PMID-sentid Pub_year Sent_text compound_name comp_offset prot_official_name organism prot_offset 33161753-3 2021 Our studies showed that rapid filling of a Mag-Fura-2-sensitive free Ca2+ pool during SERCA1-mediated Ca2+ sequestration appears to be a crucial condition allowing RyR1 Ca2+ channels to close once reloading of luminal Ca2+ stores is complete. Fura-2 47-53 ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 Homo sapiens 86-92 32740939-5 2021 The study used the mouse GT1-7 cell line as a model system, and Fura-2 based calcium imaging for detecting the interruption of intracellular calcium transport by the extracellular presence of Gd3+ as released from the CAs; gadodiamide and gadoterate meglumine, when the cells were stimulated in vitro culture by exposure to melatonin.The CA gadoterate meglumine interfered minimally with the calcium signaling, and thus its use is preferable in standard MRI exams. Fura-2 64-70 GRDX Homo sapiens 192-195 33161753-3 2021 Our studies showed that rapid filling of a Mag-Fura-2-sensitive free Ca2+ pool during SERCA1-mediated Ca2+ sequestration appears to be a crucial condition allowing RyR1 Ca2+ channels to close once reloading of luminal Ca2+ stores is complete. Fura-2 47-53 ryanodine receptor 1 Homo sapiens 164-168 33310045-7 2021 Functional TRPM7 in MDA-MB-231, MCF-7, and HEK293 cells were tested with western blotting, patch-clamp, and fura-2 quench assay. Fura-2 108-114 transient receptor potential cation channel subfamily M member 7 Homo sapiens 11-16 32594194-9 2020 This Ca2+ entry induced by haloperidol was confirmed by Mn2+ entry-induced quench of fura-2 fluorescence. Fura-2 85-91 carbonic anhydrase 2 Rattus norvegicus 5-8 33255426-9 2020 Fura-2 imaging and extracellular Ca2+ withdrawal confirmed cytosolic Ca2+ transients as a contributing factor of neurotransmitter-induced redox responses and their potentiation in the cytosol of MeCP2-deficient neurons. Fura-2 0-6 methyl CpG binding protein 2 Mus musculus 195-200 33166100-6 2020 We applied pharmacological as well as CRISPR/Cas9-based strategies to analyse the interplay of TRPM3 and voltage-gated Ca2+ channels in imaging experiments (FMP, Fura-2) and electrophysiological recordings. Fura-2 162-168 transient receptor potential cation channel, subfamily M, member 3 Rattus norvegicus 95-100 30914247-8 2019 Antimycin A-evoked activation of vagal nociceptors in a Fura2 Ca2+ assay correlated with TRPV1 responses compared to TRPA1 responses. Fura-2 56-61 transient receptor potential cation channel subfamily V member 1 Homo sapiens 89-94 32047108-6 2020 Using bacterial cells loaded with the fluorescent Fura-2 probe, we further obtained direct biochemical evidence that TMEM165 mediates Ca2+ and Mn2+ influxes. Fura-2 50-56 transmembrane protein 165 Homo sapiens 117-124 32082084-7 2020 However, fura-2-based fluorescence imaging showed calcium signals upon application of arginine vasopressin or ghrelin within intact pancreatic islets. Fura-2 9-15 ghrelin Mus musculus 110-117 31586031-5 2019 The results of a Fura-2 intracellular Ca2+ assay validated Piezo1 as a functional ion channel that is activated by its agonist, Yoda1. Fura-2 17-23 piezo-type mechanosensitive ion channel component 1 Mus musculus 59-65 31498851-3 2019 METHODS: Using epifluorescence microscopy, we monitored relative changes in the cytosolic fluorescence intensity of Fura2 as a marker of change in intracellular Ca2+ ([Ca2+]i) and relative increases in green fluorescent protein (GFP)-tagged myristoylated alanine-rich C kinase substrate (MARCKS-GFP) as a marker of PKC activation in response to GW9508 at 3 mM and 20 mM glucose. Fura-2 116-121 myristoylated alanine rich protein kinase C substrate Rattus norvegicus 241-286 31498851-3 2019 METHODS: Using epifluorescence microscopy, we monitored relative changes in the cytosolic fluorescence intensity of Fura2 as a marker of change in intracellular Ca2+ ([Ca2+]i) and relative increases in green fluorescent protein (GFP)-tagged myristoylated alanine-rich C kinase substrate (MARCKS-GFP) as a marker of PKC activation in response to GW9508 at 3 mM and 20 mM glucose. Fura-2 116-121 myristoylated alanine rich protein kinase C substrate Rattus norvegicus 288-294 31498851-3 2019 METHODS: Using epifluorescence microscopy, we monitored relative changes in the cytosolic fluorescence intensity of Fura2 as a marker of change in intracellular Ca2+ ([Ca2+]i) and relative increases in green fluorescent protein (GFP)-tagged myristoylated alanine-rich C kinase substrate (MARCKS-GFP) as a marker of PKC activation in response to GW9508 at 3 mM and 20 mM glucose. Fura-2 116-121 protein kinase C, alpha Rattus norvegicus 315-318 31067348-5 2019 Intracellular calcium oscillations of BMMs with RANKL were detected by staining with calcium indicator fura-2 immediately after administration of CTLA4-Ig or after one day of treatment. Fura-2 103-109 tumor necrosis factor (ligand) superfamily, member 11 Mus musculus 48-53 30508321-6 2019 Based on cobalt mediated quenching effects, calcium imaging experiments using fura-2 showed a concentration-dependent cobalt influx in MG-63 cells, which could be inhibited by the unspecific TRPM7 channel inhibitor 2-APB (20 muM) and TRPM7 specific siRNA. Fura-2 78-84 transient receptor potential cation channel subfamily M member 7 Homo sapiens 191-196 30508321-6 2019 Based on cobalt mediated quenching effects, calcium imaging experiments using fura-2 showed a concentration-dependent cobalt influx in MG-63 cells, which could be inhibited by the unspecific TRPM7 channel inhibitor 2-APB (20 muM) and TRPM7 specific siRNA. Fura-2 78-84 transient receptor potential cation channel subfamily M member 7 Homo sapiens 234-239 32540611-4 2020 MATERIALS AND METHODS: In human HNSCC cell lines CAL27 and FaDu and the mouse oral cancer cell line MOC2, P2Y2R contributions to extracellular nucleotide-induced changes in intracellular free Ca2+ concentration and EGFR and extracellular signal-regulated kinase (ERK1/2) phosphorylation were determined using the ratiometric Ca2+ indicator fura-2 and immunoblot analysis, respectively. Fura-2 340-346 purinergic receptor P2Y2 Homo sapiens 106-111 32466589-4 2020 Therefore, in the present work, the possibility of bioluminescence-driven miniSOG photodynamic CCK1R activation was examined, as monitored by Fura-2 calcium imaging. Fura-2 142-148 cholecystokinin A receptor Rattus norvegicus 95-100 32322744-5 2020 Compared to NT, PRL (50 ng/ml) significantly increased the amplitude of Ca2+ transients in myocytes from control (0.084 +- 0.004 vs. 0.115 +- 0.007 Fura-2 ratio units) and diabetic (0.087 +- 0.007 vs. 0.112 +- 0.006 Fura-2 ratio units) heart (n = 36-50 cells). Fura-2 148-154 prolactin Rattus norvegicus 16-19 32322744-5 2020 Compared to NT, PRL (50 ng/ml) significantly increased the amplitude of Ca2+ transients in myocytes from control (0.084 +- 0.004 vs. 0.115 +- 0.007 Fura-2 ratio units) and diabetic (0.087 +- 0.007 vs. 0.112 +- 0.006 Fura-2 ratio units) heart (n = 36-50 cells). Fura-2 216-222 prolactin Rattus norvegicus 16-19 31805819-11 2020 Ca2+ measurements using the cytosolic indicator Fura-2 and the endoplasmic reticulum luminal store indicator D1ER suggest that TRIC-A enhances Ca2+ leak across the endoplasmic reticulum by directly targeting RyR2 to modulate store overload-induced Ca2+ release. Fura-2 48-54 transmembrane protein 38A Homo sapiens 127-133 31834667-6 2020 5-Oxazolecarboxylic acid senses Mg2+ ions, exhibiting a sensitivity of 0.10-0.44 mmol L-1 within the range of 0.5-0.8 mmol L-1 . Fura-2 0-24 L1 cell adhesion molecule Homo sapiens 86-89 31834667-6 2020 5-Oxazolecarboxylic acid senses Mg2+ ions, exhibiting a sensitivity of 0.10-0.44 mmol L-1 within the range of 0.5-0.8 mmol L-1 . Fura-2 0-24 L1 cell adhesion molecule Homo sapiens 123-126 31444738-2 2019 Fura-2-loaded HEK293-P2X2-PROG cells were acutely plated on top of cultured DH glial cells to quantify ATP release from single DH glial cells. Fura-2 0-6 purinergic receptor P2X 2 Homo sapiens 21-25 29632074-5 2018 Here, we produced the yeast UPF0016 member Gdt1p in the bacterial host Lactococcus lactis Using Mn2+-induced quenching of Fura-2-emitted fluorescence, we observed that Gdt1p mediates Mn2+ influx, in addition to its previously reported regulation of Ca2+ influx. Fura-2 122-128 putative ribosome biosynthesis protein GDT1 Saccharomyces cerevisiae S288C 43-48 30139243-8 2018 Mn2+ has been shown to enter cellsthrough similar mechanisms as Ca2+ but quenches fura-2 fluorescence at all excitation wavelengths.Captopril (3000 muM)-induced Mn2+ influx indirectly suggested that captopril evoked Ca2+ entry.Captopril-induced Ca2+ entry was inhibited by 15% by a protein kinase C (PKC) activator (phorbol12-myristate 13 acetate, PMA) and an inhibitor (GF109203X) and three inhibitors of store-operatedCa2+ channels: nifedipine, econazole and SKF96365. Fura-2 82-88 latexin Homo sapiens 148-151 29632074-5 2018 Here, we produced the yeast UPF0016 member Gdt1p in the bacterial host Lactococcus lactis Using Mn2+-induced quenching of Fura-2-emitted fluorescence, we observed that Gdt1p mediates Mn2+ influx, in addition to its previously reported regulation of Ca2+ influx. Fura-2 122-128 putative ribosome biosynthesis protein GDT1 Saccharomyces cerevisiae S288C 168-173 29867546-4 2018 Cold light irradiation (87 mW cm-2) was applied to photosensitizer-expressing cells to examine photodynamic activation of CCK1R by Fura-2 fluorescent calcium imaging. Fura-2 131-137 cholecystokinin A receptor Homo sapiens 122-127 28771237-5 2017 [Ca2+]i measurements are most commonly performed in intact single fibers using ratiometric fluorescent indicators such as indo-1 or fura-2. Fura-2 132-138 carbonic anhydrase 2 Homo sapiens 1-4 29531809-7 2018 Fura-2 measurements on single nuclei and patch-clamp experiments in whole-cell configuration showed that XIP-NLS selectively inhibited nuNCX1. Fura-2 0-6 late endosomal/lysosomal adaptor, MAPK and MTOR activator 5 Homo sapiens 105-108 28939258-9 2017 Fura-2 imaging was used to also address acute insulin effects on spontaneous and depolarization-mediated Ca2+ transients. Fura-2 0-6 insulin Homo sapiens 46-53 28912066-3 2017 cAMP-elevating agents (forskolin and dibutyryl-cAMP) and the joint activation of PKA (6-Bnz-cAMP) and Epac (8-pCPT-2"-O-Me-cAMP) increased cytoplasmic Ca2+ concentration ([Ca2+]c) in <=30% of fura-2-loaded isolated human umbilical vein endothelial cells (HUVEC). Fura-2 195-201 Rap guanine nucleotide exchange factor 3 Homo sapiens 102-106 28233567-5 2017 Disparity in regulation has been previously reported among the splice variants of NCX3 therefore the activity of Ca2+ uptake and extrusion of the two murine variants was measured using fura-2-based Ca2+ imaging and revealed that both variants are similarly regulated. Fura-2 185-191 solute carrier family 8 (sodium/calcium exchanger), member 3 Mus musculus 82-86 27443158-6 2017 In whole-cell patch-clamp and intracellular-free calcium ([Ca2+]) concentration (Fura-2) experiments, cumene hydroperoxide and ADP-ribose-induced TRPM2 currents in the neurons were inhibited by N-(p-amylcinnamoyl) anthranilic acid (ACA) and capsaicin-induced TRPV1 currents were inhibited by capsazepine (CPZ) incubations. Fura-2 81-87 transient receptor potential cation channel, subfamily M, member 2 Rattus norvegicus 146-151 28052871-13 2017 Myoglobin increased the fura 2 fluorescence ratio (cytosolic calcium) during ANG II application (10-12 to 10-6 M). Fura-2 24-30 myoglobin Mus musculus 0-9 29043988-3 2017 Concentration of Ca2+ in hippocampus was determined by Fura-2/AM fluorescence probe and the expression of Calbindin-D28K (CB) in hippocampal CA1 was tested by immunohistochemistry. Fura-2 55-61 carbonic anhydrase 2 Rattus norvegicus 17-20 28052871-13 2017 Myoglobin increased the fura 2 fluorescence ratio (cytosolic calcium) during ANG II application (10-12 to 10-6 M). Fura-2 24-30 angiotensinogen (serpin peptidase inhibitor, clade A, member 8) Mus musculus 77-83 26990768-2 2017 METHODS: Fura-2-loaded HEK-293 cells transfected with the histamine H1 receptor was used as a sensitive biosensor assay for histamine release from isolated mouse mesenteric arteries. Fura-2 9-15 histamine receptor H1 Homo sapiens 58-79 27252039-5 2016 Protriptyline-induced Ca2+ entry was confirmed by Mn2+ -induced quench of fura-2 fluorescence. Fura-2 74-80 carbonic anhydrase 2 Homo sapiens 22-25 27638877-9 2016 Ca2+ imaging of endothelial cells and rat mesenteric resistance arteries using fura-2, revealed that both GLP-1 analogs caused a reduction in VEGFA-induced Ca2+ signaling. Fura-2 79-85 glucagon Rattus norvegicus 106-111 26450366-4 2016 EXPERIMENTAL APPROACH: Thrombin-evoked Ca(2+) fluxes were monitored in Fura-2- or Fluo-5N-loaded human platelets, or using platelet suspensions containing Fluo-4 or Rhod-5N K(+) salts. Fura-2 71-77 coagulation factor II, thrombin Homo sapiens 23-31 27371735-6 2016 Measuring intracellular Ca(2+) flux in Fura-2-loaded HASM cultures, we observed that TNF exposure enhanced cytosolic Ca(2+) mobilization and changed the temporal pattern of Ca(2+) flux in individual myocytes induced by NMDA, an NMDA-R selective analog of glutamate. Fura-2 39-45 tumor necrosis factor Mus musculus 85-88 26838264-6 2016 Intracellular dialysis of NAADP into Fura-2-loaded DT40TKO-hTPC2 cells elicited cytosolic Ca(2+) transients, suggesting that hTPC2 was functionally active. Fura-2 37-43 two pore segment channel 2 Homo sapiens 59-64 26838264-6 2016 Intracellular dialysis of NAADP into Fura-2-loaded DT40TKO-hTPC2 cells elicited cytosolic Ca(2+) transients, suggesting that hTPC2 was functionally active. Fura-2 37-43 two pore segment channel 2 Homo sapiens 125-130 27064319-4 2016 In order to detect the TRPV1-mediated responses in cultured DRG neurons, we monitored intracellular calcium responses to capsaicin, a TRPV1 agonist, with Fura-2. Fura-2 154-160 transient receptor potential cation channel, subfamily V, member 1 Mus musculus 23-28 25805851-3 2016 Fura-2 loaded cells isolated from young (3-4 months old) and aged (24 months old) mouse responded to acetylcholine (ACh) and cholecystokinin (CCK) with a polarized Ca(2+) response initiated at the secretory pole before spreading to the basal one. Fura-2 0-6 cholecystokinin Mus musculus 125-140 25805851-3 2016 Fura-2 loaded cells isolated from young (3-4 months old) and aged (24 months old) mouse responded to acetylcholine (ACh) and cholecystokinin (CCK) with a polarized Ca(2+) response initiated at the secretory pole before spreading to the basal one. Fura-2 0-6 cholecystokinin Mus musculus 142-145 27185316-5 2016 The interactions of CSQ1/STIM1 and STIM1/Orai1, and internal Ca2+ changes were detected by co-immunoprecipitation and Fura2, respectively. Fura-2 118-123 calsequestrin 1 Homo sapiens 20-24 27185316-5 2016 The interactions of CSQ1/STIM1 and STIM1/Orai1, and internal Ca2+ changes were detected by co-immunoprecipitation and Fura2, respectively. Fura-2 118-123 stromal interaction molecule 1 Homo sapiens 35-40 26434503-9 2015 Thrombin-evoked rises in [Na(+)]cyt were potentiated by PKC inhibition, an effect which was not due to altered changes in non-selective cation permeability of the plasma membrane as assessed by Mn(2+) quench of Fura-2 fluorescence. Fura-2 211-217 coagulation factor II, thrombin Homo sapiens 0-8 26427796-5 2015 The degree of platelet-derived growth factor-BB-induced increase in intracellular Ca(2+) was smaller in Fura2-labeled Epac1(-/-)-ASMCs than in Epac1(+/+)-ASMCs. Fura-2 104-109 Rap guanine nucleotide exchange factor (GEF) 3 Mus musculus 118-123 26047504-5 2015 Functional TRPV4 activity was assessed in cultured RMECs from endothelial Ca2+-responses recorded using fura-2 microfluorimetry and electrophysiological recordings of membrane currents. Fura-2 104-110 transient receptor potential cation channel, subfamily V, member 4 Rattus norvegicus 11-16 25324545-5 2014 Treatment of C5a with PPAD in vitro resulted in decreased chemotaxis of human neutrophils and diminished calcium signaling in monocytic cell line U937 transfected with the C5a receptor (C5aR) and loaded with a fluorescent intracellular calcium probe: Fura-2 AM. Fura-2 251-257 complement C5a receptor 1 Homo sapiens 13-16 26053751-4 2015 However, red-shifted versions with green and orange or red FPs (GFP, OFP, RFP) have some advantages such as less phototoxicity and minimal spectral overlay with autofluorescence of cells and fura-2, a prominent chemical Ca2+ indicator. Fura-2 191-197 tripartite motif containing 27 Homo sapiens 74-77 25767275-11 2015 Imaging of fura-2-loaded SMCs revealed that SMC assemblies displayed coordinated calcium oscillations and cell-cell transmission of calcium waves which, together with increased connexin 43-containing junctions, depended on cadherin-11 and N-cadherin function. Fura-2 11-17 gap junction protein alpha 1 Homo sapiens 177-188 25767275-11 2015 Imaging of fura-2-loaded SMCs revealed that SMC assemblies displayed coordinated calcium oscillations and cell-cell transmission of calcium waves which, together with increased connexin 43-containing junctions, depended on cadherin-11 and N-cadherin function. Fura-2 11-17 cadherin 11 Homo sapiens 223-234 25767275-11 2015 Imaging of fura-2-loaded SMCs revealed that SMC assemblies displayed coordinated calcium oscillations and cell-cell transmission of calcium waves which, together with increased connexin 43-containing junctions, depended on cadherin-11 and N-cadherin function. Fura-2 11-17 cadherin 2 Homo sapiens 239-249 25798335-5 2015 METHODS: As PMCA4 removes cytosolic calcium, we measured transient changes and the time-dependent decay of cytosolic calcium level as visualized by using fura-2 fluorescent dye with confocal microscopy in human SH-SY5Y neuroblastoma cells overexpressing either wild-type or R268Q mutant PMCA4. Fura-2 154-160 ATPase plasma membrane Ca2+ transporting 4 Homo sapiens 12-17 25395620-7 2015 Furthermore, calcium imaging experiments were performed with fura-2-loaded human embryonic kidney 293 cells overexpressing CysLT1Rs and with isolated mesenteric vascular smooth muscle cells. Fura-2 61-67 cysteinyl leukotriene receptor 1 Homo sapiens 123-129 24488794-3 2014 The staining method, Fura 2 stain, has sensitivities of 16-32 ng of alpha-casein and beta-casein, 62 ng of ovalbumin, phosvitin, and kappa-casein using an ultraviolet transilluminator. Fura-2 21-27 casein beta Homo sapiens 85-96 24471656-5 2014 In enzymatically dissociated human ASM cells loaded with the Ca(2+) indicator, fura-2, AA (1-10 muM) triggered [Ca(2+)]i oscillations that were inhibited by removal of extracellular Ca(2+). Fura-2 79-85 H19 imprinted maternally expressed transcript Homo sapiens 35-38 24690333-6 2014 Using video-microscopy to monitor cell contraction, relaxation, and intracellular Ca(2+) transients (Fura-2), we report that incorporation of L48Q cTnC significantly increased contractility of cardiomyocytes from healthy and MI hearts without adversely affecting Ca(2+) transient properties or relaxation. Fura-2 101-107 troponin C1, slow skeletal and cardiac type Homo sapiens 147-151 24101294-9 2014 This notion was supported by examining fura-2-loaded isolated AT1A (-/-) and AT1B (-/-) VSMCs. Fura-2 39-45 angiotensin II receptor, type 1a Mus musculus 62-66 25239455-3 2014 In retrogradely labelled neurons from the glabrous skin of adult male Sprague-Dawley rats, NCX activity, as assessed with fura-2-based microfluorimetry, was only detected in putative nociceptive IB4+ neurons. Fura-2 122-128 solute carrier family 8 member A1 Rattus norvegicus 91-94 25080489-7 2014 Both ANG II and the AT1 agonist L162,313 increased the intracellular calcium transients, measured by fura-2, with a two-step pattern. Fura-2 101-107 angiotensinogen (serpin peptidase inhibitor, clade A, member 8) Mus musculus 5-11 25080489-7 2014 Both ANG II and the AT1 agonist L162,313 increased the intracellular calcium transients, measured by fura-2, with a two-step pattern. Fura-2 101-107 angiotensin II receptor, type 1a Mus musculus 20-23 24951051-4 2014 In the present study, based on the 3D crystal structure of the ZnT-1 homologue, YiiP, that predicts a homodimer that utilizes the H(+) electrochemical gradient to facilitate Zn(2+) efflux, we demonstrate ZnT-1 dependent Zn(2+) efflux from HEK 293T cells using FluoZin-3 and Fura 2 by single cell microscope based fluorescent imaging. Fura-2 274-280 solute carrier family 30 member 1 Homo sapiens 63-68 24951051-4 2014 In the present study, based on the 3D crystal structure of the ZnT-1 homologue, YiiP, that predicts a homodimer that utilizes the H(+) electrochemical gradient to facilitate Zn(2+) efflux, we demonstrate ZnT-1 dependent Zn(2+) efflux from HEK 293T cells using FluoZin-3 and Fura 2 by single cell microscope based fluorescent imaging. Fura-2 274-280 solute carrier family 30 member 1 Homo sapiens 204-209 24993595-10 2014 Furthermore, Fura-2 measurement and immuno-blotting analysis, respectively, revealed that PR3 stimulated small intracellular Ca(2+) mobilization and Thr696-specific phosphorylation of the myosin phosphatase target subunit 1 (MYPT1). Fura-2 13-19 proteinase 3 Homo sapiens 90-93 24993595-10 2014 Furthermore, Fura-2 measurement and immuno-blotting analysis, respectively, revealed that PR3 stimulated small intracellular Ca(2+) mobilization and Thr696-specific phosphorylation of the myosin phosphatase target subunit 1 (MYPT1). Fura-2 13-19 protein phosphatase 1 regulatory subunit 12A Homo sapiens 188-223 24828496-9 2014 Fura-2 experiments demonstrated increased activity of TRPV5 in response to dobutamine, which could be prevented by the PKA inhibitor H89. Fura-2 0-6 transient receptor potential cation channel subfamily V member 5 Homo sapiens 54-59 24488794-3 2014 The staining method, Fura 2 stain, has sensitivities of 16-32 ng of alpha-casein and beta-casein, 62 ng of ovalbumin, phosvitin, and kappa-casein using an ultraviolet transilluminator. Fura-2 21-27 casein kinase 2 beta Homo sapiens 118-127 24043696-8 2014 To this end, Orai1 protein abundance was determined by western blotting and SOCE by fura-2 fluorescence. Fura-2 84-90 ORAI calcium release-activated calcium modulator 1 Homo sapiens 13-18 24685970-12 2014 Use of PTHrP (0.5 mumol/L) also decreased the fluorescence intensity of the indicator for intracellular calcium, Fura-2AM (a membrane-permeable derivative of Fura 2). Fura-2 158-164 parathyroid hormone-like hormone Rattus norvegicus 7-12 23831210-5 2013 Here, we show by Fura2 Ca-imaging that pharmacological inhibition of TRPM4 in HL-1 mouse cardiac myocytes by 9-phenanthrol (10 muM) and flufenamic acid (10 and 100 muM) decreases Ca2+ oscillations followed by an overall increase in [Ca2+]i. Fura-2 17-22 transient receptor potential cation channel, subfamily M, member 4 Mus musculus 69-74 24637628-3 2013 Wild-type or mutant TRPV4 transgenes are commonly expressed in cultured mammalian cells and examined by Fura-2 fluorometry and by electrodes. Fura-2 104-110 transient receptor potential cation channel subfamily V member 4 Homo sapiens 20-25 24157978-1 2013 We examined the endothelin-1 (ET-1)-induced increase in the intracellular free Ca(2+) concentration ([Ca(2+)]i) in fura-2-loaded rat pulmonary small arteries. Fura-2 115-121 endothelin 1 Rattus norvegicus 16-28 24157978-1 2013 We examined the endothelin-1 (ET-1)-induced increase in the intracellular free Ca(2+) concentration ([Ca(2+)]i) in fura-2-loaded rat pulmonary small arteries. Fura-2 115-121 endothelin 1 Rattus norvegicus 30-34 23968883-8 2013 We observed that both cadmium and zinc permeate hTRPV5 in ion imaging experiments using Fura-2 or Newport Green DCF. Fura-2 88-94 transient receptor potential cation channel subfamily V member 5 Homo sapiens 48-54 23807706-9 2013 Inositol 1,4,5-trisphosphate (IP3) receptors (IP3R) have been functionally demonstrated in submucosal neurons loaded with the Ca(2+)-sensitive fluorescent dye, fura-2. Fura-2 160-166 inositol 1,4,5-trisphosphate receptor, type 1 Rattus norvegicus 46-50 24036592-7 2014 Functionally, the [Ca(2+)]i response to exogenous angiotensin II was enhanced in fura-2-loaded glomus cells dissociated from hypoxic rats when compared with those of the normoxic control animals. Fura-2 81-87 angiotensinogen Rattus norvegicus 50-64 23624270-6 2013 Furthermore, we found that nNOS expression enhances the nicotine-induced increase in the intracellular concentration of Ca(2+), using the Ca(2+)-sensitive fluorescent probe Fura2. Fura-2 173-178 nitric oxide synthase 1 Rattus norvegicus 27-31 22492652-11 2012 TRPV4-mediated calcium entry was examined in Fura-2-loaded cultured lens epithelium. Fura-2 45-51 transient receptor potential cation channel subfamily V member 4 Homo sapiens 0-5 23219911-3 2013 Here we examine SERCA function in dissociated sensory neurons using Fura-2 fluorometry. Fura-2 68-74 ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 3 Homo sapiens 16-21 23345219-4 2013 Using simultaneous fura-2 Ca(2+) imaging and amphotericin B perforated patch-clamp electrophysiology, we find that bradykinin raises [Ca(2+)](i) and induces a biphasic voltage response. Fura-2 19-25 kininogen 1 Homo sapiens 115-125 23296601-4 2013 Here we describe a single-cell Ca(2+) imaging technique that was used to measure the intracellular Ca(2+) levels to examine the function of Ca(2+)-binding proteins, STIM1 and Calcium release-activated Calcium channel regulator 2A (CRACR2A), using ratiometric Ca(2+) dye Fura-2 in adherent and non-adherent cells. Fura-2 270-276 calcium release activated channel regulator 2A Homo sapiens 175-229 23131343-7 2013 The TRPC3 inhibitor, pyrazole-3, prevented ATP- and OAG-dependent transport of Mn(2+) from extracellular stores, demonstrated by a dramatic reduction in the rate of fluorescence quenching of Fura-2. Fura-2 191-197 transient receptor potential cation channel subfamily C member 3 Homo sapiens 4-9 23797607-4 2013 The Fura-2 Ca2+ imaging revealed that IL-1beta increased intracellular Ca2+ concentration even after removal of extracellular Ca2+, which was blocked by an inhibitor of inositol 1,4,5-trisphosphate receptors, 2-aminoethoxydiphenyl borate (2-APB, 1 muM). Fura-2 4-10 interleukin 1 beta Homo sapiens 38-46 23142606-9 2013 WIN reduced TRPV1-induced Ca(2+) transients in fura2-loaded HCEC whereas pertussis toxin (PTX) preincubation obviated suppression by WIN of such rises caused by capsaicin (CAP). Fura-2 47-52 transient receptor potential cation channel, subfamily V, member 1 Mus musculus 12-17 22897875-5 2012 In Fura-2 Ca(2+) imaging analysis, ghrelin administration increased the intracellular Ca(2+) concentration in approximately 50% of total isolated anterior pituitary cells, and 20% of these cells strongly responded to ghrelin. Fura-2 3-9 ghrelin and obestatin prepropeptide Rattus norvegicus 35-42 21993540-4 2012 Bcl-2 overexpressing and control cells were loaded with Fura 2/AM and stimulated with different drugs. Fura-2 56-62 BCL2, apoptosis regulator Rattus norvegicus 0-5 22134903-5 2012 P2X7R activation for 2 h did not cause cell death but resulted in a sustained reduction in ER Ca2+ pool size, as evidenced by a diminished cyclopiazonic acid-induced Ca(2+) discharge (fura 2 assay) and a lower fluorescent signal in cells loaded with Mag-fura 2 (ER-specific Ca(2+)-fluorescent dye). Fura-2 184-190 purinergic receptor P2X, ligand-gated ion channel, 7 Mus musculus 0-5 22134903-5 2012 P2X7R activation for 2 h did not cause cell death but resulted in a sustained reduction in ER Ca2+ pool size, as evidenced by a diminished cyclopiazonic acid-induced Ca(2+) discharge (fura 2 assay) and a lower fluorescent signal in cells loaded with Mag-fura 2 (ER-specific Ca(2+)-fluorescent dye). Fura-2 254-260 purinergic receptor P2X, ligand-gated ion channel, 7 Mus musculus 0-5 21873674-7 2011 ET-1-induced ONHA fura-2 ratio increases were significantly greater in TSL ONHAs (by 20% at 10-7 M and by 48% at 10-9 M) compared with WT ONHAs. Fura-2 18-24 endothelin 1 Rattus norvegicus 0-4 21803870-10 2011 In fura 2-loaded ASM cells, [Ca(2+)](i) responses to 1 muM ACh, 10 muM histamine, or 10 nM bradykinin were all exaggerated by TNF-alpha as well as IL-13 exposure. Fura-2 3-9 kininogen 1 Homo sapiens 91-101 21803870-10 2011 In fura 2-loaded ASM cells, [Ca(2+)](i) responses to 1 muM ACh, 10 muM histamine, or 10 nM bradykinin were all exaggerated by TNF-alpha as well as IL-13 exposure. Fura-2 3-9 tumor necrosis factor Homo sapiens 126-135 21803870-10 2011 In fura 2-loaded ASM cells, [Ca(2+)](i) responses to 1 muM ACh, 10 muM histamine, or 10 nM bradykinin were all exaggerated by TNF-alpha as well as IL-13 exposure. Fura-2 3-9 interleukin 13 Homo sapiens 147-152 23185465-4 2012 RT-PCR and Western blot found that the expression level of Orai1, the pore-forming unit of SOCE, was significantly elevated in the dystrophic muscles, while parallel increases in SOCE activity and SR Ca(2+) storage were detected in adult mdx muscles using Fura-2 fluorescence measurements. Fura-2 256-262 ORAI calcium release-activated calcium modulator 1 Mus musculus 59-64 22034516-8 2011 Furthermore, calcium imaging with Fura-2 showed that application of L-AP4, an agonist for group III metabotropic glutamate receptors including mGluR6, elevated calcium in endothelial cells. Fura-2 34-40 glutamate receptor, ionotropic, kainate 2 (beta 2) Mus musculus 143-149 21873674-8 2011 ET-1-induced fura-2 ratio increases were blocked after pretreatment with BQ-610 (ETA antagonist) in WT and TSL ONHAs, but not by BQ-788 (ETB antagonist) in WT ONHAs. Fura-2 13-19 endothelin 1 Rattus norvegicus 0-4 21873674-8 2011 ET-1-induced fura-2 ratio increases were blocked after pretreatment with BQ-610 (ETA antagonist) in WT and TSL ONHAs, but not by BQ-788 (ETB antagonist) in WT ONHAs. Fura-2 13-19 endothelin receptor type A Rattus norvegicus 81-84 21658429-3 2011 To clarify the pathway underlying AMPK-induced feeding, we monitored the activity of single ARC neurons by measuring cytosolic Ca(2+) concentration ([Ca(2+)](i)) with fura-2 fluorescence imaging. Fura-2 167-173 protein kinase AMP-activated catalytic subunit alpha 2 Rattus norvegicus 34-38 21810651-4 2011 Thrombin rapidly increased [Ca(2+)](i), as measured by fura-2 fluorescence ratio imaging, in wild-type but not Nox1 null SMCs. Fura-2 55-61 coagulation factor II Mus musculus 0-8 21336954-2 2011 Since Ca(2+) plays an important role in cell degeneration, [Ca(2+)]( i ) in alpha-synuclein-overexpressed human glioma cells was analyzed by Fura-2 fluorometry. Fura-2 141-147 synuclein alpha Homo sapiens 76-91 21632949-10 2011 Fura2 imaging provided evidence for functional Ca(v)1.3 channels in the LSO of wild-type mice. Fura-2 0-5 calcium channel, voltage-dependent, L type, alpha 1D subunit Mus musculus 47-55 21506114-6 2011 In fura2-loaded HCEC, a TRPV1-3 selective agonist, 100 microM 2-aminoethoxydiphenyl borate (2-APB), induced intracellular Ca(2+) transients and an increase in non-selective cation outward currents that were suppressed by ruthenium-red (RuR) (10-20 microM), a non-selective TRPV channel blocker. Fura-2 3-8 transient receptor potential cation channel subfamily V member 1 Homo sapiens 24-29 21338579-8 2011 Using the fluorescent dye Fura2, we showed in MCF7 that TGZ or Delta2-TGZ induced an immediate increase in cytosolic calcium which was required for ERK1/2 phosphorylation and EGR1 mRNA induction as demonstrated by calcium chelation experiments. Fura-2 26-31 mitogen-activated protein kinase 3 Homo sapiens 148-154 21338579-8 2011 Using the fluorescent dye Fura2, we showed in MCF7 that TGZ or Delta2-TGZ induced an immediate increase in cytosolic calcium which was required for ERK1/2 phosphorylation and EGR1 mRNA induction as demonstrated by calcium chelation experiments. Fura-2 26-31 early growth response 1 Homo sapiens 175-179 20678811-3 2010 CCL3 treatment also altered intracellular Ca(2+) dynamics and increased Ca(2+) levels in hippocampal neurons, measured by fura-2 imaging techniques. Fura-2 122-128 C-C motif chemokine ligand 3 Rattus norvegicus 0-4 21146870-6 2011 Live cell imaging experiments with Fura-2 and NewPort Green DCF showed that overexpression of human TRPV6 increased the permeability for Ca(2+), Ba(2+), Sr(2+), Mn(2+), Zn(2+), Cd(2+), and interestingly also for La(3+) and Gd(3+). Fura-2 35-41 transient receptor potential cation channel subfamily V member 6 Homo sapiens 100-105 21221778-4 2011 This study shows that treatment of the wild type HEK293 cells with low concentrations of carbachol (1-10 muM), an agonist of the muscarinic receptor, resulted in non-oscillated but sustained [Ca(2+)](i) increase by loading the cells with 1 muM fura2/AM. Fura-2 244-249 latexin Homo sapiens 105-108 21221778-5 2011 However, repetitive and long lasting [Ca(2+)](i) oscillations could be induced in 31.1% of the tested cells loaded with 0.1 muM fura2/AM. Fura-2 128-133 latexin Homo sapiens 124-127 21541286-8 2011 Endoplasmic reticulum (ER) store depletion by TG enhanced intracellular Ca(2+) levels in loaded with Fura-2 neurons transfected with YFP-STIM1 and ORAI1, but not with YFP-STIM2 and ORAI1, which correlated well with the number of complexes formed. Fura-2 101-107 stromal interaction molecule 1 Rattus norvegicus 137-142 21858195-8 2011 In fura-2 loaded human ASM cells, NCX-mediated inward Ca(2+) exchange as well as outward exchange (measured as rates of change in [Ca(2+)](i)) was elicited by altering extracellular Na(+) and Ca(2+) levels. Fura-2 3-9 H19 imprinted maternally expressed transcript Homo sapiens 23-26 21858195-8 2011 In fura-2 loaded human ASM cells, NCX-mediated inward Ca(2+) exchange as well as outward exchange (measured as rates of change in [Ca(2+)](i)) was elicited by altering extracellular Na(+) and Ca(2+) levels. Fura-2 3-9 T cell leukemia homeobox 2 Homo sapiens 34-37 21051874-3 2011 MATERIALS AND METHODS: Intracellular calcium concentration ([Ca(2+)](i)) was measured with the indicator dye Fura-2 in response to lysophosphatidic acid, thrombin and sphingosine-1-phosphate. Fura-2 109-115 coagulation factor II, thrombin Homo sapiens 154-162 20739625-5 2010 The nifedipine-insensitive transient rise in [Ca(2+)](i) and the increase in Mn(2+) quench of fura-2 fluorescence caused by CPA were both reduced in cells treated with Orai1 siRNA. Fura-2 94-100 ORAI calcium release-activated calcium modulator 1 Mus musculus 168-173 20592232-2 2010 METHODS: A method for labeling ganglion cell layer (GCL) neurons with the calcium indicator Fura-2 in flat-mounted retinas of adult rats was established. Fura-2 92-98 germ cell-less 1, spermatogenesis associated Rattus norvegicus 52-55 20592232-6 2010 The basal intracellular Ca(2+) level, as revealed by the Fura-2 ratio, was elevated in GCL neurons of laser-treated eyes compared with controls. Fura-2 57-63 germ cell-less 1, spermatogenesis associated Rattus norvegicus 87-90 20739625-7 2010 Moreover, overexpression of STIM1 enhanced the rise in [Ca(2+)](i) and the increase in Mn(2+) quench of fura-2 fluorescence caused by CPA, and these responses were reduced in cells treated with Orai1 siRNA. Fura-2 104-110 stromal interaction molecule 1 Mus musculus 28-33 20639579-7 2010 Functional analysis of TRPV1-I585V, using the Ca(2+)-sensitive dye fura-2 to measure intracellular [Ca(2+)] concentrations, revealed a decreased channel activity in response to two typical TRPV1 stimuli, heat and capsaicin. Fura-2 67-73 transient receptor potential cation channel subfamily V member 1 Homo sapiens 23-28 20802507-10 2010 The ADM-mediated effects were similar to that of forskolin, which activates adenylyl cyclase; additionally, while the PKA inhibitor H89 and Ca2(+) chelator Fura-2 blocked the effect of ADM. Fura-2 156-162 adrenomedullin Rattus norvegicus 4-7 20802507-10 2010 The ADM-mediated effects were similar to that of forskolin, which activates adenylyl cyclase; additionally, while the PKA inhibitor H89 and Ca2(+) chelator Fura-2 blocked the effect of ADM. Fura-2 156-162 adrenomedullin Rattus norvegicus 185-188 20434434-8 2010 Intracellular Ca(2+) in CA1 region was measured with fura-2 during preconditioning. Fura-2 53-59 carbonic anhydrase 1 Rattus norvegicus 24-27 20725140-4 2010 Using intact and isolated human hVECs and the Fura-2 calcium (Ca2+) measurement technique, we showed that ET-1 induced a dose-dependent increase of total intracellular free Ca2+, with an EC50 of 1.3 x 10-10 mol/L. Fura-2 46-52 endothelin 1 Homo sapiens 106-110 20670427-10 2010 Additionally, oxytocin increases cytosolic calcium levels in fura-2-loaded HASMCs that were enhanced in cells treated for 24 hr with IL-13. Fura-2 61-67 interleukin 13 Homo sapiens 133-138 20381461-6 2010 To test the CaSR functionality, HBEC loaded with fura-2 were stimulated with extracellular Ca(2+) ([Ca(2+)](out)) which resulted in a concentration-dependent intracellular Ca(2+) ([Ca(2+)](i)) increase (potency approximately 5.6mM [Ca(2+)](out)). Fura-2 49-55 calcium sensing receptor Homo sapiens 12-16 20714225-7 2010 Calcium ion influx through Ca(V)3.2 was monitored by observing fura-2 fluorescence. Fura-2 63-69 immunoglobulin lambda variable 7-43 Homo sapiens 27-35 20080989-8 2010 NO-, ANP-, and cGMP-mediated inhibition of the hormone-induced increase in intracellular calcium concentration measured by Fura2 was suppressed in IRAG-deficient VSMC. Fura-2 123-128 inositol 1,4,5-triphosphate receptor associated 1 Mus musculus 147-151 20413646-6 2010 The authors developed and optimized a fluorescent dye-based bioassay measuring the fluorescence quench of fura-2 by TRPM7-mediated Mn2+ influx in HEK293 cells that stably overexpress TRPM7. Fura-2 106-112 transient receptor potential cation channel subfamily M member 7 Homo sapiens 116-121 20413646-6 2010 The authors developed and optimized a fluorescent dye-based bioassay measuring the fluorescence quench of fura-2 by TRPM7-mediated Mn2+ influx in HEK293 cells that stably overexpress TRPM7. Fura-2 106-112 transient receptor potential cation channel subfamily M member 7 Homo sapiens 183-188 20229195-9 2010 Modulation of Ca(2+) signaling by hBD-2 was validated by Fura-2 measurements in human colon carcinoma HT29 cells. Fura-2 57-63 defensin beta 4A Homo sapiens 34-39 20237285-8 2010 Using the fura-2 calcium imaging technique, cultured cortical cells responded to GHRH by increasing intracellular calcium. Fura-2 10-16 growth hormone releasing hormone Rattus norvegicus 81-85 20307742-5 2010 Ca(2+) influx by reverse NCX activity was measured by fura-2 fluorescence. Fura-2 54-60 solute carrier family 8 member A1 Rattus norvegicus 25-28 20307742-10 2010 Fura-2 fluorescence measurement revealed that Ca(2+) influx by reverse NCX activity depended on extracellular Ca(2+) concentration, and that this influx was blocked by NCX inhibitor KB-R7943 in a concentration-dependent manner. Fura-2 0-6 solute carrier family 8 member A1 Rattus norvegicus 71-74 20307742-10 2010 Fura-2 fluorescence measurement revealed that Ca(2+) influx by reverse NCX activity depended on extracellular Ca(2+) concentration, and that this influx was blocked by NCX inhibitor KB-R7943 in a concentration-dependent manner. Fura-2 0-6 solute carrier family 8 member A1 Rattus norvegicus 168-171 20569277-7 2010 Defining the mechanism(s) by which Grp94 exerts its antioxidant defence, the determination of cytosolic calcium levels in C2C12 cells by fura-2 showed a significantly reduced amount of releasable calcium from intracellular stores, both in conditions of Grp94 overexpression and after curcumin pre-treatment. Fura-2 137-143 heat shock protein 90, beta (Grp94), member 1 Mus musculus 35-40 20197030-4 2010 Combined patch-clamp and Fura-2 fluorescence recordings revealed that with 2 mM extracellular Ca(2+), and at a membrane potential of -80 mV, approximately 17% of the inward TRPA1 current is carried by Ca(2+). Fura-2 25-31 transient receptor potential cation channel subfamily A member 1 Homo sapiens 173-178 21063097-10 2010 In K562 cells, G(2)/M cell cycle arrest was preceded by activation of TRPV5/6-like nonselective cation channels in the plasma membrane 1-5 h p.i., resulting in an elevated Ca(2+) entry as evident from fura-2 Ca(2+) imaging. Fura-2 201-207 transient receptor potential cation channel subfamily V member 5 Homo sapiens 70-77 19913094-6 2010 Measurements with fura-2-loaded cells in suspension revealed that resting [Ca2+]i was elevated and agonist-induced [Ca2+]i increases were augmented in S1P lyase-deficient MEFs both in the presence and absence of extracellular Ca2+. Fura-2 18-24 sphingosine-1-phosphate receptor 1 Mus musculus 151-154 19996302-5 2009 TRPV6 activity was measured with fluorescence microscopy using Fura-2. Fura-2 63-69 transient receptor potential cation channel subfamily V member 6 Homo sapiens 0-5 20118617-5 2010 Ca(2+) influx by Na(+)-Ca(2+) exchange, measured by fura-2 fluorescence, showed dependence on extracellular Ca(2+) concentration, and it was blocked by NCX inhibitors KB-R7943, SEA0400, and SN-6. Fura-2 52-58 solute carrier family 8 member A1 Rattus norvegicus 152-155 19695100-3 2009 RESULTS: Fluorescent imaging of Fura-2 loaded human SW982 synoviocytes reveals immediate and delayed cytosolic calcium oscillations elicited by (1) TRPV1 agonists capsaicin and resiniferatoxin (20-40% of cells), (2) moderate and noxious temperature change, and (3) osmotic stress TRPV4 activation (11.5% of cells). Fura-2 32-38 transient receptor potential cation channel subfamily V member 1 Homo sapiens 148-153 19695100-3 2009 RESULTS: Fluorescent imaging of Fura-2 loaded human SW982 synoviocytes reveals immediate and delayed cytosolic calcium oscillations elicited by (1) TRPV1 agonists capsaicin and resiniferatoxin (20-40% of cells), (2) moderate and noxious temperature change, and (3) osmotic stress TRPV4 activation (11.5% of cells). Fura-2 32-38 transient receptor potential cation channel subfamily V member 4 Homo sapiens 280-285 19491242-7 2009 Activation of Epac by 8-pCPT-2"-O-Me-cAMP caused a transient 171.0 +/- 18.0 nM (n = 5) increase in intracellular Ca2+ in Fura-2-loaded aortic myocytes, which persisted in the absence of extracellular Ca2+. Fura-2 121-127 Rap guanine nucleotide exchange factor 3 Rattus norvegicus 14-18 19386284-5 2009 In fura-2-loaded TRPC1 knockdown cells, despite the decreased TRPC1 levels, cyclopiazonic acid-induced Ca2+ entry and store-operated Ca2+ entry following Ca2+ addition were elevated by 77% and 135%, respectively. Fura-2 3-9 transient receptor potential cation channel, subfamily C, member 1 Rattus norvegicus 17-22 19386284-5 2009 In fura-2-loaded TRPC1 knockdown cells, despite the decreased TRPC1 levels, cyclopiazonic acid-induced Ca2+ entry and store-operated Ca2+ entry following Ca2+ addition were elevated by 77% and 135%, respectively. Fura-2 3-9 transient receptor potential cation channel, subfamily C, member 1 Rattus norvegicus 62-67 19523685-6 2009 Signal-regulated Ca(2+) entry (SRCE), defined as a stimulus- and extracellular Ca(2+)-dependent increase in [Ca(2+)](i), was measured in PHM1-41 cells treated with oxytocin (G-protein coupled receptor (GPCR)-stimulated), thapsigargin (store depletion-stimulated), and OAG (diacylglycerol-stimulated), using Fura-2. Fura-2 307-313 oxytocin/neurophysin I prepropeptide Homo sapiens 164-172 19307462-4 2009 In cultured human MCs, cell-attached patch clamp and fura-2 fluorescence measurements showed that single knockdown of either TRPC1 or TRPC4 significantly attenuated thapsigargin-induced membrane currents and Ca2+ entry as well as Ang II-induced channel activity. Fura-2 53-59 transient receptor potential cation channel subfamily C member 1 Homo sapiens 125-130 19487023-3 2009 Using Fura-2-loaded isometric rings of rabbit artery, we found that the Ca(2+)-independent phospholipase A(2) (iPLA(2)) inhibitor, bromoenol lactone (BEL), reduced the KCl-induced tonic but not early phasic phase of T and potentiated [Ca(2+)](i), reducing Ca(2+) sensitization. Fura-2 6-12 calcium-independent phospholipase A2-gamma Oryctolagus cuniculus 111-119 19625771-3 2009 Furthermore, ECCE, as measured by Mn(2+) quench of Fura-2, is reconstituted by expression of a mutant DHPR alpha(1S) subunit (SkEIIIK) thought to be impermeable to divalent cations. Fura-2 51-57 quinoid dihydropteridine reductase Homo sapiens 102-106 19307462-4 2009 In cultured human MCs, cell-attached patch clamp and fura-2 fluorescence measurements showed that single knockdown of either TRPC1 or TRPC4 significantly attenuated thapsigargin-induced membrane currents and Ca2+ entry as well as Ang II-induced channel activity. Fura-2 53-59 transient receptor potential cation channel subfamily C member 4 Homo sapiens 134-139 19007332-4 2009 The amount of Ca(2+) released from the ER by a TRPV1 agonist, measured using fura-2, was the same as that released by a SERCA (sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase) inhibitor, indicating that TRPV1 agonist-sensitive stores substantially overlap with SERCA inhibitor-sensitive stores. Fura-2 77-83 transient receptor potential cation channel subfamily V member 1 Homo sapiens 47-52 19357288-10 2009 Finally, fura-2 calcium imaging indicated that membrane cholesterol alterations might change the vulnerability of cells to Abeta insult by altering calcium influx. Fura-2 9-15 amyloid beta precursor protein Homo sapiens 123-128 19157541-6 2009 Furthermore, by patch-clamp and Fura-2-ratiometric measurements we demonstrate that activation of the CaR leads to elevated TRPV5-mediated currents and increases intracellular Ca(2+) concentrations in cells co-expressing TRPV5 and CaR. Fura-2 32-38 calcium sensing receptor Homo sapiens 102-105 19157541-6 2009 Furthermore, by patch-clamp and Fura-2-ratiometric measurements we demonstrate that activation of the CaR leads to elevated TRPV5-mediated currents and increases intracellular Ca(2+) concentrations in cells co-expressing TRPV5 and CaR. Fura-2 32-38 transient receptor potential cation channel subfamily V member 5 Homo sapiens 124-129 19332490-6 2009 The nifedipine-insensitive sustained rise in [Ca(2+)](i) and the increase in Mn(2+) quench of fura-2 fluorescence caused by CPA were both inhibited in cells pretreated with antibody raised against an extracellular epitope of TRPC1. Fura-2 94-100 transient receptor potential cation channel, subfamily C, member 1 Mus musculus 225-230 19332490-7 2009 Moreover, STIM1 siRNA reduced the rise in [Ca(2+)](i) and Mn(2+) quench of fura-2 fluorescence caused by CPA, whereas overexpression of STIM1 resulted in a marked increase in these responses. Fura-2 75-81 stromal interaction molecule 1 Mus musculus 10-15 19221709-7 2009 Activation of GPR-54 by kisspeptin also caused reversible increases in [Ca(2+)](i) in Fura-2 loaded dispersed islet cells. Fura-2 86-92 KISS1 receptor Mus musculus 14-20 19221709-7 2009 Activation of GPR-54 by kisspeptin also caused reversible increases in [Ca(2+)](i) in Fura-2 loaded dispersed islet cells. Fura-2 86-92 KiSS-1 metastasis-suppressor Mus musculus 24-34 19028081-3 2009 Intracellular fura-2 signals in the hippocampal CA3, in which the basal fura-2 signals were higher in zinc deficiency, were significantly more increased 4s after delivery of kainate (1 mM/1 microl, 1 s) to the dentate granule cell layer. Fura-2 14-20 carbonic anhydrase 3 Rattus norvegicus 48-51 19373968-5 2009 Ratiometric imaging studies with calcium-sensitive dye fura-2 showed that C-INSL7 (10-1000 nM) produced a dose-dependent increase in cytosolic calcium concentrations [Ca2+]i in cultured hypothalamic neurons with two distinct patterns: (1) a sustained elevation lasting for minutes; and (2) a fast, transitory rise followed by oscillations. Fura-2 55-61 relaxin 3 Rattus norvegicus 76-81 19028081-3 2009 Intracellular fura-2 signals in the hippocampal CA3, in which the basal fura-2 signals were higher in zinc deficiency, were significantly more increased 4s after delivery of kainate (1 mM/1 microl, 1 s) to the dentate granule cell layer. Fura-2 72-78 carbonic anhydrase 3 Rattus norvegicus 48-51 18204473-4 2008 EXPERIMENTAL APPROACH: Human TRPC5 channels were expressed in HEK 293 cells and studied using fura-2 and patch-clamp recording to measure intracellular calcium and membrane currents respectively at room temperature. Fura-2 94-100 transient receptor potential cation channel subfamily C member 5 Homo sapiens 29-34 18789885-6 2008 We evaluated the interaction between apoB and cellular membrane by monitoring the change in intracellular Ca(2+) concentration using Fura-2, and found that lipid-free apoB rapidly disrupted the cellular membrane in the absence or presence of the inhibitors for cellular binding/uptake mediated by the receptors. Fura-2 133-139 apolipoprotein B Homo sapiens 37-41 18789885-6 2008 We evaluated the interaction between apoB and cellular membrane by monitoring the change in intracellular Ca(2+) concentration using Fura-2, and found that lipid-free apoB rapidly disrupted the cellular membrane in the absence or presence of the inhibitors for cellular binding/uptake mediated by the receptors. Fura-2 133-139 apolipoprotein B Homo sapiens 167-171 18478545-5 2008 In parallel, Fura-2 imaging of intracellular Ca(2+) concentration ([Ca(2+)](i)) showed that thrombin induced heterogeneous Ca(2+) responses with asynchronous repetitive peaks. Fura-2 13-19 coagulation factor II, thrombin Homo sapiens 92-100 18224450-8 2008 Speculatively, basing our assumptions on Fura2 experiments, we submit the hypothesis that this effect may be due to altered calcium dynamics, which in turn alter cytoskeleton dynamics: the asters, in fact, appear strongly positive to the OTX2 immunoreaction, in both control and exposed samples. Fura-2 41-46 orthodenticle homeobox 2 Homo sapiens 238-242 18400845-6 2008 Using fura-2 loaded cells, we observed that knocking down the expression of NSP4 totally prevented the increase in Ca(2+) permeability of the plasma membrane and cytosolic Ca(2+) concentration measured in infected cells. Fura-2 6-12 serine protease 57 Homo sapiens 76-80 18293404-4 2008 These mechanisms were studied in astrocytes in intact optic nerves isolated from transgenic mice expressing enhanced green fluorescent protein (EGFP) under control of the human glial fibrillary acidic protein promoter (GFAP) by Fura-2 ratiometric Ca2+ imaging. Fura-2 228-234 glial fibrillary acidic protein Homo sapiens 219-223 18056767-9 2008 In experiments performed in fura-2-loaded myocytes exposed to low Na(+) buffer adjusted to pH 6.4, the lower Ca(2+) uptake indicated an inhibitory effect of acidosis on NCX. Fura-2 28-34 solute carrier family 8 member A1 Rattus norvegicus 169-172 18289524-4 2008 Measurement of the Ca2+ signal in fura-2-loaded cells allowed to characterize endothelin-1-evoked Ca2+ entry, which was resistant to dihydropyridine, and was blocked by 2-aminoethoxydiphenylborate (2-APB) and micromolar concentration of Gd3+. Fura-2 34-40 endothelin 1 Homo sapiens 78-90 18568944-5 2008 P2Y(2) receptor desensitization was monitored by following changes in UTP-stimulated intracellular free Ca(2 +) in single cells using fura-2 microfluorometry. Fura-2 134-140 purinergic receptor P2Y2 Homo sapiens 0-6 17868932-5 2008 By fura-2 microfluorometry, NPW (1-100 nM) concentration-dependently increased cytosolic Ca(2+) concentration ([Ca(2+)](i)) at 8.3 mM glucose in rat single beta-cells. Fura-2 3-9 neuropeptide W Rattus norvegicus 28-31 17928534-3 2007 Imaging experiments performed in MIN6 insulinoma cells expressing a genetically encoded cAMP biosensor and loaded with fura-2, a calcium indicator, showed that cAMP oscillations are differentially regulated by periodic changes in membrane potential and GLP-1. Fura-2 119-125 glucagon Mus musculus 253-258 17699555-9 2007 Fura-2 fluorescence measurements revealed that the ANG II-induced Ca(2+) influxes were markedly inhibited in MCs with TRPC6 knockdown, reminiscent of the impaired Ca(2+) entry in response to ANG II in high glucose-treated MCs. Fura-2 0-6 angiotensinogen Rattus norvegicus 51-57 17675532-2 2007 We found that AGP elicited a minimal rise in [Ca2+]i in Fura-2-loaded neutrophils, and this response was markedly enhanced by pretreatment with anti-L-selectin antibodies. Fura-2 56-62 selectin L Homo sapiens 149-159 17959022-4 2007 For the mechanism studies, the intracellular free calcium concentration ([Ca2+]i) and the transcription of caspases-3 and -12 in neurons were detected by Fura 2/AM loading and real-time RT-PCR, respectively. Fura-2 154-160 caspase 3 Rattus norvegicus 107-125 17687000-8 2007 Treatment of ALA increased the intracellular Ca(2+) concentration measured by fura-2 fluorescent microscopy (P < 0.05), showing that ALA may activate AMPK through enhancing Ca(2+)/calmodulin-dependent protein kinase kinase (CaMKK) signaling. Fura-2 78-84 protein kinase AMP-activated catalytic subunit alpha 1 Homo sapiens 153-157 17704188-6 2007 In fura 2-loaded ASM cells, [Ca(2+)](i) responses to 1 microM ACh, 10 microM histamine, and 10 nM bradykinin, as well as SOCE, were attenuated (each to a different extent) after disruption of caveolae by the cholesterol-chelating drug methyl-beta-cyclodextrin. Fura-2 3-9 kininogen 1 Homo sapiens 98-108 17699555-9 2007 Fura-2 fluorescence measurements revealed that the ANG II-induced Ca(2+) influxes were markedly inhibited in MCs with TRPC6 knockdown, reminiscent of the impaired Ca(2+) entry in response to ANG II in high glucose-treated MCs. Fura-2 0-6 transient receptor potential cation channel, subfamily C, member 6 Rattus norvegicus 118-123 17612974-3 2007 Fura-2 microfluorophotometry indicated that endothelin-1 and endothelin-B receptor selective agonists, endothelin-3 and BQ3020, increased the intracellular calcium concentration of the tissue. Fura-2 0-6 endothelin-1 Oryctolagus cuniculus 44-56 17670890-10 2007 In the presence of extracellular Ba(2+), OAG-induced elevations of cytosolic Ba(2+) (fura-2 340-to-380-nm ratio) were significantly larger in astrocytes treated with IL-1beta. Fura-2 85-91 interleukin 1 beta Mus musculus 166-174 17316820-4 2007 In myoglobin-containing mouse heart endogenous chromophores interfere with Fura-2 fluorometry. Fura-2 75-81 myoglobin Mus musculus 3-12 17612974-3 2007 Fura-2 microfluorophotometry indicated that endothelin-1 and endothelin-B receptor selective agonists, endothelin-3 and BQ3020, increased the intracellular calcium concentration of the tissue. Fura-2 0-6 endothelin receptor type B Oryctolagus cuniculus 61-82 17612974-3 2007 Fura-2 microfluorophotometry indicated that endothelin-1 and endothelin-B receptor selective agonists, endothelin-3 and BQ3020, increased the intracellular calcium concentration of the tissue. Fura-2 0-6 endothelin-3 Oryctolagus cuniculus 103-115 17374449-5 2007 In this study, Fura-2 was used to determine [Ca(2+)](i) levels in CA1 hippocampal neurons acutely isolated from young (4-5 months) and mid-age (12-16 months) Sprague-Dawley rats. Fura-2 15-21 carbonic anhydrase 1 Rattus norvegicus 66-69 17465030-6 2007 The biological activity of SMAP8 in rat central neurons was assessed by the calcium microfluorimetric Fura-2 method. Fura-2 102-108 NDRG family member 4 Rattus norvegicus 27-32 17125880-6 2007 Also, the temporal pattern of vasopressin-elicited intracellular calcium oscillations studied on fura2-loaded cells was altered, with the duration of each Ca2+ peak being longer. Fura-2 97-102 arginine vasopressin Rattus norvegicus 30-41 17264311-6 2007 P2X(7) was barely expressed in HMC and showed a relatively scarce functional activity, as assessed by monitoring nucleotide-induced intracellular calcium surge and plasma membrane depolarization by Fura-2/AM and bis[1,3-diethylthiobarbiturate]trimethineoxonal uptake, respectively. Fura-2 198-204 purinergic receptor P2X 7 Homo sapiens 0-6 17317768-7 2007 Low extracellular sodium ([Na(+)](o)) solution increases the intracellular Ca(2+) concentration via NCX transporter in fura-2-loaded osteoclasts. Fura-2 119-125 solute carrier family 8 member A1 Rattus norvegicus 100-103 17389736-11 2007 Fura-2 fluorescence-indicated that Ca(2+) entry in response to AngII stimulation was also dramatically inhibited by TRPC1 antibody and TRPC1-specific RNA interference. Fura-2 0-6 angiotensinogen Rattus norvegicus 63-68 17389736-11 2007 Fura-2 fluorescence-indicated that Ca(2+) entry in response to AngII stimulation was also dramatically inhibited by TRPC1 antibody and TRPC1-specific RNA interference. Fura-2 0-6 transient receptor potential cation channel, subfamily C, member 1 Rattus norvegicus 116-121 17389736-11 2007 Fura-2 fluorescence-indicated that Ca(2+) entry in response to AngII stimulation was also dramatically inhibited by TRPC1 antibody and TRPC1-specific RNA interference. Fura-2 0-6 transient receptor potential cation channel, subfamily C, member 1 Rattus norvegicus 135-140 17286960-4 2007 By Northern blotting, cDNA cloning, and fura-2 monitoring, the branchial ETR subtype was shown to be ET(A) able to induce a Ca(2+) transit. Fura-2 40-46 endothelin receptor type A Homo sapiens 101-106 17446490-3 2007 Here we report that the three isoforms show similar activity evaluated as [Ca2+]i increase evoked by Na+-free medium exposure in Fura-2-loaded single cells and NCX3 transfected cells are less vulnerable to chemical hypoxia compared to NCX1- and NCX2-transfected cells, suggesting that NCX3 could play a more relevant protective role during chemical hypoxia. Fura-2 129-135 solute carrier family 8 member A1 Homo sapiens 235-239 17446490-3 2007 Here we report that the three isoforms show similar activity evaluated as [Ca2+]i increase evoked by Na+-free medium exposure in Fura-2-loaded single cells and NCX3 transfected cells are less vulnerable to chemical hypoxia compared to NCX1- and NCX2-transfected cells, suggesting that NCX3 could play a more relevant protective role during chemical hypoxia. Fura-2 129-135 solute carrier family 8 member A2 Homo sapiens 245-249 17446490-3 2007 Here we report that the three isoforms show similar activity evaluated as [Ca2+]i increase evoked by Na+-free medium exposure in Fura-2-loaded single cells and NCX3 transfected cells are less vulnerable to chemical hypoxia compared to NCX1- and NCX2-transfected cells, suggesting that NCX3 could play a more relevant protective role during chemical hypoxia. Fura-2 129-135 solute carrier family 8 member A3 Homo sapiens 285-289 17209005-8 2007 At the single cardiomyocyte level, VLCAD(-/-) cardiomyocytes showed significant increase in diastolic indo 1 and fura 2 fluorescence, with increased Ca(2+) transient amplitude. Fura-2 113-119 acyl-Coenzyme A dehydrogenase, very long chain Mus musculus 35-45 17446466-7 2007 Experiments with Fura-2/AM-loaded cells revealed an increased cytosolic Ca2+ concentration in the cell lines with blocked PMCA2 isoform. Fura-2 17-23 ATPase plasma membrane Ca2+ transporting 2 Rattus norvegicus 122-127 17982281-4 2007 METHODS: The effect of an immuno-neutralising ECAD on gross morphology, cytosolic calcium signalling, direct cell-to-cell communication and insulin secretion was assessed by fura-2 microfluorimetry, Lucifer Yellow dye injection and insulin ELISA in an insulin-secreting model system. Fura-2 174-180 cadherin 1 Homo sapiens 46-50 16597917-3 2006 As shown by fura-2 ratio measurements while cells were incubated in a temperature-regulated chamber, significant [Ca(2+)](cyt) elevation was elicited by rapid changes in bath temperature, application of TRPV1 receptor agonists capsaicin and resiniferatoxin, or a cold receptor stimulator, icilin. Fura-2 12-18 transient receptor potential cation channel subfamily V member 1 Homo sapiens 203-208 16985238-2 2006 Here, we describe an optical technique to simultaneously image cAMP and Ca2+ concentration gradients in MIN6 mouse insulinoma cells using Epac1-camps, a Forster (or fluorescence) resonance energy transfer (FRET)-based cAMP biosensor, and Fura-2, a fluorescent indicator of Ca2+. Fura-2 238-244 Rap guanine nucleotide exchange factor (GEF) 3 Mus musculus 138-143 16989814-4 2006 Furthermore, Ca(2+) imaging with Fura-2 showed the magnitude of the store-operated Ca(2+) entry to parallel WFS1 expression levels. Fura-2 33-39 wolframin ER transmembrane glycoprotein Homo sapiens 108-112 16095882-2 2006 Using fura-2-loaded human platelets we have found that depletion of the TBHQ sensitive store reduces thrombin--but not ADP--or vasopressin (AVP)-induced Ca2+ release. Fura-2 6-12 coagulation factor II, thrombin Homo sapiens 101-109 16879472-5 2006 Second, Fura-2 loaded NG-108 cells were incubated with (+)pentazocine, a selective sigma-1 receptor agonist, and propofol and then its fluorescence was observed after stimulation with bradykinin. Fura-2 8-14 sigma non-opioid intracellular receptor 1 Mus musculus 83-99 16601133-5 2006 INSL5 (100 nm) administered to dissociated and cultured mouse hypothalamic neurons elevated cytosolic calcium concentrations [Ca(2+)](i), as assessed by the microfluorimetric fura-2 method. Fura-2 175-181 insulin-like 5 Mus musculus 0-5 16600192-5 2006 Fura-2 assay determined significant increases in intracellular free [Ca2+] following exposure to IMN or IFN-gamma. Fura-2 0-6 interferon gamma Mus musculus 104-113 16554405-4 2006 Digital imaging microscopy of cells coloaded with the cytosolic Ca2+ indicator fura-2 revealed that, in parallel, rhod-2 maximally decreased the cytosolic Ca2+ removal rate by 20%. Fura-2 79-85 carbonic anhydrase 2 Homo sapiens 64-67 16554405-4 2006 Digital imaging microscopy of cells coloaded with the cytosolic Ca2+ indicator fura-2 revealed that, in parallel, rhod-2 maximally decreased the cytosolic Ca2+ removal rate by 20%. Fura-2 79-85 carbonic anhydrase 2 Homo sapiens 155-158 16547971-4 2006 Here, we examined the signal transduction pathway linking activation of chemokine receptor CCR5 to an elevation in [Ca(2+)](i) in cultured microglia by using pharmacological approaches in combination with Fura-2-based digital imaging. Fura-2 205-211 C-C motif chemokine receptor 5 Homo sapiens 91-95 16250893-8 2006 Finally, cells treated with histamine and loaded with fura 2 exhibited an improved capacity in eliminating high cytosolic Ca2+ concentrations, in accordance with an increase in activity of a low-affinity Ca2+-ATPase, like SERCA 3. Fura-2 54-60 ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 3 Homo sapiens 222-229 16236332-3 2006 In this study, we studied whether alpha1-adrenoreceptor (alpha1-AR)-activated Ca2+ entry is coupled to both capacitative and non-capacitative pathways in A10 vascular smooth muscle cells by fura-2 fluorescence probe and conventional whole-cell patch clamp techniques. Fura-2 190-196 immunoglobulin kappa variable 6D-21 (non-functional) Homo sapiens 154-157 16380530-7 2006 Consistent with the mechanical characteristics, myocytes treated with leptin displayed a reduced electrically stimulated rise in intracellular Ca2+ (change in fura-2 fluorescence intensity) associated with a prolonged intracellular Ca2+ decay rate. Fura-2 159-165 leptin Mus musculus 70-76 16247029-3 2005 In parallel measurements with fura-2, CaR activation elicited robust Ca2+ oscillations that increased in frequency in the presence of cAMP, eventually fusing into a sustained plateau. Fura-2 30-36 calcium sensing receptor Homo sapiens 38-41 16109841-10 2005 Furthermore, intracellular calcium measurements with fura-2 and fluo-4 showed that activation of the CaSR by Gd(3+) led to a sustained increase in intracellular Ca(2+) even under conditions of low extracellular divalent cations. Fura-2 53-59 calcium sensing receptor Homo sapiens 101-105 16410671-5 2005 The mechanism of adrenomedullin action was studied by fura 2-based intracellular calcium measurement. Fura-2 54-60 adrenomedullin Homo sapiens 17-31 16409471-11 2006 In FURA 2-loaded platelets, HCy induced a dose-dependent intracellular calcium rise suggesting that the calcium elevation promoted by HCy could participate in the cPLA2 activation, leading to arachidonic acid release and TXB2 formation. Fura-2 3-9 phospholipase A2 group IVA Homo sapiens 163-168 15923209-8 2005 TGF-beta (2.5 ng/ml, 18 h) also increased bradykinin-induced intracellular calcium mobilization in fura-2-loaded ASM cells. Fura-2 99-105 transforming growth factor, beta 1 Mus musculus 0-8 15672411-7 2005 In fura-2 loaded PS1 (a prostate smooth muscle cell line) which express endogenous alpha1A-ARs, alpha-agonists epinephrine (EPI), and phenylephrine (PHE) induced Ca(2+) influx which depended on the extracellular Ca(2+) and PLC activation but was independent of PKC activation. Fura-2 3-9 presenilin 1 Rattus norvegicus 17-20 15922343-11 2005 Intracellular Ca(2+) concentrations were monitored in the dispersed PRL cells with the Ca(2+)-sensitive dye, fura-2. Fura-2 109-115 prolactin Homo sapiens 68-71 16091786-4 2005 Expression of functional PAR2 was determined by RT-PCR and by calcium mobilization in Fura-2/AM-loaded cells. Fura-2 86-92 F2R like trypsin receptor 1 Homo sapiens 25-29 15802497-6 2005 Using the fluorescent Ca(2+) indicator, fura-2, we determined that ACTH evokes transient increases in [Ca(2+)](i) and elevates basal Ca(2+) levels in resting chondrocytes. Fura-2 40-46 proopiomelanocortin Homo sapiens 67-71 15605386-12 2005 According to Fura-2-fluorescence capacitative Ca(2+) entry was lower in sgk1-/- MTF compared to sgk1+/+ MTF. Fura-2 13-19 serum/glucocorticoid regulated kinase 1 Mus musculus 72-76 16173930-4 2005 The effects of econazole on intracellular Ca2+ concentrations ([Ca2+]i) in and the proliferation of human PC3 prostate cancer cells was explored in the present study using fura-2 and tetrazolium as fluorescent dyes. Fura-2 172-178 chromobox 8 Homo sapiens 106-109 15855791-4 2005 The FcepsilonRI-induced cellular activation (i.e. cross-linking of FcepsilonRI) was determined by changes in the intracellular level of Ca2+, which was measured by fluorescence of Fura-2. Fura-2 180-186 Fc epsilon receptor Ia Homo sapiens 4-15 15921770-5 2005 VIP enhanced intracellular Ca(2+) levels as evaluated using the calcium probe fura-2. Fura-2 78-84 vasoactive intestinal peptide Homo sapiens 0-3 15916674-5 2005 The response to TRH was studied using the perforated patch technique and Ca(2+) microfluoromety (fura-2). Fura-2 97-103 thyrotropin releasing hormone Rattus norvegicus 16-19 15855791-4 2005 The FcepsilonRI-induced cellular activation (i.e. cross-linking of FcepsilonRI) was determined by changes in the intracellular level of Ca2+, which was measured by fluorescence of Fura-2. Fura-2 180-186 Fc epsilon receptor Ia Homo sapiens 67-78 15913603-4 2005 The contribution of the endogenously expressed TRPV1 channel to intracellular calcium concentration increase in the prostate cells was studied by measuring changes in Fura-2 fluorescence by fluorescence microscopy. Fura-2 167-173 transient receptor potential cation channel subfamily V member 1 Homo sapiens 47-52 15722382-4 2005 METHODS: [Ca(2+)](i) was measured in Fura-2-loaded HEK293(hTRPV1) and HEK293(rTRPV1) cells. Fura-2 37-43 transient receptor potential cation channel subfamily V member 1 Homo sapiens 58-64 15862567-4 2005 Using Ca(i)2+ imaging and the calcium-sensitive dye fura-2, we found that chicken GnRH-II (cGnRH-II) induced a rapid dose-dependent increase in Ca(i)2+ in dispersed tilapia lactotrophs. Fura-2 52-58 mitochondrial ribosomal protein S26 Gallus gallus 82-89 15820503-2 2005 Here, using rat TRPV1 heterologously expressed in Chinese hamster ovary cells, we show that different agonists show different patterns of modulation of the intracellular Ca2+ concentration, monitored in individual cells by fura-2 Ca2+ imaging. Fura-2 223-229 transient receptor potential cation channel, subfamily V, member 1 Rattus norvegicus 16-21 15882549-5 2005 Thrombin-stimulated [Ca2+]i in Ca2+-free and in Ca2+-containing buffers was measured in platelets using the Fura-2 method. Fura-2 108-114 coagulation factor II Rattus norvegicus 0-8 15572518-5 2005 The effect of Src kinase inhibition on ANG II-induced intracellular free Ca(2+) concentration ([Ca(2+)](i)) responses was probed in fura 2-loaded preglomerular microvascular smooth muscle cells (PVSMCs) obtained from explants and studied after 3-5 days in culture. Fura-2 132-138 angiotensinogen Rattus norvegicus 39-45 15731184-7 2005 Antisense knockdown of the TRPC1 gene, proposed to encode endogenous SOCs, markedly reduces SOCE measured with Fura-2. Fura-2 111-117 transient receptor potential cation channel, subfamily C, member 1 Mus musculus 27-32 16121035-4 2005 Fura-2 recordings showed that BDNF acutely elicited an increase in intracellular calcium concentration ([Ca2+]i). Fura-2 0-6 brain derived neurotrophic factor Homo sapiens 30-34 15634940-2 2005 Because of the known signaling pathways for IL-4 and importance of calcium uptake in maintaining SR calcium stores shared by agonists and caffeine, it was hypothesized that this rapid inhibitory effect might depend on phosphatidylinositol 3-kinase (PI3K) and on inhibition of calcium uptake by the SR. Enzyme-dispersed bovine trachealis cells were loaded with Fura-2/acetoxymethyl ester, and changes in cytosolic calcium were imaged in single cells. Fura-2 360-366 phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha Bos taurus 218-247 15716032-5 2005 On the other hand, the basal calcium concentrations in the stratum lucidum and CA3 pyramidal cell layer detected with fura-2 were higher in zinc-deficient mice than in the control mice, indicating that hippocampal calcium homeostasis is affected by zinc deficiency. Fura-2 118-124 carbonic anhydrase 3 Mus musculus 79-82 15644873-3 2005 In fura-2-loaded neurons, nAChR activation evoked a transient increase in [Ca(2+)](I), which was inhibited reversibly and selectively by clinically relevant concentrations of thiopental. Fura-2 3-9 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 26-31 15644873-5 2005 In fura-2-loaded neurons, voltage clamped at -60 mV to eliminate any contribution of voltage-gated Ca(2+) channels, thiopental (25 microM) simultaneously inhibited nAChR-induced increases in [Ca(2+)](i) and peak current amplitudes. Fura-2 3-9 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 164-169 15590764-3 2005 The ability of lymphocyte cholinesterase to inhibit the acetylcholine (ACh)-evoked rise in [Ca(2+)](i) in mouse submandibular acinar cells was determined using fura-2 microfluorimetry. Fura-2 160-166 butyrylcholinesterase Homo sapiens 26-40 15731600-4 2005 [Ca2+]i in CA1 neurons was measured with fura-2 and fura-2 FF. Fura-2 41-47 carbonic anhydrase 2 Rattus norvegicus 1-4 15731600-4 2005 [Ca2+]i in CA1 neurons was measured with fura-2 and fura-2 FF. Fura-2 41-47 carbonic anhydrase 1 Rattus norvegicus 11-14 15731600-4 2005 [Ca2+]i in CA1 neurons was measured with fura-2 and fura-2 FF. Fura-2 52-58 carbonic anhydrase 2 Rattus norvegicus 1-4 15731600-4 2005 [Ca2+]i in CA1 neurons was measured with fura-2 and fura-2 FF. Fura-2 52-58 carbonic anhydrase 1 Rattus norvegicus 11-14 15639476-4 2005 Ang II-induced calcium influx was studied in fura-2-loaded single vascular smooth muscle cells isolated from thoracic aorta of control and diabetic rats. Fura-2 45-51 angiotensinogen Rattus norvegicus 0-6 15644863-7 2005 In the stromal compartment of human prostate tissue, immunoreaction with antibodies against the AT(1) receptor was detectable.Fura-2-loaded hPCPs cells showed an instantaneous and linear rise in free intracellular calcium ion concentration ([Ca(2+)](i)) after local perfusion with Ang II in concentrations of 10 nM. Fura-2 126-132 angiotensinogen Homo sapiens 281-287 16121035-4 2005 Fura-2 recordings showed that BDNF acutely elicited an increase in intracellular calcium concentration ([Ca2+]i). Fura-2 0-6 carbonic anhydrase 2 Homo sapiens 105-108 15203106-5 2004 Spectrofluorimetric measurements in Fura-2 loaded cells showed reduced CCE and Mn2+ entry in response to either thapsigargin or 1alpha,25(OH)2D3 upon transfection with anti-TRPC3/6/7 antisense oligodeoxynucleotides (ODNs). Fura-2 36-42 transient receptor potential cation channel subfamily C member 3 Homo sapiens 173-178 15548706-3 2004 We report evidence from immunofluorescence experiments that in the androgen-responsive LNCaP cell line, the TRPM8 protein is expressed in the endoplasmic reticulum and plasma membrane, acts as a Ca(2+)-permeable channel (assessed using Fura-2 to measure increases in the cytoplasmic Ca(2+) concentration) in each of these membranes, and is regulated by androgen. Fura-2 236-242 transient receptor potential cation channel subfamily M member 8 Homo sapiens 108-113 15649023-0 2004 Problems caused by high concentration of ATP on activation of the P2X7 receptor in bone marrow cells loaded with the Ca2+ fluorophore fura-2. Fura-2 134-140 purinergic receptor P2X, ligand-gated ion channel, 7 Mus musculus 66-79 15372487-6 2004 Alterations of the electrophysiological properties of the Cx45(-/-) cardiac myocytes were indicated both by extracellular recording on planar multielectrode array probes and by intracellular Ca(2+) recording of the fluorescent Ca(2+) indicator fura-2. Fura-2 244-250 gap junction protein, gamma 1 Mus musculus 58-62 15044151-2 2004 Because the canonical transient receptor potential (TRPC) family of proteins was previously shown to comprise Ca(2+)-selective and -nonselective cation channels in a variety of cells, we screened TRPC1-TRPC7 with the use of molecular methods and the fura 2 method to determine their participation as components of the mesangial store-operated Ca(2+) (SOC) channel. Fura-2 250-256 transient receptor potential cation channel, subfamily C, member 1 Mus musculus 196-201 15326217-4 2004 We measured cytosolic Ca(2+) concentration ([Ca(2+)](i)) in fura-2-loaded VSM of anterior mesenteric artery of Squalus acanthias and show that the ET(B)R agonists IRL 1620 and sarafotoxin S6c (SRX) increase [Ca(2+)](i) in VSM to the same extent as ET-1 and ET(B)R appears to be the only ETR subtype in sharks. Fura-2 60-66 endothelin receptor type B Homo sapiens 147-153 15326217-4 2004 We measured cytosolic Ca(2+) concentration ([Ca(2+)](i)) in fura-2-loaded VSM of anterior mesenteric artery of Squalus acanthias and show that the ET(B)R agonists IRL 1620 and sarafotoxin S6c (SRX) increase [Ca(2+)](i) in VSM to the same extent as ET-1 and ET(B)R appears to be the only ETR subtype in sharks. Fura-2 60-66 endothelin 1 Homo sapiens 248-252 15326217-4 2004 We measured cytosolic Ca(2+) concentration ([Ca(2+)](i)) in fura-2-loaded VSM of anterior mesenteric artery of Squalus acanthias and show that the ET(B)R agonists IRL 1620 and sarafotoxin S6c (SRX) increase [Ca(2+)](i) in VSM to the same extent as ET-1 and ET(B)R appears to be the only ETR subtype in sharks. Fura-2 60-66 endothelin receptor type B Homo sapiens 257-263 15362854-5 2004 First, Fura-2 fluorometric Ca(2+) analysis shows the ability of PIP(3) to selectively stimulate [Ca(2+)](i) increase in TRPC6-expressing cells. Fura-2 7-13 prolactin induced protein Homo sapiens 64-67 15362854-5 2004 First, Fura-2 fluorometric Ca(2+) analysis shows the ability of PIP(3) to selectively stimulate [Ca(2+)](i) increase in TRPC6-expressing cells. Fura-2 7-13 transient receptor potential cation channel subfamily C member 6 Homo sapiens 120-125 15309721-8 2004 Fura-2/AM loaded HSC was suspended in no Ca2+ buffer containing 1 mol/L EGTA, 5 min later, 10(-8) mol/L of ET-1 was added, [Ca2+]i inward HSCs rose from resting state to peak 399+/-123 mol/L, then began to come down by the time of 20 min. Fura-2 0-6 endothelin 1 Rattus norvegicus 107-111 15044151-2 2004 Because the canonical transient receptor potential (TRPC) family of proteins was previously shown to comprise Ca(2+)-selective and -nonselective cation channels in a variety of cells, we screened TRPC1-TRPC7 with the use of molecular methods and the fura 2 method to determine their participation as components of the mesangial store-operated Ca(2+) (SOC) channel. Fura-2 250-256 transient receptor potential cation channel, subfamily C, member 7 Mus musculus 202-207 15183114-3 2004 Fura-2 fluorometry revealed that the preceding stimulation with trypsin abolished the subsequent [Ca(2+)](i) response to thrombin, while the responses to PAR1-activating peptides remained intact. Fura-2 0-6 coagulation factor II, thrombin Homo sapiens 121-129 15245434-5 2004 Application of 100 nM HLE resulted in a transient calcium influx in FURA2-loaded human HaCaT keratinocytes observed by single-cell fluorescence imaging. Fura-2 68-73 elastase, neutrophil expressed Homo sapiens 22-25 15481790-5 2004 Intracellular calcium, [Ca2+]i measured by changes in fura-2 level in response to ATP or bradykinin, was significantly inhibited in IgA-IC treated mesangial cells, compared to control cells. Fura-2 54-60 kininogen 1 Homo sapiens 89-99 14978011-8 2004 Simultaneous measures of contractile tension and intracellular free Ca(2+) (fura 2) levels in arterial rings revealed that significantly more tension was produced per unit change in fura 2 ratio in LCX compared with LAD in response to KCl but not during receptor-agonist stimulation with endothelin-1. Fura-2 182-188 endothelin-1 Sus scrofa 288-300 15016832-3 2004 Thrombin or thapsigargin added to the external medium activated Ca2+ entry after Ca2+ store depletion, which we monitored by changes in cellular Fura 2 fluorescence. Fura-2 145-151 coagulation factor II, thrombin Homo sapiens 0-8 15066999-3 2004 STC-1 cells loaded with fura-2 were briefly (3 min) exposed to saturated FA above and below the threshold length (C(8), C(10), and C(12)). Fura-2 24-30 stanniocalcin 1 Mus musculus 0-5 15197535-2 2004 Insulin secretion was determined by a radioimmunoassay (RIA), cytosolic free Ca2+ concentration ([Ca2+]c) with the fluorescence dye fura-2 and whole-cell membrane currents with the patch-clamp technique. Fura-2 132-138 insulin Homo sapiens 0-7 15048927-3 2004 The expression of CXCR4 receptors was demonstrated further in E17 neurospheres using immunohistochemistry, in situ hybridization, Northern blot analysis and fura-2-based Ca(2+) imaging. Fura-2 157-163 chemokine (C-X-C motif) receptor 4 Mus musculus 18-23 14966299-3 2004 Peak-systolic intracellular [Ca(2+)] (measured with Fura-2) was higher in the Ad-FKBP12.6 group compared to Ad-LacZ (1 Hz field stimulation at 37 degrees C). Fura-2 52-58 peptidyl-prolyl cis-trans isomerase FKBP1B Oryctolagus cuniculus 78-89 14966371-3 2004 Among a variety of Ca(2+)-related agents tested, EGTA and thapsigargin were found to suppress both the glucose uptake and intracellular Ca(2+) mobilization induced by ET-1, as determined by Fura-2 analysis. Fura-2 190-196 endothelin 1 Homo sapiens 167-171 14724272-10 2004 Fura-2 microfluorimetry revealed that vitronectin induced a significant and sustained increase in intracellular Ca2+ concentration, although vitronectin-induced Ca2+ current could not be detected. Fura-2 0-6 vitronectin Bos taurus 38-49 12944324-3 2004 Digital fluorescence microscopy and fura 2 were used to monitor intracellular Ca2+ concentration ([Ca2+]i) changes in response to thrombin and PAR1-activating peptide (PAR1-AP) SFFLRN. Fura-2 36-42 coagulation factor II, thrombin Homo sapiens 130-138 12944324-3 2004 Digital fluorescence microscopy and fura 2 were used to monitor intracellular Ca2+ concentration ([Ca2+]i) changes in response to thrombin and PAR1-activating peptide (PAR1-AP) SFFLRN. Fura-2 36-42 coagulation factor II thrombin receptor Homo sapiens 143-147 12944324-3 2004 Digital fluorescence microscopy and fura 2 were used to monitor intracellular Ca2+ concentration ([Ca2+]i) changes in response to thrombin and PAR1-activating peptide (PAR1-AP) SFFLRN. Fura-2 36-42 coagulation factor II thrombin receptor Homo sapiens 168-172 14733770-8 2004 The cytosolic free calcium concentration induced by UII was measured using Fura-2/AM. Fura-2 75-81 urotensin 2 Homo sapiens 52-55 12960768-1 2003 A 1-2 min application of brain-derived neurotrophic factor (BDNF; 20 ng/ml) induced sustained elevation of intracellular Ca2+ lasting > 90 min, using the fura-2 imaging of intracellular Ca2+ mobilization, in visual cortical pyramidal neurons isolated from rats. Fura-2 157-163 brain-derived neurotrophic factor Rattus norvegicus 25-58 14727511-6 2003 Moreover, fura-2-imaging analyses in midbrain slices demonstrated that nicotine-elicited Ca2+ mobilization was diminished in beta 2-/- mice. Fura-2 10-16 hemoglobin, beta adult minor chain Mus musculus 125-131 12829428-6 2003 In this study, the effects of ANG II and KCl on fura 2-measured cytosolic Ca2+ concentration ([Ca2+]i) responses in cultured area postrema neurons in the presence and absence of 12-h exposure to 100 nM 17 beta-estradiol (E2) were evaluated. Fura-2 48-54 angiotensinogen Homo sapiens 30-36 14638730-9 2003 Intracellular Ca(2+) ([Ca(2+)](i)) mobilization mediated by ET-1 was measured using the Fura-2 AM fluorescent probe technique as an index of ET receptor function. Fura-2 88-94 endothelin 1 Homo sapiens 60-64 12908873-9 2003 In the present study, we have used quenched- and stopped-flow approaches to determine the latencies of thrombin-evoked coupling of Ins P3RII with hTRPC1 and of thrombin-evoked bivalent cation entry using Mn2+ quenching of fura 2 fluorescence. Fura-2 222-228 coagulation factor II, thrombin Homo sapiens 103-111 12908873-9 2003 In the present study, we have used quenched- and stopped-flow approaches to determine the latencies of thrombin-evoked coupling of Ins P3RII with hTRPC1 and of thrombin-evoked bivalent cation entry using Mn2+ quenching of fura 2 fluorescence. Fura-2 222-228 coagulation factor II, thrombin Homo sapiens 160-168 12960768-1 2003 A 1-2 min application of brain-derived neurotrophic factor (BDNF; 20 ng/ml) induced sustained elevation of intracellular Ca2+ lasting > 90 min, using the fura-2 imaging of intracellular Ca2+ mobilization, in visual cortical pyramidal neurons isolated from rats. Fura-2 157-163 brain-derived neurotrophic factor Rattus norvegicus 60-64 12595579-5 2003 A deficit in DMPK caused enhanced basal contractility of single cardiomyocytes and an associated increase in intracellular Ca(2+), measured using fura-2. Fura-2 146-152 dystrophia myotonica-protein kinase Mus musculus 13-17 12893253-4 2003 The function of the novel murine TRPM4 was studied in HEK-293 cells using a fluorescent calcium indicator, fura-2. Fura-2 107-113 transient receptor potential cation channel, subfamily M, member 4 Mus musculus 33-38 12805242-5 2003 In voltage-clamped, fura 2-loaded myocytes (20 degrees C), a reduced peak-systolic and end-diastolic [Ca2+]i was observed after Ad-sorcin transfection. Fura-2 20-26 sorcin Oryctolagus cuniculus 131-137 12767893-0 2003 Cytoplasmic calcium measurement in rotavirus enterotoxin-enhanced green fluorescent protein (NSP4-EGFP) expressing cells loaded with Fura-2. Fura-2 133-139 serine protease 57 Homo sapiens 93-97 12815712-3 2003 Using computer-assisted imaging of fura-2 fluorescence, we found an elevation of intracellular cytoplasmic calcium in cortical astrocytes from neonatal Ts65Dn mouse brain, similar to that observed previously in embryonic Ts16 astrocytes. Fura-2 35-41 reciprocal translocation, Chr 16, cytogenetic band C3-4; and Chr 17, cytogenetic band A2, Davisson 65 Mus musculus 152-158 12494452-2 2003 In this study, the changes in [Ca(2+)](i) elicited by epidermal growth factor (EGF) in rat cardiac microvascular endothelial cells (CMEC) have been investigated by using fura-2 conventional and confocal microscopy. Fura-2 170-176 epidermal growth factor like 1 Rattus norvegicus 54-77 12614345-7 2003 The mGluR5 receptor function was assessed in CG-4 OPCs with fura-2 microfluorometry. Fura-2 60-66 glutamate receptor, ionotropic, kainate 1 Mus musculus 4-10 12494452-2 2003 In this study, the changes in [Ca(2+)](i) elicited by epidermal growth factor (EGF) in rat cardiac microvascular endothelial cells (CMEC) have been investigated by using fura-2 conventional and confocal microscopy. Fura-2 170-176 epidermal growth factor like 1 Rattus norvegicus 79-82 12388389-5 2003 Also, application of ANG II significantly increased intracellular Ca(2+) concentrations, which were measured with fura 2 dye. Fura-2 114-120 angiotensinogen Rattus norvegicus 21-27 12479978-5 2003 Angiotensin II evoked an increase in intracellular Ca(2+) in fura-2-loaded human gingival fibroblasts. Fura-2 61-67 angiotensinogen Homo sapiens 0-14 12522081-1 2003 1 Using fura-2 fluorometry of [Ca(2+)](i) in response to thrombin, trypsin and protease-activated receptor activating peptides (PAR-APs), we determined whether trypsin cleaves protease-activated receptor 1 (PAR1) and activates it in the endothelial cells of the porcine aortic valves and human umbilical vein. Fura-2 8-14 coagulation factor II, thrombin Homo sapiens 57-65 12540526-13 2003 Addition of the bradykinin B(1) receptor agonist [des-Arg(9)]-bradykinin (100 nM) to Fura-2-loaded peritubular cells did not change the [Ca(2+)](i). Fura-2 85-91 bradykinin receptor B1 Rattus norvegicus 16-40 12522076-1 2003 1 In fura 2-loaded HEK-293 cells stably expressing human type 1 parathyroid hormone (PTH) receptors, PTH potentiated the Ca(2+) mobilization evoked by carbachol by >4 fold without itself increasing the intracellular [Ca(2+)]. Fura-2 5-11 parathyroid hormone Homo sapiens 64-83 12867160-1 2003 Bradykinin (1 microM) and histamine (100 microM) evoked an initial transient increase and a subsequent sustained increase in intracellular Ca(2+) concentration ([Ca(2+)](i)) in fura-2-loaded human gingival fibroblasts, which may be attributed to Ca(2+) release from intracellular stores and Ca(2+) entry from extracellular sites, respectively. Fura-2 177-183 kininogen 1 Homo sapiens 0-10 12522076-1 2003 1 In fura 2-loaded HEK-293 cells stably expressing human type 1 parathyroid hormone (PTH) receptors, PTH potentiated the Ca(2+) mobilization evoked by carbachol by >4 fold without itself increasing the intracellular [Ca(2+)]. Fura-2 5-11 parathyroid hormone Homo sapiens 85-88 12522076-1 2003 1 In fura 2-loaded HEK-293 cells stably expressing human type 1 parathyroid hormone (PTH) receptors, PTH potentiated the Ca(2+) mobilization evoked by carbachol by >4 fold without itself increasing the intracellular [Ca(2+)]. Fura-2 5-11 parathyroid hormone Homo sapiens 101-104 12508053-8 2003 Fura-2 quenching experiments with Mn(2+), Co(2+), or Ni(2+) suggest that these can be transported by TRPM7 in the presence of physiological levels of Ca(2+) and Mg(2+), suggesting that TRPM7 represents a novel ion-channel mechanism for cellular metal ion entry into vertebrate cells. Fura-2 0-6 transient receptor potential cation channel subfamily M member 7 Homo sapiens 101-106 12508053-8 2003 Fura-2 quenching experiments with Mn(2+), Co(2+), or Ni(2+) suggest that these can be transported by TRPM7 in the presence of physiological levels of Ca(2+) and Mg(2+), suggesting that TRPM7 represents a novel ion-channel mechanism for cellular metal ion entry into vertebrate cells. Fura-2 0-6 transient receptor potential cation channel subfamily M member 7 Homo sapiens 185-190 12388458-1 2002 Imaging fluorescent measurements with fura 2 were used to examine cytosolic calcium signals induced by sulfated CCK octapeptide (CCK-8) in dissociated vagal afferent neurons from adult rat nodose ganglia. Fura-2 38-44 cholecystokinin Rattus norvegicus 112-115 12509487-1 2003 Functional coupling between Ca(2+)-induced Ca(2+) release (CICR) and quantal exocytosis in 5-hydroxytryptamine-loaded INS-1 beta cells was assessed through the use of carbon fibre amperometry in combination with Fura-2. Fura-2 212-218 insulin 1 Rattus norvegicus 118-123 12379639-5 2002 The NCKX4 protein demonstrated potassium-dependent sodium calcium exchanger activity when assayed in transfected HEK293 cells using digital imaging of fura-2 fluorescence. Fura-2 151-157 solute carrier family 24 member 4 Homo sapiens 4-9 12388458-1 2002 Imaging fluorescent measurements with fura 2 were used to examine cytosolic calcium signals induced by sulfated CCK octapeptide (CCK-8) in dissociated vagal afferent neurons from adult rat nodose ganglia. Fura-2 38-44 cholecystokinin Rattus norvegicus 129-132 12351388-6 2002 Using Fura-2-loaded platelets, we report that, in line with TRPC6 expression, 1-oleoyl-2-acetyl-sn-glycerol (OAG) stimulated the entry of Ca(++) and Ba(2+) independently of protein kinase C. Thrombin also induced the entry of Ca(++) and Ba(2+), but thapsigargin, which depletes the stores, induced the entry of only Ca(++). Fura-2 6-12 transient receptor potential cation channel subfamily C member 6 Homo sapiens 60-65 12427859-10 2002 Additionally, exogenous cholecystokinin was effective in elevating intracellular calcium as measured by ratiometric techniques with the calcium-sensitive dye fura-2. Fura-2 158-164 cholecystokinin Rattus norvegicus 24-39 12372800-3 2002 Fura 2 fluorescence ratiometry showed that the thapsigargin-induced Ca2+ entry (equivalent to SOC) was significantly inhibited by 1 microM Go-6976 (a specific PKCalpha and betaI inhibitor) and PKCalpha antisense treatment (2.5 nM for 24-48 h). Fura-2 0-6 protein kinase C alpha Homo sapiens 159-167 12372800-3 2002 Fura 2 fluorescence ratiometry showed that the thapsigargin-induced Ca2+ entry (equivalent to SOC) was significantly inhibited by 1 microM Go-6976 (a specific PKCalpha and betaI inhibitor) and PKCalpha antisense treatment (2.5 nM for 24-48 h). Fura-2 0-6 protein kinase C alpha Homo sapiens 193-201 12411465-8 2002 Moreover, angiotensin II stimulation of divalent cation influx (Mn2+ quench of fura-2 fluorescence) was decreased significantly by AG1478. Fura-2 79-85 angiotensinogen Rattus norvegicus 10-24 12405954-9 2002 Fura-2 imaging revealed that a brief application of BDNF elicited a long-lasting elevation of intracellular Ca2+. Fura-2 0-6 brain-derived neurotrophic factor Rattus norvegicus 52-56 12217877-4 2002 Using fura 2-loaded preparations, we demonstrated that 10 nM angiotensin II (ANG II) elevates pericyte intracellular Ca(2+) concentration ([Ca(2+)](i)) and suppresses endothelial [Ca(2+)](i). Fura-2 6-12 angiotensinogen Homo sapiens 61-75 12217877-4 2002 Using fura 2-loaded preparations, we demonstrated that 10 nM angiotensin II (ANG II) elevates pericyte intracellular Ca(2+) concentration ([Ca(2+)](i)) and suppresses endothelial [Ca(2+)](i). Fura-2 6-12 angiotensinogen Homo sapiens 77-83 12383867-1 2002 The responsiveness of cultured myenteric neurons to cholecystokinin (CCK-8) was examined using fura-2-based digital microfluorimetric measurement of intracellular calcium ([Ca(2+)](i)). Fura-2 95-101 cholecystokinin Cavia porcellus 69-72 12218532-5 2002 METHODS: The effect of 0.5-3% isoflurane was studied (1) on AngII (10 m)-induced intracellular Ca mobilization, intracellular Ca release from internal stores, and Ca influx in Fura-2 loaded cultured aortic vascular smooth muscle cell isolated from 6-week-old Wistar Kyoto and spontaneously hypertensive rats, using fluorescent imaging microscopy; and (2) on the organization of cytoskeletal elements, using immunofluorescence labeling. Fura-2 176-182 angiotensinogen Rattus norvegicus 60-65 12378642-6 2002 Cholecystokinin (CCK)-induced increases in intracellular calcium in single cells were measured with fura-2 microfluorometry. Fura-2 100-106 cholecystokinin Rattus norvegicus 0-15 12378642-6 2002 Cholecystokinin (CCK)-induced increases in intracellular calcium in single cells were measured with fura-2 microfluorometry. Fura-2 100-106 cholecystokinin Rattus norvegicus 17-20 12213305-3 2002 Ca(2+) stores discharged by application of 100 microM muscarine (in Ca(2+)-free perfusate) in PS1wt and PS1 DeltaE9 cells were significantly larger than those in control cells, as determined using Fura-2 microfluorimetry. Fura-2 197-203 presenilin 1 Homo sapiens 94-97 12167589-7 2002 We first confirmed that Gd(3+) activated the CaSR by measuring intracellular Ca(2+) concentration ([Ca(2+)](i)) in CALs loaded with fura 2. Fura-2 132-138 calcium-sensing receptor Mus musculus 45-49 12167589-7 2002 We first confirmed that Gd(3+) activated the CaSR by measuring intracellular Ca(2+) concentration ([Ca(2+)](i)) in CALs loaded with fura 2. Fura-2 132-138 carbonic anhydrase 8 Mus musculus 115-119 12210833-4 2002 This investigation, employing fura-2 fluorescent measurements and specific channel blockers and other agents, revealed that GM1 modulates a hitherto unidentified Ca(2+) channel not of the L type. Fura-2 30-36 coenzyme Q10A Mus musculus 124-127 12147606-6 2002 ET-1-induced intracellular calcium ([Ca2+]i) in hONAs was measured by fura-2 imaging. Fura-2 70-76 endothelin 1 Homo sapiens 0-4 12065595-5 2002 Imaging of fura-2-loaded acinar cells revealed that the rate of [Ca(2+)](c) rise during CCK-evoked oscillations slows with each subsequent oscillation, consistent with a developing modulation of release, whereas the kinetics of ACh-evoked oscillations remain constant. Fura-2 11-17 cholecystokinin Homo sapiens 88-91 12154174-5 2002 In fura-2-loaded cells extracellular 2-aminoethoxydiphenyl borate and intracellular heparin (5 mg ml(-1)) both inhibited the global cytoplasmic [Ca(2+)] transient evoked by carbachol, confirming that it was IP(3)R-dependent. Fura-2 3-9 inositol 1,4,5-trisphosphate receptor, type 1 Rattus norvegicus 207-213 12088846-5 2002 Analysis of intracellular calcium dynamics using the calcium-sensitive dye fura-2 AM indicated that exposure of cortical astrocytes to V(1) vasopressin receptor agonist, [Phe(2),Orn(8)]-oxytocin, induced a marked dose-dependent increase in intracellular calcium which was abolished by depletion of extracellular calcium. Fura-2 75-81 arginine vasopressin Homo sapiens 140-151 12194015-7 2002 Using fura-2 fluorescence ratiometry to assess [Ca(2+)](i), CCK (<or=10(-10) M)-induced Ca(2+) oscillations were observed in RPAs. Fura-2 6-12 cholecystokinin Rattus norvegicus 60-63 11874470-2 2002 Fluorescence ratio imaging indicates that immobilized, aspirin-treated platelets, loaded with Fura-2, respond to inositol 1,4,5-trisphosphate- (InsP3)-generating agonists such as thrombin by high-frequency, irregular rises in cytosolic [Ca2+]i with spikes that vary in peak level and peak-to-peak interval. Fura-2 94-100 coagulation factor II, thrombin Homo sapiens 179-187 11926949-12 2002 In fura-2-loaded cells from patients with UC, the NKA-induced Ca(2+) signal was also significantly reduced in Ca(2+)-free medium, indicating the reduced intracellular Ca(2+) stores after UC. Fura-2 3-9 tachykinin precursor 1 Homo sapiens 50-53 12091553-4 2002 The ability of extracellularly applied U73122 to inhibit PLC was confirmed by its antagonism of ATP-induced elevation of intracellular Ca(2+) as measured by fura-2 photometry. Fura-2 157-163 heparan sulfate proteoglycan 2 Homo sapiens 57-60 12148843-7 2002 The peak increase of [Ca2+]i induced by cell swelling was 3.40 +/- 0.49 (Fura-2 F340/F380 fluorescence ratio, n = 11) and 3.17 +/- 0.43 (n = 17) in the presence and the absence of extracellular Ca2+, respectively, corresponding to an absolute [Ca2+]i of around 1 microm. Fura-2 73-79 carbonic anhydrase 2 Homo sapiens 22-25 11781140-1 2002 In fura-2 loaded isolated mouse pancreatic acinar cells, xanthine oxidase (XOD)-catalyzed reactive oxygen species (ROS) generation caused an increase in the cytosolic Ca(2+) concentration ([Ca(2+)](i)) by release of Ca(2+) from intracellular stores. Fura-2 3-9 xanthine dehydrogenase Mus musculus 57-73 11889203-6 2002 In fura-2-loaded HEK293 cells expressing the wild-type or mutant receptors, the inactivating R220W mutant produced a significant shift to the right relative to the wild-type CASR in the cytosolic calcium response to increasing extracellular calcium concentrations and the G549R and C850-851 ins/fs mutants were without detectable activity. Fura-2 3-9 calcium sensing receptor Homo sapiens 174-178 11750919-10 2002 However, responses to TNF were mimicked by sphingolipids, including sphingosine-1-phosphate, which evoked a transient rises in [Ca2+]i in a pertussis toxin-insensitive manner in fura-2-loaded DRG neurones. Fura-2 178-184 tumor necrosis factor Rattus norvegicus 22-25 11818209-8 2002 Accumulation and efflux studies with the P-gp substrates, Dox and Fura-2, demonstrated that Tet inhibited the P-gp-mediated drug efflux. Fura-2 66-72 ATP binding cassette subfamily B member 1 Homo sapiens 41-45 11818209-8 2002 Accumulation and efflux studies with the P-gp substrates, Dox and Fura-2, demonstrated that Tet inhibited the P-gp-mediated drug efflux. Fura-2 66-72 ATP binding cassette subfamily B member 1 Homo sapiens 110-114 11773318-8 2002 The I(mtx) activation time course reflected the changes in intracellular Ca(2+) and Na(+) measured with the fluorescent indicators fura-2 and SBFI, respectively, suggesting that the activation of I(mtx) brings about an increment in intracellular Ca(2+) and Na(+). Fura-2 131-137 metaxin 1 Homo sapiens 6-9 11773318-8 2002 The I(mtx) activation time course reflected the changes in intracellular Ca(2+) and Na(+) measured with the fluorescent indicators fura-2 and SBFI, respectively, suggesting that the activation of I(mtx) brings about an increment in intracellular Ca(2+) and Na(+). Fura-2 131-137 metaxin 1 Homo sapiens 198-201 11750919-8 2002 TNF-evoked transient increases in [Ca2+]i were also detected in 34% of fura-2-loaded DRG neurones. Fura-2 71-77 tumor necrosis factor Rattus norvegicus 0-3 11750919-8 2002 TNF-evoked transient increases in [Ca2+]i were also detected in 34% of fura-2-loaded DRG neurones. Fura-2 71-77 carbonic anhydrase 2 Rattus norvegicus 35-38 11750919-10 2002 However, responses to TNF were mimicked by sphingolipids, including sphingosine-1-phosphate, which evoked a transient rises in [Ca2+]i in a pertussis toxin-insensitive manner in fura-2-loaded DRG neurones. Fura-2 178-184 carbonic anhydrase 2 Rattus norvegicus 128-131 11755115-1 2001 In the present study we have employed fura-2 loaded isolated mouse pancreatic acinar cells to monitor the effect that xanthine oxidase (XOD)-catalyzed reactive oxygen species generation presents on Ca(2+) mobilization by the secretagogue cholecystokinin octapeptide (CCK-8). Fura-2 38-44 xanthine dehydrogenase Mus musculus 118-134 11755115-1 2001 In the present study we have employed fura-2 loaded isolated mouse pancreatic acinar cells to monitor the effect that xanthine oxidase (XOD)-catalyzed reactive oxygen species generation presents on Ca(2+) mobilization by the secretagogue cholecystokinin octapeptide (CCK-8). Fura-2 38-44 xanthine dehydrogenase Mus musculus 136-139 11514552-5 2001 Expression of dFKBP59 produced an inhibition of Ca(2+) influx via TRPL in fura-2 assays. Fura-2 74-80 FK506-binding protein 59kD Drosophila melanogaster 14-21 11726808-2 2001 Here we studied ET-1-induced changes in intracellular calcium (Ca2+in) in Fura-2 loaded mouse neuroblastoma-rat dorsal root ganglion hybrid cells (ND7/104). Fura-2 74-80 endothelin 1 Mus musculus 16-20 11514552-5 2001 Expression of dFKBP59 produced an inhibition of Ca(2+) influx via TRPL in fura-2 assays. Fura-2 74-80 transient receptor potential-like Drosophila melanogaster 66-70 11447011-7 2001 Fura 2-loaded SCBN cells had increased fluorescence after PAR-1 activation, indicating increased intracellular Ca(2+). Fura-2 0-6 coagulation factor II thrombin receptor Homo sapiens 58-63 11513973-7 2001 Cells expressing hTRPC-1 exhibited a substantial enhancement of maitotoxin-initiated Ca(2+) inflow and a modest enhancement of thapsigargin-initiated Ca(2+) inflow (measured using fura-2) and no enhancement of the highly Ca(2+)-selective store-operated Ca(2+) current (measured using patch-clamp recording). Fura-2 180-186 transient receptor potential cation channel subfamily C member 1 Homo sapiens 17-24 11487506-5 2001 Thrombin and the PAR1 agonist TFLLR-NH(2) (TF-NH(2)) increased [Ca(2+)](i) >50% of cultured neurons (EC(50)s 24 mu ml(-1) and 1.9 microM, respectively), assessed using Fura-2 AM. Fura-2 171-177 coagulation factor II Rattus norvegicus 0-8 11487506-5 2001 Thrombin and the PAR1 agonist TFLLR-NH(2) (TF-NH(2)) increased [Ca(2+)](i) >50% of cultured neurons (EC(50)s 24 mu ml(-1) and 1.9 microM, respectively), assessed using Fura-2 AM. Fura-2 171-177 coagulation factor II (thrombin) receptor Rattus norvegicus 17-21 11448475-3 2001 In this study, we have measured capsaicin- and anandamide-induced elevations in intracellular calcium concentrations ([Ca(2+)](i)) in fura-2 loaded HEK293 cells stably expressing the rat VR1 receptor at 22, 37 and 50 degrees C. Both capsaicin and anandamide produced a concentration-dependent elevation in [Ca(2+)](i) at all temperatures. Fura-2 134-140 transient receptor potential cation channel, subfamily V, member 1 Rattus norvegicus 187-190 11483701-2 2001 To determine the mechanism of bradykinin-induced production of endothelium-derived contracting factors, we monitored the changes in cytosolic Ca(2+) concentration ([Ca(2+)](i)) in in situ endothelial cells in porcine aortic valvular strips and the changes in [Ca(2+)](i) of smooth muscle cells and force in porcine interlobar renal arterial strips using front-surface fluorometry of fura-2. Fura-2 383-389 kininogen 1 Homo sapiens 30-40 11171105-2 2001 In freshly-isolated rat hepatocytes, 2-APB inhibited thapsigargin- and vasopressin-stimulated Ca2+ inflow (measured using fura-2) with no detectable effect on the release of Ca2+ from intracellular stores. Fura-2 122-128 arginyl aminopeptidase Rattus norvegicus 39-42 11294880-4 2001 As anticipated from this sequence similarity, NCKX3 displayed K(+)-dependent Na(+)/Ca(2+) exchanger activity when assayed in heterologous expression systems, using digital imaging of fura-2 fluorescence, electrophysiology, or radioactive (45)Ca(2+) uptake. Fura-2 183-189 solute carrier family 24 member 3 Homo sapiens 46-51 11373474-6 2001 The effect of anesthetics on NMDA receptors was determined by measuring the NMDA-mediated changes in intracellular calcium in the CA1 pyramidal layer with a calcium indicator, fura-2. Fura-2 176-182 carbonic anhydrase 1 Rattus norvegicus 130-133 11287471-8 2001 Cellular Ca(2+) fluorescence measurement using fura-2 indicated that neurotensin rapidly induced Ca(2+) release from intracellular stores of PAG-RVM neurons. Fura-2 47-53 neurotensin Rattus norvegicus 69-80 11171105-9 2001 In the H4-IIE rat hepatoma cell line, 2-APB inhibited thapsigargin-stimulated Ca2+ inflow (measured using fura-2) and, in whole-cell patch-clamp experiments, the Ins(1,4,5)P(3)-induced inward current carried by Ca2+. Fura-2 106-112 arginyl aminopeptidase Rattus norvegicus 40-43 11240034-6 2001 Direct application of either human IgM anti-GM1 antibodies or the B subunit of CT to N18 neuroblastoma cells induced a sustained influx of manganese ions, as indicated by a quench of the intracellular fura-2 fluorescence. Fura-2 201-207 coenzyme Q10A Mus musculus 44-47 11250874-2 2001 This study investigates the mechanism of CGRP-induced relaxation in intramural coronary arteries by determining the effect of CGRP on cytosolic Ca(2+) concentration ([Ca(2+)](i)) using FURA-2 technique. Fura-2 185-191 calcitonin-related polypeptide alpha Rattus norvegicus 41-45 11281550-3 2001 METHODS AND RESULTS: Stimulation of HSCs with ADM resulted in a dose-dependent raise in cAMP concentration (radioimmunoassay) and markedly blunted the endothelin-induced increase in [Ca2+]i and cell contraction, as assessed in cells loaded with fura-2 using a morphometric method. Fura-2 245-251 adrenomedullin Homo sapiens 46-49 11805429-1 2001 BACKGROUND: The effect of bradykinin on intracellular free Ca(2+) levels ([Ca(2+)](i)) in MG63 human osteosarcoma cells was explored using fura-2 as a Ca(2+) dye. Fura-2 139-145 kininogen 1 Homo sapiens 26-36 11509826-5 2001 Ca(i) of primary cultured rat alveolar type II cells was measured by fura-2 epifluorescence. Fura-2 69-75 carbonic anhydrase 1 Rattus norvegicus 0-5 11136854-1 2001 Patch clamp and fura-2 fluorescence were employed to characterize receptor-mediated activation of recombinant Drosophila TrpL channels expressed in Sf9 insect cells. Fura-2 16-22 transient receptor potential-like Drosophila melanogaster 121-125 11152740-4 2001 Here, we used glutamate iontophoresis to induce Ca(2+) transients in hippocampal CA1 neurons injected with the Ca(2+)-indicator fura-2. Fura-2 128-134 carbonic anhydrase 1 Homo sapiens 81-84 11136854-4 2001 In fura-2 assays, extracellular application of bacterial phosphatidylinositol (PI)-PLC or phosphatidylcholine (PC)-PLC caused a transient increase in TrpL channel activity, the magnitude of which was significantly less than that observed following receptor stimulation. Fura-2 3-9 Phospholipase C at 21C Drosophila melanogaster 83-86 11136854-4 2001 In fura-2 assays, extracellular application of bacterial phosphatidylinositol (PI)-PLC or phosphatidylcholine (PC)-PLC caused a transient increase in TrpL channel activity, the magnitude of which was significantly less than that observed following receptor stimulation. Fura-2 3-9 Phospholipase C at 21C Drosophila melanogaster 115-118 11136854-4 2001 In fura-2 assays, extracellular application of bacterial phosphatidylinositol (PI)-PLC or phosphatidylcholine (PC)-PLC caused a transient increase in TrpL channel activity, the magnitude of which was significantly less than that observed following receptor stimulation. Fura-2 3-9 transient receptor potential-like Drosophila melanogaster 150-154 11139441-1 2000 Using a method employing front-surface fura-2 fluorometry to measure the cytosolic Ca(2+) concentration, [Ca(2+)](i), the mechanism of endothelium-dependent regulation of vascular tone by thrombin was studied in porcine renal interlobar arterial strips. Fura-2 39-45 coagulation factor II, thrombin Homo sapiens 188-196 11135617-5 2001 Moreover, the treatment with recombinant t-PA leads to a 37% increase in NMDA-stimulated fura-2 fluorescence, which may reflect an increased NMDA-receptor function. Fura-2 89-95 plasminogen activator, tissue type Homo sapiens 41-45 11440808-7 2001 Consistent with abrogation of excitotoxic pathways, fura-2 imaging showed selective deficits in gp120-dependent intracellular Ca2+ responses in ethanol-pretreated slices. Fura-2 52-58 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 96-101 11145120-5 2000 The intracellular Ca2+ concentration ([Ca2+]i) increased in fura-2-loaded ZLC islets following stimulation with 12 mmol/L glucose alone or GLP-1 in the presence of 12 mmol/L glucose. Fura-2 60-66 glucagon Rattus norvegicus 139-144 11179848-2 2000 In vitro, we noticed that the NMDA-evoked increase in Fura-2 detected intracellular Ca(2+) concentration in synaptoneurosomes from the rat, but not from the rabbit hippocampus, was strongly inhibited by the ryanodine receptor (RyR) antagonists dantrolene and ryanodine. Fura-2 54-60 LOC100009439 Oryctolagus cuniculus 207-225 11179848-2 2000 In vitro, we noticed that the NMDA-evoked increase in Fura-2 detected intracellular Ca(2+) concentration in synaptoneurosomes from the rat, but not from the rabbit hippocampus, was strongly inhibited by the ryanodine receptor (RyR) antagonists dantrolene and ryanodine. Fura-2 54-60 LOC100009439 Oryctolagus cuniculus 227-230 11032778-2 2000 In this study, spectrofluorimetric experiments in free solution, using Fluo-3 and Fura-2, showed that Zn2+ and Cd2+ enhanced the probe signal, Cu2+ quenched it, and Hg2+ had no effect. Fura-2 82-88 CD2 molecule Homo sapiens 111-114 11063339-13 2000 The AT1R was functional since a significant increase in [Ca(i)] was observed in Fura-2 loaded day 10 cells when stimulated with AII (182%, P<0.0001). Fura-2 80-86 angiotensin II receptor type 1 Homo sapiens 4-8 11063339-13 2000 The AT1R was functional since a significant increase in [Ca(i)] was observed in Fura-2 loaded day 10 cells when stimulated with AII (182%, P<0.0001). Fura-2 80-86 angiotensinogen Homo sapiens 128-131 11025441-5 2000 The function of RyR in Ca(2+) signaling was investigated using single-cell fura-2 imaging. Fura-2 75-81 ryanodine receptor 1 Homo sapiens 16-19 11056101-5 2000 After washout of the alpha-ADR agonist, simultaneous measurement of [Ca(2+)](i) transients with fura 2 and myocyte shortening by video edge-detection showed that [Ca(2+)](i) amplitude and myocyte shortening were decreased in alpha-ADR-treated myocytes, and the time to peak and time from peak to 80% decline of both [Ca(2+)](i) and myocyte shortening were increased. Fura-2 96-102 carbonic anhydrase 2 Rattus norvegicus 69-75 11027555-2 2000 Bradykinin (10 nM) and thapsigargin (1 microM) provoked large Ca(2+) influxes under fura-2/AM fluoroscopy. Fura-2 84-90 kininogen 1 Homo sapiens 0-10 11032778-4 2000 Digital imaging of living mussel haemocytes loaded with Fura-2/AM or Fluo-3/AM showed that Hg2+, Cu2+ and Cd2+ induced a rise in probe fluorescence, whereas up to 200 microM Zn2+ had no effect. Fura-2 56-62 CD2 molecule Homo sapiens 106-109 11009559-1 2000 Angiotensin II (Ang II)-induced Ca(2+) signaling was studied in isolated rat renal arterioles using fura-2. Fura-2 100-106 angiotensinogen Rattus norvegicus 0-22 10928946-4 2000 Measuring [Ca(2+)](i) by imaging analysis of fura-2-loaded cells, we demonstrated that alpha-thrombin (3-180 U l(-1)) induced a superimposable dose-dependent calcium transient in ECs from both strains. Fura-2 45-51 coagulation factor II Rattus norvegicus 93-101 10851228-2 2000 Stimulation of the particulate guanylate cyclase by brain-type natriuretic peptide in fura-2-loaded cells caused a profound potentiation of the ATP-stimulated and thapsigargin-stimulated rise in cytosolic free Ca(2+). Fura-2 86-92 natriuretic peptide B Homo sapiens 52-82 10913015-2 2000 We determined whether the VCAM-1-induced hyperpolarization is sufficient to augment the increase in intracellular free calcium ([Ca(2+)](i)) produced by Ca(2+) store depletion with thapsigargin (TG) and readdition of external CaCl(2) in fura 2-loaded THP-1 monocytes. Fura-2 237-243 vascular cell adhesion molecule 1 Homo sapiens 26-32 10919286-6 2000 Moreover, binding sites for endothelin-1 were increased, and the calcium response to the contractile agonist, determined in single fura-2-loaded cells, was enhanced. Fura-2 131-137 endothelin 1 Rattus norvegicus 28-40 11360670-2 2000 METHODS: The mobilization of cytosolic-free calcium induced by thrombin in washed platelets was observed by Ca(2+)-sensitive fluorescent indicator, Fura-2 AM and time scan measurement. Fura-2 148-154 prothrombin Oryctolagus cuniculus 63-71 10896718-16 2000 In fura-2-loaded cells, perforated patch whole-cell recordings show that ATP and UTP evoked slow outward currents at -60 mV, concomitant with the rise in [Ca2+]i, in approximately 30 % of rat intracardiac neurones. Fura-2 3-9 carbonic anhydrase 2 Rattus norvegicus 155-158 10837492-5 2000 Activation of G(q/11)-coupled receptors or receptor tyrosine kinases induced Mn(2+) entry in fura-2-loaded mTRPC4/5-expressing cells. Fura-2 93-99 transient receptor potential cation channel, subfamily C, member 4 Mus musculus 107-113 10742292-2 2000 The increase in the cytosolic Ca(2+) concentration ([Ca(2+)](i)) following repetitive stimulation with ATP or sphingosylphosphorylcholine (SPC) in single porcine aortic smooth muscle cells was investigated using the Ca(2+) indicator, fura-2. Fura-2 234-240 proline rich protein gene cluster Homo sapiens 139-142 10859594-5 2000 Using excitation wavelengths of lambda 340 nm (Fura-2) and lambda 485 nm (DAF-2) we could show that thrombin induces an intracellular calcium increase and simultaneously a NO formation in endothelial cells which could be blocked by a NO synthase inhibitor. Fura-2 47-53 coagulation factor II, thrombin Homo sapiens 100-108 10835036-3 2000 In rat primary astrocytes, short-term stimulation with thrombin resulted in a single [Ca2+]i transient and a dose-dependent de- and resensitisation, as assessed by single-cell Ca2+ imaging of fura-2-loaded astrocytes. Fura-2 192-198 coagulation factor II Rattus norvegicus 55-63 10792362-3 2000 METHODS: [Ca2+]i responses to PAF were examined in human eosinophils using a microscopic fura-2 fluorescence-ratio imaging system. Fura-2 89-95 PCNA clamp associated factor Homo sapiens 30-33 10642570-11 2000 Using the Ca(2+)-sensitive fluorescent indicator dye, fura-2, and digital imaging videomicroscopy, we found that PTH-rp (1 microM) stimulated intracellular free calcium ion concentrations ([Ca(2+)](i)) in single porcine theca cells. Fura-2 54-60 parathyroid hormone like hormone Homo sapiens 113-119 10771106-2 2000 The effect of anti-calreticulin antibody on intracellular free calcium concentration [Ca(2+)]i was studied in cultured neurons using fura-2 based microfluorometry. Fura-2 133-139 calreticulin Homo sapiens 19-31 10696094-1 2000 The mechanism of endothelium-dependent regulation of vascular tone of bradykinin was investigated by simultaneously monitoring the changes in the cytosolic Ca(2+) concentration and the force of smooth muscle in fura-2-loaded strips of the porcine renal artery with endothelium. Fura-2 211-217 kininogen 1 Homo sapiens 70-80 10681455-9 2000 Among the thrombin concentrations tested, 50 pM induced intracellular Ca(2+) spikes in fura-2-loaded CA1 neurons whereas higher concentrations caused a sustained Ca(2+) elevation. Fura-2 87-93 coagulation factor II Rattus norvegicus 10-18 10681455-9 2000 Among the thrombin concentrations tested, 50 pM induced intracellular Ca(2+) spikes in fura-2-loaded CA1 neurons whereas higher concentrations caused a sustained Ca(2+) elevation. Fura-2 87-93 carbonic anhydrase 1 Rattus norvegicus 101-104 10686291-1 2000 The regulatory mechanism of thrombin receptor responsiveness in in situ endothelial cells was investigated by evaluating elevations of cytosolic Ca(2+) concentration ([Ca(2+)](i)) in fura-2-loaded porcine aortic valvular strips. Fura-2 183-189 coagulation factor II, thrombin Homo sapiens 28-36 10704748-1 2000 The effects of the pro-inflammatory cytokine interleukin-1-beta (IL-1beta) on levels of intracellular calcium [Ca(2+)](i) in cultured human microglia have been studied using the fluorescent Ca(2+) indicator fura-2. Fura-2 207-213 interleukin 1 beta Homo sapiens 65-73 10670838-3 2000 Circulating PMN were isolated during 12 days, followed by determination of formyl-methionyl-leucyl-phenylalanine (fMLP)-induced PMN-superoxide production (PMN-SOP) by SOD-inhibitable ferricytochrome C reduction, and PMN cytosolic Ca2+ concentration ([Ca2+]i) by fluorescent fura2/AM (340/380 ratio). Fura-2 274-279 formyl peptide receptor 1 Homo sapiens 114-118 10640725-3 2000 Using fura- 2 Ca2+ imaging of murine Th1 and Th2 clones, we observed that the Ca2+ rise elicited following store depletion with thapsigargin is significantly lower in Th2 cells than in Th1 cells. Fura-2 6-13 negative elongation factor complex member C/D, Th1l Mus musculus 37-40 10640725-3 2000 Using fura- 2 Ca2+ imaging of murine Th1 and Th2 clones, we observed that the Ca2+ rise elicited following store depletion with thapsigargin is significantly lower in Th2 cells than in Th1 cells. Fura-2 6-13 heart and neural crest derivatives expressed 2 Mus musculus 167-170 10515989-4 1999 We investigated postnatal development of intracellular calcium dynamics in the proximal apical dendrites of CA1 pyramidal cells by whole cell patch-clamp recordings and calcium imaging with the Ca(2+) indicator fura-2. Fura-2 211-217 carbonic anhydrase 1 Rattus norvegicus 108-111 10616806-6 2000 Functional significance of thrombin receptors was indicated by changes in [Ca2+]i in response to thrombin, as measured by FURA-2 fluorescence in RBCE cells. Fura-2 122-128 coagulation factor II Rattus norvegicus 27-35 10616806-6 2000 Functional significance of thrombin receptors was indicated by changes in [Ca2+]i in response to thrombin, as measured by FURA-2 fluorescence in RBCE cells. Fura-2 122-128 coagulation factor II Rattus norvegicus 97-105 10620186-10 2000 Confocal [Ca2+]i measurements using fura-2 or fluo-3 or fluo-4 on the single cell level show that some of the Ang II-mediated [Ca2+]i response originated from podocytes. Fura-2 36-42 angiotensinogen Rattus norvegicus 110-116 10564232-1 1999 Bradykinin (BK)-induced changes in intracellular calcium level ([Ca(2+)](i)) were studied on fura 2-loaded afferent (AA) and efferent glomerular arterioles (EA) microdissected from juxtamedullary renal cortex. Fura-2 93-99 kininogen 1 Homo sapiens 0-10 10564232-1 1999 Bradykinin (BK)-induced changes in intracellular calcium level ([Ca(2+)](i)) were studied on fura 2-loaded afferent (AA) and efferent glomerular arterioles (EA) microdissected from juxtamedullary renal cortex. Fura-2 93-99 kininogen 1 Homo sapiens 12-14 10532691-4 1999 Furthermore, ET-1 stimulated protein synthesis (maximum 73 +/- 32% increase in [3H]leucine incorporation by 10(-7) M (P < 0.05)), as well as triggering intracellular calcium transients in human cSMC, as visualised under fura-2 fluorescence microscopy. Fura-2 223-229 endothelin 1 Homo sapiens 13-17 10799769-10 2000 Competitive receptor binding techniques using brain tissue sections as well as a fura-2 loaded primary cell culture system of the subfornical organ and organum vasculosum laminae terminalis demonstrate glial expression of functional ET(A) and ET(B) receptors, with calcium as intracellular messenger emerging primarily from intracellular stores. Fura-2 81-87 endothelin receptor type A Rattus norvegicus 233-238 11061984-3 2000 Ca(2+)fluxes in FMLP-activated neutrophils were measured using a fura-2/AM spectrofluorimetric procedure, while a colourimetric method was used to measure release of the primary granule enzyme, elastase, from the cells. Fura-2 65-71 formyl peptide receptor 1 Homo sapiens 16-20 10601493-7 1999 Fura-2 measurements revealed a biphasic increase in [Ca2+]i when 200 microM ACPD was bath applied to cultured hippocampal neurones. Fura-2 0-6 carbonic anhydrase 2 Rattus norvegicus 53-56 10516641-2 1999 The novel antimigraineur, dotarizine (30 microM), increased cytosolic Ca2+ concentration, [Ca2+]c, in fura-2-loaded bovine adrenal chromaffin cells. Fura-2 102-108 carbonic anhydrase 2 Bos taurus 70-73 10535385-6 1999 Experiments performed on cytochalasin D-treated cells using Mn2+ as an indicator of Ca2+ influx quenched fura-2 fluorescence signals following vasopressin administration. Fura-2 105-111 arginine vasopressin Rattus norvegicus 143-154 10629863-1 1999 To clarify Ca2+ entry channels involved in the endothelin-1 (ET-1)-induced increase in the intracellular concentration ([Ca2+]i), we performed whole-cell recordings of patch-clamp techniques and monitoring of [Ca2+]i with Ca2+ indicators fura-2 and fluo-3 in A7r5 cells (a cell line derived from rat thoracic aortic smooth muscle cells). Fura-2 238-244 endothelin 1 Rattus norvegicus 47-59 10629863-1 1999 To clarify Ca2+ entry channels involved in the endothelin-1 (ET-1)-induced increase in the intracellular concentration ([Ca2+]i), we performed whole-cell recordings of patch-clamp techniques and monitoring of [Ca2+]i with Ca2+ indicators fura-2 and fluo-3 in A7r5 cells (a cell line derived from rat thoracic aortic smooth muscle cells). Fura-2 238-244 endothelin 1 Rattus norvegicus 61-65 10513576-2 1999 Ni2+ (1 mM) significantly inhibited the transient increase in intracellular Ca2+ concentration ([Ca2+]i) induced by Ang II (100 nM) in aortic smooth muscle cells, as measured using fura-2. Fura-2 181-187 angiotensinogen Rattus norvegicus 116-122 10509892-1 1999 The calcium responses to serotonin and thrombin, as assessed using the fluorescent indicator Fura-2, have been investigated in platelets taken from 59 non-smokers and 17 smokers. Fura-2 93-99 coagulation factor II, thrombin Homo sapiens 39-47 10628349-3 1999 The use of various spectroscopic methods (fluorescence spectroscopy with Fura-2/AM on viable cells, 13C NMR spectroscopy on lipid extracts) reveals osmotic and metabolic changes in HePC treated sensitive cells. Fura-2 73-79 hepcidin antimicrobial peptide Homo sapiens 181-185 10510460-9 1999 4 E-1/E-2 (1 microM) increased [Ca2+]i in fura-2 loaded CHOmu cell suspensions in a thapsigargin sensitive and naloxone reversible manner. Fura-2 42-48 small nucleolar RNA, H/ACA box 73A Homo sapiens 2-9 10469354-2 1999 METHODS: We examined the distribution of aminopeptidase A (APA) activity by using a specific APA inhibitor and by immunostaining with an antirat kidney APA antibody, the Ang III-induced variations of [Ca2+]i by using fura-2 and the characterization of the receptor subtype involved in the response to Ang III in cortical thick ascending limb (CTAL). Fura-2 217-223 glutamyl aminopeptidase Rattus norvegicus 59-62 10370087-3 1999 In T84 cells grown on impermeable supports, BK stimulated a transient increase in [Ca2+]i as assessed by fura-2 ratio imaging. Fura-2 105-111 kininogen 1 Homo sapiens 44-46 10413074-6 1999 In cultured rat aortic smooth muscle cells loaded with fura-2, increases in intracellular free-Ca2+ concentration ([Ca2+]i) induced by angiotensin II were markedly inhibited in cells incubated with simvastatin and atorvastatin but not pravastatin. Fura-2 55-61 angiotensinogen Rattus norvegicus 135-149 10375648-1 1999 To understand the mechanism(s) underlying the Cd2+- and Co2+-induced increases in the cytosolic free Ca2+ concentration ([Ca]i) in cat adrenal chromaffin cells, we used nystatin-perforated patch recording method and fura-2 microfluorometry. Fura-2 216-222 CD2 molecule Canis lupus familiaris 46-49 10375648-5 1999 The Cd2+-induced inward current was activated in a concentration-dependent manner with a half-maximum concentration of 9.3x10(-8) M. The Cd2+- and Co2+-induced [Ca]i increases measured with fura-2 microfluorometry were maximal at 10(-6) and 10(-5) M, respectively, and the higher concentrations of both cations caused the smaller responses. Fura-2 190-196 CD2 molecule Canis lupus familiaris 4-7 10375648-5 1999 The Cd2+-induced inward current was activated in a concentration-dependent manner with a half-maximum concentration of 9.3x10(-8) M. The Cd2+- and Co2+-induced [Ca]i increases measured with fura-2 microfluorometry were maximal at 10(-6) and 10(-5) M, respectively, and the higher concentrations of both cations caused the smaller responses. Fura-2 190-196 CD2 molecule Canis lupus familiaris 137-140 10336664-1 1999 The properties of the vestibular Na+-Ca2+ exchanger in mammalian type I vestibular sensory cells were studied using fura-2 fluorescence and immunocytochemical techniques. Fura-2 116-122 nascent polypeptide associated complex subunit alpha 2 Homo sapiens 33-40 10340744-1 1999 The effect of chemical anoxia (azide) in the presence of glucose on the free intracellular Ca2+ concentration ([Ca2+]i) and intracellular pH (pHi) in mouse neocortical neurons was investigated using Fura-2 and BCECF. Fura-2 199-205 glucose-6-phosphate isomerase 1 Mus musculus 142-145 10320025-6 1999 In Fura-2-loaded intracardiac neurones, Met-enkephalin evoked a transient increase in [Ca2+]i. Fura-2 3-9 proopiomelanocortin Homo sapiens 40-54 10209009-4 1999 Effects of bradykinin and ramiprilat on intracellular signaling were determined by monitoring the activation of the extracellularly regulated kinases Erk1 and Erk2 as well as [Ca2+]i increases in fura 2-loaded endothelial cells. Fura-2 196-202 kininogen 1 Homo sapiens 11-21 10217297-1 1999 The influx of the toxic cation Cd2+ was studied in fura 2-loaded rat cerebellar granule neurons. Fura-2 51-57 Cd2 molecule Rattus norvegicus 31-34 10191330-6 1999 Digital-imaging physiological studies with fura-2 detected a rise in intracellular calcium in response to hypocretin in cultured rat spinal cord neurons, indicating that spinal cord neurons express hypocretin-responsive receptors. Fura-2 43-49 hypocretin Mus musculus 106-116 10191330-6 1999 Digital-imaging physiological studies with fura-2 detected a rise in intracellular calcium in response to hypocretin in cultured rat spinal cord neurons, indicating that spinal cord neurons express hypocretin-responsive receptors. Fura-2 43-49 hypocretin Mus musculus 198-208 10098511-12 1999 [Ca2+]i moderately augmented the response to hCG in fura-2/AM-loaded mLTC-1 cells within 30-40 sec, reaching a plateau within 1-3 min. Fura-2 52-58 hypertrichosis 2 (generalised, congenital) Homo sapiens 45-48 10201687-3 1999 METHODS: Changes in [Ca2+]i were measured using fura-2 fluorescence spectroscopy in rat cortical brain slices at 90, 185, 370, and 705 microM isoflurane. Fura-2 48-54 carbonic anhydrase 2 Rattus norvegicus 21-24 10082975-0 1999 Measurements of [Ca2+] using fura-2 in glioma C6 cells expressing calretinin with GFP as a marker of transfection: no Ca2+-buffering provided by calretinin. Fura-2 29-35 calbindin 2 Homo sapiens 66-76 10066929-2 1999 The Ca2+-sensitive fluorescent indicator rhod-2 was used to measure mitochondrial [Ca2+] ([Ca2+]m) in single smooth muscle cells from the rat pulmonary artery, while simultaneously monitoring cytosolic [Ca2+] ([Ca2+]i) with fura-2. Fura-2 224-230 carbonic anhydrase 2 Rattus norvegicus 4-7 10100864-1 1999 Caffeine mobilized an intracellular Ca2+ pool in intact fura-2-loaded INS-1 cells in suspension exposed to high (16 mM) [glucose], while a minor effect was observed with low (2 mM) [glucose]. Fura-2 56-62 insulin 1 Rattus norvegicus 70-75 10188626-1 1999 The elevation of intracellular [Ca2+] induced by bradykinin (Bk) was monitored with fura-2 fluorescence in human skin fibroblasts. Fura-2 84-90 kininogen 1 Homo sapiens 49-59 10226172-10 1999 In fura-2-loaded pancreatic acinar cells Ins or Glu evoked small increases in [Ca2+]i compared with a much larger elevation with CCK-8. Fura-2 3-9 cholecystokinin Rattus norvegicus 129-132 10090417-4 1999 The mechanisms of angiotensin II-evoked calcium signaling were examined using fura-2-based fluorescent digital microscopy. Fura-2 78-84 angiotensinogen Rattus norvegicus 18-32 10188626-1 1999 The elevation of intracellular [Ca2+] induced by bradykinin (Bk) was monitored with fura-2 fluorescence in human skin fibroblasts. Fura-2 84-90 kininogen 1 Homo sapiens 61-63 9852560-8 1998 Fura-2 recordings showed that BDNF elicited an increase in intracellular calcium concentration ([Ca2+]c). Fura-2 0-6 brain derived neurotrophic factor Homo sapiens 30-34 9930728-7 1999 PGE2 and the EP1 agonist 17-phenyl trinor PGE2 but not the EP3 agonist sulprostone elicited reversible intracellular [Ca2+] increases in microglia as measured by fura-2. Fura-2 162-168 prostaglandin E receptor 1 Rattus norvegicus 13-16 10051189-2 1999 In the present report, changes in the intracellular Ca2+ concentration in the hippocampal CA1 area during transient global ischemia and reperfusion were measured using in vivo Ca2+ fluorometry with fura-2 in the four-vessel occlusion and reperfusion model in halothane-anesthetized rats. Fura-2 198-204 carbonic anhydrase 2 Rattus norvegicus 52-55 10051189-2 1999 In the present report, changes in the intracellular Ca2+ concentration in the hippocampal CA1 area during transient global ischemia and reperfusion were measured using in vivo Ca2+ fluorometry with fura-2 in the four-vessel occlusion and reperfusion model in halothane-anesthetized rats. Fura-2 198-204 carbonic anhydrase 1 Rattus norvegicus 90-93 10051189-2 1999 In the present report, changes in the intracellular Ca2+ concentration in the hippocampal CA1 area during transient global ischemia and reperfusion were measured using in vivo Ca2+ fluorometry with fura-2 in the four-vessel occlusion and reperfusion model in halothane-anesthetized rats. Fura-2 198-204 carbonic anhydrase 2 Rattus norvegicus 176-179 10064008-5 1999 ATP and alpha,beta-methylene ATP, a specific agonist for P2X1 receptors, at micromolar concentrations also increased Mn2+ quench, but to lesser extents than did ADP, in SPD platelets isolated and loaded with fura-2 in the presence of apyrase. Fura-2 208-214 purinergic receptor P2X 1 Homo sapiens 57-61 10064008-3 1999 With thrombin, this enhanced [Ca2+]i decay was associated with decreased Ca2+ influx, as measured by Mn2+ quench of fura-2 fluorescence. Fura-2 116-122 coagulation factor II, thrombin Homo sapiens 5-13 10211022-4 1998 Concentrations of [Ca2+]i in the pyramidal cell layer of the CA1 region were measured using a calcium indicator dye, fura-2. Fura-2 117-123 carbonic anhydrase 2 Rattus norvegicus 19-22 9837897-6 1998 Analysis of the properties of recombinant RyR2 and RyR1 by measurement of intracellular Fura-2 fluorescence revealed that the different properties of RyR2 and RyR1 are retained in the recombinant expressed proteins. Fura-2 88-94 ryanodine receptor 2 Oryctolagus cuniculus 42-46 9837897-6 1998 Analysis of the properties of recombinant RyR2 and RyR1 by measurement of intracellular Fura-2 fluorescence revealed that the different properties of RyR2 and RyR1 are retained in the recombinant expressed proteins. Fura-2 88-94 ryanodine receptor 1 Oryctolagus cuniculus 51-55 9837897-6 1998 Analysis of the properties of recombinant RyR2 and RyR1 by measurement of intracellular Fura-2 fluorescence revealed that the different properties of RyR2 and RyR1 are retained in the recombinant expressed proteins. Fura-2 88-94 ryanodine receptor 1 Oryctolagus cuniculus 159-163 9832138-5 1998 We found that fura-2 was useful in measuring small increases in [Zn2+]i associated with exposure to Zn2+ alone that may be mediated by a Na+/Ca2+ exchanger. Fura-2 14-20 solute carrier family 8 member A1 Homo sapiens 137-155 9808762-2 1998 Immunoblot analysis revealed that A7r5 vascular smooth muscle cells expressed conventional (alpha), novel (delta and epsilon), and atypical (iota/lambda and mu) isoforms of protein kinase C. Stimulation of fura-2-loaded cells with 20 nM vasopressin induced a rapid transient increase in the intracellular concentration of calcium that was followed by a slowly declining component which was above baseline throughout the period of observation. Fura-2 206-212 arginine vasopressin Rattus norvegicus 237-248 9826729-3 1998 Fura-2-based Ca imaging showed that numerous chemokines, including SDF-1alpha, RANTES, and fractalkine, affect neuronal Ca signaling, suggesting that hippocampal neurons possess a wide variety of chemokine receptors. Fura-2 0-6 C-C motif chemokine ligand 5 Homo sapiens 79-85 9826729-3 1998 Fura-2-based Ca imaging showed that numerous chemokines, including SDF-1alpha, RANTES, and fractalkine, affect neuronal Ca signaling, suggesting that hippocampal neurons possess a wide variety of chemokine receptors. Fura-2 0-6 C-X3-C motif chemokine ligand 1 Homo sapiens 91-102 9804623-8 1998 By using the fura-2 fluorescence method, a difference in the kinetics of Ca2+ flux with Edg2 and Edg4 was observed. Fura-2 13-19 lysophosphatidic acid receptor 1 Homo sapiens 88-92 9813161-2 1998 We measured the activity of nitrendipine-sensitive Ca2+ channels of intact cardiac myocytes in the presence of IGF1 by monitoring unidirectional Mn2+ influx measured as the quench of cytosolic fura-2 in electrically stimulated or K+-depolarized cells. Fura-2 193-199 insulin like growth factor 1 Homo sapiens 111-115 9815040-10 1998 In fura 2-loaded, purified parietal cells, 10 min preincubation with IL-1beta dramatically reduced the initial transient peak elevation of intracellular Ca2+ concentration in response to carbachol. Fura-2 3-9 interleukin 1 beta Rattus norvegicus 69-77 9804623-8 1998 By using the fura-2 fluorescence method, a difference in the kinetics of Ca2+ flux with Edg2 and Edg4 was observed. Fura-2 13-19 lysophosphatidic acid receptor 2 Homo sapiens 97-101 9742162-8 1998 Because NMDA receptor activation leads to increased intracellular Ca2+ concentration ([Ca2+]i), we studied the effect of the BDNF- and FGF2-induced reduction in NR2A and NR2C synthesis on the NMDA-evoked Ca2+ responses by single-cell fura-2 fluorescence ratio imaging. Fura-2 234-240 fibroblast growth factor 2 Rattus norvegicus 135-139 9770374-4 1998 Free cytosolic Ca2+ was measured with the use of fluorescent dye Fura-2 during PLA2 agonist/antagonist studies. Fura-2 65-71 phospholipase A2 group IB Homo sapiens 79-83 9849678-2 1998 Simultaneous measurements of kainate-activated Ca2+ fluxes and inward currents, using fura-2 microfluorimetry under voltage clamp conditions, suggested the existence of GluR2 containing channels which are permeable to Ca2+ and insensitive to Joro spider toxin (JSTx). Fura-2 86-92 glutamate ionotropic receptor AMPA type subunit 2 Homo sapiens 169-174 9767522-3 1998 METHODS: LPS- or BK-induced increments of intracellular Ca2+ were assessed by microspectrofluorimetry with fura-2 in passaged bovine aortic endothelial cells. Fura-2 107-113 kininogen 1 Bos taurus 17-19 11717867-2 1998 METHODS: The fura-2 microfluorimetry was used to measure changes of [Ca2+]i in OHCs of the guinea pig cochlea after application of acetylcholine, ATP and carbacholine. Fura-2 13-19 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 69-72 9920348-0 1998 Changes in cytosolic Ca2+ measured by use of fura-2 and contraction produced by quick stretch and various stimulants in canine cerebral artery. Fura-2 45-51 carbonic anhydrase 2 Canis lupus familiaris 21-24 9873837-7 1998 In order to identify what triggers the PLA2 activation, [Ca2+]i was measured by using a fluorescence dye, Fura-2. Fura-2 106-112 phospholipase A2 group IB Canis lupus familiaris 39-43 9736645-4 1998 Fura-2 fixation combined with immunohistochemistry revealed that fura-2-loaded cells were GFAP+/O4(-) and were therefore a population of neonatal white matter astrocytes. Fura-2 0-6 glial fibrillary acidic protein Rattus norvegicus 90-94 9714858-4 1998 Ratio imaging with fura-2 revealed moderate dendritic [Ca2+] increases (approximately 500 nM) during only the initial approximately 30 s of the 7.5 min stimulation period. Fura-2 19-25 carbonic anhydrase 2 Rattus norvegicus 55-58 9736645-4 1998 Fura-2 fixation combined with immunohistochemistry revealed that fura-2-loaded cells were GFAP+/O4(-) and were therefore a population of neonatal white matter astrocytes. Fura-2 65-71 glial fibrillary acidic protein Rattus norvegicus 90-94 9694886-2 1998 In this study, we examined the role of PKC in regulating the coupling of the CaR to Ca2+i dynamics in fura-2-loaded human embryonic kidney cells (HEK293 cells) transiently transfected with the human parathyroid CaR. Fura-2 102-108 proline rich transmembrane protein 2 Homo sapiens 39-42 9760037-2 1998 Bradykinin evoked a [Ca2+]i transient in a dose-dependent manner, measured by fura-2 fluorimetry and digital video imaging. Fura-2 78-84 kininogen 1 Canis lupus familiaris 0-10 9679168-6 1998 The measurements of changes in pHi evoked by changes in pHo were employed in subsequent experiments to correct for the effects of changes in pHi on the Kd of fura-2 for Ca2+. Fura-2 158-164 glucose-6-phosphate isomerase Rattus norvegicus 141-144 9685362-7 1998 Only phospholipase C-beta1 is involved in the mobilization of Ca2+ from the endoplasmic reticulum of Fura-2-loaded confluent OHS-4 cells and the formation of inositol 1,4,5-trisphosphate by CGRP; PLC-gamma have no effect. Fura-2 101-107 phospholipase C beta 1 Homo sapiens 5-26 9685362-7 1998 Only phospholipase C-beta1 is involved in the mobilization of Ca2+ from the endoplasmic reticulum of Fura-2-loaded confluent OHS-4 cells and the formation of inositol 1,4,5-trisphosphate by CGRP; PLC-gamma have no effect. Fura-2 101-107 calcitonin related polypeptide alpha Homo sapiens 190-194 9687496-6 1998 Ca2+ store depletion with thapsigargin induced TRP5-mediated calcium entry dependent on the concentration of extracellular calcium, as seen by dual wavelength fura-2 fluorescence ratio measurements. Fura-2 159-165 carbonic anhydrase 2 Homo sapiens 0-3 9687496-6 1998 Ca2+ store depletion with thapsigargin induced TRP5-mediated calcium entry dependent on the concentration of extracellular calcium, as seen by dual wavelength fura-2 fluorescence ratio measurements. Fura-2 159-165 transient receptor potential cation channel subfamily C member 5 Homo sapiens 47-51 9722316-2 1998 Using fura-2-loaded platelets from 51 healthy volunteers, high variability was detected in the Ca2+ responses evoked by the receptor agonists, thrombin and collagen, and the inhibitor of sarco/endoplasmic reticulum Ca2+-ATPases (SERCA), thapsigargin (Tg). Fura-2 6-12 coagulation factor II, thrombin Homo sapiens 143-151 9793689-1 1998 In human U373 MG astrocytoma cells, histamine and substance P stimulated similar peak increases in intracellular free calcium concentrations ([Ca2+]i), as measured by single cell imaging of Fura-2 fluorescence. Fura-2 190-196 tachykinin precursor 1 Homo sapiens 50-61 9749756-6 1998 In single-cell Fura-2 measurements, bFGF elicited a long lasting rise of the cytosolic calcium concentration that was dependent on [Ca2+]o. Fura-2 15-21 fibroblast growth factor 2 Gallus gallus 36-40 9871763-2 1998 The hNK3 ligands 3 antagonise the calcium mobilisation caused by activation of hNK3 receptors expressed in CHO cells as measured using fura-2 microspectrofluorimetry. Fura-2 135-141 NK3 homeobox 1 Homo sapiens 4-8 9698207-1 1998 The mechanism of thrombin-induced endothelium-dependent relaxation was investigated using fura-2 front-surface fluorometry. Fura-2 90-96 coagulation factor II, thrombin Sus scrofa 17-25 9871763-2 1998 The hNK3 ligands 3 antagonise the calcium mobilisation caused by activation of hNK3 receptors expressed in CHO cells as measured using fura-2 microspectrofluorimetry. Fura-2 135-141 NK3 homeobox 1 Homo sapiens 79-83 9689597-2 1998 To characterize signal transduction mechanisms of Ang II, we examined changes in intracellular Ca2+ concentration ([Ca2+]i) of fura-2-loaded cultured adult rat cardiac fibroblasts by fluorescence photometry. Fura-2 127-133 angiotensinogen Rattus norvegicus 50-56 9720762-1 1998 In fura-2-labelled human platelets, the thiol oxidising agent diamide decreases the intracellular calcium response to thrombin and serotonin without affecting the basal calcium levels. Fura-2 3-9 coagulation factor II, thrombin Homo sapiens 118-126 9519747-4 1998 Using the Fura-2 technique, application of either 1 nmol/l bradykinin or 1 micromol/l ATP to the normoglycemic endothelial cells led to a significant increase in intracellular calcium, whereas the hyperglycemic cells showed significantly less reactivity to both agents. Fura-2 10-16 kininogen 1 Homo sapiens 59-69 9610724-4 1998 Using the fluorescent probe Fura-2, we assayed the cytoplasmic calcium levels in human cancer cells stimulated with anti-Trop-2 or control antibodies. Fura-2 28-34 tumor associated calcium signal transducer 2 Homo sapiens 121-127 9494083-2 1998 Rates of Ca2+ inflow were estimated by measuring the increase in the fluorescence of intracellular fura-2 following the addition of extracellular Ca2+ (Ca2+o) to cells incubated in the absence of added Ca2+o. Fura-2 99-105 carbonic anhydrase 2 Rattus norvegicus 9-12 9494083-2 1998 Rates of Ca2+ inflow were estimated by measuring the increase in the fluorescence of intracellular fura-2 following the addition of extracellular Ca2+ (Ca2+o) to cells incubated in the absence of added Ca2+o. Fura-2 99-105 carbonic anhydrase 2 Rattus norvegicus 146-149 9494083-2 1998 Rates of Ca2+ inflow were estimated by measuring the increase in the fluorescence of intracellular fura-2 following the addition of extracellular Ca2+ (Ca2+o) to cells incubated in the absence of added Ca2+o. Fura-2 99-105 carbonic anhydrase 2 Rattus norvegicus 146-149 9494083-2 1998 Rates of Ca2+ inflow were estimated by measuring the increase in the fluorescence of intracellular fura-2 following the addition of extracellular Ca2+ (Ca2+o) to cells incubated in the absence of added Ca2+o. Fura-2 99-105 carbonic anhydrase 2 Rattus norvegicus 146-149 9461611-7 1998 Analysis of NCKX2 function by fluorescent imaging of fura-2-loaded transfected cells demonstrated that NCKX2 is a potassium-dependent sodium/calcium exchanger. Fura-2 53-59 solute carrier family 24 member 2 Rattus norvegicus 12-17 9582501-7 1998 In fura-2 loaded BAG cells, BK (100 nM) induced a typical biphasic Ca2+ response composed of a rapid and transient increase of intracellular Ca2+ concentration [Ca2+]i which slowly declined to a level that remained above basal level for about 5 min. Fura-2 3-9 kininogen 1 Bos taurus 28-30 9464995-3 1998 Extracellularly applied ATP (50 microM) evoked fast [Ca2+]i rises revealed by a rapid and transient increase in fura-2 F340/F380 ratio in all Purkinje cells tested, whereas granule cells failed to show a response to ATP. Fura-2 112-118 carbonic anhydrase 2 Rattus norvegicus 53-56 9461611-7 1998 Analysis of NCKX2 function by fluorescent imaging of fura-2-loaded transfected cells demonstrated that NCKX2 is a potassium-dependent sodium/calcium exchanger. Fura-2 53-59 solute carrier family 24 member 2 Rattus norvegicus 103-108 9502114-4 1998 Measurement of intracellular Ca2+ concentration ([Ca2+]i) by Fura-2 indicates that VIP gradually elevates [Ca2+]i, with the maximum level attained at 4 min following hormone addition. Fura-2 61-67 vasoactive intestinal peptide Rattus norvegicus 83-86 9417057-8 1998 Measurement of intracellular Ca2+ concentrations in Fura2-loaded cells showed that cell lines expressing hTrp3 have significantly higher basal and agonist-stimulated influxes of Ca2+, Mn2+, Ba2+, and Sr2+ than control cells. Fura-2 52-57 transient receptor potential cation channel subfamily C member 3 Homo sapiens 105-110 9490829-19 1998 The rate of Mn2+ quench of fura-2 fluorescence was increased by the inhibitors, and the inhibitors induced a small inward current when K+ channels were blocked that preceded the rise in [Ca2+]i. Fura-2 27-33 carbonic anhydrase 2 Rattus norvegicus 187-190 9490842-2 1998 By applying fura-2-based fluorometric calcium imaging to neonatal rat hippocampal slices we identified a developmentally regulated spontaneous neuronal activity in the CA1 region of the hippocampus. Fura-2 12-18 carbonic anhydrase 1 Rattus norvegicus 168-171 9481674-9 1998 The peak of the Ca2+ signal measured with fura-2 preceded peak 1 of force and then declined to a lower suprabasal steady-state level. Fura-2 42-48 pseudopodium-enriched atypical kinase 1 Cavia porcellus 58-64 9649922-7 1998 Transient increases in intracellular calcium concentration in cSMCs in response to angiotensin II stimulation were visualized under fura-2 fluorescence microscopy. Fura-2 132-138 angiotensinogen Homo sapiens 83-97 9421413-2 1998 In these cells, insulin evoked the elevation of cytoplasmic free calcium concentration, [Ca2+]c, measured by using fura-2. Fura-2 115-121 insulin Cricetulus griseus 16-23 9441706-1 1997 Regulation of intracellular free Ca2+ concentration ([Ca2+]i) by an Na+/Ca2+ exchanger was studied in cultures of human retinal pigment epithelial cells using Ca(2+)-indicator dyes (fura-2 and fluo-3) and digital fluorescence imaging. Fura-2 182-188 solute carrier family 8 member A1 Homo sapiens 68-86 9418702-7 1997 RESULT(S): All oocytes loaded with fura-2 and then exposed to ionophore exhibited an isolated elevation of [Ca]i, followed by prompt return to baseline levels. Fura-2 35-41 carbonic anhydrase 1 Homo sapiens 108-112 9444969-2 1998 In each strain the Ang II induced changes of [Ca2+]i were measured in cultured glomerular mesangial cells (MC) using the calcium-sensitive fluorescent dye, fura2. Fura-2 156-161 angiotensinogen Rattus norvegicus 19-25 9374730-3 1997 In human ASM cells loaded with fura 2, TNF-alpha and interleukin (IL)-1 beta significantly enhanced thrombin- and bradykinin-evoked elevations of intracellular Ca2+. Fura-2 31-37 tumor necrosis factor Homo sapiens 39-48 9423175-4 1997 PACAP release from islets was bioassayed using the response of cytosolic Ca2+ concentration ([Ca2+]i) in single beta-cells, monitored by dual-wavelength fura-2 microfluorometry. Fura-2 153-159 adenylate cyclase activating polypeptide 1 Rattus norvegicus 0-5 9368034-7 1997 Transient expression of mTrp6 in COS.M6 cells by transfection of the full-length mTrp6 cDNA increases Ca2+ entry induced by stimulation of co-transfected M5 muscarinic acetylcholine receptor with carbachol (CCh), as seen by dual wavelength fura 2 fluorescence ratio measurements. Fura-2 240-246 transient receptor potential cation channel, subfamily C, member 6 Mus musculus 24-29 9368034-7 1997 Transient expression of mTrp6 in COS.M6 cells by transfection of the full-length mTrp6 cDNA increases Ca2+ entry induced by stimulation of co-transfected M5 muscarinic acetylcholine receptor with carbachol (CCh), as seen by dual wavelength fura 2 fluorescence ratio measurements. Fura-2 240-246 transient receptor potential cation channel, subfamily C, member 6 Mus musculus 81-86 9374730-3 1997 In human ASM cells loaded with fura 2, TNF-alpha and interleukin (IL)-1 beta significantly enhanced thrombin- and bradykinin-evoked elevations of intracellular Ca2+. Fura-2 31-37 interleukin 1 beta Homo sapiens 53-76 9374730-3 1997 In human ASM cells loaded with fura 2, TNF-alpha and interleukin (IL)-1 beta significantly enhanced thrombin- and bradykinin-evoked elevations of intracellular Ca2+. Fura-2 31-37 coagulation factor II, thrombin Homo sapiens 100-108 9374730-3 1997 In human ASM cells loaded with fura 2, TNF-alpha and interleukin (IL)-1 beta significantly enhanced thrombin- and bradykinin-evoked elevations of intracellular Ca2+. Fura-2 31-37 kininogen 1 Homo sapiens 114-124 9481478-1 1997 The fluorescent indicator Fura-2 was used to characterize the store-operated Ca2+ entry in insulin-releasing pancreatic beta-cells. Fura-2 26-32 insulin Homo sapiens 91-98 9383042-4 1997 Using the ratiometric fluorescent indicator fura-2, we observed large spontaneous [Ca2+]i oscillations. Fura-2 44-50 carbonic anhydrase 2 Homo sapiens 83-86 9353354-6 1997 In cells loaded with Fura-2, atrial natriuretic peptide both augmented depolarization-induced increases of intracellular free calcium concentrations and accelerated the depolarization-induced quenching of the Fura-2 signal by manganese, findings consistent with enhanced conductivity of calcium channels. Fura-2 21-27 natriuretic peptide A Rattus norvegicus 29-55 9353354-6 1997 In cells loaded with Fura-2, atrial natriuretic peptide both augmented depolarization-induced increases of intracellular free calcium concentrations and accelerated the depolarization-induced quenching of the Fura-2 signal by manganese, findings consistent with enhanced conductivity of calcium channels. Fura-2 209-215 natriuretic peptide A Rattus norvegicus 29-55 9357760-3 1997 CaR agonists (Ca2+, poly-L-arginine, protamine) mediated an increase in intracellular Ca2+ in HT-29-18-C1 cells (monitored by changes in fura 2 fluorescence), which was dependent on release from thapsigargin-sensitive stores. Fura-2 137-143 calcium sensing receptor Homo sapiens 0-3 9277485-6 1997 VEGF also increased the intracellular calcium concentration in endothelial cells of perfused microvessels as determined by measurement with fura 2. Fura-2 140-146 vascular endothelial growth factor A Homo sapiens 0-4 9344760-3 1997 Compared with hypothyroid animals, myocytes isolated from hyperthyroid rat hearts demonstrated a significant: (1) increase in extent of shortening; (2) decrease in the time to peak contraction; (3) increase in the peak amplitude of the fura-2 fluorescence ratio; (4) decrease in pHi (DeltapHi=0. Fura-2 236-242 glucose-6-phosphate isomerase Rattus norvegicus 279-282 9385466-0 1997 Calibration of Fura-2 signals introduces errors into measurement of thrombin-stimulated calcium mobilisation in human platelets. Fura-2 15-21 coagulation factor II, thrombin Homo sapiens 68-76 9385466-1 1997 The intracellular calcium indicator dye Fura-2 has been widely used for the study of human platelet thrombin receptor-mediated calcium mobilisation in disease states. Fura-2 40-46 coagulation factor II, thrombin Homo sapiens 100-108 9385466-3 1997 In the present study, it is found (a) that a greater peak response to thrombin is seen when 1 mumol/l Fura-2/AM rather than 2 or 3 mumol/l is used; and (b) that calibration leads to a poorer test-retest reliability and in general a greater variability of the obtained calcium signal than when the simple measurement of the 340 nm/380 nm fluorescence ratio is used. Fura-2 102-108 coagulation factor II, thrombin Homo sapiens 70-78 9281578-1 1997 Extracellular ATP (ATPo) elicits a robust change in the concentration of intracellular Ca2+ ([Ca2+]i) in fura-2-loaded mouse thymocytes. Fura-2 105-111 ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit Mus musculus 19-23 9316451-2 1997 Presently, we used single-cell Ca2+ imaging to study the combined effects of 1,25(OH)2D3 and parathyroid hormone (PTH) during generation of Ca2+ transients in fura 2-loaded MC3T3-E1 cells. Fura-2 159-165 parathyroid hormone Mus musculus 93-112 9277347-1 1997 Muscarinic stimulation of fura 2-loaded smooth muscle cells evoked a rapidly inactivating Ca(2+)-activated Cl- current [ICl(Ca)] and a sustained nonselective cation current (Icat) as well as a transient (delta Ca(tran)) and a sustained (delta Ca(sus)) elevation of cytosolic Ca2+ concentration ([Ca2+]i). Fura-2 26-32 catenin beta interacting protein 1 Homo sapiens 174-178 9310305-1 1997 The intracellular free Ca2+ concentration ([Ca2+]i) in the CA1 pyramidal cell layer was measured using fura-2-loaded hippocampal slices prepared from adult rats. Fura-2 103-109 carbonic anhydrase 2 Rattus norvegicus 23-26 9310305-1 1997 The intracellular free Ca2+ concentration ([Ca2+]i) in the CA1 pyramidal cell layer was measured using fura-2-loaded hippocampal slices prepared from adult rats. Fura-2 103-109 carbonic anhydrase 2 Rattus norvegicus 44-47 9310305-1 1997 The intracellular free Ca2+ concentration ([Ca2+]i) in the CA1 pyramidal cell layer was measured using fura-2-loaded hippocampal slices prepared from adult rats. Fura-2 103-109 carbonic anhydrase 1 Rattus norvegicus 59-62 9321915-12 1997 To investigate the effect of stimulation of the CaR on intracellular Ca2+ (Ca2+i), we used the acetoxymethyl ester of fura 2 to monitor the Ca2+i. Fura-2 118-124 nuclear receptor subfamily 1, group I, member 3 Rattus norvegicus 48-51 9334884-1 1997 In Chinese hamster ovary cells stably expressing the cloned human cholecystokinin (CCK)B/ gastrin receptor, cholecystokinin octapeptide (CCK-8) evoked increases in [Ca2+]i monitored by digitized video imaging of fura-2 fluorescence ratios. Fura-2 212-218 cholecystokinin B receptor Homo sapiens 90-106 9277471-5 1997 Systolic fluorescence ratio using fura 2 as the Ca2+ indicator fell by 11.0%, and the decline of the fluorescence ratio was prolonged by 29.8%. Fura-2 34-40 carbonic anhydrase 2 Rattus norvegicus 48-51 9277471-9 1997 The relaxation abnormalities are most likely caused by altered Ca2+ handling, since we observed a reduction in the sarcoplasmic reticulum Ca2+ content and a prolongation of the decline of the fura 2 transient. Fura-2 192-198 carbonic anhydrase 2 Rattus norvegicus 63-66 9259111-5 1997 The free cytosolic Ca++ concentration, measured by Fura-2, increased after thrombin or TRAP stimulation in the cells expressing TR-1. Fura-2 51-57 coagulation factor II Rattus norvegicus 75-83 9263987-13 1997 In the next step, [Ca2+]i changes were measured in single cells loaded with Fura-2 after microinjection of neutralizing PKC isoenzyme-specific antibodies. Fura-2 76-82 protein kinase C alpha Bos taurus 120-123 9271330-6 1997 In fura-2-loaded platelets, the elevation of intracellular free calcium concentration stimulated by AA, thrombin, and 4-bromo-A23187 was inhibited by NQ-Y15 in a concentration-dependent manner. Fura-2 3-9 coagulation factor II Rattus norvegicus 104-112 9222898-4 1997 In whole cell clamped myocytes dialyzed with 0.1-0.2 mM Fura-2, the magnitude of ICa and Ca2+i-transient triggered by ICa or caffeine were not significantly different in transgenic vs. control myocytes. Fura-2 56-62 inhibitor of carbonic anhydrase Mus musculus 118-121 9246571-8 1997 AT (1 microM) increased HUVEC intracellular calcium mobilization, as monitored by FURA-2, to 245 nM from control (70 nM), however, pre-treatment with either BK or the bradykinin receptor antagonist decreased the AT induced intracellular calcium mobilization compared to AT alone. Fura-2 82-88 kininogen 1 Homo sapiens 167-177 9222898-12 1997 In 0.1 mM Fura-2-dialyzed transgenic myocytes 100 microM Cd2+ effectively blocked ICa and suppressed Cai-transients at -10 or +50 mV. Fura-2 10-16 inhibitor of carbonic anhydrase Mus musculus 82-85 11596320-5 1997 Furthermore, Fura-2/AM assay was more reliable than MTT assay for screening MDR modulators because MTT assay was dependent on the viable cells, while Fura-2/AM assay was dependent on the function of P-gp. Fura-2 13-19 phosphoglycolate phosphatase Homo sapiens 199-203 11596320-5 1997 Furthermore, Fura-2/AM assay was more reliable than MTT assay for screening MDR modulators because MTT assay was dependent on the viable cells, while Fura-2/AM assay was dependent on the function of P-gp. Fura-2 150-156 phosphoglycolate phosphatase Homo sapiens 199-203 9174646-1 1997 We previously demonstrated that histamine and bradykinin evoke an increase in intracellular Ca2+ ([Ca2+]i) in human gingival fibroblasts by using a fluorescent Ca2+ indicator Fura-2. Fura-2 175-181 kininogen 1 Homo sapiens 46-56 9134961-6 1997 The Fura-2 assay was used to estimate whether low dosages of exogenous PAF affect cultured neurons. Fura-2 4-10 PCNA clamp associated factor Rattus norvegicus 71-74 9178189-6 1997 Changes in intracellular calcium ion concentration ([Ca2+]i) induced by FMLP (30 nM) were studied in fura-2-loaded human PMN. Fura-2 101-107 formyl peptide receptor 1 Homo sapiens 72-76 9136085-5 1997 In suspended cells loaded with the calcium indicator dye fura-2, pretreatment with PKC inhibitors calphostin C (100 nM x 1 h) and H-7 (30 microM x 18 h) potentiated the effects of 1 microgram/ml bPTH (1-84) on Cai2+ (83% increase over basal) by 1.4- and 1.65-fold, respectively. Fura-2 57-63 protein kinase C, gamma Rattus norvegicus 83-86 9134961-8 1997 After preincubation for 120 min, the Fura-2-loaded neurons were exposed to various concentrations of PAF for 60 min. Fura-2 37-43 PCNA clamp associated factor Rattus norvegicus 101-104 9134961-9 1997 By measuring the fluorescent intensity of the medium as representing the amount of Fura-2 released from damaged neurons, we detected an increased release of Fura-2, even at low doses of PAF (P < 0.01 at 10 nM PAF). Fura-2 157-163 PCNA clamp associated factor Rattus norvegicus 212-215 9017193-6 1997 The relationship between the [Ca2+]t measured with furaptra and the postsynaptic response was the same as previously observed with fura-2: the postsynaptic response was proportional to about the fourth power (m approximately 4) of the amplitude of either [Ca2+]t or calcium influx. Fura-2 131-137 carbonic anhydrase 2 Homo sapiens 30-33 9045728-10 1997 We also show that Ca2+ influx into hippocampal neurons in response to cyclic nucleotide elevation can be detected using fura-2 imaging. Fura-2 120-126 carbonic anhydrase 2 Rattus norvegicus 18-21 9071214-4 1997 Changes in intracellular [Ca2+] ([Ca2+]i) were measured on fura-2-loaded acini in response to VIP. Fura-2 59-65 vasoactive intestinal peptide Rattus norvegicus 94-97 9105731-1 1997 Ca2+ transients evoked by endothelin-1 (ET-1) were measured in single cells of an immortalized human tracheal epithelial cell line using Fura-2. Fura-2 137-143 endothelin 1 Homo sapiens 40-44 9065736-2 1997 Using fura-2-loaded cells, we observed that the release induced by AVP occurred concurrently with calcium (Ca2+) mobilization from internal stores and entry of external Ca2+, whereas AlF4(-)-dependent arachidonate release was much slower and was not accompanied by intracellular Ca2+ mobilization. Fura-2 6-12 arginine vasopressin Rattus norvegicus 67-70 8986232-10 1996 HGF-induced changes in [Ca2+]i were measured in single fura 2-loaded hepatocytes by fluorescence imaging techniques. Fura-2 55-61 hepatocyte growth factor Rattus norvegicus 0-3 9056076-1 1997 In cultured endothelial cells, the temperature dependence of bradykinin-initiated Ca2+ signaling was studied using Fura-2 technique. Fura-2 115-121 kininogen 1 Homo sapiens 61-71 9170163-7 1997 Acute addition of fibronectin to coverslips of uveal melanocytes loaded with Fura-2 produced an acute and transient increase in intracellular calcium which was more prevalent in low density than higher density cells. Fura-2 77-83 fibronectin 1 Homo sapiens 18-29 10453550-3 1997 When verapamil, EG-TA, dantrolene, and Fura-2/AM, were added to the medium, the 3H-leucine incorporations stimulated by Ang II were reduced by 17%, 19%, 22%, and 27%. Fura-2 39-45 angiotensinogen Homo sapiens 120-126 9007535-2 1996 Using ratio imaging with fura-2, we demonstrate that the induction of this long-term depression is associated with a rapid and transient (approximately 30 s) dendritic Ca2+ increase (approximately 500 nM) dependent on the activation of voltage-gated Ca2+ channels. Fura-2 25-31 carbonic anhydrase 2 Rattus norvegicus 168-171 9007535-2 1996 Using ratio imaging with fura-2, we demonstrate that the induction of this long-term depression is associated with a rapid and transient (approximately 30 s) dendritic Ca2+ increase (approximately 500 nM) dependent on the activation of voltage-gated Ca2+ channels. Fura-2 25-31 carbonic anhydrase 2 Rattus norvegicus 250-253 8908321-4 1996 Problems of mutual interference of Ca2+ and Cd2+ analysis with the fluorescent probe Fura-2 could be overcome by the use of the fluorine 19 nuclear magnetic resonance (19F-NMR) probe acetoxymethyl ester of 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N"N"-tetraacetic acid (5F-BAPTA), which allows the measurement of free intracellular Ca2+, Cd2+ and other metal ions concurrently. Fura-2 85-91 Cd2 molecule Rattus norvegicus 44-47 8997399-1 1996 This study provides an initial characterization of basic morphological properties of cultures of vascular smooth muscle cells (VSMC) from rat preglomerular resistance vessels and of the functional coupling of angiotensin II (ANG II) receptors to cytosolic free calcium concentration ([Ca2+]i (fura 2 fluorescence photometry). Fura-2 293-299 angiotensinogen Rattus norvegicus 225-231 9010006-3 1996 Using the Ca2+ indicator dye fura-2 in a cell culture model, we found that TNF-alpha (10-1000 U ml-1), but not interleukin 1 or 6, induced a slow but more than two-fold increase of the intracellular Ca2+ concentration, which could be blocked by Co2+ (1.0 mM), verapamil (100 microM) or omission of external Ca2+. Fura-2 29-35 tumor necrosis factor Homo sapiens 75-84 8952695-3 1996 AII also triggered a fast accumulation of IP3 and a rapid increase in [Ca2+]i in cells loaded with the Ca(2+)-responsive fluorescent dye fura-2. Fura-2 137-143 angiotensinogen Homo sapiens 0-3 8941710-2 1996 alpha3/beta4-nAChR-expressing clones were identified using the fura-2 Ca2+ imaging technique, and were further characterised by single-cell and whole-cell patch-clamp studies. Fura-2 63-69 tubulin beta 3 class III Homo sapiens 0-12 8908321-4 1996 Problems of mutual interference of Ca2+ and Cd2+ analysis with the fluorescent probe Fura-2 could be overcome by the use of the fluorine 19 nuclear magnetic resonance (19F-NMR) probe acetoxymethyl ester of 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N"N"-tetraacetic acid (5F-BAPTA), which allows the measurement of free intracellular Ca2+, Cd2+ and other metal ions concurrently. Fura-2 85-91 Cd2 molecule Rattus norvegicus 340-343 8917448-2 1996 Therefore, we analyzed bradykinin-activated Ca2+ influx into human foreskin fibroblast cells, HF-15, by fura-2 and 45Ca labeling to discriminate between Ca2+ influx into the fura-sensitive compartment and Ca uptake into fura-insensitive Ca stores. Fura-2 104-110 kininogen 1 Homo sapiens 23-33 8892650-5 1996 When fura-2-loaded cells were first exposed to RANTES, which failed to elevate the intracellular calcium concentration, the response to a subsequent challenge with eotaxin was inhibited in a dose-dependent manner. Fura-2 5-11 C-C motif chemokine 5 Cavia porcellus 47-53 8892650-5 1996 When fura-2-loaded cells were first exposed to RANTES, which failed to elevate the intracellular calcium concentration, the response to a subsequent challenge with eotaxin was inhibited in a dose-dependent manner. Fura-2 5-11 eotaxin Cavia porcellus 164-171 8914016-2 1996 The AVP-induced cellular signal transduction, including inositol 1,4,5-trisphosphate (IP3) production, fura-2 intracellular calcium measurements and cellular alkalinization, was significantly greater in cells of SHR than those of WKY. Fura-2 103-109 arginine vasopressin Rattus norvegicus 4-7 21594530-2 1996 Bradykinin stimulated an increase in [Ca2+](i), (monitored by the fura-2 fluorescence) in fibroblasts obtained from both the skin of a normal donor and the mesenter of a familial adenomatous polyposis (FAP) patient. Fura-2 66-72 kininogen 1 Homo sapiens 0-10 8874220-5 1996 In addition, fluorescence measurements with Fura-2-labeled eosinophils in the presence of EGTA indicated Ca(2+)-mobilization from intracellular stores by Eotaxin. Fura-2 44-50 C-C motif chemokine ligand 11 Homo sapiens 154-161 8913359-3 1996 By videomicroscopically examining Fura-2-loaded cells, we demonstrate that vasopressin induces a dose-dependent and receptor-mediated calcium influx fully inhibited by either 1 microM nifedipine or a pertussis toxin pretreatment and potentiated by 1 microM BAY K 8644. Fura-2 34-40 arginine vasopressin Rattus norvegicus 75-86 8917448-6 1996 In addition to the NO/cGMP-mediated Ca2+ influx into the fura-sensitive compartment, bradykinin enhanced 45Ca uptake into Ca stores that were not accessible to fura-2. Fura-2 160-166 kininogen 1 Homo sapiens 85-95 8831516-2 1996 In the present study, we report the first endothelial [Ca2+]i quantification by the fura 2 method in microscopically imaged venular capillaries of the isolated blood-perfused rat lung. Fura-2 84-90 carbonic anhydrase 2 Rattus norvegicus 55-58 8897698-7 1996 In Fura-2/AM loaded platelets, the rise of intracellular calcium level challenged by AA, collagen and thrombin were inhibited by C85. Fura-2 3-9 coagulation factor II, thrombin Homo sapiens 102-110 8939445-6 1996 TPEN, however, penetrates into synaptoneurosomes and efficiently displaces Cd2+ from the Fura-2-Cd2+ complex as shown by Fura-2 fluorescence recordings. Fura-2 89-95 Cd2 molecule Rattus norvegicus 75-78 8939445-6 1996 TPEN, however, penetrates into synaptoneurosomes and efficiently displaces Cd2+ from the Fura-2-Cd2+ complex as shown by Fura-2 fluorescence recordings. Fura-2 89-95 Cd2 molecule Rattus norvegicus 96-99 8795640-5 1996 Here, we report that NPY caused a long-term depression in both electrical activity and intracellular calcium levels of neurons, as studied with whole-cell patch-clamp recording and Fura-2 digital imaging. Fura-2 181-187 neuropeptide Y Homo sapiens 21-24 8831566-8 1996 These in vitro changes in fura-2 fluorescence, nevertheless, were small relative to changes in fura-2 fluorescence produced by elevation in intracellular Ca2+ concentrations in response to physiological stimulation of the cells (serum feeding after serum starvation). Fura-2 95-101 carbonic anhydrase 2 Mus musculus 154-157 8914860-5 1996 The cytosolic Ca2+ mobilization in response to the NK1 agonist was monitored both by spectrofluorimetric and single-cell image analysis determinations on adherent cells loaded with the Ca(2+)-sensitive fluorescent indicators Fura-2/AM and Indo-1, respectively. Fura-2 225-231 tachykinin receptor 1 Homo sapiens 51-54 8780286-2 1996 Using digital imaging microscopy with the Ca2+ indicator fura-2, the effects of halothane on the intracellular [Ca2+] dynamics induced by arginine vasopressin (AVP) in the perinuclear region and cytosol in single cultured smooth muscle cells of rat aorta were studied. Fura-2 57-63 arginine vasopressin Rattus norvegicus 147-158 8780286-2 1996 Using digital imaging microscopy with the Ca2+ indicator fura-2, the effects of halothane on the intracellular [Ca2+] dynamics induced by arginine vasopressin (AVP) in the perinuclear region and cytosol in single cultured smooth muscle cells of rat aorta were studied. Fura-2 57-63 arginine vasopressin Rattus norvegicus 160-163 8712453-4 1996 METHODS: The effect of halothane and isoflurane on the Ca2+ response to bradykinin of bovine aortic endothelial (BAE) cells was investigated using the fluorescent Ca2+ indicator fura-2. Fura-2 178-184 kininogen 1 Bos taurus 72-82 8892530-2 1996 Because these drugs are frequently used in combination, the present study was designed to investigate how they interact to modify the Ca2+ transient and associated contraction in fura2-loaded cardiomyocytes. Fura-2 179-184 carbonic anhydrase 2 Homo sapiens 134-137 8756565-3 1996 Video microscopy experiments performed on fura-2-loaded adrenal medullary or adrenal glomerulosa cell primary cultures showed that vasopressin dose dependently mobilized intracellular calcium, suggesting that functional vasopressin receptors are expressed in these tissues. Fura-2 42-48 arginine vasopressin Rattus norvegicus 131-142 8756565-3 1996 Video microscopy experiments performed on fura-2-loaded adrenal medullary or adrenal glomerulosa cell primary cultures showed that vasopressin dose dependently mobilized intracellular calcium, suggesting that functional vasopressin receptors are expressed in these tissues. Fura-2 42-48 arginine vasopressin Rattus norvegicus 220-231 8752105-2 1996 Bath perfusion or rapid puffer application of angiotensin II (Ang II) (1-100 nM) increased [Ca2+]i in both polygonal and stellate astroglia when measured using fura-2 imaging fluorescence microscopy. Fura-2 160-166 angiotensinogen Rattus norvegicus 46-60 8752105-2 1996 Bath perfusion or rapid puffer application of angiotensin II (Ang II) (1-100 nM) increased [Ca2+]i in both polygonal and stellate astroglia when measured using fura-2 imaging fluorescence microscopy. Fura-2 160-166 angiotensinogen Rattus norvegicus 62-68 8914862-3 1996 Functional coupling in hCCK-B.CHO cells was demonstrated using agonist stimulated mobilization of intracellular Ca2+, measured with the FURA-2 technique. Fura-2 136-142 cholecystokinin B receptor Homo sapiens 23-29 8760070-3 1996 PLB-deficient myocytes also demonstrated significant increases in the peak amplitude of the fura 2 fluorescence ratio and the rates of rising and falling phases of the Ca2+ transient. Fura-2 92-98 phospholamban Mus musculus 0-3 8753870-8 1996 Fura-2-based videoimaging showed that treatment with gp120 enhanced the ability of NMDA to increase neuronal [Ca2+]i. Fura-2 0-6 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 53-58 8864566-1 1996 Digital-imaging microscopy of Fura-2-loaded Chinese hamster ovary cells, stably expressing the cholecystokinin-A receptor, revealed that both the C-terminal octapeptide of cholecystokinin (CCKB) and its analogue JMV-180, which acts as an agonist at the high-affinity CCK-A receptor, recruited CHO-CCK-A cells dose-dependently in terms of receptor-mediated Ca2+ mobilization. Fura-2 30-36 cholecystokinin receptor type A Cricetulus griseus 95-121 8805305-5 1996 RESULTS: We detected subcellular Ca2+ signals during the response of single Fura2-loaded HeLa cells to histamine. Fura-2 76-81 carbonic anhydrase 2 Homo sapiens 33-36 8662890-7 1996 Of the chemokines tested, only murine MIP-1alpha, human and murine MIP-1beta and RANTES, human MCP-2, and JE were able to induce mobilization of intracellular Ca2+ from fura-2-loaded HEK 293 cells expressing the receptor. Fura-2 169-175 chemokine (C-C motif) ligand 3 Mus musculus 38-48 8662890-7 1996 Of the chemokines tested, only murine MIP-1alpha, human and murine MIP-1beta and RANTES, human MCP-2, and JE were able to induce mobilization of intracellular Ca2+ from fura-2-loaded HEK 293 cells expressing the receptor. Fura-2 169-175 chemokine (C-C motif) ligand 4 Mus musculus 67-76 8662890-7 1996 Of the chemokines tested, only murine MIP-1alpha, human and murine MIP-1beta and RANTES, human MCP-2, and JE were able to induce mobilization of intracellular Ca2+ from fura-2-loaded HEK 293 cells expressing the receptor. Fura-2 169-175 chemokine (C-C motif) ligand 5 Mus musculus 81-87 8662890-7 1996 Of the chemokines tested, only murine MIP-1alpha, human and murine MIP-1beta and RANTES, human MCP-2, and JE were able to induce mobilization of intracellular Ca2+ from fura-2-loaded HEK 293 cells expressing the receptor. Fura-2 169-175 C-C motif chemokine ligand 8 Homo sapiens 95-100 8782088-7 1996 When intracellular calcium mobilization was measured by Fura-2/AM cell labeling and spectrofluorometric monitoring, bombesin (100 nM) induced rapid calcium mobilization in both PC-3 (> 200% of baseline) and DU-145 (> 100% of baseline) cells, but not in LNCaP cells. Fura-2 56-62 gastrin releasing peptide Homo sapiens 116-124 8813647-1 1996 We have evaluated the application of the fura-2 method to detect cytosolic Ca2+ increase in gastric cells expressing CCKB/gastrin receptors, in order to screen gastrin receptor antagonists, as an alternative to functional studies. Fura-2 41-47 gastrin/cholecystokinin type B receptor Oryctolagus cuniculus 117-121 8813647-1 1996 We have evaluated the application of the fura-2 method to detect cytosolic Ca2+ increase in gastric cells expressing CCKB/gastrin receptors, in order to screen gastrin receptor antagonists, as an alternative to functional studies. Fura-2 41-47 gastrin Oryctolagus cuniculus 122-129 8762095-4 1996 Ca2+ release was measured by microfluorometry with Fura-2. Fura-2 51-57 carbonic anhydrase 2 Oryctolagus cuniculus 0-3 8967450-1 1996 In freshly isolated individual rat adipocytes, cytosolic free Ca2+ concentration ([Ca2+]i) as measured with fura 2 slowly declined during incubation but was sustained, or even somewhat increased, by brief treatment with growth hormone (GH) at the beginning of a 3-h incubation period. Fura-2 108-114 gonadotropin releasing hormone receptor Rattus norvegicus 220-234 8627363-4 1996 Fura-2 Ca2+ imaging showed that an increase in presynaptic cytosolic Ca2+ ([Ca2+]i) accompanied the synaptic potentiation by BDNF, whereas no change in [Ca2+]i was observed during synaptic potentiation by CNTF. Fura-2 0-6 LOC108713815 Xenopus laevis 125-129 8967451-0 1996 Growth hormone increases calcium uptake in rat fat cells by a mechanism dependent on protein kinase C. Growth hormone (GH; 500 ng/ml) rapidly doubled cytosolic free Ca2+ concentration ([Ca2+]i) in rat adipocytes as determined with the Ca2+ indicator fura 2. Fura-2 250-256 gonadotropin releasing hormone receptor Rattus norvegicus 0-14 8967451-0 1996 Growth hormone increases calcium uptake in rat fat cells by a mechanism dependent on protein kinase C. Growth hormone (GH; 500 ng/ml) rapidly doubled cytosolic free Ca2+ concentration ([Ca2+]i) in rat adipocytes as determined with the Ca2+ indicator fura 2. Fura-2 250-256 gonadotropin releasing hormone receptor Rattus norvegicus 103-117 8754275-0 1996 [Study of calcium-binding properties of the Mts-1 protein and its recombinant analog using the fluorescent probe Fura-2]. Fura-2 113-119 malignant transformation suppression-1 Homo sapiens 44-49 8754275-1 1996 A procedure based on the ability of Mts-1 to competitively inhibit the formation of Fura-2 complexes with Ca(2+)-ions is described. Fura-2 84-90 malignant transformation suppression-1 Homo sapiens 36-41 8761891-5 1996 Fura-2 was used to measure basal and angiotensin II-stimulated intracellular free calcium concentration. Fura-2 0-6 angiotensinogen Rattus norvegicus 37-51 8741218-4 1996 Parvalbumin-transfected and mock-transfected cells were loaded with the calcium indicator fura-2 and were exposed, in the same dish, to different concentrations of the calcium ionophore A23187 or to KCI. Fura-2 90-96 parvalbumin Homo sapiens 0-11 8611703-1 1996 Changes in intracellular calcium concentration [Ca2+]i of fura-2-loaded human platelet during its adhesion to a fibrinogen-coated surface were studied, using a flow chamber mounted on an epifluorescence microscope equipped with digital-ratio imaging. Fura-2 58-64 fibrinogen beta chain Homo sapiens 112-122 8641274-3 1996 When Fura 2 was used to report the luminal free [Ca2+] after inhibition of further Ca2+ uptake, submaximal concentrations of InsP3 caused rapid, partial decreases in fluorescence ratios. Fura-2 5-11 carbonic anhydrase 2 Homo sapiens 49-52 8641274-3 1996 When Fura 2 was used to report the luminal free [Ca2+] after inhibition of further Ca2+ uptake, submaximal concentrations of InsP3 caused rapid, partial decreases in fluorescence ratios. Fura-2 5-11 carbonic anhydrase 2 Homo sapiens 83-86 8641274-7 1996 In stores co-loaded with both indicators, InsP3-evoked Ca2+ release appeared quantal with Fura 2 and largely non-quantal with Calcium Green-5N; the discrepancy is not, therefore, a direct effect of the indicators. Fura-2 90-96 carbonic anhydrase 2 Homo sapiens 55-58 8625903-1 1996 As the GnRH-induced secretion of gonadotropins is critically dependent upon an increase in the intracellular calcium ion concentration ([Ca2+]i) and modulated by gonadal factors, the effects of gonadal steroids on the pattern of calcium mobilization in single gonadotrophs of the male rat were examined using the fluorescent Ca2+ indicator fura-2/AM. Fura-2 340-346 gonadotropin releasing hormone 1 Rattus norvegicus 7-11 8626395-1 1996 Regulation of the cytosolic free Ca2+ concentration by nerve growth factor was investigated in C6-2B glioma cells newly expressing the high affinity nerve growth factor receptor trkA, using Fura-2 fluorescence ratio imaging. Fura-2 190-196 nerve growth factor Mus musculus 55-74 8848347-5 1996 Fura-2-loaded washed platelets were stimulated with the agonists collagen (2 micrograms/mL) or thrombin (1.0 U/mL). Fura-2 0-6 coagulation factor II, thrombin Homo sapiens 95-103 8683457-5 1996 Application of 20 mM caffeine released Ca2+ from the sarcoplasmic reticulum (SR), resulting in a transient increase in the fura-2 fluorescence ratio. Fura-2 123-129 carbonic anhydrase 2 Rattus norvegicus 39-42 8603609-2 1996 We measured [Ca2+]i with fura-2 and recorded ion currents and membrane potentials with the whole cell configuration of the patch-clamp technique to elucidate the mechanism of PACAP-induced [Ca2+]i increase in bovine adrenal chromaffin cells. Fura-2 25-31 adenylate cyclase activating polypeptide 1 Bos taurus 175-180 8984102-1 1996 To clarify the relationship between cellular Ca2+ handling and salt sensitivity, we evaluated cytosolic free Ca2+ ([Ca2+]i) in fura-2 loaded platelets isolated from 20 inpatients with essential hypertension. Fura-2 127-133 carbonic anhydrase 2 Homo sapiens 109-112 8984102-1 1996 To clarify the relationship between cellular Ca2+ handling and salt sensitivity, we evaluated cytosolic free Ca2+ ([Ca2+]i) in fura-2 loaded platelets isolated from 20 inpatients with essential hypertension. Fura-2 127-133 carbonic anhydrase 2 Homo sapiens 109-112 8789126-1 1996 The effect of the fluorescent Ca2+ indicator dye Fura-2 on Ca2+ dynamics was studied in proximal apical dendrites of neocortical layer V and hippocampal CA1 pyramidal neurons in rat brain slices using somatic whole-cell recording and a charge-coupled device camera. Fura-2 49-55 carbonic anhydrase 2 Rattus norvegicus 59-62 8789126-1 1996 The effect of the fluorescent Ca2+ indicator dye Fura-2 on Ca2+ dynamics was studied in proximal apical dendrites of neocortical layer V and hippocampal CA1 pyramidal neurons in rat brain slices using somatic whole-cell recording and a charge-coupled device camera. Fura-2 49-55 carbonic anhydrase 1 Rattus norvegicus 153-156 8789126-2 1996 A single action potential evoked a transient increase of intradendritic calcium concentration ([Ca2+]i) that was reduced in size and prolonged when the Fura-2 concentration was increased from 20 to 250 microM. Fura-2 152-158 carbonic anhydrase 2 Rattus norvegicus 96-99 8815209-3 1996 For this purpose, the Ca2+ fraction of the total cation current (named "fractional Ca2+ current") was measured directly from the change in the Ca(2+)-sensitive fura-2 fluorescence at 380 nm excitation wavelength. Fura-2 160-166 carbonic anhydrase 2 Rattus norvegicus 22-25 8815209-3 1996 For this purpose, the Ca2+ fraction of the total cation current (named "fractional Ca2+ current") was measured directly from the change in the Ca(2+)-sensitive fura-2 fluorescence at 380 nm excitation wavelength. Fura-2 160-166 carbonic anhydrase 2 Rattus norvegicus 83-86 8593933-3 1996 [Ca2+]i in single rat pancreatic beta-cells was measured by dual-wavelength fura-2 microfluorometry. Fura-2 76-82 carbonic anhydrase 2 Rattus norvegicus 1-4 8611034-2 1996 In fura-2-loaded cells, AVP induced a rapid (0.5-2 min) transient increase in [Ca2+]i that was followed by a smaller sustained increase in [Ca2+]i. Fura-2 3-9 arginine vasopressin Rattus norvegicus 24-27 9075574-5 1996 Ristocetin-mediated binding of vWF to human platelets promoted a slow rise in Fura-2 fluorescence ratio. Fura-2 78-84 von Willebrand factor Homo sapiens 31-34 8821822-4 1996 Fura-2 fluorescence was measured in an inverted microscope at 37 degrees C. Angiotensin II (0.1 nmol/liter) and ATP (0.1 mumol/liter) induced a rapid transient increase of [Ca2+]i, which was followed by a sustained plateau (N = 37 and N = 24). Fura-2 0-6 angiotensinogen Rattus norvegicus 76-90 8717425-4 1996 Results from Fura-2 microfluorometry and Fluo-3 confocal microscopy suggest a potential cross talk between the increase of cytosolic free Ca2+ and the uptake and release of Ca2+ by the nucleus during spontaneous contraction of single myocytes. Fura-2 13-19 carbonic anhydrase 2 Gallus gallus 138-141 8717425-4 1996 Results from Fura-2 microfluorometry and Fluo-3 confocal microscopy suggest a potential cross talk between the increase of cytosolic free Ca2+ and the uptake and release of Ca2+ by the nucleus during spontaneous contraction of single myocytes. Fura-2 13-19 carbonic anhydrase 2 Gallus gallus 173-176 8573071-9 1996 Using fura 2-loaded platelets, the rise in the cytosolic free Ca2+ concentration induced by cathepsin G was shown to result, as for thrombin, from both mobilization of internal stores and Ca2+ entry across the plasma membrane. Fura-2 6-12 cathepsin G Homo sapiens 92-103 12013483-12 1996 To determine whether the depression of vascular contraction by these agents was correlated with cell calcium changes, the angiotensin II-induced calcium mobilization was determined in Fura-2 loaded cells, before and after treatment with these inhibitors. Fura-2 184-190 angiotensinogen Rattus norvegicus 122-136 8721612-4 1996 Further detection of a rise in the intracellular free Ca2+ concentration ([Ca2+]i) in cells exposed to THQ plus Ca2+, together with the partial protection conferred by the intracellular Ca(2+)-chelator fura-2 against cell growth inhibition, indicated that a disruption of Ca2+ homeostasis is a determinant event in THQ cytotoxicity. Fura-2 202-208 carbonic anhydrase 2 Cricetulus griseus 54-57 8721612-4 1996 Further detection of a rise in the intracellular free Ca2+ concentration ([Ca2+]i) in cells exposed to THQ plus Ca2+, together with the partial protection conferred by the intracellular Ca(2+)-chelator fura-2 against cell growth inhibition, indicated that a disruption of Ca2+ homeostasis is a determinant event in THQ cytotoxicity. Fura-2 202-208 carbonic anhydrase 2 Cricetulus griseus 75-78 8721612-4 1996 Further detection of a rise in the intracellular free Ca2+ concentration ([Ca2+]i) in cells exposed to THQ plus Ca2+, together with the partial protection conferred by the intracellular Ca(2+)-chelator fura-2 against cell growth inhibition, indicated that a disruption of Ca2+ homeostasis is a determinant event in THQ cytotoxicity. Fura-2 202-208 carbonic anhydrase 2 Cricetulus griseus 75-78 8721612-4 1996 Further detection of a rise in the intracellular free Ca2+ concentration ([Ca2+]i) in cells exposed to THQ plus Ca2+, together with the partial protection conferred by the intracellular Ca(2+)-chelator fura-2 against cell growth inhibition, indicated that a disruption of Ca2+ homeostasis is a determinant event in THQ cytotoxicity. Fura-2 202-208 carbonic anhydrase 2 Cricetulus griseus 75-78 8871886-2 1996 AVP (ED50, 10(-7) mol/l) and oxytocin (ED50, 3 x 10(-8) mol/l) induced a rapid, transient and dose-dependent increase in [Ca2+]i as detected by fura-2 microfluorimetry. Fura-2 144-150 arginine vasopressin Homo sapiens 0-3 7488000-2 1995 Fura-2 fluorometry revealed that ADM decreases both the cytosolic Ca2+ concentration ([Ca2+]i) and tension induced by 0.3 microM phenylephrine (PE) in porcine RASMCs. Fura-2 0-6 adrenomedullin Homo sapiens 33-36 8961380-4 1996 Substance P (30 microM) also induced a transient increase in the intracellular Ca2+ concentration ([Ca2+]i) in both synovial A and B cells as measured by a Ca2+ indicator, fura 2, BAPTA-AM and an inositol-1,4-5-triphosphate (IP3) receptor antagonist, heparin, inhibited the substance P induced increase in [Ca2+]i, but they had no effects on oxyradical production. Fura-2 172-178 tachykinin precursor 1 Homo sapiens 0-11 8822433-5 1995 The SABP also induces an increase in intracellular Ca2+ in spermatozoa, as seen by FURA-2 AM studies. Fura-2 83-89 prolactin induced protein Homo sapiens 4-8 8848007-4 1995 Pharmacological and functional analyses carried out on Fura-2-loaded cells showed that Ca2+ influx elicited by both growth factors is the summation of two distinct pathways, with the major pathway dependent on and the minor pathway independent of store depletion. Fura-2 55-61 carbonic anhydrase 2 Homo sapiens 87-90 9275708-1 1996 Using fluorescent probe Fura-2/AM, ginsenoside Rb1 (10, 50 and 100 mumol.L-1) was shown to dose-dependently reduce intracellular Ca2+ concentration of freshly dissociated brain cells isolated from newborn rats. Fura-2 24-30 RB transcriptional corepressor 1 Rattus norvegicus 47-50 8934635-4 1995 Ricin pretreatment nearly doubled the intracellular-free Ca2+ concentration as measured by fura-2 fluorescence microscopy in isolated myocytes (p = 0.002). Fura-2 91-97 ricin Ricinus communis 0-5 8719876-5 1995 With single cell measurements of fura-2 loaded cells we demonstrate that SP-A raises the intracellular free calcium ion concentration 6 to 8 seconds after addition. Fura-2 33-39 surfactant protein A1 Rattus norvegicus 73-77 7554143-7 1995 Furthermore, the thrombin-induced rise in cytoplasmic [Ca2+]i was strongly attenuated by the cGMP-PK activator in fura 2-loaded aorta ECs. Fura-2 114-120 coagulation factor II, thrombin Homo sapiens 17-25 7568127-7 1995 The [Ca2+]i response to glutamate (quantified by fura-2 imaging) was markedly potentiated in neurons exposed to A beta, and this action of A beta was suppressed in cultures pretreated with TNFs. Fura-2 49-55 amyloid beta precursor protein Homo sapiens 112-118 8589290-6 1995 Fura-2 fluorescence ratio was not significantly changed with angiotensin II, Ap3A, or Ap4A but increased with vasopressin, ATP, Ap5A, and Ap6A by 0.08 +/- 0.02 (N = 13), 0.04 +/- 0.02 (N = 13), 0.03 +/- 0.01 (N = 14), and 0.03 +/- 0.01 (N = 10), respectively. Fura-2 0-6 arginine vasopressin Rattus norvegicus 110-121 7485450-5 1995 In fura 2-loaded proximal tubule cells, EGF caused a concentration-dependent increase in cytosolic Ca2+ concentration ([Ca2+]i), due to Ca2+ influx, which was inhibited by either ketoconazole or SKF-525A but not by indomethacin. Fura-2 3-9 pro-epidermal growth factor Oryctolagus cuniculus 40-43 7568127-7 1995 The [Ca2+]i response to glutamate (quantified by fura-2 imaging) was markedly potentiated in neurons exposed to A beta, and this action of A beta was suppressed in cultures pretreated with TNFs. Fura-2 49-55 amyloid beta precursor protein Homo sapiens 139-145 8519599-3 1995 Exposure of myo-[2-3H]-inositol prelabelled fibroblasts to HDL3 or LDL elicited major increases in IP1 and minor increases in IP2 and IP3 within 30 s. In fura-2 loaded suspended fibroblasts, HDL3 and LDL increased intracellular Ca2+ concentrations ([Ca2+]i) with comparable rapid, transient kinetics. Fura-2 154-160 HDL3 Homo sapiens 59-63 8519599-3 1995 Exposure of myo-[2-3H]-inositol prelabelled fibroblasts to HDL3 or LDL elicited major increases in IP1 and minor increases in IP2 and IP3 within 30 s. In fura-2 loaded suspended fibroblasts, HDL3 and LDL increased intracellular Ca2+ concentrations ([Ca2+]i) with comparable rapid, transient kinetics. Fura-2 154-160 HDL3 Homo sapiens 191-195 7550072-1 1995 In fura-2-loaded human periodontal ligament (HPDL) cells, bradykinin induced a rapidly transient increase and subsequently sustained increase in cytosolic Ca2+ ([Ca2+]i). Fura-2 3-9 kininogen 1 Homo sapiens 58-68 9816080-5 1995 In fura-2-loaded ovarian carcinoma cells, ET-1 induced dose-dependent increases in cytoplasmic Ca2+ concentration. Fura-2 3-9 endothelin 1 Homo sapiens 42-46 7501705-10 1995 (4) Using FURA-2 to monitor intracellular free calcium simultaneous with contractile tension, we demonstrated that the alterations in the contractile responses observed at different concentrations of extracellular Mg2+ correlated with similar changes in intracellular free calcium. Fura-2 10-16 mucin 7, secreted Homo sapiens 214-217 7582546-2 1995 The contribution of sarco-endoplasmic reticulum Ca(2+)-ATPases (SERCA)-regulated Ca2+ stores to the increase in intracellular free calcium ([Ca2+]i) induced by bradykinin (BK) was investigated in fura-2 loaded human tracheal smooth muscle cells (TSMC). Fura-2 196-202 kininogen 1 Homo sapiens 160-170 7478924-9 1995 A half-maximal initial increase in the fura-2 fluorescence ratio was reached at 22 nmol/l, 0.9 micromol/l, 2.0 micromol/l and 4.0 micromol/l with Ang II, Ap3A, ATP and Ap4A, respectively. Fura-2 39-45 angiotensinogen Rattus norvegicus 146-152 8564193-2 1995 In the present study, we determined whether ETA receptors are present on endothelial cells in situ, by use of front-surface fluorometry of fura-2-loaded porcine aortic valvular strips and reverse transcription polymerase chain reaction (RT-PCR). Fura-2 139-145 endothelin receptor type A Sus scrofa 44-47 8537818-6 1995 The value of beta, the buffering power of myoplasm, was determined in fibers equilibrated with a combination of EGTA, phenol red, and fura-2; its mean value was 22 mM/pH unit. Fura-2 134-140 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 13-17 7585889-1 1995 The effect of an induction of transforming Ha-ras on Ca2+ influx into NIH3T3 cells was studied employing Fura-2 quenching by Mn2+. Fura-2 105-111 Harvey rat sarcoma virus oncogene Mus musculus 43-49 7585894-2 1995 ATP induced an increase in [Ca2+]i of these epithelial cells loaded with Fura-2 in a dose-dependent manner (1-100 microM). Fura-2 73-79 carbonic anhydrase 2 Homo sapiens 28-31 7632163-10 1995 The two fenamates inhibited the fMLP-induced increase in intracellular free calcium in fura-2 loaded PMNs in the presence but not in the absence of extracellular calcium. Fura-2 87-93 formyl peptide receptor 1 Homo sapiens 32-36 7631736-2 1995 Digitized image analysis of fura 2-loaded human embryonic lung fibroblasts indicated specific transient increases in [Ca2+]i beginning in some cells within the first 5 min of incubation with cross-linked MAb2 (70-750 nM, P < 0.01), which were similar in timing and intracellular distribution to those induced by HCMV. Fura-2 28-34 carbonic anhydrase 2 Homo sapiens 118-121 7750471-5 1995 In fura 2-loaded VSMC, 5-min incubation with PTH or PTHrP attenuated angiotensin II (Ang II)-induced calcium mobilization, an effect that was reduced by preincubation of VSMC with PTH for 1.5 h. Similarly, 1.5-h preincubation with PTH or PTHrP decreased the cAMP response to these peptides but not to forskolin or NaF. Fura-2 3-9 parathyroid hormone Bos taurus 45-48 7635245-3 1995 In human platelets loaded with fura-2, the intracellular rise of [Ca2+] induced by thrombin was dose-dependently inhibited by MM-706 with an approximate IC50 of 100 microM. Fura-2 31-37 coagulation factor II, thrombin Homo sapiens 83-91 7603033-6 1995 The change in [Ca2+]i in thymocytes after TPEN treatment was monitored using the fluorescence Ca2+ indicator dye, fura-2. Fura-2 114-120 carbonic anhydrase 2 Homo sapiens 15-18 7539422-6 1995 Calcium-permeable cation channel activity was detected in CD20-expressing cells by whole cell patch clamp recording and microfluorometric determination of the cytoplasmic Ca2+ concentration using fura-2. Fura-2 196-202 membrane-spanning 4-domains, subfamily A, member 1 Mus musculus 58-62 7750471-5 1995 In fura 2-loaded VSMC, 5-min incubation with PTH or PTHrP attenuated angiotensin II (Ang II)-induced calcium mobilization, an effect that was reduced by preincubation of VSMC with PTH for 1.5 h. Similarly, 1.5-h preincubation with PTH or PTHrP decreased the cAMP response to these peptides but not to forskolin or NaF. Fura-2 3-9 parathyroid hormone like hormone Bos taurus 52-57 7750471-5 1995 In fura 2-loaded VSMC, 5-min incubation with PTH or PTHrP attenuated angiotensin II (Ang II)-induced calcium mobilization, an effect that was reduced by preincubation of VSMC with PTH for 1.5 h. Similarly, 1.5-h preincubation with PTH or PTHrP decreased the cAMP response to these peptides but not to forskolin or NaF. Fura-2 3-9 parathyroid hormone Bos taurus 52-55 7750471-5 1995 In fura 2-loaded VSMC, 5-min incubation with PTH or PTHrP attenuated angiotensin II (Ang II)-induced calcium mobilization, an effect that was reduced by preincubation of VSMC with PTH for 1.5 h. Similarly, 1.5-h preincubation with PTH or PTHrP decreased the cAMP response to these peptides but not to forskolin or NaF. Fura-2 3-9 parathyroid hormone Bos taurus 52-55 7744827-3 1995 Occupation of either receptor species, with 100 nM peptide YY (PYY) or 10 microM phenylephrine (PE), respectively, resulted in a rapid increase in the cytoplasmic free calcium concentration ([Ca2+]i) as assessed with Fura-2/AM. Fura-2 217-223 peptide YY Cricetulus griseus 51-61 7666368-2 1995 Arg8-vasopressin (AVP)-regulated Ca2+ transport were investigated in fura-2-loaded A7r5 cells using both single cell and population measurements. Fura-2 69-75 arginine vasopressin Rattus norvegicus 0-16 7666368-2 1995 Arg8-vasopressin (AVP)-regulated Ca2+ transport were investigated in fura-2-loaded A7r5 cells using both single cell and population measurements. Fura-2 69-75 arginine vasopressin Rattus norvegicus 18-21 7539257-2 1995 In the present study we show that in Fura 2 loaded GH4C1 cells, caffeine inhibited, in a dose-dependent manner, the Ca2+ response induced by a submaximally effective dose (3 nM) of thyrotropin-releasing hormone (TRH). Fura-2 37-43 thyrotropin releasing hormone Rattus norvegicus 181-210 7744827-3 1995 Occupation of either receptor species, with 100 nM peptide YY (PYY) or 10 microM phenylephrine (PE), respectively, resulted in a rapid increase in the cytoplasmic free calcium concentration ([Ca2+]i) as assessed with Fura-2/AM. Fura-2 217-223 peptide YY Cricetulus griseus 63-66 7729011-4 1995 In cultured bovine aortic endothelial cells, extracellular application of bradykinin (BK, 10 to 20 mumol/L) caused transient (sometimes oscillatory) increase in [Ca2+]i, which was measured with the fluorescent Ca2+ indicator fura 2 and fluorescence imaging microscopy. Fura-2 225-231 kininogen 1 Bos taurus 74-84 7729011-4 1995 In cultured bovine aortic endothelial cells, extracellular application of bradykinin (BK, 10 to 20 mumol/L) caused transient (sometimes oscillatory) increase in [Ca2+]i, which was measured with the fluorescent Ca2+ indicator fura 2 and fluorescence imaging microscopy. Fura-2 225-231 kininogen 1 Bos taurus 86-88 7758834-3 1995 In the present study, the AVP-induced changes in cytosolic free Ca2+ ([Ca2+]i) were investigated in single fura-2 loaded insulin-secreting HIT cells. Fura-2 107-113 insulin Homo sapiens 121-128 7623276-13 1995 Measurements of free intracellular calcium concentration ([Ca2+]i) with the dye fura-2 showed that bFGF elicited sustained increases in [Ca2+]i that were completely dependent on external calcium and on the presence of the agonist and could last more than 1 h. 5. Fura-2 80-86 fibroblast growth factor 2 Mus musculus 99-103 7606345-10 1995 Both the contractions induced directly by caffeine and those produced following caffeine wash-out after ryanodine treatment were accompanied by a maintained increase in intracellular Ca2+ concentration measured with fura-2. Fura-2 216-222 carbonic anhydrase 2 Oryctolagus cuniculus 183-186 7895328-6 1995 In fura 2-loaded endothelial cells, inhibition of tyrosine kinases attenuated Ca2+ signaling after stimulation with either bradykinin (30 nmol/L) or thapsigargin (30 nmol/L). Fura-2 3-9 kininogen 1 Homo sapiens 123-133 7535010-3 1995 With the use of the perforated patch technique and fura 2 microfluorimetry, a fivefold greater increase in [Ca2+]i per unit decrease in membrane potential was obtained in the presence of SP (10 nM) compared with resting state, implying that SP increased Ca2+ conductance. Fura-2 51-57 tachykinin precursor 1 Homo sapiens 187-189 7772573-3 1995 In this study, BK-induced Ca2+ responses were examined in primary cultured human keratinocytes by video imaging fluorescence microscopy using fura-2. Fura-2 142-148 kininogen 1 Homo sapiens 15-17 7700249-1 1995 Measurement of the intracellular Ca2+ concentration ([Ca2+]i) in fura-2-loaded single cells of the human neuroblastoma line SH-SY5Y indicated coexpression of muscarinic and bradykinin receptors linked to activation of phosphoinositidase C (PIC). Fura-2 65-71 kininogen 1 Homo sapiens 173-183 7700249-1 1995 Measurement of the intracellular Ca2+ concentration ([Ca2+]i) in fura-2-loaded single cells of the human neuroblastoma line SH-SY5Y indicated coexpression of muscarinic and bradykinin receptors linked to activation of phosphoinositidase C (PIC). Fura-2 65-71 phospholipase C beta 1 Homo sapiens 218-238 7700249-1 1995 Measurement of the intracellular Ca2+ concentration ([Ca2+]i) in fura-2-loaded single cells of the human neuroblastoma line SH-SY5Y indicated coexpression of muscarinic and bradykinin receptors linked to activation of phosphoinositidase C (PIC). Fura-2 65-71 phospholipase C beta 1 Homo sapiens 240-243 7626323-2 1995 EGF significantly increased [Ca++]i measured in fura-2-loaded, individual cells. Fura-2 48-54 epidermal growth factor like 1 Rattus norvegicus 0-3 7626327-2 1995 Cells loaded with the Ca2+ dye FURA-2 dose-dependently responded to CGRP (1-100 nM) with transient two-fold increases in [Ca2+]i. Fura-2 31-37 calcitonin-related polypeptide alpha Rattus norvegicus 68-72 7603843-1 1995 Ca2+ channel activities were recorded in the limb bud of embryonic day 4 chick with Ca2+ sensitive fluorescence (Fura-2) measurements and patch clamp techniques. Fura-2 113-119 carbonic anhydrase 2 Gallus gallus 0-3 7876236-4 1995 We have studied possible intracellular signaling effects of the FVIIa-TF interaction by measuring cytosolic free Ca2+ in single fura-2-loaded cells and found that 200 nM FVIIa caused Ca2+ transients in about 30% of human umbilical vein endothelial cells treated with interleukin-1 beta to express TF, compared to below 5% in uninduced cells. Fura-2 128-134 coagulation factor III, tissue factor Homo sapiens 70-72 7756530-4 1995 k21 represents the second-order rate constant of binding of Ca2+ and Fura-2 in the excited state, whereas k12 is the first-order rate constant of dissociation of the excited Ca2+:Fura-2 complex. Fura-2 179-185 keratin 12 Homo sapiens 106-109 7861766-6 1995 CCK octapeptide caused a specific increase in [Ca2+]i measured by Fura-2 fluorometry in H-PBMC and human T helper lymphocytes. Fura-2 66-72 cholecystokinin Homo sapiens 0-3 7859285-4 1995 Dextrans labeled with fluorescent Ca2+ indicators (calcium-Green 1 and Fura 2) are distributed uniformly in the nucleoplasm and can be used to show that changes in the external Ca2+ concentration produce rapid changes in the nucleoplasmic Ca2+ concentration. Fura-2 71-77 carbonic anhydrase 2 Homo sapiens 34-37 7859285-4 1995 Dextrans labeled with fluorescent Ca2+ indicators (calcium-Green 1 and Fura 2) are distributed uniformly in the nucleoplasm and can be used to show that changes in the external Ca2+ concentration produce rapid changes in the nucleoplasmic Ca2+ concentration. Fura-2 71-77 carbonic anhydrase 2 Homo sapiens 177-180 7859285-4 1995 Dextrans labeled with fluorescent Ca2+ indicators (calcium-Green 1 and Fura 2) are distributed uniformly in the nucleoplasm and can be used to show that changes in the external Ca2+ concentration produce rapid changes in the nucleoplasmic Ca2+ concentration. Fura-2 71-77 carbonic anhydrase 2 Homo sapiens 177-180 7867789-1 1995 The potency of annexin V to transport Ca2+ ions across phospholipid membranes was investigated, using large unilamellar phospholipid vesicles loaded with the Ca2+ indicator fura-2. Fura-2 173-179 annexin A5 Homo sapiens 15-24 7867789-3 1995 However, if the vesicles were first incubated with annexin V in the absence of Ca2+, subsequent addition of Ca2+ produced a fluorescence signal due to binding of Ca2+ to fura-2. Fura-2 170-176 annexin A5 Homo sapiens 51-60 7867789-5 1995 Our results show that annexin V causes loss of vesicle integrity in the absence of Ca2+, and leakage of trapped fura-2, rather than inward Ca2+ transport. Fura-2 112-118 annexin A5 Homo sapiens 22-31 7740516-1 1995 Treatment of Fura-2 loaded HEL cells, a human megakaryocyte-like cell line, with P2-purinoceptor nucleotide ligands (ADP, ATP, UTP, 2-methylthio-ATP) evoked a rise in cytosolic calcium. Fura-2 13-19 pyrimidinergic receptor P2Y6 Homo sapiens 81-96 7550580-4 1995 PTH also increases intracellular calcium as measured by the technique using Fura-2 fluorescent dye and stimulates phosphoinositide degradation as evaluated by column chromatography. Fura-2 76-82 parathyroid hormone Homo sapiens 0-3 7536247-4 1995 Several P450 inhibitors diminished the sustained [Ca2+]i plateau response to agonist or intracellular Ca2+ store depletion with ATPase inhibitors by 31-69% (fura-2 technique). Fura-2 157-163 dynein axonemal heavy chain 8 Homo sapiens 128-134 7530414-4 1995 The increase in intracellular calcium [Ca2+]in evoked by substance P was measured by the fluorescent indicator fura-2 loaded in a murine cell line expressing substance P (NK1) receptors. Fura-2 111-117 tachykinin 1 Mus musculus 57-68 7530414-4 1995 The increase in intracellular calcium [Ca2+]in evoked by substance P was measured by the fluorescent indicator fura-2 loaded in a murine cell line expressing substance P (NK1) receptors. Fura-2 111-117 tachykinin 1 Mus musculus 158-169 7623611-1 1995 Total gangliosides from bovine brain at micromolar concentration induce intracellular Ca2+ increments in a temperature, time and dose dependent manner when assayed with suspensions of rat macrophages, rat and chicken neurons, human erythrocytes and liposomes, loaded with the fluorescent Ca2+ indicator FURA 2. Fura-2 303-309 carbonic anhydrase 2 Bos taurus 86-89 8001384-5 1995 MEASUREMENTS AND MAIN RESULTS: Endothelin-1 increased the intracellular calcium concentration in fura-2 loaded human monocytes to a mean value of 37 +/- 4 nmol. Fura-2 97-103 endothelin 1 Homo sapiens 31-43 7529236-3 1995 We report that addition of vasopressin to fura-2-loaded glomerular mesangial cells in culture markedly accelerated the rate at which Mn2+ quenched fura-2 fluorescence at its Ca(2+)-insensitive wavelength in the presence of extracellular NaCl, but that this quench response was attenuated when Cl- was removed from the extracellular medium by equimolar substitution with impermeant anions (gluconate, methanesulfonate, acetate, lactate). Fura-2 42-48 arginine vasopressin Homo sapiens 27-38 7529236-3 1995 We report that addition of vasopressin to fura-2-loaded glomerular mesangial cells in culture markedly accelerated the rate at which Mn2+ quenched fura-2 fluorescence at its Ca(2+)-insensitive wavelength in the presence of extracellular NaCl, but that this quench response was attenuated when Cl- was removed from the extracellular medium by equimolar substitution with impermeant anions (gluconate, methanesulfonate, acetate, lactate). Fura-2 147-153 arginine vasopressin Homo sapiens 27-38 7529236-5 1995 Addition of the Cl- channel inhibitor, 5-nitro-2-(3-phenylpropylaminobenzoic acid) (NPPB), also inhibited Mn(2+)-induced quench of fura-2 fluorescence following vasopressin addition. Fura-2 131-137 arginine vasopressin Homo sapiens 161-172 7730791-4 1995 In dark-adapted photoreceptors, the initial Cai was 0.40 +/- 0.22 microM (SD, n = 7) as measured with fura-2. Fura-2 102-108 carbonic anhydrase 1 Homo sapiens 44-47 7705470-1 1994 We examined the mechanism of endothelin-1-induced contraction in the porcine coronary artery by assessing the changes in myosin light chain phosphorylation and cytosolic Ca2+ concentration ([Ca2+]i) determined by fura-2 and front-surface fluorometry. Fura-2 213-219 endothelin 1 Homo sapiens 29-41 7565458-4 1995 Using video image analysis of single cells loaded with fura-2, PTH (10-(7) M) induced transient increases in [Ca2+]i preferentially in cells in S phase, with 82% response frequency whereas cells in G1 phase responded poorly to PTH with only 10% response frequency. Fura-2 55-61 parathyroid hormone Rattus norvegicus 63-66 7479311-8 1995 In intact cells, angiotensin II caused a rapid increase in intracellular calcium (Ca2+) using Fura-2 as a Ca2+ indicator. Fura-2 94-100 angiotensinogen Rattus norvegicus 17-31 7962102-10 1994 Using fura-2 imaging of single cells, we show that soluble N-cadherin induces Ca2+ increases in CG neuron cell bodies, and, importantly, in growth cones. Fura-2 6-12 cadherin 2 Gallus gallus 59-69 7964729-1 1994 Fura-2 digital imaging microfluorimetry was used to evaluate the Ca2+ signals generated in single clonal human neuroepithelioma cells (SK-N-MCIXC) in response to agonists that stimulate phosphoinositide hydrolysis. Fura-2 0-6 hedgehog acyltransferase Homo sapiens 135-139 7898400-7 1994 Measurements of intracellular calcium activity ([Ca2+]i) with the indicator dye fura-2 revealed a sharp increase during glutamate exposure. Fura-2 80-86 carbonic anhydrase 2 Rattus norvegicus 49-52 7996447-2 1994 In the endothelium loaded with fura-2, ET-1 (1-100 nM) induced large transient increase followed by small sustained increase in cytosolic Ca++ level ([Ca++]i) in a concentration-dependent manner. Fura-2 31-37 endothelin-1 Oryctolagus cuniculus 39-43 7996199-2 1994 Using fura-2-based imaging we found that Ca2+ transients evoked by depolarization with 50 mM KCl were suppressed by the mu-opioid receptor agonist D-Ala2,N-MePhe4,Gly5-ol-enkephalin (DAMGO) and less effectively by the kappa-receptor agonist U-69,593. Fura-2 6-12 proenkephalin Rattus norvegicus 171-181 7767879-2 1994 ATPase activity was measured using a phosphoenolpyruvate-pyruvate kinase regenerating system for ATP that was monitored by NADH fluorescence changes, and Ca2+ was measured using fura 2 fluorescence. Fura-2 178-184 dynein axonemal heavy chain 8 Homo sapiens 0-6 7523388-4 1994 We also analyzed the kinetics of activation of intracellular IP3 receptor/Ca2+ channels by monitoring the quench of sequestered dye by the entry of cytoplasmic Mn2+ into fura-2-loaded intracellular IP3-sensitive organelles. Fura-2 170-176 inositol 1,4,5-trisphosphate receptor, type 3 Rattus norvegicus 61-73 8083757-2 1994 After selectively loading presynaptic structures in area CA1 with the calcium indicator fura-2, we simultaneously recorded a presynaptic calcium transient ([Ca]t) and the corresponding field excitatory postsynaptic potential (fEPSP) evoked by a single stimulus given to the Schaffer collateral-commissural (SCC) pathway. Fura-2 88-94 carbonic anhydrase 1 Homo sapiens 57-60 7530208-1 1994 The nature of the senktide response of the human NK3 receptor expressed in Chinese hamster ovary cells was characterised using the Ca2+ sensitive dye Fura-2 and imaging methods. Fura-2 150-156 tachykinin receptor 3 Homo sapiens 49-61 7523185-2 1994 Fura-2 measurements revealed that the RYR in T-cells functions as a ryanodine-sensitive, caffeine-insensitive Ca2+ release channel. Fura-2 0-6 ryanodine receptor 1 Homo sapiens 38-41 7520361-4 1994 Indeed, in fura-2-loaded cells, PAF stimulates [Ca2+]i increase with a median effective concentration of 5.6 nM. Fura-2 11-17 PCNA clamp associated factor Homo sapiens 32-35 7914703-1 1994 Fura-2 and imaging technology were used to detect intracellular Ca2+ changes in CA1 pyramidal cells in hippocampal slices. Fura-2 0-6 carbonic anhydrase 2 Homo sapiens 64-67 7830331-1 1994 We measured [Ca2+]i of guinea pig and rat myocytes with Ca2+ waves, using fura-2 fluorescence image processing. Fura-2 74-80 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 13-16 7914703-1 1994 Fura-2 and imaging technology were used to detect intracellular Ca2+ changes in CA1 pyramidal cells in hippocampal slices. Fura-2 0-6 carbonic anhydrase 1 Homo sapiens 80-83 7916897-8 1994 In MCF-7 and PC3 cells, we measured the [Ca2+]i using Fura-2 fluorescence and observed a decrease in MCF-7 cells from 126 to 99 nM when the cells were exposed for 6 days to 45 microM suramin. Fura-2 54-60 chromobox 8 Homo sapiens 13-16 8068023-1 1994 In single Fura-2 ester-loaded hepatocytes, stimulation by vasopressin, but not emptying of the agonist-sensitive Ca2+ store by 2,5-di-(t-butyl)hydroquinone, resulted in an increase in the rate of Fura-2 fluorescence-quenching by Mn2+. Fura-2 10-16 arginine vasopressin Homo sapiens 58-69 8068023-2 1994 Similarly, in cells microinjected with Fura-2 salt, vasopressin stimulated Mn2+ entry while 2,5-di-(t-butyl)hydroquinone or thapsigargin did not. Fura-2 39-45 arginine vasopressin Rattus norvegicus 52-63 7519011-11 1994 Both fMLP and thapsigargin stimulated quenching of Fura-2 fluorescence in the presence of extracellular Mn2+, whereas maitotoxin produced no Mn2+ quenching. Fura-2 51-57 formyl peptide receptor 1 Homo sapiens 5-9 7982265-1 1994 Bradykinin (BDK)-induced increases in intracellular Ca2+ concentration ([Ca2+]i) were monitored in cultured canine tracheal smooth muscle cells (TSMCs) using a fluorescent Ca2+ indicator, Fura-2. Fura-2 188-194 kininogen 1 Canis lupus familiaris 12-15 7519157-8 1994 Further, LIF treatment blocked the normal muscarinic receptor-mediated increase in intracellular calcium (fura-2 imaging), indicating a functional change in receptor phenotype. Fura-2 106-112 LIF, interleukin 6 family cytokine Rattus norvegicus 9-12 7953671-4 1994 Measurements of [Ca2+]i using fura-2 imaging revealed that the elevation of [Ca2+]i that occurred in response to long-term exposure to A beta was attenuated in neurons treated with staurosporine and K-252 compounds. Fura-2 30-36 amyloid beta precursor protein Rattus norvegicus 135-141 8026022-3 1994 METHODS AND RESULTS: Insulin (40 microU/mL) attenuated the 5-hydroxytryptamine (5-HT, serotonin; 10(-5) mol/L)-induced [Ca2+]i transient (measured by fura 2 fluorescence) in primary confluent canine femoral artery VSMCs in the presence of extracellular Ca2+. Fura-2 150-156 insulin Canis lupus familiaris 21-28 7944616-2 1994 METHODS: The release into the cytosol of Ca2+ from stores within individual neutrophils was determined using ratiometric imaging of fura2. Fura-2 132-137 carbonic anhydrase 2 Homo sapiens 41-44 8026022-6 1994 In support of those conclusions, we found that insulin inhibited the 5-HT-induced component of Mn2+ (a Ca2+ surrogate) influx (measured by fura 2 fluorescence quenching at the Ca2+ isosbestic excitation wavelength). Fura-2 139-145 insulin Canis lupus familiaris 47-54 7932382-3 1994 The presence of functional receptors was shown by inositol trisphosphate accumulation and a rise in the concentration of intracellular free calcium evoked by platelet-activating factor in myo-[2-3H]inositol-labelled and fura-2-loaded cells, respectively. Fura-2 220-226 PCNA clamp associated factor Homo sapiens 158-184 7960031-3 1994 Using the microspectrofluorimetric technique with fura-2, as calcium indicator, brief applications of OT on single astrocytes induced a transient and reversible dose-dependent increase of intracellular calcium concentration ([Ca2+]i) in most of the cells tested. Fura-2 50-56 oxytocin/neurophysin I prepropeptide Homo sapiens 102-104 8021298-6 1994 Pretreatment of Fura 2 loaded cells with 4-bromophenacyl bromide, an inhibitor of PLA2 activity, decreased the thapsigargin-induced entry of calcium, suggesting a role for PLA2 in the regulation of calcium entry. Fura-2 16-22 phospholipase A2 group IB Rattus norvegicus 82-86 8021298-6 1994 Pretreatment of Fura 2 loaded cells with 4-bromophenacyl bromide, an inhibitor of PLA2 activity, decreased the thapsigargin-induced entry of calcium, suggesting a role for PLA2 in the regulation of calcium entry. Fura-2 16-22 phospholipase A2 group IB Rattus norvegicus 172-176 8069676-1 1994 Cd2+ and Co2+ at micromolar concentrations increased the cytosolic free Ca2+ concentration, which was measured by fura-2 microfluorometry, in cat adrenal chromaffin cells. Fura-2 114-120 CD2 molecule Homo sapiens 0-3 8082131-1 1994 Calcium release in response to the activation of muscarinic M1 and histamine H1 receptors was studied in single N1E-115 cells using Fura-2 imaging. Fura-2 132-138 cholinergic receptor, muscarinic 1, CNS Mus musculus 60-89 8206566-4 1994 Their manual and ambulatory automated 24-hour blood pressure measurements were correlated with cytosolic calcium in resting and stimulated (thrombin-treated) fura 2-loaded platelets. Fura-2 158-164 coagulation factor II, thrombin Homo sapiens 140-148 8072857-1 1994 Ca(2+)-mediated Ca2+ spikes were analysed in fura-2-loaded megakaryocytes. Fura-2 45-51 carbonic anhydrase 2 Homo sapiens 16-19 7909692-1 1994 Acetoxymethyl ester (AM) derivatives of various fluorescent indicators (fura-2, fluo-3, indo-1, BCECF, calcein) are actively extruded by the multidrug transporter (MDR1, P-glycoprotein-Homolya, L. et al. Fura-2 72-78 ATP binding cassette subfamily B member 1 Homo sapiens 164-168 8144609-4 1994 After loading the cytosol of intact hepatocytes with Mn2+, both submaximal and maximal vasopressin doses caused complete quench of the entire intracellular pool of compartmentalized fura-2. Fura-2 182-188 arginine vasopressin Homo sapiens 87-98 8164648-5 1994 Third, although the changes in intracellular Ca2+ of fura-2/AM-preloaded human neutrophils elicited by N51/KC and IL-8 are similar, pretreatment of the cells with N51/KC did not result in a loss of response to a subsequent treatment with IL-8; in contrast, treatment with IL-8 did result in the subsequent desensitization to N51/KC. Fura-2 53-59 C-X-C motif chemokine ligand 8 Homo sapiens 114-118 8072693-9 1994 In Fura-2-loaded isolated sympathetic neurites obtained from superior cervical ganglia explants, the depolarization-induced (54 mM KCl) increase in cytosolic calcium was attenuated by neuropeptide Y (50 nM). Fura-2 3-9 neuropeptide Y Rattus norvegicus 184-198 8091392-2 1994 Basal and thrombin-induced increases in platelet intracellular free calcium ion concentration ([Ca2+]i; fura-2) were similar in hyperlipidemic patients and controls (45 +/- 5 vs 42 +/- 3 and 635 +/- 51 vs 599 +/- 69 mM, respectively). Fura-2 104-110 coagulation factor II, thrombin Homo sapiens 10-18 8190099-5 1994 The fMLP- or C5a-induced calcium mobilization form intracellular stores of fura-2-loaded cells, as well as the fMLP- or A23187-triggered release of [14C] arachidonate from prelabeled neutrophils, was not affected by B-003. Fura-2 75-81 formyl peptide receptor 1 Homo sapiens 4-8 7512352-1 1994 Depolarization-induced intracellular Ca2+ rises were measured in fura-2-loaded, voltage-clamped Purkinje cells. Fura-2 65-71 carbonic anhydrase 2 Homo sapiens 37-40 7545917-3 1994 Modulation of cellular phenotype by EMAP II-derived peptide was suggested by peptide-induced elevation of cytosolic free calcium concentration in fura-2-loaded MPs and PMNs and by stimulation of peroxidase release in PMNs. Fura-2 146-152 aminoacyl tRNA synthetase complex interacting multifunctional protein 1 Homo sapiens 36-43 8132547-6 1994 Mn2+ induced a quench of the fura-2 fluorescence, which ceased promptly upon the removal of NPY, indicating that Ca2+ entry was linked tightly to receptor activation. Fura-2 29-35 neuropeptide Y Homo sapiens 92-95 7510965-5 1994 The effect of these two drugs upon (Ca2+)i changes was measured using the fluorescent probe fura-2 loaded into RBL-2H3 cells passively sensitized with DNP-specific IgE. Fura-2 92-98 carbonic anhydrase 2 Rattus norvegicus 36-39 7510965-5 1994 The effect of these two drugs upon (Ca2+)i changes was measured using the fluorescent probe fura-2 loaded into RBL-2H3 cells passively sensitized with DNP-specific IgE. Fura-2 92-98 immunoglobulin heavy constant epsilon Homo sapiens 164-167 8117446-2 1994 In fura-2-loaded cells, PAF (10(-9) to 10(-5) M), but not lyso-PAF, increased [Ca2+]i in a concentration-dependent manner, from 106 +/- 15 to 270 +/- 40 nM (P < 0.05). Fura-2 3-9 PCNA-associated factor Bos taurus 24-27 8187959-1 1994 Luteinizing hormone (LH)/chorionic gonadotropin (CG) receptor (LHR) regulation of intracellular free Ca2+, [Ca2+]i, was studied by spectrofluorometric analysis and digital imaging of intracellularly trapped fura-2 fluorescence in a stable cell line 293 expressing transfected rat LHR. Fura-2 207-213 chorionic gonadotropin subunit beta 5 Homo sapiens 49-51 8149415-3 1994 Pretreatment of Fura-2 loaded cells with 4-bromophenylacyl, an inhibitor of phospholipase A2, had no effect on the ATP-induced entry of Ca2+ but inhibited the release of sequestered Ca2+. Fura-2 16-22 phospholipase A2 group IB Rattus norvegicus 76-92 8293556-7 1994 By use of Mn2+ quenching of intracellular fura 2 to measure divalent cation entry, the maximal rate of vasopressin-stimulated Mn2+ entry was impaired in simvastatin-treated cells by 52%. Fura-2 42-48 arginine vasopressin Rattus norvegicus 103-114 7513035-4 1994 In separate experiments, [Ca2+]i changes in response to IGF-I were measured spectrofluorometrically in fura-2-loaded cells. Fura-2 103-109 insulin like growth factor 1 Homo sapiens 56-61 8187959-1 1994 Luteinizing hormone (LH)/chorionic gonadotropin (CG) receptor (LHR) regulation of intracellular free Ca2+, [Ca2+]i, was studied by spectrofluorometric analysis and digital imaging of intracellularly trapped fura-2 fluorescence in a stable cell line 293 expressing transfected rat LHR. Fura-2 207-213 luteinizing hormone/choriogonadotropin receptor Rattus norvegicus 63-66 8275927-2 1994 Video image analysis of fura-2-loaded cells revealed that PTH (10(-7) M) induced transient increases of [Ca2+]i preferentially in cells in S phase (82% response frequency, n = 63; 286 +/- 33% of baseline, n = 29), whereas cells in G1 phase responded poorly to PTH (10% response frequency, n = 51; 140 +/- 8% of baseline, n = 5). Fura-2 24-30 parathyroid hormone Rattus norvegicus 58-61 8293807-1 1994 Regulation of the cytoplasmic calcium concentration ([Ca2+]i) was studied in fura-2-loaded C-cells from two human medullary thyroid carcinomas (MTC). Fura-2 77-83 carbonic anhydrase 2 Homo sapiens 54-57 8124662-1 1994 Angiotensin II (Ang II), bradykinin (BK), and endothelin-1 (ET-1) evoked alterations in cytosolic free calcium, [Ca2+]i, levels were determined using fura-2 fluorescence methodology in a human lung adenocarcinoma cell line (A549), a non-neoplastic lung cell line and a small cell lung carcinoma cell (SCLC) line. Fura-2 150-156 angiotensinogen Homo sapiens 0-14 8124662-1 1994 Angiotensin II (Ang II), bradykinin (BK), and endothelin-1 (ET-1) evoked alterations in cytosolic free calcium, [Ca2+]i, levels were determined using fura-2 fluorescence methodology in a human lung adenocarcinoma cell line (A549), a non-neoplastic lung cell line and a small cell lung carcinoma cell (SCLC) line. Fura-2 150-156 endothelin 1 Homo sapiens 46-58 8124662-1 1994 Angiotensin II (Ang II), bradykinin (BK), and endothelin-1 (ET-1) evoked alterations in cytosolic free calcium, [Ca2+]i, levels were determined using fura-2 fluorescence methodology in a human lung adenocarcinoma cell line (A549), a non-neoplastic lung cell line and a small cell lung carcinoma cell (SCLC) line. Fura-2 150-156 endothelin 1 Homo sapiens 60-64 8275927-2 1994 Video image analysis of fura-2-loaded cells revealed that PTH (10(-7) M) induced transient increases of [Ca2+]i preferentially in cells in S phase (82% response frequency, n = 63; 286 +/- 33% of baseline, n = 29), whereas cells in G1 phase responded poorly to PTH (10% response frequency, n = 51; 140 +/- 8% of baseline, n = 5). Fura-2 24-30 parathyroid hormone Rattus norvegicus 260-263 8300340-4 1994 Endothelin receptor linkage to cell signaling pathways was determined based on measurements of the dose dependent effects of ET-1, ET-2, and ET-3 on intracellular Ca2+ concentration ([Ca2+]i) transients in fura-2-loaded cells, and of ET-1 on phosphoinositide turnover and cAMP accumulation in the isolated rabbit corneal epithelium. Fura-2 206-212 endothelin-1 Oryctolagus cuniculus 125-129 8001869-5 1994 Interleukin-1 beta (IL-1 beta) and tumor necrosis factor-beta (TNF-beta) led to a significant increase in relative fluorescence release (RFR) of fura-2 (p < 0.05 and p < 0.02, respectively) while endotoxin did not show significant changes of RFR of fura-2. Fura-2 145-151 interleukin 1 beta Homo sapiens 0-18 8001869-5 1994 Interleukin-1 beta (IL-1 beta) and tumor necrosis factor-beta (TNF-beta) led to a significant increase in relative fluorescence release (RFR) of fura-2 (p < 0.05 and p < 0.02, respectively) while endotoxin did not show significant changes of RFR of fura-2. Fura-2 145-151 interleukin 1 beta Homo sapiens 20-29 8001869-5 1994 Interleukin-1 beta (IL-1 beta) and tumor necrosis factor-beta (TNF-beta) led to a significant increase in relative fluorescence release (RFR) of fura-2 (p < 0.05 and p < 0.02, respectively) while endotoxin did not show significant changes of RFR of fura-2. Fura-2 145-151 lymphotoxin alpha Homo sapiens 35-61 8001869-5 1994 Interleukin-1 beta (IL-1 beta) and tumor necrosis factor-beta (TNF-beta) led to a significant increase in relative fluorescence release (RFR) of fura-2 (p < 0.05 and p < 0.02, respectively) while endotoxin did not show significant changes of RFR of fura-2. Fura-2 145-151 lymphotoxin alpha Homo sapiens 63-71 8001869-5 1994 Interleukin-1 beta (IL-1 beta) and tumor necrosis factor-beta (TNF-beta) led to a significant increase in relative fluorescence release (RFR) of fura-2 (p < 0.05 and p < 0.02, respectively) while endotoxin did not show significant changes of RFR of fura-2. Fura-2 255-261 interleukin 1 beta Homo sapiens 0-18 8001869-5 1994 Interleukin-1 beta (IL-1 beta) and tumor necrosis factor-beta (TNF-beta) led to a significant increase in relative fluorescence release (RFR) of fura-2 (p < 0.05 and p < 0.02, respectively) while endotoxin did not show significant changes of RFR of fura-2. Fura-2 255-261 interleukin 1 beta Homo sapiens 20-29 8001869-5 1994 Interleukin-1 beta (IL-1 beta) and tumor necrosis factor-beta (TNF-beta) led to a significant increase in relative fluorescence release (RFR) of fura-2 (p < 0.05 and p < 0.02, respectively) while endotoxin did not show significant changes of RFR of fura-2. Fura-2 255-261 lymphotoxin alpha Homo sapiens 35-61 8001869-5 1994 Interleukin-1 beta (IL-1 beta) and tumor necrosis factor-beta (TNF-beta) led to a significant increase in relative fluorescence release (RFR) of fura-2 (p < 0.05 and p < 0.02, respectively) while endotoxin did not show significant changes of RFR of fura-2. Fura-2 255-261 lymphotoxin alpha Homo sapiens 63-71 8300340-4 1994 Endothelin receptor linkage to cell signaling pathways was determined based on measurements of the dose dependent effects of ET-1, ET-2, and ET-3 on intracellular Ca2+ concentration ([Ca2+]i) transients in fura-2-loaded cells, and of ET-1 on phosphoinositide turnover and cAMP accumulation in the isolated rabbit corneal epithelium. Fura-2 206-212 endothelin-2 Oryctolagus cuniculus 131-135 8300340-4 1994 Endothelin receptor linkage to cell signaling pathways was determined based on measurements of the dose dependent effects of ET-1, ET-2, and ET-3 on intracellular Ca2+ concentration ([Ca2+]i) transients in fura-2-loaded cells, and of ET-1 on phosphoinositide turnover and cAMP accumulation in the isolated rabbit corneal epithelium. Fura-2 206-212 endothelin-3 Oryctolagus cuniculus 141-145 8300340-4 1994 Endothelin receptor linkage to cell signaling pathways was determined based on measurements of the dose dependent effects of ET-1, ET-2, and ET-3 on intracellular Ca2+ concentration ([Ca2+]i) transients in fura-2-loaded cells, and of ET-1 on phosphoinositide turnover and cAMP accumulation in the isolated rabbit corneal epithelium. Fura-2 206-212 endothelin-1 Oryctolagus cuniculus 234-238 7506746-3 1994 The elevation in cytosolic calcium, as detected in Fura-2-labeled basophils, after challenge with anti-IgE or fMLP showed graded characteristics in that the distributions were unimodal under conditions of optimal or suboptimal challenge with little skewing from a normal distribution. Fura-2 51-57 formyl peptide receptor 1 Homo sapiens 110-114 8115999-1 1993 The effect of type IIB von Willebrand"s factor (vWF) on platelet cytosolic Ca2+ ion concentration, measured by means of the probe fura 2, was investigated. Fura-2 130-136 von Willebrand factor Homo sapiens 48-51 7507268-8 1994 Fura 2 was used to measure intracellular free Cd2+ by fluorescence ratio methods. Fura-2 0-6 Cd2 molecule Rattus norvegicus 46-49 7507268-10 1994 Cd2+ addition resulted in a gradual increase in Fura 2 fluorescence in both PC12 and PC18 cells, but depolarization with BAY K8644 increased the apparent rate of Cd2+ uptake only for the PC12 cells. Fura-2 48-54 Cd2 molecule Rattus norvegicus 0-3 8087627-3 1993 Therefore, we studied the effect of BK, and its putative modulation by tumor necrosis factor alpha (TNF alpha), on cytosolic free Ca2+ concentration ([Ca2+]i) in fura-2-loaded tracheal smooth muscle cells in culture. Fura-2 162-168 tumor necrosis factor Cavia porcellus 100-109 8145147-12 1993 In fura-2-loaded unclamped cells, TRH induced either release of Ca2+ from internal stores, Ca2+ entry, or both. Fura-2 3-9 thyrotropin releasing hormone Cavia porcellus 34-37 8119327-2 1993 Endothelin-3 in a range of 0.1-100 pM induced arteriolar constriction in a dose-dependent manner, and stimulated Ca2+ mobilization, demonstrated by fura-2-associated fluorography, in both arterioles and venules. Fura-2 148-154 endothelin 3 Rattus norvegicus 0-12 8120825-11 1993 When the internal concentration of calcium ions, [Ca2+]i, was measured using fura-2, brief transmural stimuli caused an increase in [Ca2+]i. Fura-2 77-83 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 50-53 8120825-11 1993 When the internal concentration of calcium ions, [Ca2+]i, was measured using fura-2, brief transmural stimuli caused an increase in [Ca2+]i. Fura-2 77-83 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 133-136 7997069-4 1994 The results in vitro using the Ca(2+)-sensitive dye fura-2 showed that nimodipine reduced in a dose-dependent manner the elevation of [Ca2+]i in hippocampal neurons induced by K(+)-stimulation. Fura-2 52-58 carbonic anhydrase 2 Rattus norvegicus 135-138 8262215-4 1993 Additionally, extracellular application of Ba2+ increased the apparent internal Ca2+ concentration ([Ca2+]app) in fura-2-loaded chromaffin cells. Fura-2 114-120 carbonic anhydrase 2 Bos taurus 80-83 8262215-4 1993 Additionally, extracellular application of Ba2+ increased the apparent internal Ca2+ concentration ([Ca2+]app) in fura-2-loaded chromaffin cells. Fura-2 114-120 carbonic anhydrase 2 Bos taurus 101-104 8262215-11 1993 In some cases, Ba2+ additionally induces release of Ca2+ from internal stores, as evidenced by its effect on fura-2 fluorescence at different wavelengths. Fura-2 109-115 carbonic anhydrase 2 Bos taurus 52-55 8227034-4 1993 Studies in fura-2/AM-loaded cells showed that concentrations of thapsigargin that elicited PAF production induced a protracted and long lasting elevation of cytosolic free calcium concentration ([Ca2+]i) between 200 and 700 nM. Fura-2 11-17 PCNA clamp associated factor Homo sapiens 91-94 8288312-8 1993 Fura-2 analysis demonstrated that PMA-induced PKC translocation was not accompanied by a net change in cytosolic calcium levels. Fura-2 0-6 proline rich transmembrane protein 2 Homo sapiens 46-49 8077313-2 1993 In the present study we used fura-2 microfluorimetry to investigate whether activation of the oxytocin receptor of cultured human granulosa-lutein cells causes intracellular calcium (Ca2+) signals and affects progesterone release. Fura-2 29-35 oxytocin/neurophysin I prepropeptide Homo sapiens 94-102 8226749-3 1993 [Ca2+]c was monitored by video imaging of single fura-2-loaded INS-1 cells, or in populations of cells transfected with non-targeted (cytosolic) aequorin. Fura-2 49-55 insulin 1 Rattus norvegicus 63-68 8407906-2 1993 Single cell intracellular Ca2+ responses to Pg and u-PA were studied using Fura-2 and digital imaging fluorescence microscopy. Fura-2 75-81 plasminogen activator, urokinase Homo sapiens 51-55 7688740-0 1993 ADP- and thapsigargin-evoked Ca2+ entry and protein-tyrosine phosphorylation are inhibited by the tyrosine kinase inhibitors genistein and methyl-2,5-dihydroxycinnamate in fura-2-loaded human platelets. Fura-2 172-178 WD and tetratricopeptide repeats 1 Homo sapiens 0-4 8247351-5 1993 Intracellular Ca2+ levels were monitored using the Ca2+ indicator Fura-2 and were found to rise in both hair cell types in response to ATP. Fura-2 66-72 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 14-17 8247351-5 1993 Intracellular Ca2+ levels were monitored using the Ca2+ indicator Fura-2 and were found to rise in both hair cell types in response to ATP. Fura-2 66-72 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 51-54 8376384-2 1993 We report that CCK-JMV-180 can accelerate Ca2+ influx into fura-2-loaded dispersed rat pancreatic acini and single acinar cells. Fura-2 59-65 cholecystokinin Rattus norvegicus 15-18 8357814-0 1993 Fura-2 transport in toad urinary bladder epithelium: effects of antidiuretic hormone, colchicine and osmotic gradients. Fura-2 0-6 arginine vasopressin Homo sapiens 64-84 8240466-9 1993 Studies with the fluorescent calcium chelating dye FURA 2/AM showed that nimesulide was able to reduce free cytosolic calcium increase produced by fMLP and the ionophore ionomycin. Fura-2 51-57 formyl peptide receptor 1 Homo sapiens 147-151 8220881-2 1993 In Fura-2 preloaded human platelets, the increase in cytosolic calcium induced by alpha-thrombin was reduced by some L- and D-arginine ester compounds the IC50 (microM) values of which were 7.4 for TAEE, 56.9 for BAEE, 77.6 for TAME, 560 for T(d)AME, 656.3 for L-ArgOMe and 2206.7 for D-ArgOMe. Fura-2 3-9 coagulation factor II, thrombin Homo sapiens 88-96 8255728-5 1993 The net electrochemical gradient for ionic movements through the Na+/Ca2+ exchanger, during the course of the response of macrophages to PAF, was determined by combining the measurements of membrane potential (in patch-clamp), of [Ca2+]i (with fura-2), and of the intracellular Na+ concentration (with sodium-binding benzofuran isophthalate). Fura-2 244-250 solute carrier family 8 member A1 Homo sapiens 65-83 8394831-2 1993 Activin A increased intracellular the free Ca2+ concentration, [Ca2+]i in fura-2-loaded islets. Fura-2 74-80 inhibin subunit beta A Rattus norvegicus 0-9 8368272-3 1993 Changes in pHi and Cai were measured with the fluorescent probes 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein and fura 2. Fura-2 116-122 glucose-6-phosphate isomerase Rattus norvegicus 11-14 7686480-7 1993 Digital fluorescence imaging of individual fura-2-loaded cells confirmed that the average [Ca2+]i response to depolarization was lower in cells that had been incubated with EGF for 36 h. EGF treatment increased the amount of PRL secreted basally, but inhibited the acute PRL secretory response to depolarization with 50 mM KCl and 1 microM BAY K8644 from 2.6- to 1.5-fold. Fura-2 43-49 epidermal growth factor like 1 Rattus norvegicus 173-176 8394074-5 1993 Additional studies in which the extracellular and intracellular concentrations of Ca2+ were varied indicated that maximal LTB4-induced PLD activation was dependent upon Ca2+ and potentiated by inhibitors of protein kinase C. The time-course and concentration-response curves for LTB4-induced PLD activation were different from those for LTB4-induced Ca2+ mobilization, as measured by fura-2 fluorescence. Fura-2 384-390 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 135-138 8252582-4 1993 METHODS: The effect of erythropoietin on ([Ca2+]i was measured with the fluorescent dye fura2 in cultured vascular smooth muscle cells from Wistar Kyoto rats. Fura-2 88-93 erythropoietin Rattus norvegicus 23-37 8319379-3 1993 We found that purinoceptor agonists, including ATP and its analogs, and bombesin, an amphibian tetradeca-peptide of mammalian homology gastrin-releasing peptide, induced rapid transient increase of cytoplasmic-free Ca2+ in PG cells loaded with fura-2. Fura-2 244-250 gastrin releasing peptide Homo sapiens 72-80 7686480-7 1993 Digital fluorescence imaging of individual fura-2-loaded cells confirmed that the average [Ca2+]i response to depolarization was lower in cells that had been incubated with EGF for 36 h. EGF treatment increased the amount of PRL secreted basally, but inhibited the acute PRL secretory response to depolarization with 50 mM KCl and 1 microM BAY K8644 from 2.6- to 1.5-fold. Fura-2 43-49 epidermal growth factor like 1 Rattus norvegicus 187-190 7684899-5 1993 In addition, SP-(7-11), but not intact SP, SP-(1-4), SP-(1-6), SP-(8-11) or SP-(9-11), nor the tachykinins NKA and NKB, caused an increase in the intracellular calcium concentration as measured by the fluorescent dye Fura-2. Fura-2 217-223 tachykinin precursor 1 Bos taurus 13-15 8390453-1 1993 Emptying the intracellular calcium stores of fura-2-loaded human neutrophils by treatment with the endomembrane ATPase inhibitor thapsigargin leads to a maintained increase of [Ca2+]i by Ca2+ entry through a store-operated Ca2+ entry pathway. Fura-2 45-51 dynein axonemal heavy chain 8 Homo sapiens 112-118 8395337-1 1993 Free calcium concentration in isolated single neurons was clamped using a new technical approach based on a feed-back connection between the Fura-2 fluorescence signal measuring the intracellular Ca2+ concentration ([Ca2+]i) and iontophoretic current injecting Ca2+ into the cell. Fura-2 141-147 carbonic anhydrase 2 Homo sapiens 196-199 8395337-1 1993 Free calcium concentration in isolated single neurons was clamped using a new technical approach based on a feed-back connection between the Fura-2 fluorescence signal measuring the intracellular Ca2+ concentration ([Ca2+]i) and iontophoretic current injecting Ca2+ into the cell. Fura-2 141-147 carbonic anhydrase 2 Homo sapiens 217-220 8395337-1 1993 Free calcium concentration in isolated single neurons was clamped using a new technical approach based on a feed-back connection between the Fura-2 fluorescence signal measuring the intracellular Ca2+ concentration ([Ca2+]i) and iontophoretic current injecting Ca2+ into the cell. Fura-2 141-147 carbonic anhydrase 2 Homo sapiens 217-220 8411123-3 1993 We investigated PAF-induced Ca2+ signaling in HMDMs using the calcium indicator Fura-2, combining single cell ratio fluorimetry and digital video imaging with whole-cell recording techniques. Fura-2 80-86 PCNA clamp associated factor Homo sapiens 16-19 8335062-2 1993 In this paper, changes in intracellular Ca2+ concentration ([Ca2+]i) in response to CCK (cholecystokinin) peptides were measured in the Human JURKAT T Lymphocyte Cell line by fura-2 fluorometry. Fura-2 175-181 cholecystokinin Homo sapiens 84-87 8335062-2 1993 In this paper, changes in intracellular Ca2+ concentration ([Ca2+]i) in response to CCK (cholecystokinin) peptides were measured in the Human JURKAT T Lymphocyte Cell line by fura-2 fluorometry. Fura-2 175-181 cholecystokinin Homo sapiens 89-104 8397354-1 1993 Intracellular calcium levels were measured by ratio imaging of Fura-2-injected CA1 pyramidal neurons during repetitive electrical stimulation of the Schaffer collaterals in rat hippocampal slices. Fura-2 63-69 carbonic anhydrase 1 Rattus norvegicus 79-82 8261130-7 1993 Combined voltage-clamp recordings and microfluorometric measurements of [Ca2+]i using the Ca2+ indicator fura-2 revealed that the amplitude of IADP was correlated with the amplitude of depolarization-induced Ca2+ influx. Fura-2 105-111 carbonic anhydrase 2 Rattus norvegicus 73-76 8261130-7 1993 Combined voltage-clamp recordings and microfluorometric measurements of [Ca2+]i using the Ca2+ indicator fura-2 revealed that the amplitude of IADP was correlated with the amplitude of depolarization-induced Ca2+ influx. Fura-2 105-111 carbonic anhydrase 2 Rattus norvegicus 90-93 8261130-7 1993 Combined voltage-clamp recordings and microfluorometric measurements of [Ca2+]i using the Ca2+ indicator fura-2 revealed that the amplitude of IADP was correlated with the amplitude of depolarization-induced Ca2+ influx. Fura-2 105-111 carbonic anhydrase 2 Rattus norvegicus 90-93 8386174-3 1993 Fluorescence measurements using Fura-2 show that at pH 7.4, the Ca2+ requirement for gelsolin activation in vitro is higher than previously reported, with half-maximal activation of severing and nucleation occurring at 10 microM Ca2+. Fura-2 32-38 gelsolin Homo sapiens 85-93 8392939-2 1993 Fura-2-loaded and gel-filtered platelets were stimulated by either ADP (8 microM), thrombin (0.025 IU/ml) or collagen (1-3 micrograms/ml), respectively. Fura-2 0-6 coagulation factor II, thrombin Homo sapiens 83-91 8393065-5 1993 The intracellular [Ca2+] (measured with fura-2) was significantly increased, from 125 nM (control) to 335 nM (0 mM K+ bath) or 408 nM (0.2 mM strophanthidin), during inhibition of pump activity. Fura-2 40-46 carbonic anhydrase 2 Rattus norvegicus 19-22 8329674-1 1993 The isolated rat renal arteriole technique was adapted for use in a fluorescence ratio imaging system in which angiotensin II (AII)-induced changes in lumen diameter and smooth muscle cell (SMC) cytosolic calcium [(Ca2+]i were serially determined for 4 min with Fura-2. Fura-2 262-268 angiotensinogen Rattus norvegicus 111-125 8329674-1 1993 The isolated rat renal arteriole technique was adapted for use in a fluorescence ratio imaging system in which angiotensin II (AII)-induced changes in lumen diameter and smooth muscle cell (SMC) cytosolic calcium [(Ca2+]i were serially determined for 4 min with Fura-2. Fura-2 262-268 angiotensinogen Rattus norvegicus 127-130 8387925-5 1993 Fluorescent ratio imaging with fura-2 revealed that addition of vasopressin to the cells results in an increase of [Ca2+]i which peaks within 20 s and does not return to resting levels during the 100 s observation period. Fura-2 31-37 arginine vasopressin Rattus norvegicus 64-75 8491259-1 1993 The rare earth lanthanide gadolinium (Gd3+), in concentrations ranging from 1 to 100 microM, reduced the elevation of intracellular Ca2+ concentration [Ca2+]i, monitored by means of the fluorescent probe fura-2. Fura-2 204-210 GRDX Homo sapiens 38-41 8384995-4 1993 When the intracellular free Ca2+ concentration ([Ca2+]i) is measured in a fura 2-loaded single chromaffin cell, PACAP is found to cause a sustained increase in [Ca2+]i by mobilizing Ca2+ from both extra- and intracellular pools. Fura-2 74-80 adenylate cyclase activating polypeptide 1 Homo sapiens 112-117 7504109-4 1993 The amount of intracellular free Ca2+ ([Ca2+]i) was fluorometrically measured using fura-2. Fura-2 84-90 carbonic anhydrase 2 Rattus norvegicus 33-36 7504109-4 1993 The amount of intracellular free Ca2+ ([Ca2+]i) was fluorometrically measured using fura-2. Fura-2 84-90 carbonic anhydrase 2 Rattus norvegicus 40-43 7504109-20 1993 Fura-2 imaging showed that [Ca2+]i was increased when ACh was added to the external medium with or without Ca2+, though in Ca(2+)-free medium only the first application of ACh increased the [Ca2+]i. Fura-2 0-6 carbonic anhydrase 2 Rattus norvegicus 28-31 7504109-20 1993 Fura-2 imaging showed that [Ca2+]i was increased when ACh was added to the external medium with or without Ca2+, though in Ca(2+)-free medium only the first application of ACh increased the [Ca2+]i. Fura-2 0-6 carbonic anhydrase 2 Rattus norvegicus 107-110 7504109-20 1993 Fura-2 imaging showed that [Ca2+]i was increased when ACh was added to the external medium with or without Ca2+, though in Ca(2+)-free medium only the first application of ACh increased the [Ca2+]i. Fura-2 0-6 carbonic anhydrase 2 Rattus norvegicus 107-110 8382636-2 1993 The flux through ROCCs was followed by quenching of fura-2 fluorescence due to the influx of extracellular Mn2+ induced by vasopressin. Fura-2 52-58 arginine vasopressin Rattus norvegicus 123-134 8384417-10 1993 Cai was measured using the fluorescence of the Ca indicator fura-2 in principal cells of split tubules under conditions identical to those used for electrical measurements. Fura-2 60-66 carbonic anhydrase 1 Rattus norvegicus 0-3 7679080-0 1993 Calcium influx through annexin V ion channels into large unilamellar vesicles measured with fura-2. Fura-2 92-98 annexin A5 Homo sapiens 23-32 8383969-2 1993 [Ca2+]i and pHi were measured using the fluorescent dyes fura-2 and BCECF [2",7"-bis-(carboxyethyl)-5,6-carboxyfluorescein] respectively. Fura-2 57-63 glucose-6-phosphate isomerase Homo sapiens 12-15 8445010-5 1993 Preincubation of hirudinized platelet-rich plasma with insulin had a dose- and time-dependent effect on the increase of [Mg2+]i measured in Mag-fura-2-loaded cells with a fluorescence spectrophotometer. Fura-2 144-150 insulin Homo sapiens 55-62 8382265-3 1993 FTX (1:4,000 dilution, with respect to the original fraction) inhibited K(+)-depolarization-induced Ca2+ influx by 50%, as monitored with fura-2, whereas nitrendipine (0.1-1 microM) and FTX (3:3), a synthetic FTX analogue (1 mM), blocked the [Ca2+]i transients by 35 and 30%, respectively. Fura-2 138-144 FTX transcript, XIST regulator Homo sapiens 0-3 8510321-1 1993 This study was aimed at determining whether the thrombin (TRB)-induced aggregation and intracellular free Ca2+ ([Ca(2+)]i) mobilization of fura-2-loaded human platelets would be affected by aminoglycoside antibiotics (AGs), i.e., kanamycin (KM), gentamicin (GM) and fradiomycin (FRM) with 4, 5 and 6 amino groups in their molecules, respectively. Fura-2 139-145 coagulation factor II, thrombin Homo sapiens 48-56 8510321-1 1993 This study was aimed at determining whether the thrombin (TRB)-induced aggregation and intracellular free Ca2+ ([Ca(2+)]i) mobilization of fura-2-loaded human platelets would be affected by aminoglycoside antibiotics (AGs), i.e., kanamycin (KM), gentamicin (GM) and fradiomycin (FRM) with 4, 5 and 6 amino groups in their molecules, respectively. Fura-2 139-145 coagulation factor II, thrombin Homo sapiens 58-61 7679430-3 1993 Agonist-stimulated Ca2+ influx was demonstrated directly by the use of Mn2+: in the presence of extracellular Mn2+, MCP-1 and FMLP stimulated a dose-dependent quench in fluorescence of Fura-2-loaded monocytes. Fura-2 185-191 C-C motif chemokine ligand 2 Homo sapiens 116-121 7679430-3 1993 Agonist-stimulated Ca2+ influx was demonstrated directly by the use of Mn2+: in the presence of extracellular Mn2+, MCP-1 and FMLP stimulated a dose-dependent quench in fluorescence of Fura-2-loaded monocytes. Fura-2 185-191 formyl peptide receptor 1 Homo sapiens 126-130 7679080-4 1993 The resulting calcium influx into the LUV detected through the fluorescence changes of fura-2 was used as a qualitative test for the electrophysiological properties of annexin V. Fura-2 87-93 annexin A5 Homo sapiens 168-177 8419539-5 1993 Experiments of [Ca2+]i measurement with the fura-2 technique showed that P2y agonists, as well as bradykinin, were able to increase [Ca2+]i, this effect being independent of the presence of extracellular Ca2+. Fura-2 44-50 kininogen 1 Homo sapiens 98-108 8435086-17 1993 Finally, use of fura-2 and SK&F 96365 to manipulate the fMLP-stimulated rise in [Ca2+]i showed that when fMLP was able to evoke its normal rise in [Ca2+]i (to a peak of 700-900 nM), O2-. Fura-2 16-22 formyl peptide receptor 1 Homo sapiens 60-64 8435086-17 1993 Finally, use of fura-2 and SK&F 96365 to manipulate the fMLP-stimulated rise in [Ca2+]i showed that when fMLP was able to evoke its normal rise in [Ca2+]i (to a peak of 700-900 nM), O2-. Fura-2 16-22 formyl peptide receptor 1 Homo sapiens 109-113 7679387-1 1993 Intracellular calcium ([Ca2+]i) was measured in FURA 2-loaded endothelial cells plated on fibronectin or vitronectin. Fura-2 48-54 fibronectin 1 Homo sapiens 90-101 7679387-1 1993 Intracellular calcium ([Ca2+]i) was measured in FURA 2-loaded endothelial cells plated on fibronectin or vitronectin. Fura-2 48-54 vitronectin Homo sapiens 105-116 8450563-7 1993 As detected by single cell microfluorimetric (fura-2) measurements neuropeptide Y induced an increase of [Ca2+]i which was caused by the entry of extracellular Ca2+. Fura-2 46-52 neuropeptide Y Rattus norvegicus 67-81 8275798-1 1993 Platelet-activating factor (PAF) elicited an increase in intracellular Ca2+ concentration, [Ca2+]i, in neuroblastoma x glioma hybrid NG 108-15 cells as measured by fura-2 fluorescence method. Fura-2 164-170 patchy fur Mus musculus 0-26 8422259-1 1993 Incubating Fura 2 loaded thyroid FRTL-5 cells with the calmodulin inhibitor W-7 decreased the ATP-evoked increase in intracellular free calcium. Fura-2 11-17 calmodulin 1 Rattus norvegicus 55-65 8422913-3 1993 Here we have investigated the effects of two tyrosine kinase inhibitors, methyl 2,5-dihydroxycinnamate and genistein, on thrombin-evoked protein tyrosine phosphorylation and Ca2+ signal generation in fura-2-loaded human platelets. Fura-2 200-206 coagulation factor II, thrombin Homo sapiens 121-129 8420790-3 1993 Gel-filtered and fura-2-loaded platelets were stimulated with ADP (4-8 microM), collagen (2-10 micrograms/ml) or thrombin (0.02-0.05 IU/ml), respectively. Fura-2 17-23 coagulation factor II, thrombin Homo sapiens 113-121 8275798-1 1993 Platelet-activating factor (PAF) elicited an increase in intracellular Ca2+ concentration, [Ca2+]i, in neuroblastoma x glioma hybrid NG 108-15 cells as measured by fura-2 fluorescence method. Fura-2 164-170 patchy fur Mus musculus 28-31 1471987-2 1992 The [Ca2+]i rises elicited by low (50-80 nM) cathepsin G concentrations in fura-2-loaded platelets showed a biphasic mode, with a first small peak followed by a greater and more prolonged Ca2+ transient. Fura-2 75-81 cathepsin G Homo sapiens 45-56 1361128-1 1992 Changes in intracellular Ca2+ concentration ([Ca2+]i) in the soma and dendrites of hippocampal CA1 pyramidal neurons were measured using intracellularly injected fura-2. Fura-2 162-168 carbonic anhydrase 1 Homo sapiens 95-98 1336985-4 1992 It also reduced the size of the PAF-induced calcium (Ca2+) transient demonstrated in Fura-2 loaded platelets. Fura-2 85-91 PCNA clamp associated factor Homo sapiens 32-35 1327522-3 1992 We found that the addition of gastrin to small cell lung cancer cells loaded with the fluorescent Ca2+ indicator fura 2-tetraacetoxymethylester causes a rapid and transient increase in the intracellular concentration of Ca2+ ([Ca2+]i) followed by homologous desensitization. Fura-2 113-119 gastrin Homo sapiens 30-37 1491443-5 1992 Fura-2/AM-loaded nephron segments reveal a transient increase of cytosolic free calcium concentrations by agonists such as angiotensin II, vasopressin, kinins, etc. Fura-2 0-6 angiotensinogen Homo sapiens 123-137 1491443-5 1992 Fura-2/AM-loaded nephron segments reveal a transient increase of cytosolic free calcium concentrations by agonists such as angiotensin II, vasopressin, kinins, etc. Fura-2 0-6 arginine vasopressin Homo sapiens 139-150 1332716-6 1992 Thus lipoproteins, like Ang II and PDGF-BB, activate polyphosphatidylinositol-specific phospholipase C. Intracellular Ca2+ concentrations ([Ca2+]i) were studied in fura-2 loaded VSMC. Fura-2 164-170 angiotensinogen Homo sapiens 24-30 1400356-2 1992 To elucidate the effects of endothelium-derived relaxing factor (EDRF) released from vascular endothelial cells (ECs) on handling of intracellular calcium ion (Ca2+i) in ECs themselves and vascular smooth muscle cells (VSMCs), we measured the Ca2+i by two-dimensional digital image analysis of fura-2-loaded ECs and VSMCs in tissue culture. Fura-2 294-300 alpha hemoglobin stabilizing protein Homo sapiens 65-69 1331121-1 1992 We have previously reported that platelet-activating factor (PAF) elevates cytosolic free calcium concentration ([Ca2+]i) in fura-2-loaded glomerular mesangial cells. Fura-2 125-131 PCNA clamp associated factor Rattus norvegicus 33-59 1331121-1 1992 We have previously reported that platelet-activating factor (PAF) elevates cytosolic free calcium concentration ([Ca2+]i) in fura-2-loaded glomerular mesangial cells. Fura-2 125-131 PCNA clamp associated factor Rattus norvegicus 61-64 1335472-9 1992 When the relationship between pHi and intracellular Ca2+ was determined with the fluorescent Ca2+ indicator, Fura-2, it was found that increasing pHo and treatment with ammonium chloride increased intracellular Ca2+, while sodium propionate and reducing pHi had no effect on intracellular Ca2+. Fura-2 109-115 glucose-6-phosphate isomerase Rattus norvegicus 30-33 1359030-2 1992 We have used microfluorimetry of the calcium indicator fura-2 (Grynkiewicz et al., 1985) to measure and characterize synaptically activated calcium changes in individual CA1 pyramidal cells contained within guinea pig hippocampal slices. Fura-2 55-61 carbonic anhydrase 1 Cavia porcellus 170-173 1440710-3 1992 METHODS: The fluorescent indicators fura-2 and bis-carboxyethyl-carboxyfluorescein were used to simultaneously measure [Ca2+]i and pHi with a fluorometer. Fura-2 36-42 glucose-6-phosphate isomerase Rattus norvegicus 131-134 1362150-1 1992 Changes in the membrane potential and the intracellular Ca2+ concentration ([Ca2+]i) caused by somatostatin (SRIF) were simultaneously measured in human GH-producing pituitary tumor cells, by means of the nystatin-perforated whole cell clamp technique and Fura-2 AM. Fura-2 256-262 somatostatin Homo sapiens 95-107 1388192-2 1992 Co-cross-linking of CD4 and Fc gamma R by an anti-CD4 mAb (MAX.16H5) and by excess of goat anti-mouse Ig induced a biphasic increase of the free cytosolic Ca(2+)-concentration ([Ca2+]i) in the human monocytoid cell line U937 as measured by FURA-2 fluorescence. Fura-2 240-246 CD4 molecule Homo sapiens 20-23 1388192-2 1992 Co-cross-linking of CD4 and Fc gamma R by an anti-CD4 mAb (MAX.16H5) and by excess of goat anti-mouse Ig induced a biphasic increase of the free cytosolic Ca(2+)-concentration ([Ca2+]i) in the human monocytoid cell line U937 as measured by FURA-2 fluorescence. Fura-2 240-246 CD4 molecule Homo sapiens 50-53 1418854-7 1992 AII produced a concentration-related increase in calcium mobilization (fura-2 fluorescence) which was blocked by losartan (IC50 = 8.4 x 10(-8) mol/L) but not by PD123177 (10(-6) mol/L). Fura-2 71-77 angiotensinogen Rattus norvegicus 0-3 1393572-4 1992 Using Fura-2 loaded cells and dynamic ratio imaging, we have determined that arginine vasopressin (AVP) or V1- but not V2-receptor agonists, mobilise pituicyte intracellular Ca2+ ([Ca2+]i) in the absence of extracellular Ca2+. Fura-2 6-12 arginine vasopressin Rattus norvegicus 77-109 1527727-3 1992 Both thrombin and U46619 increased intracellular free calcium in fura-2 loaded, serum-deprived CHRF-288-11 cells with EC50 values of 0.07 +/- 0.01 U/ml and 194 +/- 27 nM, respectively (n = 4 each). Fura-2 65-71 coagulation factor II, thrombin Homo sapiens 5-13 1324922-1 1992 The regulation of extracellular Ca2+ entry into fura-2-loaded human platelets was examined following stimulation with thrombin. Fura-2 48-54 coagulation factor II, thrombin Homo sapiens 118-126 1505465-2 1992 [Ca2+]i was measured in cells loaded with the Ca(2+)-responsive fluorescent dye fura-2. Fura-2 80-86 carbonic anhydrase 2 Gallus gallus 1-4 1328896-3 1992 Changes in intracellular Ca2+ of single cells, measured using the fluorescent indicator dye fura-2, indicated that bradykinin produced a rapid, but transient, increase in intracellular calcium. Fura-2 92-98 kininogen 1 Homo sapiens 115-125 1380304-1 1992 In individual fura-2 loaded cells of rat pancreatic acini endothelin-1 (ET-1) (10-50 nM) induced sustained oscillations in [Ca2+]i. Fura-2 14-20 endothelin 1 Rattus norvegicus 58-70 1380304-1 1992 In individual fura-2 loaded cells of rat pancreatic acini endothelin-1 (ET-1) (10-50 nM) induced sustained oscillations in [Ca2+]i. Fura-2 14-20 endothelin 1 Rattus norvegicus 72-76 1514577-1 1992 In rat pancreatic and submandibular gland acini during exposure to carbachol, changes in the fluorescence emission intensity ratio (R) of acini loaded with mag-fura-2 resemble changes in cytosolic Ca2+ concentration (Ca2+i) in acini loaded with fura-2. Fura-2 160-166 carbonic anhydrase 2 Rattus norvegicus 217-220 1330055-2 1992 BK, but not IL-1 beta, caused a rapid, transient rise of intracellular Ca2+ concentration ([Ca2+]i), as assessed by recordings of fura-2 fluorescence in monolayers of prelabelled gingival fibroblasts. Fura-2 130-136 kininogen 1 Homo sapiens 0-2 1514647-5 1992 Stimulation of fura-2-loaded BPAEC with 10(-7) M alpha-thrombin produced a typical biphasic rise in [Ca2+]i. Fura-2 15-21 coagulation factor II, thrombin Bos taurus 55-63 1350327-6 1992 To study the mechanism that govern the spread of spikes in the dendrites of hippocampal pyramidal cells, we imaged Ca2+ entry with Fura-2 (ref. Fura-2 131-137 carbonic anhydrase 2 Homo sapiens 115-118 1322351-0 1992 Epidermal growth factor induces dose-dependent calcium oscillations in single fura-2-loaded hepatocytes. Fura-2 78-84 epidermal growth factor Homo sapiens 0-23 1322351-1 1992 Digital imaging fluorescence microscopy has been used to investigate epidermal growth factor-induced calcium responses of fura-2-loaded hepatocytes in primary culture at the single-cell level. Fura-2 122-128 epidermal growth factor Homo sapiens 69-92 1321817-1 1992 Cai in cultured human sweat gland epithelial monolayers was measured using Fura-2 fluorescence. Fura-2 75-81 carbonic anhydrase 1 Homo sapiens 0-3 1323639-4 1992 ET-1 dose-dependently (1-100 nmol/l) induced rapid and transient increases in [Ca2+]i in fura-2-labelled cells. Fura-2 89-95 endothelin 1 Homo sapiens 0-4 1616943-0 1992 Cytosolic calcium oscillations induced by hepatocyte growth factor (HGF) in single fura-2-loaded cultured hepatocytes: effects of extracellular calcium and protein kinase C. Hepatocyte growth factor (HGF) induced the periodic fluctuations of cytosolic calcium concentration ([Ca2+]i) in primary cultured rat hepatocytes, which were dependent on extracellular calcium. Fura-2 83-89 hepatocyte growth factor Rattus norvegicus 42-66 1616943-0 1992 Cytosolic calcium oscillations induced by hepatocyte growth factor (HGF) in single fura-2-loaded cultured hepatocytes: effects of extracellular calcium and protein kinase C. Hepatocyte growth factor (HGF) induced the periodic fluctuations of cytosolic calcium concentration ([Ca2+]i) in primary cultured rat hepatocytes, which were dependent on extracellular calcium. Fura-2 83-89 hepatocyte growth factor Rattus norvegicus 68-71 1616943-0 1992 Cytosolic calcium oscillations induced by hepatocyte growth factor (HGF) in single fura-2-loaded cultured hepatocytes: effects of extracellular calcium and protein kinase C. Hepatocyte growth factor (HGF) induced the periodic fluctuations of cytosolic calcium concentration ([Ca2+]i) in primary cultured rat hepatocytes, which were dependent on extracellular calcium. Fura-2 83-89 hepatocyte growth factor Rattus norvegicus 174-198 1590371-7 1992 Exposure of fura-2-loaded SqCC cells to PTHRP and PTH resulted in equivalent, dose-dependent transient increases in intracellular calcium [half-maximal effective concentration (EC50) = 0.08 nM]. Fura-2 12-18 parathyroid hormone Homo sapiens 40-43 1569926-1 1992 Human erythroleukemic (HEL) cells, loaded with fura-2, respond to neuropeptide Y (NPY) with a fast and transient increase in intracellular calcium. Fura-2 47-53 neuropeptide Y Homo sapiens 66-80 1590371-7 1992 Exposure of fura-2-loaded SqCC cells to PTHRP and PTH resulted in equivalent, dose-dependent transient increases in intracellular calcium [half-maximal effective concentration (EC50) = 0.08 nM]. Fura-2 12-18 parathyroid hormone like hormone Homo sapiens 40-45 1533221-2 1992 Addition of vasopressin caused a second, rapid quench of cellular fura-2, whereas the addition of thapsigargin had no effect. Fura-2 66-72 arginine vasopressin Homo sapiens 12-23 1533221-4 1992 When Mn2+ was removed from the medium of fura-2/AM-loaded cells after preincubation with Mn2+, vasopressin still caused quench of cellular fura-2. Fura-2 41-47 arginine vasopressin Homo sapiens 95-106 1533221-4 1992 When Mn2+ was removed from the medium of fura-2/AM-loaded cells after preincubation with Mn2+, vasopressin still caused quench of cellular fura-2. Fura-2 139-145 arginine vasopressin Homo sapiens 95-106 1560205-4 1992 In the present work we have confirmed the above hypothesis by measuring the [Ca2+]i of fura-2- or aequorin-labeled YAC-1 cells treated with cytolysin and low Na+ medium or amiloride analogs. Fura-2 87-93 perforin 1 (pore forming protein) Mus musculus 140-149 1575681-0 1992 Spiking in cytosolic calcium concentration in single fibrinogen-bound fura-2-loaded human platelets. Fura-2 70-76 fibrinogen beta chain Homo sapiens 53-63 1575681-1 1992 Fura-2-loaded human platelets were immobilized on a fibrinogen-coated surface and the cytosolic free calcium concentration ([Ca2+]i) was measured in single platelets by low-light-level video-ratio image-processing of the optical probe signal. Fura-2 0-6 fibrinogen beta chain Homo sapiens 52-62 1592099-1 1992 The effect of thrombin-activated platelets and their release products on the intracellular free calcium concentration ([Ca2+]i) of human polymorphonuclear leukocytes (PMNs) was studied by loading PMNs with a fluorescent indicator of calcium, fura-2. Fura-2 242-248 coagulation factor II, thrombin Homo sapiens 14-22 1569926-1 1992 Human erythroleukemic (HEL) cells, loaded with fura-2, respond to neuropeptide Y (NPY) with a fast and transient increase in intracellular calcium. Fura-2 47-53 neuropeptide Y Homo sapiens 82-85 1535691-4 1992 The pharmacological profiles of the binding (determined with [3H]8-OH-DPAT) and the calcium response (measured using Fura-2) were clearly of the 5-HT1A type. Fura-2 117-123 5-hydroxytryptamine receptor 1A Homo sapiens 145-151 1544231-6 1992 Furthermore, apoptosis could be induced in HL-60 cells under conditions of vastly reduced [Ca2+]i achieved by loading these cells with fura-2 in the presence of EGTA. Fura-2 135-141 carbonic anhydrase 2 Homo sapiens 91-94 1641824-2 1992 We used the fluorescent Ca2+ indicator, fura2, to measure both shape change as cell size and intracellular free Ca2+ ([Ca2+]i), in cultured human umbilical-vein endothelial cells (HUVEC). Fura-2 40-45 carbonic anhydrase 2 Homo sapiens 24-27 1641824-2 1992 We used the fluorescent Ca2+ indicator, fura2, to measure both shape change as cell size and intracellular free Ca2+ ([Ca2+]i), in cultured human umbilical-vein endothelial cells (HUVEC). Fura-2 40-45 carbonic anhydrase 2 Homo sapiens 112-115 1641824-2 1992 We used the fluorescent Ca2+ indicator, fura2, to measure both shape change as cell size and intracellular free Ca2+ ([Ca2+]i), in cultured human umbilical-vein endothelial cells (HUVEC). Fura-2 40-45 carbonic anhydrase 2 Homo sapiens 112-115 1593447-2 1992 Changes in intracellular ionized calcium [Ca2+]i induced by human growth hormone releasing factor (hGRF) were analysed by quantitative fluorescent microscopy using a dual-wavelength, ratiometric video imaging system and low light level charge-coupled device (CCD) camera visualizing Fura-2 in dispersed male rat anterior pituitary cells. Fura-2 283-289 growth hormone releasing hormone Homo sapiens 66-97 1737761-7 1992 Under the same conditions, in fura-2-loaded cells, thrombin-evoked Ca2+ entry (monitored by Mn2+ quench) was detectable after a delay of 1.4 s. This suggests that early thrombin-evoked Ca2+ entry occurs via small conductance channels, below the resolution of the patch clamp technique, or by an electroneutral pathway. Fura-2 30-36 coagulation factor II, thrombin Homo sapiens 51-59 1737761-7 1992 Under the same conditions, in fura-2-loaded cells, thrombin-evoked Ca2+ entry (monitored by Mn2+ quench) was detectable after a delay of 1.4 s. This suggests that early thrombin-evoked Ca2+ entry occurs via small conductance channels, below the resolution of the patch clamp technique, or by an electroneutral pathway. Fura-2 30-36 coagulation factor II, thrombin Homo sapiens 169-177 1593447-2 1992 Changes in intracellular ionized calcium [Ca2+]i induced by human growth hormone releasing factor (hGRF) were analysed by quantitative fluorescent microscopy using a dual-wavelength, ratiometric video imaging system and low light level charge-coupled device (CCD) camera visualizing Fura-2 in dispersed male rat anterior pituitary cells. Fura-2 283-289 growth hormone releasing hormone Homo sapiens 99-103 1620579-3 1992 In cells expressing the oncogene, but not in NIH fibroblasts not expressing the oncogene, bradykinin elicits calcium oscillations, which are detected by fura-2 fluorescence and amplified by a decrease of extracellular sodium activity. Fura-2 153-159 kininogen 1 Homo sapiens 90-100 1802362-7 1991 In the study with fura 2 excited at 340 nm, endothelin-1 abolished, from the tissue, the fluorescence signals that were coupled with spontaneous contraction. Fura-2 18-24 endothelin-1 Cavia porcellus 44-56 1371475-1 1992 We report in this paper using measurement of intracellular free Ca2+ with fura-2, that flufenamic acid and several related blockers of the 25 pS Ca(2+)-activated non-selective cation channel cause release of Ca2+ from an intracellular store other than the endoplasmic reticulum, possibly from mitochondria. Fura-2 74-80 carbonic anhydrase 2 Homo sapiens 64-67 1371475-1 1992 We report in this paper using measurement of intracellular free Ca2+ with fura-2, that flufenamic acid and several related blockers of the 25 pS Ca(2+)-activated non-selective cation channel cause release of Ca2+ from an intracellular store other than the endoplasmic reticulum, possibly from mitochondria. Fura-2 74-80 carbonic anhydrase 2 Homo sapiens 208-211 1309423-6 1992 Finally, thrombin stimulated an increase in cytosolic ionized calcium concentration ([Ca2+]i) in fura2-loaded platelets that was abolished by concentrations of EC which also blocked thrombin-induced aggregation. Fura-2 97-102 coagulation factor II, thrombin Homo sapiens 9-17 1309423-6 1992 Finally, thrombin stimulated an increase in cytosolic ionized calcium concentration ([Ca2+]i) in fura2-loaded platelets that was abolished by concentrations of EC which also blocked thrombin-induced aggregation. Fura-2 97-102 coagulation factor II, thrombin Homo sapiens 182-190 1333253-2 1992 With PAF, fMLP and LTB4 as agonists the Ca-transients measured with Fura-2 showed the same height of the intracellular Ca-signal but an external Ca-component in decreasing magnitude. Fura-2 68-74 PCNA clamp associated factor Homo sapiens 5-8 1333253-2 1992 With PAF, fMLP and LTB4 as agonists the Ca-transients measured with Fura-2 showed the same height of the intracellular Ca-signal but an external Ca-component in decreasing magnitude. Fura-2 68-74 formyl peptide receptor 1 Homo sapiens 10-14 1351266-1 1992 Spatiotemporal changes of the intracellular calcium ion (Ca2+) were recorded by digital ratio imaging of fura-2 in pyramidal neurons acutely isolated from the adult guinea-pig hippocampus. Fura-2 105-111 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 57-60 1329268-2 1992 Both AII and AVP specifically induce a transient increases in cytosolic free calcium independent of extracellular calcium or calcium channels activated by high potassium depolarization in VSM cells loaded with Fura-2. Fura-2 210-216 arginine vasopressin Rattus norvegicus 13-16 1329268-2 1992 Both AII and AVP specifically induce a transient increases in cytosolic free calcium independent of extracellular calcium or calcium channels activated by high potassium depolarization in VSM cells loaded with Fura-2. Fura-2 210-216 angiotensinogen Rattus norvegicus 5-8 1939188-0 1991 Oscillations of intracellular calcium induced by vasopressin in individual fura-2-loaded mesangial cells. Fura-2 75-81 arginine vasopressin Rattus norvegicus 49-60 1663589-4 1991 Measurements of changes in cytosolic Cai in Fura-2 loaded PMN demonstrate a transient Cai increase after stimulation with 0.1 microM fMLP and an additional sustained elevation of Cai levels in the presence of thimerosal. Fura-2 44-50 formyl peptide receptor 1 Homo sapiens 133-137 1719553-7 1991 When fura-2 was used as indicator for the cytosolic Ca2+, TRH induced a release from intracellular stores only once and had no effect on the intracellular Ca2+ concentration during further applications. Fura-2 5-11 thyrotropin releasing hormone Rattus norvegicus 58-61 1745976-4 1991 [Ca]i was assessed by use of fura-2, a calcium-sensitive fluorescent indicator. Fura-2 29-35 carbonic anhydrase 1 Bos taurus 1-5 1930148-3 1991 In the current study we have used the fluorescent indicator fura-2 to monitor the level of cytosolic Ca2+ ([Ca2+]i) in human skin fibroblasts. Fura-2 60-66 carbonic anhydrase 2 Homo sapiens 101-104 1953756-1 1991 The aim of the study was to investigate the relationship between thyrotropin-releasing hormone (TRH)-induced changes in intracellular free Ca2+ ([Ca2+]i), and influx of extracellular Ca2+ in Fura 2 loaded pituitary GH4C1 cells. Fura-2 191-197 thyrotropin releasing hormone Rattus norvegicus 65-94 1953756-1 1991 The aim of the study was to investigate the relationship between thyrotropin-releasing hormone (TRH)-induced changes in intracellular free Ca2+ ([Ca2+]i), and influx of extracellular Ca2+ in Fura 2 loaded pituitary GH4C1 cells. Fura-2 191-197 thyrotropin releasing hormone Rattus norvegicus 96-99 1953756-6 1991 In the presence of extracellular Mn2+, TRH stimulated quenching of Fura 2 fluorescence. Fura-2 67-73 thyrotropin releasing hormone Rattus norvegicus 39-42 1930148-3 1991 In the current study we have used the fluorescent indicator fura-2 to monitor the level of cytosolic Ca2+ ([Ca2+]i) in human skin fibroblasts. Fura-2 60-66 carbonic anhydrase 2 Homo sapiens 108-111 1936540-4 1991 Resting intracellular free Ca2+ levels ([Ca2+]i), measured using Fura-2, was determined to be 60 +/- 14.3 nM (mean +/- SE). Fura-2 65-71 carbonic anhydrase 2 Rattus norvegicus 27-30 1716460-5 1991 Exposure of fura-2-loaded acini to 10-1000 pM CCK-8 caused an immediate and dose-dependent increase in [Ca2+]i followed by a gradual decrease in [Ca2+]i. Fura-2 12-18 cholecystokinin Rattus norvegicus 46-49 1651654-9 1991 Measurements of cytosolic Ca2+ transients using fura-2 indicated that the ICa was responsible for triggering Ca2+ release from the sarcoplasmic reticulum. Fura-2 48-54 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 26-29 1651654-9 1991 Measurements of cytosolic Ca2+ transients using fura-2 indicated that the ICa was responsible for triggering Ca2+ release from the sarcoplasmic reticulum. Fura-2 48-54 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 109-112 1651495-2 1991 Measurements of intracellular Ca concentration [( Ca]i) using the fluorescent Ca indicator fura-2 showed that GABA potently inhibited Ca influx into the terminal elicited by high extracellular K concentration ([K]o). Fura-2 91-97 carbonic anhydrase 1 Homo sapiens 50-54 1651669-1 1991 The Ca(2+)-mobilizing action of bradykinin (BK) was investigated in ciliated human nasal epithelial (HNE) cells utilizing fura-2 fluorescence and microspectrofluorimetry. Fura-2 122-128 kininogen 1 Homo sapiens 32-42 1651669-1 1991 The Ca(2+)-mobilizing action of bradykinin (BK) was investigated in ciliated human nasal epithelial (HNE) cells utilizing fura-2 fluorescence and microspectrofluorimetry. Fura-2 122-128 kininogen 1 Homo sapiens 44-46 1742799-3 1991 Using the fluorescent indicator Fura-2, the concentration of intracellular free calcium ([Ca2+]i) in resting state cells was calculated to be 116 +/- 18.5 nM. Fura-2 32-38 carbonic anhydrase 2 Rattus norvegicus 90-93 1936540-4 1991 Resting intracellular free Ca2+ levels ([Ca2+]i), measured using Fura-2, was determined to be 60 +/- 14.3 nM (mean +/- SE). Fura-2 65-71 carbonic anhydrase 2 Rattus norvegicus 41-44 1864358-1 1991 In fura-2-loaded parotid acinar cells, 50-200 microM sphingosine induced an increase in cytosolic Ca2+ ([Ca2+]i). Fura-2 3-9 carbonic anhydrase 2 Rattus norvegicus 98-101 1650480-1 1991 Endothelin 1 causes a strong Ca2+ signal in C6 rat glioma cells as measured by fura-2 fluorescence. Fura-2 79-85 endothelin 1 Rattus norvegicus 0-12 1864358-1 1991 In fura-2-loaded parotid acinar cells, 50-200 microM sphingosine induced an increase in cytosolic Ca2+ ([Ca2+]i). Fura-2 3-9 carbonic anhydrase 2 Rattus norvegicus 105-108 1830482-6 1991 Concentration-dependent increases in intracellular free Ca2+ concentrations induced by I-BOP were observed in RASMC loaded with the calcium sensitive dye fura-2. Fura-2 154-160 opsin 1, short wave sensitive Rattus norvegicus 89-92 1945035-1 1991 Nerve growth factor (NGF) rapidly infused into the extracellular medium near cell bodies of cultured chick dorsal root ganglion neurons caused transient increases in intracellular calcium ions, which was detected by fura-2 fluorometry. Fura-2 216-222 nerve growth factor Gallus gallus 0-19 1945035-1 1991 Nerve growth factor (NGF) rapidly infused into the extracellular medium near cell bodies of cultured chick dorsal root ganglion neurons caused transient increases in intracellular calcium ions, which was detected by fura-2 fluorometry. Fura-2 216-222 nerve growth factor Gallus gallus 21-24 1653113-7 1991 Finally, experiments with Fura-2-loaded hepatocytes reveal that agents specifically releasing Ca2+ from non-mitochondrial stores (vasopressin and 2,5-di-tert-butyl-1-4-benzohydroquinone) are still able to increase the cytosolic Ca2+ concentration in FCCP-treated cells. Fura-2 26-32 carbonic anhydrase 2 Rattus norvegicus 94-97 1905229-1 1991 Dual wavelength digital imaging microscopy to detect fura-2 has been employed to characterize in normal bovine PRL-secreting cells the effects of TRH and dopamine on the intracellular ionized calcium concentration [( Ca2+]i). Fura-2 53-59 thyrotropin releasing hormone Bos taurus 146-149 2065474-2 1991 Video imaging of single, fura2-loaded vascular smooth muscle cells was used to examine the spatial and temporal alterations in calcium, Ca2+, in response to low levels of vasoconstrictor stimuli. Fura-2 25-30 carbonic anhydrase 2 Homo sapiens 136-139 1653853-11 1991 Calcium imaging experiments, using the Ca(2+)-sensitive probe, Fura-2, were performed to estimate the accumulation of Ca2+ in the presence of 10 mM-intracellular BAPTA. Fura-2 63-69 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 118-121 1850329-12 1991 Using the fluorescent calcium indicator fura-2, we demonstrated that thrombin increased the beating rate, diastolic calcium, and peak systolic calcium of spontaneously contracting cultured ventricular myocytes. Fura-2 40-46 coagulation factor II Rattus norvegicus 69-77 1649127-1 1991 We have used single-cell ratio imaging of Fura-2 loaded neutrophils to visualize release of cytosolic Ca2+ from an intracellular store in order to determine the location of this store and the relationship of release from it to oxidase activation. Fura-2 42-48 carbonic anhydrase 2 Homo sapiens 102-105 2026609-9 1991 The LHRH threshold and the quantal secretion process of metestrous gonadotropes was further studied by measuring cytosolic Ca2+ using fura-2 and digital imaging microscopy. Fura-2 134-140 gonadotropin releasing hormone 1 Homo sapiens 4-8 1881195-3 1991 Application of the method to mossy fiber tracts in hippocampal region CA3 and parallel fiber tracts in cerebellum resulted in distant presynaptic terminals well labelled with fura-2 without concomitant postsynaptic labelling, allowing optical measurements of calcium concentration in individual presynaptic terminals. Fura-2 175-181 carbonic anhydrase 3 Homo sapiens 70-73 2022753-6 1991 ET-1 dose-dependently induced increases in cytosolic free Ca2+ concentration in fura-2-loaded tumor cells, whose effect was completely abolished by chelating extracellular Ca2+ or by Ca(2+)-channel blocker. Fura-2 80-86 endothelin 1 Homo sapiens 0-4 1648132-11 1991 Video imaging of single-cell Fura-2 fluorescence from both intact cells and patch-clamped cells showed temporal correlation of the K+ current modulation and the Ca2+ transients in response to bradykinin stimulation. Fura-2 29-35 kininogen 1 Homo sapiens 192-202 1677314-1 1991 An increase in intracellular calcium concentration stimulated by anti-CD2 or CD3 antibodies has been measured with Fura-2 in P28 cells, a human CD4+ T cell clone. Fura-2 115-121 CD2 molecule Homo sapiens 70-73 1900792-9 1991 In single parietal cells double-labeled with fura-2 and BCECF, a temporal decrease in pHi preceded the rise in Cai after stimulation with nigericin. Fura-2 45-51 glucose-6-phosphate isomerase Homo sapiens 86-89 1881195-4 1991 Application of the method to CA1 pyramidal cells produced intracellular loading of apical dendrites with fura-2. Fura-2 105-111 carbonic anhydrase 1 Homo sapiens 29-32 2002040-8 1991 As evident from fura 2 fluorescence, bradykinin leads to a transient increase of intracellular calcium both in the presence and absence of extracellular calcium. Fura-2 16-22 kininogen 1 Homo sapiens 37-47 2067597-2 1991 In this paper, changes in intracellular Ca2+ concentration ([Ca2+]1) in response to CCK peptides were measured in single cultured rat striatal neuron by fura-2 fluorometry. Fura-2 153-159 cholecystokinin Rattus norvegicus 84-87 1900287-5 1991 A similar effect of thrombin was seen on video imaging of Fura-2-loaded cell monolayers. Fura-2 58-64 coagulation factor II, thrombin Homo sapiens 20-28 1900390-9 1991 Measurements of intracellular Ca2+ concentrations with fura-2 show that intracellular Ca2+ rises in hyposmotic solutions and that this rise does not occur in absence of extracellular Ca2+. Fura-2 55-61 carbonic anhydrase 2 Oryctolagus cuniculus 30-33 1900390-9 1991 Measurements of intracellular Ca2+ concentrations with fura-2 show that intracellular Ca2+ rises in hyposmotic solutions and that this rise does not occur in absence of extracellular Ca2+. Fura-2 55-61 carbonic anhydrase 2 Oryctolagus cuniculus 86-89 1900390-9 1991 Measurements of intracellular Ca2+ concentrations with fura-2 show that intracellular Ca2+ rises in hyposmotic solutions and that this rise does not occur in absence of extracellular Ca2+. Fura-2 55-61 carbonic anhydrase 2 Oryctolagus cuniculus 86-89 2003581-12 1991 Simultaneous measurement of membrane K+ current and fura-2 fluorescence in the same cell demonstrated a correlation between the rise in [Ca2+]c and increase in K+ current. Fura-2 52-58 carbonic anhydrase 2 Homo sapiens 137-140 1742874-5 1991 We used fura 2 (pentapotassium salt) to confirm that the ryanodine we used was capable of abolishing Ca2+ release from the sarcoplasmic reticulum during the period in which it was present. Fura-2 8-14 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 101-104 1837489-1 1991 Using fura-2 loaded neural tumour cells, SK-N-SH, we demonstrate that receptor-mediated activation of phosphoinositide hydrolysis not only causes the release of Ca2+ from intracellular stores but also causes a concomitant influx of extracellular Ca2+. Fura-2 6-12 carbonic anhydrase 2 Homo sapiens 161-164 1996698-8 1991 These findings, together with our previously described results using fura-2, suggest that oxidant stress initially inhibits bradykinin-stimulated Ca2+ influx and later inhibits stimulated Ca2+ efflux. Fura-2 69-75 kininogen 1 Bos taurus 124-134 1705122-6 1991 Stimulation of fura-2 loaded cells with ET-1 produced an unusual timecourse of response which rose slowly to a maximum which was sustained. Fura-2 15-21 endothelin 1 Bos taurus 40-44 2015414-1 1991 Using microfluorometric analysis in individual, fura-2 loaded cells, we found that thrombin (0.1-10 U/ml) caused a dose-dependent (EC50 approximately 0.5 U/ml), rapid (within seconds), transient increase in cytoplasmic Ca2+ in the osteoblastic cell line MC3T3-El. Fura-2 48-54 coagulation factor II, thrombin Homo sapiens 83-91 2059991-0 1991 Ca2+ oscillations induced by histamine H1 receptor stimulation in HeLa cells: Fura-2 and patch clamp analysis. Fura-2 78-84 histamine receptor H1 Homo sapiens 29-50 1849803-1 1991 Through assessment of the changes in the intracellular free-calcium concentration ([Ca2+]i), which was measured using the calcium sensitive dye, fura-2, the character of the neurotensin (NT) binding sites which appeared transiently during the early ontogenetic stage in the rat cerebral cortex was analyzed in primary cultures of cerebral cortex cells from neonatal rats. Fura-2 145-151 neurotensin Rattus norvegicus 174-185 19215446-1 1991 Abstract The effect of thyrotrophin-releasing hormone (TRH) on intracellular free Ca(2+) concentration, [Ca(2+))i, was investigated with the fluorescent dye fura-2 in cell suspensions obtained from 13 human growth hormone-secreting adenomas and 6 adrenocorticotrophin-secreting adenomas. Fura-2 157-163 thyrotropin releasing hormone Homo sapiens 55-58 1725355-6 1991 ET-1 caused a prompt rise in the fura-2-associated fluorescence intensity in the individual A-10 cell and it fell to a lower plateau level that was still higher than the baseline value. Fura-2 33-39 endothelin 1 Rattus norvegicus 0-4 1988097-1 1991 Digitized video-intensified fluorescence microscopy with the Ca2(+)-sensitive fluorescent dye fura-2 was used to measure cytosolic free Ca2+ [( Ca2+]f) in HT-29 human colon cancer cells. Fura-2 94-100 carbonic anhydrase 2 Homo sapiens 61-64 1988097-1 1991 Digitized video-intensified fluorescence microscopy with the Ca2(+)-sensitive fluorescent dye fura-2 was used to measure cytosolic free Ca2+ [( Ca2+]f) in HT-29 human colon cancer cells. Fura-2 94-100 carbonic anhydrase 2 Homo sapiens 136-139 1988097-1 1991 Digitized video-intensified fluorescence microscopy with the Ca2(+)-sensitive fluorescent dye fura-2 was used to measure cytosolic free Ca2+ [( Ca2+]f) in HT-29 human colon cancer cells. Fura-2 94-100 carbonic anhydrase 2 Homo sapiens 136-139 1892734-1 1991 Stimulation of suspensions of fura-2-loaded human neutrophils with ATP resulted in an elevation in cytosolic free calcium concentration ([Ca2+]i) from a basal value of 0.1 microM to a transient peak of 1 microM. Fura-2 30-36 carbonic anhydrase 2 Homo sapiens 138-141 1725305-1 1991 The present study was undertaken to examine the interaction between endothelin-1 (ET-1)-induced Ca2+ and Na+ kinetics in cultured rat vascular smooth muscle cells (VSMCs) by measuring cytosolic free Ca2+ ([Ca2+]i) and intracellular Na+ ([Na+]i) using intracellular fluorescent dyes (fura-2 and SBFI, respectively). Fura-2 283-289 endothelin 1 Rattus norvegicus 68-80 1725305-1 1991 The present study was undertaken to examine the interaction between endothelin-1 (ET-1)-induced Ca2+ and Na+ kinetics in cultured rat vascular smooth muscle cells (VSMCs) by measuring cytosolic free Ca2+ ([Ca2+]i) and intracellular Na+ ([Na+]i) using intracellular fluorescent dyes (fura-2 and SBFI, respectively). Fura-2 283-289 endothelin 1 Rattus norvegicus 82-86 1984207-1 1991 The changes in intracellular free calcium concentration ([Ca2+]i) induced by oxytocin in single cells of cultured human puerperal myometrium were measured with the calcium-sensitive fluorescent dye fura 2 in a digital imaging fluorescence microscopic system. Fura-2 198-204 oxytocin/neurophysin I prepropeptide Homo sapiens 77-85 1942669-1 1991 We describe a new system to measure changes in cytosolic free Ca concentration ([Ca2+]i) and pH (pHi) simultaneously using the dyes fura-2 and BCECF, and a random access camera with computer-operated synchronously-changing excitation and emission filters. Fura-2 132-138 glucose-6-phosphate isomerase Rattus norvegicus 97-100 1899461-1 1991 Confluent monolayers of human umbilical vein endothelial cells subcultured on glass coverslips were loaded with the fluorescent Ca2+ indicator, fura-2. Fura-2 144-150 carbonic anhydrase 2 Homo sapiens 128-131 20504745-3 1991 A saturating concentration of ET-2 (100 nM) increased intracellular free calcium (measured by Fura-2 fluorescence) from a resting level of ? Fura-2 94-100 endothelin 2 Mus musculus 30-34 1725410-5 1991 By fura-2 fluorescence method, transient increase and oscillation of intracellular Ca2+ level were observed in the cultured myofibroblasts following the application of ET-1 or endothelin-3 (ET-3). Fura-2 3-9 endothelin 1 Rattus norvegicus 168-172 1725410-5 1991 By fura-2 fluorescence method, transient increase and oscillation of intracellular Ca2+ level were observed in the cultured myofibroblasts following the application of ET-1 or endothelin-3 (ET-3). Fura-2 3-9 endothelin 3 Rattus norvegicus 176-188 1725410-5 1991 By fura-2 fluorescence method, transient increase and oscillation of intracellular Ca2+ level were observed in the cultured myofibroblasts following the application of ET-1 or endothelin-3 (ET-3). Fura-2 3-9 endothelin 3 Rattus norvegicus 190-194 2260972-6 1990 The present study was undertaken to determine the effect of HgCl2 on ANG II-induced [Ca++]i increase using Fura-2. Fura-2 107-113 angiotensinogen Rattus norvegicus 69-75 2260994-5 1990 Pretreatment with the phospholipase A2 inhibitor dexamethasone potently inhibited both ET-1 and PAF-induced elevation of cytosolic free Ca2+ concentrations [( Ca2+]i) in fura-2-loaded VSMC. Fura-2 170-176 phospholipase A2 group IB Rattus norvegicus 22-38 2260994-5 1990 Pretreatment with the phospholipase A2 inhibitor dexamethasone potently inhibited both ET-1 and PAF-induced elevation of cytosolic free Ca2+ concentrations [( Ca2+]i) in fura-2-loaded VSMC. Fura-2 170-176 endothelin 1 Rattus norvegicus 87-91 2175792-5 1990 Octimibate also inhibited the rise in intracellular Ca++ caused by thrombin, as measured using Fura-2-loaded platelets, which is consistent with octimibate"s ability to elevate platelet cAMP levels. Fura-2 95-101 coagulation factor II, thrombin Homo sapiens 67-75 2268367-4 1990 We observed in fura-2 loaded cells that pretreatment with manoalide blocked fMLP-induced increases in cytosolic free-calcium (IC50 approximately 0.15 microM). Fura-2 15-21 formyl peptide receptor 1 Homo sapiens 76-80 2171931-7 1990 When the effect of erythropoietin and Me2SO on the intracellular Ca2+ level was examined using fura 2, no acute change was observed. Fura-2 95-101 erythropoietin Mus musculus 19-33 1701016-9 1990 Ca2+ mobilization, measured by fura-2 fluorescence, was coincident with the increase in tyrosine phosphorylation in response to angiotensin II or thapsigargin. Fura-2 31-37 angiotensinogen Rattus norvegicus 128-142 1704288-7 1990 Depolarization-induced [Ca2+]i transients assessed using fura-2 showed that the inhibitory effects of nitrendipine and NPY were additive. Fura-2 57-63 neuropeptide Y Rattus norvegicus 119-122 1963319-1 1990 Preincubation of Fura 2-loaded rat myometrial cells with H-8, an inhibitor of protein kinase A, for 1 h reversed the inhibitory effects of 8-(4-chlorophenylthio)-cAMP (CPTcAMP) on the oxytocin-stimulated increase in (Ca2+)i (intracellular free calcium), with an EC50 of 47 microM. Fura-2 17-23 protein kinase cAMP-activated catalytic subunit alpha Rattus norvegicus 78-103 2145278-1 1990 Stimulation of macrophages with platelet-activating factor (PAF) elicits an increase of intracellular calcium concentration, Ca2+i, which was monitored here at the single cell level with the calcium-sensitive dye Fura-2. Fura-2 213-219 PCNA clamp associated factor Homo sapiens 32-58 2145278-1 1990 Stimulation of macrophages with platelet-activating factor (PAF) elicits an increase of intracellular calcium concentration, Ca2+i, which was monitored here at the single cell level with the calcium-sensitive dye Fura-2. Fura-2 213-219 PCNA clamp associated factor Homo sapiens 60-63 2170382-3 1990 In the presence of extracellular Mn2+, the Ca2(+)-mobilizing hormone vasopressin produced a severalfold stimulation of the basal rate of fura-2 fluorescence quenching as a result of Mn2+ influx; this effect was blocked by the presence of Ni2+ in the incubation medium. Fura-2 137-143 arginine vasopressin Rattus norvegicus 69-80 2168414-3 1990 Although NaF induced the mobilization of Ca2+ from intracellular storage sites in fura2-loaded platelets, it failed to raise pHi as determined from the fluorescence of 2,7-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein-loaded platelets. Fura-2 82-87 C-X-C motif chemokine ligand 8 Homo sapiens 9-12 2172374-2 1990 Endothelin-1 was found to increase the intracellular Ca2+ level, either in the presence or absence of extracellular Ca2+, in endothelial cells pretreated with antiserum by the fura-2 fluorescence technique. Fura-2 176-182 endothelin 1 Homo sapiens 0-12 1963337-1 1990 Bradykinin triggered intracellular Ca mobilizations and ionic conductance changes were studied in the neuroblastoma x glioma hybrid cell line NG108-15 using Ca-sensitive fluorescent indicator fura-2 under patch pipette whole cell voltage clamp condition. Fura-2 192-198 kininogen 1 Homo sapiens 0-10 2393384-1 1990 Cytoplasmic Ca2+ mobilization in human platelets triggered by a CD 9 monoclonal antibody, TP82, was monitored by the Ca2(+)-sensitive photoprotein, aequorin and Ca2(+)-sensitive fluorophores, fura 2 and quin 2. Fura-2 192-198 CD9 molecule Homo sapiens 64-68 2168700-4 1990 In the presence of Mn2+, an ion that quenches fura-2 fluorescence, fura-2-loaded platelets were stimulated with thrombin or ADP. Fura-2 46-52 coagulation factor II, thrombin Homo sapiens 112-120 2168700-4 1990 In the presence of Mn2+, an ion that quenches fura-2 fluorescence, fura-2-loaded platelets were stimulated with thrombin or ADP. Fura-2 67-73 coagulation factor II, thrombin Homo sapiens 112-120 2165364-2 1990 The unique properties of the CCD detector were applied to an analysis of cell shortening and the Ca2+ transient from fluorescence images of fura-2-loaded [corrected] cardiomyocytes. Fura-2 140-146 carbonic anhydrase 2 Homo sapiens 97-100 2165364-8 1990 Raising the intracellular load of fura-2 had little effect on the rising phase of Ca2+ or the extent of shortening but extended the duration of the Ca2+ transient and contraction. Fura-2 34-40 carbonic anhydrase 2 Homo sapiens 148-151 2162247-7 1990 Fura2 experiments also demonstrated that, in addition to omega CTx-sensitive calcium channels, SCC cell lines also expressed omega CTx-insensitive calcium channels, which were antagonized by nitrendipine and verapamil. Fura-2 0-5 serpin family B member 3 Homo sapiens 95-98 2388255-9 1990 Because fura-2 was found to decrease its affinity for Ca as pH decreased below 6.7, corrections were applied to experiments in which large pHi changes were observed. Fura-2 8-14 glucose-6-phosphate isomerase Oryctolagus cuniculus 139-142 2161346-5 1990 Using the intracellular Ca2(+)-indicator fura-2, we observed that the addition of thrombin to the glioma cells generated a Ca2(+)-signal which was inhibited by pretreatment of the cells with hirudin or with 1 mM neomycin. Fura-2 41-47 coagulation factor II Rattus norvegicus 82-90 2113061-1 1990 Digital imaging fluorescence microscopy of fura-2-loaded hepatocytes in primary culture has been used to examine the changes of cytosolic free Ca2+ ([Ca2+]i) in response to receptor activation by alpha 1-adrenergic agonists and vasopressin at the subcellular level. Fura-2 43-49 arginine vasopressin Homo sapiens 228-239 2383431-4 1990 At the concentrations tested nitric oxide effectively blocked thrombin-stimulated mobilization of cytosolic-free calcium in Fura 2-loaded platelets. Fura-2 124-130 coagulation factor II, thrombin Homo sapiens 62-70 2161879-8 1990 Peak levels of intracellular Ca2+ ([Ca]i) at 5 to 10 s, and levels of [Ca]i 120 s after FMLP in Fura 2-loaded cells were significantly lower in cells treated with lidocaine, findings that could be explained by enhanced 45Ca2+ efflux from neutrophils. Fura-2 96-102 formyl peptide receptor 1 Homo sapiens 88-92 1973064-2 1990 [Ca2+]i was measured with Fura-2 using the ratiometric method. Fura-2 26-32 carbonic anhydrase 2 Homo sapiens 1-4 2338678-8 1990 Thrombin 0.5 U/ml added to platelets in Ca-free media caused a multiphasic rise in (Ca)i with Fura 2. Fura-2 94-100 coagulation factor II, thrombin Homo sapiens 0-8 2318379-0 1990 Effect of buffer systems and pHi on the measurement of [Ca2+]i with fura 2. Fura-2 68-74 glucose-6-phosphate isomerase Rattus norvegicus 29-32 2318379-7 1990 It was found that measurements of [Ca2+]i with fura 2 were influenced by shifts in pHi that occur when cells are incubated in either HEPES-buffered or CO2/HCO3- media of differing pHo values. Fura-2 47-53 glucose-6-phosphate isomerase Rattus norvegicus 83-86 2158310-1 1990 In fura-2-loaded A10 vascular smooth-muscle cells, 1 nM-vasopressin and 200 nM-endothelin evoked a rapid transient rise in intracellular free Ca2+ concentration [( Ca2+]i), which was then followed by a maintained elevation of [Ca2+]i. Fura-2 3-9 immunoglobulin kappa variable 6D-21 (non-functional) Homo sapiens 17-20 2158310-1 1990 In fura-2-loaded A10 vascular smooth-muscle cells, 1 nM-vasopressin and 200 nM-endothelin evoked a rapid transient rise in intracellular free Ca2+ concentration [( Ca2+]i), which was then followed by a maintained elevation of [Ca2+]i. Fura-2 3-9 arginine vasopressin Homo sapiens 56-67 2158310-3 1990 When extracellular Ca2+ was replaced with 1 mM-Mn2+ (Mn2+ quenches fura-2 fluorescence), both endothelin and vasopressin evoked an Mn2+ quench of the fluorescence from the intracellularly trapped fura-2, even in the presence of 5 microM-nifedipine. Fura-2 67-73 arginine vasopressin Homo sapiens 109-120 2158310-3 1990 When extracellular Ca2+ was replaced with 1 mM-Mn2+ (Mn2+ quenches fura-2 fluorescence), both endothelin and vasopressin evoked an Mn2+ quench of the fluorescence from the intracellularly trapped fura-2, even in the presence of 5 microM-nifedipine. Fura-2 196-202 arginine vasopressin Homo sapiens 109-120 2155611-1 1990 Vasoactive intestinal contractor peptide (VIC), a novel member of the endothelin family, stimulated a rapid increase in the intracellular Ca2+ concentration in fura-2-loaded Swiss 3T3 cells. Fura-2 160-166 endothelin 2 Mus musculus 0-40 2306515-1 1990 We found that a monoclonal antibody (MoAb) to CD9 antigen, PMA2, induced a rise in cytosolic free calcium concentration ([Ca2+]i) in fura-2-loaded platelets, and we examined whether this response was due to direct action of PMA2 on CD9 antigen. Fura-2 133-139 CD9 molecule Homo sapiens 46-49 2155611-1 1990 Vasoactive intestinal contractor peptide (VIC), a novel member of the endothelin family, stimulated a rapid increase in the intracellular Ca2+ concentration in fura-2-loaded Swiss 3T3 cells. Fura-2 160-166 endothelin 2 Mus musculus 42-45 2155611-1 1990 Vasoactive intestinal contractor peptide (VIC), a novel member of the endothelin family, stimulated a rapid increase in the intracellular Ca2+ concentration in fura-2-loaded Swiss 3T3 cells. Fura-2 160-166 endothelin 1 Mus musculus 70-80 2295684-7 1990 We have used microspectrofluorometry to measure [Ca2+]i in single fura-2-loaded human neutrophils as they moved on poly-D-lysine-coated glass in the presence of serum. Fura-2 66-72 carbonic anhydrase 2 Homo sapiens 49-52 2154452-1 1990 The effects of membrane potential on resting and bradykinin-stimulated changes in [Ca2+]i were measured in fura-2 loaded cultured endothelial cells from bovine atria by spectrofluorimetry. Fura-2 107-113 kininogen 1 Bos taurus 49-59 2306207-0 1990 ADP evokes biphasic Ca2+ influx in fura-2-loaded human platelets. Fura-2 35-41 carbonic anhydrase 2 Homo sapiens 20-23 2306207-2 1990 Stopped-flow fluorimetric studies at 37 degrees C have shown that ADP, at optimal concentrations, can evoke Ca2+ or Mn2+ influx in fura-2-loaded human platelets without measurable delay. Fura-2 131-137 carbonic anhydrase 2 Homo sapiens 108-111 1688510-1 1990 The tachykinin family of neuropeptides, including substance P and neurokinins A and B, induce a transient increase in intracellular free calcium concentration in human small cell lung carcinoma (SCLC) cells, as measured with a calcium indicator fura-2. Fura-2 245-251 tachykinin precursor 1 Homo sapiens 50-61 1688510-1 1990 The tachykinin family of neuropeptides, including substance P and neurokinins A and B, induce a transient increase in intracellular free calcium concentration in human small cell lung carcinoma (SCLC) cells, as measured with a calcium indicator fura-2. Fura-2 245-251 tachykinin precursor 1 Homo sapiens 66-85 2328733-1 1990 A digital imaging microscope and fluorescent Ca(2+)-sensitive probe (Fura 2) were used to study the spatial location and time course of increases in free intracellular calcium (Cai) induced by platelet-derived growth factor (PDGF). Fura-2 69-75 protein disulfide isomerase associated 4 Mus musculus 168-175 2328733-1 1990 A digital imaging microscope and fluorescent Ca(2+)-sensitive probe (Fura 2) were used to study the spatial location and time course of increases in free intracellular calcium (Cai) induced by platelet-derived growth factor (PDGF). Fura-2 69-75 protein disulfide isomerase associated 4 Mus musculus 177-180 2154211-4 1990 In addition, PAF from 10(-6)-10(-8)M when added to Fura-2 containing B cells induced a rapid and significant rise of calcium within the cell, with lyso-PAF having no effect. Fura-2 51-57 PCNA clamp associated factor Homo sapiens 13-16 2153577-3 1990 Both NPY and the specific Y1 receptor ligand, [Leu31,Pro34]-NPY, caused a rapid and transient increase in the concentration of free calcium in the cytoplasm as measured by the fluorescent probe, Fura-2. Fura-2 195-201 neuropeptide Y Homo sapiens 5-8 2153577-3 1990 Both NPY and the specific Y1 receptor ligand, [Leu31,Pro34]-NPY, caused a rapid and transient increase in the concentration of free calcium in the cytoplasm as measured by the fluorescent probe, Fura-2. Fura-2 195-201 neuropeptide Y Homo sapiens 60-63 2294097-1 1990 The effects of oxidant stress and inhibition of glutathione reductase on the bradykinin-stimulated changes in cytosolic free Ca2+ concentration ([Ca2+]i) of calf pulmonary artery endothelial cells were determined using the intracellular fluorescent probe, fura-2. Fura-2 256-262 kininogen 1 Bos taurus 77-87 2298824-8 1990 The effect of EGF on the intracellular mobilization of Ca2+ and the formation of inositol phosphates was studied by using the calcium-sensitive fluorescent indicator fura 2 and [3H]inositol. Fura-2 166-172 epidermal growth factor Mus musculus 14-17 33788969-5 2021 MrgprX1 receptor signaling was monitored by Fura-2-based Ca2+ imaging using PNEs and membrane potential responses were measured using FluoVoltTM . Fura-2 44-50 MAS related GPR family member X1 Homo sapiens 0-7 1330027-12 1992 In two experiments involving the injection of larger amounts of fura-2, reductions in SR Ca2+ release were observed; however, we were unable to decide whether these reductions were due to [fura-2T] or to some nonspecific effect of the injection itself. Fura-2 64-70 carbonic anhydrase 2 Homo sapiens 89-92 7714589-2 1995 Individual immortalized gonadotropin-releasing hormone (GnRH)-secreting hypothalamic (GT1-7) neurons in semiconfluent cultures showed spontaneous oscillations in intracellular Ca2+ concentration ([Ca2+]i) as measured by video fluorescence microscopy and fura-2. Fura-2 254-260 gonadotropin releasing hormone 1 Homo sapiens 24-54 7714589-2 1995 Individual immortalized gonadotropin-releasing hormone (GnRH)-secreting hypothalamic (GT1-7) neurons in semiconfluent cultures showed spontaneous oscillations in intracellular Ca2+ concentration ([Ca2+]i) as measured by video fluorescence microscopy and fura-2. Fura-2 254-260 beta-1,4-galactosyltransferase 1 Homo sapiens 86-91 1330027-1 1992 Experiments were carried out to test the hypothesis that mM concentrations of fura-2, a high-affinity Ca2+ buffer, inhibit the release of Ca2+ from the sarcoplasmic reticulum (SR) of skeletal muscle fibers. Fura-2 78-84 carbonic anhydrase 2 Homo sapiens 102-105 1330027-1 1992 Experiments were carried out to test the hypothesis that mM concentrations of fura-2, a high-affinity Ca2+ buffer, inhibit the release of Ca2+ from the sarcoplasmic reticulum (SR) of skeletal muscle fibers. Fura-2 78-84 carbonic anhydrase 2 Homo sapiens 138-141 1330027-4 1992 At [fura-2T] = 2-3 mM, the amplitude and half-width of delta [Ca2+] were reduced to approximately 25% of the values measured at [fura-2T] less than 0.15 mM, whereas the amount and rate of SR Ca2+ release were enhanced by approximately 50% (n = 5; 16 degrees C). Fura-2 4-10 carbonic anhydrase 2 Homo sapiens 62-65 1330027-4 1992 At [fura-2T] = 2-3 mM, the amplitude and half-width of delta [Ca2+] were reduced to approximately 25% of the values measured at [fura-2T] less than 0.15 mM, whereas the amount and rate of SR Ca2+ release were enhanced by approximately 50% (n = 5; 16 degrees C). Fura-2 4-10 carbonic anhydrase 2 Homo sapiens 191-194 1330027-4 1992 At [fura-2T] = 2-3 mM, the amplitude and half-width of delta [Ca2+] were reduced to approximately 25% of the values measured at [fura-2T] less than 0.15 mM, whereas the amount and rate of SR Ca2+ release were enhanced by approximately 50% (n = 5; 16 degrees C). Fura-2 129-135 carbonic anhydrase 2 Homo sapiens 62-65 34607973-11 2021 We evaluated the accumulation of VEGF by fura-2/AM fluorescence. Fura-2 41-47 vascular endothelial growth factor A Mus musculus 33-37 34950652-5 2021 The effects of hypoxia and p65 expression on cell proliferation, invasion, apoptosis, (Ca2+)i, PPARgamma, and TRPC1/6 expression were determined using Cell Counting Kit-8 (CCK-8), Transwell, Annexin V/PI, Fura-2/AM, and western blotting, respectively. Fura-2 205-211 RELA proto-oncogene, NF-kB subunit Homo sapiens 27-30 34737238-6 2021 Using Fura-2 based Ca2+ imaging, we observed reproducible intracellular Ca2+ responses induced by Pam3CSK4 and Pam2CSK4 (TLR2-specific agonists) in TG neurons of adult wild-type (WT) mice. Fura-2 6-12 toll-like receptor 2 Mus musculus 121-125 2686633-4 1989 Intracellular calcium concentration [( Ca]i) was measured in single H7CM cells using the fluorescent calcium indicator fura-2. Fura-2 119-125 carbonic anhydrase 1 Homo sapiens 39-43 35302175-4 2022 The effect of Sig-1R overexpression on cell proliferation detected by EdU staining, cell cycle progression by propidium iodide (PI), apoptosis by Annexin V-APC/PI, mitochondrial membrane potential by Mitolite Red and cytoplasmic Ca2+ levelsby Fura-2/AM in islet beta-cells were measured by flow cytometry. Fura-2 243-249 sigma non-opioid intracellular receptor 1 Mus musculus 14-20 2558572-2 1989 ET specifically binds to a single class of high-affinity receptors in MC3T3-E1 cells and induces phospholipase C activation with the production of two second messengers, inositol trisphosphate and 1,2-diacylglycerol, and a biphasic increase in intracellular free Ca2+ concentration ([Ca2+]i), which consists of an initial transient increase and an ensuing sustained plateau, as measured with a fluorescent indicator, fura-2. Fura-2 417-423 endothelin 1 Mus musculus 0-2 2531141-1 1989 Fura-2 fluorescence in single rat basophilic leukemia cells was monitored to study the rise in intracellular free ionized calcium ([Ca2+]i) produced by aggregation of immunoglobulin E receptors. Fura-2 0-6 carbonic anhydrase 2 Rattus norvegicus 132-135 34995919-10 2022 However, STIM1 knocking down reduced NCX1 activity recorded by either patch-clamp electrophysiology or Fura-2 single-cell microfluorimetry. Fura-2 103-109 stromal interaction molecule 1 Rattus norvegicus 9-14 2514601-2 1989 In this study, [Ca2+]i was determined with the fluorescence indicator fura-2 at the single-cell level with a fluorescent microscope-imaging system. Fura-2 70-76 carbonic anhydrase 2 Homo sapiens 16-19 2808386-5 1989 IGF-I also increases [Ca2+]i, measured using fura-2, a fluorescent Ca2+ indicator; the IGF-I-induced [Ca2+]i response occurs immediately, reaches a maximum within 1 min, and then slowly declines. Fura-2 45-51 insulin like growth factor 1 Homo sapiens 0-5 2684691-1 1989 Effects on [Ca2+]i levels of endothelin-l (ET) and vasoactive intestinal contractor peptide (VIC), which is a novel member of the endothelin family, were examined in fura 2-loaded neuroblastoma NG108-15 cells. Fura-2 166-172 endothelin 2 Mus musculus 93-96 2808386-5 1989 IGF-I also increases [Ca2+]i, measured using fura-2, a fluorescent Ca2+ indicator; the IGF-I-induced [Ca2+]i response occurs immediately, reaches a maximum within 1 min, and then slowly declines. Fura-2 45-51 insulin like growth factor 1 Homo sapiens 87-92 2777805-2 1989 We studied thrombin-induced changes in cytosolic calcium concentration (Ca2+i) using microfluorometry of fura-2-loaded single cells, cell topography (scanning electron microscopy), and cytoskeleton (rhodamine phalloidin) in endothelial cells. Fura-2 105-111 coagulation factor II, thrombin Homo sapiens 11-19 2573029-4 1989 (EMBO J., 8, 159-166, 1989), EGF (10(-9)-10(-8) M) was found to induce marked increases of both [Ca2+]i and plasma membrane potential (investigated by the fura-2 and bis-oxonol techniques) which, in their initial phase, were only marginally dependent on the presence of Ca2+ in the incubation medium. Fura-2 155-161 epidermal growth factor Homo sapiens 29-32 2793847-0 1989 Characterization of cytosolic calcium oscillations induced by phenylephrine and vasopressin in single fura-2-loaded hepatocytes. Fura-2 102-108 arginine vasopressin Homo sapiens 80-91 2782436-4 1989 The effect of membrane depolarization with high K+ buffer on the bradykinin-stimulated changes in cytosolic Ca2+ was determined using the fluorescent Ca2+ indicator, fura-2. Fura-2 166-172 kininogen 1 Bos taurus 65-75 2508482-6 1989 Parathyroid hormone (PTH) secretion was inhibited by ATP gamma S but not by those nucleotides that were without effect on [Ca2+]i. Loading cells with 1,2-bis-(2-aminophenoxy)ethane-N,N,N",N"-tetraacetic acid and fura-2 blocked cytosolic Ca2+ transients elicited by ATP gamma S but did not block the inhibitory effects of ATP gamma S on PTH secretion. Fura-2 212-218 parathyroid hormone Bos taurus 0-19 2508482-6 1989 Parathyroid hormone (PTH) secretion was inhibited by ATP gamma S but not by those nucleotides that were without effect on [Ca2+]i. Loading cells with 1,2-bis-(2-aminophenoxy)ethane-N,N,N",N"-tetraacetic acid and fura-2 blocked cytosolic Ca2+ transients elicited by ATP gamma S but did not block the inhibitory effects of ATP gamma S on PTH secretion. Fura-2 212-218 parathyroid hormone Bos taurus 21-24 2546937-5 1989 Fura-2 fluorescence was used to compare the profiles for BK-stimulated changes in cytosolic Ca2+, Sr2+, and Ba2+ (Ca2+i, Ba2+i, and Sr2+i). Fura-2 0-6 kininogen 1 Homo sapiens 57-59 2550000-1 1989 The ability of cholecystokinin (CCK) to elevate intracellular Ca2+ levels in small cell lung cancer cells was investigated using the fluorescent Ca2+ indicator Fura 2. Fura-2 160-166 cholecystokinin Homo sapiens 15-30 2550000-1 1989 The ability of cholecystokinin (CCK) to elevate intracellular Ca2+ levels in small cell lung cancer cells was investigated using the fluorescent Ca2+ indicator Fura 2. Fura-2 160-166 cholecystokinin Homo sapiens 32-35 2775282-5 1989 It is concluded that [Ca2+]i and pHi could be measured simultaneously in isolated myocyte using dual-loading of fura-2 and BCECF. Fura-2 112-118 glucose-6-phosphate isomerase Rattus norvegicus 33-36 2545786-3 1989 PAF was found to elicit an increase in the cytoplasmic level of free Ca2+ as monitored by fluorescent indicator fura 2. Fura-2 112-118 PCNA clamp associated factor Homo sapiens 0-3 2545786-8 1989 PAF raised the fluorescence of fura 2 at concentrations as low as 8 pM which reached a maximal level about 8 nM, whereas more than nM order concentrations of PAF was required for the detectable release of [3H]AA. Fura-2 31-37 PCNA clamp associated factor Homo sapiens 0-3 2545786-10 1989 However, the fura 2 response to PAF was not effectively inhibited by toxin treatment. Fura-2 13-19 PCNA clamp associated factor Homo sapiens 32-35 2503036-2 1989 Acetylcholine (ACh), tetragastrin (TG), histamine and forskolin induced a transitory increase of intracellular Ca2+ concentration, [Ca2+]i, measured in gastric glands loaded with Ca2+-sensitive dye fura-2, and provoked an acid secretory response evaluated with aminopyrine accumulation ratio (AP ratio). Fura-2 198-204 carbonic anhydrase 2 Oryctolagus cuniculus 111-114 2764121-4 1989 Changes in pHi and Cai2+ were measured with the fluorescent probes, 2,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF) and fura-2. Fura-2 124-130 glucose-6-phosphate isomerase Rattus norvegicus 11-14 2688858-2 1989 The former compounds, especially inositol 1,4,5-triphosphate, are known to cause the release of calcium from intracellular stores, while diacylglycerol is a potent activator of protein kinase C. LHRH causes a rapid and transient increase in intracellular concentrations of free calcium ions, by approximately 4.5-fold, in the majority of granulosa cells as assessed by fura-2 microspectrofluorimetry. Fura-2 369-375 gonadotropin releasing hormone 1 Rattus norvegicus 195-199 2548389-11 1989 The time course of the initial hyperpolarization was shown to be similar to the calcium transient observed in fura-2-loaded A10 cell suspensions after the application of AVP. Fura-2 110-116 arginine vasopressin Rattus norvegicus 170-173 2503036-2 1989 Acetylcholine (ACh), tetragastrin (TG), histamine and forskolin induced a transitory increase of intracellular Ca2+ concentration, [Ca2+]i, measured in gastric glands loaded with Ca2+-sensitive dye fura-2, and provoked an acid secretory response evaluated with aminopyrine accumulation ratio (AP ratio). Fura-2 198-204 carbonic anhydrase 2 Oryctolagus cuniculus 132-135 2663854-5 1989 In fura-2-loaded parotid acinar cells, thapsigargin caused a sustained elevation of [Ca2+], but did not increase inositol phosphate formation. Fura-2 3-9 carbonic anhydrase 2 Homo sapiens 85-88 2503036-2 1989 Acetylcholine (ACh), tetragastrin (TG), histamine and forskolin induced a transitory increase of intracellular Ca2+ concentration, [Ca2+]i, measured in gastric glands loaded with Ca2+-sensitive dye fura-2, and provoked an acid secretory response evaluated with aminopyrine accumulation ratio (AP ratio). Fura-2 198-204 carbonic anhydrase 2 Oryctolagus cuniculus 132-135 2568157-1 1989 Changes of intracellular Ca2+ concentration [( Ca2+]i) in response to somatostatin were measured in cultured rat hippocampal neurons on a single cell basis by fura-2 fluorometry. Fura-2 159-165 somatostatin Rattus norvegicus 70-82 2575164-2 1989 Intracellular free calcium ([Ca2+]i) was monitored by means of Fura-2 fluorescence measurements in hippocampal cells in primary cultures from newborn rats. Fura-2 63-69 carbonic anhydrase 2 Rattus norvegicus 29-32 2499358-8 1989 When the intracellular free Ca2+ concentration ([Ca2+]i) was measured using fura-2, the thrombin-induced [Ca2+]i increase was reduced in platelets which had been treated with a phospholipase A2 inhibitor, ONO-RS-082 (2-(p-amylcinnamoyl)amino-4-chlorobenzoic acid). Fura-2 76-82 coagulation factor II, thrombin Homo sapiens 88-96 2499358-8 1989 When the intracellular free Ca2+ concentration ([Ca2+]i) was measured using fura-2, the thrombin-induced [Ca2+]i increase was reduced in platelets which had been treated with a phospholipase A2 inhibitor, ONO-RS-082 (2-(p-amylcinnamoyl)amino-4-chlorobenzoic acid). Fura-2 76-82 phospholipase A2 group IB Homo sapiens 177-193 2519888-1 1989 Since one of the first measurable events that occurs as a consequence of receptor-mediated cell activation is an increase in the cytosolic free calcium concentration, the calcium-selective fluorescent indicator fura-2 was employed to monitor increases in cytosolic calcium following PAF-acether stimulation of human neutrophils and platelets. Fura-2 211-217 PCNA clamp associated factor Homo sapiens 283-286 2544447-1 1989 In single mouse macrophages stimulated by platelet-activating factor (PAF), the intracellular calcium concentration (Cai) monitored with fura-2 at room temperature presents a biphasic increase, including a transient and a more sustained component. Fura-2 137-143 patchy fur Mus musculus 42-68 2544447-1 1989 In single mouse macrophages stimulated by platelet-activating factor (PAF), the intracellular calcium concentration (Cai) monitored with fura-2 at room temperature presents a biphasic increase, including a transient and a more sustained component. Fura-2 137-143 patchy fur Mus musculus 70-73 2541621-2 1989 Arginine vasopressin (AVP, 100 nM) rapidly increased cytosolic calcium (Caf) measured with fura-2 from a basal level of 65 +/- 5 to 516 +/- 102 nM followed by a return to a plateau level of 128 +/- 18 nM. Fura-2 91-97 vasopressin Sus scrofa 9-20 2540185-10 1989 Fura-2-based microfluorimetry of single dorsal root ganglion neurons demonstrated that both BK and NPY increased cytoplasmic-free Ca2+ concentration and that both peptides could produce this effect in the same neuron. Fura-2 0-6 neuropeptide Y Rattus norvegicus 99-102 2730577-0 1989 Receptor-mediated calcium entry in fura-2-loaded human platelets stimulated with ADP and thrombin. Fura-2 35-41 coagulation factor II, thrombin Homo sapiens 89-97 2730577-2 1989 Previous studies of the early kinetics of rises in cytosolic free [Ca2+] in fura-2-loaded human platelets suggested that: (1) Ca2+ entry slightly preceded internal discharge with thrombin and other agonists known to promote inositol lipid hydrolysis; (2) with ADP, Ca2+ entry occurred without measurable delay and clearly preceded internal Ca2+ discharge. Fura-2 76-82 coagulation factor II, thrombin Homo sapiens 179-187 2524580-3 1989 We report that peptide chemoattractants such as N-formyl-methionyl-leucyl-phenylalanine (fMLP) and the complement fragment C5a in direct comparative studies induced lower amounts of initial Ca2+ mobilization in PMNs from JP patients than healthy controls, as monitored by intracellular fura-2 fluorescence. Fura-2 286-292 formyl peptide receptor 1 Homo sapiens 89-93 2524580-3 1989 We report that peptide chemoattractants such as N-formyl-methionyl-leucyl-phenylalanine (fMLP) and the complement fragment C5a in direct comparative studies induced lower amounts of initial Ca2+ mobilization in PMNs from JP patients than healthy controls, as monitored by intracellular fura-2 fluorescence. Fura-2 286-292 complement C5a receptor 1 Homo sapiens 123-126 2519888-5 1989 The ability of different compounds to antagonize the PAF-acether-induced increase in cytosolic calcium was investigated using quin2 and fura-2 as calcium indicators. Fura-2 136-142 PCNA clamp associated factor Homo sapiens 53-56 2912686-2 1989 In 18 out of 44 individual rat granulosa cells loaded with fura-2, Ang II caused a rapid and transient increase in intracellular free calcium ion concentration, [Ca2+]i. Fura-2 59-65 angiotensinogen Rattus norvegicus 67-73 2460452-6 1988 Accordingly, 1 nM IGF-I caused a gradual increase in cytoplasmic free calcium concentration measured by fura2. Fura-2 104-109 insulin-like growth factor 1 Mus musculus 18-23 2537401-4 1989 In fura-2-loaded, differentiated U-937 cells, PAF induced immediate and concentration-dependent calcium mobilization [( Ca++]i) that was inhibited by CV3988, but not by calcium channel blockers. Fura-2 3-9 PCNA clamp associated factor Homo sapiens 46-49 2537401-4 1989 In fura-2-loaded, differentiated U-937 cells, PAF induced immediate and concentration-dependent calcium mobilization [( Ca++]i) that was inhibited by CV3988, but not by calcium channel blockers. Fura-2 3-9 carbonic anhydrase 1 Homo sapiens 120-126 2909543-1 1989 Bradykinin-evoked rises in [Ca2+]i were measured in fura-2-loaded bovine pulmonary artery endothelial cell monolayers by dual wavelength excitation fluorimetry. Fura-2 52-58 kininogen 1 Bos taurus 0-10 2731227-2 1989 When stimulated with the agonists, angiotensin II and arginine vasopressin, cells incubated with low concentrations of fura-2 or indo-1 (less than 1 microM) produced Ca2+ transients characterized by a small increase followed by a dramatic decrease in fluorescence below the original baseline. Fura-2 119-125 angiotensinogen Rattus norvegicus 35-49 2731227-2 1989 When stimulated with the agonists, angiotensin II and arginine vasopressin, cells incubated with low concentrations of fura-2 or indo-1 (less than 1 microM) produced Ca2+ transients characterized by a small increase followed by a dramatic decrease in fluorescence below the original baseline. Fura-2 119-125 arginine vasopressin Rattus norvegicus 63-74 2473295-4 1989 ET-1 dose-dependently induced increases in [Ca2+]i of both cells loaded with the fluorescent Ca2+ indicator fura-2. Fura-2 108-114 endothelin 1 Rattus norvegicus 0-4 2473325-6 1989 ET-1 and EC-CM induced a dose-dependent increase in steady-state [Ca2+]i in Fura-2-loaded rat VSM cells. Fura-2 76-82 endothelin 1 Rattus norvegicus 0-4 3202164-4 1988 Additionally NPY increased intracellular Ca2+, as assessed by fura-2 fluorescence, with a similar EC50. Fura-2 62-68 neuropeptide Y Homo sapiens 13-16 3177629-6 1988 Dopamine release by PC-12 cells challenged with (R)PAF is associated with a rapid 45Ca influx and efflux and a rise in cytoplasmic calcium concentrations ([Ca2+]i) evaluated by using the calcium indicators fura-2 and quin2. Fura-2 206-212 PCNA clamp associated factor Rattus norvegicus 51-54 3263369-4 1988 Intact fura-2 loaded cells stimulated by thrombin, a physiological agent that causes phosphatidylinositol 4,5-bisphosphate hydrolysis and Ins (1,4,5)P3 release, responded with a rise in cytoplasmic free Ca2+ levels that was dose dependently inhibited by W7(Ki = 25 microM), W13 (Ki = 45 microM), and CGS 9343B (Ki = 110 microM). Fura-2 7-13 coagulation factor II Rattus norvegicus 41-49 3063257-10 1988 In Fura-2-loaded cells GDP[S] inhibited thrombin-induced Ca2+ mobilization, as measured by Fura-2 fluorescence, in a dose-dependent manner. Fura-2 3-9 coagulation factor II, thrombin Homo sapiens 40-48 3063257-10 1988 In Fura-2-loaded cells GDP[S] inhibited thrombin-induced Ca2+ mobilization, as measured by Fura-2 fluorescence, in a dose-dependent manner. Fura-2 91-97 coagulation factor II, thrombin Homo sapiens 40-48 3170551-6 1988 Carbachol (10 microM) or ionomycin (10 microM) stimulation of fura-2-containing cells resulted in a rapid increase in cytosolic free Ca2+ concentration and in the extent of myosin light chain phosphorylation. Fura-2 62-68 myosin heavy chain 14 Homo sapiens 173-179 2846829-8 1988 In fura-2 loaded RBL-1 cells, LTD4 and LTE4 induced a transient intracellular Ca++ mobilization. Fura-2 3-9 RB transcriptional corepressor like 1 Rattus norvegicus 17-22 3263034-5 1988 In addition, bradykinin and melittin stimulated an increase in intracellular calcium concentration in fura-2 loaded endothelial cells and an increase in inositol 1,4,5-trisphosphate (Ins[1,4,5]P3) in cells prelabeled with 3H-myoinositol. Fura-2 102-108 kininogen 1 Bos taurus 13-23 3263034-6 1988 Bradykinin stimulation produced transient increases in Ins(1,4,5)P3, fura-2 fluorescence and transient EDRF release. Fura-2 69-75 kininogen 1 Bos taurus 0-10 3138102-4 1988 [Ca2+]i, measured with the fluorescent dye fura-2, was stimulated by oxytocin and vasopressin (oxytocin greater than vasopressin) in a dose-dependent manner. Fura-2 43-49 arginine vasopressin Homo sapiens 82-93 3196311-4 1988 Here, we have used Mn2+ as the extracellular bivalent cation which can bind to the fluorescent Ca2+ indicator fura-2 to quench its fluorescence completely. Fura-2 110-116 carbonic anhydrase 2 Homo sapiens 95-98 3196311-5 1988 Human umbilical-vein endothelial cells were loaded with fura-2 and, in the presence of extracellular Mn2+, thrombin and histamine were shown to cause quenching of the intracellular dye. Fura-2 56-62 coagulation factor II, thrombin Homo sapiens 107-115 3143354-1 1988 The kinetic features of the changes in the cytosolic free Ca2+ concentration, [Ca2+]i, following stimulation by thyrotropin releasing hormone (TRH) were analysed in single cells of a pituitary line (GH3B6) by dual excitation microfluorimetry [Tsien, Rink & Poenie (1985) Cell Calcium 6, 145-157], using fura 2 as intracellular Ca2+ probe. Fura-2 307-313 thyrotropin releasing hormone Homo sapiens 143-146 3138102-4 1988 [Ca2+]i, measured with the fluorescent dye fura-2, was stimulated by oxytocin and vasopressin (oxytocin greater than vasopressin) in a dose-dependent manner. Fura-2 43-49 arginine vasopressin Homo sapiens 117-128 2851595-2 1988 Fura-2-loaded T51B cells responded with an increase in [Ca2+]i to EGF (5-50 ng/ml) that was blocked by low (25 microM) extracellular Ca2+ or by pretreatment with 50 microM La3+ to inhibit plasma membrane Ca2+ flux. Fura-2 0-6 epidermal growth factor Homo sapiens 66-69 3138987-1 1988 Thapsigargin, a sesquiterpene lactone with potent irritant and tumour-promoting activities, stimulates a rapid (within 15 s) transient increase in intracellular [Ca2+] in the NG115-401L neural cell line, as measured by the fluorescent indicator dye fura-2. Fura-2 249-255 carbonic anhydrase 2 Mus musculus 162-165 3408504-1 1988 The effect of platelet-activating factor (PAF-acether) on cytosolic free calcium, [Ca2+]i, in adherent human vascular endothelial cells in culture was directly determined using a new fluorescent calcium indicator, fura-2. Fura-2 214-220 PCNA clamp associated factor Homo sapiens 42-45 3136667-2 1988 In the present study, measurement of BK-induced changes in fura-2 fluorescence and 86Rb+ efflux were used to monitor changes in cytosolic Ca2+ and K+ permeability in cultured bovine aortic endothelial cells. Fura-2 59-65 kininogen 1 Bos taurus 37-39 3064899-0 1988 [Ca2+]i in single isolated cardiac cells: a review of recent results obtained with digital imaging microscopy and fura-2. Fura-2 114-120 carbonic anhydrase 2 Homo sapiens 1-4 3064899-2 1988 In the studies reviewed in this article, [Ca2+]i in single isolated mammalian ventricular myocytes was observed through the use of the fluorescent Ca2+ indicator, fura-2. Fura-2 163-169 carbonic anhydrase 2 Homo sapiens 42-45 3064899-2 1988 In the studies reviewed in this article, [Ca2+]i in single isolated mammalian ventricular myocytes was observed through the use of the fluorescent Ca2+ indicator, fura-2. Fura-2 163-169 carbonic anhydrase 2 Homo sapiens 147-150 3261750-5 1988 Upon binding to gamma/delta-TCR-bearing Molt-13 cells and PBL, delta TCS1 elicited a fura-2 Caa+ signal indicating that the gamma/delta-receptor is functionally similar to the alpha/beta-heterodimer. Fura-2 85-91 treacle ribosome biogenesis factor 1 Homo sapiens 69-73 3399509-1 1988 The cytosolic calcium (Ca2+i) response to angiotensin II (Ang II) was examined in single rat zona glomerulosa cells by monitoring fura-2 fluorescence with microspectrofluorimetry. Fura-2 130-136 angiotensinogen Rattus norvegicus 42-56 3399509-1 1988 The cytosolic calcium (Ca2+i) response to angiotensin II (Ang II) was examined in single rat zona glomerulosa cells by monitoring fura-2 fluorescence with microspectrofluorimetry. Fura-2 130-136 angiotensinogen Rattus norvegicus 58-64 2450043-0 1988 Parallel measurement of oxoglutarate dehydrogenase activity and matrix free Ca2+ in fura-2-loaded heart mitochondria. Fura-2 84-90 oxoglutarate dehydrogenase Homo sapiens 24-50 3260903-2 1988 Thrombin (0.003-3.0 U/ml) and histamine (10(-7)-10(-4) M) induced rapid increases in [Ca2+]i in suspended cells as monitored with the fluorescent calcium indicator fura-2. Fura-2 164-170 coagulation factor II, thrombin Homo sapiens 0-8 3130276-2 1988 Furthermore, this study, using 45Ca2+, aequorin and fura-2, revealed the mechanism involved in intracellular Ca2+ shifts caused by gastrin and the involvements of inositol 1,4,5-trisphosphate (IP3) and diacylglycerol in producing those shifts. Fura-2 52-58 gastrin Cavia porcellus 131-138 3360779-2 1988 In platelets, thrombin also stimulates Mn2+ entry into the cytosol; this is seen as a stimulated quench of fura-2 fluorescence. Fura-2 107-113 coagulation factor II, thrombin Homo sapiens 14-22 2451660-5 1988 Measurement of intracellular free Ca2+ with fura-2 demonstrated that TGF beta treatment markedly increased the EGF-stimulated rise in free Ca2+ and increased the duration of the response. Fura-2 44-50 transforming growth factor, beta 1 Rattus norvegicus 69-77 3162323-1 1988 The voltage dependence of the intracellular Ca2+ transients was measured in single rat ventricular myocytes with the fluorescent Ca2+ indicator dye fura-2. Fura-2 148-154 carbonic anhydrase 2 Rattus norvegicus 44-47 3162323-1 1988 The voltage dependence of the intracellular Ca2+ transients was measured in single rat ventricular myocytes with the fluorescent Ca2+ indicator dye fura-2. Fura-2 148-154 carbonic anhydrase 2 Rattus norvegicus 129-132 2455515-2 1988 Addition of IFN to the cells loaded with Fura-2, a fluorescent Ca2+ indicator, causes an immediate increase in [Ca2+]i, which is the earliest event after IFN stimulation. Fura-2 41-47 interferon alpha 1 Homo sapiens 12-15 2455515-2 1988 Addition of IFN to the cells loaded with Fura-2, a fluorescent Ca2+ indicator, causes an immediate increase in [Ca2+]i, which is the earliest event after IFN stimulation. Fura-2 41-47 interferon alpha 1 Homo sapiens 154-157 2967092-9 1988 Cholecystokinin induced a rapid increase in cytoplasmic Ca2+ ([Ca2+]i) as estimated with fura-2 and aequorin. Fura-2 89-95 cholecystokinin Homo sapiens 0-15 2967092-9 1988 Cholecystokinin induced a rapid increase in cytoplasmic Ca2+ ([Ca2+]i) as estimated with fura-2 and aequorin. Fura-2 89-95 carbonic anhydrase 2 Homo sapiens 56-59 2967092-9 1988 Cholecystokinin induced a rapid increase in cytoplasmic Ca2+ ([Ca2+]i) as estimated with fura-2 and aequorin. Fura-2 89-95 carbonic anhydrase 2 Homo sapiens 63-66 2967092-14 1988 It appears that the late component of the Ca2+ transient is dependent on Ca2+ influx from the extracellular space and is too localized to be detected by fura-2. Fura-2 153-159 carbonic anhydrase 2 Homo sapiens 42-45 3258153-4 1988 Ten nM EGF increases [Ca2+]i, measured using fura-2, a fluorescent Ca2+ indicator; the EGF-induced [Ca2+]i response occurs immediately, reaches a maximum within several seconds, and then slowly declines. Fura-2 45-51 epidermal growth factor Homo sapiens 7-10 3258153-4 1988 Ten nM EGF increases [Ca2+]i, measured using fura-2, a fluorescent Ca2+ indicator; the EGF-induced [Ca2+]i response occurs immediately, reaches a maximum within several seconds, and then slowly declines. Fura-2 45-51 epidermal growth factor Homo sapiens 87-90 2450043-3 1988 In this paper the correlation between the [Ca2+]m and the in situ activity of oxoglutarate dehydrogenase (OGDH) in fura-2-loaded mitochondria is shown. Fura-2 115-121 oxoglutarate dehydrogenase Homo sapiens 78-104 2450043-3 1988 In this paper the correlation between the [Ca2+]m and the in situ activity of oxoglutarate dehydrogenase (OGDH) in fura-2-loaded mitochondria is shown. Fura-2 115-121 oxoglutarate dehydrogenase Homo sapiens 106-110 2829732-1 1988 In platelets that were loaded with the intracellular calcium indicator fura2, ethanol induced a rapid mobilization of calcium from internal, thrombin-sensitive pools. Fura-2 71-76 coagulation factor II, thrombin Homo sapiens 141-149 2828915-2 1988 In cells loaded with Fura 2, we found that 1 microM oxytocin induced a rapid increase in cytosolic free [Ca2+]i from 120 nM to 250 nM within 12 sec. Fura-2 21-27 oxytocin/neurophysin I prepropeptide Sus scrofa 52-60 2826882-27 1988 In FMLP stimulated neutrophils, the presence of ATP or ADP leads to enhanced increases in intracellular levels of Ca++ as determined by the fura-2 probe, while the presence of AMP or adenosine results in inhibition of the increases in FMLP induced elevations in cytosolic Ca++. Fura-2 140-146 formyl peptide receptor 1 Homo sapiens 3-7 2469884-2 1988 The experiments were carried out on cells loaded with fura-2 using a digital photon-counting camera (PMI VIM) with computer analysis of 16-bit images. Fura-2 54-60 vimentin Rattus norvegicus 105-108 3385691-2 1988 The calcium dependence on the excitation spectrum of fura 2 allows us to choose two wavelengths, lambda 1 and lambda 2, at which an increase in [Ca2+]i causes either a rise or a fall in fluorescence; the ratio of fluorescence at lambda 1 and lambda 2 (R = F lambda 1/F lambda 2) is a measure of [Ca2+]i. Fura-2 53-59 immunoglobulin lambda like polypeptide 2, pseudogene Homo sapiens 256-266 3342003-1 1988 Intracellular free Ca2+ was monitored in suspensions of 1321N1 astrocytoma cells by using the Ca2+ indicator fura-2. Fura-2 109-115 carbonic anhydrase 2 Homo sapiens 19-22 3342003-1 1988 Intracellular free Ca2+ was monitored in suspensions of 1321N1 astrocytoma cells by using the Ca2+ indicator fura-2. Fura-2 109-115 carbonic anhydrase 2 Homo sapiens 94-97 3236338-5 1988 Use of fura2, along with the capacity of Mn2+ ions to effectively quench fura2 fluorescence, provided the basis for a simple and decisive method to determine whether the added CCl4 was permeabilizing the hepatocyte plasma membrane by direct solvent action. Fura-2 7-12 C-C motif chemokine ligand 4 Rattus norvegicus 176-180 3236338-5 1988 Use of fura2, along with the capacity of Mn2+ ions to effectively quench fura2 fluorescence, provided the basis for a simple and decisive method to determine whether the added CCl4 was permeabilizing the hepatocyte plasma membrane by direct solvent action. Fura-2 73-78 C-C motif chemokine ligand 4 Rattus norvegicus 176-180 3347929-6 1988 When effects of cerulenin on intracellular Ca2+ concentration were examined, mobilization of intracellular Ca2+ by thrombin was significantly depressed in the cerulenin-treated platelets as judged by Fura2, Quin2 or chlortetracycline fluorescence. Fura-2 200-205 coagulation factor II, thrombin Homo sapiens 115-123 2451743-8 1987 Thrombin promoted the efflux of 45Ca2+ from cells co-loaded with fura-2 and the isotope. Fura-2 65-71 coagulation factor II, thrombin Homo sapiens 0-8 2962504-2 1987 Resting Ca levels, measured by the use of fura-2, were dose-dependently decreased by up to 30%, by atriopeptin 23. Fura-2 42-48 natriuretic peptide A Homo sapiens 99-110 2443131-1 1987 The ability of bombesin-like peptides to elevate intracellular Ca2+ levels in small cell lung cancer cells was investigated using the fluorescent Ca2+ indicator Fura 2. Fura-2 161-167 gastrin releasing peptide Homo sapiens 15-23 2830242-2 1987 Further findings as to fura2-loaded platelets offered additional support, demonstrating that the initial activation of phospholipase A2 (PLA2) does not require cytoplasmic Ca2+ mobilization. Fura-2 23-28 phospholipase A2 group IB Rattus norvegicus 119-135 2830242-2 1987 Further findings as to fura2-loaded platelets offered additional support, demonstrating that the initial activation of phospholipase A2 (PLA2) does not require cytoplasmic Ca2+ mobilization. Fura-2 23-28 phospholipase A2 group IB Rattus norvegicus 137-141 3323675-7 1987 The intracellular calcium chelator fura 2 also significantly inhibited LIF-mediated degranulation (61.8% inhibition). Fura-2 35-41 LIF interleukin 6 family cytokine Homo sapiens 71-74 2444358-2 1987 After bradykinin (100 nM) exposure, endothelial cell associated fura-2 fluorescence peaked within 10-20 seconds and then declined to a steady level 2- to 3-fold above resting values. Fura-2 64-70 kininogen 1 Bos taurus 6-16 2444358-4 1987 Chelation of extracellular calcium with EGTA before addition of bradykinin resulted in a substantial decrease in the fura-2 transient and elimination of the long-lasting component. Fura-2 117-123 kininogen 1 Bos taurus 64-74 2824759-7 1987 Anagrelide also inhibited the rise in intracellular Ca++ caused by thrombin, as measured using Fura-2-loaded platelets. Fura-2 95-101 coagulation factor II, thrombin Homo sapiens 67-75 2446886-3 1987 Platelet stimulation with thrombin, adenosine diphosphate, platelet activating factor and arachidonic acid resulted in a concentration-dependent 2.5-3-fold increase in cytoplasmic free calcium concentration over the basal levels (140 +/- 32 nM or 104 +/- 21 respectively) as measured with the fluorescent dyes Quin-2 and Fura-2. Fura-2 321-327 coagulation factor II, thrombin Homo sapiens 26-34 3498624-2 1987 Relationships between extracellular calcium ([Ca2+]e), cytosolic free calcium ([Ca2+]i) (as measured with fura-2), and secretion of immunoreactive CGRP and CT have been investigated in rat and human MTC cell lines. Fura-2 106-112 calcitonin-related polypeptide alpha Rattus norvegicus 147-151 3323675-2 1987 Using the fluorescent probe fura 2, we demonstrated that LIF (1/2-2 units) induced a small but significant dose-dependent increase in intracellular calcium in the presence of both calcium-containing (71 nM to 158 nM) and calcium-free (53 nM to 144 nM) buffer solutions. Fura-2 28-34 LIF interleukin 6 family cytokine Homo sapiens 57-60 3653395-1 1987 In the isolated parietal cell the following observations were made: gastrin led to an increase in cytoplasmic free Ca2+ concentration ([Ca2+]i) even in the absence of medium Ca2+ and to transient membrane depolarization in both the absence of the medium Ca2+ and the fura-2-loaded cell. Fura-2 267-273 gastrin Homo sapiens 68-75 3040727-6 1987 Pretreatment with PMA consistently shortened the onset time of the neutrophil"s responses to agonists by about 1 s. When H2O2 production was initiated with PMA, a subsequent stimulation with the agonist fMLP elicited an immediate response (onset time less than 0.2 s) which preceded further changes in fura-2-detected [Ca2+]i. Fura-2 302-308 formyl peptide receptor 1 Homo sapiens 203-207 3032310-6 1987 Thrombin-stimulated rise in platelet cytosolic Ca2+, measured by fura2 fluorescence, and thromboxane A2 formation, measured by radioimmunoassay (RIA), are likewise synergistically inhibited by PGI2 and plasmin. Fura-2 65-70 coagulation factor II, thrombin Homo sapiens 0-8 3672425-2 1987 This was shown by increased aggregatory and secretory responses of quin2 or fura-2 loaded platelets after stimulation with ADP, PAF and with low concentrations of thrombin, collagen, the endoperoxide analog U-46619 and the calcium ionophore A 23187. Fura-2 76-82 coagulation factor II, thrombin Homo sapiens 163-171 2439506-3 1987 By using the Ca2+ indicators quin2 and fura-2, we demonstrate that in PC12 cells voltage-gated Ca2+ channels undergo inactivation dependent on both voltage and [Ca2+]i. Inactivation, however, is never complete and a small number of channels remains open during prolonged depolarization, explaining the steady state elevation of [Ca2+]i observed in cells depolarized with high KCl. Fura-2 39-45 carbonic anhydrase 2 Rattus norvegicus 95-98 2439506-3 1987 By using the Ca2+ indicators quin2 and fura-2, we demonstrate that in PC12 cells voltage-gated Ca2+ channels undergo inactivation dependent on both voltage and [Ca2+]i. Inactivation, however, is never complete and a small number of channels remains open during prolonged depolarization, explaining the steady state elevation of [Ca2+]i observed in cells depolarized with high KCl. Fura-2 39-45 carbonic anhydrase 2 Rattus norvegicus 95-98 2439506-3 1987 By using the Ca2+ indicators quin2 and fura-2, we demonstrate that in PC12 cells voltage-gated Ca2+ channels undergo inactivation dependent on both voltage and [Ca2+]i. Inactivation, however, is never complete and a small number of channels remains open during prolonged depolarization, explaining the steady state elevation of [Ca2+]i observed in cells depolarized with high KCl. Fura-2 39-45 carbonic anhydrase 2 Rattus norvegicus 95-98 2440616-1 1987 [Ca2+]i transients, elicited by voltage-clamp depolarization of single guinea pig cardiac ventricular cells, were observed through use of the fluorescent Ca2+ indicator, fura-2. Fura-2 170-176 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 1-4 2440616-1 1987 [Ca2+]i transients, elicited by voltage-clamp depolarization of single guinea pig cardiac ventricular cells, were observed through use of the fluorescent Ca2+ indicator, fura-2. Fura-2 170-176 LOW QUALITY PROTEIN: carbonic anhydrase 2 Cavia porcellus 154-157 2885709-6 1987 Thus, fura-2 appears to be an adequate probe for resolving second-to-second changes in [Ca++]i induced by hormone receptor activation in anterior pituitary cells. Fura-2 6-12 carbonic anhydrase 1 Rattus norvegicus 88-94 3758190-1 1986 We have investigated the effects of substituting extracellular Na+ by choline or K+ on responses of quin2- and fura-2-loaded human platelets to thrombin and platelet-activating factor (PAF). Fura-2 111-117 coagulation factor II, thrombin Homo sapiens 144-152 3494624-8 1987 These results, as well as additional microscopic fura-2 results in Swiss 3T3 fibroblasts, demonstrate that the Ca2+ signal elicited by EGF is due to two components: redistribution from an intracellular store (possibly mediated by generation of inositol trisphosphate) and stimulated influx across the plasmalemma. Fura-2 49-55 epidermal growth factor Mus musculus 135-138 2884101-2 1987 Changes in intracellular Ca2+, measured using the fluorescent indicator dye Fura-2, show two components; an initial rapid peak in [Ca2+]i which is essentially independent of extracellular Ca2+, and a sustained plateau dependent on the presence of extracellular Ca2+. Fura-2 76-82 carbonic anhydrase 2 Mus musculus 25-28 2884101-2 1987 Changes in intracellular Ca2+, measured using the fluorescent indicator dye Fura-2, show two components; an initial rapid peak in [Ca2+]i which is essentially independent of extracellular Ca2+, and a sustained plateau dependent on the presence of extracellular Ca2+. Fura-2 76-82 carbonic anhydrase 2 Mus musculus 131-134 2884101-2 1987 Changes in intracellular Ca2+, measured using the fluorescent indicator dye Fura-2, show two components; an initial rapid peak in [Ca2+]i which is essentially independent of extracellular Ca2+, and a sustained plateau dependent on the presence of extracellular Ca2+. Fura-2 76-82 carbonic anhydrase 2 Mus musculus 131-134 2884101-2 1987 Changes in intracellular Ca2+, measured using the fluorescent indicator dye Fura-2, show two components; an initial rapid peak in [Ca2+]i which is essentially independent of extracellular Ca2+, and a sustained plateau dependent on the presence of extracellular Ca2+. Fura-2 76-82 carbonic anhydrase 2 Mus musculus 131-134 3112407-2 1987 Using isolated epithelial cells from the toad urinary bladder, Caf has been measured using the intracellular Ca-sensitive fluorescent dyes Fura 2 and Quin 2. Fura-2 139-145 lysine acetyltransferase 2B Homo sapiens 63-66 3755550-1 1986 Continuous measurement and imaging of the intracellular free calcium ion concentration ([Ca2+]i) of mitotic and interphase PtK1 cells was accomplished with the new fluorescent Ca2+ indicator fura-2. Fura-2 191-197 mitogen-activated protein kinase kinase kinase 11 Homo sapiens 123-127 3102480-4 1987 EGF and bradykinin stimulated the rapid accumulation of the two isomers of InsP3, Ins(1,3,4)P3, and Ins(1,4,5)P3 as determined by high performance liquid chromatography analysis; maximal accumulation of Ins(1,4,5)P3 occurred within 15 s. EGF and bradykinin also stimulated a rapid (maximal levels attained within 30 s after addition of hormone) and a sustained 4- and 6-fold rise, respectively, in cytosolic free Ca2+ levels as measured by Fura-2 fluorescence. Fura-2 440-446 epidermal growth factor Homo sapiens 0-3 3102480-4 1987 EGF and bradykinin stimulated the rapid accumulation of the two isomers of InsP3, Ins(1,3,4)P3, and Ins(1,4,5)P3 as determined by high performance liquid chromatography analysis; maximal accumulation of Ins(1,4,5)P3 occurred within 15 s. EGF and bradykinin also stimulated a rapid (maximal levels attained within 30 s after addition of hormone) and a sustained 4- and 6-fold rise, respectively, in cytosolic free Ca2+ levels as measured by Fura-2 fluorescence. Fura-2 440-446 kininogen 1 Homo sapiens 8-18 3792558-0 1987 Stimulation of Ca2+ efflux from fura-2-loaded platelets activated by thrombin or phorbol myristate acetate. Fura-2 32-38 coagulation factor II, thrombin Homo sapiens 69-77 3094510-0 1986 Thrombin and ionomycin can raise platelet cytosolic Ca2+ to micromolar levels by discharge of internal Ca2+ stores: studies using fura-2. Fura-2 130-136 coagulation factor II, thrombin Homo sapiens 0-8 3094510-4 1986 These results with fura-2-loaded platelets indicate that mobilisation of internal Ca2+ can contribute a substantial proportion of the early peak [Ca2+]i evoked by thrombin directly confirming the deductions from previous work with different loadings of quin2. Fura-2 19-25 coagulation factor II, thrombin Homo sapiens 163-171 3758190-1 1986 We have investigated the effects of substituting extracellular Na+ by choline or K+ on responses of quin2- and fura-2-loaded human platelets to thrombin and platelet-activating factor (PAF). Fura-2 111-117 PCNA clamp associated factor Homo sapiens 157-183 3758190-1 1986 We have investigated the effects of substituting extracellular Na+ by choline or K+ on responses of quin2- and fura-2-loaded human platelets to thrombin and platelet-activating factor (PAF). Fura-2 111-117 PCNA clamp associated factor Homo sapiens 185-188 3082623-1 1986 The calcium concentration, [Ca]i, in single rat peritoneal mast cells was measured by means of the new Ca indicator dye fura-2. Fura-2 120-126 carbonic anhydrase 1 Rattus norvegicus 28-32 3014901-6 1986 With fura-2 as the intracellular Ca2+ indicator, [Ca2+]f increased from 74 +/- 7 to a peak of 578 +/- 39 nM (n = 17) with 100 nM AVP. Fura-2 5-11 arginine vasopressin Rattus norvegicus 129-132 3755042-0 1986 Kinetic differences between thrombin-induced and ADP-induced calcium influx and release from internal stores in fura-2-loaded human platelets. Fura-2 112-118 coagulation factor II, thrombin Homo sapiens 28-36 3460085-1 1986 We have used a Ca2+-sensitive dye, fura-2, to investigate the role of Ca2+ during mitosis in Pt K2 epithelial cells. Fura-2 35-41 carbonic anhydrase 2 Homo sapiens 15-18 3004360-2 1986 Cytosolic [Ca2+] was measured using either quin 2 or the recently described indicator fura 2. Fura-2 86-92 carbonic anhydrase 2 Mus musculus 11-14 3840688-4 1985 Platelets loaded with Fura 2 showed a significant rise in cytosolic calcium when stirred with agonists such as epinephrine, arachidonate and thrombin, whereas Quin 2 loaded platelets demonstrated a rise in cytosolic calcium only with thrombin stimulation. Fura-2 22-28 coagulation factor II, thrombin Homo sapiens 141-149