PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 18952391-6 2008 Insulin was extracted from both the oral and nasal formulations using pH 7.4 phosphate buffer. Phosphates 77-86 insulin Homo sapiens 0-7 19447202-5 2010 The release profile of insulin in phosphate buffer solutions (pH 6.5 and pH 7.2) is completely different than that in acidic medium (pH 1.2). Phosphates 34-43 insulin Homo sapiens 23-30 19101054-5 2009 In addition, serum phosphate levels had a negative correlation with age, body mass index, uric acid, fasting glucose, insulin, HOMA-IR, HS-CRP, triglyceride levels, systolic blood pressure, diastolic blood pressure, and waist circumference (P<0.001). Phosphates 19-28 insulin Homo sapiens 118-125 21528821-0 2011 The relationship between serum phosphate levels with childhood obesity and insulin resistance. Phosphates 31-40 insulin Homo sapiens 75-82 21528821-1 2011 AIM: To investigate the relationship between serum phosphate levels with obesity and insulin resistance in childhood. Phosphates 51-60 insulin Homo sapiens 85-92 21528821-6 2011 CONCLUSIONS: Low serum phosphate levels could contribute to the development of insulin resistance in 6- to 12-year-old obese children. Phosphates 23-32 insulin Homo sapiens 79-86 21694920-7 2009 Insulin and adiponectin are necessary for high-energy phosphate generation. Phosphates 54-63 insulin Homo sapiens 0-7 20093223-6 2009 RESULTS: The study of the release of insulin in phosphate buffer at pH 7.4, showed a continuous release profiles strongly depending on Ethylcellulose/Eudragit report and the initial charge of insulin. Phosphates 48-57 insulin Homo sapiens 37-44 20093223-6 2009 RESULTS: The study of the release of insulin in phosphate buffer at pH 7.4, showed a continuous release profiles strongly depending on Ethylcellulose/Eudragit report and the initial charge of insulin. Phosphates 48-57 insulin Homo sapiens 192-199 18664368-4 2008 Insulin regulates the sub-cellular localization, stability and trans-activation potential of Sp1 by dynamically modulating its post-translational modification by O-linked beta-N-acetylglucosamine (O-GlcNAc) or phosphate residues. Phosphates 210-219 insulin Homo sapiens 0-7 16089501-0 2005 Decreased insulin-stimulated ATP synthesis and phosphate transport in muscle of insulin-resistant offspring of type 2 diabetic parents. Phosphates 47-56 insulin Homo sapiens 10-17 16214071-6 2005 On the other hand, a low-phosphate diet, alkalosis, growth hormone, insulin, IGF-1, and thyroid hormones increase tubular phosphate reabsorption. Phosphates 122-131 insulin Homo sapiens 68-75 18542008-5 2008 Glucose potassium insulin infusions have also produced variable results, and sometimes cause falls in plasma phosphate with potential consequences. Phosphates 109-118 insulin Homo sapiens 18-25 17561792-1 2007 BACKGROUND: Absorption rates of the phosphate-buffered insulin analogs aspart, lispro, and glulisine prevail over that of regular human insulin. Phosphates 36-45 insulin Homo sapiens 55-62 17561792-1 2007 BACKGROUND: Absorption rates of the phosphate-buffered insulin analogs aspart, lispro, and glulisine prevail over that of regular human insulin. Phosphates 36-45 insulin Homo sapiens 136-143 17107927-9 2007 The encapsulated insulin was quickly released in a phosphate buffer saline (pH 7.4), whereas a small amount of insulin was released under acidic condition (0.1N HCl; pH 2.0) because under acidic conditions, carboxylic groups present in the system exist in nonionized form and are poorly hydrophilic. Phosphates 51-60 insulin Homo sapiens 17-24 16391583-0 2006 Association of serum phosphate levels with glucose tolerance, insulin sensitivity and insulin secretion in non-diabetic subjects. Phosphates 21-30 insulin Homo sapiens 62-69 16089501-0 2005 Decreased insulin-stimulated ATP synthesis and phosphate transport in muscle of insulin-resistant offspring of type 2 diabetic parents. Phosphates 47-56 insulin Homo sapiens 80-87 16089501-3 2005 In order to further characterize mitochondrial activity in these individuals, we examined insulin-stimulated rates of adenosine triphosphate (ATP) synthesis and phosphate transport in skeletal muscle in a similar cohort of participants. Phosphates 131-140 insulin Homo sapiens 90-97 16089501-4 2005 METHODS AND FINDINGS: Rates of insulin-stimulated muscle mitochondrial ATP synthase flux and insulin-stimulated increases in concentrations of intramyocellular inorganic phosphate (Pi) were assessed by 31P magnetic resonance spectroscopy (MRS) in healthy, lean, IR offspring of parents with type 2 diabetes and healthy, lean control participants with normal insulin sensitivity. Phosphates 160-179 insulin Homo sapiens 93-100 16089501-4 2005 METHODS AND FINDINGS: Rates of insulin-stimulated muscle mitochondrial ATP synthase flux and insulin-stimulated increases in concentrations of intramyocellular inorganic phosphate (Pi) were assessed by 31P magnetic resonance spectroscopy (MRS) in healthy, lean, IR offspring of parents with type 2 diabetes and healthy, lean control participants with normal insulin sensitivity. Phosphates 160-179 insulin Homo sapiens 93-100 16089501-10 2005 Furthermore, these IR offspring also have impaired insulin-stimulated phosphate transport in muscle, which may contribute to their defects in insulin-stimulated rates of mitochondrial ATP synthesis. Phosphates 70-79 insulin Homo sapiens 51-58 16089501-10 2005 Furthermore, these IR offspring also have impaired insulin-stimulated phosphate transport in muscle, which may contribute to their defects in insulin-stimulated rates of mitochondrial ATP synthesis. Phosphates 70-79 insulin Homo sapiens 142-149 20704943-3 2004 Application of recent advances in high throughput genetics and genetic analysis has revealed evidence for association of protein tyrosine phosphatase N1 gene (PTPN1, a catalytic protein that removes phosphate groups from phosphorylated tyrosine residues) with T2DM and insulin resistance (a condition whereby the body no longer responds to insulin). Phosphates 199-208 insulin Homo sapiens 269-276 20704943-3 2004 Application of recent advances in high throughput genetics and genetic analysis has revealed evidence for association of protein tyrosine phosphatase N1 gene (PTPN1, a catalytic protein that removes phosphate groups from phosphorylated tyrosine residues) with T2DM and insulin resistance (a condition whereby the body no longer responds to insulin). Phosphates 199-208 insulin Homo sapiens 340-347 15375778-15 2004 Serum levels of Cai increased and serum phosphate levels decreased in response to insulin-induced hypoglycemia, while serum phosphate levels were also independently influenced by time decreasing between 9 AM and 12 PM (P < .05). Phosphates 40-49 insulin Homo sapiens 82-89 12653342-9 2003 Treatment of calcium and phosphate disturbances, including vitamin D therapy, significantly reduces insulin resistance in uremia. Phosphates 25-34 insulin Homo sapiens 100-107 15523542-0 2004 Benefits of insulin aspart vs phosphate-buffered human regular insulin in persons with type 1 Diabetes treated by means of an insulin pump. Phosphates 30-39 insulin Homo sapiens 63-70 15523542-0 2004 Benefits of insulin aspart vs phosphate-buffered human regular insulin in persons with type 1 Diabetes treated by means of an insulin pump. Phosphates 30-39 insulin Homo sapiens 63-70 15523542-1 2004 UNLABELLED: Absorption rates of phosphate buffered insulin analogs aspart and lispro prevail over regular human insulin. Phosphates 32-41 insulin Homo sapiens 51-58 15078585-7 2004 Insulin resistance in heart failure can be detrimental, because transcriptional shifts in metabolic gene expression favor glucose over fat as a substrate for high-energy phosphate production. Phosphates 170-179 insulin Homo sapiens 0-7 11430833-2 2001 Here, we demonstrate that the insulin-induced inhibition of GSK3 and its unique substrate specificity are explained by the existence of a phosphate binding site in which Arg-96 is critical. Phosphates 138-147 insulin Homo sapiens 30-37 12916897-6 2003 The relative pulmonary bioactivity of insulin in phosphate buffer pH 7.4 and citrate buffer pH 3.5 was 11.36% +/- 1.27% and 43.20% +/- 2.48%, respectively, compared to subcutaneous administration. Phosphates 49-58 insulin Homo sapiens 38-45 10408765-9 1999 These findings suggest that hyperinsulinemia and insulin-induced transmembrane shift of extracellular potassium and phosphate may have been involved in the abnormalities of serum electrolytes and development of hypokalemic periodic paralysis in the present patient. Phosphates 116-125 insulin Homo sapiens 33-40 11292543-5 2001 Dissolution studies conducted using different ionic species (acetate, phosphate, citrate and EDTA) in a spin-filter device demonstrated a rank order correlation for different sources of zinc insulin; it was observed that human zinc insulin dissolved faster than bovine insulin, these differences attributed to their binding properties and the respective affinities to the various ionic species used. Phosphates 70-79 insulin Homo sapiens 232-239 10905295-7 2000 The IrOx film electrode was used as an amperometric detector for flow injection analysis of insulin in pH 7.40 phosphate buffer. Phosphates 111-120 insulin Homo sapiens 92-99 10210724-12 1999 The conformational change of insulin was effectively prevented in PEG-g-HA solutions, although insulin was denatured in storage of both phosphate buffered solution and HA solution. Phosphates 136-145 insulin Homo sapiens 29-36 10210724-12 1999 The conformational change of insulin was effectively prevented in PEG-g-HA solutions, although insulin was denatured in storage of both phosphate buffered solution and HA solution. Phosphates 136-145 insulin Homo sapiens 95-102 10726078-2 1999 Randall"s plaques have returned, phosphate relates to insulin and lipid metabolism, and sialic acid is out. Phosphates 33-42 insulin Homo sapiens 54-61 9794561-2 1998 Insulin plays a major role in the maintenance of phosphate homeostasis but it remains to be determined whether in uraemia insulin-dependent renal and extrarenal phosphate disposal is also affected. Phosphates 49-58 insulin Homo sapiens 0-7 9794561-7 1998 RESULTS: The tissue insulin sensitivity (M/I) was lower in patients with renal failure than in control subjects (5.3+/-2.4 vs 6.7+/-1.8mg/kg/min per mU/ml, P= 0.001) but the phosphate lowering action of insulin was larger in patients with renal failure than in control subjects. Phosphates 174-183 insulin Homo sapiens 20-27 9794561-10 1998 A significant correlation was observed between changes in serum phosphate and PTH induced by hyperinsulinaemia (r = 0.48, P < 0.01 ) CONCLUSIONS: Phosphate-lowering effect of insulin is well preserved in severe renal failure despite the resistance to insulin-stimulated glucose uptake. Phosphates 64-73 insulin Homo sapiens 98-105 9660090-1 1998 Phosphate is an active participant in energy metabolism, and its deficiency has been associated with changes in insulin sensitivity and glucose tolerance. Phosphates 0-9 insulin Homo sapiens 112-119 9794561-10 1998 A significant correlation was observed between changes in serum phosphate and PTH induced by hyperinsulinaemia (r = 0.48, P < 0.01 ) CONCLUSIONS: Phosphate-lowering effect of insulin is well preserved in severe renal failure despite the resistance to insulin-stimulated glucose uptake. Phosphates 64-73 insulin Homo sapiens 178-185 9794561-10 1998 A significant correlation was observed between changes in serum phosphate and PTH induced by hyperinsulinaemia (r = 0.48, P < 0.01 ) CONCLUSIONS: Phosphate-lowering effect of insulin is well preserved in severe renal failure despite the resistance to insulin-stimulated glucose uptake. Phosphates 149-158 insulin Homo sapiens 98-105 9794561-10 1998 A significant correlation was observed between changes in serum phosphate and PTH induced by hyperinsulinaemia (r = 0.48, P < 0.01 ) CONCLUSIONS: Phosphate-lowering effect of insulin is well preserved in severe renal failure despite the resistance to insulin-stimulated glucose uptake. Phosphates 149-158 insulin Homo sapiens 178-185 9660090-6 1998 The presence of hyperinsulinemia both basally and after glucose stimulation, with normal glycemia, in phosphate-depleted individuals suggests that this condition is associated with reduced insulin sensitivity. Phosphates 102-111 insulin Homo sapiens 21-28 1649719-15 1991 The results also suggest that insulin causes a primary increase in the next flux of inorganic phosphate across the muscle cell membrane. Phosphates 84-103 insulin Homo sapiens 30-37 8858098-7 1996 We find that following insulin stimulation (i) 90% of the receptor-associated phosphate is located in the endosomal compartment, (ii) the endosomal receptor is most highly phosphorylated in the tyrosine kinase domain, and (iii) significant levels of juxtamembrane domain phosphorylation are detected in the endosomal receptor. Phosphates 78-87 insulin Homo sapiens 23-30 8637455-0 1996 Effect of insulin on urinary phosphate excretion in type II diabetes mellitus with or without renal insufficiency. Phosphates 29-38 insulin Homo sapiens 10-17 8637455-1 1996 We investigated the effect of insulin on urinary excretion of phosphate in type II diabetes mellitus (DM) with respect to the absence or presence of renal insufficiency. Phosphates 62-71 insulin Homo sapiens 30-37 8550745-0 1996 Changes in plasma phosphate levels influence insulin sensitivity under euglycemic conditions. Phosphates 18-27 insulin Homo sapiens 45-52 8889763-5 1996 We observed that insulin infusion significantly increased the rate of systemic glucose utilization (P < 0.01) and also significantly decreased the ratio of inorganic phosphate to phosphocreatine (P < 0.001) during forearm exercise compared with the control study. Phosphates 159-178 insulin Homo sapiens 17-24 8928187-1 1995 The effect of acute insulin infusion on the metabolism of calcium (Ca) and phosphate (P) was examined in 17 healthy subjects. Phosphates 75-84 insulin Homo sapiens 20-27 8928187-1 1995 The effect of acute insulin infusion on the metabolism of calcium (Ca) and phosphate (P) was examined in 17 healthy subjects. Phosphates 86-87 insulin Homo sapiens 20-27 7635975-3 1995 Approximately 50% of PCOS women (PCOS-Ser) had a significant increase in insulin-independent beta-subunit [32P]phosphate incorporation (3.7-fold, P < 0.05 vs other groups) in skin fibroblast insulin receptors that was present in serine residues while insulin-induced tyrosine phosphorylation was decreased (both P < 0.05 vs other groups). Phosphates 111-120 insulin Homo sapiens 73-80 7635975-3 1995 Approximately 50% of PCOS women (PCOS-Ser) had a significant increase in insulin-independent beta-subunit [32P]phosphate incorporation (3.7-fold, P < 0.05 vs other groups) in skin fibroblast insulin receptors that was present in serine residues while insulin-induced tyrosine phosphorylation was decreased (both P < 0.05 vs other groups). Phosphates 111-120 insulin Homo sapiens 194-201 7635975-3 1995 Approximately 50% of PCOS women (PCOS-Ser) had a significant increase in insulin-independent beta-subunit [32P]phosphate incorporation (3.7-fold, P < 0.05 vs other groups) in skin fibroblast insulin receptors that was present in serine residues while insulin-induced tyrosine phosphorylation was decreased (both P < 0.05 vs other groups). Phosphates 111-120 insulin Homo sapiens 194-201 7929343-10 1994 Kinetic analysis revealed that the addition of phosphate to the serine improved substrate recognition by the insulin receptor tyrosine kinase almost 2-fold. Phosphates 47-56 insulin Homo sapiens 109-116 8195122-8 1994 Both beta and beta delta-subunits of the Leu323-delta 43 hybrid receptor are phosphorylated in vivo and in vitro in an insulin-dependent manner, suggesting an intramolecular transphosphorylation mechanism and that the presence of the Leu323 mutant receptor that lacks an intrinsic high affinity binding site does not prevent the associated beta-subunit from functioning either as a tyrosine kinase or as a phosphate acceptor in the hybrid insulin receptor molecule (alpha alpha mut beta delta beta). Phosphates 406-415 insulin Homo sapiens 119-126 1649719-0 1991 Effect of insulin on intracellular pH and phosphate metabolism in human skeletal muscle in vivo. Phosphates 42-51 insulin Homo sapiens 10-17 2200676-5 1990 After autophosphorylation of hormone-bound (alpha beta)2-insulin receptors, phosphate incorporation was found for 7.9-nm receptor forms when receptor-insulin complexes were crosslinked with disuccinimide suberate prior to Triton X-100/PAGE. Phosphates 76-85 insulin Homo sapiens 57-64 1870424-5 1991 At any given insulin level, the obese individuals excreted significantly more calcium, phosphate, and potassium per minute than lean controls. Phosphates 87-96 insulin Homo sapiens 13-20 2049186-0 1991 Extracellular phosphate requirement for insulin action on isolated rat hepatocytes. Phosphates 14-23 insulin Homo sapiens 40-47 2049186-4 1991 The maximal insulin stimulatory effect on succinate oxidation and tracer incorporation into protein was observed in the presence of 1.18 mM phosphate and 1.9 mM calcium ion. Phosphates 140-149 insulin Homo sapiens 12-19 2245873-6 1990 Because glyceraldehyde, which enters metabolism at the triose phosphates in the glycolytic pathway, is a potent insulin secretagogue but pyruvate, which is metabolized in the mitochondrion, is not an insulin secretagogue, the proximal signal for glucose-induced insulin release originates with an interaction between the central part of the glycolytic pathway and mitochondrial metabolism. Phosphates 62-72 insulin Homo sapiens 112-119 2200676-5 1990 After autophosphorylation of hormone-bound (alpha beta)2-insulin receptors, phosphate incorporation was found for 7.9-nm receptor forms when receptor-insulin complexes were crosslinked with disuccinimide suberate prior to Triton X-100/PAGE. Phosphates 76-85 insulin Homo sapiens 150-157 2200676-6 1990 However, phosphate incorporation was demonstrated for the 9.5-nm receptor forms when receptor-insulin complexes were not prevented from dissociation. Phosphates 9-18 insulin Homo sapiens 94-101 2515069-5 1989 Insulin stimulated [32P]phosphate incorporation into the 45 kDa protein approximately 2-fold. Phosphates 24-33 insulin Homo sapiens 0-7 34884501-0 2021 A Novel PTP1B Inhibitor-Phosphate of Polymannuronic Acid Ameliorates Insulin Resistance by Regulating IRS-1/Akt Signaling. Phosphates 24-33 insulin Homo sapiens 69-76 2504716-8 1989 Surprisingly, the insulin-independent activated (1 min in vivo insulin-treated) and uncoupled (24 h in vivo insulin-treated) insulin receptors displayed similar stoichiometries of 32P incorporation into the beta subunit by in vitro autophosphorylation when compared with the control insulin receptors, ranging from 1.5 to 1.8 mol of phosphate incorporated/mol of insulin receptor. Phosphates 333-342 insulin Homo sapiens 18-25 2546561-4 1989 In contrast, the dimer only partially (23%) mimicked insulin"s effect on phosphate incorporation into insulin receptors immunoprecipitated after equilibration of cells with [32P]phosphate. Phosphates 73-82 insulin Homo sapiens 53-60 2667802-4 1989 The mean decrease in the concentration of phosphate in plasma, measured as an index of glucose uptake by cells, was significantly less (P less than 0.05) 2 h after the load in the group with flatter glucose responses, suggesting that the flat response is ascribable to poor glucose absorption rather than to an exaggerated insulin response. Phosphates 42-51 insulin Homo sapiens 323-330 2546561-4 1989 In contrast, the dimer only partially (23%) mimicked insulin"s effect on phosphate incorporation into insulin receptors immunoprecipitated after equilibration of cells with [32P]phosphate. Phosphates 73-82 insulin Homo sapiens 102-109 2546561-4 1989 In contrast, the dimer only partially (23%) mimicked insulin"s effect on phosphate incorporation into insulin receptors immunoprecipitated after equilibration of cells with [32P]phosphate. Phosphates 178-187 insulin Homo sapiens 53-60 2546561-4 1989 In contrast, the dimer only partially (23%) mimicked insulin"s effect on phosphate incorporation into insulin receptors immunoprecipitated after equilibration of cells with [32P]phosphate. Phosphates 178-187 insulin Homo sapiens 102-109 3536929-7 1986 Exposure of cells to both insulin and PMA resulted in a 3-fold increase in the level of phosphate in GT compared to that seen with PMA alone. Phosphates 88-97 insulin Homo sapiens 26-33 2659084-4 1989 Insulin was also found to increase the incorporation of phosphate into phospholipids, but only if its concentration was at least 100-times higher than that of IGF-I. Phosphates 56-65 insulin Homo sapiens 0-7 2660785-3 1989 Addition of insulin increased phosphate incorporation into calmodulin 2.5 fold. Phosphates 30-39 insulin Homo sapiens 12-19 2546843-0 1989 Influence of insulin on phosphate uptake by brush border membranes from human placenta. Phosphates 24-33 insulin Homo sapiens 13-20 2546843-1 1989 Regulation of phosphate transport by insulin was investigated in brush border membranes from human placenta at term. Phosphates 14-23 insulin Homo sapiens 37-44 2546843-2 1989 At 22 degrees C, a 45 min incubation of the total tissue with 10(-6) M insulin significantly decreased both the initial rate and the peak of sodium-dependent phosphate uptake by the corresponding brush border membranes. Phosphates 158-167 insulin Homo sapiens 71-78 2546843-4 1989 Increasing the insulin concentration from 0 to 10(-5) M resulted in a dose-dependent inhibition of phosphate uptake with half-maximal effect at 1.1 x 10(-9) M. The hormone decreased PO4 transport by decreasing the affinity of the carrier for the substrate (Km = 0.180 +/- 0.010 mM and 0.215 +/- 0.015 mM in absence and presence of 10(-6) M insulin respectively, P less than 0.05). Phosphates 99-108 insulin Homo sapiens 15-22 2546843-4 1989 Increasing the insulin concentration from 0 to 10(-5) M resulted in a dose-dependent inhibition of phosphate uptake with half-maximal effect at 1.1 x 10(-9) M. The hormone decreased PO4 transport by decreasing the affinity of the carrier for the substrate (Km = 0.180 +/- 0.010 mM and 0.215 +/- 0.015 mM in absence and presence of 10(-6) M insulin respectively, P less than 0.05). Phosphates 99-108 insulin Homo sapiens 340-347 2900139-8 1988 The total phosphate content of acetyl-CoA carboxylase, isolated from adipocytes in the presence of protein phosphatase inhibitors, was estimated by 32P-labelling experiments to be 2.6 +/- 0.1 (5), 3.4 +/- 0.2 (5), and 3.8 +/- 0.2 (3) mol/mol subunit for enzyme from control, insulin- and TPA-treated cells respectively. Phosphates 10-19 insulin Homo sapiens 275-282 2900140-4 1988 One of these (T4a) accounts for a large part of the total increase in phosphate observed after insulin treatment, and comigrates with the peptide containing the sites phosphorylated in vitro by casein kinase-2. Phosphates 70-79 insulin Homo sapiens 95-102 2900140-5 1988 The other may correspond to the "I" site peptide originally described by Brownsey and Denton in 1982: labelling of this peptide is stimulated at least threefold by insulin treatment, but it is a minor phosphopeptide and, even after insulin treatment, accounts for only about 2.5% of the enzyme-bound phosphate (equivalent to less than 0.1 mol phosphate/mol 240-kDa subunit). Phosphates 300-309 insulin Homo sapiens 164-171 2900140-5 1988 The other may correspond to the "I" site peptide originally described by Brownsey and Denton in 1982: labelling of this peptide is stimulated at least threefold by insulin treatment, but it is a minor phosphopeptide and, even after insulin treatment, accounts for only about 2.5% of the enzyme-bound phosphate (equivalent to less than 0.1 mol phosphate/mol 240-kDa subunit). Phosphates 343-352 insulin Homo sapiens 164-171 3546299-6 1987 Prephosphorylation of the insulin receptor (from 0 to 1.3 mol of phosphate/mol of insulin receptor) in the absence of insulin was found to have no significant effect on the exogenous protein kinase activity when assayed both in the presence and absence of insulin. Phosphates 65-74 insulin Homo sapiens 26-33 3546299-8 1987 In contrast, prephosphorylation of the insulin receptors in the presence of insulin was observed to enhance the exogenous protein kinase activity dependent on the extent of autophosphorylation, such that by 1.4 mol of phosphate incorporated per mol of insulin receptor, insulin was found to maximally stimulate the initial rate of Glu:Tyr phosphorylation (approximately 9-fold). Phosphates 218-227 insulin Homo sapiens 39-46 3546299-8 1987 In contrast, prephosphorylation of the insulin receptors in the presence of insulin was observed to enhance the exogenous protein kinase activity dependent on the extent of autophosphorylation, such that by 1.4 mol of phosphate incorporated per mol of insulin receptor, insulin was found to maximally stimulate the initial rate of Glu:Tyr phosphorylation (approximately 9-fold). Phosphates 218-227 insulin Homo sapiens 76-83 3546299-8 1987 In contrast, prephosphorylation of the insulin receptors in the presence of insulin was observed to enhance the exogenous protein kinase activity dependent on the extent of autophosphorylation, such that by 1.4 mol of phosphate incorporated per mol of insulin receptor, insulin was found to maximally stimulate the initial rate of Glu:Tyr phosphorylation (approximately 9-fold). Phosphates 218-227 insulin Homo sapiens 76-83 2675887-4 1989 The decisive advantage of cardiac nutrition using glucose and insulin lies in an anabolic action with resynthesis of high energetic phosphates for improving the inotropic capacity of the heart. Phosphates 132-142 insulin Homo sapiens 62-69 3118700-5 1987 This complication was likely caused by tissue anabolism as well as by CHO-stimulated, insulin-induced intracellular shifts of phosphate. Phosphates 126-135 insulin Homo sapiens 86-93 3804434-6 1986 We suppose that in the intact organism the pharmacologic effect of dopamine on the tubular handling of phosphate which seems to be experimentally proven, may be masked by other mechanisms with an antiphosphaturic action such as increased levels of growth hormone and/or insulin. Phosphates 103-112 insulin Homo sapiens 270-277 2881538-3 1986 Experiments with 32P-labelled adipocytes showed that insulin increased the total phosphorylation of acetyl-CoA carboxylase from 2.7 to 3.5 molecules of phosphate/240 kDa subunit, and confirmed that this increase was partially accounted for by phosphorylation within a specific peptide (the "I-site" peptide). Phosphates 152-161 insulin Homo sapiens 53-60 2988200-1 1985 In the particles enriched with plasmatic membranes of target cells (human erythrocytes, skeletal muscles and adipocytes of rats) ATP was steadily formed within 1 min of incubation of the particles with insulin (4 microgram/ml) in the medium containing Tris-HCl buffer, pH 7.5, ADP, Mg2+, inorganic phosphate, NaF, during NADH oxidation in presence of cytochrome c and oxygen (30 degrees). Phosphates 288-307 insulin Homo sapiens 202-209 2997784-7 1985 The insulin-mediated decrease in the [32P]phosphate content of IGF-II receptors from the plasma membrane was rapid in onset, paralleled the increase in the number of IGF-II receptors on the cell surface, and persisted for at least 30 min in the presence of insulin. Phosphates 42-51 insulin Homo sapiens 4-11 3920212-4 1985 MAP2 was found to be the best substrate among the cytoskeletal proteins tested; in the presence of insulin, the Vmax for MAP2 was 6.3 nmol/min/mg, its Km was 5.1 microM, and 1.7 mol of phosphate were incorporated per mol of MAP2. Phosphates 185-194 insulin Homo sapiens 99-106 3933482-8 1985 Phosphate uptake was stimulated when cells were cultured in the presence of insulin, and was also affected by changes in phosphate concentrations in cultured medium. Phosphates 0-9 insulin Homo sapiens 76-83 6097186-8 1984 This human putative second messenger of insulin action was eluted from the anion-exchange resin AG1-X8 at an ionic strength of 0.3-0.4 M, as well as from the hydroxylapatite column at a phosphate concentration of 0.2-0.3 M. Phosphates 186-195 insulin Homo sapiens 40-47 6384712-1 1984 Insulin action on [32P]-phosphate incorporation into brain membranes was determined. Phosphates 24-33 insulin Homo sapiens 0-7 6090205-1 1984 Insulin receptor preparations from human placenta at various states of purity were shown to catalyze insulin-stimulated phosphate incorporation from [gamma-32P]ATP into endogenous (membrane) and exogenous phosphatidylinositol. Phosphates 120-129 insulin Homo sapiens 101-108 6146684-1 1984 A method is described which measures the prolongation effects of commercial insulin suspensions by monitoring the rate of solution of an insulin suspension into a phosphate buffer, pH 7.4, at 37 degrees C. The method can rapidly categorize an insulin into the clinical classifications of either fast, intermediate or slow acting. Phosphates 163-172 insulin Homo sapiens 76-83 6146684-1 1984 A method is described which measures the prolongation effects of commercial insulin suspensions by monitoring the rate of solution of an insulin suspension into a phosphate buffer, pH 7.4, at 37 degrees C. The method can rapidly categorize an insulin into the clinical classifications of either fast, intermediate or slow acting. Phosphates 163-172 insulin Homo sapiens 137-144 6146684-1 1984 A method is described which measures the prolongation effects of commercial insulin suspensions by monitoring the rate of solution of an insulin suspension into a phosphate buffer, pH 7.4, at 37 degrees C. The method can rapidly categorize an insulin into the clinical classifications of either fast, intermediate or slow acting. Phosphates 163-172 insulin Homo sapiens 137-144 6203118-3 1984 Its Vmax for phosphorylating angiotensin is 80 nmol of phosphate per min per mg of protein at 23 degrees C. The procedure used to purify the receptor includes chromatography on wheat germ agglutinin-agarose and on insulin-Sepharose. Phosphates 55-64 insulin Homo sapiens 214-221 6203118-9 1984 In the presence of insulin, approximately 2 mol of phosphate was incorporated per mol of beta subunit. Phosphates 51-60 insulin Homo sapiens 19-26 6389240-5 1984 Insulin delivered to the latter sites stimulates several important processes, including reabsorption of sodium, phosphate, and glucose. Phosphates 112-121 insulin Homo sapiens 0-7 6761184-0 1982 Inorganic phosphate-insulin relationships in normal subjects and in patients with moderate glucose intolerance. Phosphates 0-19 insulin Homo sapiens 20-27 6582851-9 1983 IGF-I shows cross-reactivity with the insulin receptor, with a potency of 12 and 100 times less than insulin in Krebs-Ringer phosphate buffer and G-buffer respectively. Phosphates 125-134 insulin Homo sapiens 38-45 6341773-6 1983 After insulin was infused for four hours, serum phosphate had decreased in all subjects (P less than 0.001) and strongly correlated with glucose disposal rates (r = 0.76, P less than 0.005). Phosphates 48-57 insulin Homo sapiens 6-13 6341773-9 1983 The biologic effect of insulin on glucose utilization and plasma phosphate shifts is clearly diminished. Phosphates 65-74 insulin Homo sapiens 23-30 6358209-11 1983 When insulin binding was performed under the same conditions as the phosphorylation, half-maximal stimulation of phosphate incorporation occurred with 6-29% of the fractional occupancy of the receptor. Phosphates 113-122 insulin Homo sapiens 5-12 6761184-1 1982 The relationship between serum insulin and serum inorganic phosphate levels was investigated in normal subjects (NGT) and in patients with moderate impairment of glucose tolerance (IGT1 with subnormal and IGT2 with excessive insulin secretion) fasting and after oral glucose. Phosphates 49-68 insulin Homo sapiens 31-38 6761184-3 1982 In NGT subjects, a significant correlation between fasting insulin and inorganic phosphate levels are observed. Phosphates 71-90 insulin Homo sapiens 59-66 6761184-4 1982 In NGT and in IGT1 subjects, oral glucose administration was followed by a fall in serum phosphate level that was well correlated with the total insulin released. Phosphates 89-98 insulin Homo sapiens 145-152 1176581-9 1975 A significant and sustained decline in the serum inorganic phosphate levels occurred following insulin administration. Phosphates 49-68 insulin Homo sapiens 95-102 7024029-0 1981 The effect of chronic insulin therapy on phosphate metabolism in diabetes mellitus. Phosphates 41-50 insulin Homo sapiens 22-29 1230136-3 1975 The insulin-like effect of lithium on carbohydrate metabolism and correlated ions (phosphates, calcium, magnesium, potassium) at cell membrane level is then discussed. Phosphates 83-93 insulin Homo sapiens 4-11 6759176-3 1982 Insulin stimulates phosphate incorporation into the Mr 95 000 subunit probably by activation of the phosphorylation step. Phosphates 19-28 insulin Homo sapiens 0-7 6802694-8 1981 Phosphate buffered highly purified porcine insulin is suitable for relatively prolonged infusion if metal ion contamination of the delivery system is minimised. Phosphates 0-9 insulin Homo sapiens 43-50 105228-0 1979 Effects of insulin infusion on plasma phosphate in diabetic patients. Phosphates 38-47 insulin Homo sapiens 11-18 105228-3 1979 Therefore this study compared the effects of different rates of insulin infusion on the changes in plasma phosphate concentration in ketotic, hyperglycemic diabetic man. Phosphates 106-115 insulin Homo sapiens 64-71 105228-7 1979 We observed that plasma phosphate concentrations declined significantly only with low-dose and high-dose insulin infusions. Phosphates 24-33 insulin Homo sapiens 105-112 105228-8 1979 The magnitude and rapidity of fall of the mean phosphate concentration were greatest with high-dose insulin infusion. Phosphates 47-56 insulin Homo sapiens 100-107 356269-1 1978 Perifusion experiments have shown that there is a discharge of inorganic phosphate into the medium when insulin secretion from isolated islets is stimulated by glucose. Phosphates 63-82 insulin Homo sapiens 104-111 645445-6 1978 A significant, positive correlation of serum inorganic phosphate with the early insulin response in the i.v. Phosphates 45-64 insulin Homo sapiens 80-87 1201102-0 1975 Metal ion- and phosphate-mediated transport of glucose by insulin. Phosphates 15-24 insulin Homo sapiens 58-65 4973285-0 1967 [Stimulation of the formation of macroergic phosphates in skeletal muscle and liver of animals after administration of insulin and vitamin E]. Phosphates 44-54 insulin Homo sapiens 119-126 1130556-4 1975 Further studies were performed in which insulin was also shown to have a rapid stimulatory effect on the incorporation of [32-P] phosphate into RNA; however, the uptake of the labeled phosphate was not affected by insulin. Phosphates 129-138 insulin Homo sapiens 40-47 1229805-4 1975 These findings suggest that changes in plasma pyruvate, lactate and inorganic phosphates induced by insulin, and regarded as espressions of its peripheral metabolism, are greatly dependent on the beta-adrenergic effect of the endogenous catecholamines released during the time when blood glucose values are low. Phosphates 78-88 insulin Homo sapiens 100-107 5792362-5 1969 Evidence is presented that a relatively intact insulin molecule competes with Ca(++) for the free phosphate group of the monolayer. Phosphates 98-107 insulin Homo sapiens 47-54 5880356-0 1965 The mechanism of insulin action: the effect of insulin on phosphate turnover in red-cell systems. Phosphates 58-67 insulin Homo sapiens 17-24 5880356-0 1965 The mechanism of insulin action: the effect of insulin on phosphate turnover in red-cell systems. Phosphates 58-67 insulin Homo sapiens 47-54 1120786-8 1975 Urinary potassium (UKV) and phosphate (UPV) excretion were also both decreased during insulin administration; UKV decreased from 66 plus or minus 9 to 21 plus or minus 1 mueq/min (P smaller than 0.005), and tupv decreased from 504 plus or minus 93 to 230 plus or minus 43 mug/min (P smaller than 0.01). Phosphates 28-37 insulin Homo sapiens 86-93 13713139-0 1961 [Studies on the effect of insulin on renal phosphate excretion]. Phosphates 43-52 insulin Homo sapiens 26-33 13175204-0 1954 The effect of insulin on the excretion of glucose and phosphate by the kidney of the cat. Phosphates 54-63 insulin Homo sapiens 14-21 14020469-0 1963 The action of insulin on the blood potassium and phosphate in endocrine disorders. Phosphates 49-58 insulin Homo sapiens 14-21 13224662-0 1954 Response of serum inorganic phosphate to insulin in normal and diabetic subjects. Phosphates 18-37 insulin Homo sapiens 41-48 14921810-0 1952 Effect of insulin in vitro on phosphate uptake by erythrocytes from diabetic humans. Phosphates 30-39 insulin Homo sapiens 10-17 21434171-0 1944 The Relation of Insulin to Phosphate Metabolism. Phosphates 27-36 insulin Homo sapiens 16-23 13114413-0 1953 Insulin and the relation between phosphate transport and glucose metabolism. Phosphates 33-42 insulin Homo sapiens 0-7 13421668-0 1957 Enhancement of oxidative esterification of inorganic phosphate by clinical insulin. Phosphates 43-62 insulin Homo sapiens 75-82 20993936-0 1946 The action of insulin on the metabolism of phosphates. Phosphates 43-53 insulin Homo sapiens 14-21 16746242-0 1936 The action of phenyl isocyanate on insulin: Further observations on the chemistry of insulin and its phosphate-lowering power. Phosphates 101-110 insulin Homo sapiens 35-42 16746242-0 1936 The action of phenyl isocyanate on insulin: Further observations on the chemistry of insulin and its phosphate-lowering power. Phosphates 101-110 insulin Homo sapiens 85-92 16743349-0 1924 The Relationship of Phosphates to Carbohydrate Metabolism: Time Relationship of the Changes in Phosphate Excretion caused by Insulin and Sugar. Phosphates 20-30 insulin Homo sapiens 125-132 16743349-0 1924 The Relationship of Phosphates to Carbohydrate Metabolism: Time Relationship of the Changes in Phosphate Excretion caused by Insulin and Sugar. Phosphates 20-29 insulin Homo sapiens 125-132 32654423-0 2021 Impaired cardiac and neurological function with mild hypophosphatemia during insulin therapy for diabetic ketoacidosis and marked improvement with phosphate supplementation: A case report. Phosphates 57-66 insulin Homo sapiens 77-84 16993588-0 1923 On the effect of insulin on blood phosphate. Phosphates 34-43 insulin Homo sapiens 17-24 15391085-0 1949 Nature of the action of insulin on the level of serum inorganic phosphate. Phosphates 54-73 insulin Homo sapiens 24-31 32654423-4 2021 Since overlapping conditions that result in hypophosphatemia can cause severe complications after insulin therapy for DKA, even in patients with mild hypophosphatemia, physicians should pay more attention to changes in phosphate levels in patients undergoing treatment for DKA. Phosphates 48-57 insulin Homo sapiens 98-105 31686640-2 2020 Phosphorus, an essential micronutrient, is closely linked to the cellular metabolism of glucose for energy production, and serum inorganic phosphate is often transported into cells along with glucose during insulin therapy. Phosphates 139-148 insulin Homo sapiens 207-214 33133389-3 2020 AIM: To determine the association between serum P and serum glucose or insulin resistance in diabetic and non-diabetic patients. Phosphates 48-49 insulin Homo sapiens 71-78 32903642-2 2020 Patients with T2D usually have low serum phosphate level due to diet control, osmotic diuresis, and insulin stimulation. Phosphates 41-50 insulin Homo sapiens 100-107 33533767-6 2021 The fabricated electrode was applied for the trace analysis of insulin using the ultrasensitive ECL method in a phosphate buffer solution. Phosphates 112-121 insulin Homo sapiens 63-70 32572402-2 2020 A novel platform of oral insulin delivery is herein proposed based on amphiphilic cholesterol-phosphate conjugate (CHP). Phosphates 94-103 insulin Homo sapiens 25-32 31776845-2 2020 Hypophosphatemia, defined as serum phosphate levels below 2.5 mg/dL (0.81 mmol/L), is frequently observed in the course of treatment with commonly used drugs, such as diuretics, bisphosphonates, antibiotics, insulin, and antacids. Phosphates 4-13 insulin Homo sapiens 208-215 32261517-7 2014 BCC and insulin formed supramolecular aggregates with significantly different morphologies depending on the buffer species used: a network structure in acetate buffer, nanoparticles in citrate buffer, and large aggregates in phosphate buffer. Phosphates 225-234 insulin Homo sapiens 8-15 25827151-4 2015 The pathophysiological mechanism comprises an increase in insulin levels, resulting in shifts of phosphate, potassium and magnesium into the intracellular environment, as well as fluid retention and relative deficiency of vitamin B1. Phosphates 97-106 insulin Homo sapiens 58-65 24763850-1 2014 AIMS/HYPOTHESIS: Low phosphate and high calcium concentrations have been linked to altered glucose tolerance and reduced insulin sensitivity in non-diabetic individuals. Phosphates 21-30 insulin Homo sapiens 121-128 29140513-2 2018 We hypothesized that intrahepatocellular gamma-adenosine triphosphate (gammaATP) and inorganic phosphate (Pi) concentrations exhibit similar associations with insulin sensitivity in nondiabetic, nonobese volunteers. Phosphates 85-104 insulin Homo sapiens 159-166 25782584-7 2015 After adjustment for body mass index, cholesterol, diabetes, adiponectin, calcium, and phosphate, C-peptide was still significantly associated with CAC in a multivariate logistic regression model. Phosphates 87-96 insulin Homo sapiens 98-107 25611002-3 2015 Insulin resistance has been reported and linked to low serum phosphate levels in animal models and studies in diabetic outpatients, but not in postoperative patients. Phosphates 61-70 insulin Homo sapiens 0-7 22826313-2 2012 Measurement of flux between inorganic phosphate (Pi) and ATP using (31)P MRS magnetization transfer has been used in resting muscle to assess what is claimed to be mitochondrial ATP synthesis and has been particularly popular in the study of insulin effects and insulin resistance. Phosphates 28-47 insulin Homo sapiens 242-249 23093656-4 2012 Observations from our group and others suggest that the inorganic phosphate (P(i)) ATP flux in skeletal muscle may be modulated by certain conditions, including aging, insulin resistance, and diabetes, and may reflect inherent alterations in mitochondrial metabolism. Phosphates 66-75 insulin Homo sapiens 170-177 22826313-2 2012 Measurement of flux between inorganic phosphate (Pi) and ATP using (31)P MRS magnetization transfer has been used in resting muscle to assess what is claimed to be mitochondrial ATP synthesis and has been particularly popular in the study of insulin effects and insulin resistance. Phosphates 28-47 insulin Homo sapiens 262-269 22373818-3 2012 The model protein insulin can be rapidly and efficiently encapsulated by the synthesized polymer in aqueous phosphate buffer at physiological pH. Phosphates 108-117 insulin Homo sapiens 18-25 22133206-1 2012 BACKGROUND: Phosphate homeostasis is connected to glucose metabolism and is influenced by insulin, but the role of fibroblast growth factor 23 (FGF23) is unknown in this relation. Phosphates 12-21 insulin Homo sapiens 90-97 22133206-9 2012 In the overall group insulin infusion suppressed plasma phosphate concentrations (p = 0.006), but with no differences between the groups. Phosphates 56-65 insulin Homo sapiens 21-28 22133206-11 2012 The interplay between insulin effects and FGF23 may be important for the understanding of phosphate metabolism in relation to type 2 diabetes and its vascular complications. Phosphates 90-99 insulin Homo sapiens 22-29 22339262-5 2012 We demonstrated the possibility of using these polymersomes as sugar-responsive delivery vehicles for insulin in neutral phosphate buffer (pH 7.4). Phosphates 121-130 insulin Homo sapiens 102-109 22066699-5 2012 RESULTS: Univariate analysis showed that serum phosphate levels correlated negatively with systolic and diastolic blood pressure, glucose values, waist circumference, insulin, and triglycerides. Phosphates 47-56 insulin Homo sapiens 167-174 22066699-7 2012 Multivariate analysis showed that insulin levels were correlated with serum phosphate concentration (r = - 0.24, p = 0.01). Phosphates 76-85 insulin Homo sapiens 34-41 21803140-3 2011 SBE4-beta-CyD increased the solubility and suppressed aggregation of insulin glargine in phosphate buffer at pH 9.5, probably due to the interaction of SBE4-beta-CyD with aromatic amino acid residues such as tyrosine of insulin glargine. Phosphates 89-98 insulin Homo sapiens 69-76 22151908-2 2011 RESULTS: The impact of a step-wise restricted supply of phosphate on the physiological state of S. cerevisiae cells producing human Insulin was studied. Phosphates 56-65 insulin Homo sapiens 132-139 22151908-5 2011 The re-distribution of the carbon flux from biomass formation towards increased glycerol production under low phosphate led to increased transcript levels of the insulin gene, which was under the regulation of the TPI1 promoter. Phosphates 110-119 insulin Homo sapiens 162-169