PMID-sentid Pub_year Sent_text compound_name comp_offset prot_official_name organism prot_offset 20826087-3 2010 The hydroxychalcones, 2",5"-dihydroxychalcone (D-601) and 2,2"-dihydroxychalcone (D-501), were found to activate heat shock factor 1 (Hsf1) and exhibited radiation sensitization properties in colon and pancreatic cancer cells. 2',5'-dihydroxychalcone 22-45 heat shock transcription factor 1 Homo sapiens 113-132 31633688-2 2019 Therefore, we present here a mechanics-based protocol to probe the influence of gap junction connexin 43 (Cx43) has on endothelial biomechanics by exposing confluent endothelial monolayers to a known Cx43 inhibitor 2,5-dihydroxychalcone (chalcone) and measuring the impact this inhibitor has on tractions and intercellular stresses. 2',5'-dihydroxychalcone 215-236 gap junction protein alpha 1 Homo sapiens 93-104 31633688-2 2019 Therefore, we present here a mechanics-based protocol to probe the influence of gap junction connexin 43 (Cx43) has on endothelial biomechanics by exposing confluent endothelial monolayers to a known Cx43 inhibitor 2,5-dihydroxychalcone (chalcone) and measuring the impact this inhibitor has on tractions and intercellular stresses. 2',5'-dihydroxychalcone 215-236 gap junction protein alpha 1 Homo sapiens 106-110 31633688-2 2019 Therefore, we present here a mechanics-based protocol to probe the influence of gap junction connexin 43 (Cx43) has on endothelial biomechanics by exposing confluent endothelial monolayers to a known Cx43 inhibitor 2,5-dihydroxychalcone (chalcone) and measuring the impact this inhibitor has on tractions and intercellular stresses. 2',5'-dihydroxychalcone 215-236 gap junction protein alpha 1 Homo sapiens 200-204 31543522-3 2019 Therefore, to bring clarity to this relationship we disrupted expression of the endothelial gap junction connexin 43 (Cx43) by exposing confluent human umbilical vein endothelial cells (HUVECs) to a low (0.2 mug/mL) and high (2 mug/mL) concentration of 2,5-dihydroxychalcone (chalcone), a known Cx43 inhibitor. 2',5'-dihydroxychalcone 253-274 gap junction protein alpha 1 Homo sapiens 105-116 31543522-3 2019 Therefore, to bring clarity to this relationship we disrupted expression of the endothelial gap junction connexin 43 (Cx43) by exposing confluent human umbilical vein endothelial cells (HUVECs) to a low (0.2 mug/mL) and high (2 mug/mL) concentration of 2,5-dihydroxychalcone (chalcone), a known Cx43 inhibitor. 2',5'-dihydroxychalcone 253-274 gap junction protein alpha 1 Homo sapiens 118-122 21712085-5 2011 2",5"-DHC treatment induced phosphorylation of the c-Jun N-terminal kinase (JNK) pathway, which was also inhibited by MnTDE-1,3-IP(5+). 2',5'-dihydroxychalcone 0-9 mitogen-activated protein kinase 8 Homo sapiens 51-74 21712085-5 2011 2",5"-DHC treatment induced phosphorylation of the c-Jun N-terminal kinase (JNK) pathway, which was also inhibited by MnTDE-1,3-IP(5+). 2',5'-dihydroxychalcone 0-9 mitogen-activated protein kinase 8 Homo sapiens 76-79 21712085-7 2011 However, whereas 2",5"-DHC triggered the NF-E2-related factor 2 (Nrf2) transcriptional response, cotreatment with MnTDE-1,3-IP(5+) did not decrease 2",5"-DHC-induced Nrf2/ARE activity, showing that this pathway is not dependent on ROS. 2',5'-dihydroxychalcone 17-26 NFE2 like bZIP transcription factor 2 Homo sapiens 65-69 21712085-8 2011 Moreover, pharmacological inhibitors of mitogen-activated protein kinase (MAPK) pathways showed a role for JNK and p38MAPK in mediating the 2",5"-DHC-induced Nrf2 response. 2',5'-dihydroxychalcone 140-149 mitogen-activated protein kinase 8 Homo sapiens 107-110 21712085-8 2011 Moreover, pharmacological inhibitors of mitogen-activated protein kinase (MAPK) pathways showed a role for JNK and p38MAPK in mediating the 2",5"-DHC-induced Nrf2 response. 2',5'-dihydroxychalcone 140-149 NFE2 like bZIP transcription factor 2 Homo sapiens 158-162 20826087-3 2010 The hydroxychalcones, 2",5"-dihydroxychalcone (D-601) and 2,2"-dihydroxychalcone (D-501), were found to activate heat shock factor 1 (Hsf1) and exhibited radiation sensitization properties in colon and pancreatic cancer cells. 2',5'-dihydroxychalcone 22-45 heat shock transcription factor 1 Homo sapiens 134-138 12204542-0 2002 2",5"-Dihydroxychalcone down-regulates endothelial connexin43 gap junctions and affects MAP kinase activation. 2',5'-dihydroxychalcone 0-23 gap junction protein alpha 1 Homo sapiens 51-61 20332504-3 2010 Human epithelial MDA1586, A549, H1975, H460, HN4, and H157 cell lines were exposed to 2",5"-dihydroxychalcone, which induces a GSH efflux response. 2',5'-dihydroxychalcone 86-109 MT-RNR2 like 4 (pseudogene) Homo sapiens 45-48 20332504-5 2010 ABCG2 was identified as the only gene in the array that closely corresponded with the magnitude of 2",5"-dihydroxychalcone (2",5"-DHC)-induced GSH efflux. 2',5'-dihydroxychalcone 99-122 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 0-5 20332504-5 2010 ABCG2 was identified as the only gene in the array that closely corresponded with the magnitude of 2",5"-dihydroxychalcone (2",5"-DHC)-induced GSH efflux. 2',5'-dihydroxychalcone 124-133 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 0-5 20332504-9 2010 In contrast, 2",5"-DHC treatment of ABCG2-expressing yeast increased extracellular GSH levels in a dose-dependent manner with a maximum 3.5-fold increase in GSH after 24 h. In addition, suppression of ABCG2 with short hairpin RNA or ABCG2 overexpression in human epithelial cells decreased or increased extracellular GSH levels, respectively. 2',5'-dihydroxychalcone 13-22 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 36-41 20332504-9 2010 In contrast, 2",5"-DHC treatment of ABCG2-expressing yeast increased extracellular GSH levels in a dose-dependent manner with a maximum 3.5-fold increase in GSH after 24 h. In addition, suppression of ABCG2 with short hairpin RNA or ABCG2 overexpression in human epithelial cells decreased or increased extracellular GSH levels, respectively. 2',5'-dihydroxychalcone 13-22 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 201-206 20332504-9 2010 In contrast, 2",5"-DHC treatment of ABCG2-expressing yeast increased extracellular GSH levels in a dose-dependent manner with a maximum 3.5-fold increase in GSH after 24 h. In addition, suppression of ABCG2 with short hairpin RNA or ABCG2 overexpression in human epithelial cells decreased or increased extracellular GSH levels, respectively. 2',5'-dihydroxychalcone 13-22 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 201-206 12204542-1 2002 We examined the effect of 2",5"-dihydroxychalcone on connexin43 (Cx43) expression and gap-junctional communication in human umbilical vein endothelial cells (HUVEC). 2',5'-dihydroxychalcone 26-49 gap junction protein alpha 1 Homo sapiens 53-63 12204542-1 2002 We examined the effect of 2",5"-dihydroxychalcone on connexin43 (Cx43) expression and gap-junctional communication in human umbilical vein endothelial cells (HUVEC). 2',5'-dihydroxychalcone 26-49 gap junction protein alpha 1 Homo sapiens 65-69 12204542-2 2002 The result showed that expression of Cx43 is rapidly reduced by 2",5"-dihydroxychalcone in a dose-dependent manner, Concomitantly, the communication function, determined by fluorescence recovery after photobleaching (FRAP), is decreased. 2',5'-dihydroxychalcone 64-87 gap junction protein alpha 1 Homo sapiens 37-41 12204542-7 2002 On the other hand, the chalcone"s down-regulating effect on Cx43, while is totally blocked by protease inhibitors leupeptin and N-acetyl-leucyl-norleucinal (ALLN), persists in the presence of PD98059, We concluded that 2",5"-dihydroxychalcone down-regulates Cx43 expression and gap-junctional communication in the HUVEC via enhancement of the proteolysis pathway, and this compound possesses dual effects on MAP kinase activation. 2',5'-dihydroxychalcone 219-242 gap junction protein alpha 1 Homo sapiens 60-64