PMID-sentid	Pub_year	Sent_text	compound_name	comp_offset	prot_official_name	organism	prot_offset
30936009-2	2019	This work aimed to get a novel polysaccharide, termed MRP-1, which was isolated from Moringa oleifera roots with hot water extraction method followed by ethanol precipitation and purified with DEAE-Sepharose Fast Flow column.	deae-sepharose	193-207	prolactin family 2, subfamily c, member 2	Mus musculus	54-59
30647687-1	2017	The chromatography of deoxyribonuclease (DNase) from small intestine of camel Camelus dromedarius by DEAE-Sepharose separated three isoforms DNase 1, DNase 2 and DNase 3.	deae-sepharose	101-115	deoxyribonuclease-1	Camelus dromedarius	141-148
30647687-1	2017	The chromatography of deoxyribonuclease (DNase) from small intestine of camel Camelus dromedarius by DEAE-Sepharose separated three isoforms DNase 1, DNase 2 and DNase 3.	deae-sepharose	101-115	deoxyribonuclease-2-alpha	Camelus dromedarius	150-157
19620694-9	2010	Through only two steps using reduced formyl-Cellulofine and DEAE-Sepharose, HMW adiponectin can be effectively purified from human plasma.	deae-sepharose	60-74	adiponectin, C1Q and collagen domain containing	Homo sapiens	80-91
26256355-1	2015	In this study, a water-soluble polysaccharide (CSP) was successfully purified from Chaenomeles speciosa by DEAE-Sepharose and Sephadex G-100 column chromatography.	deae-sepharose	107-121	DnaJ heat shock protein family (Hsp40) member C5	Mus musculus	47-50
25574816-4	2015	Their dephosphorylation by recombinant human PHPT1 was investigated by using a DEAE-Sepharose spin column technique earlier developed by us for studies on basic phosphoproteins and phosphopeptides.	deae-sepharose	79-93	phosphohistidine phosphatase 1	Homo sapiens	45-50
18056116-3	2008	In RAO-3 cells, Sephacryl-S 300 gel filtration and DEAE-Sepharose ion exchange chromatography demonstrated that AKR1B10 exists in two distinct forms, monomers (approximately 40 kDa) bound to DEAE-Sepharose column and protein complexes (approximately 300 kDa) remaining in flow-through.	deae-sepharose	51-65	aldo-keto reductase family 1 member B10	Homo sapiens	112-119
19223030-1	2009	A novel acidic heteropolysaccharide (TAPA1) was purified from hot water extracts of Tremella aurantialba fruiting bodies by DEAE-Sepharose Fast Flow and Sephacryl S-500 High-Resolution Chromatography.	deae-sepharose	124-138	CD81 antigen	Mus musculus	37-42
20027148-5	2009	Apo-cyt b5 was separated from myoglobin by chromatography on DEAE-Sepharose.	deae-sepharose	61-75	cytochrome b5 type A	Equus caballus	4-10
20027148-8	2009	The apo-cyt b5 protein was separated from myoglobin present in the reaction mixture by chromatography on a column of DEAE-Sepharose.	deae-sepharose	117-131	cytochrome b5 type A	Equus caballus	8-14
18757189-1	2009	A novel lectin (PCL) with specificity towards sialic acid was purified from Phaseolus coccineus L. (P. multiflorus willd) seeds using ion exchange chromatography on CM and DEAE-Sepharose, and gel filtration on Sephacryl S-200 column.	deae-sepharose	172-186	polycystic kidney disease 2-like 1	Mus musculus	16-19
18056116-3	2008	In RAO-3 cells, Sephacryl-S 300 gel filtration and DEAE-Sepharose ion exchange chromatography demonstrated that AKR1B10 exists in two distinct forms, monomers (approximately 40 kDa) bound to DEAE-Sepharose column and protein complexes (approximately 300 kDa) remaining in flow-through.	deae-sepharose	191-205	aldo-keto reductase family 1 member B10	Homo sapiens	112-119
16122899-9	2006	The rh-prolidase purified from the supernatant by ion exchange gradient chromatography (DEAE-Sepharose Fast Flow) and gel filtration chromatography (Sephacryl S-200 High Resolution) showed a single band by SDS-PAGE analysis.	deae-sepharose	88-102	peptidase D	Homo sapiens	7-16
17827718-2	2007	In this study, PAF-AH in early colostrum was purified by ammonium sulfate precipitation, and sequential use of butyl-Toyopearl 650M, DEAE-Sepharose, heparin-Sepharose, hydroxyapatite, chelating-Sepharose and Mono Q HPLC column chromatography.	deae-sepharose	133-147	phospholipase A2 group VII	Homo sapiens	15-21
16237753-2	2005	METHODS: Hsp70-peptide complexes were purified from human HCC cells with column chromatography using ADP-agarose and DEAE-Sepharose.	deae-sepharose	117-131	heat shock protein family A (Hsp70) member 4	Homo sapiens	9-14
16309179-1	2005	Membrane vesicles from the multidrug-resistant KB-V1 and KB-C1 cell lines overexpressing P-glycoprotein (Pgp), responsible for pleiotropic chemotherapeutic agents resistance, were solubilized with octyl-glucoside (OG-EX) and further fractionated on DEAE-sepharose column with increased concentrations of NaCl.	deae-sepharose	249-263	ATP binding cassette subfamily B member 1	Homo sapiens	105-108
15902422-1	2005	The recombinant human liver prolidase (rh-prolidase, EC 3.4.13.9) from the lysate supernatant of engineering yeast Saccharomyces cerevisiae was purified in two steps employing anion-exchange gradient chromatography (DEAE-Sepharose fast flow) and gel filtration chromatography (Sephacryl S-200 high resolution).	deae-sepharose	216-230	peptidase D	Homo sapiens	28-37
15902422-1	2005	The recombinant human liver prolidase (rh-prolidase, EC 3.4.13.9) from the lysate supernatant of engineering yeast Saccharomyces cerevisiae was purified in two steps employing anion-exchange gradient chromatography (DEAE-Sepharose fast flow) and gel filtration chromatography (Sephacryl S-200 high resolution).	deae-sepharose	216-230	peptidase D	Homo sapiens	42-51
15115769-3	2004	PGIS was expressed in the microsomes of the infected Sf21 cells after culture in 5 microg/ml hematin-supplemented medium for 72 h. The holoenzyme was isolated from the solubilized microsomal fraction by calcium phosphate gel absorption and purified to homogeneity by DEAE-Sepharose and hydroxyapatite column chromatography.	deae-sepharose	267-281	prostaglandin I2 synthase	Homo sapiens	0-4
14520003-1	2003	The stepwise chromatographic behaviour on DEAE-Sepharose of rat Fe65, a neuronal protein, was tested, using as eluants KCl, CaCl2, and MgCl2.	deae-sepharose	42-56	amyloid beta precursor protein binding family B member 1	Rattus norvegicus	64-68
12757412-1	2003	An antitumour lectin (named AAL) consisting of two identical subunits of 15.8 kDa was isolated from the fruiting bodies of the edible mushroom Agrocybe aegerita using a procedure which involved precipitating the extract by addition of (NH(4))(2)SO(4), ion exchange chromatography on DEAE-Sepharose Fast Flow, gel filtration chromatography on Sephacryl S-200 HR and finally purification on a GF-250 HPLC column.	deae-sepharose	283-297	active avoidance learning	Mus musculus	28-31
12385237-7	2002	BL21 (DE3) respectively, we got 3 kinds of engineered E. coli which express TPO + Fc chimeric proteins(28 kD TPO + Fc1, 12 kD TPO + Fc2 and 12 kD TPO + Fc3) at high level respectively, the expressed proteins were purified with DEAE-Sepharose FF and S-Sepharose FF column.	deae-sepharose	227-241	thrombopoietin	Mus musculus	76-79
11687588-3	2002	NDH II was partially purified from HeLa nuclear extracts by ion-exchange chromatography on Bio-Rex 70 and DEAE-Sepharose.	deae-sepharose	106-120	DExH-box helicase 9	Homo sapiens	0-6
12390762-2	2002	METHODS: A novel purification method established in our laboratory was adopted for the purification of the inclusion body, and after renaturation, recombinant human IL2-PE66(4Glu) fusion protein was purified by DEAE-Sepharose FF ion-exchange chromatography.	deae-sepharose	211-225	interleukin 2	Homo sapiens	165-168
11404768-8	2001	The rSPE B/SCP was purified from the supernatant by sequential chromatography on DEAE-Sepharose, matrix gel Red A and Sephadex G-50 columns.	deae-sepharose	81-95	cysteine-rich secretory protein 3	Rattus norvegicus	4-14
11694005-1	2001	A selenium-containing peroxidase from the germinating barley grown on a selenium-containing artificial medium was isolated and purified by means of cold acetone precipitation, Sephadex-G150 filtration, followed by DEAE-Sepharose chromatography and sodium dodecyl sulfate--polyacrylamid gel electrophoresis.	deae-sepharose	214-228	prx7	Hordeum vulgare	22-32
10814785-6	2000	The brain haemopoietic biglycan was easily separated by DEAE-Sepharose chromatography from the macrophage colony stimulating factor (M-CSF) which was concomitantly produced from the brain cells.	deae-sepharose	56-70	biglycan	Rattus norvegicus	23-31
11237690-1	2001	Glucose-6-phosphate dehydrogenase was purified from human placenta using DEAE-Sepharose fast flow, 2",5"-ADP Sepharose 4B column chromatography, and chromatofocusing on PBE 94 with PB 74.	deae-sepharose	73-87	glucose-6-phosphate dehydrogenase	Homo sapiens	0-33
11049745-4	2000	The calreticulin was expressed in Pichia pastoris and purified to homogeneity by DEAE-Sepharose and Resource Q FPLC chromatography.	deae-sepharose	81-95	calreticulin	Oryctolagus cuniculus	4-16
10934208-3	2000	PP2C alpha and PP2C beta 2 co-purified with Mg(2+)-dependent Cdk2/Cdk6 phosphatase activity in DEAE-Sepharose, Superdex-200, and Mono Q chromatographies.	deae-sepharose	95-109	protein phosphatase, Mg2+/Mn2+ dependent 1A	Homo sapiens	0-10
10934208-3	2000	PP2C alpha and PP2C beta 2 co-purified with Mg(2+)-dependent Cdk2/Cdk6 phosphatase activity in DEAE-Sepharose, Superdex-200, and Mono Q chromatographies.	deae-sepharose	95-109	protein phosphatase, Mg2+/Mn2+ dependent 1B	Homo sapiens	15-24
10934208-3	2000	PP2C alpha and PP2C beta 2 co-purified with Mg(2+)-dependent Cdk2/Cdk6 phosphatase activity in DEAE-Sepharose, Superdex-200, and Mono Q chromatographies.	deae-sepharose	95-109	cyclin dependent kinase 2	Homo sapiens	61-65
10934208-3	2000	PP2C alpha and PP2C beta 2 co-purified with Mg(2+)-dependent Cdk2/Cdk6 phosphatase activity in DEAE-Sepharose, Superdex-200, and Mono Q chromatographies.	deae-sepharose	95-109	cyclin dependent kinase 6	Homo sapiens	66-70
10329783-1	1999	Acuthrombin-B, a thrombin-like enzyme from Agkistrodon acutus venom, has been isolated and purified to homogeneity by ion-exchange chromatography on DEAE-Sepharose, gel filtration on Sephacryl S-100 and fast performance liquid chromatography on DEAE-8HR.	deae-sepharose	149-163	prothrombin	Oryctolagus cuniculus	3-11
10833404-9	2000	The cytochrome b(5) was purified from detergent-solubilized bacterial membranes using anion-exchange chromatography on DEAE-Sepharose followed by size-exclusion chromatography on Superdex-75.	deae-sepharose	119-133	cytochrome b5	Oryctolagus cuniculus	4-19
12075418-1	2000	An acidic phospholipase A(2) from Agkistrondon acutus venom has been purified to homogeneity via four steps using CM-Sepharose, two times DEAE-Sepharose, and Mono Q FPLC.	deae-sepharose	138-152	phospholipase A2 group IB	Homo sapiens	10-28
9733854-6	1998	After partial purification using three different columns (DEAE-Sepharose, Econo S, and heparin-agarose), LRP was primarily associated with cdk2-cyclin E complexes, an enzyme which is necessary for G1-to-S-phase cell cycle progression.	deae-sepharose	58-72	ribosomal protein SA	Homo sapiens	105-108
10103041-3	1999	The protein, designated Pen c 1, was purified by sequential DEAE-Sepharose and carboxymethyl (CM)-Sepharose chromatographies.	deae-sepharose	60-74	proprotein convertase subtilisin/kexin type 1 inhibitor	Homo sapiens	24-27
10103041-3	1999	The protein, designated Pen c 1, was purified by sequential DEAE-Sepharose and carboxymethyl (CM)-Sepharose chromatographies.	deae-sepharose	60-74	heterogeneous nuclear ribonucleoprotein C	Homo sapiens	28-31
7876143-5	1995	The IFN gamma R-IgG3 fusion protein was secreted into the culture medium as a 175-kDa glycoprotein and was purified over Protein G-Sepharose, DEAE-Sepharose, and size exclusion chromatography.	deae-sepharose	142-156	interferon gamma	Cricetulus griseus	4-13
22358898-3	1997	The (His)(6)-LECT2 was purified from the soluble fraction in E. coli as a single band in sodium dodesyl sulfate/polyacrylamide gel electrophoresis using three steps of column chromatography with Ni(2+)-charged nitrilo-triacetic acid (Ni-NTA) agarose, DEAE-Sepharose, and CM-Sepharose.	deae-sepharose	251-265	leukocyte cell derived chemotaxin 2	Homo sapiens	13-18
9224773-5	1997	Catalytically active human FMO3 was purified to apparent homogeneity by cholate solubilization and sequential column chromatography on Octyl-Sepharose, DEAE-Sepharose, and hydroxyapatite.	deae-sepharose	152-166	flavin containing dimethylaniline monoxygenase 3	Homo sapiens	27-31
9003440-1	1997	Hepsin, a putative cell-surface serine proteinase, has been isolated from the microsomal membranes of rat liver and purified to homogeneity by hydroxyapatite, DEAE-Sepharose, and benzamidine-Sepharose chromatography.	deae-sepharose	159-173	hepsin	Rattus norvegicus	0-6
8641674-3	1996	On DEAE-Sepharose column chromatography of the extracts preparted from the cells incubated without retinoic acid, the PP2A activities determined with MBP were eluted at 0.13 M or 0.23 m NaCl, respectively.	deae-sepharose	3-17	protein phosphatase 2 phosphatase activator	Homo sapiens	118-122
8557373-2	1996	This factor, designated suppressive factor 1 (SF1), was isolated from the supernatant of sonicated whole bacteria and purified by Q-Sepharose Fast Flow column chromatography, DEAE-Sepharose Fast Flow column chromatography, hydroxyapatite high-pressure liquid chromatography (HPLC), and Protein Pack 300 & 125 gel filtration HPLC.	deae-sepharose	175-189	splicing factor 1	Mus musculus	46-49
7548180-9	1995	The PLD activity was enriched 15-fold by solubilization and purification on a DEAE-Sepharose column.	deae-sepharose	78-92	glycosylphosphatidylinositol specific phospholipase D1	Homo sapiens	4-7
7794533-2	1995	Refolded active rhAChE was purified by DEAE-Sepharose and affinity chromatography to apparent homogeneity with a specific activity (4572 units/mg) similar to that of erythrocyte AChE.	deae-sepharose	39-53	acetylcholinesterase (Cartwright blood group)	Homo sapiens	18-22
7787299-2	1995	PAS-4 was selectively extracted with Triton X-114 nonionic detergent and then fractionated on DEAE-Sepharose at pH 7.5.	deae-sepharose	94-108	PAS-4	Bos taurus	0-5
7787299-3	1995	The PAS-4 fraction that was not bound on DEAE-Sepharose gave a single band by SDS-PAGE.	deae-sepharose	41-55	PAS-4	Bos taurus	4-9
9631524-5	1998	The GST-Bax fusion protein was bound to glutathione-Sepharose, and Bax was released by thrombin cleavage and further purified by sequential chromatography on heparin-Sepharose and DEAE-Sepharose.	deae-sepharose	180-194	BCL2 associated X, apoptosis regulator	Homo sapiens	8-11
9631524-5	1998	The GST-Bax fusion protein was bound to glutathione-Sepharose, and Bax was released by thrombin cleavage and further purified by sequential chromatography on heparin-Sepharose and DEAE-Sepharose.	deae-sepharose	180-194	BCL2 associated X, apoptosis regulator	Homo sapiens	67-70
9325148-5	1997	Purification by ion-exchange chromatography on DEAE-Sepharose yielded more than 2 mg of H-PST.	deae-sepharose	47-61	sulfotransferase family 1A member 1	Homo sapiens	90-93
9056251-3	1997	This PP2A of the 18-h treated cells was eluted from a DEAE-Sepharose column with 0.13 M NaCl, while PP2A from control cells was eluted with 0.23 M NaCl.	deae-sepharose	54-68	protein phosphatase 2 phosphatase activator	Homo sapiens	5-9
8811836-3	1996	A series of chromatographic steps (DEAE-Sepharose, Sephacryl S-200 and Activated Thiol Sepharose 4B) generated a soluble PAP activity purified to near homogeneity with a total active yield of 6.6% The enzyme displayed a native molecular mass of approximately 23,700 Da, which compares well with that value obtained under denaturing conditions via SDS-PAGE (24,000 Da), suggesting that the enzyme exists as a monomer.	deae-sepharose	35-49	PDGFA associated protein 1	Homo sapiens	121-124
9187498-0	1996	Purification of phosphoglycerate kinase and glyceraldehyde phosphate dehydrogenase by DEAE-sepharose fast flow chromatography.	deae-sepharose	86-100	glyceraldehyde-3-phosphate dehydrogenase	Homo sapiens	44-82
9187498-1	1996	The purification of phosphoglycerate kinase (PGK) and glyceraldehyde phosphate dehydrogenase (GAPDH) from soy bean was studied by a DEAE-sepharose fast flow chromatography.	deae-sepharose	132-146	glyceraldehyde-3-phosphate dehydrogenase	Homo sapiens	54-92
9187498-1	1996	The purification of phosphoglycerate kinase (PGK) and glyceraldehyde phosphate dehydrogenase (GAPDH) from soy bean was studied by a DEAE-sepharose fast flow chromatography.	deae-sepharose	132-146	glyceraldehyde-3-phosphate dehydrogenase	Homo sapiens	94-99
7876143-5	1995	The IFN gamma R-IgG3 fusion protein was secreted into the culture medium as a 175-kDa glycoprotein and was purified over Protein G-Sepharose, DEAE-Sepharose, and size exclusion chromatography.	deae-sepharose	142-156	immunoglobulin heavy constant gamma 3 (G3m marker)	Homo sapiens	16-20
7999002-2	1994	Native protein kinase C-eta was partially purified from the cytosol of murine brain by chromatography on DEAE-Sepharose, hydroxyapatite and protamine-agarose.	deae-sepharose	105-119	endothelin receptor type A	Mus musculus	24-27
8207004-8	1994	ZZ-tagged Pkc1p was partially purified by chromatography on DEAE-Sepharose, followed by IgG-Sepharose.	deae-sepharose	60-74	protein kinase C	Saccharomyces cerevisiae S288C	10-15
7685022-3	1993	A terminal transferase activity that was found to cofractionate with RdRP from DEAE-Sepharose and DNA-cellulose columns was removed by chromatography on a Mono Q column.	deae-sepharose	79-93	RNA-directed RNA polymerase	Solanum lycopersicum	69-73
8195168-2	1994	p20 co-eluted with alpha B crystallin and HSP27/28 during column chromatography on DEAE-Sepharose and on Bio-Gel A-5m.	deae-sepharose	83-97	tubulin polymerization promoting protein family member 3	Homo sapiens	0-3
8250928-2	1993	After chromatography on hydroxyapatite, DEAE-Sepharose chromatography resolved the CYP2E1-degrading activity into two peaks, and the two proteinases were finally purified on benzamidine-Sepharose.	deae-sepharose	40-54	cytochrome P450, family 2, subfamily e, polypeptide 1	Rattus norvegicus	83-89
8336074-4	1993	The p64 was purified from cytosol of human PMNs (1.8 x 10(9) cells) by DEAE-Sepharose CL6B column chromatography, hydroxyapatite HPLC, and reverse-phase HPLC.	deae-sepharose	71-85	interleukin 2 receptor subunit gamma	Homo sapiens	4-7
8382169-1	1993	Anion-exchange chromatography of solubilized pig liver cell membranes on DEAE-Sepharose gave a fraction with high affinity binding proteins for VIP and glucagon distinct from each other.	deae-sepharose	73-87	vasoactive intestinal peptide	Sus scrofa	144-147
7763539-1	1993	A novel immunomodulating fraction, SP-MAF1, was isolated from soybeans as a polysaccharide-protein complex by hot water extraction followed by acid treatment, ethanol treatment, and chromatography on a DEAE-Sepharose CL-6B column, and was characterized in some biological activities.	deae-sepharose	202-216	MFP1 attachment factor 1	Glycine max	38-42
1326533-3	1992	The membrane-associated cytochrome b558 was solubilized with a detergent, n-heptyl beta-thioglucoside, and purified by DEAE-Sepharose, heparin-Sepharose, and Mono Q column chromatography.	deae-sepharose	119-133	cytochrome b	Oryctolagus cuniculus	24-36
1334488-2	1992	A novel human tissue kallikrein inhibitor designated as kallistatin has been purified from plasma to apparent homogeneity by polyethylene glycol fractionation and successive chromatography on heparin-Agarose, DEAE-Sepharose, hydroxylapatite, and phenyl-Superose columns.	deae-sepharose	209-223	serpin family A member 4	Homo sapiens	56-67
1280424-4	1992	MDGI 2 was purified by chromatography over DEAE-Sepharose, Bio-Gel P-30 in 1% acetic acid, Sephacryl S-200 in 6 M urea and Mono Q.	deae-sepharose	43-57	fatty acid binding protein 3	Bos taurus	0-4
1331100-6	1992	A salt extract from canine pancreatic ER membranes and cytosol possessed SSR alpha kinase activity which showed identical chromatographic behavior through phosphocellulose, DEAE-Sepharose, and hydroxylapatite purification protocols.	deae-sepharose	173-187	signal sequence receptor subunit 1	Canis lupus familiaris	73-82
1326560-2	1992	The cytochrome b558 was solubilized from membranes with the detergent n-heptyl-beta-thioglucoside and purified by DEAE-Sepharose and heparin-Sepharose chromatographies.	deae-sepharose	114-128	cytochrome b	Sus scrofa	4-16
1560006-1	1992	Immunoreactive alpha B crystallin and a 28-kDa protein in an extract of human pectoral muscle were precipitated by (NH4)2SO4 at 40% saturation, and coeluted during column chromatography on DEAE-Sepharose and on Bio-Gel A-5m.	deae-sepharose	189-203	crystallin alpha B	Bos taurus	15-33
1413649-1	1992	Highly purified preparation of prolactin with molecular mass 23 kDa, which is the main form of the hormone in human amnionic fluid, was isolated from the fluid using gel filtration on Sephadex G-100, chromatography on DEAE-Sepharose, concanavalin A Sepharose, CM-cellulose.	deae-sepharose	218-232	prolactin	Homo sapiens	31-40
1591243-4	1992	The c-fes cDNA was expressed at high levels in a baculovirus system, and the catalytically active recombinant c-fes gene product p93c-fes was partially purified by DEAE-Sepharose and tyrosine-agarose chromatography.	deae-sepharose	164-178	FES proto-oncogene, tyrosine kinase	Homo sapiens	129-137
1730869-10	1992	The majority of nonspecific GIF in the culture supernatant was recovered from DEAE-Sepharose by elution of the column with 10 mM Tris-HCl buffer, pH 8.0, containing 50 mM NaCl.	deae-sepharose	78-92	cobalamin binding intrinsic factor	Homo sapiens	28-31
1838311-2	1991	Annexin V has been purified from Triton X-100 extracts of porcine gastric mucosal membranes by a combination of chromatography on concanavalin A-Sepharose and DEAE-Sepharose, and preparative gel electrophoresis.	deae-sepharose	159-173	annexin A5	Homo sapiens	0-9
1764068-1	1991	S-Adenosylmethionine decarboxylase (EC 4.1.1.19) was purified to homogeneity from the cytosol of soybean (Glycine max) axes by ammonium sulfate fractionation, DEAE-Sepharose and methylglyoxalbis(guanylhydrazone)-Sepharose 6B chromatographies.	deae-sepharose	159-173	S-adenosylmethionine decarboxylase	Glycine max	0-34
1907914-1	1991	The elongation factor 1 alpha (aEF-1 alpha) was purified to homogeneity from the thermoacidophilic archaebacterium Sulfolobus solfataricus by chromatographic procedures utilising DEAE-Sepharose, hydroxyapatite and FPLC on Mono S. The purified protein binds [3H]GDP at a 1:1 molar ratio and it is essential for poly(Phe) synthesis in vitro; it also binds GTP but not ATP.	deae-sepharose	179-193	Hsp20/alpha crystallin family protein	Saccharolobus solfataricus	4-29
1883632-1	1991	The cytosolic isoenzyme of human liver alanine aminotransferase exhibited a progressive change in its chromatographic behavior on DEAE-Sepharose when partially purified preparations were stored for up to 8 days at 4 degrees C. This change was characterized by the appearance of an additional chromatographic variant and was avoided by addition of 2-mercaptoethanol.	deae-sepharose	130-144	glutamic--pyruvic transaminase	Homo sapiens	39-63
2170769-1	1990	Human placental 17 beta-hydroxysteroid dehydrogenase (17-HSD) was purified to apparent homogeneity using ammonium sulfate precipitation and chromatography on Red-Agarose and DEAE-Sepharose columns.	deae-sepharose	174-188	hydroxysteroid 17-beta dehydrogenase 1	Homo sapiens	6-52
2170769-1	1990	Human placental 17 beta-hydroxysteroid dehydrogenase (17-HSD) was purified to apparent homogeneity using ammonium sulfate precipitation and chromatography on Red-Agarose and DEAE-Sepharose columns.	deae-sepharose	174-188	hydroxysteroid 17-beta dehydrogenase 13	Homo sapiens	54-60
2445475-5	1987	gp90 was partially purified from a detergent-solubilized membrane preparation by chromatography on DEAE-Sepharose, hydroxylapatite, chelating Sepharose, and lectin-agarose columns.	deae-sepharose	99-113	galectin 3 binding protein	Homo sapiens	0-4
2288984-1	1990	A thiol-dependent serine proteinase has been isolated for the first time from a higher basidiomycete Coprinus 7N culture filtrate by affinity chromatography on bacitracin-Sepharose combined with ion-exchange chromatography on DEAE-Sepharose.	deae-sepharose	226-240	endogenous retrovirus group K member 19, envelope	Homo sapiens	25-35
2140256-1	1990	A tissue kallikrein has been isolated from rat heart extracts by DEAE-Sepharose and aprotinin-affinity column chromatography.	deae-sepharose	65-79	kallikrein 1-related peptidase C12	Rattus norvegicus	2-19
2303430-1	1990	A T-kininogenase has been purified to homogeneity from rat submandibular gland extracts by DEAE-Sepharose chromatography and preparative gel electrophoresis.	deae-sepharose	91-105	kallikrein 1-related peptidase C10	Rattus norvegicus	2-16
2337593-2	1990	Human placental NAD(+)-linked 15-hydroxyprostaglandin dehydrogenase was purified to homogeneity according to a five-step method, with chromatography on DEAE-Sepharose, Blue Sepharose, and Mono-Q FPLC as principal steps.	deae-sepharose	152-166	carbonyl reductase 1	Homo sapiens	30-67
2659722-1	1989	A chemotactic protein for polymorphonuclear leukocytes (lung carcinoma-derived chemotaxin [LUCT]) was purified from culture fluid of the human lung giant cell carcinoma LU65C cells to electrophoretically homogeneous form through five sequential purification steps: DEAE-Sepharose, CM-Sepharose, HPLC on carboxyl-methylated-polyvinylalcohol resin, hydrophobic, and reversed-phase.	deae-sepharose	265-279	C-X-C motif chemokine ligand 8	Homo sapiens	56-89
2659722-1	1989	A chemotactic protein for polymorphonuclear leukocytes (lung carcinoma-derived chemotaxin [LUCT]) was purified from culture fluid of the human lung giant cell carcinoma LU65C cells to electrophoretically homogeneous form through five sequential purification steps: DEAE-Sepharose, CM-Sepharose, HPLC on carboxyl-methylated-polyvinylalcohol resin, hydrophobic, and reversed-phase.	deae-sepharose	265-279	C-X-C motif chemokine ligand 8	Homo sapiens	91-95
3283124-4	1988	Fractionation showed that a DEAE-Sepharose fraction (BA) contained a novel factor, which specifically stimulated EGF receptor transcription 5- to 10-fold.	deae-sepharose	28-42	epidermal growth factor receptor	Homo sapiens	113-125
3499974-4	1987	This interleukin 1 (IL-1)-like activity exactly coeluted with BRA upon gel chromatography, DEAE-Sepharose ion-exchange chromatography, and isoelectric focusing (pI, 4.7-5.2).	deae-sepharose	91-105	interleukin 1 beta	Homo sapiens	5-24
2154223-1	1990	An extracellular myeloperoxidase precursor of HL-60 cells was purified from the culture supernatant by ammonium sulfate precipitation, DEAE-Sepharose chromatography, and monoclonal antibody affinity chromatography.	deae-sepharose	135-149	myeloperoxidase	Homo sapiens	17-32
2804057-6	1989	On DEAE-Sepharose, PLA2 eluted as two peaks, one in the flow-through fraction and the other with increased salt concentration.	deae-sepharose	3-17	phospholipase A2 group IB	Rattus norvegicus	19-23
2605669-4	1989	ODC activity induced by repetitive treatment with Co2+ was separated into two peaks by DEAE-Sepharose column chromatography.	deae-sepharose	87-101	ornithine decarboxylase 1	Rattus norvegicus	0-3
3147726-6	1988	Two forms of native LIF, distinguishable by their chromatographic behavior on DEAE-Sepharose, were converted by neuraminidase treatment to a form with similar chromatographic behavior, suggesting that the major difference between these two species is the content of sialic acid on the carbohydrate portion.	deae-sepharose	78-92	leukemia inhibitory factor	Mus musculus	20-23
3615421-1	1987	The highly active alcohol dehydrogenase (EC 1.1.1.1) in rat hepatic tumor cells (HTC) was purified 120-fold by chromatography on DEAE-Sepharose and AMP-Agarose to yield an enzyme with a specific activity of 88 mumole/min/mg protein, assayed with 1.7 mM NAD+ and 0.55 M ethanol at pH 9 and 30 degrees C. (By comparison, purified, normal rat liver enzyme has an activity of about 1 unit/mg.)	deae-sepharose	129-143	aldo-keto reductase family 1 member A1	Rattus norvegicus	18-39
3925940-1	1985	Ornithine decarboxylase isolated from HTC cells was separated into two distinct charged states by salt-gradient elution from DEAE-Sepharose columns.	deae-sepharose	125-139	ornithine decarboxylase 1	Rattus norvegicus	0-23
4065338-1	1985	Vimentin enriched in cytoskeletal frameworks by Triton X-100 extraction of Ehrlich ascites tumor cells and purified from a low ionic strength extract of the cell residues by (NH4)2SO4 precipitation and DEAE-Sepharose and ssDNA-cellulose chromatography in the presence of 6 M urea was highly contaminated with lipids.	deae-sepharose	202-216	vimentin	Mus musculus	0-8
4052013-3	1985	Undegraded and homogeneous carboxypeptidase A1 and A2 species (peptidyl-L-amino acid hydrolase, EC 3.4.17.1), in monomeric forms with high specific activity, are also obtained in high yield by controlled trypsin activation of either of the pro-(carboxypeptidases A) followed by chromatography on DEAE-Sepharose at pH 5.8 under dissociating conditions (7 M-urea).	deae-sepharose	296-310	carboxypeptidase A1	Sus scrofa	27-46
6607753-3	1984	DIF was purified 7,000-fold from HUT-102 conditioned media by utilizing ion-exchange chromatography with DEAE-Sepharose, gel chromatography, Blue-Sepharose chromatography, and preparative SDS-polyacrylamide gel electrophoresis (SDS-PAGE).	deae-sepharose	105-119	tumor necrosis factor	Homo sapiens	0-3
6525205-3	1984	Homogeneous catalase was obtained during DEAE-Sepharose C1-6B chromatography of monoamine oxidase.	deae-sepharose	41-55	catalase	Homo sapiens	12-20
6707018-1	1984	The activated glucocorticoid receptor (GR) from rat liver cytosol was purified by sequential chromatography on DNA-cellulose and DEAE-Sepharose.	deae-sepharose	129-143	nuclear receptor subfamily 3, group C, member 1	Rattus norvegicus	14-37
6707018-1	1984	The activated glucocorticoid receptor (GR) from rat liver cytosol was purified by sequential chromatography on DNA-cellulose and DEAE-Sepharose.	deae-sepharose	129-143	nuclear receptor subfamily 3, group C, member 1	Rattus norvegicus	39-41
3888276-8	1985	Studies with DEAE-Sepharose columns demonstrated that lecithin-cholesterol acyltransferase from these mammalian species was similar in molecular charge.	deae-sepharose	13-27	lecithin-cholesterol acyltransferase	Homo sapiens	54-90
6520123-1	1984	Spermidine synthase was purified to homogeneity from rat and pig liver by a method modified from a previously reported one using DEAE-Sepharose, S-adenosyl(5")-3-thiopropylamine-Sepharose affinity chromatography, Sephacryl S-300 gel filtration and polyacrylamide gel electrophoresis.	deae-sepharose	129-143	spermidine synthase	Rattus norvegicus	0-19
6301828-7	1983	Protein phosphatase-2B became activated 10-fold by calmodulin (A0.5 = 6 nM) after chromatography on DEAE-Sepharose and this degree of activation was maintained throughout the remainder of the purification.	deae-sepharose	100-114	calmodulin	Oryctolagus cuniculus	51-61
6553016-3	1983	Human salivary kallikrein was isolated from saliva using affinity chromatography on aprotinin-Sepharose and anti-human urinary kallikrein IgG-Sepharose followed by ion exchange chromatography on DEAE-Sepharose.	deae-sepharose	195-209	kallikrein related peptidase 4	Homo sapiens	15-25
7150562-1	1982	An actin-modulating protein has been purified from rabbit alveolar macrophages utilizing DEAE-Sepharose and gel filtration chromatography.	deae-sepharose	89-103	actin	Oryctolagus cuniculus	3-8
6572356-3	1983	DEAE-Sepharose column chromatographic patterns of 4-methylumbelliferyl sulfatases A, B1, and B2 in leukocytes and cultured skin fibroblasts from MSD patients and heterozygotes were also consistent with the above data.	deae-sepharose	0-14	membrane spanning 4-domains A1	Homo sapiens	85-95
6822511-10	1983	The two activities probably represent discrete enzymes since monoacylglycerol lipase activity can be separated from diacylglycerol lipase by fractionation on DEAE-Sepharose, although both are contained in the membrane fraction of platelets.	deae-sepharose	158-172	monoglyceride lipase	Homo sapiens	61-84
6856582-3	1983	This method, which involves affinity chromatography with heparin-Sepharose, ion exchange chromatography using DEAE-Sepharose, and Sephadex G-100 gel chromatography, results in purified, functionally active AT III with a 39% yield.	deae-sepharose	110-124	antithrombin-III	Cavia porcellus	206-212
6980938-5	1982	However, L-TCGF is a more acidic (pI 4.5 vs 6.5 to 8.0) molecule than N-TCGF and elutes from DEAE-Sepharose at higher salt concentration (0.2 M NaCl vs 0.07 to 0.1 M NaCl).	deae-sepharose	93-107	interleukin 2	Homo sapiens	9-15
7174639-1	1982	An aminopeptidase was purified about 700-fold from porcine liver homogenate by ammonium sulfate fractionation and a series of column chromatographies on DEAE-cellulose, Sephadex G-150, DEAE-Sepharose, and hydroxyapatite.	deae-sepharose	185-199	carboxypeptidase Q	Homo sapiens	3-17
6980938-5	1982	However, L-TCGF is a more acidic (pI 4.5 vs 6.5 to 8.0) molecule than N-TCGF and elutes from DEAE-Sepharose at higher salt concentration (0.2 M NaCl vs 0.07 to 0.1 M NaCl).	deae-sepharose	93-107	interleukin 2	Homo sapiens	11-15
6895893-3	1982	The inhibitor, designated heparin cofactor II (HCII), was purified to homogeneity with sulfated-dextran, DEAE-Sepharose, heparin-Sepharose and Sephadex G-150.	deae-sepharose	105-119	serpin family D member 1	Homo sapiens	26-45
7150233-1	1982	Complement component C3b has been purified from human, rabbit and bovine serum by affinity chromatography on human factor H-Sepharose after preliminary fractionation by poly(ethylene glycol) and DEAE-Sepharose.	deae-sepharose	195-209	complement C3	Homo sapiens	0-24
6895893-3	1982	The inhibitor, designated heparin cofactor II (HCII), was purified to homogeneity with sulfated-dextran, DEAE-Sepharose, heparin-Sepharose and Sephadex G-150.	deae-sepharose	105-119	serpin family D member 1	Homo sapiens	47-51
7332869-5	1981	Two peaks of pure transferrin were obtained by DEAE-Sepharose chromatography.	deae-sepharose	47-61	transferrin	Mus musculus	18-29
6976987-1	1982	Human T cell growth factor (TCGF) has been purified more than 800-fold from serum-free lymphocyte conditioned media by utilizing ion exchange chromatography with DEAE-Sepharose, gel filtration with Ultrogel AcA54, and preparative SDS-polyacrylamide gel electrophoresis.	deae-sepharose	162-176	interleukin 2	Homo sapiens	28-32
6905639-1	1980	Human urinary kallikrein was purified by gel filtration on Sephacryl S-200 and affinity chromatography on aprotinin-Sepharose, followed by ion exchange chromatography on DEAE-Sepharose.	deae-sepharose	170-184	kallikrein related peptidase 4	Homo sapiens	14-24
7194873-2	1981	Androgen-binding protein (ABP) was purified from caput epididymis of the rat by sequential chromatography on DEAE-Sepharose, hydroxylapatite, dihydrotestosterone-17 beta-hemisuccinyl-1,6-diaminohexane-Sepharose, and Sephadex G-150.	deae-sepharose	109-123	sex hormone binding globulin	Rattus norvegicus	0-24
7194873-2	1981	Androgen-binding protein (ABP) was purified from caput epididymis of the rat by sequential chromatography on DEAE-Sepharose, hydroxylapatite, dihydrotestosterone-17 beta-hemisuccinyl-1,6-diaminohexane-Sepharose, and Sephadex G-150.	deae-sepharose	109-123	sex hormone binding globulin	Rattus norvegicus	26-29
7032172-5	1981	beta-Lactamase was purified to homogeneity from shock fluid by a one-step procedure in a DEAE-Sepharose column.	deae-sepharose	89-103	beta-lactamase	Escherichia coli	0-14
7451402-1	1980	An aminopeptidase B from porcine liver was purified about 2,000-fold by ammonium sulfate fractionation and a series of chromatographies on hydroxyapatite, DEAE-cellulose, Sephadex G-150, hydroxyapatite and DEAE-Sepharose columns.	deae-sepharose	206-220	arginyl aminopeptidase	Homo sapiens	3-19
7383973-2	1980	Rat ceruloplasmin was purified by a three-step column chromatography procedure, utilizing DEAE-Sepharose, Sepharose CL-6B, and CM-Sephadex A50 columns.	deae-sepharose	90-104	ceruloplasmin	Rattus norvegicus	4-17
6157632-1	1980	Human urinary kallikrein was purified by gel filtration on Sephacryl S-200 and affinity chromatography on aprotinin-Sepharose, followed by ion exchange chromatography on DEAE-Sepharose.	deae-sepharose	170-184	kallikrein related peptidase 4	Homo sapiens	14-24
575476-1	1979	An active preparation of lecithin: cholesterol acyltransferase (LCAT, EC 2.3.1.43) was isolated from human plasma by density ultracentrifugation, high-density lipoprotein affinity chromatography, DEAE-Sepharose and hydroxylapatite chromatography.	deae-sepharose	196-210	lecithin-cholesterol acyltransferase	Homo sapiens	25-62
575476-1	1979	An active preparation of lecithin: cholesterol acyltransferase (LCAT, EC 2.3.1.43) was isolated from human plasma by density ultracentrifugation, high-density lipoprotein affinity chromatography, DEAE-Sepharose and hydroxylapatite chromatography.	deae-sepharose	196-210	lecithin-cholesterol acyltransferase	Homo sapiens	64-68
312799-6	1979	Analysis of the DEAE-Sepharose elution profiles indicated that there were two major charge species of GM-CSF eluting at conductivities of 10 and 14 mmho.	deae-sepharose	16-30	colony stimulating factor 2 (granulocyte-macrophage)	Mus musculus	102-108