PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 10197642-5 1999 Clamping of pHi at 7.25 by the proton-ionophore nigericin abolished SST-signaled apoptosis without affecting its ability to regulate SHP-1, p53, and Bax. Nigericin 48-57 glucose-6-phosphate isomerase Homo sapiens 12-15 15601755-4 2005 Acidification with the K+/H+ exchanger nigericin reduced pHi to 6.25+/-0.15 (without HCO3-) or 6.53+/-0.10 (with HCO3-). Nigericin 39-48 glucose-6-phosphate isomerase Homo sapiens 57-60 15382148-5 2004 The calibration of fluorescence ratios versus pHi was established by using ionophore nigericin. Nigericin 85-94 glucose-6-phosphate isomerase Homo sapiens 46-49 10404735-4 1999 Sodium/hydrogen exchanger activity was estimated from the recovery of pHi clamped to 6.25 with nigericin. Nigericin 95-104 glucose-6-phosphate isomerase Homo sapiens 70-73 10197642-6 1999 Apoptosis could be induced by nigericin clamping of pHi to 6.5. Nigericin 30-39 glucose-6-phosphate isomerase Homo sapiens 52-55 8895841-2 1996 In the presence of Na+ at a physiological concentration (147 mM), nigericin (0.5 microM) elevates [Nai] from 20 to 50 mM, increases the pHi, 0.16 pH units, elevates four fold the [Cai] at expense of external Ca2+ and markedly increases (more than five fold) the release of [3H]GABA. Nigericin 66-75 glucose-6-phosphate isomerase Homo sapiens 136-139 8987090-15 1996 Similar pH-dependent MMC activity was found when multilayers were pre-treated for 1 hr with 0.5 microgram/ml nigericin, a proton ionophore known to cause the intracellular pH (pHi) to equilibrate with pHe. Nigericin 109-118 glucose-6-phosphate isomerase Homo sapiens 176-179 8931385-2 1996 Nigericin equilibrates intracellular pH (pHi) and extracellular pH (pHe) and is most effective in potentiating PDT damage when cells are in an acidic environment (pH 6.5-6.7). Nigericin 0-9 glucose-6-phosphate isomerase Homo sapiens 41-44 8931385-3 1996 We therefore hypothesized that the ability of nigericin to lower pHi is causally related to its ability to potentiate PDT. Nigericin 46-55 glucose-6-phosphate isomerase Homo sapiens 65-68 8931385-9 1996 In contrast, 20 microM nigericin in medium at pHe 6.7 reduces pHi to 6.55, but reduces the surviving fraction at 20 kJ/m2 by nearly 3 logs relative to controls. Nigericin 23-32 glucose-6-phosphate isomerase Homo sapiens 62-65 8895841-3 1996 In the absence of a Na+ concentration gradient (i.e. when the external Na+ concentration equals the [Nai]), the same concentration (0.5 microM) of nigericin causes the opposite effect on the pHi (acidifies the synaptosomal interior), does not modify the [Nai] and is practically unable to elevate the [Cai] or to increase [3H]GABA release. Nigericin 147-156 glucose-6-phosphate isomerase Homo sapiens 191-194 8967426-8 1996 Exposure to ouabain had no effect on pHi, but it prevented the actions of PTx on PHi in both basal and nigericin-acidified cells. Nigericin 103-112 glucose-6-phosphate isomerase Homo sapiens 81-84 8682873-11 1996 Neutrophils spread normally when the oxidase was blocked or when pHi was clamped near physiological values with nigericin. Nigericin 112-121 glucose-6-phosphate isomerase Homo sapiens 65-68 8967426-10 1996 We conclude that PTx interacts with the Na(+)-K(+)-adenosinetriphosphatase to regulate pHi in both basal and nigericin-acidified Saos-2 cells. Nigericin 109-118 glucose-6-phosphate isomerase Homo sapiens 87-90 1728060-7 1992 Treatment of the cells with nigericin at 0.1-1.0 micrograms/ml lowered the pHi and enhanced the thermosensitivity of the cells in a dose-dependent manner. Nigericin 28-37 glucose-6-phosphate isomerase Homo sapiens 75-78 1476167-3 1992 After acidification with nigericin, thrombin induced an acute increase of [Ca2+]i and a rise in pHi. Nigericin 25-34 glucose-6-phosphate isomerase Homo sapiens 96-99 1728060-9 1992 When a mixture of 1.0 micrograms/ml nigericin, 0.5 mM amiloride, and 0.1 mM DIDS was present in the medium, the pHi rapidly decreased by about 0.3 and 0.4 pH units at pHe 7.2 and 6.6, respectively. Nigericin 36-45 glucose-6-phosphate isomerase Homo sapiens 112-115 8258824-9 1993 Ionophores such as nigericin, CCCP, or propionic acid also lowered the pHi but did not inhibit the oxidative burst, indicating that the pHi drop was not the cause for this inhibition. Nigericin 19-28 glucose-6-phosphate isomerase Homo sapiens 71-74 1332693-5 1992 Nigericin, a K+/H+ ionophore, caused an abrupt fall in pHi to 6.70 +/- 0.07 units. Nigericin 0-9 glucose-6-phosphate isomerase Homo sapiens 55-58 1332693-6 1992 In nigericin-pretreated cells, PMA caused a rapid rise in pHi without changing the [Ca2+]i. Nigericin 3-12 glucose-6-phosphate isomerase Homo sapiens 58-61 3599651-6 1987 Following removal of nigericin, pHi returned to basal levels (pHi = 7.1) when the cells were resuspended in a buffer containing 100 mM Na+. Nigericin 21-30 glucose-6-phosphate isomerase Homo sapiens 32-35 1647205-2 1991 This EIA effect was further synergistically enhanced by lowering intracellular pH (pHi) with either nigericin or sodium propionate, and reversed by raising pHi with monensin or ammonium chloride. Nigericin 100-109 glucose-6-phosphate isomerase Homo sapiens 79-81 1647205-2 1991 This EIA effect was further synergistically enhanced by lowering intracellular pH (pHi) with either nigericin or sodium propionate, and reversed by raising pHi with monensin or ammonium chloride. Nigericin 100-109 glucose-6-phosphate isomerase Homo sapiens 83-86 2171349-3 1990 In HEC that had been acid-loaded using nigericin or a NH4Cl prepulse, pHi recovery occurred via a Na(+)-dependent mechanism that was inhibited by 5-(N-ethyl-N-isopropyl)amiloride (EIPA). Nigericin 39-48 glucose-6-phosphate isomerase Homo sapiens 70-73 24213040-3 1989 A linear relationship was established between pHi and the 490/450 fluorescence ratio of BCECF-AM over the pH range 6.0 to 8.0 using the ionophore nigericin. Nigericin 146-155 glucose-6-phosphate isomerase Homo sapiens 46-49 2548914-5 1989 After acute cytoplasmic acidification by exposure of cells to nigericin in choline+ buffer, or by the abrupt removal of ammonium chloride, complete recovery of pHi occurred in 4-5 min. Nigericin 62-71 glucose-6-phosphate isomerase Homo sapiens 160-163 2831223-6 1988 In the absence of thrombin an increase in pHi, either induced artificially (by addition of the ionophores nigericin or monensin) or via activation of protein kinase C (by addition of 1-oleoyl-2-acetylglycerol), does not induce Ca2+ mobilization. Nigericin 106-115 glucose-6-phosphate isomerase Homo sapiens 42-45 1900792-8 1991 In both single and populational cell types, application of the K(+)-H+ ionophore nigericin, which induced a transient decrease in pHi, led to the release of Ca2+ from intracellular stores. Nigericin 81-90 glucose-6-phosphate isomerase Homo sapiens 130-133 1900792-9 1991 In single parietal cells double-labeled with fura-2 and BCECF, a temporal decrease in pHi preceded the rise in Cai after stimulation with nigericin. Nigericin 138-147 glucose-6-phosphate isomerase Homo sapiens 86-89 3653394-3 1987 Changes of pHi up to +/- 0.35 units, imposed by additions of NH4Cl, CO2 or nigericin, produced no shape change or aggregation and only insignificant changes in [Ca2+]i. Nigericin 75-84 glucose-6-phosphate isomerase Homo sapiens 11-14 3815351-5 1987 Amiloride and 4,4"-diisothiocyanostilbene 2,2-disulfonic acid, inhibitors of the Na+/H+ and HCO3-/Cl- exchangers, respectively, decreased pHi in the presence of nigericin at low pHe. Nigericin 161-170 glucose-6-phosphate isomerase Homo sapiens 138-141 3599651-6 1987 Following removal of nigericin, pHi returned to basal levels (pHi = 7.1) when the cells were resuspended in a buffer containing 100 mM Na+. Nigericin 21-30 glucose-6-phosphate isomerase Homo sapiens 62-65 30613313-4 2018 Changes in the pHi were detected either by microspectrofluorimetry or by a multimode reader with a pH-sensitive fluorescent probe, BCECF, and the fluorescent ratio was calibrated by the high K+/nigericin method. Nigericin 194-203 glucose-6-phosphate isomerase Homo sapiens 15-18 33508621-4 2021 By exchanging H+ and K+ across cell membranes, nigericin shows promising anti-cancer activities in in vitro and in vivo as a single agent or in combination with other anti-cancer drugs through decreasing intracellular pH (pHi). Nigericin 47-56 glucose-6-phosphate isomerase Homo sapiens 222-225 16963074-5 2006 EGFP intensity was found to reflect pHi values of cells at different pHe in the presence of nigericin and was affected by the addition of HCl and NaOH. Nigericin 92-101 glucose-6-phosphate isomerase Homo sapiens 36-39 17200954-8 2007 RESULTS: The pHi clamping conditions were optimized as 140 mM potassium and 10 microM nigericin. Nigericin 86-95 glucose-6-phosphate isomerase Homo sapiens 13-16 29050473-7 2017 Taking advantages of these merits, we employ Eu@SiNRs for the visualization of the cytoplasmic alkalization process mediated by nigericin in living cells, for around 30 min without interruption, revealing important information for understanding the dynamic process of pHi fluctuations. Nigericin 128-137 glucose-6-phosphate isomerase Homo sapiens 268-271 16734821-5 2006 pHi was measured in resting cells, cells acidified with nigericin, and cells treated with thrombin. Nigericin 56-65 glucose-6-phosphate isomerase Homo sapiens 0-3 16734821-8 2006 Nigericin treatment of cells showed that the recovery in pHi was Na+-dependent and followed Michaelis-Menten kinetics. Nigericin 0-9 glucose-6-phosphate isomerase Homo sapiens 57-60