PMID-sentid	Pub_year	Sent_text	compound_name	comp_offset	prot_official_name	organism	prot_offset
9466702-2	1997	The nature of this late developmental change in Ca2+ sensitivity for GFAP phosphorylation was investigated in the present work by comparing in immature and adult animals phosphorylation of GFAP by endogenous protein kinase activity in cytoskeletal fractions and tryptic phosphopeptide maps prepared from cytoskeletal fractions labelled with [gamma-32P]ATP and from slices labelled with [32P]phosphate.	gamma-32p	342-351	glial fibrillary acidic protein	Rattus norvegicus	69-73
9915773-9	1999	This was further corroborated by an increased incorporation of the photoreactive GTP analog [gamma-32P]GTP azidoanilide into alpha subunits of Gi2 and Gi3 after beta2AR stimulation by zinterol or isoproterenol plus the beta1AR blocker CGP 20712A.	gamma-32p	93-102	adrenergic receptor, beta 2	Mus musculus	161-168
9915773-9	1999	This was further corroborated by an increased incorporation of the photoreactive GTP analog [gamma-32P]GTP azidoanilide into alpha subunits of Gi2 and Gi3 after beta2AR stimulation by zinterol or isoproterenol plus the beta1AR blocker CGP 20712A.	gamma-32p	93-102	adrenergic receptor, beta 1	Mus musculus	219-226
9771928-2	1998	Gel mobility shift assays using a gamma-32P-labelled NF-kappaB oligonucleotide probe showed that treatment of HepG2 cells with quercetin (up to 10 microM, sub-cytotoxic) did not elevate NF-kappaB binding activity of nuclear extract protein but did inhibit binding activity of an extract from cells treated with the oxidant H2O2.	gamma-32p	34-43	nuclear factor kappa B subunit 1	Homo sapiens	53-62
9466702-2	1997	The nature of this late developmental change in Ca2+ sensitivity for GFAP phosphorylation was investigated in the present work by comparing in immature and adult animals phosphorylation of GFAP by endogenous protein kinase activity in cytoskeletal fractions and tryptic phosphopeptide maps prepared from cytoskeletal fractions labelled with [gamma-32P]ATP and from slices labelled with [32P]phosphate.	gamma-32p	342-351	glial fibrillary acidic protein	Rattus norvegicus	189-193
9277354-4	1997	Nuclear proteins from flow-conditioned human aortic endothelial cells (HAEC) were analyzed by electrophoretic mobility shift assay using [gamma-32P]dATP-labeled NF-kappa B-specific oligonucleotide.	gamma-32p	138-147	nuclear factor kappa B subunit 1	Homo sapiens	161-171
9063576-7	1997	Ca2+/calmodulin dependent [gamma-32P] incorporation was dramatically decreased in yeast cells submitted to a heat treatment.	gamma-32p	27-36	calmodulin	Saccharomyces cerevisiae S288C	5-15
8561803-4	1996	By labeling the c-Ret protein immunoprecipitated from tumor tissues with [gamma-32P]ATP in vitro, its homodimers were detected in pheochromocytomas from MEN 2A patients but not in a sporadic tumor.	gamma-32p	74-83	ret proto-oncogene	Homo sapiens	18-21
7636203-8	1995	In addition, anti-Lck Ab immunoprecipitated a phosphorylated 60-kDa protein from gamma-32P-labeled T-TIL that was not seen in normal resting T cells.	gamma-32p	81-90	LCK proto-oncogene, Src family tyrosine kinase	Homo sapiens	18-21
7493994-10	1995	Phosphorylation of pleckstrin could be induced using permeabilized platelets supplied with exogenous gamma-32P[ATP] and synthetic dipalmitoyl PtdIns-3,4,5-P3 and dipalmitoyl PtdIns-3,4-P2 micelles, but not with dipalmitoyl phosphatidylinositol 3-phosphate or phosphatidylinositol 4,5-bisphosphate.	gamma-32p	101-110	pleckstrin	Homo sapiens	19-29
8048945-1	1994	After incubation of high mobility group (HMG) proteins 1 and 2 with DNA-dependent protein kinase (DNA-PK) and [gamma-32P]ATP in the presence of double-stranded DNA, not only phosphorylation of HMG proteins but also enhancement of autophosphorylation of the catalytic polypeptide of 350 kDa in DNA-PK was observed.	gamma-32p	111-120	high mobility group box 2	Homo sapiens	20-62
7530719-7	1995	To identify potential additional sites responsible for the residual activity, large amounts of this mutant CFTR were phosphorylated with PKA using high specific activity [gamma-32P]ATP.	gamma-32p	171-180	CF transmembrane conductance regulator	Homo sapiens	107-111
8048945-1	1994	After incubation of high mobility group (HMG) proteins 1 and 2 with DNA-dependent protein kinase (DNA-PK) and [gamma-32P]ATP in the presence of double-stranded DNA, not only phosphorylation of HMG proteins but also enhancement of autophosphorylation of the catalytic polypeptide of 350 kDa in DNA-PK was observed.	gamma-32p	111-120	protein kinase, DNA-activated, catalytic subunit	Homo sapiens	293-299
8510223-9	1993	Immunoprecipitation of transfected COS-1 cell lysates with antihemagglutinin or anti-Tpl-2 antibodies, followed by incubation with [gamma-32P]ATP, confirmed that Tpl-2 possesses protein kinase activity.	gamma-32p	132-141	mitogen-activated protein kinase kinase kinase 8	Homo sapiens	162-167
8027592-5	1994	The Hu-IFN-alpha B2-P and Hu-IFN-alpha A/D-P can be phosphorylated by the catalytic subunit of the cAMP-dependent protein kinase and [gamma-32P]ATP with retention of biological activities.	gamma-32p	134-143	interferon alpha 2	Homo sapiens	7-16
8027592-5	1994	The Hu-IFN-alpha B2-P and Hu-IFN-alpha A/D-P can be phosphorylated by the catalytic subunit of the cAMP-dependent protein kinase and [gamma-32P]ATP with retention of biological activities.	gamma-32p	134-143	interferon alpha 2	Homo sapiens	29-38
8361641-3	1993	Whereas, the PKC activities, which were measured by the incorporation of [gamma-32P] into a specific PKC substrate peptide, in both the cytosolic and the particulate fractions did not change.	gamma-32p	74-83	protein kinase C, alpha	Rattus norvegicus	101-104
8439339-1	1993	The human epidermal growth factor receptor was detected immunohistochemically in the postacrosomal region of ejaculated human sperm and, when immunopurified and exposed to [gamma-32P]ATP, was found to be autophosphorylated.	gamma-32p	173-182	epidermal growth factor receptor	Homo sapiens	10-42
8429033-4	1993	GH-GH receptor complexes were immunoprecipitated with anti-GH antibody from GH-treated CHO4 cells and incubated with [gamma-32P]ATP.	gamma-32p	118-127	growth hormone receptor	Mus musculus	3-14
3105577-1	1987	In human placental membranes isolated in the presence of ethylenediaminetetraacetic acid (EDTA), epidermal growth factor (EGF) stimulated the [gamma-32P]ATP-dependent phosphorylation of tyrosine residues on the 170-kilodalton (kDa) EGF receptor and on a 35-kDa protein.	gamma-32p	143-152	epidermal growth factor	Homo sapiens	97-120
1652871-6	1991	The gene product of KIN1 was shown to be a serine/threonine-specific protein kinase in immune complexes, as determined by the transfer of label from [gamma-32P]ATP to either pp145KIN1 or to an exogenously added substrate, alpha-casein.	gamma-32p	150-159	serine/threonine protein kinase KIN1	Saccharomyces cerevisiae S288C	20-24
1429559-5	1992	Importantly, GP85 (CD44) appears to display a GTPase activity which hydrolyzes [gamma-32P]GTP at a rate of 0.011 mol of Pi released/mol of GP85 (CD44)/min.	gamma-32p	80-89	CD44 antigen	Mus musculus	19-23
1429559-5	1992	Importantly, GP85 (CD44) appears to display a GTPase activity which hydrolyzes [gamma-32P]GTP at a rate of 0.011 mol of Pi released/mol of GP85 (CD44)/min.	gamma-32p	80-89	CD44 antigen	Mus musculus	145-149
1612782-7	1992	The data suggest that ER of HI tumors is less phosphorylated in vivo than the ER of HD/HR tumors, so that the receptor of HI tumors is more susceptible to gamma-32P-ATP phosphorylation and ATP-induced hormone binding in vitro.	gamma-32p	155-164	estrogen receptor 1 (alpha)	Mus musculus	22-24
1612782-7	1992	The data suggest that ER of HI tumors is less phosphorylated in vivo than the ER of HD/HR tumors, so that the receptor of HI tumors is more susceptible to gamma-32P-ATP phosphorylation and ATP-induced hormone binding in vitro.	gamma-32p	155-164	estrogen receptor 1 (alpha)	Mus musculus	78-80
1533500-7	1992	Ca(2+)-ATPase inactivation is accompanied by incorporation of radioactivity from gamma-32P into an acid-precipitable enzyme.	gamma-32p	81-90	dynein axonemal heavy chain 8	Homo sapiens	7-13
1295538-3	1992	The bovine prolactin (PRL) mRNA was confirmed by Northern blot analysis of the mRNA with gamma-32P labelled synthetic oligonucleotide probes based on the partial amino acid sequences of bovine PRL and could stimulate the incorporation of 35S-methionine into protein in the rabbit reticulocyte cell-free system.	gamma-32p	89-98	prolactin	Bos taurus	11-20
1295538-3	1992	The bovine prolactin (PRL) mRNA was confirmed by Northern blot analysis of the mRNA with gamma-32P labelled synthetic oligonucleotide probes based on the partial amino acid sequences of bovine PRL and could stimulate the incorporation of 35S-methionine into protein in the rabbit reticulocyte cell-free system.	gamma-32p	89-98	prolactin	Bos taurus	22-25
1650365-4	1991	To address the functional significance of this modification, the stoichiometry of GPIIIa phosphorylation was determined in resting and activated platelets by estimating the specific activity of metabolic [gamma-32P]ATP from the specific activity of phosphatidic acid.	gamma-32p	205-214	integrin subunit beta 3	Homo sapiens	82-88
2121290-1	1990	The polypeptide with a mobility of the tryptophanyl-tRNA-synthetase subunit can be labeled in bovine pancreas extracts from [gamma-32P]ATP.	gamma-32p	125-134	tryptophanyl-tRNA synthetase 1	Bos taurus	39-67
3038872-1	1987	To identify the autophosphorylation sites on the human insulin receptor (IR), partially purified human IR was incubated in vitro in the presence of insulin and manganese [gamma-32P]ATP so as to achieve near-maximal activation of the histone 2b kinase activity.	gamma-32p	171-180	insulin receptor	Homo sapiens	55-71
3038872-1	1987	To identify the autophosphorylation sites on the human insulin receptor (IR), partially purified human IR was incubated in vitro in the presence of insulin and manganese [gamma-32P]ATP so as to achieve near-maximal activation of the histone 2b kinase activity.	gamma-32p	171-180	insulin receptor	Homo sapiens	73-75
3105577-1	1987	In human placental membranes isolated in the presence of ethylenediaminetetraacetic acid (EDTA), epidermal growth factor (EGF) stimulated the [gamma-32P]ATP-dependent phosphorylation of tyrosine residues on the 170-kilodalton (kDa) EGF receptor and on a 35-kDa protein.	gamma-32p	143-152	epidermal growth factor	Homo sapiens	122-125
3105577-1	1987	In human placental membranes isolated in the presence of ethylenediaminetetraacetic acid (EDTA), epidermal growth factor (EGF) stimulated the [gamma-32P]ATP-dependent phosphorylation of tyrosine residues on the 170-kilodalton (kDa) EGF receptor and on a 35-kDa protein.	gamma-32p	143-152	epidermal growth factor	Homo sapiens	232-235
3891757-3	1985	The subunit stoichiometry of these different high molecular weight forms of the insulin receptor were determined by comparisons of silver-stained gel profiles with the autoradiograms of 125I-insulin specifically cross-linked to the alpha subunit and [gamma-32P]ATP specifically autophosphorylated beta subunit gel profiles.	gamma-32p	251-260	insulin receptor	Homo sapiens	80-96
3036615-11	1987	Triton X-100 (0.15%) was necessary in the [1-3H]dolichol kinase assay (only 3% of enzymatic activity in the absence of detergent), while with [gamma-32P]CTP dolichol kinase detergent was only of minor influence (30% stimulation at 0.02% Triton X-100).	gamma-32p	143-152	solute carrier family 25 member 1	Bos taurus	153-156
3466646-4	1986	Competition binding experiments were carried out by adding an analogue, [alpha-32P]GTP, and a catalytic amount of photoexcited rhodopsin (R) to transducin and measuring the amount of bound [gamma-32P]GTP.	gamma-32p	190-199	rhodopsin	Homo sapiens	127-136
2871546-2	1985	Increasing the time of incubation of hippocampal synaptic plasma membranes with the peptide from 15 sec to 30 min prior to addition of 7.5 microM [gamma-32P]ATP results in a complete reduction of B50 phosphorylation.	gamma-32p	147-156	growth associated protein 43	Homo sapiens	196-199
3014890-4	1986	IGF I-stimulated phosphorylation of a 92,000 Mr protein was effected in detergent-solubilized membranes incubated with 100 microM [gamma-32P]ATP.	gamma-32p	131-140	insulin like growth factor 1	Canis lupus familiaris	0-5
278983-5	1978	Phosphorylation of HMG-CoA reductase was confirmed by immunoprecipitation of partially purified [gamma-32P]-ATP+Mg2+-inactivated microsomal HMG-CoA reductase with a reductase-specific antiserum.	gamma-32p	97-106	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	19-36
6093355-1	1984	The gag-mos hybrid protein encoded by ts110 MoMuSV was shown to have an associated protein kinase activity which phosphorylated both P85gag-mos and P58gag when [gamma-32P]ATP and a manganese cofactor were added to an immune complex containing P85gag-mos.	gamma-32p	161-170	MOS proto-oncogene, serine/threonine kinase	Homo sapiens	8-11
6093355-1	1984	The gag-mos hybrid protein encoded by ts110 MoMuSV was shown to have an associated protein kinase activity which phosphorylated both P85gag-mos and P58gag when [gamma-32P]ATP and a manganese cofactor were added to an immune complex containing P85gag-mos.	gamma-32p	161-170	MOS proto-oncogene, serine/threonine kinase	Homo sapiens	140-143
6093355-1	1984	The gag-mos hybrid protein encoded by ts110 MoMuSV was shown to have an associated protein kinase activity which phosphorylated both P85gag-mos and P58gag when [gamma-32P]ATP and a manganese cofactor were added to an immune complex containing P85gag-mos.	gamma-32p	161-170	MOS proto-oncogene, serine/threonine kinase	Homo sapiens	140-143
2931114-2	1985	Cross-linked acto-SF-1 plus [gamma-32P]ATP reaction mixture milliseconds old was quenched either in a large excess of unlabeled ATP (ATP chase) or in acid (Pi burst).	gamma-32p	29-38	splicing factor 1	Homo sapiens	18-22
6314330-1	1983	The molecular mechanism of growth hormone release by synthetic somatocrinin was investigated on purified hog anterior pituitary secretory granules; the granules were found to contain a cAMP-dependent protein kinase that catalyzed [gamma-32P]-ATP histone phosphorylation with maximal rates ranging from 1 to 5 nmol of Pi incorporated per mg of protein per 20 min.	gamma-32p	231-240	growth hormone 1	Homo sapiens	27-41
6314330-1	1983	The molecular mechanism of growth hormone release by synthetic somatocrinin was investigated on purified hog anterior pituitary secretory granules; the granules were found to contain a cAMP-dependent protein kinase that catalyzed [gamma-32P]-ATP histone phosphorylation with maximal rates ranging from 1 to 5 nmol of Pi incorporated per mg of protein per 20 min.	gamma-32p	231-240	growth hormone releasing hormone	Homo sapiens	63-75
278983-5	1978	Phosphorylation of HMG-CoA reductase was confirmed by immunoprecipitation of partially purified [gamma-32P]-ATP+Mg2+-inactivated microsomal HMG-CoA reductase with a reductase-specific antiserum.	gamma-32p	97-106	3-hydroxy-3-methylglutaryl-CoA reductase	Homo sapiens	140-157
16549426-4	2006	Incubation of Akt immunoprecipitated from transfected cells with a pre-activated Akt recombinant protein and gamma-32P-labeled ATP led to marked incorporation of radioactivity in wild-type Akt but not Akt/T72A/S246A mutant.	gamma-32p	109-118	AKT serine/threonine kinase 1	Homo sapiens	14-17
246033-3	1978	Direct analysis of the methionyl-tRNAf.40 S complex formed with with eIF-2 and [8-3H] guanine, [gamma-32P]GTP reveals bound guanine but not gamma-phosphate.	gamma-32p	96-105	eukaryotic translation initiation factor 2 subunit beta	Homo sapiens	69-74
20654219-4	2010	The activity of PKC and the expression level of MERTK gene were measured by counting gamma-32P radio-activity with liquid scintillation and RT-PCR respectively.	gamma-32p	85-94	MER proto-oncogene, tyrosine kinase	Homo sapiens	48-53
29803814-1	2018	Traditional CaMKII activity assays rely on incorporation of radioactivity onto a CaMKII substrate by utilizing gamma-32P ATP, which has a short half-life and can pose health risks to the researchers.	gamma-32p	111-120	calcium/calmodulin-dependent protein kinase II, beta	Mus musculus	12-18
29803814-1	2018	Traditional CaMKII activity assays rely on incorporation of radioactivity onto a CaMKII substrate by utilizing gamma-32P ATP, which has a short half-life and can pose health risks to the researchers.	gamma-32p	111-120	calcium/calmodulin-dependent protein kinase II, beta	Mus musculus	81-87
28293845-6	2017	Here, we describe protocols for determining the kinase activity of CTR1 by calculating the incorporated radiolabeled phosphate [gamma-32P] from ATP into its physiological substrate, EIN2 protein.	gamma-32p	128-137	Protein kinase superfamily protein	Arabidopsis thaliana	67-71
28293845-6	2017	Here, we describe protocols for determining the kinase activity of CTR1 by calculating the incorporated radiolabeled phosphate [gamma-32P] from ATP into its physiological substrate, EIN2 protein.	gamma-32p	128-137	NRAMP metal ion transporter family protein	Arabidopsis thaliana	182-186
15730887-10	2005	The total, calcium-dependent and -independent PKC activities were determined by the radioactivity of total gamma-32P transferred to histone.	gamma-32p	107-116	protein kinase C, gamma	Rattus norvegicus	46-49
14725900-10	2004	In addition, [gamma-32P]-labeled TPO-RARE probe bound to KM101 nuclear protein extract was supershifted by anti-RARalpha antibody and modified by treatment with ATRA.	gamma-32p	14-23	thrombopoietin	Homo sapiens	33-36
14725900-10	2004	In addition, [gamma-32P]-labeled TPO-RARE probe bound to KM101 nuclear protein extract was supershifted by anti-RARalpha antibody and modified by treatment with ATRA.	gamma-32p	14-23	retinoic acid receptor alpha	Homo sapiens	112-120
10504387-2	1999	In this study the peptide sequences within or near to the terminal phosphate-binding domains of the GTP and ADP binding sites of bovine liver glutamate dehydrogenase (GDH) were identified using photoaffinity labeling with the benzophenone nucleotide derivatives, [gamma-32P]GTPgammaBP and [gamma-32P]ATPgammaBP.	gamma-32p	264-273	glutamate dehydrogenase 1, mitochondrial	Bos taurus	142-165
12460757-5	2002	The GST-fusion protein was tested for its efficacy and specificity as a substrate for CaMKII in phosphorylation assays using recombinant enzyme and radiolabeled [gamma-32P]ATP.	gamma-32p	162-171	calcium/calmodulin dependent protein kinase II gamma	Homo sapiens	86-92
12845752-4	2000	The expression of MCP-1 mRNA in SMCs was detected by using dot blotting analysis using a probe of gamma-32P-end-labelled 35-mer oligonucleotide.	gamma-32p	98-107	C-C motif chemokine 2	Bos taurus	18-23
10504387-2	1999	In this study the peptide sequences within or near to the terminal phosphate-binding domains of the GTP and ADP binding sites of bovine liver glutamate dehydrogenase (GDH) were identified using photoaffinity labeling with the benzophenone nucleotide derivatives, [gamma-32P]GTPgammaBP and [gamma-32P]ATPgammaBP.	gamma-32p	264-273	glutamate dehydrogenase 1, mitochondrial	Bos taurus	167-170
11715462-3	1999	The MCP-1 mRNA expression in ECs was examined by Dot blot analysis using a gamma-32P-end-labeled 35 mer oligonucleotide probe of MCP-1.	gamma-32p	75-84	C-C motif chemokine ligand 2	Homo sapiens	4-9