PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 3624250-4 1987 The enhancement of ACAT activity was seen 4 h after the addition of cycloheximide, and ACAT activity returned to control values 4 h after the withdrawal of cycloheximide. Cycloheximide 156-169 acetyl-Coenzyme A acetyltransferase 1 Mus musculus 87-91 3624250-3 1987 We now report that when protein synthesis is inhibited in mouse peritoneal macrophages by treatment with cycloheximide, puromycin, or actinomycin D, native LDL-induced whole-cell ACAT activity and CE accumulation is 10-fold higher than that seen in LDL-treated control cells. Cycloheximide 105-118 acetyl-Coenzyme A acetyltransferase 1 Mus musculus 179-183 3624250-4 1987 The enhancement of ACAT activity was seen 4 h after the addition of cycloheximide, and ACAT activity returned to control values 4 h after the withdrawal of cycloheximide. Cycloheximide 68-81 acetyl-Coenzyme A acetyltransferase 1 Mus musculus 19-23 3624250-5 1987 Postnuclear supernatants and microsomes from cycloheximide-treated mouse peritoneal macrophages also had higher ACAT activity than microsomes from control cells, but the relative enhancement (maximum 3.3-fold) was less than that seen when ACAT was assayed in the intact cell. Cycloheximide 45-58 acetyl-Coenzyme A acetyltransferase 1 Mus musculus 112-116 3624250-5 1987 Postnuclear supernatants and microsomes from cycloheximide-treated mouse peritoneal macrophages also had higher ACAT activity than microsomes from control cells, but the relative enhancement (maximum 3.3-fold) was less than that seen when ACAT was assayed in the intact cell. Cycloheximide 45-58 acetyl-Coenzyme A acetyltransferase 1 Mus musculus 239-243 3624250-6 1987 In contrast to the situation with mouse peritoneal macrophages, cycloheximide treatment of J774 macrophages, which under normal conditions display high ACAT activity and CE accumulation in the presence of native LDL, did not result in further enhancement of either ACAT activity or LDL-induced CE accumulation. Cycloheximide 64-77 acetyl-Coenzyme A acetyltransferase 1 Mus musculus 152-156