 2000 Peripheral-type benzodiazepine receptor agonists, however, did potentiate [3H]phenytoin binding, and a specific receptor antagonist (PK11195) attenuated the potentiation seen with diazepam. Tritium 75-77 translocator protein Rattus norvegicus 0-39 10974231-7 2000 Additionally, examples from Class I GPIIb/IIIa antagonists such as (3)H-active form of Roxifiban showed no significant changes in its platelet binding affinity in response to change in [Ca(++)]. Tritium 67-71 integrin subunit alpha 2b Homo sapiens 36-41 11019978-2 2000 Factor Xa elicited a concentration-dependent increase in [3H]thymidine incorporation. Tritium 58-60 coagulation factor X Homo sapiens 0-9 10952156-6 2000 Cultured cardiac myocytes treated with Ang II and infected with either control or recombinant adenovirus indicated that expression of p21 and p16 inhibited Ang II-induced cardiac myocyte hypertrophy, [3H]leucine incorporation into total cellular proteins, and skeletal alpha-actin (SK-A) and atrial natriuretic peptide (ANP) mRNA accumulation. Tritium 201-203 KRAS proto-oncogene, GTPase Rattus norvegicus 134-137 10952156-6 2000 Cultured cardiac myocytes treated with Ang II and infected with either control or recombinant adenovirus indicated that expression of p21 and p16 inhibited Ang II-induced cardiac myocyte hypertrophy, [3H]leucine incorporation into total cellular proteins, and skeletal alpha-actin (SK-A) and atrial natriuretic peptide (ANP) mRNA accumulation. Tritium 201-203 cyclin-dependent kinase inhibitor 2A Rattus norvegicus 142-145 10910289-8 2000 Proliferative activity and production of interferon (IFN)-gamma, interleukin (IL)-4, and IL-10 by naive C3H (H2k, IEk) T cells in response to DC stimulation was assessed by [3H]thymidine incorporation, and by multicolor flow cytometric analysis, respectively, after 3-day mixed leukocyte reactions. Tritium 105-107 interleukin 10 Homo sapiens 89-94 10826405-6 2000 Based on radioligand-binding studies using [3H]-RX821002 and selective competitors, a raise in alpha2-adrenoceptor density that was solely due to an increased alpha2B-adrenoceptor subtype density was detected in the kidney of the cafeteria-fed rat. Tritium 44-46 adrenoceptor alpha 2B Rattus norvegicus 159-179 10769184-0 2000 A novel 4 S [3H]beta-naphthoflavone-binding protein in liver cytosol of female Sprague-Dawley rats treated with aryl hydrocarbon receptor agonists. Tritium 13-15 aryl hydrocarbon receptor Rattus norvegicus 112-137 10800963-5 2000 After incubation with the isothiocyanato analogues or UV irradiation of the azido analogues, the 3-substituted aryl pyrazoles formed covalent bonds with the CB1 receptor as evidenced by the loss of specific binding of [3H]CP55940. Tritium 219-221 cannabinoid receptor 1 Rattus norvegicus 157-160 10733008-2 2000 Binding site densities for the PTBR ligand [3H-PK11195] are increased in autopsied brain tissue from PSE patients as well as in the brains of animals with experimental chronic liver failure. Tritium 44-46 translocator protein Homo sapiens 31-35 10763851-4 2000 Draflazine and a series of 15 chemically related compounds were compared for their abilities to: (a) inhibit the binding of [3H]NBMPR to the es/ENT1 transporter in mouse Ehrlich cell and human erythrocyte membranes, and (b) inhibit the es/ENT1 and ei/ENT2 transporter-mediated uptake of [3H]uridine in Ehrlich cells. Tritium 125-127 solute carrier family 29 (nucleoside transporters), member 1 Mus musculus 144-148 10686583-1 2000 [(3)H]-Arachidonic acid-labelled rat T lymphocytes released radioactivity extracellularly when stimulated by the calcium ionophore A23187 or by monoclonal antibodies to some cell surface structures (CD2, CD5, CD11a, CD18, CD54, T-cell receptor) but not to others (CD49d, CD62L); release was greater with the calcium ionophore. Tritium 1-5 Cd5 molecule Rattus norvegicus 204-207 10686583-1 2000 [(3)H]-Arachidonic acid-labelled rat T lymphocytes released radioactivity extracellularly when stimulated by the calcium ionophore A23187 or by monoclonal antibodies to some cell surface structures (CD2, CD5, CD11a, CD18, CD54, T-cell receptor) but not to others (CD49d, CD62L); release was greater with the calcium ionophore. Tritium 1-5 intercellular adhesion molecule 1 Homo sapiens 222-226 10685879-6 2000 The inhibitory effect on [3H]DA uptake was reversible and not dependent on pH, as observed in HeLa cells stably expressing DAT. Tritium 26-28 solute carrier family 6 member 3 Homo sapiens 123-126 11215759-2 2000 We have recently reported a significant decrease in the density of BZRP labeled by [3H] PK 11195 in the postmortem brain of chronic schizophrenics, suggesting that dysfunctions of the BZRP are involved in the pathophysiology of schizophrenia. Tritium 84-86 translocator protein Homo sapiens 67-71 11215759-2 2000 We have recently reported a significant decrease in the density of BZRP labeled by [3H] PK 11195 in the postmortem brain of chronic schizophrenics, suggesting that dysfunctions of the BZRP are involved in the pathophysiology of schizophrenia. Tritium 84-86 translocator protein Homo sapiens 184-188 10854764-1 2000 We examined the effects of systemic administration of a GABA(A) receptor agonist, muscimol, or antagonist, bicuculline, on the expression of c-Fos protein induced 3h after electrical stimulation of the trigeminal ganglion at low (0.1 mA) or high intensities (1. Tritium 163-165 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 141-146 10660082-4 1999 The SK-N-MC cells were observed to be more susceptible to the supernatants compared to U87-MG with higher inhibition of proliferation as evaluated by [3H]thymidine incorporation (P < 0.05 for 24 and 72 h and P < 0.0005 for 48 and 96 h). Tritium 151-153 small nucleolar RNA, C/D box 87 Homo sapiens 87-90 10582617-4 1999 We measured the oxidation of the catecholamine by following the binding of [3H]DA to the sulfhydryl-rich protein alcohol dehydrogenase. Tritium 76-78 aldo-keto reductase family 1 member A1 Homo sapiens 113-134 10564740-5 1999 5-HT(1B) receptor binding sites ([125I]cyanopindolol) were significantly increased in the hypothalamic arcuate nucleus (ARC) of DiO rats (58%; P<0.05), as were 5-HT(2A) receptor binding sites ([3H]ketanserin) in both the ARC (44%; P<0.05) and lateral hypothalamic area (LHA) (121%; P<0.05). Tritium 197-199 5-hydroxytryptamine receptor 1B Rattus norvegicus 0-7 10556940-5 1999 Using the scintillation proximity assay, [3H]-L-AP4 saturation analysis gave binding parameters K(D) and Bmax values of 150 nM and 9.3 pmoles mg-1 protein, respectively. Tritium 42-44 replication initiator 1 Rattus norvegicus 48-51 10556940-8 1999 Several peptides inhibited the [3H]-L-AP4 binding with the following rank order of potency: glutamate-glutamate>glutamate-glutamate-leucine=aspartate - glutamate>>glutamate - glutamate-aspartate>lactoyl-glutamate>>aspartate-aspartate. Tritium 32-34 replication initiator 1 Rattus norvegicus 38-41 10583213-3 1999 The occurrence of [3H]sphingosine 1-phosphate (Sph-1-P) from [3H]Sph (actively incorporated from the outside) in the blood cells was strong, long-lasting, and independent of cell activation. Tritium 19-21 ankyrin 1 Homo sapiens 47-52 10583213-3 1999 The occurrence of [3H]sphingosine 1-phosphate (Sph-1-P) from [3H]Sph (actively incorporated from the outside) in the blood cells was strong, long-lasting, and independent of cell activation. Tritium 62-64 ankyrin 1 Homo sapiens 47-52 10520140-4 1999 The time course of the effects of the tricyclic antidepressants desipramine and amitriptyline on GR binding, as assessed by [3H]dexamethasone binding using RU 28362, a specific agonist for GR, showed a biphasic mode of stimulation: desipramine significantly increased the GR binding with 2-day exposure by 36% over that in controls and by 99% and 60% with 10- and 14-day exposures, respectively. Tritium 125-127 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 97-99 10598791-3 1999 Total [3H]phosphoinositide (IPx) accumulation, a measure of phospholipase C (PLC) activity, induced by somatostatin (somatotropin release-inhibiting factor, SRIF) and cortistatin (CST) analogues was studied at human somatostatin receptor subtypes 1-5 (hsst1-5) recombinantly expressed in CCL39 (Chinese hamster lung fibroblast) cells. Tritium 7-9 somatostatin Homo sapiens 103-115 10670694-3 1999 Transforming growth factor alpha stimulated [3H]thymidine incorporation and tubulogenesis of RGM1 cells in collagen matrix, both of which were significantly suppressed by treatment with a cyclooxygenase-2 specific inhibitor, NS-398 or cyclooxygenase-2 antisense oligonucleotide. Tritium 45-47 transforming growth factor alpha Rattus norvegicus 0-32 10555184-6 1999 (3H)PAF binding to platelets of stroke patients was lower than in controls (149.58 +/- 46.11 and 212.1 +/- 10.3 receptors cell-1, respectively, p < 0.001) and was significantly correlated with infarct volume (r = -0.606, p = 0.014) and with neurological score (r = 0.527, p = 0.032). Tritium 1-3 carboxyl ester lipase pseudogene Homo sapiens 122-128 10629852-6 1999 Endothelin-1 facilitated the [3H]-L-NMMA uptake by endothelial cell and brought about the potentiation of L-NMMA-mediated inhibition of NO generation. Tritium 30-32 endothelin-1 Oryctolagus cuniculus 0-12 10465291-4 1999 Although beta1-receptors remained detectable by [3H]CGP-12177 binding in the mature brown adipocytes, the cAMP response to NE (based on propranolol inhibitory potency) switched from beta1 to beta3. Tritium 49-51 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 Homo sapiens 9-14 11245086-6 1999 When the concentration of TP40 was 10 or 100 micrograms.L-1, inhibitory effects of TP40 on rapidly proliferating SMC proliferation and protein synthesis were much higher than that on quiescent SMC (P < 0.01), and the IC50 of [3H]leucine incorporation against rapidly proliferating and quiescent SMC were 8.01 (5.05-12.69) and 121.95 (90.98-163.47) micrograms.L-1. Tritium 229-231 CD7 molecule Homo sapiens 26-30 11245086-6 1999 When the concentration of TP40 was 10 or 100 micrograms.L-1, inhibitory effects of TP40 on rapidly proliferating SMC proliferation and protein synthesis were much higher than that on quiescent SMC (P < 0.01), and the IC50 of [3H]leucine incorporation against rapidly proliferating and quiescent SMC were 8.01 (5.05-12.69) and 121.95 (90.98-163.47) micrograms.L-1. Tritium 229-231 CD7 molecule Homo sapiens 83-87 10507691-3 1999 The BK (10 microM) stimulation of cells brought about an increase in conjugated dienes and LDH release only after 4 h. Following 24 h treatment with 50 microM A beta peptide, the [Me-3H]choline incorporation into PtdCho strongly decreased while the [3H]choline release increased, indicating PtdCho hydrolysis. Tritium 183-185 amyloid beta precursor protein Rattus norvegicus 159-165 10507691-3 1999 The BK (10 microM) stimulation of cells brought about an increase in conjugated dienes and LDH release only after 4 h. Following 24 h treatment with 50 microM A beta peptide, the [Me-3H]choline incorporation into PtdCho strongly decreased while the [3H]choline release increased, indicating PtdCho hydrolysis. Tritium 250-252 amyloid beta precursor protein Rattus norvegicus 159-165 10413299-8 1999 Cd2+ pretreatment reduced the [3H]CCPA affinity, but did not modify the affinity of the antagonist [3H]DPCPX, the Bmax remaining unaffected. Tritium 31-33 Cd2 molecule Rattus norvegicus 0-3 10560510-2 1999 STUDY DESIGN: By the combined method of autoradiography with [3H]thymidine and immunohistochemistry using a monoclonal antibody against mouse macrophages (F4/80 or BM8), the labeling rate of [3H]thymidine in macrophages within the liver sinusoids was examined at various intervals after single flash labeling with [3H]thymidine in adult mice with or without daily administration of recombinant human M-CSF. Tritium 192-194 adhesion G protein-coupled receptor E1 Mus musculus 155-160 10560510-2 1999 STUDY DESIGN: By the combined method of autoradiography with [3H]thymidine and immunohistochemistry using a monoclonal antibody against mouse macrophages (F4/80 or BM8), the labeling rate of [3H]thymidine in macrophages within the liver sinusoids was examined at various intervals after single flash labeling with [3H]thymidine in adult mice with or without daily administration of recombinant human M-CSF. Tritium 192-194 adhesion G protein-coupled receptor E1 Mus musculus 155-160 10457264-4 1999 Thrombin or the rat PAR-1-activating peptide SFFLRNPSENTFELVPL (SFFL) stimulated cell proliferation (as assessed by 3H- thymidine incorporation) of primary osteoblast-like cells derived from long bone or calvaria, and treatment with antibodies to PAR-1 abolished the proliferative response to thrombin. Tritium 116-118 coagulation factor II (thrombin) receptor Rattus norvegicus 20-25 10383382-2 1999 When assaying the human recombinant cPLA2 using membranes isolated from [3H]arachidonate-labeled U937 cells as substrate, 2-(2"-benzyl-4-chlorophenoxy)ethyl-dimethyl-n-octadecyl-ammonium chloride (compound 1) was found to inhibit the enzyme in a dose-dependent manner (IC50 = 5 microM). Tritium 73-75 phospholipase A2 group IVA Homo sapiens 36-41 10362643-7 1999 Similar results were observed in binding studies with the CCK-A receptor antagonist [3H]L-364,718. Tritium 85-87 cholecystokinin A receptor Homo sapiens 58-72 10363967-2 1999 Evidence for MRP-mediated antifolate efflux relies upon the following findings: (a) a 2-3.3-fold lower accumulation of [3H]MTX and subsequent reduced formation of long-chain polyglutamate forms of MTX; (b) reversal of MTX resistance by probenecid in both transfectants, and (c) ATP-dependent uptake of [3H]MTX in inside-out vesicles of MRP1 and MRP2 transfectants. Tritium 120-122 ATP binding cassette subfamily C member 2 Homo sapiens 345-349 10425225-4 1999 The peak [3H]thymidine uptake induced by 20 ng/ml of either HGF or EGF was seen after 72 h; however, DNA binding of C/EBP and HNF1 decreased already after 6 h (electrophoretic mobility shift assay). Tritium 10-12 hepatocyte growth factor Rattus norvegicus 60-70 11715432-4 1999 Adm, CNP, CGRP, SST, and PTHrP had no effect on basal 3H-TdR incorporation, but they could inhibit Hcy-induced 3H-TdR incorporation in dose-dependent manner. Tritium 111-113 ADM Oryctolagus cuniculus 0-3 10371159-2 1999 In kidney-derived COS-7, COS-1 and HEK-293 but not in other mammalian cell lines (CHO, Y1, Ltk-), we have characterized a putative functional dopamine transporter displaying a high affinity (Km approximately 250 nM) and a low capacity (approximately 0.1 pmol/10(5) cells/min) for [3H]dopamine uptake. Tritium 281-283 solute carrier family 6 member 3 Homo sapiens 142-162 10220486-4 1999 Intestinal excretion of [3H]IVM and [3H]CSA was enhanced in PGP (+/+) animals. Tritium 25-27 phosphoglycolate phosphatase Mus musculus 60-63 10220486-4 1999 Intestinal excretion of [3H]IVM and [3H]CSA was enhanced in PGP (+/+) animals. Tritium 37-39 phosphoglycolate phosphatase Mus musculus 60-63 10208280-4 1999 Moreover, 100-200 microM suramin stimulates [3H]-thymidine incorporation by those tropoelastin-producing glioma cell lines, but not by A 2058 melanoma cells, which do not produce elastin. Tritium 45-47 elastin Homo sapiens 82-94 10452123-4 1999 RESULTS: Pretreatment of pulmonary VSMC with Cap 1 mumol.L-1 blocked hypoxia-induced increase in cell number and incorporation of [3H]proline and [3H]thymidine, which were decreased 25%, 21%, and 36%, respectively, as compared with hypoxic control. Tritium 131-133 adenylyl cyclase-associated protein 1 Oryctolagus cuniculus 45-50 10452123-4 1999 RESULTS: Pretreatment of pulmonary VSMC with Cap 1 mumol.L-1 blocked hypoxia-induced increase in cell number and incorporation of [3H]proline and [3H]thymidine, which were decreased 25%, 21%, and 36%, respectively, as compared with hypoxic control. Tritium 147-149 adenylyl cyclase-associated protein 1 Oryctolagus cuniculus 45-50 10330690-2 1999 Receptor autoradiography of [3H]-PK11195, a PBR selective ligand, indicated dose-dependent increases throughout the nigrostriatal dopaminergic system as early as 24 h after MPTP administration (10-80 mg/kg), which persisted for at least 21 days. Tritium 29-31 translocator protein Homo sapiens 44-47 10330690-5 1999 DAT levels were measured by [3H]-WIN 35,428 autoradiography, and dopamine content decreased with increasing MPTP dose. Tritium 29-31 solute carrier family 6 member 3 Homo sapiens 0-3 10037192-11 1999 The following LD50s were obtained: [table: see text] The results showed that for both the [3H]thymidine incorporation assay and the clonogenic assay, the combination of IFN/CRA rendered U373 cells more susceptible to ionizing radiation than the untreated control or either single agent alone. Tritium 91-93 myotubularin related protein 11 Homo sapiens 173-176 9950616-5 1999 When apo IRBP was present only in the basal medium, or was absent from the incubation entirely, low levels of [3H]11-cis RAL were released apically and basally. Tritium 111-113 retinol binding protein 3 Bos taurus 9-13 9950616-7 1999 Instead, the enhancement of [3H]11-cis RAL release into the apical, but not the basal, medium in the presence of apo IRBP suggests that [3H]11-cis RAL release is polarized and dependent on the presence of apo IRBP. Tritium 29-31 retinol binding protein 3 Bos taurus 117-121 9950616-7 1999 Instead, the enhancement of [3H]11-cis RAL release into the apical, but not the basal, medium in the presence of apo IRBP suggests that [3H]11-cis RAL release is polarized and dependent on the presence of apo IRBP. Tritium 137-139 retinol binding protein 3 Bos taurus 117-121 9950616-7 1999 Instead, the enhancement of [3H]11-cis RAL release into the apical, but not the basal, medium in the presence of apo IRBP suggests that [3H]11-cis RAL release is polarized and dependent on the presence of apo IRBP. Tritium 137-139 retinol binding protein 3 Bos taurus 209-213 10216761-1 1999 Pretreatment of chicken bone marrow macrophages and embryo fibroblasts with supernatants containing chicken interferon gamma (IFN-gamma) for 24 hr prior to inoculation inhibited intracellular Eimeria tenella replication, measured by [3H] uracil incorporation. Tritium 234-236 interferon gamma Gallus gallus 108-135 9987015-3 1999 PKC activation by phorbol esters resulted in a large facilitation of field-evoked Ca(2+)-dependent [3H]-D-aspartate release and a lesser enhancement of KCl-stimulated release. Tritium 100-102 protein kinase C, gamma Rattus norvegicus 0-3 10188142-3 1999 Competition studies employing some selective agonists for NK1, NK2 and NK3 receptors have demonstrated that only the NK1 selective agonist, [Sar9, Met(O2)11]-SP, was a competitor for [3H]SP binding. Tritium 184-186 NK3 homeobox 1 Homo sapiens 71-74 10193897-6 1999 At the concentrations employed, CRH and VIP both substantially increased intracellular [3H]-cyclic AMP accumulation. Tritium 88-90 corticotropin releasing hormone Mus musculus 32-35 9837923-5 1998 ATP-dependent [3H]GSH transport was cis-inhibited by substrates of the yeast Ycf1p transporter and inhibited by 4,4"-diisothiocyanatostilbene-2,2"-disulfonic acid, probenecid, and sulfinpyrazone, inhibitors of MRP1 and MRP2, but was minimally affected by membrane potential or pH gradient uncouplers. Tritium 15-17 mitochondrial 37S ribosomal protein MRP2 Saccharomyces cerevisiae S288C 219-223 9843686-4 1998 In this study, we show that mammalian p10/NTF2 dramatically inhibits the dissociation of [3H]GDP from Ran and the binding of [35S]GTPgammaS to Ran following the dissociation of non-radioactive GDP by RCC1, the only known mammalian guanine nucleotide exchange factor for Ran (Ran-GEF) [7]. Tritium 90-92 nuclear transport factor 2 Homo sapiens 42-46 9843686-4 1998 In this study, we show that mammalian p10/NTF2 dramatically inhibits the dissociation of [3H]GDP from Ran and the binding of [35S]GTPgammaS to Ran following the dissociation of non-radioactive GDP by RCC1, the only known mammalian guanine nucleotide exchange factor for Ran (Ran-GEF) [7]. Tritium 90-92 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 279-282 9808692-12 1998 Unequivocal evidence that the receptor that modulates nicotine-stimulated [3H]-GABA release contains a beta2 subunit was obtained in a study using wild-type, heterozygous and homozygous beta2 null mutant mice. Tritium 75-77 hemoglobin, beta adult minor chain Mus musculus 103-108 9831908-5 1998 5-HT stimulated [3H]-inositol phosphate ([3H]-IPx) production at both the 5-HT2C (pEC50=8.03+/-0.15) and 5-HT2A receptors (pEC50=7.15+/-0.08), with maximal responses occurring after exposure to 1 microM and 10 microM 5-HT, respectively. Tritium 17-19 5-hydroxytryptamine receptor 2C Homo sapiens 74-80 9831908-5 1998 5-HT stimulated [3H]-inositol phosphate ([3H]-IPx) production at both the 5-HT2C (pEC50=8.03+/-0.15) and 5-HT2A receptors (pEC50=7.15+/-0.08), with maximal responses occurring after exposure to 1 microM and 10 microM 5-HT, respectively. Tritium 42-44 5-hydroxytryptamine receptor 2C Homo sapiens 74-80 9766637-1 1998 Exposure of guinea-pig eosinophils to leukotriene B4 (LTB4; 1 microM) resulted in a rapid generation of H2O2 (index of NADPH oxidase activation), stimulated [3H]arachidonic acid (AA) release (index of phospholipase A2 activity), and promoted CD18-dependent homotypic aggregation. Tritium 158-160 prostaglandin reductase 1 Cavia porcellus 54-58 9765360-6 1998 Oxytocin inhibited [3H]5HT uptake into uterine mast cells during estrus, but not in ovarectomized mice treated with progesterone. Tritium 20-22 oxytocin Mus musculus 0-8 9922982-3 1998 Effect of MX1 (10(-6) M) on mucin biosynthesis measured by [3H] glucosamine incorporation in rat gastric corpus has been determined. Tritium 60-62 solute carrier family 13 member 2 Rattus norvegicus 28-33 9853009-10 1998 The identification of carbaryl as an AhR ligand was demonstrated by its ability to competitively inhibit [3H]-TCDD to the guinea pig hepatic cytosolic AhR. Tritium 106-108 aryl hydrocarbon receptor Cavia porcellus 37-40 9853009-10 1998 The identification of carbaryl as an AhR ligand was demonstrated by its ability to competitively inhibit [3H]-TCDD to the guinea pig hepatic cytosolic AhR. Tritium 106-108 aryl hydrocarbon receptor Cavia porcellus 151-154 9753624-6 1998 Also, UCN (10(-7)M) itself stimulated [3H]leucine uptake into neonatal rat cardiomyocytes and potentiated endothelin-1-induced increase of [3H]leucine uptake. Tritium 39-41 urocortin Rattus norvegicus 6-9 9753624-6 1998 Also, UCN (10(-7)M) itself stimulated [3H]leucine uptake into neonatal rat cardiomyocytes and potentiated endothelin-1-induced increase of [3H]leucine uptake. Tritium 140-142 urocortin Rattus norvegicus 6-9 9764974-9 1998 For [3H]AMPA, significant septotemporal gradients of binding were restricted to layers of CA3, CA4 and the dentate gyrus, with values generally increasing from septal to temporal levels. Tritium 5-7 carbonic anhydrase 3 Rattus norvegicus 90-93 9721195-6 1998 BR and BV not only stimulated AhR transformation and DRE binding in vitro andin cells in culture, but competitive inhibition of [3H]TCDD-specific binding to the cytosolic AhR revealed that these chemicals are AhR ligands. Tritium 129-131 aryl hydrocarbon receptor Rattus norvegicus 171-174 9721195-6 1998 BR and BV not only stimulated AhR transformation and DRE binding in vitro andin cells in culture, but competitive inhibition of [3H]TCDD-specific binding to the cytosolic AhR revealed that these chemicals are AhR ligands. Tritium 129-131 aryl hydrocarbon receptor Rattus norvegicus 171-174 9730917-2 1998 The present study describes the properties of [3H]Ro 63-0563, the first selective 5-HT6 receptor radioligand. Tritium 47-49 5-hydroxytryptamine receptor 6 Rattus norvegicus 82-87 9729282-1 1998 Human cocaine users exhibit increased striatal [3H]WIN35428 binding to the dopamine transporter (DAT). Tritium 48-50 solute carrier family 6 member 3 Homo sapiens 75-95 9729282-1 1998 Human cocaine users exhibit increased striatal [3H]WIN35428 binding to the dopamine transporter (DAT). Tritium 48-50 solute carrier family 6 member 3 Homo sapiens 97-100 9681461-8 1998 A low Mrp activity could be demonstrated in the endothelial cell primary monocultures, because the intracellular [3H]vincristine accumulation was increased by several MRP modulators. Tritium 114-116 ATP binding cassette subfamily C member 2 Rattus norvegicus 6-9 9681461-8 1998 A low Mrp activity could be demonstrated in the endothelial cell primary monocultures, because the intracellular [3H]vincristine accumulation was increased by several MRP modulators. Tritium 114-116 ATP binding cassette subfamily C member 2 Rattus norvegicus 167-170 9660760-5 1998 Experiments on the cultured cells transfected with the nociceptin receptor cDNA showed that NalBzoH competed with [3H]nociceptin binding and attenuated the nociceptin-induced inhibition of cAMP accumulation. Tritium 115-117 opioid receptor-like 1 Mus musculus 55-74 9675089-0 1998 Characterization of [3H]MEN 11420, a novel glycosylated peptide antagonist radioligand of the tachykinin NK2 receptor. Tritium 21-23 tachykinin receptor 2 Homo sapiens 105-117 9675089-2 1998 [3H]MEN 11420 bound to a single class of high affinity binding sites: its binding was inhibited by natural tachykinins (potency ranking: NKA >> SP > or = NKB), as well as by peptide (MEN 11420 > MEN 10376 >> R 396) and nonpeptide (SR 48968 > GR 159897) selective NK2 receptor antagonists. Tritium 1-3 tachykinin receptor 2 Homo sapiens 284-296 9675089-3 1998 These data indicate that [3H]MEN 11420 is a potent radioligand for the human tachykinin NK2 receptor that may represent a useful tool for studying ligand-receptor interactions at the molecular level. Tritium 26-28 tachykinin receptor 2 Homo sapiens 88-100 9655736-6 1998 An antibody to ICAM-1 inhibited HRV-14 infection of epithelial cells and abolished H2O2-induced increases in G and [3H]mannitol flux in IL-1beta-pretreated epithelial cells with HRV-14 infection. Tritium 116-118 intercellular adhesion molecule 1 Homo sapiens 15-21 9692783-4 1998 Specific [3H]-L-AP4 binding to rat brain sections was observed at high densities in the molecular layer of the cerebellar cortex and the outer layer of the superior colliculus. Tritium 10-12 replication initiator 1 Rattus norvegicus 16-19 9692783-6 1998 Low levels of [3H]-L-AP4 binding were found in other regions of the hippocampal formation, in the entorhinal cortex and the granule cell layer of cerebellum. Tritium 15-17 replication initiator 1 Rattus norvegicus 21-24 9662487-5 1998 Four-week male Sprague-Dawley rats with streptozotocin-induced diabetes and age-matched control rats were intravenously injected with [3H]albumin. Tritium 135-137 albumin Rattus norvegicus 138-145 9714507-2 1998 At concentrations of > or = 100 microM valproate decreased binding of 40 nM [3H]AMPA to 45+/-56% of control level (CA 1). Tritium 80-82 carbonic anhydrase 1 Homo sapiens 118-122 9684745-15 1998 Moreover, newly synthesized [3H]retinoic acids were retained within cells bound to CRABP-II. Tritium 29-31 cellular retinoic acid binding protein 2 Homo sapiens 83-91 9636062-0 1998 Secondary tritium and solvent deuterium isotope effects as a probe of the reaction catalyzed by porcine recombinant dihydropyrimidine dehydrogenase. Tritium 10-17 dihydropyrimidine dehydrogenase Homo sapiens 116-147 9633917-16 1998 Treatment of neonatal myocytes with the PKCalpha and PKCepsilon antisense oligodeoxynucleotides for 5 days significantly reduced Ca2+-dependent and Ca2+-independent PKC activity, respectively, as well as the [3H]leucine incorporation induced by myotrophin. Tritium 209-211 protein kinase C, alpha Rattus norvegicus 40-48 9633917-16 1998 Treatment of neonatal myocytes with the PKCalpha and PKCepsilon antisense oligodeoxynucleotides for 5 days significantly reduced Ca2+-dependent and Ca2+-independent PKC activity, respectively, as well as the [3H]leucine incorporation induced by myotrophin. Tritium 209-211 protein kinase C, alpha Rattus norvegicus 40-43 9693780-5 1998 In hippocampal slices preincubated with [3H]choline, the electrically evoked overflow of 3H at S1 increased from 0.11 (P3) to 0.81% of tissue 3H (P16), the latter value being still much lower than that of hippocampal slices from adult rats (2.89% of tissue 3H). Tritium 41-43 cyclin-dependent kinase inhibitor 2A Rattus norvegicus 146-149 9693780-5 1998 In hippocampal slices preincubated with [3H]choline, the electrically evoked overflow of 3H at S1 increased from 0.11 (P3) to 0.81% of tissue 3H (P16), the latter value being still much lower than that of hippocampal slices from adult rats (2.89% of tissue 3H). Tritium 89-91 cyclin-dependent kinase inhibitor 2A Rattus norvegicus 146-149 9693780-5 1998 In hippocampal slices preincubated with [3H]choline, the electrically evoked overflow of 3H at S1 increased from 0.11 (P3) to 0.81% of tissue 3H (P16), the latter value being still much lower than that of hippocampal slices from adult rats (2.89% of tissue 3H). Tritium 89-91 cyclin-dependent kinase inhibitor 2A Rattus norvegicus 146-149 9693780-5 1998 In hippocampal slices preincubated with [3H]choline, the electrically evoked overflow of 3H at S1 increased from 0.11 (P3) to 0.81% of tissue 3H (P16), the latter value being still much lower than that of hippocampal slices from adult rats (2.89% of tissue 3H). Tritium 89-91 cyclin-dependent kinase inhibitor 2A Rattus norvegicus 146-149 9693781-6 1998 In septal slices preincubated with [3H]choline, the electrically evoked overflow of 3H at S1 increased from 0.31% (P3) to 2.10% of tissue 3H (P16), the latter value being still lower than that of septal slices from adult rats (3.46% of tissue 3H). Tritium 36-38 cyclin-dependent kinase inhibitor 2A Rattus norvegicus 142-145 9693781-6 1998 In septal slices preincubated with [3H]choline, the electrically evoked overflow of 3H at S1 increased from 0.31% (P3) to 2.10% of tissue 3H (P16), the latter value being still lower than that of septal slices from adult rats (3.46% of tissue 3H). Tritium 84-86 cyclin-dependent kinase inhibitor 2A Rattus norvegicus 142-145 9693781-6 1998 In septal slices preincubated with [3H]choline, the electrically evoked overflow of 3H at S1 increased from 0.31% (P3) to 2.10% of tissue 3H (P16), the latter value being still lower than that of septal slices from adult rats (3.46% of tissue 3H). Tritium 84-86 cyclin-dependent kinase inhibitor 2A Rattus norvegicus 142-145 9693781-6 1998 In septal slices preincubated with [3H]choline, the electrically evoked overflow of 3H at S1 increased from 0.31% (P3) to 2.10% of tissue 3H (P16), the latter value being still lower than that of septal slices from adult rats (3.46% of tissue 3H). Tritium 84-86 cyclin-dependent kinase inhibitor 2A Rattus norvegicus 142-145 9637489-1 1998 Our previous study showed that CD9 costimulation of TCR-triggered naive T cells elicits activation ([3H]TdR incorporation) that is similar to CD28 costimulation; however, unlike CD28 costimulation, CD9 costimulation results in apoptosis of these previously activated T cells. Tritium 101-103 CD9 molecule Homo sapiens 31-34 9714293-6 1998 Thrombin, thrombin receptor-activating peptide, U46619, collagen, and arachidonic acid each caused the release of [3H]serotonin from dense granules, but only that elicited by low-dose collagen and arachidonic acid was inhibited by PD98059. Tritium 115-117 TRAP Homo sapiens 10-46 9617548-2 1998 METHODS: Proliferation of hRPE cells in the presence of increasing concentrations of bFGF and bFGF + RA was measured by 3H-thymidine incorporation into hRPE cells. Tritium 120-122 ribulose-5-phosphate-3-epimerase Homo sapiens 26-30 9617548-2 1998 METHODS: Proliferation of hRPE cells in the presence of increasing concentrations of bFGF and bFGF + RA was measured by 3H-thymidine incorporation into hRPE cells. Tritium 120-122 ribulose-5-phosphate-3-epimerase Homo sapiens 152-156 9540054-2 1998 In vitro binding of [3H]PK-11195[1-(2-chlorophenyl)-N-methyl-(1-methylpropyl)-3-isoguinol ine carboxamide] to human gliomas in radioligand binding studies revealed a significantly higher level (about 3 fold) of PBR binding sites in both low grade and high grade gliomas as compared to normal cortex. Tritium 21-23 translocator protein Homo sapiens 211-214 9596414-4 1998 Astrocytoma cells also express the cell surface 67-kDa elastin binding protein (EBP), which binds elastin degradation products, leading to the upregulation of cyclin A and cdk2 and increased incorporation of [3H]-thymidine. Tritium 209-211 elastin Homo sapiens 55-62 9596414-4 1998 Astrocytoma cells also express the cell surface 67-kDa elastin binding protein (EBP), which binds elastin degradation products, leading to the upregulation of cyclin A and cdk2 and increased incorporation of [3H]-thymidine. Tritium 209-211 EBP cholestenol delta-isomerase Homo sapiens 80-83 9696277-3 1998 Among the intra-adrenal peptides, vasoactive intestinal peptide (VIP, 100 pmol/l) and neurotensin inhibited [3H]dopamine uptake by approximately 25%. Tritium 109-111 neurotensin Mus musculus 86-97 9622271-13 1998 In co-cultured SMC, FGF-2-SAP significantly decreased both [3H]TdR uptake and total DNA content at 0.5, 5, 50, and 500 ng/ml (0.01-10 nM) compared to positive controls. Tritium 60-62 fibroblast growth factor 2 Canis lupus familiaris 20-25 9622271-14 1998 In co-cultured EC, FGF-2-SAP decreased [3H]TdR uptake at 50 and 500 ng/ml and total DNA content at 500 ng/ml compared to positive controls. Tritium 40-42 fibroblast growth factor 2 Canis lupus familiaris 19-24 9523560-7 1998 The effect of IFN-alpha on [3H]noradrenaline uptake was diminished in protein kinase C-down-regulated cells. Tritium 28-30 interferon alpha-A Bos taurus 14-23 9523560-9 1998 Scatchard analysis of [3H]desipramine binding revealed that IFN-alpha decreased the maximal binding (Bmax) values without any change in the dissociation constant (K(D)) values. Tritium 23-25 interferon alpha-A Bos taurus 60-69 9566579-5 1998 The levels of [3H]forskolin binding were elevated (28-67%) in cortex, thalamus, dentate gyrus, hippocampal CA3 and cerebellum of the pentobarbital withdrawal animals, while these changes were not observed in tolerant rats. Tritium 15-17 carbonic anhydrase 3 Rattus norvegicus 107-110 9566587-6 1998 Furthermore, in primary embryonic mesencephalic cultures, antisense BDNF reduced the number of tyrosine hydroxylase positive neurons and inhibited [3H]DA uptake in a time- and dose-dependent manner. Tritium 148-150 brain-derived neurotrophic factor Rattus norvegicus 68-72 9546654-6 1998 Pharmacological characterization of crude membrane preparations of the recombinant yeast cells showed saturable binding of the glycine antagonist [3H]MDL105,519 with Kd values of 56.9 +/- 6.19 nM (NR1a/NR2A), 26.72 +/- 2.13 nM (NR1a/NR2B), and 21.22 +/- 1.64 nM (NR1a/NR2C), and bound capacities of 17.94 +/- 1.24 pmol/mg membrane protein (NR1a/NR2A), 11.45 +/- 0.67 pmol/mg (NR1a/NR2B), and 16.15 +/- 0.86 (NR1a/NR2C) pmol/mg. Tritium 147-149 glutamate ionotropic receptor NMDA type subunit 2C Rattus norvegicus 268-272 9546654-6 1998 Pharmacological characterization of crude membrane preparations of the recombinant yeast cells showed saturable binding of the glycine antagonist [3H]MDL105,519 with Kd values of 56.9 +/- 6.19 nM (NR1a/NR2A), 26.72 +/- 2.13 nM (NR1a/NR2B), and 21.22 +/- 1.64 nM (NR1a/NR2C), and bound capacities of 17.94 +/- 1.24 pmol/mg membrane protein (NR1a/NR2A), 11.45 +/- 0.67 pmol/mg (NR1a/NR2B), and 16.15 +/- 0.86 (NR1a/NR2C) pmol/mg. Tritium 147-149 glutamate ionotropic receptor NMDA type subunit 2C Rattus norvegicus 413-417 9535013-14 1998 Gamma-mangostin caused a concentration-dependent inhibition of the binding of [3H]-spiperone, a specific 5-HT2A receptor antagonist, to mouse brain membranes. Tritium 78-83 5-hydroxytryptamine (serotonin) receptor 2A Mus musculus 105-120 9593222-6 1998 Moreover, ET-1, ET-3 and SRTX-b completely displaced [3H]BQ-123 with IC50 values of 0.8, 81 and 4.4 nM, respectively, but with slopes of ET-3 and SRTX-b being again shallow. Tritium 54-56 endothelin-1 Oryctolagus cuniculus 10-20 9500202-8 1998 In contrast to downregulation of RFC, the much greater uptake of [3H]MTX was observed in the MOLT-3/trimetrexate (TMQ)800-MTX10000 in parallel with increased RFC1 expression. Tritium 66-68 replication factor C subunit 1 Homo sapiens 158-162 9494030-4 1998 METHODS AND RESULTS: Thrombin (1 U/mL) caused a threefold to fourfold increase of DNA synthesis in cultured bovine coronary artery SMC as assessed from [3H]thymidine incorporation. Tritium 153-155 coagulation factor II, thrombin Bos taurus 21-29 9457843-8 1998 iPD1 competitively inhibited [3H]cPD1 binding to TraA, whereas pheromones and inhibitors relating to other plasmid systems did not. Tritium 30-32 interleukin 1 receptor associated kinase 4 Homo sapiens 0-4 9453571-2 1998 The mRNA levels for nAChR subunits were measured by ribonuclease protection assay, and the number of nAChR isoforms was measured with (-)-[3H]nicotine, [3H]epibatidine, and alpha-[3H]bungarotoxin ([3H]alpha-Bgt). Tritium 139-141 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 101-106 9519253-4 1998 In contrast, in the presence of nifedipine, L-AP4 inhibited the remaining [3H]GABA release, but LY354740 was no longer active. Tritium 75-77 replication initiator 1 Rattus norvegicus 46-49 9417122-2 1998 sPLA2 induced a release of [3H]arachidonic acid ([3H]AA) similar to that elicited by lysophosphatidic acid (LPA), a messenger acting via a G-protein-coupled receptor and a product of sPLA2 on lipid microvesicles. Tritium 28-30 phospholipase A2 group IIA Homo sapiens 0-5 9417122-4 1998 As these findings pointed to a selective mechanism of [3H]AA release, it was hypothesized that sPLA2 could act by a signaling mechanism involving the activation of cytosolic PLA2 (cPLA2), i.e. the type of PLA2 involved in the release of [3H]AA elicited by agonists. Tritium 55-57 phospholipase A2 group IIA Homo sapiens 95-100 9417122-4 1998 As these findings pointed to a selective mechanism of [3H]AA release, it was hypothesized that sPLA2 could act by a signaling mechanism involving the activation of cytosolic PLA2 (cPLA2), i.e. the type of PLA2 involved in the release of [3H]AA elicited by agonists. Tritium 55-57 phospholipase A2 group IVA Homo sapiens 180-185 9417122-4 1998 As these findings pointed to a selective mechanism of [3H]AA release, it was hypothesized that sPLA2 could act by a signaling mechanism involving the activation of cytosolic PLA2 (cPLA2), i.e. the type of PLA2 involved in the release of [3H]AA elicited by agonists. Tritium 55-57 phospholipase A2 group IIA Homo sapiens 96-100 9417122-4 1998 As these findings pointed to a selective mechanism of [3H]AA release, it was hypothesized that sPLA2 could act by a signaling mechanism involving the activation of cytosolic PLA2 (cPLA2), i.e. the type of PLA2 involved in the release of [3H]AA elicited by agonists. Tritium 238-240 phospholipase A2 group IIA Homo sapiens 95-100 9417122-4 1998 As these findings pointed to a selective mechanism of [3H]AA release, it was hypothesized that sPLA2 could act by a signaling mechanism involving the activation of cytosolic PLA2 (cPLA2), i.e. the type of PLA2 involved in the release of [3H]AA elicited by agonists. Tritium 238-240 phospholipase A2 group IVA Homo sapiens 164-178 9417122-4 1998 As these findings pointed to a selective mechanism of [3H]AA release, it was hypothesized that sPLA2 could act by a signaling mechanism involving the activation of cytosolic PLA2 (cPLA2), i.e. the type of PLA2 involved in the release of [3H]AA elicited by agonists. Tritium 238-240 phospholipase A2 group IVA Homo sapiens 180-185 9417122-4 1998 As these findings pointed to a selective mechanism of [3H]AA release, it was hypothesized that sPLA2 could act by a signaling mechanism involving the activation of cytosolic PLA2 (cPLA2), i.e. the type of PLA2 involved in the release of [3H]AA elicited by agonists. Tritium 238-240 phospholipase A2 group IIA Homo sapiens 96-100 9417122-12 1998 In summary, these data indicate the existence in astrocytoma cells of a signaling pathway triggered by engagement of a sPLA2-binding structure, that produces the release of [3H]AA by activating the MAP kinase cascade and cPLA2, and leads to a mitogenic response after longer periods of incubation. Tritium 174-176 phospholipase A2 group IIA Homo sapiens 119-124 9417122-12 1998 In summary, these data indicate the existence in astrocytoma cells of a signaling pathway triggered by engagement of a sPLA2-binding structure, that produces the release of [3H]AA by activating the MAP kinase cascade and cPLA2, and leads to a mitogenic response after longer periods of incubation. Tritium 174-176 phospholipase A2 group IVA Homo sapiens 221-226 10353719-16 1998 L1210/DU5 was used to examine the permeant selectivity of recombinant rat CNT1 by comparing a series of nucleoside analogs with respect to (i) inhibition of inward fluxes of [3H]thymidine, (ii) initial rates of transport of 3H-analog, and (iii) cytotoxicity to L1210/DU5 versus the parental transport-deficient cell line. Tritium 175-177 solute carrier family 28 member 1 Rattus norvegicus 74-78 9496228-2 1998 After 18 weeks of age, decreases of 45% and 40% respectively in insulin- and phorbol ester-stimulated [3H]2-deoxyglucose (DOG) uptake were observed, compared with those in Long-Evans Tokushima (LETO) rats (control). Tritium 103-105 insulin Canis lupus familiaris 64-71 9826113-3 1998 We hypothesized that in AD, the loss of [3H]-ligand binding to nAChRs, which are largely presynaptic, would correlate with changes in two other presynaptic markers: synaptophysin (Syn), a measure of synaptic density, and ChAT activity. Tritium 41-43 synaptophysin Homo sapiens 165-178 9826113-3 1998 We hypothesized that in AD, the loss of [3H]-ligand binding to nAChRs, which are largely presynaptic, would correlate with changes in two other presynaptic markers: synaptophysin (Syn), a measure of synaptic density, and ChAT activity. Tritium 41-43 synaptophysin Homo sapiens 180-183 9826113-3 1998 We hypothesized that in AD, the loss of [3H]-ligand binding to nAChRs, which are largely presynaptic, would correlate with changes in two other presynaptic markers: synaptophysin (Syn), a measure of synaptic density, and ChAT activity. Tritium 41-43 choline O-acetyltransferase Homo sapiens 221-225 9826113-11 1998 Syn correlated with [3H]-EPI binding in AD (r = 0.48, p = 0.006; Pearson) but ChAT did not (r = -0.20, p = 0.34). Tritium 21-23 synaptophysin Homo sapiens 0-3 9435903-5 1997 Importantly, the analogue 16, with the highest affinity for the dopamine transporter (DAT), binds in a monophasic and irreversible manner, as evidenced by the greatly diminished binding of [3H]WIN 35,428 in tissue that had been preincubated with the ligand and then thoroughly washed using centrifugation. Tritium 190-192 solute carrier family 6 member 3 Homo sapiens 64-84 9435903-5 1997 Importantly, the analogue 16, with the highest affinity for the dopamine transporter (DAT), binds in a monophasic and irreversible manner, as evidenced by the greatly diminished binding of [3H]WIN 35,428 in tissue that had been preincubated with the ligand and then thoroughly washed using centrifugation. Tritium 190-192 solute carrier family 6 member 3 Homo sapiens 86-89 9435903-7 1997 This shift in KD to a lower value suggests that the preincubation with 16 produced a preferential irreversible binding to the low-affinity [3H]WIN 35,428 site on the dopamine transporter. Tritium 140-142 solute carrier family 6 member 3 Homo sapiens 166-186 9527511-7 1997 The novel peripheral-type benzodiazepine receptor ligand 2-(4-fluorophenyl)-N,N-di-n-hexyl-1H-indole-3-acetamide (FGIN1-27) displaced only [3H]PK11195 binding with high potency. Tritium 140-142 translocator protein Homo sapiens 10-49 9444399-4 1997 The presence of functional ORL1 in mouse embryonic brain was also confirmed by specific binding of [3H] N/OFQ (kd = 1.3 +/- 0.5 nM and Bmax = 72 +/- 9 fmol/mg protein) as well as by N/OFQ-stimulated G protein activation. Tritium 100-102 opioid receptor-like 1 Mus musculus 27-31 9444399-4 1997 The presence of functional ORL1 in mouse embryonic brain was also confirmed by specific binding of [3H] N/OFQ (kd = 1.3 +/- 0.5 nM and Bmax = 72 +/- 9 fmol/mg protein) as well as by N/OFQ-stimulated G protein activation. Tritium 100-102 prepronociceptin Mus musculus 104-109 9444399-4 1997 The presence of functional ORL1 in mouse embryonic brain was also confirmed by specific binding of [3H] N/OFQ (kd = 1.3 +/- 0.5 nM and Bmax = 72 +/- 9 fmol/mg protein) as well as by N/OFQ-stimulated G protein activation. Tritium 100-102 prepronociceptin Mus musculus 182-187 9372555-6 1997 The Bmax values and KD values for [3H]DPCPX-binding in stratum radiatum/pyramidale of CA1 and the superficial layer of insular cortex were 598 and 430 fmol/mg gray matter and 9.9 and 14.2 nM, respectively. Tritium 35-37 carbonic anhydrase 1 Homo sapiens 86-89 9570379-2 1997 This method measures directly the binding of a 3H-labeled phosphopeptide derived from the sequence around tyrosine317 in the human Shc (SpYVNVK) to the SH2 domain of Grb2, which is precoated as glutathione S-transferase fusion proteins on solid phase. Tritium 47-49 SHC adaptor protein 1 Homo sapiens 131-134 9371525-5 1997 Reduction of HSP-70 was not associated with decreased protein synthesis, as demonstrated by [3H] leucine incorporation. Tritium 93-95 heat shock protein family A (Hsp70) member 4 Homo sapiens 13-19 9351366-5 1997 When apoA-I- and apoE-deficient mice received both chow- and fat-rich diets, their plasmas released significantly less 3H-cholesterol from radiolabeled fibroblasts than did plasma of normal mice. Tritium 119-121 apolipoprotein A-I Mus musculus 5-11 9332884-9 1997 The density of [3H]U69,593 binding sites in MaTLE and PTLE patients was highest in areas CA1 and the subiculum-regions having little or no DYN-staining. Tritium 16-18 carbonic anhydrase 1 Homo sapiens 89-92 9299527-1 1997 Synthetic melittin inhibited the enzymatic activity of secretory phospholipase A2 (PLA2) from various sources, including bee and snake venoms, bovine pancreas, and synovial fluid from rheumatoid arthritis patients, irrespective of substrate (e.g., [14C]-phosphatidylcholine or phosphatidylethanolamine vesicles and [3H]-oleic acid-labeled E.coli). Tritium 316-318 phospholipase A2 group IIA Homo sapiens 83-87 9316847-2 1997 When Xenopus oocytes expressing the cloned human dopamine transporter (hDAT) were pretreated with bath-applied phorbol 12-myristate 13-acetate (PMA), a PKC activator, [3H]DA uptake decreased irreversibly in a time- and dose-dependent manner (IC50 = 22 nM; maximal inhibition = 63-85%). Tritium 168-170 solute carrier family 6 member 3 Homo sapiens 49-69 9284886-2 1997 Pretreatment with dexamethasone (DEX) and prednisolone for 60 minutes resulted in 50% inhibition of insulin-induced [3H]2-deoxyglucose (DOG) uptake at 10(-8) and 10(-7) mol/L, respectively, in rat adipocytes and 20% and 25% inhibition of insulin-induced [3H]2-DOG uptake, respectively, in soleus muscles. Tritium 117-119 insulin Canis lupus familiaris 100-107 9284886-2 1997 Pretreatment with dexamethasone (DEX) and prednisolone for 60 minutes resulted in 50% inhibition of insulin-induced [3H]2-deoxyglucose (DOG) uptake at 10(-8) and 10(-7) mol/L, respectively, in rat adipocytes and 20% and 25% inhibition of insulin-induced [3H]2-DOG uptake, respectively, in soleus muscles. Tritium 255-257 insulin Canis lupus familiaris 100-107 9277376-2 1997 In rats treated with T3, the beta 3-AR density (assessed by the binding of [3H]CGP-12177) showed a decrease of 50%, as did their mRNA, as analyzed by reverse transcriptase-polymerase chain reaction. Tritium 76-78 adrenoceptor beta 3 Rattus norvegicus 29-38 9260907-2 1997 Here we report on retinol uptake through the RBP-TTR complex by primary cultured rat hepatocytes (parenchymal cells, PC) and nonparenchymal cells (NPC) following incubation with [3H]retinol-RBP or the [3H]retinol-RBP-TTR complex under several conditions. Tritium 179-181 transthyretin Rattus norvegicus 49-52 9260907-2 1997 Here we report on retinol uptake through the RBP-TTR complex by primary cultured rat hepatocytes (parenchymal cells, PC) and nonparenchymal cells (NPC) following incubation with [3H]retinol-RBP or the [3H]retinol-RBP-TTR complex under several conditions. Tritium 202-204 transthyretin Rattus norvegicus 49-52 9260907-6 1997 The uptake of [3H]retinol from protein-bound retinol was inhibited by an excess of either retinol-RBP or retinol-RBP-TTR complex. Tritium 15-17 transthyretin Rattus norvegicus 117-120 9258354-5 1997 The binding affinities of these compounds were determined at the dopamine transporter for the displacement of [3H]WIN-35428. Tritium 111-113 solute carrier family 6 member 3 Homo sapiens 65-85 9218468-7 1997 Although CEA could not mediate bile acid efflux in COS cells cotransfected with IBAT and CEA, efflux of [3H]taurocholate was detected in COS cells cotransfected with IBAT and a chimeric molecule having the carboxyl-terminal tail and membrane spanning domain of CBATP and the amino-terminal extracellular tail of CEA. Tritium 105-108 solute carrier family 10 member 2 Rattus norvegicus 166-170 9224735-2 1997 In the presence of [3H]RA both RAR alpha and RXR alpha existed primarily as monomers of 50 kDa in solutions containing 80 mM KCl. Tritium 20-22 retinoid X receptor alpha Homo sapiens 45-54 9279477-3 1997 Pretreatment with 1 microM TPA and 10 nM insulin for 60 min resulted in the marked decreases of insulin-induced [3H]2-DOG uptake. Tritium 113-115 insulin Canis lupus familiaris 41-48 9279477-3 1997 Pretreatment with 1 microM TPA and 10 nM insulin for 60 min resulted in the marked decreases of insulin-induced [3H]2-DOG uptake. Tritium 113-115 insulin Canis lupus familiaris 96-103 9228186-5 1997 Chronic treatments had no effect on presynaptic 5-HT1B autoreceptors, functionally evaluated by measuring 5-HT1B-mediated inhibition of depolarization-induced [3H]5-HT release from cortical and hippocampal synaptosomes. Tritium 160-162 5-hydroxytryptamine receptor 1B Rattus norvegicus 106-112 9182536-5 1997 The mDP receptor bound only [3H]PGD2 with a Kd value of 43 nM. Tritium 29-31 prostaglandin D2 synthase (brain) Mus musculus 32-36 9182536-8 1997 This chimeric IPN-V/DPVI-C receptor acquired the ability to bind [3H]PGD2 and [3H]PGE2 without decreasing the affinities of the mIP receptor to [3H]iloprost and [3H]PGE1. Tritium 66-68 prostaglandin D2 synthase (brain) Mus musculus 69-73 9286814-4 1997 In the in vitro incubation system of rat gastric mucosa, histamine and dibutyryl cyclic AMP significantly increased [3H]-labeled mucin in the corpus. Tritium 117-119 solute carrier family 13 member 2 Rattus norvegicus 129-134 9185839-0 1997 [3H]R-terazosin binds selectively to alpha1-adrenoceptors over alpha2-adrenoceptors - comparison with racemic [3H]terazosin and [3H]prazosin. Tritium 1-3 adrenoceptor alpha 2B Rattus norvegicus 63-83 9194577-6 1997 In mouse, rat and guinea pig pancreatic acini, murine Swiss 3T3 cells and human SCLC cells, GRP-related peptides caused a 600%, 500%, 250%, 300% and 60% increase, respectively, in [3H]IP with 1-3 nM causing a half-maximal effect. Tritium 181-183 gastrin releasing peptide Homo sapiens 92-95 9185331-3 1997 [3H]IP3 bound to a single high-affinity site, IP3-R. Tritium 1-3 inositol 1,4,5-trisphosphate receptor, type 1 Rattus norvegicus 46-51 9221731-2 1997 This was proved by ultracentrifugation of the protein fraction from rabbit liver in the presence of the 3H-labeled inhibitor, 3 beta-(2-hydroxy-2-[3H]ethoxy)-5 alpha-cholest-8(14)-en-15-one, or by the substitution of the [3H]-25-hydroxycholesterol in its complex with the oxysterol binding protein. Tritium 104-106 oxysterol-binding protein 1 Oryctolagus cuniculus 272-297 9134502-5 1997 In addition, physiological concentrations of vasopressin cause a synergistic stimulation of [3H]thymidine uptake in rat liver cells in the presence of HGF. Tritium 93-95 hepatocyte growth factor Rattus norvegicus 151-154 9113087-4 1997 The reduction in dopamine transporter activity by PMA was caused by a decrease in the Vmax value of [3H]dopamine uptake, opposed by a smaller reduction in the Km value, whereas the effect of PMA on [3H]WIN 35,428 binding was caused by a reduction in the Bmax value without a change in the Kd value. Tritium 101-103 solute carrier family 6 member 3 Homo sapiens 17-37 9113087-4 1997 The reduction in dopamine transporter activity by PMA was caused by a decrease in the Vmax value of [3H]dopamine uptake, opposed by a smaller reduction in the Km value, whereas the effect of PMA on [3H]WIN 35,428 binding was caused by a reduction in the Bmax value without a change in the Kd value. Tritium 199-201 solute carrier family 6 member 3 Homo sapiens 17-37 9030597-6 1997 Using high affinity [3H]ryanodine binding as the experimental approach, we show that this mutation enhances the sensitivity of RYR1 to activating concentrations of Ca2+ and to the exogenous and diagnostically used ligands caffeine and 4-chloro-m-cresol. Tritium 21-23 carbonic anhydrase 2 Homo sapiens 164-167 8994059-4 1997 A beta impaired 3H-deoxy-glucose transport in a concentration-dependent manner and with a time course preceding neurodegeneration. Tritium 16-18 amyloid beta precursor protein Rattus norvegicus 0-6 9023275-11 1997 A similar interaction was found between A1 receptor-mediated reduction and nAChR-mediated positive feedback modulation of [3H]ACh release. Tritium 123-125 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 75-80 9017664-0 1997 Cholecystokinin-GABA interactions in rodent cortex: analyses of cholecystokinin effects on K(+)- and L-glutamate-induced release of [3H]GABA from rat cortical slices and cultured mouse cortical neurones. Tritium 133-135 cholecystokinin Rattus norvegicus 64-79 9203642-1 1997 A thrombin receptor-radioligand binding assay was developed using [3H]A(pF-F)R(ChA)(hR)Y-NH2 ([3H]haTRAP), a high affinity thrombin receptor-activating peptide (TRAP), and human platelet membranes. Tritium 67-69 TRAP Homo sapiens 123-159 9203642-1 1997 A thrombin receptor-radioligand binding assay was developed using [3H]A(pF-F)R(ChA)(hR)Y-NH2 ([3H]haTRAP), a high affinity thrombin receptor-activating peptide (TRAP), and human platelet membranes. Tritium 67-69 TRAP Homo sapiens 100-104 9203642-4 1997 Various TRAPs and a TRAP antagonist, but not other receptor agonists, displaced [3H]haTRAP from the binding sites. Tritium 81-83 TRAP Homo sapiens 8-12 9005998-7 1997 The mutant VDR had an eightfold lower affinity for [3H]1,25(OH)2D3 than the normal VDR when measured at 24 degrees C. RFLP demonstrated that the patient was homozygous for the mutation while the parents were heterozygous. Tritium 52-54 vitamin D receptor Homo sapiens 11-14 9067639-6 1997 Thrombin also strongly stimulated (over 100 fold) the incorporation of [3H]thymidine into growth arrested PA fibroblasts which was inhibited by rapamycin (by 33.6 +/- 2.0%). Tritium 72-74 coagulation factor II, thrombin Bos taurus 0-8 9479632-4 1997 Addition of histamine (10(-7)-10(-5) M) to the culture medium increased [3H]GlcN-labeled mucin in the corpus tissue in a concentration-dependent manner. Tritium 73-75 solute carrier family 13 member 2 Rattus norvegicus 89-94 8978713-5 1997 However, coaddition of K-252b (EC50 of approximately 2 nM) with 10-100 ng/ml NT-3 led to incorporation of [3H]thymidine in trkA expressing cells to a level induced by optimal concentrations of nerve growth factor (NGF). Tritium 107-109 neurotrophic receptor tyrosine kinase 1 Rattus norvegicus 123-127 9016355-3 1997 Chemical modifications of either the base or the pentose ring strongly decrease the inhibitory potency of L-dCyd, L-dCyd is resistant to cytidine deaminase and competes in cell cultures with the natural D-dCyd as substrate for dCK, thus reducing the incorporation of exogenous [3H]dCyd into DNA. Tritium 278-280 Calcium/calmodulin-dependent protein kinase II Drosophila melanogaster 227-230 9015331-0 1997 [3H]1,3-di(2-tolyl)guanidine and [3H](+)pentazocine binding sites in the rat brain: autoradiographic visualization of the putative sigma1 and sigma2 receptor subtypes. Tritium 1-4 sigma non-opioid intracellular receptor 1 Rattus norvegicus 131-157 9015331-0 1997 [3H]1,3-di(2-tolyl)guanidine and [3H](+)pentazocine binding sites in the rat brain: autoradiographic visualization of the putative sigma1 and sigma2 receptor subtypes. Tritium 1-3 sigma non-opioid intracellular receptor 1 Rattus norvegicus 131-157 8982496-18 1996 It was concluded that, in addition to the type I organic cation hepatic transporter, [3H]-MPP+ is taken up by rat hepatocytes through P-glycoprotein, a canalicular transport system that usually excretes endobiotics and xenobiotics. Tritium 86-88 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 134-148 8996809-0 1996 Phenylglycines can evoke quisqualate-primed depolarizations in rat cingulate cortex: an effect associated with [3H]DL-AP4 uptake. Tritium 112-114 replication initiator 1 Rattus norvegicus 118-121 8937455-0 1996 Binding domains for blockers and substrates on the cloned human dopamine transporter studied by protection against N-ethylmaleimide-induced reduction of 2 beta-carbomethoxy-3 beta-(4-fluorophenyl)[3H]tropane ([3H]WIN 35,428) binding. Tritium 197-199 solute carrier family 6 member 3 Homo sapiens 64-84 8952052-8 1996 The initial [3H]oleic acid esterification into triacylglycerols and cholesteryl esters was increased 3.9- and 2.5-fold in I-FABP expressing cells. Tritium 13-15 fatty acid binding protein 2, intestinal Mus musculus 122-128 8910293-3 1996 Vesicular [3H]ACh accumulation increased approximately 20 times over levels attained by the endogenous rat VAChT, expressed at low levels in control PC-12 cells. Tritium 11-13 solute carrier family 18 member A3 Rattus norvegicus 107-112 8938656-2 1996 The delta-opioid receptor agonist DPDPE and the kappa-opioid receptor agonist U50488 reduced the evoked [3H]-overflow (acetylcholine release) in a concentration-dependent fashion; the delta-opioid receptor antagonist naltrindole and the kappa-opioid receptor antagonist norbinaltorphimine, respectively, antagonized these effects. Tritium 105-107 opioid receptor kappa 1 Homo sapiens 48-69 8938656-2 1996 The delta-opioid receptor agonist DPDPE and the kappa-opioid receptor agonist U50488 reduced the evoked [3H]-overflow (acetylcholine release) in a concentration-dependent fashion; the delta-opioid receptor antagonist naltrindole and the kappa-opioid receptor antagonist norbinaltorphimine, respectively, antagonized these effects. Tritium 105-107 opioid receptor kappa 1 Homo sapiens 237-258 8957240-6 1996 [3H]Fananserin can be used to label the recombinant dopamine D4 receptor expressed in Chinese hamster ovary cells with a KD of 0.725 nM. Tritium 1-3 dopamine receptor D4 Rattus norvegicus 52-72 8863819-6 1996 [3H]5-HT-moduline binds to a single population of sites in mammalian brain (Kd = 0.4 nM in rat, Kd = 0.8 nM in guinea pig) as well as in transfected cells expressing the 5-HT1B or the 5-HT1D beta receptors (Kd = 0.2 and 0.6 nM, respectively). Tritium 1-3 5-hydroxytryptamine receptor 1B Rattus norvegicus 170-176 8863819-9 1996 The interaction of 5-HT-moduline with the 5-HT1B/1D receptor corresponds to a decrease in the functional activity of the receptor (i.e., a decrease in the inhibitory effect of a 5-HT1B agonist on the evoked release of [3H]5-HT from synaptosomal preparation). Tritium 219-221 5-hydroxytryptamine receptor 1B Rattus norvegicus 42-48 8863819-9 1996 The interaction of 5-HT-moduline with the 5-HT1B/1D receptor corresponds to a decrease in the functional activity of the receptor (i.e., a decrease in the inhibitory effect of a 5-HT1B agonist on the evoked release of [3H]5-HT from synaptosomal preparation). Tritium 219-221 5-hydroxytryptamine receptor 1B Rattus norvegicus 178-184 9387786-4 1996 IGF-II could enhance 3H-leu incorporation into protein and 3H-TdR incorporation into DNA of the luteal cells, but these effects could be inhibited by CYX and Act D. The above results suggest that stimulating effect of IGF-II on progesterone production by rat luteal cells may be related to enhanced protein and DNA synthesis. Tritium 21-23 insulin-like growth factor 2 Rattus norvegicus 0-6 8997162-3 1996 The exposure to a high glucose concentration for 7 days significantly inhibited Ang II(10(-6) M)-induced [3H]thymidine uptake, compared to normal glucose concentration (5 mM)(M +/- SD., 1050 +/- 100 cpm/well vs 550 +/- 97, p < 0.05), and markedly prevented the inhibition of [3H]thymidine uptake by TGF-beta(1 ng/ml)(132 +/- 10 vs 340 +/- 67, p < 0.05). Tritium 106-108 angiogenin Rattus norvegicus 80-83 8997162-3 1996 The exposure to a high glucose concentration for 7 days significantly inhibited Ang II(10(-6) M)-induced [3H]thymidine uptake, compared to normal glucose concentration (5 mM)(M +/- SD., 1050 +/- 100 cpm/well vs 550 +/- 97, p < 0.05), and markedly prevented the inhibition of [3H]thymidine uptake by TGF-beta(1 ng/ml)(132 +/- 10 vs 340 +/- 67, p < 0.05). Tritium 279-281 angiogenin Rattus norvegicus 80-83 8798532-6 1996 Here we report that the complete inhibition of p22(phox) mRNA expression by stable transfection of antisense p22(phox) cDNA into VSMCs results in a decrease in cytochrome b content, which is accompanied by a significant inhibition of angiotensin II-stimulated NADH/NADPH-dependent superoxide production, subsequent hydrogen peroxide production, and [3H]leucine incorporation. Tritium 350-352 cytochrome b, mitochondrial Rattus norvegicus 160-172 8797588-6 1996 LY335979 blocked [3H]azidopine photoaffinity labeling of the M(r) approximately 170,000 Pgp in CEM/VLB100 plasma membranes and competitively inhibited equilibrium binding of [3H]vinblastine to Pgp (Ki of approximately 0.06 microM). Tritium 18-20 phosphoglycolate phosphatase Mus musculus 88-91 8872305-9 1996 Together, these data provide a molecular correlate for chronic postnatal PCP-induced down-regulation of [3H]MK-801 binding in rat cerebral cortex and suggest that the NR2B subunit plays an important role in developmental plasticity. Tritium 105-107 glutamate ionotropic receptor NMDA type subunit 2B Rattus norvegicus 167-171 8772729-9 1996 Glycerol phosphate shuttle flux, as estimated by [2-3H]glycerol conversion to [3H]H2O, was increased to 120-130% by infection with Adex1CAmGPDH, but not with Adex1CAcGPDH infection, in both MIN6 and HIT cells. Tritium 78-82 glycerol phosphate dehydrogenase 2, mitochondrial Mus musculus 131-143 8886616-7 1996 After a low intravenous dose to CD4+ mice, rapid loss of [3H]CE9.1 from plasma (mean residence time < 1 hr) was accompanied by accumulation of radioactivity in the spleen (a maximum of 18% of the administered dose at 2 hr). Tritium 58-60 CD4 antigen Mus musculus 32-35 8830781-6 1996 Transformants overexpressing the cAMP receptor gene cAR1 show a 10-fold increase of [3H]cAMP binding and a similar increase of [125I]CMF binding; disruption of the cAR1 gene abolishes both cAMP and CMF binding. Tritium 85-87 nuclear receptor subfamily 1 group I member 3 Homo sapiens 52-56 8830781-6 1996 Transformants overexpressing the cAMP receptor gene cAR1 show a 10-fold increase of [3H]cAMP binding and a similar increase of [125I]CMF binding; disruption of the cAR1 gene abolishes both cAMP and CMF binding. Tritium 85-87 nuclear receptor subfamily 1 group I member 3 Homo sapiens 164-168 8843076-8 1996 Likewise, transient transfection of NMB cells with the plasmid expressing dopamine transporter complementary DNA in the antisense orientation partially blocked [3H]dopamine uptake. Tritium 161-163 solute carrier family 6 member 3 Homo sapiens 74-94 8904956-2 1996 Dosing with RA (100 mg/kg body weight) for 96 hours and exposure to [3H]RA enhanced the levels of radioactive incorporation of several nuclear matrix proteins, including p51, and p55, similarly in Y/AL and O/DR rats. Tritium 69-71 MAGUK p55 scaffold protein 1 Rattus norvegicus 179-182 8871096-1 1996 The distribution of the [3H]alpha-amino-3-hydroxy-5-methylisoxzalepropionic acid (AMPA) receptor subunit GluR4 was studied in frontal, parietal and temporal cerebral cortex, subcortical white matter and corpus callosum of neonatal, immature and mature rats. Tritium 25-27 glutamate ionotropic receptor AMPA type subunit 4 Rattus norvegicus 105-110 8840278-0 1996 Autoradiographic localization of vasopressin V1a receptors in the rat kidney using [3H]-SR 49059. Tritium 84-86 arginine vasopressin receptor 1A Rattus norvegicus 45-48 8840278-1 1996 Localization and characterization of binding sites of the selective non-peptide vasopressin receptor V1a ligand, [3H]-SR 49059, were investigated in the adult rat kidney by quantitative autoradiography using a fast-detecting radioluminographic phosphor-imaging plate system. Tritium 114-116 arginine vasopressin receptor 1A Rattus norvegicus 101-104 8840278-2 1996 [3H]-SR 49059, like the other V1a ligands used, showed a total absence of binding in the papilla, discrete and sparse labeling in the cortex and maximal binding in the outer part of the inner medulla. Tritium 1-3 arginine vasopressin receptor 1A Rattus norvegicus 30-33 8840278-6 1996 The relative potencies of the reference peptide and non-peptide compounds to inhibit [3H]-SR 49059 binding confirm the V1a nature of the site and the stereospecificity of this binding. Tritium 86-88 arginine vasopressin receptor 1A Rattus norvegicus 119-122 8840278-7 1996 Thus, [3H]-SR 49059 allows the mapping and characterization of the V1a receptor population present in the rat kidney. Tritium 7-9 arginine vasopressin receptor 1A Rattus norvegicus 67-70 8840290-1 1996 We studied the specific binding of radiolabeled bradykinin ([3H]BK) and vasopressin ([3H]AVP) to membrane preparations of bovine and porcine kidney medulla. Tritium 61-63 kininogen 1 Bos taurus 48-58 8840290-5 1996 In contrast, the specific binding of [3H]BK, but not [3H]AVP, to porcine kidney medulla (Kd = 0.32 nM, Bmax = 45 fmol/mg) was dependent upon the presence of enzyme inhibitors to prevent the rapid and selective degradation of bradykinin. Tritium 38-40 kininogen 1 Bos taurus 225-235 9389195-4 1996 It was found that ET-1 produced concentration- and time-dependent increases in 3H-TdR incorporation and in MAPK activity of these cells. Tritium 79-81 endothelin-1 Oryctolagus cuniculus 18-22 8755535-5 1996 By preloading recombinant Ran/TC4 with [gamma-32P]GTP or [3H]GDP, we show that the interactions with p97 and NTF2 are specific for the GTP- and GDP-bound forms, respectively. Tritium 58-60 nuclear transport factor 2 Homo sapiens 109-113 8755601-4 1996 The expression of KOR in microglial cells was confirmed by membrane binding of [3H]U69,593, a kappa-selective ligand, and by indirect immunofluorescence. Tritium 80-82 opioid receptor kappa 1 Homo sapiens 18-21 8660698-2 1996 ATP also causes a threefold increase of arachidonic acid (AA) metabolite release from [3H]AA-preloaded EATC, indicating that, under these experimental conditions, it induces phospholipase A2 (PLA2) activation. Tritium 87-89 phospholipase A2, group IB, pancreas Mus musculus 192-196 8713072-3 1996 The production of recombinant cNT1rat was examined by immunoblotting using an epitope-tagged construct and by analysis of inward fluxes of 3H-labelled nucleosides. Tritium 139-141 solute carrier family 28 member 1 Rattus norvegicus 30-34 8836876-6 1996 Following 15 min cell labelling, part of the [3H]alkylacyl-GPC was recovered in plasma membranes indicating a rapid redistribution of the acylated compound. Tritium 46-48 glycophorin C (Gerbich blood group) Homo sapiens 59-62 8836876-9 1996 The [3H]alkylacyl-GP and [3H]alkylacyl-GPethanol, phospholipase D breakdown products from [3H]alkylacyl-GPC, obtained after neutrophil prelabelling and activation by phorbol myristate acetate, were exclusively present in the plasma membranes. Tritium 5-7 glycophorin C (Gerbich blood group) Homo sapiens 104-107 8836876-9 1996 The [3H]alkylacyl-GP and [3H]alkylacyl-GPethanol, phospholipase D breakdown products from [3H]alkylacyl-GPC, obtained after neutrophil prelabelling and activation by phorbol myristate acetate, were exclusively present in the plasma membranes. Tritium 26-28 glycophorin C (Gerbich blood group) Homo sapiens 104-107 8836876-9 1996 The [3H]alkylacyl-GP and [3H]alkylacyl-GPethanol, phospholipase D breakdown products from [3H]alkylacyl-GPC, obtained after neutrophil prelabelling and activation by phorbol myristate acetate, were exclusively present in the plasma membranes. Tritium 26-28 glycophorin C (Gerbich blood group) Homo sapiens 104-107 8690788-4 1996 ANP decreased surface [3H]ALDO binding by approximately 50% after a 2.5-h lag period but had no effect on crypt ALDO binding. Tritium 23-25 natriuretic peptide A Rattus norvegicus 0-3 8670165-6 1996 The effect of activators and inhibitors on mucin secretion was studied by measuring the secretion of [3H]N-acetyl-D-glucosamine-labelled glycoproteins. Tritium 102-104 mucin Canis lupus familiaris 43-48 8780834-2 1996 CHO cells, transfected with a cDNA clone for the 5-HT2C receptor, expressed 287 fmol/mg of the receptor protein as determined by mianserin-sensitive [3H]-mesulergine binding (kd = 0.49 nM). Tritium 150-152 5-hydroxytryptamine receptor 2C Homo sapiens 49-55 8828572-1 1996 The in vivo relationship between the amounts of tryptophan hydroxylase (TPH) protein and its intrinsic synthetic activity, measured by quantifying the amounts of alpha-[3H]methyl-5-hydroxytryptamine (alpha-[3H]M5-HT), is reported in cell body and terminal areas of intact and disturbed serotonergic neurons following a unilateral 5,7-dihydroxytryptamine (5,7-DHT) lesion of the dorsolateral hypothalamus. Tritium 169-171 tryptophan hydroxylase 1 Rattus norvegicus 72-75 8828572-3 1996 On the side contralateral to the lesion, TPH and alpha-[3H]M5-HT levels in the intact hemi-DRN exhibited a caudo-rostral distribution and were positively and significantly correlated (p < or = 0.001); the calculated TPH-specific activity was 0.76 nCi of alpha-[3H]M5-HT formed per U TPH. Tritium 264-266 tryptophan hydroxylase 1 Rattus norvegicus 41-44 8662851-3 1996 Phorbol 12-myristate 13-acetate treatment enhanced high potassium-induced [3H]-norepinephrine release, and a 28-kDa protein recognized by an anti-SNAP-25 antibody was phosphorylated on Ser residues. Tritium 75-77 synaptosome associated protein 25 Rattus norvegicus 146-153 8675188-1 1996 We previously identified that Y" bile acid binders (3alpha-hydroxysteroid dehydrogenases) interact with bile acids in intact rat hepatocytes using [3beta-3H, C24-14C]bile acids and that indomethacin, a competitive inhibitor of 3alpha-hydroxysteroid dehydrogenase, inhibits 3H-loss from the C3-position of bile acids as well as inhibits hepatic bile acid removal and excretion. Tritium 154-156 aldo-keto reductase family 1, member C14 Rattus norvegicus 52-87 8683296-5 1996 Additionally, the biodistribution of two compounds which bind to Glut, 7-[[(2-(3-(125I-p-hydroxyphenyl)propionyl)aminoethyl)amino]carbonyl]-7-+ ++desacetyl-forskolin([125I]HPP forskolin) and [3H]cytochalasin B, were studied in a tumor model which overexpressed Glut. Tritium 192-194 solute carrier family 2 member 1 Homo sapiens 65-69 8723710-4 1996 D-Amphetamine, which releases DA from neurons and blocks the DA transporter directly, inhibited striatal [3H]WIN 35,428 binding in dose-dependent manner. Tritium 106-108 solute carrier family 6 member 3 Homo sapiens 61-75 8621160-6 1996 On the other hand, Ro31-8425 decreased HGF-induced [3H]arachidonic acid (AA) release and [3H]thymidine incorporation. Tritium 52-54 hepatocyte growth factor Rattus norvegicus 39-42 8621160-8 1996 Moreover, a commonly used phospholipase A2 (PLA2) inhibitor, quinacrine, decreased HGF-induced [3H]AA release and [3H]thymidine incorporation. Tritium 96-98 hepatocyte growth factor Rattus norvegicus 83-86 8621160-8 1996 Moreover, a commonly used phospholipase A2 (PLA2) inhibitor, quinacrine, decreased HGF-induced [3H]AA release and [3H]thymidine incorporation. Tritium 115-117 hepatocyte growth factor Rattus norvegicus 83-86 8786427-10 1996 Microglia were also responsive to neurotrophins: brain-derived neurotrophic factor (BDNF) and NT-3 increased [3H]thymidine incorporation in vitro, and NT-3 promoted proliferation. Tritium 110-112 brain derived neurotrophic factor Homo sapiens 49-82 8786427-10 1996 Microglia were also responsive to neurotrophins: brain-derived neurotrophic factor (BDNF) and NT-3 increased [3H]thymidine incorporation in vitro, and NT-3 promoted proliferation. Tritium 110-112 brain derived neurotrophic factor Homo sapiens 84-88 8786427-10 1996 Microglia were also responsive to neurotrophins: brain-derived neurotrophic factor (BDNF) and NT-3 increased [3H]thymidine incorporation in vitro, and NT-3 promoted proliferation. Tritium 110-112 neurotrophin 3 Homo sapiens 94-98 8804051-0 1996 [3H]YM-09151-2 (nemonapride), a potent radioligand for both sigma 1 and sigma 2 receptor subtypes. Tritium 1-3 sigma non-opioid intracellular receptor 1 Rattus norvegicus 60-88 8804051-3 1996 Although Scatchard analysis fitted a one-site model, competition between [3H]YM-09151-2 and (+)-pentazocine revealed two sites, sigma 1 and sigma 2 receptors, at which the Ki values of YM-09151-2 were 8.4 nM and 9.6nM, respectively. Tritium 74-76 sigma non-opioid intracellular receptor 1 Rattus norvegicus 128-157 8804051-5 1996 [3H]YM-09151-2 is, therefore, a potent and useful radioligand for sigma 1/sigma 2 receptor subtypes. Tritium 1-3 sigma non-opioid intracellular receptor 1 Rattus norvegicus 66-90 8626515-3 1996 Results from a nuclear labeling assay using [3H]thymidine suggest that most hepatocytes in primary culture consist of two cell populations that differ in response to FGF-1; one is an FGF-1-responsive cell population, and the other is an EGF-responsive (but not FGF-1-responsive) cell population. Tritium 45-47 fibroblast growth factor 1 Rattus norvegicus 183-188 8626515-3 1996 Results from a nuclear labeling assay using [3H]thymidine suggest that most hepatocytes in primary culture consist of two cell populations that differ in response to FGF-1; one is an FGF-1-responsive cell population, and the other is an EGF-responsive (but not FGF-1-responsive) cell population. Tritium 45-47 fibroblast growth factor 1 Rattus norvegicus 183-188 8800954-4 1996 [3H]Thymidine incorporation of neuritogenic P2- and encephalitogenic myelin basic protein (MBP)-specific T-cell lines declined after 48 h in culture with astrocytes. Tritium 1-3 myelin basic protein Rattus norvegicus 69-89 8800954-4 1996 [3H]Thymidine incorporation of neuritogenic P2- and encephalitogenic myelin basic protein (MBP)-specific T-cell lines declined after 48 h in culture with astrocytes. Tritium 1-3 myelin basic protein Rattus norvegicus 91-94 8613968-8 1996 FR139317 at 10(-5) M decreased the ET-3-induced increase in inositol phosphates by about 60%, whereas it attenuated the increase in [3H]IP1 induced by ET-1 (3 x 10(-8)M) by only 20%. Tritium 133-135 endothelin-1 Oryctolagus cuniculus 151-155 8626384-4 1996 Monocytes cultured in the presence of 10 microM antisense oligonucleotide for 48 h showed a marked decrease (57 +/- 5%; n = 4) of cPLA2 expression, as evaluated by Western blot analysis and a nearly complete inhibition (81.8 +/- 4.2%; n = 3) of [3H]arachidonic acid release in MCP-1-stimulated cells. Tritium 246-248 phospholipase A2 group IVA Homo sapiens 130-135 8626384-9 1996 These data show that cPLA2 plays a major role in [3H]arachidonic acid release by MCP-1 in human monocytes and provide direct evidence for the involvement of cPLA2 in C-C chemokine-induced monocyte chemotaxis. Tritium 50-52 phospholipase A2 group IVA Homo sapiens 21-26 8774152-3 1996 In addition, the constitutive and agonist-stimulated mucin secretion of these cells was studied using a [3H]N-acetyl-D-glucosamine labeling assay, and the stimulation of intracellular cAMP was measured. Tritium 105-107 mucin Canis lupus familiaris 53-58 8617786-8 1996 [3H]Ryanodine binding gave an estimate of Ryr3 which would be only 2% or less of total RyR in rabbit brain. Tritium 1-3 ryanodine receptor 3 Oryctolagus cuniculus 42-46 8643087-3 1996 5-HT2C receptor ligands modulated [3H]thymidine incorporation, DNA amounts, and cell number in serum-starved NIH-3T3 fibroblasts transfected with 5-HT2C receptor cDNA. Tritium 35-37 5-hydroxytryptamine receptor 2C Homo sapiens 0-6 8643087-7 1996 The rank order of potencies of inverse agonists to decrease basal [3H]thymidine incorporation was consistent with their rank order to decrease basal 5-HT2C receptor-mediated phosphoinositide hydrolysis. Tritium 67-70 5-hydroxytryptamine receptor 2C Homo sapiens 149-155 8780000-7 1996 PAF also increased [3H]thymidine incorporation into pericytes by 79%. Tritium 20-22 PCNA clamp associated factor Rattus norvegicus 0-3 8555236-4 1996 On addition of [3H]Sph to intact platelets, the label was rapidly converted to Sph-1-P. Tritium 16-18 ankyrin 1 Homo sapiens 79-84 8555236-5 1996 This conversion into [3H]Sph-1-P was inhibited by N,N-dimethylsphingosine (DMS) in a dose-dependent manner, but not by other structurally related Sph derivatives, including ceramide. Tritium 22-24 ankyrin 1 Homo sapiens 25-30 8772539-7 1996 The antioxidant N-acetyl-L-cysteine (NAC), like surfactant, inhibited [3H]thymidine incorporation. Tritium 71-73 X-linked Kx blood group Homo sapiens 37-40 8550051-6 1996 Infusion of EGF, HGF, uPA, or any combination thereof for up to 7 days resulted in increased numbers of [3H]thymidine-labeled ductal and periductal cells expanding into the liver acinus. Tritium 105-107 hepatocyte growth factor Rattus norvegicus 17-20 8558445-12 1996 These results demonstrate that stable expression of the human alpha 4 beta 2 nAChR subunit combination can give rise to functional ion channels that bind [3H](-)-cytisine with high affinity, exhibit homologous regulation and evoke agonist-induced cation flux with pharmacological properties consistent with native neuronal alpha 4 beta 2 nAChR. Tritium 155-157 immunoglobulin binding protein 1 Homo sapiens 323-330 8614277-0 1996 Characterization and distribution of binding sites for [3H]-SR 141716A, a selective brain (CB1) cannabinoid receptor antagonist, in rodent brain. Tritium 56-58 cannabinoid receptor 1 Rattus norvegicus 91-94 8614277-7 1996 Regional rat brain distribution of CB1 cannabinoid receptors detected by [3H]-SR 141716A saturation binding and autoradiographic studies, showed that this distribution was very similar to that found for [3H]-CP 55,940. Tritium 74-76 cannabinoid receptor 1 Rattus norvegicus 35-38 8614281-4 1996 Positive clones (HN2 kappa 24 and CHO kappa 18) from both lines showed high expression of the kappa opioid receptor, as identified by [3H] U-69,593 binding to membranes prepared from HN2 kappa 24 and CHO kappa 18. Tritium 135-137 opioid receptor kappa 1 Homo sapiens 94-115 8938684-2 1996 Adrenomedullin (10(-10)-10(-7) M inhibited both [3H]thymidine incorporation into cultured rat mesangial cells and cell proliferation in a concentration-dependent manner. Tritium 49-51 adrenomedullin Rattus norvegicus 0-14 8883018-6 1996 Lipocortin-1 inhibited CHMC proliferation in a dose-dependent manner as determined by [3H]thymidine uptake and cell count. Tritium 87-89 annexin A1 Homo sapiens 0-12 8787784-2 1995 BP-42 (0.3-3 micrograms/ml) inhibited the PDGF-BB (30 ng/ml)-stimulated [3H]thymidine incorporation of the SMC in a concentration-dependent manner. Tritium 73-75 Blood pressure QTL 42 Rattus norvegicus 0-5 8787784-3 1995 BP-42 inhibited both the competence and progression phases of [3H]thymidine incorporation induced by PDGF-BB. Tritium 63-65 Blood pressure QTL 42 Rattus norvegicus 0-5 8787784-4 1995 Using the competence assay, BP-42 (0.3-10 micrograms/ml) delayed PDGF-BB (30 ng/ml)-accelerated starting time of [3H]thymidine incorporation in a concentration-dependent manner, confirming that BP-42 inhibited PDGF-BB-induced competence phase of DNA synthesis of SMC. Tritium 114-116 Blood pressure QTL 42 Rattus norvegicus 28-33 8787784-5 1995 BP-42 (1-10 micrograms/ml) also delayed basic fibroblast growth factor (bFGF: 30 ng/ml)-accelerated starting time of [3H]thymidine incorporation. Tritium 118-120 Blood pressure QTL 42 Rattus norvegicus 0-5 8770651-1 1995 Barbiturates are allosteric modulators of the CNS GABAA receptor, increasing [3H]-GABA binding to its receptor sites. Tritium 78-80 gamma-aminobutyric acid type A receptor gamma3 subunit Gallus gallus 50-64 8750916-9 1995 Given alone, CNQX and GAMS, but not AP5 and NBQX, slightly increased the evoked overflow of tritium; the increases were abolished in the presence of adenosine deaminase. Tritium 92-99 adenosine deaminase Oryctolagus cuniculus 149-168 7487907-7 1995 Thapsigargin-induced emptying of intracellular Ca2+ pools elicited the formation of both [3H]PtdBut and [3H]PtdOH and displayed a requirement for extracellular Ca2+. Tritium 90-92 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 47-50 7487907-7 1995 Thapsigargin-induced emptying of intracellular Ca2+ pools elicited the formation of both [3H]PtdBut and [3H]PtdOH and displayed a requirement for extracellular Ca2+. Tritium 105-107 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 47-50 7664666-6 1995 Incubation of [3H]pro-TRH with the intermediate lobe secretory vesicle membrane preparation was followed by immunoprecipitation with antibodies specific for various regions of the pro-TRH sequence, and the immunoprecipitates were analyzed by sodium dodecyl sulfate-gel electrophoresis. Tritium 15-17 thyrotropin releasing hormone Mus musculus 18-25 8538377-3 1995 I-FABP and L-FABP expression preferentially increased [3H]-oleic acid incorporation into triacylglycerols by 5.5-fold and 3.8-fold, respectively. Tritium 55-57 fatty acid binding protein 2, intestinal Mus musculus 0-6 8538377-3 1995 I-FABP and L-FABP expression preferentially increased [3H]-oleic acid incorporation into triacylglycerols by 5.5-fold and 3.8-fold, respectively. Tritium 55-57 fatty acid binding protein 1, liver Mus musculus 11-17 8745475-7 1995 It was demonstrated that the antisense Ki-ras did inhibit the cell growth rate and 3H-TdR incorporation rate of PC-2 cells. Tritium 83-85 KRAS proto-oncogene, GTPase Homo sapiens 39-45 7543820-6 1995 The binding sites were saturated with about 19,700 hyaluronan molecules (Mr 1.4 x 10(6)) bound per cell with a Kd of 0.16 x 10(-9) M. CD44 molecules were responsible for the binding activity since Hermes-1 antibodies that block the binding of hyaluronan to CD44 blocked the binding of [3H]hyaluronan to U-1752 cells. Tritium 286-288 CD44 molecule (Indian blood group) Homo sapiens 134-138 7543820-9 1995 The fact that CD44 molecules are able to bind [3H]hyaluronan only on the SCLC line U-1752 but not on other lung carcinoma cell lines may be of value as a marker for squamous cell carcinoma differentiation. Tritium 47-49 CD44 molecule (Indian blood group) Homo sapiens 14-18 7595638-4 1995 Only with PDGF-AB and -BB a mitogenic effect was observed, while PDGF-neutralizing antibodies produced a reduction of [3H]thymidine incorporation. Tritium 119-121 platelet derived growth factor subunit B Homo sapiens 65-69 7669888-5 1995 SR vesicle-45Ca2+ flux, single-channel, and [3H]ryanodine bind measurements showed that, at < or = 0.2 microM Ca2+, calmodulin activated the Ca2+ release channel severalfold. Tritium 45-47 calmodulin Oryctolagus cuniculus 119-129 7661037-4 1995 Frozen sections of rat cornea were subjected to in situ hybridization using 3H-labeled Mn-SOD cDNA probe. Tritium 76-78 superoxide dismutase 2 Rattus norvegicus 87-93 7494090-4 1995 The FTase is assayed as the transfer of [3H]farnesyl group from [3H]FPP to the ras p21 at pH 7.4 and 37 degrees C in the presence of rat brain cytosol followed by the binding of radioactive farnesylated ras p21 to the phosphocellulose paper. Tritium 41-43 KRAS proto-oncogene, GTPase Rattus norvegicus 83-86 7666953-4 1995 The Michaelis-Menten kinetics assay of CPH in CCV reveals a Km = 28.9 microM and Vmax = 1.88 nmol/min/mg at pH 4.5 using 3H.Bz-FAR as a substrate. Tritium 121-123 carboxypeptidase E Bos taurus 39-42 7479575-4 1995 In the guinea pig kidney [3H]-MK 912 bound to a single saturable site with Kd 8.34 nM and capacity 285 fmol/mg protein, the site showing pharmacological properties like an alpha 2B-adrenoceptor. Tritium 26-28 alpha-2B adrenoceptor Cavia porcellus 172-193 7659284-6 1995 These results raise the possibility that A beta affects calcium homeostasis by a direct action on [3H]Ins(1,4,5)P3 and [3H]Ins(1,3,4,5)P4 receptor sites. Tritium 99-101 amyloid beta precursor protein Rattus norvegicus 41-47 7659284-6 1995 These results raise the possibility that A beta affects calcium homeostasis by a direct action on [3H]Ins(1,4,5)P3 and [3H]Ins(1,3,4,5)P4 receptor sites. Tritium 120-122 amyloid beta precursor protein Rattus norvegicus 41-47 7744827-7 1995 Combination of PE and PYY resulted in increases in [Ca2+]i which were at best additive, whereas they promoted a clearly synergistic rise in Ins(1,4,5)P3 at both 15 and 60 s. Co-stimulation also resulted in a synergistic activation of both protein kinase C (PKC) and [3H]arachidonic acid release. Tritium 267-269 peptide YY Cricetulus griseus 22-25 7772680-2 1995 Dopamine D1-like receptors, probably belonging to the dopamine D1 receptor subtype, were demonstrated in sections of rabbit lung using [3H]-SCH 23390 as a ligand. Tritium 136-138 D(1A) dopamine receptor Oryctolagus cuniculus 54-74 7546018-6 1995 As an effect of calcium entry after PAF application, a translocation of protein kinase C (PKC) toward plasma membranes was demonstrated by 3H-labeled phorbol-binding method. Tritium 139-141 PCNA clamp associated factor Rattus norvegicus 36-39 7596683-5 1995 Bioactive IL-10 was demonstrated by the finding that human milk inhibited [3H]thymidine uptake by human blood lymphocytes and that inhibition was partly overcome by concomitant incubation with antibodies to human IL-10. Tritium 75-77 interleukin 10 Homo sapiens 10-15 7702575-2 1995 YC-1 (1-100 microM) dose-dependently inhibited [3H]thymidine incorporation into DNA in rat A10 VSMCs that were synchronized by serum depletion and then restimulated by addition of 10% foetal calf serum (FCS), whereas FCS-induced [3H]thymidine incorporation into rat synchronized endothelial cells was unaffected by this agent. Tritium 48-50 glutathione S-transferase alpha 1 Rattus norvegicus 0-4 7781713-5 1995 Dopamine D4 receptor density was, because of the absence of a dopamine D4 receptor specific [3H]ligand, determined by the difference between the number of binding sites for [3H]nemonapride and [3H]raclopride. Tritium 174-176 dopamine receptor D4 Rattus norvegicus 0-20 7781713-5 1995 Dopamine D4 receptor density was, because of the absence of a dopamine D4 receptor specific [3H]ligand, determined by the difference between the number of binding sites for [3H]nemonapride and [3H]raclopride. Tritium 174-176 dopamine receptor D4 Rattus norvegicus 0-20 7773187-6 1995 The results strongly suggest that Hb may suppress physiological function(s) of ram p25 in vivo by inhibiting both [32P]GTP-hydrolysis and [3H]GDP-dissociation of ram p25. Tritium 139-141 tubulin polymerization promoting protein Homo sapiens 83-86 7773187-6 1995 The results strongly suggest that Hb may suppress physiological function(s) of ram p25 in vivo by inhibiting both [32P]GTP-hydrolysis and [3H]GDP-dissociation of ram p25. Tritium 139-141 tubulin polymerization promoting protein Homo sapiens 166-169 7775862-4 1995 Pretreatment with 25-OHC (1 microgram/ml) caused a 30% reduction in [3H]cholesterol efflux from L-cells during 8 h of incubation with HDL3. Tritium 69-71 high density lipoprotein (HDL) level 3 Mus musculus 134-138 7539898-0 1995 Prostacyclin receptor in the brain and central terminals of the primary sensory neurons: an autoradiographic study using a stable prostacyclin analogue [3H]iloprost. Tritium 153-155 prostaglandin I2 receptor Rattus norvegicus 0-21 7864797-4 1995 50% by the tyrosine kinase inhibitor genistein, whereas pretreatment with genistein and the PKC inhibitor Ro-31-8220 completely abolished the generation of [3H]PtdBut in response to a maximal concentration of bombesin. Tritium 157-159 gastrin releasing peptide Homo sapiens 209-217 7768260-3 1995 [3H]BQ-123 binding was sensitive to endothelin-1 and endothelin-2 (Ki values = 0.058 and 0.10 nM, respectively) and the endothelin ETA receptor-selective antagonist BQ-123 (Ki = 3.3 nM), while showing low affinity for endothelin-3 (Ki = 50 nM), the endothelin ETB receptor-selective agonist BQ-3020 (Ki = 970 nM) and other bioactive peptides. Tritium 1-3 endothelin 2 Homo sapiens 53-65 7768260-3 1995 [3H]BQ-123 binding was sensitive to endothelin-1 and endothelin-2 (Ki values = 0.058 and 0.10 nM, respectively) and the endothelin ETA receptor-selective antagonist BQ-123 (Ki = 3.3 nM), while showing low affinity for endothelin-3 (Ki = 50 nM), the endothelin ETB receptor-selective agonist BQ-3020 (Ki = 970 nM) and other bioactive peptides. Tritium 1-3 endothelin 3 Homo sapiens 218-230 7532419-6 1995 HGF stimulated [3H]thymidine incorporation into type II cells in primary cultures. Tritium 16-18 hepatocyte growth factor Rattus norvegicus 0-3 7757259-8 1995 In addition, relative to the control, NT-4/5 increased the neuronal differentiation as evidenced by increases in varicosity density and [3H]mazindol binding by 114 and 101% respectively. Tritium 137-139 neurotrophin 4 Rattus norvegicus 38-44 7529746-17 1995 On the other hand, 3H-labeled taxol bound to both J774.1 cells and J7.DEF3 cells in similar time- and dose-dependent manners. Tritium 19-21 RNA binding motif protein 6 Mus musculus 70-74 7860743-7 1995 Hepatic glucose production, measured with [3-3H]glucose, rose from 2.6 +/- 0.2 to 4.3 +/- 0.4 mg/kg per min (P < 0.01) in response to head insulin infusion but remained unchanged (2.6 +/- 0.5 mg/kg per min) during peripheral insulin infusion. Tritium 45-47 insulin Canis lupus familiaris 142-149 7751813-4 1995 Moreover, H2O2-dependent PAF synthesis was measured by high performance liquid chromatography (HPLC) ([3H]acetate incorporation) and radioimmunoassay. Tritium 103-106 PCNA clamp associated factor Rattus norvegicus 25-28 7530955-4 1995 [3H]RX821002 was displaced from HEL membranes by adrenergic drugs with the order of potency being yohimbine approximately oxymetazoline >> prazosin = 2-[2-[4-(o-methoxyphenyl)piperazin-1-yl]ethyl]-4,4-dimethyl- 1,3(2H,4H)-isochinolindione HCl (ARC 239), consistent with this site being an alpha 2A-adrenoceptor. Tritium 1-3 adrenoceptor alpha 2A Homo sapiens 295-316 7713168-2 1995 GR159897 inhibited binding of the NK2 receptor antagonist radioligand [3H]cyclohexylcarbonyl-Gly-Ala-(D)Trp-Phe-NMe2 ([3H]GR100679) to human ileum NK2 receptors transfected into Chinese hamster ovary cells (pKi 9.5) and to rat colon membranes (pKi 10.0). Tritium 71-73 tachykinin receptor 2 Homo sapiens 34-46 7713168-2 1995 GR159897 inhibited binding of the NK2 receptor antagonist radioligand [3H]cyclohexylcarbonyl-Gly-Ala-(D)Trp-Phe-NMe2 ([3H]GR100679) to human ileum NK2 receptors transfected into Chinese hamster ovary cells (pKi 9.5) and to rat colon membranes (pKi 10.0). Tritium 71-73 tachykinin receptor 2 Homo sapiens 34-37 7713168-2 1995 GR159897 inhibited binding of the NK2 receptor antagonist radioligand [3H]cyclohexylcarbonyl-Gly-Ala-(D)Trp-Phe-NMe2 ([3H]GR100679) to human ileum NK2 receptors transfected into Chinese hamster ovary cells (pKi 9.5) and to rat colon membranes (pKi 10.0). Tritium 119-121 tachykinin receptor 2 Homo sapiens 34-46 7713168-2 1995 GR159897 inhibited binding of the NK2 receptor antagonist radioligand [3H]cyclohexylcarbonyl-Gly-Ala-(D)Trp-Phe-NMe2 ([3H]GR100679) to human ileum NK2 receptors transfected into Chinese hamster ovary cells (pKi 9.5) and to rat colon membranes (pKi 10.0). Tritium 119-121 tachykinin receptor 2 Homo sapiens 34-37 8713359-2 1995 In vitro receptor binding autoradiography was used to examine the pattern and intensity of [3H]muscimol binding to GABAA receptors. Tritium 92-94 gamma-aminobutyric acid (GABA) A receptor, subunit gamma 1 Mus musculus 115-120 7811474-8 1995 Both [125I]SP-A and [3H]DPPC were lost exponentially from the total lungs, with half-life values of 6.5 h for SP-A and 12 h for DPPC (P < 0.01). Tritium 21-23 pulmonary surfactant-associated protein A Oryctolagus cuniculus 110-114 7811474-10 1995 In lamellar bodies, 125I and 3H radioactivities per mumol saturated phosphatidylcholine (Sat PC) increased in parallel and were highest at 2 h. Whereas radiolabeled lipids were recovered in lamellar bodies for up to 16 h, iodinated SP-A was lost, indicating less recycling of SP-A than DPPC. Tritium 29-31 pulmonary surfactant-associated protein A Oryctolagus cuniculus 232-236 7811474-10 1995 In lamellar bodies, 125I and 3H radioactivities per mumol saturated phosphatidylcholine (Sat PC) increased in parallel and were highest at 2 h. Whereas radiolabeled lipids were recovered in lamellar bodies for up to 16 h, iodinated SP-A was lost, indicating less recycling of SP-A than DPPC. Tritium 29-31 pulmonary surfactant-associated protein A Oryctolagus cuniculus 276-280 7811475-6 1995 Moreover, treatment either with antisense oligodeoxynucleotides directed against cyclin D2 mRNA or with genistein (a tyrosine kinase inhibitor) caused significant inhibition of [3H]thymidine incorporation into DNA as well as inhibition of cyclin D2 expression in normal 19-day fetal rat AEC. Tritium 178-180 cyclin D2 Rattus norvegicus 81-90 8612042-2 1995 Inactivation of [3H]PLP-reconstituted GABA-AT with 1 followed by denaturation gave no PMP or enamine adduct to the PLP; however, a new unknown metabolite was observed which was identical to the metabolite formed upon inactivation of GABA-AT by L-cycloserine. Tritium 17-19 proteolipid protein 1 Homo sapiens 20-23 8774990-7 1995 However, TGF alpha had already stimulated tritiated thymidine (3H-TdR) incorporation into DNA of gastric epithelial cells at 18 h, suggesting that the promotional effect of TGF alpha on cell migration is a result of stimulated cell proliferation. Tritium 63-65 protransforming growth factor alpha Oryctolagus cuniculus 9-18 8774990-7 1995 However, TGF alpha had already stimulated tritiated thymidine (3H-TdR) incorporation into DNA of gastric epithelial cells at 18 h, suggesting that the promotional effect of TGF alpha on cell migration is a result of stimulated cell proliferation. Tritium 63-65 protransforming growth factor alpha Oryctolagus cuniculus 173-182 7707287-1 1995 The role of oestradiol in the control of uterine responsiveness to oxytocin was investigated by measuring oxytocin-induced phospholipase C activation in [3H]inositol-labelled cultured human myometrial cells. Tritium 154-156 oxytocin/neurophysin I prepropeptide Homo sapiens 67-75 7707287-1 1995 The role of oestradiol in the control of uterine responsiveness to oxytocin was investigated by measuring oxytocin-induced phospholipase C activation in [3H]inositol-labelled cultured human myometrial cells. Tritium 154-156 oxytocin/neurophysin I prepropeptide Homo sapiens 106-114 8688196-2 1995 Potent inhibition of HSF-PLA2 in vitro can be observed with compounds such as scalaradial and ellagic acid, which both have IC50 values of 0.02 microM (using autoclaved [3H]-arachidonic-acid (AA)-labelled Escherichia coli membranes as substrate). Tritium 170-172 phospholipase A2 group IIA Homo sapiens 25-29 7888492-6 1994 OTr assays were accomplished by isolating uterine cell membranes and performing saturation analysis with cold OTAs and tritium-labeled oxytocin. Tritium 119-126 oxytocin/neurophysin I prepropeptide Homo sapiens 135-143 7988425-5 1994 Insulin increased GEF activity in both fractions (48 +/- 12% [3H]GDP released vs. 24 +/- 6% in control plasma membranes, and 65 +/- 13% vs. 13 +/- 4% in control cytosolic fractions), whereas EGF enhanced only the plasma membrane-associated activity (43 +/- 12% of [3H]GDP release in the plasma membrane fraction and 10 +/- 2% in the cytosol). Tritium 62-64 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 18-21 7988425-5 1994 Insulin increased GEF activity in both fractions (48 +/- 12% [3H]GDP released vs. 24 +/- 6% in control plasma membranes, and 65 +/- 13% vs. 13 +/- 4% in control cytosolic fractions), whereas EGF enhanced only the plasma membrane-associated activity (43 +/- 12% of [3H]GDP release in the plasma membrane fraction and 10 +/- 2% in the cytosol). Tritium 265-267 Ras protein specific guanine nucleotide releasing factor 1 Homo sapiens 18-21 7699198-5 1994 Transcription kinetics of the beta-actin and c-myc genes in serum-stimulated fibrosarcoma HT-1080 cells determined by [3H]uridine incorporation were comparable to that determined by the nuclear run-off method. Tritium 119-121 POTE ankyrin domain family member F Homo sapiens 30-40 7632753-1 1994 Oxamniquine (OXY), a tetrahydroquinoline derivative, is used as an antischistosomal drug and generally has been labeled with carbon-14 and tritium. Tritium 139-146 oxytocin Mus musculus 13-16 7964745-13 1994 The restricted localization of high-affinity [3H]ifenprodil binding sites to regions rich in NMDAR2B subunit mRNA may explain the atypical nature of this NMDA antagonist. Tritium 46-48 glutamate ionotropic receptor NMDA type subunit 2B Rattus norvegicus 93-100 7964753-2 1994 Amounts of MARCKS protein, measured by [3H]myristate labeling and western blotting, were severalfold higher in rat C6 glioma and human HTB-11 (SK-N-SH) neuroblastoma cells than in HTB-10 (SK-N-MC) or mouse N1E-115 neuroblastoma cells. Tritium 40-42 myristoylated alanine rich protein kinase C substrate Rattus norvegicus 11-17 7877137-6 1994 [3H]NKA bound to both NK-2Rs with high affinity (KD = 2-7 nM) and saturable (Bmax = 633-9000 fmol/mg protein) manner which was inhibited by GTP analogs. Tritium 1-3 hepatocyte growth factor Mus musculus 22-26 7996850-10 1994 There was significant attenuation of TGF-alpha (50 nM)-stimulated [3H]Thy in the presence of AVP (10 nM). Tritium 67-69 transforming growth factor alpha Rattus norvegicus 37-46 7802697-7 1994 Concentration-dependent curves suggested that the [3H]DFP binding sites differ from the active sites of acetylcholinesterase, butyrylcholinesterase, and neuropathy target esterase, as well as from muscarinic acetylcholine receptors. Tritium 51-53 butyrylcholinesterase Gallus gallus 126-179 7977651-4 1994 With insoluble [3H] elastin as a substrate, elastin-degrading activity could be detected in the culture medium. Tritium 16-18 elastin Homo sapiens 20-27 7977651-4 1994 With insoluble [3H] elastin as a substrate, elastin-degrading activity could be detected in the culture medium. Tritium 16-18 elastin Homo sapiens 44-51 7525818-6 1994 In both case, the binding of [3H]hyaluronan was specifically inhibited by the addition of monoclonal antibody directed against CD44. Tritium 30-32 CD44 molecule (Indian blood group) Homo sapiens 127-131 7526046-15 1994 Cross-sectional autoradiography demonstrated an FGF-1-induced mitotic index increase at 30 days, 9.6 +/- 4.4% compared to 2.5 +/- 1.0 and 0 +/- 0%, respectively, with both myofibroblasts and endothelial cells incorporating the [3H]thymidine label. Tritium 228-230 fibroblast growth factor 1 Canis lupus familiaris 48-53 7933125-11 1994 The methyltransferase activities in the P15 fractions of SFV-infected BHK cells and AcNPV-nsP1-infected Sf9 cells and in E. coli catalyzed linear incorporation of the [3H]methyl group from S-adenosylmethionine to GTP for a 60-min period. Tritium 168-170 SH2 domain containing 3A Homo sapiens 90-94 7948036-3 1994 Interaction of CCK-8 with the very low affinity state of the CCK receptor was measured as CCK-8-inhibitable binding of [3H]L-364,718. Tritium 120-122 cholecystokinin Rattus norvegicus 15-18 7948036-3 1994 Interaction of CCK-8 with the very low affinity state of the CCK receptor was measured as CCK-8-inhibitable binding of [3H]L-364,718. Tritium 120-122 cholecystokinin Rattus norvegicus 61-64 7948036-3 1994 Interaction of CCK-8 with the very low affinity state of the CCK receptor was measured as CCK-8-inhibitable binding of [3H]L-364,718. Tritium 120-122 cholecystokinin Rattus norvegicus 61-64 7842510-3 1994 [3H]CHA binding was greatest in CA1 and CA3 hippocampus in both caffeine-exposed and control rats across all ages. Tritium 1-3 carbonic anhydrase 1 Homo sapiens 32-35 7875557-7 1994 Chronic treatment with the agonists quipazine and trifluoromethylphenylpiperazine (TFMPP) and the antagonists ritanserin and methiothepin significantly down-regulated brainstem 5-HT1C sites, which were 65% of [3H]mesulergine-labeled sites in brainstem. Tritium 210-212 5-hydroxytryptamine receptor 2C Rattus norvegicus 177-183 7845533-4 1994 In normal brain, [3H]-Tf binding was highest in the pyramidal cell layers with CA2 > dentate gyrus granule cell layer > or = CA1 > CA3 > or = CA4 > subiculum > parahippocampal gyrus. Tritium 18-20 carbonic anhydrase 2 Homo sapiens 79-82 7845533-4 1994 In normal brain, [3H]-Tf binding was highest in the pyramidal cell layers with CA2 > dentate gyrus granule cell layer > or = CA1 > CA3 > or = CA4 > subiculum > parahippocampal gyrus. Tritium 18-20 carbonic anhydrase 1 Homo sapiens 125-128 7845533-5 1994 In AD, significant reductions in [3H]-Tf binding were found in CA1, CA2 and CA4 pyramidal cell layers. Tritium 34-36 carbonic anhydrase 1 Homo sapiens 63-66 7845533-5 1994 In AD, significant reductions in [3H]-Tf binding were found in CA1, CA2 and CA4 pyramidal cell layers. Tritium 34-36 carbonic anhydrase 2 Homo sapiens 68-71 8077234-4 1994 TGF-alpha, AR, HB-EGF, and EGF were equipotent in stimulating [3H]thymidine incorporation by RIE-1 cells and bound the EGFr with equivalent affinity. Tritium 63-65 transforming growth factor alpha Rattus norvegicus 0-9 7524992-5 1994 The SNP-stimulated [3H]-cyclic GMP response peaked after 5 min in the presence and absence of the phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine (IBMX). Tritium 20-22 5'-nucleotidase, cytosolic II Homo sapiens 31-34 7524992-7 1994 In the absence of IBMX, forskolin (3 x 10(-5) M) significantly increased [3H]-cyclic GMP levels to 118.5 +/- 8.7% of basal values (P < 0.05, n = 8), and significantly increased both the SNP- and ANP-stimulated [3H]-cyclic GMP accumulation at all concentrations of SNP and ANP used. Tritium 74-76 5'-nucleotidase, cytosolic II Homo sapiens 85-88 7853354-1 1994 Kinetic analysis of the time course of association of [3H]-2,3,7,8-tetrachlorodibenzo-p-dioxin with hepatic cytosol from five rodent species gave additional evidence for differences in the properties of the Ah receptor ligand binding subunit between species. Tritium 54-57 aryl hydrocarbon receptor Rattus norvegicus 207-218 8046464-6 1994 3H-5-HT uptake (0.3 microM 5-HT, 3.4 microCi/ml) in both tissue-printed GFAP(+) astrocytes and NF(+) structures was sensitive to 1 microM fluoxetine and was also Na+ dependent. Tritium 0-2 glial fibrillary acidic protein Rattus norvegicus 72-76 8078497-0 1994 Characterization of a new, highly specific, beta 3-adrenergic receptor radioligand, [3H]SB 206606. Tritium 85-87 adrenoceptor beta 3 Rattus norvegicus 44-70 8078497-1 1994 The RR-enantiomer of the beta 3-adrenergic receptor agonist BRL 37344 was tritiated to yield a high specific activity compound, [3H]SB 206606. Tritium 129-131 adrenoceptor beta 3 Rattus norvegicus 25-51 8078497-9 1994 The similarity of the Kd values and of the Ki values for various beta-adrenergic agonists and antagonists in both systems tested indicates that, in a complex membrane system, [3H]SB 206606 binds selectively to the beta 3-adrenergic receptor. Tritium 176-178 adrenoceptor beta 3 Rattus norvegicus 214-240 8078497-10 1994 The affinity of [3H]SB 206606 is 76 times higher for the beta 3-adrenergic receptor than for the beta 1/beta 2-adrenergic receptors, thus allowing, under controlled conditions, measurement of interactions only with the beta 3-adrenergic receptor in complex membrane systems. Tritium 17-19 adrenoceptor beta 3 Rattus norvegicus 57-83 8078497-10 1994 The affinity of [3H]SB 206606 is 76 times higher for the beta 3-adrenergic receptor than for the beta 1/beta 2-adrenergic receptors, thus allowing, under controlled conditions, measurement of interactions only with the beta 3-adrenergic receptor in complex membrane systems. Tritium 17-19 adrenoceptor beta 3 Rattus norvegicus 219-245 8032238-2 1994 Sea squirt antigen stimulated normal mouse spleen cells to proliferate, as detected by [3H]-TdR incorporation, in a dose-dependent manner. Tritium 88-90 sepia Mus musculus 0-3 7518514-6 1994 ABT 418 was a potent inhibitor of [3H]-cytisine binding to nAChR in rat brain (Ki = 3 nM) but was inactive (Ki > 10,000 nM) in 37 other receptor/neurotransmitter-uptake/enzyme/transduction system binding assays, including those for alpha-bungarotoxin, muscarinic and 5-hydroxytryptamine3 receptors. Tritium 35-37 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 59-64 7970840-6 1994 With [3H]insP3 as a ligand, Scatchard (Rosenthal) analyses of the concentration-dependent saturation curves showed that the densities (Bmax) of insP3 receptors (insP3R) were significantly increased throughout the time course of adjuvant-induced peripheral inflammation. Tritium 6-8 inositol 1,4,5-trisphosphate receptor, type 1 Rattus norvegicus 161-167 8199201-10 1994 The MP medium effect on the uptake of liposomal [3H]CLE was interfered with by heparin, anti-LPL antibody or heparinase, while these treatments did not affect [3H]cholesterol esterification in the presence of beta VLDL. Tritium 49-51 lipoprotein lipase Mus musculus 93-96 8163582-2 1994 At 30-min of reperfusion after 20 min of forebrain ischemia, [3H]nimodipine binding was significantly increased in striatum, CA3 and CA4, and dentate relative to binding in sham-operated rats, suggesting that VSCCs were responding to ischemic depolarization. Tritium 62-64 carbonic anhydrase 3 Rattus norvegicus 125-128 8040353-3 1994 A radioimmunoassay for the determination of FCE 26073 in human plasma and urine was developed using this antiserum and tritium labeled turosteride. Tritium 119-126 ferrochelatase Homo sapiens 44-47 7916385-3 1994 The addition of Z-300 significantly increased [3H]-labeled mucin in the corpus region. Tritium 47-49 solute carrier family 13 member 2 Rattus norvegicus 59-64 8170952-5 1994 In isolated rat islets, Reg protein (18-180 nM: 0.3-3 micrograms/ml) significantly increased [3H]thymidine incorporation into the nuclei of beta cells. Tritium 94-96 regenerating family member 1 alpha Rattus norvegicus 24-27 8070898-2 1994 SM-15178 inhibited [3H]LTB4 binding to its receptors on human neutrophils (IC50 = 0.30 microM). Tritium 20-22 prostaglandin reductase 1 Cavia porcellus 23-27 8065494-6 1994 Although (+)-MK801-NCS decreased [3H](+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d; ccyclohepten-5,10-imine maleate ([3H](+)-MK801) binding to site 1, it had no effect on [3H]TCP binding to site 1. Tritium 34-36 secretoglobin, family 1B, member 27 Mus musculus 171-174 8137253-3 1994 The uptake of [3H]SarCNU was found to be temperature dependent in SK-MG-1 and SKI-1, but less so in SKI-1. Tritium 15-17 membrane bound transcription factor peptidase, site 1 Homo sapiens 78-83 8137253-3 1994 The uptake of [3H]SarCNU was found to be temperature dependent in SK-MG-1 and SKI-1, but less so in SKI-1. Tritium 15-17 membrane bound transcription factor peptidase, site 1 Homo sapiens 100-105 8137253-4 1994 At 37 degrees C, uptake of 50 microM [3H]SarCNU was linear up to 4 s in both cell lines, with uptake being significantly faster in SK-MG-1 than in SKI-1 under initial rate conditions. Tritium 38-40 membrane bound transcription factor peptidase, site 1 Homo sapiens 147-152 8200423-0 1994 SR 48692 inhibits neurotensin-induced [3H]dopamine release in rat striatal slices and mesencephalic cultures. Tritium 39-41 neurotensin Rattus norvegicus 18-29 8200423-2 1994 SR 48692 (100 nM) also suppressed the neurotensin (10 nM)-induced increase (47%) in K(+)-evoked [3H]dopamine release in primary cultures of fetal rat mesencephalic cells. Tritium 97-99 neurotensin Rattus norvegicus 38-49 8206517-5 1994 A significant reduction in the level of [3H]thymidine incorporation by spleen cells from chronically infected mice stimulated with a T. cruzi antigen preparation ensued when TIF was added to the cultures. Tritium 41-43 TYRO3 protein tyrosine kinase 3 Mus musculus 174-177 8297391-4 1994 The determination of its molecular mass (about 70 kDa) and its pH range for optimum activity (neutral pH) as well as the results obtained with various inhibitors indicate that this [3H]Val-Leu-Met releasing enzyme resembles endopeptidase 24.15: a thiol-dependent zinc metallopeptidase. Tritium 182-184 thimet oligopeptidase 1 Sus scrofa 224-243 7911742-4 1994 The accumulation of [3H]-VP-16 observed in SKOV3/VP cells was about half that seen in SKOV3 cells, and the accumulation of Adriamycin by this resistant cell line was also lower than that of its parent. Tritium 21-23 host cell factor C1 Homo sapiens 25-30 8130931-7 1994 In the hippocampus, alpha,beta-methylene ADP (100 microM) increased the evoked release of tritium, and adenosine deaminase (2 U/ml) produced an even greater increase; when adenosine deaminase was added in the presence of alpha,beta-methylene ADP, adenosine deaminase still increased the evoked release of tritium. Tritium 305-312 adenosine deaminase Rattus norvegicus 103-122 8130931-7 1994 In the hippocampus, alpha,beta-methylene ADP (100 microM) increased the evoked release of tritium, and adenosine deaminase (2 U/ml) produced an even greater increase; when adenosine deaminase was added in the presence of alpha,beta-methylene ADP, adenosine deaminase still increased the evoked release of tritium. Tritium 305-312 adenosine deaminase Rattus norvegicus 172-191 8130931-7 1994 In the hippocampus, alpha,beta-methylene ADP (100 microM) increased the evoked release of tritium, and adenosine deaminase (2 U/ml) produced an even greater increase; when adenosine deaminase was added in the presence of alpha,beta-methylene ADP, adenosine deaminase still increased the evoked release of tritium. Tritium 305-312 adenosine deaminase Rattus norvegicus 172-191 8130931-8 1994 In the cerebral cortex, DPCPX (20 nM) and adenosine deaminase (2 U/ml) increased the evoked tritium release by a similar magnitude, but the effect of adenosine deaminase was smaller than in the hippocampus. Tritium 92-99 adenosine deaminase Rattus norvegicus 42-61 8263044-1 1994 Quantitative receptor autoradiography was used to evaluate the density of high-affinity binding sites for the "peripheral-type" benzodiazepine receptor (PTBR) ligand [3H]PK11195 in brain regions of the rat at different stages of pyrithiamine-induced thiamine deficiency encephalopathy, an experimental model of the Wernicke-Korsakoff syndrome (WKS). Tritium 167-169 translocator protein Rattus norvegicus 110-151 8263044-1 1994 Quantitative receptor autoradiography was used to evaluate the density of high-affinity binding sites for the "peripheral-type" benzodiazepine receptor (PTBR) ligand [3H]PK11195 in brain regions of the rat at different stages of pyrithiamine-induced thiamine deficiency encephalopathy, an experimental model of the Wernicke-Korsakoff syndrome (WKS). Tritium 167-169 translocator protein Rattus norvegicus 153-157 7714547-3 1994 PBR Bmax was assessed by [3H]PK-11195 in vitro binding in surgical specimens and GUR was measured by Positron Emission Tomography with [18F]2-Fluorodeoxyglucose before the surgery. Tritium 26-28 translocator protein Homo sapiens 0-3 7873123-3 1994 In middle-aged gerbils (16 months old), [3H]PDBu binding was significantly reduced in the hippocampal CA1 sector, thalamus, substantia nigra, and cerebellum, compared with young animals (1 month old). Tritium 41-43 carbonic anhydrase 1 Homo sapiens 102-105 7873123-4 1994 [3H]IP3 binding revealed significant elevations in the nucleus accumbens, hippocampal CA1 sector, dentate gyrus, and a significant reduction in cerebellum of middle-aged gerbils. Tritium 1-3 carbonic anhydrase 1 Homo sapiens 86-89 8145303-2 1994 A hetrazepine, BN-50730, is shown here to displace [3H]PAF-specific binding from microsomal, but not from synaptosomal membranes, indicating selectivity for a high affinity intracellular binding site. Tritium 52-54 PCNA clamp associated factor Rattus norvegicus 55-58 7510997-4 1994 Immunopurification from cytosol of [3H]steroid-labeled tungstate-stabilized PR with anti-PR immunoadsorbent yielded "9S"-PR species in which hsp90, hsp70 and p59/HBI were present. Tritium 36-38 heat shock protein family A (Hsp70) member 4 Homo sapiens 148-153 7510997-4 1994 Immunopurification from cytosol of [3H]steroid-labeled tungstate-stabilized PR with anti-PR immunoadsorbent yielded "9S"-PR species in which hsp90, hsp70 and p59/HBI were present. Tritium 36-38 HLA complex P5B Homo sapiens 158-161 7991790-2 1994 Proteins in the 20-kDa range, conceptus-secreted retinol-binding protein (RBP), bound both [3H]retinol and [3H]RA specifically. Tritium 92-94 retinol binding protein 4 Sus scrofa 49-72 7991790-2 1994 Proteins in the 20-kDa range, conceptus-secreted retinol-binding protein (RBP), bound both [3H]retinol and [3H]RA specifically. Tritium 92-94 retinol binding protein 4 Sus scrofa 74-77 7991790-2 1994 Proteins in the 20-kDa range, conceptus-secreted retinol-binding protein (RBP), bound both [3H]retinol and [3H]RA specifically. Tritium 108-110 retinol binding protein 4 Sus scrofa 49-72 7991790-2 1994 Proteins in the 20-kDa range, conceptus-secreted retinol-binding protein (RBP), bound both [3H]retinol and [3H]RA specifically. Tritium 108-110 retinol binding protein 4 Sus scrofa 74-77 7991790-3 1994 Cross-competition experiments indicate that [3H]RA was completely displaced with excess cold retinol; however, excess cold RA did not completely displace [3H]retinol, suggesting that conceptus RBP has greater affinity for retinol than RA. Tritium 45-47 retinol binding protein 4 Sus scrofa 193-196 7505012-2 1993 First, we established an in vivo assay using [3H]HA to measure the binding of HA to mouse T lymphoma cells, and found that the binding of [3H]HA to these cells is readily inhibited by the addition of anti-GP85(CD44) antibody suggesting that GP85(CD44) is the HA receptor. Tritium 139-141 CD44 antigen Mus musculus 210-214 7505012-2 1993 First, we established an in vivo assay using [3H]HA to measure the binding of HA to mouse T lymphoma cells, and found that the binding of [3H]HA to these cells is readily inhibited by the addition of anti-GP85(CD44) antibody suggesting that GP85(CD44) is the HA receptor. Tritium 139-141 CD44 antigen Mus musculus 246-250 8307574-9 1993 Expression of PBR cDNA in pCMV5 vectors transfected into COS-1 cells resulted in increased binding of [3H]PK11195, but expression of PBR-S did not. Tritium 103-105 translocator protein Homo sapiens 14-17 8240280-3 1993 Rat1 fibroblasts overexpressing HIR-A or -B (rat1-HIR cells) were labelled with [3H]glucosamine, [3H]mannose or myo-[3H]inositol. Tritium 81-83 histone cell cycle regulator Rattus norvegicus 32-43 8114757-3 1993 In K2 mice with 30-fold testicular ODC overexpression, [3H]thymidine incorporation at stages I-VI of the cycle of the seminiferous epithelium was significantly above the control level. Tritium 56-58 ornithine decarboxylase, structural 1 Mus musculus 35-38 8114757-6 1993 In K15 mice, [3H]thymidine incorporation of stage VIII tubule segments was decreased, suggesting that excess amounts of putrescine selectively inhibit meiotic DNA synthesis. Tritium 14-16 keratin 15 Mus musculus 3-6 8010061-1 1993 O6-Methylguanine DNA methyltransferase (O6-MT) activity and cellular sensitivity to nitrosourea drugs of 10 kinds of tumor cell strains derived from Chinese patients were measured by 3H radioactivity and colony-forming ability, respectively. Tritium 183-185 O-6-methylguanine-DNA methyltransferase Homo sapiens 40-45 8215408-2 1993 The relative affinities of these congeners for the aryl hydrocarbon (Ah) receptor were determined using rat hepatic cytosol and 2,3,7,8-[3H]tetrachlorodibenzo-p-dioxin ([3H]TCDD) as the radioligand. Tritium 137-139 aryl hydrocarbon receptor Rattus norvegicus 51-81 8221112-1 1993 The densities of [3H]1,3-di-o-tolylguanidine ([3H]DTG) binding to sigma binding sites in the CA1 stratum pyramidale region in 7 hippocampi affected by Alzheimer"s disease, were compared with densities in 7 normal hippocampi. Tritium 18-20 carbonic anhydrase 1 Homo sapiens 93-96 8395564-5 1993 In an age-selected group (range, 10-89 years), the density of monoamine oxidase (MAO)-B sites labeled by [3H]Ro 19-6327 (lazabemide) also showed a positive correlation with age (r = 0.80; p < 0.005). Tritium 106-108 monoamine oxidase B Homo sapiens 62-87 8395564-8 1993 In the human frontal cortex, idazoxan displayed very low affinity (Ki = 89 microM) against the binding of [3H]Ro 19-6327 to MAO-B, which discounted a direct interaction of [3H]idazoxan with the active center of the enzyme and indicated that the I2-imidazoline site cannot be identified with MAO-B. Tritium 107-109 monoamine oxidase B Homo sapiens 124-129 8401931-0 1993 [3H]-5-carboxamidotryptamine labels 5-HT1D binding sites in bovine substantia nigra. Tritium 1-3 5-hydroxytryptamine receptor 1D Homo sapiens 36-42 8401931-2 1993 [3H]-5-hydroxytryptamine (5-HT) has been shown to radiolabel at least five types of 5-HT binding sites in mammalian brain tissue, 5-HT1A, 5-HT1C, 5-HT1D and 5-HT1D and 5-HT1E (Frazer et al., 1990). Tritium 1-3 5-hydroxytryptamine receptor 2C Homo sapiens 138-144 8401931-2 1993 [3H]-5-hydroxytryptamine (5-HT) has been shown to radiolabel at least five types of 5-HT binding sites in mammalian brain tissue, 5-HT1A, 5-HT1C, 5-HT1D and 5-HT1D and 5-HT1E (Frazer et al., 1990). Tritium 1-3 5-hydroxytryptamine receptor 1D Homo sapiens 146-152 8401931-2 1993 [3H]-5-hydroxytryptamine (5-HT) has been shown to radiolabel at least five types of 5-HT binding sites in mammalian brain tissue, 5-HT1A, 5-HT1C, 5-HT1D and 5-HT1D and 5-HT1E (Frazer et al., 1990). Tritium 1-3 5-hydroxytryptamine receptor 1D Homo sapiens 157-163 8401931-6 1993 [3H]-5-CT binding to 5-HT1D sites in bovine substantia nigra was rapid, reversible and saturable, displaying high affinity (Kd = 0.38 +/- 0.04 nM) and low non-specific binding (> 90% specific binding). Tritium 1-3 5-hydroxytryptamine receptor 1D Homo sapiens 21-27 8323282-6 1993 Elastin and poly-L-lysine were labeled by reductive methylation of amino groups with [3H]HCHO prior to treatment with NO2 in aqueous solutions at physiological pH. Tritium 86-88 elastin Homo sapiens 0-7 8323282-7 1993 NO2 exposure of elastin resulted in the solubilization of 84% of the associated radioactivity of which 79% was identified as [3H]methyllysine by amino acid analysis. Tritium 126-128 elastin Homo sapiens 16-23 8374053-4 1993 With microsomes, 6.5 mumol of [3H]6-FBP/mol of DNA-P and 10 mumol of [14C]6-CH3BP/mol of DNA-P were bound vs 15 mumol of [3H]BP. Tritium 31-33 far upstream element binding protein 1 Rattus norvegicus 36-39 8394771-4 1993 The results showed an age-related decrease in the density of [3H]-KA binding in several regions of the hippocampal formation (CA3, CA1, hilus) and within related cortical areas (subicular complex, entorhinal cortex, perirhinal cortex). Tritium 62-64 carbonic anhydrase 3 Rattus norvegicus 126-129 8105069-3 1993 [3H]cAMP binding in the stratum oriens and pyramidale of the hippocampal CA1 sector significantly decreased in the early post-ischaemic stage and showed severe reduction 7 days and 1 month after recirculation. Tritium 1-3 carbonic anhydrase 1 Homo sapiens 73-76 8323557-4 1993 We report that infusion of either caerulein or CCK-JMV-180 into rats increases [3H]-thymidine incorporation into pancreatic DNA and causes the pancreatic weight as well as content of DNA, RNA, and protein to increase. Tritium 80-82 cholecystokinin Rattus norvegicus 47-50 8504756-2 1993 This anti-serum was shown to recognize the human GLUT3 protein in Chinese hamster ovary cells transfected with GLUT3 cDNA and to immunoprecipitate an authentic glucose transport protein in brain and testis membranes, as assessed by glucose-inhibitable photolabeling with [3H] cytochalasin-B. Tritium 272-274 solute carrier family 2 member 3 Homo sapiens 49-54 8097750-9 1993 Anti-CD44 mAb-triggered proliferation ([3H]thymidine incorporation) and cytotoxicity are blocked by the protein tyrosine kinase inhibitor, genestein. Tritium 40-42 CD44 molecule (Indian blood group) Homo sapiens 5-9 8361637-4 1993 (4) Chronic stress: (a) reduced the endogenous levels of GABA in the frontal cerebral cortex (51%), hypothalamus (26%) and olfactory bulbs (15%); (b) decreased GAD activity in the corpus striatum (32%) and olfactory bulbs (34%); (c) decreased neuronal uptake of [3H]GABA in the hypothalamus (83%). Tritium 263-265 glutamate-ammonia ligase Rattus norvegicus 160-163 8387652-3 1993 Autoradiographic analysis of second messenger systems showed that 3-min ischemia caused a significant reduction in [3H]inositol-1,4,5-trisphosphate binding in the hippocampal CA1 sector, whereas the alteration in [3H]phorbol 12,13-dibutyrate, [3H]forskolin and [3H] cyclic-AMP bindings was not found in this region. Tritium 116-118 carbonic anhydrase 1 Homo sapiens 175-178 8480329-14 1993 Additionally, linuron competed with [3H]testosterone for binding to the androgen receptor. Tritium 37-39 androgen receptor Rattus norvegicus 72-89 7682062-0 1993 Characterization of the binding sites of [3H]SR 48968, a potent nonpeptide radioligand antagonist of the neurokinin-2 receptor. Tritium 42-44 tachykinin receptor 2 Homo sapiens 105-126 7682062-1 1993 [3H]SR 48968, a radiolabeled nonpeptide antagonist of NK-2 receptor, has been tested in ligand-receptor binding assays using rat duodenum, hamster urinary bladder and guinea pig ileum membranes. Tritium 1-3 tachykinin receptor 2 Rattus norvegicus 54-67 7682062-7 1993 These data show that [3H]SR 48968 potently binds to NK-2 receptor and therefore is a useful tool to study NK-2 receptor. Tritium 22-24 tachykinin receptor 2 Homo sapiens 52-65 7682062-7 1993 These data show that [3H]SR 48968 potently binds to NK-2 receptor and therefore is a useful tool to study NK-2 receptor. Tritium 22-24 tachykinin receptor 2 Homo sapiens 106-119 7682074-6 1993 The same rapid egress of [3H]MTX and [3H]MTX+G1 seen following pulse loading was also documented in cells grown for 3 hr in the presence of nonradioactive MTX to normalize conditions with respect to the intracellular accumulation of [3H]MTX polyglutamates seen during exposure of cells to [3H]MTX during growth. Tritium 38-40 metaxin 1 Mus musculus 41-44 7682074-6 1993 The same rapid egress of [3H]MTX and [3H]MTX+G1 seen following pulse loading was also documented in cells grown for 3 hr in the presence of nonradioactive MTX to normalize conditions with respect to the intracellular accumulation of [3H]MTX polyglutamates seen during exposure of cells to [3H]MTX during growth. Tritium 38-40 metaxin 1 Mus musculus 41-44 7682074-6 1993 The same rapid egress of [3H]MTX and [3H]MTX+G1 seen following pulse loading was also documented in cells grown for 3 hr in the presence of nonradioactive MTX to normalize conditions with respect to the intracellular accumulation of [3H]MTX polyglutamates seen during exposure of cells to [3H]MTX during growth. Tritium 38-40 metaxin 1 Mus musculus 41-44 7682074-6 1993 The same rapid egress of [3H]MTX and [3H]MTX+G1 seen following pulse loading was also documented in cells grown for 3 hr in the presence of nonradioactive MTX to normalize conditions with respect to the intracellular accumulation of [3H]MTX polyglutamates seen during exposure of cells to [3H]MTX during growth. Tritium 38-40 metaxin 1 Mus musculus 41-44 7682074-6 1993 The same rapid egress of [3H]MTX and [3H]MTX+G1 seen following pulse loading was also documented in cells grown for 3 hr in the presence of nonradioactive MTX to normalize conditions with respect to the intracellular accumulation of [3H]MTX polyglutamates seen during exposure of cells to [3H]MTX during growth. Tritium 38-40 metaxin 1 Mus musculus 41-44 7682074-6 1993 The same rapid egress of [3H]MTX and [3H]MTX+G1 seen following pulse loading was also documented in cells grown for 3 hr in the presence of nonradioactive MTX to normalize conditions with respect to the intracellular accumulation of [3H]MTX polyglutamates seen during exposure of cells to [3H]MTX during growth. Tritium 38-40 metaxin 1 Mus musculus 41-44 7682074-6 1993 The same rapid egress of [3H]MTX and [3H]MTX+G1 seen following pulse loading was also documented in cells grown for 3 hr in the presence of nonradioactive MTX to normalize conditions with respect to the intracellular accumulation of [3H]MTX polyglutamates seen during exposure of cells to [3H]MTX during growth. Tritium 38-40 metaxin 1 Mus musculus 41-44 7682074-6 1993 The same rapid egress of [3H]MTX and [3H]MTX+G1 seen following pulse loading was also documented in cells grown for 3 hr in the presence of nonradioactive MTX to normalize conditions with respect to the intracellular accumulation of [3H]MTX polyglutamates seen during exposure of cells to [3H]MTX during growth. Tritium 38-40 metaxin 1 Mus musculus 41-44 7682074-8 1993 ATP-dependent efflux of [3H]MTX and [3H]MTX+G1 exhibited values for Km of 46-50 microM and values for Vmax of 102-106 pmol/min/mg protein. Tritium 25-27 metaxin 1 Mus musculus 28-31 7682074-8 1993 ATP-dependent efflux of [3H]MTX and [3H]MTX+G1 exhibited values for Km of 46-50 microM and values for Vmax of 102-106 pmol/min/mg protein. Tritium 37-39 metaxin 1 Mus musculus 40-43 7682074-9 1993 As competitive inhibitors of [3H]MTX and [3H]MTX+G1 efflux, MTX+G1 and MTX, respectively, exhibited Ki values of 43-47 microM, that is, Km approximately Ki for both permeants. Tritium 30-32 metaxin 1 Mus musculus 33-36 7682074-9 1993 As competitive inhibitors of [3H]MTX and [3H]MTX+G1 efflux, MTX+G1 and MTX, respectively, exhibited Ki values of 43-47 microM, that is, Km approximately Ki for both permeants. Tritium 30-32 metaxin 1 Mus musculus 60-74 7682074-9 1993 As competitive inhibitors of [3H]MTX and [3H]MTX+G1 efflux, MTX+G1 and MTX, respectively, exhibited Ki values of 43-47 microM, that is, Km approximately Ki for both permeants. Tritium 42-44 metaxin 1 Mus musculus 45-48 7682074-9 1993 As competitive inhibitors of [3H]MTX and [3H]MTX+G1 efflux, MTX+G1 and MTX, respectively, exhibited Ki values of 43-47 microM, that is, Km approximately Ki for both permeants. Tritium 42-44 metaxin 1 Mus musculus 60-74 7682074-10 1993 Also, values for Ki of 45-48 microM were obtained with MTX+G2 and MTX+G4 as competitive inhibitors of [3H]MTX efflux. Tritium 103-105 metaxin 1 Mus musculus 55-58 7682074-10 1993 Also, values for Ki of 45-48 microM were obtained with MTX+G2 and MTX+G4 as competitive inhibitors of [3H]MTX efflux. Tritium 103-105 metaxin 1 Mus musculus 66-69 7682074-10 1993 Also, values for Ki of 45-48 microM were obtained with MTX+G2 and MTX+G4 as competitive inhibitors of [3H]MTX efflux. Tritium 103-105 metaxin 1 Mus musculus 66-69 8384853-6 1993 The ability of 13C or DIM to cause in vitro transformation of the Ah receptor to a form able to bind to the dioxin-responsive element-3 (DRE3) was compared to that of TCDD and shown to parallel their abilities to compete for binding of [3H]TCDD to the Ah receptor. Tritium 237-239 aryl hydrocarbon receptor Rattus norvegicus 66-77 8441394-7 1993 PC-PLC addition to CSF-1-dependent BAC1.2F5 clones that constitutively express c-myc increased [3H]thymidine incorporation to 86% of the level evoked by CSF-1 and supported slow growth in the absence of CSF-1. Tritium 96-98 colony stimulating factor 1 (macrophage) Mus musculus 19-24 8429387-4 1993 [3H]ROL added directly to the medium was rapidly taken up by HKc, and maximal accumulation of [3H]ROL occurred by 3 h. In contrast, [3H]ROL delivered bound to RBP was taken up very slowly but at a linear rate for at least 72 h. Several experimental approaches indicated that ROL uptake from RBP was not mediated by a cell surface receptor for RBP. Tritium 1-3 retinol binding protein 4 Homo sapiens 159-162 8423963-3 1993 Platelet-activating factor acetylhydrolase activity was assayed in serum and peritoneal fluid by the release of 3H-acetate from [3H]-platelet-activating factor during a 15-minute incubation. Tritium 112-114 phospholipase A2 group VII Homo sapiens 0-42 8469389-1 1993 Developmental profiles of [3H]mesulergine-labelled central 5-HT1c binding sites differed regionally in the rat during the first 5 weeks postnatal. Tritium 27-29 5-hydroxytryptamine receptor 2C Rattus norvegicus 59-65 8440133-0 1993 Regional sublocalization of the human thyroid peroxidase gene (TPO) by tritium and fluorescence in situ hybridization to chromosome 2p25-->p24. Tritium 71-78 thyroid peroxidase Homo sapiens 38-56 8440133-0 1993 Regional sublocalization of the human thyroid peroxidase gene (TPO) by tritium and fluorescence in situ hybridization to chromosome 2p25-->p24. Tritium 71-78 thyroid peroxidase Homo sapiens 63-66 8383782-6 1993 HGF was additive with EGF in stimulating [3H] thymidine incorporation. Tritium 42-44 hepatocyte growth factor Oryctolagus cuniculus 0-3 8383782-12 1993 Preincubation of rabbit proximal tubule cells with the tyrosine kinase inhibitor, genistein (50 microM), prevented HGF-stimulation of [3H] thymidine incorporation. Tritium 135-137 hepatocyte growth factor Oryctolagus cuniculus 115-118 8280369-4 1993 In whole cells, a GRP analogue bound to these receptors and stimulated rapid transfer of tritium from the tritiated lipid inositol pool to inositol triphosphates. Tritium 89-96 gastrin releasing peptide Homo sapiens 18-21 8383818-8 1993 Reductions in [3H]MK-801 binding in CA1 were observed four days after ischemic insults when CA1 neuronal destruction was seen. Tritium 15-17 carbonic anhydrase 1 Homo sapiens 36-39 8383818-8 1993 Reductions in [3H]MK-801 binding in CA1 were observed four days after ischemic insults when CA1 neuronal destruction was seen. Tritium 15-17 carbonic anhydrase 1 Homo sapiens 92-95 8383818-9 1993 After one month, approximately 50% of [3H]MK-801 binding was lost in CA1 in both groups. Tritium 39-41 carbonic anhydrase 1 Homo sapiens 69-72 1282920-15 1992 The adult distribution of beta 2 and beta 3 cRNA probes showed good spatial correspondence with the known GABAA receptor beta subunit markers, [3H]-muscimol and the mAb 62-3G1 antibody, each being present within the granule cell layer. Tritium 144-146 hemoglobin, beta adult minor chain Mus musculus 26-43 1360981-2 1992 P-gp has been localized to the apical plasma membrane of the bile canaliculus where it has been shown to transport [3H]daunomycin. Tritium 116-118 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 0-4 1360981-3 1992 In this study, we investigated whether alterations in membrane lipid fluidity of canalicular membrane vesicles (CMV) could modulate the P-gp-mediated accumulation of [3H]daunomycin and [3H]vinblastine. Tritium 167-169 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 136-140 1360981-3 1992 In this study, we investigated whether alterations in membrane lipid fluidity of canalicular membrane vesicles (CMV) could modulate the P-gp-mediated accumulation of [3H]daunomycin and [3H]vinblastine. Tritium 186-188 ATP-binding cassette, subfamily B (MDR/TAP), member 1B Rattus norvegicus 136-140 1446793-7 1992 The time course of ET-1 stimulation as measured by [3H]glycerol labeling, and IP3 level showed a 98% increase in [3H]DAG at 10 min and a fourfold increase for IP3, respectively. Tritium 52-54 endothelin 1 Bos taurus 19-23 1446793-7 1992 The time course of ET-1 stimulation as measured by [3H]glycerol labeling, and IP3 level showed a 98% increase in [3H]DAG at 10 min and a fourfold increase for IP3, respectively. Tritium 114-116 endothelin 1 Bos taurus 19-23 1449538-2 1992 When the LMM-PLA2 and HMM-PLA2 enzymes were examined for hydrolysis against [3H]AA Escherichia coli in an ethyleneglycol-bis(beta-aminoethyl ether) N,N,N",N"-tetraacetic acid (EGTA)-free buffer system, neither enzyme demonstrated activity below 10 microM free Ca2+. Tritium 77-79 phospholipase A2 group IIA Homo sapiens 13-17 1449538-2 1992 When the LMM-PLA2 and HMM-PLA2 enzymes were examined for hydrolysis against [3H]AA Escherichia coli in an ethyleneglycol-bis(beta-aminoethyl ether) N,N,N",N"-tetraacetic acid (EGTA)-free buffer system, neither enzyme demonstrated activity below 10 microM free Ca2+. Tritium 77-79 phospholipase A2 group IIA Homo sapiens 26-30 1281606-6 1992 [3H]Thymidine incorporation studies showed that SCF increased the proliferation of MNC precursors. Tritium 1-3 KIT ligand Homo sapiens 48-51 1357099-0 1992 Stimulation of delta-opioid receptors reduces the in vivo binding of the cholecystokinin (CCK)-B-selective agonist [3H]pBC 264: evidence for a physiological regulation of CCKergic systems by endogenous enkephalins. Tritium 116-119 cholecystokinin Mus musculus 73-88 1357099-0 1992 Stimulation of delta-opioid receptors reduces the in vivo binding of the cholecystokinin (CCK)-B-selective agonist [3H]pBC 264: evidence for a physiological regulation of CCKergic systems by endogenous enkephalins. Tritium 116-119 cholecystokinin Mus musculus 90-93 1402922-6 1992 Although a protein kinase C (PKC) inhibitor, staurosporine (5 microM), blocked PMA-induced [3H]PEt formation by 77%, it had no effect on the CCh-induced formation. Tritium 92-94 protein kinase C, gamma Rattus norvegicus 29-32 1282220-2 1992 Activators of PKC, such as phorbol 12-myristate 13-acetate (PMA), mezerein and oleoyl acetylglycerol produced a concentration-dependent potentiation of K(+)-induced release of [3H]5-hydroxytryptamine ([3H]5-HT). Tritium 177-179 protein kinase C, gamma Rattus norvegicus 14-17 1282220-7 1992 Similarly, the PKC inhibitors, polymyxin B and staurosporine, blocked effects of both PMA and Bay K 8644 on K(+)-stimulated release of [3H]5-HT. Tritium 136-138 protein kinase C, gamma Rattus norvegicus 15-18 1461361-1 1992 This study has shown that neurotensin (NT) increases the electrically stimulated release of [3H]DA to a similar extent in all but the extreme caudolateral area of the rat nucleus accumbens and appears to modulate DA release equally in the medial and lateral zones of this brain area. Tritium 93-95 neurotensin Rattus norvegicus 26-37 1362264-4 1992 A significant (p < 0.02) inhibitory effect of somatostatin (1 nM to 10 microM) on 3H-thymidine DNA incorporation was observed in cultured chondrocytes from fetuses of all gestational ages studied (12-40 wk), with no significant differences among fetal ages. Tritium 85-87 somatostatin Homo sapiens 49-61 1356434-6 1992 Following hydrolysis of [3H]ATP, a relatively stable [3H]ADP.Hsc70 complex was formed. Tritium 25-27 heat shock protein family A (Hsp70) member 8 Homo sapiens 61-66 1356434-7 1992 Release of [3H]ADP from Hsc70 was most efficient in the presence of other nucleotides such as ADP or ATP, suggesting that ADP release occurs as an ADP/ATP exchange reaction. Tritium 12-14 heat shock protein family A (Hsp70) member 8 Homo sapiens 24-29 1327423-3 1992 Two-minute forebrain ischemia, which produced no neuronal damage, resulted in no alterations in binding except for a slight reduction in [3H]QNB binding in the CA1 subfield. Tritium 138-140 carbonic anhydrase 1 Homo sapiens 160-163 1388045-4 1992 Substrate, ([3H]progesterone) and product ([3H]5 alpha-DHP), were separated by reverse phase HPLC. Tritium 44-46 dihydropyrimidinase Rattus norvegicus 55-58 1321849-7 1992 The activity of acetyl CoA:2-lyso-PAF acetyltransferase was transiently increased in porin-stimulated PMN and the [3H]acetyl group was incorporated in the synthetized PAF after cell preincubation with [3H]acetyl CoA. Tritium 115-117 lysophosphatidylcholine acyltransferase 1 Homo sapiens 29-55 1321849-7 1992 The activity of acetyl CoA:2-lyso-PAF acetyltransferase was transiently increased in porin-stimulated PMN and the [3H]acetyl group was incorporated in the synthetized PAF after cell preincubation with [3H]acetyl CoA. Tritium 202-204 lysophosphatidylcholine acyltransferase 1 Homo sapiens 29-55 1504012-11 1992 (4) Comparative binding studies disclosed that the apparent Kd-values (86.5 +/- 7.3 and 77.0 +/- 14.3 nM, respectively) of the [3H]B interactions with CBG and GCR did not differ (P greater than 0.3). Tritium 128-130 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 159-162 1322664-12 1992 Although the difference between values for displacing potency and ability to inhibit cell multiplication cannot be explained as yet, it is interesting that all PBR-ligands followed the same sequence in displacing [3H]Pk 11195 and inhibiting cell multiplication and that central type ligands were ineffective in both assays. Tritium 214-216 translocator protein Homo sapiens 160-163 1643116-6 1992 On the other hand, the results indicated a rapid accumulation of intracellular free [3H]arachidonic acid that paralleled the appearance of both [3H]glycerol-labeled lysophosphatidylcholine and [3H]glycerol-labeled lysophosphatidylinositol, the by-products of phospholipase A2 action on phosphatidylcholine and phosphatidylinositol, respectively. Tritium 85-87 phospholipase A2, group IB, pancreas Mus musculus 259-275 1326278-6 1992 The PBR ligand [3H]PK 11195 had high affinity for the expressed receptor in COS-1 cells, but the affinities of a pair of isoquinoline propanamide enantiomers differed remarkably in expressed and endogenous human PBR. Tritium 16-18 translocator protein Homo sapiens 4-7 1378162-2 1992 In our experiments human recombinant SCF stimulated short-term proliferation of accessory cell-depleted acute myeloid leukemia (AML) cells in 13/14 cases, as determined by 3H-thymidine (3H-TdR) incorporation and cell counts. Tritium 172-174 KIT ligand Homo sapiens 37-40 1378162-5 1992 However, SCF potentiated the stimulatory effect of GM-CSF, G-CSF, and IL-3 on both 3H-TdR incorporation and colony formation. Tritium 83-85 KIT ligand Homo sapiens 9-12 1407551-4 1992 When the data were averaged and the means for Alzheimer"s disease and control group compared, a 34% decrease (P less than 0.01) in [3H]MK-801 binding was identified in the CA1 stratum pyramidale and a smaller decrease was found in the dentate gyrus molecular layer, parahippocampal gyrus and subiculum. Tritium 132-134 carbonic anhydrase 1 Homo sapiens 172-175 1627622-6 1992 Homogenates of MEPM cells which had been prelabeled with [3H]lyso-PAF and [14C]arachidonic acid produced 14C-fatty acid, [3H]lyso-PC, and [3H]alkylglycerol when incubated at selected values of pH and concentrations of calcium. Tritium 58-61 patchy fur Mus musculus 66-69 1375845-6 1992 Proliferation of AA cells cultured in a liquid medium containing SCF together with Epo, IL-3, GM-CSF, and G-CSF approached 70% of the control level, as measured by 3H-thymidine incorporation. Tritium 164-166 KIT ligand Homo sapiens 65-68 1616350-2 1992 Anticardiolipin antibodies at concentrations from 50 to 200 micrograms/ml inhibited the [3H]thymidine incorporation of RBA-1 cells in a dose dependent manner after three days of culture. Tritium 89-91 calcium voltage-gated channel subunit alpha1 A Rattus norvegicus 119-124 1528043-5 1992 Exogenous TGF-alpha (30 nmol/kg, sc every 12 hr) significantly improved [3H]thymidine incorporation (35.6 +/- 8.2 cpm/micrograms DNA, P less than 0.05). Tritium 73-75 transforming growth factor alpha Rattus norvegicus 10-19 1614411-7 1992 The CCK-B antagonist L365,260 was 18-, 30-, and 64-fold less potent than [3H]pBC 264 in guinea pig, mouse, and rat, respectively. Tritium 74-76 cholecystokinin Mus musculus 4-7 1523167-0 1992 Uptake and metabolism of [3H]-Leu-enkephalin following either its intraperitoneal or subcutaneous administration to mice. Tritium 26-29 prodynorphin Mus musculus 30-44 1523167-1 1992 The uptake and metabolism of 30 micrograms/kg [3H]-Leu-enkephalin ([3H]-LE) following either intraperitoneal (IP) or subcutaneous (SC) administration to Swiss Webster mice was examined. Tritium 47-50 prodynorphin Mus musculus 51-65 1523167-1 1992 The uptake and metabolism of 30 micrograms/kg [3H]-Leu-enkephalin ([3H]-LE) following either intraperitoneal (IP) or subcutaneous (SC) administration to Swiss Webster mice was examined. Tritium 47-49 prodynorphin Mus musculus 51-65 1315531-3 1992 Remarkably, [3H]5-hydroxytryptamine binding studies with transfected HeLa cells show that the human 5-HT1B receptor has a pharmacological profile that is markedly different from that of the corresponding rat receptor. Tritium 13-15 5-hydroxytryptamine receptor 1B Rattus norvegicus 100-106 1548463-0 1992 Characterization of [3H]CP-96,501 as a selective radioligand for the serotonin 5-HT1B receptor: binding studies in rat brain membranes. Tritium 21-23 5-hydroxytryptamine receptor 1B Rattus norvegicus 79-85 1560361-5 1992 There was a high correlation between drug affinities for [3H]mesulergine-labeled 5-HT1C sites in spinal cord and those reported in pig cortex (r = 0.94) and choroid plexus (r = 0.93), but poor correlation with 5-HT2 (high or low affinity states) or other 5-HT sites. Tritium 58-60 5-hydroxytryptamine receptor 2C Rattus norvegicus 81-87 1322221-2 1992 [3H]Cyclic AMP binding in the strata pyramidale and lacunosum-moleculare of the hippocampal CA1, the stratum pyramidale of the CA3, and the dentate gyrus decreased transiently in the early postischemic phase but then recovered. Tritium 1-3 carbonic anhydrase 1 Homo sapiens 92-95 1322221-3 1992 However, [3H]cyclic AMP binding in the strata pyramidale and radiatum of the CA1, the granular layer of the dentate gyrus, and the upper layer of the cortex decreased again 7 days after ischemia. Tritium 10-12 carbonic anhydrase 1 Homo sapiens 77-80 1554395-1 1992 Specific binding of polychlorinated biphenyls (PCBs) to rat liver cytosol protein has been detected using the 3H-labeled PCB probe 2,2",4,4",5,5"-hexachlorobiphenyl (6-CB). Tritium 110-112 pyruvate carboxylase Rattus norvegicus 47-50 1542658-3 1992 In cultures of somites containing NC cells at migratory stages, NT-3 promotes a 2- to 8.4-fold increase in the number of NC cells incorporating [3H]thymidine into nuclei and a 1.8- to 4.8-fold increase in NC cell number compared to controls without added factor. Tritium 145-147 neurotrophin 3 Homo sapiens 64-68 1371281-4 1992 In the presence of UDP-[3H]galactose, galactosyltransferase catalyzed the labeling of cytokeratin 8 and 18. beta-Elimination of the [3H]galactose- labeled CK8/18 generated the disaccharide N-acetyllactosaminitol, indicating that cytokeratin 8 and 18 contain single O-linked N-acetylglucosamine residues. Tritium 24-26 keratin 8 Homo sapiens 86-99 1371281-4 1992 In the presence of UDP-[3H]galactose, galactosyltransferase catalyzed the labeling of cytokeratin 8 and 18. beta-Elimination of the [3H]galactose- labeled CK8/18 generated the disaccharide N-acetyllactosaminitol, indicating that cytokeratin 8 and 18 contain single O-linked N-acetylglucosamine residues. Tritium 24-26 keratin 8 Homo sapiens 155-158 1371281-4 1992 In the presence of UDP-[3H]galactose, galactosyltransferase catalyzed the labeling of cytokeratin 8 and 18. beta-Elimination of the [3H]galactose- labeled CK8/18 generated the disaccharide N-acetyllactosaminitol, indicating that cytokeratin 8 and 18 contain single O-linked N-acetylglucosamine residues. Tritium 24-26 keratin 8 Homo sapiens 229-242 1371281-6 1992 Peptide maps of [3H]glucosamine-labeled CK8/18 showed that multiple peptides were labeled with the amino sugar. Tritium 17-19 keratin 8 Homo sapiens 40-43 1347503-0 1992 Radioligand binding studies of the atypical beta 3-adrenergic receptor in rat brown adipose tissue using [3H]CGP 12177. Tritium 106-108 adrenoceptor beta 3 Rattus norvegicus 44-70 1347503-2 1992 The majority of binding sites are of low affinity with a Kd of 31 nM, a value in close agreement with that for the Kd of [3H]CGP 12177 binding to a cloned rat beta 3-adrenergic receptor (AR) expressed in CHO cells (44 nM). Tritium 122-124 adrenoceptor beta 3 Rattus norvegicus 159-185 1729418-5 1992 When endogenous adenosine was degraded by addition of adenosine deaminase, the light-evoked release of radioactivity derived from [3H]choline was significantly increased compared with control values. Tritium 131-133 adenosine deaminase Oryctolagus cuniculus 54-73 1730705-9 1992 In experiments using a two-compartment chamber in which cells in the lower and upper chambers were separated by filter paper yet the cells in both compartments could communicate without direct contact, [3H]FC released into the medium was biologically active and could serve as an efficient substrate for ACAT. Tritium 203-205 sterol O-acyltransferase 2 Mus musculus 304-308 1730582-3 1992 The pyridoxal 5"-phosphate binding lysine in mouse ODC was identified as lysine 69 of the mouse sequence by reduction of the purified holoenzyme form with NaB[3H]4 followed by digestion of the carboxymethylated protein with endoproteinase Lys-C, radioactive peptide mapping using reversed-phase high pressure liquid chromatography and gas-phase peptide sequencing. Tritium 159-161 ornithine decarboxylase, structural 1 Mus musculus 51-54 1727646-3 1992 The high affinity (Kd = 2.5 nM) interaction between [3H]ZK98299 and PR was temperature- and time-dependent, reaching an optimum by 2-3 h at 0 degrees C, and was facilitated by 20 mM Na2MoO4. Tritium 53-55 progesterone receptor Bos taurus 68-70 1605728-4 1992 Exposure of cells to Cd2+ inhibited the liberation of [3H]20:4 in a dose-dependent manner. Tritium 55-57 T-cell surface antigen CD2 Oryctolagus cuniculus 21-24 1605728-5 1992 Liberation of [3H]20:4 from cell lipids was calcium dependent and the inhibitory effect of Cd2+ competed with the stimulatory effect of Ca2+. Tritium 15-17 T-cell surface antigen CD2 Oryctolagus cuniculus 91-94 1483875-3 1992 Furthermore, a monoclonal antibody to the IGF-I receptor suppressed DNA synthesis when determined by a [3H]thymidine incorporation assay. Tritium 104-106 insulin like growth factor 1 receptor Homo sapiens 42-56 1815828-4 1991 Repetitive stimulation of the photoreceptors with light flashes induced about a 47% rise in the rate of formation of [3H]2DG-6P in the glial cells and this effect is probably due to the activation of hexokinase. Tritium 118-120 hexokinase type 2 Apis mellifera 200-210 1667758-4 1991 After galactose induction, the transformed cells expressed a functional hPBR which displayed a Kd for the specific peripheral-type ligand [3H]PK11195 of 9.9 +/- 1.3 nM and a maximal binding capacity of 249,300 +/- 50,400 sites/cell. Tritium 139-141 translocator protein Homo sapiens 72-76 1720089-12 1991 1,25-(OH)2D3 treatment increased secretion of IGFBP-4 up to 14-fold over 24 h. 1,25-(OH)2D3 also increased IGFBP-4 (2.2 kb) transcript levels within 30 min, with the maximal stimulation of 8-fold occurring after 8 h. [3H]Thymidine incorporation into cells was inhibited by 1,25-(OH)2D3 both under basal and serum-stimulated conditions. Tritium 218-220 insulin-like growth factor binding protein 4 Mus musculus 107-114 1744178-5 1991 RSP and PSP stimulated 3H-thymidine incorporation by fibroblasts in a dose-dependent manner. Tritium 23-25 microseminoprotein beta Homo sapiens 8-11 1686053-4 1991 Examination of [3H]pregnenolone metabolism by testes of Tfm mice indicated that progesterone, rather than testosterone, was the major steroid produced. Tritium 16-18 androgen receptor Mus musculus 56-59 1666408-8 1991 In contrast, [3H]PN200-110 binding showed a transient elevation in the hippocampal CA1 sector, the dentate molecular layer and the thalamus 1 h of recirculation. Tritium 14-16 carbonic anhydrase 1 Homo sapiens 83-86 1668114-2 1991 We found that YM461 inhibited [3H]PAF binding to rabbit platelet membranes with a pKi value of 8.90. Tritium 31-33 patchy fur Mus musculus 34-37 1657994-3 1991 When acini were incubated with [3H]choline in the presence of 1 nM CCK-octapeptide (CCK8) for 60 min, the incorporations of [3H]choline into both water-soluble choline metabolites and PC in acini were reduced by CCK8 to 74 and 41% of control, respectively. Tritium 32-34 cholecystokinin Rattus norvegicus 67-70 1657994-3 1991 When acini were incubated with [3H]choline in the presence of 1 nM CCK-octapeptide (CCK8) for 60 min, the incorporations of [3H]choline into both water-soluble choline metabolites and PC in acini were reduced by CCK8 to 74 and 41% of control, respectively. Tritium 125-127 cholecystokinin Rattus norvegicus 67-70 1719824-2 1991 [3H]rauwolscine bound to a single class of alpha 2-adrenoceptors with high affinity [Kd = 1.7 +/- 0.3 nM, maximum binding (Bmax) = 45.2 +/- 10.8 fmol/mg protein]. Tritium 1-3 adrenoceptor alpha 2A Homo sapiens 43-64 1744575-2 1991 In human endometrial stromal cells, bombesin and bradykinin provoked an increase in intracellular free Ca2+ and in labelled inositol phosphates when pre-incubated with [3H]myoinositol. Tritium 169-171 gastrin releasing peptide Homo sapiens 36-44 1744575-4 1991 [3H]Thymidine was added to human cultured endometrial stromal cells with bombesin, bradykinin, epidermal growth factor (EGF), prostaglandin F2 alpha, vasopressin and platelet-derived growth factor. Tritium 1-3 gastrin releasing peptide Homo sapiens 73-81 1686902-4 1991 The [3H]PDBu binding activities in the strata oriens of the CA1 and CA3 subfields and the molecular layer of the dentate gyrus in the rat hippocampus were significantly higher than in the gerbil hippocampus. Tritium 5-7 carbonic anhydrase 3 Rattus norvegicus 68-71 1811167-8 1991 After an initial 4-hour incubation of the cells with [3H]-lysine-labelled soluble elastin, most of the radioactivity in the insoluble elastin was associated with the lysine and only a negligible amount was detected in the desmosines. Tritium 54-56 elastin Rattus norvegicus 82-89 1811167-8 1991 After an initial 4-hour incubation of the cells with [3H]-lysine-labelled soluble elastin, most of the radioactivity in the insoluble elastin was associated with the lysine and only a negligible amount was detected in the desmosines. Tritium 54-56 elastin Rattus norvegicus 134-141 1928432-4 1991 By autoradiography, [3H]AVP binding sites in the rabbit bladder were shown to be located on both circularly and longitudinally oriented smooth muscle cells, as well as in the submucosa at the part adjacent to the urothelium. Tritium 21-23 vasopressin-neurophysin 2-copeptin Oryctolagus cuniculus 24-27 1782014-1 1991 The effect of 1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride (H-7), an inhibitor of protein kinase C, on the nuclear binding of [3H]dexamethasone and on the phosphorylation of glucocorticoid receptor was studied in rat liver slices to ascertain the role of protein kinase C in the expression of glucocorticoid action. Tritium 144-146 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 191-214 1661625-7 1991 When the GRP(14-27) peptide was added exogenously to breast cancer and SCLC cell lines under serum-free culture conditions, (3H)-thymidine incorporation was stimulated by GRP(14-27) in the SCLC cell lines. Tritium 125-127 gastrin releasing peptide Homo sapiens 9-12 1661625-7 1991 When the GRP(14-27) peptide was added exogenously to breast cancer and SCLC cell lines under serum-free culture conditions, (3H)-thymidine incorporation was stimulated by GRP(14-27) in the SCLC cell lines. Tritium 125-127 gastrin releasing peptide Homo sapiens 171-174 1661625-11 1991 The identification of GRP-like receptors on T47D cells is in accordance with our present observation of a growth response to GRP(14-27) as evaluated by increased (3H)-thymidine incorporation. Tritium 163-165 gastrin releasing peptide Homo sapiens 22-25 1661625-11 1991 The identification of GRP-like receptors on T47D cells is in accordance with our present observation of a growth response to GRP(14-27) as evaluated by increased (3H)-thymidine incorporation. Tritium 163-165 gastrin releasing peptide Homo sapiens 125-128 1665676-4 1991 These factors also caused significant increases in VDR levels as measured by ligand binding assays, which preceded the rise in cell proliferation rate measured by [3H]thymidine incorporation. Tritium 164-166 vitamin D (1,25-dihydroxyvitamin D3) receptor Mus musculus 51-54 1720904-7 1991 This minimal proteolysis destroyed the binding of [3H]-heparin to VN and reversed the neutralization of heparin by VN. Tritium 50-54 vitronectin Homo sapiens 66-68 1720904-7 1991 This minimal proteolysis destroyed the binding of [3H]-heparin to VN and reversed the neutralization of heparin by VN. Tritium 50-54 vitronectin Homo sapiens 115-117 1665525-6 1991 Astrocytes took up 3H-antisense GFAP RNA alone and reached an equilibrium of 7-8.8 eta g per mg protein after 2.5 hr. Tritium 19-21 glial fibrillary acidic protein Homo sapiens 32-36 1666178-5 1991 The binding of [3H] neurotensin to the purified receptor is saturable and specific, with a Kd of 0.45 nM. Tritium 16-18 neurotensin Rattus norvegicus 20-31 1796351-1 1991 The cocaine congener [3H]CFT, also designated [3H]WIN 35,428 (2 beta-carbomethoxy-3 beta-(4-fluorophenyltropane), labels cocaine receptors associated with the dopamine transporter in primate striatum. Tritium 22-24 solute carrier family 6 member 3 Homo sapiens 159-179 1796351-1 1991 The cocaine congener [3H]CFT, also designated [3H]WIN 35,428 (2 beta-carbomethoxy-3 beta-(4-fluorophenyltropane), labels cocaine receptors associated with the dopamine transporter in primate striatum. Tritium 47-49 solute carrier family 6 member 3 Homo sapiens 159-179 1650825-6 1991 In [3H]myo-inositol labeled strips of myometrial smooth muscle, the adenosine agonist R-phenylisopropyl adenosine (R-PIA) stimulated the rapid formation of inositol-1,4,5-trisphosphate (InsP3) that was antagonized by addition of the nucleoside receptor antagonist 8-sulfophenyl theophylline. Tritium 4-6 ribose-5-phosphate isomerase Cavia porcellus 86-120 1664073-4 1991 In kinetic experiments, association of [3H]ceranapril to homogenates of rat and human cortex was found to be rapid and fully reversible (K+1 = 6 x 10(5) M-1 sec-1 and 2.4 x 10(6) M-1 sec-1, K-1 = 7.6 x 10(-3) sec-1 and 4.5 x 10(-3) sec-1 respectively). Tritium 39-42 secretory blood group 1, pseudogene Homo sapiens 157-162 1664073-4 1991 In kinetic experiments, association of [3H]ceranapril to homogenates of rat and human cortex was found to be rapid and fully reversible (K+1 = 6 x 10(5) M-1 sec-1 and 2.4 x 10(6) M-1 sec-1, K-1 = 7.6 x 10(-3) sec-1 and 4.5 x 10(-3) sec-1 respectively). Tritium 39-42 secretory blood group 1, pseudogene Homo sapiens 183-188 1664073-4 1991 In kinetic experiments, association of [3H]ceranapril to homogenates of rat and human cortex was found to be rapid and fully reversible (K+1 = 6 x 10(5) M-1 sec-1 and 2.4 x 10(6) M-1 sec-1, K-1 = 7.6 x 10(-3) sec-1 and 4.5 x 10(-3) sec-1 respectively). Tritium 39-42 secretory blood group 1, pseudogene Homo sapiens 183-188 1664073-4 1991 In kinetic experiments, association of [3H]ceranapril to homogenates of rat and human cortex was found to be rapid and fully reversible (K+1 = 6 x 10(5) M-1 sec-1 and 2.4 x 10(6) M-1 sec-1, K-1 = 7.6 x 10(-3) sec-1 and 4.5 x 10(-3) sec-1 respectively). Tritium 39-42 secretory blood group 1, pseudogene Homo sapiens 183-188 1649835-4 1991 Photolabeling with an isoquinoline ligand, [3H]PK 14105, identifies a 17-kDa protein, the PBR isoquinoline binding protein (PBR/IBP), in both species. Tritium 44-46 translocator protein Bos taurus 90-93 1649835-4 1991 Photolabeling with an isoquinoline ligand, [3H]PK 14105, identifies a 17-kDa protein, the PBR isoquinoline binding protein (PBR/IBP), in both species. Tritium 44-46 translocator protein Bos taurus 124-127 1649835-4 1991 Photolabeling with an isoquinoline ligand, [3H]PK 14105, identifies a 17-kDa protein, the PBR isoquinoline binding protein (PBR/IBP), in both species. Tritium 44-46 translocator protein Bos taurus 128-131 1649835-8 1991 Transfecting the cloned bovine PBR/IBP cDNA into COS-7 cells resulted in an 11-fold increase in the density of high affinity [3H]PK 11195 binding sites which had only low affinity for Ro5-4864. Tritium 126-128 translocator protein Bos taurus 31-34 1649835-8 1991 Transfecting the cloned bovine PBR/IBP cDNA into COS-7 cells resulted in an 11-fold increase in the density of high affinity [3H]PK 11195 binding sites which had only low affinity for Ro5-4864. Tritium 126-128 translocator protein Bos taurus 35-38 1650214-15 1991 The [3H]TCDD.Ah receptor complex extracted from nuclei of Mz-Hep-1 cells incubated with [3H]TCDD in culture at 37 degrees sedimented at approximately 6 S under conditions of high ionic strength. Tritium 5-7 DNL-type zinc finger Homo sapiens 61-66 1657281-7 1991 Instead, 5-HT1D receptor binding sites should be redefined as [3H]5-HT-labeled binding sites displaced by 10(-5) M sumatriptan. Tritium 63-65 5-hydroxytryptamine receptor 1D Homo sapiens 9-15 1657279-8 1991 [3H]QNB and [3H]PN200-110 binding decreased by more than 50% in the CA1 subfield by 1 month, but [3H]CHA binding decreased transiently by 20-30% at 4 days when delayed neuronal death of hippocampal CA1 pyramidal cells took place. Tritium 1-3 carbonic anhydrase 1 Homo sapiens 68-71 1657279-8 1991 [3H]QNB and [3H]PN200-110 binding decreased by more than 50% in the CA1 subfield by 1 month, but [3H]CHA binding decreased transiently by 20-30% at 4 days when delayed neuronal death of hippocampal CA1 pyramidal cells took place. Tritium 13-15 carbonic anhydrase 1 Homo sapiens 68-71 1657279-8 1991 [3H]QNB and [3H]PN200-110 binding decreased by more than 50% in the CA1 subfield by 1 month, but [3H]CHA binding decreased transiently by 20-30% at 4 days when delayed neuronal death of hippocampal CA1 pyramidal cells took place. Tritium 13-15 carbonic anhydrase 1 Homo sapiens 68-71 1645763-4 1991 To this end, we performed contact autoradiography on tritium-sensitive film using 1.5-2.0 nmol [3H]AVP/l as ligand. Tritium 53-60 vasopressin-neurophysin 2-copeptin Ovis aries 99-102 1645763-4 1991 To this end, we performed contact autoradiography on tritium-sensitive film using 1.5-2.0 nmol [3H]AVP/l as ligand. Tritium 96-98 vasopressin-neurophysin 2-copeptin Ovis aries 99-102 1645763-8 1991 Specific [3H]AVP-binding sites formed an irregular, patchy pattern throughout the anterior lobe. Tritium 10-12 vasopressin-neurophysin 2-copeptin Ovis aries 13-16 1645763-11 1991 [3H]AVP binding was more intense in the sheep than in the rat anterior lobe, suggesting that AVP may be particularly important for ACTH secretion in the sheep. Tritium 1-3 vasopressin-neurophysin 2-copeptin Ovis aries 4-7 2015292-5 1991 Rabbit muscle glyceraldehyde-3-phosphate dehydrogenase labeled with [3H]koningic acid was digested with trypsin. Tritium 69-71 glyceraldehyde-3-phosphate dehydrogenase Oryctolagus cuniculus 14-54 2004614-4 1991 TGF alpha and TGF beta each inhibit GH4 cell proliferation, as measured by cell number and [3H]thymidine incorporation, and given together arrest GH4 cell proliferation. Tritium 92-94 transforming growth factor alpha Rattus norvegicus 0-9 2005092-8 1991 Inhibition of both cholesterol transfer and esterification with 3-(decyldimethylsilyl)-N-[2-(4-methylphenyl)-1-phenylethyl]propanamide from Sandoz abolished the L-FABP related enhancement of both [3H]cholesterol uptake and esterification. Tritium 197-199 fatty acid binding protein 1, liver Mus musculus 161-167 2005092-15 1991 Concomitantly, accessibility of plasma membrane [3H]cholesterol to cholesterol oxidase was decreased 18 +/- 3% in cells expressing L-FABP. Tritium 49-51 fatty acid binding protein 1, liver Mus musculus 131-137 1988299-1 1991 Xanthine oxidase stimulates [3H]thymidine incorporation by 3T3 cells even in the absence of any added xanthine, but in the presence of, and synergistically with, insulin or various other growth-stimulating factors. Tritium 29-31 xanthine dehydrogenase Mus musculus 0-16 1992016-6 1991 Very dense 3H-CP55,940 binding is localized to the basal ganglia (lateral caudate-putamen, globus pallidus, entopeduncular nucleus, substantia nigra pars reticulata), cerebellar molecular layer, innermost layers of the olfactory bulb, and portions of the hippocampal formation (CA3 and dentate gyrus molecular layer). Tritium 11-13 carbonic anhydrase 3 Rattus norvegicus 278-281 1988771-7 1991 Approximately 1% of dietary desmosine (ingested as [3H]elastin and [3H] desmosine) was excreted in the urine within 24 hours, contributing approximately 15% of UD while on a normal diet. Tritium 52-54 elastin Homo sapiens 55-62 1846353-1 1991 In Swiss 3T3 fibroblasts a peptide mitogen bombesin, which acts through the phospholipase C-protein kinase C signaling pathway, stimulates DNA synthesis in a manner strictly dependent on the medium calcium concentration: [3H]thymidine incorporation into DNA in the presence of a saturating concentration of bombesin (10(-8) M) is 4-fold greater at 3.0 mM extracellular calcium as compared with a value obtained at 0.03 mM calcium. Tritium 222-224 gastrin releasing peptide Homo sapiens 43-51 1959325-6 1991 The purified frog ODC showed three bands on SDS-polyacrylamide gel electrophoretic analysis, as confirmed by [3H]alpha-difluoromethylornithine binding. Tritium 110-112 ornithine decarboxylase, structural 1 Mus musculus 18-21 1657685-2 1991 The effect of neuropeptide (NPY) on [3H]noradrenaline ([3H]NA) release- and on contractions evoked by field electrical stimulation (FES) was studied in vitro in vas deferens from mature and immature guinea pigs. Tritium 37-39 pro-neuropeptide Y Cavia porcellus 28-31 1657685-13 1991 NPY, 1 microM, inhibited the evoked tritium overflow. Tritium 36-43 pro-neuropeptide Y Cavia porcellus 0-3 1681616-8 1991 Collagenolytic activity in serum-free RCM-1 conditioned medium was localized in the G-body and MV fractions (80% and 20% of the total activity, respectively, when tested against 3H-labeled type I collagen). Tritium 178-180 troponin I3, cardiac type Homo sapiens 38-43 1979919-9 1990 With polyclonal antibodies raised against aminopeptidase N from pig renal microsomes the aminopeptidase N from solubilized brush border membranes from pig small intestine could be completely precipitated; the binding protein for beta-lactam antibiotics and oligopeptides of apparent Mr 127,000 identified by direct photoaffinity labeling with [3H]benzylpenicillin showed no crossreactivity with the aminopeptidase N anti serum and was not precipitated by the anti serum. Tritium 344-346 alanyl aminopeptidase, membrane Sus scrofa 42-58 2246232-2 1990 The present study documents that [3H]PAF (3.5 mu Ci) injected into the renal artery of isolated control rat kidney preparations perfused at constant pressure with a cell-free medium containing 1% bovine serum albumin (BSA) was excreted in negligible amounts (0.034%) in the urine, whereas 6% was retained by the kidney. Tritium 34-36 PCNA clamp associated factor Rattus norvegicus 37-40 2246232-4 1990 [3H]PAF urine excretion in proteinuric kidneys from adriamycin-treated rats was still negligible (0.015%). Tritium 1-3 PCNA clamp associated factor Rattus norvegicus 4-7 2213023-2 1990 The binding of [3H]CGS 21680, as determined by standard radioligand binding technique in the presence of exogenously added adenosine deaminase, reached equilibrium after 40 min at 25 degrees C. In saturation studies, a single class of high-affinity binding sites with values for KD of 22 +/- 0.5 nM and Bmax of 444 +/- 63 fmol/mg of protein were observed. Tritium 16-18 adenosine deaminase Rattus norvegicus 123-142 2272361-5 1990 Competition with eltoprazine for [3H]ligand binding to the various 5-HT1 receptor subtypes revealed preferential binding to 5-HT1A (IC50 values ranging from 42 to 50 nM) and 5-HT1B (IC50 values ranging from 25 to 38 nM) recognition sites. Tritium 34-36 5-hydroxytryptamine receptor 1B Rattus norvegicus 174-180 1975955-2 1990 Oocytes injected with GAT-1 synthetic messenger RNA accumulated [3H]GABA to levels above control values. Tritium 65-67 solute carrier family 6 member 12 Rattus norvegicus 22-27 2390263-5 1990 In mouse fetal lung organ cultures, the addition of bombesin led to accelerated uptake of [3H]thymidine into DNA, [3H]leucine into protein, and [3H]choline into SPC, indicating that increased growth and maturation may be direct effects. Tritium 91-93 gastrin releasing peptide Homo sapiens 52-60 2390263-5 1990 In mouse fetal lung organ cultures, the addition of bombesin led to accelerated uptake of [3H]thymidine into DNA, [3H]leucine into protein, and [3H]choline into SPC, indicating that increased growth and maturation may be direct effects. Tritium 115-117 gastrin releasing peptide Homo sapiens 52-60 2390263-5 1990 In mouse fetal lung organ cultures, the addition of bombesin led to accelerated uptake of [3H]thymidine into DNA, [3H]leucine into protein, and [3H]choline into SPC, indicating that increased growth and maturation may be direct effects. Tritium 115-117 gastrin releasing peptide Homo sapiens 52-60 2169313-4 1990 The [3H]thymidine incorporation stimulated by other growth factors (aFGF, bFGF and bombesin) was not inhibited by the antibody. Tritium 5-7 gastrin releasing peptide Homo sapiens 83-91 2167151-1 1990 In [3H]inositol-labeled membranes prepared from Swiss mouse 3T3 and human small cell lung carcinoma cells, [Tyr4]-bombesin stimulated production of water-soluble inositol phosphates. Tritium 4-6 gastrin releasing peptide Homo sapiens 114-122 2215358-2 1990 The susceptibility of stable [3H] elastin-fatty acid complexes to the action of porcine pancreatic elastase (PPE) and to human neutrophil lysates over time was assessed. Tritium 30-32 elastin Homo sapiens 34-41 2194573-11 1990 The sensitivity of [3H]myristate and [3H]palmitate bonds to 2-mercaptoethanol further suggests that either fatty acid is linked via thioester rather than hydroxyester bonds to each domain on CD9. Tritium 20-22 CD9 molecule Homo sapiens 191-194 2194573-12 1990 Limited proteolysis analysis with Staphylococcus aureus V8 proteinase of CD9, labeled in the absence or presence of cycloheximide, showed that [3H]myristic acid and [3H]palmitic acid labeled identical peptides, and to the same extent, suggesting that myristate is an alternative substrate for the transacylase(s) involved. Tritium 144-147 CD9 molecule Homo sapiens 73-76 2194573-12 1990 Limited proteolysis analysis with Staphylococcus aureus V8 proteinase of CD9, labeled in the absence or presence of cycloheximide, showed that [3H]myristic acid and [3H]palmitic acid labeled identical peptides, and to the same extent, suggesting that myristate is an alternative substrate for the transacylase(s) involved. Tritium 144-146 CD9 molecule Homo sapiens 73-76 2161838-1 1990 Time-dependent formation of a guanine nucleotide-sensitive receptor state from which [3H]bradykinin dissociates slowly. Tritium 86-88 kininogen 1 Bos taurus 89-99 2161838-2 1990 We have examined the binding of [3H]bradykinin to bovine myometrial membranes and assessed its sensitivity to guanine nucleotides. Tritium 33-35 kininogen 1 Bos taurus 36-46 2161838-10 1990 These results suggest that [3H]bradykinin interacts with at least two B2 kinin receptor-like binding sites in bovine myometrial membranes. Tritium 28-30 kininogen 1 Bos taurus 31-41 2194077-4 1990 Digestion of tumor nuclei with deoxyribonuclease I (DNase I) prior to incubation with [3H]ER in vitro resulted in a progressive loss of [3H]ER binding capacity, which was not accompanied by alterations in the affinity of [3H]ER for the nuclei (Kd = 1-3 nM). Tritium 87-89 deoxyribonuclease 1 Rattus norvegicus 31-50 2194077-4 1990 Digestion of tumor nuclei with deoxyribonuclease I (DNase I) prior to incubation with [3H]ER in vitro resulted in a progressive loss of [3H]ER binding capacity, which was not accompanied by alterations in the affinity of [3H]ER for the nuclei (Kd = 1-3 nM). Tritium 87-89 deoxyribonuclease 1 Rattus norvegicus 52-59 2194077-4 1990 Digestion of tumor nuclei with deoxyribonuclease I (DNase I) prior to incubation with [3H]ER in vitro resulted in a progressive loss of [3H]ER binding capacity, which was not accompanied by alterations in the affinity of [3H]ER for the nuclei (Kd = 1-3 nM). Tritium 137-139 deoxyribonuclease 1 Rattus norvegicus 31-50 2194077-4 1990 Digestion of tumor nuclei with deoxyribonuclease I (DNase I) prior to incubation with [3H]ER in vitro resulted in a progressive loss of [3H]ER binding capacity, which was not accompanied by alterations in the affinity of [3H]ER for the nuclei (Kd = 1-3 nM). Tritium 137-139 deoxyribonuclease 1 Rattus norvegicus 52-59 2194077-4 1990 Digestion of tumor nuclei with deoxyribonuclease I (DNase I) prior to incubation with [3H]ER in vitro resulted in a progressive loss of [3H]ER binding capacity, which was not accompanied by alterations in the affinity of [3H]ER for the nuclei (Kd = 1-3 nM). Tritium 137-139 deoxyribonuclease 1 Rattus norvegicus 31-50 2194077-4 1990 Digestion of tumor nuclei with deoxyribonuclease I (DNase I) prior to incubation with [3H]ER in vitro resulted in a progressive loss of [3H]ER binding capacity, which was not accompanied by alterations in the affinity of [3H]ER for the nuclei (Kd = 1-3 nM). Tritium 137-139 deoxyribonuclease 1 Rattus norvegicus 52-59 2194077-5 1990 A significantly lower concentration (P less than 0.005) of DNase I eliminated 50% of the [3H]ER binding sites in nuclei of tumors from intact hosts (8 unit.min/ml) compared to tumors from ovex hosts (22 unit.min/ml). Tritium 90-92 deoxyribonuclease 1 Rattus norvegicus 59-66 2194077-7 1990 The amount of DNase I required to eliminate 50% of [3H]ER binding to nuclei from lactating mammary gland, liver, and kidney ranged from 14 to 56 unit.min/ml. Tritium 52-54 deoxyribonuclease 1 Rattus norvegicus 14-21 2160463-7 1990 The extent and rate of [3H]arachidonic acid release from prelabeled P388D1 cells are also increased in primed cells relative to unprimed cells in response to PAF-stimulation. Tritium 24-26 patchy fur Mus musculus 158-161 2160811-2 1990 In order to delineate the mechanism of the transport of an intact tripeptide across the brush-border membrane, we studied the characteristics of the uptake of [3H]Phe-Pro-Ala in membrane vesicles in which the activity of dipeptidylpeptidase IV was completely inhibited by treatment with di-isopropyl fluorophosphate. Tritium 160-163 dipeptidyl peptidase 4 Oryctolagus cuniculus 221-243 2195988-2 1990 The activities of DDP and JM8 were also compared in an antimetabolic assay for their ability to inhibit (3H)-thymidine incorporation in multicellular tumor spheroids. Tritium 105-107 calcium voltage-gated channel subunit alpha1 F Homo sapiens 26-29 1694464-13 1990 These results indicate that both central and peripheral NK3 receptors share a similar pharmacological specificity and that they may be labelled selectively with the NK3 receptor agonist [3H]-senktide. Tritium 187-189 neuromedin-K receptor Cavia porcellus 56-68