PMID-sentid	Pub_year	Sent_text	compound_name	comp_offset	prot_official_name	organism	prot_offset
33161753-3	2021	Our studies showed that rapid filling of a Mag-Fura-2-sensitive free Ca2+ pool during SERCA1-mediated Ca2+ sequestration appears to be a crucial condition allowing RyR1 Ca2+ channels to close once reloading of luminal Ca2+ stores is complete.	Fura-2	47-53	ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1	Homo sapiens	86-92
32740939-5	2021	The study used the mouse GT1-7 cell line as a model system, and Fura-2 based calcium imaging for detecting the interruption of intracellular calcium transport by the extracellular presence of Gd3+ as released from the CAs; gadodiamide and gadoterate meglumine, when the cells were stimulated in vitro culture by exposure to melatonin.The CA gadoterate meglumine interfered minimally with the calcium signaling, and thus its use is preferable in standard MRI exams.	Fura-2	64-70	GRDX	Homo sapiens	192-195
33161753-3	2021	Our studies showed that rapid filling of a Mag-Fura-2-sensitive free Ca2+ pool during SERCA1-mediated Ca2+ sequestration appears to be a crucial condition allowing RyR1 Ca2+ channels to close once reloading of luminal Ca2+ stores is complete.	Fura-2	47-53	ryanodine receptor 1	Homo sapiens	164-168
33310045-7	2021	Functional TRPM7 in MDA-MB-231, MCF-7, and HEK293 cells were tested with western blotting, patch-clamp, and fura-2 quench assay.	Fura-2	108-114	transient receptor potential cation channel subfamily M member 7	Homo sapiens	11-16
32594194-9	2020	This Ca2+ entry induced by haloperidol was confirmed by Mn2+ entry-induced quench of fura-2 fluorescence.	Fura-2	85-91	carbonic anhydrase 2	Rattus norvegicus	5-8
33255426-9	2020	Fura-2 imaging and extracellular Ca2+ withdrawal confirmed cytosolic Ca2+ transients as a contributing factor of neurotransmitter-induced redox responses and their potentiation in the cytosol of MeCP2-deficient neurons.	Fura-2	0-6	methyl CpG binding protein 2	Mus musculus	195-200
33166100-6	2020	We applied pharmacological as well as CRISPR/Cas9-based strategies to analyse the interplay of TRPM3 and voltage-gated Ca2+ channels in imaging experiments (FMP, Fura-2) and electrophysiological recordings.	Fura-2	162-168	transient receptor potential cation channel, subfamily M, member 3	Rattus norvegicus	95-100
30914247-8	2019	Antimycin A-evoked activation of vagal nociceptors in a Fura2 Ca2+ assay correlated with TRPV1 responses compared to TRPA1 responses.	Fura-2	56-61	transient receptor potential cation channel subfamily V member 1	Homo sapiens	89-94
32047108-6	2020	Using bacterial cells loaded with the fluorescent Fura-2 probe, we further obtained direct biochemical evidence that TMEM165 mediates Ca2+ and Mn2+ influxes.	Fura-2	50-56	transmembrane protein 165	Homo sapiens	117-124
32082084-7	2020	However, fura-2-based fluorescence imaging showed calcium signals upon application of arginine vasopressin or ghrelin within intact pancreatic islets.	Fura-2	9-15	ghrelin	Mus musculus	110-117
31586031-5	2019	The results of a Fura-2 intracellular Ca2+ assay validated Piezo1 as a functional ion channel that is activated by its agonist, Yoda1.	Fura-2	17-23	piezo-type mechanosensitive ion channel component 1	Mus musculus	59-65
31498851-3	2019	METHODS: Using epifluorescence microscopy, we monitored relative changes in the cytosolic fluorescence intensity of Fura2 as a marker of change in intracellular Ca2+ ([Ca2+]i) and relative increases in green fluorescent protein (GFP)-tagged myristoylated alanine-rich C kinase substrate (MARCKS-GFP) as a marker of PKC activation in response to GW9508 at 3 mM and 20 mM glucose.	Fura-2	116-121	myristoylated alanine rich protein kinase C substrate	Rattus norvegicus	241-286
31498851-3	2019	METHODS: Using epifluorescence microscopy, we monitored relative changes in the cytosolic fluorescence intensity of Fura2 as a marker of change in intracellular Ca2+ ([Ca2+]i) and relative increases in green fluorescent protein (GFP)-tagged myristoylated alanine-rich C kinase substrate (MARCKS-GFP) as a marker of PKC activation in response to GW9508 at 3 mM and 20 mM glucose.	Fura-2	116-121	myristoylated alanine rich protein kinase C substrate	Rattus norvegicus	288-294
31498851-3	2019	METHODS: Using epifluorescence microscopy, we monitored relative changes in the cytosolic fluorescence intensity of Fura2 as a marker of change in intracellular Ca2+ ([Ca2+]i) and relative increases in green fluorescent protein (GFP)-tagged myristoylated alanine-rich C kinase substrate (MARCKS-GFP) as a marker of PKC activation in response to GW9508 at 3 mM and 20 mM glucose.	Fura-2	116-121	protein kinase C, alpha	Rattus norvegicus	315-318
31067348-5	2019	Intracellular calcium oscillations of BMMs with RANKL were detected by staining with calcium indicator fura-2 immediately after administration of CTLA4-Ig or after one day of treatment.	Fura-2	103-109	tumor necrosis factor (ligand) superfamily, member 11	Mus musculus	48-53
30508321-6	2019	Based on cobalt mediated quenching effects, calcium imaging experiments using fura-2 showed a concentration-dependent cobalt influx in MG-63 cells, which could be inhibited by the unspecific TRPM7 channel inhibitor 2-APB (20 muM) and TRPM7 specific siRNA.	Fura-2	78-84	transient receptor potential cation channel subfamily M member 7	Homo sapiens	191-196
30508321-6	2019	Based on cobalt mediated quenching effects, calcium imaging experiments using fura-2 showed a concentration-dependent cobalt influx in MG-63 cells, which could be inhibited by the unspecific TRPM7 channel inhibitor 2-APB (20 muM) and TRPM7 specific siRNA.	Fura-2	78-84	transient receptor potential cation channel subfamily M member 7	Homo sapiens	234-239
32540611-4	2020	MATERIALS AND METHODS: In human HNSCC cell lines CAL27 and FaDu and the mouse oral cancer cell line MOC2, P2Y2R contributions to extracellular nucleotide-induced changes in intracellular free Ca2+ concentration and EGFR and extracellular signal-regulated kinase (ERK1/2) phosphorylation were determined using the ratiometric Ca2+ indicator fura-2 and immunoblot analysis, respectively.	Fura-2	340-346	purinergic receptor P2Y2	Homo sapiens	106-111
32466589-4	2020	Therefore, in the present work, the possibility of bioluminescence-driven miniSOG photodynamic CCK1R activation was examined, as monitored by Fura-2 calcium imaging.	Fura-2	142-148	cholecystokinin A receptor	Rattus norvegicus	95-100
32322744-5	2020	Compared to NT, PRL (50 ng/ml) significantly increased the amplitude of Ca2+ transients in myocytes from control (0.084 +- 0.004 vs. 0.115 +- 0.007 Fura-2 ratio units) and diabetic (0.087 +- 0.007 vs. 0.112 +- 0.006 Fura-2 ratio units) heart (n = 36-50 cells).	Fura-2	148-154	prolactin	Rattus norvegicus	16-19
32322744-5	2020	Compared to NT, PRL (50 ng/ml) significantly increased the amplitude of Ca2+ transients in myocytes from control (0.084 +- 0.004 vs. 0.115 +- 0.007 Fura-2 ratio units) and diabetic (0.087 +- 0.007 vs. 0.112 +- 0.006 Fura-2 ratio units) heart (n = 36-50 cells).	Fura-2	216-222	prolactin	Rattus norvegicus	16-19
31805819-11	2020	Ca2+ measurements using the cytosolic indicator Fura-2 and the endoplasmic reticulum luminal store indicator D1ER suggest that TRIC-A enhances Ca2+ leak across the endoplasmic reticulum by directly targeting RyR2 to modulate store overload-induced Ca2+ release.	Fura-2	48-54	transmembrane protein 38A	Homo sapiens	127-133
31834667-6	2020	5-Oxazolecarboxylic acid senses Mg2+ ions, exhibiting a sensitivity of 0.10-0.44 mmol L-1 within the range of 0.5-0.8 mmol L-1 .	Fura-2	0-24	L1 cell adhesion molecule	Homo sapiens	86-89
31834667-6	2020	5-Oxazolecarboxylic acid senses Mg2+ ions, exhibiting a sensitivity of 0.10-0.44 mmol L-1 within the range of 0.5-0.8 mmol L-1 .	Fura-2	0-24	L1 cell adhesion molecule	Homo sapiens	123-126
31444738-2	2019	Fura-2-loaded HEK293-P2X2-PROG cells were acutely plated on top of cultured DH glial cells to quantify ATP release from single DH glial cells.	Fura-2	0-6	purinergic receptor P2X 2	Homo sapiens	21-25
29632074-5	2018	Here, we produced the yeast UPF0016 member Gdt1p in the bacterial host Lactococcus lactis Using Mn2+-induced quenching of Fura-2-emitted fluorescence, we observed that Gdt1p mediates Mn2+ influx, in addition to its previously reported regulation of Ca2+ influx.	Fura-2	122-128	putative ribosome biosynthesis protein GDT1	Saccharomyces cerevisiae S288C	43-48
30139243-8	2018	Mn2+ has been shown to enter cellsthrough similar mechanisms as Ca2+ but quenches fura-2 fluorescence at all excitation wavelengths.Captopril (3000 muM)-induced Mn2+ influx indirectly suggested that captopril evoked Ca2+ entry.Captopril-induced Ca2+ entry was inhibited by 15% by a protein kinase C (PKC) activator (phorbol12-myristate 13 acetate, PMA) and an inhibitor (GF109203X) and three inhibitors of store-operatedCa2+ channels: nifedipine, econazole and SKF96365.	Fura-2	82-88	latexin	Homo sapiens	148-151
29632074-5	2018	Here, we produced the yeast UPF0016 member Gdt1p in the bacterial host Lactococcus lactis Using Mn2+-induced quenching of Fura-2-emitted fluorescence, we observed that Gdt1p mediates Mn2+ influx, in addition to its previously reported regulation of Ca2+ influx.	Fura-2	122-128	putative ribosome biosynthesis protein GDT1	Saccharomyces cerevisiae S288C	168-173
29867546-4	2018	Cold light irradiation (87 mW cm-2) was applied to photosensitizer-expressing cells to examine photodynamic activation of CCK1R by Fura-2 fluorescent calcium imaging.	Fura-2	131-137	cholecystokinin A receptor	Homo sapiens	122-127
28771237-5	2017	[Ca2+]i measurements are most commonly performed in intact single fibers using ratiometric fluorescent indicators such as indo-1 or fura-2.	Fura-2	132-138	carbonic anhydrase 2	Homo sapiens	1-4
29531809-7	2018	Fura-2 measurements on single nuclei and patch-clamp experiments in whole-cell configuration showed that XIP-NLS selectively inhibited nuNCX1.	Fura-2	0-6	late endosomal/lysosomal adaptor, MAPK and MTOR activator 5	Homo sapiens	105-108
28939258-9	2017	Fura-2 imaging was used to also address acute insulin effects on spontaneous and depolarization-mediated Ca2+ transients.	Fura-2	0-6	insulin	Homo sapiens	46-53
28912066-3	2017	cAMP-elevating agents (forskolin and dibutyryl-cAMP) and the joint activation of PKA (6-Bnz-cAMP) and Epac (8-pCPT-2"-O-Me-cAMP) increased cytoplasmic Ca2+ concentration ([Ca2+]c) in <=30% of fura-2-loaded isolated human umbilical vein endothelial cells (HUVEC).	Fura-2	195-201	Rap guanine nucleotide exchange factor 3	Homo sapiens	102-106
28233567-5	2017	Disparity in regulation has been previously reported among the splice variants of NCX3 therefore the activity of Ca2+ uptake and extrusion of the two murine variants was measured using fura-2-based Ca2+ imaging and revealed that both variants are similarly regulated.	Fura-2	185-191	solute carrier family 8 (sodium/calcium exchanger), member 3	Mus musculus	82-86
27443158-6	2017	In whole-cell patch-clamp and intracellular-free calcium ([Ca2+]) concentration (Fura-2) experiments, cumene hydroperoxide and ADP-ribose-induced TRPM2 currents in the neurons were inhibited by N-(p-amylcinnamoyl) anthranilic acid (ACA) and capsaicin-induced TRPV1 currents were inhibited by capsazepine (CPZ) incubations.	Fura-2	81-87	transient receptor potential cation channel, subfamily M, member 2	Rattus norvegicus	146-151
28052871-13	2017	Myoglobin increased the fura 2 fluorescence ratio (cytosolic calcium) during ANG II application (10-12 to 10-6 M).	Fura-2	24-30	myoglobin	Mus musculus	0-9
29043988-3	2017	Concentration of Ca2+ in hippocampus was determined by Fura-2/AM fluorescence probe and the expression of Calbindin-D28K (CB) in hippocampal CA1 was tested by immunohistochemistry.	Fura-2	55-61	carbonic anhydrase 2	Rattus norvegicus	17-20
28052871-13	2017	Myoglobin increased the fura 2 fluorescence ratio (cytosolic calcium) during ANG II application (10-12 to 10-6 M).	Fura-2	24-30	angiotensinogen (serpin peptidase inhibitor, clade A, member 8)	Mus musculus	77-83
26990768-2	2017	METHODS: Fura-2-loaded HEK-293 cells transfected with the histamine H1 receptor was used as a sensitive biosensor assay for histamine release from isolated mouse mesenteric arteries.	Fura-2	9-15	histamine receptor H1	Homo sapiens	58-79
27252039-5	2016	Protriptyline-induced Ca2+ entry was confirmed by Mn2+ -induced quench of fura-2 fluorescence.	Fura-2	74-80	carbonic anhydrase 2	Homo sapiens	22-25
27638877-9	2016	Ca2+ imaging of endothelial cells and rat mesenteric resistance arteries using fura-2, revealed that both GLP-1 analogs caused a reduction in VEGFA-induced Ca2+ signaling.	Fura-2	79-85	glucagon	Rattus norvegicus	106-111
26450366-4	2016	EXPERIMENTAL APPROACH: Thrombin-evoked Ca(2+) fluxes were monitored in Fura-2- or Fluo-5N-loaded human platelets, or using platelet suspensions containing Fluo-4 or Rhod-5N K(+) salts.	Fura-2	71-77	coagulation factor II, thrombin	Homo sapiens	23-31
27371735-6	2016	Measuring intracellular Ca(2+) flux in Fura-2-loaded HASM cultures, we observed that TNF exposure enhanced cytosolic Ca(2+) mobilization and changed the temporal pattern of Ca(2+) flux in individual myocytes induced by NMDA, an NMDA-R selective analog of glutamate.	Fura-2	39-45	tumor necrosis factor	Mus musculus	85-88
26838264-6	2016	Intracellular dialysis of NAADP into Fura-2-loaded DT40TKO-hTPC2 cells elicited cytosolic Ca(2+) transients, suggesting that hTPC2 was functionally active.	Fura-2	37-43	two pore segment channel 2	Homo sapiens	59-64
26838264-6	2016	Intracellular dialysis of NAADP into Fura-2-loaded DT40TKO-hTPC2 cells elicited cytosolic Ca(2+) transients, suggesting that hTPC2 was functionally active.	Fura-2	37-43	two pore segment channel 2	Homo sapiens	125-130
27064319-4	2016	In order to detect the TRPV1-mediated responses in cultured DRG neurons, we monitored intracellular calcium responses to capsaicin, a TRPV1 agonist, with Fura-2.	Fura-2	154-160	transient receptor potential cation channel, subfamily V, member 1	Mus musculus	23-28
25805851-3	2016	Fura-2 loaded cells isolated from young (3-4 months old) and aged (24 months old) mouse responded to acetylcholine (ACh) and cholecystokinin (CCK) with a polarized Ca(2+) response initiated at the secretory pole before spreading to the basal one.	Fura-2	0-6	cholecystokinin	Mus musculus	125-140
25805851-3	2016	Fura-2 loaded cells isolated from young (3-4 months old) and aged (24 months old) mouse responded to acetylcholine (ACh) and cholecystokinin (CCK) with a polarized Ca(2+) response initiated at the secretory pole before spreading to the basal one.	Fura-2	0-6	cholecystokinin	Mus musculus	142-145
27185316-5	2016	The interactions of CSQ1/STIM1 and STIM1/Orai1, and internal Ca2+ changes were detected by co-immunoprecipitation and Fura2, respectively.	Fura-2	118-123	calsequestrin 1	Homo sapiens	20-24
27185316-5	2016	The interactions of CSQ1/STIM1 and STIM1/Orai1, and internal Ca2+ changes were detected by co-immunoprecipitation and Fura2, respectively.	Fura-2	118-123	stromal interaction molecule 1	Homo sapiens	35-40
26434503-9	2015	Thrombin-evoked rises in [Na(+)]cyt were potentiated by PKC inhibition, an effect which was not due to altered changes in non-selective cation permeability of the plasma membrane as assessed by Mn(2+) quench of Fura-2 fluorescence.	Fura-2	211-217	coagulation factor II, thrombin	Homo sapiens	0-8
26427796-5	2015	The degree of platelet-derived growth factor-BB-induced increase in intracellular Ca(2+) was smaller in Fura2-labeled Epac1(-/-)-ASMCs than in Epac1(+/+)-ASMCs.	Fura-2	104-109	Rap guanine nucleotide exchange factor (GEF) 3	Mus musculus	118-123
26047504-5	2015	Functional TRPV4 activity was assessed in cultured RMECs from endothelial Ca2+-responses recorded using fura-2 microfluorimetry and electrophysiological recordings of membrane currents.	Fura-2	104-110	transient receptor potential cation channel, subfamily V, member 4	Rattus norvegicus	11-16
25324545-5	2014	Treatment of C5a with PPAD in vitro resulted in decreased chemotaxis of human neutrophils and diminished calcium signaling in monocytic cell line U937 transfected with the C5a receptor (C5aR) and loaded with a fluorescent intracellular calcium probe: Fura-2 AM.	Fura-2	251-257	complement C5a receptor 1	Homo sapiens	13-16
26053751-4	2015	However, red-shifted versions with green and orange or red FPs (GFP, OFP, RFP) have some advantages such as less phototoxicity and minimal spectral overlay with autofluorescence of cells and fura-2, a prominent chemical Ca2+ indicator.	Fura-2	191-197	tripartite motif containing 27	Homo sapiens	74-77
25767275-11	2015	Imaging of fura-2-loaded SMCs revealed that SMC assemblies displayed coordinated calcium oscillations and cell-cell transmission of calcium waves which, together with increased connexin 43-containing junctions, depended on cadherin-11 and N-cadherin function.	Fura-2	11-17	gap junction protein alpha 1	Homo sapiens	177-188
25767275-11	2015	Imaging of fura-2-loaded SMCs revealed that SMC assemblies displayed coordinated calcium oscillations and cell-cell transmission of calcium waves which, together with increased connexin 43-containing junctions, depended on cadherin-11 and N-cadherin function.	Fura-2	11-17	cadherin 11	Homo sapiens	223-234
25767275-11	2015	Imaging of fura-2-loaded SMCs revealed that SMC assemblies displayed coordinated calcium oscillations and cell-cell transmission of calcium waves which, together with increased connexin 43-containing junctions, depended on cadherin-11 and N-cadherin function.	Fura-2	11-17	cadherin 2	Homo sapiens	239-249
25798335-5	2015	METHODS: As PMCA4 removes cytosolic calcium, we measured transient changes and the time-dependent decay of cytosolic calcium level as visualized by using fura-2 fluorescent dye with confocal microscopy in human SH-SY5Y neuroblastoma cells overexpressing either wild-type or R268Q mutant PMCA4.	Fura-2	154-160	ATPase plasma membrane Ca2+ transporting 4	Homo sapiens	12-17
25395620-7	2015	Furthermore, calcium imaging experiments were performed with fura-2-loaded human embryonic kidney 293 cells overexpressing CysLT1Rs and with isolated mesenteric vascular smooth muscle cells.	Fura-2	61-67	cysteinyl leukotriene receptor 1	Homo sapiens	123-129
24488794-3	2014	The staining method, Fura 2 stain, has sensitivities of 16-32 ng of alpha-casein and beta-casein, 62 ng of ovalbumin, phosvitin, and kappa-casein using an ultraviolet transilluminator.	Fura-2	21-27	casein beta	Homo sapiens	85-96
24471656-5	2014	In enzymatically dissociated human ASM cells loaded with the Ca(2+) indicator, fura-2, AA (1-10 muM) triggered [Ca(2+)]i oscillations that were inhibited by removal of extracellular Ca(2+).	Fura-2	79-85	H19 imprinted maternally expressed transcript	Homo sapiens	35-38
24690333-6	2014	Using video-microscopy to monitor cell contraction, relaxation, and intracellular Ca(2+) transients (Fura-2), we report that incorporation of L48Q cTnC significantly increased contractility of cardiomyocytes from healthy and MI hearts without adversely affecting Ca(2+) transient properties or relaxation.	Fura-2	101-107	troponin C1, slow skeletal and cardiac type	Homo sapiens	147-151
24101294-9	2014	This notion was supported by examining fura-2-loaded isolated AT1A (-/-) and AT1B (-/-) VSMCs.	Fura-2	39-45	angiotensin II receptor, type 1a	Mus musculus	62-66
25239455-3	2014	In retrogradely labelled neurons from the glabrous skin of adult male Sprague-Dawley rats, NCX activity, as assessed with fura-2-based microfluorimetry, was only detected in putative nociceptive IB4+ neurons.	Fura-2	122-128	solute carrier family 8 member A1	Rattus norvegicus	91-94
25080489-7	2014	Both ANG II and the AT1 agonist L162,313 increased the intracellular calcium transients, measured by fura-2, with a two-step pattern.	Fura-2	101-107	angiotensinogen (serpin peptidase inhibitor, clade A, member 8)	Mus musculus	5-11
25080489-7	2014	Both ANG II and the AT1 agonist L162,313 increased the intracellular calcium transients, measured by fura-2, with a two-step pattern.	Fura-2	101-107	angiotensin II receptor, type 1a	Mus musculus	20-23
24951051-4	2014	In the present study, based on the 3D crystal structure of the ZnT-1 homologue, YiiP, that predicts a homodimer that utilizes the H(+) electrochemical gradient to facilitate Zn(2+) efflux, we demonstrate ZnT-1 dependent Zn(2+) efflux from HEK 293T cells using FluoZin-3 and Fura 2 by single cell microscope based fluorescent imaging.	Fura-2	274-280	solute carrier family 30 member 1	Homo sapiens	63-68
24951051-4	2014	In the present study, based on the 3D crystal structure of the ZnT-1 homologue, YiiP, that predicts a homodimer that utilizes the H(+) electrochemical gradient to facilitate Zn(2+) efflux, we demonstrate ZnT-1 dependent Zn(2+) efflux from HEK 293T cells using FluoZin-3 and Fura 2 by single cell microscope based fluorescent imaging.	Fura-2	274-280	solute carrier family 30 member 1	Homo sapiens	204-209
24993595-10	2014	Furthermore, Fura-2 measurement and immuno-blotting analysis, respectively, revealed that PR3 stimulated small intracellular Ca(2+) mobilization and Thr696-specific phosphorylation of the myosin phosphatase target subunit 1 (MYPT1).	Fura-2	13-19	proteinase 3	Homo sapiens	90-93
24993595-10	2014	Furthermore, Fura-2 measurement and immuno-blotting analysis, respectively, revealed that PR3 stimulated small intracellular Ca(2+) mobilization and Thr696-specific phosphorylation of the myosin phosphatase target subunit 1 (MYPT1).	Fura-2	13-19	protein phosphatase 1 regulatory subunit 12A	Homo sapiens	188-223
24828496-9	2014	Fura-2 experiments demonstrated increased activity of TRPV5 in response to dobutamine, which could be prevented by the PKA inhibitor H89.	Fura-2	0-6	transient receptor potential cation channel subfamily V member 5	Homo sapiens	54-59
24488794-3	2014	The staining method, Fura 2 stain, has sensitivities of 16-32 ng of alpha-casein and beta-casein, 62 ng of ovalbumin, phosvitin, and kappa-casein using an ultraviolet transilluminator.	Fura-2	21-27	casein kinase 2 beta	Homo sapiens	118-127
24043696-8	2014	To this end, Orai1 protein abundance was determined by western blotting and SOCE by fura-2 fluorescence.	Fura-2	84-90	ORAI calcium release-activated calcium modulator 1	Homo sapiens	13-18
24685970-12	2014	Use of PTHrP (0.5 mumol/L) also decreased the fluorescence intensity of the indicator for intracellular calcium, Fura-2AM (a membrane-permeable derivative of Fura 2).	Fura-2	158-164	parathyroid hormone-like hormone	Rattus norvegicus	7-12
23831210-5	2013	Here, we show by Fura2 Ca-imaging that pharmacological inhibition of TRPM4 in HL-1 mouse cardiac myocytes by 9-phenanthrol (10 muM) and flufenamic acid (10 and 100 muM) decreases Ca2+ oscillations followed by an overall increase in [Ca2+]i.	Fura-2	17-22	transient receptor potential cation channel, subfamily M, member 4	Mus musculus	69-74
24637628-3	2013	Wild-type or mutant TRPV4 transgenes are commonly expressed in cultured mammalian cells and examined by Fura-2 fluorometry and by electrodes.	Fura-2	104-110	transient receptor potential cation channel subfamily V member 4	Homo sapiens	20-25
24157978-1	2013	We examined the endothelin-1 (ET-1)-induced increase in the intracellular free Ca(2+) concentration ([Ca(2+)]i) in fura-2-loaded rat pulmonary small arteries.	Fura-2	115-121	endothelin 1	Rattus norvegicus	16-28
24157978-1	2013	We examined the endothelin-1 (ET-1)-induced increase in the intracellular free Ca(2+) concentration ([Ca(2+)]i) in fura-2-loaded rat pulmonary small arteries.	Fura-2	115-121	endothelin 1	Rattus norvegicus	30-34
23968883-8	2013	We observed that both cadmium and zinc permeate hTRPV5 in ion imaging experiments using Fura-2 or Newport Green DCF.	Fura-2	88-94	transient receptor potential cation channel subfamily V member 5	Homo sapiens	48-54
23807706-9	2013	Inositol 1,4,5-trisphosphate (IP3) receptors (IP3R) have been functionally demonstrated in submucosal neurons loaded with the Ca(2+)-sensitive fluorescent dye, fura-2.	Fura-2	160-166	inositol 1,4,5-trisphosphate receptor, type 1	Rattus norvegicus	46-50
24036592-7	2014	Functionally, the [Ca(2+)]i response to exogenous angiotensin II was enhanced in fura-2-loaded glomus cells dissociated from hypoxic rats when compared with those of the normoxic control animals.	Fura-2	81-87	angiotensinogen	Rattus norvegicus	50-64
23624270-6	2013	Furthermore, we found that nNOS expression enhances the nicotine-induced increase in the intracellular concentration of Ca(2+), using the Ca(2+)-sensitive fluorescent probe Fura2.	Fura-2	173-178	nitric oxide synthase 1	Rattus norvegicus	27-31
22492652-11	2012	TRPV4-mediated calcium entry was examined in Fura-2-loaded cultured lens epithelium.	Fura-2	45-51	transient receptor potential cation channel subfamily V member 4	Homo sapiens	0-5
23219911-3	2013	Here we examine SERCA function in dissociated sensory neurons using Fura-2 fluorometry.	Fura-2	68-74	ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 3	Homo sapiens	16-21
23345219-4	2013	Using simultaneous fura-2 Ca(2+) imaging and amphotericin B perforated patch-clamp electrophysiology, we find that bradykinin raises [Ca(2+)](i) and induces a biphasic voltage response.	Fura-2	19-25	kininogen 1	Homo sapiens	115-125
23296601-4	2013	Here we describe a single-cell Ca(2+) imaging technique that was used to measure the intracellular Ca(2+) levels to examine the function of Ca(2+)-binding proteins, STIM1 and Calcium release-activated Calcium channel regulator 2A (CRACR2A), using ratiometric Ca(2+) dye Fura-2 in adherent and non-adherent cells.	Fura-2	270-276	calcium release activated channel regulator 2A	Homo sapiens	175-229
23131343-7	2013	The TRPC3 inhibitor, pyrazole-3, prevented ATP- and OAG-dependent transport of Mn(2+) from extracellular stores, demonstrated by a dramatic reduction in the rate of fluorescence quenching of Fura-2.	Fura-2	191-197	transient receptor potential cation channel subfamily C member 3	Homo sapiens	4-9
23797607-4	2013	The Fura-2 Ca2+ imaging revealed that IL-1beta increased intracellular Ca2+ concentration even after removal of extracellular Ca2+, which was blocked by an inhibitor of inositol 1,4,5-trisphosphate receptors, 2-aminoethoxydiphenyl borate (2-APB, 1 muM).	Fura-2	4-10	interleukin 1 beta	Homo sapiens	38-46
23142606-9	2013	WIN reduced TRPV1-induced Ca(2+) transients in fura2-loaded HCEC whereas pertussis toxin (PTX) preincubation obviated suppression by WIN of such rises caused by capsaicin (CAP).	Fura-2	47-52	transient receptor potential cation channel, subfamily V, member 1	Mus musculus	12-17
22897875-5	2012	In Fura-2 Ca(2+) imaging analysis, ghrelin administration increased the intracellular Ca(2+) concentration in approximately 50% of total isolated anterior pituitary cells, and 20% of these cells strongly responded to ghrelin.	Fura-2	3-9	ghrelin and obestatin prepropeptide	Rattus norvegicus	35-42
21993540-4	2012	Bcl-2 overexpressing and control cells were loaded with Fura 2/AM and stimulated with different drugs.	Fura-2	56-62	BCL2, apoptosis regulator	Rattus norvegicus	0-5
22134903-5	2012	P2X7R activation for 2 h did not cause cell death but resulted in a sustained reduction in ER Ca2+ pool size, as evidenced by a diminished cyclopiazonic acid-induced Ca(2+) discharge (fura 2 assay) and a lower fluorescent signal in cells loaded with Mag-fura 2 (ER-specific Ca(2+)-fluorescent dye).	Fura-2	184-190	purinergic receptor P2X, ligand-gated ion channel, 7	Mus musculus	0-5
22134903-5	2012	P2X7R activation for 2 h did not cause cell death but resulted in a sustained reduction in ER Ca2+ pool size, as evidenced by a diminished cyclopiazonic acid-induced Ca(2+) discharge (fura 2 assay) and a lower fluorescent signal in cells loaded with Mag-fura 2 (ER-specific Ca(2+)-fluorescent dye).	Fura-2	254-260	purinergic receptor P2X, ligand-gated ion channel, 7	Mus musculus	0-5
21873674-7	2011	ET-1-induced ONHA fura-2 ratio increases were significantly greater in TSL ONHAs (by 20% at 10-7 M and by 48% at 10-9 M) compared with WT ONHAs.	Fura-2	18-24	endothelin 1	Rattus norvegicus	0-4
21803870-10	2011	In fura 2-loaded ASM cells, [Ca(2+)](i) responses to 1 muM ACh, 10 muM histamine, or 10 nM bradykinin were all exaggerated by TNF-alpha as well as IL-13 exposure.	Fura-2	3-9	kininogen 1	Homo sapiens	91-101
21803870-10	2011	In fura 2-loaded ASM cells, [Ca(2+)](i) responses to 1 muM ACh, 10 muM histamine, or 10 nM bradykinin were all exaggerated by TNF-alpha as well as IL-13 exposure.	Fura-2	3-9	tumor necrosis factor	Homo sapiens	126-135
21803870-10	2011	In fura 2-loaded ASM cells, [Ca(2+)](i) responses to 1 muM ACh, 10 muM histamine, or 10 nM bradykinin were all exaggerated by TNF-alpha as well as IL-13 exposure.	Fura-2	3-9	interleukin 13	Homo sapiens	147-152
23185465-4	2012	RT-PCR and Western blot found that the expression level of Orai1, the pore-forming unit of SOCE, was significantly elevated in the dystrophic muscles, while parallel increases in SOCE activity and SR Ca(2+) storage were detected in adult mdx muscles using Fura-2 fluorescence measurements.	Fura-2	256-262	ORAI calcium release-activated calcium modulator 1	Mus musculus	59-64
22034516-8	2011	Furthermore, calcium imaging with Fura-2 showed that application of L-AP4, an agonist for group III metabotropic glutamate receptors including mGluR6, elevated calcium in endothelial cells.	Fura-2	34-40	glutamate receptor, ionotropic, kainate 2 (beta 2)	Mus musculus	143-149
21873674-8	2011	ET-1-induced fura-2 ratio increases were blocked after pretreatment with BQ-610 (ETA antagonist) in WT and TSL ONHAs, but not by BQ-788 (ETB antagonist) in WT ONHAs.	Fura-2	13-19	endothelin 1	Rattus norvegicus	0-4
21873674-8	2011	ET-1-induced fura-2 ratio increases were blocked after pretreatment with BQ-610 (ETA antagonist) in WT and TSL ONHAs, but not by BQ-788 (ETB antagonist) in WT ONHAs.	Fura-2	13-19	endothelin receptor type A	Rattus norvegicus	81-84
21658429-3	2011	To clarify the pathway underlying AMPK-induced feeding, we monitored the activity of single ARC neurons by measuring cytosolic Ca(2+) concentration ([Ca(2+)](i)) with fura-2 fluorescence imaging.	Fura-2	167-173	protein kinase AMP-activated catalytic subunit alpha 2	Rattus norvegicus	34-38
21810651-4	2011	Thrombin rapidly increased [Ca(2+)](i), as measured by fura-2 fluorescence ratio imaging, in wild-type but not Nox1 null SMCs.	Fura-2	55-61	coagulation factor II	Mus musculus	0-8
21336954-2	2011	Since Ca(2+) plays an important role in cell degeneration, [Ca(2+)]( i ) in alpha-synuclein-overexpressed human glioma cells was analyzed by Fura-2 fluorometry.	Fura-2	141-147	synuclein alpha	Homo sapiens	76-91
21632949-10	2011	Fura2 imaging provided evidence for functional Ca(v)1.3 channels in the LSO of wild-type mice.	Fura-2	0-5	calcium channel, voltage-dependent, L type, alpha 1D subunit	Mus musculus	47-55
21506114-6	2011	In fura2-loaded HCEC, a TRPV1-3 selective agonist, 100 microM 2-aminoethoxydiphenyl borate (2-APB), induced intracellular Ca(2+) transients and an increase in non-selective cation outward currents that were suppressed by ruthenium-red (RuR) (10-20 microM), a non-selective TRPV channel blocker.	Fura-2	3-8	transient receptor potential cation channel subfamily V member 1	Homo sapiens	24-29
21338579-8	2011	Using the fluorescent dye Fura2, we showed in MCF7 that TGZ or Delta2-TGZ induced an immediate increase in cytosolic calcium which was required for ERK1/2 phosphorylation and EGR1 mRNA induction as demonstrated by calcium chelation experiments.	Fura-2	26-31	mitogen-activated protein kinase 3	Homo sapiens	148-154
21338579-8	2011	Using the fluorescent dye Fura2, we showed in MCF7 that TGZ or Delta2-TGZ induced an immediate increase in cytosolic calcium which was required for ERK1/2 phosphorylation and EGR1 mRNA induction as demonstrated by calcium chelation experiments.	Fura-2	26-31	early growth response 1	Homo sapiens	175-179
20678811-3	2010	CCL3 treatment also altered intracellular Ca(2+) dynamics and increased Ca(2+) levels in hippocampal neurons, measured by fura-2 imaging techniques.	Fura-2	122-128	C-C motif chemokine ligand 3	Rattus norvegicus	0-4
21146870-6	2011	Live cell imaging experiments with Fura-2 and NewPort Green DCF showed that overexpression of human TRPV6 increased the permeability for Ca(2+), Ba(2+), Sr(2+), Mn(2+), Zn(2+), Cd(2+), and interestingly also for La(3+) and Gd(3+).	Fura-2	35-41	transient receptor potential cation channel subfamily V member 6	Homo sapiens	100-105
21221778-4	2011	This study shows that treatment of the wild type HEK293 cells with low concentrations of carbachol (1-10 muM), an agonist of the muscarinic receptor, resulted in non-oscillated but sustained [Ca(2+)](i) increase by loading the cells with 1 muM fura2/AM.	Fura-2	244-249	latexin	Homo sapiens	105-108
21221778-5	2011	However, repetitive and long lasting [Ca(2+)](i) oscillations could be induced in 31.1% of the tested cells loaded with 0.1 muM fura2/AM.	Fura-2	128-133	latexin	Homo sapiens	124-127
21541286-8	2011	Endoplasmic reticulum (ER) store depletion by TG enhanced intracellular Ca(2+) levels in loaded with Fura-2 neurons transfected with YFP-STIM1 and ORAI1, but not with YFP-STIM2 and ORAI1, which correlated well with the number of complexes formed.	Fura-2	101-107	stromal interaction molecule 1	Rattus norvegicus	137-142
21858195-8	2011	In fura-2 loaded human ASM cells, NCX-mediated inward Ca(2+) exchange as well as outward exchange (measured as rates of change in [Ca(2+)](i)) was elicited by altering extracellular Na(+) and Ca(2+) levels.	Fura-2	3-9	H19 imprinted maternally expressed transcript	Homo sapiens	23-26
21858195-8	2011	In fura-2 loaded human ASM cells, NCX-mediated inward Ca(2+) exchange as well as outward exchange (measured as rates of change in [Ca(2+)](i)) was elicited by altering extracellular Na(+) and Ca(2+) levels.	Fura-2	3-9	T cell leukemia homeobox 2	Homo sapiens	34-37
21051874-3	2011	MATERIALS AND METHODS: Intracellular calcium concentration ([Ca(2+)](i)) was measured with the indicator dye Fura-2 in response to lysophosphatidic acid, thrombin and sphingosine-1-phosphate.	Fura-2	109-115	coagulation factor II, thrombin	Homo sapiens	154-162
20739625-5	2010	The nifedipine-insensitive transient rise in [Ca(2+)](i) and the increase in Mn(2+) quench of fura-2 fluorescence caused by CPA were both reduced in cells treated with Orai1 siRNA.	Fura-2	94-100	ORAI calcium release-activated calcium modulator 1	Mus musculus	168-173
20592232-2	2010	METHODS: A method for labeling ganglion cell layer (GCL) neurons with the calcium indicator Fura-2 in flat-mounted retinas of adult rats was established.	Fura-2	92-98	germ cell-less 1, spermatogenesis associated	Rattus norvegicus	52-55
20592232-6	2010	The basal intracellular Ca(2+) level, as revealed by the Fura-2 ratio, was elevated in GCL neurons of laser-treated eyes compared with controls.	Fura-2	57-63	germ cell-less 1, spermatogenesis associated	Rattus norvegicus	87-90
20739625-7	2010	Moreover, overexpression of STIM1 enhanced the rise in [Ca(2+)](i) and the increase in Mn(2+) quench of fura-2 fluorescence caused by CPA, and these responses were reduced in cells treated with Orai1 siRNA.	Fura-2	104-110	stromal interaction molecule 1	Mus musculus	28-33
20639579-7	2010	Functional analysis of TRPV1-I585V, using the Ca(2+)-sensitive dye fura-2 to measure intracellular [Ca(2+)] concentrations, revealed a decreased channel activity in response to two typical TRPV1 stimuli, heat and capsaicin.	Fura-2	67-73	transient receptor potential cation channel subfamily V member 1	Homo sapiens	23-28
20802507-10	2010	The ADM-mediated effects were similar to that of forskolin, which activates adenylyl cyclase; additionally, while the PKA inhibitor H89 and Ca2(+) chelator Fura-2 blocked the effect of ADM.	Fura-2	156-162	adrenomedullin	Rattus norvegicus	4-7
20802507-10	2010	The ADM-mediated effects were similar to that of forskolin, which activates adenylyl cyclase; additionally, while the PKA inhibitor H89 and Ca2(+) chelator Fura-2 blocked the effect of ADM.	Fura-2	156-162	adrenomedullin	Rattus norvegicus	185-188
20434434-8	2010	Intracellular Ca(2+) in CA1 region was measured with fura-2 during preconditioning.	Fura-2	53-59	carbonic anhydrase 1	Rattus norvegicus	24-27
20725140-4	2010	Using intact and isolated human hVECs and the Fura-2 calcium (Ca2+) measurement technique, we showed that ET-1 induced a dose-dependent increase of total intracellular free Ca2+, with an EC50 of 1.3 x 10-10 mol/L.	Fura-2	46-52	endothelin 1	Homo sapiens	106-110
20670427-10	2010	Additionally, oxytocin increases cytosolic calcium levels in fura-2-loaded HASMCs that were enhanced in cells treated for 24 hr with IL-13.	Fura-2	61-67	interleukin 13	Homo sapiens	133-138
20381461-6	2010	To test the CaSR functionality, HBEC loaded with fura-2 were stimulated with extracellular Ca(2+) ([Ca(2+)](out)) which resulted in a concentration-dependent intracellular Ca(2+) ([Ca(2+)](i)) increase (potency approximately 5.6mM [Ca(2+)](out)).	Fura-2	49-55	calcium sensing receptor	Homo sapiens	12-16
20714225-7	2010	Calcium ion influx through Ca(V)3.2 was monitored by observing fura-2 fluorescence.	Fura-2	63-69	immunoglobulin lambda variable 7-43	Homo sapiens	27-35
20080989-8	2010	NO-, ANP-, and cGMP-mediated inhibition of the hormone-induced increase in intracellular calcium concentration measured by Fura2 was suppressed in IRAG-deficient VSMC.	Fura-2	123-128	inositol 1,4,5-triphosphate receptor associated 1	Mus musculus	147-151
20413646-6	2010	The authors developed and optimized a fluorescent dye-based bioassay measuring the fluorescence quench of fura-2 by TRPM7-mediated Mn2+ influx in HEK293 cells that stably overexpress TRPM7.	Fura-2	106-112	transient receptor potential cation channel subfamily M member 7	Homo sapiens	116-121
20413646-6	2010	The authors developed and optimized a fluorescent dye-based bioassay measuring the fluorescence quench of fura-2 by TRPM7-mediated Mn2+ influx in HEK293 cells that stably overexpress TRPM7.	Fura-2	106-112	transient receptor potential cation channel subfamily M member 7	Homo sapiens	183-188
20229195-9	2010	Modulation of Ca(2+) signaling by hBD-2 was validated by Fura-2 measurements in human colon carcinoma HT29 cells.	Fura-2	57-63	defensin beta 4A	Homo sapiens	34-39
20237285-8	2010	Using the fura-2 calcium imaging technique, cultured cortical cells responded to GHRH by increasing intracellular calcium.	Fura-2	10-16	growth hormone releasing hormone	Rattus norvegicus	81-85
20307742-5	2010	Ca(2+) influx by reverse NCX activity was measured by fura-2 fluorescence.	Fura-2	54-60	solute carrier family 8 member A1	Rattus norvegicus	25-28
20307742-10	2010	Fura-2 fluorescence measurement revealed that Ca(2+) influx by reverse NCX activity depended on extracellular Ca(2+) concentration, and that this influx was blocked by NCX inhibitor KB-R7943 in a concentration-dependent manner.	Fura-2	0-6	solute carrier family 8 member A1	Rattus norvegicus	71-74
20307742-10	2010	Fura-2 fluorescence measurement revealed that Ca(2+) influx by reverse NCX activity depended on extracellular Ca(2+) concentration, and that this influx was blocked by NCX inhibitor KB-R7943 in a concentration-dependent manner.	Fura-2	0-6	solute carrier family 8 member A1	Rattus norvegicus	168-171
20569277-7	2010	Defining the mechanism(s) by which Grp94 exerts its antioxidant defence, the determination of cytosolic calcium levels in C2C12 cells by fura-2 showed a significantly reduced amount of releasable calcium from intracellular stores, both in conditions of Grp94 overexpression and after curcumin pre-treatment.	Fura-2	137-143	heat shock protein 90, beta (Grp94), member 1	Mus musculus	35-40
20197030-4	2010	Combined patch-clamp and Fura-2 fluorescence recordings revealed that with 2 mM extracellular Ca(2+), and at a membrane potential of -80 mV, approximately 17% of the inward TRPA1 current is carried by Ca(2+).	Fura-2	25-31	transient receptor potential cation channel subfamily A member 1	Homo sapiens	173-178
21063097-10	2010	In K562 cells, G(2)/M cell cycle arrest was preceded by activation of TRPV5/6-like nonselective cation channels in the plasma membrane 1-5 h p.i., resulting in an elevated Ca(2+) entry as evident from fura-2 Ca(2+) imaging.	Fura-2	201-207	transient receptor potential cation channel subfamily V member 5	Homo sapiens	70-77
19913094-6	2010	Measurements with fura-2-loaded cells in suspension revealed that resting [Ca2+]i was elevated and agonist-induced [Ca2+]i increases were augmented in S1P lyase-deficient MEFs both in the presence and absence of extracellular Ca2+.	Fura-2	18-24	sphingosine-1-phosphate receptor 1	Mus musculus	151-154
19996302-5	2009	TRPV6 activity was measured with fluorescence microscopy using Fura-2.	Fura-2	63-69	transient receptor potential cation channel subfamily V member 6	Homo sapiens	0-5
20118617-5	2010	Ca(2+) influx by Na(+)-Ca(2+) exchange, measured by fura-2 fluorescence, showed dependence on extracellular Ca(2+) concentration, and it was blocked by NCX inhibitors KB-R7943, SEA0400, and SN-6.	Fura-2	52-58	solute carrier family 8 member A1	Rattus norvegicus	152-155
19695100-3	2009	RESULTS: Fluorescent imaging of Fura-2 loaded human SW982 synoviocytes reveals immediate and delayed cytosolic calcium oscillations elicited by (1) TRPV1 agonists capsaicin and resiniferatoxin (20-40% of cells), (2) moderate and noxious temperature change, and (3) osmotic stress TRPV4 activation (11.5% of cells).	Fura-2	32-38	transient receptor potential cation channel subfamily V member 1	Homo sapiens	148-153
19695100-3	2009	RESULTS: Fluorescent imaging of Fura-2 loaded human SW982 synoviocytes reveals immediate and delayed cytosolic calcium oscillations elicited by (1) TRPV1 agonists capsaicin and resiniferatoxin (20-40% of cells), (2) moderate and noxious temperature change, and (3) osmotic stress TRPV4 activation (11.5% of cells).	Fura-2	32-38	transient receptor potential cation channel subfamily V member 4	Homo sapiens	280-285
19491242-7	2009	Activation of Epac by 8-pCPT-2"-O-Me-cAMP caused a transient 171.0 +/- 18.0 nM (n = 5) increase in intracellular Ca2+ in Fura-2-loaded aortic myocytes, which persisted in the absence of extracellular Ca2+.	Fura-2	121-127	Rap guanine nucleotide exchange factor 3	Rattus norvegicus	14-18
19386284-5	2009	In fura-2-loaded TRPC1 knockdown cells, despite the decreased TRPC1 levels, cyclopiazonic acid-induced Ca2+ entry and store-operated Ca2+ entry following Ca2+ addition were elevated by 77% and 135%, respectively.	Fura-2	3-9	transient receptor potential cation channel, subfamily C, member 1	Rattus norvegicus	17-22
19386284-5	2009	In fura-2-loaded TRPC1 knockdown cells, despite the decreased TRPC1 levels, cyclopiazonic acid-induced Ca2+ entry and store-operated Ca2+ entry following Ca2+ addition were elevated by 77% and 135%, respectively.	Fura-2	3-9	transient receptor potential cation channel, subfamily C, member 1	Rattus norvegicus	62-67
19523685-6	2009	Signal-regulated Ca(2+) entry (SRCE), defined as a stimulus- and extracellular Ca(2+)-dependent increase in [Ca(2+)](i), was measured in PHM1-41 cells treated with oxytocin (G-protein coupled receptor (GPCR)-stimulated), thapsigargin (store depletion-stimulated), and OAG (diacylglycerol-stimulated), using Fura-2.	Fura-2	307-313	oxytocin/neurophysin I prepropeptide	Homo sapiens	164-172
19307462-4	2009	In cultured human MCs, cell-attached patch clamp and fura-2 fluorescence measurements showed that single knockdown of either TRPC1 or TRPC4 significantly attenuated thapsigargin-induced membrane currents and Ca2+ entry as well as Ang II-induced channel activity.	Fura-2	53-59	transient receptor potential cation channel subfamily C member 1	Homo sapiens	125-130
19487023-3	2009	Using Fura-2-loaded isometric rings of rabbit artery, we found that the Ca(2+)-independent phospholipase A(2) (iPLA(2)) inhibitor, bromoenol lactone (BEL), reduced the KCl-induced tonic but not early phasic phase of T and potentiated [Ca(2+)](i), reducing Ca(2+) sensitization.	Fura-2	6-12	calcium-independent phospholipase A2-gamma	Oryctolagus cuniculus	111-119
19625771-3	2009	Furthermore, ECCE, as measured by Mn(2+) quench of Fura-2, is reconstituted by expression of a mutant DHPR alpha(1S) subunit (SkEIIIK) thought to be impermeable to divalent cations.	Fura-2	51-57	quinoid dihydropteridine reductase	Homo sapiens	102-106
19307462-4	2009	In cultured human MCs, cell-attached patch clamp and fura-2 fluorescence measurements showed that single knockdown of either TRPC1 or TRPC4 significantly attenuated thapsigargin-induced membrane currents and Ca2+ entry as well as Ang II-induced channel activity.	Fura-2	53-59	transient receptor potential cation channel subfamily C member 4	Homo sapiens	134-139
19007332-4	2009	The amount of Ca(2+) released from the ER by a TRPV1 agonist, measured using fura-2, was the same as that released by a SERCA (sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase) inhibitor, indicating that TRPV1 agonist-sensitive stores substantially overlap with SERCA inhibitor-sensitive stores.	Fura-2	77-83	transient receptor potential cation channel subfamily V member 1	Homo sapiens	47-52
19357288-10	2009	Finally, fura-2 calcium imaging indicated that membrane cholesterol alterations might change the vulnerability of cells to Abeta insult by altering calcium influx.	Fura-2	9-15	amyloid beta precursor protein	Homo sapiens	123-128
19157541-6	2009	Furthermore, by patch-clamp and Fura-2-ratiometric measurements we demonstrate that activation of the CaR leads to elevated TRPV5-mediated currents and increases intracellular Ca(2+) concentrations in cells co-expressing TRPV5 and CaR.	Fura-2	32-38	calcium sensing receptor	Homo sapiens	102-105
19157541-6	2009	Furthermore, by patch-clamp and Fura-2-ratiometric measurements we demonstrate that activation of the CaR leads to elevated TRPV5-mediated currents and increases intracellular Ca(2+) concentrations in cells co-expressing TRPV5 and CaR.	Fura-2	32-38	transient receptor potential cation channel subfamily V member 5	Homo sapiens	124-129
19332490-6	2009	The nifedipine-insensitive sustained rise in [Ca(2+)](i) and the increase in Mn(2+) quench of fura-2 fluorescence caused by CPA were both inhibited in cells pretreated with antibody raised against an extracellular epitope of TRPC1.	Fura-2	94-100	transient receptor potential cation channel, subfamily C, member 1	Mus musculus	225-230
19332490-7	2009	Moreover, STIM1 siRNA reduced the rise in [Ca(2+)](i) and Mn(2+) quench of fura-2 fluorescence caused by CPA, whereas overexpression of STIM1 resulted in a marked increase in these responses.	Fura-2	75-81	stromal interaction molecule 1	Mus musculus	10-15
19221709-7	2009	Activation of GPR-54 by kisspeptin also caused reversible increases in [Ca(2+)](i) in Fura-2 loaded dispersed islet cells.	Fura-2	86-92	KISS1 receptor	Mus musculus	14-20
19221709-7	2009	Activation of GPR-54 by kisspeptin also caused reversible increases in [Ca(2+)](i) in Fura-2 loaded dispersed islet cells.	Fura-2	86-92	KiSS-1 metastasis-suppressor	Mus musculus	24-34
19028081-3	2009	Intracellular fura-2 signals in the hippocampal CA3, in which the basal fura-2 signals were higher in zinc deficiency, were significantly more increased 4s after delivery of kainate (1 mM/1 microl, 1 s) to the dentate granule cell layer.	Fura-2	14-20	carbonic anhydrase 3	Rattus norvegicus	48-51
19373968-5	2009	Ratiometric imaging studies with calcium-sensitive dye fura-2 showed that C-INSL7 (10-1000 nM) produced a dose-dependent increase in cytosolic calcium concentrations [Ca2+]i in cultured hypothalamic neurons with two distinct patterns: (1) a sustained elevation lasting for minutes; and (2) a fast, transitory rise followed by oscillations.	Fura-2	55-61	relaxin 3	Rattus norvegicus	76-81
19028081-3	2009	Intracellular fura-2 signals in the hippocampal CA3, in which the basal fura-2 signals were higher in zinc deficiency, were significantly more increased 4s after delivery of kainate (1 mM/1 microl, 1 s) to the dentate granule cell layer.	Fura-2	72-78	carbonic anhydrase 3	Rattus norvegicus	48-51
18204473-4	2008	EXPERIMENTAL APPROACH: Human TRPC5 channels were expressed in HEK 293 cells and studied using fura-2 and patch-clamp recording to measure intracellular calcium and membrane currents respectively at room temperature.	Fura-2	94-100	transient receptor potential cation channel subfamily C member 5	Homo sapiens	29-34
18789885-6	2008	We evaluated the interaction between apoB and cellular membrane by monitoring the change in intracellular Ca(2+) concentration using Fura-2, and found that lipid-free apoB rapidly disrupted the cellular membrane in the absence or presence of the inhibitors for cellular binding/uptake mediated by the receptors.	Fura-2	133-139	apolipoprotein B	Homo sapiens	37-41
18789885-6	2008	We evaluated the interaction between apoB and cellular membrane by monitoring the change in intracellular Ca(2+) concentration using Fura-2, and found that lipid-free apoB rapidly disrupted the cellular membrane in the absence or presence of the inhibitors for cellular binding/uptake mediated by the receptors.	Fura-2	133-139	apolipoprotein B	Homo sapiens	167-171
18478545-5	2008	In parallel, Fura-2 imaging of intracellular Ca(2+) concentration ([Ca(2+)](i)) showed that thrombin induced heterogeneous Ca(2+) responses with asynchronous repetitive peaks.	Fura-2	13-19	coagulation factor II, thrombin	Homo sapiens	92-100
18224450-8	2008	Speculatively, basing our assumptions on Fura2 experiments, we submit the hypothesis that this effect may be due to altered calcium dynamics, which in turn alter cytoskeleton dynamics: the asters, in fact, appear strongly positive to the OTX2 immunoreaction, in both control and exposed samples.	Fura-2	41-46	orthodenticle homeobox 2	Homo sapiens	238-242
18400845-6	2008	Using fura-2 loaded cells, we observed that knocking down the expression of NSP4 totally prevented the increase in Ca(2+) permeability of the plasma membrane and cytosolic Ca(2+) concentration measured in infected cells.	Fura-2	6-12	serine protease 57	Homo sapiens	76-80
18293404-4	2008	These mechanisms were studied in astrocytes in intact optic nerves isolated from transgenic mice expressing enhanced green fluorescent protein (EGFP) under control of the human glial fibrillary acidic protein promoter (GFAP) by Fura-2 ratiometric Ca2+ imaging.	Fura-2	228-234	glial fibrillary acidic protein	Homo sapiens	219-223
18056767-9	2008	In experiments performed in fura-2-loaded myocytes exposed to low Na(+) buffer adjusted to pH 6.4, the lower Ca(2+) uptake indicated an inhibitory effect of acidosis on NCX.	Fura-2	28-34	solute carrier family 8 member A1	Rattus norvegicus	169-172
18289524-4	2008	Measurement of the Ca2+ signal in fura-2-loaded cells allowed to characterize endothelin-1-evoked Ca2+ entry, which was resistant to dihydropyridine, and was blocked by 2-aminoethoxydiphenylborate (2-APB) and micromolar concentration of Gd3+.	Fura-2	34-40	endothelin 1	Homo sapiens	78-90
18568944-5	2008	P2Y(2) receptor desensitization was monitored by following changes in UTP-stimulated intracellular free Ca(2 +) in single cells using fura-2 microfluorometry.	Fura-2	134-140	purinergic receptor P2Y2	Homo sapiens	0-6
17868932-5	2008	By fura-2 microfluorometry, NPW (1-100 nM) concentration-dependently increased cytosolic Ca(2+) concentration ([Ca(2+)](i)) at 8.3 mM glucose in rat single beta-cells.	Fura-2	3-9	neuropeptide W	Rattus norvegicus	28-31
17928534-3	2007	Imaging experiments performed in MIN6 insulinoma cells expressing a genetically encoded cAMP biosensor and loaded with fura-2, a calcium indicator, showed that cAMP oscillations are differentially regulated by periodic changes in membrane potential and GLP-1.	Fura-2	119-125	glucagon	Mus musculus	253-258
17699555-9	2007	Fura-2 fluorescence measurements revealed that the ANG II-induced Ca(2+) influxes were markedly inhibited in MCs with TRPC6 knockdown, reminiscent of the impaired Ca(2+) entry in response to ANG II in high glucose-treated MCs.	Fura-2	0-6	angiotensinogen	Rattus norvegicus	51-57
17675532-2	2007	We found that AGP elicited a minimal rise in [Ca2+]i in Fura-2-loaded neutrophils, and this response was markedly enhanced by pretreatment with anti-L-selectin antibodies.	Fura-2	56-62	selectin L	Homo sapiens	149-159
17959022-4	2007	For the mechanism studies, the intracellular free calcium concentration ([Ca2+]i) and the transcription of caspases-3 and -12 in neurons were detected by Fura 2/AM loading and real-time RT-PCR, respectively.	Fura-2	154-160	caspase 3	Rattus norvegicus	107-125
17687000-8	2007	Treatment of ALA increased the intracellular Ca(2+) concentration measured by fura-2 fluorescent microscopy (P < 0.05), showing that ALA may activate AMPK through enhancing Ca(2+)/calmodulin-dependent protein kinase kinase (CaMKK) signaling.	Fura-2	78-84	protein kinase AMP-activated catalytic subunit alpha 1	Homo sapiens	153-157
17704188-6	2007	In fura 2-loaded ASM cells, [Ca(2+)](i) responses to 1 microM ACh, 10 microM histamine, and 10 nM bradykinin, as well as SOCE, were attenuated (each to a different extent) after disruption of caveolae by the cholesterol-chelating drug methyl-beta-cyclodextrin.	Fura-2	3-9	kininogen 1	Homo sapiens	98-108
17699555-9	2007	Fura-2 fluorescence measurements revealed that the ANG II-induced Ca(2+) influxes were markedly inhibited in MCs with TRPC6 knockdown, reminiscent of the impaired Ca(2+) entry in response to ANG II in high glucose-treated MCs.	Fura-2	0-6	transient receptor potential cation channel, subfamily C, member 6	Rattus norvegicus	118-123
17612974-3	2007	Fura-2 microfluorophotometry indicated that endothelin-1 and endothelin-B receptor selective agonists, endothelin-3 and BQ3020, increased the intracellular calcium concentration of the tissue.	Fura-2	0-6	endothelin-1	Oryctolagus cuniculus	44-56
17670890-10	2007	In the presence of extracellular Ba(2+), OAG-induced elevations of cytosolic Ba(2+) (fura-2 340-to-380-nm ratio) were significantly larger in astrocytes treated with IL-1beta.	Fura-2	85-91	interleukin 1 beta	Mus musculus	166-174
17316820-4	2007	In myoglobin-containing mouse heart endogenous chromophores interfere with Fura-2 fluorometry.	Fura-2	75-81	myoglobin	Mus musculus	3-12
17612974-3	2007	Fura-2 microfluorophotometry indicated that endothelin-1 and endothelin-B receptor selective agonists, endothelin-3 and BQ3020, increased the intracellular calcium concentration of the tissue.	Fura-2	0-6	endothelin receptor type B	Oryctolagus cuniculus	61-82
17612974-3	2007	Fura-2 microfluorophotometry indicated that endothelin-1 and endothelin-B receptor selective agonists, endothelin-3 and BQ3020, increased the intracellular calcium concentration of the tissue.	Fura-2	0-6	endothelin-3	Oryctolagus cuniculus	103-115
17374449-5	2007	In this study, Fura-2 was used to determine [Ca(2+)](i) levels in CA1 hippocampal neurons acutely isolated from young (4-5 months) and mid-age (12-16 months) Sprague-Dawley rats.	Fura-2	15-21	carbonic anhydrase 1	Rattus norvegicus	66-69
17465030-6	2007	The biological activity of SMAP8 in rat central neurons was assessed by the calcium microfluorimetric Fura-2 method.	Fura-2	102-108	NDRG family member 4	Rattus norvegicus	27-32
17125880-6	2007	Also, the temporal pattern of vasopressin-elicited intracellular calcium oscillations studied on fura2-loaded cells was altered, with the duration of each Ca2+ peak being longer.	Fura-2	97-102	arginine vasopressin	Rattus norvegicus	30-41
17264311-6	2007	P2X(7) was barely expressed in HMC and showed a relatively scarce functional activity, as assessed by monitoring nucleotide-induced intracellular calcium surge and plasma membrane depolarization by Fura-2/AM and bis[1,3-diethylthiobarbiturate]trimethineoxonal uptake, respectively.	Fura-2	198-204	purinergic receptor P2X 7	Homo sapiens	0-6
17317768-7	2007	Low extracellular sodium ([Na(+)](o)) solution increases the intracellular Ca(2+) concentration via NCX transporter in fura-2-loaded osteoclasts.	Fura-2	119-125	solute carrier family 8 member A1	Rattus norvegicus	100-103
17389736-11	2007	Fura-2 fluorescence-indicated that Ca(2+) entry in response to AngII stimulation was also dramatically inhibited by TRPC1 antibody and TRPC1-specific RNA interference.	Fura-2	0-6	angiotensinogen	Rattus norvegicus	63-68
17389736-11	2007	Fura-2 fluorescence-indicated that Ca(2+) entry in response to AngII stimulation was also dramatically inhibited by TRPC1 antibody and TRPC1-specific RNA interference.	Fura-2	0-6	transient receptor potential cation channel, subfamily C, member 1	Rattus norvegicus	116-121
17389736-11	2007	Fura-2 fluorescence-indicated that Ca(2+) entry in response to AngII stimulation was also dramatically inhibited by TRPC1 antibody and TRPC1-specific RNA interference.	Fura-2	0-6	transient receptor potential cation channel, subfamily C, member 1	Rattus norvegicus	135-140
17286960-4	2007	By Northern blotting, cDNA cloning, and fura-2 monitoring, the branchial ETR subtype was shown to be ET(A) able to induce a Ca(2+) transit.	Fura-2	40-46	endothelin receptor type A	Homo sapiens	101-106
17446490-3	2007	Here we report that the three isoforms show similar activity evaluated as [Ca2+]i increase evoked by Na+-free medium exposure in Fura-2-loaded single cells and NCX3 transfected cells are less vulnerable to chemical hypoxia compared to NCX1- and NCX2-transfected cells, suggesting that NCX3 could play a more relevant protective role during chemical hypoxia.	Fura-2	129-135	solute carrier family 8 member A1	Homo sapiens	235-239
17446490-3	2007	Here we report that the three isoforms show similar activity evaluated as [Ca2+]i increase evoked by Na+-free medium exposure in Fura-2-loaded single cells and NCX3 transfected cells are less vulnerable to chemical hypoxia compared to NCX1- and NCX2-transfected cells, suggesting that NCX3 could play a more relevant protective role during chemical hypoxia.	Fura-2	129-135	solute carrier family 8 member A2	Homo sapiens	245-249
17446490-3	2007	Here we report that the three isoforms show similar activity evaluated as [Ca2+]i increase evoked by Na+-free medium exposure in Fura-2-loaded single cells and NCX3 transfected cells are less vulnerable to chemical hypoxia compared to NCX1- and NCX2-transfected cells, suggesting that NCX3 could play a more relevant protective role during chemical hypoxia.	Fura-2	129-135	solute carrier family 8 member A3	Homo sapiens	285-289
17209005-8	2007	At the single cardiomyocyte level, VLCAD(-/-) cardiomyocytes showed significant increase in diastolic indo 1 and fura 2 fluorescence, with increased Ca(2+) transient amplitude.	Fura-2	113-119	acyl-Coenzyme A dehydrogenase, very long chain	Mus musculus	35-45
17446466-7	2007	Experiments with Fura-2/AM-loaded cells revealed an increased cytosolic Ca2+ concentration in the cell lines with blocked PMCA2 isoform.	Fura-2	17-23	ATPase plasma membrane Ca2+ transporting 2	Rattus norvegicus	122-127
17982281-4	2007	METHODS: The effect of an immuno-neutralising ECAD on gross morphology, cytosolic calcium signalling, direct cell-to-cell communication and insulin secretion was assessed by fura-2 microfluorimetry, Lucifer Yellow dye injection and insulin ELISA in an insulin-secreting model system.	Fura-2	174-180	cadherin 1	Homo sapiens	46-50
16597917-3	2006	As shown by fura-2 ratio measurements while cells were incubated in a temperature-regulated chamber, significant [Ca(2+)](cyt) elevation was elicited by rapid changes in bath temperature, application of TRPV1 receptor agonists capsaicin and resiniferatoxin, or a cold receptor stimulator, icilin.	Fura-2	12-18	transient receptor potential cation channel subfamily V member 1	Homo sapiens	203-208
16985238-2	2006	Here, we describe an optical technique to simultaneously image cAMP and Ca2+ concentration gradients in MIN6 mouse insulinoma cells using Epac1-camps, a Forster (or fluorescence) resonance energy transfer (FRET)-based cAMP biosensor, and Fura-2, a fluorescent indicator of Ca2+.	Fura-2	238-244	Rap guanine nucleotide exchange factor (GEF) 3	Mus musculus	138-143
16989814-4	2006	Furthermore, Ca(2+) imaging with Fura-2 showed the magnitude of the store-operated Ca(2+) entry to parallel WFS1 expression levels.	Fura-2	33-39	wolframin ER transmembrane glycoprotein	Homo sapiens	108-112
16095882-2	2006	Using fura-2-loaded human platelets we have found that depletion of the TBHQ sensitive store reduces thrombin--but not ADP--or vasopressin (AVP)-induced Ca2+ release.	Fura-2	6-12	coagulation factor II, thrombin	Homo sapiens	101-109
16879472-5	2006	Second, Fura-2 loaded NG-108 cells were incubated with (+)pentazocine, a selective sigma-1 receptor agonist, and propofol and then its fluorescence was observed after stimulation with bradykinin.	Fura-2	8-14	sigma non-opioid intracellular receptor 1	Mus musculus	83-99
16601133-5	2006	INSL5 (100 nm) administered to dissociated and cultured mouse hypothalamic neurons elevated cytosolic calcium concentrations [Ca(2+)](i), as assessed by the microfluorimetric fura-2 method.	Fura-2	175-181	insulin-like 5	Mus musculus	0-5
16600192-5	2006	Fura-2 assay determined significant increases in intracellular free [Ca2+] following exposure to IMN or IFN-gamma.	Fura-2	0-6	interferon gamma	Mus musculus	104-113
16554405-4	2006	Digital imaging microscopy of cells coloaded with the cytosolic Ca2+ indicator fura-2 revealed that, in parallel, rhod-2 maximally decreased the cytosolic Ca2+ removal rate by 20%.	Fura-2	79-85	carbonic anhydrase 2	Homo sapiens	64-67
16554405-4	2006	Digital imaging microscopy of cells coloaded with the cytosolic Ca2+ indicator fura-2 revealed that, in parallel, rhod-2 maximally decreased the cytosolic Ca2+ removal rate by 20%.	Fura-2	79-85	carbonic anhydrase 2	Homo sapiens	155-158
16547971-4	2006	Here, we examined the signal transduction pathway linking activation of chemokine receptor CCR5 to an elevation in [Ca(2+)](i) in cultured microglia by using pharmacological approaches in combination with Fura-2-based digital imaging.	Fura-2	205-211	C-C motif chemokine receptor 5	Homo sapiens	91-95
16250893-8	2006	Finally, cells treated with histamine and loaded with fura 2 exhibited an improved capacity in eliminating high cytosolic Ca2+ concentrations, in accordance with an increase in activity of a low-affinity Ca2+-ATPase, like SERCA 3.	Fura-2	54-60	ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 3	Homo sapiens	222-229
16236332-3	2006	In this study, we studied whether alpha1-adrenoreceptor (alpha1-AR)-activated Ca2+ entry is coupled to both capacitative and non-capacitative pathways in A10 vascular smooth muscle cells by fura-2 fluorescence probe and conventional whole-cell patch clamp techniques.	Fura-2	190-196	immunoglobulin kappa variable 6D-21 (non-functional)	Homo sapiens	154-157
16380530-7	2006	Consistent with the mechanical characteristics, myocytes treated with leptin displayed a reduced electrically stimulated rise in intracellular Ca2+ (change in fura-2 fluorescence intensity) associated with a prolonged intracellular Ca2+ decay rate.	Fura-2	159-165	leptin	Mus musculus	70-76
16247029-3	2005	In parallel measurements with fura-2, CaR activation elicited robust Ca2+ oscillations that increased in frequency in the presence of cAMP, eventually fusing into a sustained plateau.	Fura-2	30-36	calcium sensing receptor	Homo sapiens	38-41
16109841-10	2005	Furthermore, intracellular calcium measurements with fura-2 and fluo-4 showed that activation of the CaSR by Gd(3+) led to a sustained increase in intracellular Ca(2+) even under conditions of low extracellular divalent cations.	Fura-2	53-59	calcium sensing receptor	Homo sapiens	101-105
16410671-5	2005	The mechanism of adrenomedullin action was studied by fura 2-based intracellular calcium measurement.	Fura-2	54-60	adrenomedullin	Homo sapiens	17-31
16409471-11	2006	In FURA 2-loaded platelets, HCy induced a dose-dependent intracellular calcium rise suggesting that the calcium elevation promoted by HCy could participate in the cPLA2 activation, leading to arachidonic acid release and TXB2 formation.	Fura-2	3-9	phospholipase A2 group IVA	Homo sapiens	163-168
15923209-8	2005	TGF-beta (2.5 ng/ml, 18 h) also increased bradykinin-induced intracellular calcium mobilization in fura-2-loaded ASM cells.	Fura-2	99-105	transforming growth factor, beta 1	Mus musculus	0-8
15672411-7	2005	In fura-2 loaded PS1 (a prostate smooth muscle cell line) which express endogenous alpha1A-ARs, alpha-agonists epinephrine (EPI), and phenylephrine (PHE) induced Ca(2+) influx which depended on the extracellular Ca(2+) and PLC activation but was independent of PKC activation.	Fura-2	3-9	presenilin 1	Rattus norvegicus	17-20
15922343-11	2005	Intracellular Ca(2+) concentrations were monitored in the dispersed PRL cells with the Ca(2+)-sensitive dye, fura-2.	Fura-2	109-115	prolactin	Homo sapiens	68-71
16091786-4	2005	Expression of functional PAR2 was determined by RT-PCR and by calcium mobilization in Fura-2/AM-loaded cells.	Fura-2	86-92	F2R like trypsin receptor 1	Homo sapiens	25-29
15802497-6	2005	Using the fluorescent Ca(2+) indicator, fura-2, we determined that ACTH evokes transient increases in [Ca(2+)](i) and elevates basal Ca(2+) levels in resting chondrocytes.	Fura-2	40-46	proopiomelanocortin	Homo sapiens	67-71
15605386-12	2005	According to Fura-2-fluorescence capacitative Ca(2+) entry was lower in sgk1-/- MTF compared to sgk1+/+ MTF.	Fura-2	13-19	serum/glucocorticoid regulated kinase 1	Mus musculus	72-76
16173930-4	2005	The effects of econazole on intracellular Ca2+ concentrations ([Ca2+]i) in and the proliferation of human PC3 prostate cancer cells was explored in the present study using fura-2 and tetrazolium as fluorescent dyes.	Fura-2	172-178	chromobox 8	Homo sapiens	106-109
15855791-4	2005	The FcepsilonRI-induced cellular activation (i.e. cross-linking of FcepsilonRI) was determined by changes in the intracellular level of Ca2+, which was measured by fluorescence of Fura-2.	Fura-2	180-186	Fc epsilon receptor Ia	Homo sapiens	4-15
15921770-5	2005	VIP enhanced intracellular Ca(2+) levels as evaluated using the calcium probe fura-2.	Fura-2	78-84	vasoactive intestinal peptide	Homo sapiens	0-3
15916674-5	2005	The response to TRH was studied using the perforated patch technique and Ca(2+) microfluoromety (fura-2).	Fura-2	97-103	thyrotropin releasing hormone	Rattus norvegicus	16-19
15855791-4	2005	The FcepsilonRI-induced cellular activation (i.e. cross-linking of FcepsilonRI) was determined by changes in the intracellular level of Ca2+, which was measured by fluorescence of Fura-2.	Fura-2	180-186	Fc epsilon receptor Ia	Homo sapiens	67-78
15913603-4	2005	The contribution of the endogenously expressed TRPV1 channel to intracellular calcium concentration increase in the prostate cells was studied by measuring changes in Fura-2 fluorescence by fluorescence microscopy.	Fura-2	167-173	transient receptor potential cation channel subfamily V member 1	Homo sapiens	47-52
15722382-4	2005	METHODS: [Ca(2+)](i) was measured in Fura-2-loaded HEK293(hTRPV1) and HEK293(rTRPV1) cells.	Fura-2	37-43	transient receptor potential cation channel subfamily V member 1	Homo sapiens	58-64
15862567-4	2005	Using Ca(i)2+ imaging and the calcium-sensitive dye fura-2, we found that chicken GnRH-II (cGnRH-II) induced a rapid dose-dependent increase in Ca(i)2+ in dispersed tilapia lactotrophs.	Fura-2	52-58	mitochondrial ribosomal protein S26	Gallus gallus	82-89
15820503-2	2005	Here, using rat TRPV1 heterologously expressed in Chinese hamster ovary cells, we show that different agonists show different patterns of modulation of the intracellular Ca2+ concentration, monitored in individual cells by fura-2 Ca2+ imaging.	Fura-2	223-229	transient receptor potential cation channel, subfamily V, member 1	Rattus norvegicus	16-21
15882549-5	2005	Thrombin-stimulated [Ca2+]i in Ca2+-free and in Ca2+-containing buffers was measured in platelets using the Fura-2 method.	Fura-2	108-114	coagulation factor II	Rattus norvegicus	0-8
15572518-5	2005	The effect of Src kinase inhibition on ANG II-induced intracellular free Ca(2+) concentration ([Ca(2+)](i)) responses was probed in fura 2-loaded preglomerular microvascular smooth muscle cells (PVSMCs) obtained from explants and studied after 3-5 days in culture.	Fura-2	132-138	angiotensinogen	Rattus norvegicus	39-45
15731184-7	2005	Antisense knockdown of the TRPC1 gene, proposed to encode endogenous SOCs, markedly reduces SOCE measured with Fura-2.	Fura-2	111-117	transient receptor potential cation channel, subfamily C, member 1	Mus musculus	27-32
16121035-4	2005	Fura-2 recordings showed that BDNF acutely elicited an increase in intracellular calcium concentration ([Ca2+]i).	Fura-2	0-6	brain derived neurotrophic factor	Homo sapiens	30-34
15634940-2	2005	Because of the known signaling pathways for IL-4 and importance of calcium uptake in maintaining SR calcium stores shared by agonists and caffeine, it was hypothesized that this rapid inhibitory effect might depend on phosphatidylinositol 3-kinase (PI3K) and on inhibition of calcium uptake by the SR. Enzyme-dispersed bovine trachealis cells were loaded with Fura-2/acetoxymethyl ester, and changes in cytosolic calcium were imaged in single cells.	Fura-2	360-366	phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha	Bos taurus	218-247
15716032-5	2005	On the other hand, the basal calcium concentrations in the stratum lucidum and CA3 pyramidal cell layer detected with fura-2 were higher in zinc-deficient mice than in the control mice, indicating that hippocampal calcium homeostasis is affected by zinc deficiency.	Fura-2	118-124	carbonic anhydrase 3	Mus musculus	79-82
15644873-3	2005	In fura-2-loaded neurons, nAChR activation evoked a transient increase in [Ca(2+)](I), which was inhibited reversibly and selectively by clinically relevant concentrations of thiopental.	Fura-2	3-9	cholinergic receptor nicotinic beta 1 subunit	Rattus norvegicus	26-31
15644873-5	2005	In fura-2-loaded neurons, voltage clamped at -60 mV to eliminate any contribution of voltage-gated Ca(2+) channels, thiopental (25 microM) simultaneously inhibited nAChR-induced increases in [Ca(2+)](i) and peak current amplitudes.	Fura-2	3-9	cholinergic receptor nicotinic beta 1 subunit	Rattus norvegicus	164-169
15590764-3	2005	The ability of lymphocyte cholinesterase to inhibit the acetylcholine (ACh)-evoked rise in [Ca(2+)](i) in mouse submandibular acinar cells was determined using fura-2 microfluorimetry.	Fura-2	160-166	butyrylcholinesterase	Homo sapiens	26-40
15731600-4	2005	[Ca2+]i in CA1 neurons was measured with fura-2 and fura-2 FF.	Fura-2	41-47	carbonic anhydrase 2	Rattus norvegicus	1-4
15731600-4	2005	[Ca2+]i in CA1 neurons was measured with fura-2 and fura-2 FF.	Fura-2	41-47	carbonic anhydrase 1	Rattus norvegicus	11-14
15731600-4	2005	[Ca2+]i in CA1 neurons was measured with fura-2 and fura-2 FF.	Fura-2	52-58	carbonic anhydrase 2	Rattus norvegicus	1-4
15731600-4	2005	[Ca2+]i in CA1 neurons was measured with fura-2 and fura-2 FF.	Fura-2	52-58	carbonic anhydrase 1	Rattus norvegicus	11-14
15639476-4	2005	Ang II-induced calcium influx was studied in fura-2-loaded single vascular smooth muscle cells isolated from thoracic aorta of control and diabetic rats.	Fura-2	45-51	angiotensinogen	Rattus norvegicus	0-6
15644863-7	2005	In the stromal compartment of human prostate tissue, immunoreaction with antibodies against the AT(1) receptor was detectable.Fura-2-loaded hPCPs cells showed an instantaneous and linear rise in free intracellular calcium ion concentration ([Ca(2+)](i)) after local perfusion with Ang II in concentrations of 10 nM.	Fura-2	126-132	angiotensinogen	Homo sapiens	281-287
16121035-4	2005	Fura-2 recordings showed that BDNF acutely elicited an increase in intracellular calcium concentration ([Ca2+]i).	Fura-2	0-6	carbonic anhydrase 2	Homo sapiens	105-108
15203106-5	2004	Spectrofluorimetric measurements in Fura-2 loaded cells showed reduced CCE and Mn2+ entry in response to either thapsigargin or 1alpha,25(OH)2D3 upon transfection with anti-TRPC3/6/7 antisense oligodeoxynucleotides (ODNs).	Fura-2	36-42	transient receptor potential cation channel subfamily C member 3	Homo sapiens	173-178
15548706-3	2004	We report evidence from immunofluorescence experiments that in the androgen-responsive LNCaP cell line, the TRPM8 protein is expressed in the endoplasmic reticulum and plasma membrane, acts as a Ca(2+)-permeable channel (assessed using Fura-2 to measure increases in the cytoplasmic Ca(2+) concentration) in each of these membranes, and is regulated by androgen.	Fura-2	236-242	transient receptor potential cation channel subfamily M member 8	Homo sapiens	108-113
15649023-0	2004	Problems caused by high concentration of ATP on activation of the P2X7 receptor in bone marrow cells loaded with the Ca2+ fluorophore fura-2.	Fura-2	134-140	purinergic receptor P2X, ligand-gated ion channel, 7	Mus musculus	66-79
15372487-6	2004	Alterations of the electrophysiological properties of the Cx45(-/-) cardiac myocytes were indicated both by extracellular recording on planar multielectrode array probes and by intracellular Ca(2+) recording of the fluorescent Ca(2+) indicator fura-2.	Fura-2	244-250	gap junction protein, gamma 1	Mus musculus	58-62
15044151-2	2004	Because the canonical transient receptor potential (TRPC) family of proteins was previously shown to comprise Ca(2+)-selective and -nonselective cation channels in a variety of cells, we screened TRPC1-TRPC7 with the use of molecular methods and the fura 2 method to determine their participation as components of the mesangial store-operated Ca(2+) (SOC) channel.	Fura-2	250-256	transient receptor potential cation channel, subfamily C, member 1	Mus musculus	196-201
15326217-4	2004	We measured cytosolic Ca(2+) concentration ([Ca(2+)](i)) in fura-2-loaded VSM of anterior mesenteric artery of Squalus acanthias and show that the ET(B)R agonists IRL 1620 and sarafotoxin S6c (SRX) increase [Ca(2+)](i) in VSM to the same extent as ET-1 and ET(B)R appears to be the only ETR subtype in sharks.	Fura-2	60-66	endothelin receptor type B	Homo sapiens	147-153
15326217-4	2004	We measured cytosolic Ca(2+) concentration ([Ca(2+)](i)) in fura-2-loaded VSM of anterior mesenteric artery of Squalus acanthias and show that the ET(B)R agonists IRL 1620 and sarafotoxin S6c (SRX) increase [Ca(2+)](i) in VSM to the same extent as ET-1 and ET(B)R appears to be the only ETR subtype in sharks.	Fura-2	60-66	endothelin 1	Homo sapiens	248-252
15326217-4	2004	We measured cytosolic Ca(2+) concentration ([Ca(2+)](i)) in fura-2-loaded VSM of anterior mesenteric artery of Squalus acanthias and show that the ET(B)R agonists IRL 1620 and sarafotoxin S6c (SRX) increase [Ca(2+)](i) in VSM to the same extent as ET-1 and ET(B)R appears to be the only ETR subtype in sharks.	Fura-2	60-66	endothelin receptor type B	Homo sapiens	257-263
15362854-5	2004	First, Fura-2 fluorometric Ca(2+) analysis shows the ability of PIP(3) to selectively stimulate [Ca(2+)](i) increase in TRPC6-expressing cells.	Fura-2	7-13	prolactin induced protein	Homo sapiens	64-67
15362854-5	2004	First, Fura-2 fluorometric Ca(2+) analysis shows the ability of PIP(3) to selectively stimulate [Ca(2+)](i) increase in TRPC6-expressing cells.	Fura-2	7-13	transient receptor potential cation channel subfamily C member 6	Homo sapiens	120-125
15309721-8	2004	Fura-2/AM loaded HSC was suspended in no Ca2+ buffer containing 1 mol/L EGTA, 5 min later, 10(-8) mol/L of ET-1 was added, [Ca2+]i inward HSCs rose from resting state to peak 399+/-123 mol/L, then began to come down by the time of 20 min.	Fura-2	0-6	endothelin 1	Rattus norvegicus	107-111
15044151-2	2004	Because the canonical transient receptor potential (TRPC) family of proteins was previously shown to comprise Ca(2+)-selective and -nonselective cation channels in a variety of cells, we screened TRPC1-TRPC7 with the use of molecular methods and the fura 2 method to determine their participation as components of the mesangial store-operated Ca(2+) (SOC) channel.	Fura-2	250-256	transient receptor potential cation channel, subfamily C, member 7	Mus musculus	202-207
15183114-3	2004	Fura-2 fluorometry revealed that the preceding stimulation with trypsin abolished the subsequent [Ca(2+)](i) response to thrombin, while the responses to PAR1-activating peptides remained intact.	Fura-2	0-6	coagulation factor II, thrombin	Homo sapiens	121-129
15245434-5	2004	Application of 100 nM HLE resulted in a transient calcium influx in FURA2-loaded human HaCaT keratinocytes observed by single-cell fluorescence imaging.	Fura-2	68-73	elastase, neutrophil expressed	Homo sapiens	22-25
15481790-5	2004	Intracellular calcium, [Ca2+]i measured by changes in fura-2 level in response to ATP or bradykinin, was significantly inhibited in IgA-IC treated mesangial cells, compared to control cells.	Fura-2	54-60	kininogen 1	Homo sapiens	89-99
14978011-8	2004	Simultaneous measures of contractile tension and intracellular free Ca(2+) (fura 2) levels in arterial rings revealed that significantly more tension was produced per unit change in fura 2 ratio in LCX compared with LAD in response to KCl but not during receptor-agonist stimulation with endothelin-1.	Fura-2	182-188	endothelin-1	Sus scrofa	288-300
15016832-3	2004	Thrombin or thapsigargin added to the external medium activated Ca2+ entry after Ca2+ store depletion, which we monitored by changes in cellular Fura 2 fluorescence.	Fura-2	145-151	coagulation factor II, thrombin	Homo sapiens	0-8
15066999-3	2004	STC-1 cells loaded with fura-2 were briefly (3 min) exposed to saturated FA above and below the threshold length (C(8), C(10), and C(12)).	Fura-2	24-30	stanniocalcin 1	Mus musculus	0-5
15197535-2	2004	Insulin secretion was determined by a radioimmunoassay (RIA), cytosolic free Ca2+ concentration ([Ca2+]c) with the fluorescence dye fura-2 and whole-cell membrane currents with the patch-clamp technique.	Fura-2	132-138	insulin	Homo sapiens	0-7
15048927-3	2004	The expression of CXCR4 receptors was demonstrated further in E17 neurospheres using immunohistochemistry, in situ hybridization, Northern blot analysis and fura-2-based Ca(2+) imaging.	Fura-2	157-163	chemokine (C-X-C motif) receptor 4	Mus musculus	18-23
14966299-3	2004	Peak-systolic intracellular [Ca(2+)] (measured with Fura-2) was higher in the Ad-FKBP12.6 group compared to Ad-LacZ (1 Hz field stimulation at 37 degrees C).	Fura-2	52-58	peptidyl-prolyl cis-trans isomerase FKBP1B	Oryctolagus cuniculus	78-89
14966371-3	2004	Among a variety of Ca(2+)-related agents tested, EGTA and thapsigargin were found to suppress both the glucose uptake and intracellular Ca(2+) mobilization induced by ET-1, as determined by Fura-2 analysis.	Fura-2	190-196	endothelin 1	Homo sapiens	167-171
14724272-10	2004	Fura-2 microfluorimetry revealed that vitronectin induced a significant and sustained increase in intracellular Ca2+ concentration, although vitronectin-induced Ca2+ current could not be detected.	Fura-2	0-6	vitronectin	Bos taurus	38-49
12944324-3	2004	Digital fluorescence microscopy and fura 2 were used to monitor intracellular Ca2+ concentration ([Ca2+]i) changes in response to thrombin and PAR1-activating peptide (PAR1-AP) SFFLRN.	Fura-2	36-42	coagulation factor II, thrombin	Homo sapiens	130-138
12944324-3	2004	Digital fluorescence microscopy and fura 2 were used to monitor intracellular Ca2+ concentration ([Ca2+]i) changes in response to thrombin and PAR1-activating peptide (PAR1-AP) SFFLRN.	Fura-2	36-42	coagulation factor II thrombin receptor	Homo sapiens	143-147
12944324-3	2004	Digital fluorescence microscopy and fura 2 were used to monitor intracellular Ca2+ concentration ([Ca2+]i) changes in response to thrombin and PAR1-activating peptide (PAR1-AP) SFFLRN.	Fura-2	36-42	coagulation factor II thrombin receptor	Homo sapiens	168-172
14733770-8	2004	The cytosolic free calcium concentration induced by UII was measured using Fura-2/AM.	Fura-2	75-81	urotensin 2	Homo sapiens	52-55
12960768-1	2003	A 1-2 min application of brain-derived neurotrophic factor (BDNF; 20 ng/ml) induced sustained elevation of intracellular Ca2+ lasting > 90 min, using the fura-2 imaging of intracellular Ca2+ mobilization, in visual cortical pyramidal neurons isolated from rats.	Fura-2	157-163	brain-derived neurotrophic factor	Rattus norvegicus	25-58
14727511-6	2003	Moreover, fura-2-imaging analyses in midbrain slices demonstrated that nicotine-elicited Ca2+ mobilization was diminished in beta 2-/- mice.	Fura-2	10-16	hemoglobin, beta adult minor chain	Mus musculus	125-131
12829428-6	2003	In this study, the effects of ANG II and KCl on fura 2-measured cytosolic Ca2+ concentration ([Ca2+]i) responses in cultured area postrema neurons in the presence and absence of 12-h exposure to 100 nM 17 beta-estradiol (E2) were evaluated.	Fura-2	48-54	angiotensinogen	Homo sapiens	30-36
14638730-9	2003	Intracellular Ca(2+) ([Ca(2+)](i)) mobilization mediated by ET-1 was measured using the Fura-2 AM fluorescent probe technique as an index of ET receptor function.	Fura-2	88-94	endothelin 1	Homo sapiens	60-64
12908873-9	2003	In the present study, we have used quenched- and stopped-flow approaches to determine the latencies of thrombin-evoked coupling of Ins P3RII with hTRPC1 and of thrombin-evoked bivalent cation entry using Mn2+ quenching of fura 2 fluorescence.	Fura-2	222-228	coagulation factor II, thrombin	Homo sapiens	103-111
12908873-9	2003	In the present study, we have used quenched- and stopped-flow approaches to determine the latencies of thrombin-evoked coupling of Ins P3RII with hTRPC1 and of thrombin-evoked bivalent cation entry using Mn2+ quenching of fura 2 fluorescence.	Fura-2	222-228	coagulation factor II, thrombin	Homo sapiens	160-168
12960768-1	2003	A 1-2 min application of brain-derived neurotrophic factor (BDNF; 20 ng/ml) induced sustained elevation of intracellular Ca2+ lasting > 90 min, using the fura-2 imaging of intracellular Ca2+ mobilization, in visual cortical pyramidal neurons isolated from rats.	Fura-2	157-163	brain-derived neurotrophic factor	Rattus norvegicus	60-64
12595579-5	2003	A deficit in DMPK caused enhanced basal contractility of single cardiomyocytes and an associated increase in intracellular Ca(2+), measured using fura-2.	Fura-2	146-152	dystrophia myotonica-protein kinase	Mus musculus	13-17
12893253-4	2003	The function of the novel murine TRPM4 was studied in HEK-293 cells using a fluorescent calcium indicator, fura-2.	Fura-2	107-113	transient receptor potential cation channel, subfamily M, member 4	Mus musculus	33-38
12805242-5	2003	In voltage-clamped, fura 2-loaded myocytes (20 degrees C), a reduced peak-systolic and end-diastolic [Ca2+]i was observed after Ad-sorcin transfection.	Fura-2	20-26	sorcin	Oryctolagus cuniculus	131-137
12767893-0	2003	Cytoplasmic calcium measurement in rotavirus enterotoxin-enhanced green fluorescent protein (NSP4-EGFP) expressing cells loaded with Fura-2.	Fura-2	133-139	serine protease 57	Homo sapiens	93-97
12815712-3	2003	Using computer-assisted imaging of fura-2 fluorescence, we found an elevation of intracellular cytoplasmic calcium in cortical astrocytes from neonatal Ts65Dn mouse brain, similar to that observed previously in embryonic Ts16 astrocytes.	Fura-2	35-41	reciprocal translocation, Chr 16, cytogenetic band C3-4; and Chr 17, cytogenetic band A2, Davisson 65	Mus musculus	152-158
12494452-2	2003	In this study, the changes in [Ca(2+)](i) elicited by epidermal growth factor (EGF) in rat cardiac microvascular endothelial cells (CMEC) have been investigated by using fura-2 conventional and confocal microscopy.	Fura-2	170-176	epidermal growth factor like 1	Rattus norvegicus	54-77
12614345-7	2003	The mGluR5 receptor function was assessed in CG-4 OPCs with fura-2 microfluorometry.	Fura-2	60-66	glutamate receptor, ionotropic, kainate 1	Mus musculus	4-10
12494452-2	2003	In this study, the changes in [Ca(2+)](i) elicited by epidermal growth factor (EGF) in rat cardiac microvascular endothelial cells (CMEC) have been investigated by using fura-2 conventional and confocal microscopy.	Fura-2	170-176	epidermal growth factor like 1	Rattus norvegicus	79-82
12388389-5	2003	Also, application of ANG II significantly increased intracellular Ca(2+) concentrations, which were measured with fura 2 dye.	Fura-2	114-120	angiotensinogen	Rattus norvegicus	21-27
12479978-5	2003	Angiotensin II evoked an increase in intracellular Ca(2+) in fura-2-loaded human gingival fibroblasts.	Fura-2	61-67	angiotensinogen	Homo sapiens	0-14
12522081-1	2003	1 Using fura-2 fluorometry of [Ca(2+)](i) in response to thrombin, trypsin and protease-activated receptor activating peptides (PAR-APs), we determined whether trypsin cleaves protease-activated receptor 1 (PAR1) and activates it in the endothelial cells of the porcine aortic valves and human umbilical vein.	Fura-2	8-14	coagulation factor II, thrombin	Homo sapiens	57-65
12540526-13	2003	Addition of the bradykinin B(1) receptor agonist [des-Arg(9)]-bradykinin (100 nM) to Fura-2-loaded peritubular cells did not change the [Ca(2+)](i).	Fura-2	85-91	bradykinin receptor B1	Rattus norvegicus	16-40
12522076-1	2003	1 In fura 2-loaded HEK-293 cells stably expressing human type 1 parathyroid hormone (PTH) receptors, PTH potentiated the Ca(2+) mobilization evoked by carbachol by >4 fold without itself increasing the intracellular [Ca(2+)].	Fura-2	5-11	parathyroid hormone	Homo sapiens	64-83
12867160-1	2003	Bradykinin (1 microM) and histamine (100 microM) evoked an initial transient increase and a subsequent sustained increase in intracellular Ca(2+) concentration ([Ca(2+)](i)) in fura-2-loaded human gingival fibroblasts, which may be attributed to Ca(2+) release from intracellular stores and Ca(2+) entry from extracellular sites, respectively.	Fura-2	177-183	kininogen 1	Homo sapiens	0-10
12522076-1	2003	1 In fura 2-loaded HEK-293 cells stably expressing human type 1 parathyroid hormone (PTH) receptors, PTH potentiated the Ca(2+) mobilization evoked by carbachol by >4 fold without itself increasing the intracellular [Ca(2+)].	Fura-2	5-11	parathyroid hormone	Homo sapiens	85-88
12522076-1	2003	1 In fura 2-loaded HEK-293 cells stably expressing human type 1 parathyroid hormone (PTH) receptors, PTH potentiated the Ca(2+) mobilization evoked by carbachol by >4 fold without itself increasing the intracellular [Ca(2+)].	Fura-2	5-11	parathyroid hormone	Homo sapiens	101-104
12508053-8	2003	Fura-2 quenching experiments with Mn(2+), Co(2+), or Ni(2+) suggest that these can be transported by TRPM7 in the presence of physiological levels of Ca(2+) and Mg(2+), suggesting that TRPM7 represents a novel ion-channel mechanism for cellular metal ion entry into vertebrate cells.	Fura-2	0-6	transient receptor potential cation channel subfamily M member 7	Homo sapiens	101-106
12508053-8	2003	Fura-2 quenching experiments with Mn(2+), Co(2+), or Ni(2+) suggest that these can be transported by TRPM7 in the presence of physiological levels of Ca(2+) and Mg(2+), suggesting that TRPM7 represents a novel ion-channel mechanism for cellular metal ion entry into vertebrate cells.	Fura-2	0-6	transient receptor potential cation channel subfamily M member 7	Homo sapiens	185-190
12388458-1	2002	Imaging fluorescent measurements with fura 2 were used to examine cytosolic calcium signals induced by sulfated CCK octapeptide (CCK-8) in dissociated vagal afferent neurons from adult rat nodose ganglia.	Fura-2	38-44	cholecystokinin	Rattus norvegicus	112-115
12509487-1	2003	Functional coupling between Ca(2+)-induced Ca(2+) release (CICR) and quantal exocytosis in 5-hydroxytryptamine-loaded INS-1 beta cells was assessed through the use of carbon fibre amperometry in combination with Fura-2.	Fura-2	212-218	insulin 1	Rattus norvegicus	118-123
12379639-5	2002	The NCKX4 protein demonstrated potassium-dependent sodium calcium exchanger activity when assayed in transfected HEK293 cells using digital imaging of fura-2 fluorescence.	Fura-2	151-157	solute carrier family 24 member 4	Homo sapiens	4-9
12388458-1	2002	Imaging fluorescent measurements with fura 2 were used to examine cytosolic calcium signals induced by sulfated CCK octapeptide (CCK-8) in dissociated vagal afferent neurons from adult rat nodose ganglia.	Fura-2	38-44	cholecystokinin	Rattus norvegicus	129-132
12351388-6	2002	Using Fura-2-loaded platelets, we report that, in line with TRPC6 expression, 1-oleoyl-2-acetyl-sn-glycerol (OAG) stimulated the entry of Ca(++) and Ba(2+) independently of protein kinase C. Thrombin also induced the entry of Ca(++) and Ba(2+), but thapsigargin, which depletes the stores, induced the entry of only Ca(++).	Fura-2	6-12	transient receptor potential cation channel subfamily C member 6	Homo sapiens	60-65
12427859-10	2002	Additionally, exogenous cholecystokinin was effective in elevating intracellular calcium as measured by ratiometric techniques with the calcium-sensitive dye fura-2.	Fura-2	158-164	cholecystokinin	Rattus norvegicus	24-39
12372800-3	2002	Fura 2 fluorescence ratiometry showed that the thapsigargin-induced Ca2+ entry (equivalent to SOC) was significantly inhibited by 1 microM Go-6976 (a specific PKCalpha and betaI inhibitor) and PKCalpha antisense treatment (2.5 nM for 24-48 h).	Fura-2	0-6	protein kinase C alpha	Homo sapiens	159-167
12372800-3	2002	Fura 2 fluorescence ratiometry showed that the thapsigargin-induced Ca2+ entry (equivalent to SOC) was significantly inhibited by 1 microM Go-6976 (a specific PKCalpha and betaI inhibitor) and PKCalpha antisense treatment (2.5 nM for 24-48 h).	Fura-2	0-6	protein kinase C alpha	Homo sapiens	193-201
12411465-8	2002	Moreover, angiotensin II stimulation of divalent cation influx (Mn2+ quench of fura-2 fluorescence) was decreased significantly by AG1478.	Fura-2	79-85	angiotensinogen	Rattus norvegicus	10-24
12405954-9	2002	Fura-2 imaging revealed that a brief application of BDNF elicited a long-lasting elevation of intracellular Ca2+.	Fura-2	0-6	brain-derived neurotrophic factor	Rattus norvegicus	52-56
12217877-4	2002	Using fura 2-loaded preparations, we demonstrated that 10 nM angiotensin II (ANG II) elevates pericyte intracellular Ca(2+) concentration ([Ca(2+)](i)) and suppresses endothelial [Ca(2+)](i).	Fura-2	6-12	angiotensinogen	Homo sapiens	61-75
12217877-4	2002	Using fura 2-loaded preparations, we demonstrated that 10 nM angiotensin II (ANG II) elevates pericyte intracellular Ca(2+) concentration ([Ca(2+)](i)) and suppresses endothelial [Ca(2+)](i).	Fura-2	6-12	angiotensinogen	Homo sapiens	77-83
12383867-1	2002	The responsiveness of cultured myenteric neurons to cholecystokinin (CCK-8) was examined using fura-2-based digital microfluorimetric measurement of intracellular calcium ([Ca(2+)](i)).	Fura-2	95-101	cholecystokinin	Cavia porcellus	69-72
12218532-5	2002	METHODS: The effect of 0.5-3% isoflurane was studied (1) on AngII (10 m)-induced intracellular Ca mobilization, intracellular Ca release from internal stores, and Ca influx in Fura-2 loaded cultured aortic vascular smooth muscle cell isolated from 6-week-old Wistar Kyoto and spontaneously hypertensive rats, using fluorescent imaging microscopy; and (2) on the organization of cytoskeletal elements, using immunofluorescence labeling.	Fura-2	176-182	angiotensinogen	Rattus norvegicus	60-65
12378642-6	2002	Cholecystokinin (CCK)-induced increases in intracellular calcium in single cells were measured with fura-2 microfluorometry.	Fura-2	100-106	cholecystokinin	Rattus norvegicus	0-15
12378642-6	2002	Cholecystokinin (CCK)-induced increases in intracellular calcium in single cells were measured with fura-2 microfluorometry.	Fura-2	100-106	cholecystokinin	Rattus norvegicus	17-20
12213305-3	2002	Ca(2+) stores discharged by application of 100 microM muscarine (in Ca(2+)-free perfusate) in PS1wt and PS1 DeltaE9 cells were significantly larger than those in control cells, as determined using Fura-2 microfluorimetry.	Fura-2	197-203	presenilin 1	Homo sapiens	94-97
12167589-7	2002	We first confirmed that Gd(3+) activated the CaSR by measuring intracellular Ca(2+) concentration ([Ca(2+)](i)) in CALs loaded with fura 2.	Fura-2	132-138	calcium-sensing receptor	Mus musculus	45-49
12167589-7	2002	We first confirmed that Gd(3+) activated the CaSR by measuring intracellular Ca(2+) concentration ([Ca(2+)](i)) in CALs loaded with fura 2.	Fura-2	132-138	carbonic anhydrase 8	Mus musculus	115-119
12210833-4	2002	This investigation, employing fura-2 fluorescent measurements and specific channel blockers and other agents, revealed that GM1 modulates a hitherto unidentified Ca(2+) channel not of the L type.	Fura-2	30-36	coenzyme Q10A	Mus musculus	124-127
12147606-6	2002	ET-1-induced intracellular calcium ([Ca2+]i) in hONAs was measured by fura-2 imaging.	Fura-2	70-76	endothelin 1	Homo sapiens	0-4
12065595-5	2002	Imaging of fura-2-loaded acinar cells revealed that the rate of [Ca(2+)](c) rise during CCK-evoked oscillations slows with each subsequent oscillation, consistent with a developing modulation of release, whereas the kinetics of ACh-evoked oscillations remain constant.	Fura-2	11-17	cholecystokinin	Homo sapiens	88-91
12154174-5	2002	In fura-2-loaded cells extracellular 2-aminoethoxydiphenyl borate and intracellular heparin (5 mg ml(-1)) both inhibited the global cytoplasmic [Ca(2+)] transient evoked by carbachol, confirming that it was IP(3)R-dependent.	Fura-2	3-9	inositol 1,4,5-trisphosphate receptor, type 1	Rattus norvegicus	207-213
12088846-5	2002	Analysis of intracellular calcium dynamics using the calcium-sensitive dye fura-2 AM indicated that exposure of cortical astrocytes to V(1) vasopressin receptor agonist, [Phe(2),Orn(8)]-oxytocin, induced a marked dose-dependent increase in intracellular calcium which was abolished by depletion of extracellular calcium.	Fura-2	75-81	arginine vasopressin	Homo sapiens	140-151
12194015-7	2002	Using fura-2 fluorescence ratiometry to assess [Ca(2+)](i), CCK (<or=10(-10) M)-induced Ca(2+) oscillations were observed in RPAs.	Fura-2	6-12	cholecystokinin	Rattus norvegicus	60-63
11874470-2	2002	Fluorescence ratio imaging indicates that immobilized, aspirin-treated platelets, loaded with Fura-2, respond to inositol 1,4,5-trisphosphate- (InsP3)-generating agonists such as thrombin by high-frequency, irregular rises in cytosolic [Ca2+]i with spikes that vary in peak level and peak-to-peak interval.	Fura-2	94-100	coagulation factor II, thrombin	Homo sapiens	179-187
11926949-12	2002	In fura-2-loaded cells from patients with UC, the NKA-induced Ca(2+) signal was also significantly reduced in Ca(2+)-free medium, indicating the reduced intracellular Ca(2+) stores after UC.	Fura-2	3-9	tachykinin precursor 1	Homo sapiens	50-53
12091553-4	2002	The ability of extracellularly applied U73122 to inhibit PLC was confirmed by its antagonism of ATP-induced elevation of intracellular Ca(2+) as measured by fura-2 photometry.	Fura-2	157-163	heparan sulfate proteoglycan 2	Homo sapiens	57-60
12148843-7	2002	The peak increase of [Ca2+]i induced by cell swelling was 3.40 +/- 0.49 (Fura-2 F340/F380 fluorescence ratio, n = 11) and 3.17 +/- 0.43 (n = 17) in the presence and the absence of extracellular Ca2+, respectively, corresponding to an absolute [Ca2+]i of around 1 microm.	Fura-2	73-79	carbonic anhydrase 2	Homo sapiens	22-25
11781140-1	2002	In fura-2 loaded isolated mouse pancreatic acinar cells, xanthine oxidase (XOD)-catalyzed reactive oxygen species (ROS) generation caused an increase in the cytosolic Ca(2+) concentration ([Ca(2+)](i)) by release of Ca(2+) from intracellular stores.	Fura-2	3-9	xanthine dehydrogenase	Mus musculus	57-73
11889203-6	2002	In fura-2-loaded HEK293 cells expressing the wild-type or mutant receptors, the inactivating R220W mutant produced a significant shift to the right relative to the wild-type CASR in the cytosolic calcium response to increasing extracellular calcium concentrations and the G549R and C850-851 ins/fs mutants were without detectable activity.	Fura-2	3-9	calcium sensing receptor	Homo sapiens	174-178
11750919-10	2002	However, responses to TNF were mimicked by sphingolipids, including sphingosine-1-phosphate, which evoked a transient rises in [Ca2+]i in a pertussis toxin-insensitive manner in fura-2-loaded DRG neurones.	Fura-2	178-184	tumor necrosis factor	Rattus norvegicus	22-25
11818209-8	2002	Accumulation and efflux studies with the P-gp substrates, Dox and Fura-2, demonstrated that Tet inhibited the P-gp-mediated drug efflux.	Fura-2	66-72	ATP binding cassette subfamily B member 1	Homo sapiens	41-45
11818209-8	2002	Accumulation and efflux studies with the P-gp substrates, Dox and Fura-2, demonstrated that Tet inhibited the P-gp-mediated drug efflux.	Fura-2	66-72	ATP binding cassette subfamily B member 1	Homo sapiens	110-114
11773318-8	2002	The I(mtx) activation time course reflected the changes in intracellular Ca(2+) and Na(+) measured with the fluorescent indicators fura-2 and SBFI, respectively, suggesting that the activation of I(mtx) brings about an increment in intracellular Ca(2+) and Na(+).	Fura-2	131-137	metaxin 1	Homo sapiens	6-9
11773318-8	2002	The I(mtx) activation time course reflected the changes in intracellular Ca(2+) and Na(+) measured with the fluorescent indicators fura-2 and SBFI, respectively, suggesting that the activation of I(mtx) brings about an increment in intracellular Ca(2+) and Na(+).	Fura-2	131-137	metaxin 1	Homo sapiens	198-201
11750919-8	2002	TNF-evoked transient increases in [Ca2+]i were also detected in 34% of fura-2-loaded DRG neurones.	Fura-2	71-77	tumor necrosis factor	Rattus norvegicus	0-3
11750919-8	2002	TNF-evoked transient increases in [Ca2+]i were also detected in 34% of fura-2-loaded DRG neurones.	Fura-2	71-77	carbonic anhydrase 2	Rattus norvegicus	35-38
11750919-10	2002	However, responses to TNF were mimicked by sphingolipids, including sphingosine-1-phosphate, which evoked a transient rises in [Ca2+]i in a pertussis toxin-insensitive manner in fura-2-loaded DRG neurones.	Fura-2	178-184	carbonic anhydrase 2	Rattus norvegicus	128-131
11755115-1	2001	In the present study we have employed fura-2 loaded isolated mouse pancreatic acinar cells to monitor the effect that xanthine oxidase (XOD)-catalyzed reactive oxygen species generation presents on Ca(2+) mobilization by the secretagogue cholecystokinin octapeptide (CCK-8).	Fura-2	38-44	xanthine dehydrogenase	Mus musculus	118-134
11755115-1	2001	In the present study we have employed fura-2 loaded isolated mouse pancreatic acinar cells to monitor the effect that xanthine oxidase (XOD)-catalyzed reactive oxygen species generation presents on Ca(2+) mobilization by the secretagogue cholecystokinin octapeptide (CCK-8).	Fura-2	38-44	xanthine dehydrogenase	Mus musculus	136-139
11514552-5	2001	Expression of dFKBP59 produced an inhibition of Ca(2+) influx via TRPL in fura-2 assays.	Fura-2	74-80	FK506-binding protein 59kD	Drosophila melanogaster	14-21
11726808-2	2001	Here we studied ET-1-induced changes in intracellular calcium (Ca2+in) in Fura-2 loaded mouse neuroblastoma-rat dorsal root ganglion hybrid cells (ND7/104).	Fura-2	74-80	endothelin 1	Mus musculus	16-20
11514552-5	2001	Expression of dFKBP59 produced an inhibition of Ca(2+) influx via TRPL in fura-2 assays.	Fura-2	74-80	transient receptor potential-like	Drosophila melanogaster	66-70
11447011-7	2001	Fura 2-loaded SCBN cells had increased fluorescence after PAR-1 activation, indicating increased intracellular Ca(2+).	Fura-2	0-6	coagulation factor II thrombin receptor	Homo sapiens	58-63
11513973-7	2001	Cells expressing hTRPC-1 exhibited a substantial enhancement of maitotoxin-initiated Ca(2+) inflow and a modest enhancement of thapsigargin-initiated Ca(2+) inflow (measured using fura-2) and no enhancement of the highly Ca(2+)-selective store-operated Ca(2+) current (measured using patch-clamp recording).	Fura-2	180-186	transient receptor potential cation channel subfamily C member 1	Homo sapiens	17-24
11487506-5	2001	Thrombin and the PAR1 agonist TFLLR-NH(2) (TF-NH(2)) increased [Ca(2+)](i) >50% of cultured neurons (EC(50)s 24 mu ml(-1) and 1.9 microM, respectively), assessed using Fura-2 AM.	Fura-2	171-177	coagulation factor II	Rattus norvegicus	0-8
11487506-5	2001	Thrombin and the PAR1 agonist TFLLR-NH(2) (TF-NH(2)) increased [Ca(2+)](i) >50% of cultured neurons (EC(50)s 24 mu ml(-1) and 1.9 microM, respectively), assessed using Fura-2 AM.	Fura-2	171-177	coagulation factor II (thrombin) receptor	Rattus norvegicus	17-21
11448475-3	2001	In this study, we have measured capsaicin- and anandamide-induced elevations in intracellular calcium concentrations ([Ca(2+)](i)) in fura-2 loaded HEK293 cells stably expressing the rat VR1 receptor at 22, 37 and 50 degrees C. Both capsaicin and anandamide produced a concentration-dependent elevation in [Ca(2+)](i) at all temperatures.	Fura-2	134-140	transient receptor potential cation channel, subfamily V, member 1	Rattus norvegicus	187-190
11483701-2	2001	To determine the mechanism of bradykinin-induced production of endothelium-derived contracting factors, we monitored the changes in cytosolic Ca(2+) concentration ([Ca(2+)](i)) in in situ endothelial cells in porcine aortic valvular strips and the changes in [Ca(2+)](i) of smooth muscle cells and force in porcine interlobar renal arterial strips using front-surface fluorometry of fura-2.	Fura-2	383-389	kininogen 1	Homo sapiens	30-40
11171105-2	2001	In freshly-isolated rat hepatocytes, 2-APB inhibited thapsigargin- and vasopressin-stimulated Ca2+ inflow (measured using fura-2) with no detectable effect on the release of Ca2+ from intracellular stores.	Fura-2	122-128	arginyl aminopeptidase	Rattus norvegicus	39-42
11294880-4	2001	As anticipated from this sequence similarity, NCKX3 displayed K(+)-dependent Na(+)/Ca(2+) exchanger activity when assayed in heterologous expression systems, using digital imaging of fura-2 fluorescence, electrophysiology, or radioactive (45)Ca(2+) uptake.	Fura-2	183-189	solute carrier family 24 member 3	Homo sapiens	46-51
11373474-6	2001	The effect of anesthetics on NMDA receptors was determined by measuring the NMDA-mediated changes in intracellular calcium in the CA1 pyramidal layer with a calcium indicator, fura-2.	Fura-2	176-182	carbonic anhydrase 1	Rattus norvegicus	130-133
11287471-8	2001	Cellular Ca(2+) fluorescence measurement using fura-2 indicated that neurotensin rapidly induced Ca(2+) release from intracellular stores of PAG-RVM neurons.	Fura-2	47-53	neurotensin	Rattus norvegicus	69-80
11171105-9	2001	In the H4-IIE rat hepatoma cell line, 2-APB inhibited thapsigargin-stimulated Ca2+ inflow (measured using fura-2) and, in whole-cell patch-clamp experiments, the Ins(1,4,5)P(3)-induced inward current carried by Ca2+.	Fura-2	106-112	arginyl aminopeptidase	Rattus norvegicus	40-43
11240034-6	2001	Direct application of either human IgM anti-GM1 antibodies or the B subunit of CT to N18 neuroblastoma cells induced a sustained influx of manganese ions, as indicated by a quench of the intracellular fura-2 fluorescence.	Fura-2	201-207	coenzyme Q10A	Mus musculus	44-47
11250874-2	2001	This study investigates the mechanism of CGRP-induced relaxation in intramural coronary arteries by determining the effect of CGRP on cytosolic Ca(2+) concentration ([Ca(2+)](i)) using FURA-2 technique.	Fura-2	185-191	calcitonin-related polypeptide alpha	Rattus norvegicus	41-45
11281550-3	2001	METHODS AND RESULTS: Stimulation of HSCs with ADM resulted in a dose-dependent raise in cAMP concentration (radioimmunoassay) and markedly blunted the endothelin-induced increase in [Ca2+]i and cell contraction, as assessed in cells loaded with fura-2 using a morphometric method.	Fura-2	245-251	adrenomedullin	Homo sapiens	46-49
11805429-1	2001	BACKGROUND: The effect of bradykinin on intracellular free Ca(2+) levels ([Ca(2+)](i)) in MG63 human osteosarcoma cells was explored using fura-2 as a Ca(2+) dye.	Fura-2	139-145	kininogen 1	Homo sapiens	26-36
11509826-5	2001	Ca(i) of primary cultured rat alveolar type II cells was measured by fura-2 epifluorescence.	Fura-2	69-75	carbonic anhydrase 1	Rattus norvegicus	0-5
11136854-1	2001	Patch clamp and fura-2 fluorescence were employed to characterize receptor-mediated activation of recombinant Drosophila TrpL channels expressed in Sf9 insect cells.	Fura-2	16-22	transient receptor potential-like	Drosophila melanogaster	121-125
11152740-4	2001	Here, we used glutamate iontophoresis to induce Ca(2+) transients in hippocampal CA1 neurons injected with the Ca(2+)-indicator fura-2.	Fura-2	128-134	carbonic anhydrase 1	Homo sapiens	81-84
11136854-4	2001	In fura-2 assays, extracellular application of bacterial phosphatidylinositol (PI)-PLC or phosphatidylcholine (PC)-PLC caused a transient increase in TrpL channel activity, the magnitude of which was significantly less than that observed following receptor stimulation.	Fura-2	3-9	Phospholipase C at 21C	Drosophila melanogaster	83-86
11136854-4	2001	In fura-2 assays, extracellular application of bacterial phosphatidylinositol (PI)-PLC or phosphatidylcholine (PC)-PLC caused a transient increase in TrpL channel activity, the magnitude of which was significantly less than that observed following receptor stimulation.	Fura-2	3-9	Phospholipase C at 21C	Drosophila melanogaster	115-118
11136854-4	2001	In fura-2 assays, extracellular application of bacterial phosphatidylinositol (PI)-PLC or phosphatidylcholine (PC)-PLC caused a transient increase in TrpL channel activity, the magnitude of which was significantly less than that observed following receptor stimulation.	Fura-2	3-9	transient receptor potential-like	Drosophila melanogaster	150-154
11139441-1	2000	Using a method employing front-surface fura-2 fluorometry to measure the cytosolic Ca(2+) concentration, [Ca(2+)](i), the mechanism of endothelium-dependent regulation of vascular tone by thrombin was studied in porcine renal interlobar arterial strips.	Fura-2	39-45	coagulation factor II, thrombin	Homo sapiens	188-196
11135617-5	2001	Moreover, the treatment with recombinant t-PA leads to a 37% increase in NMDA-stimulated fura-2 fluorescence, which may reflect an increased NMDA-receptor function.	Fura-2	89-95	plasminogen activator, tissue type	Homo sapiens	41-45
11440808-7	2001	Consistent with abrogation of excitotoxic pathways, fura-2 imaging showed selective deficits in gp120-dependent intracellular Ca2+ responses in ethanol-pretreated slices.	Fura-2	52-58	inter-alpha-trypsin inhibitor heavy chain 4	Homo sapiens	96-101
11145120-5	2000	The intracellular Ca2+ concentration ([Ca2+]i) increased in fura-2-loaded ZLC islets following stimulation with 12 mmol/L glucose alone or GLP-1 in the presence of 12 mmol/L glucose.	Fura-2	60-66	glucagon	Rattus norvegicus	139-144
11179848-2	2000	In vitro, we noticed that the NMDA-evoked increase in Fura-2 detected intracellular Ca(2+) concentration in synaptoneurosomes from the rat, but not from the rabbit hippocampus, was strongly inhibited by the ryanodine receptor (RyR) antagonists dantrolene and ryanodine.	Fura-2	54-60	LOC100009439	Oryctolagus cuniculus	207-225
11179848-2	2000	In vitro, we noticed that the NMDA-evoked increase in Fura-2 detected intracellular Ca(2+) concentration in synaptoneurosomes from the rat, but not from the rabbit hippocampus, was strongly inhibited by the ryanodine receptor (RyR) antagonists dantrolene and ryanodine.	Fura-2	54-60	LOC100009439	Oryctolagus cuniculus	227-230
11032778-2	2000	In this study, spectrofluorimetric experiments in free solution, using Fluo-3 and Fura-2, showed that Zn2+ and Cd2+ enhanced the probe signal, Cu2+ quenched it, and Hg2+ had no effect.	Fura-2	82-88	CD2 molecule	Homo sapiens	111-114
11063339-13	2000	The AT1R was functional since a significant increase in [Ca(i)] was observed in Fura-2 loaded day 10 cells when stimulated with AII (182%, P<0.0001).	Fura-2	80-86	angiotensin II receptor type 1	Homo sapiens	4-8
11063339-13	2000	The AT1R was functional since a significant increase in [Ca(i)] was observed in Fura-2 loaded day 10 cells when stimulated with AII (182%, P<0.0001).	Fura-2	80-86	angiotensinogen	Homo sapiens	128-131
11025441-5	2000	The function of RyR in Ca(2+) signaling was investigated using single-cell fura-2 imaging.	Fura-2	75-81	ryanodine receptor 1	Homo sapiens	16-19
11056101-5	2000	After washout of the alpha-ADR agonist, simultaneous measurement of [Ca(2+)](i) transients with fura 2 and myocyte shortening by video edge-detection showed that [Ca(2+)](i) amplitude and myocyte shortening were decreased in alpha-ADR-treated myocytes, and the time to peak and time from peak to 80% decline of both [Ca(2+)](i) and myocyte shortening were increased.	Fura-2	96-102	carbonic anhydrase 2	Rattus norvegicus	69-75
11027555-2	2000	Bradykinin (10 nM) and thapsigargin (1 microM) provoked large Ca(2+) influxes under fura-2/AM fluoroscopy.	Fura-2	84-90	kininogen 1	Homo sapiens	0-10
11032778-4	2000	Digital imaging of living mussel haemocytes loaded with Fura-2/AM or Fluo-3/AM showed that Hg2+, Cu2+ and Cd2+ induced a rise in probe fluorescence, whereas up to 200 microM Zn2+ had no effect.	Fura-2	56-62	CD2 molecule	Homo sapiens	106-109
11009559-1	2000	Angiotensin II (Ang II)-induced Ca(2+) signaling was studied in isolated rat renal arterioles using fura-2.	Fura-2	100-106	angiotensinogen	Rattus norvegicus	0-22
10928946-4	2000	Measuring [Ca(2+)](i) by imaging analysis of fura-2-loaded cells, we demonstrated that alpha-thrombin (3-180 U l(-1)) induced a superimposable dose-dependent calcium transient in ECs from both strains.	Fura-2	45-51	coagulation factor II	Rattus norvegicus	93-101
10851228-2	2000	Stimulation of the particulate guanylate cyclase by brain-type natriuretic peptide in fura-2-loaded cells caused a profound potentiation of the ATP-stimulated and thapsigargin-stimulated rise in cytosolic free Ca(2+).	Fura-2	86-92	natriuretic peptide B	Homo sapiens	52-82
10913015-2	2000	We determined whether the VCAM-1-induced hyperpolarization is sufficient to augment the increase in intracellular free calcium ([Ca(2+)](i)) produced by Ca(2+) store depletion with thapsigargin (TG) and readdition of external CaCl(2) in fura 2-loaded THP-1 monocytes.	Fura-2	237-243	vascular cell adhesion molecule 1	Homo sapiens	26-32
10919286-6	2000	Moreover, binding sites for endothelin-1 were increased, and the calcium response to the contractile agonist, determined in single fura-2-loaded cells, was enhanced.	Fura-2	131-137	endothelin 1	Rattus norvegicus	28-40
11360670-2	2000	METHODS: The mobilization of cytosolic-free calcium induced by thrombin in washed platelets was observed by Ca(2+)-sensitive fluorescent indicator, Fura-2 AM and time scan measurement.	Fura-2	148-154	prothrombin	Oryctolagus cuniculus	63-71
10896718-16	2000	In fura-2-loaded cells, perforated patch whole-cell recordings show that ATP and UTP evoked slow outward currents at -60 mV, concomitant with the rise in [Ca2+]i, in approximately 30 % of rat intracardiac neurones.	Fura-2	3-9	carbonic anhydrase 2	Rattus norvegicus	155-158
10837492-5	2000	Activation of G(q/11)-coupled receptors or receptor tyrosine kinases induced Mn(2+) entry in fura-2-loaded mTRPC4/5-expressing cells.	Fura-2	93-99	transient receptor potential cation channel, subfamily C, member 4	Mus musculus	107-113
10742292-2	2000	The increase in the cytosolic Ca(2+) concentration ([Ca(2+)](i)) following repetitive stimulation with ATP or sphingosylphosphorylcholine (SPC) in single porcine aortic smooth muscle cells was investigated using the Ca(2+) indicator, fura-2.	Fura-2	234-240	proline rich protein gene cluster	Homo sapiens	139-142
10859594-5	2000	Using excitation wavelengths of lambda 340 nm (Fura-2) and lambda 485 nm (DAF-2) we could show that thrombin induces an intracellular calcium increase and simultaneously a NO formation in endothelial cells which could be blocked by a NO synthase inhibitor.	Fura-2	47-53	coagulation factor II, thrombin	Homo sapiens	100-108
10835036-3	2000	In rat primary astrocytes, short-term stimulation with thrombin resulted in a single [Ca2+]i transient and a dose-dependent de- and resensitisation, as assessed by single-cell Ca2+ imaging of fura-2-loaded astrocytes.	Fura-2	192-198	coagulation factor II	Rattus norvegicus	55-63
10792362-3	2000	METHODS: [Ca2+]i responses to PAF were examined in human eosinophils using a microscopic fura-2 fluorescence-ratio imaging system.	Fura-2	89-95	PCNA clamp associated factor	Homo sapiens	30-33
10642570-11	2000	Using the Ca(2+)-sensitive fluorescent indicator dye, fura-2, and digital imaging videomicroscopy, we found that PTH-rp (1 microM) stimulated intracellular free calcium ion concentrations ([Ca(2+)](i)) in single porcine theca cells.	Fura-2	54-60	parathyroid hormone like hormone	Homo sapiens	113-119
10771106-2	2000	The effect of anti-calreticulin antibody on intracellular free calcium concentration [Ca(2+)]i was studied in cultured neurons using fura-2 based microfluorometry.	Fura-2	133-139	calreticulin	Homo sapiens	19-31
10696094-1	2000	The mechanism of endothelium-dependent regulation of vascular tone of bradykinin was investigated by simultaneously monitoring the changes in the cytosolic Ca(2+) concentration and the force of smooth muscle in fura-2-loaded strips of the porcine renal artery with endothelium.	Fura-2	211-217	kininogen 1	Homo sapiens	70-80
10681455-9	2000	Among the thrombin concentrations tested, 50 pM induced intracellular Ca(2+) spikes in fura-2-loaded CA1 neurons whereas higher concentrations caused a sustained Ca(2+) elevation.	Fura-2	87-93	coagulation factor II	Rattus norvegicus	10-18
10681455-9	2000	Among the thrombin concentrations tested, 50 pM induced intracellular Ca(2+) spikes in fura-2-loaded CA1 neurons whereas higher concentrations caused a sustained Ca(2+) elevation.	Fura-2	87-93	carbonic anhydrase 1	Rattus norvegicus	101-104
10686291-1	2000	The regulatory mechanism of thrombin receptor responsiveness in in situ endothelial cells was investigated by evaluating elevations of cytosolic Ca(2+) concentration ([Ca(2+)](i)) in fura-2-loaded porcine aortic valvular strips.	Fura-2	183-189	coagulation factor II, thrombin	Homo sapiens	28-36
10704748-1	2000	The effects of the pro-inflammatory cytokine interleukin-1-beta (IL-1beta) on levels of intracellular calcium [Ca(2+)](i) in cultured human microglia have been studied using the fluorescent Ca(2+) indicator fura-2.	Fura-2	207-213	interleukin 1 beta	Homo sapiens	65-73
10670838-3	2000	Circulating PMN were isolated during 12 days, followed by determination of formyl-methionyl-leucyl-phenylalanine (fMLP)-induced PMN-superoxide production (PMN-SOP) by SOD-inhibitable ferricytochrome C reduction, and PMN cytosolic Ca2+ concentration ([Ca2+]i) by fluorescent fura2/AM (340/380 ratio).	Fura-2	274-279	formyl peptide receptor 1	Homo sapiens	114-118
10640725-3	2000	Using fura- 2 Ca2+ imaging of murine Th1 and Th2 clones, we observed that the Ca2+ rise elicited following store depletion with thapsigargin is significantly lower in Th2 cells than in Th1 cells.	Fura-2	6-13	negative elongation factor complex member C/D, Th1l	Mus musculus	37-40
10640725-3	2000	Using fura- 2 Ca2+ imaging of murine Th1 and Th2 clones, we observed that the Ca2+ rise elicited following store depletion with thapsigargin is significantly lower in Th2 cells than in Th1 cells.	Fura-2	6-13	heart and neural crest derivatives expressed 2	Mus musculus	167-170
10515989-4	1999	We investigated postnatal development of intracellular calcium dynamics in the proximal apical dendrites of CA1 pyramidal cells by whole cell patch-clamp recordings and calcium imaging with the Ca(2+) indicator fura-2.	Fura-2	211-217	carbonic anhydrase 1	Rattus norvegicus	108-111
10616806-6	2000	Functional significance of thrombin receptors was indicated by changes in [Ca2+]i in response to thrombin, as measured by FURA-2 fluorescence in RBCE cells.	Fura-2	122-128	coagulation factor II	Rattus norvegicus	27-35
10616806-6	2000	Functional significance of thrombin receptors was indicated by changes in [Ca2+]i in response to thrombin, as measured by FURA-2 fluorescence in RBCE cells.	Fura-2	122-128	coagulation factor II	Rattus norvegicus	97-105
10620186-10	2000	Confocal [Ca2+]i measurements using fura-2 or fluo-3 or fluo-4 on the single cell level show that some of the Ang II-mediated [Ca2+]i response originated from podocytes.	Fura-2	36-42	angiotensinogen	Rattus norvegicus	110-116
10564232-1	1999	Bradykinin (BK)-induced changes in intracellular calcium level ([Ca(2+)](i)) were studied on fura 2-loaded afferent (AA) and efferent glomerular arterioles (EA) microdissected from juxtamedullary renal cortex.	Fura-2	93-99	kininogen 1	Homo sapiens	0-10
10564232-1	1999	Bradykinin (BK)-induced changes in intracellular calcium level ([Ca(2+)](i)) were studied on fura 2-loaded afferent (AA) and efferent glomerular arterioles (EA) microdissected from juxtamedullary renal cortex.	Fura-2	93-99	kininogen 1	Homo sapiens	12-14
10532691-4	1999	Furthermore, ET-1 stimulated protein synthesis (maximum 73 +/- 32% increase in [3H]leucine incorporation by 10(-7) M (P < 0.05)), as well as triggering intracellular calcium transients in human cSMC, as visualised under fura-2 fluorescence microscopy.	Fura-2	223-229	endothelin 1	Homo sapiens	13-17
10799769-10	2000	Competitive receptor binding techniques using brain tissue sections as well as a fura-2 loaded primary cell culture system of the subfornical organ and organum vasculosum laminae terminalis demonstrate glial expression of functional ET(A) and ET(B) receptors, with calcium as intracellular messenger emerging primarily from intracellular stores.	Fura-2	81-87	endothelin receptor type A	Rattus norvegicus	233-238
11061984-3	2000	Ca(2+)fluxes in FMLP-activated neutrophils were measured using a fura-2/AM spectrofluorimetric procedure, while a colourimetric method was used to measure release of the primary granule enzyme, elastase, from the cells.	Fura-2	65-71	formyl peptide receptor 1	Homo sapiens	16-20
10601493-7	1999	Fura-2 measurements revealed a biphasic increase in [Ca2+]i when 200 microM ACPD was bath applied to cultured hippocampal neurones.	Fura-2	0-6	carbonic anhydrase 2	Rattus norvegicus	53-56
10516641-2	1999	The novel antimigraineur, dotarizine (30 microM), increased cytosolic Ca2+ concentration, [Ca2+]c, in fura-2-loaded bovine adrenal chromaffin cells.	Fura-2	102-108	carbonic anhydrase 2	Bos taurus	70-73
10535385-6	1999	Experiments performed on cytochalasin D-treated cells using Mn2+ as an indicator of Ca2+ influx quenched fura-2 fluorescence signals following vasopressin administration.	Fura-2	105-111	arginine vasopressin	Rattus norvegicus	143-154
10629863-1	1999	To clarify Ca2+ entry channels involved in the endothelin-1 (ET-1)-induced increase in the intracellular concentration ([Ca2+]i), we performed whole-cell recordings of patch-clamp techniques and monitoring of [Ca2+]i with Ca2+ indicators fura-2 and fluo-3 in A7r5 cells (a cell line derived from rat thoracic aortic smooth muscle cells).	Fura-2	238-244	endothelin 1	Rattus norvegicus	47-59
10629863-1	1999	To clarify Ca2+ entry channels involved in the endothelin-1 (ET-1)-induced increase in the intracellular concentration ([Ca2+]i), we performed whole-cell recordings of patch-clamp techniques and monitoring of [Ca2+]i with Ca2+ indicators fura-2 and fluo-3 in A7r5 cells (a cell line derived from rat thoracic aortic smooth muscle cells).	Fura-2	238-244	endothelin 1	Rattus norvegicus	61-65
10513576-2	1999	Ni2+ (1 mM) significantly inhibited the transient increase in intracellular Ca2+ concentration ([Ca2+]i) induced by Ang II (100 nM) in aortic smooth muscle cells, as measured using fura-2.	Fura-2	181-187	angiotensinogen	Rattus norvegicus	116-122
10509892-1	1999	The calcium responses to serotonin and thrombin, as assessed using the fluorescent indicator Fura-2, have been investigated in platelets taken from 59 non-smokers and 17 smokers.	Fura-2	93-99	coagulation factor II, thrombin	Homo sapiens	39-47
10628349-3	1999	The use of various spectroscopic methods (fluorescence spectroscopy with Fura-2/AM on viable cells, 13C NMR spectroscopy on lipid extracts) reveals osmotic and metabolic changes in HePC treated sensitive cells.	Fura-2	73-79	hepcidin antimicrobial peptide	Homo sapiens	181-185
10510460-9	1999	4 E-1/E-2 (1 microM) increased [Ca2+]i in fura-2 loaded CHOmu cell suspensions in a thapsigargin sensitive and naloxone reversible manner.	Fura-2	42-48	small nucleolar RNA, H/ACA box 73A	Homo sapiens	2-9
10469354-2	1999	METHODS: We examined the distribution of aminopeptidase A (APA) activity by using a specific APA inhibitor and by immunostaining with an antirat kidney APA antibody, the Ang III-induced variations of [Ca2+]i by using fura-2 and the characterization of the receptor subtype involved in the response to Ang III in cortical thick ascending limb (CTAL).	Fura-2	217-223	glutamyl aminopeptidase	Rattus norvegicus	59-62
10370087-3	1999	In T84 cells grown on impermeable supports, BK stimulated a transient increase in [Ca2+]i as assessed by fura-2 ratio imaging.	Fura-2	105-111	kininogen 1	Homo sapiens	44-46
10413074-6	1999	In cultured rat aortic smooth muscle cells loaded with fura-2, increases in intracellular free-Ca2+ concentration ([Ca2+]i) induced by angiotensin II were markedly inhibited in cells incubated with simvastatin and atorvastatin but not pravastatin.	Fura-2	55-61	angiotensinogen	Rattus norvegicus	135-149
10375648-1	1999	To understand the mechanism(s) underlying the Cd2+- and Co2+-induced increases in the cytosolic free Ca2+ concentration ([Ca]i) in cat adrenal chromaffin cells, we used nystatin-perforated patch recording method and fura-2 microfluorometry.	Fura-2	216-222	CD2 molecule	Canis lupus familiaris	46-49
10375648-5	1999	The Cd2+-induced inward current was activated in a concentration-dependent manner with a half-maximum concentration of 9.3x10(-8) M. The Cd2+- and Co2+-induced [Ca]i increases measured with fura-2 microfluorometry were maximal at 10(-6) and 10(-5) M, respectively, and the higher concentrations of both cations caused the smaller responses.	Fura-2	190-196	CD2 molecule	Canis lupus familiaris	4-7
10375648-5	1999	The Cd2+-induced inward current was activated in a concentration-dependent manner with a half-maximum concentration of 9.3x10(-8) M. The Cd2+- and Co2+-induced [Ca]i increases measured with fura-2 microfluorometry were maximal at 10(-6) and 10(-5) M, respectively, and the higher concentrations of both cations caused the smaller responses.	Fura-2	190-196	CD2 molecule	Canis lupus familiaris	137-140
10336664-1	1999	The properties of the vestibular Na+-Ca2+ exchanger in mammalian type I vestibular sensory cells were studied using fura-2 fluorescence and immunocytochemical techniques.	Fura-2	116-122	nascent polypeptide associated complex subunit alpha 2	Homo sapiens	33-40
10340744-1	1999	The effect of chemical anoxia (azide) in the presence of glucose on the free intracellular Ca2+ concentration ([Ca2+]i) and intracellular pH (pHi) in mouse neocortical neurons was investigated using Fura-2 and BCECF.	Fura-2	199-205	glucose-6-phosphate isomerase 1	Mus musculus	142-145
10320025-6	1999	In Fura-2-loaded intracardiac neurones, Met-enkephalin evoked a transient increase in [Ca2+]i.	Fura-2	3-9	proopiomelanocortin	Homo sapiens	40-54
10209009-4	1999	Effects of bradykinin and ramiprilat on intracellular signaling were determined by monitoring the activation of the extracellularly regulated kinases Erk1 and Erk2 as well as [Ca2+]i increases in fura 2-loaded endothelial cells.	Fura-2	196-202	kininogen 1	Homo sapiens	11-21
10217297-1	1999	The influx of the toxic cation Cd2+ was studied in fura 2-loaded rat cerebellar granule neurons.	Fura-2	51-57	Cd2 molecule	Rattus norvegicus	31-34
10191330-6	1999	Digital-imaging physiological studies with fura-2 detected a rise in intracellular calcium in response to hypocretin in cultured rat spinal cord neurons, indicating that spinal cord neurons express hypocretin-responsive receptors.	Fura-2	43-49	hypocretin	Mus musculus	106-116
10191330-6	1999	Digital-imaging physiological studies with fura-2 detected a rise in intracellular calcium in response to hypocretin in cultured rat spinal cord neurons, indicating that spinal cord neurons express hypocretin-responsive receptors.	Fura-2	43-49	hypocretin	Mus musculus	198-208
10098511-12	1999	[Ca2+]i moderately augmented the response to hCG in fura-2/AM-loaded mLTC-1 cells within 30-40 sec, reaching a plateau within 1-3 min.	Fura-2	52-58	hypertrichosis 2 (generalised, congenital)	Homo sapiens	45-48
10201687-3	1999	METHODS: Changes in [Ca2+]i were measured using fura-2 fluorescence spectroscopy in rat cortical brain slices at 90, 185, 370, and 705 microM isoflurane.	Fura-2	48-54	carbonic anhydrase 2	Rattus norvegicus	21-24
10082975-0	1999	Measurements of [Ca2+] using fura-2 in glioma C6 cells expressing calretinin with GFP as a marker of transfection: no Ca2+-buffering provided by calretinin.	Fura-2	29-35	calbindin 2	Homo sapiens	66-76
10066929-2	1999	The Ca2+-sensitive fluorescent indicator rhod-2 was used to measure mitochondrial [Ca2+] ([Ca2+]m) in single smooth muscle cells from the rat pulmonary artery, while simultaneously monitoring cytosolic [Ca2+] ([Ca2+]i) with fura-2.	Fura-2	224-230	carbonic anhydrase 2	Rattus norvegicus	4-7
10100864-1	1999	Caffeine mobilized an intracellular Ca2+ pool in intact fura-2-loaded INS-1 cells in suspension exposed to high (16 mM) [glucose], while a minor effect was observed with low (2 mM) [glucose].	Fura-2	56-62	insulin 1	Rattus norvegicus	70-75
10188626-1	1999	The elevation of intracellular [Ca2+] induced by bradykinin (Bk) was monitored with fura-2 fluorescence in human skin fibroblasts.	Fura-2	84-90	kininogen 1	Homo sapiens	49-59
10226172-10	1999	In fura-2-loaded pancreatic acinar cells Ins or Glu evoked small increases in [Ca2+]i compared with a much larger elevation with CCK-8.	Fura-2	3-9	cholecystokinin	Rattus norvegicus	129-132
10090417-4	1999	The mechanisms of angiotensin II-evoked calcium signaling were examined using fura-2-based fluorescent digital microscopy.	Fura-2	78-84	angiotensinogen	Rattus norvegicus	18-32
10188626-1	1999	The elevation of intracellular [Ca2+] induced by bradykinin (Bk) was monitored with fura-2 fluorescence in human skin fibroblasts.	Fura-2	84-90	kininogen 1	Homo sapiens	61-63
9852560-8	1998	Fura-2 recordings showed that BDNF elicited an increase in intracellular calcium concentration ([Ca2+]c).	Fura-2	0-6	brain derived neurotrophic factor	Homo sapiens	30-34
9930728-7	1999	PGE2 and the EP1 agonist 17-phenyl trinor PGE2 but not the EP3 agonist sulprostone elicited reversible intracellular [Ca2+] increases in microglia as measured by fura-2.	Fura-2	162-168	prostaglandin E receptor 1	Rattus norvegicus	13-16
10051189-2	1999	In the present report, changes in the intracellular Ca2+ concentration in the hippocampal CA1 area during transient global ischemia and reperfusion were measured using in vivo Ca2+ fluorometry with fura-2 in the four-vessel occlusion and reperfusion model in halothane-anesthetized rats.	Fura-2	198-204	carbonic anhydrase 2	Rattus norvegicus	52-55
10051189-2	1999	In the present report, changes in the intracellular Ca2+ concentration in the hippocampal CA1 area during transient global ischemia and reperfusion were measured using in vivo Ca2+ fluorometry with fura-2 in the four-vessel occlusion and reperfusion model in halothane-anesthetized rats.	Fura-2	198-204	carbonic anhydrase 1	Rattus norvegicus	90-93
10051189-2	1999	In the present report, changes in the intracellular Ca2+ concentration in the hippocampal CA1 area during transient global ischemia and reperfusion were measured using in vivo Ca2+ fluorometry with fura-2 in the four-vessel occlusion and reperfusion model in halothane-anesthetized rats.	Fura-2	198-204	carbonic anhydrase 2	Rattus norvegicus	176-179
10064008-5	1999	ATP and alpha,beta-methylene ATP, a specific agonist for P2X1 receptors, at micromolar concentrations also increased Mn2+ quench, but to lesser extents than did ADP, in SPD platelets isolated and loaded with fura-2 in the presence of apyrase.	Fura-2	208-214	purinergic receptor P2X 1	Homo sapiens	57-61
10064008-3	1999	With thrombin, this enhanced [Ca2+]i decay was associated with decreased Ca2+ influx, as measured by Mn2+ quench of fura-2 fluorescence.	Fura-2	116-122	coagulation factor II, thrombin	Homo sapiens	5-13
10211022-4	1998	Concentrations of [Ca2+]i in the pyramidal cell layer of the CA1 region were measured using a calcium indicator dye, fura-2.	Fura-2	117-123	carbonic anhydrase 2	Rattus norvegicus	19-22
9837897-6	1998	Analysis of the properties of recombinant RyR2 and RyR1 by measurement of intracellular Fura-2 fluorescence revealed that the different properties of RyR2 and RyR1 are retained in the recombinant expressed proteins.	Fura-2	88-94	ryanodine receptor 2	Oryctolagus cuniculus	42-46
9837897-6	1998	Analysis of the properties of recombinant RyR2 and RyR1 by measurement of intracellular Fura-2 fluorescence revealed that the different properties of RyR2 and RyR1 are retained in the recombinant expressed proteins.	Fura-2	88-94	ryanodine receptor 1	Oryctolagus cuniculus	51-55
9837897-6	1998	Analysis of the properties of recombinant RyR2 and RyR1 by measurement of intracellular Fura-2 fluorescence revealed that the different properties of RyR2 and RyR1 are retained in the recombinant expressed proteins.	Fura-2	88-94	ryanodine receptor 1	Oryctolagus cuniculus	159-163
9832138-5	1998	We found that fura-2 was useful in measuring small increases in [Zn2+]i associated with exposure to Zn2+ alone that may be mediated by a Na+/Ca2+ exchanger.	Fura-2	14-20	solute carrier family 8 member A1	Homo sapiens	137-155
9808762-2	1998	Immunoblot analysis revealed that A7r5 vascular smooth muscle cells expressed conventional (alpha), novel (delta and epsilon), and atypical (iota/lambda and mu) isoforms of protein kinase C. Stimulation of fura-2-loaded cells with 20 nM vasopressin induced a rapid transient increase in the intracellular concentration of calcium that was followed by a slowly declining component which was above baseline throughout the period of observation.	Fura-2	206-212	arginine vasopressin	Rattus norvegicus	237-248
9826729-3	1998	Fura-2-based Ca imaging showed that numerous chemokines, including SDF-1alpha, RANTES, and fractalkine, affect neuronal Ca signaling, suggesting that hippocampal neurons possess a wide variety of chemokine receptors.	Fura-2	0-6	C-C motif chemokine ligand 5	Homo sapiens	79-85
9826729-3	1998	Fura-2-based Ca imaging showed that numerous chemokines, including SDF-1alpha, RANTES, and fractalkine, affect neuronal Ca signaling, suggesting that hippocampal neurons possess a wide variety of chemokine receptors.	Fura-2	0-6	C-X3-C motif chemokine ligand 1	Homo sapiens	91-102
9804623-8	1998	By using the fura-2 fluorescence method, a difference in the kinetics of Ca2+ flux with Edg2 and Edg4 was observed.	Fura-2	13-19	lysophosphatidic acid receptor 1	Homo sapiens	88-92
9813161-2	1998	We measured the activity of nitrendipine-sensitive Ca2+ channels of intact cardiac myocytes in the presence of IGF1 by monitoring unidirectional Mn2+ influx measured as the quench of cytosolic fura-2 in electrically stimulated or K+-depolarized cells.	Fura-2	193-199	insulin like growth factor 1	Homo sapiens	111-115
9815040-10	1998	In fura 2-loaded, purified parietal cells, 10 min preincubation with IL-1beta dramatically reduced the initial transient peak elevation of intracellular Ca2+ concentration in response to carbachol.	Fura-2	3-9	interleukin 1 beta	Rattus norvegicus	69-77
9804623-8	1998	By using the fura-2 fluorescence method, a difference in the kinetics of Ca2+ flux with Edg2 and Edg4 was observed.	Fura-2	13-19	lysophosphatidic acid receptor 2	Homo sapiens	97-101
9742162-8	1998	Because NMDA receptor activation leads to increased intracellular Ca2+ concentration ([Ca2+]i), we studied the effect of the BDNF- and FGF2-induced reduction in NR2A and NR2C synthesis on the NMDA-evoked Ca2+ responses by single-cell fura-2 fluorescence ratio imaging.	Fura-2	234-240	fibroblast growth factor 2	Rattus norvegicus	135-139
9770374-4	1998	Free cytosolic Ca2+ was measured with the use of fluorescent dye Fura-2 during PLA2 agonist/antagonist studies.	Fura-2	65-71	phospholipase A2 group IB	Homo sapiens	79-83
9849678-2	1998	Simultaneous measurements of kainate-activated Ca2+ fluxes and inward currents, using fura-2 microfluorimetry under voltage clamp conditions, suggested the existence of GluR2 containing channels which are permeable to Ca2+ and insensitive to Joro spider toxin (JSTx).	Fura-2	86-92	glutamate ionotropic receptor AMPA type subunit 2	Homo sapiens	169-174
9767522-3	1998	METHODS: LPS- or BK-induced increments of intracellular Ca2+ were assessed by microspectrofluorimetry with fura-2 in passaged bovine aortic endothelial cells.	Fura-2	107-113	kininogen 1	Bos taurus	17-19
11717867-2	1998	METHODS: The fura-2 microfluorimetry was used to measure changes of [Ca2+]i in OHCs of the guinea pig cochlea after application of acetylcholine, ATP and carbacholine.	Fura-2	13-19	LOW QUALITY PROTEIN: carbonic anhydrase 2	Cavia porcellus	69-72
9920348-0	1998	Changes in cytosolic Ca2+ measured by use of fura-2 and contraction produced by quick stretch and various stimulants in canine cerebral artery.	Fura-2	45-51	carbonic anhydrase 2	Canis lupus familiaris	21-24
9873837-7	1998	In order to identify what triggers the PLA2 activation, [Ca2+]i was measured by using a fluorescence dye, Fura-2.	Fura-2	106-112	phospholipase A2 group IB	Canis lupus familiaris	39-43
9736645-4	1998	Fura-2 fixation combined with immunohistochemistry revealed that fura-2-loaded cells were GFAP+/O4(-) and were therefore a population of neonatal white matter astrocytes.	Fura-2	0-6	glial fibrillary acidic protein	Rattus norvegicus	90-94
9714858-4	1998	Ratio imaging with fura-2 revealed moderate dendritic [Ca2+] increases (approximately 500 nM) during only the initial approximately 30 s of the 7.5 min stimulation period.	Fura-2	19-25	carbonic anhydrase 2	Rattus norvegicus	55-58
9736645-4	1998	Fura-2 fixation combined with immunohistochemistry revealed that fura-2-loaded cells were GFAP+/O4(-) and were therefore a population of neonatal white matter astrocytes.	Fura-2	65-71	glial fibrillary acidic protein	Rattus norvegicus	90-94
9694886-2	1998	In this study, we examined the role of PKC in regulating the coupling of the CaR to Ca2+i dynamics in fura-2-loaded human embryonic kidney cells (HEK293 cells) transiently transfected with the human parathyroid CaR.	Fura-2	102-108	proline rich transmembrane protein 2	Homo sapiens	39-42
9760037-2	1998	Bradykinin evoked a [Ca2+]i transient in a dose-dependent manner, measured by fura-2 fluorimetry and digital video imaging.	Fura-2	78-84	kininogen 1	Canis lupus familiaris	0-10
9679168-6	1998	The measurements of changes in pHi evoked by changes in pHo were employed in subsequent experiments to correct for the effects of changes in pHi on the Kd of fura-2 for Ca2+.	Fura-2	158-164	glucose-6-phosphate isomerase	Rattus norvegicus	141-144
9685362-7	1998	Only phospholipase C-beta1 is involved in the mobilization of Ca2+ from the endoplasmic reticulum of Fura-2-loaded confluent OHS-4 cells and the formation of inositol 1,4,5-trisphosphate by CGRP; PLC-gamma have no effect.	Fura-2	101-107	phospholipase C beta 1	Homo sapiens	5-26
9685362-7	1998	Only phospholipase C-beta1 is involved in the mobilization of Ca2+ from the endoplasmic reticulum of Fura-2-loaded confluent OHS-4 cells and the formation of inositol 1,4,5-trisphosphate by CGRP; PLC-gamma have no effect.	Fura-2	101-107	calcitonin related polypeptide alpha	Homo sapiens	190-194
9687496-6	1998	Ca2+ store depletion with thapsigargin induced TRP5-mediated calcium entry dependent on the concentration of extracellular calcium, as seen by dual wavelength fura-2 fluorescence ratio measurements.	Fura-2	159-165	carbonic anhydrase 2	Homo sapiens	0-3
9687496-6	1998	Ca2+ store depletion with thapsigargin induced TRP5-mediated calcium entry dependent on the concentration of extracellular calcium, as seen by dual wavelength fura-2 fluorescence ratio measurements.	Fura-2	159-165	transient receptor potential cation channel subfamily C member 5	Homo sapiens	47-51
9722316-2	1998	Using fura-2-loaded platelets from 51 healthy volunteers, high variability was detected in the Ca2+ responses evoked by the receptor agonists, thrombin and collagen, and the inhibitor of sarco/endoplasmic reticulum Ca2+-ATPases (SERCA), thapsigargin (Tg).	Fura-2	6-12	coagulation factor II, thrombin	Homo sapiens	143-151
9793689-1	1998	In human U373 MG astrocytoma cells, histamine and substance P stimulated similar peak increases in intracellular free calcium concentrations ([Ca2+]i), as measured by single cell imaging of Fura-2 fluorescence.	Fura-2	190-196	tachykinin precursor 1	Homo sapiens	50-61
9749756-6	1998	In single-cell Fura-2 measurements, bFGF elicited a long lasting rise of the cytosolic calcium concentration that was dependent on [Ca2+]o.	Fura-2	15-21	fibroblast growth factor 2	Gallus gallus	36-40
9871763-2	1998	The hNK3 ligands 3 antagonise the calcium mobilisation caused by activation of hNK3 receptors expressed in CHO cells as measured using fura-2 microspectrofluorimetry.	Fura-2	135-141	NK3 homeobox 1	Homo sapiens	4-8
9698207-1	1998	The mechanism of thrombin-induced endothelium-dependent relaxation was investigated using fura-2 front-surface fluorometry.	Fura-2	90-96	coagulation factor II, thrombin	Sus scrofa	17-25
9871763-2	1998	The hNK3 ligands 3 antagonise the calcium mobilisation caused by activation of hNK3 receptors expressed in CHO cells as measured using fura-2 microspectrofluorimetry.	Fura-2	135-141	NK3 homeobox 1	Homo sapiens	79-83
9689597-2	1998	To characterize signal transduction mechanisms of Ang II, we examined changes in intracellular Ca2+ concentration ([Ca2+]i) of fura-2-loaded cultured adult rat cardiac fibroblasts by fluorescence photometry.	Fura-2	127-133	angiotensinogen	Rattus norvegicus	50-56
9720762-1	1998	In fura-2-labelled human platelets, the thiol oxidising agent diamide decreases the intracellular calcium response to thrombin and serotonin without affecting the basal calcium levels.	Fura-2	3-9	coagulation factor II, thrombin	Homo sapiens	118-126
9519747-4	1998	Using the Fura-2 technique, application of either 1 nmol/l bradykinin or 1 micromol/l ATP to the normoglycemic endothelial cells led to a significant increase in intracellular calcium, whereas the hyperglycemic cells showed significantly less reactivity to both agents.	Fura-2	10-16	kininogen 1	Homo sapiens	59-69
9610724-4	1998	Using the fluorescent probe Fura-2, we assayed the cytoplasmic calcium levels in human cancer cells stimulated with anti-Trop-2 or control antibodies.	Fura-2	28-34	tumor associated calcium signal transducer 2	Homo sapiens	121-127
9494083-2	1998	Rates of Ca2+ inflow were estimated by measuring the increase in the fluorescence of intracellular fura-2 following the addition of extracellular Ca2+ (Ca2+o) to cells incubated in the absence of added Ca2+o.	Fura-2	99-105	carbonic anhydrase 2	Rattus norvegicus	9-12
9494083-2	1998	Rates of Ca2+ inflow were estimated by measuring the increase in the fluorescence of intracellular fura-2 following the addition of extracellular Ca2+ (Ca2+o) to cells incubated in the absence of added Ca2+o.	Fura-2	99-105	carbonic anhydrase 2	Rattus norvegicus	146-149
9494083-2	1998	Rates of Ca2+ inflow were estimated by measuring the increase in the fluorescence of intracellular fura-2 following the addition of extracellular Ca2+ (Ca2+o) to cells incubated in the absence of added Ca2+o.	Fura-2	99-105	carbonic anhydrase 2	Rattus norvegicus	146-149
9494083-2	1998	Rates of Ca2+ inflow were estimated by measuring the increase in the fluorescence of intracellular fura-2 following the addition of extracellular Ca2+ (Ca2+o) to cells incubated in the absence of added Ca2+o.	Fura-2	99-105	carbonic anhydrase 2	Rattus norvegicus	146-149
9461611-7	1998	Analysis of NCKX2 function by fluorescent imaging of fura-2-loaded transfected cells demonstrated that NCKX2 is a potassium-dependent sodium/calcium exchanger.	Fura-2	53-59	solute carrier family 24 member 2	Rattus norvegicus	12-17
9582501-7	1998	In fura-2 loaded BAG cells, BK (100 nM) induced a typical biphasic Ca2+ response composed of a rapid and transient increase of intracellular Ca2+ concentration [Ca2+]i which slowly declined to a level that remained above basal level for about 5 min.	Fura-2	3-9	kininogen 1	Bos taurus	28-30
9464995-3	1998	Extracellularly applied ATP (50 microM) evoked fast [Ca2+]i rises revealed by a rapid and transient increase in fura-2 F340/F380 ratio in all Purkinje cells tested, whereas granule cells failed to show a response to ATP.	Fura-2	112-118	carbonic anhydrase 2	Rattus norvegicus	53-56
9461611-7	1998	Analysis of NCKX2 function by fluorescent imaging of fura-2-loaded transfected cells demonstrated that NCKX2 is a potassium-dependent sodium/calcium exchanger.	Fura-2	53-59	solute carrier family 24 member 2	Rattus norvegicus	103-108
9502114-4	1998	Measurement of intracellular Ca2+ concentration ([Ca2+]i) by Fura-2 indicates that VIP gradually elevates [Ca2+]i, with the maximum level attained at 4 min following hormone addition.	Fura-2	61-67	vasoactive intestinal peptide	Rattus norvegicus	83-86
9417057-8	1998	Measurement of intracellular Ca2+ concentrations in Fura2-loaded cells showed that cell lines expressing hTrp3 have significantly higher basal and agonist-stimulated influxes of Ca2+, Mn2+, Ba2+, and Sr2+ than control cells.	Fura-2	52-57	transient receptor potential cation channel subfamily C member 3	Homo sapiens	105-110
9490829-19	1998	The rate of Mn2+ quench of fura-2 fluorescence was increased by the inhibitors, and the inhibitors induced a small inward current when K+ channels were blocked that preceded the rise in [Ca2+]i.	Fura-2	27-33	carbonic anhydrase 2	Rattus norvegicus	187-190
9490842-2	1998	By applying fura-2-based fluorometric calcium imaging to neonatal rat hippocampal slices we identified a developmentally regulated spontaneous neuronal activity in the CA1 region of the hippocampus.	Fura-2	12-18	carbonic anhydrase 1	Rattus norvegicus	168-171
9481674-9	1998	The peak of the Ca2+ signal measured with fura-2 preceded peak 1 of force and then declined to a lower suprabasal steady-state level.	Fura-2	42-48	pseudopodium-enriched atypical kinase 1	Cavia porcellus	58-64
9649922-7	1998	Transient increases in intracellular calcium concentration in cSMCs in response to angiotensin II stimulation were visualized under fura-2 fluorescence microscopy.	Fura-2	132-138	angiotensinogen	Homo sapiens	83-97
9421413-2	1998	In these cells, insulin evoked the elevation of cytoplasmic free calcium concentration, [Ca2+]c, measured by using fura-2.	Fura-2	115-121	insulin	Cricetulus griseus	16-23
9441706-1	1997	Regulation of intracellular free Ca2+ concentration ([Ca2+]i) by an Na+/Ca2+ exchanger was studied in cultures of human retinal pigment epithelial cells using Ca(2+)-indicator dyes (fura-2 and fluo-3) and digital fluorescence imaging.	Fura-2	182-188	solute carrier family 8 member A1	Homo sapiens	68-86
9418702-7	1997	RESULT(S): All oocytes loaded with fura-2 and then exposed to ionophore exhibited an isolated elevation of [Ca]i, followed by prompt return to baseline levels.	Fura-2	35-41	carbonic anhydrase 1	Homo sapiens	108-112
9444969-2	1998	In each strain the Ang II induced changes of [Ca2+]i were measured in cultured glomerular mesangial cells (MC) using the calcium-sensitive fluorescent dye, fura2.	Fura-2	156-161	angiotensinogen	Rattus norvegicus	19-25
9374730-3	1997	In human ASM cells loaded with fura 2, TNF-alpha and interleukin (IL)-1 beta significantly enhanced thrombin- and bradykinin-evoked elevations of intracellular Ca2+.	Fura-2	31-37	tumor necrosis factor	Homo sapiens	39-48
9423175-4	1997	PACAP release from islets was bioassayed using the response of cytosolic Ca2+ concentration ([Ca2+]i) in single beta-cells, monitored by dual-wavelength fura-2 microfluorometry.	Fura-2	153-159	adenylate cyclase activating polypeptide 1	Rattus norvegicus	0-5
9368034-7	1997	Transient expression of mTrp6 in COS.M6 cells by transfection of the full-length mTrp6 cDNA increases Ca2+ entry induced by stimulation of co-transfected M5 muscarinic acetylcholine receptor with carbachol (CCh), as seen by dual wavelength fura 2 fluorescence ratio measurements.	Fura-2	240-246	transient receptor potential cation channel, subfamily C, member 6	Mus musculus	24-29
9368034-7	1997	Transient expression of mTrp6 in COS.M6 cells by transfection of the full-length mTrp6 cDNA increases Ca2+ entry induced by stimulation of co-transfected M5 muscarinic acetylcholine receptor with carbachol (CCh), as seen by dual wavelength fura 2 fluorescence ratio measurements.	Fura-2	240-246	transient receptor potential cation channel, subfamily C, member 6	Mus musculus	81-86
9374730-3	1997	In human ASM cells loaded with fura 2, TNF-alpha and interleukin (IL)-1 beta significantly enhanced thrombin- and bradykinin-evoked elevations of intracellular Ca2+.	Fura-2	31-37	interleukin 1 beta	Homo sapiens	53-76
9374730-3	1997	In human ASM cells loaded with fura 2, TNF-alpha and interleukin (IL)-1 beta significantly enhanced thrombin- and bradykinin-evoked elevations of intracellular Ca2+.	Fura-2	31-37	coagulation factor II, thrombin	Homo sapiens	100-108
9374730-3	1997	In human ASM cells loaded with fura 2, TNF-alpha and interleukin (IL)-1 beta significantly enhanced thrombin- and bradykinin-evoked elevations of intracellular Ca2+.	Fura-2	31-37	kininogen 1	Homo sapiens	114-124
9481478-1	1997	The fluorescent indicator Fura-2 was used to characterize the store-operated Ca2+ entry in insulin-releasing pancreatic beta-cells.	Fura-2	26-32	insulin	Homo sapiens	91-98
9383042-4	1997	Using the ratiometric fluorescent indicator fura-2, we observed large spontaneous [Ca2+]i oscillations.	Fura-2	44-50	carbonic anhydrase 2	Homo sapiens	83-86
9353354-6	1997	In cells loaded with Fura-2, atrial natriuretic peptide both augmented depolarization-induced increases of intracellular free calcium concentrations and accelerated the depolarization-induced quenching of the Fura-2 signal by manganese, findings consistent with enhanced conductivity of calcium channels.	Fura-2	21-27	natriuretic peptide A	Rattus norvegicus	29-55
9353354-6	1997	In cells loaded with Fura-2, atrial natriuretic peptide both augmented depolarization-induced increases of intracellular free calcium concentrations and accelerated the depolarization-induced quenching of the Fura-2 signal by manganese, findings consistent with enhanced conductivity of calcium channels.	Fura-2	209-215	natriuretic peptide A	Rattus norvegicus	29-55
9357760-3	1997	CaR agonists (Ca2+, poly-L-arginine, protamine) mediated an increase in intracellular Ca2+ in HT-29-18-C1 cells (monitored by changes in fura 2 fluorescence), which was dependent on release from thapsigargin-sensitive stores.	Fura-2	137-143	calcium sensing receptor	Homo sapiens	0-3
9277485-6	1997	VEGF also increased the intracellular calcium concentration in endothelial cells of perfused microvessels as determined by measurement with fura 2.	Fura-2	140-146	vascular endothelial growth factor A	Homo sapiens	0-4
9344760-3	1997	Compared with hypothyroid animals, myocytes isolated from hyperthyroid rat hearts demonstrated a significant: (1) increase in extent of shortening; (2) decrease in the time to peak contraction; (3) increase in the peak amplitude of the fura-2 fluorescence ratio; (4) decrease in pHi (DeltapHi=0.	Fura-2	236-242	glucose-6-phosphate isomerase	Rattus norvegicus	279-282
9385466-0	1997	Calibration of Fura-2 signals introduces errors into measurement of thrombin-stimulated calcium mobilisation in human platelets.	Fura-2	15-21	coagulation factor II, thrombin	Homo sapiens	68-76
9385466-1	1997	The intracellular calcium indicator dye Fura-2 has been widely used for the study of human platelet thrombin receptor-mediated calcium mobilisation in disease states.	Fura-2	40-46	coagulation factor II, thrombin	Homo sapiens	100-108
9385466-3	1997	In the present study, it is found (a) that a greater peak response to thrombin is seen when 1 mumol/l Fura-2/AM rather than 2 or 3 mumol/l is used; and (b) that calibration leads to a poorer test-retest reliability and in general a greater variability of the obtained calcium signal than when the simple measurement of the 340 nm/380 nm fluorescence ratio is used.	Fura-2	102-108	coagulation factor II, thrombin	Homo sapiens	70-78
9281578-1	1997	Extracellular ATP (ATPo) elicits a robust change in the concentration of intracellular Ca2+ ([Ca2+]i) in fura-2-loaded mouse thymocytes.	Fura-2	105-111	ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit	Mus musculus	19-23
9316451-2	1997	Presently, we used single-cell Ca2+ imaging to study the combined effects of 1,25(OH)2D3 and parathyroid hormone (PTH) during generation of Ca2+ transients in fura 2-loaded MC3T3-E1 cells.	Fura-2	159-165	parathyroid hormone	Mus musculus	93-112
9277347-1	1997	Muscarinic stimulation of fura 2-loaded smooth muscle cells evoked a rapidly inactivating Ca(2+)-activated Cl- current [ICl(Ca)] and a sustained nonselective cation current (Icat) as well as a transient (delta Ca(tran)) and a sustained (delta Ca(sus)) elevation of cytosolic Ca2+ concentration ([Ca2+]i).	Fura-2	26-32	catenin beta interacting protein 1	Homo sapiens	174-178
9310305-1	1997	The intracellular free Ca2+ concentration ([Ca2+]i) in the CA1 pyramidal cell layer was measured using fura-2-loaded hippocampal slices prepared from adult rats.	Fura-2	103-109	carbonic anhydrase 2	Rattus norvegicus	23-26
9310305-1	1997	The intracellular free Ca2+ concentration ([Ca2+]i) in the CA1 pyramidal cell layer was measured using fura-2-loaded hippocampal slices prepared from adult rats.	Fura-2	103-109	carbonic anhydrase 2	Rattus norvegicus	44-47
9310305-1	1997	The intracellular free Ca2+ concentration ([Ca2+]i) in the CA1 pyramidal cell layer was measured using fura-2-loaded hippocampal slices prepared from adult rats.	Fura-2	103-109	carbonic anhydrase 1	Rattus norvegicus	59-62
9321915-12	1997	To investigate the effect of stimulation of the CaR on intracellular Ca2+ (Ca2+i), we used the acetoxymethyl ester of fura 2 to monitor the Ca2+i.	Fura-2	118-124	nuclear receptor subfamily 1, group I, member 3	Rattus norvegicus	48-51
9334884-1	1997	In Chinese hamster ovary cells stably expressing the cloned human cholecystokinin (CCK)B/ gastrin receptor, cholecystokinin octapeptide (CCK-8) evoked increases in [Ca2+]i monitored by digitized video imaging of fura-2 fluorescence ratios.	Fura-2	212-218	cholecystokinin B receptor	Homo sapiens	90-106
9277471-5	1997	Systolic fluorescence ratio using fura 2 as the Ca2+ indicator fell by 11.0%, and the decline of the fluorescence ratio was prolonged by 29.8%.	Fura-2	34-40	carbonic anhydrase 2	Rattus norvegicus	48-51
9277471-9	1997	The relaxation abnormalities are most likely caused by altered Ca2+ handling, since we observed a reduction in the sarcoplasmic reticulum Ca2+ content and a prolongation of the decline of the fura 2 transient.	Fura-2	192-198	carbonic anhydrase 2	Rattus norvegicus	63-66
9259111-5	1997	The free cytosolic Ca++ concentration, measured by Fura-2, increased after thrombin or TRAP stimulation in the cells expressing TR-1.	Fura-2	51-57	coagulation factor II	Rattus norvegicus	75-83
9263987-13	1997	In the next step, [Ca2+]i changes were measured in single cells loaded with Fura-2 after microinjection of neutralizing PKC isoenzyme-specific antibodies.	Fura-2	76-82	protein kinase C alpha	Bos taurus	120-123
9271330-6	1997	In fura-2-loaded platelets, the elevation of intracellular free calcium concentration stimulated by AA, thrombin, and 4-bromo-A23187 was inhibited by NQ-Y15 in a concentration-dependent manner.	Fura-2	3-9	coagulation factor II	Rattus norvegicus	104-112
9222898-4	1997	In whole cell clamped myocytes dialyzed with 0.1-0.2 mM Fura-2, the magnitude of ICa and Ca2+i-transient triggered by ICa or caffeine were not significantly different in transgenic vs. control myocytes.	Fura-2	56-62	inhibitor of carbonic anhydrase	Mus musculus	118-121
9246571-8	1997	AT (1 microM) increased HUVEC intracellular calcium mobilization, as monitored by FURA-2, to 245 nM from control (70 nM), however, pre-treatment with either BK or the bradykinin receptor antagonist decreased the AT induced intracellular calcium mobilization compared to AT alone.	Fura-2	82-88	kininogen 1	Homo sapiens	167-177
9222898-12	1997	In 0.1 mM Fura-2-dialyzed transgenic myocytes 100 microM Cd2+ effectively blocked ICa and suppressed Cai-transients at -10 or +50 mV.	Fura-2	10-16	inhibitor of carbonic anhydrase	Mus musculus	82-85
11596320-5	1997	Furthermore, Fura-2/AM assay was more reliable than MTT assay for screening MDR modulators because MTT assay was dependent on the viable cells, while Fura-2/AM assay was dependent on the function of P-gp.	Fura-2	13-19	phosphoglycolate phosphatase	Homo sapiens	199-203
11596320-5	1997	Furthermore, Fura-2/AM assay was more reliable than MTT assay for screening MDR modulators because MTT assay was dependent on the viable cells, while Fura-2/AM assay was dependent on the function of P-gp.	Fura-2	150-156	phosphoglycolate phosphatase	Homo sapiens	199-203
9174646-1	1997	We previously demonstrated that histamine and bradykinin evoke an increase in intracellular Ca2+ ([Ca2+]i) in human gingival fibroblasts by using a fluorescent Ca2+ indicator Fura-2.	Fura-2	175-181	kininogen 1	Homo sapiens	46-56
9134961-6	1997	The Fura-2 assay was used to estimate whether low dosages of exogenous PAF affect cultured neurons.	Fura-2	4-10	PCNA clamp associated factor	Rattus norvegicus	71-74
9178189-6	1997	Changes in intracellular calcium ion concentration ([Ca2+]i) induced by FMLP (30 nM) were studied in fura-2-loaded human PMN.	Fura-2	101-107	formyl peptide receptor 1	Homo sapiens	72-76
9136085-5	1997	In suspended cells loaded with the calcium indicator dye fura-2, pretreatment with PKC inhibitors calphostin C (100 nM x 1 h) and H-7 (30 microM x 18 h) potentiated the effects of 1 microgram/ml bPTH (1-84) on Cai2+ (83% increase over basal) by 1.4- and 1.65-fold, respectively.	Fura-2	57-63	protein kinase C, gamma	Rattus norvegicus	83-86
9134961-8	1997	After preincubation for 120 min, the Fura-2-loaded neurons were exposed to various concentrations of PAF for 60 min.	Fura-2	37-43	PCNA clamp associated factor	Rattus norvegicus	101-104
9134961-9	1997	By measuring the fluorescent intensity of the medium as representing the amount of Fura-2 released from damaged neurons, we detected an increased release of Fura-2, even at low doses of PAF (P < 0.01 at 10 nM PAF).	Fura-2	157-163	PCNA clamp associated factor	Rattus norvegicus	212-215
9017193-6	1997	The relationship between the [Ca2+]t measured with furaptra and the postsynaptic response was the same as previously observed with fura-2: the postsynaptic response was proportional to about the fourth power (m approximately 4) of the amplitude of either [Ca2+]t or calcium influx.	Fura-2	131-137	carbonic anhydrase 2	Homo sapiens	30-33
9045728-10	1997	We also show that Ca2+ influx into hippocampal neurons in response to cyclic nucleotide elevation can be detected using fura-2 imaging.	Fura-2	120-126	carbonic anhydrase 2	Rattus norvegicus	18-21
9071214-4	1997	Changes in intracellular [Ca2+] ([Ca2+]i) were measured on fura-2-loaded acini in response to VIP.	Fura-2	59-65	vasoactive intestinal peptide	Rattus norvegicus	94-97
9105731-1	1997	Ca2+ transients evoked by endothelin-1 (ET-1) were measured in single cells of an immortalized human tracheal epithelial cell line using Fura-2.	Fura-2	137-143	endothelin 1	Homo sapiens	40-44
9065736-2	1997	Using fura-2-loaded cells, we observed that the release induced by AVP occurred concurrently with calcium (Ca2+) mobilization from internal stores and entry of external Ca2+, whereas AlF4(-)-dependent arachidonate release was much slower and was not accompanied by intracellular Ca2+ mobilization.	Fura-2	6-12	arginine vasopressin	Rattus norvegicus	67-70
8986232-10	1996	HGF-induced changes in [Ca2+]i were measured in single fura 2-loaded hepatocytes by fluorescence imaging techniques.	Fura-2	55-61	hepatocyte growth factor	Rattus norvegicus	0-3
9056076-1	1997	In cultured endothelial cells, the temperature dependence of bradykinin-initiated Ca2+ signaling was studied using Fura-2 technique.	Fura-2	115-121	kininogen 1	Homo sapiens	61-71
9170163-7	1997	Acute addition of fibronectin to coverslips of uveal melanocytes loaded with Fura-2 produced an acute and transient increase in intracellular calcium which was more prevalent in low density than higher density cells.	Fura-2	77-83	fibronectin 1	Homo sapiens	18-29
10453550-3	1997	When verapamil, EG-TA, dantrolene, and Fura-2/AM, were added to the medium, the 3H-leucine incorporations stimulated by Ang II were reduced by 17%, 19%, 22%, and 27%.	Fura-2	39-45	angiotensinogen	Homo sapiens	120-126
9007535-2	1996	Using ratio imaging with fura-2, we demonstrate that the induction of this long-term depression is associated with a rapid and transient (approximately 30 s) dendritic Ca2+ increase (approximately 500 nM) dependent on the activation of voltage-gated Ca2+ channels.	Fura-2	25-31	carbonic anhydrase 2	Rattus norvegicus	168-171
9007535-2	1996	Using ratio imaging with fura-2, we demonstrate that the induction of this long-term depression is associated with a rapid and transient (approximately 30 s) dendritic Ca2+ increase (approximately 500 nM) dependent on the activation of voltage-gated Ca2+ channels.	Fura-2	25-31	carbonic anhydrase 2	Rattus norvegicus	250-253
8908321-4	1996	Problems of mutual interference of Ca2+ and Cd2+ analysis with the fluorescent probe Fura-2 could be overcome by the use of the fluorine 19 nuclear magnetic resonance (19F-NMR) probe acetoxymethyl ester of 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N"N"-tetraacetic acid (5F-BAPTA), which allows the measurement of free intracellular Ca2+, Cd2+ and other metal ions concurrently.	Fura-2	85-91	Cd2 molecule	Rattus norvegicus	44-47
8997399-1	1996	This study provides an initial characterization of basic morphological properties of cultures of vascular smooth muscle cells (VSMC) from rat preglomerular resistance vessels and of the functional coupling of angiotensin II (ANG II) receptors to cytosolic free calcium concentration ([Ca2+]i (fura 2 fluorescence photometry).	Fura-2	293-299	angiotensinogen	Rattus norvegicus	225-231
9010006-3	1996	Using the Ca2+ indicator dye fura-2 in a cell culture model, we found that TNF-alpha (10-1000 U ml-1), but not interleukin 1 or 6, induced a slow but more than two-fold increase of the intracellular Ca2+ concentration, which could be blocked by Co2+ (1.0 mM), verapamil (100 microM) or omission of external Ca2+.	Fura-2	29-35	tumor necrosis factor	Homo sapiens	75-84
8952695-3	1996	AII also triggered a fast accumulation of IP3 and a rapid increase in [Ca2+]i in cells loaded with the Ca(2+)-responsive fluorescent dye fura-2.	Fura-2	137-143	angiotensinogen	Homo sapiens	0-3
8941710-2	1996	alpha3/beta4-nAChR-expressing clones were identified using the fura-2 Ca2+ imaging technique, and were further characterised by single-cell and whole-cell patch-clamp studies.	Fura-2	63-69	tubulin beta 3 class III	Homo sapiens	0-12
8908321-4	1996	Problems of mutual interference of Ca2+ and Cd2+ analysis with the fluorescent probe Fura-2 could be overcome by the use of the fluorine 19 nuclear magnetic resonance (19F-NMR) probe acetoxymethyl ester of 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N"N"-tetraacetic acid (5F-BAPTA), which allows the measurement of free intracellular Ca2+, Cd2+ and other metal ions concurrently.	Fura-2	85-91	Cd2 molecule	Rattus norvegicus	340-343
8917448-2	1996	Therefore, we analyzed bradykinin-activated Ca2+ influx into human foreskin fibroblast cells, HF-15, by fura-2 and 45Ca labeling to discriminate between Ca2+ influx into the fura-sensitive compartment and Ca uptake into fura-insensitive Ca stores.	Fura-2	104-110	kininogen 1	Homo sapiens	23-33
8892650-5	1996	When fura-2-loaded cells were first exposed to RANTES, which failed to elevate the intracellular calcium concentration, the response to a subsequent challenge with eotaxin was inhibited in a dose-dependent manner.	Fura-2	5-11	C-C motif chemokine 5	Cavia porcellus	47-53
8892650-5	1996	When fura-2-loaded cells were first exposed to RANTES, which failed to elevate the intracellular calcium concentration, the response to a subsequent challenge with eotaxin was inhibited in a dose-dependent manner.	Fura-2	5-11	eotaxin	Cavia porcellus	164-171
8914016-2	1996	The AVP-induced cellular signal transduction, including inositol 1,4,5-trisphosphate (IP3) production, fura-2 intracellular calcium measurements and cellular alkalinization, was significantly greater in cells of SHR than those of WKY.	Fura-2	103-109	arginine vasopressin	Rattus norvegicus	4-7
21594530-2	1996	Bradykinin stimulated an increase in [Ca2+](i), (monitored by the fura-2 fluorescence) in fibroblasts obtained from both the skin of a normal donor and the mesenter of a familial adenomatous polyposis (FAP) patient.	Fura-2	66-72	kininogen 1	Homo sapiens	0-10
8874220-5	1996	In addition, fluorescence measurements with Fura-2-labeled eosinophils in the presence of EGTA indicated Ca(2+)-mobilization from intracellular stores by Eotaxin.	Fura-2	44-50	C-C motif chemokine ligand 11	Homo sapiens	154-161
8913359-3	1996	By videomicroscopically examining Fura-2-loaded cells, we demonstrate that vasopressin induces a dose-dependent and receptor-mediated calcium influx fully inhibited by either 1 microM nifedipine or a pertussis toxin pretreatment and potentiated by 1 microM BAY K 8644.	Fura-2	34-40	arginine vasopressin	Rattus norvegicus	75-86
8917448-6	1996	In addition to the NO/cGMP-mediated Ca2+ influx into the fura-sensitive compartment, bradykinin enhanced 45Ca uptake into Ca stores that were not accessible to fura-2.	Fura-2	160-166	kininogen 1	Homo sapiens	85-95
8831516-2	1996	In the present study, we report the first endothelial [Ca2+]i quantification by the fura 2 method in microscopically imaged venular capillaries of the isolated blood-perfused rat lung.	Fura-2	84-90	carbonic anhydrase 2	Rattus norvegicus	55-58
8897698-7	1996	In Fura-2/AM loaded platelets, the rise of intracellular calcium level challenged by AA, collagen and thrombin were inhibited by C85.	Fura-2	3-9	coagulation factor II, thrombin	Homo sapiens	102-110
8939445-6	1996	TPEN, however, penetrates into synaptoneurosomes and efficiently displaces Cd2+ from the Fura-2-Cd2+ complex as shown by Fura-2 fluorescence recordings.	Fura-2	89-95	Cd2 molecule	Rattus norvegicus	75-78
8939445-6	1996	TPEN, however, penetrates into synaptoneurosomes and efficiently displaces Cd2+ from the Fura-2-Cd2+ complex as shown by Fura-2 fluorescence recordings.	Fura-2	89-95	Cd2 molecule	Rattus norvegicus	96-99
8795640-5	1996	Here, we report that NPY caused a long-term depression in both electrical activity and intracellular calcium levels of neurons, as studied with whole-cell patch-clamp recording and Fura-2 digital imaging.	Fura-2	181-187	neuropeptide Y	Homo sapiens	21-24
8831566-8	1996	These in vitro changes in fura-2 fluorescence, nevertheless, were small relative to changes in fura-2 fluorescence produced by elevation in intracellular Ca2+ concentrations in response to physiological stimulation of the cells (serum feeding after serum starvation).	Fura-2	95-101	carbonic anhydrase 2	Mus musculus	154-157
8914860-5	1996	The cytosolic Ca2+ mobilization in response to the NK1 agonist was monitored both by spectrofluorimetric and single-cell image analysis determinations on adherent cells loaded with the Ca(2+)-sensitive fluorescent indicators Fura-2/AM and Indo-1, respectively.	Fura-2	225-231	tachykinin receptor 1	Homo sapiens	51-54
8780286-2	1996	Using digital imaging microscopy with the Ca2+ indicator fura-2, the effects of halothane on the intracellular [Ca2+] dynamics induced by arginine vasopressin (AVP) in the perinuclear region and cytosol in single cultured smooth muscle cells of rat aorta were studied.	Fura-2	57-63	arginine vasopressin	Rattus norvegicus	147-158
8780286-2	1996	Using digital imaging microscopy with the Ca2+ indicator fura-2, the effects of halothane on the intracellular [Ca2+] dynamics induced by arginine vasopressin (AVP) in the perinuclear region and cytosol in single cultured smooth muscle cells of rat aorta were studied.	Fura-2	57-63	arginine vasopressin	Rattus norvegicus	160-163
8712453-4	1996	METHODS: The effect of halothane and isoflurane on the Ca2+ response to bradykinin of bovine aortic endothelial (BAE) cells was investigated using the fluorescent Ca2+ indicator fura-2.	Fura-2	178-184	kininogen 1	Bos taurus	72-82
8892530-2	1996	Because these drugs are frequently used in combination, the present study was designed to investigate how they interact to modify the Ca2+ transient and associated contraction in fura2-loaded cardiomyocytes.	Fura-2	179-184	carbonic anhydrase 2	Homo sapiens	134-137
8756565-3	1996	Video microscopy experiments performed on fura-2-loaded adrenal medullary or adrenal glomerulosa cell primary cultures showed that vasopressin dose dependently mobilized intracellular calcium, suggesting that functional vasopressin receptors are expressed in these tissues.	Fura-2	42-48	arginine vasopressin	Rattus norvegicus	131-142
8756565-3	1996	Video microscopy experiments performed on fura-2-loaded adrenal medullary or adrenal glomerulosa cell primary cultures showed that vasopressin dose dependently mobilized intracellular calcium, suggesting that functional vasopressin receptors are expressed in these tissues.	Fura-2	42-48	arginine vasopressin	Rattus norvegicus	220-231
8752105-2	1996	Bath perfusion or rapid puffer application of angiotensin II (Ang II) (1-100 nM) increased [Ca2+]i in both polygonal and stellate astroglia when measured using fura-2 imaging fluorescence microscopy.	Fura-2	160-166	angiotensinogen	Rattus norvegicus	46-60
8752105-2	1996	Bath perfusion or rapid puffer application of angiotensin II (Ang II) (1-100 nM) increased [Ca2+]i in both polygonal and stellate astroglia when measured using fura-2 imaging fluorescence microscopy.	Fura-2	160-166	angiotensinogen	Rattus norvegicus	62-68
8914862-3	1996	Functional coupling in hCCK-B.CHO cells was demonstrated using agonist stimulated mobilization of intracellular Ca2+, measured with the FURA-2 technique.	Fura-2	136-142	cholecystokinin B receptor	Homo sapiens	23-29
8760070-3	1996	PLB-deficient myocytes also demonstrated significant increases in the peak amplitude of the fura 2 fluorescence ratio and the rates of rising and falling phases of the Ca2+ transient.	Fura-2	92-98	phospholamban	Mus musculus	0-3
8753870-8	1996	Fura-2-based videoimaging showed that treatment with gp120 enhanced the ability of NMDA to increase neuronal [Ca2+]i.	Fura-2	0-6	inter-alpha-trypsin inhibitor heavy chain 4	Homo sapiens	53-58
8864566-1	1996	Digital-imaging microscopy of Fura-2-loaded Chinese hamster ovary cells, stably expressing the cholecystokinin-A receptor, revealed that both the C-terminal octapeptide of cholecystokinin (CCKB) and its analogue JMV-180, which acts as an agonist at the high-affinity CCK-A receptor, recruited CHO-CCK-A cells dose-dependently in terms of receptor-mediated Ca2+ mobilization.	Fura-2	30-36	cholecystokinin receptor type A	Cricetulus griseus	95-121
8805305-5	1996	RESULTS: We detected subcellular Ca2+ signals during the response of single Fura2-loaded HeLa cells to histamine.	Fura-2	76-81	carbonic anhydrase 2	Homo sapiens	33-36
8662890-7	1996	Of the chemokines tested, only murine MIP-1alpha, human and murine MIP-1beta and RANTES, human MCP-2, and JE were able to induce mobilization of intracellular Ca2+ from fura-2-loaded HEK 293 cells expressing the receptor.	Fura-2	169-175	chemokine (C-C motif) ligand 3	Mus musculus	38-48
8662890-7	1996	Of the chemokines tested, only murine MIP-1alpha, human and murine MIP-1beta and RANTES, human MCP-2, and JE were able to induce mobilization of intracellular Ca2+ from fura-2-loaded HEK 293 cells expressing the receptor.	Fura-2	169-175	chemokine (C-C motif) ligand 4	Mus musculus	67-76
8662890-7	1996	Of the chemokines tested, only murine MIP-1alpha, human and murine MIP-1beta and RANTES, human MCP-2, and JE were able to induce mobilization of intracellular Ca2+ from fura-2-loaded HEK 293 cells expressing the receptor.	Fura-2	169-175	chemokine (C-C motif) ligand 5	Mus musculus	81-87
8662890-7	1996	Of the chemokines tested, only murine MIP-1alpha, human and murine MIP-1beta and RANTES, human MCP-2, and JE were able to induce mobilization of intracellular Ca2+ from fura-2-loaded HEK 293 cells expressing the receptor.	Fura-2	169-175	C-C motif chemokine ligand 8	Homo sapiens	95-100
8782088-7	1996	When intracellular calcium mobilization was measured by Fura-2/AM cell labeling and spectrofluorometric monitoring, bombesin (100 nM) induced rapid calcium mobilization in both PC-3 (> 200% of baseline) and DU-145 (> 100% of baseline) cells, but not in LNCaP cells.	Fura-2	56-62	gastrin releasing peptide	Homo sapiens	116-124
8813647-1	1996	We have evaluated the application of the fura-2 method to detect cytosolic Ca2+ increase in gastric cells expressing CCKB/gastrin receptors, in order to screen gastrin receptor antagonists, as an alternative to functional studies.	Fura-2	41-47	gastrin/cholecystokinin type B receptor	Oryctolagus cuniculus	117-121
8813647-1	1996	We have evaluated the application of the fura-2 method to detect cytosolic Ca2+ increase in gastric cells expressing CCKB/gastrin receptors, in order to screen gastrin receptor antagonists, as an alternative to functional studies.	Fura-2	41-47	gastrin	Oryctolagus cuniculus	122-129
8762095-4	1996	Ca2+ release was measured by microfluorometry with Fura-2.	Fura-2	51-57	carbonic anhydrase 2	Oryctolagus cuniculus	0-3
8967450-1	1996	In freshly isolated individual rat adipocytes, cytosolic free Ca2+ concentration ([Ca2+]i) as measured with fura 2 slowly declined during incubation but was sustained, or even somewhat increased, by brief treatment with growth hormone (GH) at the beginning of a 3-h incubation period.	Fura-2	108-114	gonadotropin releasing hormone receptor	Rattus norvegicus	220-234
8627363-4	1996	Fura-2 Ca2+ imaging showed that an increase in presynaptic cytosolic Ca2+ ([Ca2+]i) accompanied the synaptic potentiation by BDNF, whereas no change in [Ca2+]i was observed during synaptic potentiation by CNTF.	Fura-2	0-6	LOC108713815	Xenopus laevis	125-129
8967451-0	1996	Growth hormone increases calcium uptake in rat fat cells by a mechanism dependent on protein kinase C. Growth hormone (GH; 500 ng/ml) rapidly doubled cytosolic free Ca2+ concentration ([Ca2+]i) in rat adipocytes as determined with the Ca2+ indicator fura 2.	Fura-2	250-256	gonadotropin releasing hormone receptor	Rattus norvegicus	0-14
8967451-0	1996	Growth hormone increases calcium uptake in rat fat cells by a mechanism dependent on protein kinase C. Growth hormone (GH; 500 ng/ml) rapidly doubled cytosolic free Ca2+ concentration ([Ca2+]i) in rat adipocytes as determined with the Ca2+ indicator fura 2.	Fura-2	250-256	gonadotropin releasing hormone receptor	Rattus norvegicus	103-117
8754275-0	1996	[Study of calcium-binding properties of the Mts-1 protein and its recombinant analog using the fluorescent probe Fura-2].	Fura-2	113-119	malignant transformation suppression-1	Homo sapiens	44-49
8754275-1	1996	A procedure based on the ability of Mts-1 to competitively inhibit the formation of Fura-2 complexes with Ca(2+)-ions is described.	Fura-2	84-90	malignant transformation suppression-1	Homo sapiens	36-41
8761891-5	1996	Fura-2 was used to measure basal and angiotensin II-stimulated intracellular free calcium concentration.	Fura-2	0-6	angiotensinogen	Rattus norvegicus	37-51
8741218-4	1996	Parvalbumin-transfected and mock-transfected cells were loaded with the calcium indicator fura-2 and were exposed, in the same dish, to different concentrations of the calcium ionophore A23187 or to KCI.	Fura-2	90-96	parvalbumin	Homo sapiens	0-11
8611703-1	1996	Changes in intracellular calcium concentration [Ca2+]i of fura-2-loaded human platelet during its adhesion to a fibrinogen-coated surface were studied, using a flow chamber mounted on an epifluorescence microscope equipped with digital-ratio imaging.	Fura-2	58-64	fibrinogen beta chain	Homo sapiens	112-122
8641274-3	1996	When Fura 2 was used to report the luminal free [Ca2+] after inhibition of further Ca2+ uptake, submaximal concentrations of InsP3 caused rapid, partial decreases in fluorescence ratios.	Fura-2	5-11	carbonic anhydrase 2	Homo sapiens	49-52
8641274-3	1996	When Fura 2 was used to report the luminal free [Ca2+] after inhibition of further Ca2+ uptake, submaximal concentrations of InsP3 caused rapid, partial decreases in fluorescence ratios.	Fura-2	5-11	carbonic anhydrase 2	Homo sapiens	83-86
8641274-7	1996	In stores co-loaded with both indicators, InsP3-evoked Ca2+ release appeared quantal with Fura 2 and largely non-quantal with Calcium Green-5N; the discrepancy is not, therefore, a direct effect of the indicators.	Fura-2	90-96	carbonic anhydrase 2	Homo sapiens	55-58
8625903-1	1996	As the GnRH-induced secretion of gonadotropins is critically dependent upon an increase in the intracellular calcium ion concentration ([Ca2+]i) and modulated by gonadal factors, the effects of gonadal steroids on the pattern of calcium mobilization in single gonadotrophs of the male rat were examined using the fluorescent Ca2+ indicator fura-2/AM.	Fura-2	340-346	gonadotropin releasing hormone 1	Rattus norvegicus	7-11
8626395-1	1996	Regulation of the cytosolic free Ca2+ concentration by nerve growth factor was investigated in C6-2B glioma cells newly expressing the high affinity nerve growth factor receptor trkA, using Fura-2 fluorescence ratio imaging.	Fura-2	190-196	nerve growth factor	Mus musculus	55-74
8848347-5	1996	Fura-2-loaded washed platelets were stimulated with the agonists collagen (2 micrograms/mL) or thrombin (1.0 U/mL).	Fura-2	0-6	coagulation factor II, thrombin	Homo sapiens	95-103
8683457-5	1996	Application of 20 mM caffeine released Ca2+ from the sarcoplasmic reticulum (SR), resulting in a transient increase in the fura-2 fluorescence ratio.	Fura-2	123-129	carbonic anhydrase 2	Rattus norvegicus	39-42
8603609-2	1996	We measured [Ca2+]i with fura-2 and recorded ion currents and membrane potentials with the whole cell configuration of the patch-clamp technique to elucidate the mechanism of PACAP-induced [Ca2+]i increase in bovine adrenal chromaffin cells.	Fura-2	25-31	adenylate cyclase activating polypeptide 1	Bos taurus	175-180
8984102-1	1996	To clarify the relationship between cellular Ca2+ handling and salt sensitivity, we evaluated cytosolic free Ca2+ ([Ca2+]i) in fura-2 loaded platelets isolated from 20 inpatients with essential hypertension.	Fura-2	127-133	carbonic anhydrase 2	Homo sapiens	109-112
8984102-1	1996	To clarify the relationship between cellular Ca2+ handling and salt sensitivity, we evaluated cytosolic free Ca2+ ([Ca2+]i) in fura-2 loaded platelets isolated from 20 inpatients with essential hypertension.	Fura-2	127-133	carbonic anhydrase 2	Homo sapiens	109-112
8789126-1	1996	The effect of the fluorescent Ca2+ indicator dye Fura-2 on Ca2+ dynamics was studied in proximal apical dendrites of neocortical layer V and hippocampal CA1 pyramidal neurons in rat brain slices using somatic whole-cell recording and a charge-coupled device camera.	Fura-2	49-55	carbonic anhydrase 2	Rattus norvegicus	59-62
8789126-1	1996	The effect of the fluorescent Ca2+ indicator dye Fura-2 on Ca2+ dynamics was studied in proximal apical dendrites of neocortical layer V and hippocampal CA1 pyramidal neurons in rat brain slices using somatic whole-cell recording and a charge-coupled device camera.	Fura-2	49-55	carbonic anhydrase 1	Rattus norvegicus	153-156
8789126-2	1996	A single action potential evoked a transient increase of intradendritic calcium concentration ([Ca2+]i) that was reduced in size and prolonged when the Fura-2 concentration was increased from 20 to 250 microM.	Fura-2	152-158	carbonic anhydrase 2	Rattus norvegicus	96-99
8815209-3	1996	For this purpose, the Ca2+ fraction of the total cation current (named "fractional Ca2+ current") was measured directly from the change in the Ca(2+)-sensitive fura-2 fluorescence at 380 nm excitation wavelength.	Fura-2	160-166	carbonic anhydrase 2	Rattus norvegicus	22-25
8815209-3	1996	For this purpose, the Ca2+ fraction of the total cation current (named "fractional Ca2+ current") was measured directly from the change in the Ca(2+)-sensitive fura-2 fluorescence at 380 nm excitation wavelength.	Fura-2	160-166	carbonic anhydrase 2	Rattus norvegicus	83-86
8593933-3	1996	[Ca2+]i in single rat pancreatic beta-cells was measured by dual-wavelength fura-2 microfluorometry.	Fura-2	76-82	carbonic anhydrase 2	Rattus norvegicus	1-4
8611034-2	1996	In fura-2-loaded cells, AVP induced a rapid (0.5-2 min) transient increase in [Ca2+]i that was followed by a smaller sustained increase in [Ca2+]i.	Fura-2	3-9	arginine vasopressin	Rattus norvegicus	24-27
9075574-5	1996	Ristocetin-mediated binding of vWF to human platelets promoted a slow rise in Fura-2 fluorescence ratio.	Fura-2	78-84	von Willebrand factor	Homo sapiens	31-34
8821822-4	1996	Fura-2 fluorescence was measured in an inverted microscope at 37 degrees C. Angiotensin II (0.1 nmol/liter) and ATP (0.1 mumol/liter) induced a rapid transient increase of [Ca2+]i, which was followed by a sustained plateau (N = 37 and N = 24).	Fura-2	0-6	angiotensinogen	Rattus norvegicus	76-90
8717425-4	1996	Results from Fura-2 microfluorometry and Fluo-3 confocal microscopy suggest a potential cross talk between the increase of cytosolic free Ca2+ and the uptake and release of Ca2+ by the nucleus during spontaneous contraction of single myocytes.	Fura-2	13-19	carbonic anhydrase 2	Gallus gallus	138-141
8717425-4	1996	Results from Fura-2 microfluorometry and Fluo-3 confocal microscopy suggest a potential cross talk between the increase of cytosolic free Ca2+ and the uptake and release of Ca2+ by the nucleus during spontaneous contraction of single myocytes.	Fura-2	13-19	carbonic anhydrase 2	Gallus gallus	173-176
8573071-9	1996	Using fura 2-loaded platelets, the rise in the cytosolic free Ca2+ concentration induced by cathepsin G was shown to result, as for thrombin, from both mobilization of internal stores and Ca2+ entry across the plasma membrane.	Fura-2	6-12	cathepsin G	Homo sapiens	92-103
12013483-12	1996	To determine whether the depression of vascular contraction by these agents was correlated with cell calcium changes, the angiotensin II-induced calcium mobilization was determined in Fura-2 loaded cells, before and after treatment with these inhibitors.	Fura-2	184-190	angiotensinogen	Rattus norvegicus	122-136
8721612-4	1996	Further detection of a rise in the intracellular free Ca2+ concentration ([Ca2+]i) in cells exposed to THQ plus Ca2+, together with the partial protection conferred by the intracellular Ca(2+)-chelator fura-2 against cell growth inhibition, indicated that a disruption of Ca2+ homeostasis is a determinant event in THQ cytotoxicity.	Fura-2	202-208	carbonic anhydrase 2	Cricetulus griseus	54-57
8721612-4	1996	Further detection of a rise in the intracellular free Ca2+ concentration ([Ca2+]i) in cells exposed to THQ plus Ca2+, together with the partial protection conferred by the intracellular Ca(2+)-chelator fura-2 against cell growth inhibition, indicated that a disruption of Ca2+ homeostasis is a determinant event in THQ cytotoxicity.	Fura-2	202-208	carbonic anhydrase 2	Cricetulus griseus	75-78
8721612-4	1996	Further detection of a rise in the intracellular free Ca2+ concentration ([Ca2+]i) in cells exposed to THQ plus Ca2+, together with the partial protection conferred by the intracellular Ca(2+)-chelator fura-2 against cell growth inhibition, indicated that a disruption of Ca2+ homeostasis is a determinant event in THQ cytotoxicity.	Fura-2	202-208	carbonic anhydrase 2	Cricetulus griseus	75-78
8721612-4	1996	Further detection of a rise in the intracellular free Ca2+ concentration ([Ca2+]i) in cells exposed to THQ plus Ca2+, together with the partial protection conferred by the intracellular Ca(2+)-chelator fura-2 against cell growth inhibition, indicated that a disruption of Ca2+ homeostasis is a determinant event in THQ cytotoxicity.	Fura-2	202-208	carbonic anhydrase 2	Cricetulus griseus	75-78
8871886-2	1996	AVP (ED50, 10(-7) mol/l) and oxytocin (ED50, 3 x 10(-8) mol/l) induced a rapid, transient and dose-dependent increase in [Ca2+]i as detected by fura-2 microfluorimetry.	Fura-2	144-150	arginine vasopressin	Homo sapiens	0-3
7488000-2	1995	Fura-2 fluorometry revealed that ADM decreases both the cytosolic Ca2+ concentration ([Ca2+]i) and tension induced by 0.3 microM phenylephrine (PE) in porcine RASMCs.	Fura-2	0-6	adrenomedullin	Homo sapiens	33-36
8961380-4	1996	Substance P (30 microM) also induced a transient increase in the intracellular Ca2+ concentration ([Ca2+]i) in both synovial A and B cells as measured by a Ca2+ indicator, fura 2, BAPTA-AM and an inositol-1,4-5-triphosphate (IP3) receptor antagonist, heparin, inhibited the substance P induced increase in [Ca2+]i, but they had no effects on oxyradical production.	Fura-2	172-178	tachykinin precursor 1	Homo sapiens	0-11
8822433-5	1995	The SABP also induces an increase in intracellular Ca2+ in spermatozoa, as seen by FURA-2 AM studies.	Fura-2	83-89	prolactin induced protein	Homo sapiens	4-8
8848007-4	1995	Pharmacological and functional analyses carried out on Fura-2-loaded cells showed that Ca2+ influx elicited by both growth factors is the summation of two distinct pathways, with the major pathway dependent on and the minor pathway independent of store depletion.	Fura-2	55-61	carbonic anhydrase 2	Homo sapiens	87-90
9275708-1	1996	Using fluorescent probe Fura-2/AM, ginsenoside Rb1 (10, 50 and 100 mumol.L-1) was shown to dose-dependently reduce intracellular Ca2+ concentration of freshly dissociated brain cells isolated from newborn rats.	Fura-2	24-30	RB transcriptional corepressor 1	Rattus norvegicus	47-50
8934635-4	1995	Ricin pretreatment nearly doubled the intracellular-free Ca2+ concentration as measured by fura-2 fluorescence microscopy in isolated myocytes (p = 0.002).	Fura-2	91-97	ricin	Ricinus communis	0-5
8719876-5	1995	With single cell measurements of fura-2 loaded cells we demonstrate that SP-A raises the intracellular free calcium ion concentration 6 to 8 seconds after addition.	Fura-2	33-39	surfactant protein A1	Rattus norvegicus	73-77
7554143-7	1995	Furthermore, the thrombin-induced rise in cytoplasmic [Ca2+]i was strongly attenuated by the cGMP-PK activator in fura 2-loaded aorta ECs.	Fura-2	114-120	coagulation factor II, thrombin	Homo sapiens	17-25
7568127-7	1995	The [Ca2+]i response to glutamate (quantified by fura-2 imaging) was markedly potentiated in neurons exposed to A beta, and this action of A beta was suppressed in cultures pretreated with TNFs.	Fura-2	49-55	amyloid beta precursor protein	Homo sapiens	112-118
8589290-6	1995	Fura-2 fluorescence ratio was not significantly changed with angiotensin II, Ap3A, or Ap4A but increased with vasopressin, ATP, Ap5A, and Ap6A by 0.08 +/- 0.02 (N = 13), 0.04 +/- 0.02 (N = 13), 0.03 +/- 0.01 (N = 14), and 0.03 +/- 0.01 (N = 10), respectively.	Fura-2	0-6	arginine vasopressin	Rattus norvegicus	110-121
7485450-5	1995	In fura 2-loaded proximal tubule cells, EGF caused a concentration-dependent increase in cytosolic Ca2+ concentration ([Ca2+]i), due to Ca2+ influx, which was inhibited by either ketoconazole or SKF-525A but not by indomethacin.	Fura-2	3-9	pro-epidermal growth factor	Oryctolagus cuniculus	40-43
7568127-7	1995	The [Ca2+]i response to glutamate (quantified by fura-2 imaging) was markedly potentiated in neurons exposed to A beta, and this action of A beta was suppressed in cultures pretreated with TNFs.	Fura-2	49-55	amyloid beta precursor protein	Homo sapiens	139-145
8519599-3	1995	Exposure of myo-[2-3H]-inositol prelabelled fibroblasts to HDL3 or LDL elicited major increases in IP1 and minor increases in IP2 and IP3 within 30 s. In fura-2 loaded suspended fibroblasts, HDL3 and LDL increased intracellular Ca2+ concentrations ([Ca2+]i) with comparable rapid, transient kinetics.	Fura-2	154-160	HDL3	Homo sapiens	59-63
8519599-3	1995	Exposure of myo-[2-3H]-inositol prelabelled fibroblasts to HDL3 or LDL elicited major increases in IP1 and minor increases in IP2 and IP3 within 30 s. In fura-2 loaded suspended fibroblasts, HDL3 and LDL increased intracellular Ca2+ concentrations ([Ca2+]i) with comparable rapid, transient kinetics.	Fura-2	154-160	HDL3	Homo sapiens	191-195
7550072-1	1995	In fura-2-loaded human periodontal ligament (HPDL) cells, bradykinin induced a rapidly transient increase and subsequently sustained increase in cytosolic Ca2+ ([Ca2+]i).	Fura-2	3-9	kininogen 1	Homo sapiens	58-68
9816080-5	1995	In fura-2-loaded ovarian carcinoma cells, ET-1 induced dose-dependent increases in cytoplasmic Ca2+ concentration.	Fura-2	3-9	endothelin 1	Homo sapiens	42-46
7501705-10	1995	(4) Using FURA-2 to monitor intracellular free calcium simultaneous with contractile tension, we demonstrated that the alterations in the contractile responses observed at different concentrations of extracellular Mg2+ correlated with similar changes in intracellular free calcium.	Fura-2	10-16	mucin 7, secreted	Homo sapiens	214-217
7582546-2	1995	The contribution of sarco-endoplasmic reticulum Ca(2+)-ATPases (SERCA)-regulated Ca2+ stores to the increase in intracellular free calcium ([Ca2+]i) induced by bradykinin (BK) was investigated in fura-2 loaded human tracheal smooth muscle cells (TSMC).	Fura-2	196-202	kininogen 1	Homo sapiens	160-170
7478924-9	1995	A half-maximal initial increase in the fura-2 fluorescence ratio was reached at 22 nmol/l, 0.9 micromol/l, 2.0 micromol/l and 4.0 micromol/l with Ang II, Ap3A, ATP and Ap4A, respectively.	Fura-2	39-45	angiotensinogen	Rattus norvegicus	146-152
8564193-2	1995	In the present study, we determined whether ETA receptors are present on endothelial cells in situ, by use of front-surface fluorometry of fura-2-loaded porcine aortic valvular strips and reverse transcription polymerase chain reaction (RT-PCR).	Fura-2	139-145	endothelin receptor type A	Sus scrofa	44-47
8537818-6	1995	The value of beta, the buffering power of myoplasm, was determined in fibers equilibrated with a combination of EGTA, phenol red, and fura-2; its mean value was 22 mM/pH unit.	Fura-2	134-140	potassium calcium-activated channel subfamily M regulatory beta subunit 2	Homo sapiens	13-17
7585889-1	1995	The effect of an induction of transforming Ha-ras on Ca2+ influx into NIH3T3 cells was studied employing Fura-2 quenching by Mn2+.	Fura-2	105-111	Harvey rat sarcoma virus oncogene	Mus musculus	43-49
7585894-2	1995	ATP induced an increase in [Ca2+]i of these epithelial cells loaded with Fura-2 in a dose-dependent manner (1-100 microM).	Fura-2	73-79	carbonic anhydrase 2	Homo sapiens	28-31
7632163-10	1995	The two fenamates inhibited the fMLP-induced increase in intracellular free calcium in fura-2 loaded PMNs in the presence but not in the absence of extracellular calcium.	Fura-2	87-93	formyl peptide receptor 1	Homo sapiens	32-36
7631736-2	1995	Digitized image analysis of fura 2-loaded human embryonic lung fibroblasts indicated specific transient increases in [Ca2+]i beginning in some cells within the first 5 min of incubation with cross-linked MAb2 (70-750 nM, P < 0.01), which were similar in timing and intracellular distribution to those induced by HCMV.	Fura-2	28-34	carbonic anhydrase 2	Homo sapiens	118-121
7750471-5	1995	In fura 2-loaded VSMC, 5-min incubation with PTH or PTHrP attenuated angiotensin II (Ang II)-induced calcium mobilization, an effect that was reduced by preincubation of VSMC with PTH for 1.5 h. Similarly, 1.5-h preincubation with PTH or PTHrP decreased the cAMP response to these peptides but not to forskolin or NaF.	Fura-2	3-9	parathyroid hormone	Bos taurus	45-48
7635245-3	1995	In human platelets loaded with fura-2, the intracellular rise of [Ca2+] induced by thrombin was dose-dependently inhibited by MM-706 with an approximate IC50 of 100 microM.	Fura-2	31-37	coagulation factor II, thrombin	Homo sapiens	83-91
7603033-6	1995	The change in [Ca2+]i in thymocytes after TPEN treatment was monitored using the fluorescence Ca2+ indicator dye, fura-2.	Fura-2	114-120	carbonic anhydrase 2	Homo sapiens	15-18
7539422-6	1995	Calcium-permeable cation channel activity was detected in CD20-expressing cells by whole cell patch clamp recording and microfluorometric determination of the cytoplasmic Ca2+ concentration using fura-2.	Fura-2	196-202	membrane-spanning 4-domains, subfamily A, member 1	Mus musculus	58-62
7750471-5	1995	In fura 2-loaded VSMC, 5-min incubation with PTH or PTHrP attenuated angiotensin II (Ang II)-induced calcium mobilization, an effect that was reduced by preincubation of VSMC with PTH for 1.5 h. Similarly, 1.5-h preincubation with PTH or PTHrP decreased the cAMP response to these peptides but not to forskolin or NaF.	Fura-2	3-9	parathyroid hormone like hormone	Bos taurus	52-57
7750471-5	1995	In fura 2-loaded VSMC, 5-min incubation with PTH or PTHrP attenuated angiotensin II (Ang II)-induced calcium mobilization, an effect that was reduced by preincubation of VSMC with PTH for 1.5 h. Similarly, 1.5-h preincubation with PTH or PTHrP decreased the cAMP response to these peptides but not to forskolin or NaF.	Fura-2	3-9	parathyroid hormone	Bos taurus	52-55
7750471-5	1995	In fura 2-loaded VSMC, 5-min incubation with PTH or PTHrP attenuated angiotensin II (Ang II)-induced calcium mobilization, an effect that was reduced by preincubation of VSMC with PTH for 1.5 h. Similarly, 1.5-h preincubation with PTH or PTHrP decreased the cAMP response to these peptides but not to forskolin or NaF.	Fura-2	3-9	parathyroid hormone	Bos taurus	52-55
7744827-3	1995	Occupation of either receptor species, with 100 nM peptide YY (PYY) or 10 microM phenylephrine (PE), respectively, resulted in a rapid increase in the cytoplasmic free calcium concentration ([Ca2+]i) as assessed with Fura-2/AM.	Fura-2	217-223	peptide YY	Cricetulus griseus	51-61
7666368-2	1995	Arg8-vasopressin (AVP)-regulated Ca2+ transport were investigated in fura-2-loaded A7r5 cells using both single cell and population measurements.	Fura-2	69-75	arginine vasopressin	Rattus norvegicus	0-16
7666368-2	1995	Arg8-vasopressin (AVP)-regulated Ca2+ transport were investigated in fura-2-loaded A7r5 cells using both single cell and population measurements.	Fura-2	69-75	arginine vasopressin	Rattus norvegicus	18-21
7539257-2	1995	In the present study we show that in Fura 2 loaded GH4C1 cells, caffeine inhibited, in a dose-dependent manner, the Ca2+ response induced by a submaximally effective dose (3 nM) of thyrotropin-releasing hormone (TRH).	Fura-2	37-43	thyrotropin releasing hormone	Rattus norvegicus	181-210
7744827-3	1995	Occupation of either receptor species, with 100 nM peptide YY (PYY) or 10 microM phenylephrine (PE), respectively, resulted in a rapid increase in the cytoplasmic free calcium concentration ([Ca2+]i) as assessed with Fura-2/AM.	Fura-2	217-223	peptide YY	Cricetulus griseus	63-66
7729011-4	1995	In cultured bovine aortic endothelial cells, extracellular application of bradykinin (BK, 10 to 20 mumol/L) caused transient (sometimes oscillatory) increase in [Ca2+]i, which was measured with the fluorescent Ca2+ indicator fura 2 and fluorescence imaging microscopy.	Fura-2	225-231	kininogen 1	Bos taurus	74-84
7729011-4	1995	In cultured bovine aortic endothelial cells, extracellular application of bradykinin (BK, 10 to 20 mumol/L) caused transient (sometimes oscillatory) increase in [Ca2+]i, which was measured with the fluorescent Ca2+ indicator fura 2 and fluorescence imaging microscopy.	Fura-2	225-231	kininogen 1	Bos taurus	86-88
7758834-3	1995	In the present study, the AVP-induced changes in cytosolic free Ca2+ ([Ca2+]i) were investigated in single fura-2 loaded insulin-secreting HIT cells.	Fura-2	107-113	insulin	Homo sapiens	121-128
7623276-13	1995	Measurements of free intracellular calcium concentration ([Ca2+]i) with the dye fura-2 showed that bFGF elicited sustained increases in [Ca2+]i that were completely dependent on external calcium and on the presence of the agonist and could last more than 1 h. 5.	Fura-2	80-86	fibroblast growth factor 2	Mus musculus	99-103
7606345-10	1995	Both the contractions induced directly by caffeine and those produced following caffeine wash-out after ryanodine treatment were accompanied by a maintained increase in intracellular Ca2+ concentration measured with fura-2.	Fura-2	216-222	carbonic anhydrase 2	Oryctolagus cuniculus	183-186
7895328-6	1995	In fura 2-loaded endothelial cells, inhibition of tyrosine kinases attenuated Ca2+ signaling after stimulation with either bradykinin (30 nmol/L) or thapsigargin (30 nmol/L).	Fura-2	3-9	kininogen 1	Homo sapiens	123-133
7535010-3	1995	With the use of the perforated patch technique and fura 2 microfluorimetry, a fivefold greater increase in [Ca2+]i per unit decrease in membrane potential was obtained in the presence of SP (10 nM) compared with resting state, implying that SP increased Ca2+ conductance.	Fura-2	51-57	tachykinin precursor 1	Homo sapiens	187-189
7772573-3	1995	In this study, BK-induced Ca2+ responses were examined in primary cultured human keratinocytes by video imaging fluorescence microscopy using fura-2.	Fura-2	142-148	kininogen 1	Homo sapiens	15-17
7700249-1	1995	Measurement of the intracellular Ca2+ concentration ([Ca2+]i) in fura-2-loaded single cells of the human neuroblastoma line SH-SY5Y indicated coexpression of muscarinic and bradykinin receptors linked to activation of phosphoinositidase C (PIC).	Fura-2	65-71	kininogen 1	Homo sapiens	173-183
7700249-1	1995	Measurement of the intracellular Ca2+ concentration ([Ca2+]i) in fura-2-loaded single cells of the human neuroblastoma line SH-SY5Y indicated coexpression of muscarinic and bradykinin receptors linked to activation of phosphoinositidase C (PIC).	Fura-2	65-71	phospholipase C beta 1	Homo sapiens	218-238
7700249-1	1995	Measurement of the intracellular Ca2+ concentration ([Ca2+]i) in fura-2-loaded single cells of the human neuroblastoma line SH-SY5Y indicated coexpression of muscarinic and bradykinin receptors linked to activation of phosphoinositidase C (PIC).	Fura-2	65-71	phospholipase C beta 1	Homo sapiens	240-243
7626323-2	1995	EGF significantly increased [Ca++]i measured in fura-2-loaded, individual cells.	Fura-2	48-54	epidermal growth factor like 1	Rattus norvegicus	0-3
7626327-2	1995	Cells loaded with the Ca2+ dye FURA-2 dose-dependently responded to CGRP (1-100 nM) with transient two-fold increases in [Ca2+]i.	Fura-2	31-37	calcitonin-related polypeptide alpha	Rattus norvegicus	68-72
7603843-1	1995	Ca2+ channel activities were recorded in the limb bud of embryonic day 4 chick with Ca2+ sensitive fluorescence (Fura-2) measurements and patch clamp techniques.	Fura-2	113-119	carbonic anhydrase 2	Gallus gallus	0-3
7876236-4	1995	We have studied possible intracellular signaling effects of the FVIIa-TF interaction by measuring cytosolic free Ca2+ in single fura-2-loaded cells and found that 200 nM FVIIa caused Ca2+ transients in about 30% of human umbilical vein endothelial cells treated with interleukin-1 beta to express TF, compared to below 5% in uninduced cells.	Fura-2	128-134	coagulation factor III, tissue factor	Homo sapiens	70-72
7756530-4	1995	k21 represents the second-order rate constant of binding of Ca2+ and Fura-2 in the excited state, whereas k12 is the first-order rate constant of dissociation of the excited Ca2+:Fura-2 complex.	Fura-2	179-185	keratin 12	Homo sapiens	106-109
7861766-6	1995	CCK octapeptide caused a specific increase in [Ca2+]i measured by Fura-2 fluorometry in H-PBMC and human T helper lymphocytes.	Fura-2	66-72	cholecystokinin	Homo sapiens	0-3
7859285-4	1995	Dextrans labeled with fluorescent Ca2+ indicators (calcium-Green 1 and Fura 2) are distributed uniformly in the nucleoplasm and can be used to show that changes in the external Ca2+ concentration produce rapid changes in the nucleoplasmic Ca2+ concentration.	Fura-2	71-77	carbonic anhydrase 2	Homo sapiens	34-37
7859285-4	1995	Dextrans labeled with fluorescent Ca2+ indicators (calcium-Green 1 and Fura 2) are distributed uniformly in the nucleoplasm and can be used to show that changes in the external Ca2+ concentration produce rapid changes in the nucleoplasmic Ca2+ concentration.	Fura-2	71-77	carbonic anhydrase 2	Homo sapiens	177-180
7859285-4	1995	Dextrans labeled with fluorescent Ca2+ indicators (calcium-Green 1 and Fura 2) are distributed uniformly in the nucleoplasm and can be used to show that changes in the external Ca2+ concentration produce rapid changes in the nucleoplasmic Ca2+ concentration.	Fura-2	71-77	carbonic anhydrase 2	Homo sapiens	177-180
7867789-1	1995	The potency of annexin V to transport Ca2+ ions across phospholipid membranes was investigated, using large unilamellar phospholipid vesicles loaded with the Ca2+ indicator fura-2.	Fura-2	173-179	annexin A5	Homo sapiens	15-24
7867789-3	1995	However, if the vesicles were first incubated with annexin V in the absence of Ca2+, subsequent addition of Ca2+ produced a fluorescence signal due to binding of Ca2+ to fura-2.	Fura-2	170-176	annexin A5	Homo sapiens	51-60
7867789-5	1995	Our results show that annexin V causes loss of vesicle integrity in the absence of Ca2+, and leakage of trapped fura-2, rather than inward Ca2+ transport.	Fura-2	112-118	annexin A5	Homo sapiens	22-31
7740516-1	1995	Treatment of Fura-2 loaded HEL cells, a human megakaryocyte-like cell line, with P2-purinoceptor nucleotide ligands (ADP, ATP, UTP, 2-methylthio-ATP) evoked a rise in cytosolic calcium.	Fura-2	13-19	pyrimidinergic receptor P2Y6	Homo sapiens	81-96
7550580-4	1995	PTH also increases intracellular calcium as measured by the technique using Fura-2 fluorescent dye and stimulates phosphoinositide degradation as evaluated by column chromatography.	Fura-2	76-82	parathyroid hormone	Homo sapiens	0-3
7536247-4	1995	Several P450 inhibitors diminished the sustained [Ca2+]i plateau response to agonist or intracellular Ca2+ store depletion with ATPase inhibitors by 31-69% (fura-2 technique).	Fura-2	157-163	dynein axonemal heavy chain 8	Homo sapiens	128-134
7530414-4	1995	The increase in intracellular calcium [Ca2+]in evoked by substance P was measured by the fluorescent indicator fura-2 loaded in a murine cell line expressing substance P (NK1) receptors.	Fura-2	111-117	tachykinin 1	Mus musculus	57-68
7530414-4	1995	The increase in intracellular calcium [Ca2+]in evoked by substance P was measured by the fluorescent indicator fura-2 loaded in a murine cell line expressing substance P (NK1) receptors.	Fura-2	111-117	tachykinin 1	Mus musculus	158-169
7623611-1	1995	Total gangliosides from bovine brain at micromolar concentration induce intracellular Ca2+ increments in a temperature, time and dose dependent manner when assayed with suspensions of rat macrophages, rat and chicken neurons, human erythrocytes and liposomes, loaded with the fluorescent Ca2+ indicator FURA 2.	Fura-2	303-309	carbonic anhydrase 2	Bos taurus	86-89
8001384-5	1995	MEASUREMENTS AND MAIN RESULTS: Endothelin-1 increased the intracellular calcium concentration in fura-2 loaded human monocytes to a mean value of 37 +/- 4 nmol.	Fura-2	97-103	endothelin 1	Homo sapiens	31-43
7529236-3	1995	We report that addition of vasopressin to fura-2-loaded glomerular mesangial cells in culture markedly accelerated the rate at which Mn2+ quenched fura-2 fluorescence at its Ca(2+)-insensitive wavelength in the presence of extracellular NaCl, but that this quench response was attenuated when Cl- was removed from the extracellular medium by equimolar substitution with impermeant anions (gluconate, methanesulfonate, acetate, lactate).	Fura-2	42-48	arginine vasopressin	Homo sapiens	27-38
7529236-3	1995	We report that addition of vasopressin to fura-2-loaded glomerular mesangial cells in culture markedly accelerated the rate at which Mn2+ quenched fura-2 fluorescence at its Ca(2+)-insensitive wavelength in the presence of extracellular NaCl, but that this quench response was attenuated when Cl- was removed from the extracellular medium by equimolar substitution with impermeant anions (gluconate, methanesulfonate, acetate, lactate).	Fura-2	147-153	arginine vasopressin	Homo sapiens	27-38
7529236-5	1995	Addition of the Cl- channel inhibitor, 5-nitro-2-(3-phenylpropylaminobenzoic acid) (NPPB), also inhibited Mn(2+)-induced quench of fura-2 fluorescence following vasopressin addition.	Fura-2	131-137	arginine vasopressin	Homo sapiens	161-172
7730791-4	1995	In dark-adapted photoreceptors, the initial Cai was 0.40 +/- 0.22 microM (SD, n = 7) as measured with fura-2.	Fura-2	102-108	carbonic anhydrase 1	Homo sapiens	44-47
7705470-1	1994	We examined the mechanism of endothelin-1-induced contraction in the porcine coronary artery by assessing the changes in myosin light chain phosphorylation and cytosolic Ca2+ concentration ([Ca2+]i) determined by fura-2 and front-surface fluorometry.	Fura-2	213-219	endothelin 1	Homo sapiens	29-41
7565458-4	1995	Using video image analysis of single cells loaded with fura-2, PTH (10-(7) M) induced transient increases in [Ca2+]i preferentially in cells in S phase, with 82% response frequency whereas cells in G1 phase responded poorly to PTH with only 10% response frequency.	Fura-2	55-61	parathyroid hormone	Rattus norvegicus	63-66
7479311-8	1995	In intact cells, angiotensin II caused a rapid increase in intracellular calcium (Ca2+) using Fura-2 as a Ca2+ indicator.	Fura-2	94-100	angiotensinogen	Rattus norvegicus	17-31
7962102-10	1994	Using fura-2 imaging of single cells, we show that soluble N-cadherin induces Ca2+ increases in CG neuron cell bodies, and, importantly, in growth cones.	Fura-2	6-12	cadherin 2	Gallus gallus	59-69
7964729-1	1994	Fura-2 digital imaging microfluorimetry was used to evaluate the Ca2+ signals generated in single clonal human neuroepithelioma cells (SK-N-MCIXC) in response to agonists that stimulate phosphoinositide hydrolysis.	Fura-2	0-6	hedgehog acyltransferase	Homo sapiens	135-139
7898400-7	1994	Measurements of intracellular calcium activity ([Ca2+]i) with the indicator dye fura-2 revealed a sharp increase during glutamate exposure.	Fura-2	80-86	carbonic anhydrase 2	Rattus norvegicus	49-52
7996447-2	1994	In the endothelium loaded with fura-2, ET-1 (1-100 nM) induced large transient increase followed by small sustained increase in cytosolic Ca++ level ([Ca++]i) in a concentration-dependent manner.	Fura-2	31-37	endothelin-1	Oryctolagus cuniculus	39-43
7996199-2	1994	Using fura-2-based imaging we found that Ca2+ transients evoked by depolarization with 50 mM KCl were suppressed by the mu-opioid receptor agonist D-Ala2,N-MePhe4,Gly5-ol-enkephalin (DAMGO) and less effectively by the kappa-receptor agonist U-69,593.	Fura-2	6-12	proenkephalin	Rattus norvegicus	171-181
7767879-2	1994	ATPase activity was measured using a phosphoenolpyruvate-pyruvate kinase regenerating system for ATP that was monitored by NADH fluorescence changes, and Ca2+ was measured using fura 2 fluorescence.	Fura-2	178-184	dynein axonemal heavy chain 8	Homo sapiens	0-6
7523388-4	1994	We also analyzed the kinetics of activation of intracellular IP3 receptor/Ca2+ channels by monitoring the quench of sequestered dye by the entry of cytoplasmic Mn2+ into fura-2-loaded intracellular IP3-sensitive organelles.	Fura-2	170-176	inositol 1,4,5-trisphosphate receptor, type 3	Rattus norvegicus	61-73
8083757-2	1994	After selectively loading presynaptic structures in area CA1 with the calcium indicator fura-2, we simultaneously recorded a presynaptic calcium transient ([Ca]t) and the corresponding field excitatory postsynaptic potential (fEPSP) evoked by a single stimulus given to the Schaffer collateral-commissural (SCC) pathway.	Fura-2	88-94	carbonic anhydrase 1	Homo sapiens	57-60
7530208-1	1994	The nature of the senktide response of the human NK3 receptor expressed in Chinese hamster ovary cells was characterised using the Ca2+ sensitive dye Fura-2 and imaging methods.	Fura-2	150-156	tachykinin receptor 3	Homo sapiens	49-61
7523185-2	1994	Fura-2 measurements revealed that the RYR in T-cells functions as a ryanodine-sensitive, caffeine-insensitive Ca2+ release channel.	Fura-2	0-6	ryanodine receptor 1	Homo sapiens	38-41
7520361-4	1994	Indeed, in fura-2-loaded cells, PAF stimulates [Ca2+]i increase with a median effective concentration of 5.6 nM.	Fura-2	11-17	PCNA clamp associated factor	Homo sapiens	32-35
7914703-1	1994	Fura-2 and imaging technology were used to detect intracellular Ca2+ changes in CA1 pyramidal cells in hippocampal slices.	Fura-2	0-6	carbonic anhydrase 2	Homo sapiens	64-67
7830331-1	1994	We measured [Ca2+]i of guinea pig and rat myocytes with Ca2+ waves, using fura-2 fluorescence image processing.	Fura-2	74-80	LOW QUALITY PROTEIN: carbonic anhydrase 2	Cavia porcellus	13-16
7914703-1	1994	Fura-2 and imaging technology were used to detect intracellular Ca2+ changes in CA1 pyramidal cells in hippocampal slices.	Fura-2	0-6	carbonic anhydrase 1	Homo sapiens	80-83
7916897-8	1994	In MCF-7 and PC3 cells, we measured the [Ca2+]i using Fura-2 fluorescence and observed a decrease in MCF-7 cells from 126 to 99 nM when the cells were exposed for 6 days to 45 microM suramin.	Fura-2	54-60	chromobox 8	Homo sapiens	13-16
8068023-1	1994	In single Fura-2 ester-loaded hepatocytes, stimulation by vasopressin, but not emptying of the agonist-sensitive Ca2+ store by 2,5-di-(t-butyl)hydroquinone, resulted in an increase in the rate of Fura-2 fluorescence-quenching by Mn2+.	Fura-2	10-16	arginine vasopressin	Homo sapiens	58-69
8068023-2	1994	Similarly, in cells microinjected with Fura-2 salt, vasopressin stimulated Mn2+ entry while 2,5-di-(t-butyl)hydroquinone or thapsigargin did not.	Fura-2	39-45	arginine vasopressin	Rattus norvegicus	52-63
7519011-11	1994	Both fMLP and thapsigargin stimulated quenching of Fura-2 fluorescence in the presence of extracellular Mn2+, whereas maitotoxin produced no Mn2+ quenching.	Fura-2	51-57	formyl peptide receptor 1	Homo sapiens	5-9
7982265-1	1994	Bradykinin (BDK)-induced increases in intracellular Ca2+ concentration ([Ca2+]i) were monitored in cultured canine tracheal smooth muscle cells (TSMCs) using a fluorescent Ca2+ indicator, Fura-2.	Fura-2	188-194	kininogen 1	Canis lupus familiaris	12-15
7519157-8	1994	Further, LIF treatment blocked the normal muscarinic receptor-mediated increase in intracellular calcium (fura-2 imaging), indicating a functional change in receptor phenotype.	Fura-2	106-112	LIF, interleukin 6 family cytokine	Rattus norvegicus	9-12
7953671-4	1994	Measurements of [Ca2+]i using fura-2 imaging revealed that the elevation of [Ca2+]i that occurred in response to long-term exposure to A beta was attenuated in neurons treated with staurosporine and K-252 compounds.	Fura-2	30-36	amyloid beta precursor protein	Rattus norvegicus	135-141
8026022-3	1994	METHODS AND RESULTS: Insulin (40 microU/mL) attenuated the 5-hydroxytryptamine (5-HT, serotonin; 10(-5) mol/L)-induced [Ca2+]i transient (measured by fura 2 fluorescence) in primary confluent canine femoral artery VSMCs in the presence of extracellular Ca2+.	Fura-2	150-156	insulin	Canis lupus familiaris	21-28
7944616-2	1994	METHODS: The release into the cytosol of Ca2+ from stores within individual neutrophils was determined using ratiometric imaging of fura2.	Fura-2	132-137	carbonic anhydrase 2	Homo sapiens	41-44
8026022-6	1994	In support of those conclusions, we found that insulin inhibited the 5-HT-induced component of Mn2+ (a Ca2+ surrogate) influx (measured by fura 2 fluorescence quenching at the Ca2+ isosbestic excitation wavelength).	Fura-2	139-145	insulin	Canis lupus familiaris	47-54
7932382-3	1994	The presence of functional receptors was shown by inositol trisphosphate accumulation and a rise in the concentration of intracellular free calcium evoked by platelet-activating factor in myo-[2-3H]inositol-labelled and fura-2-loaded cells, respectively.	Fura-2	220-226	PCNA clamp associated factor	Homo sapiens	158-184
7960031-3	1994	Using the microspectrofluorimetric technique with fura-2, as calcium indicator, brief applications of OT on single astrocytes induced a transient and reversible dose-dependent increase of intracellular calcium concentration ([Ca2+]i) in most of the cells tested.	Fura-2	50-56	oxytocin/neurophysin I prepropeptide	Homo sapiens	102-104
8021298-6	1994	Pretreatment of Fura 2 loaded cells with 4-bromophenacyl bromide, an inhibitor of PLA2 activity, decreased the thapsigargin-induced entry of calcium, suggesting a role for PLA2 in the regulation of calcium entry.	Fura-2	16-22	phospholipase A2 group IB	Rattus norvegicus	82-86
8021298-6	1994	Pretreatment of Fura 2 loaded cells with 4-bromophenacyl bromide, an inhibitor of PLA2 activity, decreased the thapsigargin-induced entry of calcium, suggesting a role for PLA2 in the regulation of calcium entry.	Fura-2	16-22	phospholipase A2 group IB	Rattus norvegicus	172-176
8069676-1	1994	Cd2+ and Co2+ at micromolar concentrations increased the cytosolic free Ca2+ concentration, which was measured by fura-2 microfluorometry, in cat adrenal chromaffin cells.	Fura-2	114-120	CD2 molecule	Homo sapiens	0-3
8082131-1	1994	Calcium release in response to the activation of muscarinic M1 and histamine H1 receptors was studied in single N1E-115 cells using Fura-2 imaging.	Fura-2	132-138	cholinergic receptor, muscarinic 1, CNS	Mus musculus	60-89
8206566-4	1994	Their manual and ambulatory automated 24-hour blood pressure measurements were correlated with cytosolic calcium in resting and stimulated (thrombin-treated) fura 2-loaded platelets.	Fura-2	158-164	coagulation factor II, thrombin	Homo sapiens	140-148
8072857-1	1994	Ca(2+)-mediated Ca2+ spikes were analysed in fura-2-loaded megakaryocytes.	Fura-2	45-51	carbonic anhydrase 2	Homo sapiens	16-19
7909692-1	1994	Acetoxymethyl ester (AM) derivatives of various fluorescent indicators (fura-2, fluo-3, indo-1, BCECF, calcein) are actively extruded by the multidrug transporter (MDR1, P-glycoprotein-Homolya, L. et al.	Fura-2	72-78	ATP binding cassette subfamily B member 1	Homo sapiens	164-168
8144609-4	1994	After loading the cytosol of intact hepatocytes with Mn2+, both submaximal and maximal vasopressin doses caused complete quench of the entire intracellular pool of compartmentalized fura-2.	Fura-2	182-188	arginine vasopressin	Homo sapiens	87-98
8164648-5	1994	Third, although the changes in intracellular Ca2+ of fura-2/AM-preloaded human neutrophils elicited by N51/KC and IL-8 are similar, pretreatment of the cells with N51/KC did not result in a loss of response to a subsequent treatment with IL-8; in contrast, treatment with IL-8 did result in the subsequent desensitization to N51/KC.	Fura-2	53-59	C-X-C motif chemokine ligand 8	Homo sapiens	114-118
8072693-9	1994	In Fura-2-loaded isolated sympathetic neurites obtained from superior cervical ganglia explants, the depolarization-induced (54 mM KCl) increase in cytosolic calcium was attenuated by neuropeptide Y (50 nM).	Fura-2	3-9	neuropeptide Y	Rattus norvegicus	184-198
8091392-2	1994	Basal and thrombin-induced increases in platelet intracellular free calcium ion concentration ([Ca2+]i; fura-2) were similar in hyperlipidemic patients and controls (45 +/- 5 vs 42 +/- 3 and 635 +/- 51 vs 599 +/- 69 mM, respectively).	Fura-2	104-110	coagulation factor II, thrombin	Homo sapiens	10-18
8190099-5	1994	The fMLP- or C5a-induced calcium mobilization form intracellular stores of fura-2-loaded cells, as well as the fMLP- or A23187-triggered release of [14C] arachidonate from prelabeled neutrophils, was not affected by B-003.	Fura-2	75-81	formyl peptide receptor 1	Homo sapiens	4-8
7512352-1	1994	Depolarization-induced intracellular Ca2+ rises were measured in fura-2-loaded, voltage-clamped Purkinje cells.	Fura-2	65-71	carbonic anhydrase 2	Homo sapiens	37-40
7545917-3	1994	Modulation of cellular phenotype by EMAP II-derived peptide was suggested by peptide-induced elevation of cytosolic free calcium concentration in fura-2-loaded MPs and PMNs and by stimulation of peroxidase release in PMNs.	Fura-2	146-152	aminoacyl tRNA synthetase complex interacting multifunctional protein 1	Homo sapiens	36-43
8132547-6	1994	Mn2+ induced a quench of the fura-2 fluorescence, which ceased promptly upon the removal of NPY, indicating that Ca2+ entry was linked tightly to receptor activation.	Fura-2	29-35	neuropeptide Y	Homo sapiens	92-95
7510965-5	1994	The effect of these two drugs upon (Ca2+)i changes was measured using the fluorescent probe fura-2 loaded into RBL-2H3 cells passively sensitized with DNP-specific IgE.	Fura-2	92-98	carbonic anhydrase 2	Rattus norvegicus	36-39
7510965-5	1994	The effect of these two drugs upon (Ca2+)i changes was measured using the fluorescent probe fura-2 loaded into RBL-2H3 cells passively sensitized with DNP-specific IgE.	Fura-2	92-98	immunoglobulin heavy constant epsilon	Homo sapiens	164-167
8117446-2	1994	In fura-2-loaded cells, PAF (10(-9) to 10(-5) M), but not lyso-PAF, increased [Ca2+]i in a concentration-dependent manner, from 106 +/- 15 to 270 +/- 40 nM (P < 0.05).	Fura-2	3-9	PCNA-associated factor	Bos taurus	24-27
8187959-1	1994	Luteinizing hormone (LH)/chorionic gonadotropin (CG) receptor (LHR) regulation of intracellular free Ca2+, [Ca2+]i, was studied by spectrofluorometric analysis and digital imaging of intracellularly trapped fura-2 fluorescence in a stable cell line 293 expressing transfected rat LHR.	Fura-2	207-213	chorionic gonadotropin subunit beta 5	Homo sapiens	49-51
8149415-3	1994	Pretreatment of Fura-2 loaded cells with 4-bromophenylacyl, an inhibitor of phospholipase A2, had no effect on the ATP-induced entry of Ca2+ but inhibited the release of sequestered Ca2+.	Fura-2	16-22	phospholipase A2 group IB	Rattus norvegicus	76-92
8293556-7	1994	By use of Mn2+ quenching of intracellular fura 2 to measure divalent cation entry, the maximal rate of vasopressin-stimulated Mn2+ entry was impaired in simvastatin-treated cells by 52%.	Fura-2	42-48	arginine vasopressin	Rattus norvegicus	103-114
7513035-4	1994	In separate experiments, [Ca2+]i changes in response to IGF-I were measured spectrofluorometrically in fura-2-loaded cells.	Fura-2	103-109	insulin like growth factor 1	Homo sapiens	56-61
8187959-1	1994	Luteinizing hormone (LH)/chorionic gonadotropin (CG) receptor (LHR) regulation of intracellular free Ca2+, [Ca2+]i, was studied by spectrofluorometric analysis and digital imaging of intracellularly trapped fura-2 fluorescence in a stable cell line 293 expressing transfected rat LHR.	Fura-2	207-213	luteinizing hormone/choriogonadotropin receptor	Rattus norvegicus	63-66
8275927-2	1994	Video image analysis of fura-2-loaded cells revealed that PTH (10(-7) M) induced transient increases of [Ca2+]i preferentially in cells in S phase (82% response frequency, n = 63; 286 +/- 33% of baseline, n = 29), whereas cells in G1 phase responded poorly to PTH (10% response frequency, n = 51; 140 +/- 8% of baseline, n = 5).	Fura-2	24-30	parathyroid hormone	Rattus norvegicus	58-61
8293807-1	1994	Regulation of the cytoplasmic calcium concentration ([Ca2+]i) was studied in fura-2-loaded C-cells from two human medullary thyroid carcinomas (MTC).	Fura-2	77-83	carbonic anhydrase 2	Homo sapiens	54-57
8124662-1	1994	Angiotensin II (Ang II), bradykinin (BK), and endothelin-1 (ET-1) evoked alterations in cytosolic free calcium, [Ca2+]i, levels were determined using fura-2 fluorescence methodology in a human lung adenocarcinoma cell line (A549), a non-neoplastic lung cell line and a small cell lung carcinoma cell (SCLC) line.	Fura-2	150-156	angiotensinogen	Homo sapiens	0-14
8124662-1	1994	Angiotensin II (Ang II), bradykinin (BK), and endothelin-1 (ET-1) evoked alterations in cytosolic free calcium, [Ca2+]i, levels were determined using fura-2 fluorescence methodology in a human lung adenocarcinoma cell line (A549), a non-neoplastic lung cell line and a small cell lung carcinoma cell (SCLC) line.	Fura-2	150-156	endothelin 1	Homo sapiens	46-58
8124662-1	1994	Angiotensin II (Ang II), bradykinin (BK), and endothelin-1 (ET-1) evoked alterations in cytosolic free calcium, [Ca2+]i, levels were determined using fura-2 fluorescence methodology in a human lung adenocarcinoma cell line (A549), a non-neoplastic lung cell line and a small cell lung carcinoma cell (SCLC) line.	Fura-2	150-156	endothelin 1	Homo sapiens	60-64
8275927-2	1994	Video image analysis of fura-2-loaded cells revealed that PTH (10(-7) M) induced transient increases of [Ca2+]i preferentially in cells in S phase (82% response frequency, n = 63; 286 +/- 33% of baseline, n = 29), whereas cells in G1 phase responded poorly to PTH (10% response frequency, n = 51; 140 +/- 8% of baseline, n = 5).	Fura-2	24-30	parathyroid hormone	Rattus norvegicus	260-263
8300340-4	1994	Endothelin receptor linkage to cell signaling pathways was determined based on measurements of the dose dependent effects of ET-1, ET-2, and ET-3 on intracellular Ca2+ concentration ([Ca2+]i) transients in fura-2-loaded cells, and of ET-1 on phosphoinositide turnover and cAMP accumulation in the isolated rabbit corneal epithelium.	Fura-2	206-212	endothelin-1	Oryctolagus cuniculus	125-129
8001869-5	1994	Interleukin-1 beta (IL-1 beta) and tumor necrosis factor-beta (TNF-beta) led to a significant increase in relative fluorescence release (RFR) of fura-2 (p < 0.05 and p < 0.02, respectively) while endotoxin did not show significant changes of RFR of fura-2.	Fura-2	145-151	interleukin 1 beta	Homo sapiens	0-18
8001869-5	1994	Interleukin-1 beta (IL-1 beta) and tumor necrosis factor-beta (TNF-beta) led to a significant increase in relative fluorescence release (RFR) of fura-2 (p < 0.05 and p < 0.02, respectively) while endotoxin did not show significant changes of RFR of fura-2.	Fura-2	145-151	interleukin 1 beta	Homo sapiens	20-29
8001869-5	1994	Interleukin-1 beta (IL-1 beta) and tumor necrosis factor-beta (TNF-beta) led to a significant increase in relative fluorescence release (RFR) of fura-2 (p < 0.05 and p < 0.02, respectively) while endotoxin did not show significant changes of RFR of fura-2.	Fura-2	145-151	lymphotoxin alpha	Homo sapiens	35-61
8001869-5	1994	Interleukin-1 beta (IL-1 beta) and tumor necrosis factor-beta (TNF-beta) led to a significant increase in relative fluorescence release (RFR) of fura-2 (p < 0.05 and p < 0.02, respectively) while endotoxin did not show significant changes of RFR of fura-2.	Fura-2	145-151	lymphotoxin alpha	Homo sapiens	63-71
8001869-5	1994	Interleukin-1 beta (IL-1 beta) and tumor necrosis factor-beta (TNF-beta) led to a significant increase in relative fluorescence release (RFR) of fura-2 (p < 0.05 and p < 0.02, respectively) while endotoxin did not show significant changes of RFR of fura-2.	Fura-2	255-261	interleukin 1 beta	Homo sapiens	0-18
8001869-5	1994	Interleukin-1 beta (IL-1 beta) and tumor necrosis factor-beta (TNF-beta) led to a significant increase in relative fluorescence release (RFR) of fura-2 (p < 0.05 and p < 0.02, respectively) while endotoxin did not show significant changes of RFR of fura-2.	Fura-2	255-261	interleukin 1 beta	Homo sapiens	20-29
8001869-5	1994	Interleukin-1 beta (IL-1 beta) and tumor necrosis factor-beta (TNF-beta) led to a significant increase in relative fluorescence release (RFR) of fura-2 (p < 0.05 and p < 0.02, respectively) while endotoxin did not show significant changes of RFR of fura-2.	Fura-2	255-261	lymphotoxin alpha	Homo sapiens	35-61
8001869-5	1994	Interleukin-1 beta (IL-1 beta) and tumor necrosis factor-beta (TNF-beta) led to a significant increase in relative fluorescence release (RFR) of fura-2 (p < 0.05 and p < 0.02, respectively) while endotoxin did not show significant changes of RFR of fura-2.	Fura-2	255-261	lymphotoxin alpha	Homo sapiens	63-71
8300340-4	1994	Endothelin receptor linkage to cell signaling pathways was determined based on measurements of the dose dependent effects of ET-1, ET-2, and ET-3 on intracellular Ca2+ concentration ([Ca2+]i) transients in fura-2-loaded cells, and of ET-1 on phosphoinositide turnover and cAMP accumulation in the isolated rabbit corneal epithelium.	Fura-2	206-212	endothelin-2	Oryctolagus cuniculus	131-135
8300340-4	1994	Endothelin receptor linkage to cell signaling pathways was determined based on measurements of the dose dependent effects of ET-1, ET-2, and ET-3 on intracellular Ca2+ concentration ([Ca2+]i) transients in fura-2-loaded cells, and of ET-1 on phosphoinositide turnover and cAMP accumulation in the isolated rabbit corneal epithelium.	Fura-2	206-212	endothelin-3	Oryctolagus cuniculus	141-145
8300340-4	1994	Endothelin receptor linkage to cell signaling pathways was determined based on measurements of the dose dependent effects of ET-1, ET-2, and ET-3 on intracellular Ca2+ concentration ([Ca2+]i) transients in fura-2-loaded cells, and of ET-1 on phosphoinositide turnover and cAMP accumulation in the isolated rabbit corneal epithelium.	Fura-2	206-212	endothelin-1	Oryctolagus cuniculus	234-238
7506746-3	1994	The elevation in cytosolic calcium, as detected in Fura-2-labeled basophils, after challenge with anti-IgE or fMLP showed graded characteristics in that the distributions were unimodal under conditions of optimal or suboptimal challenge with little skewing from a normal distribution.	Fura-2	51-57	formyl peptide receptor 1	Homo sapiens	110-114
8115999-1	1993	The effect of type IIB von Willebrand"s factor (vWF) on platelet cytosolic Ca2+ ion concentration, measured by means of the probe fura 2, was investigated.	Fura-2	130-136	von Willebrand factor	Homo sapiens	48-51
7507268-8	1994	Fura 2 was used to measure intracellular free Cd2+ by fluorescence ratio methods.	Fura-2	0-6	Cd2 molecule	Rattus norvegicus	46-49
7507268-10	1994	Cd2+ addition resulted in a gradual increase in Fura 2 fluorescence in both PC12 and PC18 cells, but depolarization with BAY K8644 increased the apparent rate of Cd2+ uptake only for the PC12 cells.	Fura-2	48-54	Cd2 molecule	Rattus norvegicus	0-3
8087627-3	1993	Therefore, we studied the effect of BK, and its putative modulation by tumor necrosis factor alpha (TNF alpha), on cytosolic free Ca2+ concentration ([Ca2+]i) in fura-2-loaded tracheal smooth muscle cells in culture.	Fura-2	162-168	tumor necrosis factor	Cavia porcellus	100-109
8145147-12	1993	In fura-2-loaded unclamped cells, TRH induced either release of Ca2+ from internal stores, Ca2+ entry, or both.	Fura-2	3-9	thyrotropin releasing hormone	Cavia porcellus	34-37
8119327-2	1993	Endothelin-3 in a range of 0.1-100 pM induced arteriolar constriction in a dose-dependent manner, and stimulated Ca2+ mobilization, demonstrated by fura-2-associated fluorography, in both arterioles and venules.	Fura-2	148-154	endothelin 3	Rattus norvegicus	0-12
8120825-11	1993	When the internal concentration of calcium ions, [Ca2+]i, was measured using fura-2, brief transmural stimuli caused an increase in [Ca2+]i.	Fura-2	77-83	LOW QUALITY PROTEIN: carbonic anhydrase 2	Cavia porcellus	50-53
8120825-11	1993	When the internal concentration of calcium ions, [Ca2+]i, was measured using fura-2, brief transmural stimuli caused an increase in [Ca2+]i.	Fura-2	77-83	LOW QUALITY PROTEIN: carbonic anhydrase 2	Cavia porcellus	133-136
7997069-4	1994	The results in vitro using the Ca(2+)-sensitive dye fura-2 showed that nimodipine reduced in a dose-dependent manner the elevation of [Ca2+]i in hippocampal neurons induced by K(+)-stimulation.	Fura-2	52-58	carbonic anhydrase 2	Rattus norvegicus	135-138
8262215-4	1993	Additionally, extracellular application of Ba2+ increased the apparent internal Ca2+ concentration ([Ca2+]app) in fura-2-loaded chromaffin cells.	Fura-2	114-120	carbonic anhydrase 2	Bos taurus	80-83
8262215-4	1993	Additionally, extracellular application of Ba2+ increased the apparent internal Ca2+ concentration ([Ca2+]app) in fura-2-loaded chromaffin cells.	Fura-2	114-120	carbonic anhydrase 2	Bos taurus	101-104
8262215-11	1993	In some cases, Ba2+ additionally induces release of Ca2+ from internal stores, as evidenced by its effect on fura-2 fluorescence at different wavelengths.	Fura-2	109-115	carbonic anhydrase 2	Bos taurus	52-55
8227034-4	1993	Studies in fura-2/AM-loaded cells showed that concentrations of thapsigargin that elicited PAF production induced a protracted and long lasting elevation of cytosolic free calcium concentration ([Ca2+]i) between 200 and 700 nM.	Fura-2	11-17	PCNA clamp associated factor	Homo sapiens	91-94
8288312-8	1993	Fura-2 analysis demonstrated that PMA-induced PKC translocation was not accompanied by a net change in cytosolic calcium levels.	Fura-2	0-6	proline rich transmembrane protein 2	Homo sapiens	46-49
8077313-2	1993	In the present study we used fura-2 microfluorimetry to investigate whether activation of the oxytocin receptor of cultured human granulosa-lutein cells causes intracellular calcium (Ca2+) signals and affects progesterone release.	Fura-2	29-35	oxytocin/neurophysin I prepropeptide	Homo sapiens	94-102
8226749-3	1993	[Ca2+]c was monitored by video imaging of single fura-2-loaded INS-1 cells, or in populations of cells transfected with non-targeted (cytosolic) aequorin.	Fura-2	49-55	insulin 1	Rattus norvegicus	63-68
8407906-2	1993	Single cell intracellular Ca2+ responses to Pg and u-PA were studied using Fura-2 and digital imaging fluorescence microscopy.	Fura-2	75-81	plasminogen activator, urokinase	Homo sapiens	51-55
7688740-0	1993	ADP- and thapsigargin-evoked Ca2+ entry and protein-tyrosine phosphorylation are inhibited by the tyrosine kinase inhibitors genistein and methyl-2,5-dihydroxycinnamate in fura-2-loaded human platelets.	Fura-2	172-178	WD and tetratricopeptide repeats 1	Homo sapiens	0-4
8247351-5	1993	Intracellular Ca2+ levels were monitored using the Ca2+ indicator Fura-2 and were found to rise in both hair cell types in response to ATP.	Fura-2	66-72	LOW QUALITY PROTEIN: carbonic anhydrase 2	Cavia porcellus	14-17
8247351-5	1993	Intracellular Ca2+ levels were monitored using the Ca2+ indicator Fura-2 and were found to rise in both hair cell types in response to ATP.	Fura-2	66-72	LOW QUALITY PROTEIN: carbonic anhydrase 2	Cavia porcellus	51-54
8376384-2	1993	We report that CCK-JMV-180 can accelerate Ca2+ influx into fura-2-loaded dispersed rat pancreatic acini and single acinar cells.	Fura-2	59-65	cholecystokinin	Rattus norvegicus	15-18
8357814-0	1993	Fura-2 transport in toad urinary bladder epithelium: effects of antidiuretic hormone, colchicine and osmotic gradients.	Fura-2	0-6	arginine vasopressin	Homo sapiens	64-84
8240466-9	1993	Studies with the fluorescent calcium chelating dye FURA 2/AM showed that nimesulide was able to reduce free cytosolic calcium increase produced by fMLP and the ionophore ionomycin.	Fura-2	51-57	formyl peptide receptor 1	Homo sapiens	147-151
8220881-2	1993	In Fura-2 preloaded human platelets, the increase in cytosolic calcium induced by alpha-thrombin was reduced by some L- and D-arginine ester compounds the IC50 (microM) values of which were 7.4 for TAEE, 56.9 for BAEE, 77.6 for TAME, 560 for T(d)AME, 656.3 for L-ArgOMe and 2206.7 for D-ArgOMe.	Fura-2	3-9	coagulation factor II, thrombin	Homo sapiens	88-96
8255728-5	1993	The net electrochemical gradient for ionic movements through the Na+/Ca2+ exchanger, during the course of the response of macrophages to PAF, was determined by combining the measurements of membrane potential (in patch-clamp), of [Ca2+]i (with fura-2), and of the intracellular Na+ concentration (with sodium-binding benzofuran isophthalate).	Fura-2	244-250	solute carrier family 8 member A1	Homo sapiens	65-83
8394831-2	1993	Activin A increased intracellular the free Ca2+ concentration, [Ca2+]i in fura-2-loaded islets.	Fura-2	74-80	inhibin subunit beta A	Rattus norvegicus	0-9
8368272-3	1993	Changes in pHi and Cai were measured with the fluorescent probes 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein and fura 2.	Fura-2	116-122	glucose-6-phosphate isomerase	Rattus norvegicus	11-14
7686480-7	1993	Digital fluorescence imaging of individual fura-2-loaded cells confirmed that the average [Ca2+]i response to depolarization was lower in cells that had been incubated with EGF for 36 h. EGF treatment increased the amount of PRL secreted basally, but inhibited the acute PRL secretory response to depolarization with 50 mM KCl and 1 microM BAY K8644 from 2.6- to 1.5-fold.	Fura-2	43-49	epidermal growth factor like 1	Rattus norvegicus	173-176
8394074-5	1993	Additional studies in which the extracellular and intracellular concentrations of Ca2+ were varied indicated that maximal LTB4-induced PLD activation was dependent upon Ca2+ and potentiated by inhibitors of protein kinase C. The time-course and concentration-response curves for LTB4-induced PLD activation were different from those for LTB4-induced Ca2+ mobilization, as measured by fura-2 fluorescence.	Fura-2	384-390	glycosylphosphatidylinositol specific phospholipase D1	Homo sapiens	135-138
8252582-4	1993	METHODS: The effect of erythropoietin on ([Ca2+]i was measured with the fluorescent dye fura2 in cultured vascular smooth muscle cells from Wistar Kyoto rats.	Fura-2	88-93	erythropoietin	Rattus norvegicus	23-37
8319379-3	1993	We found that purinoceptor agonists, including ATP and its analogs, and bombesin, an amphibian tetradeca-peptide of mammalian homology gastrin-releasing peptide, induced rapid transient increase of cytoplasmic-free Ca2+ in PG cells loaded with fura-2.	Fura-2	244-250	gastrin releasing peptide	Homo sapiens	72-80
7686480-7	1993	Digital fluorescence imaging of individual fura-2-loaded cells confirmed that the average [Ca2+]i response to depolarization was lower in cells that had been incubated with EGF for 36 h. EGF treatment increased the amount of PRL secreted basally, but inhibited the acute PRL secretory response to depolarization with 50 mM KCl and 1 microM BAY K8644 from 2.6- to 1.5-fold.	Fura-2	43-49	epidermal growth factor like 1	Rattus norvegicus	187-190
7684899-5	1993	In addition, SP-(7-11), but not intact SP, SP-(1-4), SP-(1-6), SP-(8-11) or SP-(9-11), nor the tachykinins NKA and NKB, caused an increase in the intracellular calcium concentration as measured by the fluorescent dye Fura-2.	Fura-2	217-223	tachykinin precursor 1	Bos taurus	13-15
8390453-1	1993	Emptying the intracellular calcium stores of fura-2-loaded human neutrophils by treatment with the endomembrane ATPase inhibitor thapsigargin leads to a maintained increase of [Ca2+]i by Ca2+ entry through a store-operated Ca2+ entry pathway.	Fura-2	45-51	dynein axonemal heavy chain 8	Homo sapiens	112-118
8395337-1	1993	Free calcium concentration in isolated single neurons was clamped using a new technical approach based on a feed-back connection between the Fura-2 fluorescence signal measuring the intracellular Ca2+ concentration ([Ca2+]i) and iontophoretic current injecting Ca2+ into the cell.	Fura-2	141-147	carbonic anhydrase 2	Homo sapiens	196-199
8395337-1	1993	Free calcium concentration in isolated single neurons was clamped using a new technical approach based on a feed-back connection between the Fura-2 fluorescence signal measuring the intracellular Ca2+ concentration ([Ca2+]i) and iontophoretic current injecting Ca2+ into the cell.	Fura-2	141-147	carbonic anhydrase 2	Homo sapiens	217-220
8395337-1	1993	Free calcium concentration in isolated single neurons was clamped using a new technical approach based on a feed-back connection between the Fura-2 fluorescence signal measuring the intracellular Ca2+ concentration ([Ca2+]i) and iontophoretic current injecting Ca2+ into the cell.	Fura-2	141-147	carbonic anhydrase 2	Homo sapiens	217-220
8411123-3	1993	We investigated PAF-induced Ca2+ signaling in HMDMs using the calcium indicator Fura-2, combining single cell ratio fluorimetry and digital video imaging with whole-cell recording techniques.	Fura-2	80-86	PCNA clamp associated factor	Homo sapiens	16-19
8335062-2	1993	In this paper, changes in intracellular Ca2+ concentration ([Ca2+]i) in response to CCK (cholecystokinin) peptides were measured in the Human JURKAT T Lymphocyte Cell line by fura-2 fluorometry.	Fura-2	175-181	cholecystokinin	Homo sapiens	84-87
8335062-2	1993	In this paper, changes in intracellular Ca2+ concentration ([Ca2+]i) in response to CCK (cholecystokinin) peptides were measured in the Human JURKAT T Lymphocyte Cell line by fura-2 fluorometry.	Fura-2	175-181	cholecystokinin	Homo sapiens	89-104
8397354-1	1993	Intracellular calcium levels were measured by ratio imaging of Fura-2-injected CA1 pyramidal neurons during repetitive electrical stimulation of the Schaffer collaterals in rat hippocampal slices.	Fura-2	63-69	carbonic anhydrase 1	Rattus norvegicus	79-82
8261130-7	1993	Combined voltage-clamp recordings and microfluorometric measurements of [Ca2+]i using the Ca2+ indicator fura-2 revealed that the amplitude of IADP was correlated with the amplitude of depolarization-induced Ca2+ influx.	Fura-2	105-111	carbonic anhydrase 2	Rattus norvegicus	73-76
8261130-7	1993	Combined voltage-clamp recordings and microfluorometric measurements of [Ca2+]i using the Ca2+ indicator fura-2 revealed that the amplitude of IADP was correlated with the amplitude of depolarization-induced Ca2+ influx.	Fura-2	105-111	carbonic anhydrase 2	Rattus norvegicus	90-93
8261130-7	1993	Combined voltage-clamp recordings and microfluorometric measurements of [Ca2+]i using the Ca2+ indicator fura-2 revealed that the amplitude of IADP was correlated with the amplitude of depolarization-induced Ca2+ influx.	Fura-2	105-111	carbonic anhydrase 2	Rattus norvegicus	90-93
8386174-3	1993	Fluorescence measurements using Fura-2 show that at pH 7.4, the Ca2+ requirement for gelsolin activation in vitro is higher than previously reported, with half-maximal activation of severing and nucleation occurring at 10 microM Ca2+.	Fura-2	32-38	gelsolin	Homo sapiens	85-93
8392939-2	1993	Fura-2-loaded and gel-filtered platelets were stimulated by either ADP (8 microM), thrombin (0.025 IU/ml) or collagen (1-3 micrograms/ml), respectively.	Fura-2	0-6	coagulation factor II, thrombin	Homo sapiens	83-91
8393065-5	1993	The intracellular [Ca2+] (measured with fura-2) was significantly increased, from 125 nM (control) to 335 nM (0 mM K+ bath) or 408 nM (0.2 mM strophanthidin), during inhibition of pump activity.	Fura-2	40-46	carbonic anhydrase 2	Rattus norvegicus	19-22
8329674-1	1993	The isolated rat renal arteriole technique was adapted for use in a fluorescence ratio imaging system in which angiotensin II (AII)-induced changes in lumen diameter and smooth muscle cell (SMC) cytosolic calcium [(Ca2+]i were serially determined for 4 min with Fura-2.	Fura-2	262-268	angiotensinogen	Rattus norvegicus	111-125
8329674-1	1993	The isolated rat renal arteriole technique was adapted for use in a fluorescence ratio imaging system in which angiotensin II (AII)-induced changes in lumen diameter and smooth muscle cell (SMC) cytosolic calcium [(Ca2+]i were serially determined for 4 min with Fura-2.	Fura-2	262-268	angiotensinogen	Rattus norvegicus	127-130
8387925-5	1993	Fluorescent ratio imaging with fura-2 revealed that addition of vasopressin to the cells results in an increase of [Ca2+]i which peaks within 20 s and does not return to resting levels during the 100 s observation period.	Fura-2	31-37	arginine vasopressin	Rattus norvegicus	64-75
8491259-1	1993	The rare earth lanthanide gadolinium (Gd3+), in concentrations ranging from 1 to 100 microM, reduced the elevation of intracellular Ca2+ concentration [Ca2+]i, monitored by means of the fluorescent probe fura-2.	Fura-2	204-210	GRDX	Homo sapiens	38-41
8384995-4	1993	When the intracellular free Ca2+ concentration ([Ca2+]i) is measured in a fura 2-loaded single chromaffin cell, PACAP is found to cause a sustained increase in [Ca2+]i by mobilizing Ca2+ from both extra- and intracellular pools.	Fura-2	74-80	adenylate cyclase activating polypeptide 1	Homo sapiens	112-117
7504109-4	1993	The amount of intracellular free Ca2+ ([Ca2+]i) was fluorometrically measured using fura-2.	Fura-2	84-90	carbonic anhydrase 2	Rattus norvegicus	33-36
7504109-4	1993	The amount of intracellular free Ca2+ ([Ca2+]i) was fluorometrically measured using fura-2.	Fura-2	84-90	carbonic anhydrase 2	Rattus norvegicus	40-43
7504109-20	1993	Fura-2 imaging showed that [Ca2+]i was increased when ACh was added to the external medium with or without Ca2+, though in Ca(2+)-free medium only the first application of ACh increased the [Ca2+]i.	Fura-2	0-6	carbonic anhydrase 2	Rattus norvegicus	28-31
7504109-20	1993	Fura-2 imaging showed that [Ca2+]i was increased when ACh was added to the external medium with or without Ca2+, though in Ca(2+)-free medium only the first application of ACh increased the [Ca2+]i.	Fura-2	0-6	carbonic anhydrase 2	Rattus norvegicus	107-110
7504109-20	1993	Fura-2 imaging showed that [Ca2+]i was increased when ACh was added to the external medium with or without Ca2+, though in Ca(2+)-free medium only the first application of ACh increased the [Ca2+]i.	Fura-2	0-6	carbonic anhydrase 2	Rattus norvegicus	107-110
8382636-2	1993	The flux through ROCCs was followed by quenching of fura-2 fluorescence due to the influx of extracellular Mn2+ induced by vasopressin.	Fura-2	52-58	arginine vasopressin	Rattus norvegicus	123-134
8384417-10	1993	Cai was measured using the fluorescence of the Ca indicator fura-2 in principal cells of split tubules under conditions identical to those used for electrical measurements.	Fura-2	60-66	carbonic anhydrase 1	Rattus norvegicus	0-3
7679080-0	1993	Calcium influx through annexin V ion channels into large unilamellar vesicles measured with fura-2.	Fura-2	92-98	annexin A5	Homo sapiens	23-32
8383969-2	1993	[Ca2+]i and pHi were measured using the fluorescent dyes fura-2 and BCECF [2",7"-bis-(carboxyethyl)-5,6-carboxyfluorescein] respectively.	Fura-2	57-63	glucose-6-phosphate isomerase	Homo sapiens	12-15
8445010-5	1993	Preincubation of hirudinized platelet-rich plasma with insulin had a dose- and time-dependent effect on the increase of [Mg2+]i measured in Mag-fura-2-loaded cells with a fluorescence spectrophotometer.	Fura-2	144-150	insulin	Homo sapiens	55-62
8382265-3	1993	FTX (1:4,000 dilution, with respect to the original fraction) inhibited K(+)-depolarization-induced Ca2+ influx by 50%, as monitored with fura-2, whereas nitrendipine (0.1-1 microM) and FTX (3:3), a synthetic FTX analogue (1 mM), blocked the [Ca2+]i transients by 35 and 30%, respectively.	Fura-2	138-144	FTX transcript, XIST regulator	Homo sapiens	0-3
8510321-1	1993	This study was aimed at determining whether the thrombin (TRB)-induced aggregation and intracellular free Ca2+ ([Ca(2+)]i) mobilization of fura-2-loaded human platelets would be affected by aminoglycoside antibiotics (AGs), i.e., kanamycin (KM), gentamicin (GM) and fradiomycin (FRM) with 4, 5 and 6 amino groups in their molecules, respectively.	Fura-2	139-145	coagulation factor II, thrombin	Homo sapiens	48-56
8510321-1	1993	This study was aimed at determining whether the thrombin (TRB)-induced aggregation and intracellular free Ca2+ ([Ca(2+)]i) mobilization of fura-2-loaded human platelets would be affected by aminoglycoside antibiotics (AGs), i.e., kanamycin (KM), gentamicin (GM) and fradiomycin (FRM) with 4, 5 and 6 amino groups in their molecules, respectively.	Fura-2	139-145	coagulation factor II, thrombin	Homo sapiens	58-61
7679430-3	1993	Agonist-stimulated Ca2+ influx was demonstrated directly by the use of Mn2+: in the presence of extracellular Mn2+, MCP-1 and FMLP stimulated a dose-dependent quench in fluorescence of Fura-2-loaded monocytes.	Fura-2	185-191	C-C motif chemokine ligand 2	Homo sapiens	116-121
7679430-3	1993	Agonist-stimulated Ca2+ influx was demonstrated directly by the use of Mn2+: in the presence of extracellular Mn2+, MCP-1 and FMLP stimulated a dose-dependent quench in fluorescence of Fura-2-loaded monocytes.	Fura-2	185-191	formyl peptide receptor 1	Homo sapiens	126-130
7679080-4	1993	The resulting calcium influx into the LUV detected through the fluorescence changes of fura-2 was used as a qualitative test for the electrophysiological properties of annexin V.	Fura-2	87-93	annexin A5	Homo sapiens	168-177
8419539-5	1993	Experiments of [Ca2+]i measurement with the fura-2 technique showed that P2y agonists, as well as bradykinin, were able to increase [Ca2+]i, this effect being independent of the presence of extracellular Ca2+.	Fura-2	44-50	kininogen 1	Homo sapiens	98-108
8435086-17	1993	Finally, use of fura-2 and SK&F 96365 to manipulate the fMLP-stimulated rise in [Ca2+]i showed that when fMLP was able to evoke its normal rise in [Ca2+]i (to a peak of 700-900 nM), O2-.	Fura-2	16-22	formyl peptide receptor 1	Homo sapiens	60-64
8435086-17	1993	Finally, use of fura-2 and SK&F 96365 to manipulate the fMLP-stimulated rise in [Ca2+]i showed that when fMLP was able to evoke its normal rise in [Ca2+]i (to a peak of 700-900 nM), O2-.	Fura-2	16-22	formyl peptide receptor 1	Homo sapiens	109-113
7679387-1	1993	Intracellular calcium ([Ca2+]i) was measured in FURA 2-loaded endothelial cells plated on fibronectin or vitronectin.	Fura-2	48-54	fibronectin 1	Homo sapiens	90-101
7679387-1	1993	Intracellular calcium ([Ca2+]i) was measured in FURA 2-loaded endothelial cells plated on fibronectin or vitronectin.	Fura-2	48-54	vitronectin	Homo sapiens	105-116
8450563-7	1993	As detected by single cell microfluorimetric (fura-2) measurements neuropeptide Y induced an increase of [Ca2+]i which was caused by the entry of extracellular Ca2+.	Fura-2	46-52	neuropeptide Y	Rattus norvegicus	67-81
8275798-1	1993	Platelet-activating factor (PAF) elicited an increase in intracellular Ca2+ concentration, [Ca2+]i, in neuroblastoma x glioma hybrid NG 108-15 cells as measured by fura-2 fluorescence method.	Fura-2	164-170	patchy fur	Mus musculus	0-26
8422259-1	1993	Incubating Fura 2 loaded thyroid FRTL-5 cells with the calmodulin inhibitor W-7 decreased the ATP-evoked increase in intracellular free calcium.	Fura-2	11-17	calmodulin 1	Rattus norvegicus	55-65
8422913-3	1993	Here we have investigated the effects of two tyrosine kinase inhibitors, methyl 2,5-dihydroxycinnamate and genistein, on thrombin-evoked protein tyrosine phosphorylation and Ca2+ signal generation in fura-2-loaded human platelets.	Fura-2	200-206	coagulation factor II, thrombin	Homo sapiens	121-129
8420790-3	1993	Gel-filtered and fura-2-loaded platelets were stimulated with ADP (4-8 microM), collagen (2-10 micrograms/ml) or thrombin (0.02-0.05 IU/ml), respectively.	Fura-2	17-23	coagulation factor II, thrombin	Homo sapiens	113-121
8275798-1	1993	Platelet-activating factor (PAF) elicited an increase in intracellular Ca2+ concentration, [Ca2+]i, in neuroblastoma x glioma hybrid NG 108-15 cells as measured by fura-2 fluorescence method.	Fura-2	164-170	patchy fur	Mus musculus	28-31
1471987-2	1992	The [Ca2+]i rises elicited by low (50-80 nM) cathepsin G concentrations in fura-2-loaded platelets showed a biphasic mode, with a first small peak followed by a greater and more prolonged Ca2+ transient.	Fura-2	75-81	cathepsin G	Homo sapiens	45-56
1361128-1	1992	Changes in intracellular Ca2+ concentration ([Ca2+]i) in the soma and dendrites of hippocampal CA1 pyramidal neurons were measured using intracellularly injected fura-2.	Fura-2	162-168	carbonic anhydrase 1	Homo sapiens	95-98
1336985-4	1992	It also reduced the size of the PAF-induced calcium (Ca2+) transient demonstrated in Fura-2 loaded platelets.	Fura-2	85-91	PCNA clamp associated factor	Homo sapiens	32-35
1327522-3	1992	We found that the addition of gastrin to small cell lung cancer cells loaded with the fluorescent Ca2+ indicator fura 2-tetraacetoxymethylester causes a rapid and transient increase in the intracellular concentration of Ca2+ ([Ca2+]i) followed by homologous desensitization.	Fura-2	113-119	gastrin	Homo sapiens	30-37
1491443-5	1992	Fura-2/AM-loaded nephron segments reveal a transient increase of cytosolic free calcium concentrations by agonists such as angiotensin II, vasopressin, kinins, etc.	Fura-2	0-6	angiotensinogen	Homo sapiens	123-137
1491443-5	1992	Fura-2/AM-loaded nephron segments reveal a transient increase of cytosolic free calcium concentrations by agonists such as angiotensin II, vasopressin, kinins, etc.	Fura-2	0-6	arginine vasopressin	Homo sapiens	139-150
1332716-6	1992	Thus lipoproteins, like Ang II and PDGF-BB, activate polyphosphatidylinositol-specific phospholipase C. Intracellular Ca2+ concentrations ([Ca2+]i) were studied in fura-2 loaded VSMC.	Fura-2	164-170	angiotensinogen	Homo sapiens	24-30
1400356-2	1992	To elucidate the effects of endothelium-derived relaxing factor (EDRF) released from vascular endothelial cells (ECs) on handling of intracellular calcium ion (Ca2+i) in ECs themselves and vascular smooth muscle cells (VSMCs), we measured the Ca2+i by two-dimensional digital image analysis of fura-2-loaded ECs and VSMCs in tissue culture.	Fura-2	294-300	alpha hemoglobin stabilizing protein	Homo sapiens	65-69
1331121-1	1992	We have previously reported that platelet-activating factor (PAF) elevates cytosolic free calcium concentration ([Ca2+]i) in fura-2-loaded glomerular mesangial cells.	Fura-2	125-131	PCNA clamp associated factor	Rattus norvegicus	33-59
1331121-1	1992	We have previously reported that platelet-activating factor (PAF) elevates cytosolic free calcium concentration ([Ca2+]i) in fura-2-loaded glomerular mesangial cells.	Fura-2	125-131	PCNA clamp associated factor	Rattus norvegicus	61-64
1335472-9	1992	When the relationship between pHi and intracellular Ca2+ was determined with the fluorescent Ca2+ indicator, Fura-2, it was found that increasing pHo and treatment with ammonium chloride increased intracellular Ca2+, while sodium propionate and reducing pHi had no effect on intracellular Ca2+.	Fura-2	109-115	glucose-6-phosphate isomerase	Rattus norvegicus	30-33
1359030-2	1992	We have used microfluorimetry of the calcium indicator fura-2 (Grynkiewicz et al., 1985) to measure and characterize synaptically activated calcium changes in individual CA1 pyramidal cells contained within guinea pig hippocampal slices.	Fura-2	55-61	carbonic anhydrase 1	Cavia porcellus	170-173
1440710-3	1992	METHODS: The fluorescent indicators fura-2 and bis-carboxyethyl-carboxyfluorescein were used to simultaneously measure [Ca2+]i and pHi with a fluorometer.	Fura-2	36-42	glucose-6-phosphate isomerase	Rattus norvegicus	131-134
1362150-1	1992	Changes in the membrane potential and the intracellular Ca2+ concentration ([Ca2+]i) caused by somatostatin (SRIF) were simultaneously measured in human GH-producing pituitary tumor cells, by means of the nystatin-perforated whole cell clamp technique and Fura-2 AM.	Fura-2	256-262	somatostatin	Homo sapiens	95-107
1388192-2	1992	Co-cross-linking of CD4 and Fc gamma R by an anti-CD4 mAb (MAX.16H5) and by excess of goat anti-mouse Ig induced a biphasic increase of the free cytosolic Ca(2+)-concentration ([Ca2+]i) in the human monocytoid cell line U937 as measured by FURA-2 fluorescence.	Fura-2	240-246	CD4 molecule	Homo sapiens	20-23
1388192-2	1992	Co-cross-linking of CD4 and Fc gamma R by an anti-CD4 mAb (MAX.16H5) and by excess of goat anti-mouse Ig induced a biphasic increase of the free cytosolic Ca(2+)-concentration ([Ca2+]i) in the human monocytoid cell line U937 as measured by FURA-2 fluorescence.	Fura-2	240-246	CD4 molecule	Homo sapiens	50-53
1418854-7	1992	AII produced a concentration-related increase in calcium mobilization (fura-2 fluorescence) which was blocked by losartan (IC50 = 8.4 x 10(-8) mol/L) but not by PD123177 (10(-6) mol/L).	Fura-2	71-77	angiotensinogen	Rattus norvegicus	0-3
1393572-4	1992	Using Fura-2 loaded cells and dynamic ratio imaging, we have determined that arginine vasopressin (AVP) or V1- but not V2-receptor agonists, mobilise pituicyte intracellular Ca2+ ([Ca2+]i) in the absence of extracellular Ca2+.	Fura-2	6-12	arginine vasopressin	Rattus norvegicus	77-109
1527727-3	1992	Both thrombin and U46619 increased intracellular free calcium in fura-2 loaded, serum-deprived CHRF-288-11 cells with EC50 values of 0.07 +/- 0.01 U/ml and 194 +/- 27 nM, respectively (n = 4 each).	Fura-2	65-71	coagulation factor II, thrombin	Homo sapiens	5-13
1324922-1	1992	The regulation of extracellular Ca2+ entry into fura-2-loaded human platelets was examined following stimulation with thrombin.	Fura-2	48-54	coagulation factor II, thrombin	Homo sapiens	118-126
1505465-2	1992	[Ca2+]i was measured in cells loaded with the Ca(2+)-responsive fluorescent dye fura-2.	Fura-2	80-86	carbonic anhydrase 2	Gallus gallus	1-4
1328896-3	1992	Changes in intracellular Ca2+ of single cells, measured using the fluorescent indicator dye fura-2, indicated that bradykinin produced a rapid, but transient, increase in intracellular calcium.	Fura-2	92-98	kininogen 1	Homo sapiens	115-125
1380304-1	1992	In individual fura-2 loaded cells of rat pancreatic acini endothelin-1 (ET-1) (10-50 nM) induced sustained oscillations in [Ca2+]i.	Fura-2	14-20	endothelin 1	Rattus norvegicus	58-70
1380304-1	1992	In individual fura-2 loaded cells of rat pancreatic acini endothelin-1 (ET-1) (10-50 nM) induced sustained oscillations in [Ca2+]i.	Fura-2	14-20	endothelin 1	Rattus norvegicus	72-76
1514577-1	1992	In rat pancreatic and submandibular gland acini during exposure to carbachol, changes in the fluorescence emission intensity ratio (R) of acini loaded with mag-fura-2 resemble changes in cytosolic Ca2+ concentration (Ca2+i) in acini loaded with fura-2.	Fura-2	160-166	carbonic anhydrase 2	Rattus norvegicus	217-220
1330055-2	1992	BK, but not IL-1 beta, caused a rapid, transient rise of intracellular Ca2+ concentration ([Ca2+]i), as assessed by recordings of fura-2 fluorescence in monolayers of prelabelled gingival fibroblasts.	Fura-2	130-136	kininogen 1	Homo sapiens	0-2
1514647-5	1992	Stimulation of fura-2-loaded BPAEC with 10(-7) M alpha-thrombin produced a typical biphasic rise in [Ca2+]i.	Fura-2	15-21	coagulation factor II, thrombin	Bos taurus	55-63
1350327-6	1992	To study the mechanism that govern the spread of spikes in the dendrites of hippocampal pyramidal cells, we imaged Ca2+ entry with Fura-2 (ref.	Fura-2	131-137	carbonic anhydrase 2	Homo sapiens	115-118
1322351-0	1992	Epidermal growth factor induces dose-dependent calcium oscillations in single fura-2-loaded hepatocytes.	Fura-2	78-84	epidermal growth factor	Homo sapiens	0-23
1322351-1	1992	Digital imaging fluorescence microscopy has been used to investigate epidermal growth factor-induced calcium responses of fura-2-loaded hepatocytes in primary culture at the single-cell level.	Fura-2	122-128	epidermal growth factor	Homo sapiens	69-92
1321817-1	1992	Cai in cultured human sweat gland epithelial monolayers was measured using Fura-2 fluorescence.	Fura-2	75-81	carbonic anhydrase 1	Homo sapiens	0-3
1323639-4	1992	ET-1 dose-dependently (1-100 nmol/l) induced rapid and transient increases in [Ca2+]i in fura-2-labelled cells.	Fura-2	89-95	endothelin 1	Homo sapiens	0-4
1616943-0	1992	Cytosolic calcium oscillations induced by hepatocyte growth factor (HGF) in single fura-2-loaded cultured hepatocytes: effects of extracellular calcium and protein kinase C. Hepatocyte growth factor (HGF) induced the periodic fluctuations of cytosolic calcium concentration ([Ca2+]i) in primary cultured rat hepatocytes, which were dependent on extracellular calcium.	Fura-2	83-89	hepatocyte growth factor	Rattus norvegicus	42-66
1616943-0	1992	Cytosolic calcium oscillations induced by hepatocyte growth factor (HGF) in single fura-2-loaded cultured hepatocytes: effects of extracellular calcium and protein kinase C. Hepatocyte growth factor (HGF) induced the periodic fluctuations of cytosolic calcium concentration ([Ca2+]i) in primary cultured rat hepatocytes, which were dependent on extracellular calcium.	Fura-2	83-89	hepatocyte growth factor	Rattus norvegicus	68-71
1616943-0	1992	Cytosolic calcium oscillations induced by hepatocyte growth factor (HGF) in single fura-2-loaded cultured hepatocytes: effects of extracellular calcium and protein kinase C. Hepatocyte growth factor (HGF) induced the periodic fluctuations of cytosolic calcium concentration ([Ca2+]i) in primary cultured rat hepatocytes, which were dependent on extracellular calcium.	Fura-2	83-89	hepatocyte growth factor	Rattus norvegicus	174-198
1590371-7	1992	Exposure of fura-2-loaded SqCC cells to PTHRP and PTH resulted in equivalent, dose-dependent transient increases in intracellular calcium [half-maximal effective concentration (EC50) = 0.08 nM].	Fura-2	12-18	parathyroid hormone	Homo sapiens	40-43
1569926-1	1992	Human erythroleukemic (HEL) cells, loaded with fura-2, respond to neuropeptide Y (NPY) with a fast and transient increase in intracellular calcium.	Fura-2	47-53	neuropeptide Y	Homo sapiens	66-80
1590371-7	1992	Exposure of fura-2-loaded SqCC cells to PTHRP and PTH resulted in equivalent, dose-dependent transient increases in intracellular calcium [half-maximal effective concentration (EC50) = 0.08 nM].	Fura-2	12-18	parathyroid hormone like hormone	Homo sapiens	40-45
1533221-2	1992	Addition of vasopressin caused a second, rapid quench of cellular fura-2, whereas the addition of thapsigargin had no effect.	Fura-2	66-72	arginine vasopressin	Homo sapiens	12-23
1533221-4	1992	When Mn2+ was removed from the medium of fura-2/AM-loaded cells after preincubation with Mn2+, vasopressin still caused quench of cellular fura-2.	Fura-2	41-47	arginine vasopressin	Homo sapiens	95-106
1533221-4	1992	When Mn2+ was removed from the medium of fura-2/AM-loaded cells after preincubation with Mn2+, vasopressin still caused quench of cellular fura-2.	Fura-2	139-145	arginine vasopressin	Homo sapiens	95-106
1560205-4	1992	In the present work we have confirmed the above hypothesis by measuring the [Ca2+]i of fura-2- or aequorin-labeled YAC-1 cells treated with cytolysin and low Na+ medium or amiloride analogs.	Fura-2	87-93	perforin 1 (pore forming protein)	Mus musculus	140-149
1575681-0	1992	Spiking in cytosolic calcium concentration in single fibrinogen-bound fura-2-loaded human platelets.	Fura-2	70-76	fibrinogen beta chain	Homo sapiens	53-63
1575681-1	1992	Fura-2-loaded human platelets were immobilized on a fibrinogen-coated surface and the cytosolic free calcium concentration ([Ca2+]i) was measured in single platelets by low-light-level video-ratio image-processing of the optical probe signal.	Fura-2	0-6	fibrinogen beta chain	Homo sapiens	52-62
1592099-1	1992	The effect of thrombin-activated platelets and their release products on the intracellular free calcium concentration ([Ca2+]i) of human polymorphonuclear leukocytes (PMNs) was studied by loading PMNs with a fluorescent indicator of calcium, fura-2.	Fura-2	242-248	coagulation factor II, thrombin	Homo sapiens	14-22
1569926-1	1992	Human erythroleukemic (HEL) cells, loaded with fura-2, respond to neuropeptide Y (NPY) with a fast and transient increase in intracellular calcium.	Fura-2	47-53	neuropeptide Y	Homo sapiens	82-85
1535691-4	1992	The pharmacological profiles of the binding (determined with [3H]8-OH-DPAT) and the calcium response (measured using Fura-2) were clearly of the 5-HT1A type.	Fura-2	117-123	5-hydroxytryptamine receptor 1A	Homo sapiens	145-151
1544231-6	1992	Furthermore, apoptosis could be induced in HL-60 cells under conditions of vastly reduced [Ca2+]i achieved by loading these cells with fura-2 in the presence of EGTA.	Fura-2	135-141	carbonic anhydrase 2	Homo sapiens	91-94
1641824-2	1992	We used the fluorescent Ca2+ indicator, fura2, to measure both shape change as cell size and intracellular free Ca2+ ([Ca2+]i), in cultured human umbilical-vein endothelial cells (HUVEC).	Fura-2	40-45	carbonic anhydrase 2	Homo sapiens	24-27
1641824-2	1992	We used the fluorescent Ca2+ indicator, fura2, to measure both shape change as cell size and intracellular free Ca2+ ([Ca2+]i), in cultured human umbilical-vein endothelial cells (HUVEC).	Fura-2	40-45	carbonic anhydrase 2	Homo sapiens	112-115
1641824-2	1992	We used the fluorescent Ca2+ indicator, fura2, to measure both shape change as cell size and intracellular free Ca2+ ([Ca2+]i), in cultured human umbilical-vein endothelial cells (HUVEC).	Fura-2	40-45	carbonic anhydrase 2	Homo sapiens	112-115
1593447-2	1992	Changes in intracellular ionized calcium [Ca2+]i induced by human growth hormone releasing factor (hGRF) were analysed by quantitative fluorescent microscopy using a dual-wavelength, ratiometric video imaging system and low light level charge-coupled device (CCD) camera visualizing Fura-2 in dispersed male rat anterior pituitary cells.	Fura-2	283-289	growth hormone releasing hormone	Homo sapiens	66-97
1737761-7	1992	Under the same conditions, in fura-2-loaded cells, thrombin-evoked Ca2+ entry (monitored by Mn2+ quench) was detectable after a delay of 1.4 s. This suggests that early thrombin-evoked Ca2+ entry occurs via small conductance channels, below the resolution of the patch clamp technique, or by an electroneutral pathway.	Fura-2	30-36	coagulation factor II, thrombin	Homo sapiens	51-59
1737761-7	1992	Under the same conditions, in fura-2-loaded cells, thrombin-evoked Ca2+ entry (monitored by Mn2+ quench) was detectable after a delay of 1.4 s. This suggests that early thrombin-evoked Ca2+ entry occurs via small conductance channels, below the resolution of the patch clamp technique, or by an electroneutral pathway.	Fura-2	30-36	coagulation factor II, thrombin	Homo sapiens	169-177
1593447-2	1992	Changes in intracellular ionized calcium [Ca2+]i induced by human growth hormone releasing factor (hGRF) were analysed by quantitative fluorescent microscopy using a dual-wavelength, ratiometric video imaging system and low light level charge-coupled device (CCD) camera visualizing Fura-2 in dispersed male rat anterior pituitary cells.	Fura-2	283-289	growth hormone releasing hormone	Homo sapiens	99-103
1620579-3	1992	In cells expressing the oncogene, but not in NIH fibroblasts not expressing the oncogene, bradykinin elicits calcium oscillations, which are detected by fura-2 fluorescence and amplified by a decrease of extracellular sodium activity.	Fura-2	153-159	kininogen 1	Homo sapiens	90-100
1802362-7	1991	In the study with fura 2 excited at 340 nm, endothelin-1 abolished, from the tissue, the fluorescence signals that were coupled with spontaneous contraction.	Fura-2	18-24	endothelin-1	Cavia porcellus	44-56
1371475-1	1992	We report in this paper using measurement of intracellular free Ca2+ with fura-2, that flufenamic acid and several related blockers of the 25 pS Ca(2+)-activated non-selective cation channel cause release of Ca2+ from an intracellular store other than the endoplasmic reticulum, possibly from mitochondria.	Fura-2	74-80	carbonic anhydrase 2	Homo sapiens	64-67
1371475-1	1992	We report in this paper using measurement of intracellular free Ca2+ with fura-2, that flufenamic acid and several related blockers of the 25 pS Ca(2+)-activated non-selective cation channel cause release of Ca2+ from an intracellular store other than the endoplasmic reticulum, possibly from mitochondria.	Fura-2	74-80	carbonic anhydrase 2	Homo sapiens	208-211
1309423-6	1992	Finally, thrombin stimulated an increase in cytosolic ionized calcium concentration ([Ca2+]i) in fura2-loaded platelets that was abolished by concentrations of EC which also blocked thrombin-induced aggregation.	Fura-2	97-102	coagulation factor II, thrombin	Homo sapiens	9-17
1309423-6	1992	Finally, thrombin stimulated an increase in cytosolic ionized calcium concentration ([Ca2+]i) in fura2-loaded platelets that was abolished by concentrations of EC which also blocked thrombin-induced aggregation.	Fura-2	97-102	coagulation factor II, thrombin	Homo sapiens	182-190
1333253-2	1992	With PAF, fMLP and LTB4 as agonists the Ca-transients measured with Fura-2 showed the same height of the intracellular Ca-signal but an external Ca-component in decreasing magnitude.	Fura-2	68-74	PCNA clamp associated factor	Homo sapiens	5-8
1333253-2	1992	With PAF, fMLP and LTB4 as agonists the Ca-transients measured with Fura-2 showed the same height of the intracellular Ca-signal but an external Ca-component in decreasing magnitude.	Fura-2	68-74	formyl peptide receptor 1	Homo sapiens	10-14
1351266-1	1992	Spatiotemporal changes of the intracellular calcium ion (Ca2+) were recorded by digital ratio imaging of fura-2 in pyramidal neurons acutely isolated from the adult guinea-pig hippocampus.	Fura-2	105-111	LOW QUALITY PROTEIN: carbonic anhydrase 2	Cavia porcellus	57-60
1329268-2	1992	Both AII and AVP specifically induce a transient increases in cytosolic free calcium independent of extracellular calcium or calcium channels activated by high potassium depolarization in VSM cells loaded with Fura-2.	Fura-2	210-216	arginine vasopressin	Rattus norvegicus	13-16
1329268-2	1992	Both AII and AVP specifically induce a transient increases in cytosolic free calcium independent of extracellular calcium or calcium channels activated by high potassium depolarization in VSM cells loaded with Fura-2.	Fura-2	210-216	angiotensinogen	Rattus norvegicus	5-8
1939188-0	1991	Oscillations of intracellular calcium induced by vasopressin in individual fura-2-loaded mesangial cells.	Fura-2	75-81	arginine vasopressin	Rattus norvegicus	49-60
1663589-4	1991	Measurements of changes in cytosolic Cai in Fura-2 loaded PMN demonstrate a transient Cai increase after stimulation with 0.1 microM fMLP and an additional sustained elevation of Cai levels in the presence of thimerosal.	Fura-2	44-50	formyl peptide receptor 1	Homo sapiens	133-137
1719553-7	1991	When fura-2 was used as indicator for the cytosolic Ca2+, TRH induced a release from intracellular stores only once and had no effect on the intracellular Ca2+ concentration during further applications.	Fura-2	5-11	thyrotropin releasing hormone	Rattus norvegicus	58-61
1745976-4	1991	[Ca]i was assessed by use of fura-2, a calcium-sensitive fluorescent indicator.	Fura-2	29-35	carbonic anhydrase 1	Bos taurus	1-5
1930148-3	1991	In the current study we have used the fluorescent indicator fura-2 to monitor the level of cytosolic Ca2+ ([Ca2+]i) in human skin fibroblasts.	Fura-2	60-66	carbonic anhydrase 2	Homo sapiens	101-104
1953756-1	1991	The aim of the study was to investigate the relationship between thyrotropin-releasing hormone (TRH)-induced changes in intracellular free Ca2+ ([Ca2+]i), and influx of extracellular Ca2+ in Fura 2 loaded pituitary GH4C1 cells.	Fura-2	191-197	thyrotropin releasing hormone	Rattus norvegicus	65-94
1953756-1	1991	The aim of the study was to investigate the relationship between thyrotropin-releasing hormone (TRH)-induced changes in intracellular free Ca2+ ([Ca2+]i), and influx of extracellular Ca2+ in Fura 2 loaded pituitary GH4C1 cells.	Fura-2	191-197	thyrotropin releasing hormone	Rattus norvegicus	96-99
1953756-6	1991	In the presence of extracellular Mn2+, TRH stimulated quenching of Fura 2 fluorescence.	Fura-2	67-73	thyrotropin releasing hormone	Rattus norvegicus	39-42
1930148-3	1991	In the current study we have used the fluorescent indicator fura-2 to monitor the level of cytosolic Ca2+ ([Ca2+]i) in human skin fibroblasts.	Fura-2	60-66	carbonic anhydrase 2	Homo sapiens	108-111
1936540-4	1991	Resting intracellular free Ca2+ levels ([Ca2+]i), measured using Fura-2, was determined to be 60 +/- 14.3 nM (mean +/- SE).	Fura-2	65-71	carbonic anhydrase 2	Rattus norvegicus	27-30
1716460-5	1991	Exposure of fura-2-loaded acini to 10-1000 pM CCK-8 caused an immediate and dose-dependent increase in [Ca2+]i followed by a gradual decrease in [Ca2+]i.	Fura-2	12-18	cholecystokinin	Rattus norvegicus	46-49
1651654-9	1991	Measurements of cytosolic Ca2+ transients using fura-2 indicated that the ICa was responsible for triggering Ca2+ release from the sarcoplasmic reticulum.	Fura-2	48-54	LOW QUALITY PROTEIN: carbonic anhydrase 2	Cavia porcellus	26-29
1651654-9	1991	Measurements of cytosolic Ca2+ transients using fura-2 indicated that the ICa was responsible for triggering Ca2+ release from the sarcoplasmic reticulum.	Fura-2	48-54	LOW QUALITY PROTEIN: carbonic anhydrase 2	Cavia porcellus	109-112
1651495-2	1991	Measurements of intracellular Ca concentration [( Ca]i) using the fluorescent Ca indicator fura-2 showed that GABA potently inhibited Ca influx into the terminal elicited by high extracellular K concentration ([K]o).	Fura-2	91-97	carbonic anhydrase 1	Homo sapiens	50-54
1651669-1	1991	The Ca(2+)-mobilizing action of bradykinin (BK) was investigated in ciliated human nasal epithelial (HNE) cells utilizing fura-2 fluorescence and microspectrofluorimetry.	Fura-2	122-128	kininogen 1	Homo sapiens	32-42
1651669-1	1991	The Ca(2+)-mobilizing action of bradykinin (BK) was investigated in ciliated human nasal epithelial (HNE) cells utilizing fura-2 fluorescence and microspectrofluorimetry.	Fura-2	122-128	kininogen 1	Homo sapiens	44-46
1742799-3	1991	Using the fluorescent indicator Fura-2, the concentration of intracellular free calcium ([Ca2+]i) in resting state cells was calculated to be 116 +/- 18.5 nM.	Fura-2	32-38	carbonic anhydrase 2	Rattus norvegicus	90-93
1936540-4	1991	Resting intracellular free Ca2+ levels ([Ca2+]i), measured using Fura-2, was determined to be 60 +/- 14.3 nM (mean +/- SE).	Fura-2	65-71	carbonic anhydrase 2	Rattus norvegicus	41-44
1864358-1	1991	In fura-2-loaded parotid acinar cells, 50-200 microM sphingosine induced an increase in cytosolic Ca2+ ([Ca2+]i).	Fura-2	3-9	carbonic anhydrase 2	Rattus norvegicus	98-101
1650480-1	1991	Endothelin 1 causes a strong Ca2+ signal in C6 rat glioma cells as measured by fura-2 fluorescence.	Fura-2	79-85	endothelin 1	Rattus norvegicus	0-12
1864358-1	1991	In fura-2-loaded parotid acinar cells, 50-200 microM sphingosine induced an increase in cytosolic Ca2+ ([Ca2+]i).	Fura-2	3-9	carbonic anhydrase 2	Rattus norvegicus	105-108
1830482-6	1991	Concentration-dependent increases in intracellular free Ca2+ concentrations induced by I-BOP were observed in RASMC loaded with the calcium sensitive dye fura-2.	Fura-2	154-160	opsin 1, short wave sensitive	Rattus norvegicus	89-92
1945035-1	1991	Nerve growth factor (NGF) rapidly infused into the extracellular medium near cell bodies of cultured chick dorsal root ganglion neurons caused transient increases in intracellular calcium ions, which was detected by fura-2 fluorometry.	Fura-2	216-222	nerve growth factor	Gallus gallus	0-19
1945035-1	1991	Nerve growth factor (NGF) rapidly infused into the extracellular medium near cell bodies of cultured chick dorsal root ganglion neurons caused transient increases in intracellular calcium ions, which was detected by fura-2 fluorometry.	Fura-2	216-222	nerve growth factor	Gallus gallus	21-24
1653113-7	1991	Finally, experiments with Fura-2-loaded hepatocytes reveal that agents specifically releasing Ca2+ from non-mitochondrial stores (vasopressin and 2,5-di-tert-butyl-1-4-benzohydroquinone) are still able to increase the cytosolic Ca2+ concentration in FCCP-treated cells.	Fura-2	26-32	carbonic anhydrase 2	Rattus norvegicus	94-97
1905229-1	1991	Dual wavelength digital imaging microscopy to detect fura-2 has been employed to characterize in normal bovine PRL-secreting cells the effects of TRH and dopamine on the intracellular ionized calcium concentration [( Ca2+]i).	Fura-2	53-59	thyrotropin releasing hormone	Bos taurus	146-149
2065474-2	1991	Video imaging of single, fura2-loaded vascular smooth muscle cells was used to examine the spatial and temporal alterations in calcium, Ca2+, in response to low levels of vasoconstrictor stimuli.	Fura-2	25-30	carbonic anhydrase 2	Homo sapiens	136-139
1653853-11	1991	Calcium imaging experiments, using the Ca(2+)-sensitive probe, Fura-2, were performed to estimate the accumulation of Ca2+ in the presence of 10 mM-intracellular BAPTA.	Fura-2	63-69	LOW QUALITY PROTEIN: carbonic anhydrase 2	Cavia porcellus	118-121
1850329-12	1991	Using the fluorescent calcium indicator fura-2, we demonstrated that thrombin increased the beating rate, diastolic calcium, and peak systolic calcium of spontaneously contracting cultured ventricular myocytes.	Fura-2	40-46	coagulation factor II	Rattus norvegicus	69-77
1649127-1	1991	We have used single-cell ratio imaging of Fura-2 loaded neutrophils to visualize release of cytosolic Ca2+ from an intracellular store in order to determine the location of this store and the relationship of release from it to oxidase activation.	Fura-2	42-48	carbonic anhydrase 2	Homo sapiens	102-105
2026609-9	1991	The LHRH threshold and the quantal secretion process of metestrous gonadotropes was further studied by measuring cytosolic Ca2+ using fura-2 and digital imaging microscopy.	Fura-2	134-140	gonadotropin releasing hormone 1	Homo sapiens	4-8
1881195-3	1991	Application of the method to mossy fiber tracts in hippocampal region CA3 and parallel fiber tracts in cerebellum resulted in distant presynaptic terminals well labelled with fura-2 without concomitant postsynaptic labelling, allowing optical measurements of calcium concentration in individual presynaptic terminals.	Fura-2	175-181	carbonic anhydrase 3	Homo sapiens	70-73
2022753-6	1991	ET-1 dose-dependently induced increases in cytosolic free Ca2+ concentration in fura-2-loaded tumor cells, whose effect was completely abolished by chelating extracellular Ca2+ or by Ca(2+)-channel blocker.	Fura-2	80-86	endothelin 1	Homo sapiens	0-4
1648132-11	1991	Video imaging of single-cell Fura-2 fluorescence from both intact cells and patch-clamped cells showed temporal correlation of the K+ current modulation and the Ca2+ transients in response to bradykinin stimulation.	Fura-2	29-35	kininogen 1	Homo sapiens	192-202
1677314-1	1991	An increase in intracellular calcium concentration stimulated by anti-CD2 or CD3 antibodies has been measured with Fura-2 in P28 cells, a human CD4+ T cell clone.	Fura-2	115-121	CD2 molecule	Homo sapiens	70-73
1900792-9	1991	In single parietal cells double-labeled with fura-2 and BCECF, a temporal decrease in pHi preceded the rise in Cai after stimulation with nigericin.	Fura-2	45-51	glucose-6-phosphate isomerase	Homo sapiens	86-89
1881195-4	1991	Application of the method to CA1 pyramidal cells produced intracellular loading of apical dendrites with fura-2.	Fura-2	105-111	carbonic anhydrase 1	Homo sapiens	29-32
2002040-8	1991	As evident from fura 2 fluorescence, bradykinin leads to a transient increase of intracellular calcium both in the presence and absence of extracellular calcium.	Fura-2	16-22	kininogen 1	Homo sapiens	37-47
2067597-2	1991	In this paper, changes in intracellular Ca2+ concentration ([Ca2+]1) in response to CCK peptides were measured in single cultured rat striatal neuron by fura-2 fluorometry.	Fura-2	153-159	cholecystokinin	Rattus norvegicus	84-87
1900287-5	1991	A similar effect of thrombin was seen on video imaging of Fura-2-loaded cell monolayers.	Fura-2	58-64	coagulation factor II, thrombin	Homo sapiens	20-28
1900390-9	1991	Measurements of intracellular Ca2+ concentrations with fura-2 show that intracellular Ca2+ rises in hyposmotic solutions and that this rise does not occur in absence of extracellular Ca2+.	Fura-2	55-61	carbonic anhydrase 2	Oryctolagus cuniculus	30-33
1900390-9	1991	Measurements of intracellular Ca2+ concentrations with fura-2 show that intracellular Ca2+ rises in hyposmotic solutions and that this rise does not occur in absence of extracellular Ca2+.	Fura-2	55-61	carbonic anhydrase 2	Oryctolagus cuniculus	86-89
1900390-9	1991	Measurements of intracellular Ca2+ concentrations with fura-2 show that intracellular Ca2+ rises in hyposmotic solutions and that this rise does not occur in absence of extracellular Ca2+.	Fura-2	55-61	carbonic anhydrase 2	Oryctolagus cuniculus	86-89
2003581-12	1991	Simultaneous measurement of membrane K+ current and fura-2 fluorescence in the same cell demonstrated a correlation between the rise in [Ca2+]c and increase in K+ current.	Fura-2	52-58	carbonic anhydrase 2	Homo sapiens	137-140
1742874-5	1991	We used fura 2 (pentapotassium salt) to confirm that the ryanodine we used was capable of abolishing Ca2+ release from the sarcoplasmic reticulum during the period in which it was present.	Fura-2	8-14	LOW QUALITY PROTEIN: carbonic anhydrase 2	Cavia porcellus	101-104
1837489-1	1991	Using fura-2 loaded neural tumour cells, SK-N-SH, we demonstrate that receptor-mediated activation of phosphoinositide hydrolysis not only causes the release of Ca2+ from intracellular stores but also causes a concomitant influx of extracellular Ca2+.	Fura-2	6-12	carbonic anhydrase 2	Homo sapiens	161-164
1996698-8	1991	These findings, together with our previously described results using fura-2, suggest that oxidant stress initially inhibits bradykinin-stimulated Ca2+ influx and later inhibits stimulated Ca2+ efflux.	Fura-2	69-75	kininogen 1	Bos taurus	124-134
1705122-6	1991	Stimulation of fura-2 loaded cells with ET-1 produced an unusual timecourse of response which rose slowly to a maximum which was sustained.	Fura-2	15-21	endothelin 1	Bos taurus	40-44
2015414-1	1991	Using microfluorometric analysis in individual, fura-2 loaded cells, we found that thrombin (0.1-10 U/ml) caused a dose-dependent (EC50 approximately 0.5 U/ml), rapid (within seconds), transient increase in cytoplasmic Ca2+ in the osteoblastic cell line MC3T3-El.	Fura-2	48-54	coagulation factor II, thrombin	Homo sapiens	83-91
2059991-0	1991	Ca2+ oscillations induced by histamine H1 receptor stimulation in HeLa cells: Fura-2 and patch clamp analysis.	Fura-2	78-84	histamine receptor H1	Homo sapiens	29-50
1849803-1	1991	Through assessment of the changes in the intracellular free-calcium concentration ([Ca2+]i), which was measured using the calcium sensitive dye, fura-2, the character of the neurotensin (NT) binding sites which appeared transiently during the early ontogenetic stage in the rat cerebral cortex was analyzed in primary cultures of cerebral cortex cells from neonatal rats.	Fura-2	145-151	neurotensin	Rattus norvegicus	174-185
19215446-1	1991	Abstract The effect of thyrotrophin-releasing hormone (TRH) on intracellular free Ca(2+) concentration, [Ca(2+))i, was investigated with the fluorescent dye fura-2 in cell suspensions obtained from 13 human growth hormone-secreting adenomas and 6 adrenocorticotrophin-secreting adenomas.	Fura-2	157-163	thyrotropin releasing hormone	Homo sapiens	55-58
1725355-6	1991	ET-1 caused a prompt rise in the fura-2-associated fluorescence intensity in the individual A-10 cell and it fell to a lower plateau level that was still higher than the baseline value.	Fura-2	33-39	endothelin 1	Rattus norvegicus	0-4
1988097-1	1991	Digitized video-intensified fluorescence microscopy with the Ca2(+)-sensitive fluorescent dye fura-2 was used to measure cytosolic free Ca2+ [( Ca2+]f) in HT-29 human colon cancer cells.	Fura-2	94-100	carbonic anhydrase 2	Homo sapiens	61-64
1988097-1	1991	Digitized video-intensified fluorescence microscopy with the Ca2(+)-sensitive fluorescent dye fura-2 was used to measure cytosolic free Ca2+ [( Ca2+]f) in HT-29 human colon cancer cells.	Fura-2	94-100	carbonic anhydrase 2	Homo sapiens	136-139
1988097-1	1991	Digitized video-intensified fluorescence microscopy with the Ca2(+)-sensitive fluorescent dye fura-2 was used to measure cytosolic free Ca2+ [( Ca2+]f) in HT-29 human colon cancer cells.	Fura-2	94-100	carbonic anhydrase 2	Homo sapiens	136-139
1892734-1	1991	Stimulation of suspensions of fura-2-loaded human neutrophils with ATP resulted in an elevation in cytosolic free calcium concentration ([Ca2+]i) from a basal value of 0.1 microM to a transient peak of 1 microM.	Fura-2	30-36	carbonic anhydrase 2	Homo sapiens	138-141
1725305-1	1991	The present study was undertaken to examine the interaction between endothelin-1 (ET-1)-induced Ca2+ and Na+ kinetics in cultured rat vascular smooth muscle cells (VSMCs) by measuring cytosolic free Ca2+ ([Ca2+]i) and intracellular Na+ ([Na+]i) using intracellular fluorescent dyes (fura-2 and SBFI, respectively).	Fura-2	283-289	endothelin 1	Rattus norvegicus	68-80
1725305-1	1991	The present study was undertaken to examine the interaction between endothelin-1 (ET-1)-induced Ca2+ and Na+ kinetics in cultured rat vascular smooth muscle cells (VSMCs) by measuring cytosolic free Ca2+ ([Ca2+]i) and intracellular Na+ ([Na+]i) using intracellular fluorescent dyes (fura-2 and SBFI, respectively).	Fura-2	283-289	endothelin 1	Rattus norvegicus	82-86
1984207-1	1991	The changes in intracellular free calcium concentration ([Ca2+]i) induced by oxytocin in single cells of cultured human puerperal myometrium were measured with the calcium-sensitive fluorescent dye fura 2 in a digital imaging fluorescence microscopic system.	Fura-2	198-204	oxytocin/neurophysin I prepropeptide	Homo sapiens	77-85
1942669-1	1991	We describe a new system to measure changes in cytosolic free Ca concentration ([Ca2+]i) and pH (pHi) simultaneously using the dyes fura-2 and BCECF, and a random access camera with computer-operated synchronously-changing excitation and emission filters.	Fura-2	132-138	glucose-6-phosphate isomerase	Rattus norvegicus	97-100
1899461-1	1991	Confluent monolayers of human umbilical vein endothelial cells subcultured on glass coverslips were loaded with the fluorescent Ca2+ indicator, fura-2.	Fura-2	144-150	carbonic anhydrase 2	Homo sapiens	128-131
20504745-3	1991	A saturating concentration of ET-2 (100 nM) increased intracellular free calcium (measured by Fura-2 fluorescence) from a resting level of ?	Fura-2	94-100	endothelin 2	Mus musculus	30-34
1725410-5	1991	By fura-2 fluorescence method, transient increase and oscillation of intracellular Ca2+ level were observed in the cultured myofibroblasts following the application of ET-1 or endothelin-3 (ET-3).	Fura-2	3-9	endothelin 1	Rattus norvegicus	168-172
1725410-5	1991	By fura-2 fluorescence method, transient increase and oscillation of intracellular Ca2+ level were observed in the cultured myofibroblasts following the application of ET-1 or endothelin-3 (ET-3).	Fura-2	3-9	endothelin 3	Rattus norvegicus	176-188
1725410-5	1991	By fura-2 fluorescence method, transient increase and oscillation of intracellular Ca2+ level were observed in the cultured myofibroblasts following the application of ET-1 or endothelin-3 (ET-3).	Fura-2	3-9	endothelin 3	Rattus norvegicus	190-194
2260972-6	1990	The present study was undertaken to determine the effect of HgCl2 on ANG II-induced [Ca++]i increase using Fura-2.	Fura-2	107-113	angiotensinogen	Rattus norvegicus	69-75
2260994-5	1990	Pretreatment with the phospholipase A2 inhibitor dexamethasone potently inhibited both ET-1 and PAF-induced elevation of cytosolic free Ca2+ concentrations [( Ca2+]i) in fura-2-loaded VSMC.	Fura-2	170-176	phospholipase A2 group IB	Rattus norvegicus	22-38
2260994-5	1990	Pretreatment with the phospholipase A2 inhibitor dexamethasone potently inhibited both ET-1 and PAF-induced elevation of cytosolic free Ca2+ concentrations [( Ca2+]i) in fura-2-loaded VSMC.	Fura-2	170-176	endothelin 1	Rattus norvegicus	87-91
2175792-5	1990	Octimibate also inhibited the rise in intracellular Ca++ caused by thrombin, as measured using Fura-2-loaded platelets, which is consistent with octimibate"s ability to elevate platelet cAMP levels.	Fura-2	95-101	coagulation factor II, thrombin	Homo sapiens	67-75
2268367-4	1990	We observed in fura-2 loaded cells that pretreatment with manoalide blocked fMLP-induced increases in cytosolic free-calcium (IC50 approximately 0.15 microM).	Fura-2	15-21	formyl peptide receptor 1	Homo sapiens	76-80
2171931-7	1990	When the effect of erythropoietin and Me2SO on the intracellular Ca2+ level was examined using fura 2, no acute change was observed.	Fura-2	95-101	erythropoietin	Mus musculus	19-33
1701016-9	1990	Ca2+ mobilization, measured by fura-2 fluorescence, was coincident with the increase in tyrosine phosphorylation in response to angiotensin II or thapsigargin.	Fura-2	31-37	angiotensinogen	Rattus norvegicus	128-142
1704288-7	1990	Depolarization-induced [Ca2+]i transients assessed using fura-2 showed that the inhibitory effects of nitrendipine and NPY were additive.	Fura-2	57-63	neuropeptide Y	Rattus norvegicus	119-122
1963319-1	1990	Preincubation of Fura 2-loaded rat myometrial cells with H-8, an inhibitor of protein kinase A, for 1 h reversed the inhibitory effects of 8-(4-chlorophenylthio)-cAMP (CPTcAMP) on the oxytocin-stimulated increase in (Ca2+)i (intracellular free calcium), with an EC50 of 47 microM.	Fura-2	17-23	protein kinase cAMP-activated catalytic subunit alpha	Rattus norvegicus	78-103
2145278-1	1990	Stimulation of macrophages with platelet-activating factor (PAF) elicits an increase of intracellular calcium concentration, Ca2+i, which was monitored here at the single cell level with the calcium-sensitive dye Fura-2.	Fura-2	213-219	PCNA clamp associated factor	Homo sapiens	32-58
2145278-1	1990	Stimulation of macrophages with platelet-activating factor (PAF) elicits an increase of intracellular calcium concentration, Ca2+i, which was monitored here at the single cell level with the calcium-sensitive dye Fura-2.	Fura-2	213-219	PCNA clamp associated factor	Homo sapiens	60-63
2170382-3	1990	In the presence of extracellular Mn2+, the Ca2(+)-mobilizing hormone vasopressin produced a severalfold stimulation of the basal rate of fura-2 fluorescence quenching as a result of Mn2+ influx; this effect was blocked by the presence of Ni2+ in the incubation medium.	Fura-2	137-143	arginine vasopressin	Rattus norvegicus	69-80
2168414-3	1990	Although NaF induced the mobilization of Ca2+ from intracellular storage sites in fura2-loaded platelets, it failed to raise pHi as determined from the fluorescence of 2,7-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein-loaded platelets.	Fura-2	82-87	C-X-C motif chemokine ligand 8	Homo sapiens	9-12
2172374-2	1990	Endothelin-1 was found to increase the intracellular Ca2+ level, either in the presence or absence of extracellular Ca2+, in endothelial cells pretreated with antiserum by the fura-2 fluorescence technique.	Fura-2	176-182	endothelin 1	Homo sapiens	0-12
1963337-1	1990	Bradykinin triggered intracellular Ca mobilizations and ionic conductance changes were studied in the neuroblastoma x glioma hybrid cell line NG108-15 using Ca-sensitive fluorescent indicator fura-2 under patch pipette whole cell voltage clamp condition.	Fura-2	192-198	kininogen 1	Homo sapiens	0-10
2393384-1	1990	Cytoplasmic Ca2+ mobilization in human platelets triggered by a CD 9 monoclonal antibody, TP82, was monitored by the Ca2(+)-sensitive photoprotein, aequorin and Ca2(+)-sensitive fluorophores, fura 2 and quin 2.	Fura-2	192-198	CD9 molecule	Homo sapiens	64-68
2168700-4	1990	In the presence of Mn2+, an ion that quenches fura-2 fluorescence, fura-2-loaded platelets were stimulated with thrombin or ADP.	Fura-2	46-52	coagulation factor II, thrombin	Homo sapiens	112-120
2168700-4	1990	In the presence of Mn2+, an ion that quenches fura-2 fluorescence, fura-2-loaded platelets were stimulated with thrombin or ADP.	Fura-2	67-73	coagulation factor II, thrombin	Homo sapiens	112-120
2165364-2	1990	The unique properties of the CCD detector were applied to an analysis of cell shortening and the Ca2+ transient from fluorescence images of fura-2-loaded [corrected] cardiomyocytes.	Fura-2	140-146	carbonic anhydrase 2	Homo sapiens	97-100
2165364-8	1990	Raising the intracellular load of fura-2 had little effect on the rising phase of Ca2+ or the extent of shortening but extended the duration of the Ca2+ transient and contraction.	Fura-2	34-40	carbonic anhydrase 2	Homo sapiens	148-151
2162247-7	1990	Fura2 experiments also demonstrated that, in addition to omega CTx-sensitive calcium channels, SCC cell lines also expressed omega CTx-insensitive calcium channels, which were antagonized by nitrendipine and verapamil.	Fura-2	0-5	serpin family B member 3	Homo sapiens	95-98
2388255-9	1990	Because fura-2 was found to decrease its affinity for Ca as pH decreased below 6.7, corrections were applied to experiments in which large pHi changes were observed.	Fura-2	8-14	glucose-6-phosphate isomerase	Oryctolagus cuniculus	139-142
2161346-5	1990	Using the intracellular Ca2(+)-indicator fura-2, we observed that the addition of thrombin to the glioma cells generated a Ca2(+)-signal which was inhibited by pretreatment of the cells with hirudin or with 1 mM neomycin.	Fura-2	41-47	coagulation factor II	Rattus norvegicus	82-90
2113061-1	1990	Digital imaging fluorescence microscopy of fura-2-loaded hepatocytes in primary culture has been used to examine the changes of cytosolic free Ca2+ ([Ca2+]i) in response to receptor activation by alpha 1-adrenergic agonists and vasopressin at the subcellular level.	Fura-2	43-49	arginine vasopressin	Homo sapiens	228-239
2383431-4	1990	At the concentrations tested nitric oxide effectively blocked thrombin-stimulated mobilization of cytosolic-free calcium in Fura 2-loaded platelets.	Fura-2	124-130	coagulation factor II, thrombin	Homo sapiens	62-70
2161879-8	1990	Peak levels of intracellular Ca2+ ([Ca]i) at 5 to 10 s, and levels of [Ca]i 120 s after FMLP in Fura 2-loaded cells were significantly lower in cells treated with lidocaine, findings that could be explained by enhanced 45Ca2+ efflux from neutrophils.	Fura-2	96-102	formyl peptide receptor 1	Homo sapiens	88-92
1973064-2	1990	[Ca2+]i was measured with Fura-2 using the ratiometric method.	Fura-2	26-32	carbonic anhydrase 2	Homo sapiens	1-4
2338678-8	1990	Thrombin 0.5 U/ml added to platelets in Ca-free media caused a multiphasic rise in (Ca)i with Fura 2.	Fura-2	94-100	coagulation factor II, thrombin	Homo sapiens	0-8
2318379-0	1990	Effect of buffer systems and pHi on the measurement of [Ca2+]i with fura 2.	Fura-2	68-74	glucose-6-phosphate isomerase	Rattus norvegicus	29-32
2318379-7	1990	It was found that measurements of [Ca2+]i with fura 2 were influenced by shifts in pHi that occur when cells are incubated in either HEPES-buffered or CO2/HCO3- media of differing pHo values.	Fura-2	47-53	glucose-6-phosphate isomerase	Rattus norvegicus	83-86
2158310-1	1990	In fura-2-loaded A10 vascular smooth-muscle cells, 1 nM-vasopressin and 200 nM-endothelin evoked a rapid transient rise in intracellular free Ca2+ concentration [( Ca2+]i), which was then followed by a maintained elevation of [Ca2+]i.	Fura-2	3-9	immunoglobulin kappa variable 6D-21 (non-functional)	Homo sapiens	17-20
2158310-1	1990	In fura-2-loaded A10 vascular smooth-muscle cells, 1 nM-vasopressin and 200 nM-endothelin evoked a rapid transient rise in intracellular free Ca2+ concentration [( Ca2+]i), which was then followed by a maintained elevation of [Ca2+]i.	Fura-2	3-9	arginine vasopressin	Homo sapiens	56-67
2158310-3	1990	When extracellular Ca2+ was replaced with 1 mM-Mn2+ (Mn2+ quenches fura-2 fluorescence), both endothelin and vasopressin evoked an Mn2+ quench of the fluorescence from the intracellularly trapped fura-2, even in the presence of 5 microM-nifedipine.	Fura-2	67-73	arginine vasopressin	Homo sapiens	109-120
2158310-3	1990	When extracellular Ca2+ was replaced with 1 mM-Mn2+ (Mn2+ quenches fura-2 fluorescence), both endothelin and vasopressin evoked an Mn2+ quench of the fluorescence from the intracellularly trapped fura-2, even in the presence of 5 microM-nifedipine.	Fura-2	196-202	arginine vasopressin	Homo sapiens	109-120
2155611-1	1990	Vasoactive intestinal contractor peptide (VIC), a novel member of the endothelin family, stimulated a rapid increase in the intracellular Ca2+ concentration in fura-2-loaded Swiss 3T3 cells.	Fura-2	160-166	endothelin 2	Mus musculus	0-40
2306515-1	1990	We found that a monoclonal antibody (MoAb) to CD9 antigen, PMA2, induced a rise in cytosolic free calcium concentration ([Ca2+]i) in fura-2-loaded platelets, and we examined whether this response was due to direct action of PMA2 on CD9 antigen.	Fura-2	133-139	CD9 molecule	Homo sapiens	46-49
2155611-1	1990	Vasoactive intestinal contractor peptide (VIC), a novel member of the endothelin family, stimulated a rapid increase in the intracellular Ca2+ concentration in fura-2-loaded Swiss 3T3 cells.	Fura-2	160-166	endothelin 2	Mus musculus	42-45
2155611-1	1990	Vasoactive intestinal contractor peptide (VIC), a novel member of the endothelin family, stimulated a rapid increase in the intracellular Ca2+ concentration in fura-2-loaded Swiss 3T3 cells.	Fura-2	160-166	endothelin 1	Mus musculus	70-80
2295684-7	1990	We have used microspectrofluorometry to measure [Ca2+]i in single fura-2-loaded human neutrophils as they moved on poly-D-lysine-coated glass in the presence of serum.	Fura-2	66-72	carbonic anhydrase 2	Homo sapiens	49-52
2154452-1	1990	The effects of membrane potential on resting and bradykinin-stimulated changes in [Ca2+]i were measured in fura-2 loaded cultured endothelial cells from bovine atria by spectrofluorimetry.	Fura-2	107-113	kininogen 1	Bos taurus	49-59
2306207-0	1990	ADP evokes biphasic Ca2+ influx in fura-2-loaded human platelets.	Fura-2	35-41	carbonic anhydrase 2	Homo sapiens	20-23
2306207-2	1990	Stopped-flow fluorimetric studies at 37 degrees C have shown that ADP, at optimal concentrations, can evoke Ca2+ or Mn2+ influx in fura-2-loaded human platelets without measurable delay.	Fura-2	131-137	carbonic anhydrase 2	Homo sapiens	108-111
1688510-1	1990	The tachykinin family of neuropeptides, including substance P and neurokinins A and B, induce a transient increase in intracellular free calcium concentration in human small cell lung carcinoma (SCLC) cells, as measured with a calcium indicator fura-2.	Fura-2	245-251	tachykinin precursor 1	Homo sapiens	50-61
1688510-1	1990	The tachykinin family of neuropeptides, including substance P and neurokinins A and B, induce a transient increase in intracellular free calcium concentration in human small cell lung carcinoma (SCLC) cells, as measured with a calcium indicator fura-2.	Fura-2	245-251	tachykinin precursor 1	Homo sapiens	66-85
2328733-1	1990	A digital imaging microscope and fluorescent Ca(2+)-sensitive probe (Fura 2) were used to study the spatial location and time course of increases in free intracellular calcium (Cai) induced by platelet-derived growth factor (PDGF).	Fura-2	69-75	protein disulfide isomerase associated 4	Mus musculus	168-175
2328733-1	1990	A digital imaging microscope and fluorescent Ca(2+)-sensitive probe (Fura 2) were used to study the spatial location and time course of increases in free intracellular calcium (Cai) induced by platelet-derived growth factor (PDGF).	Fura-2	69-75	protein disulfide isomerase associated 4	Mus musculus	177-180
2154211-4	1990	In addition, PAF from 10(-6)-10(-8)M when added to Fura-2 containing B cells induced a rapid and significant rise of calcium within the cell, with lyso-PAF having no effect.	Fura-2	51-57	PCNA clamp associated factor	Homo sapiens	13-16
2153577-3	1990	Both NPY and the specific Y1 receptor ligand, [Leu31,Pro34]-NPY, caused a rapid and transient increase in the concentration of free calcium in the cytoplasm as measured by the fluorescent probe, Fura-2.	Fura-2	195-201	neuropeptide Y	Homo sapiens	5-8
2153577-3	1990	Both NPY and the specific Y1 receptor ligand, [Leu31,Pro34]-NPY, caused a rapid and transient increase in the concentration of free calcium in the cytoplasm as measured by the fluorescent probe, Fura-2.	Fura-2	195-201	neuropeptide Y	Homo sapiens	60-63
2294097-1	1990	The effects of oxidant stress and inhibition of glutathione reductase on the bradykinin-stimulated changes in cytosolic free Ca2+ concentration ([Ca2+]i) of calf pulmonary artery endothelial cells were determined using the intracellular fluorescent probe, fura-2.	Fura-2	256-262	kininogen 1	Bos taurus	77-87
2298824-8	1990	The effect of EGF on the intracellular mobilization of Ca2+ and the formation of inositol phosphates was studied by using the calcium-sensitive fluorescent indicator fura 2 and [3H]inositol.	Fura-2	166-172	epidermal growth factor	Mus musculus	14-17
33788969-5	2021	MrgprX1 receptor signaling was monitored by Fura-2-based Ca2+ imaging using PNEs and membrane potential responses were measured using FluoVoltTM .	Fura-2	44-50	MAS related GPR family member X1	Homo sapiens	0-7
1330027-12	1992	In two experiments involving the injection of larger amounts of fura-2, reductions in SR Ca2+ release were observed; however, we were unable to decide whether these reductions were due to [fura-2T] or to some nonspecific effect of the injection itself.	Fura-2	64-70	carbonic anhydrase 2	Homo sapiens	89-92
7714589-2	1995	Individual immortalized gonadotropin-releasing hormone (GnRH)-secreting hypothalamic (GT1-7) neurons in semiconfluent cultures showed spontaneous oscillations in intracellular Ca2+ concentration ([Ca2+]i) as measured by video fluorescence microscopy and fura-2.	Fura-2	254-260	gonadotropin releasing hormone 1	Homo sapiens	24-54
7714589-2	1995	Individual immortalized gonadotropin-releasing hormone (GnRH)-secreting hypothalamic (GT1-7) neurons in semiconfluent cultures showed spontaneous oscillations in intracellular Ca2+ concentration ([Ca2+]i) as measured by video fluorescence microscopy and fura-2.	Fura-2	254-260	beta-1,4-galactosyltransferase 1	Homo sapiens	86-91
1330027-1	1992	Experiments were carried out to test the hypothesis that mM concentrations of fura-2, a high-affinity Ca2+ buffer, inhibit the release of Ca2+ from the sarcoplasmic reticulum (SR) of skeletal muscle fibers.	Fura-2	78-84	carbonic anhydrase 2	Homo sapiens	102-105
1330027-1	1992	Experiments were carried out to test the hypothesis that mM concentrations of fura-2, a high-affinity Ca2+ buffer, inhibit the release of Ca2+ from the sarcoplasmic reticulum (SR) of skeletal muscle fibers.	Fura-2	78-84	carbonic anhydrase 2	Homo sapiens	138-141
1330027-4	1992	At [fura-2T] = 2-3 mM, the amplitude and half-width of delta [Ca2+] were reduced to approximately 25% of the values measured at [fura-2T] less than 0.15 mM, whereas the amount and rate of SR Ca2+ release were enhanced by approximately 50% (n = 5; 16 degrees C).	Fura-2	4-10	carbonic anhydrase 2	Homo sapiens	62-65
1330027-4	1992	At [fura-2T] = 2-3 mM, the amplitude and half-width of delta [Ca2+] were reduced to approximately 25% of the values measured at [fura-2T] less than 0.15 mM, whereas the amount and rate of SR Ca2+ release were enhanced by approximately 50% (n = 5; 16 degrees C).	Fura-2	4-10	carbonic anhydrase 2	Homo sapiens	191-194
1330027-4	1992	At [fura-2T] = 2-3 mM, the amplitude and half-width of delta [Ca2+] were reduced to approximately 25% of the values measured at [fura-2T] less than 0.15 mM, whereas the amount and rate of SR Ca2+ release were enhanced by approximately 50% (n = 5; 16 degrees C).	Fura-2	129-135	carbonic anhydrase 2	Homo sapiens	62-65
34607973-11	2021	We evaluated the accumulation of VEGF by fura-2/AM fluorescence.	Fura-2	41-47	vascular endothelial growth factor A	Mus musculus	33-37
34950652-5	2021	The effects of hypoxia and p65 expression on cell proliferation, invasion, apoptosis, (Ca2+)i, PPARgamma, and TRPC1/6 expression were determined using Cell Counting Kit-8 (CCK-8), Transwell, Annexin V/PI, Fura-2/AM, and western blotting, respectively.	Fura-2	205-211	RELA proto-oncogene, NF-kB subunit	Homo sapiens	27-30
34737238-6	2021	Using Fura-2 based Ca2+ imaging, we observed reproducible intracellular Ca2+ responses induced by Pam3CSK4 and Pam2CSK4 (TLR2-specific agonists) in TG neurons of adult wild-type (WT) mice.	Fura-2	6-12	toll-like receptor 2	Mus musculus	121-125
2686633-4	1989	Intracellular calcium concentration [( Ca]i) was measured in single H7CM cells using the fluorescent calcium indicator fura-2.	Fura-2	119-125	carbonic anhydrase 1	Homo sapiens	39-43
35302175-4	2022	The effect of Sig-1R overexpression on cell proliferation detected by EdU staining, cell cycle progression by propidium iodide (PI), apoptosis by Annexin V-APC/PI, mitochondrial membrane potential by Mitolite Red and cytoplasmic Ca2+ levelsby Fura-2/AM in islet beta-cells were measured by flow cytometry.	Fura-2	243-249	sigma non-opioid intracellular receptor 1	Mus musculus	14-20
2558572-2	1989	ET specifically binds to a single class of high-affinity receptors in MC3T3-E1 cells and induces phospholipase C activation with the production of two second messengers, inositol trisphosphate and 1,2-diacylglycerol, and a biphasic increase in intracellular free Ca2+ concentration ([Ca2+]i), which consists of an initial transient increase and an ensuing sustained plateau, as measured with a fluorescent indicator, fura-2.	Fura-2	417-423	endothelin 1	Mus musculus	0-2
2531141-1	1989	Fura-2 fluorescence in single rat basophilic leukemia cells was monitored to study the rise in intracellular free ionized calcium ([Ca2+]i) produced by aggregation of immunoglobulin E receptors.	Fura-2	0-6	carbonic anhydrase 2	Rattus norvegicus	132-135
34995919-10	2022	However, STIM1 knocking down reduced NCX1 activity recorded by either patch-clamp electrophysiology or Fura-2 single-cell microfluorimetry.	Fura-2	103-109	stromal interaction molecule 1	Rattus norvegicus	9-14
2514601-2	1989	In this study, [Ca2+]i was determined with the fluorescence indicator fura-2 at the single-cell level with a fluorescent microscope-imaging system.	Fura-2	70-76	carbonic anhydrase 2	Homo sapiens	16-19
2808386-5	1989	IGF-I also increases [Ca2+]i, measured using fura-2, a fluorescent Ca2+ indicator; the IGF-I-induced [Ca2+]i response occurs immediately, reaches a maximum within 1 min, and then slowly declines.	Fura-2	45-51	insulin like growth factor 1	Homo sapiens	0-5
2684691-1	1989	Effects on [Ca2+]i levels of endothelin-l (ET) and vasoactive intestinal contractor peptide (VIC), which is a novel member of the endothelin family, were examined in fura 2-loaded neuroblastoma NG108-15 cells.	Fura-2	166-172	endothelin 2	Mus musculus	93-96
2808386-5	1989	IGF-I also increases [Ca2+]i, measured using fura-2, a fluorescent Ca2+ indicator; the IGF-I-induced [Ca2+]i response occurs immediately, reaches a maximum within 1 min, and then slowly declines.	Fura-2	45-51	insulin like growth factor 1	Homo sapiens	87-92
2777805-2	1989	We studied thrombin-induced changes in cytosolic calcium concentration (Ca2+i) using microfluorometry of fura-2-loaded single cells, cell topography (scanning electron microscopy), and cytoskeleton (rhodamine phalloidin) in endothelial cells.	Fura-2	105-111	coagulation factor II, thrombin	Homo sapiens	11-19
2573029-4	1989	(EMBO J., 8, 159-166, 1989), EGF (10(-9)-10(-8) M) was found to induce marked increases of both [Ca2+]i and plasma membrane potential (investigated by the fura-2 and bis-oxonol techniques) which, in their initial phase, were only marginally dependent on the presence of Ca2+ in the incubation medium.	Fura-2	155-161	epidermal growth factor	Homo sapiens	29-32
2793847-0	1989	Characterization of cytosolic calcium oscillations induced by phenylephrine and vasopressin in single fura-2-loaded hepatocytes.	Fura-2	102-108	arginine vasopressin	Homo sapiens	80-91
2782436-4	1989	The effect of membrane depolarization with high K+ buffer on the bradykinin-stimulated changes in cytosolic Ca2+ was determined using the fluorescent Ca2+ indicator, fura-2.	Fura-2	166-172	kininogen 1	Bos taurus	65-75
2508482-6	1989	Parathyroid hormone (PTH) secretion was inhibited by ATP gamma S but not by those nucleotides that were without effect on [Ca2+]i. Loading cells with 1,2-bis-(2-aminophenoxy)ethane-N,N,N",N"-tetraacetic acid and fura-2 blocked cytosolic Ca2+ transients elicited by ATP gamma S but did not block the inhibitory effects of ATP gamma S on PTH secretion.	Fura-2	212-218	parathyroid hormone	Bos taurus	0-19
2508482-6	1989	Parathyroid hormone (PTH) secretion was inhibited by ATP gamma S but not by those nucleotides that were without effect on [Ca2+]i. Loading cells with 1,2-bis-(2-aminophenoxy)ethane-N,N,N",N"-tetraacetic acid and fura-2 blocked cytosolic Ca2+ transients elicited by ATP gamma S but did not block the inhibitory effects of ATP gamma S on PTH secretion.	Fura-2	212-218	parathyroid hormone	Bos taurus	21-24
2546937-5	1989	Fura-2 fluorescence was used to compare the profiles for BK-stimulated changes in cytosolic Ca2+, Sr2+, and Ba2+ (Ca2+i, Ba2+i, and Sr2+i).	Fura-2	0-6	kininogen 1	Homo sapiens	57-59
2550000-1	1989	The ability of cholecystokinin (CCK) to elevate intracellular Ca2+ levels in small cell lung cancer cells was investigated using the fluorescent Ca2+ indicator Fura 2.	Fura-2	160-166	cholecystokinin	Homo sapiens	15-30
2550000-1	1989	The ability of cholecystokinin (CCK) to elevate intracellular Ca2+ levels in small cell lung cancer cells was investigated using the fluorescent Ca2+ indicator Fura 2.	Fura-2	160-166	cholecystokinin	Homo sapiens	32-35
2775282-5	1989	It is concluded that [Ca2+]i and pHi could be measured simultaneously in isolated myocyte using dual-loading of fura-2 and BCECF.	Fura-2	112-118	glucose-6-phosphate isomerase	Rattus norvegicus	33-36
2545786-3	1989	PAF was found to elicit an increase in the cytoplasmic level of free Ca2+ as monitored by fluorescent indicator fura 2.	Fura-2	112-118	PCNA clamp associated factor	Homo sapiens	0-3
2545786-8	1989	PAF raised the fluorescence of fura 2 at concentrations as low as 8 pM which reached a maximal level about 8 nM, whereas more than nM order concentrations of PAF was required for the detectable release of [3H]AA.	Fura-2	31-37	PCNA clamp associated factor	Homo sapiens	0-3
2545786-10	1989	However, the fura 2 response to PAF was not effectively inhibited by toxin treatment.	Fura-2	13-19	PCNA clamp associated factor	Homo sapiens	32-35
2503036-2	1989	Acetylcholine (ACh), tetragastrin (TG), histamine and forskolin induced a transitory increase of intracellular Ca2+ concentration, [Ca2+]i, measured in gastric glands loaded with Ca2+-sensitive dye fura-2, and provoked an acid secretory response evaluated with aminopyrine accumulation ratio (AP ratio).	Fura-2	198-204	carbonic anhydrase 2	Oryctolagus cuniculus	111-114
2764121-4	1989	Changes in pHi and Cai2+ were measured with the fluorescent probes, 2,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF) and fura-2.	Fura-2	124-130	glucose-6-phosphate isomerase	Rattus norvegicus	11-14
2688858-2	1989	The former compounds, especially inositol 1,4,5-triphosphate, are known to cause the release of calcium from intracellular stores, while diacylglycerol is a potent activator of protein kinase C. LHRH causes a rapid and transient increase in intracellular concentrations of free calcium ions, by approximately 4.5-fold, in the majority of granulosa cells as assessed by fura-2 microspectrofluorimetry.	Fura-2	369-375	gonadotropin releasing hormone 1	Rattus norvegicus	195-199
2548389-11	1989	The time course of the initial hyperpolarization was shown to be similar to the calcium transient observed in fura-2-loaded A10 cell suspensions after the application of AVP.	Fura-2	110-116	arginine vasopressin	Rattus norvegicus	170-173
2503036-2	1989	Acetylcholine (ACh), tetragastrin (TG), histamine and forskolin induced a transitory increase of intracellular Ca2+ concentration, [Ca2+]i, measured in gastric glands loaded with Ca2+-sensitive dye fura-2, and provoked an acid secretory response evaluated with aminopyrine accumulation ratio (AP ratio).	Fura-2	198-204	carbonic anhydrase 2	Oryctolagus cuniculus	132-135
2663854-5	1989	In fura-2-loaded parotid acinar cells, thapsigargin caused a sustained elevation of [Ca2+], but did not increase inositol phosphate formation.	Fura-2	3-9	carbonic anhydrase 2	Homo sapiens	85-88
2503036-2	1989	Acetylcholine (ACh), tetragastrin (TG), histamine and forskolin induced a transitory increase of intracellular Ca2+ concentration, [Ca2+]i, measured in gastric glands loaded with Ca2+-sensitive dye fura-2, and provoked an acid secretory response evaluated with aminopyrine accumulation ratio (AP ratio).	Fura-2	198-204	carbonic anhydrase 2	Oryctolagus cuniculus	132-135
2568157-1	1989	Changes of intracellular Ca2+ concentration [( Ca2+]i) in response to somatostatin were measured in cultured rat hippocampal neurons on a single cell basis by fura-2 fluorometry.	Fura-2	159-165	somatostatin	Rattus norvegicus	70-82
2575164-2	1989	Intracellular free calcium ([Ca2+]i) was monitored by means of Fura-2 fluorescence measurements in hippocampal cells in primary cultures from newborn rats.	Fura-2	63-69	carbonic anhydrase 2	Rattus norvegicus	29-32
2499358-8	1989	When the intracellular free Ca2+ concentration ([Ca2+]i) was measured using fura-2, the thrombin-induced [Ca2+]i increase was reduced in platelets which had been treated with a phospholipase A2 inhibitor, ONO-RS-082 (2-(p-amylcinnamoyl)amino-4-chlorobenzoic acid).	Fura-2	76-82	coagulation factor II, thrombin	Homo sapiens	88-96
2499358-8	1989	When the intracellular free Ca2+ concentration ([Ca2+]i) was measured using fura-2, the thrombin-induced [Ca2+]i increase was reduced in platelets which had been treated with a phospholipase A2 inhibitor, ONO-RS-082 (2-(p-amylcinnamoyl)amino-4-chlorobenzoic acid).	Fura-2	76-82	phospholipase A2 group IB	Homo sapiens	177-193
2519888-1	1989	Since one of the first measurable events that occurs as a consequence of receptor-mediated cell activation is an increase in the cytosolic free calcium concentration, the calcium-selective fluorescent indicator fura-2 was employed to monitor increases in cytosolic calcium following PAF-acether stimulation of human neutrophils and platelets.	Fura-2	211-217	PCNA clamp associated factor	Homo sapiens	283-286
2544447-1	1989	In single mouse macrophages stimulated by platelet-activating factor (PAF), the intracellular calcium concentration (Cai) monitored with fura-2 at room temperature presents a biphasic increase, including a transient and a more sustained component.	Fura-2	137-143	patchy fur	Mus musculus	42-68
2544447-1	1989	In single mouse macrophages stimulated by platelet-activating factor (PAF), the intracellular calcium concentration (Cai) monitored with fura-2 at room temperature presents a biphasic increase, including a transient and a more sustained component.	Fura-2	137-143	patchy fur	Mus musculus	70-73
2541621-2	1989	Arginine vasopressin (AVP, 100 nM) rapidly increased cytosolic calcium (Caf) measured with fura-2 from a basal level of 65 +/- 5 to 516 +/- 102 nM followed by a return to a plateau level of 128 +/- 18 nM.	Fura-2	91-97	vasopressin	Sus scrofa	9-20
2540185-10	1989	Fura-2-based microfluorimetry of single dorsal root ganglion neurons demonstrated that both BK and NPY increased cytoplasmic-free Ca2+ concentration and that both peptides could produce this effect in the same neuron.	Fura-2	0-6	neuropeptide Y	Rattus norvegicus	99-102
2730577-0	1989	Receptor-mediated calcium entry in fura-2-loaded human platelets stimulated with ADP and thrombin.	Fura-2	35-41	coagulation factor II, thrombin	Homo sapiens	89-97
2730577-2	1989	Previous studies of the early kinetics of rises in cytosolic free [Ca2+] in fura-2-loaded human platelets suggested that: (1) Ca2+ entry slightly preceded internal discharge with thrombin and other agonists known to promote inositol lipid hydrolysis; (2) with ADP, Ca2+ entry occurred without measurable delay and clearly preceded internal Ca2+ discharge.	Fura-2	76-82	coagulation factor II, thrombin	Homo sapiens	179-187
2524580-3	1989	We report that peptide chemoattractants such as N-formyl-methionyl-leucyl-phenylalanine (fMLP) and the complement fragment C5a in direct comparative studies induced lower amounts of initial Ca2+ mobilization in PMNs from JP patients than healthy controls, as monitored by intracellular fura-2 fluorescence.	Fura-2	286-292	formyl peptide receptor 1	Homo sapiens	89-93
2524580-3	1989	We report that peptide chemoattractants such as N-formyl-methionyl-leucyl-phenylalanine (fMLP) and the complement fragment C5a in direct comparative studies induced lower amounts of initial Ca2+ mobilization in PMNs from JP patients than healthy controls, as monitored by intracellular fura-2 fluorescence.	Fura-2	286-292	complement C5a receptor 1	Homo sapiens	123-126
2519888-5	1989	The ability of different compounds to antagonize the PAF-acether-induced increase in cytosolic calcium was investigated using quin2 and fura-2 as calcium indicators.	Fura-2	136-142	PCNA clamp associated factor	Homo sapiens	53-56
2912686-2	1989	In 18 out of 44 individual rat granulosa cells loaded with fura-2, Ang II caused a rapid and transient increase in intracellular free calcium ion concentration, [Ca2+]i.	Fura-2	59-65	angiotensinogen	Rattus norvegicus	67-73
2460452-6	1988	Accordingly, 1 nM IGF-I caused a gradual increase in cytoplasmic free calcium concentration measured by fura2.	Fura-2	104-109	insulin-like growth factor 1	Mus musculus	18-23
2537401-4	1989	In fura-2-loaded, differentiated U-937 cells, PAF induced immediate and concentration-dependent calcium mobilization [( Ca++]i) that was inhibited by CV3988, but not by calcium channel blockers.	Fura-2	3-9	PCNA clamp associated factor	Homo sapiens	46-49
2537401-4	1989	In fura-2-loaded, differentiated U-937 cells, PAF induced immediate and concentration-dependent calcium mobilization [( Ca++]i) that was inhibited by CV3988, but not by calcium channel blockers.	Fura-2	3-9	carbonic anhydrase 1	Homo sapiens	120-126
2909543-1	1989	Bradykinin-evoked rises in [Ca2+]i were measured in fura-2-loaded bovine pulmonary artery endothelial cell monolayers by dual wavelength excitation fluorimetry.	Fura-2	52-58	kininogen 1	Bos taurus	0-10
2731227-2	1989	When stimulated with the agonists, angiotensin II and arginine vasopressin, cells incubated with low concentrations of fura-2 or indo-1 (less than 1 microM) produced Ca2+ transients characterized by a small increase followed by a dramatic decrease in fluorescence below the original baseline.	Fura-2	119-125	angiotensinogen	Rattus norvegicus	35-49
2731227-2	1989	When stimulated with the agonists, angiotensin II and arginine vasopressin, cells incubated with low concentrations of fura-2 or indo-1 (less than 1 microM) produced Ca2+ transients characterized by a small increase followed by a dramatic decrease in fluorescence below the original baseline.	Fura-2	119-125	arginine vasopressin	Rattus norvegicus	63-74
2473295-4	1989	ET-1 dose-dependently induced increases in [Ca2+]i of both cells loaded with the fluorescent Ca2+ indicator fura-2.	Fura-2	108-114	endothelin 1	Rattus norvegicus	0-4
2473325-6	1989	ET-1 and EC-CM induced a dose-dependent increase in steady-state [Ca2+]i in Fura-2-loaded rat VSM cells.	Fura-2	76-82	endothelin 1	Rattus norvegicus	0-4
3202164-4	1988	Additionally NPY increased intracellular Ca2+, as assessed by fura-2 fluorescence, with a similar EC50.	Fura-2	62-68	neuropeptide Y	Homo sapiens	13-16
3177629-6	1988	Dopamine release by PC-12 cells challenged with (R)PAF is associated with a rapid 45Ca influx and efflux and a rise in cytoplasmic calcium concentrations ([Ca2+]i) evaluated by using the calcium indicators fura-2 and quin2.	Fura-2	206-212	PCNA clamp associated factor	Rattus norvegicus	51-54
3263369-4	1988	Intact fura-2 loaded cells stimulated by thrombin, a physiological agent that causes phosphatidylinositol 4,5-bisphosphate hydrolysis and Ins (1,4,5)P3 release, responded with a rise in cytoplasmic free Ca2+ levels that was dose dependently inhibited by W7(Ki = 25 microM), W13 (Ki = 45 microM), and CGS 9343B (Ki = 110 microM).	Fura-2	7-13	coagulation factor II	Rattus norvegicus	41-49
3063257-10	1988	In Fura-2-loaded cells GDP[S] inhibited thrombin-induced Ca2+ mobilization, as measured by Fura-2 fluorescence, in a dose-dependent manner.	Fura-2	3-9	coagulation factor II, thrombin	Homo sapiens	40-48
3063257-10	1988	In Fura-2-loaded cells GDP[S] inhibited thrombin-induced Ca2+ mobilization, as measured by Fura-2 fluorescence, in a dose-dependent manner.	Fura-2	91-97	coagulation factor II, thrombin	Homo sapiens	40-48
3170551-6	1988	Carbachol (10 microM) or ionomycin (10 microM) stimulation of fura-2-containing cells resulted in a rapid increase in cytosolic free Ca2+ concentration and in the extent of myosin light chain phosphorylation.	Fura-2	62-68	myosin heavy chain 14	Homo sapiens	173-179
2846829-8	1988	In fura-2 loaded RBL-1 cells, LTD4 and LTE4 induced a transient intracellular Ca++ mobilization.	Fura-2	3-9	RB transcriptional corepressor like 1	Rattus norvegicus	17-22
3263034-5	1988	In addition, bradykinin and melittin stimulated an increase in intracellular calcium concentration in fura-2 loaded endothelial cells and an increase in inositol 1,4,5-trisphosphate (Ins[1,4,5]P3) in cells prelabeled with 3H-myoinositol.	Fura-2	102-108	kininogen 1	Bos taurus	13-23
3263034-6	1988	Bradykinin stimulation produced transient increases in Ins(1,4,5)P3, fura-2 fluorescence and transient EDRF release.	Fura-2	69-75	kininogen 1	Bos taurus	0-10
3138102-4	1988	[Ca2+]i, measured with the fluorescent dye fura-2, was stimulated by oxytocin and vasopressin (oxytocin greater than vasopressin) in a dose-dependent manner.	Fura-2	43-49	arginine vasopressin	Homo sapiens	82-93
3196311-4	1988	Here, we have used Mn2+ as the extracellular bivalent cation which can bind to the fluorescent Ca2+ indicator fura-2 to quench its fluorescence completely.	Fura-2	110-116	carbonic anhydrase 2	Homo sapiens	95-98
3196311-5	1988	Human umbilical-vein endothelial cells were loaded with fura-2 and, in the presence of extracellular Mn2+, thrombin and histamine were shown to cause quenching of the intracellular dye.	Fura-2	56-62	coagulation factor II, thrombin	Homo sapiens	107-115
3143354-1	1988	The kinetic features of the changes in the cytosolic free Ca2+ concentration, [Ca2+]i, following stimulation by thyrotropin releasing hormone (TRH) were analysed in single cells of a pituitary line (GH3B6) by dual excitation microfluorimetry [Tsien, Rink & Poenie (1985) Cell Calcium 6, 145-157], using fura 2 as intracellular Ca2+ probe.	Fura-2	307-313	thyrotropin releasing hormone	Homo sapiens	143-146
3138102-4	1988	[Ca2+]i, measured with the fluorescent dye fura-2, was stimulated by oxytocin and vasopressin (oxytocin greater than vasopressin) in a dose-dependent manner.	Fura-2	43-49	arginine vasopressin	Homo sapiens	117-128
2851595-2	1988	Fura-2-loaded T51B cells responded with an increase in [Ca2+]i to EGF (5-50 ng/ml) that was blocked by low (25 microM) extracellular Ca2+ or by pretreatment with 50 microM La3+ to inhibit plasma membrane Ca2+ flux.	Fura-2	0-6	epidermal growth factor	Homo sapiens	66-69
3138987-1	1988	Thapsigargin, a sesquiterpene lactone with potent irritant and tumour-promoting activities, stimulates a rapid (within 15 s) transient increase in intracellular [Ca2+] in the NG115-401L neural cell line, as measured by the fluorescent indicator dye fura-2.	Fura-2	249-255	carbonic anhydrase 2	Mus musculus	162-165
3408504-1	1988	The effect of platelet-activating factor (PAF-acether) on cytosolic free calcium, [Ca2+]i, in adherent human vascular endothelial cells in culture was directly determined using a new fluorescent calcium indicator, fura-2.	Fura-2	214-220	PCNA clamp associated factor	Homo sapiens	42-45
3136667-2	1988	In the present study, measurement of BK-induced changes in fura-2 fluorescence and 86Rb+ efflux were used to monitor changes in cytosolic Ca2+ and K+ permeability in cultured bovine aortic endothelial cells.	Fura-2	59-65	kininogen 1	Bos taurus	37-39
3064899-0	1988	[Ca2+]i in single isolated cardiac cells: a review of recent results obtained with digital imaging microscopy and fura-2.	Fura-2	114-120	carbonic anhydrase 2	Homo sapiens	1-4
3064899-2	1988	In the studies reviewed in this article, [Ca2+]i in single isolated mammalian ventricular myocytes was observed through the use of the fluorescent Ca2+ indicator, fura-2.	Fura-2	163-169	carbonic anhydrase 2	Homo sapiens	42-45
3064899-2	1988	In the studies reviewed in this article, [Ca2+]i in single isolated mammalian ventricular myocytes was observed through the use of the fluorescent Ca2+ indicator, fura-2.	Fura-2	163-169	carbonic anhydrase 2	Homo sapiens	147-150
3261750-5	1988	Upon binding to gamma/delta-TCR-bearing Molt-13 cells and PBL, delta TCS1 elicited a fura-2 Caa+ signal indicating that the gamma/delta-receptor is functionally similar to the alpha/beta-heterodimer.	Fura-2	85-91	treacle ribosome biogenesis factor 1	Homo sapiens	69-73
3399509-1	1988	The cytosolic calcium (Ca2+i) response to angiotensin II (Ang II) was examined in single rat zona glomerulosa cells by monitoring fura-2 fluorescence with microspectrofluorimetry.	Fura-2	130-136	angiotensinogen	Rattus norvegicus	42-56
3399509-1	1988	The cytosolic calcium (Ca2+i) response to angiotensin II (Ang II) was examined in single rat zona glomerulosa cells by monitoring fura-2 fluorescence with microspectrofluorimetry.	Fura-2	130-136	angiotensinogen	Rattus norvegicus	58-64
2450043-0	1988	Parallel measurement of oxoglutarate dehydrogenase activity and matrix free Ca2+ in fura-2-loaded heart mitochondria.	Fura-2	84-90	oxoglutarate dehydrogenase	Homo sapiens	24-50
3260903-2	1988	Thrombin (0.003-3.0 U/ml) and histamine (10(-7)-10(-4) M) induced rapid increases in [Ca2+]i in suspended cells as monitored with the fluorescent calcium indicator fura-2.	Fura-2	164-170	coagulation factor II, thrombin	Homo sapiens	0-8
3130276-2	1988	Furthermore, this study, using 45Ca2+, aequorin and fura-2, revealed the mechanism involved in intracellular Ca2+ shifts caused by gastrin and the involvements of inositol 1,4,5-trisphosphate (IP3) and diacylglycerol in producing those shifts.	Fura-2	52-58	gastrin	Cavia porcellus	131-138
3360779-2	1988	In platelets, thrombin also stimulates Mn2+ entry into the cytosol; this is seen as a stimulated quench of fura-2 fluorescence.	Fura-2	107-113	coagulation factor II, thrombin	Homo sapiens	14-22
2451660-5	1988	Measurement of intracellular free Ca2+ with fura-2 demonstrated that TGF beta treatment markedly increased the EGF-stimulated rise in free Ca2+ and increased the duration of the response.	Fura-2	44-50	transforming growth factor, beta 1	Rattus norvegicus	69-77
3162323-1	1988	The voltage dependence of the intracellular Ca2+ transients was measured in single rat ventricular myocytes with the fluorescent Ca2+ indicator dye fura-2.	Fura-2	148-154	carbonic anhydrase 2	Rattus norvegicus	44-47
3162323-1	1988	The voltage dependence of the intracellular Ca2+ transients was measured in single rat ventricular myocytes with the fluorescent Ca2+ indicator dye fura-2.	Fura-2	148-154	carbonic anhydrase 2	Rattus norvegicus	129-132
2455515-2	1988	Addition of IFN to the cells loaded with Fura-2, a fluorescent Ca2+ indicator, causes an immediate increase in [Ca2+]i, which is the earliest event after IFN stimulation.	Fura-2	41-47	interferon alpha 1	Homo sapiens	12-15
2455515-2	1988	Addition of IFN to the cells loaded with Fura-2, a fluorescent Ca2+ indicator, causes an immediate increase in [Ca2+]i, which is the earliest event after IFN stimulation.	Fura-2	41-47	interferon alpha 1	Homo sapiens	154-157
2967092-9	1988	Cholecystokinin induced a rapid increase in cytoplasmic Ca2+ ([Ca2+]i) as estimated with fura-2 and aequorin.	Fura-2	89-95	cholecystokinin	Homo sapiens	0-15
2967092-9	1988	Cholecystokinin induced a rapid increase in cytoplasmic Ca2+ ([Ca2+]i) as estimated with fura-2 and aequorin.	Fura-2	89-95	carbonic anhydrase 2	Homo sapiens	56-59
2967092-9	1988	Cholecystokinin induced a rapid increase in cytoplasmic Ca2+ ([Ca2+]i) as estimated with fura-2 and aequorin.	Fura-2	89-95	carbonic anhydrase 2	Homo sapiens	63-66
2967092-14	1988	It appears that the late component of the Ca2+ transient is dependent on Ca2+ influx from the extracellular space and is too localized to be detected by fura-2.	Fura-2	153-159	carbonic anhydrase 2	Homo sapiens	42-45
3258153-4	1988	Ten nM EGF increases [Ca2+]i, measured using fura-2, a fluorescent Ca2+ indicator; the EGF-induced [Ca2+]i response occurs immediately, reaches a maximum within several seconds, and then slowly declines.	Fura-2	45-51	epidermal growth factor	Homo sapiens	7-10
3258153-4	1988	Ten nM EGF increases [Ca2+]i, measured using fura-2, a fluorescent Ca2+ indicator; the EGF-induced [Ca2+]i response occurs immediately, reaches a maximum within several seconds, and then slowly declines.	Fura-2	45-51	epidermal growth factor	Homo sapiens	87-90
2450043-3	1988	In this paper the correlation between the [Ca2+]m and the in situ activity of oxoglutarate dehydrogenase (OGDH) in fura-2-loaded mitochondria is shown.	Fura-2	115-121	oxoglutarate dehydrogenase	Homo sapiens	78-104
2450043-3	1988	In this paper the correlation between the [Ca2+]m and the in situ activity of oxoglutarate dehydrogenase (OGDH) in fura-2-loaded mitochondria is shown.	Fura-2	115-121	oxoglutarate dehydrogenase	Homo sapiens	106-110
2829732-1	1988	In platelets that were loaded with the intracellular calcium indicator fura2, ethanol induced a rapid mobilization of calcium from internal, thrombin-sensitive pools.	Fura-2	71-76	coagulation factor II, thrombin	Homo sapiens	141-149
2828915-2	1988	In cells loaded with Fura 2, we found that 1 microM oxytocin induced a rapid increase in cytosolic free [Ca2+]i from 120 nM to 250 nM within 12 sec.	Fura-2	21-27	oxytocin/neurophysin I prepropeptide	Sus scrofa	52-60
2826882-27	1988	In FMLP stimulated neutrophils, the presence of ATP or ADP leads to enhanced increases in intracellular levels of Ca++ as determined by the fura-2 probe, while the presence of AMP or adenosine results in inhibition of the increases in FMLP induced elevations in cytosolic Ca++.	Fura-2	140-146	formyl peptide receptor 1	Homo sapiens	3-7
2469884-2	1988	The experiments were carried out on cells loaded with fura-2 using a digital photon-counting camera (PMI VIM) with computer analysis of 16-bit images.	Fura-2	54-60	vimentin	Rattus norvegicus	105-108
3385691-2	1988	The calcium dependence on the excitation spectrum of fura 2 allows us to choose two wavelengths, lambda 1 and lambda 2, at which an increase in [Ca2+]i causes either a rise or a fall in fluorescence; the ratio of fluorescence at lambda 1 and lambda 2 (R = F lambda 1/F lambda 2) is a measure of [Ca2+]i.	Fura-2	53-59	immunoglobulin lambda like polypeptide 2, pseudogene	Homo sapiens	256-266
3342003-1	1988	Intracellular free Ca2+ was monitored in suspensions of 1321N1 astrocytoma cells by using the Ca2+ indicator fura-2.	Fura-2	109-115	carbonic anhydrase 2	Homo sapiens	19-22
3342003-1	1988	Intracellular free Ca2+ was monitored in suspensions of 1321N1 astrocytoma cells by using the Ca2+ indicator fura-2.	Fura-2	109-115	carbonic anhydrase 2	Homo sapiens	94-97
3236338-5	1988	Use of fura2, along with the capacity of Mn2+ ions to effectively quench fura2 fluorescence, provided the basis for a simple and decisive method to determine whether the added CCl4 was permeabilizing the hepatocyte plasma membrane by direct solvent action.	Fura-2	7-12	C-C motif chemokine ligand 4	Rattus norvegicus	176-180
3236338-5	1988	Use of fura2, along with the capacity of Mn2+ ions to effectively quench fura2 fluorescence, provided the basis for a simple and decisive method to determine whether the added CCl4 was permeabilizing the hepatocyte plasma membrane by direct solvent action.	Fura-2	73-78	C-C motif chemokine ligand 4	Rattus norvegicus	176-180
3347929-6	1988	When effects of cerulenin on intracellular Ca2+ concentration were examined, mobilization of intracellular Ca2+ by thrombin was significantly depressed in the cerulenin-treated platelets as judged by Fura2, Quin2 or chlortetracycline fluorescence.	Fura-2	200-205	coagulation factor II, thrombin	Homo sapiens	115-123
2451743-8	1987	Thrombin promoted the efflux of 45Ca2+ from cells co-loaded with fura-2 and the isotope.	Fura-2	65-71	coagulation factor II, thrombin	Homo sapiens	0-8
2962504-2	1987	Resting Ca levels, measured by the use of fura-2, were dose-dependently decreased by up to 30%, by atriopeptin 23.	Fura-2	42-48	natriuretic peptide A	Homo sapiens	99-110
2443131-1	1987	The ability of bombesin-like peptides to elevate intracellular Ca2+ levels in small cell lung cancer cells was investigated using the fluorescent Ca2+ indicator Fura 2.	Fura-2	161-167	gastrin releasing peptide	Homo sapiens	15-23
2830242-2	1987	Further findings as to fura2-loaded platelets offered additional support, demonstrating that the initial activation of phospholipase A2 (PLA2) does not require cytoplasmic Ca2+ mobilization.	Fura-2	23-28	phospholipase A2 group IB	Rattus norvegicus	119-135
2830242-2	1987	Further findings as to fura2-loaded platelets offered additional support, demonstrating that the initial activation of phospholipase A2 (PLA2) does not require cytoplasmic Ca2+ mobilization.	Fura-2	23-28	phospholipase A2 group IB	Rattus norvegicus	137-141
3323675-7	1987	The intracellular calcium chelator fura 2 also significantly inhibited LIF-mediated degranulation (61.8% inhibition).	Fura-2	35-41	LIF interleukin 6 family cytokine	Homo sapiens	71-74
2444358-2	1987	After bradykinin (100 nM) exposure, endothelial cell associated fura-2 fluorescence peaked within 10-20 seconds and then declined to a steady level 2- to 3-fold above resting values.	Fura-2	64-70	kininogen 1	Bos taurus	6-16
2444358-4	1987	Chelation of extracellular calcium with EGTA before addition of bradykinin resulted in a substantial decrease in the fura-2 transient and elimination of the long-lasting component.	Fura-2	117-123	kininogen 1	Bos taurus	64-74
2824759-7	1987	Anagrelide also inhibited the rise in intracellular Ca++ caused by thrombin, as measured using Fura-2-loaded platelets.	Fura-2	95-101	coagulation factor II, thrombin	Homo sapiens	67-75
2446886-3	1987	Platelet stimulation with thrombin, adenosine diphosphate, platelet activating factor and arachidonic acid resulted in a concentration-dependent 2.5-3-fold increase in cytoplasmic free calcium concentration over the basal levels (140 +/- 32 nM or 104 +/- 21 respectively) as measured with the fluorescent dyes Quin-2 and Fura-2.	Fura-2	321-327	coagulation factor II, thrombin	Homo sapiens	26-34
3498624-2	1987	Relationships between extracellular calcium ([Ca2+]e), cytosolic free calcium ([Ca2+]i) (as measured with fura-2), and secretion of immunoreactive CGRP and CT have been investigated in rat and human MTC cell lines.	Fura-2	106-112	calcitonin-related polypeptide alpha	Rattus norvegicus	147-151
3323675-2	1987	Using the fluorescent probe fura 2, we demonstrated that LIF (1/2-2 units) induced a small but significant dose-dependent increase in intracellular calcium in the presence of both calcium-containing (71 nM to 158 nM) and calcium-free (53 nM to 144 nM) buffer solutions.	Fura-2	28-34	LIF interleukin 6 family cytokine	Homo sapiens	57-60
3653395-1	1987	In the isolated parietal cell the following observations were made: gastrin led to an increase in cytoplasmic free Ca2+ concentration ([Ca2+]i) even in the absence of medium Ca2+ and to transient membrane depolarization in both the absence of the medium Ca2+ and the fura-2-loaded cell.	Fura-2	267-273	gastrin	Homo sapiens	68-75
3040727-6	1987	Pretreatment with PMA consistently shortened the onset time of the neutrophil"s responses to agonists by about 1 s. When H2O2 production was initiated with PMA, a subsequent stimulation with the agonist fMLP elicited an immediate response (onset time less than 0.2 s) which preceded further changes in fura-2-detected [Ca2+]i.	Fura-2	302-308	formyl peptide receptor 1	Homo sapiens	203-207
3032310-6	1987	Thrombin-stimulated rise in platelet cytosolic Ca2+, measured by fura2 fluorescence, and thromboxane A2 formation, measured by radioimmunoassay (RIA), are likewise synergistically inhibited by PGI2 and plasmin.	Fura-2	65-70	coagulation factor II, thrombin	Homo sapiens	0-8
3672425-2	1987	This was shown by increased aggregatory and secretory responses of quin2 or fura-2 loaded platelets after stimulation with ADP, PAF and with low concentrations of thrombin, collagen, the endoperoxide analog U-46619 and the calcium ionophore A 23187.	Fura-2	76-82	coagulation factor II, thrombin	Homo sapiens	163-171
2439506-3	1987	By using the Ca2+ indicators quin2 and fura-2, we demonstrate that in PC12 cells voltage-gated Ca2+ channels undergo inactivation dependent on both voltage and [Ca2+]i. Inactivation, however, is never complete and a small number of channels remains open during prolonged depolarization, explaining the steady state elevation of [Ca2+]i observed in cells depolarized with high KCl.	Fura-2	39-45	carbonic anhydrase 2	Rattus norvegicus	95-98
2439506-3	1987	By using the Ca2+ indicators quin2 and fura-2, we demonstrate that in PC12 cells voltage-gated Ca2+ channels undergo inactivation dependent on both voltage and [Ca2+]i. Inactivation, however, is never complete and a small number of channels remains open during prolonged depolarization, explaining the steady state elevation of [Ca2+]i observed in cells depolarized with high KCl.	Fura-2	39-45	carbonic anhydrase 2	Rattus norvegicus	95-98
2439506-3	1987	By using the Ca2+ indicators quin2 and fura-2, we demonstrate that in PC12 cells voltage-gated Ca2+ channels undergo inactivation dependent on both voltage and [Ca2+]i. Inactivation, however, is never complete and a small number of channels remains open during prolonged depolarization, explaining the steady state elevation of [Ca2+]i observed in cells depolarized with high KCl.	Fura-2	39-45	carbonic anhydrase 2	Rattus norvegicus	95-98
2440616-1	1987	[Ca2+]i transients, elicited by voltage-clamp depolarization of single guinea pig cardiac ventricular cells, were observed through use of the fluorescent Ca2+ indicator, fura-2.	Fura-2	170-176	LOW QUALITY PROTEIN: carbonic anhydrase 2	Cavia porcellus	1-4
2440616-1	1987	[Ca2+]i transients, elicited by voltage-clamp depolarization of single guinea pig cardiac ventricular cells, were observed through use of the fluorescent Ca2+ indicator, fura-2.	Fura-2	170-176	LOW QUALITY PROTEIN: carbonic anhydrase 2	Cavia porcellus	154-157
2885709-6	1987	Thus, fura-2 appears to be an adequate probe for resolving second-to-second changes in [Ca++]i induced by hormone receptor activation in anterior pituitary cells.	Fura-2	6-12	carbonic anhydrase 1	Rattus norvegicus	88-94
3758190-1	1986	We have investigated the effects of substituting extracellular Na+ by choline or K+ on responses of quin2- and fura-2-loaded human platelets to thrombin and platelet-activating factor (PAF).	Fura-2	111-117	coagulation factor II, thrombin	Homo sapiens	144-152
3494624-8	1987	These results, as well as additional microscopic fura-2 results in Swiss 3T3 fibroblasts, demonstrate that the Ca2+ signal elicited by EGF is due to two components: redistribution from an intracellular store (possibly mediated by generation of inositol trisphosphate) and stimulated influx across the plasmalemma.	Fura-2	49-55	epidermal growth factor	Mus musculus	135-138
2884101-2	1987	Changes in intracellular Ca2+, measured using the fluorescent indicator dye Fura-2, show two components; an initial rapid peak in [Ca2+]i which is essentially independent of extracellular Ca2+, and a sustained plateau dependent on the presence of extracellular Ca2+.	Fura-2	76-82	carbonic anhydrase 2	Mus musculus	25-28
2884101-2	1987	Changes in intracellular Ca2+, measured using the fluorescent indicator dye Fura-2, show two components; an initial rapid peak in [Ca2+]i which is essentially independent of extracellular Ca2+, and a sustained plateau dependent on the presence of extracellular Ca2+.	Fura-2	76-82	carbonic anhydrase 2	Mus musculus	131-134
2884101-2	1987	Changes in intracellular Ca2+, measured using the fluorescent indicator dye Fura-2, show two components; an initial rapid peak in [Ca2+]i which is essentially independent of extracellular Ca2+, and a sustained plateau dependent on the presence of extracellular Ca2+.	Fura-2	76-82	carbonic anhydrase 2	Mus musculus	131-134
2884101-2	1987	Changes in intracellular Ca2+, measured using the fluorescent indicator dye Fura-2, show two components; an initial rapid peak in [Ca2+]i which is essentially independent of extracellular Ca2+, and a sustained plateau dependent on the presence of extracellular Ca2+.	Fura-2	76-82	carbonic anhydrase 2	Mus musculus	131-134
3112407-2	1987	Using isolated epithelial cells from the toad urinary bladder, Caf has been measured using the intracellular Ca-sensitive fluorescent dyes Fura 2 and Quin 2.	Fura-2	139-145	lysine acetyltransferase 2B	Homo sapiens	63-66
3755550-1	1986	Continuous measurement and imaging of the intracellular free calcium ion concentration ([Ca2+]i) of mitotic and interphase PtK1 cells was accomplished with the new fluorescent Ca2+ indicator fura-2.	Fura-2	191-197	mitogen-activated protein kinase kinase kinase 11	Homo sapiens	123-127
3102480-4	1987	EGF and bradykinin stimulated the rapid accumulation of the two isomers of InsP3, Ins(1,3,4)P3, and Ins(1,4,5)P3 as determined by high performance liquid chromatography analysis; maximal accumulation of Ins(1,4,5)P3 occurred within 15 s. EGF and bradykinin also stimulated a rapid (maximal levels attained within 30 s after addition of hormone) and a sustained 4- and 6-fold rise, respectively, in cytosolic free Ca2+ levels as measured by Fura-2 fluorescence.	Fura-2	440-446	epidermal growth factor	Homo sapiens	0-3
3102480-4	1987	EGF and bradykinin stimulated the rapid accumulation of the two isomers of InsP3, Ins(1,3,4)P3, and Ins(1,4,5)P3 as determined by high performance liquid chromatography analysis; maximal accumulation of Ins(1,4,5)P3 occurred within 15 s. EGF and bradykinin also stimulated a rapid (maximal levels attained within 30 s after addition of hormone) and a sustained 4- and 6-fold rise, respectively, in cytosolic free Ca2+ levels as measured by Fura-2 fluorescence.	Fura-2	440-446	kininogen 1	Homo sapiens	8-18
3792558-0	1987	Stimulation of Ca2+ efflux from fura-2-loaded platelets activated by thrombin or phorbol myristate acetate.	Fura-2	32-38	coagulation factor II, thrombin	Homo sapiens	69-77
3094510-0	1986	Thrombin and ionomycin can raise platelet cytosolic Ca2+ to micromolar levels by discharge of internal Ca2+ stores: studies using fura-2.	Fura-2	130-136	coagulation factor II, thrombin	Homo sapiens	0-8
3094510-4	1986	These results with fura-2-loaded platelets indicate that mobilisation of internal Ca2+ can contribute a substantial proportion of the early peak [Ca2+]i evoked by thrombin directly confirming the deductions from previous work with different loadings of quin2.	Fura-2	19-25	coagulation factor II, thrombin	Homo sapiens	163-171
3758190-1	1986	We have investigated the effects of substituting extracellular Na+ by choline or K+ on responses of quin2- and fura-2-loaded human platelets to thrombin and platelet-activating factor (PAF).	Fura-2	111-117	PCNA clamp associated factor	Homo sapiens	157-183
3758190-1	1986	We have investigated the effects of substituting extracellular Na+ by choline or K+ on responses of quin2- and fura-2-loaded human platelets to thrombin and platelet-activating factor (PAF).	Fura-2	111-117	PCNA clamp associated factor	Homo sapiens	185-188
3082623-1	1986	The calcium concentration, [Ca]i, in single rat peritoneal mast cells was measured by means of the new Ca indicator dye fura-2.	Fura-2	120-126	carbonic anhydrase 1	Rattus norvegicus	28-32
3014901-6	1986	With fura-2 as the intracellular Ca2+ indicator, [Ca2+]f increased from 74 +/- 7 to a peak of 578 +/- 39 nM (n = 17) with 100 nM AVP.	Fura-2	5-11	arginine vasopressin	Rattus norvegicus	129-132
3755042-0	1986	Kinetic differences between thrombin-induced and ADP-induced calcium influx and release from internal stores in fura-2-loaded human platelets.	Fura-2	112-118	coagulation factor II, thrombin	Homo sapiens	28-36
3460085-1	1986	We have used a Ca2+-sensitive dye, fura-2, to investigate the role of Ca2+ during mitosis in Pt K2 epithelial cells.	Fura-2	35-41	carbonic anhydrase 2	Homo sapiens	15-18
3004360-2	1986	Cytosolic [Ca2+] was measured using either quin 2 or the recently described indicator fura 2.	Fura-2	86-92	carbonic anhydrase 2	Mus musculus	11-14
3840688-4	1985	Platelets loaded with Fura 2 showed a significant rise in cytosolic calcium when stirred with agonists such as epinephrine, arachidonate and thrombin, whereas Quin 2 loaded platelets demonstrated a rise in cytosolic calcium only with thrombin stimulation.	Fura-2	22-28	coagulation factor II, thrombin	Homo sapiens	141-149