PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 12388765-0 2002 ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes. Phosphatidylinositol 4,5-Diphosphate 38-75 ADP ribosylation factor 1 Homo sapiens 0-4 9819391-3 1998 We have purified and cloned two variants of a 130-kDa phosphatidylinositol 4, 5-biphosphate (PIP2)-dependent Arf1 GTPase-activating protein (GAP), which we call ASAP1a and ASAP1b. Phosphatidylinositol 4,5-Diphosphate 54-91 ADP ribosylation factor 1 Homo sapiens 109-113 12213489-4 2002 Spermine-induced increases in PtdIns(4,5)P(2) content in permeabilized cells also occurred during GTPgammaS stimulation in the absence of PITPalpha, were augmented in the presence of PITPalpha, occurred in unstimulated cells and were additive to PtdIns(4,5)P(2) formation evoked by ARF1, another activator of phosphoinositide kinases. Phosphatidylinositol 4,5-Diphosphate 30-45 ADP ribosylation factor 1 Homo sapiens 282-286 9819391-3 1998 We have purified and cloned two variants of a 130-kDa phosphatidylinositol 4, 5-biphosphate (PIP2)-dependent Arf1 GTPase-activating protein (GAP), which we call ASAP1a and ASAP1b. Phosphatidylinositol 4,5-Diphosphate 93-97 ADP ribosylation factor 1 Homo sapiens 109-113 8793299-15 1996 In comparison, ARF1 activates PLD, producing PA, which is a known activator of phosphatidylinositol-4-phosphate 5 kinase, the enzyme responsible for PIP2 synthesis. Phosphatidylinositol 4,5-Diphosphate 149-153 ADP ribosylation factor 1 Homo sapiens 15-19 9268368-2 1997 Binding of the PH domain to phosphatidylinositol 4,5-bisphosphate (PIP2) greatly enhances Arno-mediated activation of myristoylated ARF1. Phosphatidylinositol 4,5-Diphosphate 28-65 ADP ribosylation factor 1 Homo sapiens 132-136 9268368-2 1997 Binding of the PH domain to phosphatidylinositol 4,5-bisphosphate (PIP2) greatly enhances Arno-mediated activation of myristoylated ARF1. Phosphatidylinositol 4,5-Diphosphate 67-71 ADP ribosylation factor 1 Homo sapiens 132-136 9268368-9 1997 We conclude that efficient activation of full-length ARF1 by Arno requires a membrane surface and two distinct protein-phospholipid interactions: one between the PH domain of Arno and PIP2, and the other between amino-terminal cationic residues of ARF1 and anionic phospholipids. Phosphatidylinositol 4,5-Diphosphate 184-188 ADP ribosylation factor 1 Homo sapiens 53-57 9065426-0 1997 Functional interaction of ADP-ribosylation factor 1 with phosphatidylinositol 4,5-bisphosphate. Phosphatidylinositol 4,5-Diphosphate 57-94 ADP ribosylation factor 1 Homo sapiens 26-51 9065426-2 1997 Because both Arf-dependent phospholipase D and Arf1 GTPase-activating protein (GAP) require phosphatidylinositol 4,5-bisphosphate (PIP2), Arf1 complexed with PIP2 has been proposed to interact with target proteins. Phosphatidylinositol 4,5-Diphosphate 92-129 ADP ribosylation factor 1 Homo sapiens 47-51 9065426-2 1997 Because both Arf-dependent phospholipase D and Arf1 GTPase-activating protein (GAP) require phosphatidylinositol 4,5-bisphosphate (PIP2), Arf1 complexed with PIP2 has been proposed to interact with target proteins. Phosphatidylinositol 4,5-Diphosphate 92-129 ADP ribosylation factor 1 Homo sapiens 138-142 9065426-2 1997 Because both Arf-dependent phospholipase D and Arf1 GTPase-activating protein (GAP) require phosphatidylinositol 4,5-bisphosphate (PIP2), Arf1 complexed with PIP2 has been proposed to interact with target proteins. Phosphatidylinositol 4,5-Diphosphate 131-135 ADP ribosylation factor 1 Homo sapiens 47-51 9065426-2 1997 Because both Arf-dependent phospholipase D and Arf1 GTPase-activating protein (GAP) require phosphatidylinositol 4,5-bisphosphate (PIP2), Arf1 complexed with PIP2 has been proposed to interact with target proteins. Phosphatidylinositol 4,5-Diphosphate 158-162 ADP ribosylation factor 1 Homo sapiens 47-51 9065426-2 1997 Because both Arf-dependent phospholipase D and Arf1 GTPase-activating protein (GAP) require phosphatidylinositol 4,5-bisphosphate (PIP2), Arf1 complexed with PIP2 has been proposed to interact with target proteins. Phosphatidylinositol 4,5-Diphosphate 158-162 ADP ribosylation factor 1 Homo sapiens 138-142 9065426-4 1997 Arf1 was shown to bind to PIP2 in Triton X-100 micelles with a Kd of 45 +/- 13 microM. Phosphatidylinositol 4,5-Diphosphate 26-30 ADP ribosylation factor 1 Homo sapiens 0-4 9065426-9 1997 These data support the hypothesis that PIP2 binding to Arf1 promotes interaction with Arf GAP. Phosphatidylinositol 4,5-Diphosphate 39-43 ADP ribosylation factor 1 Homo sapiens 55-59 9003363-7 1996 Since ARF1-regulated PLD activity requires phosphatidylinositol 4,5-bisphosphate (PIP2), the distributions of inositol lipids and the kinases responsible for lipid phosphorylation were examined. Phosphatidylinositol 4,5-Diphosphate 43-80 ADP ribosylation factor 1 Homo sapiens 6-10 9003363-7 1996 Since ARF1-regulated PLD activity requires phosphatidylinositol 4,5-bisphosphate (PIP2), the distributions of inositol lipids and the kinases responsible for lipid phosphorylation were examined. Phosphatidylinositol 4,5-Diphosphate 82-86 ADP ribosylation factor 1 Homo sapiens 6-10 9003363-11 1996 It is concluded that:(1) ARF1-regulated PLD activity is localized at endomembranes and the plasma membrane, (2) PIP2 is available at both membrane compartments to function as a cofactor for ARF-regulated PLD, and (3) in intact cells, formyl-Met-Leu-Phe stimulates PLD activity at endomembranes as well as plasma membrane. Phosphatidylinositol 4,5-Diphosphate 112-116 ADP ribosylation factor 1 Homo sapiens 25-29 7961749-9 1994 However, at later times (> 5 min), PIP2 caused a paradoxical loss of nucleotide binding to ARF1. Phosphatidylinositol 4,5-Diphosphate 38-42 ADP ribosylation factor 1 Homo sapiens 94-98 7961749-10 1994 PIP2 was found to stabilize the nucleotide-free form of ARF1 as subsequent dilution of PIP2 allowed ARF1 to bind GTP gamma S to high stoichiometry. Phosphatidylinositol 4,5-Diphosphate 0-4 ADP ribosylation factor 1 Homo sapiens 56-60 7961749-10 1994 PIP2 was found to stabilize the nucleotide-free form of ARF1 as subsequent dilution of PIP2 allowed ARF1 to bind GTP gamma S to high stoichiometry. Phosphatidylinositol 4,5-Diphosphate 0-4 ADP ribosylation factor 1 Homo sapiens 100-104 7961749-10 1994 PIP2 was found to stabilize the nucleotide-free form of ARF1 as subsequent dilution of PIP2 allowed ARF1 to bind GTP gamma S to high stoichiometry. Phosphatidylinositol 4,5-Diphosphate 87-91 ADP ribosylation factor 1 Homo sapiens 56-60 7961749-10 1994 PIP2 was found to stabilize the nucleotide-free form of ARF1 as subsequent dilution of PIP2 allowed ARF1 to bind GTP gamma S to high stoichiometry. Phosphatidylinositol 4,5-Diphosphate 87-91 ADP ribosylation factor 1 Homo sapiens 100-104 28923919-5 2017 We used this structure to analyze the interaction of uncomplexed Brag2 and of the myristoylated Arf1/Brag2 complex with a phosphatidylinositol bisphosphate (PIP2) -containing lipid bilayer, using coarse-grained molecular dynamics. Phosphatidylinositol 4,5-Diphosphate 157-161 ADP ribosylation factor 1 Homo sapiens 96-100 22688902-0 2012 Incorporation of a fluorous diazirine group into phosphatidylinositol 4,5-bisphosphate to illustrate its interaction with ADP-ribosylation factor 1. Phosphatidylinositol 4,5-Diphosphate 49-86 ADP ribosylation factor 1 Homo sapiens 122-147