PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 19699525-11 2009 Interestingly, IL-8 mRNA was induced by cycloheximide treatment and RANTES showed reduced mRNA but increased protein expression by antibody stimulation. Cycloheximide 40-53 C-X-C motif chemokine ligand 8 Homo sapiens 15-19 28323848-4 2017 7alphaOHChol-induced IL-8 gene transcription was inhibited by cycloheximide and Akt1 downregulation, but not by OxPAPC. Cycloheximide 62-75 C-X-C motif chemokine ligand 8 Homo sapiens 21-25 26086093-4 2015 27OHChol-induced transcription of the IL-8 gene was blocked by cycloheximide, but not by polymyxin B. Cycloheximide 63-76 C-X-C motif chemokine ligand 8 Homo sapiens 38-42 17606477-3 2007 Administration of recombinant human IL-8 induced a rapid, time-dependent increase in cyclin D1 expression in AIPC cells, a response attenuated by the translation inhibitor cycloheximide but not by the RNA synthesis inhibitor, actinomycin D. Cycloheximide 172-185 C-X-C motif chemokine ligand 8 Homo sapiens 36-40 19376214-4 2009 NaF-induced accumulation of COX-2 transcript was abolished by actinomycin D, but not cycloheximide. Cycloheximide 85-98 C-X-C motif chemokine ligand 8 Homo sapiens 0-3 18729741-4 2008 Cycloheximide treatment indicated that the augmenting effect of CSC on IL-1alpha, IL-1beta and IL-8, but not IL-6 and CYP1A1, mRNA expression requires de novo protein synthesis. Cycloheximide 0-13 C-X-C motif chemokine ligand 8 Homo sapiens 95-99 17671691-8 2007 Actinomycin D suppressed and cycloheximide augmented CXCL-8 mRNA which was induced by TNF-alpha or not. Cycloheximide 29-42 C-X-C motif chemokine ligand 8 Homo sapiens 53-59 16085794-7 2005 The augmented IL-8 mRNA expression in cord blood was inhibited by actinomycin D but enhanced by cycloheximide, suggesting that IL-8 production is controlled by de novo transcriptional induction as well as posttranscriptional up-regulation of IL-8 by neonatal leukocytes, relating to the development of CLD. Cycloheximide 96-109 C-X-C motif chemokine ligand 8 Homo sapiens 14-18 17286758-5 2007 Cycloheximide reduced the UVB-mediated induction of IL-8 by 30-40%, suggesting that new protein synthesis contributed to IL-8 production. Cycloheximide 0-13 C-X-C motif chemokine ligand 8 Homo sapiens 52-56 17286758-5 2007 Cycloheximide reduced the UVB-mediated induction of IL-8 by 30-40%, suggesting that new protein synthesis contributed to IL-8 production. Cycloheximide 0-13 C-X-C motif chemokine ligand 8 Homo sapiens 121-125 16541418-8 2006 TGF-beta1 stimulated IL-8 expression in dose- and time-dependent manners, which was blocked by cycloheximide (CHX) and actinomycin D (ActD). Cycloheximide 95-108 C-X-C motif chemokine ligand 8 Homo sapiens 21-25 16541418-8 2006 TGF-beta1 stimulated IL-8 expression in dose- and time-dependent manners, which was blocked by cycloheximide (CHX) and actinomycin D (ActD). Cycloheximide 110-113 C-X-C motif chemokine ligand 8 Homo sapiens 21-25 16479515-7 2006 CXCL-8 mRNA was superinduced by TNF- alpha in the presence of the protein-synthesis inhibitor cycloheximide. Cycloheximide 94-107 C-X-C motif chemokine ligand 8 Homo sapiens 0-6 16085794-7 2005 The augmented IL-8 mRNA expression in cord blood was inhibited by actinomycin D but enhanced by cycloheximide, suggesting that IL-8 production is controlled by de novo transcriptional induction as well as posttranscriptional up-regulation of IL-8 by neonatal leukocytes, relating to the development of CLD. Cycloheximide 96-109 C-X-C motif chemokine ligand 8 Homo sapiens 127-131 16085794-7 2005 The augmented IL-8 mRNA expression in cord blood was inhibited by actinomycin D but enhanced by cycloheximide, suggesting that IL-8 production is controlled by de novo transcriptional induction as well as posttranscriptional up-regulation of IL-8 by neonatal leukocytes, relating to the development of CLD. Cycloheximide 96-109 C-X-C motif chemokine ligand 8 Homo sapiens 127-131 11531942-7 2001 IL-8 mRNA accumulation was detected by Northern blot analysis within 2 h of stimulation by the immune complexes and was enhanced by the addition of cycloheximide. Cycloheximide 148-161 C-X-C motif chemokine ligand 8 Homo sapiens 0-4 15498828-6 2005 IL-8 release was blocked by actinomycin D or cycloheximide, but the inhibitors had no effect on VEGF release, suggesting that IL-8 secretion required de novo synthesis whereas VEGF was secreted from preformed stores. Cycloheximide 45-58 C-X-C motif chemokine ligand 8 Homo sapiens 0-4 15181190-2 2004 We have shown that recombinant human IL-8/CXCL8 (rhIL-8/CXCL8) protects cultured IEC against tumor necrosis factor (TNF)-alpha and cycloheximide-induced cytotoxicity. Cycloheximide 131-144 C-X-C motif chemokine ligand 8 Homo sapiens 37-41 15181190-2 2004 We have shown that recombinant human IL-8/CXCL8 (rhIL-8/CXCL8) protects cultured IEC against tumor necrosis factor (TNF)-alpha and cycloheximide-induced cytotoxicity. Cycloheximide 131-144 C-X-C motif chemokine ligand 8 Homo sapiens 42-47 15181190-2 2004 We have shown that recombinant human IL-8/CXCL8 (rhIL-8/CXCL8) protects cultured IEC against tumor necrosis factor (TNF)-alpha and cycloheximide-induced cytotoxicity. Cycloheximide 131-144 C-X-C motif chemokine ligand 8 Homo sapiens 56-61 12683216-4 2002 Based on the inhibitory effect of cycloheximide, actinomycin-D we may suggest that PMA and LPS could upregulate IL-8 receptor in monocytes through denovo protein synthesis. Cycloheximide 34-47 C-X-C motif chemokine ligand 8 Homo sapiens 112-116 11076803-3 2000 Sepharose-immobilized IL-8 stimulated a sevenfold increase in IL-8 production within 2 h. IL-8 induced the expression of its own message, and IL-8 biosynthesis was inhibited by cycloheximide and actinomycin D, indicating de novo RNA and protein synthesis. Cycloheximide 177-190 C-X-C motif chemokine ligand 8 Homo sapiens 22-26 11076803-3 2000 Sepharose-immobilized IL-8 stimulated a sevenfold increase in IL-8 production within 2 h. IL-8 induced the expression of its own message, and IL-8 biosynthesis was inhibited by cycloheximide and actinomycin D, indicating de novo RNA and protein synthesis. Cycloheximide 177-190 C-X-C motif chemokine ligand 8 Homo sapiens 62-66 11076803-3 2000 Sepharose-immobilized IL-8 stimulated a sevenfold increase in IL-8 production within 2 h. IL-8 induced the expression of its own message, and IL-8 biosynthesis was inhibited by cycloheximide and actinomycin D, indicating de novo RNA and protein synthesis. Cycloheximide 177-190 C-X-C motif chemokine ligand 8 Homo sapiens 62-66 11076803-3 2000 Sepharose-immobilized IL-8 stimulated a sevenfold increase in IL-8 production within 2 h. IL-8 induced the expression of its own message, and IL-8 biosynthesis was inhibited by cycloheximide and actinomycin D, indicating de novo RNA and protein synthesis. Cycloheximide 177-190 C-X-C motif chemokine ligand 8 Homo sapiens 62-66 10979965-8 2000 Secretion of IL-8 reached a plateau level in less than 24 hours, was inhibited by cycloheximide, and required the presence of HrHRF throughout the culture period. Cycloheximide 82-95 C-X-C motif chemokine ligand 8 Homo sapiens 13-17 10602388-6 1999 Time-course experiments showed a NaF-induced IL-6 response at 5 h, whereas an IL-8 response was observed after 10 h. Cycloheximide treatment completely abolished the NaF-induced cytokine responses. Cycloheximide 117-130 C-X-C motif chemokine ligand 8 Homo sapiens 78-82 10964761-6 2000 Cotreatment with PDTC and curcumin reduced particle-elicited IL-8 response, whereas cycloheximide caused enhancement of IL-8 mRNA expression in both the quartz- and TiO(2)-treated cells. Cycloheximide 84-97 C-X-C motif chemokine ligand 8 Homo sapiens 120-124 10583443-12 1999 Anti-PR-3 antibody induced endothelial IL-8 expression could be inhibited by cycloheximide. Cycloheximide 77-90 C-X-C motif chemokine ligand 8 Homo sapiens 39-43 9780210-2 1998 Incubation of peripheral blood neutrophils with thapsigargin, an inhibitor of the endoplasmic reticulum Ca2+-sequestering-ATPase, causes a dose-dependent induction of IL-8 synthesis that continues for up to 8 h. Cycloheximide inhibits the thapsigargin-induced IL-8 production, suggesting the induction of protein synthesis de novo. Cycloheximide 212-225 C-X-C motif chemokine ligand 8 Homo sapiens 167-171 9916736-5 1999 While actinomycin-D (1 microg/ml) had little influence on the generation of IL-8 during chemotaxis, the protein synthesis inhibitor cycloheximide (10 microg/ml) markedly blunted the accumulation of cell-associated IL-8, suggesting that new protein synthesis from preexisting mRNA was responsible for the effect. Cycloheximide 132-145 C-X-C motif chemokine ligand 8 Homo sapiens 214-218 10484459-3 1999 We now show that E1A-dependent induction of interleukin-8 expression is specific to LPS, superinduced by cycloheximide, and not observed after tumor necrosis factor or phorbol 12-myristate 13-acetate stimulation. Cycloheximide 105-118 C-X-C motif chemokine ligand 8 Homo sapiens 44-57 9136832-8 1997 Cycloheximide treatment resulted in superinduction of IL-8 mRNA; however, dexamethasone inhibited E. histolytica-induced IL-8 gene expression. Cycloheximide 0-13 C-X-C motif chemokine ligand 8 Homo sapiens 54-58 9461540-4 1998 Cycloheximide (CHI, 10 microg/ml), an inhibitor of protein synthesis, maximally increased IL-8 mRNA levels 30-fold in H292 cells. Cycloheximide 0-13 C-X-C motif chemokine ligand 8 Homo sapiens 90-94 9461540-4 1998 Cycloheximide (CHI, 10 microg/ml), an inhibitor of protein synthesis, maximally increased IL-8 mRNA levels 30-fold in H292 cells. Cycloheximide 15-18 C-X-C motif chemokine ligand 8 Homo sapiens 90-94 9725250-11 1998 Both the BK- and arachidonic acid-induced IL-8 production was inhibited by the protein synthesis inhibitors cycloheximide and actinomycin D and by the steroid dexamethasone. Cycloheximide 108-121 C-X-C motif chemokine ligand 8 Homo sapiens 42-46 8886420-14 1996 Experiments using cycloheximide demonstrated that this synergistic effect of IL-13 and IL-1 alpha on IL-8 secretion was not through de novo protein synthesis. Cycloheximide 18-31 C-X-C motif chemokine ligand 8 Homo sapiens 101-105 9081687-6 1997 This stimulation of IL-8 production was time and dose dependent and could be blocked by cycloheximide. Cycloheximide 88-101 C-X-C motif chemokine ligand 8 Homo sapiens 20-24 9020026-3 1997 The time-dependent accumulation of IL-8 inhibited by cycloheximide and the evaluation of the IL-8 mRNA levels by reverse transcriptase-polymerase chain reaction suggested that its action occurred in the posttranscriptional processes. Cycloheximide 53-66 C-X-C motif chemokine ligand 8 Homo sapiens 35-39 8985363-6 1997 Moreover, interleukin-8 (IL-8) mRNA accumulation was evident at 20 min postinfection and was induced even in the presence of cycloheximide. Cycloheximide 125-138 C-X-C motif chemokine ligand 8 Homo sapiens 10-23 8985363-6 1997 Moreover, interleukin-8 (IL-8) mRNA accumulation was evident at 20 min postinfection and was induced even in the presence of cycloheximide. Cycloheximide 125-138 C-X-C motif chemokine ligand 8 Homo sapiens 25-29 8877727-3 1996 This enhanced expression of IL-8 was inhibited by cycloheximide or actinomycin-D. Cycloheximide 50-63 C-X-C motif chemokine ligand 8 Homo sapiens 28-32 8830798-6 1996 The GHSA-induced chemokine secretion by hRPE was almost completely inhibited by actinomycin D and cycloheximide, suggesting that de novo mRNA and protein synthesis are necessary for the GHSA-induced IL-8 and MCP-1 production. Cycloheximide 98-111 C-X-C motif chemokine ligand 8 Homo sapiens 199-203 8004813-4 1994 In contrast, IL-10 was weakly expressed when fibroblasts were stimulated with LPS, IL-1 alpha or tumour necrosis factor-alpha (TNF-alpha), but the expression was enhanced in the presence of cycloheximide combined with optimal concentrations of LPS, IL-1 alpha or TNF-alpha, IL-1 alpha was a more potent stimulator than LPS for GM-CSF, IL-6, IL-8 and IL-10 expression, but not for IL-1 alpha and IL-1 beta. Cycloheximide 190-203 C-X-C motif chemokine ligand 8 Homo sapiens 341-345 8738804-8 1996 Both proteinase 3-and elastase-mediated production of IL-8 was inhibited by cycloheximide, indicating de novo synthesis. Cycloheximide 76-89 C-X-C motif chemokine ligand 8 Homo sapiens 54-58 7759889-8 1995 The accumulation of IL-8 within these organelles is inhibited by cycloheximide but not actinomycin D, suggesting that IL-8 accumulation is under translational, rather than transcriptional control. Cycloheximide 65-78 C-X-C motif chemokine ligand 8 Homo sapiens 20-24 7759889-8 1995 The accumulation of IL-8 within these organelles is inhibited by cycloheximide but not actinomycin D, suggesting that IL-8 accumulation is under translational, rather than transcriptional control. Cycloheximide 65-78 C-X-C motif chemokine ligand 8 Homo sapiens 118-122 7943343-4 1994 TNF-alpha-induced IL-8 mRNA expression showed a biphasic response in HAEC, with an early increase at 2 h followed by a sustained increase from 8 h, which was abolished by the addition of cycloheximide, suggesting that the synthesis of another protein was involved. Cycloheximide 187-200 C-X-C motif chemokine ligand 8 Homo sapiens 18-22 7637390-3 1995 However, the rate of transient induction kinetics was modulated by cycloheximide (CHX) indicating that secondary response genes are involved in the regulation of IL-8 RNA levels. Cycloheximide 67-80 C-X-C motif chemokine ligand 8 Homo sapiens 162-166 7637390-3 1995 However, the rate of transient induction kinetics was modulated by cycloheximide (CHX) indicating that secondary response genes are involved in the regulation of IL-8 RNA levels. Cycloheximide 82-85 C-X-C motif chemokine ligand 8 Homo sapiens 162-166 8262617-6 1994 The expression of IL-8 mRNA from LTA- but not lipopolysaccharide (LPS)-treated PBM was superinduced by concomitant treatment with cycloheximide, indicating that the expression of IL-8 mRNA from LTA-treated PBM was negatively controlled by repressor proteins. Cycloheximide 130-143 C-X-C motif chemokine ligand 8 Homo sapiens 18-22 8073837-7 1994 IL-8 and huGRO mRNAs were constitutively produced in KC, as was demonstrated after incubation with cycloheximide. Cycloheximide 99-112 C-X-C motif chemokine ligand 8 Homo sapiens 0-4 7514637-2 1994 Zymosan stimulated IL-8 production, which increased with increasing particle numbers and was abolished by the protein synthesis inhibitor cycloheximide. Cycloheximide 138-151 C-X-C motif chemokine ligand 8 Homo sapiens 19-23 8144938-4 1994 The protein synthesis inhibitor, cycloheximide, had no detectable effect on levels of IL-8 mRNA expression in PMN incubated in medium alone; however, cycloheximide could selectively modulate IL-8 mRNA transcription in PMN, depending on the cytokine used. Cycloheximide 33-46 C-X-C motif chemokine ligand 8 Homo sapiens 191-195 8144938-4 1994 The protein synthesis inhibitor, cycloheximide, had no detectable effect on levels of IL-8 mRNA expression in PMN incubated in medium alone; however, cycloheximide could selectively modulate IL-8 mRNA transcription in PMN, depending on the cytokine used. Cycloheximide 150-163 C-X-C motif chemokine ligand 8 Homo sapiens 191-195 8144938-5 1994 Cycloheximide did not affect or alter IL-8 mRNA induction in IL-2-treated PMN but abrogated it in granulocyte macrophage-CSF-treated PMN and super-induced the level of IL-8 mRNA in C. albicans-treated PMN. Cycloheximide 0-13 C-X-C motif chemokine ligand 8 Homo sapiens 168-172 8262617-6 1994 The expression of IL-8 mRNA from LTA- but not lipopolysaccharide (LPS)-treated PBM was superinduced by concomitant treatment with cycloheximide, indicating that the expression of IL-8 mRNA from LTA-treated PBM was negatively controlled by repressor proteins. Cycloheximide 130-143 C-X-C motif chemokine ligand 8 Homo sapiens 179-183 8254194-11 1994 However, treatment of monocytes with cycloheximide resulted in the superinduction of IL-8 compared with control monocytes. Cycloheximide 37-50 C-X-C motif chemokine ligand 8 Homo sapiens 85-89 8228330-13 1993 Cycloheximide treatment blocked this IL-8 protein induction. Cycloheximide 0-13 C-X-C motif chemokine ligand 8 Homo sapiens 37-41 8458381-4 1993 Furthermore, eosinophil production of IL-8 in the presence of calcium ionophore could be inhibited with the immunomodulating agent cyclosporin A and the protein synthesis inhibitor cycloheximide. Cycloheximide 181-194 C-X-C motif chemokine ligand 8 Homo sapiens 38-42 8496597-0 1993 Differential action of cycloheximide and activation stimuli on transcription of tumor necrosis factor-alpha, IL-1 beta, IL-8, and P53 genes in human monocytes. Cycloheximide 23-36 C-X-C motif chemokine ligand 8 Homo sapiens 120-124 7680648-5 1993 Preincubation of the cells with cycloheximide "superinduced" the level of IL-8 mRNA stimulated by TNF alpha and IL-1 beta and RANTES mRNA stimulated by IL-1 beta, but decreased the expression of RANTES mRNA in response to TNF alpha. Cycloheximide 32-45 C-X-C motif chemokine ligand 8 Homo sapiens 74-78 8432783-6 1993 Cotreatment of chorion cells or decidual cells with IL-1 beta and actinomycin D or cycloheximide abrogated IL-8 production. Cycloheximide 83-96 C-X-C motif chemokine ligand 8 Homo sapiens 107-111 8473010-7 1993 Further observations revealed that, in human PMN, degradation of IL-8 mRNA is finely regulated, and that cycloheximide (CHX), an inhibitor of protein synthesis, super-induces the mRNA accumulation for IL-8 in a dose- and time-dependent manner. Cycloheximide 105-118 C-X-C motif chemokine ligand 8 Homo sapiens 201-205 8473010-7 1993 Further observations revealed that, in human PMN, degradation of IL-8 mRNA is finely regulated, and that cycloheximide (CHX), an inhibitor of protein synthesis, super-induces the mRNA accumulation for IL-8 in a dose- and time-dependent manner. Cycloheximide 120-123 C-X-C motif chemokine ligand 8 Homo sapiens 65-69 8473010-7 1993 Further observations revealed that, in human PMN, degradation of IL-8 mRNA is finely regulated, and that cycloheximide (CHX), an inhibitor of protein synthesis, super-induces the mRNA accumulation for IL-8 in a dose- and time-dependent manner. Cycloheximide 120-123 C-X-C motif chemokine ligand 8 Homo sapiens 201-205 1497091-6 1992 In addition, when neutrophils were treated with cycloheximide, there was evidence for "superinduction" of steady-state levels of IL-8 mRNA and inhibition of antigenic IL-8 production. Cycloheximide 48-61 C-X-C motif chemokine ligand 8 Homo sapiens 129-133 1497091-6 1992 In addition, when neutrophils were treated with cycloheximide, there was evidence for "superinduction" of steady-state levels of IL-8 mRNA and inhibition of antigenic IL-8 production. Cycloheximide 48-61 C-X-C motif chemokine ligand 8 Homo sapiens 167-171 1856599-6 1991 Antigenic and bioactive IL-8 were significantly apparent by 4-8 h, respectively, and increased significantly to maximal levels by 24 h. Furthermore, adherent PBMC IL-8 gene expression was suppressed by either concomitant treatment with actinomycin-D or cycloheximide, yet specific neutralizing antibodies directed against either IL-1 beta or tumor necrosis factor (TNF)-alpha failed to alter adherence-induced steady-state IL-8 mRNA levels. Cycloheximide 253-266 C-X-C motif chemokine ligand 8 Homo sapiens 24-28 1856599-6 1991 Antigenic and bioactive IL-8 were significantly apparent by 4-8 h, respectively, and increased significantly to maximal levels by 24 h. Furthermore, adherent PBMC IL-8 gene expression was suppressed by either concomitant treatment with actinomycin-D or cycloheximide, yet specific neutralizing antibodies directed against either IL-1 beta or tumor necrosis factor (TNF)-alpha failed to alter adherence-induced steady-state IL-8 mRNA levels. Cycloheximide 253-266 C-X-C motif chemokine ligand 8 Homo sapiens 163-167 1856599-6 1991 Antigenic and bioactive IL-8 were significantly apparent by 4-8 h, respectively, and increased significantly to maximal levels by 24 h. Furthermore, adherent PBMC IL-8 gene expression was suppressed by either concomitant treatment with actinomycin-D or cycloheximide, yet specific neutralizing antibodies directed against either IL-1 beta or tumor necrosis factor (TNF)-alpha failed to alter adherence-induced steady-state IL-8 mRNA levels. Cycloheximide 253-266 C-X-C motif chemokine ligand 8 Homo sapiens 163-167 2200823-7 1990 The IL-4-induced inhibition of IL-8 mRNA expression was dependent on protein synthesis, as the suppressive effects of IL-4 were significantly negated by the addition of cycloheximide. Cycloheximide 169-182 C-X-C motif chemokine ligand 8 Homo sapiens 31-35 1401924-6 1992 Maximum accumulation of IL-8 mRNA was observed after 6 h of incubation with LR, and the elevation persisted over 24 h. Inhibition of protein synthesis by cycloheximide resulted in superinduction of IL-8 mRNAs by LR. Cycloheximide 154-167 C-X-C motif chemokine ligand 8 Homo sapiens 24-28 1401924-6 1992 Maximum accumulation of IL-8 mRNA was observed after 6 h of incubation with LR, and the elevation persisted over 24 h. Inhibition of protein synthesis by cycloheximide resulted in superinduction of IL-8 mRNAs by LR. Cycloheximide 154-167 C-X-C motif chemokine ligand 8 Homo sapiens 198-202 1522134-6 1992 Treatment of amnion cells stimulated by IL-1 beta with cycloheximide resulted in increased IL-8 production, while incubation of IL-1 beta treated amnion cells with actinomycin D resulted in a concentration-dependent decrease in detectable amounts of IL-8. Cycloheximide 55-68 C-X-C motif chemokine ligand 8 Homo sapiens 91-95 1522134-6 1992 Treatment of amnion cells stimulated by IL-1 beta with cycloheximide resulted in increased IL-8 production, while incubation of IL-1 beta treated amnion cells with actinomycin D resulted in a concentration-dependent decrease in detectable amounts of IL-8. Cycloheximide 55-68 C-X-C motif chemokine ligand 8 Homo sapiens 250-254 1639472-4 1992 IL-8 is detected by enzyme-linked immunosorbent assay within 2 h of stimulation, with steady a increase in its level through 72 h. Further studies demonstrated that LPS could serve as a primary stimulus for the expression of IL-8, since LPS challenge in the presence of cycloheximide resulted in superinduction of bone marrow mononuclear cell-derived IL-8 mRNA. Cycloheximide 270-283 C-X-C motif chemokine ligand 8 Homo sapiens 0-4 1639472-4 1992 IL-8 is detected by enzyme-linked immunosorbent assay within 2 h of stimulation, with steady a increase in its level through 72 h. Further studies demonstrated that LPS could serve as a primary stimulus for the expression of IL-8, since LPS challenge in the presence of cycloheximide resulted in superinduction of bone marrow mononuclear cell-derived IL-8 mRNA. Cycloheximide 270-283 C-X-C motif chemokine ligand 8 Homo sapiens 225-229 1639472-4 1992 IL-8 is detected by enzyme-linked immunosorbent assay within 2 h of stimulation, with steady a increase in its level through 72 h. Further studies demonstrated that LPS could serve as a primary stimulus for the expression of IL-8, since LPS challenge in the presence of cycloheximide resulted in superinduction of bone marrow mononuclear cell-derived IL-8 mRNA. Cycloheximide 270-283 C-X-C motif chemokine ligand 8 Homo sapiens 225-229 1578146-6 1992 IL-8 release was dependent on FMLP-induced de novo protein synthesis because it was inhibited by cycloheximide, was paralleled by enhanced expression of IL-8 mRNA and was potentiated from two- to sixfold after preincubation of PMN with cytochalasin B. Cycloheximide 97-110 C-X-C motif chemokine ligand 8 Homo sapiens 0-4