PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 34052862-1 2021 Phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) is generated through phosphorylation of phosphatidylinositol 4-phosphate (PtdIns(4)P) by Mss4p, the only PtdIns phosphate 5-kinase in yeast cells. Phosphatidylinositol 4,5-Diphosphate 0-37 1-phosphatidylinositol-4-phosphate 5-kinase Saccharomyces cerevisiae S288C 143-148 34052862-1 2021 Phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) is generated through phosphorylation of phosphatidylinositol 4-phosphate (PtdIns(4)P) by Mss4p, the only PtdIns phosphate 5-kinase in yeast cells. Phosphatidylinositol 4,5-Diphosphate 39-52 1-phosphatidylinositol-4-phosphate 5-kinase Saccharomyces cerevisiae S288C 143-148 34052862-11 2021 These results suggest that PtdIns(4)P produced by either Pik1p or Stt4p is immediately phosphorylated by Mss4p and converted to PtdIns(4,5)P2 at the plasma membrane. Phosphatidylinositol 4,5-Diphosphate 128-141 1-phosphatidylinositol-4-phosphate 5-kinase Saccharomyces cerevisiae S288C 105-110 17392273-7 2007 Under these conditions PtdIns(4,5)P2 was not detected on vacuole membranes, and vacuole morphology was normal, indicating vacuolar PtdIns(4,5)P2 derives from Mss4p-generated plasma membrane PtdIns(4,5)P2. Phosphatidylinositol 4,5-Diphosphate 131-144 1-phosphatidylinositol-4-phosphate 5-kinase Saccharomyces cerevisiae S288C 158-163 24465216-5 2014 Here we show that PC(O-16:0/2:0) regulates the distribution of the PtdIns(4)P 5-kinase Mss4 and its product PtdIns(4,5)P2 leading to the formation of invaginations at the plasma membrane (PM). Phosphatidylinositol 4,5-Diphosphate 108-121 1-phosphatidylinositol-4-phosphate 5-kinase Saccharomyces cerevisiae S288C 87-91 19477927-4 2009 Defects in Mss4 function led to actin depolarization, an inhibition of secretion, reduced levels of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P(2)] in membranes, mislocalization of a pleckstrin homology domain fused to green fluorescent protein, and the mislocalization of Cdc42. Phosphatidylinositol 4,5-Diphosphate 100-137 1-phosphatidylinositol-4-phosphate 5-kinase Saccharomyces cerevisiae S288C 11-15 18208507-2 2008 PtdIns(4,5)P2 generation at the plasma membrane in yeast is mediated by the kinase Mss4p, but the mechanism underlying the temporal and spatial activation of Mss4p to increase formation of PtdIns(4,5)P2 at appropriate sites is not known. Phosphatidylinositol 4,5-Diphosphate 0-13 1-phosphatidylinositol-4-phosphate 5-kinase Saccharomyces cerevisiae S288C 83-88 18208507-2 2008 PtdIns(4,5)P2 generation at the plasma membrane in yeast is mediated by the kinase Mss4p, but the mechanism underlying the temporal and spatial activation of Mss4p to increase formation of PtdIns(4,5)P2 at appropriate sites is not known. Phosphatidylinositol 4,5-Diphosphate 0-13 1-phosphatidylinositol-4-phosphate 5-kinase Saccharomyces cerevisiae S288C 158-163 18208507-2 2008 PtdIns(4,5)P2 generation at the plasma membrane in yeast is mediated by the kinase Mss4p, but the mechanism underlying the temporal and spatial activation of Mss4p to increase formation of PtdIns(4,5)P2 at appropriate sites is not known. Phosphatidylinositol 4,5-Diphosphate 189-202 1-phosphatidylinositol-4-phosphate 5-kinase Saccharomyces cerevisiae S288C 83-88 18208507-2 2008 PtdIns(4,5)P2 generation at the plasma membrane in yeast is mediated by the kinase Mss4p, but the mechanism underlying the temporal and spatial activation of Mss4p to increase formation of PtdIns(4,5)P2 at appropriate sites is not known. Phosphatidylinositol 4,5-Diphosphate 189-202 1-phosphatidylinositol-4-phosphate 5-kinase Saccharomyces cerevisiae S288C 158-163 23781030-3 2013 In the yeast Saccharomyces cerevisiae, the sole phosphatidylinositol-4-phosphate 5-kinase (PI4P5K) Mss4p is essential for generating plasma membrane PtdIns(4,5)P2. Phosphatidylinositol 4,5-Diphosphate 149-162 1-phosphatidylinositol-4-phosphate 5-kinase Saccharomyces cerevisiae S288C 99-104 23781030-6 2013 We show that mss4-f12 cells have reduced plasma membrane PtdIns(4,5)P2 levels as well as a defect in its polarized distribution, yet Mss4-f12p is catalytically active in vitro. Phosphatidylinositol 4,5-Diphosphate 57-70 1-phosphatidylinositol-4-phosphate 5-kinase Saccharomyces cerevisiae S288C 13-17 17392273-7 2007 Under these conditions PtdIns(4,5)P2 was not detected on vacuole membranes, and vacuole morphology was normal, indicating vacuolar PtdIns(4,5)P2 derives from Mss4p-generated plasma membrane PtdIns(4,5)P2. Phosphatidylinositol 4,5-Diphosphate 131-144 1-phosphatidylinositol-4-phosphate 5-kinase Saccharomyces cerevisiae S288C 158-163 16856876-7 2006 Yeast phosphatidylinositol 4-phosphate 5-kinases Mss4 and Its3 are involved in resistance against disturbance of sphingolipid biosynthesis and maintenance of cell integrity through the synthesis of PtdIns(4,5)P2 and downstream signalling through the Rom2/Rho2 and Rgf1/Rho pathways. Phosphatidylinositol 4,5-Diphosphate 198-211 1-phosphatidylinositol-4-phosphate 5-kinase Saccharomyces cerevisiae S288C 49-53 16316315-3 2006 We have investigated the effects of weak acids on two stress-responsive signalling molecules, PtdIns(3,5)P2 and PtdIns(4,5)P2, which in S. cerevisiae are synthesized by Fab1p and Mss4p respectively. Phosphatidylinositol 4,5-Diphosphate 112-125 1-phosphatidylinositol-4-phosphate 5-kinase Saccharomyces cerevisiae S288C 179-184 16738335-10 2006 These findings, in combination with previous work identifying Slm1 and Slm2 as targets of Mss4/phosphatidylinositol 4,5-bisphosphate and TORC2 signaling, suggest that Slm proteins integrate information from a variety of signaling pathways to coordinate the cellular response to heat stress. Phosphatidylinositol 4,5-Diphosphate 95-132 1-phosphatidylinositol-4-phosphate 5-kinase Saccharomyces cerevisiae S288C 90-94 15741172-4 2005 MSS4 encodes a phosphatidylinositol-4-phosphate 5-kinase that synthesizes phosphatidylinositol (4,5)-bisphosphate (PI4,5P(2)). Phosphatidylinositol 4,5-Diphosphate 74-113 1-phosphatidylinositol-4-phosphate 5-kinase Saccharomyces cerevisiae S288C 0-4 14528019-8 2004 sec14, pik1, and mss4 mutants displayed decreased Spo14p PLD activity, whereas absence of Spo14p did not affect phosphoinositide levels in vivo, suggesting that formation of PtdIns(4,5)P2 is important for Spo14p activity. Phosphatidylinositol 4,5-Diphosphate 174-187 1-phosphatidylinositol-4-phosphate 5-kinase Saccharomyces cerevisiae S288C 17-21