PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 23982515-0 2013 Catalytic mechanism of alpha-phosphate attack in dUTPase is revealed by X-ray crystallographic snapshots of distinct intermediates, 31P-NMR spectroscopy and reaction path modelling. Phosphates 29-38 Deoxyuridine triphosphatase Drosophila melanogaster 49-56 23982515-4 2013 Combining mass spectrometry analysis of the dUTPase-catalyzed reaction carried out in and quantum mechanics/molecular mechanics (QM/MM) simulation, we show that the nucleophilic attack occurs at the alpha-phosphate site. Phosphates 205-214 Deoxyuridine triphosphatase Drosophila melanogaster 44-51 2168898-6 1990 In an effort to establish that phosphate turnover was occurring on the cellular dUTPase protein, cells were prelabeled with 32P-orthophosphate and then infected with HSV in the absence of label. Phosphates 31-40 Deoxyuridine triphosphatase Drosophila melanogaster 80-87 2168898-7 1990 Evidence from this experiment indicates that the phosphate moiety is removed from the cellular dUTPase protein during the infection. Phosphates 49-58 Deoxyuridine triphosphatase Drosophila melanogaster 95-102 21831832-3 2011 Our spectroscopy and transient kinetics results on human dUTPase mutants combined with previous structural studies indicate that (i) H-bond interactions between the gamma-phosphate and the P-loop-like motif V promote the catalytically competent conformation of the reaction center at the alpha-phosphate group; (ii) these interactions accelerate the chemical step of the kinetic cycle and that (iii) hydrolysis occurs very slowly or not at all in the absence of the gamma-phosphate--motif V interactions, i.e., in dUDP, dUDP.BeFx, or in the motif V-deleted mutant. Phosphates 171-180 Deoxyuridine triphosphatase Drosophila melanogaster 57-64 8631817-13 1996 [32P]Orthophosphate-labeled dUTPase was purified from HeLa cells, revealing that only the nuclear form of dUTPase is phosphorylated. Phosphates 5-19 Deoxyuridine triphosphatase Drosophila melanogaster 28-35 8631817-13 1996 [32P]Orthophosphate-labeled dUTPase was purified from HeLa cells, revealing that only the nuclear form of dUTPase is phosphorylated. Phosphates 5-19 Deoxyuridine triphosphatase Drosophila melanogaster 106-113