PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
10341035-2	1999	In the present study, we explore the possibility that residual nigericin from dye calibration in one experiment might interfere with intracellular pH (pHi) changes in the next.	Nigericin	63-72	glucose-6-phosphate isomerase	Rattus norvegicus	151-154	
10341035-4	1999	Nigericin contamination caused: (i) an increase in acid loading during the pHi decrease elicited by removing extracellular Na+, (ii) an increase in acid extrusion during the pHi increase caused by elevating extracellular [K+], and (iii) an acid shift in the pHi dependence of the background intracellular acid loading unmasked by inhibiting Na-H exchange with ethylisopropylamiloride (EIPA).	Nigericin	0-9	glucose-6-phosphate isomerase	Rattus norvegicus	75-78	
10341035-4	1999	Nigericin contamination caused: (i) an increase in acid loading during the pHi decrease elicited by removing extracellular Na+, (ii) an increase in acid extrusion during the pHi increase caused by elevating extracellular [K+], and (iii) an acid shift in the pHi dependence of the background intracellular acid loading unmasked by inhibiting Na-H exchange with ethylisopropylamiloride (EIPA).	Nigericin	0-9	glucose-6-phosphate isomerase	Rattus norvegicus	174-177	
10341035-4	1999	Nigericin contamination caused: (i) an increase in acid loading during the pHi decrease elicited by removing extracellular Na+, (ii) an increase in acid extrusion during the pHi increase caused by elevating extracellular [K+], and (iii) an acid shift in the pHi dependence of the background intracellular acid loading unmasked by inhibiting Na-H exchange with ethylisopropylamiloride (EIPA).	Nigericin	0-9	glucose-6-phosphate isomerase	Rattus norvegicus	174-177	
8897819-5	1996	This discrepancy became small if pHi had really been approximately 0.2 lower than what was estimated using nigericin-calibrated pHi values.	Nigericin	107-116	glucose-6-phosphate isomerase	Rattus norvegicus	33-36	
9887007-8	1999	Interventions that load the cells with protons without affecting superfusate pH (NH4Cl prepulse, nigericin with low superfusate K+ concentration, DMA, and DIDS) all decreased pHi, supporting our contention that the dye was faithfully measuring pHi.	Nigericin	97-106	glucose-6-phosphate isomerase	Rattus norvegicus	175-178	
8897819-5	1996	This discrepancy became small if pHi had really been approximately 0.2 lower than what was estimated using nigericin-calibrated pHi values.	Nigericin	107-116	glucose-6-phosphate isomerase	Rattus norvegicus	128-131	
8897819-7	1996	If the true pHi values were approximately 0.2-0.4 lower than our nigericin-calibrated values, then the sum of beta int and beta CO2 equals beta tot.	Nigericin	65-74	glucose-6-phosphate isomerase	Rattus norvegicus	12-15	
8897819-11	1996	Finally, one potential source for these discrepancies is described: selecting an inappropriate external K+ to use with nigericin can produce systematic errors in pHi of approximately 0.1.	Nigericin	119-128	glucose-6-phosphate isomerase	Rattus norvegicus	162-165	
3373180-5	1988	When the fluorescence signal was calibrated by permeabilizing the synaptosomal membrane to H+ with gramicidin and nigericin, pHi was estimated to be 7.19 +/- 0.03 (n = 12).	Nigericin	114-123	glucose-6-phosphate isomerase	Rattus norvegicus	125-128	
7789340-2	1995	The resting pHi was acidified from 7.30 +/- 0.02 to 6.99 +/- 0.01 with nigericin in the absence of (Na+)o.	Nigericin	71-80	glucose-6-phosphate isomerase	Rattus norvegicus	12-15	
1637840-3	1992	We found that various experimental manipulations that increased pHi, such as exposure of the cells to NH4Cl, a decrease of the partial pressure of CO2 or an increase in extracellular pH in the presence of nigericin invariably increased Cai.	Nigericin	205-214	glucose-6-phosphate isomerase	Rattus norvegicus	64-67	
1887901-3	1991	An in vivo calibration curve was constructed using 10(-5) M nigericin to equilibrate pHi and extracellular pH, and intra- and extracellular potassium concentrations.	Nigericin	60-69	glucose-6-phosphate isomerase	Rattus norvegicus	85-88	
1887901-7	1991	pHi increased when the mucosa was superfused, in the presence of nigericin, with 150 mM KCl solutions and decreased when superfused with 2.6 mM KCl solutions.	Nigericin	65-74	glucose-6-phosphate isomerase	Rattus norvegicus	0-3	
1961691-4	1991	Intracellular calibration of the dye was performed by clamping the intracellular to the extracellular pH with H+/K(+)-ionophore Nigericin.	Nigericin	128-137	glucose-6-phosphate isomerase	Rattus norvegicus	102-104	
2833308-3	1988	When freshly isolated alveolar type II cells were acid loaded with nigericin in sodium-free buffer, the pHi dropped to 6.59 +/- 0.04 and remained low in sodium-free buffer.	Nigericin	67-76	glucose-6-phosphate isomerase	Rattus norvegicus	104-107	
2833308-8	1988	Nigericin acidified these cultured cells in the absence of sodium and NaCl increased the pHi of these acid loaded cells as observed in freshly isolated cells.	Nigericin	0-9	glucose-6-phosphate isomerase	Rattus norvegicus	89-92	
6325586-2	1984	Cells that were acid-loaded with nigericin in choline+ media recovered normal pHi upon addition of extracellular Na+ (Nao+).	Nigericin	33-42	glucose-6-phosphate isomerase	Rattus norvegicus	78-81